LOCUS       DAOSZX010000001        53720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_501_length_53720_cov_20.366552, whole genome shotgun sequence.
ACCESSION   DAOSZX010000001 DAOSZX010000000
VERSION     DAOSZX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 53720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53720
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_53720_cov_20.366552"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK196_00005"
     CDS             complement(<1..189)
                     /locus_tag="PK196_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HON39838.1"
                     /translation="MSKERTLVLCVDRDDDLGFKAGIKGPIMGREACLHAATSLALAD
                     PEDSDVNALFETIKIYDEL"
     gene            complement(244..1104)
                     /locus_tag="PK196_00010"
     CDS             complement(244..1104)
                     /locus_tag="PK196_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil protein"
                     /protein_id="HON39839.1"
                     /translation="MLNDLIEKRKKILAESEQHKNRRNELNALASKYARERNELNAQT
                     REYVEEAQRHKEERDRINKEVQALKEERNGYNERANALFEEIEAFKKEHGNLQNRSIK
                     ELQKQIEHLEFKQQTEVYSTDKERELIEKIKHLKALVKEQEAELEQNKEMRAKLAEAR
                     ELRRRASEIHKEVTEKAELAQQHHDLMVEAYRKADKSREEADRAHQKFVEAQEAADEE
                     HRLFIACQKELRDYDKVISGLRKKTRKTKVTREQKAVRKEAERVFQQFRDGEKITTDD
                     LLLLQRAKLI"
     gene            complement(1473..2600)
                     /gene="ftsZ"
                     /locus_tag="PK196_00015"
     CDS             complement(1473..2600)
                     /gene="ftsZ"
                     /locus_tag="PK196_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HON39840.1"
                     /translation="MQTIINEALKHAEREKYLKSETDEVEDDILGQPRIVIVGCGGAG
                     NNTVNRLYHMQVTGAETIAINTDKQHLDMIRADKRVLVGKSLTKGLGAGGFPDVGRRA
                     AEMARPTLETLLCDADLVFITAGMGGGTGTGTAPVIAQIAKDQGAIVVGMVSYPFQVE
                     KARLLRAEEGLEALSNTADSVIVLDNNRLIKFVPNLPLGQAFSVMDQLIAETVKGISE
                     TITEPSLINIDYADVRAIMSKGGVAVMLVGESKQQNKAESVVHECLNHPLLDIDYRGA
                     TGSLIHITGGNDLTLQDAEEIASSLTYELDPHADVIWGARVNSDFEGRVRVMAIMTGV
                     KSAQILGNRRAAYEQVSARPLTSSNRRAARDAASGHLIDFM"
     gene            complement(2612..2788)
                     /locus_tag="PK196_00020"
     CDS             complement(2612..2788)
                     /locus_tag="PK196_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HON39841.1"
                     /translation="MDRITIRLPRQQVEMLEKLVEAGEFPTVSEAVRYSVRALLERHG
                     DRVLREADQISFNG"
     gene            2990..3991
                     /locus_tag="PK196_00025"
     CDS             2990..3991
                     /locus_tag="PK196_00025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HON39842.1"
                     /translation="MREYLPERAEHGGRVRWHRSREMRDEVLDFSANMNPYPPDIHWT
                     PDLSALKDYPDDRYEVLKEVIAGTFGRDAAEVTVGNGSVELIRAFCCAMLGPGDAACI
                     EMPTFAEYGVAVRLAGGRCVTGDDGATVRFLCNPNNPTGTLFSRAEVFRVLDAVAERG
                     GCLFLDEAFIELADPRQSLVDLPHENLFVLRSLTKSFAVPGIRFGYGFGSPELIDRIE
                     RARLPWTVNTFAESFAIEAFRHYDRLEESRKRIAEERAWLSGRLEGLGLVCVPPSANY
                     ILVGIPLESGVLSERLLSRGILVRDCRSFGLPRHIRVAVRTREENRQLIEALEACLH"
     gene            3979..4587
                     /locus_tag="PK196_00030"
     CDS             3979..4587
                     /locus_tag="PK196_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HON39843.1"
                     /translation="MLALIMAGGEGSRLGMGEKPLVNICGRPMVSYVIDAFLAADHEV
                     VVVASHRTPFTKNWCRAQGLTIYDAKGLGYVEDINAAAADLEEEGKPLFTCVSDLPCL
                     AVDIIADIEAAWRRSGLPACSTWVPRDLCLEHGCRTHYTEIIDGVPACPAGVNILTAS
                     REEGIPEEEVQVLLHDRRLAFNINTREELALVQRYLCGKHEG"
     gene            4745..5995
                     /locus_tag="PK196_00035"
     CDS             4745..5995
                     /locus_tag="PK196_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HON39844.1"
                     /translation="MRPCREIELEGHIIDSGIMTMVFDRIMDMGGEFEILTFNVGKLK
                     TDTSYARLRVTAPTEQQLDAILSELHRLGARTPEVSDVILVPAEGDRILPKGFYSTTN
                     HPTYVKYKGEWLPVENIEMDCHIVIDEKENRAICTPISKIRAGDLVVVSETGVRVIYP
                     ERPRKSSTFEFMHGTVSSERPSKAIITKIAREILKLKKEGGKIALVGGPAIVHTGAAD
                     ALATMIRDGYIDVLFAGNALATHDIESNLYGTSLGMDIRTGTLVTGGHKHHIRAISEI
                     MRAGSIKNAVDRGIITGGIMYECVKNEVPFVLAGSIRDDGPLPDVITDVVEAQDAMRR
                     HIHGLSMVVMVGTLLHSIAVGNCLPSYVKTVCVDINPASVTKLMDRGTTQAIGVVSDA
                     GTFLPLLCEQLEELERSQRVSGMQ"
     gene            complement(5971..6957)
                     /locus_tag="PK196_00040"
     CDS             complement(5971..6957)
                     /locus_tag="PK196_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HON39845.1"
                     /translation="MLSHLTEKPLASWRGKDRHEGRILDTLTVIFRSGGCSWNRCRMC
                     GYRHERYQDLAGAELAERIIRQVRWVKENFSDDDYQLVKVFTSGSFFDAREVPPAARH
                     AVAEAFRGKALIVETRPEYVEADGVREFREEIDTGTRAMPLTIAMGLETTDDLIREKC
                     IDKGFTYADFLRAAETAHAAGAGVKAYLLMKPPFLTEREARDDMIRSIRDLAPLVESI
                     SMNLCTVQSRTEVERLWREHAYRPPYLWSVLDVLIQSPVHVFCDPVGGGQMRGPHNCG
                     ACDSPIVKGIADYSLNNDAGLLRALAEMECGCKEEWEFVLEQEEPYCMPLTR"
     gene            6986..8071
                     /locus_tag="PK196_00045"
     CDS             6986..8071
                     /locus_tag="PK196_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39846.1"
                     /translation="MKRIALLALIAAVVLIAPSSAAYVSISAPQEIYVGDPLVVVGTS
                     TIEGQPKPSLSPGFSTEIVLYYAQYAKREIERKTIVVQEDGTFTATFETEGLEPGRYS
                     IEIIDPTKTTFGGSSKTQQLVTLIDRSGMITIDSPLTQEFDGTLDIRGSIADLGDAGV
                     QVRVEHDGFIVYGPTYLATDKNGAFSAEVPISAGGTYEVTFYDARGYIGSREFVVVGA
                     PEPTATGVIISATAPASRSAPAYFEVETRSGSVSITTSSGIDWVIEYIDEKNVRHKVN
                     EKGMLEPEKVEIAAGGGTIYLKVYPLSYSDNTTVQIFAANAEAVRVSRAAPGLFGDAT
                     PTPTQSTPLPPILALLAIFVVVLARRG"
     gene            8111..8195
                     /locus_tag="PK196_00050"
     tRNA            8111..8195
                     /locus_tag="PK196_00050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8145..8147,aa:Ser,seq:tga)
     gene            complement(8230..8634)
                     /locus_tag="PK196_00055"
     CDS             complement(8230..8634)
                     /locus_tag="PK196_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HON39847.1"
                     /translation="MVALTEEMKAAFRTMKAYPVATASKEGWPNVVPIAFVEIVDDET
                     IWIADNYMKKTLANVQENPRVAIYIWGPETKGCFQIKGDVEIRTSGPEFEKMQFTVRS
                     KMAKAPAKTLLIMKIREVYECTPGSRAGEKIL"
     gene            complement(9079..9780)
                     /locus_tag="PK196_00060"
     CDS             complement(9079..9780)
                     /locus_tag="PK196_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HON39848.1"
                     /translation="MMRINLTDDLLVIYNALRSAFGHQHWWPAETPFETMIGAILTQN
                     VSWTNAAQAVRNLDEAGLLDPHALAAAETSEIARLIVPSRYYNQKAERIREFSRVYVA
                     EFAADPGAMAAVDTDLLRRRLLAIRGLGKETVDTILLYACEKPVFVVDAYTRRVFSRY
                     GLLPEDASYDRTQRLFAENLEPDVELFNDYHAQIVHLGKTICRKSPLCDRCPIREVRE
                     TLCCEKTVSLAAGDP"
     gene            complement(9777..11369)
                     /locus_tag="PK196_00065"
     CDS             complement(9777..11369)
                     /locus_tag="PK196_00065"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HON39849.1"
                     /translation="MKVSLLQVNTVVGDLAGNAGRIAAGAREAARDRPDVIVAPELSL
                     TGCPLRDLLLRPGFAARALSALDELAASLAGAPPVIIGLPIANPAGTGRPAFNAAALI
                     REGEVAEIFRKPSISPGVLDEDRYFEPAGGEIPFIRFGGKTLGVVIGESICYEGPMPD
                     VIVNPVASPFAAGRGRLREEALSRFAREHGVAIASVNSVGGNDDLVFDGRSVVFNARG
                     TLVARGAPFDADIVTADLDNPAPQAVAADEYLPESEIWRALVLGTRDYVRKCGFRSVI
                     IGISGGIDSSLVAAVAASALGPENVLGVLLPSPYTSAANIEDAEQLADNLGIRRCCIP
                     ITPMMETFSSRLAGVFSGMPPDTTEENLQARIRATVLMALANKFGSMLLATGNKSEIA
                     VGYCTLYGDMAGGLAVIADLPKGMVYRVARWLNARRPVIPQRVLEKPPSAELRPGQVD
                     QETLPPYDLLDAILERWIDRLESPDDIIAAGYPPETVRKVVRMVELAEFKRRQAPPGI
                     RVTGRAFGTDWQMPIAAGRWWL"
     gene            11568..12920
                     /locus_tag="PK196_00070"
     CDS             11568..12920
                     /locus_tag="PK196_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON39850.1"
                     /translation="MGGLNSRRHWRTGGETHRLLLLAVSVIVVFAVNVAGLLSGIFTV
                     LPHLFYLPIVLGAYWYPRRGVPLSFGLAAGYLATTIFFAGLDMGLLIQTLARAVVFVA
                     IGAVISLLSLRLREQEERYRGVFDNSEAGTFIVASGVDGPRIEEVNYVGASLLGSTID
                     DLTGKPITLFFGDPALWERLEADSDRRGAVYGFETTLRRTDGETIRALVSAGRLQGGR
                     IVLTLIDITALKTAEETLRRTNAKLNMLGRLTHRDLAAAVSQLLAQITQGMRHFDDPA
                     FRRYLEELEESARVVQRRVEITRDYQDLGLQPPDWQPVQEVIRRAMSCLLIPPEVSVR
                     CWVERLEVFADPMLDRVFSNLIENAIRHGKTVSSVVITYQILDDGLAIYVEDDGVGVP
                     PEKKERIFDYGVGIDGGLGLFLVREILAITGITIRETGTPGEGARFVIHVPPGGYRIT
                     "
     gene            12933..14162
                     /locus_tag="PK196_00075"
     CDS             12933..14162
                     /locus_tag="PK196_00075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON39851.1"
                     /translation="MFPKVLIPTDLSAASATVVERIAEVPGVREVILFHALRSSTGLS
                     PADEETLQRMQETVQRQGYPAVVVVEDGMAGVPARILQTATERDVDLIAMGVRDPGLL
                     RNLFSGNVAATVLRDAHVHVLILPQSARRGPPLGARLLVPTDLSDPAPEIRSLLRESD
                     GEGMAVLLHVIEPGRTEQEARDQLEALRAELAPLRRDVEVMARTGDPARTICAVAEEI
                     EASMVVIPRIGRRDAAGAATLGSVTGAVIGCVKRPLLVIAISIHVQIETRELRSEEFG
                     LAEEIWEDYHGLKADPKTDRIFGVFADGRLVSVARCRRHPDGYEVDGVFTPVRFRGRG
                     YARRAMDALVEACQHDTLYMHAVRNLVDFYGKYGFVSIPESELPPTIRGRYAFALGEM
                     EGANVQPMRRPPVRFMR"
     gene            14288..14800
                     /locus_tag="PK196_00080"
     CDS             14288..14800
                     /locus_tag="PK196_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON39852.1"
                     /translation="MLIGILADTHDNLEKVDAAVRSLNAKGVEMVLHAGDYVSPFVIP
                     RFASLQSPMIGVLGNNDGDHRLLQRRFEEHDRFSLRGRFACISADGLAIGLLHGDDHE
                     LLQALITRKVFDVVVYGHTHQAEVRSLPGTLVINPGEACGYLTGRSTVAVLDTVTRSV
                     ELLTPGHEEL"
     gene            14904..15380
                     /locus_tag="PK196_00085"
     CDS             14904..15380
                     /locus_tag="PK196_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HON39853.1"
                     /translation="MEIVKIPNMDRAEYDKLIEEGFLSRIAFQGAKYPYIAPFLYVFD
                     GKFLYFLATKYGRKNNLFRQHPYVSVEIEKYSSDLSCYTFVTMQGYLVQVEDAIEKKI
                     IREKFVEMIGKHNLSRNILAALGHMPEEPIEAIASEERSNIWKLTGVKDIVALKNL"
     gene            15505..16443
                     /locus_tag="PK196_00090"
     CDS             15505..16443
                     /locus_tag="PK196_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HON39854.1"
                     /translation="MIHISEGWDDLAAALIESGLRESVYIMGATDTGKTTLCRYLLSR
                     TSAHVRTGCVDCDTGQSWIGPPTTEGMTIHPRPSKPVLRFVGSTSPRGHLVQTLTAAK
                     RLAEWALKDGARITLIDSPGLVHGGVGIEFQIQMIDLLRPDHIVALQRGRELEGVLAN
                     FARHPTIQTHRFPVSTAVLPRTAAERRRYREERFTAYFAGAQVQEIALREPGLQGRVP
                     DPENRSGVAGRLVSLNDPENFVIVLGIVSEFDPKGRTLTVFAPPFDTTSVASVRFGSV
                     SLDLEAPPGTMECHRFEASTQGQTRARPHTPPPGLL"
     gene            complement(16388..17641)
                     /locus_tag="PK196_00095"
     CDS             complement(16388..17641)
                     /locus_tag="PK196_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HON39855.1"
                     /translation="MGTFIPLIVLIIVFILIAVRRIGSVDLRIWQVMLIGAVVVVLTG
                     SITPGDALASINTDVMLFLFFMFILGEALATSGYLYHLSVRLFCRAESVGSLVFLILI
                     GAGGLSALLMNDTLAVVGTPLMLYFARRHGISAELLLLTLAFAITTGSVASPIGNPQN
                     LLIALSGGVTNPFITFPLYLGIPTVISLLLTYWFLRQAFPDEFKSAGQLAHCEEEIHD
                     PVLAALTRLSLVLLVIMIVVRVAVVMLVPDVEIPLTWIAAVAAIPILIGSRRRFEILR
                     RIDWPTLVFFAALFVLTASVWESGVFQPLVEGRSLDPGAVSVIIALGVIVSQFISNVP
                     FVALFLPVLSHAGASTVGMMALAAGSTIAGNMLILGAASNVIIIQGAEREGETLTFAR
                     FARVGVPLTIAQAVVYAVWLSFVPA"
     gene            complement(17716..18690)
                     /locus_tag="PK196_00100"
     CDS             complement(17716..18690)
                     /locus_tag="PK196_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HON39856.1"
                     /translation="MKKIVVHVRKDGYEKVKEALEGVHYTEILLQDVYVFTIYAPDEH
                     LDDLIEKIQNVLDLRYNENLIEVSTPEFVISSRLKRAVKNIEKKEEKTPVEKLLDSIR
                     PYGELDLEKLALTTIAGLIALSGLLLNNPVIIIGAMLLSPIIGPIYGFSIYAALGMVR
                     ESLRCLGVLAALLLSVFALSAVAAFLINLVIPLTLTAEIASRVVVNPIYTLMAVLLGF
                     AAVLAFNRGTSEVIAGVAIAAAIIPPMAVMGIVLVIQPPSLIASALLVGGQVVGLIVG
                     ALLAVAMLKVRPRSSRDRAIAQRYMKRSAALIVLLFALLLWITLIMVE"
     gene            complement(19193..19855)
                     /locus_tag="PK196_00105"
     CDS             complement(19193..19855)
                     /locus_tag="PK196_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HON39857.1"
                     /translation="MRLNWVGHACFLLEGSKRVLIDPFMPEGALRTEPDMVAVTHAHA
                     DHLGIAVRLKKKTVAIAELAHYLTTKGVPAEPMNIGGSITVDGVRFTMTPALHSSGLD
                     GDAPGFYGGAPAGFVITMDGISVYHAGDTALFSDMQLIRDLYRPDVALLPVGGCYTMG
                     PEEAMIAARYIGARLVIPMHYNTFPAIQQNLDEFKKTIERTTPIRVALLSPGESIEVN
                     PE"
     gene            complement(19909..20517)
                     /locus_tag="PK196_00110"
     CDS             complement(19909..20517)
                     /locus_tag="PK196_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HON39858.1"
                     /translation="MAEDTRKRQLLELFTTITNKKAIVEPMRKVHGTLRDRDAVEREI
                     ALIMREILDRGYFKTKLTPRQLAKLVVAYYDGMNDTEIARALGDEKLSKTVARARVRL
                     KLFRDLDFKMPFDQEELLKLLESGKTLKEISEELNVSPSTLREYRHVIEQRSDTTLDP
                     YLERIRDVMEDRDLSEAMTRGVANDGLSEAIDITEAIDMGEF"
     gene            20833..22563
                     /gene="glyS"
                     /locus_tag="PK196_00115"
     CDS             20833..22563
                     /gene="glyS"
                     /locus_tag="PK196_00115"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HON39859.1"
                     /translation="MAETSDIYEKVMDVARRRGFVWPSSEIYGSVAGFIDYGPLGAML
                     KRNIENLWRSFYVFQEGYYEIECPTIGTEAIFVASGHVKEFADKMVQCPHCGEYLRAD
                     HIAQEHGIENAGTLSAEALQAALRGLPCPSCNEVLGEAGVFAFNLMFQTTIGPGSQRK
                     GYLRPETAQGIFTDFPRLLRFYRDKLPFGAVQIGKSYRNEISPRQGMIRLREFSQAEA
                     EIFVHPDEKKHPSFSRYADYAVPLLTIAGQVENREPVTMTMHAAVDDGLIANEYVAYY
                     IALTHQILTSIGIDPAKVRFRQHLTEERAHYAADCWDAEVYSERFGWVEVVGIADRTD
                     YDLRSHAEHSGTSMTIFVPYDEPRLVKKRRIVPDMGVIGPRFREKAKAIVEALAVSEP
                     GEDGARITLDGEEIFIPADLYQVREEMVEVRGEDVMPHVIEPSYGIDRMIYAALEHSY
                     AEDEVDGEIRRVFRFPPAVAPIQVAVFPLMNRDGLDTIARRIAEALLQHRILAQYDDS
                     GAIGRRYRRQDEVGTPFAVTVDHDTLEDNTVTIRDRDSMEQVRVTIERLPGTLSGLIA
                     GKIKFHELKA"
     gene            22560..24791
                     /locus_tag="PK196_00120"
     CDS             22560..24791
                     /locus_tag="PK196_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON39860.1"
                     /translation="MKYVSHPLIRPQSIEERRYQISIALRALDMNTMVVLPTGLGKTA
                     VALIVAASRLYSHQGKVLVLAPTKPLVEQHLRFFRQHLLLSEGTEPEETDFAMFTGAT
                     PPDERAQAWEKCRICFATPQVIKNDCLAGRYSLTDVVLLVVDECHRAVGNYAYVFLAD
                     HYCNTAKNPLVLAMTASPGGDPLKVQEVCNNLHIEAVETRVETDEDVLPYIHEREIHY
                     IDVYLPPELQEALVTLKGLVESRLERLAKLNYQVPKADKLSIKALNALNAQIQQRIQA
                     RDPSGFMAASLHAECMKLRHAISLAETQGSEALKLYLARLGAEGASSAGSKASKRLVS
                     DPAYRRLVETAAGWREELHPKVSIVRELVEAQLAAHPESRIIVFATYRDTVQTLVDTL
                     RAAGIPCERFVGQASRDAERGLSQKEQIATLARFRAGELKCLIATSVGEEGLDVPSTD
                     MVIFYEAVPSEIRSIQRKGRTGRSGSGTIIVLVTKGTSDETFRYVSQNRERAMMRGIR
                     KMSAAPPHNPATRQTEILAFASPGPAITVDDRETSSRVVERLHQLGASIALERLDIGD
                     YAIGDRILVERKTVQDFVTTLVERDLFGQIQAIADAVPRPVLIIEGSDDLYAVRNIHP
                     NAIRGTLAAIMVDMGVALIRTRDPDDTAETLYVLAQREGGERRERKAHPKKSYRSSRE
                     EQEYVLAAFPNVGLKSARLLLEHFGSLKAVIEAEPGELMAVDGIGEKTASAIWDLAHR
                     PYL"
     gene            complement(24782..25162)
                     /locus_tag="PK196_00125"
     CDS             complement(24782..25162)
                     /locus_tag="PK196_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HON39861.1"
                     /translation="MTVERADEVMAEYLLKGGKMLAKSCKVCGYPLFEYKGETQCVIC
                     PLLESEEPHPGSEPSAPAAPAVQIPQTAAAAMEPQRPVAAEIERTIVHLCERVRSERR
                     AEDCLTLMKAVERGARALARLGQR"
     gene            25225..25488
                     /locus_tag="PK196_00130"
     CDS             25225..25488
                     /locus_tag="PK196_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HON39862.1"
                     /translation="MASPDETIQICIQMLQNISEDSTIPRNIRRAADSTKSVLLDESQ
                     SIGLRAATAISMIDEVSNDPNMPVHARTRIWELVSQLETVPLD"
     gene            complement(25577..25999)
                     /locus_tag="PK196_00135"
     CDS             complement(25577..25999)
                     /locus_tag="PK196_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HON39863.1"
                     /translation="MHRRTIQVNTHGEGEIVNLTPPVAETVAESGVREGLANIFVSGS
                     TAAVTTIEYEPGVLSDLCRALSVIAPEGIHYAHDAAWGDGNGRSHVRAAIVGPSLTVP
                     IIGGKLACGTWQQIVLLELDVRPQRQRTVHITIMGEDR"
     gene            26393..26617
                     /locus_tag="PK196_00140"
     CDS             26393..26617
                     /locus_tag="PK196_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39864.1"
                     /translation="MVDVPYRWNSRRDVDEAVVVVMNTMDSEHEIGGWLMETLQGAIN
                     DSDPELVKHFFSELEQHRPEALRYFAKPEF"
     gene            26763..27077
                     /locus_tag="PK196_00145"
     CDS             26763..27077
                     /locus_tag="PK196_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39865.1"
                     /translation="MPAKSEFGRGFIVNLMLLSRHFGLPPERAFYGAADHLNDFILPE
                     RFRGTEIEELVERLRKAVIWHQPGIMDKEDAADIKHLLNRLAIAIDRELGIAEPDVGK
                     YD"
     assembly_gap    27491..27590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27690..29660
                     /gene="gyrB"
                     /locus_tag="PK196_00150"
     CDS             27690..29660
                     /gene="gyrB"
                     /locus_tag="PK196_00150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HON39866.1"
                     /translation="MTETYDASHITVLEGLRPVRERPAMYIGSTDTRGLHHLVYEVVD
                     NAIDEALAGFCDLVLVTINRDGSVTVDDNGRGIPVDLMPRYGRSALEIVLTVLHAGGK
                     FDKNTYQVSGGLHGVGVSVVNALASWLDATVYRDGNVYTMQFRRGEVSRPLESRPETE
                     DEMRARYEARYGTPAPDEIDYGRLRGTRITFQPDRSIFETVEFDYDVLDHRLRELAYL
                     NSGLKIVLRDERTGDSATYCFEDGIRQFVAHLGEGKEKLHDEIIYFQKSDPESLVEVE
                     VALQYNNSYTETVFTYVNSVNTREGGTHLEGFRSAITRAINNAARRNNLIKNSDTLFR
                     GEDVREGLVAVISTRVANPQFEGQTKMRLGNSNVRGIVDSLVYSSLTDYFEEHPKTLQ
                     AIVEKAMAAARAREAARSARELARRKSSLESTGLPGKLADCQERDPAKSELYIVEGDS
                     AGGSAKQGRDRKFQAILPLRGKILNVEKSSEHRILKNAEIQALVSAIGTGIGDTFDAE
                     RARYNRIILMTDADVDGAHIRTLLLTFFYRYMTGLIEKGFIYIAQPPLYRVARGKQER
                     YAYGDEELRAILADWGEKGISIQRYKGLGEMNAEQLWSTTMDPANRILKQVRIEDAAY
                     ANTIFEKLMGEDVDARRDFIRRHAKEVSNLDI"
     gene            29650..32079
                     /gene="gyrA"
                     /locus_tag="PK196_00155"
     CDS             29650..32079
                     /gene="gyrA"
                     /locus_tag="PK196_00155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HON39867.1"
                     /translation="MTSEQQVVPVNIEEEMKSCYIDYAMSVIIGRAIPDVRDGLKPVH
                     RRTLYAMWEMGNTSDKPYRKSARVVGDVMGKYHPHGDAAIYDTLVKMAQPFTYRYPLV
                     DGQGNFGSIDGDAAAAMRYTEARLTKLSEELLADIDKKTVDFTPNFDGSLEEPEVLPA
                     RIPNLLINGSSGIAVGMATNMPPHNLREVCEAICRVIDNPGLSTDELMRIMPGPDFPT
                     GGIIMGTEGIRDAYLTGRGKCVIRGVAEIEEEGRTPRIVITEIPYQVNKARLIEHIAS
                     LVRDRRIEGISDIRDESDREGMRIVIDLRRGVAPQVILNYLYKHTALESSFGIINLAI
                     VDRQPRTLNLKEIIGEFIQHRVEVVRRRTEFDLAKAQERMHILRGLLVALDNIDAVVA
                     TIRASRTTEEAQKALVAQFALDEVQADAILKMQLRRLAALEQQKILDERDVLAAEIER
                     LTTILSTEANILAEIRKELRDIAARYGDDRRTRIVEAAETLEKEDLIEDKPMLVSLTA
                     SNYIKRIDLDAYRNQRRGGRGVIGMATKEDDGVERVFAASMHDHLLCFTDRGRVYWLK
                     VYDLPEGQRTGRGKALVNLLPLTGDERVTAVIPVREFQPDRYLFFATRGGTVAKVALD
                     EFSRPRQTGINAIKLRDGDALVDVNMTDGNQELILTTRLGQSLRFHEDSVRPVHRNAM
                     GVRGIRLRNGDALQSVTVVEGDHLLTITEQGFGKRTEFDEFRGHGRGTLGVRNIVVDA
                     RGGGVVGSMAVYENDEIIVMSASGIVIRTKVSEISIQKRGTRGVRIMRLDDGDRVVGF
                     TILNSEDRDEE"
     gene            complement(32316..33131)
                     /gene="cbiQ"
                     /locus_tag="PK196_00160"
     CDS             complement(32316..33131)
                     /gene="cbiQ"
                     /locus_tag="PK196_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HON39868.1"
                     /translation="MIEDLYSIERSAYGKSVIHRLDARIKLLLALAAIVAIVALPYST
                     KVYTLGAAFLALFLLLWAASRLSPLVYLRRLALLMPFGIFLVGFQAFVKNPYYDTFHT
                     VATLPFGLEIYAESLEFASILFVKFIVSISFIILLSSTTRMQDMIEAAGRLGLPREFT
                     LTLGMMVRYIFVFAEMFGRIRTAMSTRCFDPLDRSLPYRYRLHQVGYTVGTMFIRSYE
                     QGERTYTSMLCRGYGGKAGLYIRKKPLNTRERAFAAATLGFIVLSTVLIYLHP"
     gene            complement(33128..33961)
                     /locus_tag="PK196_00165"
     CDS             complement(33128..33961)
                     /locus_tag="PK196_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON39869.1"
                     /translation="MHLIETRELTHVYRGSVRALEGVNFIAERKARIAVIGPNGAGKS
                     TLFKHFNGILKPTSGGVLVRGEPITKDNLREVRKFVGIVFQNPDDQIISPTVEQDIAF
                     GPINLGLDEETVAHRVEEALRLLGIEDLRERAPHHLSGGEKKRVAIAGILAMEPQVLV
                     LDEPTAGLDPQGVDDLVAFINRLPEDYGMTVIFSTHHLDLVAEMADFIYVMDRGKIMG
                     SGTVEEVFARPELLTRAKLDVPPIPRLIRSLQENGVEIEMAYTYKDAEKAFLDAYMRE
                     R"
     gene            complement(33993..34343)
                     /locus_tag="PK196_00170"
     CDS             complement(33993..34343)
                     /locus_tag="PK196_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HON39870.1"
                     /translation="MEAKRFVIIGIAVALVIAIAAPFLASSNPDGLESAFFSMYGAKP
                     FMGSDLDEEAAAAAEEEVVAVTGNDFSHEPLMPDYSIPGMDKAGEVLAIVIGTLLMLG
                     LVFAVAKVSARPDN"
     gene            complement(34343..34981)
                     /gene="cbiM"
                     /locus_tag="PK196_00175"
     CDS             complement(34343..34981)
                     /gene="cbiM"
                     /locus_tag="PK196_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HON39871.1"
                     /translation="MHIPDAFIPIGQALVYWVIALIFVALALRWARRSMGEEKVPLVA
                     VLAAGIFAIQALNVPIPGGTSGHMVGAALAAIVLGSPYAGVFILTLVLLVQGVIFGDG
                     GITVMGANIINMGVIGGFIGYYGYTAVRRLISNAYAAAFIAGWASLFISAILCAVELA
                     IAGTFPLTVGLTMMGAYHAIIGLIEGGITAVALYLIASARPDILEHPLGVGA"
     gene            complement(35045..36211)
                     /locus_tag="PK196_00180"
     CDS             complement(35045..36211)
                     /locus_tag="PK196_00180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON39872.1"
                     /translation="MRRDKLTWDVIVVGAGPAGSAAARACAEEGLSTLCIEEHGTIGH
                     PVQCAGLLSTSAFAECGVSRRSILNEVSGARMFSGLGGEISFDAGVTKAFVVDRGLLD
                     REMAEKAADAGAEFRLKTSAAGIRGTALLTRGVRGREEFPFRLLIAADGPRSSISRML
                     GFRRPQVYLAGIQAEVPCSIDPRCVELHPNASPDFFGWAIPVSPTRARVGLCAREHAK
                     EYFDRFIVRYGGSMLHLVTGVIPLGVMPQTCGHRTFIVGDAAGFAKPTSGGGVYTGVR
                     SAKHAAAVAAACCSAGTFDGEHLREYERRWREDFGRELDIGMKALRLRQRMTPEEIDR
                     LCRALDSPDVIRTIVEHGDMDRPVSLLRKLALKPAVVRAMGILFASEVRRIITG"
     gene            complement(36477..36701)
                     /locus_tag="PK196_00185"
     CDS             complement(36477..36701)
                     /locus_tag="PK196_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39873.1"
                     /translation="MKRISIYLIFVAIWVAASAAVAAFPTILTPVAGVLNAPLNETIA
                     VFLSLMLVLTIIFLALIGLEAGRSVAEHLQ"
     gene            complement(36706..37296)
                     /locus_tag="PK196_00190"
     CDS             complement(36706..37296)
                     /locus_tag="PK196_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39874.1"
                     /translation="MKFVVDREELLAASRREIYDSVPKIVVGIAVAFLIWLFGVLIFQ
                     PLARMLGDPLIFSLIGLGSLISAIILIALLLIFIGIFKEVVDITNALAGYAAAAYSRK
                     ETPDEKLERYQRAFRGIAYVLLAVILFLFLLPFIAGIIPVLAGIVLVVLVIWAILVLF
                     RTGRLFSVEIERWAADVTRKIESHREEMEWNEGREE"
     gene            37532..37894
                     /gene="pth2"
                     /locus_tag="PK196_00195"
     CDS             37532..37894
                     /gene="pth2"
                     /locus_tag="PK196_00195"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HON39875.1"
                     /translation="MPEEREFKWKQCLIVRTDIKMTCGKKCAQIAHAAVSAFEKADKI
                     TKKAWLDEGQKKVVLKVPSERDLFELKTIAERAGIPASIIQDAGMTEIPPGTVTALGL
                     GPARSEDLDRITGDLPLL"
     gene            37891..39162
                     /gene="truD"
                     /locus_tag="PK196_00200"
     CDS             37891..39162
                     /gene="truD"
                     /locus_tag="PK196_00200"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HON39876.1"
                     /translation="MMPSTHPLERDLGMRYYASDTPGIGGRLRSTPADFIVEELPLPI
                     PDQEGPYLICRLTKINWELQRAVKEIAKRLGISHRRISWAGTKDKNAVTTQFISIYDV
                     SLDAIERVNIPDISLEVVGRSQHPLRLGGLAGNRFEVMIRECLKEDLPARVQAVTEAV
                     SAGIPNYYGLQRFGVIRPVTHVVGERILKGDYEGAVAAYIGRAYPMEPEEVQQARTRF
                     LETGDARAALADLPVRMTYERAMANHLAANPGDYAGALRVLPPKLLSLFVSAFQSCLF
                     NHALSARIDAGLSLTEPEVGDRLLFENGREDVVTARNQQAARIQIRRGRCRIALFIPG
                     AEPVRVYGPMDDILHDLMQGRGIDAADFERASRFVETAFSGVLRAISLSTDVEAEVAG
                     TDLRLRFSLPPGHYATTVCREYMKAEPYLMI"
     gene            complement(39222..39914)
                     /locus_tag="PK196_00205"
     CDS             complement(39222..39914)
                     /locus_tag="PK196_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HON39877.1"
                     /translation="MPQDDRPHVLMMSEITVDGKLTLKRGASSKILMKYMAPETEVLL
                     HRTRAECDAIMVGANTIRIDNSFLTVRLVEGKNPLRVIPTSRGEIPPGANILGPDART
                     LIVVSEAAPPENIARLRDAGVDVVVAGTSQVDLPRLMQILKQDYGVDRMMIEGGPTLN
                     WSMLHHHLVDEIRLIHLPFIVGGADTPSLVGGMHIETEEEMFRLSLKRYYMCGSNLVT
                     EWEVLYRPDSGE"
     gene            40056..40688
                     /locus_tag="PK196_00210"
     CDS             40056..40688
                     /locus_tag="PK196_00210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HON39878.1"
                     /translation="MNITSLRPKFVKCTEPIALFCIRLGVTPNQISVLSLLFGFGCAI
                     AFAGRHFAAGSLFLLISAVLDLVDGSVARRNNTGSRFGAVFDWIADKYVDAAVILGVG
                     FSGIPIVSHIFDVPAVADFGVVGLALAGSLLNTFIKPVTYAEIGYSERIAGKIEDPLE
                     GVGFFGRPETILVLLIGGMTGFLWVAVILIAVCTNLSALQRIVYLYRRYS"
     gene            complement(40825..41649)
                     /gene="artA"
                     /locus_tag="PK196_00215"
     CDS             complement(40825..41649)
                     /gene="artA"
                     /locus_tag="PK196_00215"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HON39879.1"
                     /translation="MSDYLVLISCICFLAFLIPWRYRNYFAIGGWVSIVGYLFAEFPR
                     YISLNNFVYPTLAILSLPLLYITIKYLLRDDPRVIQVSMIAAVAFLVYAPFGYIPALG
                     NWLISAVAGQTSAVLSAIGYPVNFQAWNMLERNGFQTEIVLACTGIQSIAIMLGVAWG
                     VKSTLKQKIAGFFIVFPTIYILNILRNVFVITAYTEQWFPYLPEIASNGEFGYESFFW
                     AHNVMAELGALVVLVALAYALFTAIPQLATLANDLYLLYRGELERLLRRGPEKTGT"
     gene            41781..42095
                     /locus_tag="PK196_00220"
     CDS             41781..42095
                     /locus_tag="PK196_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HON39880.1"
                     /translation="MMFCPQCKGLMISSGGQLKCKKCGYIRDINASDQLKKTDRRVEK
                     EITIVDAEKEIATLPTTTIKCPDCDCTTAYWWLRQLRAADESEVRFFRCTACGKTWRE
                     YD"
     gene            42263..42823
                     /locus_tag="PK196_00225"
     CDS             42263..42823
                     /locus_tag="PK196_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HON39881.1"
                     /translation="MLLFIDTETTGTFRFNHPPPRLVEVAWLVCEESGEVVERGDRIV
                     RPDDFTIPAGASRIHGITTGYARRAGVSLLEVIEALSIAATRSSTVVAHNLRFDRAII
                     AGECRRSGVSDPLSTLPGICTMESTPSVCRIRRGGGSGYKWPALSELYASLFGVPYEG
                     AVHRASGDVEVCAKCFFALKERGFWR"
     gene            43303..45201
                     /gene="acs"
                     /locus_tag="PK196_00230"
     CDS             43303..45201
                     /gene="acs"
                     /locus_tag="PK196_00230"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HON39882.1"
                     /translation="MAEKFDVKLEEARYYMPDASYKEQSWIGDYNRAYQEFLADPDGF
                     WDRIAHELEWFQPWDRVKEWDYPYAKWFTNAKLNITYNCLDRHVNNQRRNKVAIMWRG
                     ETEGEEEVYTYRQLLQAVSQFANGLKSLGVGKGDRVCIYMPVVPEQIIAMLACARIGA
                     IHTVVFGGFGVSALKQRIIGTGAKVVVTADFTYRRGKAIPLKNIVEEAVVNVPSVERI
                     VILRRDEKKPVELHPEIEIDFHELMEEADRECPPEVMDAEDPLFILYTSGTTGSPKGI
                     VHTCGGYMVGTYYTTKYVFDIKDNDVYWCTADPGWITGHSYGVYGPLLNGATFLIAEV
                     TPDYPTNGTYWDIIQDYGVTIFYTAPTAIRMFMRVGEEWPNRYNLSSLRVLGSVGEPL
                     NPEAFEWFYHVIGKTRCPVVDTWWQTETGMHMITTMIGEPMRPGFAGKPIPGVVADVV
                     DHNGNPVAPGTGGFLVIKEPWPSMMRTVWNDDERYRKYWYTIPGCYTAADLAVKNHDD
                     YIMILGRSDDLIVVAGHNIGTAEVESALVSHEAVAEAAVIGKPDQLKGNIIKAFVILK
                     NGRVPGEKLKKDLVYHVRMTLGPIAIPSEIEFVDSLPKTRSGKIMRRVLKAQELGMDP
                     GDISTLEE"
     gene            45244..>45503
                     /locus_tag="PK196_00235"
     CDS             45244..>45503
                     /locus_tag="PK196_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HON39883.1"
                     /translation="MSEHRPEESLKIENIVASAKVTDSLDLPSLASRLKDAEYNKKRF
                     PGVVLRMQDPKIAALVFGSGKVVLTGAKSIDSLSRGLQILTD"
     assembly_gap    45504..45603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <45604..45962
                     /locus_tag="PK196_00240"
     CDS             <45604..45962
                     /locus_tag="PK196_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HON39884.1"
                     /translation="GAKSIDSLSRGLQILTDQLRALGIEIPGNPEYKIQNIVTSADLG
                     TPINLNKIAVGFNLDRIEYEPEQFPGLVYRLDDPKVVALLFGSGKLIITGGKVPDDAR
                     RAVERIVSELSSLGLL"
     gene            46133..46513
                     /locus_tag="PK196_00245"
     CDS             46133..46513
                     /locus_tag="PK196_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON39885.1"
                     /translation="MGPKTTKSFTPRDEEVAELLTDIGIKRNVARVLVFLTNNGEATS
                     REIERGTDLRQPEVSIAMRYLKECNWIDVRESKSESKGRPVKIYTLARPISEIMDTIE
                     YEKKKEARHQLELIQRMRDCISTQ"
     gene            complement(46502..47200)
                     /gene="rpiA"
                     /locus_tag="PK196_00250"
     CDS             complement(46502..47200)
                     /gene="rpiA"
                     /locus_tag="PK196_00250"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HON39886.1"
                     /translation="MSALDPVAAKRIAGYRAAGEVRDGMVVGLGTGSTVFFAMERLGE
                     RITEEGLSIAGVPTSYQAAMRARRYGLPLTDLDEHPELDLAIDGADQVDPLFRLIKGR
                     GAALLREKCVCDAAREVLIVVDPSKMVETLDAGVPVEVLPFAAGTVFRRLEALGADAV
                     LREGVRKDGPVITDNGNFIIDCGFGAITDPGRLEVEIGSIPGVMGCGLFSIYGEKITV
                     IVGEENGCRILSLG"
     gene            complement(47197..48012)
                     /gene="surE"
                     /locus_tag="PK196_00255"
     CDS             complement(47197..48012)
                     /gene="surE"
                     /locus_tag="PK196_00255"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HON39887.1"
                     /translation="MKRRILLTNDDGITSTGLWAAYNALAPLADVTVVAPATQQSAVG
                     RSISIFEPIRANRVMINGVTAYSVGGKPTDAVIIGLFALRLNPDLVVSGINIGENLSF
                     ESVMTSGTIGAALEASNQGVPGIAFSLQVEDQGDKFDEPSGITRRFSGAKRVVREVCG
                     KVLETGFPAKAHVINVNIPAEVRGGYEITRLAAKLFHTGVEERFDPRGRPYYWIDGPL
                     YEDAEEGTDVHAVQQGNVSITPITLDCTAFDAAEDLQRIFGEMIAGEREVSLP"
     gene            48096..48986
                     /locus_tag="PK196_00260"
     CDS             48096..48986
                     /locus_tag="PK196_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HON39888.1"
                     /translation="MKVFLAEYTVCNDPDLAPEGAAMLDTLTGSFERCGHEVFTPEKP
                     DLEAEIRRLAPQSDAGLVIAPDHLIFRYTHLLEQFTHNIGCGSMNAAVCANKPRTAAI
                     LSRNGIPVPPPPAAGGRRVVKPIMGCGSRGVRLTEGEPGEGEFAEGYVDGEALSVSLV
                     GSRVTGNVCEFYSGAPPLLLAINRQEIAVSDDGLFHYMGGETPVHPEREDEIVEAAIR
                     TMNVLGCQGYAGIDLIAGDRIYVLDVNPRPTTSLVGIAAVMKEEIADILLRASYGDAP
                     GEVHLSGRVRFDRDGVIHRL"
     gene            48983..49894
                     /locus_tag="PK196_00265"
     CDS             48983..49894
                     /locus_tag="PK196_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HON39889.1"
                     /translation="MIGIDIGGANLKIVDEDGVHIHYCPLWQGAPLAELLRDCRRPAA
                     AVMSGELADCFPTKMEGIRWIVGTVLEAIPDALFYGIDAAFHTRPVPELAAANWLASA
                     DHLREDYPDAILLDVGSTTADIIPLNLFDDLKGLTDTRRLQKHYLVYTGLLRTNVATL
                     LRSVTLDGTVTPVSTEYFASSADAHLVLGQITPEDYTVPAPDSGEKTVDAALRRLARV
                     VCADLDEIGREGALEIARQFWEVQRTMIAAALERVQSRSGATGVITAGIGADLFAREL
                     GGVTLKAEIGDAADALPAYAVREVALR"
     gene            complement(49872..49994)
                     /locus_tag="PK196_00270"
     CDS             complement(49872..49994)
                     /locus_tag="PK196_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39890.1"
                     /translation="MNRKRKKNGNPNASAMSVMASDPASRSRLSPHRFTGAPPP"
     gene            complement(49987..50592)
                     /locus_tag="PK196_00275"
     CDS             complement(49987..50592)
                     /locus_tag="PK196_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39891.1"
                     /translation="MKKRLPVSPMLVDLVVNALDSMDGVAFTDPPSCPHCGGDVSGYD
                     MRAKRFAVVIEDGKRRTIRVMVKRFRCQDCGSLCGADAPFYPDTRLASPIVDLCVLLA
                     QQMPFNRVAQYLREMRLVVDRGTIRNYASREFEFGAIPATDVFGLQLPLSLLNLATLT
                     LNRKGGPIVGAEALAACGFPAADRTPLHPGRPPKERYQRYE"
     gene            50991..53057
                     /gene="fdhF"
                     /locus_tag="PK196_00280"
     CDS             50991..53057
                     /gene="fdhF"
                     /locus_tag="PK196_00280"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HON39892.1"
                     /translation="MDFKYVQTTCPYCGTGCSFNLVVRDGKVVGTQPYDRSPVNEGKV
                     CPKGTYAHEFVNSPDRLTKPLIKKDGKFVEATWDEAYDLIVEKFKACKPDEFAALASA
                     RVSNEENYLMMKFARGVMKSRHIDHCARLCHASTVAGLAASFGSGAMTNSILDIAESK
                     CVFVIGSNTFEQHPLIGRKIVQAKKNGAKVIYADPRYTPTARQADLYMPFISGSDVAI
                     LNGLMQEIIKNGWEDREFIEKRTKDFEKLKEVVMKEAYSLENVSKISGIPVESLKTAA
                     EWLGTNKPGAILYSMGITQHTVGVDNVRSVANIQMLLGNLGKPGAGVNALRGQNNVQG
                     ACDMGALPNVFTAYQKVIDEAAHKKFADAWGFPDGICEPKLGYEVTTMMNVLTDNPGE
                     LKCMYIMGENPMLSDPDINHVREALESLDFLVVQDIFMTETGELADVILPAACYAERD
                     GTQTSTERRVQRWRKAQDPPGEAKQDWQIICELAARMGYAEQFPFQSAEEIFEEIRRV
                     TPSYAGMTYERLDRPEALHWPCPTVEHPGTPILHREKFSSPDGLGVFAPIEWKPPAEV
                     PDEEYPFVFTTGRILWHWHTGTMTRRSATLDTEVPTGWIEINTEDAKALGIKNGERIR
                     AVTRRGSVEVPAKVTDDIMKGVMFMPFHFHECAANILTNNALDPIAKIPEFKACAVRV
                     EKITEA"
     gene            53060..>53720
                     /locus_tag="PK196_00285"
     CDS             53060..>53720
                     /locus_tag="PK196_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HON39893.1"
                     /translation="MAAKGDMFYAWAADAACQERGECGGAVTALLTHALRSGMVDAVL
                     AVKRGQDIYDAVPTLITDPEDMAETAGSLHCGTLLLSKLLKNYLDGAENMRIGITVKG
                     CDAMGLYELAKRNQVNLDNVLMIGVNCGGSVSPVTARKMIREKFGVDPDDVVKEEIDK
                     GQFIIQTKDGQHKGISMDELEDEGYGRRPNCRRCKMKVPRQADLACGNWGVIGDKAGK
                     AT"
BASE COUNT        11556 a        15788 c        15695 g        10481 t
ORIGIN      
        1 gagttcgtca tagatcttta tggtctcaaa gagcgcgttg acatcggaat cctcggggtc
       61 ggccagagcg agggaggtcg ccgcatggag gcacgcctcc ctgcccatga tggggccttt
      121 gatcccggct ttaaagccaa ggtcatcgtc gcgatcgaca cagaggacaa gcgtccgctc
      181 ttttgacatt cactagaaca tctttctgga actattaaat aattatcagg gcgccaaaaa
      241 atattagata agttttgcac gctgcaatag gagcaggtcg tcggttgtga tcttttcgcc
      301 gtcgcggaac tgctggaaga cgcgttctgc ctctttccgg acggctttct gctctcttgt
      361 gaccttggtc tttctggtct tcttccgcag accggatata accttatcgt aatcgcggag
      421 ttccttctgg caggcaataa acagcctgtg ctcctcgtcc gccgcctcct gagcctccac
      481 gaacttctgg tgggcgcggt cggcctcctc acgagatttg tcagccttcc ggtaggcctc
      541 aaccatcagg tcgtggtgct gctgtgcgag ttcagccttc tcggtgacct ccttatggat
      601 ctcggaggcc cgtctgcgga gttcccgtgc ttcggcaagc ttcgctcgca tctctttatt
      661 ctgctcaagt tcggcctcct gttccttcac cagcgctttg aggtgcttga tcttctcgat
      721 caactcgcgt tctttatcgg tgctgtagac ctcagtctgc tgcttgaact caagatgctc
      781 gatctgcttc tgcagttcct tgatgctccg gttctggagg ttgccatgct cctttttgaa
      841 ggcctcgatc tcctcaaaga gggcatttgc acgctcgtta tatccgtttc tctcctcttt
      901 cagcgcctgg acttccttat tgatcctgtc ccgctcctcc ttgtgtcgtt gcgcctcctc
      961 gacatattcg cgggtctgag cattcagttc atttcgttcc cgggcatact tgctggcaag
     1021 tgcgttcaac tcgttacgcc ggtttttgtg ctgttcagac tccgcaagga ttttttttcg
     1081 tttctcaatc agatcgttca gcattcctta ccaatcctcg acattcggta cctgtctcat
     1141 atccccaccc ggacgggacc gcttccatat tgcttcagga gcgccgatgc tcatgcagcg
     1201 gttacaccac aagaaaagac catttccgga ctcgcgaccc ggatcgtcgc tcatctgcct
     1261 gctcatgtgt tcaaccccgg taccctggca ccgcagaccc attgcggtct ctgccctgca
     1321 caacacaccc cgtgcggggg aggattatac aagaacgtct atacttcaaa cgtcatctct
     1381 catattaagt atttcgtagt gggcgaaaac gccgtcgccg cactcaacca acccgaatca
     1441 aaagagtatg aaagagggta agtgggaagt aatcacatga aatcaatgag gtgaccactt
     1501 gcggcatccc tcgccgcgcg cctgtttgat gaggtcagag gtcttgccga gacctgctcg
     1561 taggcggcgc gacgatttcc gaggatctgt gcacttttca caccggtcat gatcgccatg
     1621 acacggactc taccttcgaa gtcgctgttc acacgtgccc cccagatgac gtccgcgtgg
     1681 gggtcaagtt cgtaggtcag ggagcttgcg atctcctccg cgtcctggag ggtcaggtcg
     1741 ttgccgccgg ttatgtggat gaggctcccc gttgctccac ggtagtcgat gtccagcagc
     1801 gggtggttga gacactcatg aacgacgctc tcagccttgt tctgctgctt gctctcacca
     1861 acaagcatca cggcgacgcc gcccttgctc atgatggcgc gcacgtctgc gtaatcgatg
     1921 tttattagcg acggttccgt gatagtctcg ctgatgccct tcacggtctc cgcgatgagc
     1981 tggtccatca ccgagaacgc ctgaccgagc ggcaggttgg ggacgaactt gataaggcga
     2041 ttgttatcaa gcacgatcac ggagtcagca gtgttcgaga gcgcttcaag cccctcttcc
     2101 gcacggagaa gcctggcctt ctcaacctgg aaggggtaac tgaccatgcc aacgacgatc
     2161 gccccctggt cttttgcaat ctgcgcgatg accggggccg taccagtgcc ggtacctcca
     2221 cccatgccgg cggtgatgaa gaccaggtct gcgtcgcaga ggagggtctc cagtgtcggc
     2281 cgggccatct cagctgcgcg tctgccgacg tcagggaaac ccccggcccc aaggcccttg
     2341 gtgagcgact tcccgacgag aacccgcttg tcggcccgga tcatgtccag gtgctgcttg
     2401 tccgtgttga tcgcaatggt ctcggcaccg gtgacctgca tgtggtaaag gcggttgaca
     2461 gtgttgttgc ctgcaccacc gcacccaacg atgacgatgc gcggttggcc gagtatgtca
     2521 tcctcgacct cgtccgtctc ggatttcaga tatttttcac gctctgcgtg cttcaacgct
     2581 tcgttgatga tcgtttgcat caatatacct cctaaccgtt gaatgagatc tgatcggcct
     2641 cgcgcaggac acggtctcca tgcctctcga gaagtgctct gacagaatac cgcacagcct
     2701 ccgatactgt ggggaactcg ccagcctcca ccagcttctc gagcatctca acctgttgtc
     2761 ggggcaatct gatagtgatc cgatccatca ttccaatcac tcatatctga catatagcag
     2821 ctctaaagct gctaattgtc agacaagtgt aacctcttcg tctgactgta tcagataagt
     2881 cataataaca tttgtataaa atataaacct gtcgatatgt tttttcggcc gggataacgg
     2941 ttttatgggt tgcagacgac aaaatatctt caaaagagtt gccaccgata tgcgagagta
     3001 tcttcctgag agggcggagc atggcggccg ggtgcgctgg caccgcagcc gcgagatgag
     3061 ggatgaggtt cttgatttca gtgcgaacat gaacccctac cctccagata tccactggac
     3121 tcctgatctt tcagcactga aagactaccc agacgaccgg tatgaagtgc tcaaagaggt
     3181 gatcgccgga actttcgggc gcgacgccgc ggaggtcacc gtcgggaatg gatcggtcga
     3241 gctgatccgc gcgttctgtt gcgcgatgct cggacccggt gatgccgcct gtatcgagat
     3301 gcccacgttt gccgagtacg gggtggctgt gaggcttgcc ggagggagat gcgtcacagg
     3361 ggacgacggc gccaccgttc ggtttctctg caacccgaac aatcccacgg gaactctctt
     3421 ttcccgcgca gaagtcttcc gtgtgctcga tgcggtcgcc gagcgtgggg gttgcctctt
     3481 ccttgacgag gcgttcatcg aacttgccga cccgcgccag agtctggtcg atctaccaca
     3541 cgagaacctc ttcgtgctgc gatccctgac aaagagtttt gccgtccccg ggatccggtt
     3601 cggatacggg tttggcagcc ctgaacttat cgataggatt gagagggcgc gcctcccctg
     3661 gacggtgaat acgttcgccg agtcctttgc gatcgaggcc ttccgtcact acgatcgcct
     3721 ggaagagtca aggaagcgga ttgcagaaga acgggcctgg ctttctggga ggctggaggg
     3781 tctcggtctc gtctgcgtcc caccttcggc aaactacatc ctggtcggga tcccgctgga
     3841 gtcgggagtg ctcagcgaac ggctcctctc gcggggcatc ctggtgcggg actgccggtc
     3901 tttcgggctc ccccggcaca tccgtgtggc tgtacgaacc cgtgaagaga acaggcaact
     3961 catcgaggct cttgaagcat gcttgcactg atcatggccg ggggagaggg atccaggctc
     4021 gggatgggtg aaaaaccgct tgtaaacatc tgcgggcgac cgatggtctc ctatgtgatc
     4081 gacgcctttc ttgccgcaga ccatgaagtg gtcgttgtgg cctcgcaccg gacgccgttc
     4141 acaaaaaact ggtgccgggc gcagggcctc accatctacg acgcaaaggg gcttggctac
     4201 gttgaggata tcaatgccgc tgcggccgac cttgaggagg agggaaaacc cctcttcacc
     4261 tgcgtctccg atctgccatg cctcgcagtc gatatcatcg ccgatatcga ggccgcctgg
     4321 cgccgctcgg ggctgccggc atgctccaca tgggtgcccc gcgatctctg tctggaacat
     4381 ggctgccgca cacactacac agagatcatc gacggcgttc ccgcatgccc cgccggggtc
     4441 aacatactga cagccagccg cgaagagggg ataccagagg aggaggtgca ggttctcctg
     4501 catgaccggc gccttgcctt caacatcaac acacgcgaag aactggcact ggtgcagcgg
     4561 tatctctgcg gaaaacacga ggggtagcct ttttcaccgg tttcttgcct ccttttcgcg
     4621 gcattccgat cgtttccgat tctggcgctc accgggttac tgcgggacca gcaagtgaag
     4681 aaggagacgc accccgaaga gacactggga aaccctatta tcggactata accaacgttt
     4741 atatatgaga ccctgccggg aaatcgaatt agaggggcat atcatcgatt caggcatcat
     4801 gaccatggtt tttgacagga tcatggacat gggcggcgaa tttgagattc ttacgttcaa
     4861 cgtcgggaaa ctgaagaccg acaccagtta cgcccggttg cgcgtgaccg cccccaccga
     4921 acagcaactt gacgccatcc tctccgaact gcaccgtctc ggcgcacgca ccccggaggt
     4981 cagcgatgtc atccttgtac cggcagaggg tgaccgtatc ctcccgaagg ggttctattc
     5041 gaccaccaac cacccgacct acgtgaagta caaaggcgaa tggctgccgg tcgagaatat
     5101 cgagatggac tgccacatcg tgatcgatga aaaggagaat cgtgcgatct gcacccccat
     5161 atcaaagatc agggcggggg atctcgtcgt ggtcagcgag accggtgtca gggtgatcta
     5221 tcccgagcgc ccccggaagt ccagcacgtt tgagttcatg cacggaacgg tatcctctga
     5281 acgccctagc aaggcgatca taacaaagat agcccgcgag atcctgaaac tgaagaagga
     5341 gggcgggaag atcgcgcttg tcggcggtcc tgccatcgtc cataccggag cggccgacgc
     5401 cctcgcaacc atgatccgcg atggttatat cgatgtcctg tttgcgggga acgcgcttgc
     5461 cacccacgat atagaatcaa acctctacgg cacgtcgctc gggatggaca tcaggacggg
     5521 cacgcttgtc accggcgggc acaagcacca catccgcgcc atcagcgaga tcatgcgggc
     5581 aggctccata aagaacgcgg tcgaccgggg gatcatcacc ggcgggatca tgtatgagtg
     5641 cgtgaagaac gaggtcccgt ttgtgcttgc cggctccatc cgcgacgacg gtccgcttcc
     5701 ggatgtgatc acggacgttg tggaggcgca ggacgccatg cgccggcata tccatggtct
     5761 ctcgatggtg gtgatggttg gaacactcct ccactcgatt gccgtcggga actgcctccc
     5821 ctcatacgtc aagaccgtct gcgtcgatat caaccccgca tcggtgacga aacttatgga
     5881 ccggggcacg acgcaggcga ttggcgtcgt gagcgacgca gggacgttcc tcccgttgct
     5941 ctgcgagcaa cttgaagaac tggagaggag tcagcgggta agcggcatgc agtagggctc
     6001 ttcctgctca agcacaaatt cccactcttc tttgcagccg cactccatct ctgcaagagc
     6061 gcgcaggaga ccggcgtcgt tgttcagcga gtagtcagca atccccttca ctatcgggct
     6121 atcgcaggcg ccacagttgt gcgggccgcg catctgcccg ccaccgacgg gatcgcagaa
     6181 aacatgcacc ggcgactgaa tcaggacgtc caggacgctc cagaggtatg gcggccggta
     6241 ggcgtgctct ctccagaggc gctcgacttc ggtcctgctc tggacagtgc agaggttcat
     6301 ggagatgctc tcgaccaggg gggcgagatc cctgatggac cggatcatgt catcacgcgc
     6361 ctcgcgctcc gtcaggaacg gcggtttcat cagcagatag gccttcacgc ccgcgccggc
     6421 ggcgtgcgca gtctctgcgg cgcggaggaa atcggcgtag gtgaacccct tgtctatgca
     6481 cttctcccgg atcagatcgt ctgtggtctc aagacccatg gcgattgtga gcggcatggc
     6541 ccgggtgccg gtatcgatct cctcgcggaa ctcgcgaacc ccatcggcct cgacgtattc
     6601 cggccgtgtc tcgactatca aggccttgcc gcggaacgcc tctgcaacag cgtgccgggc
     6661 ggccggtgga acctccctgg catcaaagaa actgccggag gtgaatacct ttaccaactg
     6721 gtaatcgtcg tcggagaagt tctctttcac ccagcgaacc tggcggatga tccgttcggc
     6781 aagttcggcc ccggcgagat cctggtaacg ctcgtgcctg tagccgcaca tccggcagcg
     6841 gttccaggag caacctccgc ttcgaaagat caccgtcagc gtatccagga tccggccctc
     6901 gtgccggtct ttcccgcgcc aggaggccag cggtttctcg gttaaatggg atagcattat
     6961 acctcatatc cgttgatcta atggtatgaa aaggatcgca ttgctggcgc tcatcgccgc
     7021 ggtggtgcta atagcacctt catcggccgc atatgtctcc atcagcgcac cccaggagat
     7081 ttacgtcggc gacccgctcg ttgtcgtggg tacctcgacg atagaggggc agccgaaacc
     7141 ctcgctcagc cccgggttca gtaccgagat cgtcctttat tacgctcagt acgccaagcg
     7201 cgagatcgag cggaagacga tcgtcgtcca ggaagacggt acgttcaccg cgacctttga
     7261 gactgagggg ctcgaaccgg gacgctactc gatcgagatc atcgacccca cgaagaccac
     7321 gtttggaggc agttcaaaga cgcagcagct ggttaccctg atcgaccggt cggggatgat
     7381 cacaatagac tcccccctca cccaggagtt tgacggcacc cttgatatcc ggggttccat
     7441 cgctgacctg ggggatgcgg gggtgcaggt cagggttgag catgatggct tcatagtcta
     7501 cggacctacc tatcttgcaa ccgacaaaaa tggggcgttc tcggcggagg tgccaatctc
     7561 tgccgggggc acctacgagg tcacctttta cgacgccagg ggctacatcg gcagccgtga
     7621 gtttgttgtg gttggtgcac cggaaccaac ggcaaccggg gtgatcatct ccgcaaccgc
     7681 tccagccagc cgttctgctc ccgcctactt tgaggttgag acccggagcg ggagtgtgtc
     7741 gatcacgacc tcgtccggga tcgactgggt tatcgagtac atcgatgaga agaatgtgcg
     7801 ccataaggtc aacgagaaag gcatgcttga gccagaaaag gtggagatcg ctgccggggg
     7861 cggcacgatc tacctgaagg tttatccgct gagttacagc gataacacaa cggtgcagat
     7921 ctttgccgcg aatgccgagg cggtcagggt gagccgggcg gcgccaggcc tcttcgggga
     7981 cgcaacccca acacccacac agtcaacgcc gctcccgccg atccttgcac tcctcgccat
     8041 ttttgttgtc gtcctcgcca ggaggggatg aacaaccttt ttttgtgcgc tgcccaatag
     8101 gttaaggcag gccgggatag tctagtccgg taaggcggcg gccttgaaag ccgctggtgc
     8161 tctgcacctc gggagttcaa atctccctcc cggcgctgat ttaagatccc accatttcgg
     8221 gtgggtggtt tacagaatct tctctcctgc ccttgacccg ggcgtgcact cgtagacctc
     8281 ccggatcttc atgatgagca gggtctttgc cggtgccttt gccatcttcg accggacggt
     8341 aaactgcatc ttttcaaact ccgggccgct cgtcctgatc tcgacatcgc cctttatctg
     8401 gaagcagccc tttgtctccg gtccccagat gtagatggca accctggggt tctcctggac
     8461 gtttgcaagc gtcttcttca tgtagttgtc cgctatccag atagtctcat catcgacaat
     8521 ctccacaaac gcgatcggta ccacgttcgg ccatccctct tttgacgcag tggcgacggg
     8581 ataggccttc attgtgcgaa aagccgcttt catttcttca gttagtgcaa ccatcgtctc
     8641 tcacaggtat acactctcga ctacacatat ataaataaga tccatcctga gatggggggg
     8701 cagaggatgg ccatcaaacc ggtggactcc tgatatcctc aaatacgccc tgtgaaaggg
     8761 gcgggcaagt gtctggcgat tgtcccggaa gccgtgcacg gggacgagga tctcggtgag
     8821 ggtgatcaca ttgacggcgg tgggatgctc cctcgtggca ttctatcgat ggcaaactgc
     8881 aaatcaagag agggccgatg gaggggcggg aaaggatttg ccatcagacc cggacaaaat
     8941 ttgggagcga ggcgtggaga ctctcgttca ccgacccccc tatctccgga ataacgcctc
     9001 acaaaaccct gcttcgcacc cacccgtcgc ggggtagttc accgatggag cagggaataa
     9061 gagaaaaacc gcaccatatc agggatcgcc ggccgccaga gacaccgtct tttcgcagca
     9121 gagcgtctcc cggacctccc ggatgggaca gcggtcgcag agcggcgact tcctgcagat
     9181 ggtctttccc aggtgcacga tctgggcatg gtaatcgttg aagagctcca catcgggctc
     9241 caggttctcc gcgaaaagac gttgcgtccg atcgtaggat gcgtcttccg gcagcagacc
     9301 atagcgcgag aatacccgcc gggtataggc gtcgacaaca aagaccggtt tttcgcaggc
     9361 atacagaagg atggtatcca ccgtctcctt cccgagaccg cggatggcaa gcaggcgcct
     9421 gcgcagtaga tcagtatcaa cggccgccat cgcccccggg tctgccgcga actctgcaac
     9481 gtagacccgg gagaactccc ggatccgttc cgccttctgg ttgtagtagc gggatgggac
     9541 gatcaggcgg gcgatctcag aggtctctgc cgcggcaagg gcgtgggggt caagcaaccc
     9601 cgcctcatcc aggttccgaa cggcctgcgc cgcgttcgtc caggagacgt tctgggtgag
     9661 gatggccccg atcatagtct caaaaggtgt ctctgcgggc caccagtgct ggtggccaaa
     9721 cgccgaacgg agggcgttgt agataacaag gagatcatca gtcaggttaa ttctcatcat
     9781 agccaccacc gtcctgcagc gatcggcatc tgccagtccg tcccgaacgc ccggccggtc
     9841 acacggatcc cgggcggggc ctgccggcgc ttgaactccg ccagttcaac catcctgaca
     9901 accttccgga cggtctctgg cgggtagccg gcagcgatga tatcatcagg cgactcgagt
     9961 cgatcgatcc agcgctccag gatcgcatcc aggaggtcgt aaggcggaag ggtctcctga
    10021 tccacctgcc cgggccggag ttccgcagaa ggcggcttct cgaggacgcg ctgcgggatg
    10081 accggacgcc gggcgttcag ccagcgggca acccggtaaa ccatcccctt cgggagatcg
    10141 gcgataacgg caagcccgcc tgccatgtcg ccatagagtg tgcagtaacc caccgcgatc
    10201 tccgatttgt tcccggtcgc aaggagcatt gaaccaaact tgttcgcaag ggccatcaga
    10261 accgtcgccc tgatcctggc ctggaggttc tcctcggtgg tgtccggggg catgccggag
    10321 aatacaccgg caagcctgga agagaaagtc tccatcatcg gggttatcgg gatgcagcat
    10381 ctccggattc ccaggttatc cgcaagttgt tctgcatcct ctatgttagc agcggaggtg
    10441 tagggagagg gtaaaagcac ccccaggacg ttctccggcc cgagcgcact ggctgcgacg
    10501 gcggccacaa gcgatgagtc gatcccgccg gagatgccta tgatgaccga tctaaacccg
    10561 catttccgga catagtcccg tgtaccgaga accagagcac gccagatctc tgattcaggc
    10621 agatactcgt cggcggcgac agcctgcggc gcagggttgt cgagatccgc ggtcacgatg
    10681 tcggcatcaa acggcgcacc gcgggcaacg agcgttcccc gggcgttaaa gacgacgctc
    10741 cggccgtcaa agaccaggtc gtcgtttccc cccacagagt ttacagacgc gatcgcgacc
    10801 ccgtgctcac gggcaaacct cgataacgcc tcctcgcgca ggcgccctct ccctgcggca
    10861 aacggcgatg cgaccgggtt aacgatcacg tccggcatcg ggccctcgta gcagatactc
    10921 tcaccgatga ccacgccgag ggtctttccg ccaaagcgga tgaacgggat ctctccgccc
    10981 gcaggctcaa agtaacgatc ctcatccagg acgccgggag agatggaggg ttttcggaat
    11041 atctctgcga cctccccctc cctgataaga gcggcggcgt tgaaggccgg gcggcccgtg
    11101 ccggctggat tcgctatcgg gaggccgatg atgaccggcg gtgcaccggc gaggcttgca
    11161 gcaagttcat ccagtgcaga gagggctcgc gcagcaaacc ctggccgcag cagcagatcc
    11221 cgaagggggc accccgtcag ggagagttcc ggtgcgacga taacgtccgg tcggtcccgt
    11281 gccgcttccc gggcacctgc cgctatccgc ccggcgttcc ctgcaagatc gccgacaacg
    11341 gtattcacct ggagtagtga gaccttcatg acagctcccg gctacatctg atcagtaatg
    11401 gcagaacaaa cggcatatgt ctttggcctc gatccagcga aacggcatgt gcggccgccg
    11461 cacccatcgc ccacaccgat cagatgcctt ttatgcgcga agatcatagc gccctgtgag
    11521 aacatgcaga gagcatacca tggaatgatg ttgaggattc tccgggggtg ggcgggctga
    11581 actcccgccg ccattggaga actggaggag agacacatcg tctcctcctg ctcgctgtat
    11641 cggttattgt cgtctttgcc gtaaacgttg ccggccttct ttcaggcatc tttaccgtcc
    11701 tcccgcacct cttctacctg ccgatagtcc tcggtgccta ctggtacccc cgccgtggcg
    11761 tcccattatc gtttggcctg gccgccggat acctggcaac aaccatcttt tttgccggtc
    11821 tggatatggg gctgctcatc cagactcttg cacgcgcggt ggtgtttgtc gccataggcg
    11881 ccgtcatatc actcctctcg ctccggttgc gggagcagga agagcgctac cgcggcgtct
    11941 ttgataactc tgaggccgga acgtttatcg ttgcatccgg ggttgacggg ccgcgcatcg
    12001 aggaggtcaa ctacgttggc gcctcgctcc ttggctccac tatagatgac ctgaccggaa
    12061 aacccataac cctgttcttt ggcgatcccg ctctctggga gcgcctggag gcagatagcg
    12121 accggagagg cgctgtctac gggtttgaga caaccctgcg ccgaaccgat ggggagacca
    12181 tacgtgcgct tgtttctgca ggccgactgc agggagggcg gatcgtcctc acgctcatcg
    12241 atataacagc ccttaagacc gccgaggaga cgctccgccg gacaaacgcc aaacttaaca
    12301 tgctcgggag actcactcac agggatcttg ctgcagcggt ctcgcaactc ctggcgcaga
    12361 tcacgcaggg aatgcggcat tttgacgatc ctgcgtttcg ccgctacctt gaggagctgg
    12421 aggagagcgc ccgggttgtt cagcgccggg tggagatcac ccgcgactac caggatcttg
    12481 gtctccagcc gccagactgg caaccggtgc aggaggtgat ccggcgggcg atgtcgtgtc
    12541 tgctgatacc gcccgaggta tccgtccgct gctgggtgga acggcttgag gtcttcgccg
    12601 accccatgct cgaccgtgtc ttctcaaacc tgatcgagaa cgccatccgc cacggaaaga
    12661 cggtatcctc ggttgtcatc acctaccaga tcctggatga cgggcttgca atctacgttg
    12721 aggacgatgg cgtgggcgtt cccccggaaa agaaagagcg tatatttgat tatggcgtcg
    12781 gcattgatgg gggtctaggt ctctttctgg tcagggagat cctcgccatc acaggtataa
    12841 cgatccggga gacgggaaca cccggtgagg gggcaaggtt tgtgatccac gtgccgccgg
    12901 gcggatacag gataacctga aaggaggaga agatgtttcc aaaagtgctc atccctacag
    12961 acctctctgc ggcctccgcc accgttgtgg aaaggatcgc cgaggtccct ggcgttcgcg
    13021 aggtcatcct cttccatgcc ctgaggtcat cgaccggcct ctcgcctgcc gatgaggaga
    13081 cgcttcagcg gatgcaggaa accgtgcagc gccaggggta ccctgccgtt gtggtggtgg
    13141 aagatggtat ggcgggcgtc ccggcacgga tcctccagac tgccacggag agggacgtgg
    13201 acctgatcgc catgggcgtt cgcgatccgg ggctgctccg aaacctcttc tccggaaacg
    13261 ttgcggctac cgtgctcagg gatgcccatg tccatgtcct gatcctcccg cagtcggcga
    13321 gaagggggcc gcccctgggg gcgcggctgc tggtgccgac cgacctttct gacccggcgc
    13381 ctgagatccg ttcgctcctg cgggaatctg atggggaggg gatggcggtc ctcctccatg
    13441 ttatagaacc ggggcggaca gagcaggagg cgcgcgacca gctcgaggcg ctaagggcag
    13501 aactcgcacc cctgcggcgc gatgtcgagg tgatggcacg cacaggcgat ccggcgcgca
    13561 caatctgtgc cgtggccgag gagatcgagg cctccatggt tgttatcccg cgcataggca
    13621 gacgtgacgc agcaggggcg gccacgctcg ggagtgtcac tggagctgtt atagggtgcg
    13681 taaaaagacc gttgctcgta atcgccatct caatacacgt ccagatcgag acccgggaac
    13741 tgaggagcga agagtttggg cttgctgagg agatctggga agactatcat gggttgaagg
    13801 ccgatccgaa gacggatcgt atattcgggg tctttgccga cggcaggctg gtctcggtcg
    13861 ccaggtgccg ccggcacccg gacggctacg aggtagacgg tgtattcacg ccggtaaggt
    13921 tccggggcag gggttatgcg cggcgcgcga tggacgcgct cgtcgaggcc tgtcagcatg
    13981 acaccctcta catgcacgcg gtcaggaatc ttgttgactt ctatgggaag tacgggtttg
    14041 tctcgatacc ggagagtgag ctcccgccga ccatcagggg gaggtacgcg tttgctctgg
    14101 gcgagatgga gggggcaaac gtccagccga tgcgccgtcc acccgtccgg ttcatgcggt
    14161 aagcaggacg aaccggagag gctgcctccc tcccctcccc ccctgcacct caccggaaga
    14221 tcctcctcac ccacagaaca gaacaggcaa ccatataata tcacgtgaga gcaccagggg
    14281 atacataatg ctaataggaa tactcgccga cacccacgac aaccttgaga aggtggatgc
    14341 ggccgtaaga tctctgaacg cgaaaggagt ggagatggtg ctccacgccg gggattacgt
    14401 ctccccgttt gtcattccgc ggtttgccag cctgcagtcg ccgatgatcg gcgtcctcgg
    14461 gaacaacgat ggcgaccata gactccttca gagacggttt gaagagcacg accgattcag
    14521 cctgcgggga cgtttcgcct gcatcagcgc cgatggactg gcaatagggc tgcttcatgg
    14581 cgatgaccac gaactcctcc aggcgctcat cacgagaaag gtgtttgatg tcgtcgtata
    14641 cggtcatacc catcaggcag aggtcaggag tctcccgggg acgcttgtca tcaacccggg
    14701 ggaggcctgc ggttacctga ccggccggtc gaccgttgcc gtgctggaca ccgtgacccg
    14761 gagcgtcgaa ctgctcacac ccggacacga agagctgtag aagggtggtg tatcccggca
    14821 ggaggcaaag acgcggctgt agcggcagca gcaagaacat atatgtggtc tcgcaggtta
    14881 ccgccataag agggctacac accatggaga tcgttaagat acccaacatg gacagggcgg
    14941 agtatgacaa actcatcgag gaggggtttc tcagccgtat cgcatttcag ggcgccaaat
    15001 acccctatat cgccccattc ctgtatgtct ttgatggaaa gtttctatat ttcctggcaa
    15061 ccaaatacgg caggaagaac aaccttttcc ggcagcaccc gtacgtatct gtcgagatcg
    15121 agaagtattc aagcgacctc tcctgctaca ctttcgtgac gatgcagggc tacctggttc
    15181 aggtcgagga tgcgatcgag aagaagatca tccgggagaa gttcgtggag atgatcggaa
    15241 aacacaacct ctcacgcaac attcttgcgg cgctcggcca catgcccgag gagccgatcg
    15301 aggcgatcgc ctccgaggag cgctcaaaca tatggaaact gaccggcgtg aaggatatag
    15361 ttgccttaaa aaacctctga gaccattggt tgagggagcg cgaaatgccc cccgacgcgt
    15421 tcacttcacc gacccctcca tcatcgtccc caggcgctat aaacccctgc gcgcaacaag
    15481 tttctgtatg gaggatttca cggcgtgatc cacataagcg aaggctggga cgaccttgcc
    15541 gcagcgctca tagaatcggg tctcagagag agcgtctaca tcatgggggc gacggacacc
    15601 ggaaagacaa cgctctgccg ctaccttctc agtcggacgt cagcacacgt gcggacgggc
    15661 tgcgtcgact gtgacacagg gcaatcctgg atcggcccgc cgacaaccga ggggatgacc
    15721 atccaccctc gtccctcaaa acccgttcta cggtttgtcg gttcgacctc accccggggc
    15781 cacctcgtcc agacactcac cgcggcaaaa cgcctcgccg agtgggcctt gaaagacggt
    15841 gcccgcataa ccctgatcga ctcccccggg cttgtccatg gcggggtggg catcgagttc
    15901 cagatccaga tgatcgacct cctccgcccc gatcatatcg ttgctctcca gcgcggccgg
    15961 gaacttgaag gggtgcttgc aaacttcgcg cgccacccca ccatccagac tcaccggttc
    16021 cccgtctcca ccgcggtcct cccgcggacg gcagccgaac ggcggcgtta ccgcgaagag
    16081 cggttcaccg cctattttgc cggcgcgcag gtgcaggaga tcgcgctccg cgagccgggg
    16141 ttacagggga gggtgcccga tccggagaac cgatcggggg ttgccggacg gctggtctcc
    16201 ctcaacgacc cggagaactt tgtcatagtc ctagggatcg tcagcgagtt cgacccaaaa
    16261 gggcgcactc ttacagtctt cgccccgcca ttcgacacaa cctccgtcgc atccgttcgg
    16321 ttcggttcag tttcccttga cctcgaggcg ccgcctggaa cgatggagtg tcacaggttt
    16381 gaggcctcta cgcagggaca aacgagagcc agaccgcata caccaccgcc tgggctattg
    16441 tgagcgggac gccgacccgc gcgaacctgg caaacgtcag cgtctcacct tcacgctccg
    16501 ccccctggat tatgatgacg ttgcttgccg cccccaggat cagcatgttt ccggcgatgg
    16561 tgctgccggc cgcaagcgcc atcatcccca cggtggacgc tcccgcatgg gagagaaccg
    16621 ggaggaaaag ggcgacaaac gggacgttcg atatgaactg agatactatg acgcccagcg
    16681 cgatgatgac cgacacagcg cccggatcga gcgacctccc ctcaaccaat ggctgaaaga
    16741 cgcccgactc ccagacgctc gccgtaagga cgaacagagc agcgaagaag accaaagtcg
    16801 gccagtcgat ccggcggagg atctcaaacc ggcgcctgct ccctataagt atggggatgg
    16861 ccgcaaccgc cgcgatccag gtcagaggga tctcaacgtc cgggacgagc atgacgacag
    16921 cgaccctgac gacgatcatg atcacgagca ggacgagcga aagacgggta agggccgcaa
    16981 gaaccgggtc atggatctcc tcctcacagt gggcgagttg acctgctgat ttaaattcat
    17041 cggggaacgc ctggcggaga aaccagtatg taagcaggag tgagatcacc gtagggatgc
    17101 caagatagag cggaaaagtg atgaacgggt ttgtaacacc gcccgagagc gcgatgagga
    17161 ggttctgcgg gttcccgatg gggcttgcaa cactcccggt tgttatggcg aaagcaagcg
    17221 tgagcaacag gagttccgct gatataccgt gccgccgtgc gaagtagagc atcagcgggg
    17281 tgccaacgac cgccagggtg tcgttcatca ggagcgccga gaggcccccc gcgccgatga
    17341 ggatgaggaa gaccaggctt ccgactgatt ccgcccgaca gaagaggcga acggagaggt
    17401 ggtagaggta accgctcgtc gcgagcgcct caccgagaat gaacataaaa aagaggaaga
    17461 gcatcacatc ggtgttgatg gatgcaagcg catcgcccgg cgtgatcgat cccgtcagca
    17521 ctaccacaac cgcgccaatg agcatcacct gccagatccg gaggtcgacg ctcccgatcc
    17581 tccggacagc gatcaggatg aagacgatga tgagaacgat aagcggtata aatgtgccca
    17641 tggtatcatc gggagatgag aactccgcgc tatcgatagg tttgcgtgcg gccctgaaga
    17701 aggactgctc cgaggtcact ccaccatgat cagggtaatc cagagcagaa gggcaaacag
    17761 cagcacgatc agggcggccg accgcttcat gtaacgctga gcaatcgccc ggtcgcggct
    17821 gctccttggc ctgaccttca gcatggccac agccagaagc gcaccgacga tcagcccgac
    17881 aacctgcccg ccaaccagga gcgcagaggc tatcaggctc ggcggctgga tcacaagaac
    17941 gatacccatc acggccatcg gcggtatgat ggcggcagcg atggcaacac cggcaatcac
    18001 ctccgaagtg ccgcgattga acgccagcac cgccgcaaac ccgaggagaa ccgccatgag
    18061 ggtgtatatt gggttcacca caacccgtga agcgatctct gcagtcaggg tgaggggtat
    18121 gacgaggttt atcaggaatg cggcaactgc agagagggca aagacgctca ggagcaacgc
    18181 cgcgaggact ccaaggcatc gcagagactc ccggaccata ccgagagcag catagatgct
    18241 gaagccgtag atcgggccta tgatcgggga gagcagcatc gccccgatga tgatcacagg
    18301 gttgttgagg agcagcccgg agagcgcgat cagaccggca atggtggtca gcgccagttt
    18361 ctcaaggtcg agttccccat aaggccttat actgtcgagc aatttctcga ccggtgtctt
    18421 ctcctctttc ttctctatgt tcttcacggc gcgcttgagg cgggaggaga tgacaaactc
    18481 gggtgtggac acctcgatca ggttctcgtt gtagcggaga tcgagaacgt tctggatctt
    18541 ttcaatcaga tcatccaggt gctcatcggg cgcgtatatg gtgaacacgt agacatcctg
    18601 gaggaggatc tcggtgtagt gaaccccctc cagagcttcc tttactttct catagccgtc
    18661 ttttcgtacg tggacgacaa tcttcttcat acgcctgctc cactgatcca aatctaccga
    18721 attggagcga atcccctgaa attgatccta taaccggtca attgaccaga tacgtgggat
    18781 ttatgcattg ttagccgatc aagcccgatc tcggtagcac gagaccatcg cacgcttatt
    18841 taaggcgaaa gacctcccca tttttacccg taacctttgc agggcctttt agccacggat
    18901 cggtgcgatc ccggcaacac cactctgtat gacgggtaca atgatcgcgg atgatgttaa
    18961 tccttcgcgg ttgtgcttaa gggatgatgc ggggaggccg tcagatcaac gatcggaggt
    19021 gtgaacacgc cacattttcc cggtaatacg catcgggatt atcaaatccg ctcacctata
    19081 aacgttcctc gtcgaccacc ccccctttcc ggccgttcac gcgggctttc gggacctccc
    19141 gttcactcca tttaaggttt acgctgcgat cgcccttctt cgcaccccgg ggtcactcag
    19201 ggttgacctc gatgctctcc cccggcgaga gtaacgcgac ccggatcggc gtcgtccgct
    19261 cgatcgtctt cttgaactca tccaggttct gctggattgc agggaaggtg ttgtagtgca
    19321 tcgggatcac cagtctcgcc ccgatgtagc gcgccgcgat catcgcctcc tccggcccca
    19381 tcgtgtagca cccccccaca ggaaggagcg ccacatccgg tcggtagagg tcgcggataa
    19441 gctgcatatc cgagaagagc gcagtatccc cggcgtggta gacgctgatc ccgtccatcg
    19501 tgatgacgaa acccgcgggt gcaccgccat agaacccggg ggcgtcacca tccagtccgg
    19561 atgagtggag cgcaggcgtc atcgtaaaac ggacaccatc gacggtgatg ctgcccccga
    19621 tgttcatcgg ttcggcagga acgcctttag tcgtcaggta gtgcgcaagt tcagcgatgg
    19681 caaccgtctt tttcttcaac cgcactgcga tgcccaggtg atctgcatgg gcatgcgtca
    19741 cggcaaccat gtcaggttcc gtgcggagcg cgccttccgg catgaaggga tcgatgagga
    19801 cacgctttga tccttccagc agaaaacacg catgaccaac ccagtttaac ctcatgaaga
    19861 agagatcgat ccggagacta aaaaaggttc agtgaagagg gcagggggtc agaactcacc
    19921 catgtcgatc gcttcggtga tatcgatcgc ctcgctcagg ccatcgttcg cgacaccgcg
    19981 cgtcatcgcc tcggaaagat cccggtcctc catcacatcc ctgatccgtt ccaggtaggg
    20041 atcgagcgtg gtatcgctcc tctgctcgat cacgtggcgg tactcacgga gagtcgatgg
    20101 gcttacattg agctcctcgc tgatctcttt cagagtcttg ccggactcaa ggagtttcag
    20161 caactcctcc tggtcaaatg gcatcttgaa gtcaaggtcc ctgaagagtt tgaggcggac
    20221 cctggcacgt gccacggtct tgctcaattt ctcgtcgccc agcgccctgg ctatctcggt
    20281 gtcgttcata ccgtcgtagt acgcaaccac aagttttgcg agctgccgcg gggtgagttt
    20341 ggtcttaaaa taaccccgat cgagtatctc acgcatgatc agggcaatct cgcgctcaac
    20401 agcatcacga tcccgcagtg ttccatgaac ctttctcatc ggttcgacga ttgctttctt
    20461 gtttgttatt gttgtaaaaa gctccaggag ctgccgcttg cgggtgtctt ctgccatgtc
    20521 catctcccta acttctgtaa atccgctgtc cggtcaatga cgttacccat gataacccgg
    20581 atatatactt tgtcccaccc ctctgggtgc aatgttccct acattacaac ctttagcatt
    20641 taaaacttgt catatacccc tcaaaacggg agattttcta ctgtatgtag cccgcccgga
    20701 ctcacgatcg gatctgtcca ggcaaatgga aacacagatg ttcgtccccc tccctgcctc
    20761 tgtgagaaca cgtctcaggt gctggaaagc ccattcttta ttagcaccac catgcaactt
    20821 tactgtaaga ctatggccga gacaagcgat atttacgaga aagtcatgga tgttgccagg
    20881 agacgcggtt ttgtctggcc gtcatccgag atctacgggt cggtcgccgg gtttatcgat
    20941 tacggcccgc tcggggcgat gctgaagaga aacatcgaga acctctggcg ctcattctac
    21001 gttttccagg aaggctacta tgagatcgag tgtcccacga ttggaacaga ggccatcttt
    21061 gtcgcatccg gccatgtgaa agagttcgcc gacaagatgg ttcagtgtcc gcactgcggc
    21121 gagtacctcc gcgctgatca cattgcgcag gagcacggga tcgagaacgc cggcaccctt
    21181 tcggcggaag cccttcaggc agcgctccgc ggcctgccat gcccctcctg caatgaggtc
    21241 ctgggcgagg caggcgtctt tgcgttcaac ctcatgttcc agacgaccat cggcccgggg
    21301 tcgcagagga agggttacct ccggccggag accgcccagg gcatattcac cgattttccc
    21361 cggctcctcc gtttctaccg ggacaaactt ccttttggcg ccgtccagat cgggaaatcc
    21421 taccgcaacg agatctcacc ccgccagggc atgatccggc tgcgagagtt ctcccaggcg
    21481 gaggccgaga tctttgtcca ccccgacgag aagaagcatc cctcattttc ccgatacgcg
    21541 gattatgccg tgccgctcct caccatcgca gggcaggtgg agaaccgcga acctgtcacg
    21601 atgactatgc atgctgctgt ggacgatggg ctgattgcaa acgagtatgt cgcctactac
    21661 atcgcgctga cacaccagat cctgacctct atcggtatcg atcccgcgaa ggtcaggttc
    21721 cgccagcacc tgacagagga acgggcgcac tacgcggccg actgctggga tgccgaggtc
    21781 tattccgaac gtttcggctg ggttgaggtt gtagggatcg ccgaccgcac cgactacgac
    21841 ctgcgctccc acgcggagca ctccggcaca tcgatgacaa tcttcgtccc ctacgacgag
    21901 ccccgtctgg tcaaaaagcg ccggatagtg cctgacatgg gcgtgatcgg ccccaggttc
    21961 cgcgagaagg caaaagccat tgtggaggcc ctggcggtct cggagccggg agaggacggc
    22021 gcgcggataa ccctcgacgg tgaggagatc ttcatacccg ccgacctcta ccaggtccgc
    22081 gaagagatgg tggaggtcag gggtgaagat gtgatgcccc atgtcatcga accttcatac
    22141 ggcatcgacc ggatgatcta tgccgccctc gagcatagtt acgccgagga tgaggtcgat
    22201 ggcgagatcc ggcgtgtgtt tcgattcccg ccggcggttg cgcccatcca ggttgcggtc
    22261 ttccccctga tgaaccgcga tggccttgat acaatcgccc gcaggatcgc agaggccctg
    22321 ctgcagcacc gcatccttgc ccagtatgat gattccggtg caatcgggag gcgttacagg
    22381 agacaggatg aggtcgggac gccttttgcc gtcacagtcg atcacgatac cctggaggac
    22441 aacactgtaa ccattcgaga ccgagacagc atggagcagg tcagggtgac gatcgagcgg
    22501 ttgcccggga cactctctgg cctcatcgcc ggcaagatta agttccacga actcaaggca
    22561 tgaaatacgt ctcccacccg ctgatccggc cacagagcat cgaggagcgg cgctaccaga
    22621 tctcaatcgc cctgagggcg ctggatatga acacaatggt cgtcctcccg acgggactcg
    22681 ggaagaccgc cgtagcgctc atagttgcag cgtcccgact ctactcccac caggggaagg
    22741 tgctcgtgct tgcgccaaca aaacctctgg tcgagcagca cctccggttc ttcaggcagc
    22801 acctgctctt atcagaaggc accgaaccgg aggagacgga ttttgctatg ttcaccgggg
    22861 ccaccccccc ggacgaacgg gcacaggcct gggagaagtg ccggatctgt tttgccacgc
    22921 cgcaggtgat caagaacgac tgtctggcag ggaggtacag cctcaccgat gtcgtgctgc
    22981 tggtcgtgga cgagtgccat cgggcggtcg ggaactacgc ttacgtcttc ctggccgatc
    23041 actactgcaa cacggcgaag aacccgctgg ttcttgcgat gaccgcttcc cccgggggtg
    23101 acccgctgaa ggtgcaggag gtctgcaaca acctgcacat cgaggcggtc gagacacggg
    23161 tggagaccga tgaagatgtc ctcccctaca tccacgagcg cgagatacac tacatagacg
    23221 tctacctgcc ccccgaactc caggaggcgc tcgtgacact gaaggggctt gtggaatcac
    23281 ggcttgaacg acttgcgaaa ctcaattacc aggttccaaa agccgataaa ctctcaataa
    23341 aggccttaaa cgccctgaat gcccagatcc agcagcgcat acaggcacgc gatccctccg
    23401 ggttcatggc ggcatcgctg catgcggagt gcatgaaact tcgccacgcc atatcgcttg
    23461 ccgagaccca ggggagcgag gcgctcaaac tctaccttgc aagactcggt gctgagggtg
    23521 cctcgagcgc cgggagcaag gcaagcaaac ggcttgtctc ggatccggca taccggaggc
    23581 tggtcgagac cgcagcgggt tggagggagg agctccaccc gaaggtctcg atcgtccgcg
    23641 aactggtgga ggcccagctg gcagcgcacc cggagagccg gatcatcgtt tttgccacct
    23701 accgcgacac cgtccagacc ctggtcgata cgctcagggc ggctggcatc ccctgcgaac
    23761 ggttcgtcgg ccaggcgtcc agggacgcgg agcgtggact ctcgcagaag gagcagatcg
    23821 caaccctggc ccggttccgg gcaggggaac tgaaatgcct gattgcaaca tccgtcggtg
    23881 aggagggact cgacgttcca tcgaccgata tggtgatctt ttacgaggcc gtcccctcgg
    23941 agatccggag catccagcga aagggccgga cagggcggag cggcagcggc acaatcatcg
    24001 tcctggtgac gaaaggaacc tccgatgaga cgttccggta tgtgagccag aacagagaac
    24061 gggcgatgat gaggggcata aggaaaatga gtgccgctcc tccccataac cctgccaccc
    24121 gacagacgga gatcctggcg ttcgcatcgc cggggccggc gataaccgtc gatgacaggg
    24181 aaacctcatc ccgggttgtc gagcgcctgc accaactcgg ggcatcgatc gcgctggagc
    24241 ggcttgatat tggggactac gccatagggg ataggattct cgttgagcga aagacggtgc
    24301 aggactttgt gacaaccctc gtcgaacgcg acctcttcgg acagattcag gcgatagccg
    24361 atgccgtacc gcgccctgtc ctgatcatcg aggggtctga cgatctctac gcggtgcgaa
    24421 acatccaccc gaacgcgatc agggggacgc ttgccgccat aatggttgat atgggggtgg
    24481 cgctgatccg gacgcgtgat ccggatgaca ccgcagagac gctttatgtc cttgcacagc
    24541 gcgagggggg cgaacggcgg gaacggaagg cgcacccgaa gaagtcctac cgatcgagtc
    24601 gcgaagaaca ggagtatgtt cttgcagcgt tccctaacgt cggtttaaag agtgcaaggc
    24661 tcctccttga gcatttcggg tcgcttaagg cggtcatcga ggctgaaccg ggggaactga
    24721 tggcggtgga cgggatcggg gagaagaccg ccagcgcgat atgggatctc gcccacagac
    24781 cttatctctg acccagtctt gcgagcgccc tcgcgccccg ctcgaccgcc ttcataaggg
    24841 tcaggcagtc ttcagccctc cgctcactcc tgacacgttc gcagaggtgg acgatcgtcc
    24901 gctcgatctc ggcggcaacc gggcgctgcg gctccatggc ggctgcagcg gtttgcggga
    24961 tctgaacggc gggggcagct ggtgcggagg gctcggagcc cggatgaggc tcctcagact
    25021 caaggagagg gcagatgacg cactgcgtct ctcccttgta ctcaaaaagg gggtagccgc
    25081 agaccttgca ggattttgcg agcatcttcc ctcctttgag gagatattcg gccatgactt
    25141 catcggcccg ctcaaccgtc attttgctta actcctttaa tctatatatg cctggatggc
    25201 attaataata gaggggttgt tctcatggca agtccagacg aaacaataca gatctgcatc
    25261 cagatgctgc agaatatcag cgaagattcc accatcccac gaaatatcag gcgtgctgca
    25321 gactcgacaa aatctgttct ccttgacgag tcgcagagta taggtcttcg tgccgcaacc
    25381 gcgatctcaa tgatcgacga ggtcagcaac gacccgaaca tgcccgtcca tgcacggaca
    25441 aggatctggg aactggtatc tcaactcgaa acggtacctc ttgactgaat cgtccacaat
    25501 tgactgattc cggcagaatc ataacagaaa ggggattccc cggttttgcc ggctgaaacc
    25561 cgctatctgc cctcccttag cggtcttcac ccatgatggt gatgtgaacg gtgcgctggc
    25621 gctgcggccg cacgtccagc tccaggagga cgatctgctg ccatgttccg cacgcaagtt
    25681 tgcctccgat gatcgggacg gtgagcgatg ggccgacgat cgccgctctt acgtgcgatc
    25741 gaccgttgcc atcgccccag gcagcatcat gggcgtaatg gatgccctct ggtgcgatga
    25801 ccgagagcgc acggcagagg tcggagagaa cgccgggttc atactcgatg gtggtgacgg
    25861 cagcggtcga acccgagaca aagatgtttg caaggccttc acgcacgccg ctctcggcaa
    25921 ctgtctcagc gaccggcggc gtgaggttga cgatctcccc ctcgccgtgt gtatttacct
    25981 ggattgtccg gcggtgcatc atcactgaca cctctttgac atgctccgtt gtatcccgca
    26041 acttgataaa tgcggcggga gcggccctcc tctgcccggg gggttgggac ggcggggtga
    26101 gcccgcaaaa ccagtttgct catggtttgg tgccctcgaa tcaccaccgt ggtgagacgc
    26161 cttttgcggc tgaacacccc gccccctcca gatggggcgg gcaagtgtct ggtgacaacc
    26221 ccacgaaagc cgtgggcggg gacggagaga ggtgctcggc gattgcccgc tcacgcgcac
    26281 accccaaaaa gccgccccgg cctgaacgtc ttggtttgat ggccgccggg ggtctcaagg
    26341 ctatcgcggc cacaaaccgt atatagaccg tgcgaacatc acctctccgg agatggtgga
    26401 tgtgccctac cggtggaaca gcagaagaga tgtggacgag gctgttgtcg tcgtgatgaa
    26461 tacaatggat tcagaacatg agatcggggg gtggttgatg gagacccttc agggtgcgat
    26521 caacgactcc gatccggaac tcgtgaagca cttcttcagc gaactcgaac agcaccgacc
    26581 ggaggcgctc cggtactttg caaagcccga gttttgagca tgaccggggg agaacgctct
    26641 ccctttgcca gcagcggaac tgtagaacag tcgccatcga caaaaggtgt ggatccggtg
    26701 agaccggtcc gctgcacaaa aaaaacggat ctttcatatc ggccagcggc gcatagggta
    26761 tcatgcccgc aaaaagcgag tttggcagag gatttatcgt caacctgatg ctgctttcgc
    26821 ggcactttgg cctcccgccg gaaagagcat tctatggcgc tgcagaccac ctgaatgatt
    26881 tcatcctccc ggaacgtttc agagggaccg agatcgagga acttgtcgag cggctccgca
    26941 aggcggtgat ctggcaccag cccggtatca tggataaaga ggatgcagcg gacataaaac
    27001 acctcctcaa caggcttgcg atcgcgatcg accgcgaact ggggatcgcc gagcctgacg
    27061 tggggaagta tgactgatga gcgcaggtgc acccgctgat cgaaggcaga gaggcgtcca
    27121 gacctcatgc agtgatgcga tcttcttgtc actggcgggc aggaggatag acgcaaaacc
    27181 cgagaccatc aaaccggttt gctcatggtt cgatcccccc gcatcaccac cccggtgaga
    27241 cgccttttgc ggctggccag ccgcagcccc ctccagaggg gggcagtcta tggcgatagc
    27301 cccgtataga gtgccatggg agcaggaact ggatccgcat gagcgtggtg gtgtaaggcg
    27361 atgccgccac cctggaacta taatggagag gtttatcaaa ccgcggtgga tattgctttg
    27421 atggcctcaa aacaggcatt tcccctgagc cacaggtata agtgtccata aacccaatat
    27481 atagtgtgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcatttcccc
    27601 tgagccacag gtataagtgt ccataaaccc aatatatagt gtgaattttg ctgcgatagg
    27661 gacgccgctc gtttcagcgg aggtcacgga tgactgagac ctacgatgcc tcccacatca
    27721 cggtactgga aggcctgaga cctgtacgcg agcgccccgc catgtatatc ggcagcacgg
    27781 atacccgtgg actgcaccat cttgtctacg aggtcgtgga caacgccata gacgaggctc
    27841 ttgccgggtt ctgcgacctg gtcctggtga ccatcaaccg tgacggttcg gtcactgttg
    27901 acgacaatgg tcgcggcatc ccggtcgacc tcatgccacg gtatggcagg agcgcactcg
    27961 aaatagtact caccgtgctt catgccggcg ggaagttcga caaaaatacc taccaggtct
    28021 ccggcggact gcacggcgtc ggtgtatcgg tggtcaacgc ccttgcgtcc tggcttgatg
    28081 caacagtcta ccgtgacggc aacgtataca cgatgcagtt ccggcggggc gaggtctcaa
    28141 gaccgctcga gagccgcccg gagaccgaag acgagatgag ggcacgctac gaggcacgtt
    28201 acggcacccc ggctccagac gagatagact acgggcggct tcggggaacc cggataacgt
    28261 tccagcccga ccgctcgatc ttcgagaccg tcgagtttga ttacgacgtg ctggatcacc
    28321 gtctccgcga gctcgcttac ttaaacagcg ggcttaagat cgtcctccgg gacgagcgca
    28381 ccggtgactc ggccacatac tgcttcgaag acggtatccg ccagttcgtt gcccatcttg
    28441 gcgaggggaa ggagaaactc cacgacgaga tcatctactt ccagaagagt gatcccgaga
    28501 gcctggttga ggttgaggtt gctctccagt acaacaactc atacacggag accgtcttta
    28561 cctacgtcaa cagcgtcaac acccgggagg ggggaacgca cctcgagggg ttccgaagcg
    28621 ccataacgag ggcgatcaac aacgccgcac gccgaaacaa cctgatcaag aacagcgata
    28681 cactcttccg cggtgaggat gtcagagagg ggcttgtcgc cgtaataagc acccgtgtgg
    28741 caaacccaca gtttgagggg cagaccaaga tgcgccttgg aaacagcaac gtccggggca
    28801 tcgtggactc gctggtctac tcctcgctca ccgactactt tgaggaacac ccaaaaaccc
    28861 tccaggcgat cgtggagaag gcgatggcgg ctgccagggc gcgggaagcc gccaggagtg
    28921 cacgtgaact cgcccgccgc aagagttcgc tggagtcgac cgggctcccg gggaaacttg
    28981 ccgactgcca ggagcgtgac ccggcaaaaa gtgaacttta catcgtggag ggtgactctg
    29041 ccggcggttc agccaaacag ggccgcgacc gtaagttcca ggcgatcctt ccactgcggg
    29101 gaaagatact gaacgtcgag aagtcctctg agcacaggat cctgaagaac gccgagatcc
    29161 aggcactggt ctccgctatc ggcacaggca tcggcgacac cttcgatgcc gaacgcgccc
    29221 gctacaaccg gatcatcctg atgacggatg ccgatgtcga cggggcacac atccggacgc
    29281 tcctcctgac gttcttctac cgctacatga cagggctcat cgagaaaggg ttcatctata
    29341 tcgcccagcc accgctctac cgggtcgcca ggggcaaaca ggaaagatac gcctacggtg
    29401 atgaggaact ccgggccatc ctggcggatt ggggtgagaa gggcatctca attcagcgct
    29461 acaagggcct tggcgagatg aacgcagagc agctctggag caccacgatg gatccggcaa
    29521 acaggatcct caagcaggtg cggatcgagg acgccgcata cgcaaacact atcttcgaaa
    29581 aactgatggg agaggatgta gacgcccggc gggacttcat acgccggcat gcaaaagagg
    29641 tgagcaacct tgacatctga gcagcaggtt gtccctgtca acatcgagga ggagatgaag
    29701 tcatgctaca tcgactacgc gatgagcgtg atcatcggcc gcgccatccc cgatgttagg
    29761 gacggcctga aaccggtaca ccggcgcacc ctctacgcca tgtgggagat gggcaacaca
    29821 agcgacaaac catacaggaa gagcgcccgt gttgtcggag atgtcatggg taagtaccac
    29881 ccccacggtg atgcggccat atacgacacc ctggtgaaga tggcccagcc cttcacctac
    29941 cgctacccgc tggtggacgg tcagggaaac ttcggttcga tcgatgggga tgccgccgcg
    30001 gccatgcgat acaccgaggc ccgcctcacg aaactctcgg aggagctcct ggccgatatc
    30061 gacaagaaga cggttgattt caccccgaac ttcgacggat cgcttgaaga gccggaggtt
    30121 ctccctgcac ggataccaaa cctcctcatc aacggttcaa gcgggattgc cgtcgggatg
    30181 gcgacaaaca tgcctccgca caacctccgc gaggtatgtg aggctatctg ccgtgttatc
    30241 gataacccgg gtctctcgac ggatgaactg atgaggatca tgccgggacc ggacttcccg
    30301 acaggcggga tcatcatggg gacggagggt atcagggacg catacctgac cggacgcggg
    30361 aagtgcgtga tccggggtgt cgcggagatc gaggaggagg gcaggacgcc gcggatcgtc
    30421 atcaccgaga tcccctacca ggtgaacaag gcgcgcctga tcgagcatat cgcctccctc
    30481 gtccgcgaca gaaggatcga agggatcagc gatatccgtg atgagtccga ccgggagggg
    30541 atgcggatcg tgatcgacct cagacgaggg gtggcccccc aggtgatcct taattacctc
    30601 tacaaacaca cggcgcttga gtcatcgttc gggatcatca acctggcgat cgtcgatcgt
    30661 cagccccgca ccttaaacct caaggagatc ataggggagt tcatccagca ccgcgtggag
    30721 gtcgtccggc ggaggacaga gtttgatctc gcgaaggcac aggagaggat gcacatcctc
    30781 cgcggtctcc tcgtcgccct cgacaacatc gatgctgtgg ttgcgacaat cagggcttcc
    30841 aggacgacag aggaggcgca gaaagcactt gttgcacagt ttgcactgga cgaggtgcag
    30901 gcggacgcca tcttaaagat gcaactccgg cggcttgcgg cgcttgagca gcagaagatc
    30961 ctggacgaac gcgacgttct tgcggccgag atcgagcggc taaccacgat cctctcgaca
    31021 gaggcaaaca tcctcgctga gatccggaaa gaacttcgtg atattgccgc ccgctatggt
    31081 gatgatcgta gaacccggat cgtagaggcg gccgagacgc ttgagaagga ggacctgatc
    31141 gaggacaaac cgatgctggt ctcgctcacc gcttcaaact acatcaagag gatcgacctg
    31201 gatgcctacc ggaaccagag gcgcggcggc cgcggcgtca taggcatggc cacaaaagag
    31261 gatgacggtg tagagagggt ctttgcagcc agcatgcacg accacctcct ctgtttcact
    31321 gaccgaggaa gggtctactg gctaaaggtc tacgaccttc cggaggggca gaggacgggc
    31381 agaggcaaag cccttgtcaa tctcctgccc ctcaccggag atgagcgtgt taccgcggtg
    31441 atcccggtca gggagtttca acctgaccgc tacctcttct ttgcaacgag aggcggcacg
    31501 gtcgcgaagg tggcgctcga tgagttctcg cgtccgcgcc agaccgggat caacgccatc
    31561 aaactccggg acggcgatgc gctggtggac gtgaatatga ccgacggcaa ccaggaactg
    31621 atcctgacaa ccaggttagg ccagagcctc cggttccacg aagactccgt ccgcccggtt
    31681 caccggaacg ccatgggtgt ccgggggatc agactgcgca acggtgatgc gctgcagtcg
    31741 gtgacggtgg tggaggggga tcatcttctc accatcaccg agcagggttt cggtaaacga
    31801 accgagtttg atgagttccg cggccacggc aggggcacgc tcggcgtgcg aaacatcgtt
    31861 gtcgatgcga ggggtggcgg tgtcgtcggg tcgatggcgg tctatgagaa cgacgagatc
    31921 atcgttatga gtgcctccgg gatcgtgatc aggacgaagg tctctgagat ctcgatacag
    31981 aaacggggca cgcggggtgt ccggataatg aggctcgatg atggtgatcg cgttgtcggg
    32041 ttcacgatcc tgaattcaga agacagggac gaggagtagg cgccagggat cacgcggccc
    32101 agtatgaaaa cccgggtatg ggctgccctc tctccccgga atatccagac cgccactgaa
    32161 tagccatcag ttcatctccc cggccactgc ttttgacggt ggaacttctc cccccccgcc
    32221 agccaggccc ccggattggc ggttcccagg cagaatttgg gtgcagcctc cacgcacttc
    32281 cgccgctgga agagatggac gccaccagcg gcactctatg ggtgcaggta tatcagcacc
    32341 gttgatagca cgataaaacc gagtgttgcg gctgcaaacg ctctctcacg ggtatttagt
    32401 ggcttcttgc gtatatagag gccagccttt cccccgtagc cccggcagag catgcttgta
    32461 taggtgcgct ccccctgctc gtatgaacgt ataaacatcg ttcccacggt gtagccgacc
    32521 tggtgcagcc tatagcgata cgggaggctg cgatcgaggg gatcgaaaca cctggtcgac
    32581 atcgccgtcc gtatcctccc gaacatctcg gcaaagacaa agatgtagcg gaccatcatc
    32641 ccgagtgtga gggtgaactc tcgcggcaga ccgagcctcc ctgccgcctc gatcatatcc
    32701 tgcatcctgg ttgtcgagga gagcaggatg atgaacgata tgctgacgat gaacttcaca
    32761 aacaggatcg acgcaaactc cagcgactcc gcgtagatct cgagcccgaa tggcagcgtt
    32821 gcaaccgtat gaaacgtatc gtaatagggg ttcttcacaa aggcctggaa ccccaccagg
    32881 aatatcccga acggcatgag gagagcaagc ctccggaggt agaccagcgg agagaggcgt
    32941 gatgccgccc agagaaggag aaagagagcc aggaaagcgg cacccagggt gtagaccttc
    33001 gtagagtacg gcagcgccac gatggccacg atggcagcaa gggccaacag gagttttatc
    33061 cttgcatcca gtcggtggat gacgcttttt ccgtaagcgg acctctcgat ggagtacaga
    33121 tcttcgatca tctctctcgc atgtaggcat caaggaacgc cttctcggca tccttgtagg
    33181 tgtaggccat ctcgatctcc acaccgttct cctggagcga ccggatcagc ctcggtatgg
    33241 gcggcacatc gagtttcgcc cgggtgagaa gttccggtct ggcaaagacc tcctccaccg
    33301 tcccggaacc catgatcttt ccccgatcca tcacatagat gaagtccgcc atctccgcca
    33361 ccaggtcaag atggtgggtc gagaagatca cggtcatacc gtaatcctcg ggcagccggt
    33421 tgatgaacgc cacaaggtca tcgacgccct gggggtcaag cccagcggtc ggttcgtcga
    33481 gcaccagcac ctgtggctcc atcgccagga tgccggctat ggcgacacgt ttcttctcgc
    33541 cgccggagag gtggtgcggc gcgcgctcgc ggaggtcctc gatcccgagc agacgcagcg
    33601 cctcctcgac acggtgggcg accgtctcct catcgagacc aaggttgatc gggccgaagg
    33661 ctatgtcctg ctccacggtg ggggatatga tctggtcgtc cgggttctgg aagacgatcc
    33721 cgacaaactt gcgcacctcg cggagattat cctttgtgat gggctcgccc ctgaccagca
    33781 ccccgccgga ggttggtttg aggatgccgt tgaagtgctt gaagagcgtg ctcttcccgg
    33841 caccgtttgg cccgatgaca gcgattcgcg cctttcgctc tgcaataaag ttcacgccct
    33901 ccagagcgcg gacgctgccg cggtagacgt gggtgagttc acgggtctcg atcaggtgca
    33961 taacaacaaa aggttgggag tttttgaggt atctaattgt ctggccgggc agagaccttc
    34021 gcaaccgcaa acaccagtcc cagcatgagc agcgtcccga tcacgatcgc aagcacctcc
    34081 cccgccttat ccatcccggg tatgctgtaa tccggcatca ggggttcatg cgagaagtca
    34141 tttcctgtca ccgccaccac ctcttcctct gcagcggcgg cagcctcttc gtcaaggtcg
    34201 ctgcccatga agggcttcgc accgtacata ctgaagaacg cgctctcaag cccatcgggg
    34261 ttgctcgacg cgaggaaggg cgcagcgatt gcgatcacca gagcaactgc gatgccgatt
    34321 atgacaaaac gttttgcctc cattacgcgc ccacccccag agggtgttca aggatgtccg
    34381 gtcgtgccga ggctatcagg taaagtgcga ccgcagtgat acctccttcg ataagaccga
    34441 tgatggcgtg ataggcgccc atcatggtaa gcccgacagt gagggggaac gtaccggcga
    34501 ttgcaagttc aactgcacag aggattgcag agataaagag cgacgcccag cctgcgatga
    34561 atgctgcagc gtaggcattc gagatcagcc tcctgaccgc ggtgtaaccg tagtagccga
    34621 tgaacccgcc gatcacaccc atgttgatga tgttcgcacc catcacggtg ataccgccgt
    34681 cgccgaagat gaccccctga accaggagta ccagtgtcag gatgaagaca cccgcatacg
    34741 gcgaaccgag gacaattgcc gcaagtgccg caccgaccat atgcccgctc gtcccgccgg
    34801 ggatggggac gttcaatgcc tgaatggcaa agatgccggc ggcgagcacg gcaaccaggg
    34861 gtaccttctc ctctcccatc gaccgccgcg cccagcggag agcgagggca acaaagatca
    34921 gggctattac ccagtagacc agggcctgcc cgataggtat gaaagcatca gggatatgca
    34981 taacacatct gatgcgattg tagcactttt tagtataaat gtgttactaa acaatatggt
    35041 tacctcagcc ggtaataatc cggcgtactt ccgatgcgaa aagtataccc atagccctga
    35101 ccactgccgg ttttaaggca agtttgcgaa gcaggctgac cggcctgtcc atgtcgccgt
    35161 gctccacgat cgtccttatg acatccgggc tgtcgagagc acggcatagg cggtcgatct
    35221 cctcgggcgt catacgctgg cgcagcctga gcgctttcat cccgatatcg agttccctcc
    35281 cgaaatcttc cctccatcgg cgctcatact cacggagatg ctcgccatca aacgtccccg
    35341 ccgagcagca tgccgccgca acggcggccg cgtgttttgc ggaacggacg ccggtgtaga
    35401 ccccgccgcc ggaggtgggt tttgcaaacc cggccgcgtc gccgacgatg aacgtccggt
    35461 gaccgcaggt ctgcggcatg accccgaggg ggatgacacc tgtcacaagg tggagcatgc
    35521 tccccccgta cctgacgatg aaacggtcaa aatattcttt tgcatgctcc ctggcgcaga
    35581 ggccaactct ggcccgggtc ggcgagaccg gtatcgccca cccaaagaag tcaggcgagg
    35641 cattggggtg caactcaacg caacgcgggt cgatggagca ggggacttcg gcctggatcc
    35701 cggcaaggta gacctgcggc cgccggaacc cgagcatacg ggagatggag ctccgcgggc
    35761 cgtcggcggc gatcaggaga cgaaacggga actcctcgcg accgcgaacg ccgcgggtca
    35821 ggagagcggt gccccggata ccggccgcgc tggtcttcaa ccggaactcc gccccggcgt
    35881 ccgccgcttt ttcggccatc tcgcggtcga ggagaccgcg gtcaacgaca aatgcctttg
    35941 tgacacccgc atcgaaggaa atctccccgc cgagcccaga gaacattcgg gcgccgctca
    36001 cctcgttcaa gatggagcgt ctcgatacac cgcactccgc aaacgcggag gtcgagagga
    36061 gcccggcgca ctggacggga tggccgattg tcccgtgctc ctcgatgcag agcgtcgaga
    36121 gcccttcctc agcgcaggcc cgtgctgcag cgctcccggc aggccctgca cccacaacga
    36181 taacatccca cgtcagttta tcacgcctca aaagggtatc tgccccatgg ccattcttca
    36241 gagaggtagc atatcacaat aataaactct ctctgcagag ttttcaaatt tattggttct
    36301 gaaagtattc cagggccttc agtcgcccga gaccggaagc gtcaattcct cctatcaggc
    36361 aaattgcagc agggagttga ggcgacagaa acgtatagcc tctccagagc acgagccgta
    36421 agcggcatcc tcgcgccttc gtcacgataa cagaaaggat gcaccaaaag aggtgactac
    36481 tggagatgtt ctgccaccga ccgcccggct tccaggccta tgagcgcaag gaagattatg
    36541 gtcagcacga gcatcaagga gaggaagact gcgatcgttt cgttcagggg ggcattgagc
    36601 accccggcaa caggggttag aatagtcgga aacgcggcaa ctgcagcgga ggcagccacc
    36661 catatagcca caaaaatgag gtagattgag atcctcttca taggcctact cttcgcggcc
    36721 ctcattccac tccatctctt cccggtgcga ctcgatcttt cgtgtgacat cagcagccca
    36781 ccgctcgatc tccacggaga agagtctgcc tgtccggaag aggacaagga ttgcccagat
    36841 gaccaggacg acaagtacta tgcccgcaag aaccggtatg atccctgcga tgaacggcag
    36901 taagaacaga aatagaatga cggcaaggag cacgtatgcg ataccccgga acgcccgctg
    36961 gtaacgctcc agtttctcat caggggtctc ttttctggaa tacgccgctg cagcataccc
    37021 tgcgagagca ttggtgatgt caacgacctc cttgaagatg ccaatgaaga tgagcaagag
    37081 tgcgatgaga attatcgctg agatcagact cccgagtcct atcaggctaa agatgagcgg
    37141 atcaccgagc atccttgcaa gcggctgaaa gatcagcaca ccaaagagcc atatgagaaa
    37201 tgcgacggca atgccgacga cgatcttcgg aacactgtcg tatatctctc gcctggatgc
    37261 ggcaagtaac tcttcacggt ctaccacaaa tttcacctct ttcgagagtt gcttttgact
    37321 cccaatgtat ttattctttg ccctaacagg ggcctacaaa aggagcgatg gcgtaaaaaa
    37381 ccgtttacga taaaattttg catatatcct ccggaaaagc gtgtcggtcg ggctgaaaac
    37441 agatctggga acgtcccgcc gagatgcggg cggtattgtc acccccgcac acagatgcgt
    37501 atttgacgga tgatacccaa gatattacgg aatgcctgag gaacgggagt tcaagtggaa
    37561 acagtgcctg attgtgcgta ccgacatcaa gatgacctgc ggtaagaagt gcgcccagat
    37621 cgcccacgcc gccgtgagcg cctttgagaa ggccgataag ataacgaaga aagcctggct
    37681 ggatgagggg cagaagaagg tggtgctgaa ggtgccatca gagcgcgacc tctttgaact
    37741 caagacgatt gccgagcgtg cgggcatccc ggcatcgatc atccaggatg ccgggatgac
    37801 cgagatcccg ccgggtacgg tgacggcgct cggtcttgga cccgcccgct ccgaagacct
    37861 ggacaggatc acaggcgacc tcccgctgct atgatgccat caacccatcc ccttgaacgc
    37921 gatctcggga tgcgctacta cgcctcggat actcccggga tcggcggccg actccgttca
    37981 acccccgcag acttcatcgt tgaggaactc cctcttccaa tccccgacca ggaaggaccc
    38041 tacctcatat gcagactcac caagattaac tgggaactcc agcgcgcggt caaggagatt
    38101 gcaaaacgtc ttggtataag ccaccgccgg atctcctggg cgggaacgaa ggacaagaac
    38161 gcggtgacaa cccagttcat atccatctac gacgtctctt tagacgcgat cgagcgggtg
    38221 aatatcccgg atatatcact tgaggtcgtg gggcggtcgc aacacccgct ccgcctcggt
    38281 ggtcttgcag gtaaccggtt tgaggtcatg attcgggagt gtctaaaaga ggacctcccg
    38341 gcacgggtgc aggcggtcac ggaggcggtt tctgccggga taccaaacta ctacgggttg
    38401 cagcggttcg gcgtaatccg accggtcacc cacgttgtcg gtgagaggat actcaaagga
    38461 gattatgagg gtgctgttgc cgcatacatc ggccgggcat acccgatgga accagaggag
    38521 gtgcagcagg ccaggacgcg gtttctcgag acgggggacg cacgagcggc gcttgccgac
    38581 ctccccgtga ggatgacata cgagcgggcg atggcaaacc acctggcagc aaaccccgga
    38641 gactacgccg gcgcgctccg agtcctcccg ccgaaactcc tctcactctt tgtgagcgct
    38701 tttcagtcct gcctctttaa ccatgcactc tccgcccgca tagatgccgg tctctcactc
    38761 acagaacctg aggtcggcga ccgtctactc tttgaaaatg gccgtgagga tgttgtcaca
    38821 gcacgaaacc agcaggcggc ccgaatccag atccggcgcg gacgctgccg gatagccctc
    38881 ttcatcccgg gtgctgaacc ggtgagagtg tacgggccga tggatgatat cctgcacgac
    38941 ctgatgcagg ggcgcggtat cgacgccgcg gactttgagc gtgcctcaag gtttgtggag
    39001 acggcgttct cgggggttct acgcgcgata agcctctcga ccgatgttga ggcggaggtt
    39061 gccggcacgg atctcaggct ccggttcagc ctcccacccg gtcactacgc aaccacggta
    39121 tgccgggagt acatgaaagc cgagccgtac ctgatgatat aggggcagag cagagcgcga
    39181 agacgcaatg cgcaggcggg gaggggaggg actttcacgc ttcactctcc ggaatcggga
    39241 cggtagagga cttcccactc ggtgaccagg ttgctaccgc acatgtagta acgtttaagg
    39301 gagagccgga acatctcctc ctcggtctcg atgtgcatgc cgccgacaag cgagggggtg
    39361 tcggcaccgc caacgataaa cgggaggtga atcaggcgta tctcgtcgac aagatgatgg
    39421 tgcagcatcg accagttcag ggttggcccg ccctcgatca tcatccggtc aacaccgtag
    39481 tcttgcttca ggatctgcat cagtcgcggg aggtcgacct gactcgtgcc ggcgacgacg
    39541 acatcgacac cggcatcccg gagccgggcg atgttctcag gcggcgcggc ctcactgacg
    39601 acgataagtg ttcgagcatc cggacccagg atgtttgcac ccggcgggat ctctccgcgg
    39661 ctggtcggga tgacgcggag cgggttcttc ccctcgacca gcctgaccgt caggaaggag
    39721 ttgtctatcc tgatggtgtt tgccccgacc atgattgcat cgcattctgc ccgtgtccgg
    39781 tggaggagaa cctcagtctc gggggccatg tacttcatga ggatcttgct tgatgccccg
    39841 cgtttcaggg tgagcttccc gtcgacggtg atctccgaca tcatgagcac gtgcggcctg
    39901 tcgtcttgtg gcattggact cgttcccgga tactatctag cgggagcgtt tatgaatctg
    39961 gtgattcacg cccggaaagg gtattctggc tgaagcgttg cgcctctatc cggcagagca
    40021 aagattaaac actttcattg ctgattagat atgctatgaa tataacctcc ctgcggccga
    40081 aatttgttaa atgcacggag ccaattgcgt tgttttgcat ccggctgggc gttacaccaa
    40141 accagatctc ggtgctctcc ttgctcttcg gttttgggtg cgccatcgct tttgccggcc
    40201 gccacttcgc cgcggggagc ctcttcctcc ttatctcagc cgtcctcgac cttgtggacg
    40261 gcagcgtcgc gcggcggaac aataccggaa gcaggttcgg cgctgtcttt gactggatcg
    40321 ccgataagta cgttgacgca gcagtcatac tgggtgtggg tttctccggc atcccgattg
    40381 tcagccacat attcgacgtt ccggcggttg cggatttcgg cgtcgtgggg ctggcgcttg
    40441 cagggtcgct gctgaacaca ttcatcaaac cggtcactta tgcagagatc ggctactctg
    40501 agcggatcgc cggaaagatc gaggatccgc tggagggggt cggcttcttc ggcaggccag
    40561 agacaatcct tgttttgcta atcggcggca tgaccggttt tctctgggtg gcggtgatcc
    40621 tgatcgcggt ctgcacaaac ctctccgcgc tccagcggat cgtctacctc taccggcggt
    40681 attcctgagc ggctctccct gttttccggg cgttaatatc cggcaaatgg agcatcatgc
    40741 cgcagcgctg acgttgggga ttgaaatgag accggaggag tgggggtcaa gaagcaccag
    40801 gcaggaaccc cgccgggttt tttttcacgt cccggtcttt tccggtcccc gccggaggag
    40861 cctttccagt tcgccgcgat aaagcagata gaggtcgttg gcgagggtgg ccagttgagg
    40921 tattgcggtg aagagggcgt aggccagagc aaccagcacg acaagcgccc cgagttctgc
    40981 catgacgttg tgcgcccaga agaaactctc gtagccgaac tcgccgttgc tggcgatctc
    41041 gggaaggtat gggaaccact gctcggtgta ggccgtgatc acaaagacgt tccggagtat
    41101 gttgaggatg tagatcgtgg ggaagacgat gaagaaaccc gctatcttct gtttcagcgt
    41161 tgactttacg ccccaggcga cgccgagcat gatcgcgatg ctctggatcc cggtgcaggc
    41221 gaggactatc tcggtctgga aaccgttgcg ctccagcatg ttccaggcct gaaaattaac
    41281 agggtagccg atcgccgaaa gcactgccga ggtctgtccg gcgaccgcgc tgatcagcca
    41341 gttcccgagc gccgggatgt agccgaaagg agcgtagacc aggaacgcta cggcggcgat
    41401 catcgagacc tggatcaccc gggggtcgtc gcggaggagg tattttatgg ttatataaag
    41461 gagaggcagc gagaggattg ccagcgttgg gtatacaaag ttattgagtg agatatagcg
    41521 agggaactcg gcgaagaggt agccaactat gcttacccag ccgccgatgg caaaatagtt
    41581 tcgatacctc caggggatga ggaaggcaag aaagcagatg caggagatca gcaccaggta
    41641 atcactcatc cattaacttt ctatttatcc ggcaataaaa ccacttgtgc gcccgatctc
    41701 cccggccgcg ggtttgggcg agggagcggc ccgtgcagca tgaccaaggt taattgtggt
    41761 cgccgtccaa taatcatacg atgatgttct gtccgcagtg caaaggtctg atgatatcct
    41821 caggtggtca gctgaagtgc aagaagtgcg gctatatccg tgatatcaac gcgtctgacc
    41881 agttgaagaa gacagacaga cgggtggaga aggagatcac catcgtcgac gcggagaagg
    41941 agatcgcaac tctcccgacc acgaccatca aatgccccga ttgtgactgc acgacggcat
    42001 actggtggct gaggcaactg cgtgcggcag atgagagcga ggtgcggttc ttccgctgca
    42061 cggcctgcgg gaagacgtgg cgcgagtacg attgatgttc tcttttaccg gccacgatcc
    42121 agcgatggct gattctctgg tctggtaaga ggtgagtcgc tgcttttgtt ttccatgccg
    42181 ggagaagcca acaggaaatt atatatctcc ccgggagtca tttccaggca tatggggcca
    42241 gcatatcgtc tggaaatgct gtatgttatt attcatcgat accgaaacga ccgggacgtt
    42301 caggttcaat catccaccac cccgtcttgt tgaggttgcc tggctggtct gtgaggagag
    42361 cggggaggtt gtggagaggg gcgacaggat cgttcgcccg gatgatttta ccatcccggc
    42421 aggcgcctcc aggatccacg ggataaccac aggatacgcc cggcgggccg gtgtgtctct
    42481 cctggaggtt attgaggctc tctccattgc cgcaacccgg agttctacgg ttgtcgcgca
    42541 taatctcagg tttgaccgtg cgatcatcgc aggagagtgc aggcgcagcg gtgtttcaga
    42601 tcctctctcc acgctgccag ggatctgcac aatggagtcg acgccgtcag tctgcaggat
    42661 tcggcggggt ggcggcagtg gctataagtg gcctgctctc tccgaacttt acgcgagcct
    42721 atttggagtc ccttacgagg gggcggttca ccgtgcttcc ggcgatgtag aggtgtgcgc
    42781 aaaatgcttt tttgccctta aggaacgagg cttctggaga taacggttcg gtttatctcc
    42841 acaggtgtgg tggaacacag gtattggtcc agtgtgagtg tataccagcg tcggttcatc
    42901 ccccacccac ggggaaacac cttctcagtg gagggaagtt gccttccaga gagggtcatc
    42961 gatccccggg acaccctccg gccgcaaggc tgcagcaact cttgccatag cagcgtatca
    43021 ccgttctggc aacccccctc tgcgccccgg ccgccgcgct gaaacaacct gcgcactaac
    43081 ccggcccgca gcctggtaac gggccccctc ctcgcccgcc agaatttact ctaacgtcct
    43141 ccgatcccaa aaaaacgtcc aggaattagg caatcccggc tccaccggcc ccgccggatt
    43201 ccgggtgcgc cccggtaacc cttttccctg cagcaaatca acgcttaacc agaatgttga
    43261 tataatattc attattaata atgatcaata cggggtaaac gaatggctga gaaatttgat
    43321 gtcaaacttg aggaagcgag atactacatg cccgatgcca gttacaagga gcagtcctgg
    43381 ataggcgact acaaccgcgc ctaccaggag ttcctggctg accccgatgg attctgggac
    43441 aggatcgccc atgaactcga gtggttccag ccctgggacc gggtgaaaga atgggactac
    43501 ccttacgcga agtggttcac caacgccaag ctcaacatca cctacaactg ccttgaccgc
    43561 cacgtcaaca accagcgtcg caacaaggtc gcgatcatgt ggcgtggcga gaccgagggt
    43621 gaggaagagg tctacaccta ccgccagctc ctgcaggccg tcagccagtt cgccaacggc
    43681 ttaaagagcc tcggcgtcgg caagggcgac cgtgtctgca tctacatgcc ggtcgtgcca
    43741 gagcagatca tcgccatgct cgcctgcgcc cgcatcgggg caatccacac ggttgtcttc
    43801 ggtggattcg gtgtcagcgc actcaaacag cgtataatcg gcaccggtgc aaaggttgtc
    43861 gtcaccgccg acttcacata ccggcgcggc aaggcaatcc cgctcaagaa catcgtcgaa
    43921 gaggcggtcg tcaacgtccc gagcgtcgag cggatcgtca tcctcaggcg tgacgagaaa
    43981 aagcccgtag agctccaccc cgagatagag attgacttcc acgaactgat ggaagaagca
    44041 gacagggaat gccctcccga agttatggat gccgaggacc cactgtttat actgtatacc
    44101 agcggcacca caggttcccc gaaaggcatc gtgcacacat gcggcggcta catggtcggg
    44161 acgtactaca caacgaaata cgtctttgat atcaaggata acgacgtcta ttggtgcacc
    44221 gctgacccgg gctggatcac cggccacagt tatggcgtct acggtccgct cttaaacggc
    44281 gccactttcc tcatcgcaga ggtcaccccc gactacccga caaacggcac ctactgggat
    44341 atcatccagg actacggcgt caccatcttc tacaccgccc cgaccgccat caggatgttc
    44401 atgcgggtgg gtgaagagtg gcccaatcgc tacaaccttt catcgctccg cgtcctgggg
    44461 tcagtcggcg aacccctcaa ccccgaggcg tttgagtggt tctatcacgt catcggcaaa
    44521 acccgctgcc cggttgtgga cacctggtgg cagaccgaga ccgggatgca catgatcaca
    44581 accatgatcg gcgaacccat gcgtcctggt tttgccggga aaccgatccc gggcgtcgtc
    44641 gccgacgtag tcgaccacaa tgggaacccg gtcgctccag gcacaggagg gtttctggtc
    44701 atcaaggagc cctggccatc gatgatgcga acggtctgga acgacgacga acgttaccgg
    44761 aaatactggt acaccatccc cgggtgctac accgcagccg acctggcggt gaagaaccac
    44821 gatgactaca tcatgatcct tggcaggtcc gatgacctga tagtcgtcgc cgggcacaac
    44881 atcggaacag cagaggtcga gagcgcactc gtctcccatg aagccgtggc agaggccgcc
    44941 gtcattggaa aacccgacca gctgaagggg aacatcatca aggccttcgt catcctcaag
    45001 aatggtcgcg tgccaggcga gaaactgaaa aaagatctcg tctaccatgt ccggatgacg
    45061 cttggaccta tcgccatacc ctcagagatt gaattcgtcg attcactccc caaaacccgg
    45121 agcggaaaga tcatgaggcg tgtcctgaaa gctcaggaac tgggtatgga ccccggggac
    45181 atatcgaccc tcgaagagta gaaacccaac catttatata cctattttaa atatgctata
    45241 tatatgtctg aacacagacc agaggagtct ctcaagatag agaatatcgt tgcttcagca
    45301 aaagtgaccg actctctcga tctcccctcc cttgcttccc gcctgaaaga tgcagaatac
    45361 aataaaaagc ggttccctgg cgttgttctc aggatgcagg acccgaagat cgcagcgctc
    45421 gtcttcggtt cggggaaggt tgtcctgacc ggcgcaaaga gcatagacag cctctcacgg
    45481 ggactgcaga tcctcacaga ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45601 nnnccggcgc aaagagcata gacagcctct cacggggact gcagatcctc acagaccagc
    45661 tgcgagcgct cggcatcgag atccctggaa acccggagta caagatccag aatatagtca
    45721 cctccgctga ccttggaacg ccgatcaacc tcaacaagat cgccgttggc tttaaccttg
    45781 accggatcga gtatgaaccc gaacagtttc caggcctcgt ctacagactc gacgacccaa
    45841 aggtggtcgc cctcctgttc ggttcaggca agctgatcat caccggcggc aaggtgcccg
    45901 atgatgcacg cagagcggta gagcgtatcg tctcagaact ctcgagtctt ggtctcctct
    45961 aacaccaggt gggtctggca ctcacccaat ctttgcggca gaagatttga accagcagga
    46021 ttctcgcgca aaaagcaatt ttgagacttc cgcaattgct gccggcctca ttgaaacaat
    46081 aatattttta tagtcagatt taaaacacta gatacaaatc aatggtgaat ggatgggacc
    46141 caagacgacg aagagtttta ctccacgtga cgaggaagtt gccgaactct taacggacat
    46201 cggaattaag agaaatgtcg cgagggtgct tgtatttctc acaaacaacg gtgaagcaac
    46261 gtcaagagag atcgagcgag gcaccgacct ccgccagcca gaagtcagca tcgccatgcg
    46321 ctacctcaaa gaatgcaact ggatcgatgt ccgtgaaagc aaatctgaga gcaagggaag
    46381 acctgtaaag atctatactc tcgccaggcc gatatcagag atcatggaca cgatcgagta
    46441 cgaaaagaag aaagaagccc ggcatcagct ggaactcatc cagaggatgc gggattgcat
    46501 atcaacccag tgagaggatg cggcaaccgt tttcctcacc tacaataacc gttatctttt
    46561 caccgtagat agagaagagc ccgcatccca tcacgcccgg gatcgatccg atctccacct
    46621 caaggcgacc cgggtcggtg atcgccccga acccgcagtc gattatgaag ttgccgttat
    46681 cggttatcac gggcccatcc tttctcaccc cttcccgaag cacagcatcg gcgccgagcg
    46741 cttcaagcct ccggaagacc gtcccggcag caaacgggag cacctcaaca ggcacgcctg
    46801 catccagggt ctcaaccatc tttgaggggt cgacgacgat caggacctcc ctggctgcat
    46861 cgcagacaca cttctcccgg aggagcgccg ccccgcgtcc cttgatcaac cggaacagcg
    46921 ggtcgacctg gtcagcaccg tcaatcgcca gatcgagttc ggggtgctca tcgaggtcgg
    46981 tgagggggag gccgtaacgg cgcgcccgca tggccgcctg gtaggatgtc ggcacaccgg
    47041 caattgagag cccctcctcc gtgatccgct caccaagccg ctccatcgca aagaagaccg
    47101 tcgaccccgt gccaagaccg acgaccatcc cgtcacgcac ctcgccagcc gccctgtagc
    47161 ctgcgatcct tttcgccgca accggatcga gtgcgctcat ggcagactca cctccctttc
    47221 tcctgcaatc atctcaccga agatgcgctg gagatcttct gcggcatcaa aagccgtgca
    47281 gtcgagcgtg atcggcgtta tggagacgtt gccctgctgc acagcgtgga cgtcagtccc
    47341 ctcttccgca tcctcgtaga gcgggccgtc gatccagtag taggggcggc cgcgggggtc
    47401 gaaacgctcc tccaccccgg tgtggaagag ttttgcagca agccgggtga tctcgtagcc
    47461 gccgcgcacc tccgccggga tgttcacatt gatgacgtgg gcttttgcag gaaacccagt
    47521 ctcaagaacc ttcccgcaga cctcgcggac cacccgttta gcgccggaga accgtctggt
    47581 gatcccggaa ggttcatcaa acttgtcccc ctgatcctcc acctggagcg agaaggcaat
    47641 gcccggcaca ccctggttcg aggcctcgag cgcggccccg atcgtccccg atgtcatgac
    47701 tgactcaaac gagaggttct cgccgatgtt gatcccgctg accaccaggt cagggttcag
    47761 gcgcagcgca aacagcccga tgatcacggc atcggtaggt ttaccgccga ccgagtaggc
    47821 ggtaacaccg tttatcatca ccctgtttgc acggataggc tcaaatatgg atatagaccg
    47881 ccccacagcg ctctgctgcg tggccggagc gaccacggtg acgtcagcaa gcggcgcgag
    47941 cgcattgtat gccgcccaga gccccgtgga cgtgatccca tcatcgtttg tgagcagaat
    48001 cctgcgtttc atcttgcata taggggtggt cgccccgaac caaaaacctg cccgatcgtc
    48061 agggtcatgt ggtgggcgat caattatata ctggaatgaa ggtctttctc gccgaataca
    48121 ccgtctgcaa cgaccccgac cttgcgccgg agggcgccgc gatgcttgat acactcaccg
    48181 ggagttttga acgctgcggc cacgaggtgt tcaccccgga gaagcctgat ctcgaggccg
    48241 agatccggag actggccccg caatccgatg cgggactggt catagcaccg gatcatctca
    48301 tatttcgcta cacccatctc ctcgagcagt tcacccacaa catcggttgc gggagcatga
    48361 acgcggcggt ctgcgcaaac aaaccgcgga cagcggcgat cctctcccgg aacggtatac
    48421 ccgtccctcc ccctcccgcc gccggggggc gcagggtcgt caaaccgatc atgggctgcg
    48481 gctcccgcgg ggtcaggctg acggagggtg aacccggcga gggcgagttt gccgagggtt
    48541 acgtcgatgg ggaggcgctg agcgtgagcc tggtggggag ccgggtgacc gggaacgtct
    48601 gcgagttcta ctcgggcgcc ccgccgctcc tgctcgcgat caaccgccag gagatcgctg
    48661 tcagcgacga cgggctgttt cactacatgg ggggcgagac ccctgtccac ccggaaaggg
    48721 aggatgagat agtcgaggcc gccatcagga cgatgaacgt tctcggttgc caggggtatg
    48781 ccgggatcga tctcatcgcc ggtgaccgga tctacgtcct ggacgtcaac ccgagaccca
    48841 ctacaagcct tgtcgggatt gcggccgtca tgaaggagga gatcgccgat atcctcctca
    48901 gagcatcgta cggcgacgcc cccggagagg tgcacctctc cggcagggtg cggtttgaca
    48961 gggacggggt gatccacagg ctatgatcgg gatcgatata gggggggcaa acctcaagat
    49021 cgtcgatgaa gacggcgtcc atatccatta ctgccccctc tggcagggtg cgccgctggc
    49081 cgaacttctc agagactgca ggcgtcctgc tgccgcggtg atgagcgggg agcttgccga
    49141 ctgtttccca accaagatgg aggggatccg ctggatcgtg gggactgtac tggaggccat
    49201 ccccgatgcc ctcttctacg gtatcgacgc cgccttccac acccgccccg tccccgaact
    49261 cgcggcggcc aactggctgg cctcggccga tcacctccgg gaggactacc ccgacgccat
    49321 cctcctggat gtagggagca caaccgccga tatcatacca ctgaaccttt ttgacgatct
    49381 gaaggggctg accgataccc ggcggctcca gaagcactac ctcgtctata cggggctgct
    49441 ccggacaaat gttgcaaccc tcctccgctc ggtcacgctc gatgggacgg tgacccctgt
    49501 gagcaccgag tacttcgcct caagtgccga tgcccacctg gtgctcggcc agatcacccc
    49561 tgaggattac accgtccccg cgccggattc cggcgagaag accgtcgatg cggcgctccg
    49621 gaggctggcc agggttgtct gcgcagacct tgacgagata ggcagagagg gggcgctcga
    49681 gatcgcccgg cagttctggg aggtccagcg tacgatgatc gcagccgctc tggagcgcgt
    49741 tcaatccagg agcggtgcga cgggggtgat aacagccggg ataggcgcgg atctatttgc
    49801 ccgtgaactc ggcggtgtca cgctgaaggc agagatcggg gatgcagcgg acgcgctgcc
    49861 ggcatacgcg gtcagggagg tggcgctccg gtgaaccggt gggggctgag cctgctcctg
    49921 cttgcgggat cgcttgccat cacgctcata gcgctggcgt tcgggttccc gttcttcttt
    49981 ctcttcctat tcataccgtt gataccgttc tttgggcggc cggccggggt gaagcggtgt
    50041 ccggtctgcg gctgggaaac cacaggccgc gagcgcttct gcccctacga tgggaccccc
    50101 cttgcggttg agggtgaggg tggccaggtt cagcagggag agcggtaact gcagaccaaa
    50161 aacgtctgtt gcgggtatgg caccaaactc aaactcgcgg gaggcgtagt tcctgatcgt
    50221 cccccggtcg acgaccagcc gcatttcacg caggtattgc gccacccggt taaaaggcat
    50281 ctgctgcgcg aggagaacac acaggtcaac gatgggcgag gcaagacggg tgtcagggta
    50341 gaagggggcg tcggcgccgc agagagagcc gcaatcctgg cagcggaacc gtttcaccat
    50401 gaccctgatg gtgcgcctct tcccatcctc gattacgacg gcaaaccgtt tcgcccgcat
    50461 atcgtagccg ctcacatcgc cgccgcagtg cgggcaggaa ggcgggtcgg tgaaggccac
    50521 accgtccatc gagtcgagag cgttcaccac aaggtcaaca agcatcggtg atacaggcag
    50581 cctcttcttc acgaaagcaa ctctcccata aaggtatgca ggatctggta ggcgcccacc
    50641 actattaaca ccttccagtg gttccgacac cggttccgct gcggcagcgt ggagacgatg
    50701 ccgctccata acccacccgg cggggcgtcc gggatctgcc cgtggctgcg gtataagcat
    50761 aaacccgttt aaagaactgt tttaagaaaa tacggcatat aataaggcat acggcgcgct
    50821 tagtggattt taaccggtaa aaaagacatt gagagcaatt acagcctgtt ttaattcacc
    50881 atcgtggaac acgacggcag aaacccacat ttattcatca aaaatccgga agttaatcac
    50941 tataattaaa tatacctaac cctgtatttg tacgtatccg aggttttacc atggatttca
    51001 agtatgtaca gacaacatgc ccctactgcg gtacgggctg cagtttcaac ctcgtcgtga
    51061 gggatggaaa ggttgtcggc acacaacctt acgaccgctc acccgtcaac gagggaaagg
    51121 tatgcccgaa gggcacctac gctcacgagt tcgtgaacag ccccgaccgc ctcacaaagc
    51181 cgctcatcaa gaaggacggc aagtttgtcg aggcgacctg ggacgaggcg tatgacctga
    51241 tcgtggagaa gttcaaggcc tgcaagcccg acgagtttgc ggcgctcgca tcggcgcgtg
    51301 tctcgaacga ggagaactac ctgatgatga agtttgctcg cggcgtcatg aaatcccggc
    51361 acatcgacca ctgcgcccgg ttgtgccacg catccaccgt cgcgggcctt gcggcgtcgt
    51421 ttggctccgg tgctatgacc aactccatcc tcgacatcgc cgagtcaaag tgtgtctttg
    51481 tcatcgggtc caacacattt gagcagcacc cactcatcgg acgcaagatc gtccaggcaa
    51541 aaaagaacgg cgcaaaggtc atctacgccg acccgcgcta caccccgacc gccaggcagg
    51601 ccgacctcta catgcccttt atctcgggaa gcgatgtcgc gatcctgaat gggctgatgc
    51661 aggagataat caagaacggc tgggaggatc gggagttcat cgagaagcgg acaaaggact
    51721 ttgagaaact caaggaggtc gtcatgaaag aagcctacag ccttgagaat gtctctaaga
    51781 tctccggcat cccggttgag agtctcaaga cggcggccga atggctcggc accaacaagc
    51841 ccggcgcgat cctctactcg atgggcatca cccagcacac cgtcggcgtc gataacgtcc
    51901 ggtcggttgc aaacatccag atgctgcttg gcaaccttgg caagcccggc gccggcgtga
    51961 acgcgctccg cggccagaac aatgtccagg gcgcctgcga tatgggggcg ctgccaaacg
    52021 tattcacagc ctaccagaag gtcatcgatg aagccgccca caagaagttc gccgatgcct
    52081 ggggcttccc ggacgggatc tgtgagccaa aactcggcta cgaggtcacc accatgatga
    52141 acgtcctgac cgataaccct ggagagctca agtgcatgta catcatgggc gagaacccga
    52201 tgctctccga ccctgatatc aaccacgtca gagaggcgct cgagagcctg gatttcctgg
    52261 ttgtgcagga catcttcatg accgagacag gtgaactcgc ggacgtcatc ctcccggccg
    52321 cctgttacgc cgagcgtgac ggtacgcaga ccagcaccga gcggcgcgtc cagcgctgga
    52381 ggaaggcaca ggacccgccg ggcgaggcaa agcaggactg gcagatcata tgcgaactcg
    52441 ctgcccggat gggatacgcg gagcagttcc cgttccagag cgccgaggag atctttgagg
    52501 agatcaggcg cgtcacgccc tcctatgccg ggatgaccta cgagcggctc gaccggccag
    52561 aggcgctgca ctggccctgc cccaccgtgg agcatccggg aaccccgatc ctgcaccgtg
    52621 agaagttttc cagtcccgat gggcttggag tctttgcccc catcgagtgg aaaccgccgg
    52681 cggaggttcc cgacgaggag tatccgtttg tgttcacgac agggcgtatc ctctggcact
    52741 ggcacaccgg caccatgacc cgccgctccg cgaccctcga taccgaggtc ccaaccggct
    52801 ggatagagat caacacagag gatgcaaagg cgctcggcat caagaacggg gagagaatcc
    52861 gtgcggtcac ccgccgtggg tctgtcgagg tccccgcgaa ggtgacagac gacatcatga
    52921 agggtgtcat gttcatgccg ttccacttcc atgagtgcgc ggcgaacata ctgacgaaca
    52981 acgcactcga tccgatcgca aagatcccgg agttcaaggc ctgtgctgtg cgggtcgaga
    53041 agatcacgga ggcctgaaga tggcagcaaa aggcgatatg ttctacgcgt gggcggccga
    53101 cgccgcctgc caggagaggg gcgagtgcgg tggcgcggtc accgctctgt tgacgcacgc
    53161 gctcagatcc ggtatggtgg acgccgtcct cgccgtgaag agggggcagg acatctacga
    53221 tgccgtcccg accctgatca cagacccaga ggatatggcc gagacggcag gctcgctcca
    53281 ctgcgggacg ctcctcctct cgaaacttct caagaactac ctggacggcg ccgagaatat
    53341 gcgcatcggg atcacggtga agggctgcga cgcgatgggg ctctacgagc ttgcgaaacg
    53401 caaccaggtc aatctggaca acgtcctgat gatcggtgtc aactgcggcg gttctgtcag
    53461 ccccgtgacc gctcgtaaga tgatccgcga gaagttcggg gtcgacccgg acgatgttgt
    53521 caaggaagag attgacaagg gtcagttcat catccagacg aaagacggcc agcacaaggg
    53581 catctcgatg gatgaactcg aggacgaggg atacggccgc cgccccaact gccgccgctg
    53641 caagatgaag gtcccgcgcc aggcggacct tgcctgcggc aactggggtg tcataggcga
    53701 taaggccgga aaggcgacct
//
LOCUS       DAOSZX010000002        51403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_544_length_51403_cov_21.375945, whole genome shotgun sequence.
ACCESSION   DAOSZX010000002 DAOSZX010000000
VERSION     DAOSZX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 51403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51403
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_51403_cov_21.375945"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..673)
                     /locus_tag="PK196_00290"
     CDS             complement(116..673)
                     /locus_tag="PK196_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HON39894.1"
                     /translation="MPGLDTQVDDRERVEQDHPIFVIQKHAAKTLHYDFRLEVDGVLK
                     SWTVPKGPSLSPEEKRLAVPTEDHPIEYAEFEGVIPEGSYGAGAVLVWDRGTYRNLTE
                     RRGEKTGVAEGLRQGHISFWLDGTKIRGGYALTRFRTGKNEAWLLVKKNDDEADPGRN
                     PVVSEPRSVISGRTIEEIEAEEGDR"
     gene            complement(717..3008)
                     /locus_tag="PK196_00295"
     CDS             complement(717..3008)
                     /locus_tag="PK196_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON39895.1"
                     /translation="MSTEIAELLRTMGYSDAEISGLMESFPSTVIEYLRDFRFQEVRD
                     IIETLLYIYVAQNSASSRGEGEGVIEQSCYAYTSGPLFALTDIGLIESLSWHGTTLVR
                     PTATGEAIGRDLLKARLRRLDIQGLARETHEIVPVLLAGTVKGSFINKTFPSTPPRSD
                     ETFINFLLKNCPGLFEGCERFAARLKDAGCAVLAYSCDLDTGMIDGVVYTFPPEFAYI
                     LKEMLERIDAGVRDHYDMLLESFYSTFTVLRYLAFGEDYERIRGSRALRRELSRVLAS
                     LADVVYVLEGVPEDDGVDLARFIVRDRNLYEKRLRDLEHALMTEVAGKIVIDLQPMPA
                     AEPPGKIEDAHPFSEDAAIQSPAAGHREVGEEVWDDLAIPPEEGERIEDRAAAVAHPT
                     PAPLRNAPVTEPQRSGGLDLFLGYTGDGQRVNWSPGRLNNGHMIIVGGSGAGKTETIR
                     CIASELAAQSMPVLLIDFHGDMAGTGSDIRSYKIREGGQYYFNPLELDPGIDEITPLR
                     ATSDFVDAISINFPTLGIQQRRKLKNIIKDCYRTAGITADTATWTRVLDFDDIEGGIM
                     SCEDETIPAYLEDIFDYNLFSGEEKISIPAILSGGITHINLNALPESLRYLFADLFLR
                     RIYYTLQAMGEIPRGTDDERAKFRLFVIVDEAKLLVSQKSGSKTAIKAVLNKYATEMR
                     KFGVSLILASQLIAHFNEEILANIAVKFCMRSENKKQAQENARFFEVSEKDLLNFQPG
                     EGILIIGSEKMNIRVVPTSERKT"
     assembly_gap    3236..3335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3438..3923)
                     /locus_tag="PK196_00300"
     CDS             complement(3438..3923)
                     /locus_tag="PK196_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 family protein"
                     /protein_id="HON39896.1"
                     /translation="MELKEQLSGVPGMLRPFKGFLRDSGLNEGDQVVYYGCPGTCTPF
                     IELLGFAVRDLPIEQVYVPYVNEAAAKVIRPVEGVGMQVSAESAVKVDPKVIVLMGGL
                     SMPGVPVTKEAVRGAVATHPGAMIVGVCFMQMFEKMGWVEMFDFDLLIDASINPVRVW
                     Q"
     gene            complement(3979..4326)
                     /locus_tag="PK196_00305"
     CDS             complement(3979..4326)
                     /locus_tag="PK196_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39897.1"
                     /translation="MARELDEKDLSILRKLAPEYSGILCPGSGHEFHSILPPVSNHIA
                     RDDKDFAERVARLSEEDWAYLTDLILSGREDLGCVPEEDIETILAHIGERVSEKAAER
                     VRRLYHLSGCGVL"
     gene            complement(4595..5377)
                     /locus_tag="PK196_00310"
     CDS             complement(4595..5377)
                     /locus_tag="PK196_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39898.1"
                     /translation="MDLLSSGDAGDLAAYLAAESSLAGPRTNQDLTEAFARAIREFAV
                     ADREDRRILWDLCVELACISPEDAPTDDPHEFLSVCGVRGIAAMGTISPGCVKAALRK
                     LAESAEDPRWRIRDAVAIGLRDLLARHRDVTVSELNGWVEGGSWSAMQTVAAGVADAD
                     VLREPDLAEAALRLHRKIMIRVYTAGERESEDFQALRKTLGCTLSRVVAALPSSGFEY
                     LQQVSTLDDQEIRWIVRENLKDNHLAGHYPETVRHIQAQIGE"
     gene            complement(5465..5950)
                     /locus_tag="PK196_00315"
     CDS             complement(5465..5950)
                     /locus_tag="PK196_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HON39899.1"
                     /translation="MAEDRLKIVVFGSFNAGKSSFIQALDPHSRHIEASSDDGPTTVA
                     LDFGRLKVNGRAVYLFGTPGQERFEFVRQVLSRGMDGAIIMVDATKGVDAMTRDLYAR
                     LKTLEVPLAFMANKCDCPDAMPDAICRDLHGEMVFPISALNSEDVHAALETFVETLSR
                     R"
     gene            6166..6516
                     /locus_tag="PK196_00320"
     CDS             6166..6516
                     /locus_tag="PK196_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39900.1"
                     /translation="MGPDEMDPVVLEIMAVLDSLFLAERQARFQVSALEVQDYPFAAT
                     FEMVRDTESDAAIEEALSGFGFEQHTLDDDAELWISDELGLMVFLFFTTPDGRSYTYR
                     IVRFEVAGEDEISL"
     gene            6631..7161
                     /locus_tag="PK196_00325"
     CDS             6631..7161
                     /locus_tag="PK196_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON39901.1"
                     /translation="MERSVKRLMEDRDKRREENIEKYIEQLSDESTPYRLRAVEALGT
                     CSDPRVVEPLIAALDDPENEVRWVAAQALGKVRDPRAVEPLLSLLADHDRWVRRGAAW
                     SLGEIGDPRAVEPILPLLADKKKDVRTAAADALGKLRDSRAAAALSAALEDEVEPEVR
                     TAIRRALREITGEAEP"
     gene            complement(7456..8913)
                     /gene="ppcA"
                     /locus_tag="PK196_00330"
     CDS             complement(7456..8913)
                     /gene="ppcA"
                     /locus_tag="PK196_00330"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HON39902.1"
                     /translation="MIRIPQCMSTQHPDNVNLPFFAESPELGGDDEVQEAYYAYSHLG
                     CSEQMWDCEGKEVDNFVVRKLLSRYETFFREKRLGRDVFLTMRVPNPEVESAEAKILL
                     ETLESIPRSFDTACLFYGEECSPIFEVILPMTTSHLAIDNIYRYYRDVVVGKQERRLR
                     EGGPTIADWIGRFRPDRINVIPLFEDMARMLDAHNVVRRYLQDKDIADQRVFLARSDP
                     AMNYGLISAVLLNKIALQNLQGVSEEAGAGIHPIIGVGSAPFRGGLTPLTVDRVAAEY
                     PSVRTFTVQSAFKYDYQLEDVQNAIRVLEERQPAKPTKIEEDAALDIIWRYTRAYERW
                     LGPFAPLINRVARYVPERRKRKLHIGLFGYARNTGGITLPRAIKFTAAFYSIGLPPEI
                     LGLNVLDDADIEFIRKVYVNFDEDLRAACRFLNTETGFVPPDLAARVTDLVDVVQDEE
                     HARLTTAISRALRENETGGLTPLILEAANRRRFLG"
     gene            complement(9231..11423)
                     /locus_tag="PK196_00335"
     CDS             complement(9231..11423)
                     /locus_tag="PK196_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HON39903.1"
                     /translation="MTRRKKMVERVTELMDKPEQIRNIGIVAHIDHGKTTLSDNLLAG
                     AGMISEELAGRQLFLDSDEEEQVRGITIDASNVSMVHEYDGKEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVVVDAVEGTMPQTETVLRQALKEGVRPVLFINKVDRLINELKVN
                     EQEMQIRLARVIDKVNKLIKGMNEKMYNEGWKLDAAKGTVAFGSALYKWAVSIPFMKK
                     SGVSFKEVYEKCNSGDMKWLAKNSPLHSVLLDMVVKHLPNPIEAQHRRIPIIWHGDYS
                     TPEGKAMINCDPNGPVCLMVTDISFDPHAGEVATGRLFAGTLRRGTECYIMGTAKRVN
                     RIAQVGIFMGAERVEVDLLPAGNIAAVTGLKDAIVGSTVTTLIDMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTVQVSINEETGEHLISGMGELHLEIITGRI
                     KRDKGVDIITSPPIVVYRETITGRAGPVEGKSPNRHNRFYIELEPMDPAIVKLIHDGE
                     VSMNQQALERRDILVAAGMDKEEAKNVRAIEGTNMFIDMTKGIQYLNETMELILEGWR
                     EALRGGPLADEQVQNLKVRLVDVKLHEDAIHRGPAQVIPAVRSAVKAGMLMAGDSLLE
                     PIQKIQITVPADLMGAATSQIQGRRGQIFDMQSEGDTVTVIGKAPVAELFGFAGDIRS
                     ATEGRAMWSTEFAGFELVPAGLVEEVVRSIRRRKGLKEQIPRPEDYLS"
     gene            complement(11453..12043)
                     /locus_tag="PK196_00340"
     CDS             complement(11453..12043)
                     /locus_tag="PK196_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HON39904.1"
                     /translation="MTEAETEANVQQLLFNRWDMSEVEIRDPSLARYVNLHTMIVPHT
                     CGKLAGQQFNKSNMLIVERLINKLMQTEHNTGKKELAIRIVRDAFEIVYQKTRKNPVQ
                     VLVDAVANAGPREETVRLKYGGINVPKSVDTAPQRRVDTALRFITDGVYQASHKKKKS
                     VSEALAEELIAAANGDTRSYAISKKEERERIAKAAR"
     gene            complement(12050..12478)
                     /locus_tag="PK196_00345"
     CDS             complement(12050..12478)
                     /locus_tag="PK196_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HON39905.1"
                     /translation="MGNGKFAARKLKRDSKKMRWHDPVYARRQLGLDVKSDPLEGAPQ
                     ARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRYVVTKVNNVSLREMVIGRKEKPRR"
     gene            12653..13066
                     /locus_tag="PK196_00350"
     CDS             12653..13066
                     /locus_tag="PK196_00350"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HON39906.1"
                     /translation="MRARIYKEVFFEATHRLIHYRGKCFRLHGHQWRVEVWIEGTADE
                     RTGIVLDYNCIKEVVGRFDHQVILNEDDPMAACIETFHPVVTTRGDPTSETLAGVIAD
                     MIDAEAARQGSDARVAKIRVWESTSCYAERTYDRQ"
     gene            13053..13661
                     /locus_tag="PK196_00355"
     CDS             13053..13661
                     /locus_tag="PK196_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON39907.1"
                     /translation="MIVSEIFRSLQGEGKNQGRPCTFVRLAGCNLRCAWCDTSYAWNE
                     GEEMSVGEVLDRVWLLRGAYICITGGEPLLQREDLLELLKKFKLHGYSVEIETNGSLD
                     FREMQPYASICMDVKCPSSGERSDLSLLSAITAHDSVKFVVADMDDLLYARAVMGRCR
                     IGGEVFVSPVEGSDCRAIADYIVSENLPVRFQVQLHKVLGMR"
     gene            13662..14327
                     /gene="queC"
                     /locus_tag="PK196_00360"
     CDS             13662..14327
                     /gene="queC"
                     /locus_tag="PK196_00360"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HON39908.1"
                     /translation="MKAVCLLSGGMDSSTLAYVAKDMGYDIIALHFTYGQRTEEKERE
                     CALLIARKLEAIEFVEIGLEHFRKIGASSLTDPSIPIEDYAGEKGGVPTTYVPFRNAN
                     LLAIATSLAEARRAEAIFIGVQTGDYPGYPDCRPEFIEAFQRVIDLGTAADPRITLMT
                     PFVRMTKADIVRVGLALGVPYELTWSCYRSSGRACGVCSSCHYRREAFRELGLEDPIE
                     YEA"
     gene            14330..14824
                     /locus_tag="PK196_00365"
     CDS             14330..14824
                     /locus_tag="PK196_00365"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HON39909.1"
                     /translation="MEIYSGKRLTVEKKVVTLPDGEKKERVIVHPGGAVAILPVEGDV
                     CHLIRQYRFAIDDYILEAPAGTIDEGEKPEETAYRELIEETGMKAERFIPKGWIYPSP
                     GYTDERIWLFLAEGLSPSSEYRRDDDEFIEVLRVPLSALPGMIANGTIIDAKTICLVC
                     RCLG"
     gene            14921..16192
                     /gene="prf1"
                     /locus_tag="PK196_00370"
     CDS             14921..16192
                     /gene="prf1"
                     /locus_tag="PK196_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HON39910.1"
                     /translation="MVETQEMETARKRYEFKKTLERLAEKEGSGTELITLYIPPDKQI
                     SDVTAQLRDEFGQCSNIKSKQTRTNVQSAISSILSRLKYYKRPPENGLAIFCGTINVG
                     GDRTDLQCEIIEPPEPLNTYMYRCSSTFELEPLQQMLEEKEVYGLIVIDRREAYIGFL
                     RGNRIEPVRGVTSTVPGKQRKGGQSSVRFQRLRLIAINEFYKKVGDLASEIFLGEKDF
                     FERFKGVLIGGPTPTKEEFEAGGYLHHEVQKRIIGLFDVSYTNESGLAELVENAADAL
                     KGLEVIKEKTVMNRFLQELVKDDGAAAYGEESVRKNLELGAVDTLLLSDKLRRARLKL
                     ACPNCDYTEERTVSVEPGKHIKDLDLGTCPNDTSPLYVAEESDIIDELTALADQSNTT
                     VKIISDDFEEGSMLYNAFGGIAAILRYRTGY"
     gene            16192..17856
                     /gene="argS"
                     /locus_tag="PK196_00375"
     CDS             16192..17856
                     /gene="argS"
                     /locus_tag="PK196_00375"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HON39911.1"
                     /translation="MYQEKYHHIERMLRACTGEEDVLLTAGGDHADLASTVAFKLAKK
                     EKRPPQEIAADIAKKLGKNPELGDIQVEAAGPYLNFRFGPTLLAEAVREAVEPGYGSR
                     PRRSSRVVLEHTSANPNGPLHVGHIRNTVIGDSLARAFRKAGYPLEVQYYLNDMGRQI
                     AIVSWGFDHLDIPRGKGEKEDHYVARVYIAANREIEKNPEITHEIDLLMQKVERGDPV
                     AVKKFRSAVSLCLEGIRATLAALNAYHDRFVWESDFVRIGDVDRVIERVRRLPQAVED
                     ETLWVDLSEFGFEKRYILRRSDGTSVYSTRDLAYHIWKGRNYDRLIDVLGADHKLIGA
                     QLQATLKLLGEKPPEIVFFEFVSLPEGSMSTRAGKFVSADELIEEVAAKAFEEVTARR
                     PELPEGERCAIAKSVAIAAVRYDIVKVSPEKSTVFDWKEALDFERQSGPYIQYAHARA
                     CSILEKAGGFEPAYHYETDHEIALARHLARFPAVIEQVVAELRPHLLAAYARDLADLF
                     NAFYHYDPVLKSEGETRNSRLTLVDAVRNTLKESLETLGIDALRSM"
     gene            17837..18730
                     /gene="twy1"
                     /locus_tag="PK196_00380"
     CDS             17837..18730
                     /gene="twy1"
                     /locus_tag="PK196_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HON39912.1"
                     /translation="MHSEACNVLRRQGYQFITPASSAAVKPCMWCKRALKGGEMCYKA
                     QFYGIESHRCVQMTPTLRCNQRCLFCWRSFEHEVVEEEECPPEVIITNLRRAQKKALA
                     GYKVSPYVTPERFAEALNPTMVAISLSGEPTCYSRLPELVDGLNAEGYTTFLVSNGTR
                     PEVLGRCRPYQTYVSLDAPDRETYLRLCRPKEDYWDLVLESLAGLAGRRSAIRTTLVR
                     GYNDFAPERYAAIYQGSGARFVEVKGYMYLGYSRNRLLREQMPEHAEIREFSEMVSQH
                     SDYFIKDESPLSRVVCLERDV"
     gene            18727..20319
                     /gene="sepS"
                     /locus_tag="PK196_00385"
     CDS             18727..20319
                     /gene="sepS"
                     /locus_tag="PK196_00385"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HON39913.1"
                     /translation="MRFDVEEFKKRAREDFEHAWYEGPSVLTPPGVSGRYPRLRYARA
                     REHPIFDIVERLRETYLAMGFDEAMNPLIVEESDIYRQFGPEAMAVLDRVFYLGGLPR
                     PNVGIARKQIEEIEAILGRALTAGTEERLRETLHGYKKGKIDGDDLVHELAAVLEADD
                     AAVVHILDAVFPEFRDLAPESSRNTLRSHMTSGWFLTLSSIWDRQRLPLRLFSVDRCF
                     RREQEEGPTRLMAYHSASCVVAGEDVTLEEGKAVSEALLSAFGFTEFRFQPDEKRSKY
                     YMPGTQTEVYARHPSLGWVEVATFGLYSPSALAEYGIGVPVMNLGLGVERLAMIVYQS
                     NDIRQLTYPQFFPQEVSDREIAGAIHLREEPASVAGRRMAEGIRATAAEHAAAPGPCA
                     FVAWSGEIAGREVEVVVEETESNTKLCGPACLNEVFVHNGSVLGVPDTAKWATVRQEG
                     VSTGITYLDAVAGLAAARIEEAARCGEEVRVQVKMAKLPSDVNLRIEDYVMRYITDHN
                     KKVDLRGPVFLTVRSVIRKPGG"
     gene            21042..21428
                     /locus_tag="PK196_00390"
     CDS             21042..21428
                     /locus_tag="PK196_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39914.1"
                     /translation="MKRTYAVKIGVGVILAAAGIILPFLVDGMESLSSVLVAIGLVTV
                     AVTGVQHWRCSDDLEKDERTQKLGAYAISYSWLLTLVFLAILFWVDYLGLLVLSVQAV
                     LVSAILLMGLSARLFQWYLFRQGDVA"
     gene            21442..21648
                     /locus_tag="PK196_00395"
     CDS             21442..21648
                     /locus_tag="PK196_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON39915.1"
                     /translation="MKTRIKEFRAKAGMTQEELARQVGVRRETIVFLEKGKYNPSLKL
                     AYRVAKALDSTIEEVFIFDEEDPA"
     gene            complement(21676..22722)
                     /locus_tag="PK196_00400"
     CDS             complement(21676..22722)
                     /locus_tag="PK196_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HON39916.1"
                     /translation="MRDLQKGHLHHSGYQEARRFRANLIERCRGLAIEDVFNGREVRC
                     SGGRCYVIDSRSPIPLAVRDRERAAAAILGDISLIQGIGPATRQRLEERGYRTVPDLL
                     GHPRYHQDASRLLDLVARGETRELVNWIERRHRQSDPLLLEASRFHAPEDFLFLDIET
                     LGLSSRPIILIGLGRVERGSIVVRQHLPRSIKEETAALTAILPDLEAERAALVTFNGR
                     AFDLPYIRDRLARHGVPAALDLPHFDVLQFARRCWGGRLSSCRLGSLETAVLNITRTD
                     DLPGAMVPEFYETYMRTGNPGPLIPIVEHNRQDIISLARLFALLRGDDRTAATIEAPI
                     PEMGDERGEIKPQS"
     gene            23025..>23311
                     /locus_tag="PK196_00405"
     CDS             23025..>23311
                     /locus_tag="PK196_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HON39917.1"
                     /translation="MNIGAVLIDIDGVLHVGDRPVEGAGRALRELDRRGIPYRSVSST
                     TRSSRRSVAQRLHRLGYEIPEPWIVTAPAAAAVDLRESGRTRCCLLTTPDAR"
     assembly_gap    23312..23411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23503..24603
                     /locus_tag="PK196_00410"
     CDS             23503..24603
                     /locus_tag="PK196_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39918.1"
                     /translation="MIKRILFLVLLAAAIGGGCLGFFLRPVFVPPAIVPLEGASLNQT
                     PVYTFAFEDGMETIRIDPDPAVYAGAKAADRSLHLYTDMPEEEWIPIYYLAFINEPHQ
                     EPFYADLLGALREIRDRRGLDDDRYLELIAVFVQSIPYETDASVTEPKFPIETFVDAA
                     GDCDDKSLLLAGLLAREGYGVSLLYFGDENHMAVGVKSAGCGYRNTSFAYIETTNVSY
                     VGIPPATLSGGATLSSDPLVIPVGEGERSYTACNQVQTIERALSALRDRAAVIETELE
                     ARGREIEAMRDALDALNARMTPLLRAGDISEYNRLVQDYNRMAGDYNQAVDAYNALLD
                     EAERVVDLHNDLAAGAHDRPASYIMARAYLCG"
     gene            complement(24588..24782)
                     /locus_tag="PK196_00415"
     CDS             complement(24588..24782)
                     /locus_tag="PK196_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39919.1"
                     /translation="MERSELTTFACLAERAARYPTITRRSQIRKEVYIAADGAVGRPS
                     PGAERRTQPFSGLNECHPQR"
     gene            25069..25803
                     /locus_tag="PK196_00420"
     CDS             25069..25803
                     /locus_tag="PK196_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON39920.1"
                     /translation="MLDISDLHVEVDGTEVLHDINLHIGWGETHVLMGPNGSGKSTLL
                     KTIMGFGGYTITSGSIIFKGQDITRMPIHERAHLGIGMMFQNPPAISGLKLGKLLTAI
                     SGLKEDEIEALARSVNMGQFLNRDINVGFSGGEIKRSEVLQLKVQQPDFLMLDEPESG
                     VDLENINLMGREIATLLEKDVRIVNRRRSGLIITHTGYILDYVDADQGHVIIEGQIRC
                     HGNPREILRLIKEKGYGECLRCKQIS"
     gene            25809..26900
                     /locus_tag="PK196_00425"
     CDS             25809..26900
                     /locus_tag="PK196_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HON39921.1"
                     /translation="MEHLSEKDRERLALTGLEVEMRNRCGSFFQIDQNVVQTTCMAEG
                     VEMLPIKAALEKYDWVKDYYWKAVPRDKDKYTRYVADQENPQGLVVIAHPGTKIEMPL
                     QACLFLREGPVQHVHNIFIAQEDSEIHIISGCASSWQKEHGAHIGVTEIYVGKGAKVT
                     STMIHNWNRGIAVFPRSATIVEEGGLFLSNYVCMQPVRKVSMYPTASLVGDNAVARFS
                     SIVVATPGSTLDLGSRAILGAEHTSAELVTRAITTGGTIISRGHILGAKDNTRGHIEC
                     RGLILKDGIIHAIPEIEGTITGTELSHEAAVGKIARHEIEYLMARGLSEEEATATIIR
                     GFLDVKISGLPAVLQAQIDAAIDKAESGF"
     gene            26916..27581
                     /locus_tag="PK196_00430"
     CDS             26916..27581
                     /locus_tag="PK196_00430"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HON39922.1"
                     /translation="MTEIDPFAGAREAMVEFQIRARGVSDPRVLAAMRKIPRHLFVPE
                     ELRHAAYDDRPLPIGEGQTISQPYIVAVMTEQLELSPGDRVLEIGTGSGYQAAILAEL
                     AGSVISIERLPRVAEKARQNLARAGVTGVEVVVGDGTKGYPPAAPYDAIMVTAAAPEI
                     PKPLIDQMAEGGRLVAPVGSRDYQELIKLVKRGGGIETIPLGGVCFVPLIGQFGWQGG
                     YSS"
     gene            27557..28192
                     /locus_tag="PK196_00435"
     CDS             27557..28192
                     /locus_tag="PK196_00435"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HON39923.1"
                     /translation="MAGRLLLVTVHDITRVLSRDAVVYPGDVRPRFREIDNGQYRVTE
                     MTLGSHTGTHIDAPSHYIEGGRTVDEIPPGVLVGPARVLDCSDAGEVIGPGRLLGRLD
                     GVRTLLLKTWFSGLREFDPGYPSLSMEAADLIIAAGITCIGTDAPSIEGFSGDGSVHR
                     RLLGNGAVILELLDLSGVAEGEYFMAALPLLLRGGDGSPLRALLWDAEDEV"
     gene            28189..28794
                     /locus_tag="PK196_00440"
     CDS             28189..28794
                     /locus_tag="PK196_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39924.1"
                     /translation="MSLWHDAVERLEKILAGSGCEDGIVTLGVNYDADRCQYPKGVCM
                     EAQFGGRSGQFVTNEPTRARTRISFMFGAPLATTKQRAAAGAIINAVSAFLCLTRRLQ
                     PCTKECHGPCLEALSREVAGKRVYLLGPMPLLVRALEAQVADSPEEADLILVAGDGMA
                     TDAGVACIDGYRGRKRMIFIGPSTAGISGLLDLEHWCPYGR"
     gene            28830..30086
                     /locus_tag="PK196_00445"
     CDS             28830..30086
                     /locus_tag="PK196_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase/phosphoglucosamine mutase"
                     /protein_id="HON39925.1"
                     /translation="MLFGSSGIRQEFGPDLVDLAFRIGSAAAAGASEIALGRDTRTTS
                     ELLEHSVVAGMLATGATVYSCGIAPTPTVAYATGSADAGCMITASHNPEPYNGVKLLN
                     PDGSSFTVRQQAALEEALNGTHWKGWDEMGRLLPIDAIDVHREAILDKVSLSHPIRAV
                     LDCGNGAGSVTTPDLLAEAGVALTGLNCNVAGKFARPSEPLESNLPYIGEIVRRRGAA
                     CAVVHDGDADRMMAFDERGRYIDGDRLLMLFVKYLDCKRVVTTVDASMAIEEIADVRR
                     TPVGDSFVSEELISWGDFGGEPSGSWIFPGHSLCPDGVYAAALLCEIASECSIAEELD
                     RMPRYTLLRRSCPVPDPRRVMAAMGAEEPAHGIRFEDEDGWYLVRASGTEPKIRITAE
                     GRTPEKAEEMLEAASAALKRARRSLE"
     gene            30094..31293
                     /locus_tag="PK196_00450"
     CDS             30094..31293
                     /locus_tag="PK196_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HON39926.1"
                     /translation="MQCVVLAAGEGKRMRPLTARRPKVMLPIANRPMIEHLITAARDA
                     GITGFILVVGYCEREIRNHFGDGSGLGVKITYVTQRRQLGTADALRAASGMVNDRFLL
                     LNGDMILRSDDIRSLCRMEAPCMGIHETDHPQDYGVVTVEADQITGLEEKSEEPKSNL
                     INAGAYIFDPDIFDLLSGVGVSERGEFELTDALASYIEEGRLRGYLLDYWLDLGHPWD
                     LLDANAEILSSMHHEKHGVIEPGCSVPETVSIGRGTVIRAGTYIEGSCIIGENCVIGP
                     HAYIRGSTAIGDGCHIGHATEIKNSIIMSATKVPHFNYIGDSIIGSGCNFGAGTKVAN
                     LRHDNGAVKVCGKSTGRRKFGAIIGDDVLFGINCSVNVGTSIGSGTRVSPHSFVDGCI
                     QENSVIR"
     gene            31298..32449
                     /locus_tag="PK196_00455"
     CDS             31298..32449
                     /locus_tag="PK196_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HON39927.1"
                     /translation="MQAVILAAGEGSRLRPLTRSKPKALIPVANRPIIDYVIDALLEN
                     GIREIVVVVGYRKEEVIQHLNSLDAPLQVVVQERQLGTGDALRAAEPEIRGDFLVLPG
                     DNYINAGSIARIKGVRNAMLVAEHPNPSNFGVVVIRSGIVREIVEKPEDAPTFTVSTG
                     VYSFTPEIFSYLHTNEIPDAVGAMIADGRQIRAIPADEWQDAIYPWDLLKLNTRMLRG
                     IQPGIAGTVDASVVRRGVVQIGKGTTVGPNTVIYGPVNIGSNCNIGPNTVIMPDTSIG
                     DRVVLEPFTYVADSLIMSDVTVGSHSRIVSAVLGQGCILADHITTTPSASLIEVGGRV
                     QKEEFGAVLGEGVRAAPFTTFKNCIVGNNVSIVKGRMLAGRIEDGTRVM"
     gene            32450..34189
                     /gene="glmS"
                     /locus_tag="PK196_00460"
     CDS             32450..34189
                     /gene="glmS"
                     /locus_tag="PK196_00460"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HON39928.1"
                     /translation="MCGIVGYTGRRAATPILVRGLKRLEYRGYDSFGIATVGSAIEVY
                     KKAGRIPDSGVVPVDLAGCTGIGHTRWATHGEPNEINAHPHVDCGGRIAVVHNGVIEN
                     YAELKRNLQERGHTFRSETDTEVIAHLIEERYDGDLLAAVNAVLPLLRGSYAILVIAC
                     DSQRIVAARDASPLVLGIGDDEVFAASDMTPLLEYTERVIFLEDGDVADLTPCRCTIY
                     SGGRKVERPVELINWCLEDTRKGGFPHYMLKEIYEQPQSFYETLRAGVDERVRKLVME
                     ADEITLVACGTSYHTTLAFRYLAESLCGIPVRVEMGSEFKYFTPPLHGLVIAVSQSGE
                     TADTIAALKMAKARNCPTLAITNMQGSTVTRIADETMLMRAGPEIGVAATKSYMAQLA
                     ALMQILNIRCGGEFDDALSHAHLAIGEVLIQDIQDAVDLCSRAEHVFFVGRGPFYAVA
                     LEGALKMKEISYVHAEGYAAGELKHGPFALLTPETPVVAICTPGPTYGVMVSNIKEMK
                     ARRAPIIAIGVAGDTELPEIVDIFIPIPNTHLLVQVLTASVVLQLLAYHTACALDRDV
                     DKPRNLAKSVTVE"
     gene            34186..34785
                     /locus_tag="PK196_00465"
     CDS             34186..34785
                     /locus_tag="PK196_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HON39929.1"
                     /translation="MIEYGRNALGDGATIFEPVTIGFPSRDRMGLEDYPGAAIGKNAI
                     LRSGTIIYCDVRIGDAFQTGHNVLIREKTVIGDRVAVGTAAVVEGDCTIGDDVRIQSL
                     VYVPTGARIGDRVFIGPNAVLTNDRYPPGPRESLRGPVLGDDAVIGANATILPGVRVG
                     EGAFVAAGAVVTRDVPPEMLAVGAPARFRALPAEARRCR"
     gene            34782..35891
                     /locus_tag="PK196_00470"
     CDS             34782..35891
                     /locus_tag="PK196_00470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HON39930.1"
                     /translation="MMIPVARPVVGEEEISAVAEVMRSGMLARGSVVAEFESRFADYC
                     GAAHAIGVNSGTAALHAALLAAGIRPGDSVIVPAFTFFATASSVSMCGATPLFADVDP
                     RTFNIDPASVEALISPDTRAVIGVHLFGQPFDLDAVRALCDDYGLMLIEDAAQAHGAE
                     YHGRRTGSLADIGCFSFYPTKNMTTGEGGMVTTDDERIAERVRLFIDHGQSRKYLHTV
                     IGYNYRMTNIAAAIGMVQVERLEAFNERRIENARYLDRHLAGTGLVTPHVAPGVRHVY
                     HQYVVKLPAGYPLAREAFISALAERGVGTAVHYPIPLHRQPVYEGAGKTASCPVSEDL
                     AASVVSLPVHPSVTDEERAYICDAVGGFQALKQGQ"
     gene            35897..37039
                     /locus_tag="PK196_00475"
     CDS             35897..37039
                     /locus_tag="PK196_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON39931.1"
                     /translation="MRILLVSTQDYIHHPIPSRHHNIFEELAKRHEVHVPHFHVSRGK
                     ERETLLHVHEATWLPIKNPILHYTLNAPCHYRVISSIIRDHEIDVAVGAHVLAGTAMV
                     RAAERYGVPVVFDLKDWFPDSAAAYYKNPAIKWVLREGVLAITRYNLDHSDLITTVSP
                     GLVEKLRSYGYEAELITNGVNTDIFHPMDGSAMRSALGIAPDAFVLGFAGAIEHWYAL
                     DEVIRLFPEVLKRHPNAELLIVGGSLFTGYMDELQALAARLGVSGKVHFTGTVDYNDL
                     PRCIAPMDLCLIPLSPPQWVDIALPNKYFEYSACGKPILSTPIPDMLRMGGDHITVYR
                     DGQEFLEGVDEAVMNPRCCTIPIEEHAWKKKAEAFERIFTRLTGKT"
     gene            37131..39776
                     /locus_tag="PK196_00480"
     CDS             37131..39776
                     /locus_tag="PK196_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HON39932.1"
                     /translation="MDIKKYQPYIIIGLIVILALLTLWTRGIPAEGLVTDEGVNLLGN
                     DPWYNLRQVEQTVANFPAYAWFDAMTLYPTGDMIYWGPLFIEIISVFCLLAGATTRPE
                     IMLVASWVPPLMAVVMVPVVYLLARKIADWKTGLIAAGLIAVIGSNYAYRSLFGFVDH
                     HIAETLFSTIFVLAYVTALLAARGATISRANLQGLKIPAITAIIAGVAYLLGYFTMPT
                     MILFALIVAVFTLVQFILDYFQGRSSNYLVFVNAVVFIVVIIGTVAFGFPHPGLDLSR
                     YTIGHVIASAALIAGTAALYGLSVFLKERPKYYFPLALLAIAAVVTAVLYVALPELYN
                     LLISSLISFFGEQPVTTTVQEARAWSFDAAWATFHWGLLLAAGGAATLIWQNRKEVNP
                     AHTFVLVWTAIILGSTAAHVRYEYYLAVNIALLSAVFAGAVLGATWKEVALLLRKGSA
                     DSARPAAGRAEKREEPPKKKSKKGARAADAPKAKSRKRDQPDYLKVGGFVLVAALTLL
                     FAGTSFQGNLDLANSAKYSGMDSQWMEALEWMGENTPDPGVDYYAIYDPETFTYPEES
                     YGVMSWWDYGHWITFISKRIPNNNPFQHGVAGPNGSAVYLVSTSEEAANRILDNIGTR
                     YVVTDYQVVMGKFHAPATWADPNDTGGLRFQPVFFVPMGSGSAGYQAMQFYNQDYYMT
                     MVSRLHNFDGSMAEPTTGSVLYVEYAEPEMANASYPVITSAQEMNATAAAAAAAAYNQ
                     NAPAGSHAAVLNLYYEYQADSVLHPLERVPALKHYRLVHETPQNIYGNVGEAGPDLKA
                     VKIFEYVPGAAIRGEGVIEVPIATNTGREFVYRQESENGLFVVPYATSGWSGEVRATG
                     PYRIAGTGQTFDVTEEDIQQGRTIN"
     gene            39782..40750
                     /locus_tag="PK196_00485"
     CDS             39782..40750
                     /locus_tag="PK196_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HON39933.1"
                     /translation="MRYSIIYGDIFTRHDIEAHPESAARLRAALDCIPPSARWRPPIP
                     ACEADLERVHDPHYIRWVREMATGACFLDANTYVTCHSFEAAAHAAGSTIAAAERTLD
                     GEHCFALVRPPGHHAEPDRAMGFCIFNNAAVAAAKALLSLDRVAILDWDLHHGNGTQT
                     IFYGSDRVLFCSVHQENSFPWTGWVDEIGTGAGRGYNLNAPLAAGSTLADYALVFEEV
                     FIPAIVRFRPDLLIVSAGQDALADDGHGGMKLEPDDYGVLAGMLIDGTDLPLALTLEG
                     GYGPSAGKAVGAIFQALAGRRFEPAEKPVHRSTERVVRILKRVQFC"
     gene            complement(40775..41968)
                     /locus_tag="PK196_00490"
     CDS             complement(40775..41968)
                     /locus_tag="PK196_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HON39934.1"
                     /translation="MPITIRPVEKVYFDGGHRSRSPGETDAVVKPLMSGIGVTEITDI
                     TPLDRTGIPVFAAVRPGAARGAVRVYTGAGKEAVQARVSAMMAAIERCCAEYHGDHMD
                     YASYEEIGPARALHPEDLILPRELEQGEVVHWTPARDILNDEDLYVPSNAVFHPYDSL
                     GLTTPLFRSDTNGLAAGNVMEEAIFHALLEVIERDALSIADAGRNPGRRLTIEKDCAA
                     RGLLERFEQNGIEIHLWLLDGRTEIPTVAATADDTVTRDPAMILTGSASHLSPEAAAF
                     QALTEVAKRRGSILHGEPGDIRREAVIRKAGYERLKRINRIWFGNADAIDLADIPDMS
                     TGRFDLDIRRVLDEVAPHTDRVCVCDLTRTAIPVVRVIIPGFEVSCMDPRRRRSSRGG
                     GLTLP"
     gene            42025..42315
                     /locus_tag="PK196_00495"
     CDS             42025..42315
                     /locus_tag="PK196_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HON39935.1"
                     /translation="MSTDDIAVGAVVRYPRTGTTGKVVKIEEIDGREYAEIDATGLYY
                     LLDELIRTELTEVEARKKERSLEDYMREHREIQEQLEKVWEVGTDQSCEGGG"
     gene            complement(42312..42734)
                     /gene="nikR"
                     /locus_tag="PK196_00500"
     CDS             complement(42312..42734)
                     /gene="nikR"
                     /locus_tag="PK196_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HON39936.1"
                     /translation="MSGDTELSRIGISLPKSLLDKFDEILALRGYSSRSEGIRDSIRN
                     YITHYQWISDVKGERQGVITMVYDHDQRGLLQTLIDIQHQYTGNIQASLHSHVTHSKC
                     LEVILVRGDGTILKDITEKLMAQKGVEAVKLTTIPIEG"
     gene            42946..44181
                     /locus_tag="PK196_00505"
     CDS             42946..44181
                     /locus_tag="PK196_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HON39937.1"
                     /translation="MRDVFIPGVNIGLVGHVDHGKTTLVSALTGTWTDRHSEEIKRGI
                     SIRLGYADATFYRCEKCKGSDAYTTSAECPNCGGKAVPLRTVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANESCPQPQTKEHLMALELIGINRIVIVQNKIDVVTQAEALEHY
                     KQIKRFIKGTIAENAPIIPVSAQKGVNIGALIQTLDEVIPEPERNPDLDPLLLIARSF
                     DINRPGCNWRDVKGGVIGGSLIRGVLREGDEIEIRPGRQLQVENRTKWEPITTKITSI
                     NAGRINVTEASPGGLLGIATKLDPALTKSDALAGQVAGLVGRLPPVRERLKFDVTLMD
                     RVVGADSEQIIEPLKHKEPLMLAVGTAVTVGVITNTRKNQVEVQLKRAVCAETGARIA
                     ISRQVGGRWRLIGMGVLVE"
     gene            44178..44558
                     /locus_tag="PK196_00510"
     CDS             44178..44558
                     /locus_tag="PK196_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HON39938.1"
                     /translation="MKVLLDTNALLMPVQFGIDLYDGLMALLGDFEPVTLEEVVGELS
                     GLARGHGRDAAAARVGLEMARRATIVPSGSSAESVDGRVIEYARREGCIVVTNDRNLR
                     NDLIREGIGVVSMRKERTLELMRG"
     gene            44568..45137
                     /locus_tag="PK196_00515"
     CDS             44568..45137
                     /locus_tag="PK196_00515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HON39939.1"
                     /translation="MYYKVTLEDKVRVPPHRLGEDLERVILNVLQEQLEGSISKEIGI
                     FIAVTGILDVGEGELIPGDGAVYYDVRFEAIVLRLALQEVIEGEVVETTSFGAFVSLG
                     PIDAMLHVSQISDEYINYDEKNGRLICQDSKRSIAAGDGVRGRIVALSLNEREPRESK
                     IGLTMRQPGLGALAWLDEEFEEEKKRRSA"
     gene            45139..45342
                     /gene="spt4"
                     /locus_tag="PK196_00520"
     CDS             45139..45342
                     /gene="spt4"
                     /locus_tag="PK196_00520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HON39940.1"
                     /translation="MAVGGKKVVRVCRECHRVVEGEACVICGTSNLSEDWAGYVVIID
                     PEHSDIAKKMNITLPGRYALKVR"
     gene            45342..45827
                     /locus_tag="PK196_00525"
     CDS             45342..45827
                     /locus_tag="PK196_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HON39941.1"
                     /translation="MLRLPEAHRELFKKPFGVLYGSVAELLPLIEGRVVCAVGDVVTH
                     NLLAAGVVPDIAIIDGYTMRSPCNRSPLLRGRRVTVKNPPGTITDDLVAAIDEAVRNR
                     PVVIFVEGEEDLAVIPLVFAAPPGVAVLYGQPGEGVVLRIVDEAAKQEAAAMLERFIR
                     E"
     gene            45903..46196
                     /locus_tag="PK196_00530"
     CDS             45903..46196
                     /locus_tag="PK196_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HON39942.1"
                     /translation="MEFKITRDVRNEVLKRRELEFVLTFNGPTPSRKSIQEKLAALQN
                     RDEGLIVLDLERTRFGKMELLGRARIYDDEETKVSTEREYLLKRGKPKAESEA"
     gene            46202..46357
                     /locus_tag="PK196_00535"
     CDS             46202..46357
                     /locus_tag="PK196_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HON39943.1"
                     /translation="MASKRYECYEVKGNAAVLLKRHCPRCGPGVLMAEHEDRVACGRC
                     GYTEFRK"
     gene            46451..48034
                     /locus_tag="PK196_00540"
     CDS             46451..48034
                     /locus_tag="PK196_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HON39944.1"
                     /translation="MSDKRPLEGLVLGLEGTAWNLSAALFGEEPAALHSSPYVPSKGG
                     IHPREAAQHHASVMKDLISRVLTDPERIRAVAFSQGPGLGPSLRTVATAARALSIALG
                     VPLIGVNHCVAHIEIGRWATGFSDPIVLYASGANTQVLGYLNGRYRIFGETLDIGLGN
                     ALDKFARYHDLPHPGGPAIESLARSGGYIELPYTVKGMDLAFSGLVSAAQESTAPLED
                     VCYSLQETAFAMCVEVTERALAHAGKDEVILVGGVGANSRLQEMLRVMCEERGAKFAV
                     PERTYLGDNGAMIAYTGRIMLDHGVTLPLEESQIRPGYRADEVDVVWRTEPGEIFAGG
                     PHEGGVARGAEGVVEIGEDTVAKRRLSKRYRHPALDRRLITERTRAEARLISMARRAG
                     VPTPVIRDITTDTIVMERIRGDLLKYVAAPETIRVAGETVGRLHAAGIVHGDLTTSNM
                     IVRDGQCVLIDFGLASTSSEVESRGVDIHVFFQTLESTTANSGELIEAFIEGYSGVFP
                     DADEVIEREHEIELRGRYL"
     gene            48031..48591
                     /gene="rdgB"
                     /locus_tag="PK196_00545"
     CDS             48031..48591
                     /gene="rdgB"
                     /locus_tag="PK196_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HON39945.1"
                     /translation="MKITVVTSNANKAREVAAYFEGVLEVEHAALECPEFRHNDLGEI
                     ARGKAEFAYERLSRPLIVDDTGLFIDALGGFPGPYAAYVQDTIGNAGILKLMEGVEER
                     SARFETAIAFAREDGIRIFRGALPGAIVAPRGAEGFGYDPIFEYAGQTLAEMPLAEKN
                     RVSHRARALQAFRAWIEQETGERQNR"
     gene            48632..48778
                     /locus_tag="PK196_00550"
     CDS             48632..48778
                     /locus_tag="PK196_00550"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HON39946.1"
                     /translation="MARFPEAEARLLNVKICMKCNARNAVRATHCRKCGSDELRPKSR
                     ERKA"
     gene            complement(48842..49492)
                     /locus_tag="PK196_00555"
     CDS             complement(48842..49492)
                     /locus_tag="PK196_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HON39947.1"
                     /translation="MSGIDPEALADAVYGIFEIYLNRELRRRGPYLFELVEQGTDFKE
                     ELREVFSRFREDYPELADALLKRFGSIDAIYAPLREGEGVSPSKTTRTYWIVQDAPGP
                     HERGVDDERAGKWLIFVPAGEADEAWRKVRNETVEGRLGISAKVSTARPDPDSRDERT
                     VIYVYTKDWADEADVMRVRERLRELGFKERIGYKRNIETFRGEYSEEGRKVTYYSA"
     gene            complement(49534..50298)
                     /locus_tag="PK196_00560"
     CDS             complement(49534..50298)
                     /locus_tag="PK196_00560"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HON39948.1"
                     /translation="MRLNEVTISRAILEEQHRALIDLLEVDAAVIGGGPSGLTCAALL
                     GERGIRCALIEKKLSIGGGMWGGGMMFPRIVVQEEARRLLDRFGVACREFEEGYYVAK
                     SVETVAKLTAAACDAGVEFFNLVTVEDVMVRGDGRVGGLVINWTPVDMAGLHVDPLTI
                     ACTCTVDATGHDATIARMIERKGGNIQVKGESFMWAERAESRILEHTKEVYPGLFVAG
                     MAANAVAGECRMGPIFGGMLLSGERAAELVAEALDR"
     gene            50442..51323
                     /gene="htpX"
                     /locus_tag="PK196_00565"
     CDS             50442..51323
                     /gene="htpX"
                     /locus_tag="PK196_00565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HON39949.1"
                     /translation="MKWTRDFGLTMRMILTSLLLLIVYLIFLGILAALGFPFESLLLV
                     AALMAFLQYYFSDKLVLWSTRTRIVGEDEYPELHRMVESLAARAGLPKPKVGIMVSPV
                     PNAFATGRSPKNAVVAVTDSIMRTLNREELEAVLAHEISHVKNRDMLTLTMASFISML
                     AFLIMRNWIFISLFNDRDNNMGALILVYVVSIVVWLVSTLLTRALSRYREFAADRGSA
                     VLTGNPRALISALTKISGRMDYIPAEKKQEIEGANAFFIIPALSGNTLMELFSTHPPL
                     EKRVAALQELEAQMRGY"
BASE COUNT        10193 a        14973 c        15627 g        10410 t
ORIGIN      
        1 acgtgcgtat ggagttcctc aagcgttgag taaattctgc cgcagaccgg gcagataaac
       61 tcgcccatct ccaccgggaa atccgggggc ttcctgcccg gtggtctctc gtccattatc
      121 ggtcaccctc ttcggcttca atctcctcga tcgttcgccc tgagattacc gatcgaggtt
      181 ctgaaacgac tggattcctg ccggggtctg cctcatcgtc gttcttcttc accagaagcc
      241 aggcctcgtt ctttcccgtc ctgaaacggg tgagagcgta gccgccccgg atctttgttc
      301 catcgagcca gaaggatatg tgaccctgcc tgagtccctc cgcaacccct gttttctcac
      361 ctcttctctc tgtaaggttg cggtaggttc ccctgtccca gacgagaacc gcaccggcgc
      421 cgtaactccc ctccggtata accccctcga actcggcgta ttcaatggga tggtcctccg
      481 tcgggacggc gaggcgtttc tcctccggag aaagggacgg ccctttcgga acagtccatg
      541 acttaagcac gccatcaacc tccagcctga agtcgtagtg gagcgttttc gccgcgtgtt
      601 tctggattac aaagattgga tggtcctgct ccaccctctc ccggtcatcc acctgcgtat
      661 ccaatcctgg catacggaag agtgccgtgc tgcctcaaga taaggttttt cctggttcag
      721 gtcttccgct ctgatgtggg gacgaccctg atgttcatct tctccgagcc gatgatcagt
      781 ataccctccc cgggctggaa gttgaggaga tccttctcgc tcacctcgaa gaacctggcg
      841 ttctcctggg cctgcttctt gttctctgag cgcatacaga acttcacggc gatgtttgcc
      901 aggatctcct cgttaaagtg agcgatgagc tgtgacgcca ggatcagcga gaccccgaac
      961 ttccgcatct cggtggcgta cttgttcagc accgccttga tggccgtctt cgacccgctc
     1021 ttctggctca ccaggagttt tgcctcgtcg actatgacga agagccggaa cttcgctcgt
     1081 tcgtcatcgg ttccgcgtgg gatctccccc atagcctgaa gggtataata gatccggcgg
     1141 aggaatagat cggcaaagag gtagcgcagg ctctccggga gggcgtttaa gttgatatgg
     1201 gtgatcccgc cggagagtat ggcggggatc gagatcttct cctctcccga gaagaggttg
     1261 tagtcaaaga tatcctcgag gtatgcaggg atcgtctcgt cctcgcagga catgatcccc
     1321 ccctcgatat catcaaagtc gagcacccgt gtccatgttg cggtatccgc cgtgatcccg
     1381 gcggtcctgt agcagtcctt tatgatgttc tttaactttc ggcgctgctg gatgccgagt
     1441 gtcgggaagt tgatcgagat ggcatcgaca aagtccgatg tggcccggag aggtgtgatc
     1501 tcatctatcc cggggtcgag ttccagcggg ttgaagtagt actgaccccc ctcccggatc
     1561 ttgtatgacc ggatatcgct gcccgtccct gccatgtccc cgtgaaaatc gatcaggaga
     1621 accggcatcg attgtgccgc aagttccgag gcgatacacc ggattgtctc ggtcttaccg
     1681 gctcctgacc cgccaacgat gatcatgtgg ccgttattga gcctccctgg cgaccagttg
     1741 acccgctgcc catcaccggt gtagccgagg aagagatcaa gcccgccgga gcgctggggc
     1801 tccgtcacag gagcgtttct cagcggtgct ggcgtggggt gtgccacagc cgcggcccta
     1861 tcttcaatcc gctccccctc ctccggggga attgcaagat catcccacac ctcctcaccc
     1921 acctctctgt ggccggctgc cggtgactgg atggctgcgt cttcactgaa agggtgcgca
     1981 tcctcgatct tcccgggagg ttctgccgcc ggcatgggtt ggaggtcgat aacgatcttc
     2041 ccggcaactt cggtcataag tgcatgctcg aggtcgcgaa ggcgcttctc gtagaggttc
     2101 cggtccctga cgatgaacct tgcaaggtca accccatcat cctctggcac cccttccagg
     2161 acgtagacca cgtcggcaag gctcgcaagg acgcgtgaga gttcgcggcg gagggcacgc
     2221 gatccgcgga tacgctcgta gtcctcgccg aaagcaaggt atcgcagaac tgtgaacgtc
     2281 gagtagaacg actcaagaag catatcgtag tgatccctta ctccagcgtc gatccgttcg
     2341 agcatctcct tgaggatata ggcaaactcc ggcgggaagg tgtagacaac accgtcgatc
     2401 atgccggtgt caaggtcgca tgagtaggcg aggacggcgc accctgcgtc tttgagcctg
     2461 gcggcgaacc gttcgcaccc ctcaaacagg cccgggcagt ttttgaggag gaaatttatg
     2521 aacgtctcat cgctgcgggg cggcgttgat gggaacgtct tgtttatgaa agaccctttc
     2581 accgtacctg ccaggagaac cgggacgatc tcgtgtgtct cacgggctag accctggata
     2641 tccagtctcc ggagcctcgc tttgagcagg tcccgcccga tcgcctcgcc cgtggcggtc
     2701 ggccggacca gtgtcgtccc gtgccaggag agtgactcga tgaggccaat gtctgtaagg
     2761 gcaaatagcg gccctgaggt ataggcgtag caactctgct cgatcacgcc ctctccttcc
     2821 ccgcgggaag aggcgctatt ctgggctaca tagatgtaga ggagtgtctc tatgatgtcg
     2881 cggacctcct ggaaccggaa atcccgcagg tactcgatga cggtggaagg aaacgattcc
     2941 atcaggccgg agatctcggc atctgaatag cccatcgtcc ttagtagttc ggctatctct
     3001 gttgacacgg gttcaccagt gtattcttat ccaggtcggc acgagaggtg cacggtgcca
     3061 cttcacgcga ttgcctgtct ccggtgagga aggcggtatg aattggtttg ctctctaacc
     3121 taataaacat aggttttttg cagaaatacg gctgcatccg gcaaatcctc ccgcgccggg
     3181 gcccgggtgc tggatgggtg gcaggtgcct gcctgcgtga tcaccgctgc cggatnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcccg ggtgctggat gggtggcagg
     3361 tgcctgcctg cgtgatcacc gctgccggat ctcatgcggg cggatgcccg gtgacggtga
     3421 gactccgccg gccgttctca ctgccagacc ctgaccgggt tgatcgaggc atcgatgagg
     3481 aggtcaaagt caaacatctc gacccagccc atcttctcaa acatctgcat gaagcagaca
     3541 ccgacgatca ttgctcctgg atgggtggcg acggccccgc gcaccgcttc ttttgtgacc
     3601 gggacgcccg gcatcgagag tccacccatc aggactatca ccttcgggtc gaccttcacg
     3661 gccgattcgg cggagacctg cataccgacg ccctcgaccg gccggatgac ctttgccgct
     3721 gcctcgttga cgtagggcac gtaaacctgt tcgataggga ggtcgcggac ggcaaacccc
     3781 aggagttcga tgaagggtgt gcaggtgccg gggcagccgt agtagacaac ctggtcgccc
     3841 tcatttagcc cagaatcgcg aaggaatcct ttgaacggcc ggagcattcc cgggacgcct
     3901 gagagttgtt ctttcagttc catgcaggtc ttgttatcgc ctggagatat accatttccg
     3961 gatgtgaccg tacgttgcct acagcactcc gcagcctgag aggtggtaga gtctgcggac
     4021 acgttcggcc gccttttccg ataccctctc cccgatatgg gcaaggatgg tctcgatgtc
     4081 ctcttccggc acgcatccca ggtcttcccg gccggagaga atcaggtccg taagatacgc
     4141 ccagtcctct tctgagagtc gggcaacccg ttctgcaaaa tccttatcat ccctggcaat
     4201 gtggtttgag accggcggaa ggatggagtg aaactcatgc ccggacccgg ggcagaggat
     4261 accactgtac tcaggggcca gttttcgaag gatggataga tccttctcgt caagttcgcg
     4321 agccatggtt taccggatat aatggagttg tgttcgttca aaaagtgttt tgtcccctgg
     4381 cccggttacg acgctgtgag ggctttttgc tcaggagtca ggcctggccg gggtggtaaa
     4441 cgagagaagg aatgcgtggt ttcgactcat agagaccagg ggtgatctca gggccaaact
     4501 gccgccagat ggttcgttgc gctgctgcgc cgcgaccact ccgcgtgata aaatgcggaa
     4561 gaaaagggaa tggccaacaa cccggccccc caccttactc tcctatctgt gcctggatgt
     4621 gccgcaccgt ttcggggtaa tgtcctgcaa ggtggttatc cttgaggttc tccctcacaa
     4681 tccaccggat ctcctggtca tccagtgttg agacctgctg caggtactca aacccgctgg
     4741 acgggagggc ggcaacgacc cggctgagcg tgcagccaag cgtctttcga agcgcctgga
     4801 aatcttcgga ctctctctca ccagcggtgt agacgcggat catgatcttc cgatggagcc
     4861 ggagcgccgc ctctgcgaga tctggctctc tgagcacgtc cgcgtcagca accccggcgg
     4921 caacggtctg catggcggac catgaacccc cctccaccca gccattcagt tctgagacgg
     4981 tcacgtcccg gtgccgggcg aggagatccc tgagcccgat cgccactgca tcccggatcc
     5041 gccacctcgg gtcttcggcg ctctctgcaa gtttccggag cgccgccttc acgcatccgg
     5101 gtgatatcgt gcccatcgcc gcgatacccc tgactccgca gaccgagagg aactcgtgcg
     5161 ggtcgtccgt cggtgcatct tcgggtgata tgcaggcgag ttcgacgcag aggtcccata
     5221 ggatccggcg gtcttcccgg tcagcgacag caaattcccg gattgccctg gcaaacgcct
     5281 ccgtcagatc ctggtttgtg cgtgggcccg cgaggctgct ctccgccgcg aggtacgcgg
     5341 cgaggtctcc ggcgtctccg cttgagagga gatccatgag gcgcctggca acttcgcggg
     5401 tataaatgga gtcaggggtc aatcgtgtgt agagaggtgg caacccgcgg gcataaaaag
     5461 atcgctacct ccgggagagg gtttcaacaa aggtctcaag tgccgcatgg acatcctcgc
     5521 tgtttaaggc agagatgggg aagaccatct ccccgtggag atcgcggcag atggcgtcgg
     5581 gcatcgcatc aggacagtcg cacttgtttg ccataaacgc gagtggcacc tcaagcgttt
     5641 tcagccgggc atacagatct ctggtcatgg catccacgcc ctttgttgca tccaccatga
     5701 tgatagcgcc gtccatccca cgcgagagga cctgacggac aaactcgaac cgctcctgcc
     5761 cgggcgtccc gaagaggtag acagccctcc cgttgacttt gagtctgccg aaatcgagcg
     5821 ccacggtggt agggccgtcg tctgaggagg cctcgatgtg acggctgtgg gggtcaagcg
     5881 cctgaatgaa acttgatttg cctgcattga atgatccaaa cacgacaatc ttgagtctgt
     5941 cttctgccat caggcttcta ttagcgagaa gatgttttag ggtttcgata tgattgcagc
     6001 atggacgaga cagcaatggt tccgggctgt catgggaatc atattggtac tctcccgcgt
     6061 ctcatggaaa gattgttccg cagtcgatgg cgcaagcggc actgcccggt tacgggggcg
     6121 caggtcaccg aacaaccata tactggtaaa atccaaccta caggtatggg gccagacgag
     6181 atggatcctg tagtgctgga gatcatggca gttctggata gcctcttcct cgccgagagg
     6241 caggcaaggt tccaggtatc cgccctcgag gtgcaggact acccattcgc agcgacattc
     6301 gagatggtca gggacacgga gtctgatgct gccatcgagg aggcgctcag cggtttcggc
     6361 tttgaacagc acaccctgga tgacgatgct gaactatgga tctcggatga actcgggttg
     6421 atggtcttcc tcttcttcac cacgccggat ggccgctcct acacctacag gatcgtcagg
     6481 ttcgaggtgg ccggcgagga tgaaatatct ctttaaaaat gtggatactg gggaaaaccc
     6541 acgtattgct gttttgtggg ctgccatgcc agatgctata tactgtaaga tacacttctg
     6601 tgtaacgggc aaaagacagg aggggcatat atggagcgct cggtaaaacg tctcatggag
     6661 gacagggata aacgtcgcga ggagaacatc gagaagtaca ttgagcagct ctctgacgag
     6721 agcaccccct accggctgcg ggcggttgaa gcgctcggga cgtgcagcga tccgcgggtg
     6781 gtcgaaccgc tgattgccgc cctggatgat cccgagaacg aggttcggtg ggtcgcggcg
     6841 caggctctcg ggaaagtccg cgacccgcgg gcggtcgaac cgctcctatc tctcctcgct
     6901 gaccatgacc gctgggtacg gcgcggtgct gcctggtcgc ttggagagat cggtgacccc
     6961 cgggcagtcg aaccgatcct tccgctgctt gcggataaga agaaggatgt caggactgcc
     7021 gccgcagacg cgctcgggaa acttcgcgac tcgcgggcgg ccgcggccct ctccgccgcc
     7081 ctcgaggacg aggtggagcc tgaggtcagg acggcgatcc gccgcgccct tcgcgagatc
     7141 acaggcgaag cggagccctg aatgggtgaa gggggtgctt tcggtgccat ccagcgtgcc
     7201 ggaagccctg atccccggct atatacgtaa cggtcattac ggacgtccga accccctggc
     7261 aggacgatct ttgccgtaat ttctgtgcaa tagcataggg gaagcataag ggaagcgccg
     7321 tcactcgaaa tctgcacggt aatccccgga tccacgaccc tcaggagcgg ttccggggtg
     7381 aacgagacca ctttctcgat ggacttcatc atataacccc cagcctgttt ttttgcaccc
     7441 gctcgtgacc taccctcacc ccaggaatct ccgtcggttt gcagcctcca ggatgagcgg
     7501 ggtcaaccca ccggtctcgt tctcgcgcag cgcccgcgag atggcggtcg tgaggcgggc
     7561 atgctcctcg tcctggacga cgtccaccag gtcagtcacc ctggctgcaa ggtccggtgg
     7621 aacgaaaccg gtctctgtgt tgaggaaccg gcaggcggct ctaagatcct catcaaagtt
     7681 gacatagact ttccggataa actcgatatc cgcatcatcc agcacattca ggcccagtat
     7741 ttctggagga aggccgatcg agtagaaagc ggcggtgaac ttgattgccc gtggcagggt
     7801 gatcccgcct gtgtttcgcg catacccgaa gagaccgata tggagtttcc gtttccgtct
     7861 ctccggaaca tatcgggcaa cccgatttat cagaggggca aacggtccaa gccagcgctc
     7921 atatgcccgc gtatacctcc agatgatatc aagtgccgca tcttcttcga tcttcgtggg
     7981 ttttgcgggc tggcgttctt ccagcacccg gatagcgttc tggacatctt caagctggta
     8041 gtcatacttg aacgccgact gaacggtaaa cgtccggacg ctgggatact cggcagcgac
     8101 ccgatcgacc gtcagcggcg tgagacctcc tctaaaaggc gccgaaccga cacctatgat
     8161 cggatgaatt cctgctccgg cctcttctga gaccccctgg agattctgga gagcgatctt
     8221 gttgaggagg acggcgctga tgagcccgta gttcattgcc gggtctgacc gggcaaggaa
     8281 gacacgctgg tcagcgatat ccttgtcctg gaggtagcgc ctgaccacgt tgtgggcatc
     8341 aagcatcctg gccatatcct caaagagagg tatgacgttg attcgatccg gtcggaatct
     8401 tccgatccag tcagcgatgg ttggcccacc ctcccggagg cgtctctcct gttttccgac
     8461 gacaacgtct ctatagtagc ggtagatgtt gtcgatggcg agatgggagg tggtcatcgg
     8521 caggataacc tcaaatatcg gggagcattc ttccccgtaa aagaggcatg ccgtatcaaa
     8581 tgaccgcggg atgctctcga gtgtttcaag cagtatcttt gcctcggcgc tctcgacctc
     8641 cggatttgga acgcgcattg tgaggaaaac atcccgtcca agccgtttct cccggaagaa
     8701 agtctcgtag cggctcagga gtttccggac cacaaagttg tcaacctctt ttccctcgca
     8761 gtcccacatc tgttcgctgc agccgaggtg ggagtaagcg tagtacgcct cctgcacctc
     8821 atcgtccccg ccgagttcgg gactctcggc aaaaaaaggc aggttaacgt tgtcagggtg
     8881 ctgtgtgctc atgcactgcg ggatccgtat catcttcttg ctccacgtag cgggtcgtgt
     8941 tctcgtgtcg accatatcat tctgcaatga ttgagggaaa aactctccgc ctgctattta
     9001 tcctgcggcg gtgaagatag cggagcgaaa agatcccggc ctttatggcg cggtagttcg
     9061 cagcagtgtg ccctaaccct tcacctcatg gtctcgtacc gcattctgcg gcctgcccgc
     9121 cgcaagttaa agatggtaag agagcttttc tggcacagaa ccgctggaat tatcagccgc
     9181 atccgctcac cgtggatgat aaaaagaaga tgtgagggta tcggggttga ttacgacagg
     9241 tagtcttcag gccgcgggat ctgctccttc aggcccttgc ggcggcggat gctcctgacc
     9301 acctcttcca ccaggcctgc cggaaccagt tcgaacccgg cgaactcggt gctccacatc
     9361 gcacgccctt ctgttgccga cctgatatcc ccggcaaacc cgaagagttc ggcgaccggt
     9421 gccttgccga tgaccgtcac cgtgtcgcct tcgctctgca tatcaaagat ctggccgcgg
     9481 cggccctgga tctgcgaggt tgccgcaccc atcagatcgg ccgggacggt gatctggatc
     9541 ttctggatgg gttcgaggag cgaatcgccg gccatcagca tgcctgcctt gacagcgctc
     9601 ctgaccgccg ggatgacctg ggcggggccg cggtggatgg cgtcttcgtg gagtttcaca
     9661 tctaccagcc tgaccttcag gttctggacc tgctcatcgg cgagcgggcc accgcggagc
     9721 gcttcgcgcc atccttccag gatcaactcc atggtctcgt tgaggtactg gatacccttt
     9781 gtcatgtcga tgaacatgtt ggtgccctcg atcgccctga cgttcttggc ctcctcttta
     9841 tccatccctg cggcgaccag gatgtcacgc cgctcaagcg cctgctggtt catcgagacc
     9901 tcgccatcat ggatcaactt cacgatcgcc gggtccatcg gttcgagctc gatgtagaac
     9961 cggttgtggc ggttcggcga cttcccctca accgggcccg cacgacccgt gatcgtctcg
    10021 cggtatacaa cgatcggcgg agatgtgatg atatccacac ccttgtcgcg cttgatacgc
    10081 ccggtgatga tctccaggtg aagctccccc atgccgctta tcaggtgctc cccggtctct
    10141 tcgttgatcg agacctgcac agtcggatct tccttggcca cctgacggag aacctcaacc
    10201 agtttcggga ggtccttcat gttctttgcc tcgacagcga cggtcatgac aggttcggag
    10261 tagtgtttca gcgactcgaa cggggtcata tcaatgagtg tcgtcacggt cgaaccaacg
    10321 atggcgtctt ttaagcccgt cacggcggcg atgttgcctg ccgggagcag atcaacctca
    10381 accctctcgg cgcccataaa gatgccgacc tgggcgatac ggtttacccg cttcgccgtc
    10441 cccatgatgt agcactctgt gcccctgcgg agcgttcctg cgaagaggcg gccggtggcg
    10501 acctctccgg catgagggtc aaacgagata tctgtgacca taaggcatac ggggccgtta
    10561 gggtcacagt tgatcatagc cttcccttcg ggggtgctat aatcaccgtg ccagatgatg
    10621 gggatacgtc ggtgctgggc ctcgattggg ttcgggaggt gcttgaccac catgtcgaga
    10681 aggactgagt gcagggggct gttcttggcc agccacttca tatcgccgct gttgcacttc
    10741 tcatagacct ctttgaatga gaccccgctc tttttcatga atggtatgga gacggcccac
    10801 ttatagaggg cggacccgaa ggctacggtg ccctttgcgg catccagttt ccagccttcg
    10861 ttgtacatct tctcgttcat acccttgatc agcttgttga ccttgtcgat cacccttgca
    10921 aggcggatct gcatctcctg ctcgttcacc ttcagctcgt tgatcagccg gtcgaccttg
    10981 ttgatgaaga gaaccgggcg gacaccttct ttcagcgcct gacgaagcac agtctccgtc
    11041 tgtggcatgg tgccctctac cgcgtccacg acgactacag caccatccac cgcacgcatg
    11101 gcgcgagtca cgtcaccccc gaagtccacg tggccagggg tatcgatcat gttgatcagg
    11161 tactccttcc cgtcgtattc gtggaccatc gagacgttgc ttgcgtcgat ggttatgccg
    11221 cgcacctgtt cttcctcatc ggagtccagg aagagctgcc ggccggcaag ctcctcgctg
    11281 atcatgcccg cgccggcaag caggttgtct gagagggttg tctttccgtg atcaatgtgg
    11341 gcgacgatgc cgatgttccg gatctgctcc ggtttgtcca tcagttccgt cacccgctct
    11401 accatcttct ttcgtcgggt catgaaaaaa caccaaaaat aatggagggt ctttagcggg
    11461 ctgcctttgc aatacgttcg cgctcttctt tcttcgatat ggcatacgac cgggtgtcac
    11521 cgttcgcagc tgcgataagc tcctcggcaa gcgcctcgct cacgctcttc ttcttcttgt
    11581 ggcttgcctg atatacgcca tctgttatga accgcagcgc tgtatcgacg cggcgctggg
    11641 gtgcggtgtc gaccgatttc ggaacgttga tgccgccgta cttcagcctg acggtctcct
    11701 cacgggggcc ggcattcgcg acagcgtcaa ccagaacctg taccgggttc tttctggtct
    11761 tctggtagac gatctcgaag gcatcacgga cgatgcggat ggcaagttcc ttcttgccgg
    11821 tgttgtgttc agtctgcatc agcttgttga tcaggcgctc cacgatcagc atgttgctct
    11881 tgttgaactg ctggccggcg agcttgccgc atgtatgcgg cacgatcatt gtgtggaggt
    11941 tcacgtagcg ggcaaggctt ggatcccgga tctcaacctc gctcatgtcc cagcggttga
    12001 ataataactg ctggacgttt gcttccgtct ctgcttccgt cattgtcgct tacctccgcg
    12061 gcttctcctt gcgcccgatg accatctcac gcaggctgac gttgttaacc ttggtcacaa
    12121 cgtagcggac accggggata tcacccatcg acctccccag tctgccgccg atgccttcga
    12181 tctcgacctc atcatgctca tcgatgaagt ttatggcacc atcaccgacg gcaaacgccg
    12241 ttacctggcg tccgtttttg atcaactgca cgcggacgca cttccggatg gccgagttcg
    12301 gctgcttcgc ctcgacaccc accttttcga gaacgatccc gcgggcctgc ggcgcccctt
    12361 ccaacgggtc ggacttcaca tcaagtccga gctggcgccg cgcgtagaca gggtcgtgcc
    12421 atcgcatctt ctttgaatcg cgcttcagtt ttcgggctgc aaatttaccg tttcccatcg
    12481 aataccctcg tatctgttaa atttgtagat aggagcaact cctgctgtgc tataaaacaa
    12541 cgcgcgttac ggatttatat attgtggaaa ccctcctctg cgccggttgc ttatccaggt
    12601 aatttgatct cgacctgata taatattata tcccggcggg caaccttcat tgatgagagc
    12661 ccggatatat aaagaggtct ttttcgaggc gacccaccgc ctgatccact accggggaaa
    12721 gtgttttcga ttgcatggtc accagtggcg ggttgaggta tggatcgagg gaacggccga
    12781 tgagcgcacg ggaatagtcc ttgactacaa ctgcatcaaa gaggtcgtgg gaaggttcga
    12841 tcaccaggtg atcctgaacg aggacgatcc catggcggcg tgcatcgaga cgtttcatcc
    12901 ggtcgtcacc acccgcggtg acccgacaag cgagaccctt gccggtgtga tcgcggatat
    12961 gatcgatgcc gaggctgcac ggcaggggag tgacgcccgt gttgcaaaga tccgggtctg
    13021 ggagtccaca tcctgttacg cagagcgcac ctatgatcgt cagtgagatc ttccggagcc
    13081 ttcaggggga ggggaaaaac caggggcggc cctgcacctt tgtcaggctg gcaggctgca
    13141 acctccggtg cgcctggtgc gacacctcct acgcctggaa cgagggggag gagatgagcg
    13201 ttggggaggt tctcgaccgg gtctggctgc tccgcggcgc atacatctgc atcaccggcg
    13261 gggaaccgct cctgcagcgg gaagatctcc tggaacttct gaaaaagttc aaacttcatg
    13321 gttattcagt cgagatcgag acgaacggca gtctggattt ccgggagatg cagccttacg
    13381 cctccatatg catggacgtt aaatgccctt catctggcga gagaagcgat ctgtcacttc
    13441 tttcggcgat aaccgcgcat gacagcgtca agttcgtggt ggctgatatg gacgacctcc
    13501 tctacgcgag ggctgttatg ggccgctgcc ggatcggggg cgaggtcttc gtctcgccgg
    13561 tggaggggtc tgattgccgc gccattgcag actatatagt gagtgagaat cttcctgtga
    13621 ggtttcaggt gcaacttcac aaggtcctgg ggatgagata gatgaaagca gtctgcctcc
    13681 tctccggcgg catggactcg agcacgcttg cctacgttgc aaaggatatg ggttacgata
    13741 taatcgcgct ccatttcaca tacggccagc gaacggagga gaaggagcgg gagtgtgccc
    13801 ttttgatagc gcgtaagctt gaggccatcg agtttgtcga gattggcctt gagcacttca
    13861 ggaagatcgg cgcctcgagt ctgacggatc catcgatccc gatcgaggat tacgctggtg
    13921 agaagggagg tgtacctacc acctatgtcc cgttcaggaa tgcaaacctc ctcgccattg
    13981 caaccagtct cgcggaagcc cggcgggcgg aggcgatctt catcggcgtc cagacggggg
    14041 attacccggg gtatcccgat tgccggccgg agttcatcga ggcgttccag cgggtgatcg
    14101 acctcggaac ggcggctgac ccgcggatca ccctgatgac accttttgtc cggatgacga
    14161 aggcggatat cgtcagggtg ggtcttgctc tcggcgtccc ctacgaactt acctggtcgt
    14221 gctaccgcag cagcggccgg gcttgcgggg tatgctcatc ctgccactac cggagagagg
    14281 ccttccgtga actcgggctt gaggatccaa tcgagtatga ggcatgaaca tggagatcta
    14341 cagtggaaaa cgtctcaccg ttgagaaaaa agtggtcacg ctccctgacg gcgagaagaa
    14401 ggagcgggtg atagtccatc ccgggggggc ggttgcgatc ctccctgtag agggggatgt
    14461 ctgccacctg atccggcagt accggtttgc gattgacgac tacatactgg aagccccggc
    14521 aggcacgatc gatgagggcg agaagccaga ggagaccgcc taccgcgaac tgatcgagga
    14581 gaccggcatg aaggcggaga ggtttatccc taaaggctgg atctacccat ccccaggtta
    14641 tactgacgag cggatctggc tgttcttggc ggagggtcta tccccgagtt ctgagtatag
    14701 gagggatgat gatgagttta tcgaggttct ccgcgtcccg ctttccgccc ttcccggaat
    14761 gatcgctaac ggaacgatca tcgatgcaaa gacgatctgc cttgtctgcc ggtgtcttgg
    14821 ttgagtgttg cagcgaatgg gcgcaagttt atgcgatccg gggataaaca gatagtagct
    14881 tcgatgaatg aacatcaata ataaagcttg gtgaaccccg atggtagaga cgcaagagat
    14941 ggaaaccgcg cgaaagcgct acgaatttaa aaagacgctt gagaggcttg cggagaagga
    15001 ggggagcggc accgagctta tcacactata cataccgccg gataagcaga tatccgatgt
    15061 gaccgcccag ctccgtgacg agttcgggca gtgctcaaac atcaagagca agcagacccg
    15121 gacaaacgtc cagagtgcaa tatcctccat cctctcccgc ctgaagtact ataagcgccc
    15181 cccggagaac ggcctggcga tcttctgcgg cacgataaac gttggtggcg accgcacaga
    15241 tctccagtgc gagataatcg aaccgcccga accgctcaac acctacatgt accgctgcag
    15301 ttccacgttc gaactcgaac ccctgcagca gatgctggag gagaaggagg tctacggcct
    15361 tatagtcatc gaccggcgag aggcttacat tggtttcctg cggggcaacc ggatcgaacc
    15421 cgtccgcggc gtcacctcga ccgtgcccgg caaacagaga aaaggaggtc agtcgagcgt
    15481 ccggtttcag cggctgcggt tgatcgccat caacgagttc tacaagaagg ttggcgatct
    15541 cgcaagtgag atatttcttg gagagaaaga tttcttcgag aggttcaagg gtgtcctgat
    15601 cggcggcccg accccgacaa aggaggagtt tgaagccggc ggctacctgc atcacgaggt
    15661 gcagaaacgg atcatcgggc tgtttgacgt ctcctacaca aacgagtcag ggcttgcaga
    15721 actcgtcgag aacgcagcgg atgcacttaa agggcttgaa gtcatcaagg agaagacggt
    15781 gatgaaccgg ttcctccagg aactggtaaa ggatgacgga gcggccgctt acggtgagga
    15841 gagcgtgcgt aagaacctgg agcttggtgc cgtcgatacc ctcctcctct ccgataaact
    15901 tcgtagagca agactgaaac ttgcctgccc gaactgtgat tacaccgagg agcgaacggt
    15961 cagcgtcgag cctggcaaac atatcaagga tctcgacctc ggcacctgcc caaacgacac
    16021 ctcgccgctc tatgtggccg aggagagcga catcatcgat gaactgaccg cgcttgctga
    16081 ccagagcaac acgacggtca agatcatctc ggatgatttt gaggagggct cgatgctcta
    16141 caatgcgttt ggcggaatcg ctgcaattct gcgttacagg accggctact gatgtatcag
    16201 gagaaatacc accatattga acgcatgctc agggcatgca ccggtgagga ggacgtcctc
    16261 ctcaccgccg gaggggatca cgccgatctt gcctccacgg tagcgtttaa gcttgcaaag
    16321 aaggagaagc ggccgccgca ggagatcgcc gctgatatcg caaagaaact ggggaaaaac
    16381 ccggaactcg gcgatatcca ggttgaggcg gccgggccct atctcaactt ccgcttcggg
    16441 ccgacactcc ttgcggaggc ggtcagggag gcggtcgaac ccggttacgg aagccgtccc
    16501 cgccgttcca gccgggtcgt gctcgagcat acgagcgcga acccgaacgg cccgcttcac
    16561 gtcggccata tccgcaacac tgtcatcggg gacagtctgg cccgggcgtt ccggaaggct
    16621 ggctacccgc ttgaggtcca gtactactta aatgatatgg gccgccagat cgccatcgtc
    16681 tcctggggtt tcgatcacct cgacatcccc cgcgggaagg gcgagaagga ggaccattac
    16741 gttgcccgtg tctatatagc ggcgaaccgc gagatcgaga agaatccgga gatcacccac
    16801 gagatcgacc ttctcatgca gaaggtggag cgcggcgacc ccgtggccgt caagaagttc
    16861 cggagtgcgg tctctctctg tcttgagggg atcagggcga ccctcgccgc cttaaacgcc
    16921 tatcatgacc ggtttgtctg ggagagcgat tttgtccgga tcggtgacgt ggacagggtc
    16981 atcgaacgtg tccggcggct gccgcaggcc gtggaagatg agaccctctg ggttgaccta
    17041 tctgagtttg gttttgagaa gaggtacatc ctccggcgca gcgacggcac atcggtctac
    17101 agcacccggg atctcgcata ccacatatgg aaagggcgta actatgatcg gttgatcgac
    17161 gtgctcgggg cggatcataa actgatcggt gcccagctcc aggccaccct gaaactcctg
    17221 ggtgagaaac cccctgagat cgtcttcttt gagtttgtct cactccctga aggctcgatg
    17281 agcaccaggg caggcaagtt cgtctcggcg gacgaactga tcgaggaggt tgccgcgaag
    17341 gcgttcgagg aggtgaccgc ccgccggccg gaactccccg aaggggagcg gtgcgcgatc
    17401 gcaaaatcgg tcgcaatcgc tgccgtccgc tacgatatcg ttaaggtatc gcccgagaag
    17461 agcacggtct ttgactggaa ggaggcgctt gacttcgagc ggcagagcgg cccatacatc
    17521 cagtatgccc atgcccgtgc atgcagcata ctggagaagg ccggggggtt cgagccggcg
    17581 tatcactacg agaccgacca cgagatcgcg cttgccagac acctggccag gttcccggcc
    17641 gttattgagc aggttgtggc ggagctccgg ccgcacctcc tcgctgctta cgctcgcgac
    17701 ctcgctgacc tcttcaacgc cttctaccac tatgacccgg ttctgaagag cgagggtgag
    17761 acccgcaaca gccgtcttac actcgtggat gcggtcagga acaccttgaa ggaatcactt
    17821 gagaccctcg ggatcgatgc actcagaagc atgtaacgtc ctgcgcagac agggctacca
    17881 gttcatcacc ccggcctcct cggcagccgt gaaaccatgt atgtggtgca agcgggcgct
    17941 aaaaggcggg gagatgtgct acaaggcgca gttctatggc attgagagcc atcgctgtgt
    18001 ccagatgact cctaccctgc ggtgcaacca gcgatgcctc ttctgctggc ggtcgtttga
    18061 gcacgaggtc gtggaggagg aagagtgtcc gccggaggtc atcatcacaa acctccggcg
    18121 cgcccagaag aaggcgctcg cgggatacaa ggtatcgcct tacgtcacgc cggaacggtt
    18181 cgccgaggcc ctgaatccca cgatggtcgc gatatccctc tcaggggagc cgacctgcta
    18241 ctcacgcctc ccggaactgg tggacggcct caacgccgaa ggctacacaa ccttcctggt
    18301 ctcaaacggc accaggccgg aggttctcgg ccggtgccgg ccctaccaga cctacgtctc
    18361 cctggacgct cccgaccggg agacctacct gcgcctctgt cgaccaaagg aggattactg
    18421 ggacctggtg ctggagagcc ttgcagggct tgccgggcgg cggtcggcca tccgcaccac
    18481 cctggttcgc ggttacaatg acttcgcgcc cgaacgttac gcggcgatct accagggttc
    18541 cggcgccagg tttgtggagg taaagggtta tatgtatctt gggtacagta gaaaccgatt
    18601 gctgagggag cagatgccgg aacacgctga gatccgggaa ttttcagaga tggtttcgca
    18661 gcactcggac tatttcatta aagatgagag cccgctttcc cgcgttgtct gcctggagag
    18721 agatgtatga gatttgatgt ggaagagttc aaaaagaggg ccagagagga cttcgagcac
    18781 gcctggtatg aggggccgtc tgtcctgacg ccgccggggg tctcgggccg ctatccccgg
    18841 cttcgctacg cccgggcgag ggagcacccg atcttcgata ttgtagagcg gcttcgcgag
    18901 acatacctcg cgatggggtt tgacgaggcg atgaaccccc tgatagtcga agagtctgat
    18961 atataccggc agttcggtcc tgaagcaatg gcggtccttg accgggtctt ctacctcggc
    19021 ggtctgccgc ggccgaacgt cgggatcgca agaaagcaga tcgaggagat cgaggctatc
    19081 cttggccgtg cgctcactgc cggaaccgag gagaggctcc gtgagaccct ccacggctat
    19141 aagaaaggaa agatcgacgg tgacgacctg gtgcacgaac ttgcggccgt gctcgaggcc
    19201 gatgatgctg ccgtggtgca tatccttgat gcggtctttc cggagttccg cgaccttgct
    19261 ccggagtcgt cgcgcaacac gctgcgcagc cacatgacaa gcggctggtt cctgacgctc
    19321 tcatctatct gggacaggca gcggctgcca ctcaggcttt tctcggtgga tcggtgtttc
    19381 cggagggagc aggaggaggg gccaacccgc ctgatggcct atcactcggc ctcgtgtgtc
    19441 gttgccgggg aggacgtcac ccttgaggag gggaaagccg tcagcgaggc gcttctctcg
    19501 gcgttcgggt tcactgaatt caggtttcag cccgacgaga agcggtcgaa gtactacatg
    19561 cctggaacgc agacggaggt ctatgcacgc catccctcgc tcgggtgggt ggaggtcgct
    19621 acgtttggcc tctactcccc gtcggcgctt gcggagtacg ggatcggagt gccggtgatg
    19681 aacctgggtc ttggggtgga gcgtcttgcg atgatcgttt accagtcaaa cgatatccgg
    19741 cagctgacct acccgcagtt cttcccgcag gaggtctccg accgcgagat cgccggtgca
    19801 atccatctcc gtgaagaacc ggcgtctgtt gcggggcgac gtatggcaga gggtatccgg
    19861 gccaccgctg cggagcacgc cgccgccccc gggccgtgtg cgtttgttgc ctggagtggc
    19921 gagatcgcgg ggcgtgaggt tgaagtggtg gtcgaggaga ccgagtccaa cacgaaactc
    19981 tgcggccccg cctgcttaaa cgaggtcttt gtccataacg gttcggtgct cggcgtaccc
    20041 gataccgcga agtgggcaac cgtccgccag gagggcgtct ccacagggat cacctacctg
    20101 gacgccgtgg cagggcttgc agccgccagg atcgaggagg cggccaggtg cggggaggag
    20161 gttcgcgtcc aggtgaagat ggcaaaactc ccctccgatg tcaacctgcg tatcgaggac
    20221 tacgtgatgc gctacatcac agaccacaac aaaaaagtgg atctccgcgg tccggtcttt
    20281 ctgacggtca ggtcggtcat ccggaagccg gggggttaaa aggcttaaac caccacggtt
    20341 tatacctttt cggtctttct tgttcttatt gggaggccat caaaccagtt cgatcatggt
    20401 ttgacgccat cggatcacca ccgtggtgaa acgtcttttg aggctggcct cctccatccg
    20461 gtgggcgggc ggtgcatgat gacagatcct ggaaagccgt ggcagcgacg gaaacgggat
    20521 ctcagggaag tatctcaccg agaacgtggt aaggagatct cctccctggc agtgtatcga
    20581 gggggaatca cccccctggg ggtggggctg acggggaggg gggaggcgga gacgactccc
    20641 ccctctcctg tctcctgcgt ttcaaccgga cactcttttc cgtgtgaggt aaaaacaccc
    20701 ccaccgcccg atcgccccgg atccggattg gaagaggcgg gcggtgatgt gtccgccccc
    20761 ggaatccgtc cacacaggcc ggggcgcaac cccatgaacg ccgcaaaaga actccaattt
    20821 caggatattt tcttctggcg tctataaaca accgccccat ctctgaattc tgccccggcc
    20881 tgaggtattg gtttgatggc ttctactgca ctttatgagg cccctggcag gtgagcagac
    20941 gtctctggga ggcgaggttc aacctgaccc tggccgggtg cgaagagcgg taaggttatg
    21001 tatttctaac accatgttag aaatatacaa ctggtgtgac tatgaagcga acctacgctg
    21061 taaagatagg ggtgggagtg atcctcgcgg ctgcgggcat aatcctccca tttctcgtgg
    21121 acggaatgga atctctctcc tcggtcctgg ttgctatcgg tcttgtgacc gttgcggtga
    21181 ccggtgtgca gcactggaga tgcagcgacg atctggagaa ggacgagagg actcagaaac
    21241 tcggggcata cgccatctct tactcctggt tgctgaccct ggtcttcctt gcaatcctct
    21301 tctgggttga ctaccttggt ctgctcgtac tcagcgtcca ggctgttctg gtctccgcca
    21361 tcctgctgat ggggctctcc gcgcggctct tccagtggta cctattccgg cagggtgacg
    21421 tggcttgagg ggggatgccg ggtgaagacc cggatcaaag aatttcgggc gaaggccggg
    21481 atgacccagg aggaactggc caggcaggtg ggcgtccggc gggagacgat agtcttcctt
    21541 gagaagggga agtacaaccc ctccctgaaa cttgcctaca gggtggcaaa ggcgctcgac
    21601 tccaccatcg aggaggtctt catcttcgat gaggaggatc cggcatgaga acctcctctc
    21661 cggtcggtgg gaaggtcacg attgcggctt tatctcaccc ctctcatccc ccatttctgg
    21721 tataggcgcc tctatagttg ctgcagtcct gtcatctccc cggagaagcg caaacaaccg
    21781 ggcaagagag atgatatcct gcctgttatg ctctacgatc gggatgagcg ggccggggtt
    21841 acctgttcgc atgtaggtct cgtagaactc cggaaccatc gcgcccggga gatcgtctgt
    21901 ccgggttatg ttgaggactg ccgtctcaag actgccgaga cggcaggagg ataagcggcc
    21961 tccccagcac cggcgtgcaa actggaggac gtcaaagtgg gggaggtcga gggctgccgg
    22021 gacaccgtgc cgggcgagtc gatcccggat gtaggggagg tcaaaagccc ggccgttgaa
    22081 ggtgacgagg gcggctcttt ccgcctccag gtcggggagg atggcggtca gcgccgctgt
    22141 ctcctccttg atcgacctcg ggaggtgctg acggacgacg atagagcctc tttccacccg
    22201 gcccaggcct atcaggatga tcggcctgga ggagagcccc agcgtctcga tatccaggaa
    22261 gaggaaatct tccggggcgt gaaacctgct ggcctcgagg aggagcggat cgctctgccg
    22321 gtgccgccgt tcaatccagt tcacgagttc ccgcgtctcc ccccttgcca ccagatcgag
    22381 gagccggctg gcatcctgat ggtaacgggg gtgccccagc aggtcaggga cggtgcggta
    22441 accgcgctct tccagccgct gccgggttgc cgggccgata ccctggatca ggctgatatc
    22501 gcccaggatg gctgccgccg cgcgttcgcg gtctcgaacg gccaggggga tcgggcttcg
    22561 ggagtctatg acgtagcacc tcccgcctga gcaccgcacc tcccggccgt tgaagacgtc
    22621 ctcgatcgcc aggccccggc acctctcgat gagattggcc cggaacctgc gggcctcctg
    22681 gtagccggag tggtgaagat gccccttctg gagatcccgc acagaccacc tcacccgatc
    22741 aggtgcttga accatttcat cctgatctct gtctttgcat aaattcaacc tgactcccgc
    22801 ggggtgaaag tgaaccgccc cggcgcctga cagaccggtc accggaagcg agcgttttca
    22861 aaccggcatc atgtggtttt ttgcaattcg ccgtgcatac gacctggaaa aacctggcgg
    22921 gtttatattt cagatcggtg attcgatacc acgcgcatgt agcacctcct ccacctccgc
    22981 ggccatccac gtaaatactc tcagtgacaa tcgagaggag agagatgaat attggtgcag
    23041 tcctgatcga tatcgatggc gttctccacg taggtgaccg gccggttgaa ggtgccgggc
    23101 gggcgctccg ggaactcgac cggcgcggga taccctaccg gtctgtctcc agcacaaccc
    23161 gtagttccag gcgttcggtt gcgcagcgtc tgcatcgcct cggttatgag atcccggaac
    23221 cctggatcgt caccgccccg gctgcggccg ccgtagacct ccgggagagc gggcggacgc
    23281 ggtgctgcct tctgaccacc ccggatgccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn naccgcgaag aggccgtccg ccgctcaggc atcaccccgg acctggtcat
    23461 cgactcgctt gcagaagacc ttcccgacca tatatgatgg ccatgataaa gcggattctc
    23521 ttcctggttc tcctggccgc cgcaatcggc ggagggtgtc tcgggttttt cctccgtccg
    23581 gtgttcgtcc cgccggcgat cgtacccctg gagggtgcga gcctcaacca gacgccggtc
    23641 tataccttcg catttgagga cgggatggag acgatccgca tcgaccccga cccggcggtc
    23701 tacgccggtg cgaaagcggc agacaggagc ctccacctct acacggatat gccggaagaa
    23761 gagtggatcc cgatctacta cctggcgttc ataaacgagc cccaccagga acccttctat
    23821 gcggacctgc ttggcgccct tcgcgagatc cgtgaccgga gagggctcga tgacgaccgc
    23881 tacctcgaac tgatcgccgt attcgtgcag tctatcccct atgagaccga cgcctcggtg
    23941 accgaaccaa agtttccgat cgagaccttc gtggacgctg ctggagactg tgacgacaaa
    24001 agcctccttc tcgcagggct cctcgcgcgg gagggctacg gtgtttcgct cctctacttt
    24061 ggagacgaaa accacatggc tgtcggggtg aagagcgcag gctgcggcta ccgcaacacc
    24121 tcttttgcct acatcgagac cacaaacgta agttatgtcg gaatcccgcc tgcaacgctc
    24181 tcgggcggcg cgaccctctc ctccgacccg ctggtgatcc cggtcggaga aggtgaacgc
    24241 agctacaccg cctgcaacca ggttcagacg attgagcgcg ccctctccgc attacgcgac
    24301 cgtgccgcgg ttatcgagac ggaacttgag gcacgcggtc gggagatcga ggcgatgcgc
    24361 gatgccctcg acgccctgaa tgcccggatg acgccactcc tgcgtgcggg cgatatcagt
    24421 gagtacaacc gcctcgtcca ggactacaac aggatggccg gggactacaa ccaggctgta
    24481 gacgcttaca acgccctgct cgatgaggct gagagggttg tcgacctcca caacgacctg
    24541 gccgccggtg ctcatgaccg gcccgcctca tacatcatgg cgcgggccta cctctgcggg
    24601 tgacactcat ttaacccgga aaatggttgt gtgcgtctct ctgccccggg cgatgggcgg
    24661 cccacggcac catcggcagc aatatacacc tctttgcgaa tctggctgcg gcgggtgatg
    24721 gtcgggtagc gggcggcccg ctccgcgagg caggcaaacg tcgtgagttc tgatctctcc
    24781 atgcatgata acgatgcaat ctgtttattt aacgataggc cgcgcgcggt gtgaaagtga
    24841 aaatttgggt cttcaaatcc tggcctataa tggcccaaaa caccgtttaa tccttgatcc
    24901 ttattggtat ggttttttgg tctattcatt cgggtttaat tttggaaaac aaatttttcc
    24961 gtggcttgat atgagataag gggctgctgt caccgtatta tacctccggg ccagccgtcc
    25021 tgcaagtttt tatctatcct ctggcagata catgaaacgg agtgccgtat gctggatatc
    25081 agtgatttgc atgtggaagt tgatggcacc gaagtgctcc acgacatcaa cctccatatc
    25141 gggtgggggg agacccacgt tctgatgggc ccaaacggct cagggaagag cacgctgctc
    25201 aagaccatca tggggtttgg cggttacacc atcacatccg gttcaatcat ctttaaaggg
    25261 caggatatta cccggatgcc gatccatgag cgcgcccatc ttggtatagg catgatgttt
    25321 cagaacccgc cagcgatctc ggggctgaaa ctcgggaaac tgcttactgc catatccggg
    25381 ctgaaagagg acgaaatcga ggcactggcc cggtcggtca atatggggca gttcctcaac
    25441 cgggacatca acgtcggctt ctccggcggc gagatcaagc ggagcgaggt cctccagctg
    25501 aaggtccagc agcctgattt cctgatgctg gacgaacccg agagcggtgt tgacctggag
    25561 aacataaacc tgatgggcag ggagatcgcc accctgcttg agaaggatgt ccgtatcgtc
    25621 aaccggcgcc ggagcggcct gataatcacc cacaccggct acatactcga ttacgtcgat
    25681 gccgaccagg gtcatgtgat aatcgaaggc cagatcaggt gccacggaaa cccccgcgag
    25741 atcctgcgcc ttatcaaaga gaaaggatac ggagagtgtc tgcgttgcaa acagatatca
    25801 taactgacat ggaacacctc tcagagaagg accgggagcg tcttgccctt accggtctcg
    25861 aagtggagat gcggaatcgc tgcgggagtt tcttccagat cgaccagaac gttgtccaga
    25921 ccacatgcat ggcagagggt gtcgagatgc tccccatcaa ggccgctctc gagaagtatg
    25981 actgggtaaa ggactactac tggaaggccg tcccgagaga caaggataaa tacacccgct
    26041 acgtggcaga ccaggagaac ccccagggtc tcgttgtcat cgcccatcca ggcacaaaga
    26101 tcgagatgcc tctccaggca tgcctcttcc tccgggaggg cccggtgcag cacgtccaca
    26161 acatatttat agcgcaggag gactccgaga tccacatcat ctccggttgc gcgagttcct
    26221 ggcagaaaga gcatggagcg catatcggtg tgaccgagat ctacgtcggc aaaggcgcaa
    26281 aggtgacatc caccatgatc cacaactgga accgggggat cgccgtcttc ccgaggagcg
    26341 ccacaatagt cgaggaggga gggttgttcc tctcaaacta cgtctgcatg cagccggtca
    26401 ggaaggtcag catgtacccc acggccagcc tggtcggaga taacgctgtg gcccggttct
    26461 cgagcatcgt ggttgcgaca ccgggctcaa ccctggatct cggctcccgc gcgatcctcg
    26521 gcgccgaaca tacaagcgcc gaactcgtca cccgggcgat caccaccggc ggcaccatca
    26581 tctcccgcgg gcatatcctt ggcgcgaagg acaacacccg cggccacata gagtgcaggg
    26641 gcctgatcct gaaggacggc atcatccatg ccatccccga gatcgagggg acgatcacgg
    26701 gcaccgaact ctcgcacgag gccgcggtgg gaaagattgc ccgccacgag atcgagtacc
    26761 tgatggcacg cggcctctca gaggaggagg ccacggcaac catcatacgg gggttcctgg
    26821 acgtgaagat cagcggtctc cctgccgtcc tgcaggccca gatcgatgcc gcaatcgaca
    26881 aagccgaatc gggcttctaa ccgatgaggt gtgagatgac ggagatagac ccctttgcgg
    26941 gagcacgaga ggcgatggtg gagttccaga tccgggcgcg cggggtcagc gacccgaggg
    27001 tgcttgccgc aatgaggaag atcccccgtc acctctttgt gccggaagag ttgaggcacg
    27061 cggcctacga cgaccgcccc ctccctatcg gcgaggggca gacgatctct cagccctaca
    27121 tagtcgctgt catgacggag cagcttgaac tctcgccagg agaccgcgtc cttgagatcg
    27181 ggacaggcag cggttaccag gcggcaatac ttgcagaact tgccggcagc gtcatctcca
    27241 tagaacgcct cccccgcgtc gccgagaagg cgcgccagaa cctggcccgc gccggggtca
    27301 ccggcgtcga ggtggtagtc ggcgacggca cgaaaggcta ccctcctgcg gcgccgtatg
    27361 acgccatcat ggtcacggcg gcagcgcctg agatccccaa accacttatc gatcagatgg
    27421 ctgaaggcgg ccgactggtt gccccggtcg ggtcgcgcga ctaccaggaa ctgatcaaac
    27481 ttgtcaaacg cgggggcggg atcgagacga tcccccttgg cggggtctgt tttgtgccgt
    27541 tgatcgggca gtttggatgg cagggaggtt actcctcgtg accgtccacg acatcacccg
    27601 ggttctatcg cgggatgccg tcgtctaccc gggagacgtc cggccgcggt tccgcgagat
    27661 cgataacggg cagtaccgtg tgaccgagat gaccctcggc agccacaccg ggacgcacat
    27721 cgacgccccc tcgcattaca tcgagggagg ccggacggtg gatgagatcc cgccgggggt
    27781 gctggttggg ccggcgaggg ttctggactg cagcgatgcc ggagaggtca tcggccccgg
    27841 cagactgctc ggccgcctgg atggggtaag gactctcctc ttaaagacct ggttctcggg
    27901 gctgcgggag ttcgatcccg gctacccatc tctctccatg gaggcggcag acctgatcat
    27961 cgctgccggg atcacctgta tcgggacgga cgccccctcg atagaggggt tcagcggtga
    28021 cggttccgtc caccgccgat tgctcggaaa cggtgctgtc atcctcgaac tgctcgacct
    28081 ctctggcgtg gcagaagggg agtattttat ggcagcactg ccgctgctgc tcaggggtgg
    28141 ggatggatct cccctgagag cgctgctctg ggatgcggag gatgaagtat gagtttatgg
    28201 catgatgcag tcgagagact ggagaagatc ctcgctggca gcggctgcga ggatggtatt
    28261 gtaacgcttg gcgtaaacta cgacgcagac cgctgtcaat acccaaaagg ggtctgtatg
    28321 gaggctcagt ttggcggccg gagcgggcag ttcgtcacga acgaaccgac ccgggccagg
    28381 acccggatct cctttatgtt tggtgcgccg cttgcgacca cgaaacagcg ggcggcggca
    28441 ggcgcgatca tcaacgccgt ctcggcattt ctctgcctga cacggagatt gcagccctgc
    28501 acgaaggagt gtcacggccc atgccttgaa gccctctccc gcgaggtcgc cgggaaacgg
    28561 gtctaccttc tcgggccgat gcctcttctc gtgcgggcgc ttgaagcgca ggttgcagac
    28621 tctcccgaag aggccgatct catcctggtc gcaggggacg gcatggcaac cgatgccggg
    28681 gttgcctgca tcgacggcta cagggggagg aagcggatga tcttcattgg cccatccact
    28741 gccgggatca gcgggcttct cgacctcgaa cattggtgcc cctacgggag atgaatacat
    28801 ttttcaggca caaaggggct atagagagta tgctttttgg ctcttccggc attcggcagg
    28861 agttcggccc tgatctagtc gatcttgcgt tccgtatcgg atctgccgcc gctgcagggg
    28921 catctgagat cgccctcggt cgtgacaccc gcaccaccag cgagttgctt gagcacagtg
    28981 tagtagccgg gatgcttgcg accggtgcaa cggtctactc ctgcgggatt gcgcccacac
    29041 cgacggtcgc gtacgcgaca gggagcgccg atgcgggatg catgatcacc gcgtcacaca
    29101 accccgagcc ctacaacggc gtcaaactcc tcaacccgga tggctcctcg tttaccgtcc
    29161 ggcagcaggc cgcgcttgaa gaggcgctca acggaacaca ctggaagggg tgggatgaga
    29221 tgggtcgcct cctcccgatc gatgcgattg acgtgcacag ggaggcgatc ctggataagg
    29281 tcagcctctc tcatcccatc agggccgtgc tcgactgcgg caacggtgcc gggagcgtca
    29341 caacccccga tcttcttgca gaagcagggg ttgcgctcac cggcctgaac tgcaacgttg
    29401 cagggaagtt tgcacgaccg tctgagccgc ttgagtcaaa tctcccctac atcggtgaga
    29461 tcgtccggag gcgcggtgcc gcctgcgccg tcgtccatga cggcgatgcc gacaggatga
    29521 tggcgtttga tgagcggggc cgctacatag acggcgaccg ccttctcatg ctgtttgtga
    29581 agtatctcga ctgtaaacgg gtggtcacaa ccgtcgacgc ctcgatggcc atcgaggaga
    29641 ttgcagatgt ccgccgcaca cctgtcggcg acagtttcgt ctccgaagaa ctcatatcct
    29701 ggggcgattt cggcggcgag ccctccggga gctggatatt tcccgggcac tcactctgcc
    29761 ctgacggtgt ctacgccgcc gccctgctct gcgagatcgc atctgaatgc tcgatcgccg
    29821 aagaactcga tcggatgccc cgctacaccc tgctccgcag gtcatgcccg gttccagacc
    29881 cccgtcgggt catggcggcg atgggcgcgg aggaaccagc gcacggcatc cggtttgagg
    29941 acgaggacgg ctggtacctg gtccgggcaa gcggcaccga acccaagatc aggatcaccg
    30001 ccgaggggag aacaccggaa aaggcagaag agatgcttga agcagcgtcg gccgccctca
    30061 agcgggcgag acgcagtctg gagtagatgc tacatgcagt gtgttgtact tgcagctggc
    30121 gaggggaaac ggatgcgccc ccttaccgcc cggcgcccga aagtgatgct tccgatcgcc
    30181 aaccgcccga tgatcgagca cctgatcacc gccgcccgtg acgccgggat caccgggttc
    30241 atcctggtcg tcggctactg tgagcgtgag atccgcaacc acttcggtga cgggagcggt
    30301 cttggcgtaa agatcacata cgtgacccag cgcagacagt tgggcaccgc tgatgccctc
    30361 cgggcggcat cggggatggt gaacgatcgc ttcctcctct taaacggcga tatgattctg
    30421 agatcggatg atataaggag cctctgccgg atggaagccc cgtgcatggg gattcatgag
    30481 accgaccacc cgcaggacta cggcgtggtg acggtcgagg ctgaccaaat caccggtctt
    30541 gaagagaagt cggaagaacc gaagagcaac ctgatcaacg ctggcgccta catcttcgat
    30601 cccgatatat tcgaccttct ttcaggagtc ggggtctctg agcgcgggga gttcgagctg
    30661 accgacgccc tcgcatccta catagaggag ggaaggctcc ggggatacct ccttgactac
    30721 tggctcgatc tcggacaccc atgggacctc cttgacgcaa acgcagagat cctctcctcg
    30781 atgcaccacg aaaagcatgg tgtgatcgag cccgggtgca gcgtcccgga gaccgtcagc
    30841 atagggaggg ggacggtcat ccgggcgggg acatatatcg agggctcgtg catcatcggt
    30901 gagaactgcg ttatcggccc ccacgcctac atccggggtt caaccgccat cggtgatggc
    30961 tgccacatag gccacgcgac cgagatcaag aactccatca taatgtcggc gacaaaggtt
    31021 ccccatttca actacatagg cgacagcatc ataggaagcg ggtgcaactt cggggcgggc
    31081 accaaggttg caaacctccg gcatgacaac ggagcggtga aggtctgcgg gaagagcacc
    31141 ggccggagga agtttggcgc catcataggc gatgatgtgc tcttcggcat aaactgttcg
    31201 gtcaacgtcg ggacatccat cgggagcggc acgcgggtct ccccccactc ctttgtggac
    31261 gggtgcatcc aggagaactc ggtcatcagg tgatattatg caggcagtaa ttttagcagc
    31321 gggagagggg agccgtctgc gtcctctcac ccggagcaag ccaaaggctc taatcccggt
    31381 cgcaaaccgg ccgatcatcg attacgtcat cgatgcactg ctggagaacg ggatccgcga
    31441 gatcgttgtg gttgtgggat accgcaagga ggaggtcatc cagcacctca acagcctcga
    31501 cgcgccgctc caggtcgtcg tacaggaacg ccagctcggg acgggggacg ctctcagggc
    31561 ggccgagccg gagatcaggg gcgacttcct ggttctccct ggcgacaact acatcaatgc
    31621 cgggtcgatc gcccggatca agggcgtgcg caacgccatg ctggtggcag agcaccccaa
    31681 cccctccaac ttcggtgttg tggtgatccg cagcggcatc gtccgcgaga tagttgagaa
    31741 gcccgaagac gccccgacgt tcacggtatc gaccggggtc tactcattta caccggagat
    31801 cttctcctac ctccacacaa acgagatccc cgacgctgta ggtgccatga tagcagatgg
    31861 ccggcagatt cgcgcaatcc cggcagacga gtggcaggac gccatatacc cgtgggacct
    31921 cctgaaactg aacacccgga tgctccgggg gatccagccc gggatcgccg ggacggttga
    31981 tgcatccgtc gtccgccggg gggtcgtgca gatcggcaaa ggaaccaccg tgggtccaaa
    32041 caccgtgatc tacggccccg taaatatagg gagcaactgc aacataggcc cgaacaccgt
    32101 tataatgccg gacacgagca taggcgatcg ggtggtgctc gagcccttca cctacgttgc
    32161 tgactcgctc atcatgagtg atgtcacggt cgggtcgcac tcacggatag tctcggcggt
    32221 cttagggcag ggctgcatcc tcgccgacca tatcacaacc actccctctg caagcctgat
    32281 cgaggtgggg ggccgtgtcc agaaagagga gttcggcgcg gttctcgggg agggggtaag
    32341 ggctgcacca ttcacaacgt ttaagaactg catcgtcggt aacaatgtca gcatagtcaa
    32401 ggggcggatg ctggcgggca ggatcgagga cggcacaagg gtgatgtaga tgtgcgggat
    32461 cgtcggctac accgggaggc gggcggcaac ccctatcctg gtgcggggat tgaagaggct
    32521 tgagtaccgg ggctacgact ccttcgggat tgcaacggtc ggtagcgcga tcgaggtcta
    32581 caagaaggcc ggccgcatcc cggacagcgg ggtggtgccg gtcgatctcg cggggtgcac
    32641 cggtataggg catacccggt gggcgacgca tggtgaacca aacgagatca acgctcaccc
    32701 tcacgtggac tgtggcggga ggatcgccgt tgtgcacaac ggcgtgatcg agaactacgc
    32761 tgaactgaag cggaatctcc aggagagggg gcataccttc aggtccgaga ccgataccga
    32821 ggtgatcgcc cacctcatcg aggagcggta tgacggtgat ctcctggcag cggtcaacgc
    32881 ggttcttcca ctgctccggg gctcgtatgc aatacttgtg atcgcctgcg acagccagag
    32941 gatcgtcgcc gctcgcgacg caagcccgct tgtcctgggc atcggtgatg acgaggtctt
    33001 tgccgcttcg gacatgacac cgcttcttga gtacacggaa cgcgtgatct tccttgagga
    33061 cggtgatgtt gccgatctca ccccctgccg ctgcacaatc tatagcggcg gccggaaggt
    33121 tgagcgcccg gttgaactga tcaactggtg tcttgaggat acacggaagg gcggtttccc
    33181 ccactacatg ctgaaagaga tctacgagca gccccaatcg ttctacgaga ccctcagggc
    33241 aggtgtcgac gagcgtgtgc gaaagttggt gatggaggcc gatgagatca cgctggttgc
    33301 atgcgggaca tcctatcata ccaccctggc cttcaggtac ctggctgaat cgctctgcgg
    33361 cataccggtg cgcgtcgaga tgggttcgga gttcaagtac ttcacaccgc ccctccacgg
    33421 gcttgtgatc gcggtctcgc agtccggaga gacggcggac acgatcgccg ccctgaagat
    33481 ggcgaaagcc cgcaactgcc cgacgcttgc cataaccaac atgcagggaa gcacagtcac
    33541 caggatcgcg gacgagacca tgctcatgcg ggccggcccc gagatcggtg tcgctgcaac
    33601 caagtcctac atggcgcagc tcgcagcgtt gatgcagatc ctcaacatcc gctgcggcgg
    33661 cgagtttgac gacgccctct cgcatgcgca cctcgcgatc ggggaggtcc tgatccagga
    33721 tatacaggat gcggtcgatc tatgttcgag ggctgaacac gtcttcttcg tgggtagagg
    33781 gccgttttac gcggtggcgc tggagggcgc cctcaagatg aaggagatct cctacgttca
    33841 tgccgagggt tacgctgccg gagagttgaa gcacgggccg tttgccctgc tcaccccgga
    33901 gacgccggtg gttgcgatct gcacccccgg cccgacatac ggggtgatgg tctcgaacat
    33961 aaaggagatg aaggcgagga gagcgccgat catcgccatc ggggttgccg gagatactga
    34021 gctccccgag atcgtggaca tcttcatccc catcccgaac acacatcttc tggttcaggt
    34081 gctgactgcg tcggtcgtcc tgcagttgct tgcctaccac accgcctgcg ccctggatcg
    34141 ggacgtcgac aaaccgcgga acctggcaaa gagcgtgacc gttgaatgat agagtatgga
    34201 cgcaacgccc tcggcgatgg ggcgacgata tttgagccgg tgaccatcgg gtttccatcc
    34261 cgtgaccgga tgggcctgga ggactatccc ggtgcagcca tagggaagaa cgcgatcctc
    34321 cggtcgggga ccatcatcta ctgcgatgtg aggataggtg atgctttcca gaccggccat
    34381 aacgtgctga tccgtgaaaa gaccgtcatc ggcgaccgcg tggcggtcgg gacggcggcg
    34441 gtggtagagg gcgactgcac catcggcgac gacgtccgca tccagagcct ggtctacgtc
    34501 ccgaccggcg cccggatcgg cgaccgggtt tttataggcc caaacgccgt cctgacaaac
    34561 gaccgctacc cgccggggcc ccgtgagtct ctccgcggcc cggttttagg ggacgatgcc
    34621 gtcatcggtg caaatgcgac gatcctgcca ggggtcaggg ttggtgaggg tgcgtttgtc
    34681 gctgctggag cggttgtgac aagggatgtg ccgccggaga tgcttgcggt gggggcaccg
    34741 gcacggttcc gtgcgctccc tgctgaagcg aggcggtgcc ggtgatgatc cccgttgccc
    34801 ggccggtggt cggggaggag gagatatctg cagtcgccga agtgatgcgg tcagggatgc
    34861 ttgcccgcgg ttcggtcgtc gcggagtttg agtcccgttt tgcggactac tgcggtgccg
    34921 cccacgcgat cggcgtcaac tcgggcaccg ctgcgctgca tgctgcactc ctcgccgccg
    34981 gcatccgtcc gggagactcg gtgatagtcc cggcgttcac gttctttgcg acggcatcga
    35041 gcgtctctat gtgcggggca acaccgctct ttgcggatgt cgatccgcgg acgttcaaca
    35101 tcgaccctgc ttcggtcgag gcactcatca gcccggatac ccgggcggtg ataggcgtcc
    35161 acctcttcgg gcagcctttt gatctcgatg ccgtccgtgc gctctgcgat gattatggtc
    35221 ttatgctgat cgaggacgcc gcccaggcgc acggtgcgga gtatcacggg agacggaccg
    35281 gcagcctggc cgatataggg tgcttctcgt tttacccgac gaagaacatg accaccgggg
    35341 agggcggtat ggtcacgacc gatgacgagc gcatcgccga acgggtcaga ctcttcatag
    35401 atcacggcca gagccggaag tatctccaca ccgtcatcgg ctacaactac aggatgacca
    35461 acatcgccgc cgcgatcggg atggtgcagg tcgagcggct tgaggcgttc aacgaacgcc
    35521 ggatcgagaa cgcccgctac cttgaccgcc acctggcggg gacggggctc gtgacgcccc
    35581 acgttgcgcc gggagtccgg cacgtctacc accagtacgt tgttaaactc ccggccggct
    35641 acccgcttgc gcgggaggcg ttcataagcg cccttgcgga acgtggggtc gggacggccg
    35701 ttcattaccc catcccgctc caccggcagc ctgtctacga gggtgcgggg aagaccgcgt
    35761 cctgcccggt atcggaagac cttgcggcat cggtcgtgag cctgccggtt cacccatcgg
    35821 tgacggatga agaacgggca tacatatgtg atgcggttgg gggattccag gccctgaaac
    35881 aaggccaatg atacaaatga ggatcctgct cgtatccacc caggattaca tccaccaccc
    35941 gataccatca aggcaccaca acatatttga ggagctggcg aagcggcacg aggtccatgt
    36001 gcctcacttc cacgtcagca gaggaaagga gcgcgagacc cttctccatg tccacgaggc
    36061 gacgtggctc ccgatcaaaa accccatcct tcactacacc ttgaacgcac cctgccacta
    36121 cagggtcatc agcagcatca tccgcgacca cgagatcgat gtcgccgtcg gggcgcacgt
    36181 cctggccggg acggccatgg tcagggcagc tgagaggtac ggcgtcccgg tggtcttcga
    36241 cctcaaggac tggttcccgg actcggccgc tgcctactac aaaaatcccg ctataaaatg
    36301 ggtgctccgt gagggtgtcc ttgccataac ccgctacaac ctggaccata gcgatctgat
    36361 cacgactgtc tccccgggtc tcgtcgagaa actcaggagt tacggctacg aggcggagtt
    36421 gatcacaaac ggtgtcaaca cggatatctt ccaccccatg gatgggagtg caatgcgatc
    36481 cgcactcgga atcgcgcccg atgccttcgt gctcgggttt gccggggcaa tcgaacactg
    36541 gtatgctctc gatgaggtga tccggctctt tccggaggtt ctcaagcggc acccgaacgc
    36601 cgagctcctg atagtcggcg gttcgctctt caccggttac atggacgaac tgcaggcgct
    36661 tgctgccagg ctcggggttt cggggaaggt gcatttcaca ggcaccgtcg actacaatga
    36721 cctcccccgc tgcatcgcgc cgatggacct ctgcctcatc ccgctctcgc cccctcaatg
    36781 ggtggatatc gccctgccaa acaagtactt cgagtactcg gcgtgcggga agccgatcct
    36841 ctcgaccccc atccccgata tgctcaggat gggcggggac catatcaccg tctacaggga
    36901 cgggcaggag ttccttgagg gggtggatga ggccgtcatg aacccgcggt gctgcacaat
    36961 cccgatagaa gagcacgcct ggaagaagaa agcagaggct ttcgagagga tctttacccg
    37021 gctgacggga aagacctgaa actgcaccct cccccatcca ccatcgatac accaagatta
    37081 tattggttcg aatggaaact gattcctaaa gggagtgccc gatggctcag atggatatca
    37141 agaaatacca accatatatc atcattggtc tcatcgtcat cctcgcgctg ctcacgctct
    37201 ggacgcgggg aatccccgca gagggactgg tgaccgacga gggtgtaaac ctgctgggaa
    37261 acgatccctg gtacaatctc cgccaggttg agcagacggt ggcaaacttc cctgcctacg
    37321 cctggtttga cgcgatgacg ctttacccga ccggggacat gatctactgg gggcctctct
    37381 tcatcgagat catatccgtg ttctgtctcc ttgccggggc aacgacacgg cctgagatca
    37441 tgctggtggc gtcctgggtc ccgccgctga tggccgtggt catggtgccg gtcgtctacc
    37501 tgctggcgcg aaagattgct gactggaaga cagggctcat cgctgccggt cttattgcgg
    37561 tcatcggcag caactacgcc taccgctccc tctttggctt tgtcgatcac cacatcgcag
    37621 agacgctctt ctcgacgatc tttgttctcg cctacgtgac ggcgctgctt gcggcgcggg
    37681 gggccacaat ctcgcgggcg aaccttcagg gcctcaagat ccctgctata acagcgatca
    37741 tcgccggggt agcctacctc cttggctact ttaccatgcc gacgatgatc ctctttgcgc
    37801 tcatcgtcgc agtgttcacg cttgtccagt tcatactgga ctacttccag ggtaggtcga
    37861 gcaactacct ggtctttgta aacgccgtcg tctttatagt ggttatcatt ggaaccgtgg
    37921 cgttcgggtt cccgcatccg gggcttgacc tctcgcgcta caccatcgga cacgtcatag
    37981 ccagcgctgc cctgatcgcg ggaacagcag cactctatgg gctctcggtc ttcctgaaag
    38041 agcggcctaa atattatttc cctctcgcgc tcctcgccat tgcagccgtc gtcaccgctg
    38101 tgctctatgt cgccctgcct gagctctaca acctcctcat ctcaagcctc atctccttct
    38161 tcggcgagca gcccgtaacc acaaccgtcc aggaggcgcg ggcatggtcg tttgacgccg
    38221 cctgggcgac cttccactgg gggctgctgc ttgctgccgg cggagcagcc accctgatct
    38281 ggcagaaccg caaggaggtg aaccccgctc acacattcgt cctggtctgg acagcgatca
    38341 tcctggggtc gaccgccgcg catgtccgct acgagtacta ccttgccgta aacatcgctc
    38401 tcctctcggc ggtgtttgca ggcgcggtcc tgggcgcaac ctggaaagag gttgccctcc
    38461 tcctccggaa aggaagtgca gacagcgcgc gccccgcggc gggaagagcc gaaaaacggg
    38521 aagagcctcc aaagaagaag agcaagaaag gggcaagggc cgctgatgct cctaaagcga
    38581 aatccaggaa gcgtgatcag cctgattacc tcaaagtcgg cgggtttgtt ctggttgctg
    38641 ctctcaccct cctctttgcc gggacgtcat tccaggggaa cctggatctc gcaaacagcg
    38701 cgaaatacag tggtatggac tcgcagtgga tggaggccct ggagtggatg ggagagaaca
    38761 caccagatcc cggcgtcgat tactacgcga tctacgatcc ggagaccttc acctaccccg
    38821 aagagtcata cggcgtcatg tcctggtggg actacggcca ctggatcacg tttatctcga
    38881 aacggatccc gaacaacaac cccttccagc acggcgttgc agggccgaac ggctcggcgg
    38941 tctatctcgt ctcgacatcc gaggaagccg caaaccgcat cctggacaac atcggtacac
    39001 gatacgtggt gaccgactac caggtcgtca tgggcaagtt ccacgccccg gcaacctggg
    39061 ctgatccgaa cgacactgga ggactgcggt tccagccggt attcttcgtt cccatgggtt
    39121 cgggatcggc gggctaccag gcgatgcagt tctacaacca ggactactac atgacgatgg
    39181 tctcccgtct ccacaacttc gatggctcca tggccgaacc gacaacggga tcggtgctat
    39241 acgtcgagta cgccgaaccg gaaatggcaa acgcctcata cccggtcatc accagcgcac
    39301 aggagatgaa cgccaccgcg gcggccgcag cagcagcagc ctataaccag aatgcaccgg
    39361 cggggtctca cgccgccgtc ctgaacctgt actacgagta ccaggctgac tcggtcctgc
    39421 atccgcttga gcgggtgccg gcgctcaaac attaccggct tgtccacgag accccccaga
    39481 acatctacgg caacgtgggc gaggccggcc ctgacctcaa ggcggtcaag atatttgagt
    39541 atgttcccgg agccgcaata aggggtgagg gggtcatcga ggtcccgata gccacaaaca
    39601 ccggccggga gtttgtatac cggcaggaga gcgagaacgg tctgtttgtc gttccatacg
    39661 ccacctccgg atggtcgggc gaggttagag caaccgggcc ctaccggatc gccggcaccg
    39721 gccagacctt cgatgtgact gaagaggata tccagcaggg gcgcaccatc aactgacagg
    39781 aatgcgctac tcgataatat acggtgacat attcacccgg cacgatattg aggcccaccc
    39841 cgagtcggcc gcccggttgc gggcggccct cgactgcata cccccgagcg cacgctggcg
    39901 cccccccatc cccgcctgcg aggccgatct cgagcgcgtc cacgacccgc attacatccg
    39961 ctgggtgcgg gagatggcga cgggcgcctg ttttctagac gcaaacacct atgtcacctg
    40021 ccactctttt gaggcagcgg cccacgctgc cgggtccacc attgcagcgg cggagcggac
    40081 gcttgatggt gagcactgtt ttgcacttgt ccgcccgccg gggcatcatg ccgagcccga
    40141 ccgcgcaatg gggttctgca tattcaacaa cgccgcggtt gcggcggcaa aagcgctcct
    40201 gtccctcgac cgggtggcga tcctggactg ggaccttcac cacggcaacg gcacccagac
    40261 gatcttttac gggagcgaca gggtgctctt ctgctcggtg caccaggaga acagtttccc
    40321 ctggacgggc tgggtggacg agatcggcac cggcgccggc cggggctaca acctgaatgc
    40381 accgcttgcg gcaggctcga ccctcgccga ctacgccctg gtattcgagg aggtgttcat
    40441 ccctgctatc gttcggttcc gcccggatct tctgatagtc tcggccgggc aggacgctct
    40501 cgccgatgat gggcacggcg ggatgaaact tgaacccgac gactacggtg tgcttgccgg
    40561 gatgctgata gacgggacgg acctccccct ggcgctgacg ctggaaggag gctacggccc
    40621 ctcggccggg aaggccgtag gcgctatatt ccaggccctc gcggggagac ggtttgagcc
    40681 ggcagaaaag ccggtgcacc ggagcacgga gcgggttgtg cggatcttaa agagagtcca
    40741 gttctgttga gcgccgccgc ggtcgggagt tacattacgg tagggttaac cccccaccac
    40801 gggatgaccg ccgccggcgg gggtccatgc aggagacctc gaaaccgggg atgatcaccc
    40861 tcaccacggg gatcgcggtt ctggtgaggt cgcagacgca gaccctgtcg gtgtggggcg
    40921 cgacctcatc tagtacacgc cggatgtcca ggtcgaaccg tcctgtgctc atatcaggga
    40981 tatcagcgag gtcgatggca tcagcgttgc caaaccagat cctgttgatc cgtttcagcc
    41041 gttcataacc cgccttcctg attaccgcct cccgccggat gtcgccaggt tcgccgtgga
    41101 gaatactccc ccttctcttt gcaacctccg tgagcgcctg gaacgctgca gcctcgggtg
    41161 agaggtggga ggcagacccc gtgaggatca tcgcaggatc ccgtgtaacg gtgtcgtctg
    41221 cggtggccgc gactgtgggg atctccgttc tcccatcgag cagccagagg tgaatctcga
    41281 tcccattctg ctcgaaccgc tccagcaggc cgcgggcggc gcaatccttc tcgatggtga
    41341 ggcggcggcc ggggtttcgc cctgcatcag cgatgctgag cgcgtcccgc tcgataacct
    41401 cgaggagtgc gtggaaaatg gcctcttcca tgacgtttcc cgccgcaagc ccgtttgtgt
    41461 cgctccgaaa cagcggtgtc gtcaacccga gcgagtcgta ggggtgaaag acggcgttgc
    41521 tcgggacgta gagatcctcg tcgttcagga tgtccctggc cggtgtccag tggaccacct
    41581 ccccctgttc gagttcccgg ggaagaatga ggtcttcggg gtgcagtgcc cgtgcagggc
    41641 cgatctcctc gtaggaggcg taatccatgt ggtcaccatg gtattcggcg cagcagcgct
    41701 cgatcgccgc catcatcgcc gacacccgtg cctggaccgc ctctttacct gctcctgtgt
    41761 aaacccgaac cgctccccgc gccgctcccg gccggactgc cgcaaataca gggatcccgg
    41821 tccggtcgag cggggttata tccgtgatct ctgtaacccc gatcccgctc ataaggggtt
    41881 tcacaacggc gtccgtctcc cccggggagc gtgaccggtg gccgccatca aagtagacct
    41941 tctctacagg gcgaatcgtg atcggcattc atgatccctt cgtggtaata ccttaatagt
    42001 gtggcagatc tattctagca tgttatgagc acggatgata ttgcagttgg tgcagtcgtc
    42061 cggtaccccc gtaccggcac gaccggaaag gtcgtaaaga tcgaggagat cgatggccgg
    42121 gagtatgctg aaatagatgc caccggcctt tactacctgc tggatgaact tatcaggaca
    42181 gaattgacgg aggtagaggc caggaagaag gagcgctccc tcgaggacta catgagggag
    42241 cacagggaga tccaggagca gctggagaag gtctgggagg ttggaaccga ccagagctgc
    42301 gagggcggcg gctaaccctc gatcggtatg gtggtgagtt tcaccgcctc aacacccttc
    42361 tgtgccataa gtttctcggt aatatctttc agtatggttc catcccccct gaccaggata
    42421 acctcaagac atttgctgtg cgttacatgc gagtggagtg aggcctgaat gttgccggta
    42481 tactggtgct gtatgtcgat gagcgtctgg agcagtccgc gctggtcatg gtcatagacc
    42541 atggttatga cgccctggcg ctcgcctttt acgtctgata tccactggta gtgagtgatg
    42601 taattccgta tcgagtcccg tatcccctcg gaccgggaag agtagccccg gagtgccagg
    42661 atctcatcaa acttatcgag gaggctcttt ggaagcgaga tccctatgcg tgatagttcg
    42721 gtatcacctg acatggtgtg atcagttccc ttatcaccct tcataggtat aaattttcgc
    42781 taacaatcat ggtgatattc acacttcgta tgctgaaagc ggtacggcaa acggggtgcg
    42841 ggccgatgca tggtgtgaag acgcggcaag ggattacgag agcaatcgct accgattatg
    42901 tataagtagt gtcattctat aatgagtaag gaggttcgaa aacttttgcg agatgtattc
    42961 attccgggtg tcaatattgg gcttgtaggt catgtcgatc acggcaagac cactctggtc
    43021 agcgcgctga ccggcacctg gacagaccgg cacagcgagg agatcaagcg cggcatctcc
    43081 atccgcctcg ggtatgcgga cgcaacattt taccggtgtg agaaatgtaa gggcagcgac
    43141 gcttacacca cctccgcgga atgcccaaac tgcgggggga aagccgtccc gttacggacg
    43201 gtatcttttg tcgatgcacc cggtcacgag acgcttatgg cgacgatgct ctcgggttcg
    43261 gcgttgatgg acggcgcgat gcttgttata gctgcaaacg agtcctgccc ccagccccag
    43321 acaaaggagc acctgatggc gcttgagttg atcgggataa acaggatcgt catcgtccag
    43381 aacaagatcg atgttgtgac ccaggcggag gcgctggagc attataagca gatcaagcgg
    43441 ttcatcaagg gcactatcgc cgagaacgct cctatcatcc cggtgtctgc acagaaaggt
    43501 gtcaatatcg gtgccctgat ccagacgctg gatgaggtca tcccggagcc tgaacgcaac
    43561 cctgatctcg acccgctgct cctcattgca cgctcgttcg acatcaacag accgggctgc
    43621 aactggcggg acgtgaaagg cggtgtcatc ggcgggtcgc tcatccgggg ggtgctgcgt
    43681 gagggtgatg agatcgagat ccggcccggc cggcagttgc aggtcgagaa ccggactaag
    43741 tgggagccta tcacaacaaa gatcacctcg atcaacgccg gcaggatcaa tgtgaccgag
    43801 gctagtccgg ggggtcttct cggcatcgcg accaaacttg atcccgcact gacgaagagc
    43861 gacgctctcg ccgggcaggt tgcggggctc gtcgggagac tgccgcccgt ccgggagcgg
    43921 ctgaagtttg atgtaacgct gatggatcgg gttgtcggtg cggacagcga gcagataatc
    43981 gagcctctca agcataaaga gccgctgatg ctcgcggttg gcaccgcggt caccgtcggc
    44041 gtgatcacga acaccagaaa gaatcaggtc gaggttcagc tgaagcgggc ggtctgcgcg
    44101 gagactggtg cgaggattgc cataagcagg caggtcggcg ggcgatggcg gttgatcggc
    44161 atgggtgttc tggtcgagtg aaggtgcttc tcgacacaaa cgctctcctt atgccggttc
    44221 agttcggtat cgatctctat gacgggctga tggcgctcct gggggatttc gaaccggtaa
    44281 cgctcgaaga ggtggtcggt gaactctccg ggcttgccag ggggcacggc cgcgatgcgg
    44341 ctgccgcacg tgtgggtctt gagatggccc ggcgcgcgac gatcgtgccg agcgggagca
    44401 gcgcggagtc tgtggatggc cgggtgatcg agtatgcccg gcgggaggga tgcatcgtgg
    44461 ttacaaacga tcgcaacctc cgcaacgatc ttattcgtga ggggatcggt gtcgtctcga
    44521 tgcggaagga aagaacgctg gaactgatga ggggataggt gaaaatcatg tactataaag
    44581 tgacgctgga ggacaaggtg cgggttccgc cgcaccgcct tggggaagac cttgagaggg
    44641 ttatcctcaa cgtactgcag gaacagctgg aaggcagcat ctcaaaggag atcgggatat
    44701 tcattgcggt gacgggtatt ctcgatgtcg gcgagggaga gttgatcccc ggggacggcg
    44761 cggtctacta cgatgtaagg ttcgaggcga tcgttctccg cctggcgctt caggaggtga
    44821 tcgagggcga ggttgtggag acgacaagtt tcggcgcctt cgtcagcctg ggcccgatcg
    44881 acgcgatgct gcacgtgagc cagatatccg atgagtacat caactacgat gagaagaacg
    44941 gcaggctgat ctgccaggat tcaaagcgtt cgattgccgc cggcgacggc gtccggggca
    45001 ggattgttgc cctctcgtta aacgagcgtg agccaagaga gagcaagatc ggtctcacca
    45061 tgcgtcagcc gggcctgggc gcacttgcat ggctggatga ggagttcgag gaggagaaga
    45121 agaggaggtc ggcgtaagat ggccgtgggt ggtaagaagg tggtcagggt ctgccgcgag
    45181 tgccaccgcg tcgttgaggg ggaggcctgt gtgatctgcg gcacctccaa tctgagcgag
    45241 gactgggccg gatacgttgt gataatcgat ccggagcact cggatattgc aaagaagatg
    45301 aacatcaccc tgcctggccg ttatgctctg aaggtccgct gatgctcagg cttccggaag
    45361 cccaccggga actcttcaaa aaaccgtttg gcgtccttta tggaagtgtc gccgaactcc
    45421 ttcccctgat cgaggggcgg gtggtctgcg ccgtcggtga tgtggtgacc cataacctcc
    45481 ttgctgcggg ggttgtcccc gatatcgcca tcatcgacgg ctacacgatg cgcagcccct
    45541 gcaaccgttc gcccctgctc agagggagaa gggtgacggt gaagaaccct cccggaacta
    45601 tcaccgacga cctggtcgcg gctatcgatg aagcggtgcg gaaccggcct gtggtcatct
    45661 ttgtcgaggg tgaggaggat ctcgcggtaa ttccgctggt ttttgccgcg ccgcccgggg
    45721 ttgccgtgct atacggtcag ccgggggagg gggttgtcct ccggattgtg gatgaggcgg
    45781 ctaagcagga agcggcggcc atgctggagc gtttcatacg ggagtgatgg cttgcggctc
    45841 tctctccccg cgctccatcc cagagtataa ataaaccagc caacaatatt ttagggatat
    45901 cgatggagtt caaaatcacg cgtgatgtga ggaacgaggt cttaaagagg agagaacttg
    45961 agtttgttct cactttcaac ggaccgacgc cctcgcggaa gagcattcag gagaaacttg
    46021 ccgcactgca gaacagggat gagggtctca tcgtgctcga cctggagaga acccggttcg
    46081 ggaagatgga acttctcggc cgtgcccgga tctatgacga cgaggagacg aaggtctcaa
    46141 ccgagcggga gtacctgctc aagcgcggca aaccgaaggc tgagagcgag gcttgaagag
    46201 catggcgtcg aagagatacg agtgctacga ggtcaagggc aatgcggcgg ttctgctcaa
    46261 gcggcactgt ccccgatgcg gtccaggcgt cctgatggcc gagcacgagg atcgtgtggc
    46321 ctgcggcagg tgcggctaca ccgaattccg taagtaggcc ttcaccccta tatacccata
    46381 cacggccgcg ggcacggtat tttacgggcc gcggtcggtc ttttccttgt aacggagcag
    46441 gacctgttct atgtctgata aacgacctct cgaggggctg gtgctggggc ttgagggaac
    46501 ggcgtggaac ctcagcgccg ctctcttcgg ggaggaacct gccgccctcc attcgtcacc
    46561 atacgtcccc tcaaagggcg gaattcatcc gcgggaggct gcgcagcacc acgcttcggt
    46621 gatgaaggat ctcatatccc gggtgctgac cgaccctgaa cggatccggg cggttgcctt
    46681 ctcacagggg ccgggtctcg gcccgtctct acggacggtg gcgacagcgg cgcgtgccct
    46741 ctcgatagcc ctgggcgtgc cgctcatcgg tgtcaaccac tgtgtggcac acatcgagat
    46801 cgggagatgg gcgaccgggt tttccgatcc catcgttctc tacgcgagcg gtgcaaacac
    46861 ccaggtgctc ggttacctca atggccgcta ccggatattt ggggagacgc tggatattgg
    46921 tctaggaaac gcgctcgata agtttgcacg ctaccacgat ctgccgcatc ccggaggccc
    46981 tgccatcgag agcctggcgc gcagcggcgg ctacatcgaa ctcccctaca ccgtgaaggg
    47041 aatggatctt gccttctccg ggctggtcag cgccgcccag gagagcaccg cccccctgga
    47101 ggatgtatgt tacagcctcc aggagaccgc gtttgcgatg tgtgtcgagg ttacggagcg
    47161 ggcgcttgcc cacgccggga aggatgaggt gatccttgtc ggcggggtgg gtgcaaacag
    47221 ccggcttcag gagatgctcc gggttatgtg cgaggagcgg ggggcgaagt tcgccgtccc
    47281 cgagcggact tacctcggcg acaacggcgc gatgattgct tacacgggca ggatcatgct
    47341 ggaccacggt gtcacccttc ctctcgaaga gtcgcagatc cggccgggtt atagggccga
    47401 tgaggtggat gtcgtctgga ggacggaacc tggcgagatc tttgccggcg ggccgcacga
    47461 aggcggggtt gcccggggtg cagaaggtgt cgtggagatt ggtgaggata ctgttgccaa
    47521 gcgccggttg agcaaacgat accggcaccc ggcattggat cgccgtttga tcactgaacg
    47581 gacgcgggcg gaggcgcgcc tgatatcgat ggcgcggcgg gcgggtgtcc ccacgcccgt
    47641 gatccgcgat ataacgactg acacgatcgt gatggagcgg attcgtggcg atctgttgaa
    47701 gtatgtcgcc gcaccggaga cgatccgggt cgccggcgag acggttggga ggctgcacgc
    47761 cgcagggata gtccacggcg acctgacgac cagcaacatg atcgtccgtg acggccagtg
    47821 cgtactgatc gatttcgggc ttgcctctac atcatcggag gtcgagagcc gcggggttga
    47881 tatccacgtc ttcttccaga cgcttgagag caccaccgcc aactccgggg aactgatcga
    47941 ggcgttcatc gagggctact ccggcgtctt tcccgacgcg gatgaggtta ttgagcgaga
    48001 gcacgagatt gagctgcggg ggcgttacct ctgaagatca cggtggtgac gagcaacgcc
    48061 aacaaggctc gggaggttgc ggcatacttt gagggggtgc tcgaggttga gcatgccgcg
    48121 ctggagtgcc cggagttccg gcataacgat ctcggggaga tcgcccgcgg gaaggcggag
    48181 tttgcctacg agagactctc aaggcctctg atagttgacg acaccggtct attcatcgat
    48241 gcgctcggcg ggttcccggg gccatatgcc gcctacgtcc aggatacgat cggcaacgcg
    48301 gggatcctga aactcatgga gggcgtcgag gagaggagcg ccagattcga gacggcgatc
    48361 gcgtttgcac gggaggacgg catccgtatc ttccggggcg cccttcccgg ggcgatcgtt
    48421 gccccgcgtg gggcggaggg gttcggctat gaccccatat ttgagtacgc ggggcagacg
    48481 cttgccgaga tgccgcttgc cgagaagaac cgggtctcac accgggcgcg cgctctccag
    48541 gcgttccggg cctggatcga gcaggagacc ggagagcgac agaaccgtta agagggaata
    48601 aaggtaaatg acataaagct ggtgttttaa tatggcgaga tttcccgaag ctgaagcacg
    48661 tcttctcaat gtaaagatct gcatgaaatg caatgcccga aacgcagtcc gcgcgacaca
    48721 ctgccgcaag tgcggttcag acgagctcag gccgaagtcc cgggagcgta aagcgtaaag
    48781 accggtgcgc ggctttcgcg cgcctccggt atactttttt tatggggggg catcgccttc
    48841 ttcaggcgga gtagtaggtg acctttcggc cttcttcgct gtactcgccc ctgaaggtct
    48901 cgatgttgcg tttgtagccg atccgctctt tgaatccaag ttcgcggagc ctttcgcgga
    48961 cccgcatcac gtccgcctcg tccgcccagt cctttgtgta gacgtagatg accgtccgct
    49021 cgtcgcgaga gtctggatcg ggtctggcgg tgctgacctt cgcggatatg ccgagtctcc
    49081 cctcaacggt ctcgtttcga acctttcgcc acgcctcgtc cgcctcccct gcagggacga
    49141 agatgagcca ttttccagcc ctctcgtcgt cgaccccccg ctcatgcgga cccggtgcgt
    49201 cctggactat ccagtaagtt cttgtagtct ttgaggggga aactccctcg ccctctctca
    49261 gaggagcata tatggcgtcg atgctcccga accgtttgag cagggcatct gcgagttcgg
    49321 ggtaatcctc tctgaaacgg ctgaagacct cacggagttc ctctttgaaa tcggtcccct
    49381 gctcgacgag ttcgaagagg taagggccgc ggcgcctgag ttcgcggttg aggtagatct
    49441 caaagatccc gtagactgca tcggccaggg cttccggatc gatcccgctc atacataact
    49501 gtagccgccg gaacggtgaa aatggttttg aagttaccgg tcaagcgctt ctgcaacgag
    49561 ttctgcggcc cgctcgccgg agagaagcat cccgccaaag atcggcccca tgcggcactc
    49621 cccggcaacg gcgtttgccg ccataccggc gacaaagagg ccggggtaga cctcttttgt
    49681 gtgctccaga atccgtgact cggcacgctc ggcccacatg aaactctcgc ctttgacctg
    49741 gatgttgccg cctttacgct cgatcatccg ggcgatcgtt gcatcgtgcc ccgttgcatc
    49801 gacggtgcag gtgcaggcga tggtgagcgg gtcaacgtgc agtccggcca tatctaccgg
    49861 tgtccagttg atgaccaggc ccccaaccct gccgtcgccg cgaaccatca cgtcctcgac
    49921 cgtgaccagg ttgaagaact ctacaccggc atcgcaggcc gccgcggtga gtttggcaac
    49981 cgtctcgacc gatttcgcaa cgtagtagcc ctcctcaaac tcgcggcagg cgactccaaa
    50041 ccggtcgagc agccgccttg cctcttcctg gacgacaatc cttggaaaca tcatcccgcc
    50101 gccccacatc ccgccgccta tgctgagttt cttctcgatg agggcgcacc ggattccccg
    50161 ttcgccgagg agcgcggcgc atgtgagccc tgaaggcccg ccgccgataa cggcggcgtc
    50221 cacctcaagg aggtcgatga gcgcccggtg ctgctcttcc aggattgccc tgctgatggt
    50281 cacttcgttc agtctcatgt ccttcattta tatctgccag ggggagataa gggcaccgcc
    50341 gtgtgccacg aaaaaccgct ggatcgcggt ttggagcgcg agcgcccgga aagatcccga
    50401 cgaaggtctt aatagcggca ctgcacaaca tataccctac catgaagtgg acgcgcgatt
    50461 tcggtctcac tatgaggatg atcctcacgt cgctccttct cctcatagtc tacctgatct
    50521 tccttggcat ccttgccgcg ctcgggttcc cgtttgagtc cctcctgctg gtggctgccc
    50581 ttatggcgtt cctccagtac tacttctcag ataaactggt gctctggagc accaggacga
    50641 gaatcgtcgg ggaggacgag tacccggagt tgcaccggat ggtagagagc cttgccgcca
    50701 gggcgggtct tcccaaaccg aaggtcggga ttatggtgtc cccggtgccg aacgcgtttg
    50761 ctaccgggcg cagcccgaag aacgcggttg tggcggtaac cgactcgatc atgcggacgc
    50821 tcaaccgcga ggagcttgag gcggtgctcg cccacgagat atctcatgtg aagaaccggg
    50881 atatgctgac cctgacgatg gcgagtttca tatcgatgct tgccttcctg atcatgcgta
    50941 actggatctt cataagcctc ttcaacgacc gcgacaacaa catgggcgcg ttgatactgg
    51001 tctacgtggt ctcgatcgtt gtctggctgg tgagcaccct tctcacgcgg gccctctccc
    51061 gctacaggga gtttgccgcg gaccgtggca gtgcagtcct gacggggaac ccccgggcgc
    51121 tgatatcggc gctgacgaag atcagcggcc ggatggacta catcccggcc gagaagaagc
    51181 aggagattga gggggcaaac gccttcttca tcatcccggc cctctcggga aacaccctta
    51241 tggaactctt ctccacccac ccgcccctcg agaagcgggt ggctgccctc caggaactgg
    51301 aggcgcagat gcggggctac tgaccccgcc tctccaaagc ccacccgatc agcgatctgg
    51361 actataaact ctccgcgttt ttcctggaag aatgccgccg gcg
//
LOCUS       DAOSZX010000003        44332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_706_length_44332_cov_20.540958, whole genome shotgun sequence.
ACCESSION   DAOSZX010000003 DAOSZX010000000
VERSION     DAOSZX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 44332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44332
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_706_length_44332_cov_20.540958"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(582..800)
                     /locus_tag="PK196_00570"
     CDS             complement(582..800)
                     /locus_tag="PK196_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39950.1"
                     /translation="MDRGSKTDWIRIRQRRVIDGQDYYADRLYETQQAAWTYARQLET
                     RGATDVRVLRTATEGGYHGYAAFVKRDR"
     gene            complement(862..1143)
                     /locus_tag="PK196_00575"
     CDS             complement(862..1143)
                     /locus_tag="PK196_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39951.1"
                     /translation="MARRDVTLDEAREMGEKLGITWDEFDLEEFRRGMVVELEHGLRD
                     PVTNVTDDDLLMTAKIALAHLNEFPDYYERLEEMEEEAEAYWAERGAKH"
     gene            1341..2267
                     /locus_tag="PK196_00580"
     CDS             1341..2267
                     /locus_tag="PK196_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HON39952.1"
                     /translation="MTTVIAVDLGGTNLRAALVDSDANILAHETVPTPGAGRSGEMIT
                     DAIMARVETLLALPEGKDALAIGVASVGPLDLGRGCVINSPNIAFPLVEIVEPLRKRF
                     GRPVFLINDARAGVLGERWIGAARGSENAVYITLSTGIGGGAVVNGSLLLGKDGNAGE
                     IGHIPVDTRCNLICGCGFAGHWEAYASAKNIPTFFAAWQEAAGLRQVSFDTCSTQAIF
                     EAAREEDAVALAFVEALGKVNARGVSGVIVAYNPEIIVFDGPLARYHGDIVIRYMEPF
                     IDRYLTLPRLAVSSLDGKAPLFGAALYALEAL"
     gene            2285..2800
                     /locus_tag="PK196_00585"
     CDS             2285..2800
                     /locus_tag="PK196_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HON39953.1"
                     /translation="MIEIYPNLYIGSQEDYEVVVEAHDTWCVVHACRNPYHCLAVTYS
                     PLGTVPADHPEHLVARRGNRLMLNLVDSRDPADIPKEAIDAALDFIHRCLHKGRPVLV
                     HCGFGVSRSAAIGLLYLAAYTDVLPRGSLAEAEEAYRRIYPPYKPGRGIRGFMEAHWD
                     EYTGGGPERDR"
     gene            complement(3175..4578)
                     /locus_tag="PK196_00590"
     CDS             complement(3175..4578)
                     /locus_tag="PK196_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39954.1"
                     /translation="MVPVPELFVAMMKEEWRIHSTIFGNLGFALFPAVIAVFAFLGSL
                     TLPIFADVLPYDVAALLAHIIFLLMGVMVGSFGLMGREVMNRRFGQASLVAYASRSLP
                     VSERRIFAAFLAKDTLYYILLYVLPFSIGFAAATPFISLDPGYALLAFISLTLAFLLG
                     LALVCFLSTLYAHSVALLAGTVAVLAVVGLAASRFGVPLLPPYAFFLDPAPEPLLLSL
                     ILILIPAAVSVLFVTAEYPDRTKRFRNRFKPMAEHLNGLGSARFIAKDALDLIRSEGG
                     VGKVIFSFLLPLALIWVCLQVLIRFIPGIDPLVVFAVLLGVLSATIYNWLTEFDSFTS
                     YAFLPVEVSEVIDAKLKAYALANLLPLAVLVIAAATTEWTVAFFPALAAFVSISAYTL
                     AVTVYLCGLHPNVMLYSAGVFLRYILAISPALLLLIFASILDASYAFAALILIPPVAL
                     LLSRARKKWQTWDMPGT"
     gene            complement(4562..5281)
                     /locus_tag="PK196_00595"
     CDS             complement(4562..5281)
                     /locus_tag="PK196_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON39955.1"
                     /translation="MIEAENLTKDYGNVVAVNSVSLSVDEGELFGLLGPNGSGKTTMI
                     RMLTGQVRPTSGSARVMGIDVTKDPIGVRRLVGIIPEQETPPSFLTAEEYLHFVARIR
                     NLDSIEERCDHWFDLLEFGDKRDVLCKDLSRGTRQKLMFAQAFLHEPPLALIDEPLIN
                     LDPIMQRTVKDFLQRYVRNGGTIFFSTHILEIAEEICDRVGIIHNGRLLHTGRVDEIT
                     ASGGRSLKDFFLELVLGGTGA"
     gene            5495..7696
                     /gene="glgP"
                     /locus_tag="PK196_00600"
     CDS             5495..7696
                     /gene="glgP"
                     /locus_tag="PK196_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HON39956.1"
                     /translation="MKTEIPYDRFAHVPERIFGLVDLAYNLWWSWHSSASVLFKMVNR
                     AEWKMSRHNPVRMLLDTPPRFLERAAATPEYIRRYDITMHRFRDYMAPGTSWFAQHYP
                     AREPLTIAYFSSEFGLHQSLPFYAGGLGVLAGDHIKASSDLGVPTVAAGFMYAEGYLH
                     QHIEANGWQRNVAEVLDRDAAPVLRVLDDDGKQVVVQVPLVDPPIYVAVWKVQVGRVP
                     LYLLDTHIDKNPPENRSISSRLYTSDLEQRLRQELVLGLGGRKVLHTLGAEYSAMHLN
                     EGHSAFALLERLRERLEAGMSFEEAREQVRGTSLFTTHTPVAAAHDVFPEDLMAKYFR
                     GYSKSLGLSWNDFMALGEDPCRPGAGFNMTAFALRMSRFHNAVSRRHGQVAREMWQPL
                     WPDLPVDEVPIDYITNGVHLPTWLNHRIESLFDQYVDPVYPDWRENYDNPIIWEVVDE
                     IPDDDLWREHQWLKMKLFARIRERKRLKWAGHRDEPANLTAEGLMLDTAALTIGFARR
                     FAEYKRADLIFMDPERLERIVNNRWRPVQFIFAGKAHPADEQGKRILQKIYQYSQDPR
                     FGGRIAFVEDYDEQVAHYLVHGVDVWLNTPIPPMEACGTSGMKAGMNGVLNLSVLDGW
                     WVEGYNGWNGWAFESRGDYREDAATIYDLIENEIAPLYYSRTMDDVPHRWVQMMKESM
                     KSIAPQYCALRMLKEYVTRYYPTVCRFAVGPREQMAGLEGVCPPGPAPEKK"
     gene            7866..8828
                     /locus_tag="PK196_00605"
     CDS             7866..8828
                     /locus_tag="PK196_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39957.1"
                     /translation="MEWKDIVPVLAGAIIILVVAFVVKPALLGEPLTAGSEPVVVRNP
                     PVYAPPEPAATPTPYPGDGPAYTRNFRWTAIDGGVHTTAVRIPEALFQEQRETPRLSD
                     YHIWGRYALADADRPILEDLARRIVPPTGNPEEAYLRLMNLVFFVQQIPYDTDDNTAC
                     YIEGTLPPHAIYVKGGVEYPKYPAEMLVDGRGDCEDAAILMGGLLDALGYDTVLLRYS
                     DHMALGIRMNEFNPYYAAYSPRYFEYEGKHYYYVEGTDFEWENVSEYTTAVIGRPYPI
                     GNTRDVPIPSVRSETPEIIPLRYLPLPEEYRIRQARLPAASGGA"
     gene            8865..9431
                     /locus_tag="PK196_00610"
     CDS             8865..9431
                     /locus_tag="PK196_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39958.1"
                     /translation="MKIPKIAPDRKKALLAVGVVIAVMLLLVLGWTLFVNYTEERQVE
                     VFRNHIAASEADLGQVSAKIIEHMSTPTDDLSLAECDAYVREFAAIADYGRAVTAYHR
                     QIIAADTVPEAYFEAQAAYLRALDNLNRAFTFWLSAATAYDMRDYASAEQNLARADEA
                     WREYIAAIGDYEEVLRAAEEGGGEVAPA"
     gene            complement(9428..10102)
                     /locus_tag="PK196_00615"
     CDS             complement(9428..10102)
                     /locus_tag="PK196_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39959.1"
                     /translation="MHRRYNVLLLILLAVATIASGCMTPDEESAEMQPAPLTTATTPG
                     EMQAAGGQLPAPTPPVPTRGEEVPRSLGFVDPATYRIPTPTPMPTMMRPPDDVRVSEK
                     MVEYAVVSCDYPPRLLATEVYHIPFPYWAVEISATPMNEYPWLKIEIRDANDPNRVVE
                     EIRYSKGELLSSIYVDNNTTEDDEARKAETLTIREGYDDYYFIIRSESLKTLDIRILV
                     PEKYLV"
     gene            complement(10372..11619)
                     /locus_tag="PK196_00620"
     CDS             complement(10372..11619)
                     /locus_tag="PK196_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HON39960.1"
                     /translation="MKILVIDPMAAGVAGYMLPAALINLLGDPSALDPLADAIPDLGL
                     CRHFGYSAETLDISGTRATRLVVEVEGERTGELPEVAAAAASAAGLSQAAYSRALAII
                     GDLGQSGHPPVATVFSVVGTLLLLDRAAFFKGAIVATPPVVGCGVIETDYGKIPAPSP
                     ATLDILTRHRFPFTSAPINMEMTTPTGAALLTGLAERALQQYPTMVPVRVGHGSGGRD
                     IPGRPDLLRVVEGESLPAGEERMIILETNLDDTSGEVIGYAIERLFAEGAVDVFVTPA
                     LGKKNRPVSVISVMTTAGEEDNLIRVLMEETGSLGVRVYEFRKVVAARKKETMLIRVG
                     ERERSVRVKTSTIDGRLIAQKPEYEDLAAIAREEGIPLRFVDEEARLRLSERRREGSN
                     LRGGAGGGCPPDRPNAANSARDE"
     gene            complement(11634..13478)
                     /locus_tag="PK196_00625"
     CDS             complement(11634..13478)
                     /locus_tag="PK196_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39961.1"
                     /translation="MDTERERVLLAAAHAATYKPDYMTVDKLEAASRGHGALVIPAFA
                     AANSLAEDIFCNTGAPEMRLVNMTLIDASAPYLPLDAIIAKAVGEAKRGGAEPANAAL
                     IVATLAYFSGSCARAGVPLGNRKLGAIARIHAGADRTSAIALTTNKFTHRVPAFPAYL
                     AIYQALMDKRLTRLDGAVLPPFVAGGGVMGHASLGEDYNIPELARNAAKIGTEAMMRS
                     MEGAGTTPYPLWPALIAAAVTMELVHPDALLGEEFGKFGEIDSAYLAGKGAVEAAKLP
                     EKLHMRGTGEEYDTARIIGDFGLILKDVGGPSVIGSMVVNELFAGFQESAVIGAGFSG
                     GPVNVPLGHLAGDILPALRLLFSNGGDVEATAGAIRDYKMQSFIDPELALCALNTITR
                     KAEVVNRGSVTEACIRASEAVRDRAIYRRAAKVYEMLKAGRSLEEAAAALDEERRALV
                     EKRGSAALSGFTGKEIRFRFTEIAPHARRTDPFTAKYWGFDPKISYDISIDGKDYHIE
                     NLTARVVPEYALQGKGRENPDMGTAIFVGAVLTQEMQYIGHTIINMVVPAAVAAALGT
                     EPADAAKKAAKGAYLTNAIAGGLKVAEEVAKLAREISTGLNEASTETS"
     gene            complement(13650..14141)
                     /locus_tag="PK196_00630"
     CDS             complement(13650..14141)
                     /locus_tag="PK196_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HON39962.1"
                     /translation="MIPLYIYRDNTCDPAKCTAKKLARRGLARIVPAIAKIPRSTILL
                     DPTAERALSPADRNLPSITALDCSWEVLDTGVVASWRNRRALPFLVAANPVNFGRPFR
                     LTTVEAMAAALYILGEKEHAKTILAPFGWGLRFLEVNAEPLEDYAGAKDSAEVVALQA
                     LYM"
     gene            14177..15022
                     /locus_tag="PK196_00635"
     CDS             14177..15022
                     /locus_tag="PK196_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HON39963.1"
                     /translation="MYVLAAPCILDPSCRAHGITTAEDVRSFLRAIERCRRFSIEMVP
                     LPCPETIYLGADREPGSFMERLAANEFSALLDALEAGVRNAIRERGEPPLAIVGVDSS
                     PACGVNTTYRSSEKEPGRGAFIARFPDIRAIDVREFARYRVYLAAPLFSEAERAYNLM
                     LRDLLEAHFFDVYLPQEVGDTSHTRCRDEHRTIFSKHLAALRDADIVVAVIDGADADS
                     GTSWEMGYAYALGKPLVALRTDFRIAGYHERVNLMLEESADVVTAKEDLPRTLGSLLS
                     GAGAP"
     assembly_gap    15087..15186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15594..17303
                     /locus_tag="PK196_00640"
     CDS             15594..17303
                     /locus_tag="PK196_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON39964.1"
                     /translation="MGSTTDPLSLLLAALKEQPRGMSVSDLAAAVGINRNTVSRYLDV
                     LLVAGQVEMKTYGKAKVFYPSQRVPIAAMLNFSSDLVLVLDRDRRIVQANDAICAFAG
                     RERDDVIGKSIETSPLAAFDHPLIRSRITDALNGREAAEELRFLRHSDELFFRVKFLP
                     TVFNDGTPGVTIILENITEARRAEEALRESESLFRSLVENISDLILNVEGDSTFSYVS
                     PKSMEILGYTPEEMLQKTPFDFMPPADAGKVREQFDALFADPRPRVLFEWTMLHRDGS
                     PVILEVSGTPIYDMIGDFTGYRMVCRDVTDRARALKRVAQWKSFLYSVVNNIPSMVFV
                     REIERDMFVFANKAAEIFLGVTREEMAGKRADEIFPQEMATFFADGDRGIAGRSAPVE
                     ARVSLSGGRTLMLKKIPIYNSRGVLKYVLGIAEDITDRVSAENRLVAERDRAKGYLEA
                     AGVMIAVIGANGTIDLVNRKGNEILGYAEGELTGKNWFMTVVPERLRERLQRNFQRLV
                     AGGVEPPHSEKSPVVTRDGRERQVLWHNALLRDTDGRVVAMVSSGEEIAEDPDDQEHA
                     SCA"
     gene            17408..17971
                     /locus_tag="PK196_00645"
     CDS             17408..17971
                     /locus_tag="PK196_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HON39965.1"
                     /translation="MDIISIILIVVVVLVVIGVAAFSISAYNRFFSLKNSAEATIGQV
                     KVAMKKRLDMIEQLLGAVKSYAAFEKETLTGVTGMRARIGSAGPADLSELERESRSVL
                     GRLIAVAENYPDLKTSQTVNDLMGAVKEVENEIARHRYTYNNIAQQYNTMIDTIPSNL
                     IARMMGFTKIEYLEFGEETERVPTIAF"
     gene            17991..19811
                     /locus_tag="PK196_00650"
     CDS             17991..19811
                     /locus_tag="PK196_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HON39966.1"
                     /translation="MTERQQILTLTALTLLIGVMAVAGANALPGFFAGGLEVEHYDAV
                     FYENGTLIERYTYDVSAPGEYRMLFRYWDAPLSFHAIDRPHIEFSGMTSPAGTIGYVR
                     DYRGEVRTVAGTAGSDEVGTIQNLALDNEVGIFNPDYFAPGTYTVEYRYVLRPPVEYD
                     REWAHLNLKLLDEHVPFRDIRVTLPFAGMIEEVYTHPPTLAVERTGEAVVITGNAPEN
                     DAMNIEMILDPAFMEGISCFPTYVSDVRQQAADANRWPPILYGAAGVLHVLGNVLVLL
                     MPFIFLGVYLRYGREKSFTVPEYLSFTPNTALKPWQVNLLFKGDALDFDEDGFYATLL
                     DLHRQKKILVTEKPEGKGVTVRILSTESTDPYEQRVLNFLANLADDHVVDTADLEDLA
                     RTARRSPGYQHRIVQYQQSLAALTRDVDTSFAYRYIKDGRTLILPLAFLGAIPCGLSI
                     LLLLVAPGAAYLLVPAAVLSLIAAIQVGIAALFPSTLFGFWKGESYREKLEWDAFARF
                     LSDLALIRDYSPEDLSMWGEWLVYGTALGLGDKVEAAMKSLNVNLPDAGVPLYSSMPV
                     IFSPILLFSPPSGGGSGVAGAGGGSFGGGGGFGGGGVGGR"
     assembly_gap    20105..20204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20205..20580
                     /locus_tag="PK196_00655"
     CDS             <20205..20580
                     /locus_tag="PK196_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HON39967.1"
                     /translation="GTVVFIDLEGGFYGIVADKGGRYLPLNLNETYRVDGMRLTFVGE
                     VKRDTATIQQWGTPLEIIDIPWACAKCGGNAGVANPAAVWCVEQGHTYEIRKNPDGSE
                     YGVCIFENGTEIDEWEYYRETH"
     gene            complement(20664..21953)
                     /gene="thiC"
                     /locus_tag="PK196_00660"
     CDS             complement(20664..21953)
                     /gene="thiC"
                     /locus_tag="PK196_00660"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HON39968.1"
                     /translation="MVLMHTLVQACLRGVPSEVETIARDEDLAPHAAARTVARGRIVI
                     PANPVRPHRLCAIGEGCRVRVNVNVGTSGVRCDEDLEVEKAKTALREGADTLMDLSTA
                     GDLARIRRRILELDAPVGTVPVYEAVRRAGSAADVDADLLFNVIREHCKQGVDFLTLH
                     CGVNLDALASLKADPRVMGVVSRGGAFHVAMMAATGEENPLYAEYDYLLEILAEHDVV
                     ASLGDGMRPGALVDAGRLAKSTEYLTLGHLAKRALAAGVQRMIEGPGHIPADQIGCNV
                     RMLKELTDSAPLYLLGPLVTDVAPGYDHLVGAIGGVIACMNGADFLCMVSPSEHLALP
                     EIRDIVEGTRAARIAAHVGSLSRAAARTKNREIRMAEARRVLDWEKQFELALIPDEAR
                     RIRERDSGLETCSMCGDLCAVKMMREILPLPEEHLEP"
     gene            21979..22581
                     /locus_tag="PK196_00665"
     CDS             21979..22581
                     /locus_tag="PK196_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON39969.1"
                     /translation="MHVRWITGGMTYANSFVYGNILIDAGVLPMAVEPYATDIDTIVI
                     THAHYDHIAHLPEIAAMCGDATVCIHHADAPGLVDDARSLAIHFGARSPGMVPDVTLA
                     DGDRIGTLLVIHTPGHTPGGISLYDREARLLFSGDTVFTGGSFGRYDFPGGDRRALAA
                     SIERLSGLEVDSLYPGHGEPAQRDGWRHIAAAREALRLYG"
     gene            22574..23029
                     /locus_tag="PK196_00670"
     CDS             22574..23029
                     /locus_tag="PK196_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HON39970.1"
                     /translation="MDKGVYALLIENSCCRVRIGALGVREFAPGYHIYIGSAQGSGGL
                     GRADRHVNLAFSRDRPPRWHIDHLLLDPHFRPVAVITAVTGRDCECDLAKAIGDPSVP
                     GFGCSDCNCPSHLFHRPGDPVPEILAAFRRLDLDARITRIKNAGDKHRV"
     gene            23026..23577
                     /locus_tag="PK196_00675"
     CDS             23026..23577
                     /locus_tag="PK196_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON39971.1"
                     /translation="MTVLGISGSLRKNGNTATLVNTILERVREAGVETEYISLAGMEI
                     RPCTGCETCRETKWCVTEDDDWGFVARRMVECEVLVLGAPTYYYDINGQTKNLIDRTY
                     SLFHERKLSGRRAVAVAVCGDRGCERTLETIEGFLNAHEFSYLGYVCGRGYAAGDVRN
                     DERAMARAREVADTIVRYIRPED"
     gene            complement(23718..24812)
                     /gene="cofH"
                     /locus_tag="PK196_00680"
     CDS             complement(23718..24812)
                     /gene="cofH"
                     /locus_tag="PK196_00680"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HON39972.1"
                     /translation="MTPPLQQVLDDALAGHRLTEEQAVSLLSARGRDVWRIAAAADEL
                     RERKVGDIVTYVRNQNINVTNLCVNACGFCGFSRKPGDPDSFCHDEAVVREKARAARE
                     RGVTEICTVSGLHPAFNADSYVNIYSWMREEAPGVHIHASNPMEVAYAARKSGISSRE
                     VLERMREGGLSTLCGTAAEILVDDVRRVICPDKIDTATWIRIIKEAHEMGIRSTATIM
                     YGSCESEADRARHLEILREIQDETGGFTEFVPLSFVHPNTPLYRAGLARPGATGREDL
                     LLFAVSRLFLDNFDHIQASWVKIGTKMAGMALLAGADDLGGTLFEESISREAGARGTD
                     YLDPAEMRRIAEDIGRTLRRRTTTYTLLEG"
     gene            complement(24971..>25840)
                     /locus_tag="PK196_00685"
     CDS             complement(24971..>25840)
                     /locus_tag="PK196_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HON39973.1"
                     /translation="ADDPGAFRDDLDTVREKARIARERNVTEICILSGVHPDYTLDSY
                     IDLIRCVREEAPGVDIHTASPDEIAWAAERSGVSTREALEHLRDAGLGTLQGTAAEIL
                     VDDVRRVICPNKCDTATWVGVIKEAHEMGIRSTATIMYGSCESEADRARHLGILREIQ
                     DETGGFTELVPLSYLYENTALYRQGLAPAGATGREDLLLFAVARLFLDNFDHIQISWG
                     KVGTKMAELGLLAGGDDLGGTMFCDDVSVEAGGEGADYLDPAEMRRIAEDLGRTLRQR
                     TTTYEIVASTTPD"
     assembly_gap    25841..25940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26015..26683)
                     /locus_tag="PK196_00690"
     CDS             complement(26015..26683)
                     /locus_tag="PK196_00690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HON39974.1"
                     /translation="MAATNWLQRAANAVRAFRDSDHPGISLARDILWVVAVVGGIALL
                     LYLFAGTWPAVVTIESESMVPHMNVGDLVFVVDENRFGGLVTWAEGQMIGYSSFGDYG
                     DVIVYRPNGADSVHPIIHRAMIYLDTEGVIESGLGAYYDEPHGGYITKGDNNPYTDQP
                     NLMISGIGVIEPVKEEWVVGKALAAVPLIGYLPLHLPEFAAVVILIIIIHDLILARRE
                     EKEV"
     gene            26903..28303
                     /locus_tag="PK196_00695"
     CDS             26903..28303
                     /locus_tag="PK196_00695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HON39975.1"
                     /translation="MLANAEIVRRFLETKHQVHPDVVNYIREQNDPSLIDRIAAAAGD
                     ALVVSTEHIPGLKKVRDGTRFLVDPDVEVIVGSAGSSGSVSGHEDAVHYFRNRYNTLS
                     SMIRTRMTAMPVEALLKSPGRYRDEECSIIGMVAEVRTTPKGHRMVEVEDPTGVIRVL
                     FNKGRDESFEEAERILPDDVIGVKGKLSGDGGLLFAEQLVRPDVPINNAPFKSDRPGK
                     AVLISDVHVGSDTFLQDAWNRFADWLQDSDASYLLIAGDLVDGIGIYPGQENELTIKN
                     IYEQYDVFAEMLCDLPSRIRIVAAPGNHDVVRMAEPQPAIPREFAANFPPNCTLVENP
                     CLLNLQGVRVLMYHGRSIDDMIGLIPGASYERPGEIMVEMLKRRLLAAPYGMRTPIAA
                     MKTDRLVIDPLPEILLTGHIHICGVTRYRGVLGVNAGTWQSQTSFQKQMNVHPTPGRA
                     VVVDLQTLQPGVIDFS"
     gene            28395..30419
                     /locus_tag="PK196_00700"
     CDS             28395..30419
                     /locus_tag="PK196_00700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HON39976.1"
                     /translation="MDLVYLAPLGAILALLFAGYSYLSIRREGTGTELMQKIAAAIHT
                     GAMVYLNRQYRAIAVFVVVLAVVLALVINPLTAACFVLGAALSAAAGYIGMFTATAAN
                     VRTTNAARRGVADAFRVSFASGSVMGMVVVGLGLLGLSIAYVVISTVTGLPQAEVLTI
                     VTGFGLGASSIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDVAGMGADLYESYVGSIIAAMVLGVSVAATMFPNVDMMNVILLPLLIATVGIVASI
                     IGSFFVRTNKTESSAIHMAFNKGLLAAIIVTVVATYFLVTTLLGAEYIGVFYATVAGL
                     IAGFAIGLITEYYTSFERKPTQAIAKSTETGAATTIITGFAKGMESTIWPVLIISVAI
                     FVSYQVAGLYGIAIAAVGMLATLGISLSVDAYGPVADNAGGIAEMSHQKPEVREITDT
                     LDAVGNTTAAIGKGFAIGSAALTALALFSAYSQAVGLSVIDVSVPNVFIGVLIGAMLP
                     FLFCSITMMAVGKAAYNIVHEVRRQFREITGLMEGKADPDYATCIAISTRSALREMIA
                     PGVLAVAAPLVVGFVLGKEALGGLLVGSLASGFLLAVTMANAGGAWDNAKKYIEQGHL
                     GGKGSDAHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVALVFAPLILVI"
     gene            30743..32632
                     /gene="acs"
                     /locus_tag="PK196_00705"
     CDS             30743..32632
                     /gene="acs"
                     /locus_tag="PK196_00705"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HON39977.1"
                     /translation="MADSFAVKLEEKKHYPDPRLKASAWVTDYARTYQEFLRDPAGFW
                     EGIARELSWFEPWDRVLEWNYPYARWFVNGKLNITCNCLDRHVSSSRKDKPAIIWHGE
                     SGEKRILTYEDLHREVMRFANGLLSLGIRKGDRVCIYMPLVPEQIVAMLACARIGAVH
                     SVVFGGFGAGALSMRINDAEAKVLITADVGYRRGKTVPLREIALEALAQAPSIERVVV
                     LRRARPAVELDGVREVDYAELMARSAPECPPEVMDAEDPLFILYTSGSTGAPKGIVHT
                     CGGYAVGTYYTTKCVFDIKENDIYWCTADTGWITGHSYIVYGPLAVGTTVVIAEGTPD
                     YPDPGAYWRIVQDLGVTIFYTAPTAIRMFMRYGDEWPKNYDLSSLRVLGSVGEPLNPE
                     AFEWFYHVIGGGRCPIVDTWWQTETGMHMVTTMIGEPMKPGFAGKPIPGAVVDVVNRH
                     GEPVAPGTGGFLVIKEPWPAMLRTVHGNDERYRTYWNTIPGCYTAGDLAVKDEEGYIM
                     VLGRADDLIVVAGHNIGTAEVESALVSHRAVAEAAVIGKPDPLKGNVIKAFVTLCVGV
                     APGDGLTAELARHVRKNLGPIAVPAEIEYVDGLPKTRSGKIMRRVLKARELGMDPGDI
                     STLEE"
     gene            32639..33928
                     /locus_tag="PK196_00710"
     CDS             32639..33928
                     /locus_tag="PK196_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HON39978.1"
                     /translation="MPDTTPAIFGMPAEKGRWGLVALGLIINLCLGSIYAWSVFTAPL
                     AAHFTEVLGRHVTAGEVLLPFSVFLAFFAIAMPLAGKYIERYGPRNVTIVGGILTGFG
                     WLLASTVSSVEALYIVYGVIGGLGVGITYGVPVAVATQWFPDRRGFAVGLTLLGFGFS
                     AFITANIAGALIAAYGVMTTFRIFGIAFIVLLVLSALPLRFPPAGWRPAGWTPPPPGS
                     GSAPACECDRRTMLRTGTFYGLFACYFIGCLAGLTAISIAQPVGTEVAGVSAALATAL
                     VGFFAIFNGLGRPVFGSLTDRITPQKTAMLTFALIAAASLLIWLVPGVPSYIVAFAIL
                     WGCLGGWLAIAPTATASYFGTCDYPRCYGVVFLAYGAGAIAGPQVAGFIRTASGTYLG
                     VFPLVAALAVVGFVIAWLLMRPPAGVPAPEKEKEEGL"
     gene            complement(33925..34395)
                     /locus_tag="PK196_00715"
     CDS             complement(33925..34395)
                     /locus_tag="PK196_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HON39979.1"
                     /translation="MNQWENSNRNLRAVEERIGYTFRNPSLLERALTRLACTLETGLP
                     PEMHMDALATLGDAVINVAVVEAVVAEGVHDKGAITNRKMNLVNMSRLRALAEEIGLA
                     EHVRWGKGEAAQRVWTSGRVLAECLEALIGAVYLDGGMVPAAEVLRQLGLIKRA"
     gene            complement(34392..34880)
                     /locus_tag="PK196_00720"
     CDS             complement(34392..34880)
                     /locus_tag="PK196_00720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON39980.1"
                     /translation="MAEKRVVLHTTMGDITIRLYDDMPVTAGNFEKLVRSGFYDGTVF
                     HRVIDGFMIQGGDPTGTGSGGPGYTIPDEFVKGHSNVRGTVAMANTGRPNTGGSQFFI
                     NLVDNTYLDWDDPRTPSAHPVFGEVVGGMDVVDRIGKVKTDSADRPKVAVRITKAEVV
                     ER"
     gene            35025..35771
                     /locus_tag="PK196_00725"
     CDS             35025..35771
                     /locus_tag="PK196_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HON39981.1"
                     /translation="MYVLWSERLLPVPAEEASRMNGRAPVFIITGGQGQCKTTFLHQV
                     LGLTVGANVRVRGIIAPGHVRDGRRSGFTLVDLATGEHEELCSIDPDPRCEVHGRFYF
                     RPEALAFGRRALAPPDPRSTDLLVVDEVGRFELQGAVWADRLDRLIGHPHPPMIWTVR
                     RRLLHTVVERWNIKNAVVVDVRTASVMATADAVMDAIWEWREAQAFSRATPAAPQADC
                     IPGLSLLVADAAAAGASQNSPIILRRCYSP"
     gene            36429..36626
                     /locus_tag="PK196_00730"
                     /pseudo
     CDS             36429..36626
                     /locus_tag="PK196_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(36684..37025)
                     /locus_tag="PK196_00735"
     CDS             complement(36684..37025)
                     /locus_tag="PK196_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HON39982.1"
                     /translation="MEPITLVTCSGLSSTGKVTARAGMVVLQRFPGSVEECIPAARLQ
                     EPPCGRILALDGCEDACALKKLRETGVVPDLHIVATECGIVKNGMEEPRFDEIEQLAA
                     AVIAALRGGRK"
     gene            complement(37033..37425)
                     /locus_tag="PK196_00740"
     CDS             complement(37033..37425)
                     /locus_tag="PK196_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HON39983.1"
                     /translation="MKKELIIEWKHVGEDLDTTCERCHDTGTALKAVISEITTILEME
                     GVSVRLIETVLPATAVEESNSLLFNGVPIEELLEGVEVISTPCASCACITCEEDTECR
                     ALRYSGEDYEAIPPELIGRAVARALEME"
     gene            complement(37422..38546)
                     /gene="arsB"
                     /locus_tag="PK196_00745"
     CDS             complement(37422..38546)
                     /gene="arsB"
                     /locus_tag="PK196_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HON39984.1"
                     /translation="MMSGRNKIQTENAAGSRLSLLNRYLSLWIVLAMGAGIAISYFFP
                     GVPAAITGFSVGTTSIPIAIGLILMIYPSLAKVRYEDLGRVFRDRKVLGLSIVLNWVV
                     GPVLMFFLAAVFLAGRPEFMYGLILVGLARCIAMVIVWNDLACGDRDYCAAIVGLNSV
                     FQVFFYALYAYVFITVLPPLLGIAAGTAVPITIASVAATVFVFLGIPFIAGFMTRVTL
                     LQLKGHIWYDTRFIPRISPITLFALLFTIVVIFSTQGGNIVALPLDVLRIAIPLAIYF
                     LLMFFVSFWLSWRLRVDYGRATALSFTAASNNFELAIAVAIGIFGIGSGVAFATVIGP
                     LIEVPVLMSLVNAALRLRDRWYGEEATGRCPVDANGGDTA"
     gene            complement(38837..39742)
                     /locus_tag="PK196_00750"
     CDS             complement(38837..39742)
                     /locus_tag="PK196_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HON39985.1"
                     /translation="MDILTPLAVAGQFFVEIAAELILLFIGITFLVGLLQAYIPEERI
                     RRLLGGRRQGVGNIVAAGLGALTPFCSCSTIPILLGLLEVGIPFGACMSFLFASPLLN
                     PVILALLLALVGVVPTVIYAAITFTSAVVIGLILERFGYGRYVRDVVVEGRPKPCDCA
                     SSHGARIQRAFSFAVNLFRQVFPYLILGAGIGAFIYGFVPGDLIIAVAGPENPLAIPL
                     AAVIGIPMYIRAETLIPISAVLLEKGMGIGAVMALIIGGAGASIPEVTLLAAIFERRL
                     VAAFVATILTVAILAGVLFQVLSVI"
     gene            complement(39767..39991)
                     /locus_tag="PK196_00755"
     CDS             complement(39767..39991)
                     /locus_tag="PK196_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39986.1"
                     /translation="MWHRSNSNKIETLGKPARTDVMIMADKSENKKADGIGSRLKKSI
                     FSSGCGCSEGCCGTRIVPKEKKEGSATKEE"
     gene            complement(40090..40500)
                     /locus_tag="PK196_00760"
     CDS             complement(40090..40500)
                     /locus_tag="PK196_00760"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HON39987.1"
                     /translation="MKKRVLFVCTRNSARSQMAEGCLNARYGDRYQGFSAGTRPGRLN
                     PYAIRAMAEIGIDISGHRAKDLGEFDGREMDVLVAVCEGGVCPIFPWAVEEIHRGFPD
                     PSGLTGTDEEIMAGIRRIRDEITSWIDTTFGASR"
     gene            40797..41192
                     /locus_tag="PK196_00765"
     CDS             40797..41192
                     /locus_tag="PK196_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HON39988.1"
                     /translation="MTAKKRECVEDVHIPPEVEESLCRCGGITGLVEQLPRDEALERE
                     SSIHRVLADPLRLKILAMLAIQPLCVCVIKAVLGIADSKLSYHLSVLKKTGLITGEAQ
                     GNWIIYSLTEMGRRVWTRRIRAAGNQEKE"
     gene            complement(41204..41782)
                     /gene="pdxT"
                     /locus_tag="PK196_00770"
     CDS             complement(41204..41782)
                     /gene="pdxT"
                     /locus_tag="PK196_00770"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HON39989.1"
                     /translation="MDVKVGVLALQGDVTEHIAAFREALTERPGSSVVPVRKAADLAG
                     LDALAIPGGESTTISRLIEKNGLYEALREFEGGVFATCAGMVLVAERVDDPRVRPLGL
                     IPMRVERNAFGRQADSREAMLEVKGLEEPFRAVFIRAPVATEAGRGVEVLARIPEGIV
                     AVRHDRHMAFAFHPELGGDLRLHRMFLEGLGV"
     gene            complement(41772..42668)
                     /gene="pdxS"
                     /locus_tag="PK196_00775"
     CDS             complement(41772..42668)
                     /gene="pdxS"
                     /locus_tag="PK196_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HON39990.1"
                     /translation="MKLEELRFGTELIKRGFASMQKGGVIMDVVNAEQAQVAEDAGAV
                     AVMALERVPADIRATGGVARMADPQKIIEIMDAVSIPVMGKVRIGHFVEAQVLEALGV
                     DMIDESEVLTPADEQFHIDKTRFSIPFVCGARDLGEALRRINEGAAMIRTKGEAGTGN
                     VVEAVRHMRAIMGGIRALKGMDTQELVARAREIEAPADLVIECAKLGRLPVVNFSAGG
                     IATPADAALMMQLGADGVFVGSGIFKSSNPEATARAIVEAVNHYDDPKVIAGVSRGLG
                     EAMKGLDVHTLREEEVLQTRGR"
     gene            complement(42672..43592)
                     /gene="cbiB"
                     /locus_tag="PK196_00780"
     CDS             complement(42672..43592)
                     /gene="cbiB"
                     /locus_tag="PK196_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HON39991.1"
                     /translation="MAAAAFVVAAALLVDRLVGDPQSRYHPVALLGRFIGWWGVPARY
                     PRSLQRAAGVAGAFLTAALFAAPFALVQVAAPLWVYLIIAPFLLKATLAWRALEEHTA
                     AVVRALKEGGIDAGRASAGMMVSRDTAHLDQEHVLSAAYESMTENLVDSIVSPLLYFG
                     LFGLTGAAVFRAINTMDAMLGYRDERLRLGWFPARADDIANFIPARLTGLVLLAYFAA
                     KGRFMPALEVLRRDAKNRPGFNGGIPMAVIAGGVGIRFEKPGVYTIGNPERTLEEAGA
                     GIVSAVRAAVILVAILEIATVFLLWFMSYT"
     gene            complement(43571..44095)
                     /locus_tag="PK196_00785"
     CDS             complement(43571..44095)
                     /locus_tag="PK196_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39992.1"
                     /translation="MPEIHLNRRGINSIEVPKEVEVTVGSDLVLDLINHGSPLHITLA
                     STNSSAFTGFFHENLYVNGTEEFHIPIKEDAPPGFFTLQVVTGYGTRRQEFRVVVRGR
                     PEPAPEPRVTPAQAAELAPSRGLHYSLSILILVIAAVVLYGLWVVYRADLLNAAAFVA
                     LLLGVILAWLRRRS"
     gene            complement(44105..44290)
                     /locus_tag="PK196_00790"
     CDS             complement(44105..44290)
                     /locus_tag="PK196_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HON39993.1"
                     /translation="MRKNLMEILCCPVCKSELTLSVTEESGEEVLEGSLRCPACGVDY
                     PISEGIPNLLPQTGCED"
BASE COUNT         8357 a        13431 c        13337 g         8907 t
ORIGIN      
        1 ttccccatag atacggtgcc ctgtaggagg caggctctta tcggtctcac cgaaatctcc
       61 atgaaatccc gtttccttcc cgtgcacggc tatccggact atcgccactt actgcccgca
      121 ccctctggag ggggcgggag gaggccttga tgagcggccg ggcggcgggg gtgtcccatg
      181 aataccgttc aaccagagac gcaggagaca ggggagggag tgggcagtcg taataacggg
      241 aaaagatggg gggtttatat cacagaaccg ttgatttgac ggcctcgatg ggataaacca
      301 ccaaatcaac gtcaatgatc cgggctatag actattcacc attttccgtc agcctgcccg
      361 gagatcacca cccccaccac gcggcgctaa tggatggctg gaacctttaa cctcccggtt
      421 cgagcaatgc acttctgcca tcctgaagat gtattacact gccgttcgag agagtgtggg
      481 ggaatgtgga ctgggggttc acccctcgct ctttcacccg tgactcaaaa tacatccatc
      541 tctcttcaac ccgatctttt gccgggtgga acacaggttg tttatcggtc ccttttaacg
      601 aacgcggcgt aaccgtggta accaccctcg gtggcggttc tgaggacgcg gacgtctgta
      661 gcgccccgcg tctccagttg tctggcgtag gtccatgcgg cctgctgggt ctcgtagagc
      721 ctgtcggcgt agtagtcctg cccgtcgatt acccgccgct gccgtatcct gatccaatcc
      781 gtcttcgagc cccgatccat ggtttagttc ttacaggtga aaggctgata gatgttgcgc
      841 atccggggtc tctctcccga tctaatgttt tgcccctctc tcagcccagt aggcctctgc
      901 ctcttcctcc atctcctcga gtctctcata gtagtccggg aactcgttta agtgcgcaag
      961 cgcgatcttt gctgtcatga ggaggtcgtc atcggtgacg tttgtcaccg gatcccgaag
     1021 cccgtgctcg agttcgacta ccattccccg ccggaactcc tcgagatcga actcatccca
     1081 cgtgatgccg agtttctccc ccatctccct tgcctcatcc agtgttacgt ctcttcgtgc
     1141 cattgggtat ctccatctcg aatggtctct ttgcagggtt aagagcctga cggaaagggt
     1201 atccatccca cccccccgcc gctgccgggc cgtctcgaac gcctcaaatc atattcagcg
     1261 ggccagaagt aaggtttatg ccggttgttc gcatattccg agaagagtag cacactcacc
     1321 gagaggagtt gaatgccccc gtgacgaccg tgattgccgt tgacctcggc ggaaccaacc
     1381 tgcgcgctgc ccttgtggat tccgatgcga atattctcgc ccacgaaaca gttccgacgc
     1441 cgggagccgg gcgttcaggt gagatgatca ccgatgcgat catggcgcgg gttgagactc
     1501 tcctcgcgtt gccggagggg aaggatgctc tggccatagg tgttgcgtcg gtaggcccgc
     1561 tggacctggg tcgcgggtgt gtcataaact cgccgaacat cgccttccct ctcgtcgaga
     1621 tcgtcgagcc gctgaggaag cggttcgggc ggcctgtctt cctcatcaac gatgcccggg
     1681 ccggtgtcct gggcgaacgc tggatcggtg ccgcccgcgg ttccgagaac gctgtctaca
     1741 tcacactctc gaccggcata ggtggcggtg ctgtggtgaa cggctctctc ctcctcggga
     1801 aggacgggaa cgccggcgag atcgggcaca tacccgttga tacacgctgc aacctcatct
     1861 gcgggtgcgg gtttgccggc cactgggagg cttacgcctc cgcaaagaac atccccacct
     1921 tctttgcggc ctggcaggag gcggcgggtc tccggcaggt gagtttcgac acctgctcga
     1981 cgcaagcgat cttcgaggcg gccagggagg aggacgcggt tgcccttgcc ttcgtggagg
     2041 cgctcgggaa ggtaaacgcc cgtggggtct caggggttat cgtagcctac aaccctgaga
     2101 taatcgtgtt tgacggcccg cttgcgcgct atcacggcga tatcgttatc aggtacatgg
     2161 agccgttcat cgaccgctac ctgacccttc cccggctggc agtctcctca ctcgacggta
     2221 aagcaccgct ctttggtgcg gcgttatacg cgcttgaggc cctctgagag tccgggtgag
     2281 aagaatgatc gagatctacc ccaatctata catcgggtcg caggaggatt atgaggttgt
     2341 ggtggaggcc catgatacgt ggtgcgtggt gcatgcctgc cggaatccat accactgcct
     2401 ggcggtcaca tactccccgc tcggcacggt gccggcagac cacccggaac acctggttgc
     2461 ccggcgggga aaccgcctta tgctcaacct ggtcgactcc cgggatcccg ccgatatacc
     2521 gaaagaggcg atcgatgccg cgctggattt tatacaccgt tgccttcata aagggcggcc
     2581 ggtgctggtc cactgcgggt tcggggtatc ccgctcggcg gcgatcgggc tcctctacct
     2641 ggcggcatac accgatgtcc tgccccgcgg gagcctggcc gaggccgagg aggcatatcg
     2701 gaggatatac ccgccatata agcccggtcg cgggatcagg gggtttatgg aggcgcactg
     2761 ggatgagtat acaggagggg ggccagaacg cgaccgctga accgccgcgc accgccgtga
     2821 tcagggtccg gcggtctcga ctctacgccc acctctatcc cgtcgaaggg ctggatgacc
     2881 ctggtcccat tcttgcgggt catcgggatg cataccggaa gcccgcccac cactgcgggt
     2941 gtgtgggggt gttgaggtgt tcaagataac ggtggcgagg tcggtcgacc cggacatgtg
     3001 ctcctcgctc tcctccgaaa gcatggtctt gatagccagg cgctggtgag atccaggatc
     3061 ttttggggtg tttcgcacgt gcgatccgcg atgccggggt cagctggtga cgccctcaag
     3121 gaggataacc cacccctgag atcggcacat ccaccgtgcc cggctgaaaa actatcaggt
     3181 tcccggcata tcccatgtct gccacttctt ccgcgctctg gagaggagga gcgccaccgg
     3241 cgggatgagg atgagggctg caaacgcgta tgatgcgtcg aggatcgagg cgaagatcag
     3301 gagcagaagt gccgggctta tagcgagtat gtatcgaaga aagacccccg cagagtagag
     3361 catcacgttc gggtggagtc cacagaggta gaccgtcacc gccagggtgt acgccgagat
     3421 cgagacgaag gcggcaaggg ccgggaagaa cgccaccgtc cattccgttg tcgccgctgc
     3481 tatcacgagc accgcgagcg ggagcaggtt tgcaagggcg taggccttca gtttcgcatc
     3541 gatcacctcg ctgacctcca ccggcaggaa ggcgtaggac gtgaaagagt caaactcggt
     3601 cagccagttg tatattgtgg ccgataacac cccgagcagg acggcaaaga ccaccagggg
     3661 atcgatcccc ggaatgaacc ggatcaggac ctggaggcag acccagatca gggcgagcgg
     3721 gaggaggaac gagaagatca ccttcccgac gcccccctcg ctcctgatga ggtcgagggc
     3781 gtcctttgcg atgaagcgcg cgcttcccag gccgtttagg tgttcggcca tgggcttgaa
     3841 ccggtttctg aaacgttttg tccggtcagg atattccgca gtgacaaaga gcaccgatac
     3901 tgctgccgga atcaggatga ggatcagaga taggaggagc ggttccggcg ccgggtcgag
     3961 gaagaaggcg tatggcggga ggaggggcac cccgaaccgc gaggccgcca acccgacgac
     4021 ggcgagcacc gccaccgttc cggccaggag cgcaacagag tgtgcgtaga gcgtcgagag
     4081 gaagcagacg agagcaagac cgaggaggaa ggcgagcgtg agcgatataa atgccaggag
     4141 agcgtagccc gggtctagcg agatgaacgg tgtcgctgcg gcgaacccga tcgagaacgg
     4201 aaggacatag agcaggatgt agtataacgt gtcttttgcc aggaaagcgg cgaatatccg
     4261 tctctccgag accggcaggc tccgggacgc gtaggcgacc agactggcct gcccgaacct
     4321 gcggttcatg acctcccggc ccatgaggcc aaacgaaccc accatcacgc ccatgagcag
     4381 gaagatgatg tgtgcaagga gcgcggcaac gtcatatggg agaacgtctg caaagatcgg
     4441 gagggtgagc gatcccagga atgcaaagac cgcaatcacc gccggaaaga gcgcaaaccc
     4501 caggttcccg aatatcgtcg agtggatccg ccactcttcc ttcatcatcg cgacaaagag
     4561 ttcaggcacc ggtaccaccc agaaccagtt cgaggaagaa atctttcagg ctcctgccgc
     4621 ccgatgccgt gatttcgtcg acacgaccgg tgtggaggag cctgccgttg tgtatgatcc
     4681 cgacccggtc acagatctct tcggcgatct cgaggatgtg ggtggagaag aagatcgtcc
     4741 cgccgttcct gacgtaacgc tgcaggaaat cctttaccgt cctctgcatg atcgggtcaa
     4801 ggttgatcag aggttcgtcg atgagggcga gcggaggttc gtggaggaag gcctgcgcaa
     4861 acatcagttt ctgcctggtt ccccgggata ggtccttgca caggacgtcc cgcttgtccc
     4921 cgaactccag caggtcaaac cagtgatcac acctctcctc gatcgaatcc aggttgcgga
     4981 tgcgggccac aaaatggagg tactcttctg cggtgaggaa acttggcggt gtctcctgct
     5041 ccgggatgat cccaaccagt ctcctgaccc cgatcgggtc tttcgtcaca tctatcccca
     5101 taaccctggc cgaaccggac gtcggcctta cctgccctgt cagcatccgg atcatcgttg
     5161 tcttccccga gccgttcggc ccgagaagcc cgaagagttc cccttcatca acggagagcg
     5221 agacactgtt cacagcgacg acgttgccgt aatccttggt gaggttctct gcctcgatca
     5281 tatgccgcac gcccaatttg tgcaccacct gaccattcag ttaaagagtg tcggctggag
     5341 gtatctataa tcacttcccg tacccttacc atctgatggg gccactcgtg ccaggcatgt
     5401 cataagcaga gggtttttac aggctcttta cccgtgcgcg ccggcccctc ccgacagaag
     5461 accttatgtg gtgcaacacg caatgcctca cgagatgaag acggagatac cctatgaccg
     5521 gttcgctcac gtcccggagc ggatcttcgg tcttgtcgac ctcgcttaca acctctggtg
     5581 gagctggcac tcctcggcaa gtgtgctctt caagatggtg aaccgggcgg aatggaagat
     5641 gagccgtcac aaccccgtca ggatgctcct ggacaccccg cctagattct tagaacgggc
     5701 ggcggcgacc cctgaataca tccgccgcta cgatatcacc atgcaccggt ttagggatta
     5761 catggcgccg gggacaagct ggtttgcgca gcactacccg gcgcgggagc cactcacgat
     5821 cgcctacttc tcaagcgagt tcggactgca tcagtcgctc ccgttctatg cgggagggct
     5881 cggcgtcctg gcgggggacc acataaaggc gtcgagcgac cttggtgtcc cgacggtcgc
     5941 cgcagggttc atgtacgccg aagggtatct ccaccagcac atcgaggcga acggctggca
     6001 gaggaacgtc gcggaggtcc ttgaccgtga tgctgccccg gttctccggg tgcttgacga
     6061 cgacggcaag caggttgtgg tgcaggttcc cctggttgat ccgccgatct acgtcgccgt
     6121 ctggaaggta caggtagggc gggtccctct ctacctcctc gacacccata tcgataagaa
     6181 cccgcctgag aaccgtagca tatcatccag gctttatacc agcgatctgg agcaacgcct
     6241 ccggcaggaa ctggtgctcg gactcggtgg gcgcaaggtt cttcacacgc ttggcgcgga
     6301 gtactcggcg atgcacttaa acgaggggca ctccgcgttc gccctcctcg aacggctccg
     6361 ggagcggctc gaagcgggga tgtcgtttga agaggcgcgt gagcaggtcc ggggcacttc
     6421 cctcttcaca acccacaccc ctgtcgccgc tgcccacgat gtctttccag aagatctgat
     6481 ggcgaagtat ttccgcggtt attctaagtc gctcggacta tcctggaacg atttcatggc
     6541 gttaggggag gatccgtgcc gtccgggagc aggtttcaac atgacggcgt ttgctctccg
     6601 gatgagccgg ttccacaacg ccgtctccag gcgtcacggc caggttgcaa gggagatgtg
     6661 gcagcctctc tggccggatc tgccggtaga tgaagtgccg atcgactata tcacgaacgg
     6721 cgttcacctc ccgacctggc tgaaccaccg gatcgaatcg ctatttgatc agtatgtgga
     6781 tccagtctac cctgactggc gggagaatta cgacaacccg atcatctggg aggtggtaga
     6841 cgagatcccg gatgatgacc tctggcgtga gcaccagtgg ctgaagatga aactctttgc
     6901 ccgtataaga gagcggaaac gcctcaagtg ggcgggccat cgcgacgaac ccgcgaacct
     6961 cacggccgag gggctcatgc tcgacaccgc agcgctgacg atcgggttcg ctcgccggtt
     7021 cgccgagtac aaacgtgcgg atctcatctt catggatcct gagcggcttg agcggatcgt
     7081 gaacaatcgc tggaggccgg tgcagttcat ctttgccggg aaggcccacc ctgccgacga
     7141 gcagggcaaa cggatcctcc agaagatcta ccagtattcc caggatccac ggttcggcgg
     7201 caggatagcg ttcgtcgagg attacgacga gcaggttgca cattacctgg ttcacggtgt
     7261 agacgtctgg ttgaacacac ccattccacc catggaggcg tgcggcacga gcggtatgaa
     7321 ggcggggatg aacggcgtgc tgaacctctc ggttctggat ggctggtggg ttgagggcta
     7381 caacggctgg aacggctggg cttttgagag tcggggggat tacagggagg atgcggcgac
     7441 gatctatgac ctgatcgaga acgagatcgc cccgctctac tactcgcgga cgatggacga
     7501 cgtgccgcac aggtgggtcc agatgatgaa ggagtcgatg aagagcatcg ccccacagta
     7561 ctgcgccctc cggatgctca aggagtacgt caccaggtac taccccaccg tctgcaggtt
     7621 tgcggtcggg ccgcgggagc agatggcagg gcttgaaggg gtatgtccgc cgggtccggc
     7681 acctgaaaag aagtgatctg gagtcggcaa agcacagcgg cggggggtag cgtctccgcc
     7741 accagatcta caacagcatc gaaccactct gatggtcaat gcctcggccc tgccggggtt
     7801 aacgttttct acaccccggc tataaacaat cactaccgga gggccgcctg tccggggtct
     7861 tggatatgga gtggaaggac attgtcccgg ttcttgcggg tgcgatcata atcctggtcg
     7921 tcgcgtttgt agtgaaaccc gcgctactcg gcgaaccgct caccgctggt tccgaaccgg
     7981 tggtggttcg aaacccgccg gtctatgccc ctcctgaacc ggcagcgaca ccgacaccgt
     8041 atccgggtga cggtccggct tacacgcgga attttaggtg gacggcgatc gatggcggag
     8101 tccatacgac ggcggtcagg atcccggagg cgctgttcca ggaacagcgg gagacgccgc
     8161 ggctcagtga ttatcacatc tgggggaggt atgccctggc cgacgctgac aggccgatcc
     8221 tggaggacct tgcccggcgt atcgtgccac cgaccggaaa tccggaggag gcctacctac
     8281 ggctgatgaa ccttgtcttc tttgtccagc agatccccta cgatacggac gacaacacgg
     8341 cctgctacat cgagggcacc ctcccgccgc atgccatata cgtaaagggc ggtgttgagt
     8401 atcccaaata tcccgccgag atgcttgttg atgggagagg ggactgcgag gacgcggcca
     8461 tactgatggg agggctcctc gacgccctgg gctacgatac ggttctgctg agatactccg
     8521 atcatatggc cctcggcatc cggatgaacg agttcaaccc ctactacgcg gcttactcgc
     8581 cgaggtactt tgagtatgag gggaaacact actactacgt tgaggggacg gattttgagt
     8641 gggagaatgt ctcggaatac accacggcgg tcatcgggag accctatccg atcggtaata
     8701 cccgggatgt ccctatcccg agcgtgagat cggagactcc cgagatcatc ccgctccgct
     8761 atctccccct gccggaggag taccgcatcc gccaggcccg gctaccggcg gcatcagggg
     8821 gagcgtgagg cagaatggac ctggctctac ctgcgccccc ccgcatgaag atcccgaaga
     8881 tcgcccctga ccggaagaag gcgctccttg ctgtcggggt tgtgattgcc gtgatgctgc
     8941 tgctggtttt aggctggacg ctgtttgtga attacaccga agagaggcag gtcgaggtgt
     9001 tccgcaacca catcgcggcg tctgaggccg atctcggcca ggtctctgcg aagatcatcg
     9061 agcacatgag cacccctaca gacgatctct cgcttgccga atgcgatgcc tacgtgcggg
     9121 agtttgcggc catcgcggac tacgggaggg cggtgacagc ctaccaccgc cagatcatcg
     9181 cggccgatac cgtcccggaa gcctatttcg aggcgcaggc cgcctacctg cgggcgctcg
     9241 acaacctcaa ccgtgcgttc accttctggc tatctgcggc gaccgcctac gatatgcgcg
     9301 attacgcctc cgcagaacag aacctcgccc gtgccgatga ggcgtggcgg gagtacattg
     9361 ccgcgatcgg ggactacgag gaagtactcc gtgcagcaga ggaaggagga ggagaggtcg
     9421 ctcccgccta gaccaggtac ttctcgggga cgaggatcct gatatcaagg gtcttcagcg
     9481 actccgagcg gattatgaag tagtagtcgt catacccttc ccggatggtt agtgtctctg
     9541 cctttcttgc ttcatcatcc tccgtggtat tgttatcaac gtagatgctc gagagaagtt
     9601 cgccctttga gtagcggatc tcctcaacca ccctgtttgg atcgtttgca tcccttatct
     9661 caattttgag ccaggggtat tcgttcatcg gcgttgcgct gatctcgacc gcccagtagg
     9721 ggaaggggat gtggtagacc tcggtggcga gaagtctggg tgggtaatcg catgatacca
     9781 ccgcatactc aaccatcttc tcggagacac ggacatcgtc tggcggtctc atcatcgttg
     9841 gcatgggtgt cggggttggg atgcggtacg tcgcggggtc aacgaatccc aggctccgcg
     9901 ggacctcctc cccgcgggtg ggcacgggag gtgttggtgc cgggagttgc cctccagctg
     9961 cctgcatctc acctggggta gtagcggttg tgagtggggc gggttgcatc tccgcgctct
    10021 cctcatccgg agtcatgcac ccgctggcga tggtggcgac tgccaggagg atcaggagca
    10081 ggacgttgta gcgccgatgc atacagttct cttgttttgt gcacactata taaaagatgc
    10141 atcgtgggga tctgcataga tcatcaagct cccacgggca ggtggttatg cgaagatcct
    10201 ttgaggccgg atgcagcgga ttgatgtgga aaagggtgtt tgattgggaa tatgaaatag
    10261 gatcgtataa atatcgcgtt gatcatctaa ttacgtgggg aactaatacc ccggtgacat
    10321 gagagaactg tagatgcgag tggcgcacgt cccgggggga cgtgccggta ttcactcgtc
    10381 gcgtgcggag ttcgccgcgt ttgggcggtc gggtgggcat ccaccccctg caccccctct
    10441 gaggttggag ccctctcttc ttcgttccga taagcgcagt ctggcctctt catccacaaa
    10501 tcggagaggg atcccctctt cgcgtgcgat tgccgcaaga tcttcgtatt cgggtttctg
    10561 ggcgataagg cggccatcta tggtcgaggt ctttacccgg acggaacgct ccctttcacc
    10621 gacccggatt agcattgtct ccttcttacg ggcggccaca accttccgga actcatacac
    10681 ccgcaccccc agactaccgg tctcctccat tagaaccctg atcaggttat cctcctcccc
    10741 tgccgttgtc atcacagaaa tgacactgac gggccggttc ttcttcccga gcgccggggt
    10801 gacaaagaca tccaccgccc cttccgcaaa gaggcgctcg atcgcgtagc cgatcacctc
    10861 ccccgaggtg tcgtcgaggt tggtctcaag gatgatcatg cgctcctccc cggcggggag
    10921 gctctctccc tccacaaccc tgagcaggtc gggccgtcca ggaatatcgc ggccgccgct
    10981 gccgtggccg accctgacgg gaaccatggt cgggtattgc tgtagcgcgc gctccgcaag
    11041 accggtgagc agcgccgccc ctgttggggt tgtcatctcc atattgatgg gggcggaggt
    11101 gaaggggaac cggtgccgtg tcaggatatc gagggtggca ggcgaaggcg ccgggatctt
    11161 gccgtaatct gtttcgatca ccccgcaacc aactaccggc ggcgtcgcga ctattgctcc
    11221 cttgaaaaac gccgctctat cgaggaggag cagcgtcccg acgacggaaa agaccgtcgc
    11281 caccggcgga tgcccggact gaccgaggtc gccaatgatc gcgagcgctc tggaataagc
    11341 ggcctgtgag aggcctgctg cactggcggc agcggccgcc acctccggga gttctcctgt
    11401 tctctcccct tcaacctcga ccacgagccg ggttgctctg gtaccgctga tatccagtgt
    11461 ctcggcgctg taaccaaagt ggcggcagag ccccagatca gggatggcat ccgcaagcgg
    11521 gtcgagagcc gatgggtcac caagcaggtt gatgagggcc gcagggagca tataccctgc
    11581 aacccctgca gccatcgggt cgatgacgag gattttcatg ccccgccgat ccttcaagat
    11641 gtttcggttg atgcttcgtt gaggcctgta gagatctccc gtgcgagttt agcgacctct
    11701 tctgccactt tcaggcctcc ggcgatggcg ttcgtcaggt atgcgccctt tgcagccttc
    11761 ttcgccgcat ccgcaggctc ggtcccgagc gctgccgcta ccgcggcggg aacgaccatg
    11821 ttgatgatgg tgtgcccgat gtactgcatc tcctgtgtca ggacagcgcc aacgaagatg
    11881 gcagtgccca tatcggggtt ctcccttcct ttcccctgga gcgcatactc cgggaccacc
    11941 ctggcggtga ggttctcgat atggtagtct ttcccgtcga tacttatgtc ataagagatc
    12001 ttcgggtcaa aaccccagta ttttgcagta aaggggtctg ttctccgtgc atggggcgcg
    12061 atttccgtga accggaacct gatctccttc cccgtgaacc ctgagagggc tgcggagcca
    12121 cgtttctcaa cgagagccct gcgctcctcg tcgagggctg ctgccgcctc ctccagcgac
    12181 cttcccgctt tgagcatttc gtagaccttc gccgcccgcc ggtagatcgc acggtcacgc
    12241 acagcctcgc tcgcgcgaat gcaggcctcc gttaccgatc ccctgttcac gacctccgcc
    12301 ttgcgggtga tcgtgtttaa ggcacagagg gcgagttcgg ggtcgatgaa ggactgcatc
    12361 ttgtagtccc ggatcgcccc ggcggttgcc tcgacatcgc cgccgttcga aaagagcaac
    12421 cgcagcgccg gaaggatgtc gccggcgagg tggccaagcg ggacgtttac cgggccgccg
    12481 gagaacccgg cgcctatgac ggcggactcc tggaaccctg cgaagagttc gttcaccacc
    12541 atcgacccga tcaccgaggg gccgccgacg tcttttaaga tcagcccgaa atcgccgatg
    12601 atccgggcgg tgtcatactc ttctccggtt ccgcgcatgt ggagcttctc cgggagtttc
    12661 gccgcttcga cggccccttt tccggccagg tatgcagagt caatctcccc gaacttcccg
    12721 aactcctcac ccaggagggc gtccgggtgg acaagttcca tcgtcaccgc cgcggcgatc
    12781 agcgctggcc agagggggta tggcgttgtt ccggcaccct ccatgctccg catcatcgct
    12841 tcggtcccga tcttcgcggc gtttctggcg agttccggga tgttgtagtc ctcgccgagc
    12901 gaggcgtggc ccatgacacc cccgccggcg acgaacggcg gcaggacagc gccgtcgagg
    12961 cgggtaaggc gtttatccat cagggcctgg tagatcgcga ggtatgcggg aaacgcgggg
    13021 acgcggtgag tgaacttgtt cgtcgtcagg gcgattgcgc tcgtcctgtc tgcaccagca
    13081 tgtatcctgg caatggcgcc aagtttccgg ttcccgagcg gcacgcctgc ccgggcgcag
    13141 ctcccggaga agtaggcgag cgttgccaca atcagagccg cgtttgccgg ttctgccccg
    13201 ccccgcttcg cctcgccaac cgctttagcg atgatcgcat cgagcggcag gtagggggcg
    13261 gaggcatcga tcagcgtcat gttcacgagg cgcatctcag gcgcgccggt attgcagaag
    13321 atatcctctg ccagtgagtt tgccgcggca aatgccggga ttacaagcgc cccgtgcccc
    13381 cgactcgccg cctccagctt gtcgacggtc atgtagtcag gcttataggt tgcagcatgg
    13441 gctgcagcca ggagaaccct ctctctttcg gtatccatct tctgataacc tccttgtctc
    13501 tgtctgcgag atcatggttg aatatataaa aagatgctgt ttggggaaga cggcgacgtt
    13561 tcaaattgca cccctgtttc cggttgagcg gatgaaaggt tttagcgatg tatgaggtgt
    13621 tgactcatcc gaaggccgca ggaggcatct cacatgtaga gcgcctggag ggcgaccacc
    13681 tcggcgctgt cctttgctcc tgcgtagtct tccaggggtt cggcgttcac ctcaaggaac
    13741 cggagccccc acccaaacgg cgcaagaatg gtttttgcat gttccttctc ccccaggatg
    13801 tagagcgctg ctgccatcgc ttccaccgtt gtcagcctga acggtcggcc gaagttcacc
    13861 gggtttgcgg ccaccaggaa cggcagtgcc cggcggttgc gccacgaggc gacgactcct
    13921 gtatcgagaa cctcccagga gcagtctagc gctgttatcg aggggaggtt acggtcggcg
    13981 ggggagagcg ccctctcggc ggtagggtcg aggaggatgg tcgaacgcgg gatctttgcg
    14041 attgccggga cgatacgggc aagtccgcgc cttgcgagtt tctttgccgt gcacttcgcc
    14101 gggtcgcagg tattgtcccg gtagatgtag agcggtatca tctgtcttgt tatggtaggc
    14161 cgcccaggta gtatcaatgt acgtgcttgc cgctccctgc atcctcgatc cctcctgccg
    14221 tgcccacggg ataacgaccg cggaggacgt tcgctccttc ctccgtgcca tcgagcgctg
    14281 ccggcgtttc tcgatcgaga tggtcccgct cccctgcccg gagacgatct accttggcgc
    14341 cgaccgggaa ccgggctctt tcatggagcg gctcgcagcc aatgagtttt cagcgctcct
    14401 ggatgcgctt gaggctggtg ttcgtaacgc gatcagggag cggggtgagc cgccgctcgc
    14461 gatagtgggg gtggactcct ccccggcctg cggggtcaat acgacctacc gctcctccga
    14521 aaaagagcca gggcgcggag cgtttattgc caggttcccg gatatccggg cgatcgacgt
    14581 cagggagttt gcgcgctacc gcgtctacct tgccgcgccg ctcttctcgg aagcggagcg
    14641 ggcctacaac ctgatgctcc gtgatctcct cgaggcgcac ttcttcgatg tctacctccc
    14701 ccaggaggtg ggggatacca gccacacccg gtgccgggac gagcacagga ccatattctc
    14761 aaagcatctt gcggcgctcc gcgacgccga catagtcgtt gcggtcatcg acggtgcgga
    14821 tgcagactca ggcacatcct gggagatggg gtatgcttac gctcttggca aacctctcgt
    14881 cgcgctccgc accgactttc gtatcgctgg ataccacgaa cgtgtcaacc tcatgctcga
    14941 agagtccgca gacgtggtaa cggcaaaaga ggaccttccc cggacgctgg ggtcacttct
    15001 ctcaggcgca ggcgcaccat gagtcatggg caaaaaggaa tatttgaggg tgtgtgataa
    15061 ccatgcgatg cctgcacgtg gcccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnaaaa aggaatattt gagggtgtgt gataaccatg cgatgcctgc acgtggcccg
    15241 gagacggttg tgccgcccgc cccggcgccg cagggcatcc atcggaggta gtgttcagag
    15301 aggtgtgccc tgatgtacct gaaagccagg tacacccgat ttttgataca gtcagcgtgg
    15361 tgaaggtttc cgttcaccgt tcccggccag accggatcac ctgtgcccac atgatccggt
    15421 gcctgtcgga gagattcccc tttttccgac cccggggatc atgagttgat caggttaagt
    15481 aataaagttt ttgttcattg ccatgcatta tggtcttgcg ttgtatactg gcaccgttga
    15541 atggcaaaaa gcataaagta ctataactcc ggccgataaa cactcctctg cgcatgggct
    15601 ccaccaccga tccactctct ctcctgcttg ctgctctcaa agagcaacct cgcgggatgt
    15661 ccgtctcaga cctcgcagca gctgtgggga tcaaccggaa caccgtctcc cgctacctcg
    15721 acgtcctcct ggtggccggg caggtggaga tgaagacata cggtaaggcc aaggtttttt
    15781 atccctcgca gcgggttccc atagccgcca tgctcaactt ctcctccgat ctcgtgctgg
    15841 tgctcgaccg ggatcgcagg attgtccagg caaacgatgc catatgtgca tttgccggga
    15901 gagaacggga tgatgtcata gggaaatcca ttgagacgtc accgcttgcc gcgtttgacc
    15961 acccgctgat acggagccgg atcaccgacg ccctgaacgg ccgtgaggcc gcggaggagc
    16021 ttcgcttcct ccggcacagc gatgaactct tcttccgggt caagttcctg cccacagtct
    16081 tcaacgacgg cacgcccggt gtcaccatca tccttgagaa catcaccgag gcgcggcgtg
    16141 ccgaggaagc gctgcgcgag agcgagtcgc tcttccggag cctggtcgag aacatcagcg
    16201 acctgatact gaacgttgaa ggggactcca ccttctctta cgtcagccca aaaagcatgg
    16261 agatccttgg ctacaccccg gaagagatgc tccagaagac gccattcgat ttcatgcccc
    16321 cggcagatgc cggaaaagta agagagcagt ttgatgctct cttcgccgat ccgaggccgc
    16381 gtgtcctctt tgagtggacg atgctccatc gtgacgggag ccccgtaatt ctcgaggtga
    16441 gcgggacacc gatctacgat atgatcgggg acttcaccgg ttaccggatg gtctgtcgcg
    16501 acgtaaccga ccgggcgcgt gccttaaaaa gggtagcgca gtggaagtcg ttcctctact
    16561 ccgtggtcaa caacatcccg agcatggtct ttgtccggga gatcgagagg gacatgttcg
    16621 tcttcgcgaa caaagccgcc gagatctttc ttggggttac acgggaggag atggccggaa
    16681 aacgggcgga tgagatcttt ccgcaggaga tggcaacgtt cttcgccgac ggtgaccgcg
    16741 gtatcgccgg gcgttctgcg cccgtggagg cgagggtctc tctctccggt ggccggacac
    16801 tcatgctgaa gaagatcccg atctacaact cgcggggtgt ccttaaatac gttctcggga
    16861 tcgccgagga tatcaccgat cgcgtatccg ccgagaaccg ccttgtcgcc gagcgcgacc
    16921 gggccaaggg ctaccttgag gctgcgggtg ttatgatcgc ggtgatcggg gcgaatggca
    16981 caatcgatct agtcaaccgg aagggaaacg agatcctggg ctatgccgag ggggaactca
    17041 caggtaagaa ctggtttatg acggtcgttc cggagcgtct gcgcgagagg ctccagcgga
    17101 actttcagcg gctggtggcc gggggggtag agccgcccca ttctgagaag agcccggtag
    17161 tcacccgcga tggccgggaa aggcaggtcc tctggcacaa cgccctcctc cgtgacacgg
    17221 atggcagggt cgtggcgatg gtgagttccg gcgaggagat cgcagaagac ccggatgacc
    17281 aggagcatgc ttcatgcgcc tgaccctgcc ccatctccca cgcaaaggcc gtctactgcg
    17341 tatttgtgcg atattaatgg ttaggtctta atacgctgca gggcacacag tttattggtg
    17401 gcaaagtttg gatatcatct ccataatcct cattgtcgtg gtggtgctgg tcgtcatcgg
    17461 tgtggcggca ttctcgatca gcgcctacaa ccggttcttc tccttgaaaa attctgctga
    17521 ggcgacgata gggcaggtaa aggtcgcgat gaagaagcgg cttgacatga tcgagcagct
    17581 cctcggcgct gtaaagagtt atgcggcgtt cgagaaggag accctgaccg gggtgaccgg
    17641 gatgcgggcg cgtatcggca gcgccggacc tgcggatttg agcgaacttg agcgggagtc
    17701 tcgctcggtg ctgggacggc ttatcgccgt cgcggagaac tatcctgacc ttaagacctc
    17761 gcagacggtg aatgacctta tgggcgcggt taaagaggtc gagaacgaga tcgcgcggca
    17821 ccgttacacc tacaacaaca tcgcccagca gtacaacaca atgatcgata cgatcccctc
    17881 gaaccttatc gctaggatga tgggcttcac gaagattgag tacctggagt ttggcgagga
    17941 gaccgaaagg gttccgacga tagcgttctg atccggataa gaccatgcgg atgaccgaac
    18001 gacagcagat actcaccctg accgccctca ccctgctcat aggggtcatg gcggttgccg
    18061 gagcaaacgc cctccccgga ttctttgcgg gcggcctcga ggtcgagcac tacgatgctg
    18121 tcttctatga gaacggaaca ctaatcgagc gctacaccta cgatgtcagc gcgccggggg
    18181 agtaccggat gctcttccgc tactgggatg caccgctctc ctttcatgcc atcgaccggc
    18241 cgcatatcga attttccggg atgacctccc ctgccggaac catcgggtat gtcagggatt
    18301 acaggggcga ggtaaggact gtcgcaggga cggccggttc tgatgaggtc gggacgatcc
    18361 agaaccttgc actcgataac gaggtcggca tctttaaccc ggattacttc gcccccggca
    18421 cctacacagt ggagtatcgt tacgttctcc ggccgccggt cgaatatgac agggaatggg
    18481 ctcacctgaa cctgaaactt ctggatgagc atgtcccatt ccgggatatc cgggtcacgc
    18541 tgccgtttgc cggtatgatc gaggaggtct acacccatcc cccgaccctc gcggtcgagc
    18601 ggacgggaga ggctgtggtc ataacgggaa acgctccaga gaacgatgcc atgaacatcg
    18661 agatgatact cgatccggct ttcatggagg ggatcagctg tttcccgaca tatgtcagcg
    18721 acgtccggca acaggcggcc gacgccaacc gatggccgcc gatactgtat ggtgcggcgg
    18781 gtgtcctcca cgttcttgga aacgttctcg tgctccttat gccgttcatc ttccttggtg
    18841 tctacctccg ctacggcagg gagaagtcct tcactgtccc tgaatacctc agtttcacgc
    18901 cgaacaccgc gctaaagccc tggcaggtga acctcctgtt caagggggac gcacttgatt
    18961 tcgatgagga cgggttctac gcgacgctcc ttgaccttca ccggcagaag aagatcctgg
    19021 tgaccgaaaa acctgagggc aaaggggtta cggtccggat actctcgact gagtccaccg
    19081 acccatacga gcagcgggtt ttaaacttcc ttgcaaatct cgccgatgac catgttgtgg
    19141 acaccgccga tcttgaggat ctcgccagga ccgccaggcg gagtccgggt taccagcacc
    19201 ggatcgtgca gtaccagcag tcgcttgcgg cgctcacacg cgatgtggat acctcgtttg
    19261 cctatcgcta catcaaggac ggcaggacgc tgatcctgcc gctcgctttc ctcggggcca
    19321 tcccctgtgg tttgtccatc ctcctgcttc tcgttgcacc tggcgcggcc tatctcctgg
    19381 tgcccgccgc tgtcctctct ctcatagccg caatccaggt cggtattgcg gccctcttcc
    19441 cctcgacact gtttgggttc tggaagggcg agagttaccg ggaaaaactc gagtgggacg
    19501 cgtttgccag gttcctctcc gatcttgccc tgatcaggga ttactcgccg gaggatctct
    19561 ccatgtgggg ggagtggctt gtctacggga cagcgctcgg tctcggggat aaggtcgaag
    19621 cggcgatgaa gagtctgaat gtgaacctcc cggacgcggg ggtgccgctc tactcgagca
    19681 tgccggtgat cttttcccct atactcctct tctccccgcc atcaggcggt ggcagcggcg
    19741 tggccggtgc tggtggcggg tctttcggcg gtggcggcgg ttttggtggc ggcggtgtcg
    19801 gggggcggtg agacccgccg ggccacaaaa tcctggaacc tcttcatcac gcccctcctg
    19861 gtgaataggt tcgaatttct tcgtagttaa ggcaacctac ttaccgggag cggtagaatg
    19921 gatccctgtt gatgcctatg acgggatcaa gtacaggata cctggttgtt ctggtcaccc
    19981 tgtttggggc catcctggcc tgcgggtgca tggctaccgg gggtgatgag acggtggatg
    20041 gtcgtctctc tggcaatggc acggtcgtct tcatcgacct tgagggcggg ttctacggta
    20101 tagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcac ggttgtcttc
    20221 atcgaccttg agggcgggtt ctacggtata gtcgccgaca agggtggccg ctacctgccg
    20281 ctcaacctga acgagaccta ccgggttgac gggatgcgcc tcaccttcgt cggagaggtg
    20341 aagagggaca ccgcgacgat tcaacagtgg gggacgccgc ttgagataat cgacatcccc
    20401 tgggcgtgcg ccaaatgcgg cgggaacgcc ggtgtcgcaa accctgccgc tgtctggtgt
    20461 gtggagcagg ggcacacata cgagatccgg aagaacccag acggcagcga gtacggtgtc
    20521 tgcatcttcg agaacggcac cgagatcgat gagtgggagt actaccggga gacccactga
    20581 cctttcctgc agccatctct gaaactcccc ggtgggggtt ggggctatcc cgggccagag
    20641 cccccccgga acgttcctga cctctatggt tccagatgct cttccggtag agggaggatc
    20701 tcccgcatca tcttcacggc gcagaggtcg ccgcacatag agcaggtctc aagcccgctg
    20761 tcccgctcgc ggattcggcg ggcctcatcc ggtatgagag ccagctcaaa ctgtttctcc
    20821 cagtcgagca cccgccgcgc ctctgccatc cggatctcgc ggttctttgt gcgcgcggcg
    20881 gcgcgggaga gactccccac gtgtgccgcg atcctcgccg cccgtgtccc ctccacgata
    20941 tcgcggatct ccggcagcgc caggtgctcg cttggcgaga ccatgcagag gaaatcggcg
    21001 ccgttcatgc aggcgatcac gccgccgatc gccccgacca ggtggtcgta gcccggtgct
    21061 acgtcagtca cgagcggccc gagcaggtag agcggggcac tgtcggtcag ttctttgagc
    21121 atcctgacgt tgcagccgat ctgatcggcc gggatatgcc ccggcccctc gatcatccgt
    21181 tgtaccccgg cagcgagcgc ccgttttgca aggtgtccaa gcgtcaggta ctcggtcgac
    21241 ttcgccaggc gcccggcatc cacgagcgcg cccggccgca taccgtcccc aaggctcgcg
    21301 accacatcgt gctccgccag gatctcaagg aggtagtcat actcggcgta gagggggttt
    21361 tcctcaccgg tcgctgccat catcgccacg tgaaacgccc cgccgcggga gacgacgccc
    21421 atcacccttg gatcggcctt gagcgatgcc agagcatcca ggttaacacc gcagtgcagc
    21481 gtcaggaaat cgacgccctg tttgcagtgc tcccggatga cgttaaagag gaggtcggca
    21541 tcgacgtccg cagcgctccc cgcccgtcgg acagcctcgt agaccgggac ggttccaacc
    21601 ggcgcatcaa gctcaagaat ccggcggcgg atgcgggcga ggtctccggc ggtcgagagg
    21661 tccatcaggg tgtctgcgcc ctcgcgtagg gcggtctttg ccttctcaac ctcgagatcc
    21721 tcgtcgcacc gaacgcccga cgttccgacg ttcacgttga cccgcaccct gcacccctct
    21781 ccgatggcgc agaggcggtg cggccgaacg gggtttgccg ggatgacgat ccggccgcgg
    21841 gcgacagtcc gcgccgctgc gtgaggagcg agatcctcat cgcgggcgat cgtctctacc
    21901 tcggaaggaa ccccgcgcag gcacgcttga acaagggtgt gcataagaac catttgtccg
    21961 gaggccatat tagtctgcat gcatgtccgg tggatcacag gtggcatgac ctatgcaaac
    22021 tcgtttgttt acggaaacat actgatcgat gccggcgtcc tcccgatggc ggtggagccg
    22081 tatgccacag atatcgatac gatcgtcatc acccacgctc attacgacca tatcgcccat
    22141 cttcctgaga tcgcggccat gtgcggtgat gccaccgtct gcatccacca tgcggacgca
    22201 ccgggccttg tcgacgacgc caggagtctt gcgatacatt tcggtgcccg gtctccgggg
    22261 atggtcccgg acgtcacgct cgctgacggc gaccgcatag ggacgctcct cgtgattcac
    22321 accccgggcc acaccccggg cgggatctct ctctatgacc gggaggcgag gttgctcttc
    22381 tcgggcgaca ccgtatttac gggcgggtcg tttggccgct acgactttcc gggaggcgac
    22441 cgcagggcac tcgcggcatc catcgagcgc ctcagcggcc tggaggttga ctctctctac
    22501 ccggggcacg gcgagcccgc ccagcgtgac ggttggaggc atatagcagc cgcacgggag
    22561 gcgctccggt tgtatggata agggggtcta cgccctcctc attgagaact cctgctgcag
    22621 ggttcgcatc ggtgccctgg gggtgcggga gttcgctccg ggctatcata tctacatagg
    22681 atccgcgcag gggagcgggg gtcttggccg cgcagaccgg catgtcaacc ttgcgttcag
    22741 ccgtgaccgc cctccccgct ggcatatcga ccacctgctc ctcgacccgc actttagacc
    22801 ggtcgccgtt ataaccgcag tcaccgggcg tgactgcgag tgcgaccttg caaaggccat
    22861 cggtgaccca tccgtcccgg gattcgggtg cagcgactgc aactgcccct cccacctatt
    22921 tcatcgccct ggtgaccccg tccccgagat cctggcggct tttcggcgtc tcgatctgga
    22981 tgcccggatc acaagaatca agaacgcggg agataagcat agggtatgac tgttcttgga
    23041 atctccggaa gcctgcgcaa gaacggcaac accgcgacgc tcgtcaacac aatcctcgag
    23101 cgtgtgcggg aggcgggtgt cgagacggag tacatatcgc ttgccggtat ggagatccgg
    23161 ccatgcaccg ggtgcgagac ctgccgggag acgaagtggt gtgtgacgga ggacgatgac
    23221 tgggggtttg ttgcgcggag gatggtcgag tgtgaggttc tcgttttagg cgctcctacc
    23281 tattactacg acatcaatgg ccagacgaag aacctgatcg accggacata ctcactcttc
    23341 cacgagagga aactctcggg gaggagagcg gttgcagtgg cggtctgtgg cgatcgggga
    23401 tgcgagcgca ccctggagac gatagaaggt ttcctgaacg ctcacgagtt ctcgtatcta
    23461 ggctacgtct gcggcagggg gtatgctgcg ggagatgtcc gtaacgacga gcgggcaatg
    23521 gcgcgggcaa gggaggttgc ggatacgata gtccgctaca tccgcccgga ggattgaggt
    23581 cccgccacca ccgcctctgc ggggtaccac tttctgcgga gaaagccctt caaactcccg
    23641 ggaggggtgc gcaacccgtc ttccccccat ccatatcgcc ggcatcctga gggttaaccc
    23701 gaccctccgg gtgcgattca gccctcaaga agggtgtatg tcgtcgtccg tctccgcaga
    23761 gtcctgccaa tatcctctgc gatcctgcgc atctccgccg ggtccaggta gtcggtgccc
    23821 ctcgcgcctg cctcccggga gatgctctcc tcaaagagcg ttccacccag atcgtcggcg
    23881 cctgcaagaa gcgccatccc cgccatcttt gtccctatct tcacccatga tgcctggatg
    23941 tggtcaaagt tgtccaggaa tagcctggat accgcaaaca acagcagatc ctcccgcccc
    24001 gttgcaccgg gccgggcaag ccccgcacgg tagagcggcg tattcgggtg gacgaacgag
    24061 agggggacga actccgtgaa cccgcctgtc tcgtcctgga tctcgcggag gatctcgagg
    24121 tgtcttgcgc ggtcggcctc gctctcgcag gagccataca tgatcgtcgc tgtcgaccgg
    24181 atgcccatct catgcgcctc ctttatgatc cggatccatg tcgcagtatc tatcttgtcc
    24241 gggcagatca cacgccgtac gtcatcgaca aggatctcgg ccgccgttcc gcagagggtc
    24301 gatagccccc cctccctcat ccgttccagc acctcacggc tggagatgcc gctcttcctc
    24361 gcggcatagg ccacctccat cgggttgctt gcgtggatat ggacacccgg cgcctcctcc
    24421 ctcatccagg agtagatgtt gacgtaggag tctgcgttaa aggccgggtg aagaccgcta
    24481 accgtgcaga tctctgtgac gccgcgttcg cgtgcagccc tggctttctc ccgaaccacg
    24541 gcctcgtcgt gacagaaaga gtcggggtct cccggtttcc gtgaaaaccc acagaacccg
    24601 caggcgttca cgcagaggtt ggtgacgttg atgttctggt tcctgacgta ggtgacaatg
    24661 tctccgactt tccgctcccg gagttcgtct gctgcggcgg cgatcctcca gacgtcccga
    24721 ccgcgcgccg atagcaggct gacagcctgc tcttcggtta gccggtggcc ggcaagcgcg
    24781 tcgtcgagca cctgttgcag tggtggtgtc atgctttcat ctcgccttcc tgggtgcgat
    24841 ggattgatct ggcggggtca ggagaagaac tttcgggtct cccggccggt ctcgatgggt
    24901 ataacccgaa agagcgttcc aggagcattg tgaagagcat ggtgccacac acggcatcat
    24961 gcccctgaat tcagtcgggg gtagtcgacg caacaatctc gtatgtcgtc gtccgctgcc
    25021 ggagcgtcct gccgagatcc tctgcgatcc tgcgcatctc cgccgggtca aggtaatcag
    25081 caccctcacc cccggcctcg accgagacgt cgtcgcagaa catcgtcccg ccaagatcgt
    25141 cgccgcccgc cagcagcccg agttctgcca tcttcgttcc gaccttgccc caggagatct
    25201 ggatatggtc aaagttgtcc aggaagaggc gcgcgaccgc aaagagcagc aggtcttcgc
    25261 gcccggtggc gcctgcgggc gcaagcccct gccggtagag ggcggtgttc tcatagaggt
    25321 aggagagcgg cacaagttcc gtgaacccac ctgtctcgtc ctggatctcg cggaggatcc
    25381 cgaggtgtct tgcgcggtcg gcctcgctct cgcaggagcc atacatgatc gtcgctgtcg
    25441 accggatacc catctcgtgc gcctccttta tgacaccgac ccaggttgcg gtgtcgcact
    25501 tgttcgggca gatcacccgc cggacgtcgt cgacaaggat ctcggccgct gtcccctgca
    25561 gcgtgccaag accagcatcg cgcaggtgtt cgagcgcctc cctggttgag actccgcttc
    25621 gttccgccgc ccaggcgatc tcgtcagggc ttgcggtgtg gatatccacg cccggcgcct
    25681 cctcgcgcac acacctgata aggtcaatgt atgagtccag ggtgtagtcc gggtggactc
    25741 cggagaggat acagatctcg gtgacgttac gctcgcgtgc gatcctggcc ttctcccgca
    25801 ccgtatccag gtcgtcgcgg aacgcgccag ggtcatccgc nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn gccggcaagc acgtcgtcga gcaggtcatt cagcgaatat
    25981 gccatcttaa gcacttcttt tgtgctttca ggacttatac ctctttctct tctcggcgcg
    26041 cgaggatgag atcgtggata attatgatca ggatgacgac agcggcaaat tcaggcaggt
    26101 ggagcgggag gtaacctatg agcggcaccg ctgccagggc tttcccaacg acccattcct
    26161 ccttcaccgg ttcgatcaca ccgatgccgg agatcatgag gtttggctgg tcggtgtagg
    26221 ggttgttatc acctttggtg atgtagccac catgcggttc atcgtagtag gccccgagtc
    26281 ccgactctat caccccctct gtgtcgaggt agatcatcgc ccgatggatg atcgggtgga
    26341 cggagtcggc gccgtttggc cggtagacga tcacgtcgcc gtagtccccg aaagacgagt
    26401 agccaatcat ctggccttcg gcccaggtga cgagaccccc gaaccggttc tcgtcgacga
    26461 caaacaccag gtcgcccacg ttcatgtgcg gaaccatgct ctccgactct atcgtgacca
    26521 ccgccggcca ggttccggca aagagataga ggagaagggc gataccccca acgaccgcga
    26581 cgacccagag gatgtcccgc gcaagcgaga taccggggtg atcgctatcg cggaacgccc
    26641 tcacggcatt tgctgcacgc tgcagccagt ttgtagccgc catctgctct aaagatgtta
    26701 cccatccatc atatacatag attcccaggc tggtcttctc atcggctctg cagggtgaaa
    26761 tgtaaagagg gtgtgacggg atctgcccgc atatcgctgg aattttgtgg atctgatgtc
    26821 aaatccctgt ccctgtcttc attacacggg ggtgtgcgga agggtaaagc caatattctc
    26881 aggggagaat aacatgaagt ccatgcttgc caacgccgag atcgtacgcc ggttccttga
    26941 gacgaagcac caggtccacc ccgacgtggt gaactacatc cgcgagcaga acgatccttc
    27001 gctcatcgac cggattgcgg cggcagccgg tgacgccctg gtggtctcca ccgagcacat
    27061 accgggtctc aagaaggtcc gggatgggac gaggttcctg gtcgaccctg atgttgaggt
    27121 gatcgtgggg agcgccggaa gcagcgggag cgtcagcggc cacgaggacg ccgttcacta
    27181 cttccggaac cgctacaaca ccctatcctc gatgatccgg acccggatga ctgctatgcc
    27241 ggtcgaggcg ctgctgaagt ctcccggccg ttaccgtgac gaggagtgca gcatcatcgg
    27301 gatggttgca gaggtgcgga cgaccccgaa aggccacagg atggtcgagg tggaggaccc
    27361 aaccggggtg atccgggtgc ttttcaacaa ggggcgcgac gagtcgttcg aggaggccga
    27421 acgtatactc cctgacgatg tgatcggggt caagggaaaa ctctcaggtg atggggggct
    27481 cctctttgcc gagcaactcg tccgtccgga cgtccccatc aacaacgccc ctttcaagag
    27541 cgatcgaccc ggaaaggctg tcctgatctc cgacgtccac gttgggagcg acaccttcct
    27601 ccaggatgcc tggaaccggt tcgctgactg gctccaggac tcagatgcct cctacctcct
    27661 gatcgccggc gacctcgtcg acggcatcgg catctacccc ggacaggaga acgaactgac
    27721 gataaagaac atctacgagc agtacgacgt ctttgcggag atgctctgcg acctcccgtc
    27781 ccggatccgg atcgttgccg cccccgggaa tcacgatgtt gtgaggatgg ccgaacctca
    27841 gcctgcgatc ccgcgggagt ttgccgcgaa ttttccgcca aattgcaccc tcgtcgagaa
    27901 tccctgtctg ctgaacctcc agggcgtccg cgtcttgatg tatcacggcc ggtcgataga
    27961 cgacatgatc gggctcatcc ccggcgcgag ttacgagcgt cccggcgaga tcatggttga
    28021 gatgctcaaa cgcaggcttc tcgccgcccc ttacgggatg cggacaccga tcgcagcgat
    28081 gaagaccgat cggcttgtca tcgacccgct cccggagatc ctcctcacag ggcacatcca
    28141 tatctgcggt gtcacacgtt accggggcgt tctcggggtt aacgccggca cctggcagtc
    28201 gcagacgtcg tttcagaaac agatgaacgt ccaccccaca ccggggcggg ctgttgtcgt
    28261 cgacctccag acgctccagc caggggtgat cgattttagt taaacgtttc atccgatctt
    28321 ccgggagttt ttccacatct ttaaaaacgt tgaatccctc tctatatggt actcaatcgg
    28381 aggaatgctg agagatggat ctggtgtatc tggcgcccct gggtgctatc ctggctctcc
    28441 tgtttgcagg atactcgtat ttgagtatca gacgggaggg caccggtacc gaattgatgc
    28501 agaagattgc tgccgccatc cacacgggag caatggtcta cctgaacaga cagtaccgcg
    28561 caatagcggt atttgttgta gtgcttgccg ttgtcctggc actcgtgatc aatcctctca
    28621 cggcggcgtg tttcgtcctc ggtgcggctc tctcggcggc cgcaggctac ataggtatgt
    28681 tcaccgcaac cgccgccaac gtccggacga ccaacgccgc gcggcgcggg gttgccgatg
    28741 ccttccgcgt ctcttttgcg agtggttcgg tcatgggtat ggttgtggtc gggctcggtc
    28801 ttcttggtct ctcgatcgcc tatgtggtca tcagcaccgt aaccgggctt ccccaggctg
    28861 aagtccttac catcgtgacc ggttttggtc ttggtgcaag ttcgattgcc ctcttcgccc
    28921 gtgtcggcgg cggtatcttc accaaagccg cggacgtcgg tgctgacctg gttggaaagg
    28981 ttgaggctgg catcccggag gacgaccccc ggaaccccgc ggtcatcgcc gacaatgtcg
    29041 gcgacaacgt cggcgacgtc gccggcatgg gtgctgacct ctacgagtcc tatgtcggct
    29101 cgatcatcgc tgctatggtc cttggcgtct ctgtcgcggc cacaatgttc ccgaacgtcg
    29161 atatgatgaa cgtgatcctc ctgccgctgc tgatagcaac ggtcgggatt gttgcgtcca
    29221 tcatcggctc gttcttcgtt aggaccaaca agaccgagag cagcgccatc cacatggcct
    29281 tcaacaaggg gctgcttgct gcaatcattg taaccgtcgt tgctacctac ttcctcgtga
    29341 caacgcttct cggtgcagag tacatcggag tcttctacgc cactgtcgcc gggctcatag
    29401 cgggtttcgc catcggcctg atcacggagt actacacctc ctttgagcgt aaacccaccc
    29461 aggccattgc aaagtcgacc gagacggggg ctgcgaccac catcatcacc gggtttgcaa
    29521 aggggatgga gagcaccatc tggccggttc tgatcatctc tgtcgcgatc ttcgtctcat
    29581 accaggttgc cgggctctac ggcatcgcga tcgccgcggt cggtatgctc gcgacgctcg
    29641 ggatctccct ctcggtcgat gcttacggcc cggttgccga caacgccggc ggtatcgccg
    29701 agatgtccca ccagaagccg gaggtccgcg agatcaccga cacccttgac gccgtcggca
    29761 acaccaccgc cgccatcggc aaggggtttg ccatcggttc agcagcgctg acggcgctcg
    29821 cgctcttctc cgcgtacagt caggctgtgg gcctctcggt aatcgacgtc tccgtcccga
    29881 acgtcttcat cggcgtcctt ataggtgcga tgctgccgtt cctcttctgc tccatcacga
    29941 tgatggcggt cggtaaggcg gcatacaaca tcgtccatga ggtacgccgc cagttcaggg
    30001 agatcaccgg gcttatggag gggaaagccg atcctgatta cgccacctgc atcgccatct
    30061 ccacgcgctc ggcgctgcga gagatgatcg cgccaggcgt ccttgccgtt gccgccccgc
    30121 tcgtcgtcgg ctttgtcctc gggaaagagg cgctcggcgg gctgcttgta ggctcacttg
    30181 catccggttt cctccttgcc gtcacgatgg caaacgccgg gggagcctgg gacaacgcaa
    30241 agaagtacat cgagcagggt cacctcggcg ggaagggatc ggacgcccat aaggcggccg
    30301 tcgtcggcga caccgtgggc gatcccttca aggacacctc ggggccgtcg ctcaacattc
    30361 tcataaaatt gatgtccatg gtcgcgctgg tctttgcacc gctgatcctg gtcatctgat
    30421 catcacctct tttctttttg cacggcgcta tcaacgtcac cctcccatac tcttcagggc
    30481 ttgcccttct gcaggtgtaa aataagaggg gttttgctcg tcttctggca acttgcccat
    30541 gcgcggtttc gctcttctgg agaccaaaac ctttatccca aaatatccat tattaatcat
    30601 gtaaaatctg tagcgatcgc gcctggccgg ggttgttaac ccgatgggaa ccggcaccac
    30661 gtgccggcga catgtgcatg gtgagggcga ccacgggccc cgggaggggc ccgggagatt
    30721 gataagaatg gactgaggag atatggcaga ttcgttcgcc gtgaaactgg aagagaagaa
    30781 gcactacccg gacccgcgtc tcaaggccag cgcctgggta accgactacg cccggacata
    30841 tcaggagttc ctgcgcgacc cggcggggtt ctgggagggg atcgcccgcg aactctcctg
    30901 gtttgagccg tgggacaggg ttctggagtg gaactacccc tacgccaggt ggttcgtcaa
    30961 tgggaaactc aacataacct gtaactgcct ggacaggcac gtttccagca gcaggaagga
    31021 caaaccggcg atcatctggc atggggagag tggagaaaag cggatcctca catacgaaga
    31081 cctgcatcgt gaggtgatgc ggtttgcaaa cgggcttttg agcctgggga tcagaaaggg
    31141 cgatcgggtc tgcatctaca tgccgctcgt cccggagcag atagtcgcga tgcttgcctg
    31201 cgccaggatc ggggccgttc actcggtggt cttcgggggg tttggggcgg gtgcgctttc
    31261 gatgcggatc aacgacgccg aggcgaaggt tctgatcacc gctgacgtcg gttaccgccg
    31321 gggaaagacc gtccctctcc gggagatcgc gcttgaggcg ctcgctcagg cgccatcgat
    31381 cgagcgcgtc gtcgtcctca ggcgggcgag gccggccgtc gagctcgacg gtgtgcgcga
    31441 ggtggactac gccgaactta tggcccgttc cgcgccggaa tgcccgccgg aggtgatgga
    31501 cgccgaggat ccgctcttca tcctctacac aagcggttcg accggcgcac cgaaagggat
    31561 cgtgcacacc tgcggcgggt atgcggtcgg gacgtactac acgacgaaat gcgtcttcga
    31621 catcaaggag aatgatatct actggtgcac cgccgacacc ggctggatca ccggccacag
    31681 ttacatcgtc tacggcccgc ttgcggtcgg cacgacagtt gtcatcgccg aaggtactcc
    31741 cgactacccg gacccgggcg cctactggcg gatcgtccag gatctcgggg tgacgatctt
    31801 ctacaccgcg cccacagcca tccggatgtt catgcgctac ggcgatgagt ggccgaagaa
    31861 ctacgatctc tcatcgctcc gcgttctggg ctcggtcggg gagccgctca accctgaggc
    31921 gtttgaatgg ttctaccacg tcatcggcgg cggcaggtgc ccgatcgtcg atacctggtg
    31981 gcagaccgag accgggatgc acatggttac cacgatgatc ggcgagccga tgaaacccgg
    32041 gtttgccggg aaacccatcc ccggcgccgt tgtcgacgtt gtcaaccgcc acggcgagcc
    32101 ggtcgctccc ggcaccggcg ggttcctggt cataaaggaa ccgtggcccg caatgctccg
    32161 gacggtccac gggaacgatg agcgttaccg cacctactgg aacaccatcc cagggtgcta
    32221 caccgcaggc gacctggcgg tgaaggatga ggagggctac atcatggttc tcggccgtgc
    32281 agacgacctt atagtcgttg ccgggcacaa catcgggacg gccgaggtcg agagtgcgct
    32341 cgtctctcac agggcggtgg ccgaggctgc agtcatcgga aaacccgatc ccctgaaggg
    32401 gaatgtcatc aaggccttcg tcaccctctg tgtgggggtg gctcccggag acggtctcac
    32461 ggctgagctc gcacgccatg tcaggaagaa cctgggcccg atcgcggtgc ctgccgagat
    32521 cgagtatgtg gatggactcc ccaagacccg gagcggcaag atcatgcgcc gtgtcctgaa
    32581 ggcccgcgaa ctcggcatgg acccgggcga catctccacc ctggaggagt aggtctccat
    32641 gcccgataca acaccagcga tctttggtat gcccgcagag aagggacggt gggggctcgt
    32701 cgccctgggg ctcataatca acctctgtct tgggagcata tacgcctgga gcgtcttcac
    32761 cgcgccgctt gccgcgcact tcacggaggt gctcggccgg catgtgacgg ccggggaggt
    32821 cctcctcccg ttctcggtct tcctggcgtt cttcgcgata gcgatgccgc ttgccgggaa
    32881 gtacatcgaa cgctacggcc caaggaatgt gacgatagtc ggcgggatcc tcaccggctt
    32941 cggctggctc ctggcatcga cggtatcttc ggtcgaggcc ctatacattg tctacggggt
    33001 gatcggaggc ctcggggtcg gcatcaccta tggggtgccg gtcgccgtgg ccacccagtg
    33061 gttccctgac cgcagggggt ttgcggttgg gctcacactg ctcgggttcg ggttctcggc
    33121 ctttatcacc gcaaacattg ctggagccct gatcgctgcc tacggtgtta tgacgacatt
    33181 ccggatcttc ggcatcgcct tcatcgtcct gctggttctc tcggccctgc cgctacggtt
    33241 cccgccggcc ggctggcgac ccgccggctg gacgcctccg ccgcccggca gtggcagcgc
    33301 accggcctgt gagtgcgacc ggcgcacgat gctccggacg gggacgttct acgggctctt
    33361 tgcctgttat ttcatcggct gcctggccgg gttgacagcc atctctatcg cgcagccggt
    33421 tggaacggag gttgctgggg tcagcgccgc gctcgcgaca gctctcgtcg gcttctttgc
    33481 catattcaac ggccttggcc gcccggtctt cggaagcctc accgaccgta taaccccgca
    33541 gaagactgcg atgctgactt ttgcgcttat agcggcggca tctctcctga tctggcttgt
    33601 gccgggggtg ccgtcctaca tcgttgcgtt cgccatactc tggggatgcc tgggtggctg
    33661 gctcgccatc gcgccgacgg cgacggcctc gtacttcggg acgtgcgact acccgcgctg
    33721 ctacggggtt gtcttcctgg cctacggtgc gggggccatc gccgggccgc aggttgccgg
    33781 gttcatccgg acggcgagcg ggacatacct tggagtcttc ccgctggttg cggcccttgc
    33841 cgtcgtcggt tttgtcatcg cctggctcct gatgcgcccg ccggccgggg ttccggcgcc
    33901 ggagaaggaa aaagaggagg gtctttaagc cctcttgata agcccgagct gccggagcac
    33961 ctccgcggcc ggcaccatac cgccgtccag ataaacagcg cctatgagcg cttccaggca
    34021 ttcggcgaga acccggccgg atgtccagac cctctgggcg gcctcaccct ttccccaccg
    34081 gacatgctcg gcaaggccga tctcttctgc aagggcgcgg agcctggaca tgttcaccag
    34141 gttcatcttc cggttggtga tcgcgccctt gtcgtgcacc ccttccgcca ccacagcctc
    34201 cacgaccgcg acgttgatca cggcgtcgcc gagggttgcc agggcgtcca tgtgcatctc
    34261 cgggggaaga ccggtctcca gggtgcaggc caggcgggtt agcgcccgct caagcaggga
    34321 ggggttgcgg aatgtgtagc caatccgctc ctctaccgcc cgcaggttgc ggttgctgtt
    34381 ctcccactga ttcatcgctc cacgacttca gccttcgtta tccggacggc gacctttggt
    34441 ctgtcagcgg agtccgtctt caccttcccg atcctgtcaa cgacgtccat ccccccgacc
    34501 acctcaccga agaccgggtg ggcgctcggc gtgcgtgggt cgtcccagtc gaggtaggtg
    34561 ttatctacca ggttgataaa gaactggctg ccgcctgtgt tcgggcggcc ggtgtttgcc
    34621 attgcaaccg tcccccggac gtttgagtgg cctttcacga actcgtcggg gatggtatag
    34681 cccggcccgc cgcttccggt cccggtggga tcaccaccct ggatcatgaa cccgtcgatc
    34741 acacggtgaa acacagtgcc gtcgtagaaa cccgaacgga ccagtttttc gaagtttccg
    34801 gcggtcaccg gcatatcatc gtagagccgg atcgttatat cacccattgt cgtgtggagc
    34861 acgactctct tctctgccat ggccattagc tccgtatgat attgtccgct gccgcattta
    34921 tactgcggcg ggggtgtcgg cgcggggccg gccgcgcgtg ttaactatat gtgcccaaca
    34981 atgcctgcaa gtaaaactga tttatacagg cagcccggaa gtatatgtat gtactctgga
    35041 gcgaacgtct actcccggtg ccggcggagg aggcgtctcg tatgaacggc agggcgccgg
    35101 tcttcatcat caccggcggg cagggtcagt gcaagacgac gttcctgcac caggttctgg
    35161 ggctcaccgt cggggcaaac gttcgggtga gggggatcat cgccccaggc catgtgcggg
    35221 acggccggag gtcgggtttc acgctggtcg acctggccac cggtgaacac gaggaactct
    35281 gctctatcga tcccgatcct cggtgcgagg ttcacggccg tttctacttc cggccggaag
    35341 cgctcgcgtt cgggcggaga gcgctcgcgc cgcccgatcc ccggtctacc gatctgctgg
    35401 tggttgacga ggttgggagg tttgaactgc agggtgcggt ctgggcggac cgcctcgatc
    35461 gactgatcgg tcatcctcat ccaccgatga tctggacggt caggcgcaga ctgctccaca
    35521 cggttgtcga gcgctggaac atcaagaacg ccgtcgtggt ggacgtgagg acagcgagcg
    35581 tcatggcgac ggccgatgcc gtgatggacg cgatatggga gtggcgggag gcgcaggcgt
    35641 tctccagggc cacaccagca gcaccacagg cggattgcat ccccggtctc tctttgctgg
    35701 ttgcggatgc ggccgctgcc ggagcctcac aaaacagccc gataatcctc cggagatgct
    35761 atagcccata ggggtggtgt gggtcttcct cctggcccgc acgtcacgtt cgaaagttca
    35821 ggcaacaaca acgcgcgctg tcaagaacat actgcccgct ctttttcata ccctggatcg
    35881 cgcataagac ggagcgaaga tgctgcacgc atgaatgcag cctgttttga tcgcaggtgg
    35941 ttcgcaggag agatccgact tgtcttcccc gccgggctcg ccgccgatgt attgatgggc
    36001 cgtggggact ggtcgcgtcc ggccatccgg ccggctcacc ccccttttga tccggcaccc
    36061 catgcaccag tggttggagg ttatgactcc ggttgtgcgt ggttcagcac cacgtccgga
    36121 atgggtttca accaaacact tttaccgttg aagtaaatgt tatatcattt acattaacca
    36181 atgattaaat aaacttcata tacaaacgtg gtatgagagc ggtgcgcgag atgacctgcg
    36241 ggagagaaac aatcacagaa gcggggcagg agccaaaaag gaacatcacg accaatcctc
    36301 cgcgtttggt ctcccgcagc gcgacatcga tctctgagac cgtcctccgt ctcatcccgg
    36361 tagagagggc gatctatatc ctcccggttg tcctcttatt cgtctccctg ttcgtcggga
    36421 ggtacatgat ggaccctgtg gagaccttcc ggatcctcgt catagggtcg gcgaacgcgc
    36481 tcctcgatgg ggcatcaacc ctgctctcca cactctcagg tcatcaggtc gtcctcccct
    36541 ccttcgagca gacctggggg gtatcagagc agacggttgt cttccggatc aggcttccgc
    36601 gggtgatagc ggcgatgctc gtcgggggag gactctccct cgccggcgca tcgttccagg
    36661 ggctcttccg gaaccccctt gtctcacttc ctcccgcccc ggagggccgc gatcacggcc
    36721 gccgccagct gttcgatctc gtcgaacctc ggctcctcca tcccgttttt cacgatcccg
    36781 cactcggtcg cgacgatgtg gaggtcaggc accaccccgg tctcccggag ttttttgagt
    36841 gcacaggcat cttcgcagcc gtccagggcg agaattctgc cgcatggcgg ttcctggagc
    36901 ctggctgccg ggatacactc ctcaacgctg cccggaaacc gctgcaggac caccattccg
    36961 gccctggcgg tcaccttccc tgtgctggag agcccggagc aggtgaccag ggttatgggc
    37021 tccatcgcag catcactcca tctcgagggc tcttgcgacc gcccgcccga tcagttccgg
    37081 cgggatggcc tcgtagtcct cgccgctgta gcggagcgcc ctgcactccg tatcctcctc
    37141 gcaggttatg caggcgcagg aggcgcaggg tgtcgagatc acctccacgc cttccagcag
    37201 ttcctcgatc ggtacaccgt tgaagaggag ggaatttgac tcttcgacgg ccgttgccgg
    37261 gagcacggtc tcgatcagcc tgacgctgac accttccatc tcaaggattg tagtgatctc
    37321 cgagatgacc gctttgagag ccgttccagt atcgtggcac cgttcgcagg tggtatcgag
    37381 atcctctcca acgtgtttcc attcgatgat aagttctttc ttcatgctgt atctcctcca
    37441 ttggcatcga cgggacatct tcctgtcgcc tcctcaccat accaccgatc gcggagtcgg
    37501 agcgccgcgt tcaccaggga catgagcacc ggcacctcga tcagcggacc tatgacggtg
    37561 gcaaacgcca cccccgaccc gatcccgaag atccctatgg caacagcgat ggcgagttcg
    37621 aagttgttgc tcgccgccgt aaacgagagc gctgttgccc tgccgtaatc caccctgagt
    37681 ctccaggaga gccagaacga gacgaaaaac atcaacagga agtagatcgc aagcgggatg
    37741 gcgatccgca ggacatccag gggaagtgcg acgatgtttc cgccctgggt tgagaagatc
    37801 acgacgatcg taaacaggag ggcaaagagc gtgatcggcg atatacgagg gataaaccgc
    37861 gtatcatacc agatatgccc tttcagttgc aggagcgtaa cccgtgtcat aaacccggcg
    37921 atgaaaggga tgccgaggaa gacaaagact gttgccgcga cgctcgcgat cgttatcggc
    37981 accgctgtac ctgccgctat gccgagaagc gggggcagga ctgtgataaa gacgtaagcg
    38041 tagagggcgt agaagaatac ctggaatacg gagtttaaac ccacgatcgc ggcacagtag
    38101 tcgcggtcgc cgcatgcaag gtcgttccat acgatcacca tcgcgatgca ccgggcaagt
    38161 cccaccagga tcagaccgta catgaactcc ggtcggcctg caaggaagac cgctgccagg
    38221 aagaacatca gaaccgggcc gacgacccag ttcagaacga tggagagacc gagcaccttt
    38281 cggtcgcgaa agacccgtcc cagatcctcg taacggactt ttgcaagtga cgggtagatc
    38341 atcaggatca ggccgatggc gatagggatc gatgttgtcc cgaccgagaa cccggtgatt
    38401 gctgcaggca ccccggggaa gaagtagctg attgcgattc cggcgcccat cgcaaggacg
    38461 atccagaggc tcaggtagcg gttgaggagg gagagccgcg aaccggcagc gttctccgtc
    38521 tgtatcttgt ttcggccgct catcatcggt cacccccggt cggcgtgaaa cttctgccag
    38581 ggtgcggcgg catacagcct cgcggcaccg gttgggattt agtcatgctc gcgcaccatc
    38641 gaaaccagtt ctgcgccgag agcggtgtca tctctgatct ccaggcggcg acctctgcaa
    38701 agaccgcatc aagcatcttt agccccacaa ttgaccccct gactctggcc ctttccggtc
    38761 acatgccgca gggcgtctgt ggcagcaggg tatacagtct catctccaga ttcatcctgt
    38821 ctgctccatg gtctgttcag atcacgctga gcacctggaa gaggacgccg gcaaggatgg
    38881 cgaccgtgag gatcgtcgcc acaaaggcgg cgaccagtct ccgctcaaag attgccgcaa
    38941 gaagcgtcac ctccggaatg ctcgcccccg cgccgccgat gatcagcgcc atcacggcgc
    39001 cgatccccat acccttctcg agaaggacgg cgctgatcgg gatgagcgtc tccgcccgga
    39061 tgtacatcgg tatccctatg accgccgcca gtggtatggc gagggggttc tccggtcctg
    39121 cgaccgcgat gatcaggtcg ccggggacga acccgtagat gaacgctcca atcccggcgc
    39181 ccaggatgag gtaggggaag acctgccgaa agaggttgac cgcgaatgag aacgctctct
    39241 ggatgcgggc gccgtggctg ctcgcgcagt cgcagggttt cgggcgtcct tcgaccacca
    39301 cgtccctgac gtatcttccg tagccgaacc gctcgaggat caggcctata acgactgcgg
    39361 atgtgaatgt gatggcggcg tagatcacgg tcggcaccac cccgacgagc gcgagcagca
    39421 gcgccaggat caccgggttt aagagcggag aggcaaagag gaacgacatg caggccccga
    39481 acgggatccc aacttcaagg agccccagca ggatggggat ggtcgagcag gagcagaatg
    39541 gggtgagtgc ccctaacccg gcagcgacga tatttcccac accctgccgc ctcccgccaa
    39601 ggagacgccg tatccgctct tcagggatgt aggcctgaag caggccgacc aggaaggtga
    39661 tcccgataaa gagcaggatc agttcggcgg ctatctcaac gaagaactgc ccggcgacgg
    39721 caagtggggt caggatatcc atccaggttg ctccggtgcc gggcgtttac tcctctttcg
    39781 ttgctgaccc ttccttcttc tccttcggga caatcctggt gccacagcag ccctcgctgc
    39841 agccgcatcc ggaggaaaag atgctctttt ttagccttga accgattcca tcagcctttt
    39901 tattctcact tttgtctgcc atgatcatca cgtccgtcct ggcgggtttt cccagagttt
    39961 caattttgtt tgaattactt ctgtgccaca tgggtatcaa gttttctaat tcaacaaaaa
    40021 tcgaactttc ctgaagttgc cggagtttac cgaaaggccg gtgcatgacc ctggccctcg
    40081 ccaaaactgc tatctcgatg ccccgaacgt cgtgtcgatc caggaggtga tctcgtcccg
    40141 gatacgccgg attccggcca ttatctcctc gtcggtgccg gtgagtcccg atgggtccgg
    40201 gaacccccga tgtatctcct ccaccgccca ggggaagata gggcagactc ccccttcgca
    40261 gacggcgacg aggacgtcca tttcccgccc gtcgaactca ccgagatcct ttgcgcgatg
    40321 gccggagata tcgatcccga tctcggccat ggctctgatc gcgtaaggat tcagtctgcc
    40381 gggtctggtg ccggcggaga agccctggta acggtcgcca tacctggcgt tcaggcagcc
    40441 ttccgccatc tgcgagcggg cggagttgcg ggtgcagaca aagagcactc tcttcttcat
    40501 cacatatcca cctcatacct gctctcgata agaggcgcct ctgaatatgg ttttcgcctc
    40561 ccccgacgga agcgggtgta gacccgtgaa ccgtccccga gaccgggtat ttacccggca
    40621 aaaccgtcag cagtctcacc cacctccagg gcgacccccc tccccctggt cgtctatctt
    40681 ttgaggccgg ggtgttgaac ggacgggatg cattcaggga ggatagagcg tgggccagcc
    40741 tggaaaaaag gtatatactg tttcttttca taccactgtg aatcggaaag gttacagtga
    40801 cggcaaagaa acgagagtgc gtggaagatg tccacatccc cccggaggtc gaggagtctc
    40861 tatgccggtg cggcggtatc acaggactgg tggagcagtt gccgcgtgat gaggcgctgg
    40921 agcgggagag cagcatccac cgcgtgcttg ccgacccgct ccgtctcaag atcctggcga
    40981 tgctcgccat ccagcctctc tgcgtctgtg taatcaaggc ggtgctcggg atagccgatt
    41041 caaaactctc ctaccacctc tccgtcctga agaagacggg gcttatcacc ggcgaggcgc
    41101 aggggaactg gatcatctac agcctcaccg agatggggcg cagggtctgg acgcggcgga
    41161 ttcgtgcggc cggaaaccag gaaaaagagt gagtttccgg cccttatacc ccgagaccct
    41221 ctaagaacat ccggtgcagg cgcaagtctc cccctagttc ggggtggaag gcgaacgcca
    41281 tatgccggtc gtgccggacg gcaacaatcc cctcggggat gcgggcaagc acctcgacac
    41341 cgcgacctgc ctccgttgcc accggcgccc ggatgaagac cgccctgaaa ggttcttcaa
    41401 gccccttcac ctccagcatc gcctcgcggg agtccgcctg ccgcccgaag gcgttccgct
    41461 cgacacgcat agggatgagg ccgagaggcc gcacacgggg gtcatccacc ctctctgcca
    41521 caagcaccat acccgcgcag gtcgcaaata ccccgccctc aaactccctg agcgcctcat
    41581 acaacccgtt cttctcgatc agcctggaga tcgtggtcga ctcgccgccg ggtatcgcga
    41641 gcgcgtcgag ccctgccagg tctgcagcct ttcggacggg gacgacagag gaacccggcc
    41701 tctcggtgag agcctcccga aatgccgcga tatgctcggt tacatcgccc tgaagtgcga
    41761 gaacgccaac tttaacgtcc acgggtctgc agcacctctt cttccctgag cgtgtggaca
    41821 tcgaggcctt tcatcgcctc acccagaccc ctgctcaccc cggcgattac cttcgggtcg
    41881 tcgtagtggt taaccgcctc cacgatcgcc ctggcggtcg cctccgggtt tgaggacttg
    41941 aagatccccg acccgacgaa gacaccgtcg gcgccgagct gcatcatcag cgccgcgtcg
    42001 gcaggggtcg cgataccgcc ggcggagaag ttcaccaccg gcagccgtcc gagtttcgca
    42061 cactcgatga ccaggtcagc aggcgcctcg atctcgcggg cacgggcgac gagttcctga
    42121 gtgtccatac ctttcaacgc ccggatcccg cccatgatcg cccgcatgtg gcggaccgcc
    42181 tccacaacgt tgccggttcc tgcctcgccc tttgttcgga tcatcgccgc tccctcgttg
    42241 atccggcgca gcgcctcccc gaggtcgcgg gcgccgcaga cgaacggtat cgagaacctg
    42301 gtcttatcga tatggaactg ctcgtccgcc ggggtcagca cctcactctc gtcgatcata
    42361 tccacgccga gggcttccag aacctgtgcc tcgacgaaat ggccgatcct gaccttcccc
    42421 atcaccggga tggagaccgc atccatgatc tcgattatct tctgcggatc ggccatgcgg
    42481 gccacgccac cggttgctct gatatcggcc gggacgcgct caagtgccat gacggcgacg
    42541 gcaccggcat cctcggcgac ctgcgcctgc tctgcgttga ccacgtccat gatgacgccc
    42601 cccttctgca tcgacgcaaa gccgcgcttg atgagctcgg tgccaaatct gagttcctca
    42661 agtttcatta ctcatgtata gctcatgaac cacaataaaa atactgtggc tatctcaagg
    42721 atggcaacca ggatcactgc cgctctgacc gcagagacaa tcccggcacc cgcctcttca
    42781 agcgttctct ccgggttgcc tatggtgtag acaccgggtt tctcgaacct gatcccaacc
    42841 ccgccggcga tgacggccat cgggatcccg ccattgaacc ccggtcgatt ctttgcatcc
    42901 cggcggagca cctccagcgc tggcatgaac cggccctttg ccgcaaagta ggctagaagg
    42961 accagcccgg tgagccttgc cgggatgaag tttgctatat catcggcacg ggccggaaac
    43021 caccctaacc ggagcctttc gtcccggtag ccgagcatcg catccatggt gttgatcgcc
    43081 cggaataccg cggcgcccgt aagcccgaat agaccgaagt agaggagcgg cgagacgatg
    43141 ctgtcgacaa ggttctcggt catggattca tatgcggccg agaggacgtg ctcctgatcg
    43201 aggtgcgccg tatcccggct caccatcatc ccggcggatg cccgaccggc atcgatcccc
    43261 ccctctttga gcgcccgaac cacggccgcc gtgtgctctt caagcgcccg ccaggcaaga
    43321 gtggccttga gcaggaaggg tgcgatgatg aggtataccc acagcggcgc cgcaacctgg
    43381 acgagggcaa acggtgcggc aaagagcgcg gcggtcagaa aagccccggc aacccctgcc
    43441 gcccgctgga ggctccgggg gtaacgcgcc ggaacccccc accagccgat gaaccggccg
    43501 aggagcgcca caggatggta tcgggactgc ggatcaccga ccagacggtc aaccagcagc
    43561 gccgcggcaa ctacgaacgc cgccgcagcc atgcaaggat gaccccgagg agcagcgcca
    43621 cgaacgcagc ggcgttcagg agatcagccc ggtagacaac ccagagcccg tagagtacaa
    43681 cggcggcaat aacgaggatg aggatgctga gggagtaatg caaacccctc gacggggcaa
    43741 gttcggccgc ctgagcgggc gtcactctcg gttctggcgc cggttccggc cgccccctga
    43801 cgacgacccg gaactcctgc cgtcgggtcc cgtagccggt gacgacctgg agggtaaaga
    43861 aacctggagg agcgtcttcc ttgataggta tgtgaaactc ctctgtcccg ttcacgtaga
    43921 ggttctcgtg gaagaatccg gtgaaggccg aggagttcgt tgaggcgagg gtgatgtgca
    43981 gcggcgaacc gtggttgatg agatccagga cgagatcgct ccccaccgtt acctcaacct
    44041 cctttggcac ctcaatggag ttgatccccc ggcggtttag atgaatctca ggcatctgct
    44101 ctcttcagtc ctcacaaccg gtctgcggga ggaggtttgg gataccctcg cttatggggt
    44161 aatcgacgcc gcacgctgga cacctgaggc tgccctcaag cacctcctcc ccgctctctt
    44221 cggtcacgga gagcgtgagt tcactcttgc agacagggca gcagaggatc tccatcaggt
    44281 tttttctcat acttcacccc tcagatcgat gatctgggag agttcgggcc cg
//
LOCUS       DAOSZX010000004        34813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1018_length_34813_cov_20.686720, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000004 DAOSZX010000000
VERSION     DAOSZX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 34813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34813
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_34813_cov_20.686720"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK196_00795"
     CDS             <1..226
                     /locus_tag="PK196_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HON39994.1"
                     /translation="PASGLVDMELLARAAGIRNTCKVQDEEELEEAWESRGRGPNFIH
                     VVLNPGNAPVGNIPLAPAEIRERFVRALGR"
     gene            664..1404
                     /locus_tag="PK196_00800"
     CDS             664..1404
                     /locus_tag="PK196_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HON39995.1"
                     /translation="MNRNIGARVLSVLLVTAFMGMACMAPVSAGTGDDSDAISPEFGV
                     EYKQNYNTGNNLPNSPAIAEGFYEVFDNAGWGCNIYIGDWDSDAAHVDSVDLAFWAGH
                     GDDSCILLDGLPLVLAWVYFHDCNWGDQDLEWILLHSCHTAQVPGEFKAWPHWALNGA
                     HLVCGFDTVGYDYAQDGAAVANKLFNNYKVRHAWYHALDETHQSNVWIGIIGENAACG
                     DDRIWGEGSVITDPPVDSEIYRCLLQCN"
     gene            complement(1456..2244)
                     /gene="minD"
                     /locus_tag="PK196_00805"
     CDS             complement(1456..2244)
                     /gene="minD"
                     /locus_tag="PK196_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HON39996.1"
                     /translation="MVKVYTIASGKGGTGKTTVTANLGAMLARYGKKTCILDADVGMA
                     NLGLILGLDELPVTLHEVLAGKARVRDAIYEGPFGVKVVPSGLSLQGFQHSNPDRLKD
                     VMTDLVSEFDILILDAPAGISRDGVIPLTLADGVILVVNPEISSIVDSLKTKILTETV
                     GGHIEGAIINRVASSGNEFNTAQMEKLLGTRVIGVIPEDPNVRRASAGRTPVVVKYPA
                     SSASRAFRRIAADVAGIEYIEEETAGVKESFVERLARALFRSKT"
     gene            complement(2276..2734)
                     /locus_tag="PK196_00810"
     CDS             complement(2276..2734)
                     /locus_tag="PK196_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39997.1"
                     /translation="MTGFAAITYYLAEISRALRMAGPPQVPLPPAGTASPASKEPSIE
                     PPLKPGVMGSLEEALGLISRKYSLESITLATADGLLIASTKPGSEDEAARYSHLYTQG
                     KLRGENGIELLGMSHRGETVIAITRSSAPISDDQRDALEKDIQNTLQHWV"
     gene            complement(2809..3558)
                     /locus_tag="PK196_00815"
     CDS             complement(2809..3558)
                     /locus_tag="PK196_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON39998.1"
                     /translation="MQLPRGRFHKIIKSTATPALIEEMRAAKFTGICRIVLGSESATL
                     VLNEGSVVLAEYGGLNGCQALDGVLGSEEEAGVELNLLTPEQVRLSQEFNRQCAIEVP
                     ATGKSGKSPAKRGVPEVAKPAAPGKQPAGSGIHREVKPVTDVKPAKTSKAGTVPERSG
                     TAPGVGKSSPAHSTHSPEPAADSHGIPMPGAKPAQEAAETSQPEGDEIDTLLRNMEEI
                     NIEHLVSNFRTNCKEMLKKIHLDHLIKDNET"
     gene            complement(3764..4243)
                     /locus_tag="PK196_00820"
     CDS             complement(3764..4243)
                     /locus_tag="PK196_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HON39999.1"
                     /translation="MHENRVCIIGCGNPIMGNDGAGILAMHLFEGRFPGVDAIDGGSG
                     GFGLIALMEGYDKVVIVDAMVGIGDCAGDVLTFENPPTGGFHARISHDIGIGDAVAIA
                     KELGYTAEIVTIGIEVGEIQAFSREIDPAVRVGIRVAEEKILAILEDWIDCSGTIRQ"
     gene            4342..5136
                     /locus_tag="PK196_00825"
     CDS             4342..5136
                     /locus_tag="PK196_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HON40000.1"
                     /translation="MIHIVPKPTDTPEDNSTGAVIRELERAGARYRLLDLAAIDPLTS
                     GLEHEHIWVCGIRQDLHQFETLNVLSLSNRIINTPAAIATCASKVMTTALLLQRGVRT
                     PATVYVSSEEQARDFLMRHGKVVYKPVYGYDGNGIRLVTEPGDLGPGPWYLQEYVPND
                     RDFRVFVLGGEAVGAITRVSRGLMHNIHQGGIGMPVEIDDDMRAIAEASAAVVGVDYC
                     GVDLLCDREGYTVLEVNGTPNWHCMAAPIPKLLASYLIECERTMRS"
     gene            complement(5185..5469)
                     /locus_tag="PK196_00830"
     CDS             complement(5185..5469)
                     /locus_tag="PK196_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HON40001.1"
                     /translation="MHKEELIALHGILTEIKDFFELQNPDLKFSQYYALKIDPSQVHK
                     SKMEHKYAIFVLGTELANAMKDVEFASSSRISARMKELADKTLKDLEYLQ"
     gene            complement(5535..6746)
                     /gene="dnaG"
                     /locus_tag="PK196_00835"
     CDS             complement(5535..6746)
                     /gene="dnaG"
                     /locus_tag="PK196_00835"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HON40002.1"
                     /translation="MYSPDTTKYLIHLTLQIEGVVDKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQITTKRGETKGEILISSSLDRAETALLASSLETIDRVGPCTAHV
                     MVDRIEDIRVTKRRRIVERAKEILLENFDEGSINSDELLDEVREAIRIEKLEYLGEER
                     VHAGPHVMVSDAIIIVEGRADVINLLRHGIKNAVAVEGTNIPQIIIDLCTQKTATTLL
                     DGDRGGDLILRELLQVAEIDFVAYCPRGKSVEEMSRKEIVKALRNKVPAVGLIEHIPL
                     EEATERLPVHATPAAVRSPGGEDDQPPPGGGGKKSPSTLGEHMADVRNKKIARFLSPD
                     YTVLLESSAADVENALQNLNGDVEGLVVDRVIDQKLLDQLGGKDLEFVAATDFKGIIK
                     RPLSIRLIKIV"
     gene            complement(6733..6915)
                     /locus_tag="PK196_00840"
     CDS             complement(6733..6915)
                     /locus_tag="PK196_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40003.1"
                     /translation="MKPFPGSDYVLKKVAVRLPSDLSNHSLNIYLSVCPRIQKPVENT
                     EIWITFTEEAGLHVFT"
     gene            complement(7014..7331)
                     /locus_tag="PK196_00845"
     CDS             complement(7014..7331)
                     /locus_tag="PK196_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HON40004.1"
                     /translation="MDTYANAVSETPDGVTIALDVTAGAKRSLFPAGYNEWRRSIRCQ
                     VAAPAVGGRANRAVIDLLAKTFEVSRGNVTIVAGHASSSKVVAVSGLSRSRALECLNR
                     SDA"
     gene            complement(7536..8798)
                     /gene="pyrC"
                     /locus_tag="PK196_00850"
     CDS             complement(7536..8798)
                     /gene="pyrC"
                     /locus_tag="PK196_00850"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HON40005.1"
                     /translation="MLPVTADLLLRNVALPTGRRADIAVTGGVVRHVGAAVRADETID
                     CSRFTCLPGAVDMHVHMRGGVQADKEDWRSGTVSAVAGGVTLVVDQPNTVPPITTPDR
                     LRARIREAGEQAVCGFAINAGVLPDADLTGMWKAGAMAFGETFAAPSSYGEGLDLESL
                     RSLFARIHTLGGLATVHAEEVCGTAPETLAEHDRARPGEGEARAAGRVGELAPVGMQL
                     HFCHLSTAASVRAARGSVEVTPHHLFLSYEDFDPDNTLARVNPPLRDEATRRALWSCW
                     DAIDVIASDHAPHTLAEKSVPFRSAPSGIPGLETMVPLLMAAVYRGRISLLSVIEKTS
                     WRPSEILGIPRAGFEPGDRADYALYPDELTRIDASRLHTKCGWSPFEGCEAVFPEEVI
                     VGGVRVYAHGDLLEARPRFCPGKGYLLQ"
     gene            complement(8786..9700)
                     /locus_tag="PK196_00855"
     CDS             complement(8786..9700)
                     /locus_tag="PK196_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HON40006.1"
                     /translation="MLRFADFKPVSLEDREVFQEHYQHYPQMHSDNTFANMVCWNHYA
                     DYRFCKAGGSIILSSTIDGVTAFRCPIGPEDPDLLDDVIDLSLREGGETPLQVLDPAG
                     EAFIRKCYPDLPLHPDRNFFDYVYRTDSLADLPGKGYATIRHQVNRFARDYNYTIEEI
                     TRDNLDDVWEFLIIWCEWRDCDSMPILAYEKEAILFAVNNFFEIGLEGWAIRIGDAIG
                     AISIVGPVNRNMAVVHFEKALPETYRDIYKVIMSETARELRDRYRYINRECDMGAPGL
                     RESKTRYHPACMVEVHYTTRDDLEACCR"
     gene            complement(9704..10135)
                     /locus_tag="PK196_00860"
     CDS             complement(9704..10135)
                     /locus_tag="PK196_00860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON40007.1"
                     /translation="MTDEGEFEVRLVDAWDLDAIADLYRAGGWWKEEWNPAGLPSLIR
                     GSFAFAVAVESSTGRTVGMGRVISDGVSDGYVQDLVVHPNYRDRDIGTMILSALLKEC
                     TSAGVTWIALIAEPETEAFYARSGFQRMEGHTPMRWYPEKR"
     gene            complement(10201..11196)
                     /gene="hypE"
                     /locus_tag="PK196_00865"
     CDS             complement(10201..11196)
                     /gene="hypE"
                     /locus_tag="PK196_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HON40008.1"
                     /translation="MKVNLMHGAGGEVMGELLRVITNLEHNNAGGIGLESLDDGAVIP
                     LNGQNIVFTTDSHVVSPIFFPGGDIGRIAICGTVNDLAMMGGRPIALSCGMVIPEGFE
                     VADLERIVASMDAALGECGANLVTGDTKVVERSALDTIIINTAGIGIAGRVVRDCGLS
                     IGDLIIVSGTLGDHGIAIMAHREGFDFGGQIQSDVSPLWPLVERALAAGEIHAMKDPT
                     RGGFANAINEMARKSGVGVIIDEVNLPIRASVRSAAGMLGIDPLEVANEGKVVMGVAP
                     DDAETVLEALRSHPYGREAAIIGRVVEGSHVVMRTTVGGERFIEPPIGDPVPRVC"
     gene            complement(11193..11534)
                     /locus_tag="PK196_00870"
     CDS             complement(11193..11534)
                     /locus_tag="PK196_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HON40009.1"
                     /translation="MHEYSIAYDIYTTARRAAMENDAKEVKRITLNVGRMAMVNPEQV
                     EFLFNVIVEDDPLFPETELSCQDVEVRTRCSCGYEGDERFVCPRCGKLPEVVAGMEIV
                     VTRIEIEVDGE"
     gene            complement(11534..11758)
                     /locus_tag="PK196_00875"
     CDS             complement(11534..11758)
                     /locus_tag="PK196_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HON40010.1"
                     /translation="MCIAIPAEVIEIKEGNIGVVDFGDLKQEVRLDLVDVRVGEFVLV
                     HVGFAIQRLSREEGLETREVFKQVYAAMED"
     assembly_gap    11860..11959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12078..13316)
                     /locus_tag="PK196_00880"
     CDS             complement(12078..13316)
                     /locus_tag="PK196_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HON40011.1"
                     /translation="MGDFARQAPDKETGEDIYQYLLERINSLENRNLELREQFRQMES
                     EKRYIETQKIRYERELRKLKSEIEQLRSPPLVIGTVTDVIDNNRVIVRSSAGPRFLVR
                     TSQLIDPELLKPGVRCTLNQQSLAIVDVLPTSYDAQVYGMELVECPDETYEHIGGLEP
                     QIEELREAVELPLTKPHLFERVGISPPKGVLLYGPPGTGKTLLARAVAHHTNAHFLRV
                     VGSELVQKYIGEGARLVRELFDLAKQKAPSIIFIDEIDAIGAHRSDSTTSGDREVQRT
                     LMQLLAEMDGFDNRGDVKIIAATNRIDILDRALLRPGRFDRMIEIPLPDHQGRLAILK
                     IHTKHMNLGEDVDLTEVARLTEGKNGADLRAICMEAGMFAIRMERDIVTREDFMKAIE
                     KLSMDFDRHHIHTTFGEMFA"
     gene            13374..13865
                     /locus_tag="PK196_00885"
     CDS             13374..13865
                     /locus_tag="PK196_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HON40012.1"
                     /translation="MQCELCGAPIQGQSKIIQIEGAELRVCARCAKYGTEVQQPRQSG
                     GSQKKPGMVATQTPRRKPRDVFDLMEGELVEDYPNRIRAAREERGWSTRDLAQAIKER
                     EILIKKIEKGDLIPEDDVRQKLEKALNIRLIDSAEDSISIGGPDRVTTTVGDVIAFKR
                     SRK"
     gene            14170..14961
                     /locus_tag="PK196_00890"
     CDS             14170..14961
                     /locus_tag="PK196_00890"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HON40013.1"
                     /translation="MRGKEIRLERIMDRNTGRTIIVPLDHGVTLGPIPGLIDVEKTID
                     LVADGGANAVIGHLGLALHGHRRHGRDVGLIMHLSASTSIGPDPNDKVLVNTVTNALR
                     MGADGVSVHINVGADSEARMLQDLGRIAVECIEWGMPLLAMMYPRGRKIADEHDLESV
                     KLAARVAAELGADIVKTVYTGDPDSFREVTRGCPVPVVVAGGSKTDDRAILDLVEGAM
                     EGGAAGISIGRNAFQHPTPDRLIRAAALIVHEGRSAEEAIEILRM"
     gene            14967..15752
                     /locus_tag="PK196_00895"
     CDS             14967..15752
                     /locus_tag="PK196_00895"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HON40014.1"
                     /translation="MIGKEIRLERIMNRNTGRSVIIPMDHGFTMGQIEGLRNMPETVN
                     AVSEGGANAIVLHKGMVKGGHRKSGKDIGLIVHLSASTSLNPDPNDKVLVCSVEEAVA
                     LGADGISIHVNLGAPNESRMIESAGEVVRDCNRWGMPLLIMIYPRGKGIDPVSPQTVG
                     HCVRVAEELGADLIKTNYTGDPDSFRQITAACSVPVLIAGGEKGGDMETLMTIRDAVD
                     AGAAGVCMGRNAFQRDDPARFVAAVCRVVHEGVSPTEALETTQ"
     gene            15749..16735
                     /locus_tag="PK196_00900"
     CDS             15749..16735
                     /locus_tag="PK196_00900"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HON40015.1"
                     /translation="MKLFWVDVRPWRKNLATTAIESGADALLVDDAERVRELGRVTTI
                     APDGDLVPGKDLFEVEIVDKESEEEALRLSRQGQVIVRTQDWTVIPLENLVAQSERII
                     AAVSNAEEAKVALTVLERGAAGIMLSTDDPAEIRRVAEVIAGTTVPVPLVPFEVTRIT
                     PVGMGDRVCVDTCSILANGEGMLVGNTSSAFLLVHPETLENPYVAPRPFRVNAGAVHA
                     YTLLPDGKTAYLSDLSVGDRVLIVEHSGETHEAVVGRLKIERRPLLLVEARSGEAKVS
                     LVLQNAETIRLVGEGGAAVSVAALAVGDRVLGSLAEGGRHFGIAVRETILEK"
     gene            16740..17567
                     /locus_tag="PK196_00905"
     CDS             16740..17567
                     /locus_tag="PK196_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HON40016.1"
                     /translation="MIAGIIGGTGQMGRFFAGVFQTAGWETVVSGRRTELTNRDVAEM
                     ADLVLVSVPIRATAEVIREIAPLLSEDQVFCDLTSLKVEPVRAMLASRAEVIGLHPMF
                     GPGVASLRGQTIVATPARCSQETLDTLLSVFREQGAEITISTPEDHDRMMAVIQGLTH
                     FVTLAKAEAIRRTGADIAESLRFTSPIYRIEMGLVGRILAQDAGLYGDILQMNPAVPE
                     VLARFEEAVRALREIVESGDADRFQEFFAANAAHYASYLESATEETDDLINHVVSGR"
     gene            17564..18358
                     /locus_tag="PK196_00910"
     CDS             17564..18358
                     /locus_tag="PK196_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HON40017.1"
                     /translation="MTVLTLGPAGTVSHDLAARLYGDDIELLPTIRSIIARVASGSGR
                     GLVPIENSEAGGVGETLQGLMEFEVSITAEAYMPVRHHLAARYDPSRLSVIYAHPQTH
                     EQCSIFLDNLGVEVVHTSSNAASAVAMQRNENAGAIISEMAARIYSLPIAVRDVQNRR
                     DNTTRFVEISPLPRDLAGCTKCSLLVDPEIDRVGLLADILGVFARRGINLTRIESRPS
                     RRGIGKYIFFIDLEISEGWLEAEEELRGMTNLRELGCYPRLEVPGV"
     gene            18355..19623
                     /gene="aroA"
                     /locus_tag="PK196_00915"
     CDS             18355..19623
                     /gene="aroA"
                     /locus_tag="PK196_00915"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HON40018.1"
                     /translation="MIVRVSRTGPIKAVFEAPPSKSYTHRALIAGALAEGETRITNPL
                     RAVDTELTAGGLEALGVPVEWRPGGILVTGCAGTFRTGGEVTIDCGNSGTTLRLLASV
                     ALLAPHPVVLTGSSRMLERPIGPLARALEGLGGEVTFPGRPGYPPVRISGRLRGGRTE
                     IDGSVSSQFVSSILMAAPCAEEDVEVTLPAPPASRSYLDLTLDVMREFGARFERRGYE
                     WFRVESGVPYRGRDYRIEGDFSSASYLFAIAAICGGRVTVRGLNPASVQGDRRFLDAL
                     VSMGCRVASSDDAVTVERTGGLEGIEIDMSSSPDTVQTLAAVAAVAGSPTTITGTAHL
                     RYKESDRVAVTAETLRRMGAGVEVTADSIRISPAPLHGVAVDPHGDHRTAMAFAVLGL
                     AVGGMVIRDPGCVEKSFPGFWESLSGEGLL"
     gene            19620..20984
                     /gene="aroE"
                     /locus_tag="PK196_00920"
     CDS             19620..20984
                     /gene="aroE"
                     /locus_tag="PK196_00920"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HON40019.1"
                     /translation="MRVILIGYRGTGKTSVGRILAESLNLPFFDTDTLVERTAGVSIP
                     EIFRQHGEARFRALERGVIESLAGVEGVISTGGGAVCDPANVADLRRHGRVYLLSASP
                     ETIGERIAGSDRPGLTDLPPEEEVRSLLARRKDAYLGAADACVDTGSRTPVEVAGIIL
                     RDIRGENALPLEGRRERDGLLERFGLSSLREMLCRDPGLMVCGIAGYPCAHSRSPLLY
                     NRLFERFGMNYHYTRFEWPDVGTIIRLASLLPTKGLSVTIPFKTDVMRYLDEVDEHAA
                     AIGAANTVVRCGGRSYGHNTDWLGVRTPLLHRQGARAVVLGAGGAAAAAVYALCSLDM
                     EVTILARKPDAARRLAERFNCRWGGLEDFRGCKADVVLNATPVGMEPDTRSLLDAGDL
                     EQGMTVFDLVYTPPVTPLIRAAREAGCEAIPGTEMFVHQAVEQFRLITGIAVSPEEVR
                     EVLS"
     gene            20981..21976
                     /locus_tag="PK196_00925"
     CDS             20981..21976
                     /locus_tag="PK196_00925"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HON40020.1"
                     /translation="MNTFGRNFRCTTFGESHGRAVGVVIDGCPPGMPLTEGDIQPYLD
                     RRRPGKSPLESGRREPDRVEILSGTFEGRTTGAPIALIVLNQDVRSEDYDALRDVYRP
                     GHADYTWQAKYGLRDHRGGGRSSGRETVGRVAAGAVAIRWLAPRGIAIRGEVVEVHGV
                     RGREAMEAEIRAARDSGDSVGGIVEVIASGCPPGLGDPVFGKIDAAIAGAMMGIGAVK
                     AVEIGEGFGAARLLGSEMNDAITAEGFASNRAGGILGGITNGEDIIVRLAVKPTPSIR
                     SPQRTIDLAGVEREIRIEGRHDPCIAPRILPVAECMLALVIIDAMLEEAKYRGWD"
     gene            complement(22008..22637)
                     /locus_tag="PK196_00930"
     CDS             complement(22008..22637)
                     /locus_tag="PK196_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HON40021.1"
                     /translation="MKIVVSVDDPALISGVLEYNPAFIEIRLDRMEGDLLNHVRAIRE
                     MTAIPLIATLRSREEGGGFVGDADLWAGIVGPIAGYVDIVDVETRYSKHAPSLRSQGA
                     RILASLHTDDMPGQADLERIERTLRSYGDIPKIVVRPENLDELLDLLRFTANAQKPIC
                     TSVMGGRFRFARAILPLFGSEFVFCHAGRPMAEGQYHIREMREIAELLG"
     gene            22896..23459
                     /locus_tag="PK196_00935"
     CDS             22896..23459
                     /locus_tag="PK196_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON40022.1"
                     /translation="MEIPTPAELRAKRIQMGLKQADVARMAGISQSMVARIEAGSVDP
                     RVSTLAKIVNVLQAAEKSAVTAADVMHSPVFSVTPEDPVSRAIEIMGKKGISQLPVLE
                     NGVPIGCISESAITNAMEEGGLHQKCPRVVRDYMEPGFPTVPPTTPIDTVVHLLRHSH
                     AVIVIEKGKVQGVITRHDLISLITQVF"
     assembly_gap    23676..23775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23776..24440)
                     /gene="tpiA"
                     /locus_tag="PK196_00940"
     CDS             complement(<23776..24440)
                     /gene="tpiA"
                     /locus_tag="PK196_00940"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HON40023.1"
                     /translation="MDSPFILINLKTYKEGMGSNAHRIAAAAETVAKESGAVIGIAPT
                     FTELHPMSHHYSIPVYAQHIDPISPGAHTGHILPESVRSAGARGTLINHSERRLTLAD
                     IDACVQAARRLRLESVVCTNNDATSAAAAALAPDYVAIEPPELIGSGVSVSKADPGII
                     ERSVNAVRGVNPNVKVLTGAGIQSGECVKIAIDLGTCGVLLASSVVKAEDPEAVLRDL
                     VSLV"
     gene            complement(24538..27075)
                     /locus_tag="PK196_00945"
     CDS             complement(24538..27075)
                     /locus_tag="PK196_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HON40024.1"
                     /translation="MPYYHLRGGGLPELLAPAGSPEALAAAVSAGADAVYLAGRRFGA
                     RHYAANFSEEELVSAIDYAHLRGVRVYVTVNILVRDAELSDLARYLNRLYEIGADAVL
                     VQDPGAAALAREVVPDLPLHASTQMTIHNREGVLRAAGEGFSRVVLARELTIPEIEDI
                     AADPGARGVGLEVFAHGALCYSYSGQCLLSSVIGGRSGNRGMCAQPCRKPYRLVAAGV
                     DEYGRPGDLEPLPVNDRYLLSTRDLCVYPYLDRLVRAPVASLKIEGRMRSPAYVAIVT
                     RIYRRALDAIAAGNDWSPSREDLRDLALAFNREFTEGYILGARDIMARDRPGNRGLLL
                     GRVTGYDPRRGEATVRLAGDIVPRSGDGLVFCRDDPAMDVGAVVRRTSAVRNGTLRLS
                     VPAPVTPGARVFLTKSMDLEDGAKRIMEAPPEPLQLDLLVTWDGATPCLEAVVELPGG
                     KPLRVRYRPDLQMDAARNRPLTEEQIAAQFGKTGGTPFVIRSMDIRYPGGLFAPLGEL
                     NRVRREFLERVEEAVLAARRPDAEAVHAARQRLERALTGMEQAAALNRGRRVPSVSVY
                     TDALEGVEAAVSGGAGMVYLEVAAVPSLLKEAASVCRDGGAEVVWKWPAITRRSFLDA
                     ATPILPSLYGAGVSGVMVSGMGAADAVRRAEPRMNLYGAAGLNLWNHRTAARLDGLFL
                     RCTASPELPADDLAGLAASTGGTLELEVLVQGSIEAMVSEDRLVGGVAGEAGCSRGFI
                     GLQDGRNRVFPLRCDCEGRTCIANAVETCLIDHLPEIAEMGIDSVAIDARGRGPRYAG
                     EMARFYRAAVDAVARGDFGLLPGLRNEVKRRALGGITGGHFVRGIEG"
     gene            complement(27269..28156)
                     /locus_tag="PK196_00950"
     CDS             complement(27269..28156)
                     /locus_tag="PK196_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40025.1"
                     /translation="MITARNLVKYYGEFPALDGVSFDLDDGEIFGVIGHNGAGKTTLL
                     KIMAGLISPTAGSLVINGIDVVKRPLDLKRNLGYLPEESRLYETMSVEAYLAFFGEIY
                     GLPADVIRKRSRELLDELMLDPDGKKIGELSKGMKRKVAIARSLIHDPDLLIYDEPTS
                     GLDPMTSRSVLDYIKGLRNQGKTVVFSAHNLFQVEEACDLILILRRGKVVAKGSMHEL
                     REAFGSITYQIFFRIPDPATFSTPVSYIASNGRYMAEAHSIEDLNRMTATLAAAGATI
                     ERIESHYPTLEEMLLKIGQ"
     gene            28237..30042
                     /locus_tag="PK196_00955"
     CDS             28237..30042
                     /locus_tag="PK196_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HON40026.1"
                     /translation="MSLISSIRTIATWEIKRSMTTMGRGILPIAVGLLLLLVLVTAFA
                     AESGLHLQDGMYRIGVDDPGTASIVASDTRFATYLDDAASLWENRFSYDIVIINGEVY
                     AADTDKGRAALNTLERDYESYVASVAATEPDLFAAYPLWIDLQYVKSEIDFLATQSGQ
                     QVGAPVETRRPPSPSLPVEEVTPPSQGRLTTEEELREHLVEAGDGQPLKRYTSLISGE
                     SATDRLLTPSELSLPLPFDTIILVFVFIFPLYFTSQFFMMSVMNERVGRAGEALLSTP
                     LRPVAIVVGKALPYFTIMILIAAAITLFARAPLAILLPLVPVILFFLANALIIGMTSR
                     SFKELSFVSIFFSTVATSYLFFPTVFANTHVISIVSPLTLVVVAIQGDGFTAMEYIYS
                     TALFFATSIILFYVGIANFREEHLFSERRLTSRVVDFISTGISRNHPHFSLFLLAAFM
                     IPFVFMAEMLTLVLFFNIPMPLSLLLLTITAAVIEEFAKSIGLYAIVRDRPGFLTPGN
                     LLAGATVTGLGFLVGEKLLLFATLAQITESIFGSILFLSLQALWMPLLLHIAGVLITG
                     GFLLIGGRRAYAPGVIVAGIVHSLYNLYFIGGMLL"
     gene            30039..31112
                     /locus_tag="PK196_00960"
     CDS             30039..31112
                     /locus_tag="PK196_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40027.1"
                     /translation="MKRRHISLIAKKEIAGLSSEKTIVFAILLQVFIAMFSSFLMVGL
                     TAMYDPEAIEGYSTVTYRVGYAGAESPLIDEFGERDDLILYRMDLGEALAALRERMLS
                     AVVYVPETPPDAEGPITVTLYLIQNDLQSGVVNVKIKEVLQGYEEQLRETRADRLIYS
                     PVELNFPDTDGGESFFEFVYGLLIPLLVFLPAIVSAALIIDLITEEYQRQTLETLLST
                     PITFTEVIWGKIAACEVLVPLQAGAWLILLWLNGIPVGNAMAILLHVSTSGLILILLG
                     ALVALHYRERTSAQFIFSTALVVVFIFVMAVPHNPLNLIVRLAADSIGPVHWLVLALV
                     AAAAALLGWAITLYARRVGEKTP"
     gene            31197..32504
                     /locus_tag="PK196_00965"
     CDS             31197..32504
                     /locus_tag="PK196_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase glutamine
                     C-methyltransferase"
                     /protein_id="HON40028.1"
                     /translation="MEITILSPGIYTYGAMLIGGVLRDAGHRVTLTRTPEVPMGSILL
                     MSLFSTQHLLDPAIRDVIRAHRERGGRVYVGGPVSAAPEMVLGEIAPDAVVVGEGEET
                     VVRLVEEGVSPDLPGIAFLDDGRTVVTPPAPPAPIERPLPLIPDDIGSQSIRGASAYI
                     ETHRGCIGGCTFCQVPRFFGRRVRSRSIESIIEEVKAFAAHGAKRLSISGGTGSLYGS
                     SSGEMNPDAFIALLRGMAEVMGPKNISAPDIRVDCITDEILDAIKRYTIGWVFFGIES
                     GSDRLLRMMGKGVKVRQVVEAVERCRKHRLHVAGSFIVGYPGETERDYMATKDLIATL
                     LLDDTFVSAAEPIPGTPLADLVVKTPRDKNPTFMPHKSDYRALRLTESEARCLDLMMH
                     ADLFRKRIRIVNDEIYDAYLAEVRKQGKEIRAATDLILRYAGV"
     gene            complement(32566..33651)
                     /locus_tag="PK196_00970"
     CDS             complement(32566..33651)
                     /locus_tag="PK196_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HON40029.1"
                     /translation="MSIVEQKLSSLTAAGQFDICAPGECIHVLSTAAHITYNRRAGGC
                     GRMLKVLLNGYCSYDCAYCSVRLKRERLGFSPDEFAEAFLRLWHEDLVGGLFLSSGIP
                     HDVDLVMHDLVETGEILRCRGYDGYLHLKILPGAARADIHDAARVADRISINLETTSS
                     DRLGCIAGVKDYQQDILKRQAWVAEEKPEGHTTQLVIGAADETDAEILSCLAGQYRRV
                     RPARVYFSAFRSLPGTPLAEHPDTPAWRARRCYQADYLLRDYGFTADELKASLNEDGF
                     FENRDPKEVIAANMMPVNVNTAAKWDLLRVPGIGPKAADRIIALRKQRPFTGPADLAR
                     AGIRGQVAARHLTFGNRKETIQTKLIL"
     gene            33785..>34813
                     /gene="ablB"
                     /locus_tag="PK196_00975"
     CDS             33785..>34813
                     /gene="ablB"
                     /locus_tag="PK196_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HON40030.1"
                     /translation="MSGSRQDQKNQIRSSEAGAAFTRHTVESSRRQSVQGYSPCTDAC
                     DDTVTALGRSLVHHGPHNDRAFLYHLDPTDLPEITGRLGAFAKERGYSRIFARIPATA
                     LDQFVSGGYTPLVRIPGLFRGEVDGCYLAYRHPTRGSRRSGNDEVLAVAMAKAGERDA
                     ACPALEPCFTAVRATAVDAPAVASIYRSVFETYPVPIDDPGYLAQKMQENLRCFCIIE
                     PGGRIVAVAAAEVDARAEFAEMTGFATLPGYRGHGFAAILLQLMEDDVSSSGIKTVFA
                     IARARSYPANITFARAGYAFAGALSESVNICGSVEDMNVWWKSIGVLDRSRQTVRRGN
                     NIRFQGKPL"
BASE COUNT         6683 a        10238 c        10441 g         7251 t
ORIGIN      
        1 cccggcctcc ggtctggtgg acatggagct cctcgcccgt gccgccggga tccggaatac
       61 gtgcaaggtg caggacgagg aggaactcga ggaggcatgg gagagccggg gccggggacc
      121 gaacttcatc cacgttgttc tgaatcccgg gaacgcaccg gtcggaaaca tccctctcgc
      181 cccggccgag atccgggagc ggtttgtgcg ggcgcttggg aggtaggtat ccagctcgtt
      241 cctgtggcgg agtaagtaca ctctcttttt cccgctggtc atattgagta tcggaacttt
      301 tcccgtatcc tgtttctcat cgcgtccctt gagggttgcc agccggaaac ggttgcacac
      361 accgtcgcaa gaagaatgat ctcctgtgtt tttacctcag gaggaggatt tgttttcggc
      421 agttttggta acagccccag aacatgtaaa ggaatgggac agaatttata aataaactta
      481 cgtccatgtc cttgtggggg cgcctcgatg atgcgtctgc aaaagttcgt ggggtccccc
      541 catccacggt tgacgcccgg atgatagagc cccgcaagcc cggaggcaaa gggaacgtaa
      601 gcgttctgcg tacttaaacg tgcgcctctc ctgcctccgg gaaaaggttg gaggaaaacg
      661 acaatgaaca gaaacatcgg agcgcgggtg ctctctgtgc tcctggtgac ggctttcatg
      721 gggatggcat gcatggcccc ggtcagtgcc gggacgggcg acgattccga cgcgatctcc
      781 ccggaattcg gggtggagta caaacagaac tataacaccg ggaacaacct gccaaactca
      841 cccgccattg ccgaaggctt ctatgaagtg ttcgataatg ccgggtgggg gtgtaacatc
      901 tatatagggg actgggattc agatgcagcc catgtcgata gcgtagacct cgccttctgg
      961 gcaggacacg gtgacgacag ttgcatcctt ctggatggtc tcccgcttgt tctcgcctgg
     1021 gtatactttc atgactgtaa ttggggcgat caggatctgg aatggatctt gctgcattcg
     1081 tgccataccg ctcaagtccc cggagaattc aaagcgtggc cacattgggc tttgaatggt
     1141 gcccatctgg tttgcgggtt cgacacggtt ggctacgact acgcccagga tggggcggcc
     1201 gtcgcaaaca agctctttaa caattataag gtgcgtcacg cgtggtatca tgcgttggat
     1261 gagacccacc agagcaacgt ttggatcggt atcattggtg agaacgccgc ttgcggcgat
     1321 gaccgcatct ggggagaggg atctgtgatc accgatccac ctgttgacag tgagatctac
     1381 cggtgtttac tccagtgcaa ctaaatcgag gttttagtat ggaactaaaa aaaaaacaaa
     1441 atggccttcc cagcgctacg tctttgaccg gaagagagcg cgggccagcc gttcgacaaa
     1501 actctccttg acgcccgcag tttcctcttc gatgtactcg atgcctgcca catccgctgc
     1561 gatgcgtctg aaggcacggg atgcgctgga tgcgggatac ttaacaacga ccggtgtcct
     1621 tcccgcggac gcgcgtcgaa cgtttggatc ctcgggtatg accccgatca ccctggttcc
     1681 aagcagtttc tccatctggg ctgtgttgaa ctcgtttccg ctgcttgcga cccggttgat
     1741 gatcgccccc tctatatgcc cgccgacggt ctcggtcagg atctttgtct ttaaggaatc
     1801 gacaatcgag gatatctcgg ggtttaccac caggatcacg ccgtcggcga gagtcagcgg
     1861 gatgacaccg tccctgctga tccctgcagg agcatccagt atcagtatat cgaactcgct
     1921 cacgagatcc gtcatgacgt ctttaagccg atcggggttt gagtgctgga atccctgtag
     1981 agagagtccg gatgggacaa cctttacccc gaacggccct tcatagatgg catccctgac
     2041 acgcgcctta ccggccagca cctcgtgcag ggtcacgggc agttcgtcaa gcccgagaat
     2101 gagcccgagg ttagccatgc cgacatcggc atccagaatg caggtcttct taccgtaacg
     2161 ggcaagcatc gcccccagat ttgctgtgac cgtggtcttc cctgtaccgc ctttaccgga
     2221 agcaatcgtg tataccttta ccatgtaacc actcggtgtt taggtctggt tggtctcata
     2281 cccagtgctg cagggtgttc tgtatatcct tttcgagtgc gtctctctgg tcatcggaga
     2341 tgggtgccga agagcgggtg attgctatca ccgtctctcc acgatgagac atgccaagga
     2401 gttcgattcc attctcaccg cggagtttcc cctgcgtgta gaggtggctg taacgcgcgg
     2461 cttcgtcctc gctccccggt ttcgttgagg cgatcaggag gccgtcagcg gtagccaagg
     2521 ttatggattc aagactgtat ttcctgctga tcagaccaag agcctcttcc aggctcccca
     2581 tgacgccagg tttgaggggg ggttcgatgc tgggctcctt gctggccggg cttgcggtcc
     2641 ccgctggcgg caaaggcacc tgcggagggc ctgccatcct gagagcccga ctgatctccg
     2701 caaggtaata ggtaattgcg gcaaaacccg tcattgttat tatcgtgagt aggatcagaa
     2761 aaaactggat gataccggtt gaatccattc tgttatcagc ccctgaagtt aggtctcgtt
     2821 gtccttgatg agatgatcca ggtgaatctt cttgagcatt tccttgcagt tcgtcctgaa
     2881 gttgctgaca aggtgctcga tgtttatctc ttccatattc cggaggagtg tgtcaatctc
     2941 gtcaccttcc ggttgagagg tctcggcagc ttcctgcgcg ggtttggctc caggcatggg
     3001 aatgccatgc gaatcagcgg caggttcagg actgtgtgtg ctgtgcgcgg gagaactttt
     3061 tcccacgcca ggggcagtac ctgacctttc tggaacagta cctgctttcg aggtttttgc
     3121 aggtttaaca tctgtaactg gtttcacctc tctgtgaata ccactgcctg ctggttgttt
     3181 tccgggggcg gctggctttg ccacttctgg aacacctctt ttcgctggcg atttcccgga
     3241 ttttcctgtt gcgggcacct cgatcgcaca ctggcggtta aactcctggg agagtcgaac
     3301 ctgctcgggc gtcagaaggt tcaattcaac acctgcctcc tcctcgctcc cgagcacccc
     3361 atcaagtgcc tgacacccat tcagccctcc gtactcggca agcaccaccg aaccctcgtt
     3421 caataccagt gttgcgctct cgctgccaag gacaatcctg cagatcccgg tgaactttgc
     3481 tgctctcatc tcttcgatca gagcaggggt tgccgtggac ttgatgatct tgtggaaccg
     3541 gccgcgtggc agttgcattc aggcaaagct agatctgttg tagagcataa agtttgtcat
     3601 ttataccttt catgagaatg aggccatcaa accaatgcct cactggtttg atgggctacg
     3661 cacatgctac caggaaaaga tgaggagttt atatcacaga attgttgatc tgatggcatc
     3721 agaatagagg tgggcaaccc ttgcgcttca tctgatggta tacctactgc cttatagttc
     3781 cactacagtc gatccaatcc tccagtatag ccaggatctt ctcttcggca acccggatcc
     3841 ccaccctgac cgccggatca atctcccggc tgaatgcctg gatctcaccc acttcaatcc
     3901 cgatggtcac gatctccgcc gtatacccga gttcctttgc tatcgccacc gcgtccccga
     3961 tcccgatatc atgggatatg cgcgcgtgga accccccggt tggagggttt tcgaacgtta
     4021 ggacatcgcc tgcacagtca cctataccaa ccatagcatc gacgataaca accttatcgt
     4081 agccttccat aagcgcgatc aggccaaaac caccgctacc gccgtcaatc gcatcaacgc
     4141 caggaaatct cccctcaaag aggtgcatcg caaggatccc tgctccatca tttcccatga
     4201 tggggttgcc gcacccgatg atgcagacac ggttttcgtg catcttcctc ttacctattg
     4261 attatatctg ccggggtgca aaatggtaat gtatgacccc tgaccacgag gactcggttt
     4321 cgggaggggg tgatgaggcg catgatccac atcgtaccaa aaccgaccga cacgccggaa
     4381 gacaactcga ccggcgccgt tatacgggaa ctggagcggg caggcgcccg ctaccgtctc
     4441 cttgacctcg ctgcaatcga cccccttacg tcggggcttg agcacgaaca catatgggtc
     4501 tgcggtatac ggcaggacct gcaccagttt gagacgctga atgtgctttc gctctctaac
     4561 cggattatca acactcccgc agccatcgct acctgtgcct ccaaggtgat gacgacggca
     4621 cttctcctgc aaagaggggt gcggacaccg gcgacagtct acgtctcttc agaagaacag
     4681 gcgcgtgact ttcttatgcg gcatggaaag gttgtctata agcctgtcta cgggtatgat
     4741 gggaatggca tccggcttgt gacagagccc ggcgacctcg ggcccggccc ctggtatctt
     4801 caggaatacg tcccgaacga tcgggacttc cgtgtcttcg tcctgggtgg agaggcggtt
     4861 ggcgccataa ccagggtctc tcgggggttg atgcacaaca tccatcaggg cgggatcggt
     4921 atgccggtcg agatcgatga cgatatgcgc gccatagctg aggcgtctgc ggcagtggtc
     4981 ggcgtcgatt actgcggtgt cgaccttctg tgcgatcggg aaggatacac agttcttgag
     5041 gtgaacggaa ctccaaactg gcactgtatg gcggcaccta tcccgaaact gcttgcctca
     5101 tacctcatcg agtgcgaacg cacgatgcgt tcctgaagag aaaggcgcgg ttggtgatac
     5161 cgggctgccc gcaccctgta tctcttactg gagatattcg aggtctttta aggttttatc
     5221 ggcgagttct ttcatcctgg cagaaatcct gcttgatgac gcgaattcca cgtccttcat
     5281 ggcgtttgca agttcagttc caagcacgaa gatagcgtac ttgtgttcca ttttgctctt
     5341 atgcacctga gaagggtcaa tctttaacgc gtagtattgt gaaaatttca gatccggatt
     5401 ctgcaactca aaaaaatctt taatctctgt gagaatacca tgtagcgcta ttaactcttc
     5461 cttgtgcatc atcctctccc cgagagaaaa agtggtggac gtgaatatat ataaatttgc
     5521 accggggatc ggcatcaaac tatctttatg agtcggatcg agagtgggcg ttttataatt
     5581 cctttaaaat ctgtggcagc cacgaactca aggtcctttc caccgagttg atcgaggagc
     5641 ttctggtcga tcacacggtc aacgacaagc ccttcaacat cgccgttcag gttctggagt
     5701 gcgttctcga catctgcggc gctggattcc aggaggacgg tgtaatcggg ggagagaaac
     5761 ctggcaatct tcttgttccg gacgtctgcc atgtgttcac caagtgtcga gggtgacttt
     5821 ttgccccccc cacccggcgg tggctgatca tcctcgccgc ccggagatct gacagccgct
     5881 ggtgtcgcat gaaccggcaa ccgctctgta gcctcctcca gagggatgtg ctcgatcagg
     5941 ccgaccgcag gcactttgtt gcggagcgct ttaacgatct ctttgcggct catctcctcg
     6001 acgctctttc cgcgcgggca gtaggccacg aaatcaatct cagcgacctg gaggagttca
     6061 cgaaggatca ggtcgccgcc ccggtcgccg tcgagcagcg tggtcgcagt cttctgtgtg
     6121 cagagatcga ttataatctg agggatgttt gtaccctcga ctgctacggc gttcttgatc
     6181 ccgtgacgca ggaggttgat gacatcggca cgcccctcga caatgattat ggcatcagag
     6241 accatgacat gagggccggc atggactctc tcttccccaa ggtactcaag tttctcgatt
     6301 cggatggctt ccctgacctc gtcgagcagt tcatcgctgt ttatggaccc ctcatcgaaa
     6361 ttttcaagta ggatctcttt tgcgcgctcg acgatccggc gtcgtttggt cacccggata
     6421 tcctcgatgc ggtcaaccat cacgtgggcc gtgcacggcc caacccggtc gatcgtctca
     6481 agcgacgagg ccaggagcgc ggtctctgcc ctgtcaagag atgacgagat gagaatctca
     6541 cccttggtct caccgcgttt tgtggttatc tgcacgtcta tcctgccgac acggccggtt
     6601 ctctgcaaat cgcgcaggtc aaggtcttcg ccgagcaaac cctcggtctg gccgaagatg
     6661 gcgcccacta catcgggttt atcgaccacc ccctcgatct gcagcgtaag gtggatgagg
     6721 tattttgttg tatcaggtga atacatggag tcctgcctcc tctgtaaatg taatccatat
     6781 ttctgtattc tccacgggtt tttgaatgcg cggacataca gataaatata tatttaagct
     6841 atgattactt aaatcagatg gaagtctcac agcaaccttt ttaaggacat aatctgaacc
     6901 tggaaaaggc ttcatcacat attttactta tatatccctc tcctttttgc tgtcttctgg
     6961 ctttcgtaat gagaattgcc ctaaaattgc aggttatgtt tctgacagac cattcatgcg
     7021 tcggagcggt tcaggcattc gagagcacgg gaacgtgata aaccggagac cgccacaacc
     7081 tttgatgaag atgcatgccc ggcaacgatc gtcacgttgc cgcgggacac ctcaaacgtc
     7141 tttgccagga ggtctatgac ggcacggttt gctctcccgc cgactgcggg ggctgccacc
     7201 tgacatcgga tactccgacg ccactcgttg taacccgcgg ggaacagaga ccgttttgca
     7261 cctgctgtaa cgtcgagagc aatggtcacc ccatccggtg tctcagagac agcattggca
     7321 taggtatcca tcagaaaagc cccttgaacg agtgtcgccc ggcactaacg atccaggatc
     7381 actggtggcc ttgtgccgtc cctcacccga tcgaacccat atgccgggcg tgcctgtcgc
     7441 gcacccgggt tgtcccgtgg ttcatatcct ggcgtcctgc ctgtgatcac ccggggacct
     7501 ataaggtgcg ctttatcggc actctattat ttatctcact gcaataaata tcctttgccg
     7561 gggcagaatc gggggcgtgc ctccaggagg tcgccgtggg cgtagacccg cactcctccc
     7621 acgatcacct cttccggaaa gaccgcttca cacccttcaa acggcgacca gccgcacttc
     7681 gtgtggaggc gggaggcatc gatccgggtg agttcgtcag ggtagagggc atagtccgcc
     7741 ctgtcgcccg gttcaaaccc tgcgcgaggg atgccaagga tctccgaggg tctccacgag
     7801 gtcttctcga tcactgacag gaggctgatc cgcccccggt acaccgccgc catcaggagg
     7861 gggaccatcg tctcgagacc ggggatgcct gaaggggctg atctgaacgg gacgctcttc
     7921 tcggcaagag tgtgtggggc gtggtcggag gcaatgacgt cgatcgcatc ccagcatgac
     7981 catagagcac gccgtgtcgc ttcgtccctt agcggcgggt tcaccctggc cagggtgttg
     8041 tccggatcga agtcttcgta cgagagaaag aggtggtggg gtgtcacctc tacgctgccg
     8101 cgggcagccc tgaccgaggc agcggtgctc aggtggcaga aatgaagctg catccccaca
     8161 ggtgcgagtt cgccaaccct ccctgcagcg cgtgcctcgc cctcacccgg ccgcgcacgg
     8221 tcgtgctcgg cgagggtttc cggcgccgtc ccgcagacct cctcggcatg gacggttgca
     8281 agccctccga gggtgtggat acgggcgaag agagacctca ggctctccag gtcaagcccc
     8341 tccccataac tcgacggtgc tgcgaaagtc tccccgaacg ccatcgcgcc tgccttccac
     8401 atccctgtca ggtccgcgtc cgggaggaca ccagcgttaa tggcaaaccc gcatacggcc
     8461 tgttcccctg cctcgcggat gcgcgcccgg aggcggtcgg gtgtggtgat cggggggact
     8521 gtgtttggct ggtcgacgac gagcgttacc ccgccggcga ccgcgcttac cgtgcccgaa
     8581 cgccagtctt ccttatctgc ctgcaccccg ccccgcatgt ggacatgcat atcgaccgcg
     8641 ccgggaaggc aggtgaacct gctgcagtct atcgtctcat cggcacgaac cgctgcgccg
     8701 acgtggcgga caacaccacc agtgacggcg atatcggcgc gccttcctgt aggtagtgca
     8761 acgttccgga gcagcaggtc ggcggtcacc ggcagcatgc ctccaggtcg tcccgggtcg
     8821 tatagtggac ttcaaccatg caggcggggt ggtagcgggt ctttgactcg cgaagccctg
     8881 gcgcacccat gtcgcactcc cggttgatgt agcggtagcg gtcacgaagc tctctcgccg
     8941 tctcgctcat gatgaccttg tagatgtcgc gatatgtctc tggaagagct ttttcaaagt
     9001 ggacgacggc catattcctg tttactggcc ccacgattga tatcgccccg atggcatccc
     9061 caatccttat agcccagccc tcaagcccga tctcgaagaa gttgttcacg gcaaacagga
     9121 tggcctcttt ctcgtaagca aggatgggca tggagtcgca gtctcgccac tcgcaccaga
     9181 ttatcaggaa ctcccagacg tcgtccaggt tatccctggt gatctcctcg atggtatagt
     9241 tgtagtcccg cgcaaaccgg ttcacctggt gccgtatggt ggcgtaaccc ttccccggga
     9301 gatcggcaag gctgtccgtc cggtagacgt agtcaaagaa gttcctgtcc gggtgcagcg
     9361 ggaggtcggg gtaacatttc cggatgaatg cctcgcccgc cggatccaga acctgcagag
     9421 gcgtctcccc gccctcccgg agtgaaaggt cgatgacgtc atcgagaagg tcgggatctt
     9481 ccgggccgat cgggcagcgg aaggcggtta caccgtcgat ggtgcttgag aggatgatcg
     9541 aaccgccggc cttgcaaaaa cggtaatccg catagtggtt ccagcagacc atgtttgcga
     9601 acgtgttgtc gctgtgcatc tgggggtaat gctggtagtg ctcctggaac acctcgcgat
     9661 cttccaggct tacgggcttg aaatcggcaa atcttaacat gtctcatctc ttttctgggt
     9721 accatcgcat gggtgtgtgc ccttccattc tctgaaagcc ggacctggca taaaatgcct
     9781 ctgtttcggg ctcggctata agggcgatcc aggtgacacc tgcagacgta cactctttga
     9841 gcagcgcaga taagatcatc gttccgatat cccgatcccg gtagtttggg tggacgacca
     9901 gatcctggac gtagccgtcc gataccccat ccgagatcac ccttcccatg cctaccgttc
     9961 tgccggtcga ggactcaact gcgacggcaa aggcgaaact tcctctgatg agtgagggca
    10021 gacctgccgg gttccattct tctttccacc agccgccagc ccggtagagg tctgcgatcg
    10081 catccaggtc ccaggcgtcc accagacgca cctcgaactc cccttcatcc gtcatggtct
    10141 ctcttcatca gagcctgtct ggagatgcat tgaaatataa aagaaggtgt ggccgcccct
    10201 ctaacagacc cgtggtaccg ggtcaccgat cggcggttcg atgaaccgct ccccccctac
    10261 ggtggttcgc atgacaacat gcgacccctc aacgaccctg ccgatgatcg ccgcctcacg
    10321 cccatagggg tgcgagcgca gcgcctcgag aactgtctcc gcatcatcgg gcgcgactcc
    10381 catgacgacc tttccctcgt tcgccacctc gagcggatcg ataccgagca tacccgcagc
    10441 gctccggacg ctcgcccgga tggggaggtt cacctcatct attatgaccc cgacaccgct
    10501 cttccttgcc atctcgttga ttgcgttggc aaacccccct ctggtcgggt ctttcatggc
    10561 gtgaatttcg ccggcagcaa gcgcccgctc gacaagcggc catagggggg atacgtcgga
    10621 ctggatctgg ccgccgaaat cgaacccttc tcgatgggcc atgatggcga taccatggtc
    10681 gccgagggtg ccgctgacga tgatcagatc cccgatcgaa agcccgcagt cccgcaccac
    10741 ccgcccggct atgccgatgc cggcggtgtt tatgattatg gtatcaagcg cgctccgctc
    10801 caccaccttg gtgtctccgg tgacaaggtt cgccccgcat tccccaagtg cggcgtccat
    10861 ggaggcgacg atccgttcga ggtctgcaac ctcgaaacct tcggggatga ccataccgca
    10921 cgagagtgcg atcggccgcc cccccatcat ggcaaggtca tttactgttc cgcagatggc
    10981 gatgcgaccg atgtccccgc ccgggaagaa gatcggcgag acaacgtggc tgtcggtcgt
    11041 aaaaactatg ttctggccgt ttagcgggat gaccgcgcca tcatccagcg attcaagccc
    11101 tatcccgccg gcgttgttgt gctccagatt ggtgatgacg cgcaggagtt ctcccatcac
    11161 ctcgccgccg gcaccgtgca tgagattgac tttcattctc cgtccacttc tatctcgatc
    11221 ctggtgacca caatctccat tcctgcaacg acctcaggga gcttgccgca gcgggggcag
    11281 acaaaccgtt cgtctccctc gtagccgcag gagcagcggg tgcgcacctc gacgtcctgg
    11341 caggagagtt cggtctccgg gaagaggggg tcgtcctcga cgatgacgtt gaagagaaac
    11401 tcaacctgtt ctggattcac catcgccatc ctgccgacgt tcagtgtgat gcgcttcacc
    11461 tcttttgcgt cgttctccat cgcagcccgg cgggcggttg tgtagatgtc ataggcgatg
    11521 ctgtactcgt gcatcagtcc tccatcgcag cgtagacctg cttgaagacc tcacgggtct
    11581 caagcccctc ctctctgctg aggcgttgga tggcaaaccc aacatggacg agcacaaact
    11641 caccgaccct gacgtcgaca aggtcaagcc ggacttcctg tttcaggtcg ccgaagtcga
    11701 cgacgccgat gttgccctct tttatctcga ttacctcagc gggtattgca atacacatgg
    11761 cctctctcct tctcatgtta gtttagtctg cgtctccaaa aaaggttgac cctgctgcag
    11821 gagagaggcg ggagatgccg tttgagcggt ggaacccctn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnna tccccctgaa tagacctcta tggacgcccc tcgaatggaa
    12001 tccgtccccg tttttttgca gacctttccg gcataaagtt gatttcagtg atggtgaaca
    12061 cgtataaaaa gtttttatca ggcgaacatc tcgccgaagg ttgtgtgtat gtggtggcgg
    12121 tcaaagtcca tcgagagttt ctcgatcgct ttcatgaaat cctccctggt gacgatgtcg
    12181 cgctccatcc tgatagcaaa catgcctgct tccatgcata tcgcccttag atcggcaccg
    12241 ttcttgccct ctgttagcct ggcaacctcg gttaggtcga cgtcttcgcc gaggttcatg
    12301 tgcttcgtgt gaatcttcag gatggcaagc ctcccctggt ggtcagggag cgggatctca
    12361 atcatccggt cgaaccgccc cggtcgtaga agggcgcggt caaggatatc gatccggttg
    12421 gttgccgcga tgatcttgac gtcaccccgg ttatcgaagc catccatctc ggccaggagc
    12481 tgcatcagcg tccgctgcac ttctcgatcg ccggaggtgg tcgagtcgct ccggtgcgcg
    12541 ccgatcgcat cgatctcgtc gataaagatg attgagggcg ctttctgttt tgcaaggtcg
    12601 aagagttccc tgaccaggcg ggcgccctca ccgatgtatt tctgaaccag ttctgagccg
    12661 acaacccgga gaaagtgcgc gtttgtgtga tgggccaccg cgcgtgcgag gagggttttt
    12721 cccgtcccgg gcggaccgta gaggagcaca ccttttggcg gggagatccc gaccctctca
    12781 aataggtggg gttttgtgag agggagttct acggcttccc ggagttcttc gatctgcggt
    12841 tcaagacctc cgatgtgctc gtaggtctcg tccgggcact cgacaagttc cataccatag
    12901 acctgtgcgt cataactcgt ggggagcacg tcgacgatcg caagggactg ctggttgagt
    12961 gtgcaccgca ccccgggctt gaggagttcg gggtctatga gctgggaggt gcggaccaga
    13021 aagcgcggcc ctgcactgct gcgcacaatc acgcggttat tgtcgataac gtcagtaact
    13081 gtacctataa caagaggggg gcttctgagc tgctcgatct cgctcttcaa tttgcgcagc
    13141 tcacgctcat aacggatctt ctgagtctcg atgtagcgct tctccgactc catctggcgg
    13201 aactgctcgc gcagttccag gttccggttt tcgaggctgt tgatccgctc caggaggtac
    13261 tggtagatgt cttcaccggt ttccttgtct ggtgcctgcc gggcaaaatc tcccatgcgt
    13321 cccctcgatt acttatatag gttaaaataa atataaagtt gtttgcgaga gttatgcagt
    13381 gtgaactatg cggcgcccct atacagggcc agtcgaaaat aatccagatc gaaggagcag
    13441 aactccgcgt ctgcgcccga tgtgcaaaat acggtacaga agtccagcag ccgcgccaga
    13501 gtggcggttc gcagaagaag cctggaatgg ttgctacaca gaccccccga aggaagccca
    13561 gagatgtctt tgacctgatg gaaggcgaac tggtcgagga ttacccaaac cggatccgcg
    13621 cagcccgtga agagagaggc tggtcaacgc gtgaccttgc gcaggcgatc aaggagcgcg
    13681 agatcctgat caagaagatc gagaaaggtg acctcatccc cgaggatgat gtccggcaaa
    13741 aacttgagaa ggccctcaat atcaggttga tagactcggc tgaagattcc atatccatag
    13801 gggggcctga ccgggtgaca acgaccgtcg gagatgttat agccttcaag aggagccgaa
    13861 aataagtcat tcccgcattt ttagcgcggg agggtgctcc aaactctcgc ccgagtggat
    13921 accggcatgt ttttattcac aaacagataa tgctacactc aatgcgtggt gaatgttttt
    13981 tcgccggtga ccgtttttac cccggttaac ggagtagcca tcacgcacgc aggagttatt
    14041 tgccagattt tgaccgctga tatccgcaac tgcagagcgg cagcgttcta tcgctatttt
    14101 aactggttta gccagttttg atctttcccg gaaaagaccg tatcatcaaa ttccgggagg
    14161 agaatggtta tgagaggtaa agagattcgt ctggaacgta ttatggatcg aaacaccggc
    14221 aggaccatca tcgtgccgct cgaccacggt gtgacgctcg gccccatccc cgggctgatc
    14281 gatgttgaga agaccatcga tctggttgcc gatggtggag caaacgctgt gatcggacac
    14341 ctggggcttg ctctgcatgg tcaccggcgg cacggccgtg atgtcggtct gatcatgcat
    14401 ctatccgcga gcaccagcat aggtccggac ccgaacgata aagttctcgt caacaccgtc
    14461 acaaacgccc tgaggatggg tgctgacggc gtctcggtgc acataaacgt cggtgcagac
    14521 tcagaagcgc ggatgctcca ggatctgggc aggatcgcgg ttgaatgtat agagtggggt
    14581 atgccgctcc ttgcgatgat gtacccgagg ggcaggaaga tcgcggatga gcacgacctc
    14641 gagagcgtca aactcgcagc ccgcgtggcg gcggaactgg gtgctgatat agtaaagacc
    14701 gtctacaccg gggatccgga ctcgttcagg gaggtgacac gggggtgccc ggtgccggtc
    14761 gtggtagcag ggggttcaaa gaccgacgat cgtgcgatcc tcgatctggt ggaaggcgcg
    14821 atggaaggag gagctgccgg gatatcgatc ggacgaaacg ccttccagca cccgacgcct
    14881 gaccgtctca tccgcgccgc cgcgctgatc gtccatgaag ggcggtctgc agaggaagca
    14941 atagagattc taaggatgtg ataacaatga ttggaaaaga gatccgtctt gagcgaatta
    15001 tgaaccggaa cacggggcga tccgtgatca tccccatgga tcacgggttc acgatggggc
    15061 agatcgaggg gctccgcaac atgccagaga ccgtcaacgc ggtaagcgaa gggggtgcaa
    15121 acgccattgt cctccacaaa ggcatggtga agggagggca ccgaaagagc ggaaaggata
    15181 tcgggctcat cgtccacctc tccgcgagca cctcgctgaa cccggaccca aacgacaagg
    15241 tccttgtctg cagcgttgag gaagcggttg ccctaggcgc cgacggtata tcgatccacg
    15301 tcaaccttgg cgcgccgaac gaatcgcgga tgatcgagtc ggccggcgag gttgtgcggg
    15361 actgcaaccg ctggggtatg ccgctcctga tcatgatcta cccccgtggc aaggggatcg
    15421 atcctgtatc accacagacg gtcgggcact gcgtccgggt tgccgaggaa cttggcgccg
    15481 atctgatcaa gactaactac accggcgacc cggactcgtt ccgtcagatc accgccgcct
    15541 gttcggtgcc cgtcctgatc gccggaggcg agaagggtgg cgatatggag acgcttatga
    15601 ccatccgtga tgctgttgat gcgggcgctg cgggtgtctg catgggaagg aacgccttcc
    15661 agcgcgacga tcccgcccgg ttcgtggccg cggtctgccg ggtggtgcat gagggtgtaa
    15721 gccccaccga ggccctggag acgacgcaat gaaattgttc tgggttgatg tccggccgtg
    15781 gaggaagaac ctcgcgacca ctgcgatcga gagcggggcc gacgccctgc ttgtcgatga
    15841 cgcagaacgt gttcgggaac tcgggcgggt gacgacgatc gcaccagacg gtgacctggt
    15901 tccggggaaa gatctcttcg aggttgagat cgttgataaa gagtccgaag aggaggcgct
    15961 ccggctttcc cggcagggcc aggtgatcgt caggacacag gactggacgg tgatccccct
    16021 cgagaacctg gtagcgcagt cagagaggat catcgctgca gtctcgaacg ccgaggaggc
    16081 gaaggtcgct ctgaccgttc tcgagcgtgg tgcggcgggg atcatgcttt caaccgatga
    16141 cccggcggag atccgtcggg tggcggaggt gatcgccggt acaacagtgc ccgtccctct
    16201 tgtcccgttc gaggtgaccc ggatcacccc tgtcgggatg ggcgaccggg tctgtgtgga
    16261 tacatgttca atccttgcaa atggggaggg gatgctcgtc ggcaacacct cgtcggcatt
    16321 cctcctggtg caccccgaga cactggaaaa cccatatgtg gcgccccgcc cgttccgggt
    16381 gaacgccgga gcggtgcacg cctacaccct cctccctgac gggaagaccg catacctctc
    16441 agacctctcg gtgggtgacc gggtgctgat cgtggagcac tcgggcgaga cccacgaagc
    16501 ggtcgtcggc aggctgaaga tcgaacgccg ccctctcctc ctggtggagg cgaggtcagg
    16561 ggaggcgaag gtgagtctgg tgctccagaa cgccgagacg atcaggcttg ttggggaggg
    16621 tggtgcggct gtatcggtcg ccgccctggc ggtgggcgac cgggttctcg gcagtctggc
    16681 ggagggcggt cgacacttcg ggattgctgt cagagagacg atcctggaga agtaaaggta
    16741 tgattgcagg tatcatcggt ggcacgggtc agatgggacg gttctttgcc ggtgtcttcc
    16801 agacggcggg ctgggagacg gtcgtctccg ggaggaggac cgagctcacg aaccgggatg
    16861 ttgcggagat ggccgacctt gtgctggtct cggtgccgat ccgcgccacc gcggaagtta
    16921 tacgcgagat cgcgccgctg ctctcggaag accaggtctt ctgcgatctc acctccctca
    16981 aggtcgaacc cgtccgggca atgcttgcct cccgtgcgga ggtgatcggg cttcacccga
    17041 tgttcgggcc gggcgtcgcg tcgctgcggg ggcagacgat cgtcgcaacc ccggcgcgct
    17101 gcagccagga gaccctcgat acccttctct ccgtattccg tgagcagggg gcggagatca
    17161 ccatctcgac accggaggat cacgaccgga tgatggcggt gatccagggt ctcacccact
    17221 ttgtgacgct tgcaaaggca gaggccattc gcaggacagg agcagacatc gcggagtctc
    17281 tccgtttcac aagccccata tacaggatcg agatgggact tgtcgggcgg atccttgctc
    17341 aggacgccgg actctacggg gatatcctcc agatgaaccc ggcagtaccg gaggtgcttg
    17401 ccaggttcga ggaggcagtc cgggcgctcc gcgagatcgt cgagtcgggg gatgccgacc
    17461 ggttccagga gttctttgcc gccaatgccg cgcattacgc ctcctacctc gagtccgcga
    17521 ccgaagagac cgacgacctg atcaaccacg tggtgagcgg ccgatgaccg tcctgacgct
    17581 cggcccggcc gggacggtca gccacgacct tgcagcacgg ctatacggcg atgatatcga
    17641 actcctgccg acgatccgct cgatcatagc cagggtggcg agcggttctg gccgcgggct
    17701 tgtgccgatc gagaactcgg aagccggagg tgtgggagag acgctccagg ggttgatgga
    17761 gtttgaggtc tctatcaccg ccgaggccta catgcccgtt cgccaccacc ttgcggcccg
    17821 gtacgacccc tcccgcctct cggtcatcta cgcccaccca cagacacacg agcagtgctc
    17881 catcttcctc gacaatctcg gggtggaggt cgtccacacc agcagcaacg ctgcgagcgc
    17941 ggttgctatg cagcgaaacg agaacgcagg cgccatcatt tcggagatgg cggcacggat
    18001 ctacagcctc ccgatcgccg tgagggacgt tcagaacagg agggacaaca ccacccggtt
    18061 tgtcgagatc tcccctctcc cccgcgatct tgcaggatgc acaaagtgca gtctcctggt
    18121 tgacccggag atcgaccggg ttggactcct tgccgatatc ctgggggttt ttgccaggcg
    18181 cgggatcaac ctgacccgga tagagtcgcg gccgtcccgg agggggatcg ggaagtacat
    18241 cttcttcatc gaccttgaga tctccgaagg ctggcttgag gcagaggagg aactcagagg
    18301 catgacaaac ttgagagaac tcgggtgcta tccccgcctg gaggtgccgg gggtatgatt
    18361 gtcagggttt cgcggacagg ccccatcaaa gcggtctttg aggccccgcc gtcgaagagt
    18421 tacacccaca gggcgctgat tgcgggagcg ctcgcggagg gagagacacg catcaccaac
    18481 cccctccggg cggtcgacac ggaactgacc gccggggggc ttgaggctct gggggtgccg
    18541 gtcgagtggc ggcccggagg gattcttgtc accggctgcg ccggcacctt ccggacaggg
    18601 ggtgaggtga ccatcgactg cggaaactcg gggacgacac tgcgattgct tgcctccgtg
    18661 gcgctccttg ccccccatcc ggtggtgctg accgggagtt ccaggatgct tgagcggcct
    18721 atcggcccac ttgccagggc gctggagggg ctaggtggcg aggtcacttt tccaggcagg
    18781 cctggctacc cgccggtccg gatatccggg agacttcgcg gcgggaggac ggagatcgac
    18841 gggagcgtga gcagccagtt cgtctcgtcc atcctgatgg cagcgccctg cgcggaggag
    18901 gatgtggagg tcacgctccc ggcgccacct gcctcgcgat cgtacctgga tctgacactg
    18961 gatgtgatgc gggagttcgg cgcccgattt gaacggcggg gctacgaatg gttccgtgtg
    19021 gagagcggcg tgccttaccg ggggagggat taccggatcg agggcgattt ctcatcggcg
    19081 tcatacctct ttgcgatcgc ggccatatgc ggcggccggg tcacggtcag gggactgaac
    19141 cccgcatccg ttcagggtga ccgccggttc ctggacgccc tcgtctcgat gggctgccgg
    19201 gttgcctcca gtgatgacgc ggtgacggtc gagcggacgg gcggtcttga aggtatcgag
    19261 atcgacatgt cctcctcccc cgataccgtc cagacgctgg cagcagtggc ggcggtggca
    19321 gggtcgccga cgacgatcac cgggacggcg cacctgcgct acaaggagag cgaccgagta
    19381 gcggtgacgg cggagaccct gcgcaggatg ggtgcggggg tcgaggtgac ggcggactcc
    19441 atcaggatca gccctgcgcc gcttcacggc gttgctgtcg acccccacgg cgaccaccgg
    19501 acggcgatgg cgtttgccgt cctcggtctt gcggtcggcg ggatggttat ccgggacccg
    19561 ggatgcgtgg agaagtcttt tccggggttc tgggagtctc tctccgggga ggggctgctg
    19621 tgagggtcat actcatcggt taccggggga cagggaagac ctcggtcggg cggatccttg
    19681 ctgaaagcct gaatttgccc ttctttgata ccgatacgct tgtcgagcga acggccgggg
    19741 tctcgatccc ggagatcttc cggcagcacg gcgaggcgcg gttccgcgcc ctcgaacgcg
    19801 gggttatcga gtcgcttgcg ggtgtggagg gggtgatcag caccggcggg ggtgcggtct
    19861 gcgaccccgc aaacgtcgcc gacctgcggc ggcatgggag ggtctacctc ctctccgcct
    19921 cgccggagac cataggggag cggatcgccg ggagcgaccg cccggggctg accgatctcc
    19981 cgccggagga ggaggtgcgc agcctccttg ccaggcgaaa ggacgcctac cttggcgccg
    20041 cagatgcctg tgtggatacc ggttcccgaa cgccggttga ggttgccggt atcatcctca
    20101 gggatatccg cggtgagaat gctctcccgc tggaagggcg gcgcgagcgc gacggcctcc
    20161 tggagcggtt cgggctctcc agtctgcggg agatgctttg ccgcgacccg gggctgatgg
    20221 tctgcggcat cgccggttac ccctgcgccc acagcaggag cccgctcctc tacaaccggc
    20281 tctttgaaag gttcgggatg aactaccact acacccggtt cgagtggccg gacgtcggga
    20341 cgatcatccg tctggcatcc ctgctgccga caaaggggct ctctgtcacc atcccgttca
    20401 agaccgatgt gatgcgttac ctggacgagg tggacgagca tgcggcggca ataggtgcag
    20461 caaacaccgt ggtcaggtgc ggtggcaggt cttacgggca taacaccgac tggctgggtg
    20521 tccggacgcc gcttctccac cggcagggcg ccagggcggt tgtgctcggt gcggggggtg
    20581 cggcggccgc ggcggtctac gccctctgtt cgctcgatat ggaggtgacg atacttgcca
    20641 ggaagccaga tgctgcacgg agacttgcag aacgtttcaa ctgccggtgg ggggggctgg
    20701 aagatttcag ggggtgcaag gccgatgtgg tgctgaacgc aaccccggtc gggatggagc
    20761 cggacacccg gagcctgctt gacgccggcg acctggagca ggggatgacg gtctttgatc
    20821 tggtctacac cccgccggtt acgccgctca tccgggcggc gagggaggca gggtgcgagg
    20881 ccatacccgg gacggagatg ttcgtccacc aggcggtgga acagttccgg ctgatcaccg
    20941 ggatcgcggt ctcgccggaa gaggttcggg aggtgctttc atgaacacgt tcggcagaaa
    21001 tttcaggtgc acgacgttcg gggagagtca cggcagggct gtgggtgtgg tcatcgacgg
    21061 ctgcccgccg gggatgcccc tgacggaagg ggatatccag ccctacctcg accgccggcg
    21121 gccggggaag agcccgcttg agtccgggcg cagggaaccc gatcgggtgg agatcctctc
    21181 ggggacgttt gaagggcgga cgaccggcgc cccgatcgcc cttatagtcc tcaatcagga
    21241 cgtccgttcg gaggactacg acgcgctcag ggatgtctac cgtcccgggc atgcggacta
    21301 tacctggcag gcgaagtacg ggttgcgcga ccaccgtggc gggggccgga gttccggccg
    21361 ggagacggtt gggcgggtcg ctgccggtgc ggtggcgatc cgctggcttg cgccgcgcgg
    21421 catcgcgatc cgtggtgagg tcgtcgaggt gcacggggtg aggggacgtg aagcgatgga
    21481 ggcagagatc cgcgccgcac gcgattcagg cgattccgtg ggcgggatag tcgaggtgat
    21541 cgcctcaggc tgcccaccgg gtcttggcga cccggtcttt ggaaaaatcg atgccgccat
    21601 cgccggcgcc atgatgggta tcggggcggt gaaggccgtc gagatcggtg aggggttcgg
    21661 cgcagcccgt ctccttggga gtgagatgaa cgacgcgatc accgcggaag ggtttgcaag
    21721 caaccgtgcc ggcggcatcc ttggcgggat caccaacgga gaggatataa tcgtcagact
    21781 tgccgtcaag ccgaccccct cgatacgcag ccctcagcgg acgatcgatc tcgccggggt
    21841 tgaacgcgag ataaggatcg aagggcggca cgacccctgc atcgctccca ggatcctccc
    21901 tgttgcagag tgtatgcttg cgctcgtgat catcgacgcg atgctcgaag aggcgaagta
    21961 ccgcggctgg gattagcgcc tgcacagcgg tggcgttacc cgccgcctca ccccaacagt
    22021 tcagcgatct cgcgcatctc ccggatatgg tactgcccct cggccatcgg tctccccgcg
    22081 tggcagaaga caaactctga accgaagagc ggcaggatcg cgcgggcgaa acggaacctg
    22141 ccgcccatga cgcttgtgca gattggtttc tgcgcatttg cggtaaaccg gaggaggtcg
    22201 aggagttcgt ccaggttttc gggccgaacg actatcttcg gtatatcgcc gtacgacctg
    22261 agcgtcctct cgatcctctc caggtctgcc tggccgggca tatcgtctgt gtggagggag
    22321 gccaggatcc tggccccctg gctcctgagg gagggggcat gcttgctgta gcgtgtctcg
    22381 acatcaacta tatcgacgta gcctgcaatc ggcccgacta tcccggccca gaggtcggca
    22441 tcgccgacaa agccccctcc ttcctccctg ctccttagcg tggcgatcag ggggatggcg
    22501 gtcatctccc ggattgcacg gacgtgattc agcaggtcgc cctccatacg gtcgagcctg
    22561 atctcgatga acgccgggtt gtattccagc accccgctga tgagggcggg gtcgtccact
    22621 gatacgacga tcttcatacc tgctggatgt attccatgct gtatcactta aatacctgtc
    22681 atgcaccctt ttgcgcctga gaatcctgag gctgccagaa cagagtgtct tcaaggccgt
    22741 gccctggttt gatgaacccg gtcatggacg acggatccgc cggggttctg cccatatccc
    22801 cgctatcagg tctgcggctt accggtctca ccgggttttc acgctcacgg cccgcaggca
    22861 ggtatatatt gtaaaaaatt actatgggta atcaaatgga gatcccaact ccagcggaac
    22921 ttcgtgcaaa aaggatccag atgggcttaa aacaggctga tgtggccaga atggccggaa
    22981 tcagccagtc catggtggcg cggatcgagg cgggcagcgt ggatccgaga gtcagcacgc
    23041 tcgcaaagat cgtgaacgtc ctgcaggcag cggagaagtc ggcggtaaca gcggccgacg
    23101 tgatgcactc ccccgttttt tcagtcacgc cggaagatcc cgtcagccgc gccatcgaga
    23161 tcatggggaa aaaaggcata tcacagttgc ccgtgctcga gaacggggtg cccatcggct
    23221 gcatatccga gtcggccatc acgaacgcaa tggaagaggg aggacttcac cagaaatgtc
    23281 cgcgggtggt gagggactac atggaaccgg gtttcccgac ggtcccccca acaacgccga
    23341 tagacacggt cgtccacctg ctgcgccaca gtcatgctgt aatcgtcatc gagaaaggga
    23401 aggtgcaggg tgtgatcacc aggcacgacc tgatttcgct gataacgcag gttttttagg
    23461 atcaaagcag ggctaatccc cgccatttca atggcgggat acagcccgtc cgccagaaac
    23521 tataataacg tatggtgcat gtagtgctat gagattgggg ctgcaatctc aggggtaaca
    23581 tcccaaggat gcctccgacg agaggagggg gagcagtcac accagggaga ccaggtcacg
    23641 gaggaccgcc tcggggtctt cagccttgac cacgcnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnaccag ggagaccagg tcacggagga ccgcctcggg gtcttcagcc
    23821 ttgaccacgc tcgaggcaag gagaacgccg catgtcccga gatcgatggc gatcttcacg
    23881 cactcgccgg actggatccc tgcccccgtc aggactttta cgttcgggtt aaccccccgg
    23941 acagcgttga cggaacgctc gattataccg ggatcggcct ttgagaccga taccccgctc
    24001 ccgatcagtt cggggggttc gatcgccacg tagtcggggg cgagcgctgc ggcggcagcg
    24061 ctggtagcat cgttgtttgt gcagacgacc gactcaaggc ggagcctcct ggcagcctga
    24121 acacaggcgt cgatatccgc aagggtgaga cggcgctcgg agtggttgat cagggtgcca
    24181 cgggcacccg cagaccggac agattccgga agaatatgac cggtatgggc accagggctg
    24241 atcgggtcga tatgctgggc gtagaccggg atcgagtagt ggtggctcat cgggtggagt
    24301 tcggtgaagg tcggcgcgat gccgatgacc gctccgctct cttttgcaac ggtctcggcc
    24361 gcagcagcta tccgatgggc gttgctgccc ataccttcct tgtaggtctt gaggttgatc
    24421 aggatgaacg gcgaatccat atccatcacg tctgcctgat aggttgctcc accctgtatt
    24481 aactctgatg caggccgcgg tctttcatca accccaacca atcctgcggg acgaccctca
    24541 accctcaata cccctgacaa aatgcccgcc ggtgatgccg ccgagagccc gccgtttcac
    24601 ctcgttcctg agcccgggca gtagaccgaa gtccccacgg gccacagcat cgaccgcggc
    24661 ccggtagaag cgggccatct ccccggcgta gcgggggccc cggccccgtg catcgatggc
    24721 aacggagtct atacccatct ccgcaatctc cgggaggtgg tcgatcaggc aggtctcaac
    24781 cgcgtttgcg atgcaagtcc tgccctcaca gtcgcacctg agcgggaata cccggttgcg
    24841 cccatcctgc agcccgataa accctctgct gcaacctgct tcgccggcga cgcctcccac
    24901 cagccggtcc tcgctcacca ttgcctcgat gctgccctgg accagcacct cgagttccag
    24961 cgtcccgccg gtcgaggctg caagcccggc gaggtcgtcg gccgggagtt ccggggatgc
    25021 tgtgcaccgc aggaacagcc catccagtct cgcggcggtg cgatggttcc agaggttcag
    25081 tccggcggcg ccgtagaggt tcatccgcgg ttcggcccgc ctcacagcat cagcagcgcc
    25141 catcccgctc accatcaccc cgctaacgcc tgccccgtat agcgacggca ggatgggcgt
    25201 cgccgcgtcc aggaacgacc gccgtgtgat tgcaggccat ttccagacaa cctccgcacc
    25261 gccgtcccgg cagacgctgg cagcctcttt gaggagagag ggaacggcgg ccacttccag
    25321 gtagaccatc cccgcccctc cactgacagc agcctccaca ccctccaggg cgtcggtgta
    25381 gaccgagacc gacgggaccc tgcgtccccg gttgagcgcc gctgcttgtt ccatcccggt
    25441 gagcgccctt tcaagccgtt gccgggccgc atgaaccgcc tccgcgtccg ggcgtcgcgc
    25501 ggcaaggacg gcctcctcaa cccgctcgag aaactcccgc cggacgcggt tcaactctcc
    25561 gagcggggca aagaggccgc ccgggtagcg gatatccatg ctccttatca caaacggcgt
    25621 ccccccggtc ttcccgaact gtgcggctat ctgctcctcg gtcagcggcc ggttccgtgc
    25681 cgcatccatc tggagatcgg ggcggtaacg cacccgaagc ggtttgccgc ccggcaactc
    25741 gacgaccgcc tccaggcagg gcgtcgctcc atcccaggtg accaggagat ctagttgcag
    25801 cggttcgggg ggcgcctcca ttatcctctt tgccccgtcc tcaagatcca tgctctttgt
    25861 gaggaagacc cgtgcaccgg gcgttaccgg cgcagggacg ctgagtcgca gcgtgccgtt
    25921 cctgacggcg gaggtccgcc ggacaaccgc tcccacatcc atcgcagggt catctctgca
    25981 gaagaccagc ccgtcgcctg aacggggcac gatatccccg gcaaggcgga cggttgcctc
    26041 ccctctccgc gggtcgtaac cggtgacccg cccgaggaga agcccccggt tcccgggccg
    26101 gtcgcgggcc atgatatcgc gggcgccgag gatgtagccc tccgtgaact cccggttgaa
    26161 cgcgagcgcc aggtcccgca gatcctccct tgatggcgac cagtcgttgc cggcggcgat
    26221 cgcatcgaga gcacgccggt agatccgggt cacaatggcg acgtaggcgg gagaacgcat
    26281 cctgccctcg atctttagcg aggccaccgg tgcccgcacc agcctatcga ggtaggggta
    26341 gacgcagagg tcccgcgtag atagaaggta tcggtcattc acggggaggg gctccagatc
    26401 tcccggccga ccgtattcgt ccaccccggc ggccacgagc cggtagggtt tccggcacgg
    26461 ttgggcgcac atcccgcggt tcccgctgcg gccgccgatg accgaggaga ggaggcactg
    26521 accggagtaa gagtagcaga gggcaccgtg ggcgaagacc tcaagaccca cccctcgtgc
    26581 accgggatcg gccgctatat cttcgatttc tggaatggtg agttcccgcg ccagaaccac
    26641 cctggagaag ccttctccag cggccctcag aacgccttca cggttgtgta tcgtcatctg
    26701 ggtggatgca tggagtggga ggtccgggac gacctcccgt gcaagcgccg ccgcaccggg
    26761 atcctgaacc aggacggcgt ctgccccgat ctcgtagaga cggttcaggt atcgggccag
    26821 atcggagagt tctgcatccc tgaccaggat gttcacggtg acatagaccc tgacaccgcg
    26881 caggtgggcg taatcaatgg cggagacgag ttcctcctcc gagaagttcg ccgcgtagtg
    26941 ccgggcaccg aaccgtctcc cggcaaggta gacggcgtcg gcaccagcgc tgactgctgc
    27001 agccagagcc tcaggagacc ccgccggcgc aagcagttct ggcaaccctc caccacgaag
    27061 atgatagtac ggcatctcta tgttagggtt cgccccggat gcctatatac tcgttccagc
    27121 ggcgcaggtg tgtgttccat tttaagagtt tccctgcggc tataccccta aattgccccg
    27181 gataatccaa tttgcacggg agggggacaa cgattccggt gtacaaaccc gccctccccc
    27241 cgtcgtccct ttgagggcgg cgggcaactc actgcccgat ctttaacagc atctcttcaa
    27301 gtgtcgggta gtgcgactcg atccgctcta tcgttgcgcc ggcggccgca agcgtcgcgg
    27361 tcatcctgtt caggtcttcg atgctgtgcg cctccgccat gtagcggcca ttggaggcga
    27421 tgtaactgac cggggttgaa aacgtcgcgg gatcggggat cctgaagaag atctggtagg
    27481 tgatcgagcc aaatgcctcc cggagttcgt gcatgctgcc ttttgccacc accttccccc
    27541 gccgcagtat caggatcagg tcgcaggcct cttccacctg gaagaggttg tgggcggaga
    27601 agaccaccgt cttgccctga ttgcgcagcc ccttgatgta gtcgagcacc gagcgtgagg
    27661 tcatggggtc gaggccggag gtgggttcgt cgtatatgag caggtccgga tcgtggatca
    27721 gcgatcgcgc tattgcgacc ttccgcttca tccctttcga gagttcaccg atcttcttgc
    27781 catcgggatc cagcatgagt tcgtcgagga gttccctcga ccgtttcctg attacgtctg
    27841 ctggaagacc gtagatctca ccgaagaacg caaggtacgc ctcaacgctc atggtctcgt
    27901 agagcctgga ctcttccggc aggtatccaa ggttccgttt cagatcgagc ggtcttttaa
    27961 caacgtcgat cccgtttatg acaagactgc ccgccgtcgg cgagataagt ccggccatga
    28021 tctttaaaag agtggtcttg cccgcgccgt tatgcccgat caccccgaag atctccccat
    28081 cgtcaaggtc aaacgagacg ccgtcgaggg ccgggaactc gccgtaatac ttgacaagat
    28141 tgcgggccgt tatcatccgg tatctctttt gtctccgatg cctactaata ggttgctcta
    28201 cagaaatatg cctctcagac accctccggg atcaccatga gtcttatctc ctcaatccgg
    28261 acgatcgcca cctgggagat aaagcgatcc atgaccacga tggggagagg catcctgccg
    28321 attgctgtgg ggcttcttct cctcctggtg ctggtgacgg catttgccgc agagagcggt
    28381 ctccatctac aggacgggat gtaccggatc ggcgttgatg accccggcac ggccagtata
    28441 gtcgcatccg acacccggtt tgccacatac ctggatgatg cagcgtcgct ctgggagaac
    28501 aggttttcct acgatatcgt catcataaac ggggaggtct acgctgctga cacggataaa
    28561 gggcgtgcgg cattaaatac gctcgagcgc gattacgaga gttacgttgc aagcgtggcg
    28621 gcaacggaac ccgacctctt cgccgcctat cctctctgga tcgatctcca gtacgtgaag
    28681 agcgagatcg atttccttgc gacccagagc ggccagcagg tcggcgcccc cgtggagacg
    28741 aggagacccc catctccatc tctcccggtc gaggaggtga cacctccatc ccagggcagg
    28801 ctcaccaccg aggaagaact cagggagcat ctcgtggagg ccggggatgg tcagcccctc
    28861 aagcgctaca catctctcat ctctggcgag tccgccacag acagactcct gacaccctca
    28921 gaactctctt tgccgctgcc gtttgatacc atcatcctgg tcttcgtctt catatttccg
    28981 ctctacttca cctcccagtt cttcatgatg agtgtgatga acgagagggt gggacgcgca
    29041 ggtgaggctc tcctctccac ccccctccgc cccgtggcga tcgtcgtagg aaaggccctt
    29101 ccctacttca cgatcatgat cctgatcgcg gccgcgatca ccctctttgc cagggcgccg
    29161 cttgcgatcc tccttccgct cgtcccggtc atcctcttct tcctggcaaa cgccctcatc
    29221 atagggatga cgtcccggag tttcaaagaa ctctcctttg tctcgatctt cttctcgacc
    29281 gtcgccacgt catacctctt cttcccgacg gtatttgcaa acacccacgt catcagcatc
    29341 gtctcccctc tcaccctggt cgtagttgca atccagggtg acgggttcac cgcgatggag
    29401 tacatctact cgaccgcgct cttctttgca acgagcatca tcctcttcta tgtcgggatt
    29461 gcaaacttcc gggaagaaca cctcttctcg gagagacgcc tgacatcacg ggttgttgac
    29521 ttcatatcga ccgggatcag ccggaaccac ccgcacttct cgctcttcct gcttgccgca
    29581 ttcatgatac cgttcgtctt catggccgag atgctgacgc tcgtcctctt cttcaacatc
    29641 cccatgccgc tctctctcct cctgctgacg atcactgccg cggtcatcga agagtttgca
    29701 aaatcgatcg ggctttacgc tatagtcagg gatcggccag gttttctcac gccgggaaac
    29761 cttctcgccg gggcaacggt cacaggcctc gggttccttg tcggcgaaaa actccttctc
    29821 tttgccacgc ttgcccagat caccgagtcg atcttcggga gcatcctctt cctctctctc
    29881 caggcgctct ggatgccgct cctcctccat atcgccgggg tgctgataac cggtgggttc
    29941 ctgctgattg gggggcggcg cgcctatgcg ccgggtgtga ttgtggcggg gatcgtccac
    30001 agtctctaca acctctactt catcgggggg atgctccttt gaagaggcgc catatctctc
    30061 tgatagcaaa gaaagagatc gccggactgt catcggagaa gactatcgtc tttgcgatcc
    30121 tcctccaggt cttcatagcg atgttctcct cgttcttgat ggtgggactc accgcgatgt
    30181 atgacccgga ggcaatcgag ggctactcga cggtcaccta cagagtcggt tacgccgggg
    30241 cggaatcacc gctgatcgat gagtttggag aacgagacga tctcatcctt tacaggatgg
    30301 atctcggtga ggcgcttgcg gcactgcgcg agcggatgct ctcggcggtg gtctacgtcc
    30361 cggagacgcc tcccgacgct gaaggcccga tcacagtaac gctctatctc atccagaacg
    30421 accttcagtc tggtgtcgtc aacgtcaaga taaaagaggt tctccagggt tacgaggaac
    30481 aactccgtga gacccgggcc gaccggctga tctactcacc tgtggagttg aacttcccgg
    30541 acaccgatgg cggcgagagt ttctttgagt ttgtctacgg tctcctcatc ccgctgcttg
    30601 tcttcctccc ggcgatcgta tcggcggccc tgatcatcga cctgatcacg gaggagtatc
    30661 agcggcagac gctcgagaca ctgctctcga caccgatcac gttcacggag gtgatctggg
    30721 gaaagattgc cgcctgcgag gttctggttc ctctccaggc gggcgcctgg ctcatcctcc
    30781 tctggctaaa cggcatcccc gtgggtaacg ccatggcgat cctcctccac gtctcgacgt
    30841 ccggtctcat cctcatactg ctcggggcgc tcgtcgcgct ccattaccgt gagaggacga
    30901 gtgcccagtt catattctcg acggcgctgg tggtggtctt catcttcgtc atggcagtcc
    30961 cccacaatcc gttaaacctc attgtaaggc tggcggcgga ctccatcggc cccgtccact
    31021 ggctggttct ggcgctggtc gcggctgcgg cggcgcttct ggggtgggcg atcactctct
    31081 acgcccgccg ggtgggggag aagacaccct gagggagcgg gttataggac ttggtttcac
    31141 cgcagtgaga cgggacacca ccaccttttg tatactgtca gtgcgataac tgtatcatgg
    31201 agatcacgat cctctcacca ggtatctaca cctacggcgc catgctgata ggcggcgttc
    31261 ttcgtgacgc aggtcaccgc gtgaccctca cccggacgcc tgaggttcct atgggttcaa
    31321 tcctgcttat gagcctcttc tcgacccagc acctcctcga tcccgcgatc agggacgtga
    31381 tccgggcaca ccgcgagcgt ggcgggcggg tctacgtggg ggggccagtc tcggccgcac
    31441 cggagatggt gctcggtgag attgcccccg acgcggttgt cgtaggtgag ggtgaggaga
    31501 cagttgtccg tctggtcgag gagggggtct cgccagacct cccgggaata gcgtttctcg
    31561 atgacggcag aactgtggtg acgcctcccg cccctccagc accgatcgag cgcccgctgc
    31621 ccctgatccc ggacgatata ggcagccaga gtatccgggg ggcgagcgcc tacatcgaga
    31681 cccaccgggg gtgtataggg gggtgcacct tctgccaggt gccccgattc ttcgggagga
    31741 gggtcaggag ccgatcgatc gagagcatca tcgaggaggt gaaggctttt gcagcccacg
    31801 gcgcaaaacg tctttctata tccgggggga cggggtcgct ctacggttcg agcagcgggg
    31861 agatgaaccc ggacgccttc atcgccctcc tgcgcggaat ggcggaggtg atggggccca
    31921 aaaatatctc cgccccggat atcagggtgg actgtatcac cgacgagata cttgacgcca
    31981 taaagcgcta caccatcggc tgggtcttct tcgggatcga gtccgggagc gaccggctgc
    32041 tccggatgat gggcaaaggc gtgaaggtga ggcaggttgt ggaggcggtg gagcggtgcc
    32101 ggaaacaccg gctccatgtc gccgggagtt tcattgtggg gtatccggga gagactgagc
    32161 gggattacat ggctacaaag gatctgatcg ccaccctcct gcttgacgac acctttgtga
    32221 gcgctgccga acccataccc ggaacgccgc ttgccgatct cgtggtaaag acgccacgcg
    32281 ataagaatcc gacttttatg ccgcataaga gtgattaccg ggcgctccgt ctgaccgaga
    32341 gcgaggcccg gtgtttagac ctgatgatgc acgctgacct cttccgcaag cggatccgga
    32401 ttgttaacga cgagatctac gacgcttacc ttgccgaggt aaggaagcag ggaaaggaga
    32461 tccgggcggc aaccgacctc atcctccggt atgccggggt ttaaagggtt tccagccaga
    32521 tcactcccct tcgggcggtc ttcgtaccgg gaggtgccgg ccgccttaca ggatcaactt
    32581 cgtctggatg gtctccttcc ggttgccgaa ggtgaggtgg cgtgcggcga cctgccccct
    32641 gattccagca cgtgcaaggt cggccgggcc ggtgaacggc cgctgtttgc gaagtgcgat
    32701 gatcctgtct gctgcctttg gtcctatccc cgggacgcga agtaaatccc actttgcggc
    32761 ggtgtttacg ttcaccggca tcatattggc tgcgatgacc tcttttgggt cgcggttttc
    32821 gaagaacccg tcttcgttga gagaggcctt gagttcgtcg gccgtaaaac cgtaatccct
    32881 gagcaggtag tcggcctggt agcaccgtcg cgcccgccag gccggcgtat ctggatgctc
    32941 tgcgagagga gttccgggaa gggagcggaa cgccgagaag tagacccggg ctggcctgac
    33001 ccggcgatac tggccggcaa gacaggagag gatctccgca tcggtctcgt ccgccgcccc
    33061 gatgacgagc tgcgttgtat ggccctcggg cttctcctcc gccacccagg cctgccgttt
    33121 caggatatcc tgctggtaat ccttcacccc ggcgatgcac cccaggcggt ctgaggacgt
    33181 ggtctccagg ttgatgctga tcctgtccgc caccctggcg gcgtcatgga tatccgcccg
    33241 tgcagcgccg gggaggatct tgaggtggag gtaaccgtcg tagcccctgc accgcaggat
    33301 ctcccccgtc tcaacaaggt cgtgcatcac aaggtcgacg tcgtgcggga tgccggaact
    33361 caggaagagg cctccgacaa ggtcttcatg ccagaggcga aggaaggctt cggcaaactc
    33421 atccggtgag aaccccagcc gttcacgttt taaccggacg gaacagtagg cgcagtcgta
    33481 ggagcaataa ccgttgagaa gcaccttgag catccggccg cacccgcccg cccgccggtt
    33541 gtaggttatg tgcgctgctg tagagaggac atggatgcac tcgccgggtg cgcagatgtc
    33601 aaactgcccg gcggcagtaa ggctggagag tttctgctcc acaatggaca tgactggatc
    33661 atctgtccgc aggtaaaaga gcctgtcgcg cgcggggtga aagtgtttat tggtcgctgc
    33721 cgccagggag cgaaatatcc acgttcatta tatattaaga taaatatata ttttatggaa
    33781 gagcatgtca ggttctcgac aggatcagaa gaatcaaata cgttcttccg aagcaggagc
    33841 cgcctttacg cggcataccg tagagagttc caggcggcag tcagttcagg ggtattctcc
    33901 gtgcactgac gcctgtgatg ataccgttac agcgctcggg cgcagcctgg tccaccacgg
    33961 tccgcacaac gaccgtgcct ttctgtatca tctggaccca acagacctcc ccgagatcac
    34021 tggaaggctt ggggcgtttg caaaagagcg cggatattcc cgtatatttg cgaggatacc
    34081 ggccaccgcc ctcgatcagt tcgtatcagg cggctacacg ccgctggtgc gtatcccggg
    34141 attgtttcgc ggcgaggtgg acggctgcta cctggcgtac cgccatccca cgcggggcag
    34201 caggaggagc gggaacgatg aagtccttgc ggttgccatg gccaaagccg gagagcggga
    34261 tgcggcgtgt ccggcgctgg aaccgtgttt caccgcggtt cgtgccactg cagtggacgc
    34321 cccggcggta gcgtcgatct atcggagtgt ctttgagacc tatcctgtcc cgattgatga
    34381 ccccggttac cttgcgcaga agatgcagga gaatctgcgc tgtttctgta taattgagcc
    34441 agggggtcgt atcgttgcgg tcgcggcagc tgaagttgac gcccgcgccg aatttgcaga
    34501 gatgacgggt tttgcaactc tgccaggcta ccgggggcac gggtttgcag ccatcctcct
    34561 ccagttaatg gaggacgatg tctcctcaag cgggataaag acggtgtttg ccatcgccag
    34621 ggccaggtca tacccggcca acatcacgtt tgccagggca ggatacgcat tcgcaggggc
    34681 gctctctgag agtgtcaaca tctgcgggtc ggttgaggat atgaacgtct ggtggaagtc
    34741 gataggcgtt ttggaccgat ccaggcagac ggtccggcgg ggaaacaata tccggtttca
    34801 aggcaaacct tta
//
LOCUS       DAOSZX010000005        32705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1118_length_32705_cov_21.721440, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000005 DAOSZX010000000
VERSION     DAOSZX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 32705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32705
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1118_length_32705_cov_21.721440"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK196_00980"
     CDS             <1..299
                     /locus_tag="PK196_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON40031.1"
                     /translation="TELVKVDPRDTTQMCSNCGSIVKKTLSERVHECPYCGFVADRDY
                     NAAVNIHRVGMEQPFEPVEPRPLHHISVVQVLAMKQEAPPERRGVVHMRWLALF"
     gene            281..712
                     /locus_tag="PK196_00985"
     CDS             281..712
                     /locus_tag="PK196_00985"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HON40032.1"
                     /translation="MVGAVLDARRSDDLVPLKVTADLYIEADDGRRFYFEIKSPMPNK
                     GQCLEVTQRLLRFHLIAGAARPDVHGYFAMAYNPYGPKRDDYNWNFALNYTPFAEAVL
                     IAQEFWSIVGGEGTMEELLSIYQEVGREKTKYMLDSLAFGF"
     gene            1317..2747
                     /locus_tag="PK196_00990"
     CDS             1317..2747
                     /locus_tag="PK196_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40033.1"
                     /translation="MSDYHHNEFLGFGTCAPPNVLPNWFYKWLFFPPIKTEGGVPVAA
                     PYGLRKIEAELALEGFDAVVVDPDHLGKYLENAKVLSIYVMDPFGLGPASSTFAFFLQ
                     KEPFLAEYFRKLMSSKEIKEAKERGLKVILGGPGVWQFKYRPEFAMKYGIDCIIDGEG
                     EKVIGKIVRAALNDENLPEYYEVPPEEVPSLEEIPGIIAPSINELVEIGRGCCRGCRF
                     CNVTLRPLRWYPYEKILKEIEVNRKTGNGVCLHAEDVMLYGSRNTTPDTEKLTRLHEI
                     VSDKCDGIGWSHCSLAAVASSPKLFSQVAEIILQKQSWWGAEVGIETGSAELAKKIMP
                     AKAHPFKVEEWPEVIRTGMGLMHDNMLVPACTLIVGGPEETEDDLIRTIEMMDVLRDI
                     RSLIVPLFFVPMGRFRNEEWFDETEMNGLHQELLLRCLRHDLYWADSLIDLSIGRDWP
                     GKLRSSFYKLFVGLVKRRIEREGVEI"
     gene            complement(2930..4771)
                     /locus_tag="PK196_00995"
     CDS             complement(2930..4771)
                     /locus_tag="PK196_00995"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HON40034.1"
                     /translation="MRLLLIHSDHIEYEARKKTKAAEEDAVLKDGLDEALAVFCAVES
                     LDEENIDDAVRQAVEEIVNTSRQLGTTNIMIYPYAHLSSDLASPEVAVSALRDIEEAL
                     REQEDDFVVRRAPFGWYKAFSLSCKGHPLSELSRTIVPGGGVAAAAKKEIEHEFFVIT
                     PEGERRDAADYAAGNTPFACLVQKEIGYAGQEGVEPAHVDLMRAKELVDYEPRSDVGH
                     LRWMPRGKLIRDLLADYVLSKVLDYGAMPVETPVMYDLADKAIAEHAARFGERQYRFK
                     SGNRDMMLRFAACFGMFSIMNSMHISPNTLPMKLYELSTYSFRHEQKGEVIGLKRLRA
                     FTMPDMHTLCRDMDDALAAFEEQLAMGWKSGRDLDTPLVGVFRCTRDFFEQYEPWVKK
                     IVANSGVPMLIDILSERSHYWIAKVDLAAIDAQGRPIENPTVQIDVESSERFDISYYT
                     PEGQEIHPPILHCSPTGSIERVICAMLENTATQEVPSFPTWLAPTQVRLVPVAERHVC
                     FAEDIAGRLKAAGIRADVDDRDESVNRKVREAGVDWVPYVVMIGDLEAETGRLTVTIR
                     RLSEKKQPYRETMTESELIQAVRMETEGKPFRPLYTPGCLSRKPRFI"
     gene            complement(4908..5465)
                     /gene="dcd"
                     /locus_tag="PK196_01000"
     CDS             complement(4908..5465)
                     /gene="dcd"
                     /locus_tag="PK196_01000"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HON40035.1"
                     /translation="MILVDWQIEDRIRRGHIKVEPYDPDLIQPNSLDIRLGSHFVWYV
                     PGDMVIDPFEKETIFAGTEETVADSIVLAPGQFLLGETLESIDLPDDIVASIEGKSSI
                     ARLGVELHQTGGWIDAGFRGTITLEMCNVNSRPVRVYAGMAIGQLVFYHTDRAARPYY
                     MKKDAKYMNQRQATLSRYHENARRT"
     gene            complement(5488..6186)
                     /locus_tag="PK196_01005"
     CDS             complement(5488..6186)
                     /locus_tag="PK196_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HON40036.1"
                     /translation="MPEISIVLVEPLYEGNVGFTARVMKNFGFDRLVLVNPCALGDEA
                     FMRASHARDVLDSARRMTLDDVYREFDFVVATTGEVSKSVCTPMRMPYYTPAEVREVI
                     ADIDGSVAILFGRENWGLSNAELERANLICTIPTSEVYPILNLSHAVGILCYELAGLP
                     RGTYLLAGPVEMESLYRHIDAFLDRIEHPDFKRENTLTLIRRVLGRTKLTTREVSTLH
                     GLMRRAEWHLENKE"
     gene            complement(6196..6891)
                     /locus_tag="PK196_01010"
     CDS             complement(6196..6891)
                     /locus_tag="PK196_01010"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HON40037.1"
                     /translation="MHANWKTWTHVTKLDPDKRLPGGAIEEIATSGTDALMLSGTQNV
                     TRDNLLELLDQVSPYGLPVVVEPASPDCAIFDGGIDHLFVPSVLNTNDVRWIVGKHYT
                     WLLNNGDGINWDMVVPEAYIVLNPDSAVGRVTGADCNLSAMEVAAFAQVADRYFRFPI
                     VYIEYSGRYGDPQIVRAAAGAIKDALLYYGGGIRSAEQAAEMGRYADTIVVGNAVYEE
                     GIEVLRATVRAVH"
     gene            complement(6898..9693)
                     /locus_tag="PK196_01015"
     CDS             complement(6898..9693)
                     /locus_tag="PK196_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HON40038.1"
                     /translation="MHLIIAEKNISAKRIAQILAGNEKVKTTKDGSVFTYSFDNRTVV
                     GLKGHVVEVDFEPGYANWRSTTHTPRSLIDAGMVKKPTEKKIVNLIQKLAKKADLVTI
                     ATDYDTEGELIGKEAYELVRAVNPDVRINRARFSAITPAEIRAAFENPVELDFNLAAA
                     GEARQTVDLMWGAALTRFLSLAARRGGRDILSVGRVQSPTLAMIVDREREIENFVPQT
                     YWVLSLTTEKDGTAFEARHTTGRFTEREAAFTAKERTKEPLVVTDVEEGQKVDRAPTP
                     FDTTTFIVAAARLGFSAANAMRIAEDLYMNGYISYPRTDNTVYPGSLNLNGILEALKG
                     GVFDKDVAWVQQNRRPTPTRGKKESTDHPPIHPTAAASREALGEERWKVYELVVRRFL
                     ATLSPDAKWATTRYTFDASGETYRATGGRLIDEGWRRVYPYSEAKETILPEVKPGERL
                     PIIDVKLEEKETQPPARYSQSRLIQVMEELGLGTKSTRHEVIGKLISRRYVEGNPLRP
                     TLVGRAVIDALDNHAATITEPEMTRTLEEHMQLIKQNRRSRDNVITESRQMLHQVFDN
                     LEAHESEIGNEIMEQTAEEHTVGPCPVCGRDLRIRHSGVSQFIGCTGYPECRFNISLP
                     GTAWGRAIRTDETCEKHNLAHIRLIKKGARPWDIGCPLCSHISSNIEALFLMPSMTDD
                     LLQRLHEQHIYTVSDIAGMPPEELAEVLGVDIEEARGLAGEAEDALEILRRRSELRKF
                     VRKIVPPKKGRSYVRIFKNLLEEGIGDIHALSQAEVATLKRAGIPETAANELLDEARR
                     LSSERALREAGVPSVSLKKYLEAGISSPEDLCYLPIPYLAGKTGINPETVHKHVDLVC
                     RYLGRPTPPKITRQVIERGRKELLGVPGLGEATLQRLYLAGVYDAKTLLEADPEMVSS
                     ITGIPKARIHDYIDHLK"
     gene            complement(9699..11114)
                     /gene="gatB"
                     /locus_tag="PK196_01020"
     CDS             complement(9699..11114)
                     /gene="gatB"
                     /locus_tag="PK196_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HON40039.1"
                     /translation="MKTIIGLEVHVQLSTATKLFCGCSTDYRDDEPNTHCCPICLGLP
                     GALPRLNIRAVEHGLKVAKALNMEVLEESEFARKNYFYPDLPKGYQITQYDKPLAVAG
                     TVEIEDDEGHEKIVRISRIHLEEDPGRLIHVAGGSGYSLVDYNRSGIPLLEIVTEPDL
                     RSPQEARRFLNRLRAILEYLEVFDGEREGALRVDANISIEGSERVEVKNISSYKGVEK
                     ALTFEVTRQRNLLRRGQKVGRETRHFMEARGITTSARSKEGEQDYRYFTEPDLRPFRV
                     ADWVDGIDLPELPTARKERFMAEYGISLNHARTLTGDPRLADFYEEVAYVDPFLAATW
                     VADTLLGELNYRGMRITDVPANRFIELLALLRSGTITDRVGVQVLREMLDDCAAGRPC
                     EPPAVIVKREGLVRGEGDEFAVVIGDVVAANPQAVADYRAGKKGALNFLVGQVMKETR
                     GRADPRELGRMVAEYIDKGEV"
     gene            complement(11111..12415)
                     /gene="gatA"
                     /locus_tag="PK196_01025"
     CDS             complement(11111..12415)
                     /gene="gatA"
                     /locus_tag="PK196_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HON40040.1"
                     /translation="MAGTLEFSPDDRYNAFITTCSEAPFSDGVLAGTAIAVKDNISTA
                     GIETTCGSRILEGYIPPYDAHVVELLKRAGAAIVGKTNMDEFGMGTTTETSAFGPTTN
                     PIDTSRVPGGSSGGSAAAVAAGLVPMALGTDTGGSIRCPAAFCGIVGFKPTYSRVSRY
                     GLIAYANSLEQIGPMARTVEDVSRLLSVIARYDPRDSTMIDRPYDHQPSANVDGLRVG
                     IPEEYFGEGVDARVAEAVRDAIGVLEDLGARTVPVSIPGMRYALAAYYVICTCEASSN
                     LARFDGIRYGPAADIRKPWHEAYREIRREKFGTEVRRRIMLGTFALSAGYAGRYYAKA
                     QIARRNIRLDFERAFRSADIIAGPTMPTVAFRLGEKTDPLSLYLSDILTVPANLAGIP
                     AISVPCAGRVDGLPVGLQMMGRRFEDERVIDAAYAYEQGAGR"
     gene            complement(12416..12685)
                     /gene="gatC"
                     /locus_tag="PK196_01030"
     CDS             complement(12416..12685)
                     /gene="gatC"
                     /locus_tag="PK196_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HON40041.1"
                     /translation="MITERDIEHIAELADIGISKEEISEFTSQFNAILEYFEVLDTLE
                     GESAPESGTVNVFREDEPRPSLPQEKVLENAGSTENGFIKAPRVM"
     gene            complement(12675..13673)
                     /locus_tag="PK196_01035"
     CDS             complement(12675..13673)
                     /locus_tag="PK196_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HON40042.1"
                     /translation="MRLKGWIEHGGVRLTPAELERMLAANPESLARCGGEFLIEWDDC
                     TARDNLGIMPGPAPPGTICCGGRPVGRINPDPPSCSLEEAIVTAIGLRSDEGVVAFSG
                     GVDSALIAHLARLPCVTVGLPGSHDLAHATKAARMMDLDLTVISPGKDEVADALFRVV
                     RVIPGQINPVDASIATTLYFVAALAKEQGHTRILVGQGADEIFGGYARYLSSTDLAAE
                     LERDFADLARQRARDQAVAALHGAYFSMPYQDLRVVRAAQTIPASMRVRGGVRKYPLR
                     VIAERHIPAENAWTEKKAMQYGSGIYRTIQNLARDNGYKKSIQRYLTEISRAEHDH"
     gene            complement(13992..15089)
                     /gene="fbp"
                     /locus_tag="PK196_01040"
     CDS             complement(13992..15089)
                     /gene="fbp"
                     /locus_tag="PK196_01040"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HON40043.1"
                     /translation="MVKTTVSVIKADVGSFPGHSRTHPKILETAARRLKEAEGNLIID
                     SYVTHCGDDLELIMTHNRGEDDEEIHRLAWDIFMECAGIAREMKLYGAGQDLLSDSFS
                     GNVKGMGPGVAEMEFEERGSDPVLIFMADKTEPGAWNYFLYKIFADPFNTSGLVIDPS
                     MHDGFIFEVHDVMKKRRIQFRTPEESYSLLAYIGAPSRYVIKYVWKKNGLIAASTSTQ
                     RLSLIAGRYVGKDDPVMIIRAQSGLPAVGEVLDPFRTPILVAGWMRGSHHGPFMPVGV
                     CDANCTQFDGPPRVICLGFQVCNGRLIGPADMFDDPAFDGVRLQCCELSNMIRAHGPF
                     EPHRLSLSEMEYTTLPGVEKEFKDRWEDIPQ"
     gene            complement(15248..15493)
                     /locus_tag="PK196_01045"
     CDS             complement(15248..15493)
                     /locus_tag="PK196_01045"
                     /inference="COORDINATES: protein motif:HMM:NF037143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HON40044.1"
                     /translation="MEPTVEIYTTDNDVTVAADLPGFEEHEIRLLFINGTLTIIAGEN
                     QAASIDLPPVDPDSMEARYRHGVLEVRFSRGRPIRID"
     gene            complement(15496..15621)
                     /locus_tag="PK196_01050"
     CDS             complement(15496..15621)
                     /locus_tag="PK196_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40045.1"
                     /translation="MEHEEPGRTIACGFRIGVQDVGKSQAGEGLILRMKRLKPSG"
     gene            complement(15744..18176)
                     /locus_tag="PK196_01055"
     CDS             complement(15744..18176)
                     /locus_tag="PK196_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HON40046.1"
                     /translation="MTNNNSIEVTVKEAAHEDVGRGIARLSLDAMKALNLVSGDVIEI
                     EGRQKAATLVWPGFPQDTGKAVLRIDGSTRGNVGAGIDDKVRIRKIEAGYAKKVVIQP
                     TQPIRLVGGEQYLGRLLRGRPVTEGQLIRINILGNPLTFAVAKVLPKGVAIVTDSTEI
                     ELKETPYEPAKAPKRGREIAGDIHYEDIGGLDRELQLVREMIELPLRHPELFERLGIE
                     PPKGVLLYGPPGTGKTLIAKAVANEVDAHFITLSGPEIMSKYYGESEERLREVFEEAQ
                     ENAPSIIFIDEIDAIAPKREEVKGEVERRIVAQLLALMDGLKSRGQVVVIAATNLPDL
                     IDPALRRGGRFDREIEIGIPDTKGRLQIFQIHTRGMPLADDVDLEEYARTTHGFVGAD
                     IALLAKEAAMHALRRVIPQIKIEEEIPAEIIEQLRVTNEDFIEAHKHVEPSAMREVLV
                     EVPDVKWEDVGGLEDVKQELSEAIEWPLKYPEVFSSLDTKPPRGILLYGPPGTGKTLL
                     AKAVANESECNFISVKGPELLSKWVGESERGVRQIFRKARQAAPSIIFFDEIDALVPK
                     RGTYMGSSHVTESVVSQILTELDGLVELKNVMVLGATNRPDMLDEALLRPGRLDRVIY
                     VPPPDRESRRKIFEVYLRNKDILAKDVNIDELVDRTEGYVGADIEALVREAKLAAMRE
                     FITAMGEKSEEERRQAIGNVRITKKHFEEALGRVKGTLDLERLEDYERQAWKFLYNQE
                     QRSLLEEASSLIKRARLQETGKTEQEVKDLTSALKNAVYARKKDFGEIKRLTEDLKSR
                     LERPMAQMGTAF"
     gene            complement(18241..18816)
                     /locus_tag="PK196_01060"
     CDS             complement(18241..18816)
                     /locus_tag="PK196_01060"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HON40047.1"
                     /translation="MRLTKAELRMRAKEARSRLTPAEIARYSASIERRLIEVLDGFTT
                     VMVYVSKPPEVETRGLIAALNNRGVLVVVPIIERETRSLRLSALPDPGVLVQSTFNVP
                     EPIGHEIPVRPEDIQAVVVPMLGFDSKGNRLGYGAGYYDRFLRRYPHPEKIGIAFSCQ
                     QVECIPADENDIMMDCIITEKVIIEVSDMGT"
     gene            complement(19426..19842)
                     /locus_tag="PK196_01065"
     CDS             complement(19426..19842)
                     /locus_tag="PK196_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HON40048.1"
                     /translation="MKPETEKLVEDFLAQTDDLSDEITEDVREMLGVVPFIFGALRKR
                     PEVFVLSALADYRISRPDSLDQKTAELVAVAAAAGAGAESCLKVHMGAALKEGASPEQ
                     ILDALLIAGMIGKTRILATTLRQFRDVCGEGGGGRE"
     gene            complement(19902..21176)
                     /gene="thiC"
                     /locus_tag="PK196_01070"
     CDS             complement(19902..21176)
                     /gene="thiC"
                     /locus_tag="PK196_01070"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HON40049.1"
                     /translation="MGLIEDARRGVITEEMRTVARAEGVTEDFVRRGVAEGQIVIPVS
                     PYRKVKICGIGEGLRTKVNASIGTSSDIVDIDMEMEKARQAERAGADTLMELSTGGDL
                     TEIRRRVIEATSLSVGSVPLYQAFIEAARKYGAVVNMEEDDLFRITAEQAKAGTNFMA
                     IHTGINYETMKRLQNQGRHGGLVSRGGAFMTAWMLHNEKENPLYAEFDYLLEILKEHE
                     VTLSFGNGMRAGAVHDATDRAQIQELLINAELADKAHAAGVQSIIEGPGHIPIDEIEA
                     NVIIEKRVTNRKPFYMLGPLVTDVAPGYDDRVAAIGAALSSSYGADFICYVTPAEHLA
                     LPTPEEVYEGVISARIAAHVGDMVKLKKRDADLEMGHARRDLDWERQFAVAINPERAR
                     AIRQERMPADADACTMCGDYCALKIVGRHFNF"
     gene            21353..22051
                     /locus_tag="PK196_01075"
     CDS             21353..22051
                     /locus_tag="PK196_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HON40050.1"
                     /translation="MHVNFGGFVPLSTVDWHGRAACTVFLRGCPARCFYCHNISIQDG
                     EDLRDADEIVEMIQGSRMAAGAVVFSGGEPTMQGPALAYLASAARRMGFSVGLHTNGV
                     FPRVIEKLIEERLVDMIALDIKTTWDRYDDLLKLPAANAVRQSLAACKRAKATGSLET
                     CEIVVTLFRGHEAELPEIAKAAGDLDLVLQQGVTAGFVPFSHQELKTLAAPLGRRLRI
                     RTREEGEVVYDPSR"
     gene            22191..22742
                     /locus_tag="PK196_01080"
     CDS             22191..22742
                     /locus_tag="PK196_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HON40051.1"
                     /translation="MKVIGIVGMPGSGKGEVSGIARELGIPVVVMGDVIRERAREAGL
                     PPTDENLGAISAQLRTDLGMDAIARLTIPVIESQGAPVVLVDGIRGDREVAVFREHFP
                     DFTLIAIDSSFETRYRRLAKRGRPDDTLTPDELRARDERELGWGLGRALELADYRIEN
                     EGSLEEFTAEVRGLLSRLAGVDG"
     gene            22739..23512
                     /locus_tag="PK196_01085"
     CDS             22739..23512
                     /locus_tag="PK196_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON40052.1"
                     /translation="MSLIPYFSASSKVWESFDWIYGVEEIGYAGWEIVADGNYRLDNP
                     ANFTAIKECLASTGLLVSVHAPYSDLNLASLNHPIWRESIRQICCCIRYAAELTDRVT
                     IHPGYLSPLGKLAPDMVWRQQKAALDEIGKEAEDCGVLACVENMISIRDFLCRYPEEI
                     LGLTEGIPGIGITFDLGHANTNGLVDAFLAHIGEADHLHIHDNHGQSDEHLALGDGTI
                     PWQKAGRTVARDYTGPVVIEGRNLDEARRSLAAFRRWFV"
     gene            23500..24453
                     /gene="rnz"
                     /locus_tag="PK196_01090"
     CDS             23500..24453
                     /gene="rnz"
                     /locus_tag="PK196_01090"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HON40053.1"
                     /translation="MVCIAGETLHVHFLGTSGALPSPQRNPSCILIRRGSDTLLFDCG
                     EGAQQQMMRARTGFTVDAIFITHWHADHFLGVFGLVETLAFTGRTEPLPVYGPPGVGE
                     FVDVVQRIGRHTRSFPVTAHTLEHGSIVPFNGYTVRAFATLHGIPGLGYLLEEDERPG
                     RFNRDRAIELGVPPGPLFGRLQRGETVRIVRGGVETEVSPAEVLGEPRPGRKVVYTGD
                     TRPLHESLDTAAMLRDADLLIHDATFDDQERERAREVLHSTAGEAGEAADALGAHMLA
                     LVHISSRYTTTTNHIRDARRQYKGNVILPADLTVLEIPYRS"
     gene            24463..25671
                     /locus_tag="PK196_01095"
     CDS             24463..25671
                     /locus_tag="PK196_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HON40054.1"
                     /translation="MVILMEQGLFARSRLILTGLAALLVSAAVSSVAGIYLGSVRENL
                     LLIPGLMVLLPSIIHIRGSIAGVLASRLSSAMHLGEFSVDFGEGSVLGDNIRVSFVVT
                     ILIAFVLGIFAYVASRIFGYPVIGVTDLVLISVVTGILAGTVVTGITLLIALASYRYG
                     LDLDMIAAPTVTTFGDIITLPILMISAAFVMLLDPLARLILGGVAVGLTVAAIIYTLH
                     RPEGIGPIVRENIGLLIPLSVIGTLAGLTYSSSLDILVTTAAFLILIPPFTGILGSIG
                     GILGSRLSTGMHTGEINPTFIPEGNVAHHFIISYLYTLILLPLLALIAHGAAVLMGSN
                     SPGPVMLVIISLAAGLIVMTLVIGVAYITASLSFRYGLDPDNFGIPVITSLIDLIGAA
                     ALVAMIDLLL"
     gene            25701..26903
                     /locus_tag="PK196_01100"
     CDS             25701..26903
                     /locus_tag="PK196_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HON40055.1"
                     /translation="MTDVEYQPVSFKEVLIEMKDISELMVDLAYSAILFESKEIALEV
                     INLEERMNGLVYQARIQSVLGARRLEEAEAMSGMLQVVEAAERIANSASDMAKLILKD
                     IKFPAELKRAMPAAEEIVTRLIVSQGSDLAGRTLGDLKVQSTTGMQVIAIRRGKGWIY
                     DPDKNTRVEAGDILIARGPEAGKEILARMAGMVECPGKEPSSIGEVDDLDRAVQLIIE
                     MKNLSELSVGLAYTSLLFNNKEVAHEVVSLDSRLDDMRYDLDLWILEAARKIDDVRHL
                     RGLLYMSAFTQAISDAAHSIVDVLLRDIEIPPLFKRIVRESDEIITRMRVEPSSPLVG
                     RTLKEASLGTVTGMVVLAIKRGDHWVYRPGKSVRLESGDIIIAKGRRDGECRLYELTG
                     HTDDVSGD"
     gene            26919..28754
                     /locus_tag="PK196_01105"
     CDS             26919..28754
                     /locus_tag="PK196_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HON40056.1"
                     /translation="MQNTDDTIVYRLGATCGLDEVEEGKIYLGWVQGFAPFGVFVQLN
                     DRVKGLVHKTNVKMKHTERDPIIVHVLQVRSNGNIDLEEVTPIVYQTENVIKKMPSVL
                     LGDIGKRVGKTVMIKGEVAQIKQTSGPTIFTIVDESGTANAAAFIEAGVRAYPEIELG
                     DIVAVTGEVMQRNNQLQIEVESMIVLMPEEAAVVRERIEAALDKRAEPREIQFLVESE
                     VLEALRPQMRKVAKEIRKAVFSSRPIVLRHHADADGICAAVAIEQAVTALLRESGADF
                     DAEYFLFKRAPSKAPFYEIEDITRDLDFALKDSVRYGQKMPLILLMDNGSTEEDMPSL
                     KMTRIYDLPVMVVDHHHPDEVVDDYLIAHVNPYHVGGDYGITAGMLGTEIARMVNPAI
                     ESQIRHLPAVAGVGDRSEAPERARYLAVAAPEYSEDDCRDIALALDYEQFWLRFGDGR
                     EIIKDILNLSGNPERHERLVDLLVEEANRAIEEQVEAIMPHVERRVLENGARLFMLDV
                     ELFAHRFTFPPPGKTSGEVHDRLVRAHPGEPIVTIGYGPDFAVLRSRGVMMNIPQMVR
                     ELRNEITGAGVSGGGHLVVGSIKFVEGMRDVVLENLIKKIGKAPI"
     gene            29427..>29609
                     /locus_tag="PK196_01110"
     CDS             29427..>29609
                     /locus_tag="PK196_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HON40057.1"
                     /translation="MKSAILGGGLTGMTLARLLHERGGRVIVLEGNDKVGGLCRSRTE
                     AGFTFDIGGSHIIFSRD"
     assembly_gap    29610..29709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29710..30895
                     /locus_tag="PK196_01115"
     CDS             <29710..30895
                     /locus_tag="PK196_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON40058.1"
                     /translation="EAGFTFDIGGSHIIFSRDSEVLSFMLSVLGENADQRNRNTKILY
                     KGRYVKYPFENGLYQLPDEDRFFCINEFIKNLIAVEKGEVAPPTNFAEWINFTFGRGI
                     AECYMIPYNEKIWNYPINRMSHHWVEGRIPRPPVEDIIKSAIGIETEGYVHQAVFSYP
                     VRGGIEALIRAIAEPILPAVRTGFSVASIREENGGFAVSDGRETVYADRLISTIPLQH
                     LLPCLTDVPADVQAACGALRYNSLCSVFIGLAGEVPDISWLYVPDEKTGLFNRISFPS
                     NYSTAVAPPGHSSILAEITYNEGDAVSRMTDDEVIEHTVESLIAAGFIPSHDTVVYTG
                     IERQKFAYVVYDIDYLKNIAIVRDFCRERRIDLVGRFSQFEYLNMDGCIRSAIDFVEG
                     HP"
     gene            30892..31875
                     /locus_tag="PK196_01120"
     CDS             30892..31875
                     /locus_tag="PK196_01120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HON40059.1"
                     /translation="MKVNFFVEDMFFFKYIGCATLAKMLYSALIEDDEPCLQVAWNAV
                     NRDFDLVHYHTFGPLALANRAYSPGVKVLTAHSTPRLNIGNLAFFERLNRLYPEIYQG
                     FDHIISISPLCDEEVHEIAPDVPTTIIPNGVDREKFRPDPEKRAAFRERYGIGEDEWV
                     VLTVAQQTPRKGIYDFLALSHEHMDTRFVWVGGFPYGRFSQDYSIIEEKKSRCGKNVI
                     FTGFVDDITAAYCSADVFFMPSYAEGLPMVALEAFATGLPVVARKIPEFTGNFKEIAV
                     YFENLEEAGRLLEDRSLLEQHAALSRPFTEDYDIRKIAKLHINLYRELTGQ"
     gene            31979..32677
                     /locus_tag="PK196_01125"
     CDS             31979..32677
                     /locus_tag="PK196_01125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON40060.1"
                     /translation="MISVVVPTYNEEQNIERCLRSLADQTVPRKNYEIIVVDGNSKDR
                     TRDLAEPLADQVFIQTSKRVGGARNDGAMAASGDIIATTDADCIIPRDWVERIERNFS
                     AHDIVQLYGTVYPIEGGLRNRLSLLGANAFSRLGYYTRTIYFTLGCNTAFDREAFIRA
                     GMYRCIDAGDDLEIAQRMRKLGRVHLDPGLKVGFSMRRYQQFGTIRSLWEWFYIVRSG
                     GEAGDATYTKREYR"
BASE COUNT         6480 a         9537 c         9535 g         7053 t
ORIGIN      
        1 gtacggaact cgtcaaagtc gatccccgcg acacgacaca gatgtgttcg aactgtggaa
       61 gcatcgtgaa aaagacgctc tccgagagag tccacgaatg cccgtattgt gggtttgttg
      121 ccgatagaga ttacaatgct gcggtaaata tccaccgcgt ggggatggaa cagccctttg
      181 agcctgtgga gccaagacct ctacatcaca tctctgtggt gcaagtgttg gccatgaagc
      241 aggaagcccc gcccgagagg cgcggggtag ttcacatgag atggttggcg ctgttttaga
      301 tgcccggaga tctgatgatc ttgtcccttt gaaagtaacg gcggatctct acattgaagc
      361 agatgacggc agaagattct attttgagat aaagtccccc atgcccaata aggggcagtg
      421 tctggaggtc acacagaggt tattgcggtt ccatcttatt gcaggtgctg cccgacctga
      481 tgtccatgga tattttgcaa tggcatataa cccgtatggc ccgaaacgtg atgactacaa
      541 ctggaatttt gccttaaatt atacaccgtt tgctgaagcg gtactaatcg ctcaggaatt
      601 ctggagtatt gtgggcggcg aaggtacaat ggaggaactc ctctcgatct atcaggaggt
      661 tgggcgagag aagacaaaat acatgctgga ctctctggca tttggatttt gatcccggta
      721 tccggtggtt ccggcggtcc cgatggtgca gcaagagatc aagatagggg attgatcagg
      781 ggtgttgatc gtgaggccga atagacccgg caacccgaaa cagagaaatg gctgccgtgt
      841 tcgagagatc agccgcatag aaggggcaaa aacagcccta tgaacccggg cggagagatt
      901 gcgcatcggc cctgatgctg cagggattca ggtttccgtc tttgaggtgc atccctccaa
      961 tcgcagaaaa cgttattggt cttcgagtca ggatgtttta tctgtaccac gaatgccatg
     1021 agccctggag actggcaacc cggggcgtga ccgactctcc gccgataacc gtgtatccag
     1081 ggcagtgact ctcgtggaac ccgggaggtt cgcgttctct cccggagttc gccttttggg
     1141 agaagaccgc ggcgtgaagg cgcaacccgg caactggcgg aaaagatctt acgagtgctc
     1201 aataccgggc agggtgagag accactaaaa ccgttatacc agaacaaaga gggggaagta
     1261 atttgaagat acctgatgga gattgcaaaa ttgtcttgac cgcggatcgc actctcatga
     1321 gcgattatca tcataacgaa tttctggggt ttggaacatg cgccccacca aacgtcctcc
     1381 cgaactggtt ttataagtgg ctcttcttcc cgcccatcaa gacagagggc ggagtgccag
     1441 tcgctgcacc atatggtctg cggaagatcg aggccgaact ggcactggaa gggtttgatg
     1501 cggtggtggt cgatcccgac catctgggca aataccttga gaatgcaaaa gttctgagca
     1561 tatatgtcat ggaccctttc ggtctgggcc cggcatcaag cacctttgcc ttcttccttc
     1621 agaaagaacc gttcctggcc gaatatttcc gtaaattgat gagcagcaaa gagataaagg
     1681 aggcaaaaga gcgcggcctc aaggttatcc tcggcggccc cggcgtctgg cagttcaaat
     1741 ataggcctga gttcgccatg aagtatggga ttgactgcat aatcgatggc gaaggcgaga
     1801 aggtcattgg aaagattgtc cgggctgcac tgaacgacga gaacctccca gaatattatg
     1861 aggtacctcc tgaagaggtt ccatctctgg aagagatacc ggggatcatc gccccctcta
     1921 tcaacgagct cgttgagatc ggccgcgggt gttgcagagg ctgcaggttc tgcaacgtaa
     1981 ccctgaggcc gttgaggtgg tacccatacg aaaagatcct gaaagagatt gaggtaaacc
     2041 gaaagaccgg aaacggtgtg tgccttcatg ccgaggacgt gatgctttat ggctcaagga
     2101 acaccactcc agacaccgag aaactcacca gacttcacga gattgtctcg gataagtgcg
     2161 atgggatagg atggagccac tgctctcttg cggcggtcgc atcgagcccg aaactcttct
     2221 cgcaggttgc cgagatcata ctccagaaac agtcctggtg gggtgccgag gtcggcatag
     2281 agaccggttc cgcggaactg gcaaagaaga ttatgcctgc aaaagcccat ccattcaagg
     2341 ttgaggagtg gccggaagtc atccgcaccg ggatgggcct gatgcacgac aacatgctgg
     2401 tgccggcatg caccctgatc gtgggcgggc cggaggagac cgaggatgat ctcatcagaa
     2461 ccatagagat gatggacgtt ctcagggata tcagaagcct gatcgtgccc ttattcttcg
     2521 ttccgatggg caggttcagg aatgaggagt ggtttgatga gactgagatg aacggcctcc
     2581 accaggaact tctgttaagg tgtttgaggc atgacctcta ctgggcagac agcctgatcg
     2641 atctctcgat cgggcgagac tggcccggca aactcaggag ttccttctac aaactctttg
     2701 tagggctggt caaacggagg atagaacgcg aaggggtaga gatctgagcg caggcggcag
     2761 tccccgcgta ttgccctggt acgaggatgg acgtggtcgg tgatgcagtt gcagcgctga
     2821 tcagcggaga ttcaaatcga ttatgagcgg gcgcacccgg ttccagatat cttactggcg
     2881 gataggtgca gtgctggcag gtcacaggcg ccccggaaca aaaaaaagat caaatgaacc
     2941 ggggcttcct cgagaggcag cccggcgtgt agagcgggcg gaacggttta ccttcggtct
     3001 ccattctcac cgcctggatg agttcgctct cggtcatcgt ctcccggtac ggttgtttct
     3061 tctccgagag tctcctgatg gtgaccgtga gcctgccggt ctcggcctcc aggtcgccga
     3121 tcataacgac gtatggcacc cagtccaccc cggcctcccg gaccttcctg ttcacgctct
     3181 cgtccctgtc atcgacatcc gcccggatgc ctgccgcctt cagccggccg gctatatcct
     3241 cggcaaaaca gacgtgccgc tccgccaccg gcaccagccg cacctgggtc ggcgcaagcc
     3301 aggtcgggaa cgagggcacc tcctgggtcg cggtgttctc gagcatcgcg cagatcacac
     3361 gctcgatcga acccgttggc gaacagtgga ggattggggg atggatctct tgcccctcgg
     3421 gtgtgtagta actgatatcg aacctctccg aactctcgac atcgatctgg acagtcgggt
     3481 tctcgatcgg cctgccctgc gcatcgatcg ccgcaaggtc caccttcgcg atccagtaat
     3541 ggctccgctc agagaggata tcgatcagca tcggcacccc cgaatttgcg acgatcttct
     3601 tcacccaggg ttcatactgc tcaaagaagt cccgggtgca cctgaagacc ccgacaagcg
     3661 gcgtatcgag gtcgcggccg ctcttccagc ccatcgccag ttgctcctcg aacgccgcaa
     3721 gcgcatcatc catgtcccgg cagagtgtgt gcatatcagg catcgtgaag gcgcggagac
     3781 gttttagccc gataacttcg cccttctgct cgtggcggaa cgagtatgtc gagagctcgt
     3841 agagtttcat tggcagggtg tttggcgaga tgtgcatgct gttcatgatg gagaacatac
     3901 cgaaacacgc cgcaaaccgc agcatcatat cacggttgcc gctcttgaac cggtactgcc
     3961 gctccccgaa cctggcggcg tgctccgcaa tagccttatc tgcgaggtca tacatcaccg
     4021 gggtctccac cggcatcgca ccgtaatcga gcaccttcga gaggacataa tcggcaagaa
     4081 ggtcgcggat caacttgccc cggggcatcc agcggaggtg gccgacgtcc gagcgcggtt
     4141 cataatccac aagttccttc gcccgcatca gatcaacgtg ggccggctcg accccttcct
     4201 gcccagcata accgatctcc ttctggacaa ggcaggcaaa cggggtattt ccagccgcgt
     4261 agtcggcggc gtcccggcgt tcgccctcgg gtgtgatgac gaagaactca tgctcgatct
     4321 ccttctttgc ggcggccgca acccctcccc ccgggacgat cgtccttgag agctcggaca
     4381 gcggatgacc cttacaggag agagagaacg ccttatacca gccaaaaggc gcccttctca
     4441 caacaaaatc gtcctcctgc tcccggagcg cctcctcaat atcccggaga gcgctaaccg
     4501 ccacctccgg cgacgccagg tcggaggaga ggtgtgcgta ggggtagatc attatattgg
     4561 tcgtgccgag ttgcctggat gtgttgacga tctcctcgac cgcctgccgg acggcatcat
     4621 cgatattctc ttcgtcgaga gactctaccg cgcagaagac agcaagcgcc tcatcgagtc
     4681 cgtccttcag gacggcatcc tcttcggcgg ccttcgtctt cttccgtgcc tcatactcga
     4741 tatgatcaga gtggatcaga agaagtcgca ttggtatccc cagttgaatc gttcaatgtc
     4801 tgttttcctg ctatatagtg cataacgctc tctgacgtgg ccgcaccagc gcagcgaccc
     4861 gacctcgcgg cggcggcggc gtttttgccg tgtcgtcagc accctcatca ggtgcggcgc
     4921 gcgttctcat ggtagcggga gagcgtcgcc tgccgctggt tcatatactt tgcatccttc
     4981 ttcatgtagt aggggcgcgc ggcgcgatcg gtgtggtaga agaccagctg cccgatcgcc
     5041 atcccggcgt agacccgcac cggtctggag ttcacgttgc acatctcaag cgtgatcgtc
     5101 ccccggaacc ccgcatcgat ccagcctccc gtctggtgga gttcgacccc caggcgtgcg
     5161 atgctgctct tcccctcgat gcttgccacg atatcgtcgg gaaggtcgat cgactccagc
     5221 gtctcgccaa gaaggaactg gcccggcgca agtacaatag agtcggccac ggtctcctca
     5281 gtccccgcga agatggtctc cttctcaaaa ggatcgatca ccatatcccc cggaacgtac
     5341 cagacaaagt gcgacccaag cctgatatcc agcgagtttg gctggatcag gtcgggatca
     5401 tatggttcaa cctttatatg cccccgcctg atccggtcct ctatctgcca gtcgacaaga
     5461 atcattctat cacatctctc ctgcagacta ttccttgttt tcgagatgcc actccgcccg
     5521 ccgcatcaac ccgtgcagcg tgctcacctc acgggtcgtc agtttagtcc gaccaagcac
     5581 acggcggatg agtgtcagcg tgttctcccg cttgaaatcc ggatgttcga tccggtcgag
     5641 aaacgcgtct atgtgacggt agagcgactc catctcgacc ggcccggcca gcagatacgt
     5701 cccgcgcggc aggccggcaa gttcgtagca gaggatacca acagcgtggg agaggttaag
     5761 gatggggtag acctccgatg tggggatggt gcagatcagg ttggcgcgct cgagttcggc
     5821 gttcgagagt ccccagttct ccctcccaaa gaggatcgcg accgatccat cgatatcggc
     5881 gatgacctct ctgacctctg ccggcgtgta gtagggcatc cgcatcgggg tgcagacgga
     5941 tttgctcacc tcaccggttg tcgcaacaac aaagtcgaac tcccggtaga catcgtcgag
     6001 cgtcatccgc ctggcggaat caaggacgtc gcgagcgtga gatgcgcgca tgaaggcctc
     6061 gtcgccaagc gcgcaggggt tgaccagcac cagacggtcg aacccgaagt tcttcatcac
     6121 ccttgcggta aacccgacgt ttccctcata gaggggttcg accagaacta tagagatttc
     6181 tggcataatc ccttttcagt gcaccgcccg gacggtcgcc cggagcacct cgatcccttc
     6241 ctcgtagacc gcgtttccta caacgatcgt gtcggcgtac cggcccatct ccgctgcctg
     6301 ttcggccgaa cgtattcccc caccgtagta gaggagggca tccttgatcg caccggccgc
     6361 cgcccgcacg atctgcggat ccccgtatct cccgctgtac tcgatgtaga cgatcgggaa
     6421 cctgaagtaa cggtcagcta cctgtgcaaa cgccgccacc tccatcgcag agaggttgca
     6481 gtctgctccc gtcacccggc cgaccgccga gtcggggttg aggactatgt aggcctcggg
     6541 cacaaccata tcccaattta tgccatcgcc gttgttgagg agccaggtgt agtgcttccc
     6601 gacgatccag cggacgtcgt ttgtgttcag cacgctcggc acaaagagat ggtcgatccc
     6661 gccatcgaat atggcgcaat cggggctcgc cggttcgacc accaccggca gcccgtaggg
     6721 ggagacctgg tccaggagtt caaggaggtt atcccgtgtc acgttctgcg tgcccgagag
     6781 catcagggca tcggtgccgc ttgttgcgat ctcctcgatg gctccacccg ggaggcgctt
     6841 atcggggtca agttttgtca cgtgggtcca cgtcttccag ttggcgtgca ttgtctatca
     6901 cttcaggtgg tcgatgtagt catggatcct ggccttcggg atgccggtga tggaggagac
     6961 catctccggg tcagcctcca ggagtgtttt ggcatcgtag acccctgcaa ggtatagtct
     7021 ctgaagggtc gcctcgccca gccccgggac ccccaggagt tcttttcgtc cgcgttcgat
     7081 cacctgtctg gttatctttg gcggggtggg gcggccgaga tacctgcata ccaggtccac
     7141 atgtttatgt acggtttcag ggttaatgcc ggttttgcct gctaggtagg ggatgggaag
     7201 gtaacatagg tcctcgggac tgctgatgcc ggcctcaagg tacttcttca ggctgacaga
     7261 aggaaccccg gcttccctca aggcgcgttc gctggagagc cggcgcgctt catcgagcaa
     7321 ctcgtttgca gccgtttcgg ggatgcccgc tctcttaagt gttgcaacct cggcctgaga
     7381 gagagcgtgg atatcaccga tcccttcttc aagcaggttc ttgaagatcc tcacgtagct
     7441 ccgccctttc ttcgggggga ctatcttccg gacgaatttc cggagttccg acctccggcg
     7501 aagaatctca agcgcatctt cagcctcccc tgcaagccct ctcgcctcct cgatatcgac
     7561 acccaggacc tctgcgagtt cttctggtgg catgcctgca atatcggata cggtgtatat
     7621 gtgctgttcg tgcagacgct gcagcaggtc atcggtcatc gagggcatga ggaaaagcgc
     7681 ctcaatgttg gaggatatat ggctgcatag cgggcaaccg atgtcccagg ggcgggcgcc
     7741 tttcttgatc aggcggatgt gagcgaggtt gtgcttctca caggtctcat ccgtcctgat
     7801 ggccctgccc caggctgtgc cgggcaaact gatgttgaac cggcactccg ggtagccggt
     7861 gcaaccgata aactgggaaa cgccgctatg ccggatccgg agatcacggc cgcagacggg
     7921 gcaggggccg acggtatgct cctcagcggt ctgctccatg atctcgtttc cgatctccga
     7981 ctcgtgcgcc tcaagattgt caaagacctg gtgcagcatc tggcgcgact cggtgatgac
     8041 attgtcgcgg gagcgccggt tctgttttat gagctgcatg tgctcctcaa gcgtcctggt
     8101 catctccggc tcggtgatcg tcgcggcgtg gttgtcgagc gcgtctatga ccgccctgcc
     8161 gacaagcgtc ggccggagag ggttcccctc cacataccgc cttgagatca gtttcccgat
     8221 cacctcgtgc ctggtgctct ttgtgccgag tccgagttcc tccatcacct ggatgagccg
     8281 gctctgagag tagcgtgcgg ggggttgcgt ctctttctct tcgagtttca cgtctattat
     8341 aggcagccgt tcacccggct taacctcagg aaggatggtc tccttcgctt ccgagtatgg
     8401 gtagacccgg cgccatccct catcgatgag cctgccgccc gtggcgcggt aggtctcacc
     8461 agaagcatca aatgtatacc gggtggttgc ccatttcgcg tccggggaga gggttgccag
     8521 gaaccgtcgc acaaccagtt cgtagacctt ccaccgctcc tccccgagag cctcacggct
     8581 tgctgccgcg gtcgggtgga tgggggggtg gtcggtgctc tccttcttgc cgcgtgtagg
     8641 ggtgggacgc cggttctgct gcacccaggc gacgtcctta tcaaagaccc ctcccttcag
     8701 ggcctccagg ataccgttca ggttcagcga ccccgggtag accgtgttgt ccgtccgggg
     8761 ataggagatg taaccgttca tatagaggtc ctcagcgatc cgcatcgcgt ttgccgccga
     8821 gaacccgagt ctggccgccg cgactatgaa ggtcgtggta tcgaacggtg tcggcgcacg
     8881 gtcgaccttc tgcccctcct ccacgtccgt gacaacaaga ggctcctttg tcctctcctt
     8941 cgcggtgaat gccgcttcac gctcggtgaa ccggccggtg gtgtgtcggg cctcaaatgc
     9001 tgtcccgtcc ttctcggtcg tgagcgagag cacccagtat gtctggggca caaagttctc
     9061 gatctcgcgc tcccggtcga cgatcatggc aagcgtcggg ctctggacgc ggccgacgga
     9121 gaggatatcc ctgccgccac ggcgtgccgc aaggctgagg aaccgggtca gcgccgcccc
     9181 ccacatcagg tcgaccgttt gacgggcctc acctgcggcc gccaggttaa aatcgagttc
     9241 gactgggttt tcaaaggccg cccgtatctc cgccggggtt atcgccgaga acctggcgcg
     9301 gttgatcctg acgtcggggt tcaccgcacg cacaagttcg taggcctctt taccgatcag
     9361 ttcaccctcg gtatcgtaat cggtggcgat ggtgacgaga tcagcctttt tcgccagttt
     9421 ctggatgagg ttaactatct tcttctcggt tggtttcttg accatcccgg catcgatgag
     9481 acttcgcggt gtgtgggttg tgcttcgcca gtttgcgtag cccggttcaa agtccacctc
     9541 aaccacatgc cctttgaggc ccacaaccgt cctgttgtcg aacgagtagg tgaagacact
     9601 tccgtctttt gttgtcttta ccttctcgtt gcctgcaagg atctgcgcga tccgcttggc
     9661 cgagatgttc ttctctgcga ttataaggtg cactctgatt acacctcacc tttatcgatg
     9721 tattcagcca ccatgcgccc gagttcccgt gggtccgccc gaccgcgggt ctctttcatc
     9781 acctgtccga ccaggaagtt cagtgcgcct ttcttccccg cccggtagtc tgcaactgcc
     9841 tgcgggtttg cggcgaccac atccccgatg accactgcaa actcgtcccc ctcgcccctg
     9901 acaagtcctt cgcgtttgac tatcacggcg ggaggttcac acggccttcc agcggcgcag
     9961 tcatcaagca tttcacgcag cacctggacg ccgaccctgt cggtgatggt ccctgatctg
    10021 aggagtgcca ggagttctat gaatcggttg gcgggaacgt cggtgatccg catcccgcgg
    10081 tagttgagtt ctccaagcag cgtgtcggcc acccaggttg ctgcgaggaa gggatcgacg
    10141 taggcaactt cctcgtagaa atcagcaagt ctggggtcgc cggtcaacgt cctcgcgtgg
    10201 tttaacgata tcccgtactc tgccatgaac ctctctttcc gggcggtggg cagttcaggg
    10261 agatctatcc cgtcgaccca gtcagcgacc cggaacggtc ggagatctgg ttcggtgaag
    10321 tagcggtagt cctgctcccc ctccttgctg cgggcggatg tggttatccc ccgcgcttcc
    10381 atgaagtgcc gggtttctcg tccaaccttc tggcctcgcc gaaggaggtt cctctgccgg
    10441 gtcacctcga aggtgagggc cttctcgaca cccttgtagg atgagatgtt cttgacctca
    10501 acccgctcag agccttcaat cgagatgttt gcatcgacac ggagcgctcc ctcacgctcg
    10561 ccgtcgaaga cctcgaggta ctccaggatc gcccgtagtc tgttgagaaa ccggcgcgcc
    10621 tcctgagggg agcgtagatc gggttccgtg actatctcaa gcagcgggat gccggaacgg
    10681 ttgtagtcga cgagcgagta gccagaccct ccagcaacgt ggatgagcct tcccggatcc
    10741 tcttcgaggt gtatacggct gatgcggacg atcttttcat gaccctcgtc gtcctcgatc
    10801 tcgaccgtcc ccgcgaccgc gagcggtttg tcatactggg tgatctggta tcccttcggg
    10861 aggtcggggt agaagtagtt cttccgggca aactccgact cttccagcac ctccatgttg
    10921 agggcttttg ccaccttcag gccgtgttcg acagctctta tgttcagtct ggggagcgca
    10981 ccaggcagac cgagacagat ggggcaacag tgggtgttgg gctcgtcgtc ccggtagtct
    11041 gtcgagcagc cgcagaagag ttttgttgcg gtggataact ggacatgaac ctcaagccct
    11101 atgatcgtct tcatcgccct gccccctgct cgtaggcgta tgcggcatcg atgacccgct
    11161 cgtcttcaaa ccgtcttccc atcatctgga gaccaaccgg gagaccgtcc actctgccgg
    11221 cgcatggcac ggatatcgcc gggataccgg caaggtttgc cggcaccgtc aggatatcgg
    11281 ataggtagag tgagagcggg tcggtcttct cgccaagccg gaatgcaacg gtaggcattg
    11341 taggtccggc gattatatct gcgctgcgga acgcccgttc gaagtccagt ctgatgttcc
    11401 ggcgcgctat ctgtgctttt gcgtaatatc ggccggcgta gcctgcggag agggcaaatg
    11461 ttccgagcat gatccggcgc ctgacctcgg tgccgaactt ttcccgccgg atctccctgt
    11521 aggcctcgtg ccagggtttt ctgatatcgg ccgcagggcc gtagcgaata ccgtcgaacc
    11581 gggccaggtt cgaggaggcc tcgcatgtgc agattacgta gtaggcggcg agcgcatacc
    11641 gcatccccgg gatgctgacc gggactgtcc gggcacccag gtcctccaga acaccgatgg
    11701 cgtcccggac ggcctctgcg acgcgggcgt ccacaccttc accgaagtac tcttcgggta
    11761 tcccgacccg gagcccatca acattggctg atggctgatg atcgtagggg cggtcgatca
    11821 tcgttgagtc gcggggatcg tatcgtgcga tcacggaaag cagtcttgag acgtcctcca
    11881 ccgtccgggc catcggccct atctgttcga gggagttggc gtaggcgata agcccgtagc
    11941 gtgagaccct gctgtatgtg ggtttgaacc ccacgatacc gcagaaggct gcggggcacc
    12001 ggatcgagcc gccggtatcg gtgccaagcg ccatcgggac gagccctgcg gcgacggcgg
    12061 cggcgctccc gccggacgaa ccgccgggaa cacgcgaggt gtcgatgggg tttgtcgtgg
    12121 ggccgaaagc gctcgtctcg gtggttgtac ccataccgaa ctcgtccatg ttggtcttgc
    12181 caactatggc agctcccgcc cgtttgagga gttccacgac gtgggcgtca taaggtggga
    12241 tgtagccttc gaggatcctt gagccgcagg tagtctcgat gcccgctgtt gagatgttgt
    12301 ccttgacagc gatggcggtg ccggcgagca ccccatcaga gaacggcgct tcgctgcagg
    12361 tggtgatgaa ggcgttgtag cggtcgtccg gtgagaattc aagggttccc gccactcaca
    12421 tcaccctggg cgccttaata aacccgttct ccgtcgatcc tgcgttctcc agcacttttt
    12481 cctgcgggag cgatggccgg ggctcatcct cccgaaagac gttcaccgtc ccgctctcag
    12541 gggcgctttc gccctctaag gtatcgagaa cctcaaaata ttccagaatc gcgttaaact
    12601 ggctcgtgaa ctccgagatc tcctcttttg agataccgat gtccgcaagt tctgctatat
    12661 gctctatatc tctctcagtg atcatgttcc gccctgctga tctcagttaa gtaccgttgt
    12721 atcgattttt tataaccatt gtcacgtgcg aggttttgga tggtcctgta gatcccgctc
    12781 ccgtattgca tcgctttctt ctccgtccag gcattttcgg cgggaatgtg gcgctctgcg
    12841 atcacgcgaa gggggtattt tctcacacca ccgcgcaccc tcatcgatgc cgggatggtc
    12901 tgcgcggcgc gcaccacccg gagatcctgg tagggcatcg agaagtatgc accatgaagc
    12961 gctgcaaccg cctgatcccg cgcccgctgg cgcgcaaggt ctgcgaagtc gcgttcaagt
    13021 tccgccgcaa ggtcagtgga ggagaggtag cgggcgtagc cgccgaagat ctcgtccgcc
    13081 ccctgtccga ccaggatccg ggtatgcccc tgctcttttg ccagggctgc aacaaagtat
    13141 aatgttgtcg cgatcgaggc gtcgaccggg tttatctggc cggggatgac ccggacgacc
    13201 cggaaaaggg cgtcagcaac ctcatccttt ccaggcgata tgaccgtgag gtcgagatcc
    13261 atcatcctgg cggcttttgt tgcatgcgcg aggtcgtgcg accctgggag ccccacggtc
    13321 acgcagggga ggcgggcaag gtgggcgatt agagcggagt cgaccccgcc ggagaacgcc
    13381 acaacgcctt catcgctccg gagtccgatt gcagtaacga tcgcctcctc cagactgcag
    13441 gaagggggat ccgggtttat ccggccgacc ggccgccccc cgcagcagat ggttcccggt
    13501 ggggcagggc cgggcatgat accaaggttg tcccgcgcgg tgcagtcatc ccactcgatg
    13561 aggaactcgc cgccgcagcg ggcaaggctc tccgggtttg cggcaagcat ccgctccagt
    13621 tcggcagggg taagccgcac cccgccgtgt tcgatccagc ccttaagtct catacagttg
    13681 gtctccgggg gctggtgatc tgcgatctct gcgcacgatg ccgcccggaa gattctgatc
    13741 gcgtaggacg aggtttgaaa taatggttgc tgtggttttc atcgatgtcg tcttgaaggc
    13801 gccgcgcaag aaccataccg ccatgggtgc tgggagcagg agggggagag attcgcgaga
    13861 tgtcacttcc cgtagaacct cccttgcggc ctggctgttt aaggggaaat gggccttccc
    13921 cctgcatgaa agcgccgata gatgccgtgc tccgggtgca gcacaagatt tcaggagagt
    13981 ggctcaaaaa attactgagg gatatcttcc catcggtctt taaactcttt ctcgacaccc
    14041 gggagggtgg tatactccat ctcgcttaga gagagtcggt gcggttcgaa gggtccgtga
    14101 gccctgatca tattggagag ttcacagcac tgcaggcgga caccgtcaaa cgccgggtcg
    14161 tcgaacatat ccgcgggccc gatcagcctg ccgttgcaga cctggaaccc gagacagatg
    14221 acccgtggcg gtccatcgaa ctgtgtgcag tttgcatcac agacgcccac cggcatgaag
    14281 gggccatggt gcgagccgcg catccagccc gccacaagga ttggcgtccg gaatgggtcg
    14341 aggacctcgc ccacagccgg gagcccgctc tgtgccctga tgatcatgac agggtcgtcc
    14401 ttgccgacat agcgcccggc aatcagactc agccgctggg tgctggtgga agccgcgata
    14461 agcccgttct tcttccagac atacttgata acgtagcggc tcggtgcccc gatataggcc
    14521 aggaggctgt aggactcctc gggtgtcctg aactggatcc tcctcttctt catgacatca
    14581 tgaacctcga aaatgaaccc atcatgcatt gatgggtcga tgacaagacc ggatgtgttg
    14641 aaggggtcgg caaagatctt gtagaggaag tagttccagg ctcccggctc tgtcttatcg
    14701 gccatgaaga tcaggacagg gtcggatcca cgctcctcaa actccatctc ggcaacacct
    14761 ggccccatac ccttgacgtt gccgctgaac gaatcgctga gcaggtcctg cccggcgccg
    14821 tagagtttca tctccctggc aatcccggca cactccataa agatatccca ggccagcctg
    14881 tggatctctt cgtcgtcctc acccctgtta tgagtcatga taagctccag gtcgtcaccg
    14941 cagtgggtga cataggagtc gatgatgagg ttaccttctg cctctttgag ccgccgggcg
    15001 gctgtctcga ggatcttcgg atgggttcgg gaatgtcccg gaaaactgcc aacgtctgct
    15061 ttaatcacgg atacagtagt cttaaccata atcttcacca ctggttgagc gggccggatg
    15121 cccttatgag tagttgatgg tgtattactg tatatgtagg tgttgttgcc tcaaaaatag
    15181 gttgctcgtg gggctcctac ctcaccggtt gtttcagcgg gggcagacag actatggcgg
    15241 ctctggatta atcgatcctg atcggtctgc cccttgaaaa cctgacctct agaacgccgt
    15301 gcctgtatct tgcctccatc gagtccggat ccaccggcgg cagatcgatc gaggctgcct
    15361 ggttctcacc tgcaatgatc gtcagcgtgc cgttgatgaa caacagccgg atctcatgct
    15421 cctcaaatcc aggaaggtcg gctgcaaccg tcacatcgtt atcggtcgtg tagatctcga
    15481 ctgttggttc catgttcatc ccgacggttt caacctcttc atccggagga tcaatccttc
    15541 ccctgcctgg ctcttgccga cgtcctgaac gccgatcctg aaaccgcagg caatcgtcct
    15601 gcccggttcc tcgtgctcca tgaggcgtct tatcagtgcg ttgatcagtt caaaggtgtc
    15661 tcgctcacct caatcgcatc tgacggcggg ggccctgctg gacccccgga tagaaggcct
    15721 cacgggctgc tggggatccc tcctcagaag gccgtgccca tctgagccat cggacgttcc
    15781 aacctgctct tcaagtcctc tgtgaggcgc ttgatctcgc caaagtcctt cttccttgcg
    15841 tataccgcat ttttgagggc gcttgtgaga tctttgactt cctgttcggt cttgccggtc
    15901 tcctgcagcc tggcacgttt gatcagcgag ctggcctcct cgagcagcga ccgctgctcc
    15961 tggttgtaga ggaacttcca ggcctggcgc tcgtaatctt ccaggcgttc gagatcgagc
    16021 gtgcccttca cgcggccaag ggcctcctcg aagtgcttct tcgtgatcct gacgttgccg
    16081 atcgcctgac gccgctcctc ctcactcttt tcgcccattg cagtgatgaa ctcgcgcatt
    16141 gcggccagtt tcgcctcgcg gacgagcgcc tctatgtcag caccgacata gccctccgtc
    16201 ctatcgacca gctcgtcgat gttgacgtct tttgccagta tatccttgtt ccggaggtag
    16261 acctcaaaga tcttcctcct gctctccctg tctggcggcg ggacgtatat gaccctgtca
    16321 agcctgccgg ggcgaagcag cgcctcatcc agcatgtccg ggcggttggt agcgccaaga
    16381 accatgacgt tcttgagctc cacaagaccg tcgagctccg tcaggatctg gcttaccacg
    16441 ctctcggtca catgggatga gcccatataa gttccgcgtt tcggaaccag agcatcaatc
    16501 tcgtcgaaga atatgatcga cggtgccgct tgccgtgctt tccggaatat ctgacgcaca
    16561 ccgcgctccg attccccgac ccacttcgag aggagttccg ggcctttgac agagataaag
    16621 ttgcactcac tctcgtttgc aaccgctttt gcaagcaggg tcttacccgt gcccggcgga
    16681 ccgtagagga ggatgccacg tggcggtttg gtgtccaggg atgagaagac ttcagggtac
    16741 ttgagcggcc actcaatggc ttcagaaagc tcctgcttga catcttcgag gcctccaaca
    16801 tcctcccact tgacgtcagg gacctcaacg agcacctcac gcatagcgct tggctcaacg
    16861 tgcttgtgcg cctcgatgaa atcctcgttt gtcacgcgga gctgctcgat gatctcggcc
    16921 gggatctcct cctcgatctt gatctggggg atgacacggc ggagagcgtg catcgctgcc
    16981 tcctttgcaa gcagagcgat atcggcacct acgaaaccgt gtgtggttct ggcatactcc
    17041 tcgagatcca cgtcatctgc aagcggcatg ccgcgtgtgt ggatctggaa gatctgcagc
    17101 ctgcccttcg tgtccgggat cccgatctcg atctcacggt cgaaccggcc gccgcgcctg
    17161 agtgcgggat cgatcaggtc aggcaggtta gttgcggcga tcaccaccac ctggccgcgg
    17221 gacttcaacc cgtccatcag tgcgaggagc tgggcaacga tccgccgctc cacttctcct
    17281 tttacctctt cacgcttggg tgcgatcgca tcgatctcat caatgaagat gatcgaaggc
    17341 gcgttctcct gtgcctcttc aaagacctca cgcaggcgct cctccgactc gccgtagtac
    17401 ttgctcatga tctcggggcc cgagagggtg atgaagtgcg catccacctc gtttgccacc
    17461 gcttttgcga tcagtgtctt ccctgtgcca ggcgggccgt agaggagaac cccctttgga
    17521 ggctcgatcc cgagacgctc gaagagttcg gggtgacgca gcgggagctc gatcatctcg
    17581 cggacgagct gcagctcgcg gtccagacca ccgatgtcct cgtagtggat gtcgcccgca
    17641 atctcacgac cgcgttttgg tgccttcgcg ggctcgtagg gtgtctcctt cagttcgatc
    17701 tcggtgctat cggtgacgat ggccacgccc ttcggtaata ccttggcgac cgcgaacgtg
    17761 agcgggttgc ccaggatgtt gatccggatg agctgcccct cggtgaccgg acggccgcgg
    17821 aggagccgcc cgaggtactg ctcgccgcca accaggcgta tgggctgcgt gggctggatg
    17881 acgaccttct ttgcgtatcc tgcctcaatc tttctgatcc gaaccttgtc gtcgatgccg
    17941 gccccgacgt tgccgcgggt gcttccatcg atacgaagga ccgcctttcc cgtatcctgg
    18001 gggaaacctg gccagaccag cgtcgccgct ttctggcgcc cctcgatctc gataacatcg
    18061 ccgctgacga gattgagcgc cttcatggcg tcaagactca gcctggcaat tccccggcct
    18121 acatcctcgt gagccgcctc tttgactgta acttcaatgg aattattatt ggtcattgta
    18181 attgactcct atctctttgc ttaccatagg taagtattaa caagtatata tacatttcca
    18241 ttaggtgccc atgtcactga cttctataat cactttttcc gtgataatgc agtccatcat
    18301 tatatcattt tcgtcagcag ggatgcactc cacctgctgg caggagaagg cgatgccgat
    18361 cttttctggg tgagggtatc gccggaggaa acgatcgtag tagcctgcac cgtatccaag
    18421 gcggttcccc ttagaatcga aaccaagcat cgggacaacg actgcctgga tgtcttccgg
    18481 ccttaccggg atctcgtggc cgatcggttc ggggacgttg aacgtgctct ggacgaggac
    18541 acccggatct gggagggcag agagacggag gctgcgggtt tccctctcta tgataggcac
    18601 aacgaccagg acgccccggt tgttcagggc cgcgatcaac ccgcgggtct cgacctccgg
    18661 gggttttgag acgtagacca tgacggttgt gaacccgtca agaacctcga taagcctgcg
    18721 ctcgatgctg gcgctgtatc tggcgatctc tgcaggggtg agacgggatc ttgcctcctt
    18781 tgccctcata cggagttcag cctttgtaag ccgcattaaa ggaatgtttg gcagaatcag
    18841 gccttaaaat atgccccggt gagcggaaaa actggaagac aactctcata caccgggttt
    18901 tcggcctcct gcagccaggc ggtggaggac gttctgcaaa agaagttttc gaggagatgg
    18961 aagagacagg ggcgtgggat gttccatccc ccgccccgtc agcccctccc ccacggggcg
    19021 atgtcccatc gatacagtgc cacggacagg atgagcagat ccgcggctcg atgtgaacac
    19081 cgtgccggaa tcctgctttc agccctatgc agttgtatcc gggctatcat cactcactgc
    19141 ccgccccctc ggaagggggc gggaggaggc cgcgcgagcg gccgggttgt gggggtggtg
    19201 gtctacctca cacggaaaag agcgtctggt tgagatggag gagacagggg acgtggatgt
    19261 tccatcccct ccccctcagc cctaccccca actgtggcga ttctccatcg atgcagtcca
    19321 gaggaggtgc atcccctcac ccggctcctg gtgagcgact tccataaaaa acctgtttcc
    19381 tccccggaca ggtgttgtcg aaggcacggg tgacagtccg tccgtctact cccgcccgcc
    19441 tcccccctca ccacagacat ccctgaactg gcgtagggtg gtggcaagga tgcgtgtctt
    19501 gcctatcatt ccggcgatca ggagggcatc caggatctgt tccggcgaag caccctcctt
    19561 cagcgcggcc cccatgtgaa ccttcaggca actctccgca cctgcgcctg ccgcggccgc
    19621 aacggcgacc agttccgcgg tcttctggtc gagggaatca ggccgggaga tccgataatc
    19681 ggcaagagcg gagagcacaa agacctcagg gcgtttgcgc agcgcaccga agatgaaagg
    19741 cacaaccccg agcatctccc ggacatcctc tgtgatctca tcgctgaggt cgtcggtctg
    19801 agcaaggaaa tcttctacaa gtttttcggt ctcaggtttc atgatgtatc ctttcccctg
    19861 gctgaaaata attggcacgg atggcatcca gcgccagtct atcagaagtt gaagtgccgc
    19921 ccgacaatct ttaacgcaca gtagtccccg cacatcgtgc atgcatcggc atcggcgggc
    19981 atcctctcct gccggatcgc cctggcacgt tcggggttga tcgcgaccgc aaactgccgc
    20041 tcccagtcga ggtcgcggcg tgcatgcccc atctccaggt cggcgtcacg cttcttgagt
    20101 ttaaccatat ccccgacgtg ggcggcgata cgtgcactga tgacaccctc gtagacctcc
    20161 tcgggtgtgg gtagcgccag gtgctctgcg ggcgtcacat agcagataaa gtcagcacca
    20221 tatgacgagg agagagcggc acctatggcc gccacacggt cgtcatatcc aggcgcgaca
    20281 tcggtgacca ggggacccag catatagaac ggttttcggt tcgtaaccct cttctcgatt
    20341 atgacgtttg cctcgatctc gtcgatcggg atatgccccg ggccctcaat gatcgactgc
    20401 acacccgccg cgtgcgcctt atcggcaagt tccgcgttta tgagcagttc ctggatctgg
    20461 gcgcgatcgg ttgcgtcgtg aaccgcacct gcccgcatac cgttgccgaa agatagcgtg
    20521 acctcatgct ccttgaggat ctcaagaagg tagtcaaact ccgcatagag cgggttctcc
    20581 ttctcgttgt ggagcatcca ggcggtcatg aacgccccgc cccgtgagac aagcccgccg
    20641 tgccgcccct ggttctgcag gcgtttcatc gtctcgtagt tgatccccgt atggatcgcc
    20701 atgaagttgg tcccggcctt cgcctgctcc gccgtgatcc ggaagaggtc atcctcctcc
    20761 atgttaacga ccgccccata cttcctggcg gcctcgatga aagcctggta gagcggcacc
    20821 gagcccaccg agaggctggt cgcctcgatg acacggcgcc ggatctccgt aagatccccg
    20881 ccggtcgaga gttccatcag tgtatcggca ccggcgcgtt cggcctgccg tgccttttcc
    20941 atctccatat cgatatcgac gatgtccgat gacgtgccta tactcgcgtt gacctttgtg
    21001 cgaagcccct cgccgatacc gcagatcttc accttccggt agggggagac cgggatgact
    21061 atctgcccct cggccacacc gcgcctgaca aaatcttcgg ttacaccctc cgctctcgca
    21121 acggtgcgca tctcttcagt gatgacgccg cgccgggcat cttctatgag acccatgaac
    21181 ctatattgga tgaaataagg tgataaagcc ttgttttttt gaaatcgggc taaaatggac
    21241 ttgatcgctc ggcaagtaaa cttgatttga gattattcag atgttatttc gtagccggaa
    21301 acccaaggat tacaagaaat aagcacccac ccctctcttg caacgtgatc agatgcatgt
    21361 aaacttcgga ggatttgtcc ccctaagcac cgttgactgg cacggaaggg cggcatgcac
    21421 cgtcttcctg agaggatgcc ccgcgcgctg cttctactgc cacaacatct ccatccagga
    21481 cggtgaagac ctccgcgacg ccgacgagat agtcgagatg atccagggtt cacgcatggc
    21541 tgcaggtgcc gttgtcttct ccggtggcga accgactatg cagggaccgg cgctcgccta
    21601 ccttgcctcc gctgcccgca gaatgggttt ctccgtaggg ctgcacacaa acggggtctt
    21661 cccccgcgtg atcgagaaac tcatagaaga acggctggtg gacatgatcg cgcttgacat
    21721 caagacgacg tgggaccgct acgacgacct gctgaaactc cccgccgcaa atgcggtgag
    21781 acaatccctc gccgcatgca aacgggcaaa ggccacaggc agccttgaga cctgcgagat
    21841 cgtggtcacc ctcttccggg gccacgaggc cgaactcccg gagattgcaa aggctgccgg
    21901 cgacctcgac cttgtcctcc agcagggcgt gaccgctggt tttgtcccat tcagccacca
    21961 ggaactcaag accctggctg cgccgctcgg ccggcggttg cgtatccgga cacgggaaga
    22021 gggggaggtc gtctacgacc cgtcgcgata accgacgtta agcataatat cgtctggaac
    22081 gcaaccgtta aggagacgtt tatggcggtc gcggcaacct gcaccgccgg ggcccggcaa
    22141 ccggcttcct gccggcacgg cccgctgttc tggatgcatg gaggaaccgg atgaaggtca
    22201 ttggaatcgt tggtatgccc gggagcggga agggagaggt ctcagggatt gcccgcgaac
    22261 tcgggatacc tgtcgtggtc atgggggacg tgatacgtga acgggcaaga gaagccgggc
    22321 tcccccctac agacgaaaac cttggtgcga tctcggcgca gctgcgcacc gacctcggca
    22381 tggatgcaat cgcccgtctc acaatcccgg tcattgagtc acagggcgct ccggtggtgc
    22441 tggtggacgg tatccggggt gaccgggagg ttgcggtctt ccgggagcat tttccggatt
    22501 tcacgctcat cgcgatcgac tcgagttttg agacgcgtta ccggcgcctc gcgaaacgcg
    22561 gcaggccgga tgataccctc acgccggatg aactgcgcgc ccgtgacgag cgggaacttg
    22621 gctgggggct tggtcgggca cttgaactcg cggactatcg gatagaaaat gagggttcgc
    22681 ttgaagagtt taccgcagag gtccgcggcc tgctatcccg gctggcaggg gtggacggat
    22741 gagtctcatc ccgtatttct ccgcctcctc aaaagtctgg gagtcatttg actggatata
    22801 tggggtcgag gagatcgggt acgccggctg ggagatagtc gctgacggga actaccgcct
    22861 ggataaccct gcgaatttca ctgctataaa ggaatgtctc gcgagcaccg ggcttcttgt
    22921 gagcgttcac gctccttaca gtgatttaaa cctcgcctcg ctcaaccacc ccatctggcg
    22981 cgagtccatc cgccagatct gctgctgcat ccgctatgcg gctgaactga ccgaccgggt
    23041 gacgattcat cccggatatc tttcacccct gggaaaactc gcccccgata tggtctggag
    23101 acagcagaaa gccgcgcttg atgagatcgg aaaagaagca gaggattgcg gcgtcctagc
    23161 atgcgtcgag aacatgatca gcatcagaga ctttctctgc cggtaccccg aggagatcct
    23221 cggtcttacg gaaggtattc ccgggatcgg gatcaccttc gatctcgggc acgccaacac
    23281 caacggtctt gtggacgcgt tccttgcgca tatcggggag gctgaccacc tccacatcca
    23341 cgacaaccac gggcagtcgg acgaacacct ggcgctcggt gacggcacca taccctggca
    23401 gaaggctggc cggaccgtcg cccgtgatta taccggcccg gtcgtcatcg agggccggaa
    23461 tctcgatgag gcgagacgaa gccttgcggc attcaggaga tggtttgtat agccggagag
    23521 acgcttcacg tccatttcct gggcacctct ggtgccctcc cctccccgca gaggaaccct
    23581 tcctgtatac tcatacggcg cggctccgac acactgctct ttgactgcgg cgagggcgcc
    23641 cagcagcaga tgatgcgcgc caggacaggt ttcaccgttg atgccatctt catcacccac
    23701 tggcacgccg accacttcct tggcgtattc gggctcgttg agacgcttgc gttcacagga
    23761 aggacagaac ctctcccggt gtatggcccg ccaggggttg gtgagtttgt tgacgtggtg
    23821 cagaggatcg gccgccacac ccggagtttc ccggtcaccg cccacaccct tgaacacggt
    23881 tcgattgtcc cattcaacgg ctacaccgtt agggcgtttg caacacttca tggaatcccg
    23941 gggctcggct atctcctcga ggaggacgaa cggcccggga ggttcaaccg cgaccgggcg
    24001 atcgagctcg gcgtcccgcc cggcccgctc ttcgggcggc tgcagcgcgg tgagaccgtt
    24061 cgcatcgtcc gtggcggtgt ggagacggag gtctcgcctg cagaggttct gggcgaaccg
    24121 cggcccggga ggaaagttgt ctataccggt gatacgcggc cgctccatga gagtctcgat
    24181 accgcggcaa tgctccggga tgcggatctc ctgatccacg acgccacatt cgacgaccag
    24241 gaacgcgagc gcgcccgtga ggttctgcac tcgaccgccg gtgaggcggg agaagcggcc
    24301 gatgccctgg gcgcccatat gctcgcgctg gtgcatataa gttccaggta tacgacaaca
    24361 acaaaccata tacgcgatgc ccggcgacag tataaaggca atgtgatcct cccggccgat
    24421 ctgacggtgc tggagatacc ctacaggagt tgatcgagat ggatggtgat actgatggag
    24481 caggggctgt ttgccaggag ccgcctgata cttacaggtc tagccgctct gcttgtcagt
    24541 gccgctgtct cgagcgtagc aggcatatat cttggttcag tacgtgaaaa cctcctgttg
    24601 ataccgggac tgatggtgct cctgccctcg atcatccata tccgcgggag catcgccggc
    24661 gttcttgcct cccgcctctc atctgcgatg cacctcgggg agttctccgt cgattttggg
    24721 gaggggagcg ttctcgggga caacatccgt gtctcctttg tggtgacaat cctgatagca
    24781 ttcgtcctcg gcatctttgc ctatgtggcg agccggatct ttgggtatcc ggtgatcggc
    24841 gtcacagacc ttgtactgat ctcggttgtt accggcatac ttgccgggac ggtggtgacc
    24901 gggatcaccc tgctcatcgc ccttgcaagt taccgctacg gccttgacct ggacatgatc
    24961 gccgcaccga ccgtcaccac ctttggggat atcataaccc ttcccatcct gatgatctcg
    25021 gcggcctttg tcatgctcct tgaccccctg gcacgcctga ttctcggtgg tgtagcggtt
    25081 ggtctcacgg tcgctgccat aatctacact ctgcaccggc cggaagggat cgggccgatt
    25141 gtccgggaaa atatcgggct gctcataccg ctgagcgtca tcggcacatt ggcggggctg
    25201 acctactcct ccagcctcga tatactggtg acaactgcag ccttcctcat ccttataccg
    25261 ccattcaccg gcattctcgg gtcgatcggt ggcatccttg gatcacgcct ctcgaccggg
    25321 atgcacaccg gtgagatcaa cccaacgttt atacccgaag gcaacgttgc ccatcatttt
    25381 attatttcct atctctatac tttgatattg cttccactgc tggcgctcat agcgcatggc
    25441 gcagcggtgc ttatggggtc aaatagcccc ggtccagtaa tgctggtaat catcagcctt
    25501 gcggcgggtc tgatcgtcat gacactggtg atcggcgtgg cgtacatcac cgcgagtctt
    25561 tcattccgtt acggtctcga tcccgacaac ttcgggattc ctgtcatcac gagtctgatt
    25621 gacctgatag gtgcagccgc actggtggca atgattgacc tgctgctgta gtcaaaaaaa
    25681 agaatccaat ttaacagacc atgactgacg ttgaatatca gccggttagt ttcaaagaag
    25741 tcctgatcga gatgaaggac atctctgagt tgatggttga cctggcttac tccgcgatcc
    25801 tattcgagag taaagagatt gcgctcgagg tgatcaatct tgaggagagg atgaatggcc
    25861 ttgtctatca ggccaggatc cagagtgtcc tcggcgcaag aaggcttgaa gaggccgagg
    25921 ctatgagtgg tatgctccag gtggtcgaag ccgcagagag gatcgctaac tcggcctcgg
    25981 atatggcaaa actcatctta aaggatatca agttccctgc agaactcaag cgcgccatgc
    26041 cggcggcgga ggagatcgtc accaggctca ttgtcagcca ggggagcgac cttgccggcc
    26101 ggacgctcgg cgacctcaag gtccagagca cgaccgggat gcaggtgatc gccatccggc
    26161 gtggaaaagg ctggatctac gatccagaca agaacacacg agttgaggca ggcgatatcc
    26221 tgatagcacg cggccctgag gccgggaagg agattcttgc ccggatggcc gggatggtgg
    26281 agtgtcccgg aaaagagccg tcgagcattg gtgaggtcga cgacctcgat cgcgcggttc
    26341 aactgatcat cgagatgaag aacctctcgg aactctctgt cggactggcc tacacctccc
    26401 ttctattcaa caacaaggag gtggcgcacg aggtggtctc cctggattca aggctcgatg
    26461 atatgcgcta cgacctggac ctctggattc tcgaggctgc acgaaagatc gatgacgtgc
    26521 gccacctgcg cggccttctc tacatgtcag cgtttacgca ggcgatcagc gatgcagccc
    26581 attcgatagt ggatgtgctg cttcgcgata tcgagatccc gccgctcttc aagaggatcg
    26641 tccgggaatc cgatgagatc atcacgcgga tgcgcgtgga gccgtcgtcg ccgcttgtgg
    26701 gcaggactct aaaggaggca tcgctcggga cggtgaccgg gatggttgtg ctcgccatca
    26761 agcgcggtga tcactgggta taccgcccgg gaaagagtgt tcggctggag agcggggata
    26821 ttatcattgc gaagggcagg cgggatggtg agtgccggtt gtatgaactt accgggcaca
    26881 ccgatgatgt gtccggggat tgatgtggag aactgaccat gcaaaatacc gatgatacaa
    26941 ttgtttatcg ccttggagca acctgtggcc ttgatgaggt ggaggaggga aagatctacc
    27001 tcgggtgggt acaggggttt gcccctttcg gcgtctttgt ccagttaaat gacagggtca
    27061 aaggtcttgt tcataagacc aacgtgaaga tgaagcatac cgagcgcgac cccatcatag
    27121 ttcatgtcct tcaggtcagg agcaatggca acatcgacct tgaggaggtc acgccgatcg
    27181 tctaccagac ggagaatgtg atcaagaaga tgccctctgt gctccttggc gatatcggga
    27241 agagggtcgg aaaaacggtg atgatcaagg gggaggttgc ccagatcaag cagacctcag
    27301 gcccgactat cttcacgatt gtggatgagt ccggcacggc gaacgcagcg gctttcatcg
    27361 aggccggagt ccgtgcctat cctgagatcg aactcgggga cattgttgca gtcaccggcg
    27421 aggtgatgca gcgcaacaac cagctccaga tcgaggtcga gtccatgatc gtcctgatgc
    27481 ctgaggaggc ggcagtggtc agggagcgga tcgaggcagc acttgataaa cgtgcggagc
    27541 cccgggagat ccagttcctg gttgagagcg aggttcttga ggccctgagg ccgcagatgc
    27601 gcaaagtggc aaaggagatc cgcaaagcgg tcttctcgtc gcgtccgatc gtcctgcggc
    27661 accacgcaga tgcggacggt atatgcgccg ccgtcgccat tgagcaggcg gtgaccgcac
    27721 tgctccggga gagcggcgct gattttgatg ctgagtattt cctcttcaaa cgtgcaccct
    27781 cgaaagctcc gttctatgag atcgaggaca tcacgcggga tctcgacttt gccctcaaag
    27841 acagcgtccg ctacggtcag aagatgccgc tgatccttct gatggacaac ggttcaaccg
    27901 aggaggatat gccgtcgctg aagatgacaa ggatctacga tctcccggtg atggtcgtgg
    27961 atcaccacca ccccgatgag gtcgtggacg actacctgat agcgcacgtc aacccctacc
    28021 atgttggcgg ggattacggg atcacggcag ggatgctggg gacggagatc gcacgtatgg
    28081 tcaacccggc gattgagagc cagatccggc acctgccggc ggttgcggga gtcggcgacc
    28141 ggagcgaggc tccggagcgc gcgcgatacc ttgcggttgc tgcgccggaa tattcggagg
    28201 acgattgccg ggatattgcg ctcgctctcg actacgagca gttctggctc aggtttggtg
    28261 atggcaggga gatcatcaaa gatatcctga acctctcggg caaccctgag cgccacgaac
    28321 gactcgttga tctcctggtc gaggaggcga accgcgcgat tgaggagcag gtggaggcca
    28381 tcatgccgca cgtcgagaga cgggtgctcg agaacggtgc ccgcctcttc atgctcgacg
    28441 tggaactttt cgctcaccgg ttcaccttcc cgccgcccgg aaagacctcc ggtgaggtgc
    28501 acgaccggct ggtccgggcg caccccggcg aaccgatcgt cacgataggc tacggcccgg
    28561 actttgctgt cctgcgttca cgtggtgtta tgatgaacat cccgcagatg gtgcgtgaac
    28621 tcaggaacga gatcaccggt gccggtgtga gcggaggggg tcaccttgtt gtcgggagca
    28681 tcaagtttgt tgagggaatg cgcgatgttg tgcttgagaa cctgatcaaa aagatcggta
    28741 aagcgccaat ctgatcaccc ttctttcctg ttgtttctgc ggtgttgggg ggcacacccc
    28801 tgaaaaccga tgccgcgttg ctcaggtggt cttttgcctg actcctgttt tgtggcaggg
    28861 cgcacctcaa aggtgtaaaa cacctctcag cgccacaatg agacccgtgc tccaggagag
    28921 gtggtctcgt tagtgctcaa cctgatgcag attatgtgta accgttgctg ctccccaccc
    28981 gctccacggg gagcgttgaa ccgccagggg tagaacacgg tcgaggaagg gcatgcagca
    29041 ggaggtacaa agagatagaa gtggggtggt cgcctcccgc atagaaagaa tagaaagacg
    29101 ttcgacagat agtatatgcg gagacgggga gtggaaacga cccgtctccc ctccgcgtaa
    29161 tcttcacccc tgcggccgaa tcttcgccga tacactttgt ccgggaggca ttttctccgg
    29221 catcacgttg aaccctcagt aaaaaccatc acatttccgt ccctgagcac ggcctctggc
    29281 agccatcacc accccatgcc cggctggaga ggatgggggt ctacgagagg cgctaccatg
    29341 tcccaaaatc caggatcccc gatctcttct gcgtatgcag cgttgatgct caatcatata
    29401 tggcactgat aagaaacaat atagacgtga aatcagcgat actgggcggc ggccttaccg
    29461 gtatgaccct ggcgcgtctg ctccacgaac ggggcggcag ggtcatcgtt cttgagggaa
    29521 acgataaggt tggcgggctt tgccgctcaa ggacagaggc ggggttcacg ttcgatatcg
    29581 ggggatcaca cataatcttc tcccgcgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29701 nnnnnnnnna gaggcggggt tcacgttcga tatcggggga tcacacataa tcttctcccg
    29761 cgacagtgag gttctctcct tcatgctctc cgtccttggt gagaacgccg accagcggaa
    29821 tcggaacaca aagatcctct acaagggccg gtacgtcaag tacccctttg agaacgggct
    29881 ataccagttg cctgacgaag atcgtttctt ctgcatcaac gagttcatca agaacctcat
    29941 cgccgtcgag aaaggcgagg tcgcgccgcc gacgaacttt gccgaatgga taaacttcac
    30001 tttcggccgg ggtatcgccg agtgttacat gatcccctac aacgagaaga tctggaacta
    30061 ccccatcaac cggatgtcgc accactgggt ggaggggcgg atcccacgcc ctccggtcga
    30121 ggacatcatt aaatcggcca tcggcatcga gaccgagggc tacgttcacc aggcggtctt
    30181 ctcctacccg gtgagaggag ggatcgaggc cctgatcagg gcaatcgcag aacccatcct
    30241 ccctgctgtc aggacaggtt tttccgtcgc ctctatccgc gaggagaacg gggggttcgc
    30301 ggtgagcgac ggccgcgaga ccgtttacgc cgatcgcctg atctcgacga tcccgctcca
    30361 gcacctgctc ccctgcctca ccgacgtccc ggcggatgtg caggcggcgt gcggcgccct
    30421 ccgctacaac tcgctctgct cggtcttcat cggtcttgcc ggggaggtgc ctgatatatc
    30481 ctggctatac gtccccgatg agaagaccgg cctcttcaac cggatatcgt tcccctcgaa
    30541 ctacagcacc gcggtcgccc cgccgggcca ctcctcgatc ctcgccgaga tcacctataa
    30601 cgagggtgat gccgtgtccc ggatgaccga tgatgaggtc atcgagcaca ccgtcgagtc
    30661 gctcatcgcc gcaggattta taccatccca tgacacggtc gtctacaccg gcatcgaacg
    30721 gcagaagttt gcctacgtcg tctacgacat cgattacctc aaaaacatcg cgatcgtccg
    30781 tgatttctgt cgggaacgga ggatcgacct tgtcgggagg ttctcacagt ttgagtacct
    30841 caatatggac ggctgtatca ggagcgccat cgactttgtg gaggggcatc catgaaggtc
    30901 aacttttttg tcgaggatat gttcttcttc aagtacatcg gctgcgccac gctggcaaag
    30961 atgctgtata gcgccctcat agaggacgat gaaccctgtc tgcaggtcgc ctggaacgct
    31021 gtcaaccgtg actttgacct cgtccactac cacacatttg gccccctcgc actggcgaac
    31081 agggcctaca gtcccggtgt caaggtgctt acagctcact cgactccccg tctcaatata
    31141 ggtaacctgg ccttctttga gagattaaac cgcctttacc cggagattta ccagggtttt
    31201 gaccacatca tcagcatctc gcccctctgc gatgaagagg ttcacgagat cgcaccggat
    31261 gttcctacaa ccatcattcc aaatggtgta gaccgcgaga agttccggcc tgatcccgag
    31321 aaaagggcgg cgttcaggga gaggtacggg atcggggagg atgagtgggt ggtgctcacg
    31381 gtcgcccagc agacaccgcg aaaggggata tacgattttc ttgcgctttc acacgaacac
    31441 atggatacca ggttcgtctg ggttggcggg ttcccttatg gcaggttctc gcaggactac
    31501 agcatcatcg aggagaagaa gagtcgctgc gggaagaacg ttatattcac gggttttgtc
    31561 gacgatatca ccgctgcata ctgcagcgcc gatgtattct tcatgccctc ctacgcagag
    31621 ggtctcccga tggtcgccct ggaggcattt gcaaccggtc tgccggtcgt cgcccggaag
    31681 atccccgagt ttactgggaa tttcaaggag atcgctgtct actttgagaa cctcgaggag
    31741 gcaggcagac tgcttgagga ccggagtctg ctcgaacagc acgcggcact ctcccgcccg
    31801 tttacagaag actacgatat ccgaaagatt gcaaaacttc atataaacct ctaccgggag
    31861 ctgacagggc agtgaggccc gtcctccgga cggtcggacg gtctttgcag gacggaaaga
    31921 gggcatcagg cttatgccac gatgccggca ataactgtac cgcaaggtgg atcgagtgat
    31981 gatctccgta gttgttccaa cctataacga agagcagaac atcgagcgct gcctgcggtc
    32041 gctcgcagac cagacggtgc cgaggaagaa ctacgagatc atagtcgttg acgggaactc
    32101 taaggacagg acgcgcgacc tggcagaacc cctggcagac caggtcttca tccagacgag
    32161 caaacgggtg ggcggggcac ggaacgacgg cgcaatggcc gcatcggggg atataatagc
    32221 cacgacggat gctgattgta tcatcccgcg ggactgggtg gagcggatcg agcggaactt
    32281 ctcagcgcat gatatagtcc agttatacgg cacggtatac ccgatcgagg gcggtctccg
    32341 gaaccggtta tcgctcctcg gtgcaaacgc cttctcacgc ctgggatact acacgaggac
    32401 gatatacttc acccttggat gcaacacggc gttcgaccgg gaggctttca tccgggccgg
    32461 gatgtaccgc tgcatcgacg ccggggacga cctggagatc gcgcagcgga tgcgtaaact
    32521 cgggagggtc cacctggatc cgggtctgaa ggtgggattc tcgatgcggc gctaccagca
    32581 gttcgggact atcaggtcgc tgtgggagtg gttctacatc gttcgttccg gcggcgaggc
    32641 aggcgatgcc acatacacga agcgggagta caggtgaggc ggcctgatca tgaatggttc
    32701 tgcat
//
LOCUS       DAOSZX010000006        30305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1247_length_30305_cov_20.808760, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000006 DAOSZX010000000
VERSION     DAOSZX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 30305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30305
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1247_length_30305_cov_20.808760"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1597
                     /locus_tag="PK196_01130"
     CDS             230..1597
                     /locus_tag="PK196_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40061.1"
                     /translation="MTADVLLINPPDTKSKYKKYFNIRIPPLGILYIAAVLEENGVGV
                     EVIDCAAGEIDYSGIEKRVEEAEPFLVGITSTTPLIREALRSAEAVRRVSDAYIVLGG
                     PHATFMHNEILRENASIDFIVKGEGEYTVLDLYRTLRTGGGLAAVDGIVYRKGERVVE
                     NPDRPPVEDLDTLPYPARHLVPRENYRVFDDNLPVATMICSRGCPMQCSFCASSALHG
                     KHVRRRSTGDVVDEIRQIQDNLGISAIGFMDDTFTLYPRWVEEFCREVIEQDLGIEWG
                     CTARVDRIDRSLMEVMKKAGCHTLFFGVESGDQGILDRVKKGTRIDQIKQVFATAHDL
                     GMRTIASAAIGLPGETRETANRTIKFVKSLKPSFALFSVATPYPGTEFFRKVHEDGGL
                     HTDWSRYDLLSPVVETSSLTLEEIKKLQKKAFREFYLRPSYLIQGLRREGFSFLKIAK
                     SALSA"
     gene            complement(1689..1760)
                     /locus_tag="PK196_01135"
     tRNA            complement(1689..1760)
                     /locus_tag="PK196_01135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1726..1728),aa:Thr,seq:cgt)
     gene            complement(1836..2132)
                     /locus_tag="PK196_01140"
     CDS             complement(1836..2132)
                     /locus_tag="PK196_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HON40062.1"
                     /translation="MANHEISVNIPSNLDPVYSNRIQIAYKEDEFTFLFLHEIPAANQ
                     ARAKAIVSISPRHAKNLVEVLSKSIADYEQKFGKITAPSPAQQQEGNVTIRGYS"
     gene            2332..2994
                     /locus_tag="PK196_01145"
     CDS             2332..2994
                     /locus_tag="PK196_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HON40063.1"
                     /translation="MNEYIPVSGVIACHICPRRYYFERSDELPESPRYTVCKQVSMHL
                     WELLDPEEIWSEVLCILPEVPDEARELFDGCIAACSGTSWQRPVQTDLVVESDQFGIR
                     GRVDKVFEDGTFAIVRSSTAPKAGVYETDRIRIACYIACLRETLGRPVAGGYVEYIAN
                     GLSRYVEPQPRDRRAMLRAIRAAKRVVAGEIPPRPIRAPCEDCPHFERCTAGPRRLSD
                     LF"
     gene            complement(3033..3965)
                     /locus_tag="PK196_01150"
     CDS             complement(3033..3965)
                     /locus_tag="PK196_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HON40064.1"
                     /translation="MRPIRLRDFIEDRDGCLYAVSAYDNNERVGCILRYVPDPEGERR
                     DPAGRRYRKYDFSEAFAWVREHKPDYLDTVHRVPREDVVRVYKPEEEIGNVAARDHRV
                     ARLLSHFDLPAGSFGCTGSLLCGLENAASDIDLVVYGEAWFTAQRHLRRLVETGMIPP
                     MSTAMWRKVYEKRAPEIPFDAFVLHEQRKWNRGEFEGTYFDLLYTRSYADLDALPTGA
                     GKVIGRATIEATVTDASLSFDSPAVYAVDQDEISRVLSFTHTYAGQALAGEVIEARGV
                     VEEHDDELWLIVGTTREARGEYIISKTLLENAGY"
     gene            complement(3943..4545)
                     /locus_tag="PK196_01155"
     CDS             complement(3943..4545)
                     /locus_tag="PK196_01155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HON40065.1"
                     /translation="MDPIDMAVLVLRRDGLVVYPTETVYGLGADALSEDAVRRVYEAK
                     NRPVGKPISVAVSDMEMLETIAEVDDLARAFIKQFLPGPVTVILPARSCLPEILTGGT
                     GLIGIRWPNNEIAIELISRLDAPITSTSANISDDIAPTRPDEVSAPHDYLIDGGELPG
                     TPSTVVNLSARRILRKGAEWEAVEAFLETLRWKHAADTTP"
     gene            complement(4545..5225)
                     /locus_tag="PK196_01160"
     CDS             complement(4545..5225)
                     /locus_tag="PK196_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HON40066.1"
                     /translation="MSSDTDNMYALSIITENRTGVLRDVATVMADYMANIQMIQQSII
                     ASGPNAGRASIYLEFNECGNHGDMVADLARVPSVIEVTTYPPFSRIFGSRVIIIGGGA
                     QVAQVAMGAVNEADRHNIRGERISVDTIPLVGEQNLALAVDAVARLPRASILVLAGSL
                     MGGEVSRAVDRVRAAGIPVIALKMAGSVPQHADLVVTDPIQAGVFAVMHISKTAVFDI
                     NRVRGREF"
     gene            5670..>6348
                     /locus_tag="PK196_01165"
     CDS             5670..>6348
                     /locus_tag="PK196_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40067.1"
                     /translation="MDGKHRAAYTVILLFGVISLFGDIIYEGARSVAGPYLYLLGAGA
                     LAVGVVSGLGEFLGYALRLASGYLADATRHYWAFTFIGYGMLVAVPLLALAGSWEIAA
                     LLLILERVGKGIRVPARDAILSNATAAVGRGWGFAVHEALDQIGAIVGPLLFAAIFLL
                     DGDYRGGFALLAVPFVLMILALLFARRRVPEPTSLEGSGPAPGEALSLPRTLLPYGVF
                     TALTMAGF"
     assembly_gap    6349..6448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6449..7000
                     /locus_tag="PK196_01170"
     CDS             <6449..7000
                     /locus_tag="PK196_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40068.1"
                     /translation="PRTLLPYGVFTALTMAGFATFPVIAYHFAVTGTVTEAEIPLFYA
                     VAMGVDAVAALAIGRAYDRFGLSVLLSVPLLGICIPLLAFGTDHALAFAAVVLWGAAM
                     GAQETVLRAALADYTRISKRGTAYGVFNTIYGAAWFAGSAVIGWAYTFSIPSVIGFLV
                     LMQVLALGAFAWMRRGFSSAAAS"
     gene            complement(7133..8347)
                     /locus_tag="PK196_01175"
     CDS             complement(7133..8347)
                     /locus_tag="PK196_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HON40069.1"
                     /translation="MKSVELAPGVHWVGAIDWNLREYHGYTLPGTTYNAYLVRGEKTA
                     LIDGAYHGFEKEVLGRIRSVCDPAEIDCIVVNHIEMDHSGTLPEFMRRMPGTPIYCTA
                     RAKAGLARHYDTTGWEIHVVRSGDTLDLGGKTLTFLEAPMLHWPDSMFTYLGEDAILF
                     PNDAFGQHVASAARFDDELGIDAAMAHAKKFFANLIIPLAPKVLKKLDEVGNLGIDIR
                     MIAPSHGIIWRSHAGEILKAYTDWSHGVSKDKVTIVYDTMHGSTTMLAKAIAEGVMAE
                     GVDLRVCLLRDGRYEGTHRSDIVTEILDSKAVLVGSPTLQDEVLPTVAGFLSYLRGLQ
                     PGRLTAKKIGCGFGSHGGMGGAVARIEEALKEAGIEVIDGGFHVNYRPDADELTRAYE
                     LGRRVAREIRAR"
     gene            8438..9229
                     /locus_tag="PK196_01180"
     CDS             8438..9229
                     /locus_tag="PK196_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40070.1"
                     /translation="MEPGMGVRGRPGRPKPKQEIDLYSTPEGAQVIDNPVRRAILAAL
                     HERERTFEEVVLLVGRAKSTVSVHLQELAAAGMVGSRADPDDSRRKVFYLTGDLVASL
                     SPEERLAGDLAAYAGAYRPGMGDPFEFYRLAFRTIRIALMHEGIVLDPLLTRAGKGVG
                     EELYPALADPDIEHFCTKIARFWEEHHLGRVEVERQDPLTLLVYDCFECADLPVTGRP
                     ACAFDSGILSALFSHHYGRSAAAIETRCYSMGFDHCRFEITLEGS"
     gene            9527..10354
                     /gene="modA"
                     /locus_tag="PK196_01185"
     CDS             9527..10354
                     /gene="modA"
                     /locus_tag="PK196_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HON40071.1"
                     /translation="MKQSKTAILVALSLVVIASLMAAGCTGTGETPEETTLTVFTAAS
                     LTGAFTDIARAYEAENPGIKVDLVFDGSQTLRTQIEQGANADIFVSASTKHMQALQNS
                     GFMDNDTVSDFVGNSMALIVPIDNPANITGLADLAKPGVKIVIGTKDVPFGDYTRQVL
                     DKLAADPAYGEAYREAVMKNVISEETAVSNVVPKLALGEADAAFVYKSDVSKEDLTRV
                     ARIEIPEDYNVQATYPLGILANSPHKAEAESFIEFVRGPAGSAILQDYGFDPLPAGS"
     gene            10323..11165
                     /locus_tag="PK196_01190"
     CDS             10323..11165
                     /locus_tag="PK196_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40072.1"
                     /translation="MDSTPSRQGREVLRHAGRARLSRGFLTLFVAALLVLFLLFVTLP
                     VVSLFLRISPEAFFRSLAEPVVIDALSLSLTTATASTAIVVLLGTPLALVNARHDYPG
                     RALVDTLTDLPIVLPPAVAGIALLMAFGRRGVVGQYLNEFGISIAFTTAAVILAQVFV
                     ASPFYIRQARASFEAVDRHYEDAARTLGASPATVALRVTIPLAWGGLVSGAILSFARA
                     LGEFGATIMFAGNYQGRTRTMPLAIYTTMQGDMDAAISLAIILVVISFVVIAAVKVIT
                     RRNY"
     gene            11165..11875
                     /locus_tag="PK196_01195"
     CDS             11165..11875
                     /locus_tag="PK196_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40073.1"
                     /translation="MLEFRAVRKLRDFVLDLALSVLPGETLVLIGENGAGKSTVLNLI
                     SGILTPDEGEITLGDRVLFSSEERINLHPENRNIGHLFQSYALFPHMTVAENVAFGLR
                     CRKLPEPEIKRAVAEQLETINLSELADVNVGRLSGGQRQRVALARALVLDPDLLLLDE
                     PLAAVDMRSQAAMRRELRERIRKTGIPCIVVTHSLRDAVELGDRLGLMEDGHLVAGGR
                     PEEVLRMRENRFIASFVG"
     gene            complement(11853..12755)
                     /locus_tag="PK196_01200"
     CDS             complement(11853..12755)
                     /locus_tag="PK196_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HON40074.1"
                     /translation="MTAILLRPDQPLDLDRTLSCGQAFRWEEADGWWNGVVGEEAIRI
                     RQDGNRLLFSGADAQFIHDYLRLDQDLPSILASIDRDPVIGAAIRECMGLRLVRQPPW
                     ECLVSYICATNTNIPAVKRRIALMAERYGRPIEGPFGRAYAFPEPDALAELSCTDLRE
                     CKVGYRADAVREAALYAAENPDWAERIAALPFEEAREALMHFRGVGPKAADCVLLFAF
                     GFFEAFPVDVWIHRIMTEAYLPDLAGRGCTPAVCERIRKFARGYFGDYAGYAQEYLYC
                     VRDREMQKIVKPPFLDPATRQNSQ"
     gene            complement(12780..13691)
                     /gene="nifB"
                     /locus_tag="PK196_01205"
     CDS             complement(12780..13691)
                     /gene="nifB"
                     /locus_tag="PK196_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HON40075.1"
                     /translation="MADEYRTATVQGREVPYDPEQLRKISEHPCYSDKACHAFGRCHL
                     PVAPRCNIQCNYCVRDYDCVNESRPGVTSRVLTPEEGLDLVRKVIRDYTYVKVIGIAG
                     PGEPLANPETFETLRLVHEEFPHLIMCISTNGLLLPESIDDLERYDVGNVTVTLNAVD
                     PAIGEKIYSWVEYEGKRHHGREAAELLLSQQLRGIEMAVAKKMFVKINTVYIPGINDD
                     HIPEIAKKAGEMGAFTFNVIPLIPQYKFADITPPTPAEKRDMQDRCAPYIKQMRHCAR
                     CRADAIGKLGQDVQSCVYQQMKDEKAE"
     gene            complement(13928..15823)
                     /locus_tag="PK196_01210"
     CDS             complement(13928..15823)
                     /locus_tag="PK196_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HON40076.1"
                     /translation="MYVKPDWWRGIFNNLYLKTDGDVVEDPRITASEIDRIIRILRLQ
                     PDDKILDLCCGQGRHTLELARRGYSAEGLDRSHYLIQRARSTAKKEGLTVRFREGDAR
                     RLPYRNDTYDVALVLGNSFGYFDSVEEDMQILNELRRVLKPWGRLLLDVADGSYLKAH
                     FQPRSWEWIDTGMFVCRERSLSLDEQRLISREVITDVEKGVVADQFYAERLYTPDALQ
                     RLLVRAGFTEIAFHEIATESERNQDLGMMERRYIVTAQIRKEWSTTRARGQGRTKHVA
                     VLLGDPDKPDPLKPSCVFDDDDIYTIDRMKAALRELPGYRFTYLCKHETLIRDILRLK
                     GRVDYVLNLCDEGFNNDPGRELHIPALLEMLDIPYTGAGPQSLAFCYDKSLVRGIAKE
                     MGIPVPEACFIAPGDRTYEVGVNFPVIVKPNTGDSSYGITAASIAHTIEELSNVINSL
                     RATLGYDRSLLVEEFLTGKDISVGIIGNPPGPCTVLPIIEEDYSALPPDLPRICGYEA
                     KWLPDSPYWKITSKPANLPEGTEKLIVRCCISLFERLECRDYCRFDWRLDAQGNPKLL
                     EVNPNPGWCWDGHLAKMAKYAGLSYSGMLGRILKAAEERFGMEPENGAREEERAEQLP
                     LDAGEVA"
     gene            16220..16396
                     /locus_tag="PK196_01215"
     CDS             16220..16396
                     /locus_tag="PK196_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40077.1"
                     /translation="MNERSPRKGPRSFQDRPREFHKAVCSDCGKECEVPFKPTEGRPV
                     YCRDCLPKHRKPRF"
     gene            complement(16416..17183)
                     /locus_tag="PK196_01220"
     CDS             complement(16416..17183)
                     /locus_tag="PK196_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40078.1"
                     /translation="MIECIQRVGSVWQRNLEAFLRTYRVNFIPPFIEPLLYLLALGFG
                     LGTYIEAVDGVPYPVFIAPALVSISVMNSAFYECTYSSFVRMYYQKTFDAIIATPVSI
                     DEVIAGEMIWGATRGVIYAGLMLPVLILFGVAAMPSSLLLIPFAFIAGLLFAGIAMCF
                     TAITPSIETLNYPSFLFITPMFLFSGTFFPLELLPDAVRCAALAALPLTHVVEINRAI
                     TLEAVSAANLLNLAWIAVATAIFCSLAIRLMRRRLVV"
     gene            complement(17191..18102)
                     /locus_tag="PK196_01225"
     CDS             complement(17191..18102)
                     /locus_tag="PK196_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40079.1"
                     /translation="MGDIIAAHDLEKRFGDVAAVDRVSFRVREGEVFGFLGPNGAGKT
                     TTMKMIQCISPKSGGSLEVFGMDVDTHPREIKSRIGVVPQENNLDPDFTAYRNLLVYA
                     RYFGIPKSVAEERAERLLGFMRLEEKRDVLIEKLSGGMKRRLIVARALVNEPELLILD
                     EPTIGLDPQARHLIWETLRGLRAEGNTLVLTTHYLDEAERLCDRLVIMDHGRILIEGA
                     PAEIVHEYAGNEVIEIERTEKTAALLDKLGVAYDVAGDMLQVFTDRADEIVGELLDLC
                     RHEAAITVRPATLEDVFLRLTGRKLRE"
     gene            18365..18721
                     /locus_tag="PK196_01230"
     CDS             18365..18721
                     /locus_tag="PK196_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HON40080.1"
                     /translation="MSKCHGRGVFARRDLLAGEVIEVCPVIVLGGADEQELLDKTHLF
                     DYYFEWGGLAAVALGYGSLYNHSSHANADHVCDVHRGEIRIYAHRHIRQGEEITINYG
                     GRPDCPDPVWFDAVEG"
     gene            19294..>19703
                     /locus_tag="PK196_01235"
     CDS             19294..>19703
                     /locus_tag="PK196_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HON40081.1"
                     /translation="MVWGEENVRRVCARLRSAKTRAELAAILYQEVSRYSLSDLQAVR
                     GRVERDLRFVPPAYRQRLYPRLVEQVFDTHHALISTIRQGRLRVGGEPLPPEYEEFCD
                     MVERTCLAGEEDEQHLELLYFLLAAFNIFVLDLPA"
     assembly_gap    19704..19803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19804..20000
                     /locus_tag="PK196_01240"
     CDS             <19804..20000
                     /locus_tag="PK196_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HON40082.1"
                     /translation="LLYFLLAAFNIFVLDLPAHPVGTPFPGGLKVEARNGEYLCPVRE
                     KANDVENALCPYCPAKQSEI"
     gene            complement(20188..20718)
                     /locus_tag="PK196_01245"
     CDS             complement(20188..20718)
                     /locus_tag="PK196_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON40083.1"
                     /translation="MSSIGGAVNLAVTVIRSRHSVRKYKDTPIDDKIVKDALDCAHLA
                     PTARNEQPWLFGAIRDRDTLKAIAGLADNGRFIANAPICFAVFGKKDAKYYLEDCCAA
                     TMQLILALQGWGVGSCWVAGAKKDYAEDVRKLLNVPEDYTLVSLVPAGYPEEISIAKK
                     KPLDEVAFFDRYEEEK"
     gene            complement(20798..21997)
                     /locus_tag="PK196_01250"
     CDS             complement(20798..21997)
                     /locus_tag="PK196_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HON40084.1"
                     /translation="MAEIRLVGTAHVSQKSVEEVRSAIEEFQPDIVGVELDRGRFISL
                     TEETAEPSVTEILKSGNFGQILLQWILTYIQQRIGAETGVKPGSEMLAAIEEARAHQK
                     PVAFIDRDIRITLSRFWGLMGIREKIRLIWALLYSLVSVDTQEIEIDELTDQDVVSVA
                     MEEFRKFSPRGAQALIDERDAYLAHQILMLARRYDRVLAVIGAGHIKGVQRYLDAPET
                     LPLLSDLTAEVKKGLPWAKILGAGVTILFLLLIAAIAFSGVGLDVLLTAIIYWILING
                     LLSAAFTFIAGGHPLSAATAFAVSWMTSLNPLLAAGWFAAIVEAKIRKPASGEIRQIM
                     EAETFSEMRRIPLFRVVLVAALANVGSSIGTFAYFIFIFPVLGIDPAMVIGDGFSNML
                     QAIAGLL"
     gene            complement(21990..22532)
                     /locus_tag="PK196_01255"
     CDS             complement(21990..22532)
                     /locus_tag="PK196_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HON40085.1"
                     /translation="MLGVDEIAERLGSIGFCCRECGRCCRRVGEDSNLVLVGAAELRE
                     IMAATGMKREEIVEPYPEFIRAGNGGEYTLAWCLRRTLDACIFLKDGRCSIYEHRPWI
                     CRTYPFMLVDDDLMVSECPGLGASISPSAARDAAADLCRRQAAEAEEEAEIREIFLEA
                     EIPPGKRVVIDSEGMKVLDG"
     gene            complement(22520..23074)
                     /locus_tag="PK196_01260"
     CDS             complement(22520..23074)
                     /locus_tag="PK196_01260"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HON40086.1"
                     /translation="MLPLYVVNNHGQFNHLILRTLRDMDIEAGMISNMTPPDEVANGC
                     RGIILGGGPTLERAGLSGDYLDLNIPVLGICLGLHIIATARGGAIRRGASGGFGGVEV
                     EVLEQDPLLQGYPERMQVWASHADEVSVVPEGFLRVARSAICDVEAMVSPEERLYGIQ
                     WHPEVSHTFNGRLLFENFDRICSE"
     gene            complement(23114..24130)
                     /locus_tag="PK196_01265"
     CDS             complement(23114..24130)
                     /locus_tag="PK196_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HON40087.1"
                     /translation="MSHPFLSEDPGFRPRRPMMVVVLGNTMLSTVPGISGAGPTPEKT
                     LLTPILDAELITTGAITSMPARPNTPTGCPTPASITRSMMELTGLSPIFINAGLAHTP
                     TVPCLDVYGRPGGDPRIEDAVPDAPLLFERGEAIGRIISHYSDLLMLGECVPGGTTTA
                     LCVLRALGYDASVSSAFPRNPIDLKEGVCREVLARIRENGIEEPMGILRAAGDPMMPV
                     AAGIVNTYSGDLIFAGGTQMLAVAAVLKALGRRVPRLATTVYVRDDPSAGFSRSVAEV
                     GTTAYYVDPNFENIGHPGLARYCIGEVKEGAGAGGALVLAYLMGHRPEEISRKVFDFV
                     MKYA"
     gene            24271..24714
                     /locus_tag="PK196_01270"
     CDS             24271..24714
                     /locus_tag="PK196_01270"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HON40088.1"
                     /translation="MFEIEQRLEGKGILRRDIVAAAMELYIPHGIDEDEAARRLDAKI
                     GQALDDPNVSSLLLGALLLEDELYTKRENSEIADDPVFLLSDEIIGMAIAEVIGGTYA
                     RFEFTRYDQKKPGVLKELGPFLDDAVAGLIAGCTSRLYSESMGSV"
     gene            24711..25421
                     /gene="cobS"
                     /locus_tag="PK196_01275"
     CDS             24711..25421
                     /gene="cobS"
                     /locus_tag="PK196_01275"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HON40089.1"
                     /translation="MRSVLALLQFCTTLPLGRVVDFEHFARRSYLYPIAGYIIGGVAA
                     IVTFWIASPSVGAAVAIAAVLLLSGCNHFDGLLDLGDGLMAHGGREKRITAMTDRATG
                     AGAVAAGILVTLLAFSGLQAAANIPLTILTAEVCAKTAMSWLTTLGRPFREGIHSYLH
                     SFAKPWFLVPAFLLTLPLFLLPLPRLALVLALAATAAITALMLVLSRRVFGGVNGDIV
                     GASNEIVRAGVILLLALA"
     gene            25464..26342
                     /locus_tag="PK196_01280"
     CDS             25464..26342
                     /locus_tag="PK196_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40090.1"
                     /translation="MQEQSLYSRGGIITERDAEYATVRVRIPAGVLSAAQVRQLGKIS
                     EKYGDGTLHLTMRQTVEIPHVKPENFEKVAKALEKNGTPLGAEQNEVVNIMACPGTER
                     CKYANCETIDLARKIDERVFGKVLSIRLRIAISGCPYMCNSPLLNDIGIIGRIRPLRI
                     PGLCTGCGTCVEYCKQHAIALKNGVSVLDESKCVQCGVCIHSCPYHLLKSEYDHYQIT
                     VGGRRGASPAAGRELVTVETAEEVVEVVDRIVYWVYRSAWSGRPLADQMDEIGYAKFR
                     EEIQKEFGPKPSEEKR"
     gene            complement(26406..26708)
                     /locus_tag="PK196_01285"
     CDS             complement(26406..26708)
                     /locus_tag="PK196_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40091.1"
                     /translation="MKLLEFHRDEAKDRVTVTCAGREVSVYSHCAYCRYCAGVRVGKR
                     TMATPQRQALSGVRHGSNPDENLLNAAMMFNSLVRDGTGIECEDEAGEGFASMYYR"
     gene            26886..28163
                     /gene="tuf"
                     /locus_tag="PK196_01290"
     CDS             26886..28163
                     /gene="tuf"
                     /locus_tag="PK196_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HON40092.1"
                     /translation="MAVEKPHLNLAVIGHIDHGKSTTVGRLLFETGAVPPHIIETYRK
                     EAEAKGKGSFEFAWVMDSLKEERERGITIDIAHRRFDTEKYYFTVVDCPGHRDFVKNM
                     ITGASQADAALLVVAAPDGVMEQTKEHVFLARTLGINQLIVGINKMDAVKYDEKRFNE
                     VKAELSQLLKIVGYKPDNVIFIPMSAFKGDNISKHSENTPWYKGPTLLEALNMLSEPE
                     KPTNLPLRLPIQDVYSISGIGTVPVGRVETGVMKKGMKVSFMPANKEGEVKSIEMHHE
                     EIPEALPGDNVGFNVRGIGKGDIRRGDVCGPADAPPTVAEEFVAQVVVLHHPSALTVG
                     YTPVFHCHTAQIACTFVELQKKLDPRTGQVKETNPTFLKTGDAAIVKIRPTRPMVIEK
                     VKEIPQLGRFAVRDMGSTIAAGMCIDITPKQMR"
     gene            28194..28502
                     /gene="rpsJ"
                     /locus_tag="PK196_01295"
     CDS             28194..28502
                     /gene="rpsJ"
                     /locus_tag="PK196_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HON40093.1"
                     /translation="MQKARIRLSGTDFEKIEMVCDRIKEIAERTGVNLAGPIPLPTKK
                     LVVPARKSPDGEGTETWDRWQMRVHKRLIDIDADERALRQLMRIQVPKDIGIEIVLES
                     "
     gene            28515..30203
                     /locus_tag="PK196_01300"
     CDS             28515..30203
                     /locus_tag="PK196_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HON40094.1"
                     /translation="MKAAAFTARVRENLSLEVVFLLIFLAAVALRFYALDLKLFHHDE
                     AVHAWFAYNLLTDGTYIYDPMYHGPFLYYVTAGVFSLLGDSDLTGRLLPALFGSLLLL
                     LLYPIYRLGYLDKKQTLVAALFLAVSPNMVYFSRFLRNDIFIAFFTMLLLVALLYYFE
                     RGQTRYALLAGTAIGLGMSAKENMPIVILIFGLYLIYLIWTGKVRLPAHWVRDLVLGV
                     VVAVGIATVFYSSFGAHPEMALDWWLRAIEHWTAMHEMQRIGGPPYFYILLFILYEAP
                     ILILAIVGVLQFFDVAGIISSWRERRQDMEKPGETGVTDVDNVDKEETPDPLPPPPEV
                     AARGLRGRLRDIISGSGEIRQIDRQKEFARFAIFWMLLSLAAYAYIGEKVPWLILHQL
                     LPMIFVAVYQMTTKKAVIAVVASILLVAMTFHVAFTPADINEPIVQVQNSEDLRWLFA
                     KIDAADRVAIATDSYWPLPWYYRGDRSSKIAYYGQKVSEETIYSGNFDLVVTHDADTY
                     PALDGYVKETCRLNYWFSYGENKDRLLAYYFFRDGKVGSRILEVFSRASAGTAT"
BASE COUNT         5892 a         8977 c         8764 g         6472 t
ORIGIN      
        1 ggtctcttct ccgatagaga gttctcggcc gggagagggg atagttcttt ctctaccgtg
       61 atcgagccgc tcttccggga tatacgggaa ggttatcatg caaagcagcg ccgtttcctg
      121 tggatgctgg agggtctttt ggtgcctgcg gtatactgac caccgcaatc cggggatctt
      181 cccgcctgtc agtcagttct ataatgaaag atataccatt gtaaaaagaa tgaccgcgga
      241 tgtactcctg atcaacccgc cggacacgaa gtccaagtac aagaaatact tcaatatcag
      301 gatacccccc ctgggtatcc tctacatcgc tgcagtactc gaagagaacg gcgtgggcgt
      361 cgaggttatc gactgtgccg cgggagagat cgattattcc gggatcgaga agcgcgtgga
      421 agaggccgaa ccgttccttg tcggcatcac ctccaccaca cccctgatcc gcgaggcgct
      481 gcgttcggct gaagcggtca gacgggtatc cgatgcatac atcgtgcttg gcgggccgca
      541 cgccacgttc atgcacaacg agatcctgcg ggagaacgcc tccatcgatt tcatcgtgaa
      601 gggtgagggg gaatacaccg ttctggacct ctaccgaacg ctcagaacgg gtggcggcct
      661 tgcagcggtg gatggcatag tctaccgcaa gggagagcgt gtcgtcgaga accctgacag
      721 acctccggta gaggacctgg acacattgcc atacccggcc aggcatcttg tcccccgcga
      781 gaactaccgg gtctttgatg acaacttgcc ggtcgcaaca atgatctgca gcagggggtg
      841 tccgatgcag tgttcgttct gtgcatcatc cgccctccac gggaaacacg tcaggaggcg
      901 ctctaccggg gacgtggtgg atgagatccg gcagattcag gacaatctcg ggatctcggc
      961 catcggattc atggacgaca ccttcacttt atacccccgc tgggttgaag agttctgcag
     1021 agaggtcata gaacaggatc ttggcatcga gtggggctgt accgcgagag tcgacaggat
     1081 cgatagaagc cttatggagg ttatgaaaaa agcaggatgc cacacgctct tttttggtgt
     1141 ggaatccgga gaccagggga tactcgatcg tgtcaagaag ggtacgcgta tcgatcagat
     1201 caaacaggtg tttgctaccg cacacgacct cggcatgagg acgatagcct cggcggcgat
     1261 cggtctccct ggagagacca gggagacggc aaatcggacc ataaagtttg taaaaagcct
     1321 caaaccgagt tttgccctct tctcggttgc aacaccgtac cccgggacgg agtttttcag
     1381 gaaggttcat gaggacggcg gtctgcatac ggattggtcc aggtatgacc tgctctcacc
     1441 ggtcgtcgag acctcgtccc tcaccctgga agagatcaag aaactccaga agaaagcgtt
     1501 ccgtgagttc tacctcagac cgtcgtacct catccagggc ctgaggcggg agggtttctc
     1561 tttcttaaag attgcaaaat cggctctctc cgcctgaggc ggtttttgtc ggggagggcc
     1621 tcactcatcc ctccggggga gcgtcagaag agagttgcgg gattataggc ctttgaattg
     1681 ataaagtaag ccgctggagg gatttgaacc cccgacctgc tgattacgaa tcagccgcta
     1741 taccactaag ccacagcggc aactgcccta aagcattcca cctctaaaat taaaaaggta
     1801 tcacctcccg gaaaccagga ccgcaccggc caccatcagg aatagccacg gatggtgacg
     1861 tttccctcct gctgctgggc aggcgacggc gcggtgatct tgccgaactt ctgctcgtaa
     1921 tcggcgatgc tctttgagag cacctcgacg agattcttcg cgtgtcgagg actgatcgag
     1981 acgattgcct tcgcccgcgc ctggttcgcg gccgggatct cgtgcaggaa gaggaaggtg
     2041 aactcatcct ccttgtaggc gatctggatc cggttcgagt agaccggatc gaggtttgac
     2101 gggatgttga cagatatctc atggttagcc atactcatct tttatgaccc gcactggcat
     2161 aaggtttggt ctctcaatgg aagaacgacg ccattttgac cggcgccggc gtggtcttcc
     2221 cgccataatc ccggccacag gcaaccctgc ccgttgagag cgagggcacg aagccccttg
     2281 ttttatagcg ttgccttgcg caacctttcc tgaaagttcc cgcttgacag aatgaacgag
     2341 tatatccctg tatccggcgt catcgcctgc cacatctgtc cacggcgcta ctactttgag
     2401 cgctcggatg aacttcccga gtcgccccgc tacaccgtct gcaaacaggt ctccatgcac
     2461 ctatgggaac tcctcgaccc ggaggagatc tggagcgagg ttctctgcat cctccccgaa
     2521 gttcctgatg aggccagaga actatttgac ggttgtatcg ccgcctgcag cgggacctcc
     2581 tggcagcgtc ccgtccagac cgaccttgtc gtcgagtccg accagttcgg cattcgcggc
     2641 agggttgaca aggtctttga ggatggaacg tttgccatcg tccggtcgag caccgccccg
     2701 aaagccgggg tctatgagac cgacaggatc cggatcgcct gctacatcgc ctgcctccgg
     2761 gagacgctcg ggcgtcccgt tgccggggga tacgtcgagt atatcgcgaa cggtctctcc
     2821 cgctacgtcg aaccccagcc ccgggatcgg cgggcgatgc tcagagcaat ccgggctgca
     2881 aaaagggtgg tcgcgggcga gattccgccc cgccccatcc gggcgccatg cgaggactgc
     2941 cctcactttg aacgctgcac tgcaggaccg cggcggctct cagacctctt ctgagagaat
     3001 ggggcggcag gagagaacgc ttctgttacc cttcagtatc cagcgttctc gagcagggtc
     3061 ttcgagatga tgtactcgcc cctcgcctcc cgggtcgtgc cgacgatcag ccacagttca
     3121 tcgtcgtgct cctccacgac acccctcgcc tcgatgacct ctcccgccag cgcctgcccg
     3181 gcgtaggtgt gggtgaagga aagcacccgg ctgatctcgt cctggtccac ggcgtagacc
     3241 gccgggctgt cgaaagagag ggaggcgtcg gtaaccgtcg cctcgatcgt cgcccggcca
     3301 atcaccttcc ccgccccggt cgggagggcg tccaggtccg cgtatgagcg tgtgtagaga
     3361 aggtcaaagt atgtcccctc gaactcgccc ctgttccact tccgctgctc gtgcaggacg
     3421 aaggcatcaa acggtatctc cggcgccctc ttctcgtaga ccttccgcca catagcggtg
     3481 ctcatcggcg ggatcatgcc ggtctccacc aggcgacgga ggtggcgctg ggctgtgaac
     3541 caggcctcgc cgtagaccac gagatctatg tccgatgcag cgttctcgag gccgcagagt
     3601 agcgatcccg tgcacccaaa cgatcccgcc ggcaggtcaa aatgggagag aagccgtgcc
     3661 acacggtggt cgcgggcggc cacgttgccg atctcctcct ccggtttgta taccctgacc
     3721 acatcctcgc ggggcacccg gtggacggtg tccagataat ccggtttatg ttccctgacc
     3781 caggcaaacg cctcagaaaa atcgtatttg cggtagcgcc gccctgcagg gtctcttcgc
     3841 tccccttctg ggtcgggaac gtagcggagg atgcacccga cccgctcgtt gttgtcgtat
     3901 gccgagactg cgtagaggca tccatcgcgg tcctcgataa agtcacggag tcgtatcggc
     3961 cgcatgtttc caccggagtg tctccaggaa cgcctccact gcctcccatt ccgctccctt
     4021 cctcagaatg cgccgtgcgg agaggttgac cacggtgctt ggcgttccgg gaagttcccc
     4081 cccgtcgatc aggtaatcgt ggggggcgga gacctcatcg gggcgggtcg gcgcgatgtc
     4141 gtccgagatg ttcgcgctgg tagacgtgat cggggcatcg agtctgctga tgagctctat
     4201 ggcgatctcg ttgttgggcc accggatccc gataagaccc gtccctcctg tgaggatctc
     4261 cggaagacac gatctcgccg ggaggatcac cgtcaccggg cccggcagga actgcttgat
     4321 gaacgccctt gcgagatcgt ccacctccgc gatggtttca agcatctcca tatccgagac
     4381 cgcgaccgag atcggtttgc cgaccggtct gttcttggcc tcatacaccc tcctcaccgc
     4441 atcctctgag agggcgtccg cacccaggcc atagaccgtc tcggttgggt agactacgag
     4501 gccgtcacgc cgcagcacca ggacagccat atcgatcgga tccatcaaaa ctcacgcccc
     4561 cgcacccggt tgatatcaaa gaccgctgtt ttgctgatgt gcatcacggc aaaaacccct
     4621 gcctgaatgg ggtcggtcac gaccaggtcg gcatgctgcg ggacactccc ggccatctta
     4681 agggcgatca ccgggatgcc tgccgccctg acccggtcca cagcacgcga gacctcgccg
     4741 cccatcagcg atccggcgag caccagtatc gacgccctgg ggagtcttgc tacagcgtcg
     4801 acggcgagcg ccaggttttg ttcgccgacg agggggatcg tgtccaccga tatcctctcg
     4861 ccgcggatgt tgtgccgatc cgcctcgttc actgcgccca tcgccacctg cgccacctgc
     4921 gccccgccgc cgatgatgat cacccttgac ccgaatatcc gggagaacgg ggggtaggtg
     4981 gtgacttcga tgacagaagg cacacgcgca aggtcagcga ccatgtcgcc gtggttcccg
     5041 cactcgttga actcgaggta gatggaagcc ctcccggcgt tcgggccgga ggctatgatc
     5101 gactgctgga tcatctggat gttggccata taatccgcca taacggtggc cacgtcgcgc
     5161 agcacacccg tcctgttctc tgtgatgatg ctcagggcgt acatattgtc ggtatcgctg
     5221 ctcatgaccc cccccggcag gagtttgaga cctcctccaa tctctccaga aacagggaat
     5281 tttttgtcat attcgcgctg gtggatataa ttgttcctca tgagggttat aggttttcgg
     5341 tgagcgaggg tcgctggaaa gaggccatca aacaatagtc gtactggttt gataaacctc
     5401 tccagcgacg gattccgggg ggtggccggg tggtgggggt gtttccaccc taaatggaag
     5461 agagtttccg ggtgacatgc caagacatgg gggggaaaag ttcctttccc ccctcccccg
     5521 cggtggcgat tctccatcga tacagtgtcc ggcgtagcat cactaccgtc atatttgaag
     5581 gcttctggaa agggtctcct gaacagaccc cgggcaggtg gtgcccatca ataaggctta
     5641 tggcctatca acaagagaat tgtcttctcg tggatggcaa acaccgcgca gcctatacag
     5701 tgatacttct ctttggtgtt atcagcctct ttggcgatat aatctatgaa ggggcgcgga
     5761 gcgttgccgg gccgtacctc tacctccttg gcgccggcgc ccttgccgtc ggggttgtct
     5821 ccggactggg tgagttcctc ggctacgccc tccgtctggc gtcgggttac ctggccgatg
     5881 ccacccggca ttactgggcg ttcaccttca taggctacgg gatgctcgtt gccgtcccgc
     5941 tcctcgcgct tgccggatcc tgggagattg ctgccctcct cctcatcctt gagcgggtgg
     6001 ggaaaggcat ccgggtgcct gcacgcgatg ccatactctc taacgccacc gcggcggtcg
     6061 gcaggggatg ggggtttgcc gtgcacgagg ctctcgacca gattggggct atcgtcgggc
     6121 cgctcctgtt cgccgccatc ttcctgctgg atggagatta ccggggcgga ttcgcactcc
     6181 ttgccgttcc attcgtgctg atgattcttg cgctcctctt tgcgcggcgc agggtgccgg
     6241 aacctacgtc gcttgagggg agtggccctg cccccggtga agccctttcc cttccccgca
     6301 ccctgctacc ctacggtgtc ttcaccgcgc tcaccatggc cgggttcgnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ccgcaccctg ctaccctacg gtgtcttcac
     6481 cgcgctcacc atggccgggt tcgcgacttt cccggtcatc gcctaccatt ttgccgtcac
     6541 cggcacggtc acggaagcag agatcccgct cttctacgct gttgccatgg gggtcgacgc
     6601 cgttgcagcg ctcgccatcg gccgcgcgta tgatcggttc gggctttctg tcctcctctc
     6661 ggtgcctctc ctcgggatct gtatccccct cctggccttc gggacagacc acgccctggc
     6721 ctttgccgcc gtggtgctct ggggtgcggc gatgggtgcg caggagacgg tcctccgggc
     6781 cgctcttgcg gactacaccc gtatcagcaa gcgcgggacg gcctacgggg tattcaatac
     6841 catctacggg gctgcctggt ttgccgggag cgctgtcatc ggctgggcct acaccttctc
     6901 catcccctct gtcatcgggt tccttgtgct gatgcaggtc ctggcgctgg gggcttttgc
     6961 ctggatgagg cgcgggttca gttcagcggc agcgtcctga gtataaaaag ggttatagtg
     7021 ctccctggaa gccctaccag ggataggggc aagagatctc taaccgggtc tttcctggat
     7081 attctggcac tatcaaagtg gtgcagcagg gggagaggga tccgggcacg catcaccgtg
     7141 cccggatctc ccgcgccacc ctgcgtccga gttcgtatgc cctggtgagt tcatcagcgt
     7201 cgggacggta gttcacatgg aaaccgccat cgatcacctc gatcccggct tctttaagcg
     7261 cctcttcaat tcgggcgacc gcgccgccca tgccgccgtg cgatccgaag ccgcaaccga
     7321 tcttctttgc cgtcaggcgg ccgggctgga ggccgcggag gtaactcaga aacccggcca
     7381 cagtggggag aacctcgtcc tggagcgtcg gcgaccccac aaggacagcc tttgagtcga
     7441 ggatctctgt gacgatatcg ctccggtgcg ttccctcgta gcggccgtcg cgcaggaggc
     7501 agacacggag gtcgacgccc tctgccatca cgccctcggc gatcgccttt gccagcatcg
     7561 ttgtcgaacc gtgcatggtg tcgtatacga tcgtgacctt atcctttgag acgccatgac
     7621 tccagtcggt gtaggctttg aggatctccc ctgcgtgcga ccgccagatg atgccgtggc
     7681 ttggtgcgat catccgtata tcgatcccga gatttccaac ctcgtcgagt ttcttcagga
     7741 cctttggtgc aagcgggatg atcaggttcg cgaagaactt ctttgcgtgc gccatcgcag
     7801 catcgatccc aagttcgtcg tcgaaccgcg cagcgcttgc gacgtgctgg ccgaaggcgt
     7861 cgtttgggaa gaggatggcg tcctcgccaa ggtaggtgaa catagagtca ggccagtgga
     7921 gcatcggcgc ttccaggaac gtcagtgtct tcccgccgag gtcgagggtg tcgccgctcc
     7981 tgacaacatg aatctcccat cctgtcgtgt cgtagtgtct ggcaagaccg gcttttgctc
     8041 gcgctgtaca gtatatcggg gtgccgggca tccgccgcat gaactctggc agggtgcccg
     8101 agtggtccat ctcgatgtgg ttcacgacga tgcagtctat ctccgccggg tcgcagaccg
     8161 acctgatgcg cccgagcacc tctttctcaa acccgtggta tgcgccgtcg atgagcgctg
     8221 tcttctcacc ccggacgagg taggcgttgt aggttgttcc cggcagggtg tagccgtggt
     8281 actccctcag gttccagtcg attgctccaa cccagtgtac accgggtgca agttctacag
     8341 acttcatctc catatcttca tctcctcttt gttcggtatt atacgaatat tctatattat
     8401 ccgaacaatt gatatatctt cgtttcgatc ttactctatg gagcctggga tgggcgtccg
     8461 tggaagaccc ggccgaccta agcctaaaca ggagatagac ctctactcaa cccctgaagg
     8521 ggcgcaggtg atcgataacc cggtgcggcg agcgatcctg gcagccctcc atgagcgtga
     8581 gcgcacattt gaggaggtcg ttttgcttgt gggccgggca aagtccacgg tctcagtgca
     8641 tctccaggag ttggctgctg ctggtatggt tggatcccgc gccgaccctg atgactcgcg
     8701 gcggaaggtc ttctacctca ccggcgatct ggtcgcgagc ctctcaccgg aggagcgtct
     8761 ggccggggac ctcgccgcct acgctggggc ataccggccg ggtatgggcg atcccttcga
     8821 gttctaccgt ctggcgttcc ggacgatccg gatcgcactc atgcatgaag ggatcgtgct
     8881 tgaccctctc ttaacccggg cgggaaaagg tgtgggcgag gaactctacc ctgcgctggc
     8941 cgacccggat atcgaacatt tctgcacaaa gattgcccgg ttctgggagg agcatcacct
     9001 gggtagggtc gaggtcgaaa gacaggatcc gctcaccctc ctggtctatg actgtttcga
     9061 gtgcgccgat ctcccggtca ccggcaggcc cgcatgcgct tttgattcag gtatcctgag
     9121 tgctctattc tcccatcact acggccggtc tgcagcggcg atcgagaccc gctgctactc
     9181 gatgggattt gaccactgca ggtttgagat aactctggaa gggtcatgat ctgtggcccg
     9241 gtcccggaca aacccctcca tcgtgctctt tgaacgatat ctcgaatctc cccgccggga
     9301 taattggcct ctccgcgcgg tctgctgtcc aggtccacca ttttatttgg ccgccggtgc
     9361 agccaccatt cctggcatga tgctggccat gaccctcccc ccgcccggct ccgggcacac
     9421 atttttatac cttgcaggac tgtaaaccca atctccttga tatgggctta aaatcagctg
     9481 aattaaatag ataaagccgc aatttgatat aaacacggtg acagaaatga agcaatcaaa
     9541 aacggcaatc cttgtcgccc tgagtctcgt ggtcatcgcc agcctcatgg ctgcgggatg
     9601 cacgggcacc ggggagaccc ctgaagagac aaccctgacg gtctttaccg ccgcatcgct
     9661 cactggagcc ttcacggata tcgccagagc ctacgaagcg gagaacccgg gaatcaaagt
     9721 cgacctggtc ttcgacggtt cacagacgct ccgtacccag atcgagcagg gagcaaacgc
     9781 cgatatcttc gtatccgcaa gtacaaagca catgcaagcc ctccagaaca gcgggttcat
     9841 ggataatgat actgtttcag acttcgtcgg gaacagcatg gccttgatag tcccgatcga
     9901 caacccggca aacatcaccg gacttgcgga cctggcaaaa cccggcgtga agatcgtcat
     9961 cgggacaaaa gatgtcccct tcggtgacta cacccgtcag gtgctcgaca aactggccgc
    10021 cgaccccgcc tacggtgagg cttaccggga ggccgtgatg aaaaatgtca tatccgagga
    10081 gacggcggtg agcaacgttg tgcccaaact ggcgctcggc gaggcggacg cggcgttcgt
    10141 ctacaagtcc gacgtctcga aggaggacct gaccagagtg gcacgcatcg agatccccga
    10201 ggattacaac gtgcaggcca cctacccgct cggcatactg gcgaactcgc cgcataaggc
    10261 tgaggcggag tcgttcatag agttcgtccg gggcccggcc ggcagtgcca tcctgcagga
    10321 ttatggattc gaccccctcc cggcagggtc gtgaggtgct gcggcacgcc gggcgtgcgc
    10381 ggttatcaag agggtttcta accctcttcg tcgccgccct tcttgtgcta ttcctcctgt
    10441 ttgtcacgct gccggttgta tcactctttc tgcggatatc gccggaagcg ttcttccgct
    10501 ctctcgccga acctgtggtt atcgacgctc tctcactctc gctcaccacc gcaaccgcaa
    10561 gcacggcgat cgtcgtcctc ctcgggacgc cgcttgccct tgtgaacgcc cggcacgatt
    10621 accccgggag ggcgctcgtc gataccctca cagatctccc tatcgtcctg ccgcctgcgg
    10681 tggcgggcat cgcgctcttg atggcttttg ggcgccgggg tgtggtcggg cagtatctta
    10741 atgagtttgg tatatccatc gccttcacca cggccgcggt tatcctggca caggtctttg
    10801 tcgcctcgcc gttctacatc cggcaggcaa gggcgagttt cgaggctgtt gaccgccact
    10861 acgaggatgc agcaaggacg cttggcgcct ccccggcaac cgtcgccctc cgggttacaa
    10921 tcccgcttgc atggggcggg cttgtctccg gtgcgatact ctcgtttgcc cgtgcactcg
    10981 gggagttcgg cgccacaatc atgtttgccg ggaactacca gggccggacg cggacgatgc
    11041 cgcttgccat ctacacgacg atgcagggtg acatggatgc ggcaatcagc cttgcgatca
    11101 tccttgtcgt tatatcgttt gtggttatcg ccgcggtgaa ggtcatcacc cggaggaact
    11161 actgatgctt gagtttcgtg ccgtccgaaa actgcgcgac ttcgtccttg atctcgccct
    11221 ctctgtcctg cccggtgaga cgcttgtcct gatcggggag aacggcgcgg gcaagtccac
    11281 ggtgctgaac ctgatctccg gcatcctcac gcccgacgag ggcgagatca cgctcggcga
    11341 ccgtgtcctc ttctcaagcg aggaacggat caacctacat ccagagaacc ggaacatcgg
    11401 ccacctcttc cagtcctacg ccctcttccc tcacatgact gtcgctgaga acgtggcgtt
    11461 cggtctccgg tgcaggaagt tgcctgaacc ggagatcaaa cgcgctgttg ctgagcaact
    11521 tgaaactatt aacctctctg aactcgcaga tgtcaacgtc ggccgcctct ccggggggca
    11581 gcggcagcgc gtggcccttg ccagggcact ggtgctcgac ccggatctac tcctgcttga
    11641 cgaacccctc gctgctgttg atatgcggtc ccaggccgca atgcgcaggg aacttcgcga
    11701 gcggatccgg aagaccggca tcccctgcat cgttgtcacc cacagcctcc gtgacgccgt
    11761 tgaactcggt gatcgcctcg gcctcatgga ggacggacat ctggttgccg gtggcaggcc
    11821 ggaggaggtg ctccggatgc gggagaacag gttcattgcg agttttgtcg ggtagcaggg
    11881 tccaggaagg ggggtttcac tatcttttgc atctccctgt cgcggacgca gtagagatac
    11941 tcctgcgcgt agccggcgta gtccccgaag tagcctcgtg caaattttcg gatccgttcg
    12001 cagactgccg gggtgcaccc cctcccggca aggtccggga gatatgcctc tgtcatgatc
    12061 cggtggatcc agacgtcgac cgggaacgcc tcaaagaatc caaacgcaaa gagcagcacg
    12121 cagtccgccg ccttcgggcc gaccccccgg aagtgcatca gcgcttctcg tgcctcctcg
    12181 aacgggaggg cggcgatccg ctctgcccag tccgggttct ccgccgcata cagggcggcc
    12241 tccctgaccg catccgcccg gtaaccgacc ttacactccc gcaggtctgt gcaggagagc
    12301 tccgccagtg cgtccggttc cgggaacgca tacgccctgc caaacggtcc ttctatcggt
    12361 ctcccgtaac gctctgccat gagcgcgatc cgtcgcttca cagcagggat gttcgtgttt
    12421 gtggcgcaga tgtaggagac caggcactcc cagggaggtt gcctgaccag ccgcagaccc
    12481 atgcattccc ggatggcagc gccgatcact ggatcgcgat cgatcgaggc gaggatggac
    12541 gggagatcct ggtcgagacg gaggtagtcg tggataaact gtgcatccgc cccgctaaac
    12601 agaagacggt ttccgtcctg ccgtatccgg atcgcctcct cgccgacgac gccgttccac
    12661 caaccgtccg cctcctccca gcggaacgcc tgcccgcagg agagcgtacg gtcgaggtct
    12721 aacggctggt caggccggag caggatcgct gtcatcgccg gggtgcagaa aaattcaggt
    12781 tattcggcct tctcatcctt catctgctgg tagacgcagg actggacgtc ctggccgagt
    12841 ttcccgatcg cgtccgcccg gcagcgggca cagtgtcgca tctgtttgat gtagggggcg
    12901 cagcggtcct gcatatctcg cttctccgcc ggtgtcggtg gggttatatc agcgaactta
    12961 tactggggta tgagcgggat gacgttgaag gtgaacgctc ccatctcgcc ggccttcttt
    13021 gcaatctccg ggatgtgatc gtcgttgatc cccgggatgt agacggtgtt gatctttaca
    13081 aacatcttct ttgcaacggc catctcgatc cccctaagtt gctgggagag caggagttcg
    13141 gccgcctcgc gcccatgatg cctcttcccc tcatactcga cccaggagta gatcttctcc
    13201 ccgatcgcag ggtcgacggc gttgagcgtg accgtaacgt tcccgacatc atatctctcc
    13261 aggtcgtcga tcgattcggg gagcagtagc ccgttcgtgc tgatgcacat gatcaggtgc
    13321 gggaactcct cgtggacaag ccgcaaagtc tcgaacgtct cggggtttgc cagaggttca
    13381 ccaggtcccg cgatgccgat caccttgaca taggtgtagt ccctgatgac ctttctgacc
    13441 aggtcaagcc cctcctccgg ggtgagcaca cggctcgtca cccccggtcg gctctcgtta
    13501 acgcaatcgt agtcccgcac gcagtagttg cactggatgt tgcatcttgg agccacgggg
    13561 aggtggcacc gcccgaaggc gtggcatgct ttatcggaat aacagggatg ttcgctgatt
    13621 ttacgcagtt gctccgggtc gtaaggaacc tccctgccct ggactgtcgc ggtccggtac
    13681 tcgtcggcca tatgatatga tctcagtggc gggagttata tactgatggt tgccgccact
    13741 caggaggtgg aggttctcaa accagctccc ccctctggaa taacgtccca cacaaaatcc
    13801 agaccacacc tcgatgcaga tagggggtgg tctgcgacta tgcgcatccc cctggaatcc
    13861 gcctcgggat agggtcatca aaatcggggc acaccccatg ggtattgatc tgacggcctc
    13921 ccagggatca ggccacctcc ccggcatcaa gcggcaactg ttccgccctc tcttcctccc
    13981 gtgccccgtt ctccggttcc atcccgaacc gctcctcggc agccttcagg atccggccga
    14041 gcatcccgct gtatgagaga cccgcgtact tcgccatctt tgcaaggtgg ccgtcccagc
    14101 accagccggg gttcgggttg acctccagga gtttcgggtt gccctgagca tcgagccgcc
    14161 agtcgaaccg gcagtaatcg cggcactcca gacgttcgaa gagtgagatg cagcacctca
    14221 cgatcagttt ctcggtaccc tccggtagat tcgcaggttt cgatgtgatc ttccagtagg
    14281 gcgagtccgg gagccacttt gcctcgtagc cgcatatgcg gggaaggtcg ggtggcagcg
    14341 cggagtagtc ctcctcgatt atcggaagca ccgtgcatgg tcccgggggg ttcccgatga
    14401 tcccgacgct tatatcctta ccggtaagga actcctcgac caggagcgag cggtcgtagc
    14461 cgagcgtcgc tcgcagggag ttgatgacat tggagagttc ttcgatggtg tgggcgatgc
    14521 tcgcagcggt gatgccataa gaggagtcac ccgtgttcgg cttgacgatc accgggaaat
    14581 tcacccccac ctcatatgtc cggtcacccg gcgcgataaa gcatgcctcc ggcaccggta
    14641 tccccatctc ctttgcaatc ccgcgcacga gggatttatc gtagcagaac gcgagcgact
    14701 gcggcccggc tcctgtatag gggatgtcca gcatctcaag aagcgccggt atgtggagtt
    14761 cccgcccagg gtcgttgttg aaaccctcgt cgcagaggtt gaggacatag tccacccgtc
    14821 ctttcagcct caggatatcc cggatcagcg tctcgtgctt gcagaggtag gtaaaccggt
    14881 accccggcaa ttcgcggagg gctgccttca tgcgatcgat ggtgtagatg tcatcgtcgt
    14941 caaagacgca ggaaggtttc aggggatcgg gtttgtccgg gtcgcctagc agcaccgcta
    15001 cgtgttttgt ccttccctgt cctcttgccc tcgtcgtcga ccattctttc cttatctgag
    15061 cggtgacgat gtatcggcgc tccatcatgc cgagatcctg gttgcgctct gactcagtgg
    15121 ctatctcgtg gaaggcgatc tcggtgaagc ctgcccgtac caggagcctc tgaagcgcat
    15181 cgggggtgta taggcgttca gcataaaact ggtcggccac aacacccttc tcaacgtcgg
    15241 tgataacctc gcgggatatc aggcgctgtt catccagcga gagcgatcgt tcgcggcaga
    15301 caaacatccc ggtgtcgatc cactcccacg acctcggctg gaagtgcgct ttgagatagc
    15361 tgccgtcggc aacatccagc agcagccgtc cccagggctt cagaacgcgc cggagttcgt
    15421 tgaggatctg catatcctcc tcaacggaat cgaagtagcc aaagctgttc ccgaggacga
    15481 gggctacgtc gtaggtgtcg ttgcggtatg ggagcctccg tgcatctccc tcccggaacc
    15541 tgaccgtaag cccttccttc tttgcggtcg agcgcgcgcg ctggatcagg tagtgcgacc
    15601 ggtcgagccc ttcggcgcta taccccctgc gcgcaagttc cagggtgtgt ctgccctggc
    15661 cgcagcagag atcaaggatc ttatcatcgg gctggaggcg caggatccgg atgatcctgt
    15721 cgatctcact cgctgtgatc ctgggatctt cgacaacgtc gccatcggtc ttgaggtaga
    15781 ggttgttgaa gatacccctc caccagtcgg gctttacata catctccaga ttcgggaccg
    15841 ggcctagtga ccttctatcc cggtgttttc ctctccgttt gctatcggga ggttcatcgc
    15901 catttgcctc gccgttcatg cctgtcaacg cctccaggct ttatttccgg aataggaccg
    15961 gtatcccatc tggttacaaa agagaacagt tgtggtctgc atatctggat gatcattcga
    16021 tcgtatggta tcgataaaag tgttgtgcca tggttccatc tctcctgcaa aataacccca
    16081 taaatggggg ttcttagcaa tgctcctccc ggttacgcac tgccagcgca cgggggttgt
    16141 gccatggact tccgggctgc atctatatat gcaggcaggt tgtttgaata tgtgcagatt
    16201 atctgcatgg ttgaagtata tgaatgagag atctccccgc aaaggacccc gttcatttca
    16261 ggatcgccct cgcgagtttc acaaagcggt ctgttccgac tgcggcaaag agtgcgaagt
    16321 acctttcaag cccacggagg gtcggccagt ctactgccgt gactgcctcc caaagcacag
    16381 aaaacccaga ttttaacttt ttttatccga tctccctaaa ctacaagccg cctcctcatc
    16441 agtctgatgg ccaggctgca gaagatcgcg gtcgccactg ctatccaggc gaggttgagc
    16501 aggtttgccg ctgaaaccgc ctcgagcgtt atcgcccggt taatctcgac aacatgagtg
    16561 agcgggaggg cggccagggc agcgcagcgg accgcatcgg gaaggagttc gagcgggaag
    16621 aacgtcccgg agaagaggaa catcggcgtg atgaagagga atgaggggta attcagcgtc
    16681 tcgatgctcg gggtgatcgc tgtgaaacac atcgcgatgc ctgcaaagag gagtcctgca
    16741 atgaacgcga atgggatgag gagcagggag gagggcatgg cggcaacacc gaagagtatg
    16801 agcaccggga gcatcagccc ggcgtagata acgcctcttg tggcgcccca gatcatctct
    16861 cccgcaatca cctcgtcgat gctgaccggt gttgcgatga tggcgtcaaa cgtcttctgg
    16921 tagtacatac gtacaaacga ggagtaggtg cactcgtaga aggctgagtt catcacagat
    16981 atcgagacca gggccggtgc gatgaatacc gggtagggga cgccgtctac cgcctctatg
    17041 taggtcccga gcccgaaccc cagtgccagg aggtagagga gcggttcgat gaatggaggg
    17101 atgaagttta cacggtatgt cctgaggaag gcctccaggt tcctctgcca gacgctaccc
    17161 accctctgga tacactcaat catcgccgcc tcactcccgc agcttccgac ccgtgaggcg
    17221 caggaagacg tcctccaggg ttgccggacg gacggtgata gccgcctcgt gccggcagag
    17281 atcgagcaac tcacccacaa tctcatcggc gcgatcggta aagacctgga gcatatcgcc
    17341 ggcaacgtcg taggccacgc cgagtttgtc cagaagggct gcagtctttt ctgtccgctc
    17401 gatctctatg acctcgtttc cggcatactc gtggacgatc tccgccgggg cgccctcgat
    17461 caggatcctg ccgtgatcca tgatcacaag ccgatcgcag aggcgttcag cctcgtccag
    17521 gtagtgagtg gtgagcacaa gggtgtttcc ctcagcccgg agaccacgga gggtctccca
    17581 gatcaggtgc cttgcctgcg ggtcaagccc gattgtcggt tcgtcgagga ttagcagttc
    17641 tggttcgttt acgagcgcac gggcgacgat cagccggcgt ttcatcccgc ccgagagttt
    17701 ctcgatcagg acgtcccgct tctcctcaag gcgcatgaac ccgaggagcc tctccgcccg
    17761 ctcttctgcc acactctttg gtataccaaa atagcgggcg tagaccagca ggttccggta
    17821 tgccgtgaaa tccgggtcga gattgttctc ctgcggcacc acgccgatgc ggctcttgat
    17881 ctcgcggggg tgggtgtcca cgtccatgcc aaagacctcc aggctgccgc cgctctttgg
    17941 agagatgcac tggatcatct tcatggtcgt cgtcttcccc gctccgttag gacccaggaa
    18001 cccaaagacc tctccctccc tgacccggaa cgagacacga tcgaccgcgg caacgtcgcc
    18061 aaaccgtttc tccaggtcgt gtgctgcgat gatatcccct atcctctctc cctccaggat
    18121 aagagatggg tttttttgga tacaacattc tttcgctctc cgagcctctc tgtgaggggt
    18181 gcgcggggcc gcactctgca ccgctggtag atagagaatc tggtcgggat cccgggtctg
    18241 ccatgacaga ggatgcatac ccattttact ggagagatcg caccactacg ctgcaagcga
    18301 taccatgacc gatagtgctg agatggggag tgtaatttat cctccagata ctgtgtatgt
    18361 cgctatgtca aagtgccatg ggcggggagt cttcgcccgc agggatcttc tggccggtga
    18421 ggtgatcgag gtctgcccgg tgatcgtgct tggtggagca gacgagcagg aactcctgga
    18481 taagacgcac ctctttgatt actactttga gtggggggga cttgctgcgg tggcgcttgg
    18541 atacggttcg ctctacaacc actccagcca cgcgaacgca gaccacgtct gtgacgttca
    18601 tcggggtgag atccgtatct atgctcaccg ccacatccgg cagggtgagg agatcacgat
    18661 caactacggt ggccggccag actgccccga ccccgtctgg ttcgatgcgg tggagggatg
    18721 atacgctaaa gtgccggggg tgatgcgggt ccgtcatatg ctcgcagtcc agtcgtggca
    18781 tcaagaacgg gtctgtcccc ctgtctgaaa ttctcaatgt ccggcacctt gcgtctccct
    18841 ggtgagacac atcttccagg aggtggggca ggcatggagc gggagtggca gtgggtgaga
    18901 tggaatgggt accgcgtggg cactggcccc catcaggcgg gcagttcacg gctattgtgt
    18961 aatcctcccg gtctgccagg gcaccaccct gcgttgaacc cgaaccactt gctccagagg
    19021 agcgcccccc ctgaagagaa ttaccgttgt gttgtccggt accattgcat gctgtggcag
    19081 ggcgaacccg ggttgcctcc cgatgatcag ggcagatgca agcgcagaaa accgcgtgga
    19141 gagattggca agcctgccgc cggagtgagt gcaagaactc tttcgcggta tatgggagga
    19201 gacttcattc gatggatcac tattcctctc tggtgttatg gggagaggac tgatacagtt
    19261 ttgacggtcg aagtataact taatgttatg ggcatggttt ggggagaaga gaacgtacgc
    19321 cgggtctgcg cccgtctgcg ttcagcaaag acccgtgcag agcttgcagc gatcctctac
    19381 caggaggtat cccgctactc tctctctgac ctgcaggcgg tgcggggtag ggttgagcgc
    19441 gacctcaggt tcgtcccacc cgcctaccgg cagcggctgt atccaaggct tgtggagcag
    19501 gtctttgata cgcaccatgc cctcatctcc accattcgcc aggggaggct tcgggtcggg
    19561 ggagaaccgc ttccccctga atacgaggag ttctgcgata tggtcgagag gacgtgcctt
    19621 gccggagaag aggatgaaca gcacctcgag ctcctctact tcctccttgc cgcctttaac
    19681 atctttgtcc tcgacctccc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnagctcct ctacttcctc cttgccgcct ttaacatctt tgtcctcgac ctccccgctc
    19861 acccggtagg gactccgttt cccggcgggc tcaaggtcga ggcaaggaac ggagaatacc
    19921 tctgtcccgt cagggagaag gccaacgatg tcgagaacgc tctctgcccc tactgcccgg
    19981 caaaacagag cgagatctga gtcctgcgca gggtctatcc cctttcgttg caaaaccggt
    20041 tttggaatga aacctccagc cgcaccggta ggcggatggc ggtgaggcgg tcctgtggtg
    20101 atgcagattc cgccgaaacc cgtacaggga gtgtttcatc aaaccagggc acagcctgaa
    20161 aaagggtgat ggcctggcag ccttgattta cttctcttct tcgtatcgat cgaagaatgc
    20221 aacctcgtcg aggggtttct tcttcgcgat cgatatctcc tccgggtatc ccgccggcac
    20281 cagcgagacg agggtgtagt cttccggaac gttcagaagt ttccggacat cctcggcata
    20341 gtccttcttt gcccccgcga cccagcatga cccgacaccc catccctgga gcgccaggat
    20401 gagctgcatc gttgccgcgc agcagtcctc caggtagtat ttagcgtcct tcttcccaaa
    20461 gactgcaaaa cagattggag cattggcgat gaaccggccg ttatcggcca ggcccgcgat
    20521 cgccttcaac gtatctcggt cgcggatcgc cccgaagagc cagggttgct catttcttgc
    20581 ggtcggggca aggtgcgcgc agtcaagggc gtctttcaca atcttatcat cgataggagt
    20641 gtctttgtac ttccgcacgc tgtgtctgct ccggatcacc gtgacagcga ggttaaccgc
    20701 tccaccaatc gagctcatac aacgattgtg ggtattccac gtctaaatac cttggggaac
    20761 aggattggat gccgcgcacc cgcggcaggt gcgggtccta gaggagaccc gctatcgcct
    20821 gaagcatatt cgagaaccca tccccgatga ccatggcggg gtcgatcccg aggaccggga
    20881 agatgaagat gaagtaggcg aaagtcccga tcgaactccc gacgttcgcc agagccgcaa
    20941 ccagcaccac ccggaatagc gggatccgcc gcatctcgga gaaggtctcg gcctccatta
    21001 tctgcctgat ctccccggag gcgggtttcc ttatcttcgc ctcgacgata gccgcaaacc
    21061 atcccgccgc aagcagcggg ttgagcgagg tcatccagga gacggcaaac gccgtggcgg
    21121 ccgagagtgg gtgcccgccg gctataaacg tgaatgcggc gctcaggaga ccgttgatca
    21181 gtatccagta tatgatggcg gtcagcagga catccagtcc aacccccgag aaggctatcg
    21241 cggcgatcag gagaagaaag aggatggtga cacccgctcc caggatcttt gcccacggca
    21301 gtcctttctt cacttcggcg gtcaggtcag ataacagcgg cagcgtctcc ggggcgtcga
    21361 ggtagcgctg cacccccttg atgtgcccgg caccgatgac cgccagaaca cggtcgtagc
    21421 gcctggcaag catcaggatc tggtgggcaa ggtaggcgtc ccgttcatcg atcagtgcct
    21481 gagcgccccg cggggagaac ttccggaact cctccatcgc gacgctcacc acatcctgat
    21541 ccgtgagttc gtctatctca atctcctgcg tatcgacgct cacaagcgag tagaggagcg
    21601 cccatatgag cctgatcttc tcccggatcc ccatcagacc ccagaatcgg gagagcgtga
    21661 tccggatatc gcggtctatg aaagccacgg gtttctggtg tgcgcgcgcc tcctcaatgg
    21721 cggcaagcat ctcggaaccc ggtttaaccc ctgtctcagc gccgatacgc tgctggatgt
    21781 atgtgagtat ccactgtagc aggatctggc cgaagttccc gctcttcagg atctccgtca
    21841 ccgagggttc ggccgtctcc tcggtgagtg agatgaacct gccccgatcg agttcgacac
    21901 ccacgatatc gggctggaac tcctctatgg ccgatcttac ctcctcgaca ctcttctgcg
    21961 agacgtgcgc cgtcccgacg agacggatct cagccatcga gcaccttcat gccctcacta
    22021 tcgatcacga ccctctttcc cggtggaatc tccgcctcca gaaagatttc gcggatctct
    22081 gcctcctcct cggcctccgc ggcctggcgc ctgcagagat cggcagcagc atcgcgtgca
    22141 gcgctggggg atatggaagc gccgagaccc gggcattccg agaccatcag gtcatcatcg
    22201 accagcatga aagggtaggt gcggcagatc cagggccgat gctcatagat cgagcaccgg
    22261 ccatccttga ggaagataca cgcatcgagc gttcgccgaa ggcaccaggc cagggtgtac
    22321 tcaccaccgt ttcctgctct gataaactcc gggtagggct caacaatctc ttccctcttc
    22381 atcccggtag cagccatgat ctcacgaagt tcagcggcgc ccaccagcac aaggtttgag
    22441 tcttccccga ccctccggca acacctgcca cactctctgc agcagaagcc tatcgacccg
    22501 agtcgttctg cgatctcatc tactccgagc atatcctatc aaaattctcg aataaaagtc
    22561 ggccgttaaa agtatggctg acttccgggt gccactggat cccgtagagg cgttcttctg
    22621 gagagaccat agcctccaca tcgcagatgg ccgatcttgc aacccggaga aacccctccg
    22681 gcaccaccga gacctcgtct gcatgcgagg cccatacctg catcctctcg gggtagccct
    22741 ggaggagagg atcctgctcc aggacctcca cctcaacccc cccgaaacct ccgctcgcgc
    22801 cccggcggat cgcgcctcca cgcgctgttg cgatgatgtg cagcccgagg cagatcccga
    22861 ggaccgggat attgaggtcg aggtagtcgc cgctgagacc ggcgcgttcc agggtgggtc
    22921 cgccgccaag gatgattcca cggcagccgt tggcgacctc gtcaggcggc gtcatgttcg
    22981 agatcatccc ggcctctata tccatatcgc ggagtgtccg gaggatcagg tggttgaact
    23041 gtccatggtt attgaccaca tagagtggaa gcattgctta ataaatggac gtaaaagggt
    23101 aatagtcctt ccctcaggcg tacttcatta caaaatcgaa tacttttcgg gagatctcct
    23161 ccggcctgtg gcccatgagg tatgcaagca ccagggctcc gccggcaccc gccccttcct
    23221 tcacctcgcc gatacagtat cgggcaagtc ctgggtggcc gatattttca aagttcgggt
    23281 cgacgtagta ggcggtggtg ccaacttcgg ctacggaacg gctgaaccct gcggaggggt
    23341 cgtccctgac atagaccgtc gtcgcaaggc gaggcaccct ccgtcccagg gctttcagga
    23401 cggccgcaac ggccagcatc tgggttccgc cggcaaagat tagatcaccg gagtatgtgt
    23461 tcacgatccc tgcagcgact ggcatcatgg ggtcgccagc ggcgcggagg atacccatgg
    23521 gctcttctat gccattctcc cgaatcctgg cgagaacttc acgacagacg ccctctttca
    23581 ggtcaatggg gtttctcggg aaggcgctgc tgaccgatgc gtcgtacccg agcgcccgca
    23641 ggacgcagag ggcggtggtc gtcccgccgg ggacgcactc gccgagcatc aggaggtcgc
    23701 tgtagtgcga gataatccgg ccgatcgctt ctccccgttc gaagaggagc ggcgcatccg
    23761 gcaccgcatc ctctatgcgg ggatcgccgc cgggtctccc gtagacgtcc aggcagggga
    23821 cggtgggggt gtgcgcaagc ccggcgttga tgaagatggg ggatagcccg gtaagttcca
    23881 tcatcgaccg ggtgatggag gccggtgtcg ggcaccccgt gggggtgttt ggtctggccg
    23941 gcatgcttgt gatcgcgccg gtagtgatga gttctgcatc cagaatcggc gttagcagcg
    24001 tcttctccgg tgtcggccct gcacccgata taccgggaac ggttgacagc atggtgtttc
    24061 caagcaccac caccatcatc ggtcggcgtg gcctgaatcc cgggtcttca gagaggaagg
    24121 gatgagacat tgtgtagata aatatgtgct gccggataga agatgttgct gtcgcgccgg
    24181 catctggagg cggttgaaag gctggcagcc tcaaggactt atcggcttga taccaaacgc
    24241 caaatacttc ccgacccaaa taccacaacg atgtttgaga tagagcagag actggaaggg
    24301 aagggtatcc tgcgcagaga tatcgtcgct gcggcgatgg aactctacat cccccacggg
    24361 atagatgagg atgaggccgc acgcaggctt gatgcgaaga tcgggcaggc tctcgatgac
    24421 ccaaacgtat cctcgctcct cctcggcgcc ctcctgcttg aggacgaact ctatacgaag
    24481 cgagagaact cggagatcgc tgacgatcct gtctttttgc tctcagacga gataatcggg
    24541 atggccatcg ccgaggtgat tggggggaca tacgcgcggt tcgagttcac ccgctatgac
    24601 cagaaaaaac caggagtctt gaaggaactg ggtcctttcc ttgatgatgc cgtcgccggg
    24661 ctgatcgcgg gctgcacctc ccgcctctac agtgagtcga tgggatcggt gtgagatcgg
    24721 ttcttgccct gctgcagttc tgtaccaccc tccccctcgg gagggtggtt gatttcgagc
    24781 acttcgcccg ccgttcctac ctctacccca ttgccggtta tatcatcggc ggggtagcgg
    24841 ccattgtcac gttctggata gcatccccat ccgtgggagc ggccgtcgcc atcgccgcgg
    24901 tgctcctcct ctccgggtgc aaccatttcg acgggctgct cgatctgggg gacggactta
    24961 tggcccacgg cgggcgggag aaaaggatca cggcaatgac cgatcgggca acaggggcgg
    25021 gcgctgttgc ggcggggatc ctcgtcacgc tccttgcgtt ttccgggctc caggccgccg
    25081 caaacattcc tctaaccata cttaccgccg aggtctgcgc gaagaccgcg atgtcctggc
    25141 tcaccactct cggtagaccg tttcgcgagg gtatccattc ttatcttcat agttttgcaa
    25201 aaccctggtt tctggtcccg gcatttctgc tcacgctgcc gctattcctg ctgccgctgc
    25261 cgcggctggc tctcgtgctt gcccttgcag ccaccgccgc catcaccgca cttatgcttg
    25321 tgctctcccg gagggtcttt ggcggggtaa atggggatat cgttggggca tcaaacgaga
    25381 tagtccgggc gggtgtcata cttctccttg ctcttgcatg accatctttt ttttatcctg
    25441 gtgttctcca ctactgtaga atgatgcagg aacagagcct atactcccgc ggggggatca
    25501 tcaccgaacg tgatgccgag tatgcaacgg tgcgggttcg gattccggca ggtgttctct
    25561 ccgcggcgca ggtcaggcaa ctgggaaaga tcagcgagaa gtatggtgac ggcacgctcc
    25621 acctcaccat gcgccagacg gtggagatcc cgcacgtaaa gccggaaaac tttgaaaagg
    25681 ttgcaaaagc cctcgaaaag aacggtacgc cgcttggtgc ggagcagaac gaggttgtca
    25741 acatcatggc ctgcccgggt acggagcgat gcaagtacgc taactgcgag actatcgacc
    25801 tcgccagaaa gattgatgag cgtgtcttcg ggaaggtact ttctatccgg cttcgtattg
    25861 ccatatccgg ctgcccctac atgtgcaaca gtccgctgct aaacgacatc gggatcatcg
    25921 gcaggatccg tcctctgcgc attcccggcc tctgcaccgg ctgcggcacc tgcgtggagt
    25981 actgcaaaca gcacgccatt gcccttaaaa acggtgtttc agtccttgat gagagcaaat
    26041 gcgttcagtg cggcgtttgc atacattcct gcccatacca tctcctgaaa tcggagtatg
    26101 accattacca gatcacggtc ggcggtcgac gcggggcctc tcctgccgca gggcgtgaac
    26161 ttgttacagt cgagaccgct gaggaggtgg ttgaggtggt cgaccgcatc gtttactggg
    26221 tctaccggag tgcctggagc ggcaggccgc tggccgatca gatggatgag atcggatatg
    26281 ctaagttcag agaagagatc cagaaggagt tcgggccgaa accctcagaa gagaagcgct
    26341 aactggagaa gccgatagat atacgatatg gaaaggaaag gagcaggtcg gggggctagc
    26401 cccttctaac ggtagtacat cgaggcgaac ccctctccag cctcgtcctc gcattcaata
    26461 ccggtgccgt cccgaacgag tgagttaaac atcatggccg cgtttaatag gttctcgtcc
    26521 gggtttgagc catgccggac gccactcagc gcctgacgct gtggtgtcgc cattgtccgt
    26581 ttacccaccc ggacaccagc acagtagcgg cagtaggcac agtgtgagta gaccgatacc
    26641 tcccggcccg cgcaggttac agtcaccctg tcctttgcct catcacggtg aaactcaagc
    26701 aacttcatgt atgaggaaca tagactctca agaaataaga ttatgtcgac tgccatgagt
    26761 ttttgaagca gtggcggcac tgaagcgcat agaaatgctt tatatggtag acgtcacacg
    26821 tataagttac tcgcataagc aggctgtttg agctgacagg cctatatgag tggaggatat
    26881 aatctatggc agttgaaaag ccgcacctaa acttagcagt tatcggacat atcgaccacg
    26941 ggaagtctac caccgtaggt cggctgctct ttgagaccgg agccgtaccg ccccacatca
    27001 ttgagaccta caggaaagag gccgaagcca agggcaaggg ctcgtttgag tttgcctggg
    27061 ttatggacag cctcaaggaa gagcgtgagc gtggcatcac catcgatatc gctcacaggc
    27121 ggttcgatac cgaaaagtac tactttaccg ttgtggactg ccctggtcac cgtgacttcg
    27181 tcaagaacat gatcacgggc gcgtcccagg cggacgctgc gctcctggtt gttgccgcgc
    27241 cggacggtgt gatggaacag accaaggagc atgtcttcct ggcccgcaca ctcgggatca
    27301 atcagctgat cgtcggcatc aacaagatgg atgccgtcaa gtatgatgag aagcgtttca
    27361 atgaggtcaa ggcagaactc tcccagttgc tcaagatcgt cggttacaag cccgacaacg
    27421 tcatcttcat cccgatgagc gcgttcaagg gtgacaacat ctccaagcac agtgagaaca
    27481 ctccctggta taagggcccg acgcttcttg aagcgcttaa catgctcagc gaacccgaga
    27541 agcccaccaa ccttcccctg cgtcttccta tccaggatgt ctactccatc tccggtatag
    27601 gaaccgtgcc agtaggccgc gtcgagaccg gtgtcatgaa gaaaggcatg aaggtcagtt
    27661 tcatgcccgc taacaaggag ggtgaggtca agtctatcga gatgcaccac gaggagattc
    27721 ccgaggcgct tccaggtgac aacgtcgggt tcaacgttcg cggtatcggt aagggtgata
    27781 tccgccgcgg tgatgtttgc ggccctgctg acgcaccgcc gaccgttgcc gaggagtttg
    27841 ttgcgcaggt tgttgtgctt caccacccca gcgccctgac cgtcggttac acaccggtct
    27901 tccactgcca caccgcccag atcgcatgca catttgtcga gctccagaag aaactcgacc
    27961 cccgtaccgg tcaggtcaag gaaacgaacc ccactttcct caagaccggt gatgccgcga
    28021 tcgtcaagat caggcctacc cgtccaatgg tcatcgagaa ggtcaaggag atcccgcagc
    28081 tcggacggtt tgcagtccgt gatatgggct caaccatcgc ggcaggcatg tgcatcgaca
    28141 ttacaccaaa gcagatgaga taagaagaat tactacattt ttggtggtta cagatgcaga
    28201 aggccagaat acgtctttca ggaaccgact ttgagaagat cgagatggtc tgtgacagga
    28261 tcaaagagat tgcagagcgt accggcgtca atctggcagg tccaataccg ctgcccacga
    28321 agaaacttgt ggtccccgcc aggaagagcc ctgatggtga gggtactgaa acctgggatc
    28381 gctggcagat gcgggtgcat aagaggctca tagacatcga tgccgacgag cgcgcgctgc
    28441 gccagttgat gcggatacag gttccaaaag acatcggcat tgagattgtg ctggagagtt
    28501 gaggtgggtg cggcgtgaag gccgctgcgt tcacggcgag ggtccgtgaa aatctctcat
    28561 tagaggtggt tttcctcctc atatttctgg ctgcagttgc ccttcgtttt tatgctcttg
    28621 acttaaagtt gttccaccac gatgaggccg ttcatgcgtg gtttgcttac aatctcctga
    28681 ctgacgggac gtatatctac gatcccatgt atcatgggcc gttcctctac tacgtgacgg
    28741 ccggggtctt ttcgctcctt ggtgactccg atctcacggg aaggctcctc cctgcgctgt
    28801 ttggcagctt gcttctactg ctgctttacc ccatctacag gctcggctac ctggataaga
    28861 aacagaccct ggtagcggcg ctattccttg cggtatcgcc gaacatggtc tacttctcgc
    28921 ggttcctgcg gaacgatatc tttatagcct tctttacgat gctcctcctt gtcgctctgc
    28981 tctactactt tgagcggggt cagacaaggt atgccctgct cgctggaaca gcgatagggc
    29041 tgggaatgtc tgccaaggag aacatgccca tcgttatcct gatcttcggg ttatacctca
    29101 tctacctgat ctggacgggg aaggtcaggc ttccggccca ctgggtcagg gatcttgtgc
    29161 tcggcgttgt cgtcgcggtc ggtattgcga cggtcttcta ctcctcgttt ggcgcccatc
    29221 ctgagatggc cctggactgg tggctccggg ctatcgagca ctggacggcg atgcacgaga
    29281 tgcagcgtat cggggggccg ccctacttct atatcctgct cttcatcctc tacgaggcac
    29341 cgatcctgat ccttgccatc gtcggggttc tgcagttttt cgatgtcgcg ggcataatct
    29401 ccagttggag agagcgaaga caggatatgg agaagcccgg tgagaccggg gtaactgatg
    29461 ttgacaatgt tgacaaagaa gagacccctg accctctgcc cccgccgccg gaggtggcgg
    29521 cccggggcct gagggggcgg cttcgcgata taatctccgg gagcggggag atccggcaga
    29581 tcgatcggca gaaggagttt gcaaggttcg ccatattctg gatgcttcta tcgcttgcgg
    29641 catacgccta catcggcgag aaggtgccct ggctgatcct ccaccagctt ctgcccatga
    29701 tcttcgttgc ggtctatcag atgacaacga agaaggcggt aatcgccgtc gtggcgagca
    29761 tcctcctggt cgcgatgacg tttcacgtgg cgttcacacc ggcagacata aacgaaccca
    29821 tagttcaggt ccagaactcc gaggacctcc ggtggctctt tgcaaagatc gatgccgccg
    29881 accgtgtggc gatagcaacc gattcctact ggccgctgcc ctggtactac cggggcgacc
    29941 ggagttcaaa aatcgcttac tatggccaga aggtgagcga ggagacgata tacagcggga
    30001 actttgacct tgttgtcact cacgatgctg atacctaccc ggcgctcgat ggttacgtta
    30061 aggagacctg caggttgaac tactggttct catacgggga gaacaaagac aggttgcttg
    30121 cctactactt cttcagggac ggaaaggtcg gcagcagaat actcgaggta ttctcgaggg
    30181 cctctgcggg gacggccacc tgatgctctt ctctcccccg ggacgccgtt gatggcgtga
    30241 ggctgtcccc tggtgtcaac ggcggcgtaa ctttgtgcac tttcggcggt ctaaaagtgt
    30301 acacc
//
LOCUS       DAOSZX010000007        30119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1264_length_30119_cov_19.825373, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000007 DAOSZX010000000
VERSION     DAOSZX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 30119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30119
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_30119_cov_19.825373"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK196_01305"
     CDS             <1..482
                     /locus_tag="PK196_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HON40095.1"
                     /translation="DIRPCRGCNACERLNRCVQRDYMDYAHDRIIAADCIILASPIYC
                     MGLAAQAKILVDRAQVFRSRKYVLHLPVVPPERKGKRVGVFLSTAGQNWENVFDAAIP
                     SVKCFFQVVDIPNRDIRYLMINGVDEKGAIERHPTARDDAGRLAREVAARLREVRAA"
     gene            479..1114
                     /locus_tag="PK196_01310"
     CDS             479..1114
                     /locus_tag="PK196_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON40096.1"
                     /translation="MRRPIKVLGISGSPRRHGNTETLLDAVLDGAAEAAGSIEKVILR
                     SLDYASCRGCNACHKTGVCVIRDSLTPVFDKIADADVLVLASPIYSMGITAETKGLID
                     RTQFLWARKFILKNLYYPANQIRRRKGIFVSTAGLGWENVFDAAFPAVTAFFNTTGFD
                     YWDNIIANDLDRYGGIEGHPTALTEAHEKGRRVVELLQRMEEESLSSAPGT"
     gene            complement(1165..1749)
                     /locus_tag="PK196_01315"
     CDS             complement(1165..1749)
                     /locus_tag="PK196_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HON40097.1"
                     /translation="MLERIPPRERRDLLIAWLAISIAFTLIYIRGGVNVAELVFFFVM
                     SLITVGVAFVLHELAHKFSAMRYGYWAEFQKDNQMLLVAVVMAALVGIVFAAPGATYI
                     YGNATRTENGRISAAGPVTNLLLCIPFAALMLLGGELLAIVGLVGLRVNAMIATFNML
                     PISILDGRKVLAWNPAVFAVLMAASLGVLVWSLL"
     gene            1846..2130
                     /locus_tag="PK196_01320"
     CDS             1846..2130
                     /locus_tag="PK196_01320"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HON40098.1"
                     /translation="MSLDAVRNEIREIDEKIIDLIAERQNLAAEVARIKREMGLPLRD
                     EAQRRVVLDRVFAYAAESRIDPLAVRRVFETLVEMSEERQRECSGDGNLP"
     gene            complement(2141..3010)
                     /gene="argB"
                     /locus_tag="PK196_01325"
     CDS             complement(2141..3010)
                     /gene="argB"
                     /locus_tag="PK196_01325"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HON40099.1"
                     /translation="MKREDVLMEALPYIQKFYGKTIVIKLGGHAMVDQKILETVIRDA
                     VLLHYVGMKVVLVHGGGPEITAKMKAMGKEPKFVGGLRITDPETLEIAQMVLVGKIND
                     GIVSLIANCGTRAVGISGNDGNLLIARKMEPQRVQIGETFEEVDLGHVGEIEEVDPEV
                     LHCLLAQNYIPVVAPIAIDRQGRSLNINADTAAAEIAIALKAYKLVNLTDVDGVMDAD
                     RKKVYHRLSLADAEGMIERGVIAGGMIPKLEGCMKAVRNGVTSAHVVNGNREHNLLLE
                     LFTDEGVGTMITP"
     gene            complement(3007..4146)
                     /gene="argJ"
                     /locus_tag="PK196_01330"
     CDS             complement(3007..4146)
                     /gene="argJ"
                     /locus_tag="PK196_01330"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HON40100.1"
                     /translation="MKGICAVEGVSAAGMKDGKFGLALIQASGTASGVFTSNLVRAAP
                     VELMMERIRRGALEAIVVNSGCANAYTGERGYRDAVEMAAIAASSLGLEPESVGVAST
                     GVIGRYLDLPRIREQCSRVSPLLARNAAAEDAAARAIMTTDTFPKHALFKTESFTVGG
                     IAKGSGMIAPNMGTMLAFIYTDADVEAGVLLEILRQASRRSFNRVVVDGDTSTNDAAF
                     CTATGAADRVNRDDLARGIEAVCRDLAIQIARDGEGATKLLKVTVRGAPDEEAAAAVA
                     RTIIASPLVKTAVYGEDPNWGRVVAAAGRAGVEFDPYAVSLRIGDVALVCRGEIVAEL
                     EAAKAAMRGDTVAFDLDLAVGDGQATAWGCDLTERYVEINGKYTT"
     gene            complement(4143..4634)
                     /locus_tag="PK196_01335"
     CDS             complement(4143..4634)
                     /locus_tag="PK196_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON40101.1"
                     /translation="MKVKDIMTPNPVTVRVDSPLREAAGLLRKYHIGGLPVMDGDRVA
                     GIITETDILSLLDVGDLSDDLWLPSPLEVIEIPVREFINWERTKRALTDISNMEVRRV
                     MSSPVIAIDEDADISEAASLMLREGIARLPVLREGKLIGIVTRADIVNGLGASAGEEE
                     EEP"
     gene            complement(4648..5634)
                     /gene="argC"
                     /locus_tag="PK196_01340"
     CDS             complement(4648..5634)
                     /gene="argC"
                     /locus_tag="PK196_01340"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HON40102.1"
                     /translation="MEIAIIGASGYTGGELMRLLLNHPSAEVAVATSRRLDGTPVASV
                     HPHLRGLTDLVFRNTEAGDIDADFVFLAVPHTAAMKVAGTLVERGIKTVDLSADYRLP
                     KDVYEKVYGVTHQAYFEAPYGLTELHRDEIRGASFVANPGCFPTGATLAAAPLAGFAH
                     TIIYDSKTGVSGAGNTPSETTHYPNVGDNFSAYKWTTHRHLAEMRQEIARLGSGARCY
                     FTPHLLPVNRGILTTAHILLSKPMEQKEVEALYRRYYADEFFVRYQKPTLAAVRGTNF
                     CDVAVESEGERVVAVSAIDNLVKGASGQAIQNMNLMCGFAEDAGLRFAGMLP"
     gene            complement(5660..6571)
                     /locus_tag="PK196_01345"
     CDS             complement(5660..6571)
                     /locus_tag="PK196_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HON40103.1"
                     /translation="MLDQFKGCLLGAAIGDALGMARETAPPNFQRLHTGYRRAWRGHP
                     NAGLKPGQFTDDTQTMLLVAGMLAESTYSEQEYAAALARMYMNEELRFPDAAVDAACR
                     RILLSGEVSGSNGVYSSTAGCTGIAVPFGLFYDDPIDVAERVVKACSVTHTHPAAHAG
                     AVSVAMLVHHAVRGRPDAITLAGKHATLEDVALGNRIRDALNLEQEGISLEGALSVIG
                     NDVTVYQTIPLAFFLISRIKDVTILLTTAANIGGNADTIAFICGAYAGAVYGRSALPQ
                     DLLEGLEGKDGIESMAARLYQRCTAKP"
     gene            6851..7315
                     /locus_tag="PK196_01350"
     CDS             6851..7315
                     /locus_tag="PK196_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HON40104.1"
                     /translation="MIFWYILSGYLEPFYIGAGIICCAVVALISTDLLFLSDETGILR
                     SLGTFIRFIAYIPRLILDIVLANIDVAYRVLHPRMPIDPGFITLDTPFQNDILRTAFA
                     NAITLTPGTVTVEVAGGRYTVHALVREAAEESLLKERGMQNSLAHVFGEGEE"
     gene            7312..7575
                     /locus_tag="PK196_01355"
     CDS             7312..7575
                     /locus_tag="PK196_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HON40105.1"
                     /translation="MIDLFYASVVVLILTIFLCLYRVVIGPGVENRLVAVNTIGTTTI
                     IILVLIGLIYGRPIFLDIAIVYAMINFIATLAVARYLKEGCVC"
     gene            7563..7877
                     /gene="mnhG"
                     /locus_tag="PK196_01360"
     CDS             7563..7877
                     /gene="mnhG"
                     /locus_tag="PK196_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HON40106.1"
                     /translation="MRLLNTLASIFIIVGLFFMVVGAIGLLRFPDLYTRVHATGKCDT
                     MGEGMMLLGFILYEGASLFAVKMLLLVVFIFITSPTAVHAIMNAAHTCGTEPWKKGDE
                     RR"
     gene            7874..8119
                     /locus_tag="PK196_01365"
     CDS             7874..8119
                     /locus_tag="PK196_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HON40107.1"
                     /translation="MIWALDFALLLFMIICAIAAITVRDLLHATIILAAYSLIMTVLW
                     SEMNAVDVAFTEAAVGAGVTTVLFIAAIARTERRSSD"
     gene            8116..8391
                     /locus_tag="PK196_01370"
     CDS             8116..8391
                     /locus_tag="PK196_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40108.1"
                     /translation="MNVKALLAVLFIGVVLFWAVEDMPAFGDPEAPATCYTGKLYIDL
                     ALQDVGVENIVTAILASYRGFDTLGETVVIFTAGISVALLLRRGGRL"
     gene            8388..8819
                     /locus_tag="PK196_01375"
     CDS             8388..8819
                     /locus_tag="PK196_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HON40109.1"
                     /translation="MKENVVIRTVSRISVPFIQVFALYVIVHGAVGAGGGFQGGVVFA
                     ASFILMFIAFGLSGVEKRLGKKWLMIYSVIGVAIYAGIGFLCLAAGGNYLEYGAVESA
                     LGVAHLHGYQIDAVEMGIGITVMAVMVSIISDMSDRGGESP"
     gene            8819..9193
                     /locus_tag="PK196_01380"
     CDS             8819..9193
                     /locus_tag="PK196_01380"
                     /inference="COORDINATES: protein motif:HMM:NF005621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HON40110.1"
                     /translation="MEAVFEVFLSHYNYWMAIILIMIGFYALIAKGNLVKKLIGLNIF
                     QTAIFLFYISLAEVEGGIAPIYLPEGMEAVYVNPIPHVLILTAIVVGVSVTAVGLALI
                     VRIYEEYGVIDEAGLLKVVKRR"
     gene            9190..10695
                     /locus_tag="PK196_01385"
     CDS             9190..10695
                     /locus_tag="PK196_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HON40111.1"
                     /translation="MIEHLPVILITVPILSALLILLLGWHDRRFCYPITLATILFQFV
                     GGIALLIEVMRQGTIHYHIGGWAPPLGIEFVIDTFNGYILPIILFLAVAAAVYARRSV
                     ENEIEPEKIVSFYVLFQLLVTGLVGMTITGDIFNLYVFLEISSIAAYTLIASTRRPRA
                     CVAALNYLILGSIAGGFVLLGIGNLYVATGTLNMADIARLLPASYGLATVHTAFLLLV
                     IGFSIKVALFPLHLWLPDAYTEAPSAVTVLISTVMAKVSVYAMFRVVFSVFTTAYFVE
                     TVPVTELILLIAAVAIVAGAVLAIAQDDLKRMLAYSSVSQIGYIVFALGVANQVALEG
                     GLLHILGHAVMKGCLFMVAGVIIYRLGTSRFSDLKGVAATMPVTCATFALAGASMIGV
                     PPTIGFASKYYLVMGALEAGYWVYAAVLLAGSLLAVGYIWRFIEVAYFSASSHHEDGG
                     DHCPAPAVREAPLSMLVPMVVLGFLCLLLGIFVEPIMEIVSPAVSLLLGGA"
     gene            10697..12187
                     /locus_tag="PK196_01390"
     CDS             10697..12187
                     /locus_tag="PK196_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HON40112.1"
                     /translation="MVDATGLLPAVTVVTPLLAAVMILVSGRHENIRDGFTVAAAVAM
                     LGSVVAMLPSILAGERIAVSLIPLLPGGDLALRVDAFGMVFALTAAVLWLLNSFYSIG
                     YMRSLKEHAQTRFFFCLAVAIASAIGIAFSANLVTLLVFYEILTIITYPLVVHVETPE
                     AMAAGRKYLAYLLIGGVFLLAAVVLTYILTGTTGFAPGGFLAGSAPPLTLQILFILFM
                     VGFVKAAWMPLHGWLPAAMVAPTPVSAFLHAVAVVTAGVFGIVRIAGWVYGMDLLASL
                     GLGTLLAVIASFTIITASLFALTEDNLKRRLAYSTVSQLSYILLGVSMLSIAGMTGAM
                     AHIPIHAFLKITLFFVAGAVIVAAGRERISDMKGIGRKMPVTAVMFSGAAIGICGLPP
                     FSGLISKVYLAFGAVEGGVPILLLVLVASTVLNAAYFFPIIHTMLVASPDDERSLDSV
                     SEPPLLMLVPIILTTVASVLIFFYPAVPFMDLVEIAIGEITGSVIP"
     gene            12184..13980
                     /locus_tag="PK196_01395"
     CDS             12184..13980
                     /locus_tag="PK196_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HON40113.1"
                     /translation="MTALPPALIYLIGILFLPLLAGRARKAWTLFIGVVGLGLTLGLS
                     PAVPEIVIRVLPGIDTVLLTVDPVRDLAGGIFAISGLLVLVYAVYRDLPLVETIGILA
                     SIAAAMGVVYAGDFITVFVFWEALAVASIAIIWSSRSPGSSAAGYRYILYHVFGGACL
                     LAGIVYTLSASGSAAIGPVADGPGLVFMLIGIGMNAAFIPIHTWLPDAYPKASVVGSV
                     ALSIFTTKAAVFLLAGVGGWGVAVAWMGGAMALYGAVFALMQDDLRRLLGYSIISQVG
                     YMVAGIGVGSVAGVDAGLAHMVNDILFKSLLFMAAGAVILRTGSHRLSEMGGLWRTMP
                     VTTLSALIGGLALAGLPGLNGAVSKGMAIEAAGAVPYLAPVLMVSAVITVIYVFRILY
                     LGFFRPAEGTREGILPRDPPVPMLLAMGATAILCVAIGLFPHLLTDLLPGGTPAHPFA
                     ASHLLESAGIFAVAGVLLALVRPFRRSWGGFETDIDALYIQAGRAVVWFSSHPLVEVT
                     DAIGRGIGRVVAWFQHVSANPAAAIQIAWKTLVLPFVRIFWGRERSAAYEKDLVFLRD
                     NYPEIQTSIWGGSYGIIFVCILAFLYFLFSLT"
     gene            14134..14307
                     /locus_tag="PK196_01400"
     CDS             14134..14307
                     /locus_tag="PK196_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40114.1"
                     /translation="MEEVMRAAGMRTAFTIARGLSYPINSTFARGYPHGGTLTNNTSI
                     CGGCLNPVTCSPF"
     gene            complement(14288..15529)
                     /gene="thiC"
                     /locus_tag="PK196_01405"
     CDS             complement(14288..15529)
                     /gene="thiC"
                     /locus_tag="PK196_01405"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HON40115.1"
                     /translation="MKTQIEEAREGTITPQMASVAAAERIEADDLRQSIADGEAVIMT
                     RGMSSLGIGRGLRTKVNVNLGTSTAIIRPEDEVRKARIAESVGADTITDLSAGGDIPA
                     IRRAIFSATTLPITTVPIYQTAAEIGIDRMTADGILATIQRQAEEGISSVVIHAVEPE
                     TIDAALDGKRVMGIVSKGGSITAAYMLTREAENPFLEHFDEILSILKEHDIVLSLGNT
                     MRSGCIHDEMDEAQKREMRMNSQLAACAMENGVQTIIEGLGGHVRADRIPRYIQRYRE
                     ASPAPLFVAGPLPTDIAVGYDHIAGCVGASIASGAGADYLCYLTPAEHLGLPTPEQVR
                     EGLIAFRIAAHIGDTIKYGLDDADLTLARSRSRLDREGQIAHAIDPETAQDLIPPSGP
                     CTMCGDFCAVAMMERLKKENR"
     gene            15646..16671
                     /locus_tag="PK196_01410"
     CDS             15646..16671
                     /locus_tag="PK196_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HON40116.1"
                     /translation="MKTAGNLPPPARIAMIAAAVVIVLAGIRAATPILAPLLVAVFFA
                     MITAPLMKWLTRRGAPPVIAAAAVVAGLIGLFAAAVAFLGVAFAGFIRSLPQYRESFL
                     EQVTVLADYGIDIGSFTIWDYIDQGFLIQQVAGIARGVGNIAIDAFLVFVGMGFLLIE
                     APRLAGVLERYIGADTSLYRHLAQSGSFLIDYVIVRTKVNLITGVGTGLFLAVLGVDF
                     AVLWGFIAFILSYVPYIGLVIAAIPPTLLALIELGPAGAATVIVVISLIDSAAENLVL
                     PRMAGRELNLSPFVVLFSVVFWGFILGAVGVFLAIPLTIAVKLFLESWEETRWMGELI
                     GNSSAEE"
     gene            17101..17631
                     /locus_tag="PK196_01415"
     CDS             17101..17631
                     /locus_tag="PK196_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40117.1"
                     /translation="MDLETEIEFHFKSKDQLLDAGDPYPLPEQELTEFAESFIVRYVD
                     GHDPRRVAGIAVGLPRGSLSPEEASLVPEAVRRHYTFRLRDLENDRKMSHREGKIRIL
                     IAAINAGIAVLFFYVFIGYFRGFVMTMLAGLVTILNWVTIWDTYEYIVYDYRRELQKY
                     LIYRKLTEIDIRFVEW"
     gene            17689..18303
                     /locus_tag="PK196_01420"
     CDS             17689..18303
                     /locus_tag="PK196_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40118.1"
                     /translation="MKRPVGLYLAYLFQSLIAVNIIIALSLGEYSQVFTGALGLVLTL
                     VPTIITRRLSITLPWQVNLLIAISLYLHIAGEIRGFYMLYYPYYDKVAHFVSGMTVSV
                     LAFVAVLLLDRFGGLNLPRWMIVGFIVISAMAMGAFWEIYEWVFDTFLGTDLQHGLDD
                     TMLDMIFVLIGAIIVAVAGGHYLSRSSKEEIAEKLVVPEESRTG"
     gene            18465..19331
                     /locus_tag="PK196_01425"
     CDS             18465..19331
                     /locus_tag="PK196_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HON40119.1"
                     /translation="MLYYKDVVFEKPIPAEIQRLGLLPADLHFHTRYSDSATRVRDAL
                     KLASHQGIGLAITDHNQIGGVVEARRQGIDLPLIPGIEVSANDGPHILLYFYSVSDLR
                     DFYRRHIERNRRDGPFTAIRLDTQEIMDRREGYACIAAEAHPCGYSFLNRGVERCVMG
                     SCIGGEIFSRLDAFEVICGGMARTHNLKAARLAAAHHLGRTGGTDGHLLHELGGVVTC
                     AEADTVDEFLDAIQNRESVVIGRERPFAEKAIMATAVLPHHLPYTVPIIQSRWEQELP
                     RIRKFVQGRFGR"
     gene            complement(19338..20771)
                     /gene="cls"
                     /locus_tag="PK196_01430"
     CDS             complement(19338..20771)
                     /gene="cls"
                     /locus_tag="PK196_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HON40120.1"
                     /translation="MDLSLVVWVILPLNVIFAIIIVFFERKDPTEAIAWLVLLFLLPP
                     VGFVLYLLLGQNYTRQKMFVVKEHDDRSFLREIFAEQQRRLTDRNHRFATLEAERFQK
                     TISLLLQNNQAYLTEGNRVEIFTEGEDKFEALFRAIREARHHIHLEYFVINNDDLGRA
                     VIHALAERARQGVEVRLLIDTMGSLRGGGLKVAFRELTDAGGKIGAFFPSIYRVNYRN
                     HRKIAIIDGVAGFIGGFNIGDDYLGKGPLGYWRDTALQVVGPAAWALQLRFFLDWNYT
                     TGEGLRLNSHYFPDTGTPGTTPIQIVSSGPDTRWSPIKDGYINLINSARESVYIQTPY
                     FIPDDSVTDALRIAALSGVDVRIMIPCKPDHIFVYWATLSFIGDLLEAGVRAYTYDAG
                     FLHAKTIVVDREAGSVGSANWDVRSFRLNFEANAFFYDAAVGAELAGAFEEDLSVCTE
                     ITLENYRARPIEIRLKESISRLFSPLA"
     gene            20897..21913
                     /locus_tag="PK196_01435"
     CDS             20897..21913
                     /locus_tag="PK196_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40121.1"
                     /translation="MSCPVCGHDCVADASTVLAGLPGRFDPCPDCTGLIYDKRSPPPD
                     IDTAEPCPTCGRRFIDEVFAHIYRVMVDEGDLAAAEPLASVGTPLVHPGFAMRNPPYL
                     PPRSLLLFSRHVSERTARRLVAGVPEIRGVVRTVDGTPGIRDTDSEPVEYTLLAGCDV
                     RADIFQTRAGPIVVYKQQSTLHIEFPRGRNEKILSLERQISRRRPRAFVDACSGAGTL
                     GLAAALAGVECVICNDAWYAAAYWTARNLEVNRGSLGSGEVTFYRSYGDLRRSPVGRE
                     PILVARADGARVYQGDLRSLPTVLPSEIDLTALDLFEKMDAGRVARIARAWRTRVGGG
                     IFIP"
     gene            21926..22236
                     /gene="ffs"
                     /locus_tag="PK196_01440"
     ncRNA           21926..22236
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK196_01440"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            22248..23213
                     /locus_tag="PK196_01445"
     CDS             22248..23213
                     /locus_tag="PK196_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HON40122.1"
                     /translation="MAKVTILGATGNVGLFAAHTISEIPHVSEMLLVGRPGREDFLEG
                     CCHDLSDSFAARGNDVRLSFSTGFFDAKGSDIVVCAAGAPRRPGQDRNDLAFENAKIV
                     ADAAATIGRSSPDTILLVVTNPVDVMTAVALEYSGLQPRQVFGLGTHLDSMRLKSLIA
                     HYFRVHVSEVHTRIIGEHGDSMVPLWSATTIGGIQIGNLPTFSGLPKQEMIETVRTSG
                     EAIIRDKGATVYGPGEAIATLVRTVLGNENRILTVSSYIKSEIHGIGDVCIGVPARIN
                     REGVFPVPIRLQDDEVAGFRESVEKIRRITDEVMERLEVDLQPLK"
     gene            complement(23234..23488)
                     /locus_tag="PK196_01450"
     CDS             complement(23234..23488)
                     /locus_tag="PK196_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HON40123.1"
                     /translation="MGNVALILKIMPESPDVDREALKEAIRAAVTADDIQEEPIGFGL
                     VALKVAVVVPDRAGAPDEVERALQKIEGVGSVDIIESTLV"
     gene            complement(23491..23655)
                     /locus_tag="PK196_01455"
     CDS             complement(23491..23655)
                     /locus_tag="PK196_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HON40124.1"
                     /translation="MTARDRCTSCNATLAEPGSTRFPCPLCGYEINRCHRCREQSIEY
                     VCQKCGFRGP"
     gene            complement(23701..24303)
                     /locus_tag="PK196_01460"
     CDS             complement(23701..24303)
                     /locus_tag="PK196_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HON40125.1"
                     /translation="MTPSAEPLVVKVGGSLFDRVASLLDIFLEAGRPVLIVPGGGMFA
                     DLVRDLGVSGTPAHWMAVSGMEQFGWYIASHGVKPVSSIAPPEGVEVLLPYSVLRETD
                     PLPHTWDVTSDTIAAWVAQRLKTDLLLLKSVDGIQHRGRLLPAVHDPSLACDEVDPLF
                     LPFVFEHGLRARVINGRDDDRIRRALSGKSVIGTLIDPRF"
     gene            24344..26020
                     /locus_tag="PK196_01465"
     CDS             24344..26020
                     /locus_tag="PK196_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HON40126.1"
                     /translation="MRRIVAAIFFLCLLAGTVAGIQIVEFCPDPYQPGDPDEYLVLEG
                     AGLLDGYVISDGEGGYRFPPGTRIDGRLVVARSGAAFEQAHGYLPDFETEERTPAVPD
                     VIPNGNLLMANRADELLLYHGTTLAQQVAWPADVRPREGQIHYLVDGVWDPRPLLIGQ
                     SRFEPAAFEGVAVTAFAAPDCSYEVFTDTVAGAEREILVNVYEFTDSKMAEDLIRARE
                     RGVNVTVLIEGGPVGGISAEGRAVAGALNRSGIPVLSMTTTDAAHAKYRFDHAKYLVV
                     DGDTVLLGSENFKPDGYPEPGLRGNRGWGVRLEHPGLAGYFVEVFLHDVAGGDIVPME
                     GTAAEPGTSWSSSYRVEFASCRADGARVTPVISPDTSFLIHRLIEGAEESIAIEQAYI
                     TNETGTVLNPYLAAAINASRRGVSVRVLLDSAWFNIEDEADNDEMVDVINRIATAEGL
                     PLEARLVDLEANNLVKVHNKGVIVDDRAVLVSSINWNANSPAFNREAGVIIEHPAIAT
                     YYLAVFDDDWNAADEAGAAGINRFDRLKPVLAVCIIAALAMLYLYRSRRT"
     gene            complement(26025..26342)
                     /locus_tag="PK196_01470"
     CDS             complement(26025..26342)
                     /locus_tag="PK196_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40127.1"
                     /translation="MTEPFHCDIMRCDNLVRRLLPAMRAEMVYRLVNERGISQSEVSK
                     RLGVSRAAISQYMNRKRGCNREEFPENLDLVIERWVSAVASGKGGITLCDICRSTDPS
                     ERL"
     gene            complement(26339..26572)
                     /locus_tag="PK196_01475"
     CDS             complement(26339..26572)
                     /locus_tag="PK196_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON40128.1"
                     /translation="MDDINLPPSSQKILHLLENGGALTHKELVHLSSLAPRTVRYALK
                     RLKDNDMIVEKFNFRDARQILYEYKDPQTVSAR"
     gene            26643..27008
                     /locus_tag="PK196_01480"
     CDS             26643..27008
                     /locus_tag="PK196_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40129.1"
                     /translation="MLERQERQALALLVCVVAVVLAAHIILGAGGRSLVAKPYSPEVP
                     DGALVLLEGKIESITKTSTGGHLLLTVNGTRVFLPSNVAAGLDLHENESVSMYGLVQT
                     YRGEREVVVNAVGDIEVLE"
     gene            complement(27009..27227)
                     /locus_tag="PK196_01485"
     CDS             complement(27009..27227)
                     /locus_tag="PK196_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40130.1"
                     /translation="MPGIRIKNGDLPSGAGVEGGRKKRRDESQGSPAGQEEPAQATAK
                     KEKKPQKSGGQPQFEDTGNADIFYTKTK"
     gene            complement(27351..27854)
                     /locus_tag="PK196_01490"
     CDS             complement(27351..27854)
                     /locus_tag="PK196_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HON40131.1"
                     /translation="MVSDEDVDRLVQDLAREAEARGYRLNPDREFARALVRGLLSNRE
                     RYGYISCPCRLASGNREDDLDIICPCDYRDQDLAEYGACYCGLYVTADVEAGVRTAAP
                     VPDRRPSPAERRQKKEERPGTVGAPRDLKYPVWRCRVCGYLCARNEPPDTCPICRVSR
                     DRFERFV"
     gene            complement(27856..28131)
                     /locus_tag="PK196_01495"
     CDS             complement(27856..28131)
                     /locus_tag="PK196_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HON40132.1"
                     /translation="MQYVPGDDHHKVVLYALSTCGWCARTKQLLTDLGVEFSYIYVDL
                     LTGDEQGTVVREVEKWNPRLSFPTIVIDGSKVIIGFREEEIRKAVGA"
     gene            complement(28465..29973)
                     /locus_tag="PK196_01500"
     CDS             complement(28465..29973)
                     /locus_tag="PK196_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HON40133.1"
                     /translation="MKRVGFIVASLLIFFAILAIPVDPAILAPGAKAVAAVTVLMVLF
                     WVTGVIPLEATALLPLVLFPLLGVLSPVKVAESYGNEVIFLFLGGFIIAMSMQRWNLH
                     RRIALHIIRLVGTSPRRLVLGFMVATAFLSMWISNTATAMMMIPIAVAIILTIIPGTE
                     QSLVNLEGKDRDLARCLVLSIPYAASIGGIATIIGTPPNGIFISQLPAIFPEAPTIDF
                     FTWMKFGVPFAALFLIIAWIWLTMVSYRHMETTLPSAKGLIRKELDKLGPLSTGERWT
                     LIVFALTAFAWIFEQTKDIGGFIIPGLDMIFPGIKDSTIAIFGALLLFLLPVDAKKGI
                     FTMDWEWAVKIPWGILLLFGGGIALSNGFIQSGLAATIVNSLTLLHTLPIVILIFVIA
                     LGVSLATEVSSNTAVAAVLMPIMAVMAISVKINPLFLMMTAAVCASLAFMLPVATPPN
                     AVAYGSGYIEAKDLLRSGWALNIIGVALWTLFLFTVVIWAVGIPLDLPAWAL"
BASE COUNT         5583 a         8905 c         9065 g         6566 t
ORIGIN      
        1 tcgatatcag gccgtgccgc ggctgcaacg cctgcgagag actcaaccgc tgcgtccagc
       61 gggactacat ggactacgcc catgacagga taatcgcggc cgactgcatc atccttgcgt
      121 cacccatcta ctgcatgggg cttgccgcgc aggcgaagat cctggtcgac cgcgcccagg
      181 tcttccgctc aaggaagtac gtccttcacc tgccggtcgt cccgcccgaa cggaaaggga
      241 aacgggtggg agtcttcctc tcgaccgccg ggcaaaactg ggagaacgta tttgatgcgg
      301 ccatcccctc ggtgaagtgc ttcttccagg tcgtcgatat accgaacagg gatatcaggt
      361 acctgatgat aaacggtgtc gatgagaagg gcgcgatcga gcgccacccc acggcgagag
      421 acgatgccgg gcgactggcg cgtgaggtcg cggcgcgcct ccgggaggtt cgtgccgcat
      481 gagaagaccc ataaaagtcc tcgggatctc cggcagtccg cggcggcacg gaaacaccga
      541 gacgctgctc gacgccgtgc tcgatggcgc ggcggaggcc gcgggcagca tcgagaaggt
      601 catcctccgg tcgcttgact acgcctcgtg ccgcggctgc aacgcctgcc acaaaacggg
      661 ggtgtgcgtg atcagggaca gcctcacacc tgtatttgat aagatcgctg atgccgatgt
      721 ccttgttctt gcatccccca tctactcgat ggggatcacg gcggagacga aggggctgat
      781 cgaccgcacc cagttcctct gggcccgcaa gtttatcctt aagaacctct actacccggc
      841 caatcagatc cgacgccgta aagggatctt cgtctcgacc gcagggcttg gctgggagaa
      901 cgtctttgac gcggcgttcc cggctgtcac cgcattcttc aacacaaccg ggttcgatta
      961 ctgggacaac atcatcgcaa acgatctcga ccgttacggc gggattgagg ggcatccaac
     1021 cgcccttacg gaggcgcacg agaaagggcg gcgtgtggtc gaactcctcc agaggatgga
     1081 ggaagagagt ctgtccagcg cgccgggaac ctgaaaataa cgcgctttta gccccaataa
     1141 agttcctggg tgatgtcact ccactcaaag gagtgaccag accaggaccc ccagggaagc
     1201 ggccatgagc acggcaaaga ctgccgggtt ccaggccaga accttgcgtc cgtcaaggat
     1261 acttatcggg agcatgttga aggttgcgat catcgcgttg acccggagcc cgaccagacc
     1321 gacgatggcc agtagctcgc cgccgaggag catgagggcc gcgaagggta tgcagaggag
     1381 gaggtttgtg accgggcctg ctgctgatat ccggccgttc tccgtccggg ttgcgttccc
     1441 atagatgtag gttgcccccg gcgctgcaaa gacgataccc acaagcgccg ccataaccac
     1501 ggcgaccagg agcatctggt tgtccttctg aaactctgcc cagtagccgt agcgcatggc
     1561 ggagaacttg tgcgccagtt cgtggaggac gaaggcaaca cctaccgtta taagcgacat
     1621 cacgaagaag aagaccagtt ccgcgacgtt tacacctccc ctgatgtaga tcagggtgaa
     1681 ggctattgat atggcaagcc acgcgatcag gaggtctctt ctctcgcgag gcggtattcg
     1741 ttccagcatc cggtacctcg tttgtcacag catgataata tccttcgcct cgccgatgca
     1801 aggagccgtc aggtttatcc gacccccgcg ttcaatcatt gttgtatgtc ccttgatgcg
     1861 gtcaggaacg agatccgtga gatagacgag aagatcatcg atctgattgc ggagcggcag
     1921 aaccttgcgg cggaggttgc caggatcaag cgtgagatgg gtctgccact ccgtgacgag
     1981 gcgcagagga gggtggtcct cgaccgggtc ttcgcatacg ctgccgagag ccggatagac
     2041 cccctcgccg tccgcagggt ctttgagacc ctggtcgaga tgagcgagga gcggcagcgg
     2101 gagtgcagcg gcgacggcaa cctgccctga ttaaaagaag tcagggggtg atcatcgtcc
     2161 cgacgccctc gtcggtgaag agttcgagga gcaggttgtg ctcccggttg ccgttgacaa
     2221 cgtgggcgct cgtgacaccg ttcctgaccg ctttcatgca cccctcgagt ttcgggatca
     2281 tcccgccggc gatgacccct ctctcgatca tcccttccgc atcggcgagt gatagacggt
     2341 ggtagacctt cttccggtcg gcatccataa ccccatcgac gtcggtcagg ttgaccagtt
     2401 tgtaagcctt gagtgcaatc gcgatctctg ccgccgcggt gtcggcgttg atgttcaggc
     2461 tccgcccctg gcggtcgatg gcgatcggcg ccactaccgg gatgtagttc tgggcgagga
     2521 gacagtggag cacctcgggg tcgacctcct cgatctcgcc gacatgtcca agatcgacct
     2581 cctcgaaggt ttccccgatc tgcaccctct ggggttccat cttcctggcg atgaggaggt
     2641 tgccgtcgtt tcctgagatc ccgaccgcgc gggtgccgca gtttgcgatg agggagacga
     2701 tcccgtcgtt gatctttccc accagcacca tctgggcgat ctccagcgtc tcggggtcag
     2761 tgatccgcag gccgccgacg aacttcggtt ccttgcccat cgccttcatc ttggcggtga
     2821 tctcgggccc cccgccgtgg acaaggacga ccttcatccc cacgtagtgg aggaggacgg
     2881 cgtccctgat caccgtctcc aggatcttct gatcgaccat cgcgtggccg ccgagcttga
     2941 tcacgatcgt cttaccgtag aacttctgga tgtaggggag agcctccatc agcacgtctt
     3001 ctcgtttcat gtcgtatact tcccgttgat ctccacgtac ctctcggtga ggtcgcatcc
     3061 ccacgctgtc gcctgcccat ccccgacggc aaggtcaagg tcgaacgcga cggtatcacc
     3121 gcgcatcgct gctttcgccg cctcaagttc ggcgacgatc tcgccacggc aaacaagggc
     3181 cacgtcccca atccggagcg agaccgcgta tgggtcaaac tccacaccag ccctgcccgc
     3241 tgccgcgacg acccggcccc agttcgggtc ttcgccatag acggcggtct ttactagcgg
     3301 ggaggcgatg attgtccttg caactgccgc tgccgcctcc tcgtcggggg cgccgcggac
     3361 ggtgaccttc aggagtttcg ttgccccttc accgtcgcgg gcgatctgga tggcaaggtc
     3421 gcggcagact gcctcgatgc ccctcgccag gtcgtcgcgg ttcacccggt cagcagcacc
     3481 cgttgcggtg cagaacgccg cgtcgtttgt gcttgtatca ccgtcgacca ccacacggtt
     3541 gaacgatcgg cgggacgcct gccgcaggat ctccaggagc acccccgcct ctacatccgc
     3601 gtcggtgtag atgaacgcaa gcatcgtccc catgttcggg gctatcatcc cgctcccctt
     3661 ggcgataccg ccgacggtaa atgactctgt cttgaagagg gcatgtttcg ggaaggtgtc
     3721 ggtggtcatg atcgcccgcg ctgctgcatc ctcggccgcg gcgttccggg caaggagcgg
     3781 cgatacccga ctgcactgct cccggatgcg ggggagatcc aggtatcgcc cgatcacccc
     3841 ggtgcttgcg acaccgaccg attccggttc aaggccaagc gatgaggctg cgatggcggc
     3901 catctccacc gcgtcgcggt agccccgctc gccggtgtag gcgttagcgc acccgctgtt
     3961 gacgacgatg gcctcgagtg cacccctccg gattcgctcc atcatcaact caaccggggc
     4021 cgctcgaacc aggttggacg tgaatacacc gctcgcggtt ccgctcgcct ggataagagc
     4081 aagcccgaac tttccgtcct tcatcccggc ggcggagaca ccctctaccg cacagatacc
     4141 cttcacggct cttcctcctc ctcgccggcg gacgccccga gcccgttgac gatatccgcc
     4201 ctggtgacga tcccgatcag tttcccctcg cggagcaccg gaaggcgggc gatcccctcg
     4261 cggagcataa gggatgccgc ctcggagata tcggcatcct cgtcgatcgc gataaccggg
     4321 ctgctcatca ctcgccgcac ctccatgttg cttatatcgg tgagcgccct ctttgtcctc
     4381 tcccagttga taaactccct tacagggatc tcgatcacct cgagcggcga gggaagccat
     4441 aggtcatcgg agaggtcgcc aacgtccagg agcgagagga tatccgtctc ggtgatgatc
     4501 cccgccaccc ggtcgccgtc catcacagga agcccgccga tatgatactt acggagcaac
     4561 cccgccgcct cacgaagagg cgagtcgacc cggacggtca cagggttcgg ggtcatgata
     4621 tcttttacct tcaacatcac catcacctca ggggagcatc ccggcgaacc ggagacctgc
     4681 gtcttccgca aacccgcaca taaggttcat gttctggatc gcctgaccgc tcgccccctt
     4741 gaccaggttg tcgatcgccg agaccgcaac aaccctctcc ccctcgctct ccaccgcaac
     4801 gtcgcagaag ttcgtccccc taacggctgc aagtgtcggt ttctggtatc ggacgaagaa
     4861 ctcgtctgca tagtaacgcc tgtagagcgc ctcaacctcc ttctgctcca tcggtttgga
     4921 gaggaggatg tgggcggttg tcaggatgcc ccggttcacc gggaggagat gtggcgtgaa
     4981 gtaacatctt gcgcctgaac cgagcctggc gatctcctgc ctcatctcgg caagatggcg
     5041 gtgggttgtc catttgtagg cgctgaagtt gtcgccgaca ttggggtagt gagtggtctc
     5101 agagggggtg tttcctgcgc cggagacccc ggtctttgag tcgtagatta tggtgtgggc
     5161 aaaccctgca agcggcgccg ccgcaagcgt cgcaccggtc gggaagcagc ccgggtttgc
     5221 gacgaaagag gcgcccctga tctcgtcccg gtggagttct gtcaggccgt agggggcctc
     5281 aaagtaggcc tggtgggtca cgccatagac cttttcatac acgtccttcg ggaggcggta
     5341 gtcggccgag aggtcgacgg tctttattcc tcgttcaacc agcgttccgg caaccttcat
     5401 cgccgcggtg tgggggacgg ccaggaagac aaagtcggca tcgatatcgc cggcttcggt
     5461 gttccggaag acaagatcgg tcagaccacg gaggtggggg tggacggatg cgacgggggt
     5521 gccgtcaagt cgccgggatg ttgcaacggc gacctccgct gacggatggt tcagcagcag
     5581 tcgcatcaac tcaccaccgg tgtagccgga tgcgccgata atcgcaatct ccataagatg
     5641 gagtctcttt tatagaatct taaggctttg cggtgcagcg ctgatagagc cgggcggcca
     5701 tggattcaat cccgtctttt ccctcaagcc cttccaggag atcctgtggg agggcggatc
     5761 tcccgtaaac cgcgccagcg taggcgccgc agatgaatgc gatggtgtcg gcgttccccc
     5821 cgatattggc ggcggtggtg aggagaattg tgacatcttt tatgcggctg atcaggaaaa
     5881 atgcaagcgg gatggtctgg tagacggtta catcgttccc tatcaccgag agtgcaccct
     5941 ccaggctgat cccctcctgt tcaagattca gggcgtcgcg gatcctgttg ccaagtgcga
     6001 cgtcctcaag cgtggcgtgt ttcccggcaa gggtgatggc gtcgggccgg ccacggacag
     6061 cgtggtggac aagcatcgcg acgctgaccg ctccagcgtg tgctgctgga tgggtgtggg
     6121 tgacgctgca ggccttgacc acccgttcgg ccacgtcgat cgggtcgtca tagaagagac
     6181 cgaacgggac ggcgatacct gtgcagccgg cggtgctgga gtagaccccg ttcgatcccg
     6241 agacttcacc tgagaggagg atgcggcggc acgcggcgtc aactgcggcg tcagggaacc
     6301 ggagttcttc gttcatatac atccgggcaa gggctgctgc gtactcctgt tctgagtagg
     6361 tgctctctgc aagcatcccg gccacaagga gcatcgtctg ggtgtcgtcg gtgaactgcc
     6421 ccggtttaag cccggcgttc gggtgtcccc gccaggcgcg gcggtagccc gtgtggaggc
     6481 gctggaagtt gggaggtgct gtctcccgtg ccatgccaag tgcatccccg atggcggccc
     6541 cgagaaggca gcccttaaac tgatcgagca tttgagtaat actctctaat gtccttctta
     6601 aggattgttg tttgccggtt acggatcccg atcgggatct tgagcccccc cgtcgcccaa
     6661 tacgcgcgta tacacccggg ttgagaaatt ctgggagagt taagggttat accggcggat
     6721 caaaaacaca gtttttatac acccaccccc tcaaagtaag ggtattacac tgtgcaagag
     6781 agggtgtgcg ttgtcttcag gtaggaaaag gccgtcggtg tctcggtata ttatcacatt
     6841 tgcggtattg atgatcttct ggtatatcct gtcgggctac ctggagcctt tctacatcgg
     6901 cgccgggatc atatgctgcg ccgtcgtggc ccttatatca actgaccttc tcttcctctc
     6961 cgatgagacc ggcattttgc ggtcgctcgg gacattcatc cggttcatcg cctatattcc
     7021 gcggctgata ctggacattg tcctcgccaa catcgacgtc gcataccgcg tcctccaccc
     7081 ccgtatgccg atcgatccag ggttcatcac actcgatacg ccgtttcaaa acgacatcct
     7141 gcgtacagcg tttgcaaacg cgatcacgct gacgccgggg acggtgaccg tagaggtggc
     7201 tggcgggaga tacacggtgc acgcccttgt ccgggaggcg gcggaagaat ccctcctcaa
     7261 agagaggggc atgcagaaca gccttgcaca cgtatttggt gagggtgaag aatgatcgat
     7321 ctcttctatg ccagtgtggt cgtactcata ttgacgatct ttctctgtct ataccgggtg
     7381 gtcatcggcc ccggcgttga gaaccggctg gttgcggtga acaccatcgg aacaacgacc
     7441 atcatcatcc tggtactcat tggcctcata tacgggcggc cgatcttcct ggacatcgcg
     7501 atcgtctatg cgatgataaa cttcatagcg acgcttgcag tcgccagata cctcaaggag
     7561 ggatgcgtct gctgaacact ctggccagca tcttcatcat tgttggcctc ttcttcatgg
     7621 tcgtcggcgc cataggtctt ctcagatttc ccgatctcta tacccgtgtc cacgcgaccg
     7681 gcaagtgcga cacgatgggg gaggggatga tgctgcttgg cttcatcctc tacgaggggg
     7741 cgagtctgtt tgcggtgaag atgctcctcc tggtggtctt catattcata acctcgccga
     7801 cggcggtgca tgccatcatg aatgccgcac atacctgcgg gactgaaccc tggaagaagg
     7861 gggatgaacg gcgatgatct gggcgcttga ctttgcactc ctcctcttta tgatcatctg
     7921 cgcgatcgcg gcgataaccg tccgcgacct cctccacgca acgatcatcc ttgccgccta
     7981 ctcactgata atgacggtgc tctggtcgga gatgaacgcc gttgacgttg ccttcaccga
     8041 ggcggcggtg ggggcagggg taaccaccgt tcttttcatt gcggccatag cacgaaccga
     8101 aaggagatct tcagattgaa cgtaaaggct ctgcttgcag ttctcttcat aggcgtggtc
     8161 ctcttctggg cggtcgagga tatgccggcg ttcggcgacc cggaggcccc ggccacctgc
     8221 tacaccggga aactctacat tgatctggcc ctccaggatg tcggggttga gaacatcgtc
     8281 acagcaatac ttgcgagtta tcgtgggttc gatacgcttg gagagacagt tgtgatcttc
     8341 actgcaggga tatccgtcgc tctcctgctc aggcggggtg ggcgactgtg aaggagaacg
     8401 tcgtcatccg gacagtatcc aggatttcgg tgccgtttat acaggtcttt gccctctacg
     8461 tcatagtgca tggtgcggtg ggagctggtg gagggttcca ggggggtgtc gtctttgctg
     8521 catcgttcat cctgatgttc atcgctttcg gactctctgg cgtcgagaag cggcttggaa
     8581 agaagtggct tatgatctac tccgtcatcg gggttgctat ctatgcaggt atcggcttcc
     8641 tctgtctcgc cgctggcggg aactacctgg agtacggagc agtcgaatcg gcgctggggg
     8701 tggcccatct ccatggatac cagatcgacg cggtcgagat ggggatcggg atcaccgtga
     8761 tggcggtgat ggtctcgata atctcggata tgtccgatag gggaggtgag tctccgtgat
     8821 ggaagcggtc tttgaggtct tcctctcgca ctacaactac tggatggcga tcatcctgat
     8881 aatgatcggg ttctatgcgt tgattgcaaa agggaacctg gtaaagaaac tcatcgggct
     8941 caacatcttc cagaccgcga tcttcctctt ctacatctcg cttgccgagg tggaaggcgg
     9001 tatagccccg atctacctcc ccgaagggat ggaggccgta tacgtcaacc ccatcccgca
     9061 cgtcctgata ctgaccgcga ttgtcgtcgg tgtgagtgtg accgcggtcg gtcttgcgct
     9121 gatcgtccgg atatacgagg aatatggggt catcgatgaa gccggtctgt taaaggtggt
     9181 gaagaggcga tgatcgaaca ccttcccgtc atcctgataa ccgtccccat actttcggcg
     9241 ctcctgatac tcctgctcgg ctggcatgac cgtcggttct gctacccgat cacgcttgca
     9301 acgatactct tccagttcgt gggcgggatc gccctcctta tcgaggtgat gcggcagggg
     9361 acgatccact accatatcgg gggatgggcg ccaccgctcg ggattgagtt tgtcatcgat
     9421 accttcaacg gctacatcct gccgatcatc ctcttcctgg ccgttgcggc ggcagtatat
     9481 gcgcgacgga gtgtcgagaa tgagatcgaa ccggagaaga tcgtctcgtt ctacgtcctc
     9541 ttccagctcc ttgtgaccgg tcttgttgga atgacgatca caggggacat ctttaacctc
     9601 tacgtcttcc tggagatatc gtccatcgcc gcctacaccc tgatcgcctc caccagacgg
     9661 ccgcgggcct gtgttgcggc cttaaactac ctgattcttg gctcgattgc gggaggtttt
     9721 gtcctgctcg gcatcgggaa cctctacgtt gcgaccggga cgctgaacat ggcagacatt
     9781 gcccgtcttc tccctgcgtc atatgggctt gcaacagttc ataccgcgtt tctcctcctg
     9841 gtcattgggt tctccataaa ggtcgcactc ttcccgctcc atctctggct ccctgatgcc
     9901 tacacggaag ccccgtccgc tgtgaccgtc ctgatctcga ccgtgatggc caaggtgagc
     9961 gtctatgcga tgttccgggt tgtattctcg gtcttcacga cggcatactt cgttgagacc
    10021 gtccctgtga ccgaactcat cctcctcata gcagcggtcg cgatagtcgc cggagcggtg
    10081 ctggccatcg cacaggatga cctcaagcgg atgctcgcat actcgagcgt cagccagatc
    10141 ggctacatcg tctttgcgct cggcgttgca aaccaggtcg cgctggaagg cggtctcctg
    10201 catatcctcg gccatgccgt gatgaaaggc tgcctcttca tggtcgcggg ggtgatcata
    10261 taccggctgg ggacgtccag gttctccgac ctcaaaggcg ttgcagcaac gatgccggtc
    10321 acctgtgcga cgttcgccct tgccggggcg tcgatgatcg gcgtcccccc aacaatcggg
    10381 tttgcaagca agtattatct ggtcatggga gcgcttgaag cgggttactg ggtctatgcg
    10441 gccgtcctcc tggccgggtc gctcctcgcg gtcggctaca tctggcggtt catcgaggtc
    10501 gcttacttca gcgcctcgag ccaccacgag gacggtggcg atcactgccc ggcgcctgcg
    10561 gtgcgcgagg cgcccctctc gatgctcgtc ccgatggtcg tcctgggttt cctctgtctg
    10621 ctgctcggca tcttcgtcga acccattatg gagatagtct ctcctgccgt atcgctgctg
    10681 ctcgggggtg cctgagatgg tggatgcaac cggtctgctc ccggcggtaa ccgtggtcac
    10741 ccccctcctg gcggcggtga tgatcctggt cagtggacgg cacgagaaca tacgggacgg
    10801 gttcacggtg gcggccgcgg tggcgatgct tggctctgtc gtcgccatgc tcccctcgat
    10861 cctggccggg gagaggatcg cggtctcgct catcccgctc ctgcccggtg gggatctggc
    10921 gctgcgggtg gacgctttcg gcatggtctt tgccctcacc gccgccgttc tctggctgct
    10981 gaactccttc tactccattg gctacatgcg ttcgcttaag gagcacgcac agacaaggtt
    11041 cttcttctgc cttgcagttg cgatagcctc cgcaatcggg atagcgttct cggcgaacct
    11101 ggtgacgctt ctcgtcttct acgagatcct gactatcatc acctatcctc tcgttgtcca
    11161 tgtcgagact ccggaggcga tggcagcggg gaggaagtac ctggcctacc tccttatcgg
    11221 gggtgtcttc ctgctggcgg cggtcgtcct gacctatatc ctcaccggga caaccgggtt
    11281 tgccccgggt gggttccttg ccgggtcggc accgcctctc accctccaga tactcttcat
    11341 cctcttcatg gtgggcttcg tgaaggcggc ctggatgcct ctccacggct ggcttcctgc
    11401 ggcgatggtc gccccgacac cggtgagcgc gttcctccac gctgtagccg tcgtcacggc
    11461 gggggtcttt gggatcgtca ggatcgcggg gtgggtctac gggatggatc tcctggcctc
    11521 gctcggtctc gggacgctcc ttgccgttat agcctcgttt actatcataa ccgcgtccct
    11581 cttcgccctg accgaggata acctcaagcg gaggcttgcc tactcgacgg tgagccagct
    11641 gtcatacata cttctcggcg tctccatgct ctccatcgcc gggatgaccg gagcgatggc
    11701 gcacatcccc atccacgcct tcctcaagat cacgctcttc tttgttgcgg gagcggtgat
    11761 tgtcgctgca gggagagagc ggatctccga tatgaagggt atcggcagga agatgccggt
    11821 tacggccgtg atgttctctg gtgctgcgat cgggatctgt ggtctgccgc cgttctcagg
    11881 gctcatcagc aaggtatacc tggcgttcgg ggcggttgaa ggcggtgtgc cgattctgct
    11941 gctggtcctg gtcgcaagca cggtcttaaa cgccgcttac ttcttcccga taatccatac
    12001 gatgctggtc gcctcgccgg atgacgagcg ctcgcttgat tccgtcagcg aaccgcctct
    12061 cctgatgctc gtcccgatca tcctgacaac ggtggcatcg gtgttgatct tcttctatcc
    12121 cgccgtgccg tttatggatc tcgttgagat cgctattgga gagatcacgg gatcggtgat
    12181 accatgaccg cgttgccgcc agccttaatc tacctgatcg ggatcctctt cctccccctc
    12241 ctcgccggca gggccaggaa ggcatggacg ctgtttatag gggttgtcgg gcttggtcta
    12301 acacttggac tctcgcccgc ggtgccggag attgtgatcc gggttctgcc ggggatcgat
    12361 acggttctcc tgacggttga ccccgtacgg gacctggcgg gcggtatctt cgccatctcg
    12421 ggtctccttg tcctggtcta cgcggtatac cgggacctcc cgctcgtgga gacgatcggt
    12481 atcctggcct cgattgcagc ggcgatgggg gtggtctacg ccggggactt catcaccgtc
    12541 ttcgtattct gggaggcgct tgccgttgca tcgattgcaa tcatctggtc atcgagatcg
    12601 ccaggctctt ccgctgccgg ttaccgctac atcctctacc acgtctttgg cggggcgtgc
    12661 cttctggccg ggatcgtcta cacgctgtcc gccagcggga gcgctgccat cggtccggtg
    12721 gcggatggcc ccggtctggt cttcatgctg atcgggatcg gcatgaacgc ggcgttcatc
    12781 cccattcata cctggcttcc cgacgcgtac ccaaaggcat cggttgtcgg atcggtcgct
    12841 ctctccattt tcacgacgaa agccgcggtc ttcctgcttg ccggagtggg tggatggggt
    12901 gttgcggtcg cctggatggg cggggcgatg gcgctctacg gtgccgtgtt cgcgctcatg
    12961 caggacgatc tccgccggct cctgggctac tcgatcatca gccaggtggg ttatatggtc
    13021 gcgggcatcg gtgtggggtc ggtggccggg gtggacgccg ggcttgccca catggtcaac
    13081 gacatactct tcaagtcgct gctgttcatg gcggcgggcg cggtcatact cagaaccggt
    13141 tcgcacaggc tctcggagat gggcgggctc tggcggacga tgccggtgac cactctctcc
    13201 gctctcatcg gcggccttgc tctcgcgggc ctcccggggc tgaacggtgc ggtcagcaag
    13261 ggtatggcaa tcgaggcggc aggagcggtg ccctatcttg caccagtcct aatggtatcg
    13321 gccgtaataa ccgtaatcta cgtcttccgg atcctatacc tcgggttctt ccgcccggcc
    13381 gaaggtacgc gggagggtat cctcccgagg gatccgccgg tcccgatgct gctggcgatg
    13441 ggggcaaccg ccatcctctg tgttgcaata gggctattcc cgcacctcct gacggacctc
    13501 ctccccggcg ggacacccgc tcaccccttc gccgcctcgc acctgcttga gtctgcgggc
    13561 atatttgctg ttgcaggggt gctcctggcc ctcgtgcgtc cgttcaggcg ctcatggggc
    13621 gggttcgaga ccgatataga cgccctctat atccaggcag gccgggcggt cgtgtggttc
    13681 tcctcgcacc cgcttgtgga ggtcacggat gctataggcc gcgggatagg acgtgtggtg
    13741 gcgtggttcc agcacgtctc ggcaaacccg gcggccgcca tccagatcgc gtggaagact
    13801 cttgttctgc cgttcgtgcg gattttttgg ggtcgggaaa ggtcagcagc atacgagaag
    13861 gatcttgtgt tcttgagaga taattacccg gagatccaga cgagcatctg gggcgggagc
    13921 tacgggatca tattcgtctg catccttgcg ttcctctact tcctcttcag tttaacgtga
    13981 tagggggagt ttcctctcat ctcacccctc tctggccgtg cggcggcctc ctcggcggag
    14041 atctaccgtg aggacgagga cgtcgagagg acggacttcg caacccatcc agggtgtcgg
    14101 ggccggaacc ttccgggcta cctcctcgcc ctgatggagg aggtgatgcg ggcggccggg
    14161 atgaggacgg ccttcaccat cgccagaggt ctctcctatc ccatcaactc cacgtttgcc
    14221 agggggtatc cccatggagg gacgctcacg aacaacacca gcatctgcgg cgggtgtctg
    14281 aacccggtca cctgttctcc tttttgagcc gttccatcat ggcaaccgca caaaaatctc
    14341 cgcacatcgt gcatggcccg gatgggggaa tgagatcctg tgcagtctcc ggatcaatgg
    14401 catgggcaat ctgcccctcc cgatcgaggc gtgacctgga acgggcgagg gtgagatctg
    14461 catcgtccag gccgtacttg attgtatcac ctatgtgagc ggcaatcctg aaagcaatca
    14521 gaccctcgcg cacctgttcg ggggttggaa gacccaggtg ctctgcggga gtgaggtaac
    14581 agaggtagtc ggcaccagca ccgcttgcga tgcttgcacc cacgcagccg gcgatatggt
    14641 cgtaaccgac ggctatatcg gtgggaagcg gcccggcgac aaagagcggt gccggtgagg
    14701 cctcacgata gcgttgaatg tagcggggta tccggtcagc cctgacatgc ccgccgagcc
    14761 cttcaatgat tgtctggaca ccattctcca tggcgcaggc ggcaagctgc gagttcatgc
    14821 gcatctccct cttctgggcc tcgtccatct cgtcgtggat gcacccgctc cgcatcgtgt
    14881 tgccgaggga gaggacaatg tcatgttcct ttaggatgct aaggatctca tcaaagtgct
    14941 caagaaacgg gttttcagcc tcccgggtga gcatgtaggc ggcggtgatc gatcctcctt
    15001 ttgagacgat ccccatgacc cgctttccgt ccagggcggc atcgatagtc tctggctcga
    15061 cagcatggat gacgacagag gagatccctt cctctgcctg acgttgtatg gtggcgagga
    15121 tcccatccgc tgtcatccta tctattccga tctcggcggc ggtctgatag atcgggaccg
    15181 tggttatcgg gagggtggtg gcggaaaaga ttgcacgcct gattgccggt atatccccgc
    15241 cagcggagag atcggtgatg gtgtccgcac ccacgctctc ggcgatcctg gcttttctga
    15301 cctcatcctc gggtcgaatt atggcggttg atgttcctag gttgacgttg actttcgtcc
    15361 gcaacccacg accgatgcca agagaagaca tcccccgtgt cataatgact gcttctccat
    15421 cagcgataga ttggcggagg tcatctgcct cgatcctctc ggccgctgca accgatgcca
    15481 tctgaggggt aattgtccct tcccgggcct cttcgatctg ggtcttcatt cgggatatta
    15541 ttgtatctcg ggcctgaaaa atcccgctat agggctattt ctccaacgcc cccctctagg
    15601 tgcgtctgaa aaggtctttc ttcctggagc gcttataccg ttcctgtgaa gactgccgga
    15661 aacctcccac cccccgcccg tatcgcgatg atcgcggcgg ctgttgtcat agtgcttgcg
    15721 ggaatccgtg cggccacccc catccttgcg ccgcttctcg tcgctgtctt ctttgccatg
    15781 atcaccgcgc ctctgatgaa gtggctgacc cggcggggcg ctcctccggt catcgccgcc
    15841 gcggcggtgg tcgccggtct gatcgggctc ttcgctgcgg cggtagcctt tctgggtgtg
    15901 gcgtttgccg ggtttatccg ttccctgccg cagtacaggg agagcttcct cgagcaggtg
    15961 acagtcctcg ccgattacgg tatcgatatc ggcagtttca cgatatggga ctacatcgac
    16021 cagggtttcc tgatccagca ggtggccggg atcgcccgcg gggtcggcaa catcgccatc
    16081 gatgctttcc tggtctttgt cgggatgggt ttcctcctca tcgaggcgcc gcgtcttgcg
    16141 ggtgttctcg agcgatacat tggcgcggat acatctctct accggcacct ggcacagtct
    16201 ggcagtttcc tcattgacta cgtcattgtc aggacaaagg tgaacctcat caccggtgtg
    16261 gggacggggc tattcctggc cgtcctcggg gtcgactttg ccgtgctctg ggggttcatc
    16321 gccttcatcc tgagttacgt cccttacatc ggcctggtca tcgcggcgat ccctccaacc
    16381 cttcttgccc tgatcgaact cgggccggcc ggggcggcga cggtcatcgt cgtcatatcc
    16441 ctcatagact ccgctgccga gaacctggtt cttccccgga tggccgggcg tgaactcaac
    16501 ctctcaccgt tcgtcgtcct cttctcggtt gtcttctggg gtttcatcct gggggcggtc
    16561 ggggtcttcc ttgcgatacc gctgaccatc gcggtgaaac tcttccttga gagctgggag
    16621 gagacccggt ggatggggga gttgatcggg aacagcagcg cggaggagta accaccgttc
    16681 agggcggtgg gggtggtcga tgataggaga gaggttatcc cagtgaccca ttccagaaac
    16741 ctgtttacaa cctggtggca cggctttcat ggggcaatca ccactcactg ccatctccag
    16801 gggtggattc gagggttatt gtcagtgaac aaggtttgag gtgccctctt tttcatggaa
    16861 aaggcggaga atttacagtc cagatcgttt agttataggc tccatccgga tagggtgcgc
    16921 gcgctcaacg cccaatcatg cctggtgggg tgagaagatc tctcctgctg cctcgcaagc
    16981 gcagggtccg gaatcataga tatatccgat agcaacgcag atctcccgtg tagagtttct
    17041 gccgggttca ccggttgtcc ggggtgtggg atgcaggaag tccgtgttat cacgaatgag
    17101 atggatctgg agacagagat agagtttcat ttcaagtcaa aggaccagct cctggacgct
    17161 ggcgatccat accccctccc cgagcaggaa ctcaccgaat ttgcggaatc gttcatcgtc
    17221 agatacgtcg acggccacga cccgaggagg gtcgccggta tcgccgtggg cctcccgcgg
    17281 ggttctctct ccccggaaga ggcatctctc gtcccggagg cggtgcgccg ccactacaca
    17341 ttccggttgc gcgacctgga gaacgacaga aagatgtccc atcgggaagg taagatccgt
    17401 atactcatcg ccgcgatcaa cgccggtatc gccgtcctct tcttctatgt ttttataggc
    17461 tacttccggg gtttcgtcat gaccatgctc gctggccttg ttacaatcct taactgggtg
    17521 acgatctggg acacctacga gtacattgtc tacgactacc ggcgcgagtt gcaaaagtat
    17581 ctcatctacc gtaaactcac agagatcgat atcaggttcg ttgagtggtg attccggttc
    17641 gatccgtcgg cacaatatgt ttacgagacc ctcaccaata ttgatctcat gaagaggccg
    17701 gtcggtcttt acctcgctta tctcttccag tcgcttatag cggtgaacat cattattgcg
    17761 ctctcgctcg gggaatactc acaggtcttc accggtgcac ttgggcttgt tttaacgctg
    17821 gttccaacca tcatcacccg ccgattgagc atcacactcc cctggcaggt caatctcctg
    17881 attgcaatct cgctctacct ccatatcgcc ggcgagatcc gggggttcta catgctctac
    17941 tatccttact acgacaaggt tgcccacttt gtctccggta tgaccgtctc agtgctcgcc
    18001 tttgttgccg tcctgctgct cgaccggttt ggcgggctga acctcccccg ctggatgatc
    18061 gtcgggttca tcgtcatttc cgcgatggcg atgggtgcgt tctgggagat ctacgaatgg
    18121 gtctttgaca ccttcctcgg gacagatctt cagcacgggc ttgatgacac catgctcgat
    18181 atgatctttg tcctgatcgg cgcgattatc gtcgcggttg ccgggggtca ctacctctcc
    18241 cggtcgtcaa aggaagagat tgcagagaaa ttggtggtcc cggaagagtc aaggacgggc
    18301 tgagactctc aggtaaacgt cgtttgcgcg gctgggggtt gtggttttgc atcccggtca
    18361 tcgggtcagc gctctggatg ggatgtggaa tggtcctggc gaccaggttt cggggcaacc
    18421 ttttttcttc atggggacat actgctgatc agacaggaca atccatgctc tattataaag
    18481 atgtcgtctt cgaaaaaccg atacccgcag agatccagcg tctcgggttg ctccctgcag
    18541 acctgcattt ccatacccgt tactccgatt ccgccacccg cgtgcgggat gcgctgaaac
    18601 ttgcgtcaca tcaggggatc gggcttgcga tcaccgacca caaccagata ggtggcgttg
    18661 tcgaggcccg gcgtcagggg atcgatctcc ccctcatccc cgggatcgag gtcagtgcca
    18721 acgacggccc gcacatcctc ctctacttct actcggtatc cgatcttcga gacttctatc
    18781 gacggcacat agagagaaac cgaagagatg gccctttcac cgcgatacgc ctcgatacac
    18841 aggaaatcat ggatcgccgg gagggttacg cctgtattgc tgccgaggcg cacccatgcg
    18901 gctactcttt cctgaaccgg ggggtcgaac gttgtgtgat gggcagttgc atcggcgggg
    18961 agatcttctc ccgcctggat gcatttgagg tgatctgtgg tgggatggcc agaacccata
    19021 acctgaaggc tgcgaggctt gccgccgccc accatctcgg gcggacaggg gggacagacg
    19081 gccatctcct ccatgaactg ggtggggtcg tcacctgtgc cgaggccgat accgtcgatg
    19141 agttccttga cgcaatccag aacagagagt cggttgtcat cggtcgtgaa cgcccgtttg
    19201 ccgagaaagc aataatggcg accgctgtcc tcccacacca tctcccctac actgtcccca
    19261 tcatccagtc tcgctgggaa caggaacttc cacggatccg gaagttcgtc caggggcgtt
    19321 ttggccgttg agatcgctca cgccagcggt gagaagagac gtgagatcga ttccttcagc
    19381 cggatctcaa tggggcgcgc ccggtaattc tccagagtga tctcggtgca gacactgaga
    19441 tcttcctcaa aagctcctgc gagttcggca cctacggccg cgtcgtagaa gaatgcgttt
    19501 gcctcgaagt tcaaccggaa acttcggaca tcccagtttg cactcccgac cgagcccgcc
    19561 tctctatcga cgacgatcgt ctttgcatgg agaaacccgg catcgtaggt gtaggcccgc
    19621 acccccgcct ccaggagatc cccgatgaac gagagggttg cccagtagac gaagatatgg
    19681 tctggtttgc acgggatcat gatcctgacg tcgaccccgg agagcgctgc gatcctgagc
    19741 gcatcggtaa cactatcgtc cggtatgaag tagggcgtct ggatgtatac cgactccctg
    19801 gcggagttga tcaggttgat gtagccgtcc tttatcggac tccatcgggt gtcggggccg
    19861 cttgagacga tctgaatggg tgtcgtgccg ggagtgcccg tatccggaaa gtagtgtgag
    19921 ttcagcctca gcccttcacc ggttgtgtag ttccagtcga ggaagaatcg tagctggagt
    19981 gcccatgcgg ccggccccac gacctggagc gccgtgtcac gccagtaacc aagcgggccc
    20041 ttccccaggt agtcgtctcc tatgttaaac cctccgataa aaccggcaac gccgtcgatg
    20101 atggcaatct ttcggtgatt acggtagttg acccggtaga tcgaggggaa gaaggctccg
    20161 atcttcccac ccgcatccgt cagttccctg aacgcaacct ttaacccgcc tcctctcagt
    20221 gaccccatcg tgtcgatcag gaggcggacc tcaacaccct gtcgcgccct ctctgcaagc
    20281 gcgtgtataa ccgcccgccc cagatcatcg ttgtttatga cgaagtactc caggtggatg
    20341 tggtgacgcg cctcccggat cgccctaaag agcgcctcga acttatcctc cccctctgtg
    20401 aagatctcga cccggtttcc ctccgtcaga taggcctggt tattctggag caggagagat
    20461 atggtttttt ggaacctttc tgcctctagt gtcgcgaacc ggtgattcct gtcggtgagt
    20521 ctccgctgtt gctcagcgaa gatctccctg agaaaagagc gatcatcatg ttccttgaca
    20581 acgaacatct tctgccgtgt atagttctgc cctaagagca ggtagagcac gaaaccgacg
    20641 ggaggcagaa gaaaaaggag aaccagccag gctatggcct cagtcgggtc ctttctctcg
    20701 aagaagacga tgatgatggc gaatatgacg ttgaggggga ggatgaccca taccaccagt
    20761 gagagatcca ccaacacgac cacccgtaag gacaggattc ttctatcttc tatctctccc
    20821 tctgggtgta taactatacc cgcgctgcgg gctcatccct gaaacgccta tatagccgga
    20881 gttccaatgc atcggtatga gttgcccggt ctgtgggcac gactgcgttg cagacgcctc
    20941 gactgttctt gcggggctac cggggcggtt tgacccctgc cccgactgca cggggctcat
    21001 ctacgataag cggagcccgc cgccagatat cgatacggca gaaccctgcc cgacctgcgg
    21061 gcggcggttc atcgatgagg tctttgccca catctaccgt gtgatggtag atgagggcga
    21121 tcttgccgcg gccgaaccgc tcgcttccgt cggaacgccg ctcgtccatc ccgggtttgc
    21181 catgagaaac ccgccttacc tcccgccgcg ttcgctcctc ctcttctctc gccatgtcag
    21241 tgagcggact gccagacgac tggttgcggg ggtcccggag atccgggggg tcgtccggac
    21301 ggtggatggg acgccgggga tcagggacac cgactcggaa ccagtcgagt ataccctgct
    21361 tgcagggtgc gacgtcaggg ccgatatctt ccagacccgg gcgggaccaa tagttgtcta
    21421 caaacagcag tcgacgctgc atatcgagtt tccgcgtggt cgtaacgaga agattctctc
    21481 gcttgagcgc cagatcagcc gccgacgtcc ccgggcgttc gtcgacgcct gttctggtgc
    21541 tggcaccctc ggacttgccg ccgctctcgc aggtgtggag tgcgtcatct gcaacgatgc
    21601 ctggtatgcg gcggcctact ggaccgcccg caacctggag gttaaccgcg ggagtctcgg
    21661 gagcggggaa gtgacgttct accgctctta cggcgacctc cggaggtccc ccgtcgggcg
    21721 ggaacctatc cttgttgcga gggcagacgg ggccagggtc taccaggggg atctgcgatc
    21781 cctcccgacg gtccttccgt ctgagatcga cctgaccgca ctcgatctct tcgagaagat
    21841 ggatgccggc agggttgcgc ggatagcacg ggcgtggcgg acccgggtag gcggaggtat
    21901 ttttatcccc taaaccccat tacgtacggg ggctagcccg ggtggctcgg cgtcacctgt
    21961 tacccgaaac cgccgtaacg cggggggcga agtctgagga aggccgcagc ggtttgcagg
    22021 atccgttgcg tcccgacgga gaaacctcgt cccatgaggt cggcggaaag ggatcaaagc
    22081 cccggagggg gcggcgacct tttgccgcgg gtaccggttc aggcccggaa gggagcagac
    22141 tcaccgtgga cgttcggcgc ccatggggtt gcggggtgga gaagggatgc agtggtgaat
    22201 gcttgcgcgc acggtcgacc gaggacgtgc ccccagcggc gaccttcatg gcaaaagtaa
    22261 caattcttgg ggctacaggg aacgttggtc tatttgcggc ccacaccata tccgagatcc
    22321 cgcatgtcag cgagatgctg cttgtcggga ggcccgggcg tgaagacttc ctggaggggt
    22381 gttgccacga tctgtctgac tcgttcgctg cacggggaaa cgatgtccgt ctctcattca
    22441 gtacaggctt ttttgatgca aaaggctctg atatagtcgt ctgtgcagca ggggcgcctc
    22501 gccgcccggg tcaggacagg aacgaccttg cgttcgagaa cgcaaagatc gttgccgacg
    22561 ctgccgcaac catcggtcga tcatcgccag ataccattct attagtggtt acaaaccccg
    22621 ttgacgtcat gaccgccgtc gccctggagt attcggggct ccagccccgg caggtctttg
    22681 gtctagggac gcatcttgac tccatgcgcc tgaaatcgct gattgcacac tacttccggg
    22741 ttcacgtcag cgaagtgcac acccgtatca tcggggagca tggcgacagc atggtcccgc
    22801 tctggtcggc cacgacgatc ggcggtatcc agataggcaa cctgcccacg ttctccggtc
    22861 tgccgaagca ggagatgatc gagaccgtac ggacaagcgg tgaagcaatc atcagggaca
    22921 agggcgccac agtctacggg cccggggaag caatagcaac ccttgtaagg acggtcctcg
    22981 ggaatgagaa ccgtatcctc accgtctcaa gttacattaa aagcgagatc cacgggatcg
    23041 gcgacgtctg cataggtgtc ccggctcgca taaaccgcga aggtgtcttc ccggtgccta
    23101 tccgcctcca ggacgatgaa gttgccgggt tccgcgagtc ggtagagaag atccgcagga
    23161 tcaccgacga agtgatggag cggcttgagg tagaccttca accgttaaaa tagggttttt
    23221 taggaactcg cactcacaca agtgtggatt cgatgatatc gacgcttccg acgccctcta
    23281 tcttctggag cgctctctcg acctcatcgg gtgctcccgc tctgtcgggg acgacaacgg
    23341 caactttcag cgccacaagc ccgaacccga tcggctcttc ctggatatca tcggcggtca
    23401 cagccgccct gatggcttct ttgagcgctt cgcggtcgac gtctggcgat tctggcatta
    23461 tcttcagtat gagagctacg tttcccattg ttatggcccc cggaacccgc acttctggca
    23521 tacatactct atgctctgct ccctgcacct gtggcaccgg ttgatctcgt agccgcacaa
    23581 ggggcagggg aaccgggtgg aacccggttc ggccagcgtg gcgttgcagg aggtgcatcg
    23641 atctcttgct gtcatcgttg tttcctcgaa tcttatccta ttatgtcgcc gccgcgacag
    23701 ttaaaatctc ggatcaatga gcgtcccgat aacactcttc ccgctgagcg cacgccggat
    23761 gcggtcgtcg tctctgccgt tgatcacccg tgctctcaga ccgtgctcga agacgaatgg
    23821 aaggaatagg gggtctacct catcgcaggc gagggaaggg tcgtggacag cgggcagaag
    23881 cctcccgcgg tgctggatgc catctaccga tttaaggagg agaaggtcgg tcttgagacg
    23941 ttgcgcgacc caggcggcga tggtatcgga ggtgacgtcc caggtgtggg ggagggggtc
    24001 ggtctcgcgg agcacagagt agggaaggag aacctcaacc ccctccggcg gcgcgatgga
    24061 ggatacgggc ttgactccgt gcgaggcgat gtaccagccg aactgctcca tcccggaaac
    24121 cgccatccag tgagcaggcg tcccggagac cccgaggtcg cggacgaggt cggcaaacat
    24181 accgccgccg ggcacgataa gcaccggtct tccagcctcc agaaagatat cgagcagcga
    24241 cgcgacccgg tcaaagaggc tccctccgac cttgaccacc agcggttcag cagacggagt
    24301 catatcactc tgctatttaa cccttgaaga cataattttg cgcatgcgcc ggatcgtcgc
    24361 cgcgatcttc ttcctctgtc tcctcgccgg cacagttgcc ggcatccaga tcgttgagtt
    24421 ctgccccgac ccctaccagc caggtgaccc cgacgagtac ctggtgctgg agggggcggg
    24481 gttgctcgac gggtatgtca tctcggacgg ggaggggggt taccggttcc cgcccggaac
    24541 acggatcgac ggccggctgg ttgtcgcgag gagcggggcg gcgtttgagc aggcgcacgg
    24601 ctacctgcca gattttgaga ccgaggaacg tacaccggca gtccctgatg tgatccctaa
    24661 cggaaacctc ctgatggcaa accgggcgga cgaactcctc ctctaccacg ggacgaccct
    24721 tgcccagcag gtcgcctggc ccgctgatgt ccggccgcgg gaagggcaga tccactacct
    24781 tgtggacggt gtctgggacc cacgtccgct cttaatcggt cagtcgcggt ttgagcccgc
    24841 ggcattcgaa ggcgtggccg tgaccgcgtt tgccgctccc gactgttcat acgaggtctt
    24901 cactgatacg gtcgcaggcg cagaacgcga gatcctggtc aacgtctacg agttcaccga
    24961 cagcaagatg gcagaagacc tcatccgcgc cagggaacgc ggcgttaacg tgaccgttct
    25021 catcgagggc ggccccgtgg gtggaatctc agcggaaggg agagcggtcg ctggtgcgct
    25081 caaccggagc ggtatcccgg ttctctcgat gacaacgacg gatgctgccc acgcgaagta
    25141 ccgttttgac catgccaagt acctggtcgt cgatggggat accgtccttc tcgggagcga
    25201 gaacttcaaa cccgacggct acccggaacc ggggctgcga gggaaccggg gctggggtgt
    25261 aaggcttgag cacccggggc ttgcgggcta cttcgtggag gtcttcctcc atgatgttgc
    25321 tggcggggat atcgtcccga tggagggcac ggcggccgaa ccgggcactt cctggtcgtc
    25381 ctcctacagg gttgagttcg cctcctgccg cgcggatggg gcgcgggtga ccccggtgat
    25441 atcacccgac acgagctttc tcatccacag gctgatcgag ggggcggagg agagcatcgc
    25501 gatcgagcag gcctacatca caaacgagac cgggacagtc ctgaacccct accttgctgc
    25561 cgcgatcaac gcctcgcggc gcggtgtcag cgtcagggtg ctcctggact cagcctggtt
    25621 caacatcgag gatgaggctg acaacgacga gatggtggat gtgatcaacc ggatcgccac
    25681 ggccgaaggg ctcccgctcg aggcgcgact cgtcgatctc gaggcaaaca acctggttaa
    25741 ggtccacaac aagggtgtaa tcgtcgacga ccgggcggtg cttgtcagca gcatcaactg
    25801 gaacgccaac tctccggcgt tcaaccggga agcaggcgtg atcatcgagc atccggccat
    25861 cgccacctac tatctcgcag tctttgacga cgactggaac gccgcggatg aggctggcgc
    25921 ggcggggata aaccgattcg atcgactcaa accggtgctc gcggtctgca taatcgccgc
    25981 gcttgcaatg ctctacctct acaggtcgag acgcacctga agtctcatag ccgctcagag
    26041 ggatcggtgg agcggcagat gtcgcagagg gttatcccgc ccttgcctga ggcaacggcg
    26101 gatacccatc gctcgatgac caggtccagg ttctcgggga actcttccct gttgcatccg
    26161 cgttttcggt tcatatactg ggatatcgct gccctgctga ccccgaggcg ctttgagacc
    26221 tcactctggc tgataccccg ctcgttcacc agacggtata ccatctcggc ccgcatcgcg
    26281 gggagcagtc tcctgacaag gttgtcacac cgcattatgt cgcagtggaa tggttctgtc
    26341 atcgcgcgga caccgtctgg gggtctttgt actcatagag gatctgcctt gcgtctctga
    26401 agttgaactt ctccacgatc atgtcgttat ccttgagcct ttttaatgca taccggacgg
    26461 tgcggggtgc aagagaactg agatggacga gttccttgtg cgtcagggca cccccattct
    26521 caagcaggtg gaggatcttt tgcgaggagg gggggaggtt tatatcatcc atgcaggaca
    26581 gttaacgctg ttaacatatc aatttgacgg aagttatctc cccggacgta gagagtataa
    26641 ccatgcttga gcggcaggag aggcaggcac ttgccctgct ggtatgtgtt gtcgcggtgg
    26701 tgcttgcagc gcacatcatc cttggtgccg gtgggcgatc gctggttgca aaaccctact
    26761 ccccggaggt gccggacggg gcgctggtgc tccttgaagg taagattgag tcgatcacaa
    26821 agacctcgac cggcggtcac ctgctcctca ccgtcaatgg gacacgggtc ttcctccctt
    26881 cgaatgtcgc tgccggtctt gaccttcacg agaatgagag cgtctcaatg tatggtctcg
    26941 tccagacgta tcgcggagaa agggaggtgg tggtgaacgc cgtcggcgat atcgaggtgc
    27001 tcgaatgact actttgtctt cgtatagaat atatcggcgt tcccggtatc ctcgaactgc
    27061 ggttgtcccc cggatttctg cggcttcttc tctttctttg cagtcgcctg cgcgggctcc
    27121 tcctgcccgg ccggggaacc ctggctctca tctcttcgct tttttcgccc cccttcaacg
    27181 ccggctccgg aggggaggtc accgttcttt atccgtatgc ccggcataac tggatagttg
    27241 tcctcccgct atatatatga gacctctgga tccccggcgg tatggatacc ctccgcactg
    27301 ccgtggctgc accgatcgga aaaccgcctg tggtgcagtg ttttgctggt tcacacgaac
    27361 cgctcgaacc ggtcacggga gacccggcag atcggacatg tatcaggcgg ttcgttcctt
    27421 gcgcagaggt agccgcagac cctgcaccgc cagaccgggt atttcaggtc tcggggtgcg
    27481 ccgactgtcc ctgggcgctc ctcctttttc tggcggcgct ctgccggcga tggccggcga
    27541 tccgggacgg gagcggcggt gcgcaccccc gcttccacgt cggcggtgac atacaggccg
    27601 cagtagcagg cgccgtattc ggcgagatcc tggtcacggt agtcgcaggg gcagatgata
    27661 tcgaggtcat cttccctgtt tcctgaagcg agccggcatg ggcaggagat gtagccgtag
    27721 cgctcacggt ttgaaagcag gccgcggacg agtgccctgg caaactcccg gtcagggttt
    27781 agacggtagc ccctggcctc cgcctcacgc gccaggtcct ggacgagccg gtcaacatcc
    27841 tcatcgctta ccatctcaag cacccaccgc cttgcggatc tcctcttccc tgaaccctat
    27901 aatcaccttt gatccatcga tgacgattgt cgggaacgag agccgggggt tccacttttc
    27961 gacctccctt accacggtgc cctgttcatc gccggtgagc aggtcaacat agatgtagga
    28021 gaactccaca cccaggtcgg tgagaagttg ttttgttcgg gcgcaccatc cacaggtgct
    28081 cagggcgtag agcaccacct tgtggtggtc gtcaccgggc acgtactgca tctctaccac
    28141 acggtttcct ccattggatt cagagttacg atcagcaggg gtaagtaact taccatccgt
    28201 ccgcgccatc ccaggccatc aaaccggttc attcaggctc tctccaggct cgatgaaccc
    28261 cggcacggat ggaggtgtgg gtgtccatcg tcagggggat agggtatccg ggtgagacgc
    28321 ggagaggcgg cagaggccgc ggccataccc gggatgaccc cggcagccat aacaccccac
    28381 tgccgctctc aagcgcttct tgcagcagga atcactcttc tcccgacgcg gtgcgccggt
    28441 aaaaaataga ggttacctgg gggttcagag cgcccaggcg ggaaggtcga gcgggatacc
    28501 gaccgcccag atgacgacgg tgaagagaaa gagtgtccat agtgcaacac caattatatt
    28561 aagcgcccag cccgatcgga gaagatcttt tgcttcgatg tagccgcttc cgtatgcgac
    28621 ggcgtttggc ggggtggcga cggggagcat gaatgccagc gaggcgcaga cagcggcggt
    28681 catcatcagg aagagcgggt tgatcttcac gctgatggcc atgaccgcca tgatcggcat
    28741 cagcacagcg gccacggcgg tgttcgatga gacttctgtt gcaagcgaga cgccaagcgc
    28801 tatcacaaag atcaggatga cgatcgggag cgtgtggagg agggtgagag aattgacgat
    28861 cgtcgcggca agcccgctct ggatgaagcc gtttgatagg gcgatacccc cgccgaagag
    28921 gaggaggatc ccccagggga tcttcaccgc ccactcccag tccatggtga atataccctt
    28981 ctttgcatct accgggagga gaaagaggag aagcgccccg aatattgcga tcgtggagtc
    29041 ctttatcccc gggaagatca tgtccaggcc cgggatgatg aatcccccga tatcttttgt
    29101 ctgctcaaat atccaggcga acgcggtcag ggcaaagacg ataagcgtcc atcgctcgcc
    29161 cgttgacagg gggccgagtt tatcgagttc cttgcggatg agccccttcg cgctcggcag
    29221 cgtcgtctcc atatgtctgt aggagaccat tgtgagccat atccaggcga ttatgaggaa
    29281 taaggcggcg aacgggacac cgaacttcat ccaggtgaag aaatcgatgg ttggtgcttc
    29341 gggaaagatc gctggaagct gggagatgaa gatcccgttt gggggtgtgc cgatgatcgt
    29401 tgcaatgccg ccgatgctcg cggcgtaggg tatggagagg acgaggcagc gtgcaagatc
    29461 ccgatccttc ccttccaggt ttacaagaga ctgctcagtc cccggtatga tcgtcagtat
    29521 gatcgcgacc gcgatcggga tcatcatcat cgcggtggcc gtgtttgaga tccacatcga
    29581 gaggaaggcg gtggcgacca tgaacccgag gacgaggcgt cttgggctag tcccgacgag
    29641 acggattatg tgcagggcga tacggcggtg gaggttccat cgctgcatcg acatggcgat
    29701 gatgaacccg ccgaggaaga ggaatatcac ctcgttgccg tatgattcag caaccttcac
    29761 cggcgaaaga acgccaagca gcgggaagag gaccagcggc aggagggcgg tcgcctcaag
    29821 aggtattaca cccgtgaccc agaagagaac cataaggacg gtcacggccg caacggcctt
    29881 tgcccctggt gcgaggattg caggatcgac gggaatcgcc agaatcgcga agaaaatgag
    29941 gagtgatgct acgatgaagc cgacccgttt cattgctaga aatgggtgag ggaattattt
    30001 aagtgtagat ggatcaaatt gggcgtcaaa ggggatgtaa atataaaatt cacgtctttt
    30061 ctatagattc tgttgttgcg gctgcctctt catgttgccg aggcggccga acccggagg
//
LOCUS       DAOSZX010000008        29121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1327_length_29121_cov_20.134040, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000008 DAOSZX010000000
VERSION     DAOSZX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29121
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_29121_cov_20.134040"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..694
                     /locus_tag="PK196_01505"
     CDS             161..694
                     /locus_tag="PK196_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HON40134.1"
                     /translation="MLLEAAQAADGTTEPYQSAYAITQYVNGAMNYTYEDPYLEYIFS
                     DLYMMDHPSPINGKYQGVCDEYGTLYTAFARALGIPTRFLSFTMRLPSGNITGHAIAE
                     SWNGTTWVHSDPPWNVFDNPQVYRAAGNDHINITVYGDADDSHYTQDPNDPTGDGILR
                     YEDFRTQILLGEVPRYN"
     gene            705..1565
                     /locus_tag="PK196_01510"
     CDS             705..1565
                     /locus_tag="PK196_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40135.1"
                     /translation="MPKSIVLLALGLTVAVLACSIPVGAVVTPLPGQGNQTGILFGVH
                     PNAKTNETIIAFIAEHGEIWWHEGTYNHTHEYVYVVPANACEEQLVYLNRIRADEHGG
                     EQSIEQIWIIGCDEAMPLAAELRFPEIFAEYPALSNDEGLRSYVENQSPNRYEEITSK
                     PARAQVYFINDTGVFTEYIVDLSGGEIASTTYVSEENLTVGRAEAEAQALSGRSPDAR
                     VENTMLKVYSGDQIVWEITVRAGQRTDVVQVPSDLAPGQSLRTTTPGFGAVLAVLGAG
                     MGFVLYRRRR"
     gene            complement(1662..3005)
                     /gene="gltA"
                     /locus_tag="PK196_01515"
     CDS             complement(1662..3005)
                     /gene="gltA"
                     /locus_tag="PK196_01515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HON40136.1"
                     /translation="MGERPAEIRIHDFEEVDLGLSPDEAVAEAERCLQCKKPLCVKGC
                     PVGIDIPGFVHQVAEGNFPAAARSLKDQNMLPAICGRVCPQETQCEGVCVLGNKETPI
                     RIGALERFVADWERQNNPTLPDVARPTGKRVAVVGSGPAGLTAAAELARAGHAVTIFE
                     SLHAAGGVLAYGIPAFRLPKDIVRAEIEQVLALGVRLKKNHLVGMSVGLDELLGYDAV
                     FLATGAGLPAFMGIPGENLNGVYSANEFLTRVNLMHADAFPEYDTPVLHAGRVAVIGG
                     GNVAMDAARVARRLGAKVYLIYRRGEEEMPARRAEVVHAKEEGIEFLTCTNPIRILGK
                     GGVTGIECVKMSLCEMDASGRRSPEPIEGSEFTLDVDMVIQAIGTSPNPLLVSLIPGL
                     ERGRKGNVVVDKNGRTSIPHVYAGGDIATGAATVIEAMGSAKRAAAAINAMLKGE"
     gene            complement(2998..3858)
                     /locus_tag="PK196_01520"
     CDS             complement(2998..3858)
                     /locus_tag="PK196_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HON40137.1"
                     /translation="MKRLYRIESAARLADRVYEYWIHAPHVARHARAGQFLIVRLHEK
                     GERIPLTISAARGDAVRVIFMTVGKTTEELATLQAGESIMDVAGPLGRPSEIDNYGTC
                     CVIGGGVGIASTPPIAAELKRAGNHVIGIIGARSADLLILEDEMREICDELYITTDDG
                     SRGVHGFASDVLKRLFEERTIDRVWIIGPAIMMKVTSAVTAPAGVKTYVSLNPIMVDG
                     TGMCGSCRVSVGGETKFACVDGPEFDAHQVDFDNLMQRQRIYTAQEKTALERFAEHRC
                     RCGEGGEQHG"
     gene            4327..4503
                     /locus_tag="PK196_01525"
     CDS             4327..4503
                     /locus_tag="PK196_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40138.1"
                     /translation="MQSQIIWGLALTLLGGVVAGAAIAWLPVLLIYAVPLIAMGVALI
                     IWRGREEILEPVEE"
     gene            4510..4833
                     /locus_tag="PK196_01530"
     CDS             4510..4833
                     /locus_tag="PK196_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HON40139.1"
                     /translation="MILTTTAEVPGHEIEEILGVVFGNTVRTKNIGKDITAGLKSIIG
                     GELEEYTDMLTDARTEAYNRMVNFARELGADAVVNIRFTTSQTMSTAAELLAYGTAVR
                     LVPKK"
     gene            complement(4854..6482)
                     /gene="arcS"
                     /locus_tag="PK196_01535"
     CDS             complement(4854..6482)
                     /gene="arcS"
                     /locus_tag="PK196_01535"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HON40140.1"
                     /translation="MSRYEARCRDGLARASAYEHGGKTVFLPAALDTDALFPSLRDRP
                     LSNVPLAADQAFVERYFSPGDGEPVAVHPLSASAESGDCVLAANWQAALSSPRNYAAW
                     LASLKERLPPDTAWYAPAAALPSTVCLLVYSGFDIFDYRGVDLASAQKRFCLPEGEFP
                     AGLMDGGVCGCEGCRAGDLRRHNRLALDREIALVRHHIETGRLRELVEERCRANAAEV
                     AALRFLDRNYAFMERRLPVARAAPMLAATAESQNRAEIRRFADRVIERFAPTRTDVAV
                     LLPCSAKKPYSLSRSHKLFMNAVNRRAHELIVTSPLGLVPRELERVYPAAHYDVPVTG
                     YWDREECAFIADILARYFAAHPYRRVIAHLDGGALSVAEMAAKACGIDLEVTCRGHPA
                     SPESLRALDEALAGERRVQTDTIKGTASWQFGTEINTKGLQVRGRSMQMAVLRGREQI
                     FSIDEGTGLLRPTFAGWDLIPEGYRVRIDEFVPQGDVLAPGVVDCDPRIREGDEVLVE
                     GPLAVATGRAMMGADEMLRSKRGVAVRVRKVKKT"
     gene            complement(6469..7920)
                     /gene="tgtA"
                     /locus_tag="PK196_01540"
     CDS             complement(6469..7920)
                     /gene="tgtA"
                     /locus_tag="PK196_01540"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HON40141.1"
                     /translation="MTITFEVIHKDIAGRVGRLRVNEKTVRTPALLPVVNPHLPLVTP
                     REMGRMGIEALITNAYIFRRSADYRDRALAEGLHRVLDFDGAIMTDSGSFQLSVYGEV
                     EVDNRETLEFQQAIGSDIIVPLDIPTPPDAGRQRAEQDLGVTMERIREAHRLFPDANL
                     AGPVQGGIFADLREESGRAVRELGLTFCPIGAVVPLMEAYRYRDLVRVVLAAKRGLSP
                     AIAVHLFGAGHPSMFALAVAMGCDLFDSAAYALFAREGRYITTHGSLKIDELAELPCP
                     CQVCRSMTADELRKSEDRERLLALHNLHVTLAEISRIRQAIQDGTLWELVDERCRSHP
                     RLLDGYRELLGHTAELEVDDPVSKRRFFYGGAETCRRTEVLRYHRVIPRIPLGGRVLV
                     SFDGRGAPGYDTVLNFKPPFGPYPPELAETFPIGQSETPEWDEEMVRAGCAGIRSLVE
                     ANPGKEFAVRCADEWARLVREEVPGAEVLHEQV"
     gene            complement(7926..8492)
                     /locus_tag="PK196_01545"
     CDS             complement(7926..8492)
                     /locus_tag="PK196_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HON40142.1"
                     /translation="MEMLTPEEGRMAVRLARMAIERAVNGERMVLPPLPPVFGEKRGV
                     FVTIKRHGLLRGCIGLPYPIKPLGEAIIEAAASAALEDPRFPPVSSLEVDDIEIEVTV
                     LTPPRPLECPPLERPEHVEVGRHGLIVSGLGRAGLLLPQVATEYGWNSREFLDQTCVK
                     AGLPPGCWRRGDVAVLTFEGQVIEEKAV"
     gene            8797..9143
                     /gene="rnpB"
                     /locus_tag="PK196_01550"
     ncRNA           8797..9143
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK196_01550"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(9343..9415)
                     /locus_tag="PK196_01555"
     tRNA            complement(9343..9415)
                     /locus_tag="PK196_01555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9380..9382),aa:Lys,seq:ctt)
     gene            9533..10996
                     /locus_tag="PK196_01560"
     CDS             9533..10996
                     /locus_tag="PK196_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HON40143.1"
                     /translation="MPEAVQNVSKERVKYIILGCGSTGYNVAEELAKETEELIIVDKD
                     EKRVEDLRDQKYNALVRDFRNPGFMDGLPVPEVAFILANDREANLAALKTIKSRYPAT
                     YVIARAIDAVSVDLLQQEGADVVLYPQEVVARTAIYHIRKLHSSRLALRLYDLLASWE
                     GTLCIVTHLNPDPDSISSAMALSLIARHASHNKLNCRIVYEGDIGHQENRAFINLLDI
                     KMERLTPDMLAECNYIALVDSSGPGVNNNLPKSTRVNIIIDHHKNNTEPPSTGADFID
                     IRPGVGATASIMTQYLMELDIPVSKTVATALLYGIRADTRDFKRNVTPQDLNYAAFLL
                     PLTDADLLDKITSPSVSLETIEIIGNAIRNRKINSGYLFSNVGYIRNRDALPQAADML
                     IHLEGVNTALVYGITDQNIVISARNKDIRLHLGNVMAEAFGSIGEAGGHATMAAAMIP
                     LSYFSMAKDKEGLLALIIDPILKRFSEIVGLDGEGEE"
     gene            10993..11616
                     /locus_tag="PK196_01565"
     CDS             10993..11616
                     /locus_tag="PK196_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HON40144.1"
                     /translation="MRFEDWEPHYTAILEYFGFEREADEEAARVLADLAGGRDDIALL
                     EALIRGRNVTVCGNAPSLPAELDRIEGAVLAADAAAEVLAGHGVRPDAVFTDLDGATD
                     LFIDLSRQGTVMVVHAHGDNIPLLRHWVPRIPGPLVATTQAAPIPGVHNFGGFTDGDR
                     AVFAASDLGAADVRIIGFDLADRDVDPLKRGKLYWAGELLRMMGYDL"
     gene            11606..13252
                     /locus_tag="PK196_01570"
     CDS             11606..13252
                     /locus_tag="PK196_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40145.1"
                     /translation="MTSEALIIDGYVDEPACLGVPPYISPYIREVAGVLAAHGYAPRY
                     ATIDQVRANPSLLAGHGRGDLVVMIAGLTVPGAYIGGRPATLTEIQQIGLTLREPATA
                     IGGPINFGYAPGGGRKAIRQAIAGFDAVLRGSPAVALDSWLSGGEPEGERDYALSDPW
                     CAAGAGIIAQHPSFPHVICELETATGCPRATVGGCSFCTEPFYGFPRYRSIDGIADEV
                     AALHAAGARHFRLGRQPDLLSYRSTGGGEFPLPRPEALEELFSAVRENAPGLKTLHID
                     NINPGTIARHPDAARAALEVIVAGHTPGDTAAFGMETADPAVVRANNLKAMPEDVFRA
                     IEVVNEVGGRRTRGIPELLPGLNFIIGLAGETPATFDANEAFLRRVLDAGLLVRRVNI
                     RQLMPFEGTAAYEENTLGKHDARFRAFKDWVRLEFDEPMLRRVFPVGSILKEVVIEVS
                     GRPSFGRQMGSYPILVGIPLELPVRTVIDAVVVDWGSRSVTALPYPVEINTLPPAALR
                     WIPGIGRKRAASIAARRPFSSLEEFRAVVGETGLDDLIMF"
     gene            complement(13357..14490)
                     /locus_tag="PK196_01575"
     CDS             complement(13357..14490)
                     /locus_tag="PK196_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON40146.1"
                     /translation="METSLQIGNIAGIPVKIHWSFLLVIPLFAWIIGSQIVLTTELIR
                     ILFGVPIDATLITAGLNPYILGTVVALGLFFGVFVHEVAHSLIAKAKGIKIHGITLLI
                     LGGVSQMEEVVPDPKVELPMALAGPLTSLAIGIICSALVYVFPVLPDPAVAGVLIFTF
                     GYLGILNVLLFAFNLLPAFPMDGGRVLRAWLARRMPLSRATQIAADVGKGFAVLFGIF
                     GFLMLNPILILIAFFIYIGANQEATFLRYYVLLQDVTVADAMSNSLVTVEPETPLPRL
                     LELMYETKHLGFPVVERGNLVGIVTLSDIHKVSPIDREAMQVRDVMTRDPITLPPSAP
                     LIDALRIMTTHGIGRIPVVDDGDLVGIVTRTDVLRVMELREKA"
     gene            14542..14811
                     /locus_tag="PK196_01580"
     CDS             14542..14811
                     /locus_tag="PK196_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HON40147.1"
                     /translation="MRTQVTEIFGLPVYTDKAVFIGNVDDVVLDVDQKKIAALAIGDL
                     NPEIGEVKGYTGLQIPFRIVKSVGDVVIVRHIPGLFRSPAKEEQP"
     gene            14846..15580
                     /locus_tag="PK196_01585"
     CDS             14846..15580
                     /locus_tag="PK196_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HON40148.1"
                     /translation="MDIRDREIFSNLTIFPPVFVRLDGRAFHRVARALNLKKPFDPSF
                     SASMRAVCRYLLTGSGLSPVFAYTFSDEISLYFKTLPFSGRVEKLDSVIAAAAASKLT
                     IEVGCTRPLAFDARTIPAAGEFAVEYLVSRQNEAWRNHINAYCQSALMEEGMTSRQAA
                     AALRGMRSEEMHEMMFERGINLAATPAWQRRGTLLYREECTKEGYNPVTGETVQVMRT
                     CIRELEETPLFSNPEGAALIRSLTGA"
     gene            complement(15563..18730)
                     /gene="ileS"
                     /locus_tag="PK196_01590"
     CDS             complement(15563..18730)
                     /gene="ileS"
                     /locus_tag="PK196_01590"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HON40149.1"
                     /translation="MKEVTASYNPQEIEAGVQDRWRRENIYPRVQEMRKDGDAFFFVD
                     GPPYTTGNIHLGTAWNKIIKDAILRYHRMLGRKIIERAGYDMHGLPIEVKVEHQLGFT
                     SKKDIEDYGIAQFIEQCRTFAVTHMEVMSEQFRRLGVWLDFDDPYQTIKEEYIESAWW
                     TIQRAEERGLLERGYRVVNWCPRCETAIADSEVEYWDETDPSIFVKFPITGRDKEYLV
                     IWTTTPWTLPANVAVAVNPAFTYARVAAKKDGAEEILWIADELVESVLKLGRYQDYSV
                     LERHSGTELIGTEYTSPLARDVPRQAEIQHRIVAADFVELENTGLVHIAPGHGWDDYL
                     IGIREGLEVFCPVDTGGCFTPEAGIFADMYVRDANDIIIEALGSSLLARKSITHRYGH
                     CWRCKTPIIYRATAQWFLKVTEIRDTMLDEITRVKWYPAWAGSARFHDFVKESRDWCI
                     SRQRYWGIPIPIWQCERCGGYTVIGTIAELEERSGKSVPDPHRPYVDGVTIPCPCGGE
                     MRRVADIFDVWFDSAVASWATLRFPRDREGFERYWPADFITEGQDQTRGWFYSQLGAS
                     TIAFGRAPYRSVLMHGFALDAEGRKMSKSFGNVVTPEEVISQFGVDVLRFYVLWSNAP
                     WDDLKFNWDGVKTIHRTLNILWNVYRFPLPYMVLDSFSPASADGTFVRAHISDMPDED
                     RWILSRVNSLVRATTAEMQDYNLHKVTRALANFILEDLSRWYVQVVRPRMWLEEDSPE
                     KLYAYETFYYVLRRLIALLAPFVPHITEEMYGNLRLEGDPESVHMLDWPVADEHLIDQ
                     RLESAMEVIRSFDDAVATARQAGKRKLRWPVAETVVVTENEAVKAAMEELNELARIRA
                     NSRSVRVVTGAWDRIRWKPEPVMRAIGPEFGKKAPRVKALIEQADGTALKTAIERDGK
                     ARLDGYEIEARHVTFSESLPEGVFAAPMKDATVYVDITLTPDLEAEGYAREVIRRIQE
                     MRRQLNLNVDDYIVAAVDVANERVASLIGEEKWKKEIAGEVRASDLVIRRTDGDRPGE
                     ESYTLDKTWDVEGVQMRIGISRAGE"
     assembly_gap    19269..19368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19402..19872
                     /locus_tag="PK196_01595"
     CDS             19402..19872
                     /locus_tag="PK196_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HON40150.1"
                     /translation="MQIAVIGRGDCSVEEYEAAETIGRLIAGNRETVCCGGLGGVMEA
                     ACRGAKEAGGTTVGILPDTGDGNPYLDLVIRTGMGQARNVILVNSADAVIAVGGGYGT
                     LSEIAIALKTGKPVFGLSTWDIDGVFACTTPEEAVNLAVRAARLSRRSRNPQDR"
     gene            complement(19811..21673)
                     /locus_tag="PK196_01600"
     CDS             complement(19811..21673)
                     /locus_tag="PK196_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HON40151.1"
                     /translation="MVKRYLSVISLEEARSLVERSFQAVPRVVRVPVGTELIGRVTAE
                     PIFARFSVPAIHISAMDGIAVRSADTVGASEQQPLTLPDAVPVNTGNIVPPGYDAVVM
                     IEDVWLESGRYTIRKPVSPWQHIRPVGEDIAESEMILPRGHRIRPHELGALANYGVTE
                     VAVKHLRAGLIPTGSELVPPGIMPPPGKVIESNTLLAAAILSEAGVEACRYPIVPDDH
                     NAIKGAVRRGIEENDILLVSAGSSAGTRDYTADVIGDLGEVLAHGVAIKPGKPVIIGR
                     VGGKPVIGLPGYPLAAVTVMREIVMPLVARYGLPVPRPEGLSARLTTSLHSDIGTDEF
                     VLLSVGMVGDRWVAVPQSRGAGIQMSAVRANGYMQIPAQKEGVEAGETVDVRLTVPRT
                     EAEEAVLITGSHDPSLDYLADLVRPRGVDLHSTHVGSMGGVIALKKRECHAAPMHLLA
                     PDGTYNTHYLRRYAPDADLVLLCVAERQQGVISREGLGFDDLPGRRFVNRQKGSGTRV
                     LLDYRLAERGIDPASIPGYDREVTTHLAVALAVKTGEADAGMGVYSAAKALDLAFVPV
                     ATERYELAMHRQTLDDPRVSALVETVSSETFKQILNDLGGYETAETGVLRELRG"
     gene            complement(21667..22869)
                     /locus_tag="PK196_01605"
     CDS             complement(21667..22869)
                     /locus_tag="PK196_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HON40152.1"
                     /translation="MSLFLEVVSVAEAIETVRRIAPPGGCEDVPLEDALHRVLAKDVC
                     ADIDVPGFDRSTVDGYAVAAADTVGASEAIPAMLQCLGRVSMGSTDPGSISPGSCRYV
                     PTGGALPAGADAVVMIENTEQIGDDLLVHRPVAPGENVIRRGEDFEAGEVVLERGRVL
                     TPREIGVAAAVGADRVSVVMVPRIGIISTGNEVVPIVDTPGPGQVRDANSYLAGAFVR
                     ERGCRPVYFGIVRDDRAALMRAVASALERCDAVLISGGSSKDERDNTAAVVAELGEVL
                     VHGIALAPGKPTIIGTARKKPVIGLPGHPASTFVVLVAIVDHLIAAMRQTTVSRRTLR
                     ARLTQNIPSTRGREDYIRVSLDDGRATPIFGKSGLLNTLVQSDGLVRIPASSEGLEVD
                     EEVEVILW"
     gene            complement(22866..23048)
                     /locus_tag="PK196_01610"
     CDS             complement(22866..23048)
                     /locus_tag="PK196_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40153.1"
                     /translation="MDDETIRRAFEAYGVTTEITCPQAFEISERYNIPKMDIARYCNQ
                     HEPRIKIRGCQLGCFR"
     gene            23144..24457
                     /locus_tag="PK196_01615"
     CDS             23144..24457
                     /locus_tag="PK196_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048063716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HON40154.1"
                     /translation="MQITLVNSKQDAAGANIRNNLKNLLETGGHWPLVEAHDLDFHEV
                     DGRLIHQDRIDDEVDADLIIFISRHSSMQPTPALTVHVTGNYGRADLGGEPRRLAPAS
                     PAWMHAVLRNLHARAPPGYRVSYEVTHHGPTELSTPSLFAEIGSGAAEWTDPVAGRAV
                     AESILSAVPEETINLIGFGGTHYAVRQTGIALSSRGAFGHIVPTREIDAVDAGILQNM
                     QEASAAVAAYIDRKSVSTDQGRRIERMLADAGLPILTESEILEASRLAWSTYLQVRDL
                     AEEIAPGSRVHIHSMRGEGKPVVIQVAPELVREALKSSRSGFLEALERLPIAHISQGS
                     TEVSSRFICFENDSSRLASDITTQCIKLLLICENAVIEGDHLVLRKVRFDPEKARRQG
                     VPKGPLYARLASGEAVEIEGRRITPDTVQTTSVKRIHIPGLERYL"
     gene            24454..25551
                     /gene="ftsZ"
                     /locus_tag="PK196_01620"
     CDS             24454..25551
                     /gene="ftsZ"
                     /locus_tag="PK196_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HON40155.1"
                     /translation="MKSIVEEALSRAGAEQRFAEPPEVDRDLEEILMELRTEIAVIGC
                     GGGGSNTVTRMWEEGINGARLIAINTDAQHLVRTRADTRILIGRQRTRGLGAGSIPQV
                     GEEAALENEDDIRRAVQGCDMVFITTGLGGGTGTGAAPIVAKAARDEGALTIAVVTLP
                     FTAEGAIRAQNAEAGLERLREVADTVIVVPNDRLLEVVPRLPLHAAFKVSDEVLMRAV
                     KGITELITMPGLVNLDFADVRTVMERGGVAMIGMGESDSEDKAADSVKKALRSPLLDV
                     DISGATAALVNVVGGPDMTMAEAEGVIQEVYDRIDPDARIIWGAQIDPEMQGKMRTLL
                     VVTGVRSPQIYGRSEKSKPRTIRQFEIDFLK"
     gene            25561..25767
                     /locus_tag="PK196_01625"
     CDS             25561..25767
                     /locus_tag="PK196_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HON40156.1"
                     /translation="MNYKEMLERIKSFKIEEELFKKYWRVLKLARRPTRDEFTKIAIV
                     SAAGILLIGLVGFVIYEIMLLLPR"
     gene            25773..26246
                     /locus_tag="PK196_01630"
     CDS             25773..26246
                     /locus_tag="PK196_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HON40157.1"
                     /translation="MAEGANRVYAVKTTAKQERTVADNIEKVTREQKDIHVTAVMVPE
                     ELKGYVLVESPDSLARMEQLVELIPHARAVVRGSTTLAEVEHFLVPKPVVSGITEGTI
                     VEIIAGPFKGEKAVVKRIDSGKEEITVELYESMVPIPITVRGDSVRVVERSEDAS"
     gene            26397..26873
                     /locus_tag="PK196_01635"
     CDS             26397..26873
                     /locus_tag="PK196_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HON40158.1"
                     /translation="MAETVEVLVPGGKATAGPPLGPALGPLGINVKAVVDEINRKTAE
                     FNGMQVPVTVTVDDKRNFTIEVGIPPTAALIKKEAGIERGSAEPGSQVVGDLPLEAVA
                     RIARMKFDGMLSYDLKSAVKEVAGTCMSVGVNIEGKKPREMIQAINNGEYDDVLVA"
     gene            26925..27566
                     /locus_tag="PK196_01640"
     CDS             26925..27566
                     /locus_tag="PK196_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HON40159.1"
                     /translation="MVDITSIQKAVKTALEKAPERKFRESVDLTVNLRNIDMSQPKNR
                     IDETVLLPHGFDNVKIAVLGKGDIVTQAKEANVDLILGPEDIERLGGEPREARKIADE
                     YRFFLAETAMMPLVGRYLGVRLGPRGKMPIPISQATDIRPIVQRLRNSVKIRTKDKKV
                     FHTKVGTTAMDPAEIAENIDTILRRVESVLESGAMNIHSVYVKTTMGPAVRVI"
     gene            27566..28594
                     /locus_tag="PK196_01645"
     CDS             27566..28594
                     /locus_tag="PK196_01645"
                     /inference="COORDINATES: protein motif:HMM:NF003098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HON40160.1"
                     /translation="MALYTHHLPRWKRDEVEEIKRGIQEHPVVGIVNMHGIPASQVQH
                     IRWNLRGTARVKMSRNTLIERALNELGGSVADLKEHFEGQSALIFTNENPFRLFKLLE
                     QTKTKMAAKPGEIAPEDIVVPKGPTTFKPGPIVGEFQQVGIPAVIEGGKVKIRETKTV
                     VKKGEVINRKVAEVLSKLGIKPMDVGLALQAAYYRGSVFTPDMLAIDEEAYANNIAFA
                     ARQAFNLSVNAAIPTRLTIEAIIGRAVNEARNVGVEAGVYEKDIIDLIIGRARREMLA
                     VALAAQARGFDLDEETLKAATAAAPAVPAAAPASAAAPAAEEEKKEEEKKEEDEESGL
                     AGLGALFG"
     gene            28621..28935
                     /gene="rpl12p"
                     /locus_tag="PK196_01650"
     CDS             28621..28935
                     /gene="rpl12p"
                     /locus_tag="PK196_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HON40161.1"
                     /translation="MEYIYAALLLHNAGKEITEENVTAVLKSAGVEVQDARVKALVAA
                     LEDVNIEEAINRAALAPVAAAAPAAAAAPAGAAAEEVEKEESKKEEDEESGLAGLGAL
                     FG"
BASE COUNT         5716 a         8932 c         8883 g         5490 t
ORIGIN      
        1 aagatcgtgg agaggcttga actgaacata ttccctaata tatgaggaga ttgggataga
       61 gaagtataac aacgatgcgt cgcatctctt cgatcctgac agtggagaaa acctcgaaac
      121 gagcgatctg tatcatttcc cgttcagtga gggatataga gtgctactcg aagcagcgca
      181 ggcggctgat ggtacgacag agccgtatca gtccgcatat gcgataacgc agtatgtcaa
      241 tggggctatg aattacacat acgaagaccc atatcttgaa tatatattct ccgacctgta
      301 catgatggat cacccctctc ccataaacgg aaagtatcaa ggggtctgtg atgagtatgg
      361 aaccctgtat accgccttcg ccagggcgct gggtatcccg acacggttct tatcgttcac
      421 catgagactg ccttccggta atataacggg gcatgccatt gcagaatcgt ggaacggaac
      481 tacctgggtt cactcggatc cgccatggaa tgtatttgac aatccccagg tttacagggc
      541 agcgggcaac gatcacatca acattaccgt ctacggtgac gccgacgact cccattacac
      601 acaggacccg aacgacccga ccggcgacgg tattctcagg tacgaagatt tcagaacaca
      661 gattctcctc ggagaggttc cgaggtacaa ttaacccaat ttttatgccg aaaagtattg
      721 tcctcctggc acttggtctc accgttgcgg tgctcgcctg cagcatccct gttggtgcgg
      781 tagtgacgcc cctccccggg cagggaaatc agacaggtat cctgtttggg gttcacccga
      841 acgcgaagac caacgagacg atcatcgcgt tcatcgccga acacggggag atctggtggc
      901 atgagggcac gtacaatcac acacatgagt acgtgtacgt cgtgccggcg aacgcctgcg
      961 aagaacagct cgtctattta aacaggatta gagccgatga acacggcggc gaacagtcca
     1021 tcgagcagat ctggatcatc ggctgcgatg aggcgatgcc cctggccgca gagctccggt
     1081 tcccggagat ctttgcggag tacccggcgc tcagcaacga cgaagggctg cggtcgtacg
     1141 tcgagaatca atccccgaac cgctatgagg agattacatc caaacctgcg cgagcgcagg
     1201 tctacttcat caacgatacc ggcgtgttca cggagtacat cgtcgacctc tcggggggag
     1261 agatcgcctc caccacctac gtctcggaag agaacctcac ggtcgggaga gccgaagcgg
     1321 aggcgcaggc cctctccgga cggtctccgg acgcccgcgt cgagaacacc atgctgaaag
     1381 tctacagcgg cgaccagatc gtctgggaga ttaccgtccg ggccggtcag aggacagacg
     1441 tcgtgcaggt cccctcggac ctggcgcccg ggcagtcgct ccggacgacg acgcccgggt
     1501 ttggagcggt cctcgcagtt cttggtgccg ggatgggttt tgttctgtac cggaggcgga
     1561 gatagaaccg gaacgacggg cagacctttc gagggaatct ttcgagggaa tcccgacgat
     1621 gggcggatgt gcaaccgtcc gggccaaccc caaaaaaata gttactcccc cttcagcatc
     1681 gcgttgatcg ccgccgccgc gcgcttcgcc gagcccatcg cctcgatcac cgttgcagcg
     1741 ccggtggcga tatccccgcc ggcgtaaacg tgagggatgg acgtccggcc gttcttgtcg
     1801 acaacgacgt tccccttccg cccgcgctcg agccccggga tcagggagac caggagcggg
     1861 ttcgggctcg tgccgatcgc ctggatgacc atgtccacgt cgagggtgaa ctcgctcccc
     1921 tcgatcggct ccggcgaccg ccggccgctt gcatccatct cgcagagaga catcttcacg
     1981 cactctatcc cggtgacacc gcctttgccc aggatccgga tcgggtttgt gcaggtgagg
     2041 aactcgatcc cctcctcctt tgcgtggacg acctccgccc tccgtgccgg catctcctcc
     2101 tcgccgcgcc ggtagatcag gtagaccttc gcgccaagcc ggcgcgccac cctggcagca
     2161 tccatcgcaa cgtttccgcc gccgatcacc gccacacgcc cggcgtgaag gacaggggtg
     2221 tcgtattcgg ggaaggcgtc ggcgtgcatc aggttcaccc tcgtgaggaa ctcgtttgcg
     2281 gagtagacgc cgtttaagtt ctcgccaggg atgcccatga acgccgggag tcctgcgccc
     2341 gttgccagga agaccgcgtc gtaacccagg agttcatcga gaccgacgct catccccacc
     2401 aggtggttct tcttcaaccg cacacccagg gcaagcacct gctcgatctc cgccctgact
     2461 atatccttcg ggagtcggaa cgccgggatg ccgtaggcca ggacaccccc cgccgcgtgc
     2521 agcgattcaa agatggttac ggcgtggccg gcgcgggcca gttctgccgc agcggtcagc
     2581 cccgccgggc ctgagccaac aaccgcaacc cgttttccgg tcggtctcgc cacgtcgggg
     2641 agggtcgggt tgttctgcct ctcccagtcg gcgacgaacc gctcgagtgc gccgatccgt
     2701 atcggcgtct ctttgttgcc gaggacgcag accccctcgc actgcgtctc ctgcgggcag
     2761 acccggccgc agatggccgg gagcatgttc tggtccttga gagaccgtgc ggccgccggg
     2821 aaattgccct ccgcaacctg atggacgaac cccgggatat cgatcccgac cgggcagccc
     2881 ttcacgcaga gcggtttctt gcactggagg cagcgttctg cctcggcaac ggcctcatca
     2941 ggggagagcc cgaggtccac ctcctcaaaa tcgtgaatcc ggatctcggc cggcctctca
     3001 cccatgctgc tctcccccct cgccgcagcg gcaccggtgc tctgcgaacc gctcgagcgc
     3061 ggtcttctcc tgcgcggtgt agatccgctg ccgctgcatc aggttgtcaa aatcaacctg
     3121 gtgggcatcg aactcggggc cgtcgacgca ggcaaacttt gtctcaccgc cgacgctcac
     3181 ccggcaggag ccgcacatcc ccgtcccgtc gaccatgatc gggttgagac tcacgtaggt
     3241 cttcacgccg gcgggcgccg tcacggcgga ggtgaccttc atcatgatgg cggggccgat
     3301 gatccagacc cggtcgatcg tcctctcctc gaacagtctc ttcaggacgt cgcttgcaaa
     3361 accgtggacg cccctgctgc cgtcgtcggt tgttatgtag agttcgtcgc agatctcgcg
     3421 catctcgtct tccaggatga gaaggtctgc gctccgcgct ccgatgatcc cgatgacatg
     3481 gtttccagcg cgtttcagtt ccgctgcgat gggaggcgtg ctcgcgatcc cgacaccgcc
     3541 gccgatcaca cagcatgtgc cgtagttatc gatctcgctt ggtcttccaa gcggtcctgc
     3601 gacgtccatt atgctctcgc cggcctggag ggtcgcaagc tcctcggttg tcttgcccac
     3661 ggtcataaat ataacccgga ctgcatctcc cctggccgcg gagatggtga gtggtatccg
     3721 ttcgcccttc tcgtgcaggc gaactatgag aaactgtcct gcccgtgcgt gccgcgcaac
     3781 gtgtggcgcg tggatccagt actcgtagac ccggtcggcg agcctggccg ctgattctat
     3841 cctgtacaac cgcttcactc ctgaatgatc ctggttggtc tgccctcaaa tgcagttaac
     3901 gcttcgtata cattgtggct ccagcctctt tactgcagct acctggtgcc attgaatcgc
     3961 ccccttcggc aagaggaggc agggagtttt gccctctcac atctcttccg catctcgcgg
     4021 tgaacgttca ttgtcgactg ccagcatccc ctgtctccac acgttttccg gacactctct
     4081 ttcctggatg atgagaccgc acccacctcc tgccgccttg agaggcgatt ggcccatgga
     4141 aaccgcggcc gggccgcaga cacgagaccc gcgaatgttt cagcgataac cgaagagacg
     4201 gtttcctacc cgacagaggt tcatcccacc gggacacagc cagaaggagc cccctattat
     4261 ggcctctccg ggcggcggaa tagaatgagc aaacggttat ccctctaaaa acgagagaaa
     4321 gagggtatgc aaagccagat aatctggggt ctcgccctga cgctcctcgg cggggtggtt
     4381 gcgggcgcgg cgattgcatg gctaccagtt ctcctcatat acgccgtccc gctcatcgcg
     4441 atgggggttg cgctcatcat ctggcgtgga cgcgaggaga tcctcgaacc tgtagaggag
     4501 tgaagagaga tgatactgac aacaactgca gaggttcctg gccacgagat cgaggagatc
     4561 ctgggtgtgg tctttggcaa cacagtacgg acgaagaaca tagggaaaga catcactgcg
     4621 gggctcaaga gcataatcgg cggtgaactc gaggagtata ccgatatgct caccgacgcc
     4681 cggacagagg cctacaaccg gatggtcaat ttcgcgcgtg aactcggggc cgatgcggtg
     4741 gtgaacatcc ggttcacgac gtcccagaca atgtcgacgg cggcagaact cctggcctac
     4801 gggacggctg tgagacttgt tccaaagaag tagttccggg gtttttgcaa ccctcaggtc
     4861 ttcttcacct tccgcaccct gaccgcaaca ccgcgctttg accggagcat ctcgtccgcc
     4921 cccatcatcg cacgcccggt ggcgaccgcg agaggccctt caaccagaac ctcgtccccc
     4981 tcccgtatcc gggggtcgca gtcaacaact cctggagcaa ggacatcacc ctgcggcacg
     5041 aactcatcta tcctgacacg gtaaccctcc gggataaggt cccagcccgc aaacgtcggg
     5101 cgcagcagcc ccgtcccctc gtcgatgctg aagatctgct ccctcccgcg gaggactgcc
     5161 atctgcatgc ttctccccct gacctggaga cccttcgtgt tgatctctgt tccgaactgc
     5221 caggaggccg tgcccttgat ggtatcggtc tgcacccttc gttcgcccgc gagagcctcg
     5281 tcgagcgccc ggagcgactc cggcgacgcc gggtggccgc ggcatgtcac ctcaagatcg
     5341 ataccgcagg cctttgccgc catctccgct acgctgagag cgccgccgtc cagatgggcg
     5401 atcacccggc ggtaggggtg ggcggcaaag tagcgggcca gtatatcggc gatgaacgcg
     5461 cactcctcgc ggtcccagta gccggtcacc ggcacgtcgt agtgcgccgc cgggtagacc
     5521 cgttcgagtt cccgtggcac aaggccaagc ggagaggtga cgatcagttc gtgcgcccgc
     5581 cggttcacag cgttcatgaa gagtttgtgg ctccgtgaaa gagagtaggg cttctttgcc
     5641 gagcagggaa ggagaaccgc gacgtcagtc ctggtcgggg cgaaccgctc gatcacgcga
     5701 tcggcaaacc gcctgatctc agcccggttc tgcgactcgg cggtggccgc aagcatcggc
     5761 gctgcccgcg caacaggaag gcggcgttcc atgaaagcgt agttccggtc gaggaaccgg
     5821 agggcggcca cctctgcagc gttcgcacgg caccgttctt ccaccagctc ccttagcctg
     5881 ccggtctcga tgtggtggcg gacgagggcg atctcgcggt cgagcgcgag acggttgtgc
     5941 cgccggaggt cgccggcccg gcacccctcg cagccgcaga caccgccatc catcagaccg
     6001 gcggggaact cgccctccgg caggcagaac ctcttctgcg cggaggcaag gtcgaccccg
     6061 cgatagtcga agatgtcaaa ccccgagtat accaggaggc agacggtcga tgggagcgct
     6121 gcagccggag cataccaggc ggtgtcggga gggagacgct cttttagaga ggcaagccag
     6181 gcggcgtagt tccggggact cgagagagcg gcctgccagt tcgcggcaag gacgcagtcg
     6241 cccgattctg ccgacgctga gagggggtgg acggcaacag gctccccatc gccgggggag
     6301 aagtatctct cgacgaaggc ctgatcggcg gcgagcggga cgtttgagag aggtctatcg
     6361 cggagagagg gaaacagggc atcagtatcg agcgcagcgg ggaggaaaac ggtcttcccc
     6421 ccatgctcgt aggcagatgc ccgtgcaaga ccgtcgcggc atcgagcctc atacctgctc
     6481 atggagcacc tccgcgcccg gcacctcttc ccggaccagc cgcgcccact catctgcgca
     6541 ccggacggca aactctttcc ccgggtttgc ctccaccaga gaccggatac cggcgcaccc
     6601 tgccctgacc atctcctcat cccactcagg ggtctcgctc tggcctatcg gaaacgtctc
     6661 cgcgagttcc ggggggtatg ggccgaacgg cggtttgaag ttcaggacgg tatcataccc
     6721 tggagccccg cggccgtcga acgagaccag cacccgcccg ccgaggggga tgcgggggat
     6781 gaccctgtgg tatcggagca cctctgtcct ccggcaggtc tcagcccccc cgtagaagaa
     6841 ccggcgcttg gagacggggt cgtcgacctc cagttcggcc gtgtgtccca ggagttcacg
     6901 gtagccgtcg agcaggcgcg ggtggctccg gcaccgctca tcgaccagtt cccatagagt
     6961 cccgtcctgg atcgcctgcc ggatacggga gatctcggcc agggtgacgt ggaggttgtg
     7021 gagagcgagc aaccgctcgc gatcttcgga cttccggagt tcgtcggcgg tcatggaacg
     7081 gcagacctgg caggggcagg ggagttctgc gagttcgtct atcttgaggc tcccgtgggt
     7141 ggtgatgtaa cggccttccc tggcaaagag agcgtaggcg gcagagtcga agaggtcgca
     7201 gcccatggca acggcgaggg cgaacatcga ggggtggccg gcaccgaaga ggtggacggc
     7261 gattgccggg gagagtccgc gttttgccgc gaggacgacc ctgacaaggt cacggtatcg
     7321 gtaggcctcc atcagcggca ccaccgcccc gatcgggcag aacgtcaacc cgagttccct
     7381 gacggcacgt cctgactctt cgcgcaggtc ggcgaagatc ccgccctgaa ccggcccggc
     7441 caggtttgcg tcggggaaga ggcggtgcgc ctcccggatc cgttccatcg tgacgccgag
     7501 gtcctgttcc gcccgctgcc gtcctgcgtc cgggggtgtt gggatgtcca ggggaacgat
     7561 gatatcgctt cctatcgcct gctggaactc gagcgtctcc ctgttgtcca cctcgacttc
     7621 accgtagacc gagagctgga acgaaccgga atcggtcatt atggcgccgt cgaaatcgag
     7681 gacacgatgg agtccttctg cgagtgcgcg gtcgcggtag tcagcgctcc gcctgaatat
     7741 gtaggcgttt gttatcagcg cctcgatgcc catcctcccc atctcgcggg gtgtgaccag
     7801 cgggaggtgg gggttgacca ccggcaggag cgcgggtgtc cggacggtct tttcgttcac
     7861 cctcagcctg ccgaccctcc cggcgatgtc tttgtggatg acctcaaacg tgattgtcat
     7921 tcaggttaga ccgccttctc ttcgataacc tggccctcaa acgtcagcac ggccacatcg
     7981 ccacgcctcc agcaccccgg cgggagcccc gccttgacac aggtctggtc gaggaactct
     8041 ctgctgttcc agccgtactc tgttgcgacc tgggggagga gaaggcctgc tctccccaga
     8101 ccgctgacta tcagaccgtg cctccctacc tccacgtgtt ccgggcgctc cagtggcggg
     8161 cactcgagcg gcctcggcgg tgtcagcacc gtcacctcga tctcgatgtc gtcgacctcc
     8221 agggatgaga ccggcggaaa ccgggggtcc tcgagtgctg ccgaggcggc cgcctcaata
     8281 atagcctcgc caagcggttt tatcgggtat gggaggccga tacatccgcg tagaagtccg
     8341 tgccgcttga tggtgacaaa cacaccacgt ttctccccga ataccggggg gagtgggggc
     8401 agcaccatcc gttcgccgtt gacggctctc tcgatggcca tccgcgccag gcgaaccgcc
     8461 attctgcctt cttctggggt cagcatttcc atcagaatgg gttgtatgat attgaatact
     8521 tttagcattt cgcgggagag ctggtggtga ggggaagatg cgttgcatgc agtcaccagc
     8581 gtaaacccga cctggaggct gctgccgata cagatgaacc atccacgagt ttctccactg
     8641 caaacaggga ggcgttatcg cgtccatctg agacgctcct ccagagacct gatccggtca
     8701 cgtgctggcg cgcgaagcgc cttacgctgt gtttaccagg tcccggggcg acccaccctc
     8761 ataaatatat accctcgcag caaaactatt ataggagaga gggagagcgc ggccgacggt
     8821 ggcacatctt gtgccgctga ggaaagtcct tccaccggcc agacacgcag ccgcacgcaa
     8881 gtgcgggtgg cgagagtcac ggcaatgaca cagaaacgac acggcccgcc gtcagtgatg
     8941 aaacgatcga gcccacacac cgcgggcgag cggcgcgggt ttattcccgt gcaactcgtt
     9001 gaacgcagcg gcgggaaacg atgaaacggt gaatccctgc gggtgcaagc cagaataggg
     9061 catatggact gtctggcgtc cgcccgggta tggcgcttag ctgaatgccg caccaaacag
     9121 aagaaggctt actcctccca ctctccatcc attctcggta gtgtgtgcaa tattatccct
     9181 caccagaatc ctctcgctgg tagactgtgc ggaaaaatcc ctctcaccat aagtgaccgc
     9241 acgcgagatc cggggttcac cgtgacgacc gctccgcccc ggcgacgtac ctgcctgtca
     9301 gaatagatcg ctcagaaaga gaaacaagaa ttgagtgttc aacgggcctg aagggatttg
     9361 aacccctggc ctacggatta agagtccgtc gctctgccag ctaagctaca ggcccacgta
     9421 tctgacctaa cagtatggta gtgaaaacgg tataagcctt atggatcatt ccatgatatc
     9481 gccggggata ggggatccaa ttaataccat ggaacgttac accattgtac gcatgcctga
     9541 ggcggtccag aacgtttcga aggaacgtgt caagtacatc atccttgggt gcgggagcac
     9601 cggctacaac gtggccgagg aactcgccaa ggagaccgag gaactcatca tcgtcgataa
     9661 ggacgaaaaa cgggtggaag acctccggga ccagaagtac aatgcacttg ttcgcgactt
     9721 ccgcaacccg ggcttcatgg atgggctgcc cgtcccggag gttgccttta tcctggcaaa
     9781 cgaccgggag gccaatctgg ccgccctcaa gaccatcaag agccgctacc cggcgaccta
     9841 tgtcatcgcc cgcgccatcg acgcggtcag cgtcgatctc ctccagcagg aaggcgctga
     9901 tgtagtcctc tacccccagg aggtggtggc gcgaaccgcc atataccata tcaggaaact
     9961 ccactcttca cggcttgccc tgcggctcta cgatctgctt gcctcctggg aggggacgct
    10021 ctgcatcgtc acccacttaa accctgatcc cgattcgata tcaagcgcca tggcgctttc
    10081 actgatagcg cggcatgcaa gccacaacaa actcaactgc cgtatcgtct atgaaggaga
    10141 tatcggtcat caggagaacc gggcgtttat aaacctcctg gacatcaaga tggagcgtct
    10201 cacacctgat atgctggccg agtgcaacta catcgctctg gtagactcgt ccggacccgg
    10261 cgtgaacaac aatctgccaa aatcgacccg ggtcaacata atcatagacc atcacaagaa
    10321 caacaccgaa ccgccatcca ccggagctga cttcatcgat atcaggccag gcgtcggcgc
    10381 cacggcaagc atcatgaccc aatacctgat ggagctcgat atcccggtga gcaaaacggt
    10441 ggccacagcg ctcctgtacg gtatcagggc cgatacacga gatttcaagc ggaacgtcac
    10501 cccccaggat ctcaactacg cggcgttcct cctccccctc acggatgcag atctcctgga
    10561 taagatcacc tccccatcgg tctcactgga gactatcgag atcataggca acgccatccg
    10621 gaaccggaag atcaatagcg gttacctctt ctcaaacgta ggttatatca ggaaccggga
    10681 cgccctcccg caggcggccg atatgctgat ccaccttgaa ggcgtgaaca ccgccctggt
    10741 ctatggcatc acagaccaga acatcgtcat atcggcccga aacaaggata tcaggctcca
    10801 tctcgggaac gttatggccg aggcgttcgg ctccatcggg gaggcgggcg ggcatgccac
    10861 gatggctgcc gccatgatcc ccctcagtta tttctcaatg gcaaaggata aagaaggtct
    10921 gctcgccctg ataatcgatc ccatcctcaa acgtttctcc gagatcgtcg gcctggacgg
    10981 cgagggtgaa gaatgaggtt tgaggactgg gagcctcact acaccgcgat tctggagtat
    11041 tttgggtttg aacgcgaggc agacgaggag gccgcccggg tgctcgcaga ccttgccggc
    11101 ggccgggacg acatcgcgct gcttgaagcg ctgatccggg ggaggaacgt tacggtctgc
    11161 gggaacgccc catctctccc ggcagaactt gaccggatcg agggggcggt gcttgccgcc
    11221 gacgccgcgg cggaggtgct tgccggtcac ggagtgcggc cggacgcggt ctttacagac
    11281 ctggacgggg cgacagatct cttcatagac ctctcccggc agggaacggt gatggttgtc
    11341 catgcccacg gtgataacat cccgctcctc cgccactggg tcccccgcat acccgggccg
    11401 ctggtggcaa ccacccaggc ggctcccatt ccgggcgtcc acaactttgg ggggttcacc
    11461 gacggtgaca gggcggtctt tgccgccagc gacctcggcg cggcagacgt ccggatcatc
    11521 gggttcgatc tcgccgaccg ggatgtcgac cccctcaaga ggggaaaact ctactgggcg
    11581 ggcgaactcc tcaggatgat ggggtatgac ctctgaggcc cttataatcg acggttacgt
    11641 cgatgaaccc gcgtgtctcg gtgtcccgcc atacatatcc ccctacatcc gcgaggtcgc
    11701 cggcgtcctg gccgcgcatg gctacgcgcc acgctacgcc accatcgatc aggtccgggc
    11761 caacccgtcc cttctcgccg gtcacggccg cggcgatctg gtggtgatga tcgccgggct
    11821 gaccgtgccc ggcgcttaca taggcggtcg acctgcaacc ctgacggaga tccagcagat
    11881 cgggctcacc ctccgggaac cggccaccgc gatcggcggc ccgatcaact tcgggtatgc
    11941 tccagggggt gggaggaagg caatccggca ggcgatagcc gggtttgatg cagttctccg
    12001 cggctctccg gctgttgcgc tggactcctg gctctcgggg ggtgaaccgg aaggtgagcg
    12061 ggattacgcc ctgagcgatc cctggtgcgc tgctggtgcc gggatcatag cgcagcaccc
    12121 ctcgttccct cacgtaatct gcgagctgga gactgcaacc ggttgccccc gtgcaacggt
    12181 cgggggatgc tcgttctgca ccgagccttt ctacggcttc ccgcgctacc ggagcatcga
    12241 tgggatcgcg gatgaggtgg cggcgctcca cgccgccggt gcccgccatt tcaggctcgg
    12301 ccgccagccg gaccttcttt cctaccggag caccggtggc ggcgagttcc cgctcccccg
    12361 gcccgaggcg ctcgaagaac tcttctcggc ggtaagagag aacgcacccg gccttaagac
    12421 cctccacatc gacaacatca accccggcac catcgccaga catccggacg cggcacgggc
    12481 ggcgctcgag gtgatagtgg ccgggcacac acccggcgac accgcggcat tcgggatgga
    12541 gaccgcggac ccggcggttg tcagggcaaa caacctcaaa gccatgccgg aggacgtatt
    12601 ccgcgccatc gaggtggtga acgaggtcgg ggggaggcgg acccgcggca tccccgaact
    12661 cctgccgggc ttgaacttca tcatcggtct tgccggcgag acgccggcga cgtttgatgc
    12721 aaacgaagcg ttcctccgcc gcgtgctcga cgccgggctg cttgtgcggc gggtgaacat
    12781 ccggcagttg atgccttttg aggggacggc cgcctacgag gagaacaccc tggggaagca
    12841 cgatgcccgg ttcagggcgt tcaaggattg ggtcaggctg gagttcgacg aaccgatgct
    12901 gcgccgggtc tttccggtcg gcagcattct caaggaggtg gtcatcgagg tctccgggag
    12961 accgtcgttc gggcggcaga tgggttcata ccccatcctt gtcgggatcc cgctcgaact
    13021 ccctgtgaga acggtcatcg acgctgtcgt ggtcgattgg gggagtcggt cggtgaccgc
    13081 cctcccgtat ccggtggaga taaacaccct cccgccggcg gcgctccgct ggatcccggg
    13141 catcggcagg aagagggccg caagcattgc cgcccgccgg ccgttttcga gcctggagga
    13201 gttccgggcg gtcgtggggg agacggggct cgatgatctg atcatgtttt agagccccac
    13261 acctctcccg tgcgtgaaga gttccagact gctgcccatc aaacgggcct gacccccgcc
    13321 ggccggtgcg ggaattgccc gggaggggct gcccacttac gccttctccc gcagttccat
    13381 aacccggagc acgtccgtcc ttgttacaat ccccacaaga tcgccgtcat caacgaccgg
    13441 gatccttcct atgccatggg tggtcatgat ccggagcgcg tcgatgagcg gtgctgatgg
    13501 cgggagtgtt atcgggtccc gggtcatgac gtcacgcacc tgcatcgcct cccggtcgat
    13561 cggtgagacc ttgtgaatgt cgctgagtgt aacaatcccg acgagattcc ctcgctcaac
    13621 cacagggaac ccgagatgct tcgtctcata cataagttcc agcaaccggg ggagcggggt
    13681 ctctggctcc acggtgacta gagagttgct catagcgtcc gccacggtga cgtcctgcag
    13741 caggacgtag tagcgcagga atgtggcctc ctggtttgcc ccgatgtaga tgaagaacgc
    13801 gattaagatc aggatggggt tcaacatcag gaacccgaag attccgaaga gaacggcaaa
    13861 gcccttcccc acatcggcgg cgatctgggt tgcgcgcgag agcggcatcc gccgcgcgag
    13921 ccacgcccgc agcacgcgcc cgccgtccat cgggaacgcc gggagcaggt tgaacgcgaa
    13981 gagcaggacg tttaagatcc caaggtagcc gaaggtaaag atcaggacgc cggctacggc
    14041 cgggtcagga aggacgggga agacgtagac cagggcgctg catatgatac ctatcgccag
    14101 gcttgtgagc ggcccggcta gcgccatcgg gagttccacc ttcggatccg gcacaacctc
    14161 ctccatctgt gagaccccgc ccaggataag gagcgtgatg ccgtggatct tgatcccttt
    14221 cgctttcgct atgagtgaat gggccacctc gtgaacaaat acgccaaaga atagacccag
    14281 cgcgacgacg gttccgagga tgtaggggtt aagtcctgct gtgatcagcg tcgcatcgat
    14341 cgggacacca aagaggatcc tgatcaactc ggtcgtgagc acgatctgac tcccgatgat
    14401 ccaggcaaat agagggatca ccagtagaaa actccagtgt attttgaccg gtattccagc
    14461 gatgttcccg atctgcagcg acgtttccat aaaatgagta tctttttatc atttatagta
    14521 atataccttc agaggataca gatgagaaca caggtcacag agatcttcgg attgccggtc
    14581 tacaccgaca aggctgtctt tatcgggaat gttgacgatg ttgtgctcga cgtcgaccag
    14641 aagaagatag ccgccctcgc gatcggcgac ctcaaccccg agatcgggga ggtgaagggc
    14701 tacaccgggc tgcagatccc cttccgcatc gtaaagagcg tgggggatgt cgtcatcgtc
    14761 cgtcacatcc ccgggctctt caggtcgccg gcaaaagaag agcaaccgtg attgcccggc
    14821 ccgcccggat aacgtctgcc tagatatgga tatcagagat cgggagatct tctcaaacct
    14881 gactatcttc ccccccgttt tcgtcaggct cgacggccgg gcattccacc gagtggcgcg
    14941 tgcgctcaac ctcaaaaagc cgttcgaccc ttctttcagc gcgagcatgc gagcggtctg
    15001 ccgctacctt ctcaccggga gcggactttc cccggtcttc gcctacacct tctccgacga
    15061 gatcagcctc tatttcaaga cgctgccgtt ctccgggcgg gtggagaaac tcgactccgt
    15121 gatcgccgcg gctgcagcaa gcaaactaac gatcgaggtc ggctgcaccc ggccgctggc
    15181 gttcgatgcc cggacaatcc cggcggccgg tgagtttgcc gtagagtatc tcgtatccag
    15241 acagaacgag gcctggcgca accacatcaa cgcctactgc cagagcgcgc ttatggagga
    15301 gggtatgacg tcacggcagg ccgccgcggc gctccggggg atgcgctcag aagagatgca
    15361 cgagatgatg tttgagcgcg gcatcaacct ggcagcgacg cctgcctggc agaggcgcgg
    15421 gacgctcctc taccgggaag agtgcacgaa agaagggtat aacccggtga cgggggagac
    15481 cgtccaggtc atgcggacgt gcatccgtga actggaggag acgcctctct tctccaatcc
    15541 ggagggggca gcgctgatcc ggtcactcac cggcgcgtga gatgccgatc cgcatctgca
    15601 caccctccac gtcccaggtc ttatcaaggg tgtaagactc ttctcccggc cggtctccgt
    15661 ccgtgcggcg gatgacgagg tcggatgcgc gcacctcgcc ggctatctcc ttcttccact
    15721 tctcttcccc gatgagcgat gccacccgtt cgttggcgac atccacggcc gcgacgatat
    15781 agtcgtcgac attaaggttt agctggcggc gcatctcctg gatccgccgg atcacctccc
    15841 tggcgtatcc ctcggcctcc aggtcagggg tgagtgtgat atcgacgtat accgtcgcat
    15901 ctttcatggg ggcggcaaat acgccctccg gcagggattc cgagaaggtg acgtggcgcg
    15961 cctcgatctc gtagccgtcg agcctggctt tcccgtcccg ttcgatggcg gtcttcagcg
    16021 cggtgccgtc cgcctgctcg atcagcgctt tgaccctcgg tgccttcttg ccgaactccg
    16081 ggccgatggc ccgcatcacc ggttcaggtt tccagcggat ccgatcccac gccccggtga
    16141 caacccggac ggagcggctg ttggcccgga tcctggcgag ttcgttcaac tcttccatgg
    16201 ccgccttaac cgcttcgttt tcagtgacaa ccaccgtctc ggcgaccggc cagcggagtt
    16261 tccgcttccc ggcctgccgg gctgtggcga ctgcgtcgtc aaacgaccgg atgacctcca
    16321 tggcggactc aagccgctgg tcgatgaggt gctcgtctgc caccggccag tcgagcatat
    16381 gaacgctctc cgggtcgccc tccaggcgca ggttgccata catctcctcg gtgatatggg
    16441 ggacaaacgg tgcaaggagc gcgatcaggc ggcgcaggac gtagtagaac gtctcgtatg
    16501 cgtagagttt ctccggtgag tcttcttcca gccacatcct gggcctgacg acctgcacat
    16561 accagcggga gaggtcttcc aggatgaagt ttgcaagcgc ccgcgtcacc ttatggaggt
    16621 tgtagtcctg catctctgcc gtagttgccc ggacaagcga gtttacacgg gataggatcc
    16681 agcggtcctc atccggcata tcgctgatat gggcccgaac gaacgtcccg tccgcggatg
    16741 ccgggctgaa ggagtccagg accatatagg gaagcgggaa ccggtagacg ttccagagga
    16801 tgttgagcgt ccggtgtatg gtcttcacgc cgtcccagtt gaacttcagg tcgtcccagg
    16861 gggcgtttga ccagagaacg tagaaccgga gaacgtcgac gccgaactgg cttatcacct
    16921 cttcgggtgt cacgacgttc ccgaaactct tgctcatctt gcgcccctcg gcatcgagag
    16981 cgaacccgtg catcaggaca ctcctgtaag gtgcgcggcc aaacgcaatg gtgcttgcgc
    17041 cgagttgaga gtagaaccag ccccgggtct ggtcctggcc ctcggtgatg aagtcagccg
    17101 gccagtagcg ctcaaacccc tcgcggtcgc gcgggaacct gagagtcgcc caggacgcta
    17161 ccgccgagtc aaaccagaca tcgaatatat cggcaacccg gcgcatctcg ccgccgcatg
    17221 ggcacggtat ggttacgccg tcgacatagg gccggtgggg gtcggggacg ctctttcctg
    17281 accgctcctc gagttcggcg atggtgccga tgacggtgta gcccccgcac cgttcgcact
    17341 gccagatggg gatggggata ccccagtagc gctgccggga gatgcaccag tcgcgcgact
    17401 ctttgacgaa gtcgtggaac ctggcgctcc ccgcccatgc ggggtaccac ttcaccctcg
    17461 tgatctcatc gagcattgta tcgcggatct cggtgacctt caggaaccac tgcgctgtcg
    17521 cgcggtagat gatgggggtc ttgcacctcc agcagtgacc gtagcggtgt gtgatcgact
    17581 tcctggcgag gagggaactg ccgagcgctt cgatgatgat atcgtttgcg tcccggacgt
    17641 acatatccgc aaaaatcccg gcctcgggag tgaaacagcc cccggtgtcg accgggcaga
    17701 agacttcaag cccttcccgg atgccgatca ggtagtcgtc ccagccgtgc ccgggcgcga
    17761 tgtggacgag accggtgttc tcgagttcaa cgaaatcggc ggcgacgata cggtgctgga
    17821 tctcggcctg gcgggggaca tctcttgcga ggggggaggt gtactccgtc ccgatgagtt
    17881 cagtcccgct gtggcgttcg aggacgctgt agtcctggta gcgcccaagt ttgaggacgg
    17941 actcaacgag ttcgtcggcg atccagagga tctcctccgc tccgtccttc tttgctgcca
    18001 cgcgcgcata ggtgaacgcc gggttgaccg ccaccgcaac gttcgccggc agcgtccagg
    18061 gtgtggtcgt ccagatgacc aggtactcct tatcgcgccc ggtgatgggg aacttgacaa
    18121 agatggaggg atcggtctcg tcccagtatt cgacctcgga atcggcgatc gccgtctcgc
    18181 agcgggggca ccagttcacg acccggtatc cgcgctcaag gagtccgcgc tcctctgccc
    18241 gctggattgt ccaccaggcg gactcgatgt actcttcctt gatggtctgg taggggtcat
    18301 cgaagtcgag ccagacgccg agacgccgga actgttcgct catgacctcc atgtgagtca
    18361 ccgcaaacgt ccggcactgc tcgatgaact gggcgatccc gtagtcctcg atatccttct
    18421 tggacgtgaa cccgagctgg tgctccacct tcacctcgat cgggagaccg tgcatgtcgt
    18481 agcccgcccg ttcgatgatc ttcctgccca gcatccggtg gtagcggagg atggcgtctt
    18541 ttatgatctt gttccaggcg gtgccgaggt ggatgttgcc ggtggtgtat ggcggcccgt
    18601 cgacgaaaaa gaaagcatct ccatcctttc gcatctcctg tacacgtgga tagatattct
    18661 cccgtctcca gcgatcctgg acacctgcct cgatctcctg cgggttataa ctggcggtga
    18721 cttctttcac cttgtatccc tcatggtcag cactctggtg tacctcttgt ccttgatgga
    18781 agaaagcgtt ttgcgcgacg ggacgcctgg cacgggtttg aacctcgtcg cccgcggggg
    18841 aaggagatat cgaggaaggt ttcagcgata accgaagaga cggttccacg gcacagggct
    18901 gcatttcggt gtggttcgag gagagcatct ctcctcaggg cacccccatc tccggccgtt
    18961 ctcgcgccat ctccccgcca ccttcgccgg gggatgggca gtgtatggtg attgacccgg
    19021 cggccgtgca cgggatggga aacagcgacc cagggaattt tctcaccgag aacgtggcgg
    19081 agtctcctcc ccgacactgt atcaatggag aatcgcctcc tggggtgggg ctgacgtgga
    19141 gggtggcgga gacgtctccc catccaccat ctagcccgcc tccgataaac gtctatcgtc
    19201 gacaaccccc cgcccttttt atcctccgcc cccggaagat tcatccatgc agatcgcagt
    19261 catcgggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tttttatcct
    19381 ccgcccccgg aagattcatc catgcagatc gcagtcatcg ggagaggaga ctgctccgtg
    19441 gaggagtatg aagcggcaga gaccataggc cgcctcatcg ccggcaaccg cgagaccgtc
    19501 tgctgcggcg ggctcggcgg tgtgatggag gccgcctgca ggggggcaaa agaggccggc
    19561 gggacgacgg tggggatact ccccgacacc ggggacggga acccctacct agacctggtc
    19621 atccgcaccg ggatgggcca ggcgaggaac gtcatccttg tcaactcggc cgatgcggtg
    19681 atcgccgtcg gcggaggcta cggcaccctc tctgagatcg ccatcgccct caagaccggt
    19741 aaaccggtct tcgggcttag cacctgggat attgatgggg tgtttgcctg cacaacaccc
    19801 gaagaagcgg tcaacctcgc agttcgcgca gcacgcctgt ctcggcggtc tcgtaacccc
    19861 caagatcgtt gaggatctgt ttgaacgtct ccgaggagac cgtctccacc agcgccgaga
    19921 cccgcgggtc atcgagcgtc tggcggtgca tcgcaagttc gtagcgctcc gttgccaccg
    19981 gcacaaacgc aagatcaagc gcctttgcgg cactgtagac gcccatcccg gcgtccgcct
    20041 cccccgtctt cacagccagc gccaccgcaa ggtgcgtcgt aacctcgcgg tcgtagccgg
    20101 ggatggaggc cgggtcaatg ccgcgttctg ccaggcggta gtccaggagc acccgtgtcc
    20161 ccgacccctt ctgccggttg acgaaccgcc gccccggcag gtcgtcgaac cccagaccct
    20221 cgcgggagat gacgccctgc tggcgttccg caacgcagag gaggacgagg tcagcgtcag
    20281 gggcgtagcg tctcaggtag tgggtgttgt aagtgccgtc gggggcgagg aggtgcatcg
    20341 gcgcggcatg acactcgcgt ttcttcaggg cgatcacgcc gcccatgctc ccgacgtggg
    20401 tggagtggag atcgacgccg cgcggccgga caaggtcggc aaggtagtct agcgaggggt
    20461 cgtggctccc ggtgattagc accgcctcct ccgcctccgt ccgcggaacg gtcagccgga
    20521 cgtccacagt ctcccccgcc tcgacaccct ccttctgtgc cgggatctgc atgtatccgt
    20581 tcgcccgcac cgcgctcatc tggatccccg caccccggga ttgcggcacc gcaacccagc
    20641 gatccccgac catccccacg gagaggagga cgaactcgtc tgtcccgata tccgagtgga
    20701 gactcgtcgt cagcctggcg ctgagacctt ccggccgggg gacgggtaga ccataccggg
    20761 caacgagcgg catgacaatc tcgcgcataa ccgtcaccgc agcgagcggg tagcccggga
    20821 gcccgatcac aggtttcccc ccgactctgc cgatgatcac cggtttaccc ggtttgatgg
    20881 ccacgccgtg tgccagcacc tcgccgagat ccccgatgac gtcggcggtg tagtcccgtg
    20941 tcccggccga cgatccagcc gatacgagca ggatgtcgtt ctcttcgatg ccgcgcctga
    21001 cagcgccctt tatggcgttg tggtcgtcag ggacgatggg gtagcggcag gcctcaaccc
    21061 ccgcctcaga gaggatggca gcggccagga gcgtgttgct ctcgataacc ttccccggcg
    21121 gcggcatgat gccgggcggg acaagttcgc tcccggtggg gatcagccct gcccgcaggt
    21181 gcttgaccgc aacctccgtc accccgtagt ttgcaagcgc cccgagttcg tgcggccgta
    21241 tccggtgtcc ccgcgggagg atcatctccg attcggctat atcctcgccg accgggcgga
    21301 tgtgctgcca ggggctgaca ggtttgcgga tggtgtatct cccgctctcc agccagacgt
    21361 cctcgatcat gaccacagcg tcgtagcctg gcgggactat gttgcccgtg ttgaccggca
    21421 ccgcgtccgg gagggtcaac ggctgctgct cgctggcccc gacggtatcg gcgctcctga
    21481 ccgctatccc gtccatcgcc gagatgtgga tcgcggggac cgagaacctg gcgaatatgg
    21541 gttcggcggt gacccggcct atcagttccg tccccacagg aacccggaca acccgcggaa
    21601 cggcttgaaa cgacctctcc accagcgacc gcgcctcttc aagcgatatg accgagaggt
    21661 agcgcttcac cagaggatca cctcgacctc ttcatcgacc tcaagcccct cgctcgaggc
    21721 cgggatccgc acaagaccgt cgctctgcac cagggtgttg aggagccccg acttcccgaa
    21781 gatcggtgtc gccctgccgt catcgaggct gacccggatg tagtcctccc tcccgcgggt
    21841 tgaggggatg ttctgcgtca gcctggcccg gagtgtccgg cgggagacgg tggtctgcct
    21901 catcgccgca atcaggtggt ccacgatggc gacgagcaca acgaatgttg aggccgggtg
    21961 ccccggcagc ccgatcaccg gttttttacg cgccgtccct atgatggtcg gtttccccgg
    22021 ggcaagggcg atcccgtgca ccagcacctc gccgagttcc gcaacaaccg cggcggtgtt
    22081 gtccctctca tcctttgacg accccccgga gatcaggaca gcgtcgcacc gctccagcgc
    22141 cgacgcgacc gcccgcatca acgccgcgcg gtcgtcccgg actatcccga aatagaccgg
    22201 gcggcagccc cgttctctca caaacgcgcc cgcaaggtag gagtttgcgt cccgcacctg
    22261 gcccggcccc ggcgtatcga ctatggggac gacctcgttg ccggtggata tgatcccgat
    22321 ccggggcacc atcaccacgc tgacgcggtc tgccccgacc gctgcggcga caccgatctc
    22381 cctcggcgtg agaacgcggc cgcgctccag taccacctcc ccggcctcga aatcctcgcc
    22441 cctgcggatc acgttctcgc cgggcgccac cggccggtgg accaggagat cgtcaccgat
    22501 ctgctcggtg ttctcgatca tcaccaccgc gtccgcaccc gccgggagtg ccccgccggt
    22561 cgggacgtag cggcacgacc ccggggatat gctcccagga tcagtgcttc ccatactgac
    22621 ccttcccagg cactgtagca tcgccggtat ggcctcggag gccccgactg tatcggccgc
    22681 ggcgacagcg tagccgtcga ccgttgagcg gtcaaacccg gggacatcga tatcggcgca
    22741 tacgtccttt gcaagcacgc ggtgaagcgc atcctcaagc gggacgtcct cgcaaccccc
    22801 tggaggagcg atcctccgca ccgtttctat agcctcggcg accgatacga cctcaagaaa
    22861 gagactcatc tgaagcagcc cagctggcag ccgcggatct ttattctcgg ttcgtgctgg
    22921 ttgcagtagc gcgcgatatc catctttggt atgttgtatc tctcggatat ctcgaaggcc
    22981 tggggacagg tgatctcggt tgttacacca taagcctcga atgctctccg tatcgtctcg
    23041 tcatccatga tactcagatc gtctgtctgc aggaagatag gatttactat cgtatccagg
    23101 tcgggcacta ccatttagca ctcacacgat ccttgtatac ctgatgcaga tcacgctggt
    23161 aaactcaaaa caggacgctg caggagcaaa catcaggaat aatctcaaga atctgcttga
    23221 aaccggtggg cactggccgc ttgtggaggc tcatgacctg gacttccatg aggtcgatgg
    23281 ccggttgatc caccaggaca ggatcgacga tgaggtcgat gccgacctga tcatatttat
    23341 atccaggcac tcgagcatgc aaccgacacc cgctctcaca gttcacgtga ccggaaatta
    23401 cgggcgtgca gaccttggcg gggaaccccg ccgcctcgcc ccggcatctc cggcctggat
    23461 gcacgccgtc ctccggaacc ttcatgcccg cgctccgccg ggctaccgcg tctcctacga
    23521 ggttacgcac cacggcccga cagaactctc aaccccctcg ctattcgccg agatagggtc
    23581 cggggccgca gaatggaccg accccgttgc cgggcgggcg gtggcagaga gcatactatc
    23641 tgccgtgccg gaggagacga tcaacctgat agggttcggg gggacgcact acgccgtccg
    23701 gcagaccggg atcgcactat catcccgcgg cgccttcggc cacatcgtgc cgacccgcga
    23761 gatcgatgcc gtggacgccg ggatcctcca gaatatgcag gaggcaagcg ctgcggtggc
    23821 cgcctacatc gacagaaaat cggtatccac cgatcagggc aggcggatcg agcggatgct
    23881 cgccgacgcc gggcttccca tcctcacgga gtcggagatc ctggaggcgt cgcgcctagc
    23941 atggtcaacc tacctccagg tcagggatct tgccgaagag atcgcaccgg gctcacgcgt
    24001 tcacatccac agcatgagag gtgaagggaa gcccgttgtg attcaagttg cccccgaact
    24061 ggtcagggag gcactcaaat ccagcagatc aggttttctt gaggcgcttg aacgattgcc
    24121 cattgcccat atctcgcagg gctcaacaga ggtctcatca aggttcatct gctttgagaa
    24181 tgattcgtcc cgactcgcaa gtgatataac tacccagtgt ataaaactcc tacttatctg
    24241 tgagaatgct gttatcgagg gtgatcacct cgtcctccgc aaggtgcggt ttgaccctga
    24301 gaaggcgcgc aggcaggggg tgccgaaggg gccgctttat gccaggcttg ccagcggaga
    24361 agcggtcgag atcgaggggc ggaggatcac gcctgataca gtgcagacaa ccagcgtgaa
    24421 acggatacat atcccgggat tggagagata cttatgaaat ccatcgtaga agaagctctt
    24481 tcacgggctg gggcggagca gcgctttgct gagccaccgg aggttgacag ggatctcgaa
    24541 gagatcctga tggagctccg gaccgagatc gcggtcatcg ggtgcggggg tggcggctcg
    24601 aacacggtga ccaggatgtg ggaggagggc atcaacggtg cacggctgat cgccatcaac
    24661 accgatgcac agcacctcgt ccggacgcgt gccgatactc gaatcctgat cggccggcag
    24721 agaacacgtg gccttggcgc gggctcgatc ccccaggtcg gcgaggaggc tgcccttgag
    24781 aatgaggacg acatcaggcg tgccgttcag ggctgcgata tggtcttcat caccaccgga
    24841 ctcggcggcg gcaccggcac cggtgctgca ccgattgtgg caaaagccgc gagagacgag
    24901 ggtgcgctga ccatcgcggt ggtcaccctg ccgttcaccg cggaaggtgc tatcagggcg
    24961 cagaacgcgg aagcagggct tgaacgcctc cgtgaagtgg cggacaccgt catcgtcgtt
    25021 ccaaacgacc ggctgctcga ggttgtgccc cgtctcccgc tccatgctgc cttcaaggtc
    25081 tctgacgagg tgctgatgcg ggctgtcaag ggcataaccg agctgatcac gatgcccggt
    25141 cttgtgaacc tggactttgc cgacgtccgg acggtcatgg agcgcggagg cgtcgcgatg
    25201 atcgggatgg gtgagagcga cagtgaggac aaagccgctg attcggtcaa gaaggcgctg
    25261 cgttccccgc tgctggatgt cgatatctcc ggcgcaaccg cggcgctcgt caacgtcgtc
    25321 ggtggccccg atatgacgat ggccgaggcg gaaggcgtca ttcaggaggt atacgaccgg
    25381 atcgatcccg acgcccgcat aatctggggt gcacagatcg accctgagat gcagggcaag
    25441 atgcggacgc tgcttgtggt caccggcgtc cggtcaccac aaatatatgg tagaagcgag
    25501 aagagtaagc ctcgtacgat aagacagttt gagattgact tcctgaagtg agccgttaat
    25561 atgaactata aggagatgct ggagcggata aagtcgttca agatcgagga ggaactcttc
    25621 aagaagtact ggcgcgtgct gaaactggca cggcggccta cccgtgatga gttcaccaag
    25681 attgctatag tatcggcggc aggcatcctg ctcatcggtc ttgtcggctt tgtaatatac
    25741 gagatcatgc tgctactgcc cagatgatgg atatggctga gggtgcaaac agggtttacg
    25801 cggtcaagac gaccgccaag caggagcgga cggtagccga caatatagag aaggtgacga
    25861 gggagcagaa ggatattcat gttacggccg tcatggttcc cgaagagttg aaaggctacg
    25921 tcctggtgga gagcccggac tcccttgctc gaatggagca gctggtggaa ctgatccccc
    25981 acgcgcgcgc ggtggtgcgc ggttcgacca cgcttgccga ggtggaacac ttcctggtgc
    26041 caaagccggt tgtcagcggc atcaccgagg ggaccatagt cgagatcatc gccgggccgt
    26101 tcaagggtga aaaggcggtt gtaaaacgta tcgattccgg gaaggaagag atcacggttg
    26161 agctctacga gagcatggtt ccaattccga tcactgtccg gggcgactcc gtccgcgtcg
    26221 tggagcgctc tgaagacgcc tcctgaagaa ggactcagtc ttttttttgt gtggtgcagg
    26281 ggatgatgcc atgcgcgccc aacgcccggg gttatgccct atactttaaa tcctctcagg
    26341 ttaaccttag ataacccagg caactattcc cctgaggaat aagctggtga gtttgtatgg
    26401 cagaaacggt cgaggtattg gtacccggcg gcaaggcaac tgcaggcccg cctctcgggc
    26461 ccgcgctcgg tcctctcggt atcaacgtga aggctgtcgt cgacgagatc aacagaaaga
    26521 cggccgaatt caacggcatg caggtgccgg tgacggttac tgtcgacgat aagcggaact
    26581 tcactatcga ggtgggtatt ccgcccaccg cggcgctcat caagaaagag gccggtatcg
    26641 agaggggttc ggctgagccg ggtagtcagg tggtaggaga cctgccgctg gaggccgtcg
    26701 cccgtattgc ccgtatgaag tttgatggta tgctctcgta cgacctgaag tcggcggtca
    26761 aggaggtcgc cggaacgtgc atgagtgtag gtgtcaacat cgaggggaag aaaccccgcg
    26821 agatgatcca ggccatcaat aacggggagt acgacgacgt actcgtcgca tagatgtcag
    26881 acaaccatag aagatcgtga ggtcgtgtac tatggaggca aaggatggtt gacataacca
    26941 gtatacagaa agccgtgaaa acggcactgg agaaggctcc ggaacgaaag ttcagggaga
    27001 gcgtggatct caccgtcaac ctcaggaata tcgatatgtc tcagccaaag aaccgtatcg
    27061 atgagacggt tcttctcccg cacggttttg acaacgtcaa gattgctgtt ctcgggaagg
    27121 gggatatcgt tacgcaggcg aaagaggcaa acgtcgacct catcctgggt cctgaggata
    27181 tcgaacggct cggtggcgaa ccacgggagg ctcggaagat cgcggatgaa tatcggttct
    27241 tcctggctga gacggcaatg atgcctcttg ttggccgcta tctcggtgtc cggctgggtc
    27301 cgcgtggaaa gatgccgatt ccgatctcgc aggcaaccga tatccggccc atcgttcagc
    27361 gcctgaggaa ctccgtgaag atccggacga aggacaagaa ggtcttccac accaaggtcg
    27421 ggaccaccgc gatggatcct gcggagatcg ctgagaacat cgatacaatt ctccggcggg
    27481 ttgagtccgt cctggagagt ggagcgatga atattcattc ggtctacgtg aagacgacga
    27541 tggggccggc agtgagggtg atctgatggc actttatacg caccatctgc cccggtggaa
    27601 gagggatgaa gtagaggaga tcaagcgcgg catccaggag cacccggtcg tcggtatcgt
    27661 gaatatgcac ggtatccccg cctcgcaggt gcagcatatc cggtggaacc tccgcggcac
    27721 agcgagggtg aagatgtccc gcaacacgct catcgagcgc gccttgaacg agctcggcgg
    27781 tagcgttgca gacttaaaag agcactttga aggccagagt gccctgatct tcacaaacga
    27841 gaatcctttc aggctcttta aactgcttga gcagaccaag acgaagatgg ctgcaaagcc
    27901 cggcgagatc gcgcccgagg atatcgttgt cccgaagggc cctaccacct tcaaaccagg
    27961 tcctatcgtt ggcgagttcc agcaggtggg catacccgcg gtcatcgagg gcggcaaggt
    28021 caagattcgc gagacgaaga cggtcgtgaa gaagggtgag gtaatcaacc ggaaggtcgc
    28081 tgaggtgctc tcgaaactcg ggatcaaacc gatggatgtc gggcttgccc ttcaggctgc
    28141 gtactaccgc ggcagcgtct tcacgccgga tatgcttgca atcgatgagg aggcctacgc
    28201 gaacaacatc gcgttcgcgg ccaggcaggc gttcaacctc tcggtcaacg ctgcaatccc
    28261 gaccaggctt acgatcgagg ccatcattgg ccgggcggta aatgaggcgc ggaacgtcgg
    28321 tgttgaggca ggcgtctacg agaaggatat catcgacctg atcatcgggc gtgccaggcg
    28381 cgagatgctg gctgtggcgc tcgcggcgca ggccaggggg ttcgaccttg atgaggagac
    28441 cctgaaggcg gcaacggccg ccgcacctgc cgtgcccgct gcggctccgg cttctgctgc
    28501 agcacctgcc gcagaggagg agaagaagga agaggagaag aaggaggagg acgaggagag
    28561 cggtcttgcc ggtctcggtg ccctcttcgg ctgatatttc aaaaacgagg tgaatcaaga
    28621 atggagtata tctacgctgc actgctcctg cacaacgcag gcaaggaaat tactgaagag
    28681 aatgtgactg ctgtcttaaa atctgccggt gtcgaggttc aggacgcccg cgtgaaggcg
    28741 ctcgtcgccg cacttgagga tgtgaacatc gaggaggcca tcaacagggc cgcccttgca
    28801 cctgtcgctg ctgctgcacc cgctgccgcc gctgcccctg caggtgccgc cgcagaggaa
    28861 gtggagaagg aagagtcaaa gaaggaagag gacgaggaga gcggtcttgc cggtctcggt
    28921 gccctcttcg gctaaacttt tttaaaattg ctctggatat cgggcgctgt tcttgttgtc
    28981 agatctcttc tttgagggcc agagaaagcc ctccgcaccc gggttatggc agggcgaacc
    29041 cgcagaactg ctcgaacctt gcgaaacttc aatgaccgcg atcccggtcg tccgtgagtc
    29101 cagcagcgca gggggcggtc a
//
LOCUS       DAOSZX010000009        27083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1465_length_27083_cov_21.930960, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000009 DAOSZX010000000
VERSION     DAOSZX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_27083_cov_21.930960"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="mcrG"
                     /locus_tag="PK196_01655"
     CDS             complement(<1..358)
                     /gene="mcrG"
                     /locus_tag="PK196_01655"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050524 - coenzyme-B
                     sulfoethylthiotransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HON40162.1"
                     /translation="MAYKPQYGPGTSKVAANRRKQMNPTQKLEKMRDVTDEDIVLLLG
                     HRAPGAAYPTAHPPLAEQQEPDCPVRKIVTPTDGAKAGDRVRYIQFTDSMFFAPCHPY
                     QRTYTECYRFRGIDPGT"
     gene            complement(372..995)
                     /gene="mcrC"
                     /locus_tag="PK196_01660"
     CDS             complement(372..995)
                     /gene="mcrC"
                     /locus_tag="PK196_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HON40163.1"
                     /translation="MPIGRVTQVVDCRESMGMGKGGGLAQRGTISETRSPDVIVVGMS
                     PGRRHVTKPVCDITSGLRREGAEFSVTTLVLNAGSGVPADSPVAGHVLGAYFGLTEKE
                     VAQMEQHKVAILHHGNVRSHVVQKVRFILEHANIRAIVVSQAPIDFEDLAKEGVRTAV
                     VMPPPDRIRTKGIVMDIVSGVTRGQTPEREKLAEVIRAVMKVLKSPT"
     gene            complement(997..1473)
                     /gene="mcrD"
                     /locus_tag="PK196_01665"
     CDS             complement(997..1473)
                     /gene="mcrD"
                     /locus_tag="PK196_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HON40164.1"
                     /translation="MTEAIYPQVRIVSARFLRPETAEKLLNRLVQVGGIRRMSINGPS
                     LPATVPYGPARGQPNPHPDRKVIRVGDQDVQLEVQVGTIILELEDESYIPAIGEAVDE
                     VFADKDFSCTIQQGRFMKTRPTVSDYAKYGPDADTKILGLVDPRKKVGPVIIQGNK"
     gene            complement(1491..2795)
                     /gene="mcrB"
                     /locus_tag="PK196_01670"
     CDS             complement(1491..2795)
                     /gene="mcrB"
                     /locus_tag="PK196_01670"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HON40165.1"
                     /translation="MAKYKETIDLYDDTGKLLKSGVPLEKISPVINPATRKIIDLTKR
                     TVAVNLAGIQDGLKVGKVAKGHVLGRELNLDIVGNKDAVVSKIKEMVQVAEGDDTNIR
                     EFNGGKLILVEVPKVRLEAASTYDVAVTTVAAATTLAIVDQFNVDPFDAAMVKAAVWG
                     SYPHTMDLSGANVTSILSIPQNNEGLGYALRNIPVNHVVMMTNKNAMQGAALSSTFEQ
                     AGMFEMGNAVGPFERAQLLTYAYQGLNANNLVYNLVKANGATGTIGTVVQSLVERAIE
                     DRVITPGKKGGYFQFYDTKDPMLWNAYTAAGTMAATMVNCGAGRFAQAVSSTLLYFND
                     LVEHETGLPGCDYGRVMGTAVGFSFFSHSIYGGGGPGVFNGNHVVTRHAAGVAIPCVV
                     AAMSLDAGTQMFSPESTSKIYADTYGKVDVFNKPIQQIAQGV"
     assembly_gap    3091..3190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3191..3889)
                     /locus_tag="PK196_01675"
     CDS             complement(<3191..3889)
                     /locus_tag="PK196_01675"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HON40166.1"
                     /translation="METVTLTLKNQPDLYLEADNITPDAFAGKKAEEIADLHVYVGRE
                     QHRLGDFFEVAGKAGSTPETTKIVVNGDLSRVKYIGMKMTGGEIVVNGNADMYVGAWM
                     QGGRITVNGNVDAFAGTGMKGGELVINGNAGNYLGAAYRGDWRGMSGGKIIVAGNAGS
                     DIGTFMTGGEIIIGGDVDVHVATHAEGGRIVIKGNAKSRLGGQMVEGEIYVFGTIDVM
                     MPGFAYRRDVDIDMD"
     gene            complement(3903..5612)
                     /locus_tag="PK196_01680"
     CDS             complement(3903..5612)
                     /locus_tag="PK196_01680"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HON40167.1"
                     /translation="MAEYLIKNGFVFDPVLGIKGDAVDIAIRDGKIVETTEVKDPKVI
                     DATGKTVMAGGVDVHAHVAGPKVNIGRNFRPEDKLFTYKPRSGIERMQGGFSVPTTIR
                     TGYNYARMGYTTVMEAAMPPLYARHTHEEIRDTPILDQGAYPVFGNNWFVLEYLKNNE
                     IENTAAYVAWLLRATKGYAVKVVNPGGTEAWGWGLNCENIHDPVPYFNITPAQIVKGL
                     IEANEYLGLPHSMHIHANNLGNPGNYTTTLDTFRLSEGIKPRSKFGRAQVMHHTHVQF
                     HSYGGDSWGNFESKAPEIIDYVNSHDNITIDLGAVTLDETTTMTADGPFEHHLTELNH
                     LKWANTDVELETGSGVVPYIYSPNIKVCAIQWCIGLELALLAKDPMRVFLSTDHPNAG
                     PFTRYPRIMKWLMSEEARRQQIDSFKHRDKVIEATNLAGIDRELDLYEIAQMTRAGPA
                     KALGLSQIYGGLAPGLDADVAVYDFNPNEPYAPDDIEKAFSSAEHLFKTGIQVIRDHE
                     IVSNGNKRTLWVNAKVNENPQVMRDVREKFLRYYTVTLNNYEVSGRHYLPNPYVIEVD
                     ATQ"
     gene            complement(5616..6929)
                     /locus_tag="PK196_01685"
     CDS             complement(5616..6929)
                     /locus_tag="PK196_01685"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HON40168.1"
                     /translation="MTRTVTDVICPFCGTLCDDIEVVVSDDGKKLYEVYNACAIGAEK
                     FLHSQAEDRITRPRMRQEDGSWKEITYDEAIDYTARMLVNARKPLMYGWSSTNCEAQA
                     IGSEIGELVGAVVDNTATVCHGTSLIAVQDIGIPSCTLGEIKNRADRILFWGCNPAHA
                     HPRHMSRYSIFPRGFFTGKGHTGRKMIVVDPRRTDTAKIADIHLQVEQGRDYELFDAF
                     RVAFKGEHLPDVVAGIPKEKIYEAAETLKSGRFAIIFFGMGVTQSLGKNHNIDAAIAV
                     TRDLNEYTKAAIMPMRGHYNVTGSGQVWGWLFGFPYAVDLSRGFARYNPGETTANDLL
                     WRDEVDAVLVIASDPGAHFPFRSVRKIYDLPSVAIDPHETPTTEVCRVHVPVAFVGIE
                     VGGCAYRMDNVPIETRKVVEPPDGMMTDEEFLGRVLARIKELQGV"
     gene            complement(6926..7327)
                     /locus_tag="PK196_01690"
     CDS             complement(6926..7327)
                     /locus_tag="PK196_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HON40169.1"
                     /translation="MAIRVNLITGRTIQQGVSMEAGKEKPAYTAACGIIELDPTDFKK
                     LGVFRGTNVRVTSDYGSVVVKAVEATQGPHPGIAYIPMGPWANRVVNPNTYSTGMPTF
                     KGTPVTVEAAKNEPVLNSLELVRKGCRGELA"
     gene            complement(7378..7803)
                     /locus_tag="PK196_01695"
     CDS             complement(7378..7803)
                     /locus_tag="PK196_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HON40170.1"
                     /translation="MADENWKPKILAIICNWCSYAGADLAGGSRIQYPPDIRAIRVMC
                     TGRIDPLFILKAFQDGADGVLVSGCHFGDCHYLEGNYKAAKRMFLLKTVLKNIGFEDK
                     RLRMTFVSASEGAKWATVIEDVVKTIKDLGPSPLKEIAR"
     gene            complement(7804..9825)
                     /locus_tag="PK196_01700"
     CDS             complement(7804..9825)
                     /locus_tag="PK196_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HON40171.1"
                     /translation="MAEVKTKEEPRIGVFVCHCGTNIAGSVDVKAVVEYARKLPNVAV
                     ADDYQYMCSTPGQNKIVDAIKEHNLTGVVVAACSPRLHEPTFRGATKMGGLNPFRFEM
                     ANIREQNSWVHMHQPEEATAKAKDAIRIAVAKAALLEDLVPKSVPVEKAAMVVGGGVG
                     GMQAALDLANAGIKTYLVEKNPTIGGRMSQLDKTFPTLDCSQCILTPKMVDVYRNENI
                     ELLTYTEVEAVEGYIGNFDVTLRKKARGVLTTEEAEARGYVGGGCNGCGDCEGVCPVI
                     KPNPFEVGMAPRKAIYIYHPQVSPLLYTVDFDACVKCGLCVDICGDKKAIDLNATDEF
                     MNVKVGTVILATGYDIFPIEEKTEWGYKRYDNVITSLEFERLICASGPTGGHLVRPSD
                     GETPKRIGFVLCAGSRDNTGVGKPYCSRFCCMYSLKHAHQIIEKIPGAVPYIFYMDIR
                     SFGKMYEEFYYRIQNEGAKFIRGRVANILEDPVTKNLHVNAEDTLLGRPVDVEVDLVV
                     LAAAIQPSAETERTRRLFGVSCSQDGWLLEAHPKLNPCGTTTAGVYLAGVCQGPKDIP
                     DTVAQAEGAASAASIPIHLGEVDLEPYFAQCIEEKCAGCGLCVNLCPYSALALVEKDG
                     RRVMQVTEAKCKGCGTCGGFCPGGAIWMQHFATPQIIAQIDAFLLGGEE"
     gene            complement(9837..10739)
                     /gene="hdrB"
                     /locus_tag="PK196_01705"
     CDS             complement(9837..10739)
                     /gene="hdrB"
                     /locus_tag="PK196_01705"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HON40172.1"
                     /translation="MSENKRQYAFFLGCVAPNRYPGIEAAAIKTSEKVGIELLPLKGA
                     SCCPAPGAFGSIDLNIWYAMAARNLVLAEQMGMDIALICNGCYKSIWEVNHKLKHNDE
                     LRDGVNEVLKEIDMEFKGSIDVWHVAELYYDPKIVGVNKIAESVTRPLTGVKVGVHYG
                     CHLMKPQKDRHFGDTENPMWLDELVAALGAEPVQYRNKMQCCGAGGGVRGYDLTHALD
                     ITNEKMINLQEVGADALTEVCPFCQLQFDRGQVEIQDKFGIAWGLPVLHYNELLGLAQ
                     GMSPDELALDLHAVDVEPFLKKIL"
     gene            complement(10744..11325)
                     /gene="hdrC"
                     /locus_tag="PK196_01710"
     CDS             complement(10744..11325)
                     /gene="hdrC"
                     /locus_tag="PK196_01710"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HON40173.1"
                     /translation="MAVKKDYKDQKLAEKLRDRKYYVSDTNPGFLNEVEKIGQTAAHM
                     CFQCGTCTGSCPSAPRSSYRIRLFMRRAVLGLDEEALTDPDLWLCTTCYTCTDRCPRD
                     LAPTDAIMAMRNIAARRDIIPRNFLQTVQLIYKTGHGVPNNDANRAARKKLGLDAEPE
                     TTHKYPEYLPGVRKILDHYGLKERADRILSEGE"
     gene            complement(11336..12505)
                     /locus_tag="PK196_01715"
     CDS             complement(11336..12505)
                     /locus_tag="PK196_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40174.1"
                     /translation="MALFPKYSKTREGQNVIMEQRLLKAVNNLILNAETCTGCGICVD
                     ACPEEAIVLGPVGATRRGAIDYAEPVDVDPEKCSYCGVCVIMCPFNAMTLKIDGEERL
                     PIVEKEGFPTYDMVTKIDEEKCNRCTICEEVCPRDAINRDVPVFEGADEAGKPRQSAL
                     EIKTTFTVDTEKCDYCGICGALCPSITVVRKPTTPETAKIEGEVKWEEITCDGCMICV
                     EACPKDAITLEREFVSTKLPGKVDIEQDNCCTCTWCVHTCPEEAITVEKIFEGDIEIY
                     PEKCPGGCSTCVEVCPCNALYLPSPLPAKLMKGQIEPNIAINKDFCIFCGACVNACPS
                     EGAIVLKRTAIRMQEKDTDLFKQIKEKLLTTRTSKVKETTPGKVEVKVLKEAEEA"
     gene            complement(12521..12793)
                     /locus_tag="PK196_01720"
     CDS             complement(12521..12793)
                     /locus_tag="PK196_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40175.1"
                     /translation="MAFALHVNMEKCTGCNNCVVACPVDALELYTEGPVAKDKIYKVV
                     NGKAVILDFNAELCAGCGVCVEACPYGVIKLAGPWESRARARKVEA"
     gene            13396..>14253
                     /gene="fhcD"
                     /locus_tag="PK196_01725"
     CDS             13396..>14253
                     /gene="fhcD"
                     /locus_tag="PK196_01725"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HON40176.1"
                     /translation="MELNGVTIDDTYAEGFPTWVARPIITAVTEEWAYKAALEAVGFA
                     TSTIGCPAEAGIDCFVPADETPDGRPGYAILICASKKKLKEQVVERLSECVLTAPTTA
                     VFDGLPDLIDEVPEKIPVKLHFFGDGYEEKREVGGRTVWAIPIMEGEYIGEEEFGIVK
                     GVAGGNFFIMGENQMAALMAAQAAVDAISGVCGVITPFPGGIVASGSKVGSKKYKFMN
                     ASTNEAYCPTLREKVAGSKVPDGVRAIYEIVIDGVDEEAVKSAMAEGIRAATKVPGVK
                     YISAGNFGGS"
     assembly_gap    14254..14353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14354..15199
                     /locus_tag="PK196_01730"
     CDS             <14354..15199
                     /locus_tag="PK196_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile2) 2-agmatinylcytidine synthetase"
                     /protein_id="HON40177.1"
                     /translation="CRARGTIEWDAANRMRAKGAAISCDVEGTTMTMLARLGKSPAKF
                     GAADREIGGQALEGVEVIGEEVVTTWAGIAGAGVGVAACLPQAPGVIRAEYPTEDDLR
                     IGGARVNRVRIVSPLYEKVTIGIDDTDTREEGATWVLALRCAENCKIPGVEYLDMRLV
                     QLNPAVPKKTTNCVGSALNFAVRPGQVDTLLDYVRDFIESGSVSKDTGIAVYRGIAFE
                     KESPYLKRVKTDILTLEDAETEAARMGVRFIDSNRRKGRIGALGAVLWGNKGAEAAGL
                     YGETL"
     gene            15183..16076
                     /locus_tag="PK196_01735"
     CDS             15183..16076
                     /locus_tag="PK196_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HON40178.1"
                     /translation="MERLSDPYTIRYPQIVAVTDETGEEVELIEFFDCIGGAMWVKQH
                     YARSPLVRSVRTVGATTRYILSTGSADLALKGSTFPAGIAAVSVEDDEISITYRGLGG
                     GGVGASICRAGAGGVIRCVSDPAGGGRLAGSTIWLPRRERLIIGIDDTDTAEEGATWT
                     LAHNIARAVEDERSRYLSHTIVQLYPVPYRTKNCVAIACEFATSEPAGLIRCYRDYLE
                     DYTLSDETGMAVWRGFDTSPLEEFGCRVKRGEVSPDDLAAIDERRLSIVMDGRGAIGA
                     VAAIPFATRYEEALALWNGST"
     gene            16058..17029
                     /locus_tag="PK196_01740"
     CDS             16058..17029
                     /locus_tag="PK196_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40179.1"
                     /translation="MEWKHLKARVLETGAVRLSGEPADEYISRSAAGPSAGSPGSIFF
                     TAGGGRRVRAEMDDASPIEVVHRGGGEADLIIDGEVISGRLEPPALHCPRQAYITVSG
                     RCIFRCRYCTVPGLPGRRKTIDEIVRMVKDVSDRIDAIAITSGVASSIEEEEAYILEV
                     VAALRSSGLPIGVSIYPTPRTPARLHALGVAEVKFNLEAATPELFAKMCPGLSWETIR
                     EALRSSVALFGRGRVYSNIIVGLGETDDDLERLMEDLAGSGVIPILRPLTPAASLADR
                     PRPSAERLLSLARVHERILREAGLDPRHALTMCAACTGCDLVPGRDA"
     gene            17026..18270
                     /locus_tag="PK196_01745"
     CDS             17026..18270
                     /locus_tag="PK196_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON40180.1"
                     /translation="MKGAKALASAVSQAADRFYAVPGYPVSEVAALTGAVITTNEKVA
                     LEYALGDSLSGRRAAVIVKHVGLNACADPLVHATAQGLRAGVVIVVGDDIRVTGSDVA
                     GDSRYYGEVAWAPVFEPDGDTLGPAVEAAFEASETFSRVTILRVTPDLLDADVPEPSI
                     QRNDLKGSLADPDLTMAGRAVAADRRTAAMFAWARSSPLNRFHPVIAYPPPADAGVLA
                     SLPEERGRPFLREHRLLAPPDAAMEPQRFSTRGFSRTFCRDCPFHPALRILQERGMQA
                     VCDAGCSILAMNPPYRIGVAAYGLGSSVAVAATGPRVALVGDYALLHSGLNALIDVYE
                     RGLPLLCIVFANRRMGMTGGHPVPEILRYIAWADPVVCAADERTTLRHALVLPEGGPQ
                     TVVIEGVCPEGGRHVAMEYRDL"
     gene            18242..19372
                     /locus_tag="PK196_01750"
     CDS             18242..19372
                     /locus_tag="PK196_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HON40181.1"
                     /translation="MLPWNIEICDVTLRDGEQTPGVSFTCDEKMAIAQSLDEIGVEVI
                     EAGFPVVSRAEKECVATVARSGLDARICCLARALKPDIEAALDCDVDIISIFVAISDL
                     HVRHKYRKPREEVVEDALRMVEFAADHGVQIRLAAEDASRTDPAVLIDMYRRGIERGA
                     DLVSFADTVGCLTPLEIHSAVSRILDAGPFPLCIHCHNDLGFAAANTITAAAAGAFQL
                     HTTVNGIGERAGNAALEQVLVALRFKGGVDRYDLSRLQEISRLVARCSGITPERMHPI
                     VGENAFAHESGIHIAAILCDPATYEYVPPELVGSERRFVLGKHTGRKALEHVAKAYGF
                     ELSDEEASWVLEQVKQKSEGKCCVTREVLCDLLRKAREGGAR"
     gene            19369..20580
                     /locus_tag="PK196_01755"
     CDS             19369..20580
                     /locus_tag="PK196_01755"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HON40182.1"
                     /translation="MSTLSERILGAPAGAYVDRDVDIAFAHDGTGVLTLEALREMGVE
                     RLPRPERLRLIFDHIIPANTGTTATLQAELRAFARVSGIPLSDAGCGICHQVMSEGLV
                     RPGMIVVGADSHTCTAGAFGAFATGVGATDMAAIWASGGTWFRVPETIAVNLSGNLSG
                     AAEPKDVALTYVARLGMEGATYRALEFVGEGVRGISMDGRLTLCNMAVETGAKAGIFY
                     ADDLTLSYLADYGVKASPQVPEECRYEQTLDIDLDDIVPLVAVPHRVDTVREAEEIAG
                     TRLDQVFVGTCTNGRYEDLERFARIVRGKKVAIRTLVFPASRAVLSRAIKTGILADIV
                     DAGCVVGPPGCGPCLGAHAGVLGEGEVCLSTANRNFKNRMGVGGEIYLSSVATAAASA
                     LTGEIAVPEAA"
     gene            20580..21050
                     /locus_tag="PK196_01760"
     CDS             20580..21050
                     /locus_tag="PK196_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HON40183.1"
                     /translation="MKGAGPAVCLGNDIDTDLIIAGRYLRTKDRSVWAEHVFEDLDPA
                     LAARLPGSVIVAGRNMGCGSSREQAVIALREAGVVGVVAESFARIFFRNAINVGLPVI
                     EATVPCIDGARVAFDLEEGWVEVDGKRCPARPLSERMLAILRAGGLAACWRASR"
     gene            21047..21874
                     /locus_tag="PK196_01765"
     CDS             21047..21874
                     /locus_tag="PK196_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40184.1"
                     /translation="MIFPPHCKFVGVANGTPCGRDKAYFLTRYIVRETPGGTEVIEVK
                     TDPEETGLLRTVLSSRVLAAGDEVYHYPEPVNVQDRTFLVTEAMRSGYRCTIFSGYGE
                     QTTFVIDPDLSGFLRIHVYDITPPRPHLSATLTDLERTGVFGDLEVVFEHHLRDIREI
                     GADVYPCRAAGFPRTIDADRLRPGDRVAACMTGRELIKECYGNSVTVADNICPLEAVR
                     AEPFIARCCRSERAGVGLRNGLLGAVVHWGASAWDMVEAVRLVATTWRRGYDRDSGC"
     gene            21852..22820
                     /locus_tag="PK196_01770"
     CDS             21852..22820
                     /locus_tag="PK196_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HON40185.1"
                     /translation="MTGIAVVEGDGIGHEVVPVAREILAAVRPDLEFFEVEVGYGRWE
                     RTGSACDEETIADLKSADAILFGAVTTPPDPGYRSVLLQIRQALDLYANVRPIRGEGV
                     DIVIVRENTEGLYSGIEWTEPDRACTVRVVSRRGSERIARCACSLAAARRHLTIGNKA
                     NVLKSDCLFVDICTREAARAGVPCTARFIDALSLDILMHPERYDVIVTTNIFGDILSD
                     TAAYLVGGLGMLPSGNIGERHALFEPVHGSAPDIAGQGIANPIAAIRSAAMLLAHTGD
                     PGSAAVIEEAIDRVLNAGIRTRDLGGNAGTREFGAAVLREVLRRKA"
     gene            22847..23695
                     /locus_tag="PK196_01775"
     CDS             22847..23695
                     /locus_tag="PK196_01775"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HON40186.1"
                     /translation="MVMVGCHISIAGSIDRAVDRARDAGCDTFQIFSRNPRGWRARDL
                     DPDVVEAFRAAVRSSGLGPVFDHMPYLPNLASPDDAIYEKSVAALADELRRCALLGIP
                     YIVTHLGHHRGAGIEEGQERVIAAINRAFHETGDTGVTLLLENTAGEKNSVGTRIEDL
                     ALIYDGLDDRGEFGICFDTCHAFGAGYDLRTADGIDAVVAEIDERIGVDSLHLIHLND
                     SKGDLGSGLDRHEHIGLGSIGEDGFREILRNPQIRRLPLICETPVDARRDDAGNIAKV
                     RELAGY"
     gene            23717..24583
                     /locus_tag="PK196_01780"
     CDS             23717..24583
                     /locus_tag="PK196_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40187.1"
                     /translation="MKRESGSADADAPPSRWRVRGRRQADLCIVWFCILGAVLVGLTV
                     LALANITITELADPLHLIEVAASSGVLGAILLTFSAGANAVLLLILFGTPLAYVLARA
                     PPSRVKGVVESIVDLPLILPHTVAGLLVYLLFMRRGWLGAPLAEIGLAFEDAYPGTVV
                     AMLFVASPFFVNTMREGFEKVPLHLENVARTLGAGRFTTFRTVTLPLSLRHLFNGAIL
                     AWGRAIGEFAAVVMIAYYPFIISTLIYYSFTTSGIRTSRSIAFVVIVVSLGVFYLLRR
                     LTGYLGRYDDRV"
     gene            24570..25649
                     /locus_tag="PK196_01785"
     CDS             24570..25649
                     /locus_tag="PK196_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON40188.1"
                     /translation="MIAFERVSLRLGSFSLKDVSLSINRGDYYFIIGPSGAGKTVLLE
                     AIAGLHRPDSGRILLRGKEITDLPTERRGIGLVYQDYSLFPNMTVIDNVSYSLRVRGV
                     RRQEARERVAGLLERFGVEHLADRYPGTLSGGEQQRVALARAVAVKPDILLLDEPLSA
                     LDPVTQERFIADLRRLHRDEGLTIVHVSHSRREAHMLATRMAVLIDGRLVDEGDADVV
                     LNAPGSREVASFIGVENILDGVVTANHGGRATVDAGGLRFDAQTDAAVGEAVCLCIRA
                     DDLIIAVDDGRRPDAPNTMTGTVIQIVENGPVAEILVDAGMDMTAVMTRRSVRDLGIA
                     PGVRVSLRVREDAIHVIRSALSARE"
     gene            complement(25618..25887)
                     /locus_tag="PK196_01790"
     CDS             complement(25618..25887)
                     /locus_tag="PK196_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HON40189.1"
                     /translation="MIDPHMDTVTILGLFAGALTTLSFAPQAIKAWRSRSTADLSFAM
                     LLLLLAGVLLWFVYGAARGDLAIIVANGATAVLVAFTLALKAQNG"
     gene            complement(25884..27017)
                     /locus_tag="PK196_01795"
     CDS             complement(25884..27017)
                     /locus_tag="PK196_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HON40190.1"
                     /translation="MAESTGAEERGIRFTLEEVAGAVGDFGTIFPILLGVAIVCPDVN
                     VSHFFLFIAAWYIIAGFYYRLPIPIEPMKAIGAIVIAGGLSGGEIVASGIVVGALFLL
                     LGLLGGMTWLADRIPRSVIRGVQAGLALLLLRTSLDYILNDALFAALSIGIILAFFVA
                     SQRTRIPDISALIVILIGLCVGIVTQGMPPLRLIPLPSLIIPLPSDFIAGTWELAIPQ
                     IPLTLTNAILATSLLTHDLFPKRGVDPDRLSRTIGAMNLISTPLGGFPMCHGAGGLAA
                     MYRFGARTGGANIIAGVFILIFAVAFAPPEVLTLIPLGVFGALLVFVAIELGKHSIKT
                     ESYAVTGAIAVLTLVIGLTFAFIIGMIMAYVLKAEVSSPQAGR"
BASE COUNT         5085 a         8075 c         8174 g         5549 t
ORIGIN      
        1 gtgtacccgg gtcgataccg cggaagcggt agcactcggt gtatgtccgc tggtaggggt
       61 ggcagggcgc gaagaacatc gagtccgtaa actgtatgta acggacgcgg tcgccggcct
      121 ttgcaccatc tgtcggtgtt acaatcttcc tgacggggca gtcgggctcc tgctgctcgg
      181 cgagcggcgg gtgcgctgtc ggataggcgg ctcccggcgc cctgtggcca agaagcagca
      241 cgatgtcctc atcagttaca tcacgcatct tttcaagttt ctgggttggg ttcatctgct
      301 tgcgccggtt tgcggcgacc ttggatgtac ccggtccata ttggggcttg tatgccattg
      361 tttcattcac ctcatgttgg gctttttagc actttcataa cggcacggat aacttctgcc
      421 agtttttccc tctcaggtgt ctggccccgt gtcacgccgc tgacgatatc cataacgatg
      481 cctttcgtcc ttatccggtc gggcgggggc ataaccaccg ctgtcctgac tccctctttt
      541 gcgagatcct cgaagtctat cggagcctgg gagactacta ttgcccggat gttcgcgtgc
      601 tcaaggataa accgtacctt ctgcaccaca tgggagcgga cattcccgtg gtgcaggatg
      661 gcaaccttgt gctgctccat ctgggcaacc tctttctccg tcagcccgaa gtaggctcca
      721 agaacgtgac cggcgacggg cgaatccgcc ggaaccccgc ttcctgcatt caggacgagc
      781 gtggtcacgg agaactctgc cccctctctc cggagaccgg aggttatgtc gcagaccggt
      841 tttgtcacgt gtctgcggcc cggggacatg cctaccacga tcacgtcagg ggacctggtc
      901 tcggagatgg tgccacgctg ggcaagacca ccacctttcc ccattcccat gctctcgcgg
      961 caatcgacaa cctgggtcac tcttccgatc ggcatctcac ttgtttccct gtatgataac
     1021 gggacctact tttttcctcg gatcgaccag tccaaggatc ttggtgtcag catcaggtcc
     1081 atacttcgcg tagtcggaca ccgtcggcct ggtcttcata aaccgccctt gttggatagt
     1141 gcaggagaag tccttatcag caaagacctc gtcgactgct tctcctatgg ccgggatgta
     1201 cgactcgtcc tcgagctcca ggatgattgt tccaacctgc acctcaagct gcacatcctg
     1261 gtcgccgacg cggataacct tacggtccgg gtgggggttg ggctgccccc gtgccgggcc
     1321 gtagggaacg gtagcgggaa ggctcggtcc atttatggac atcctgcgga tcccaccgac
     1381 ctgaacaagc ctgttcagga gtttttccgc ggtttcgggt ctgaggaatc gtgcagacac
     1441 gattcgaacc tgagggtata tggcttcagt catcagcatc cttgagttat ttacacaccc
     1501 tgtgcgatct gctggatcgg cttgttgaag acgtcgacct taccgtaggt gtcagcgtag
     1561 atcttcgagg tgctctccgg cgagaacatc tgggttcccg catcgagcga catcgcggcg
     1621 accacgcacg ggatagccac acctgcggca tgcctggtca cgacgtggtt gccgttgaag
     1681 acaccggggc ctccgccacc gtagatcgag tggctgaaga acgagaaccc gacggcggta
     1741 cccatcacac ggccgtagtc gcagccggga agacccgtct cgtgctcaac caggtcgttg
     1801 aagtagagca gcgtcgacga gaccgcctgt gcgaaccgtc cggcaccaca gttgaccatc
     1861 gtggctgcca tggttcccgc agcggtgtag gcgttccaga gcatcggatc cttcgtgtcg
     1921 tagaactgga agtagccgcc cttcttgccg ggggtgatga ccctgtcctc gatggcgcgc
     1981 tcgaccaggc tctggacaac cgtaccgatc gtcccggttg cgccgtttgc ctttacgagg
     2041 ttgtagacca ggttgttcgc gttcaggccc tgataggcgt aggtgaggag ctgggcacgc
     2101 tcaaacggac cgacggcgtt ccccatctca aacattcctg cctgctcgaa ggtggacgag
     2161 agagctgcac cctgcatcgc gttcttgttg gtcatcatga cgacatggtt gaccgggatg
     2221 ttgcggagtg cgtagccgag accctcgttg ttctggggga tcgacagaat ggacgtgacg
     2281 ttggcgccgg aaaggtccat cgtgtgcggg taggaacccc agactgccgc cttgaccatc
     2341 gctgcgtcga acgggtcgac attgaactgg tcgacgatcg caagggtcgt cgcggcagcg
     2401 accgtggtaa ccgccacatc gtaggtggat gcagcctcca ggcgaacttt gggcacctca
     2461 acaaggatga gtttgccgcc gttgaactcg cggatgttgg tgtcgtcgcc ctccgcgacc
     2521 tggaccatct ccttgatctt ggagaccaca gcgtccttgt tgccgacgat atcgaggttc
     2581 aactcacgcc cgagaacatg cccctttgct acctttccga ccttcaggcc atcctgaatc
     2641 ccggcaaggt tgaccgcaac cgtcctcttg gtgaggtcga tgatcttcct ggtcgccggg
     2701 ttgatcacag ggctgatctt ctccagcggc acaccgctct tcaacaactt gcctgtatca
     2761 tcgtacaggt caatcgtttc tttgtatttt gccataattt tcctccatta ccctctttaa
     2821 gtgcatacaa tccaggaatg tactatgaac cctaatcgca tcgctgactg tcgctgccgg
     2881 tgactactgc acgtcctcat gggacaaaat gataataacc ttctccgacg atataagtgt
     2941 tgctattttg ttcgctattg accatataaa atcaatcttg atcttaaaga actgctggaa
     3001 aaatccggat tgcctgggat ggcttgcgga tccaccatgc gcgctggtaa taatgatcta
     3061 atagtatggt aacacattgt aatatttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn gtccatatcg atatcgacat cccgccggta tgcaaacccc ggcatcatga
     3241 cgtcaatggt gccaaagacg tagatctcgc cttccaccat ctgaccaccg agccggctct
     3301 ttgcattgcc cttgataacg atcctgccgc cctcagcgtg ggtcgccacg tggacatcga
     3361 catctccacc gatgatgatc tcaccaccgg tcatgaaggt gccgatatca cttccagcat
     3421 tacccgcaac aatgatcttc ccgccggaca tgccacgcca gtcaccacgg tatgcggcgc
     3481 caaggtagtt cccggcgttg ccgttgatta cgagttctcc acccttcatg ccggttccgg
     3541 caaaggcatc aacgttcccg tttaccgtga tcctgccgcc ctgcatccag gcccccacgt
     3601 acatgtcggc gttgccgttg acgacgatct cgcctccggt catcttcata ccgatgtact
     3661 tgacccgtga aagatcaccg ttgacaacga tctttgtcgt ctcaggcgtc gaccccgcct
     3721 tgccggcgac ttcaaagaag tccccgagcc ggtgctgttc tctgcccacg tagacatgga
     3781 gatcagcgat ctcttctgcc ttcttccctg caaacgcatc cggagtgatg ttgtccgcct
     3841 cgaggtagag atcgggctgg ttttttaggg tgagtgtaac ggtttccatg cttcttctca
     3901 cctcactgtg tggcgtcaac ctcgatcacg tagggattcg gaaggtagtg gcgccccgaa
     3961 acctcgtaat tgttcagcgt gacggtgtag tagcggagga acttctccct gacgtcccgc
     4021 atcacctgcg gattctcgtt caccttcgcg ttcacccaga gcgtccgctt gttcccgttg
     4081 ctcacgatct catggtcacg gatgacctgg ataccggtct tgaagaggtg ctccgcgctg
     4141 gagaacgcct tctcgatatc atccggtgca tagggttcgt tcgggttgaa gtcgtagacc
     4201 gcaacatccg catcgagacc cggggcaagc ccgccgtaga tctgcgacag tccgagcgcc
     4261 tttgcaggtc cggcccgcgt catctgcgcg atctcataga gatcgagttc gcgatcgatc
     4321 ccggcgaggt tggtcgcctc gatgaccttg tccctgtgct tgaaggagtc aatctgctgt
     4381 ctgcgtgcct cctcactcat gagccacttc atgatccgcg ggtagcgggt gaacggccct
     4441 gcgttcgggt gatcggtgga gaggaagacc cgcattgggt ctttggcgag cagcgcaagt
     4501 tcaaggccta tacaccactg gatggcgcaa accttgatgt tcgggctgta gatgtagggc
     4561 acgacccccg aaccggtctc gagttcgaca tcggtgttcg cccattttaa gtggttcagc
     4621 tcggtgaggt gatgctcgaa cggcccatct gcggtcatgg tggtggtctc atcgagcgtc
     4681 acagcaccca ggtcaatggt gatgttgtcg tgcgagttca cgtaatcgat gatctcgggt
     4741 gccttggact caaagttccc ccaggagtcg ccgccgtagg agtggaactg aacgtgggtg
     4801 tggtgcatca cctgagcgcg accgaacttg ctcctgggct tgataccctc cgagagcctg
     4861 aacgtatcaa gcgtcgtcgt gtagttgcct ggattcccca ggttgtttgc gtgaatatgc
     4921 atcgaatgcg ggagaccgag atactcgttt gcctcgatca ggcccttcac gatctgcgcc
     4981 ggggtgatgt tgaagtaggg aaccgggtca tgaatgttct cacagttcag accccagccc
     5041 caggcctcgg tgccgcccgg gttgacaacc ttgaccgcgt agccctttgt ggcccgcaga
     5101 agccaggcga cgtaggcggc ggtgttctcg atctcgttgt tcttgaggta ctcgagcaca
     5161 aaccagttgt tcccaaagac tggataggca ccctggtcga ggatcggggt gtccctgatc
     5221 tcctcatggg tgtgccgcgc atatagaggc ggcatggccg cctccatcac ggtcgtgtaa
     5281 cccatacggg cgtagttgta gcctgtccgg atggttgtcg ggacagagaa tccaccctgc
     5341 atccgctcga taccgctcct gggcttgtag gtgaagagtt tgtcctctgg acggaagttc
     5401 cggccgatat tcaccttcgg tccggcaacg tgggcatgga catcaacacc ccccgccatg
     5461 acagtcttgc ccgtggcatc gatgaccttc ggatctttga cctccgttgt ctcgacaatc
     5521 ttgccgtccc tgatggcgat atcaaccgca tcccccttga tcccgaggac aggatcaaag
     5581 acaaacccgt tcttgataag atactctgcc atcgcttaaa ccccctgtag ttccttgata
     5641 cgtgcaagta cacgaccgag gaactcctca tcagtcatca taccatccgg cggctcgacg
     5701 accttcctgg tctcgatcgg tacgttgtcc atcctgtatg cacacccgcc gacctcgatg
     5761 cccacaaaag cgaccggaac atgaaccctg cagacctcgg tggtcggggt ctcgtgcgga
     5821 tcgatcgcaa ccgatggaag gtcatagatc ttcctgacag acctgaacgg gaagtgcgcc
     5881 ccgggatcgc tggcgataac cagaacggca tctacctcat ctctccagag caggtcgttt
     5941 gccgtcgtct caccggggtt gtagcgggca aatccgcggg ataggtccac cgcgtagggg
     6001 aacccgaaca gccagcccca gacctgaccg gagccggtga cgttgtagtg cccgcgcatc
     6061 ggcataatgg cagcctttgt gtactcgttt aagtcacggg tgaccgcgat ggccgcatcg
     6121 atgttatggt tctttccaag cgactgggtc acacccatac cgaagaagat gatcgcaaac
     6181 cggccgctct tgagcgtctc ggcggcctcg tagatcttct ctttcggaat tccagcaacc
     6241 acgtccggga ggtgctcacc cttgaaagca actcggaatg cgtcgaagag ctcgtagtcg
     6301 cgtccctgct cgacctgcag gtggatgtct gcaattttcg cggtgtcggt acgccgcggg
     6361 tcgaccacga tcatcttccg gccggtgtgg cccttgcctg tgaagaatcc acgggggaag
     6421 attgagtagc gggacatgtg gcgtggatgg gcgtgggccg ggttgcatcc ccagaagagg
     6481 atccggtcag cacggttctt gatctcgccg agcgtgcagc tcgggatacc gatatcctgg
     6541 accgcaataa gtgacgttcc atgacagaca gtcgccgtgt tgtccacgac cgccccgacc
     6601 aactcgccga tctctgatcc gatagcctgg gcttcgcagt ttgtggaact ccagccgtac
     6661 atcaggggct ttcttgcgtt gacaagcatc ctggcggtgt agtcgattgc ctcatcgtat
     6721 gtgatctctt tccaggaccc atcttcctgc cgcatgcgcg gccgggtgat acggtcctct
     6781 gcctgggagt ggaggaactt ctcggcacct atggcgcagg cgttataaac ctcgtagagt
     6841 ttcttcccat catcgctgac aacgacctct atgtcgtcgc agagcgttcc gcagaacgga
     6901 cagattacgt cagttacagt ccgggtcatg ccagttcacc cctgcacccc ttgcgaacca
     6961 gttcaagcga attcaggacg ggttcattct tggcagcctc cactgtaacg ggggtgcctt
     7021 tgaatgtcgg catcccggtg gagtaggtgt tcgggttgac caccctgttc gcccagggac
     7081 ccatcggaat atatgctatg ccggggtgag gaccctgggt tgcctcgacc gccttcacga
     7141 cgacgctgcc gtagtcgctc gtgacgcgta cgttcgtgcc acggaaaact cccagttttt
     7201 tgaagtctgt cgggtcgagc tcgatgatcc cgcaggcggc ggtgtaggcg ggtttttcct
     7261 tacccgcctc catcgacacc ccctgctgga tggtgcgacc cgtgatcaaa ttcactctga
     7321 ttgccattgg tattcatccc ctgtctgtta ttggtatatt attatctggc aattatctta
     7381 tctggcaatc tccttgagcg ggctcggacc aaggtctttg atggtcttga cgacgtcttc
     7441 gatgaccgtg gcccactttg caccctcgga tgcggagaca aaggtcatcc tgagacgttt
     7501 gtcctcaaaa ccgatgtttt tgaggactgt cttgaggagg aacatcctct tggcggcctt
     7561 gtagttgccc tcaaggtagt ggcagtcacc gaagtgacag ccggagacca gcaccccatc
     7621 cgctccatcc tggaatgcct tcaggatgaa gagaggatcg atccggccgg tgcacataac
     7681 acggatggcg cggatgtctg gcgggtactg aatacgggag ccgcctgcaa ggtccgcacc
     7741 ggcgtaggag caccagttgc agatgatggc aaggatcttt ggcttccagt tctcgtctgc
     7801 catttactct tcacccccaa ggaggaacgc atcgatctgt gcaatgatct ggggtgtagc
     7861 aaagtgctgc atccagattg caccgccggg gcagaacccg ccgcatgtcc cgcagccctt
     7921 gcacttggcc tcggtgacct gcatgaccct gcgcccatcc ttctcgacaa gtgcgagggc
     7981 actgtaaggg cagaggttaa cacacagccc gcatccggcg cacttctcct cgatacactg
     8041 ggcgaagtag ggctcaagat ccacctctcc aaggtgaatc gggatggatg ccgcggatgc
     8101 cgcgccctcg gcctgggcaa ccgtgtccgg tatatccttc ggcccctggc agacaccggc
     8161 gaggtagaca ccggcggttg tggtgccgca ggggttgagc ttcgggtgag cctcgaggag
     8221 ccagccgtcc tgcgaacagg atacgccaaa gagcctccgg gtcctctcag tctctgcgct
     8281 gggctggatt gcggccgcaa gcacaaccag atcgacttca acgtccaccg gccggccaag
     8341 gagcgtgtct tcagcgttga cgtggaggtt ctttgtgacc gggtcttcga ggatgtttgc
     8401 aaccctgccg cggatgaact ttgcgccctc attctggatg cggtagtaaa actcctcgta
     8461 catcttgccg aatgaacgga tgtccatgta gaagatgtag ggcaccgcgc ccgggatctt
     8521 ctcgatgatc tggtgggcgt gcttgaggga gtacatgcag cagaaccgcg agcagtaggg
     8581 cttaccgacg ccggtgttgt cgcgagaccc tgcacatagc acgaacccga tcctctttgg
     8641 agtctcgccg tcgcttgggc ggacgaggtg gccgccggtc gggccggaag cgcagatcaa
     8701 ccgctcgaac tcaaggcttg tgatgacgtt gtcatacctc ttgtagcccc actcggtctt
     8761 ctcctcgatc gggaagatgt cgtagcctgt tgcaaggatg acggtaccga ccttgacgtt
     8821 catgaactcg tccgttgcat taaggtcaat cgccttctta tcgccgcaga tatcgacaca
     8881 gagaccgcac ttgacacagg catcgaaatc gacggtgtaa agcaaggggg agacctgcgg
     8941 gtggtagatg tagatcgctt tacgcggggc catgccaacc tcgaaggggt ttggcttgat
     9001 gacagggcag acaccctcgc agtcgccgca gccgttgcag ccgccgccaa cgtagccgcg
     9061 ggcttccgcc tcttccgttg taaggacacc gcgcgccttc ttccggaggg tcacgtcgaa
     9121 gttgccgatg taaccctcga ccgcctcaac ctcggtgtag gtcaggagtt ctatgttctc
     9181 gttgcggtag acatccacca tcttcggggt caggatgcac tgggaacagt ccagcgtcgg
     9241 gaaggtcttg tcgagctggg acatcctgcc gccgatggtg gggttcttct ccacaaggta
     9301 ggtcttgatg cctgcgtttg caaggtcaag cgccgcctgc ataccgccga caccgccgcc
     9361 gacgaccatc gcggccttct caaccgggac gctctttgga acgaggtcct cgagaagggc
     9421 cgctttcgct acggcgatcc tgatcgcgtc ctttgccttc gcggtggcct cttctggctg
     9481 atgcatgtgc acccaggagt tctgctcgcg gatgtttgcc atctcaaacc ggaaggggtt
     9541 caagccaccc atcttggttg ctccacggaa cgtgggctcg tgcagacggg gtgaacaggc
     9601 cgccacgact accccggtaa gattatgctc ttttatggca tctacgatct tgttctggcc
     9661 gggagtcgag cacatgtact ggtaatcgtc tgctacggca acgttcggga gtttcctggc
     9721 gtattccacg accgccttga cgtcgacgga acccgcaata ttggtaccac agtggcacac
     9781 aaaaacgcca attcttggct cttctttcgt tttgacttct gccatgtatc tgcacctcac
     9841 aggatcttct tcaggaacgg ctcaacatcg acggcatgta ggtcgagtgc cagttcatcc
     9901 gggctcatgc cctgcgcaag cccaaggagc tcgttgtagt gcagaaccgg gagcccccag
     9961 gcaattccaa acttatcctg gatctcgacc tgacccctgt caaactggag ctggcagaac
    10021 ggacagactt cggtcaacgc atctgcgcca acttcctgca ggttaatcat cttctcgttc
    10081 gtgatgtcga gtgcgtgtgt caggtcatag ccgcggacac cgccaccggc accgcagcac
    10141 tgcatcttgt tgcggtactg gaccggctcg gcacctagcg cagcgaccaa ttcatccagc
    10201 cacatcgggt tctcggtgtc gccgaagtgc cgatctttct ggggcttcat caggtggcag
    10261 ccgtagtgga cgccgacttt tacacccgtc agagggcggg tgacgctctc ggcgatctta
    10321 ttgacaccca cgatcttggg gtcatagtac aactctgcaa cgtgccagac gtcgatagag
    10381 cctttgaact ccatatcgat ctctttgagc acctcgttga ccccgtcacg gagttcgtcg
    10441 ttgtgcttca gcttgtggtt aacttcccag atcgacttgt agcatccgtt gcagatgagg
    10501 gcgatatcca tgcccatctg ctcggcgagc accagattcc gggccgccat cgcgtaccag
    10561 atgtttaggt cgatcgaacc gaacgcaccc ggcgccgggc agcagcttgc gcccttcagg
    10621 gggaggagtt cgatgccgac cttctcactg gtctttatgg ccgccgcctc gatgccgggg
    10681 tagcggttcg gggcgacaca cccgaggaag aatgcatact gacgcttgtt ctcgctcatg
    10741 ttatcactca ccctccgaca gtatccggtc ggcacgttct ttcagcccgt agtggtccag
    10801 gatcttcctg acgcctggaa ggtactctgg gtatttgtgc gttgtctccg gttccgcgtc
    10861 gagaccgagc ttcttacggg cagcacggtt tgcatcattg ttcgggacgc cgtgccccgt
    10921 cttgtagatg agctgtacgg tctggaggaa gttgcgcgga atgatatcgc gacgggcggc
    10981 aatgttcctc atcgccatga tagcgtcggt cggcgcaaga tcacgtggac accggtcggt
    11041 gcatgtgtag caggttgtgc agagccagag gtccgggtcg gtcagggcct cctcgtcgag
    11101 accgaggact gccctgcgca tgaacagccg gatacgatag gaactccgcg gtgccgaggg
    11161 gcacgatccg gtgcaggtgc cgcactggaa gcacatgtgg gcggccgtct gcccgatctt
    11221 ctcaacctca ttgagaaacc cgggattggt gtcagaaacg tagtacttgc ggtcccggag
    11281 tttctcagcg agtttctggt ctttataatc tttcttcact gccattgact atccctcacg
    11341 cctcttccgc ctctttcaga accttgacct caacctttcc aggggtggtc tctttaacct
    11401 ttgatgtcct ggtggtgagc agtttctcct tgatctgctt gaagagatcc gtatccttct
    11461 cctgcatccg gatggccgtc cgcttcagga caattgcacc ctcgctcggg caggcattga
    11521 cgcatgcgcc gcagaagata cagaagtcct tgttgatggc tatattgggc tcaatctgcc
    11581 ccttcatcaa ctttgccggg agagggctgg gcaggtaaag cgcattgcag gggcatacct
    11641 caacacaggt cgaacagcca cctgggcact tctcggggta gatctcgata tccccttcaa
    11701 agatcttctc aacggtgatc gcctcttccg gacaggtgtg tacgcaccag gtgcaggtgc
    11761 agcagttgtc ctgctcgata tcgaccttgc cgggcagttt ggtgctgacg aactcgcgct
    11821 ccagggtgat tgcatccttg gggcaggcct cgacacagat catacagccg tcgcaggtaa
    11881 tctcttccca ctttacctcg ccttcgatct tggcggtctc gggggtcgtg ggtttacgaa
    11941 caacggtgat actcggacag agcgcaccgc agatcccgca gtaatcgcac ttctcggtat
    12001 cgacggtgaa cgtcgtcttg atctcaagtg ctgactggcg gggcttgccg gcttcgtcag
    12061 cgccctcgaa gacggggacg tcccggttga tggcatcccg cgggcagacc tcttcacaga
    12121 tggtgcagcg gttgcacttc tcttcgtcga tcttggtgac catgtcgtat gtggggaatc
    12181 cctccttctc gacgatcggg agcctctcct ctccatcgat cttgagcgtc atcgcattga
    12241 aggggcacat tatgacacag acgccacagt aggaacactt ctcgggatcg acgtctacag
    12301 gttcagcata gtcgatcgca ccacggcgtg ttgcaccaac cgggccgagc acgattgcct
    12361 cttccggaca ggcgtcgaca cagatgccgc agcccgtaca ggtctcagcg ttcaggatca
    12421 ggttgttgac cgctttcagg agcctctgct ccataatgac gttctgtcct tcccgcgttt
    12481 tggaatactt tggaaacagt gccatactct tgtatcactc ctaggcttcc acttttcggg
    12541 ctctcgcccg gctttcccag ggtcctgcaa gttttataac tccgtagggg caggcctcga
    12601 cgcaaacgcc gcaaccggcg cagagctccg cattgaaatc aaggataacg gccttgccgt
    12661 ttacgacctt gtagatctta tccttggcta ccggaccttc ggtatagagc tccagagcat
    12721 cgacgggaca tgccaccacg cagttgttgc agccagtaca tttctccata ttgacgtgca
    12781 atgcaaatgc catggtttca cgcaccagat ctgatcgatt aaaaatcgat atgaaaagaa
    12841 ttgtcaaaga atatacataa actttctgaa attcggcagc cttatattcg ggcagcactg
    12901 ttattcacag gattttataa atcaaacaga ttgccatgtt gaccaattcc acctggtctc
    12961 accattaaac ccgtttccgg cacaggagaa gagaaataaa acataaatat cagcctggtg
    13021 ctgctggtga aaattggtgg gtaaacgccg atgttaacaa cgatgccgga gaacacgcca
    13081 tcccgtggcg gtggagggac tgtcaattcc ccacctcccc tggggggtga gatgcgccgc
    13141 gcggtgagag cagcatccat ttcccgtacc cgtcgattct tcgcccccag gaacaaggtt
    13201 gcacagcaac cccgccctta aaaacccggc cccctgcgcc catactgctc tactgtgtcc
    13261 gggtgtacct gctgcgcagg agtgtgcaac ccggatcgcc ccattttgcc cttctccgtg
    13321 gtgctgcatc gtcacataaa aaaagaaacc ttattctgtc catatcgtaa cattgtagca
    13381 aacgaggtgt aatttatgga actgaatggc gttactatag acgataccta tgcggagggt
    13441 ttccctacct gggtggcacg gccgatcatc accgccgtca cggaagagtg ggcttacaag
    13501 gccgctctgg aggcggtagg gtttgccacc tcgaccatag ggtgccctgc cgaggcgggt
    13561 atcgactgct ttgtccccgc tgatgagacc cctgacggac gacccggcta tgcgatcctg
    13621 atctgtgcaa gcaagaagaa actcaaagag caggtcgttg agcgcctatc tgagtgtgtt
    13681 ctcaccgcac cgacgacagc ggtctttgac ggccttccgg atctcattga cgaggttccg
    13741 gagaagatcc cggtcaagct ccacttcttc ggcgacggat acgaagagaa gcgcgaggtt
    13801 ggcggcagaa cggtatgggc cataccgata atggaggggg agtatatcgg tgaggaggag
    13861 ttcggtatcg taaaaggtgt tgcaggcggc aacttcttca tcatgggcga gaaccagatg
    13921 gccgccctca tggccgcaca ggcagcggtc gacgccatca gtggtgtctg cggagtcatc
    13981 acacctttcc ccggcggcat cgttgcgagt ggttcgaagg ttgggagcaa gaagtacaaa
    14041 ttcatgaacg caagcacgaa cgaggcctac tgcccgacgc tccgcgagaa agttgcagga
    14101 agcaaggttc ctgatggcgt cagggcaatc tacgagatag tcatcgacgg tgtcgacgag
    14161 gaggcggtaa agagcgctat ggcagagggt atccgcgccg caacgaaggt gccgggcgtg
    14221 aagtacatca gtgccgggaa cttcggcggc agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnntgccggg ccagaggaac tatcgagtgg gatgcggcga accggatgcg
    14401 ggcaaaaggt gctgccatct cctgtgatgt tgaagggacg acgatgacga tgctcgcccg
    14461 cctgggcaag tccccggcga agttcggggc cgccgaccgg gagatcggcg gccaggcact
    14521 tgagggtgtc gaggttatcg gggaggaggt ggtgaccacc tgggccggga tcgccggtgc
    14581 gggagtcggg gtcgcggcct gcctgccgca ggcgcccggt gtgatccggg ctgaataccc
    14641 caccgaggac gacctcagga tcggcggcgc cagggtcaac cgcgttcgga tcgtatcgcc
    14701 cctctacgag aaggtgacga tagggatcga tgacaccgac acccgtgagg agggggccac
    14761 gtgggtcctt gcactccgct gcgccgagaa ctgcaaaatc ccgggggtcg agtatcttga
    14821 catgcgcctc gtgcagctca accctgcagt cccgaagaag accaccaact gtgtcgggtc
    14881 ggccctgaac ttcgctgtcc gccccgggca ggtggacacg ctccttgact acgtccgcga
    14941 cttcattgag tcggggagtg tcagcaaaga caccggcatc gccgtgtacc gcgggatcgc
    15001 gtttgagaag gagtccccat acctcaagag ggtcaagacc gatatcctga cactcgaaga
    15061 tgcagagacc gaggcggcgc gtatgggtgt caggtttatc gactcgaaca ggcggaaagg
    15121 ccggattggc gcactgggcg cagtcctctg gggcaacaaa ggtgcggaag cggcaggact
    15181 gtatggagag actctctgat ccgtacacga tacggtatcc acagatagtg gcggtgaccg
    15241 acgagaccgg tgaagaggtt gaactcatcg agttcttcga ctgcatcggc ggtgcgatgt
    15301 gggtgaaaca gcactacgcc aggagtccgc tcgtccgctc cgtccgcacc gtgggagcga
    15361 ccacacgcta catcctctca acggggagcg ccgatctcgc cctcaaaggt tcgaccttcc
    15421 cggccggtat tgccgccgtc agcgttgaag acgatgagat ctccatcacc taccgcggcc
    15481 tcggcggagg gggcgtgggg gcatccatct gccgcgccgg agcaggcggg gtcatccgct
    15541 gcgtgagcga ccctgccggc ggcggccgcc ttgcgggttc gaccatctgg ctcccccggc
    15601 gcgaacggct gatcataggt atagacgaca ccgacaccgc tgaagagggg gccacctgga
    15661 cgctggccca caacatcgcc cgtgccgtgg aggacgagcg ttcccgctac ctctcacaca
    15721 ccatagtcca gctctaccct gtcccctacc ggaccaagaa ttgtgtcgct atcgcctgcg
    15781 agtttgcaac ctccgagccc gccggactca tcaggtgtta ccgcgattac ctggaagact
    15841 acaccctctc cgatgagacc ggaatggcgg tctggcgcgg gtttgacact tcaccgcttg
    15901 aggagttcgg ttgccgggta aaacgcggcg aggtctctcc cgatgacctt gccgccatcg
    15961 atgagaggcg cctctcaatc gtcatggacg gccggggggc gatcggggcg gtggcggcga
    16021 tcccgtttgc cacacgatac gaggaggcgc tggcgctatg gaatggaagc acctgaaagc
    16081 ccgggtgctc gagacgggcg ccgtccgcct ctccggcgaa cccgcggatg aatacatctc
    16141 ccgctccgct gccggccctt cggcagggtc gccagggtcg atcttcttta cagccggcgg
    16201 tggccggagg gtaagggccg aaatggatga tgcaagcccc atcgaggttg ttcaccgtgg
    16261 gggcggggag gccgacctca tcatagacgg cgaggtcatc tccggccggc tcgaaccccc
    16321 tgccctccac tgcccccgcc aggcctacat caccgtcagc gggaggtgta tattccgttg
    16381 ccgctactgc acggtgccgg gtctcccggg acggcgaaag accatcgatg agatcgtccg
    16441 gatggtaaaa gatgtctcgg accggatcga cgccatcgcc atcacaagcg gcgtcgcctc
    16501 ctctatcgag gaggaggagg cctacatcct ggaggtcgtc gcggcgctcc gctcgtctgg
    16561 cctccccatc ggggtctcga tctacccgac cccgagaacc ccggcacgcc tccatgccct
    16621 gggcgtcgcc gaggtgaagt tcaacctgga ggcggcaacc cccgaactct ttgcgaagat
    16681 gtgcccgggg ctctcatggg agacgatccg ggaggcgctc agatcttctg tcgcgctttt
    16741 cgggcgcggc agggtctact cgaacatcat cgtcgggctc ggcgagaccg acgacgacct
    16801 ggagcggttg atggaggacc tcgccgggag cggggtcatc ccgatcctcc ggccgctcac
    16861 cccggcggca tcgcttgccg accggccccg cccatccgcc gagcggctgc tctcgcttgc
    16921 ccgcgttcac gagaggatcc tccgggaggc cgggctcgac ccccgccatg ctctaaccat
    16981 gtgcgcggcg tgcactgggt gcgaccttgt gccggggagg gatgcatgaa gggtgcaaaa
    17041 gcgcttgcca gcgctgtatc tcaggccgcc gaccggtttt acgccgtccc cggctacccg
    17101 gtatctgagg ttgccgccct gacgggggcg gtcatcacaa ccaacgagaa ggtggcgctc
    17161 gagtacgccc tgggcgactc gctctccggg aggagggctg ctgtgatcgt caaacacgtc
    17221 gggctcaatg cctgcgccga cccgctggtg cacgcaaccg cccaggggct ccgggcaggt
    17281 gtcgtgatcg tcgtcggcga cgatatccgt gtcaccggat cggacgtcgc tggcgactcc
    17341 cggtattacg gggaggtggc gtgggctcct gtgttcgaac ccgacggcga tactctagga
    17401 cctgccgtgg aggcggcgtt tgaggcatcg gagaccttct ccagggtgac catactcagg
    17461 gtcacccccg atcttctgga cgcagacgtc ccagagccct ccatccagcg gaatgacctg
    17521 aaggggagcc tggctgaccc tgatctcacc atggccggcc gggcagtggc cgctgaccgg
    17581 cggactgccg cgatgtttgc ctgggcccgg tcgtcgccgc tgaacaggtt ccaccctgtc
    17641 atcgcctacc cgccgccggc ggacgcaggg gtgctcgcct cactccccga agagaggggt
    17701 cgccccttcc tccgggagca ccgtctcctc gcccctccag acgccgccat ggagccgcag
    17761 cggttctcca cacgcggttt ctcgcgaact ttctgcaggg actgtccgtt tcatccggcc
    17821 ctccggatcc ttcaagagcg cgggatgcag gcggtctgcg acgccggatg cagcatcctg
    17881 gcgatgaacc cgccctaccg catcggtgtt gccgcgtatg gtctcggttc gtcggtagcc
    17941 gtcgcggcga ccgggccgcg tgtcgccctc gtcggggact acgcgctcct gcattcaggg
    18001 ctaaacgccc tcatcgacgt ctacgaacgt ggacttccgc tcctctgtat tgtctttgcg
    18061 aacagacgga tggggatgac cggcggtcac cccgtccccg agatccttcg ctacatcgcc
    18121 tgggcagacc cggttgtctg cgctgccgac gagcgcacga cgcttcgcca tgcgctcgtc
    18181 ctcccggagg gcggcccaca aacggtggtg atcgaaggtg tctgcccgga aggtggacga
    18241 catgttgcca tggaatatcg agatctgtga tgtgacgctg cgagacgggg aacaaacccc
    18301 tggtgtctcg tttacctgtg atgagaagat ggcgatcgcg caatcgctcg acgagatcgg
    18361 cgtggaggtg atcgaggccg ggtttcccgt ggtctccagg gccgagaaag aatgtgtcgc
    18421 gaccgtcgcc agaagcggcc tggatgccag gatctgctgc ctggcacggg cgctcaaacc
    18481 cgatatcgag gcggcgcttg actgtgacgt ggatataatc agcatcttcg tcgcgatctc
    18541 cgacctccat gttcggcaca agtaccgcaa accgcgggag gaggtggtcg aggacgccct
    18601 ccgtatggtg gagttcgccg ccgaccacgg cgtacagatc cggcttgccg ccgaggacgc
    18661 ctcacggaca gacccggcgg ttctcatcga tatgtaccgc agggggatcg agcgcggcgc
    18721 cgaccttgtc agtttcgccg ataccgtcgg ctgtctcacc cccctcgaga tccattccgc
    18781 tgtctccagg atcctggatg ccgggccctt cccactctgc atccactgcc acaacgacct
    18841 cgggtttgca gctgcaaaca ccatcaccgc cgcggccgcc ggagcgttcc agctccacac
    18901 caccgtgaac ggcataggcg aacgtgccgg gaacgcagcg ctcgaacagg tgctcgttgc
    18961 cctgcgcttt aaaggcgggg ttgatcgcta cgacctctcc cggttgcagg agatctccag
    19021 gctggttgcc cgatgctctg gcataacccc ggagcggatg catcccatcg tcggcgagaa
    19081 cgccttcgcc cacgagagcg ggatccacat cgccgccatc ctgtgcgatc cggcgaccta
    19141 cgagtatgtc ccccccgaac tggttggcag cgaacgccgg ttcgtgctcg ggaagcatac
    19201 cgggagaaag gcgctcgaac acgttgcaaa ggcttacggc ttcgaactct ccgacgagga
    19261 ggcgagctgg gttctcgagc aggtcaagca gaagagtgag ggaaaatgct gtgtcacacg
    19321 cgaggttctc tgcgacctcc tgcggaaggc aagggagggg ggcgcccggt gagcacgcta
    19381 tcagagcgga tactgggcgc accggcgggg gcatacgtcg accgcgatgt cgatatagcc
    19441 ttcgcccacg acggcaccgg cgttctcacg ctggaagcgc tccgcgagat gggggtggaa
    19501 cgactccctc gccccgagcg tctccgcctg atcttcgacc acatcatccc ggcaaacacc
    19561 ggcacaacag cgacacttca ggccgaactt cgggcgtttg ccagggtttc cgggatacca
    19621 ctctcggatg ccggatgcgg gatctgccac caggtgatga gcgaaggtct cgtccggccg
    19681 gggatgatcg tcgtcggggc cgactcccac acctgcaccg ctggcgcatt tggagcgttc
    19741 gccacagggg ttggcgcgac cgatatggcg gcgatctggg cgtcgggcgg cacctggttc
    19801 cgtgtcccag agacgatcgc ggtcaacctc tccggcaatc tctccggcgc cgcggagcca
    19861 aaagacgtcg cactcaccta cgtcgcccgc ctgggaatgg agggcgcgac ctaccgggcg
    19921 ctggagttcg tcggcgaggg ggttagaggg atctctatgg acgggcggtt gaccctctgc
    19981 aacatggcgg tcgagacggg ggcaaaggcc gggatcttct acgccgacga tctcacactc
    20041 agttaccttg cggactacgg ggttaaagcc tcaccgcagg tgccggagga atgccgctac
    20101 gaacagactc tcgatatcga tctcgatgat atcgtccctc tcgtcgccgt cccccaccgg
    20161 gtggacaccg tccgggaggc cgaagagatc gccgggacac gcctcgacca ggtcttcgtc
    20221 ggtacatgca caaacggccg ctacgaggat ctggagcggt tcgcccgtat tgtccgcgga
    20281 aagaaggtgg ccatccgcac cctggtcttc cccgcatcgc gtgcggtgct ctcccgggcg
    20341 atcaagaccg gcatcctcgc tgacatagtg gatgctggat gtgtggtcgg accgccgggg
    20401 tgcgggccgt gcctcggggc gcatgccggg gtgctggggg agggggaggt ctgtctctcc
    20461 acggccaaca ggaacttcaa gaaccggatg ggcgtgggcg gcgagatcta cctctcctcg
    20521 gtcgccacgg ccgccgcaag cgctctcacc ggggagattg ccgtcccgga ggcggcatga
    20581 tgaagggagc agggcccgcg gtctgcctcg ggaacgatat cgacaccgac ctcatcatcg
    20641 cgggccgcta cctccggacg aaagaccgtt cggtctgggc ggaacatgtc tttgaggacc
    20701 ttgaccctgc gcttgcagcc cgtctccccg gttcggtcat cgtcgccggg cggaacatgg
    20761 gctgcggctc gtccagggag caggcggtga tcgccctccg cgaggccggg gtggtcgggg
    20821 tcgtagcgga atcgtttgcc cgcatattct ttcgcaacgc catcaacgtc ggtctgccgg
    20881 tgatcgaggc cacggttccc tgcatcgatg gtgcccgcgt cgccttcgat ctggaagagg
    20941 ggtgggtgga ggtggacggc aaacgctgcc ccgcccgccc cctctcagag aggatgcttg
    21001 ccatcctacg ggccggcggg cttgcagcct gctggagggc gtcccgatga tatttccacc
    21061 acactgcaag tttgtcgggg ttgcaaacgg caccccctgc gggagggata aggcctactt
    21121 cctcacacgc tacatagtcc gggagacccc ggggggcacg gaggtcatcg aggttaaaac
    21181 agatccggaa gagaccggac tcctccggac ggtgctctcc agccgtgtgc ttgccgccgg
    21241 agatgaggtc taccactacc ccgagcccgt gaacgtccag gacagaactt tcctggtgac
    21301 cgaggccatg aggtcaggct accggtgtac catattctca ggctacggcg agcagaccac
    21361 gtttgtcatc gaccccgacc tctcgggttt ccttcgcatc cacgtctacg acataacacc
    21421 gccccggcca cacctctcgg ccacgctcac cgacctcgag agaacagggg tcttcggcga
    21481 cctcgaggtg gtctttgagc accatctccg tgacatcagg gagatcggtg ccgatgtcta
    21541 cccctgccgc gcggccgggt tcccgcgcac aattgatgcc gacaggctcc gccccggcga
    21601 ccgcgtcgca gcgtgcatga cagggcgtga actgataaag gagtgttacg gcaactctgt
    21661 taccgtggcg gataacatct gcccgctcga ggctgtgagg gccgaaccct tcatcgcccg
    21721 gtgctgcagg agcgagcggg cgggtgtcgg cctccggaac ggccttctcg gtgcggttgt
    21781 ccactggggt gcgtcggcat gggatatggt tgaggccgtg agactggttg caaccacatg
    21841 gaggagaggg tatgacaggg atagcggttg ttgagggcga cggtataggg cacgaggtcg
    21901 tcccggtcgc ccgcgagatc ctcgcagccg tgcgcccgga tctcgagttc tttgaggtcg
    21961 aggtgggata cggccgctgg gagcggacag ggagcgcctg tgacgaggag acgatcgccg
    22021 acctgaagtc ggcggacgca atcctctttg gcgccgtcac aacgccgccc gatccggggt
    22081 acagaagcgt tcttctgcag atccgacagg ctctcgacct atacgcaaac gtccgcccta
    22141 tacgtggtga aggggtcgac atcgtcatcg tgcgggagaa taccgagggg ctttactcgg
    22201 gcatcgagtg gaccgaaccc gatcgtgcct gcaccgtccg ggtcgtatcg cgcaggggaa
    22261 gcgagcggat tgcccgctgc gcctgcagtc ttgctgcggc ccgccgccac ctcaccatcg
    22321 ggaacaaggc aaacgtcctg aagtcggact gtctgtttgt cgatatctgc accagggagg
    22381 ccgccagggc aggggttccc tgcacggcgc gtttcatcga cgcgctctct cttgacatcc
    22441 tgatgcaccc cgagcgctac gacgtgattg tgacgaccaa catcttcggc gacatcctct
    22501 ccgataccgc agcctacctg gttggcgggc tcggtatgct cccgagcggc aatatagggg
    22561 agcggcatgc gctctttgaa cccgtccacg ggagcgcccc cgatatcgca ggacagggta
    22621 tagcaaaccc cattgcagcg atcaggagcg cagcgatgct ccttgcccat acaggcgacc
    22681 ctggatcagc ggcggtcatc gaggaggcca ttgaccgggt gctcaatgca ggcatccgga
    22741 cgcgcgacct gggcggcaac gccggtaccc gggagttcgg ggcggcggtg ctacgcgagg
    22801 ttctgcggag gaaggcctaa acgctcaagg gtcgagagac tacggtatgg tgatggttgg
    22861 atgccacatc tccatcgccg ggtcgatcga ccgtgcggtt gaccgtgccc gcgatgcggg
    22921 ttgtgacacg tttcagatct tctcccggaa cccaagaggc tggagggcga gggacctgga
    22981 cccggacgtg gtcgaggctt tccgggcggc ggtgagatcg tcagggctcg gcccggtctt
    23041 tgaccacatg ccctacctcc cgaacctggc ctcgcccgac gatgccatct atgagaagtc
    23101 agttgctgcg cttgcagatg aactcaggcg atgcgccctt ctcggtatac cctatattgt
    23161 gacacacctc ggacaccacc gcggcgccgg gatcgaggag gggcaggaac gggtcatcgc
    23221 cgcgatcaac cgtgcgtttc acgaaaccgg ggatacaggg gtgacgctcc tcctcgagaa
    23281 taccgctggg gagaagaaca gcgttgggac gaggatcgag gatctcgcgc tgatctacga
    23341 tggcctggat gatagggggg agttcgggat ctgttttgat acctgccacg cgtttggcgc
    23401 aggctacgac ctccggacgg cggatgggat cgatgctgtc gttgcggaga tcgatgagcg
    23461 gatcggcgtt gacagcctcc atctcatcca cctcaacgac agcaaggggg atctggggag
    23521 cgggcttgac cggcacgagc atatcggcct tggatccata ggagaggatg ggtttcggga
    23581 gatcctccgc aacccgcaga tccggcgtct ccccctgatc tgcgagacgc cggtcgacgc
    23641 ccggcgggac gatgccggga atatcgcgaa ggtccgcgaa cttgccgggt attgagcaac
    23701 agtagagagt tcccctatga agagagagag cggttcggcc gatgcggatg cgccaccctc
    23761 ccgctggagg gtgcgcggga ggcggcaggc agacctctgc atcgtctggt tctgcatcct
    23821 gggtgcggtt cttgtggggc tcaccgttct tgcgctcgca aacataacaa taactgaact
    23881 tgcagacccg ctccacctca tcgaggttgc ggcttcgtca ggggtgctcg gagcgatcct
    23941 cctcaccttc tctgcggggg caaacgctgt cctcctcctc atactcttcg gaacaccgct
    24001 tgcatacgtc ctcgcccggg cccccccgtc cagggttaag ggggttgtcg agagtatcgt
    24061 cgacctgccg ctcatcctgc cccacacggt tgccgggctg ctcgtctacc tcctcttcat
    24121 gcgaagagga tggctcggcg cgccgctcgc cgagatcggg cttgcatttg aggatgccta
    24181 ccccggcacg gtcgtcgcga tgctcttcgt cgcctcgccg ttctttgtaa acacgatgcg
    24241 ggaagggttt gagaaggtcc ccctacacct tgagaacgtc gcccgcaccc tcggcgccgg
    24301 gaggttcacc accttccgta cggtcaccct ccctcttagc ctgcggcatc tgtttaacgg
    24361 ggcaatcctt gcctggggaa gggcaatagg ggagtttgcc gccgtggtca tgatcgcata
    24421 ctacccgttc atcatatcga cgctgatcta ctactcgttt accacgtccg gcatccgcac
    24481 aagcaggagc atagcgtttg tggtcatagt ggtcagtctg ggggtattct acctgctgcg
    24541 gaggttgacc ggatacctgg ggaggtacga tgatcgcgtt tgaacgggta tcgctcaggc
    24601 tggggtcgtt ctcactgaag gatgtcagtc tctcgatcaa ccggggcgac tactacttca
    24661 tcatcggccc ctctggcgcg ggaaagacgg tgctcctcga ggcgattgcc ggtctccaca
    24721 ggccggatag cggccgtatc ctcctccggg ggaaggagat caccgatctg cccacggagc
    24781 gaagggggat cggcctcgta taccaggact actcgctctt tccgaacatg accgtcatcg
    24841 ataacgtctc ttacagtctt cgcgtccggg gggttcgcag gcaggaggca cgcgagagag
    24901 tcgccggcct ccttgaaagg ttcggtgtcg aacacctggc ggaccgctac cccggaaccc
    24961 tctcgggcgg tgagcagcag cgggtggcgc tcgcaagagc cgtcgctgtg aaaccggata
    25021 tcctcctgct ggacgagccg ctctcagcgc tcgacccggt cacgcaggag aggttcatcg
    25081 ccgacctccg gcgactccac cgtgatgaag ggctcacgat cgtgcacgtg agccattccc
    25141 gccgggaggc gcacatgctt gcaacccgga tggcggttct catcgacggt cgcctcgtcg
    25201 acgagggcga tgccgatgtt gtgctgaacg ccccggggag ccgggaggtt gcatctttca
    25261 tcggggttga aaacatcctg gatggagtgg tgacggcaaa ccacggtgga cgtgcaactg
    25321 tggatgcagg tgggttgagg tttgatgcgc agacagacgc tgcagtgggg gaggcggtct
    25381 gcctctgcat ccgtgccgat gacctcatca tcgctgtgga cgacggccgg cggccagacg
    25441 ccccgaacac gatgaccggg acggtcattc agatcgtcga gaacggccct gtggccgaga
    25501 tcctggtgga tgcggggatg gacatgacgg cggtgatgac ccggcggtcg gtgcgggacc
    25561 tcgggattgc gcccggggtt cgggtcagcc tccgtgtaag agaggacgcg atccacgtca
    25621 tccgttctgc gctttcagcg cgagagtgaa ggcgacgagg acggcggtgg caccgttcgc
    25681 gactattatg gcgagatcac ccctcgccgc cccgtagacg aaccagagca ggactccggc
    25741 caggaggaga agaagcatcg cgaacgagag gtcggcggtc gagcggctgc gccatgcctt
    25801 tatggcctgc ggggcaaacg agagcgtcgt aagcgctccg gcaaaaagac caagaattgt
    25861 aactgtatcc atgtgcggat cgatcatctt cccgcctgag gtgatgaaac ttcagccttc
    25921 aggacgtagg ccatgatcat acctataatg aacgcaaacg taagcccgat caccagggtg
    25981 aggacggcta tggccccggt gacggcatac gactcggtct ttatgctgtg ttttccgagt
    26041 tctatcgcca cgaagacaag gagcgcgcca aagaccccga gagggatcag ggtcagcacc
    26101 tccggcgggg cgaatgcgac ggcaaagatg aggataaaaa cgccagcgat gatgtttgcc
    26161 ccgccggttc gcgcaccgaa gcggtacatg gcggcaagcc ccccggcgcc gtggcacatc
    26221 gggaaccctc caagcggcgt cgagatgagg ttcatggcac cgatcgtacg ggaaaggcgg
    26281 tcggggtcca cacccctctt cgggaagagg tcgtgtgtga ggagagatgt ggcgaggatg
    26341 gcatttgtca gggtgagcgg gatctgtggt atggcaagtt cccaggtgcc ggctatgaag
    26401 tctgagggaa gcgggatgat gagggagggg aggggtatca accggagcgg cggcatcccc
    26461 tgtgtgacga tacccacaca aagtccgatc agtataacaa tcagagcgga gatgtccggg
    26521 atccgggtgc gctgtgaagc gacaaagaat gccaggatga tcccgatgga gagagccgca
    26581 aacagggcgt cgttgaggat gtagtcaagc gacgtccgga gaaggaggag cgcgagaccc
    26641 gcctggacgc cccggatgac acttctcggg atccgatccg caagccaggt catgccgccg
    26701 agaagcccga gcagcaggaa gagcgcgccg acgacgatgc ccgatgccac gatctctcca
    26761 ccggaaagcc ctccggcgat gacgatggca ccgatggcct tcattggctc gatcgggatc
    26821 gggaggcggt agtagaaccc ggctatgatg taccaggcgg ctatgaagag gaagaaatgg
    26881 cttacgttca cgtccgggca gactatggcg acaccaagga ggatgggaaa gatcgtcccg
    26941 aaatcaccga ctgcgccggc aacctcctcc agcgtgaacc tgatacccct ctcttccgcc
    27001 ccggtgctct ccgccatggt gctccttctt ctatctggtc agatgggtct ttgaagttat
    27061 ggattatccc ggccgcccat acg
//
LOCUS       DAOSZX010000010        26997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1471_length_26997_cov_20.531772, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000010 DAOSZX010000000
VERSION     DAOSZX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 26997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26997
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_26997_cov_20.531772"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK196_01800"
     CDS             complement(<1..245)
                     /locus_tag="PK196_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40191.1"
                     /translation="MPLVKIEIRMGKPVEYRRSMIESVRRAFIEALQTPEAALWIRVC
                     ERKEENFPLPPGRSDDALLIEVSLMPGRTAEKKEALFR"
     gene            complement(342..1505)
                     /locus_tag="PK196_01805"
     CDS             complement(342..1505)
                     /locus_tag="PK196_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HON40192.1"
                     /translation="MMPEEQHNPCENPLWPCAMTGAVACLAGFEDVAVVVHGSSGCYF
                     YPASLIGAPIYGTFLLENEVIFGTESRLREVIAEIGDRYSRIAVVNTCVPAIIGEDMS
                     DPGHGGRVLFIDSPGFLGYLETGYRAALEAISPEVDPAAPGVNIGGISLFDPFCRGNA
                     IEARRLLNLAGVPVQTTFCLDRYAATRRAAPFTVETNPDLAPGVGTTCGSLLGLDALG
                     ETFERLGAAIDGVDARPVAEEAAWADARIRKGCDKYLRRFDPPVVAIAGGAAYAEYAA
                     DLLDRYLGADIAFIAARNDPGPGKTGITHTTDLALIREMIMDAEPDLILGSSYEQALA
                     PDAAFVGLTPPLRDRVTLSSPAVAGVEGALRLMEAVLNACIDRHRRRGSSAVD"
     gene            complement(1532..2653)
                     /locus_tag="PK196_01810"
     CDS             complement(1532..2653)
                     /locus_tag="PK196_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HON40193.1"
                     /translation="MQYRVAAIGGDGIGPEIIAAGREVLDAAGERYGFDIDWTEFDMG
                     AERYLASGELLTEEDIQELSKFPAIYFGAIGDERVRPGILEKGILLAIRFHFDQYINL
                     RPIRLLEGVQTPLANKRPEDIDFVVVRENTEDFYVGIGSRFAGGREQKTLEVLREMYS
                     IRFGLDVETDAEELAYQIGVITREGAGRVIRYAFDLAMQRRKKVTSVDKANVLSDVYG
                     LWRDVFSEVAAGYPGVSTEFNFVDAVTMWFLKNPEWFDVVVTPNMFGDIITDLGAMIQ
                     GGLGLAPGGNINPSGTSMFEPIHGSAPKYRGQDVANPFATVWAGSMLLDHIGEHEAAA
                     AVLRAIERSILEGFVTRDMGGAKKTSEVGRHLAALVRTV"
     gene            complement(2638..3129)
                     /locus_tag="PK196_01815"
     CDS             complement(2638..3129)
                     /locus_tag="PK196_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HON40194.1"
                     /translation="MRVWKFGDDIDTDAIIPGRFLTIYDPAELAKHAFEGTRDEFAAG
                     VRDGDIVVAGRNFGCGSSREHAPLALLGAGVKIVVAKSFARIFYRNAINTGLLPLVCP
                     ETDAIQDGEAISVDIGAGCIDLKGRRLPVEPVPDFMKRIVEAGGLVAYARSLDRVETC
                     NTA"
     gene            complement(3135..4388)
                     /locus_tag="PK196_01820"
     CDS             complement(3135..4388)
                     /locus_tag="PK196_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HON40195.1"
                     /translation="MSATIVEKIFSQHCKRPIRAGRVVMVPVDAAMIHDITGPLAIRV
                     FGEMGGERVFDPERIIMLFDHQVPADSIPAAENHVFMRRFAEEQGIRNYDLREGVCHQ
                     VVMEKGRAAPGEIVVGTDSHTCTYGAAGAFATGIGSTDMGFVLKFGALYFRVPESILL
                     EIDGAFGRRVGAKDLILSLAGDIGADGATYMALEFTGRAIRDMDMAGRMTCANMAIEM
                     GAKAGIVPPDATTWGYVTERRAIEPFDLASDPDASYRERRHYDVTDLAPQVAVPHNVD
                     NVVDVTEVAGTRIDQVFIGSCTNGRYEDLEEAAEVLGSADRFAESVRVIVIPASRTEY
                     LKALRAGLIERFVEAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSTAA
                     SVYLSSPATAAASALYGEITDPREV"
     gene            4537..7158
                     /gene="mutS"
                     /locus_tag="PK196_01825"
     CDS             4537..7158
                     /gene="mutS"
                     /locus_tag="PK196_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HON40196.1"
                     /translation="MTDGPTPAMRQYYAVKARYPDAIIFFRMGDFYETFGDDAGVVAR
                     ELDITLTARGRDRNGERMPLAGVPHHAADGYIARLVSKGYKVVICDQVEDPKTAKGIV
                     KREVTRVITPGTLIDSSMLGSAGAHYLMAVAPDHGGSFGLAFLDVSTGEFFVSSGSGE
                     RDYADIISEVVRYRPTEAIVPESLEGDLPARLEALGVTVSRCRDDAFDPRAAYEHLCS
                     QFGTTTLDGYGCAQMTGAIAAAGAALLYAQETQQSPLPHISGLSTRVPSENMLLDAIT
                     LRNLEITAPIRGGNDANTLLSTLDLTETPMGSRTMRSFLIAPLLRKDAIEARLDAVEW
                     FFDRTIERQALRAALGDVADIERIAGRIAYGNAGPRDLVTLRDSLEAIPGIKALFDAD
                     APPLIREALDMMADHASAADLIARAIVDDPPALARSGGMIREGFNTKLDGLRHLATSG
                     KDWIAEFQQQERERTGIKSLRVGYNRVFGYYIEVTKPNLHLVPPEYERRQTIANGERY
                     TTPDLREKEAMIATAEDRLSALEAEVYADLIRALAAEVPGLQATARAVGLLDVYSALA
                     EVAARYGYTRPRIEESGRILIRDGRHPVVERNLQTPFVPNDTELDSGGTQIMIITGAN
                     MSGKSTYMRAVALCCIMAQMGSFVPARHAAIGIVDRVFTRVGAFDDLASGQSTFMVEM
                     LELANILNNATPQSLVILDEIGRGTSTLDGSAIARAVVEFLHGKAVVGPRTLFATHFH
                     DLIDLEGSLARVKNFHFAVKDTGTDVVFLRKIIPGATDRSYGVHVAQLAGVPKKVTDR
                     ARQLMKEASARQLPDGPRAPLYTQMLLIDPGEGVVEENPAIKELRALNPNEMTPIEAL
                     NTLCRLQRLANGEDEER"
     gene            7155..8903
                     /gene="mutL"
                     /locus_tag="PK196_01830"
     CDS             7155..8903
                     /gene="mutL"
                     /locus_tag="PK196_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HON40197.1"
                     /translation="MTEIHVLDPETVNQIAAGEVVERPASVAKELLENAIDAGATVIL
                     LEIASDAAGITKIQVTDNGEGMTPGEAVLAFQPHATSKIRDITDLSRIRTLGFRGEAL
                     ASIAAVSEVTMVTRPRGGEALSGTRLVVRGGEIVETAEVGAPEGTTVTVERLFYNTPA
                     RRKFLKSRNTELAHVYAVVESLALAHGEIAFRVVHNGKERMATQRSAGLLATIAGLYG
                     ADLARSLLPVEGKLPFLTIRGYVSRPQENRGSPSQISLSINGRSILSRKIAAAVREGY
                     GNLLPKDRYPVAFMDLAIDTGLVDVNVHPTKREVRLSREREILEAVTAAVRKALADHD
                     LSRDAPEEPVQQQIMADDTPPVARETEAVYAAAHRGGPALADKRLRRTEREGEKNLLP
                     AMEPIGQVAATYIAAEGVDGTLYLVDQHAAHERILYDQVVEQRDAESAAQELLVPVIL
                     PLSPKEAAALREAIPLLAGEGFVVEEFGRDTFAVRAVPAALGAVEDAAGTVRETIADL
                     LSGESRAHTDRREAVTCIIACRGAIKAGTRLTPEQQRLLISQLARTKTPWTCPHGRPT
                     VVAFDRKKLEKIFGRR"
     gene            complement(8915..9787)
                     /locus_tag="PK196_01835"
     CDS             complement(8915..9787)
                     /locus_tag="PK196_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HON40198.1"
                     /translation="MGRNTHLLEKTLAVLLVLSVAANIFLLGLILSPAGAGLSPAVPE
                     AEDGVGTPSAALAPASATGGTATMEVPVVIQRIEVRQGGPFPYQEVTTGGGMVNITVE
                     VAPGKGRVLVQTTPLMGTVFQDAANHAVAAAARHSGVDLSGTDVIFSIRSAEEVSEID
                     GPSAGAAMAALLVSVLEGFQINESVTVTGTIDENGNIGAIGGVLEKAEAAYRSGKTIL
                     LLPRENNQVVGYREEVRLLGNLKVVRQLPVAMDAQTCIQETLGIQVRYVETLDDLLTY
                     LRADEGSRTVSGAV"
     assembly_gap    9829..9928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9929..10365)
                     /locus_tag="PK196_01840"
     CDS             complement(<9929..10365)
                     /locus_tag="PK196_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON40199.1"
                     /translation="MSKGDQVYTPAEDSFLLLRAALREVRPGDRVLEVGTGSGYIAAG
                     LVDRAAMVVATDINPHAAASARARGVEVVRTDMAAGISGPFDLILFNPPYLPTAPEER
                     IDDWLEYALDGGPTGRAVIERFIADAGRVLAPSGRILLLISSLT"
     gene            complement(10368..11141)
                     /gene="rsmA"
                     /locus_tag="PK196_01845"
     CDS             complement(10368..11141)
                     /gene="rsmA"
                     /locus_tag="PK196_01845"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HON40200.1"
                     /translation="MSAPRDQHFLIDRRAVEKIAGFADVSGRRVLEIGPGEGILTRAL
                     LERGAEVIAVEIDPALVEELEVTFADEIERGRLDIIQGDARKVDLPPFDIVVANLPYS
                     VSAKITFRLLSIGFETAILMYQKEFVQRMIAPPGTSNVGRLSVMVQTYASVKPLLELS
                     PSAFRPKPQVRSWVVRVMPHEPPYPIVDREVYADVVRVLFSNRRKTVRKGLQGGRAAF
                     PPGTIEGAILDLPAELLQRRPQDLSLEEFAMIANRLTGC"
     gene            complement(11138..11704)
                     /locus_tag="PK196_01850"
     CDS             complement(11138..11704)
                     /locus_tag="PK196_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HON40201.1"
                     /translation="MRAEKKEVNAVVLDVLPMGYMSADQRPGYRREPVVQAVGTDQFK
                     LLELVPKPGADIRIHDRVYIGDEERDKIERVKRRIGYEDLTATAKLELPFVVEQIVRE
                     NEQRFVDFFNKSVPLTPKFHMLHLLPGIGKKLLWEILEERGKKPFESFEDISRRIRSL
                     SHPDRMIVTRILREIEDPNEKYHVFTSR"
     gene            complement(11724..>11906)
                     /locus_tag="PK196_01855"
     CDS             complement(11724..>11906)
                     /locus_tag="PK196_01855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HON40202.1"
                     /translation="ARALVAELMKLEKMKEDIAYRIANLMPRTRDELRAIYAKERFNL
                     TTEELDEILELVMTHF"
     assembly_gap    11907..12006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12007..12266)
                     /locus_tag="PK196_01860"
     CDS             complement(<12007..12266)
                     /locus_tag="PK196_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HON40203.1"
                     /translation="MAHHNGPRKKTRYKFKKDLRKRGIPPVTSVIQDFEIGQRVHVVV
                     EPSVQKGMPHRRFHGKTGTVIGRRGRAWVLEVRDGNTIKQVIA"
     gene            complement(12269..13507)
                     /locus_tag="PK196_01865"
     CDS             complement(12269..13507)
                     /locus_tag="PK196_01865"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HON40204.1"
                     /translation="MGITEEVEAILEYGEICDHCLGRFYGKRSFGLTNAERGRALRIA
                     CEVSANRPHREPDPASCWVCGGELSRIGDWAEKVAGALSGIEFETLLIGTRVPPLVAE
                     GEEMVWSDLALHDPEPLKAEMNREVGKAVSGLTGKNADLKNPDVVAILDLGEGTVEVQ
                     VNPVFFAGRYLKYERGIPQTHWDCRACRGAGCERCNYTGKQYLDSVEELIGRPAVEVF
                     EAKNAILHGAGREDIDARMLGSGRPFVMEIEAPRKRLVDLAALEEKINRDAAGRVAVR
                     LDGWADRKMVQSIKSGKGHKRYRILVEIDGSVTPDEFRAALDQLKGATIRQRTPTRVS
                     HRRADKVRERRVLDIECTGGVDGRYWVEVAGEAGLYIKELVSGDSGRTRPSLAQILGR
                     TAHVVSLDVVQVETANEYGE"
     gene            complement(13498..14076)
                     /gene="trmY"
                     /locus_tag="PK196_01870"
     CDS             complement(13498..14076)
                     /gene="trmY"
                     /locus_tag="PK196_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HON40205.1"
                     /translation="MKRFAIVGHVAETGGNFSLNDLPGSGGRMDILCRCISSSLFLSH
                     DLRRDVECYLVLCGEPDPEKTVLFCGSGVRRLSPDERSSAALIKKALSIPCGDEFRES
                     TPGVYVRRGGLSRLLAEIPFAVLDEAGEDLRRAPELPENYLLSDHQNFTPAEEEAIAG
                     AARYSVGPRSLHADHAITVLLNEIDRRESGWV"
     gene            complement(14073..15404)
                     /locus_tag="PK196_01875"
     CDS             complement(14073..15404)
                     /locus_tag="PK196_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HON40206.1"
                     /translation="MLDTLGNSLKDAVKKLSGKTVIDRAAVDELVRDLQRALLSADVN
                     VKLVMQLSQSIKKRALEEEPPKGMGVRAHVLRIVYQELVRLMGRPGEVSLEPQTILMA
                     GLQGSGKTTTAAKLARYFQRKGLRTGVICADTFRPGAYDQLKTLCDRISVPCFGKPGE
                     PDALAIVREGLSAFRRQYEVIIIDTQGRHALEENLIEEIIQINEIAHPDHRWLVIDAA
                     LGQQASEQARRFHEAIGIDGVIVTKMDGTAKGGGALSAVAETRSGIVFIGSGETIEDL
                     ERFDPDGFISRLLGMGDLKALVERAEEAVSTDEIDVNAMLKGKFTLRDMYKQLEALNR
                     MGPLKQIMSMLPLGGMQIPDDAYDITSTKMQRYRVIMDSMTPSELDDPSIIGSSRVQR
                     IARGAGVAPEDVRELIKYYKIMQRALKGMRGMDSGRFNMQRMMKKFGGPGK"
     gene            complement(15404..16465)
                     /gene="ftsY"
                     /locus_tag="PK196_01880"
     CDS             complement(15404..16465)
                     /gene="ftsY"
                     /locus_tag="PK196_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HON40207.1"
                     /translation="MFDSLKKKLQNIRTKFASNIEEAAGVGVEPPQPPGKAEAAAPLA
                     RPESPAVLEEREKPTLINRVKVFVREREILIQEKDIEEPLSELEMVLLENDVALPVTD
                     EIISRMRAELVGRHRRIGDSVDDLVVSTLRSALCGVLGEGFDLLRYVEEHERPVKILF
                     TGVNGTGKTTTVAKVGHYLQKNGFSVVIGAGDTFRAGAIEQIVTHADRLGIRVVRHQP
                     GADPSAVLFDAVQYAKAHNIDVVLADTAGRFHNRANLMNQLEKIRRVMKPDLIVYVDE
                     AVAGNDAVIRADEFNRTVGTDAVVLTKADMDPRGGAAISIAHTVGKPILFLGTGQGYD
                     DIMPFEPLAVVNELLGGEV"
     gene            complement(16468..16914)
                     /gene="pfdA"
                     /locus_tag="PK196_01885"
     CDS             complement(16468..16914)
                     /gene="pfdA"
                     /locus_tag="PK196_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HON40208.1"
                     /translation="MEQVDPREIQTLQVYLNEYAQQIEILTEQLSMIEQQRIEAAAAI
                     ETLRAIQENEGATVLLPVGGGALLRVKVLDTGHAIVNIGADVSVERSTEDAVNYLEGR
                     ITELEALGKKVAGSLEQLQARATEISRRLEAAYGAARQAQAGQGGS"
     gene            complement(16916..17107)
                     /gene="rpl18a"
                     /locus_tag="PK196_01890"
     CDS             complement(16916..17107)
                     /gene="rpl18a"
                     /locus_tag="PK196_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HON40209.1"
                     /translation="MENQYFEIIGACRIDNDWKPFRKVVTAANENLAKERVFTDIGSK
                     HHLKRNYITIESVNVVAGE"
     gene            complement(17114..17776)
                     /locus_tag="PK196_01895"
     CDS             complement(17114..17776)
                     /locus_tag="PK196_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HON40210.1"
                     /translation="MTGTIDLAGDPNIGVFARVFEDIAIVHPAAPAAFTAALERELDV
                     EIVKTPVQESSIIGSLVAGNSRGLVVSGLATDEELAVLSDYRDIFLLEGSMNAAGNVI
                     LANDYVAAVHPGMPVDLAEEIGLFLSVPVIRLSLGSIKTVGMAGFATNRGILVHPRAN
                     DTEIASIERVVDLPVGLGSVNMGSGLVGTGLLANSKGYLAGSATTGIELGRIEEVLGF
                     LE"
     gene            complement(17778..18041)
                     /locus_tag="PK196_01900"
     CDS             complement(17778..18041)
                     /locus_tag="PK196_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HON40211.1"
                     /translation="MVEPLKEHVYVIPLRDVKRAPRWKRGKIAMKDIRAFLARHMKSE
                     DVKLDQSINEKIWERGGEKTPGKIRVRAMKFEDGQVQAELAEE"
     gene            complement(18047..18199)
                     /locus_tag="PK196_01905"
     CDS             complement(18047..18199)
                     /locus_tag="PK196_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HON40212.1"
                     /translation="MSKLTKGRKIRLAKACEQNRRVPAWVMIRTNRNVTSHPKRRNWR
                     RSSLKV"
     gene            complement(18222..18812)
                     /locus_tag="PK196_01910"
     CDS             complement(18222..18812)
                     /locus_tag="PK196_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HON40213.1"
                     /translation="MEAGLLFSGGKDSALAATMLARDYGTELNTCVFDPDREVPQVRA
                     AAAFLGLPFRKRVLGRDLLDEAVDLLLACGYPNDAIDTVHRAAVEMLAGDYAVVADGT
                     RRDDRVPGLDRSEIQRLEMTTGCSYVRPLLGYGKPEVERLAGKLFLVVYGETGSIGNG
                     DYEQEIRSAIRARGIDPAPFFPSHHLQSLVVGRRKT"
     gene            complement(18815..19153)
                     /locus_tag="PK196_01915"
     CDS             complement(18815..19153)
                     /locus_tag="PK196_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HON40214.1"
                     /translation="MVDDELAELRRRRLEQMQRQAMEQQAMEEELARQQQVDSQIRTV
                     LMEILEPEARERLNTIKLTRPDFARGVEQQLVMLAQSGRIRQRISDEQLKTILVQLTP
                     SKKDFRITRK"
     gene            complement(19172..19621)
                     /locus_tag="PK196_01920"
     CDS             complement(19172..19621)
                     /locus_tag="PK196_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HON40215.1"
                     /translation="MTTVYDIPADMLIKRVAEELKKNPQIQPPDWAAFAKTGVHKQMP
                     PENDDWWYLRAAAILRRIYTDGPVGTQRMRSAYGGKRDRGSAPYQFRRGSGSIVRKIF
                     QQLEAAGYVARSSAGRTITPAGRSFLDNVAHSLKEEAAEVAPQLANY"
     gene            complement(19690..19932)
                     /locus_tag="PK196_01925"
     CDS             complement(19690..19932)
                     /locus_tag="PK196_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HON40216.1"
                     /translation="MSRESFQSLRPTVWIGKRGVTSVMIDEIQRQLKDRKVVKVRWLR
                     NTEVDPVEVADVVGAVLAEVRGRTLVLKERRVDRPG"
     gene            complement(19929..20246)
                     /locus_tag="PK196_01930"
     CDS             complement(19929..20246)
                     /locus_tag="PK196_01930"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HON40217.1"
                     /translation="MADTTQKSGSRRLARERIVILFERAAEFYPENPEWSNRCVELAR
                     RIGMRHRVRIEPPLKRRFCRRCNSYLVPGSNARVRIHRGRVVVTCLKCGHRSRYPIGR
                     SKL"
     gene            complement(20312..20941)
                     /gene="purN"
                     /locus_tag="PK196_01935"
     CDS             complement(20312..20941)
                     /gene="purN"
                     /locus_tag="PK196_01935"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HON40218.1"
                     /translation="MEQTIPLRKKRIGFLASGRGSNFQAVIDAIAAGEIPGIGAGLIT
                     DNPRAYAIERARNGGIPVRVIDYAEFPSKAAYEEELLAAMRDFQADLFVLAGYMRILG
                     QEIVREFSGRMMNIHPALLPAFPGLHAQRQAIEYGVKVAGCTVHLVDEGTDTGPIVIQ
                     RCVPVLQDDDETALADRILTEEHRALPLAVKLFCEDRLIVEGRRVRIRP"
     gene            complement(20948..22243)
                     /locus_tag="PK196_01940"
     CDS             complement(20948..22243)
                     /locus_tag="PK196_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HON40219.1"
                     /translation="MVVIDLLAVETVLFIICLLLSGFFSSSEVALISITRAKVRALLN
                     QGRKGAEALDELKRSTDSILITILIGNNVVNVAAASLATAIAIGLYGDLGIGIATGVT
                     VILMLIFGEIGPKMYASRHTERLALAVARPILFLSRLISPLLWVSERIQRHLSSSTSM
                     TEPVVTEEELKEWIDVGEEEGTIEEEERDMLYSVLRFGDTTVREVMTPRVDVVMIEDT
                     ATIENALSIFNETGFSRLPVYHGHIDNVVGLLNVKDVFSAVFRQQAGATIRDLMYEPY
                     FVPESKKIDDLLKELQVKKQHMAVVLDEYGSFAGIVTIEDMLEELVGEIMDEFDEEEP
                     EIQQIEGGVYLVDARAWVERINEDLKLNLPLMDTYESIGGLVIERLGHIPRRGEVVRI
                     EESNVTLVVMQMRGRRIVKVKLIIEPRVVREGGGEEPKG"
     gene            complement(22338..23138)
                     /locus_tag="PK196_01945"
     CDS             complement(22338..23138)
                     /locus_tag="PK196_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON40220.1"
                     /translation="MFFHEYPCDQIFDYIAGSGFDSLEFWVETPHFWVRGCPEDELSD
                     CIASHPELSPITVHAPSLDMNPCSINPRVAAISVEYAIEAIRMAERIGAALVTVHPGR
                     RTAKREPSAPDYTRFEAYVAGLREAAEETGVKVAIENMEPRINTLLATAEDTAEVLER
                     EPWLWLTFDMGHAMMNSPTEVGRFLDLCIERMANVHVSALGVNGRPHHPIHDDPAALR
                     VLSDLAERGYGGWLTLEVDDMIFPGALSAEEKIVLLSRELAALREVFS"
     gene            23205..24449
                     /gene="xseA"
                     /locus_tag="PK196_01950"
     CDS             23205..24449
                     /gene="xseA"
                     /locus_tag="PK196_01950"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HON40221.1"
                     /translation="MPGSHATSPERFGSPILRVSEVSGLICDLLDDPRLHQIWVRGEV
                     TNYRDHTSGHRYFSLSEQGAGRTAVINCVMWRSVASTLSFRPKDGMDVLAWGSVEVYE
                     PHGRYQFIVREMIPAGAGERHLMVERWKRELEAEGLFDPGRKRPLPVFPRRIGVVTSS
                     SGAALKDILSVISRRYPVEVVVSPTAVQGEGAHTEIAEAIRRVDGLVDVIIVGRGGGS
                     FEDLFPFNHPDVVRAVAACRTPVISAVGHEVDTALCDFAADLRAPTPSAAAERAVPER
                     QEVLRELGGYEERMLSLVQHRLAAAQNDVDDLRERMHPRRLARRVHERMQRLAEMEER
                     LERAAFGRLQRERSDLAELRTRLDAQNPLSVLERGYSIIEADGRVVRSARDLRPGARV
                     ALRMKDGRANAVVEEITYDRDF"
     gene            24433..24618
                     /gene="xseB"
                     /locus_tag="PK196_01955"
     CDS             24433..24618
                     /gene="xseB"
                     /locus_tag="PK196_01955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HON40222.1"
                     /translation="MTETFEEMLEELRDIIRKLEDGDTSLEESIAIYERGALLVKRCE
                     DLLAAAEMKLTELGRDR"
     gene            complement(24652..25089)
                     /locus_tag="PK196_01960"
     CDS             complement(24652..25089)
                     /locus_tag="PK196_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HON40223.1"
                     /translation="MKARDTVRARGDPLVRATHPTTFEVTKDMRLTGAGDCIIGVAAD
                     KGAADLDPDLRALLRDDRAVLTTRLAAGGKTVIVRSRGSSAFSLDHPTDLVWRRSDFI
                     SGRTVGIRSDRVAATLPREFVAALQRGEELVVELEAEIPGEGD"
     gene            25122..25748
                     /locus_tag="PK196_01965"
     CDS             25122..25748
                     /locus_tag="PK196_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HON40224.1"
                     /translation="MISIVCPVCKEEYEHQILRETTDLVVQCCECGHVHRVEKPAEPA
                     TITIKAIVSHETESKVCSLDMFEEETVSLGDRLAAECGDEAFGVEVTGIEVGAKRVQR
                     ATARDVTCLWTRKIDQVVVRASIHTGRTTIPLYQTMDGEDELVVGETRSFAGRRIRIS
                     HIKLRDGPLMRKEGWKTMARRVKRVYGYLDGRSRFNDTAAPGGKGERR"
     gene            complement(25835..26113)
                     /locus_tag="PK196_01970"
     CDS             complement(25835..26113)
                     /locus_tag="PK196_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HON40225.1"
                     /translation="MAVRVICFYQEGCMGCEEQTPILREVEKELGIEIEEIDAVRNPE
                     YIRTYKLRVTPTILVLEDDVVRERMEGLVHREELEAAIRRHMPEPAPE"
     gene            complement(26159..26860)
                     /locus_tag="PK196_01975"
     CDS             complement(26159..26860)
                     /locus_tag="PK196_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40226.1"
                     /translation="MKKTQQKNSKDAGAGRMKWAYVAIAVLIAVAMVGTYLAPILAKK
                     PAAQVGDTAVIDYTIFTEDGRPVITTDQTLLESEYRKGNSGLLLTQRLEMTAGAQVSG
                     ENVAVLPVVYPPITGFSGFGLLGFETNAISAGLIGMRQGETKTISFSYGGNDLEMNLS
                     QEDADGIGLNFTQAAVGDMITLGLTTSPEIPLGGETNSTTALRFGQVIDKTDDSLVII
                     YRYGSASVTLNGITG"
BASE COUNT         5227 a         8617 c         7641 g         5312 t
ORIGIN      
        1 cggaagaggg cctccttctt ctcggcggtg cgccccggca taaggctgac ctcgatcagg
       61 agagcgtcgt ccgaccggcc tggaggcagc gggaagttct cctctttgcg ctcgcagacc
      121 ctgatccaga gggcggcctc cggggtttgc agcgcctcga tgaacgccct gcggacgctc
      181 tcgatcatcg acctccggta ctcgaccggt tttcccatac ggatctcaat ctttacaagc
      241 ggcattcaga ccacatccgg caggccgcgg ggagacggag acggctcccc cgcttacctg
      301 tatgagcgtt ccgatagccg ggtcttaacg ctttccaggc atcagtccac cgcagaggaa
      361 cccctgcgcc ggtgccggtc gatacaggcg ttgagcaccg cctccatcaa ccggagcgca
      421 ccctcaacac ccgcaaccgc gggtgaagag agggtgaccc tgtccctgag cgggggcgtc
      481 aacccgacga acgcggcatc cggggcgagc gcctgctcgt aggacgagcc aaggatcagg
      541 tcgggctctg cgtccatgat catctcccgg atcagggcaa ggtcggtcgt atgggtgatc
      601 ccggtcttcc cgggacccgg gtcgttcctg gcggcgataa aggctatatc cgcaccgaga
      661 taccggtcga gaaggtcggc agcgtactca gcgtaggcgg cgccgcccgc tatcgccacc
      721 acaggaggat caaaccggcg caggtatttg tcgcagcctt tcctgatcct ggcatccgcc
      781 cacgccgcct cctccgcgac cgggcgggca tcgaccccat cgatcgcggc tccaagccgc
      841 tcgaaggtct cgcccagggc atcaagaccg aggagcgaac cacaggttgt gccgacaccg
      901 ggcgcaagat ccgggtttgt ctcgacggtg aacggcgccg cccgccgggt tgcggcgtag
      961 cggtcgaggc agaacgtggt ctgcacaggc acaccggcga gattgagcag ccgccgcgcc
     1021 tctatggcgt ttccccggca gaaagggtcg aaaagagaga tcccgcctat gttgacgccc
     1081 ggcgcggcgg gatcgacctc cggtgagatc gcctcaagcg ccgccctgta accggtctcg
     1141 agatagccga ggaacccggg actgtcgatg aacagaaccc ggccgccatg gccgggatcg
     1201 ctcatatcct cgccgatgat cgccggcaca cacgtgttga ccacggctat ccggctgtag
     1261 cggtcgccga tctccgctat aacctcccgc agccttgact ccgtgccgaa tataacctcg
     1321 ttctcaagaa gaaacgtccc gtatattgga gcgccgatga gcgaggcggg gtagaagtag
     1381 cagccgcttg aaccgtggac gacaacggca acatcctcaa acccggccag gcaggccacc
     1441 gcaccggtca tcgcgcaggg ccagagcggg ttctcgcagg gattgtgctg ctcttctggc
     1501 atcatggcaa aaggcaggga ttacaaaccc ttcaaaccgt ccggacgagc gcggcaagat
     1561 ggcgccccac ctcgctcgtc ttcttcgccc cgcccatatc cctggtgacg aacccctcga
     1621 ggatactccg ctcgattgcc cggagaaccg ccgccgcggc ctcgtgctcg ccgatgtgat
     1681 ccaggagcat cgagcccgcc cagacggtgg caaacgggtt tgcaacgtcc tggccgcggt
     1741 acttcggtgc cgaaccgtgg atcggttcaa acatcgaggt gccggagggg ttgatgttcc
     1801 cgcccggcgc aagaccgagt ccgccctgta tcatggcgcc gagatcggtg attatatcgc
     1861 caaacatgtt cggggtgacg acaacatcga accactcggg gttcttcagg aaccacatcg
     1921 tgacagcgtc gacaaagttg aactccgtcg agacgcccgg ataacctgcg gcaacctccg
     1981 aaaagacgtc ccgccagaga ccgtagacgt cggagaggac gtttgccttg tcgaccgagg
     2041 tgaccttctt cctccgctgc atggcaaggt cgaaggcgta gcggatgacc cgccctgccc
     2101 cctcgcgggt gatgacaccg atctggtatg caagttcctc ggcatcggtc tcgacgtcca
     2161 gaccgaacct gatgctgtac atctcccgca gaacctccag cgtcttctgc tcgcgccccc
     2221 cggcaaacct ggatccgatc ccgacataga agtcctccgt gttctccctg accaccacga
     2281 aatcgatatc ctccggacgc ttattcgcaa gcggcgtctg gacaccctca agcagccgta
     2341 taggccggag gttgatgtac tggtcgaaat ggaaccggat agcaagcagt atacccttct
     2401 ccagtatacc gggtctcacc cgctcatcac cgatcgcccc gaagtagatc gcgggaaact
     2461 tcgagagttc ctggatatcc tcctccgtca ggagttcgcc tgatgcaagg tatcgctcgg
     2521 cgcccatatc gaactctgtc cagtcgatat caaacccgta acgctctccc gcggcgtcca
     2581 ggacctccct gcccgctgct atgatctcgg ggcctatccc atccccgcct attgcggcta
     2641 cgcggtattg catgtctcca cccggtccag gctcctcgca taggccacga gaccgccggc
     2701 ctcgacgatc ctcttcatga aatcaggcac aggctcgacc ggaagccttc ttcccttcag
     2761 atctatgcag cccgctccta tatccaccga gatggcctcg ccgtcctgga tggcgtcggt
     2821 ctccggacag accagcggca ggagcccggt gttgatcgcg ttccggtaaa agatcctggc
     2881 aaacgacttc gctaccacga tcttcacacc ggcgccgagc agagcaagcg gcgcatgctc
     2941 acgcgacgac ccgcacccga agttgcggcc ggcgaccacg atatccccgt cacgcacccc
     3001 tgctgcaaac tcatcccgcg tcccctcgaa cgcatgtttt gcaagttccg ccggatcgta
     3061 gatcgtcagg aacctgccgg ggatgatagc atccgtatcg atatcatctc caaacttcca
     3121 tacccgcatg attctcacac ctccctcggg tcggtgatct cgccgtagag tgcgcttgcc
     3181 gctgccgtcg ccggcgagga gaggtagacc gatgccgcgg tgctcccctg ccggccccgg
     3241 aagttccggt tggatgtcga gagcgagacc tcgccaggtg caagcagccc gaacgctccg
     3301 cccatacagg ggccgcagca tggcgcctca acgagcgccc cggcctcaac aaaccgttcg
     3361 atgagtccgg cccggagagc cttcagatac tccgttcggg aggcggggat gactatcacc
     3421 cggacgctct ccgcgaagcg gtcggccgac ccgagcacct ccgccgcctc ctccaggtcc
     3481 tcgtaacggc cgttggtgca ggaacctatg aagacctgat cgatcctcgt tccggcaacc
     3541 tccgttacgt ccacgacatt atcgacgttg tgcgggacgg caacctgcgg ggcgagatcg
     3601 gtgacatcgt agtgcctccg ttccctgtat gaggcatcgg ggtcgcttgc aagatcaaac
     3661 ggctcgattg cgcgccgttc ggtgacgtag ccccatgtgg ttgcatcggg cgggactata
     3721 ccggccttcg cccccatctc tatcgccatg ttcgcacacg tcatccggcc cgccatatcc
     3781 atatcgcgta tcgcccggcc cgtgaactcg agcgccatgt atgtcgcgcc atcggcaccg
     3841 atatcgcctg cgagggagag gatcaggtct tttgccccca ccctccggcc aaacgctcca
     3901 tctatctcaa gcaggatgct ctccggcacc cggaagtaga gcgccccgaa cttcaggacg
     3961 aaacccatat cagtcgaacc gatcccggtc gcaaacgctc cagcggcgcc gtaggtgcag
     4021 gtgtgggagt cggtcccgac gacaatctcg cctggcgccg ccctcccttt ctccatcacg
     4081 acctgatggc agacgccttc gcgcaggtcg tagttgcgga tcccctgctc ttctgcaaac
     4141 cgccgcatga agacgtggtt ctcggcggcg ggtatcgagt cggcgggcac ctggtggtca
     4201 aagagcatga tgatccgttc cggatcgaat acgcgctccc cacccatctc gccaaagacc
     4261 ctgattgcaa gcgggccggt gatgtcgtgg atcatcgccg catccaccgg caccataaca
     4321 accctgcccg cccggatggg tcttttgcag tgctgcgaaa atatcttctc tacgatagtc
     4381 gccgacatac agtatccttc tcttctgtag gtgctcggga cgtatgtagt ttgccggaac
     4441 aagggaggct ggtgcagttg agtccgggct cgtttcggcc gattttgcac ggcagtagtc
     4501 ttatgtttct ccgcgcggta cttcttaaca gaagcaatga ccgacggacc taccccggca
     4561 atgcggcagt actatgctgt gaaggcacgc tacccggacg ctatcatctt ctttcggatg
     4621 ggagatttct atgagacctt cggggatgat gccggtgtgg tggcccgcga actcgatatc
     4681 accctcaccg cccggggcag ggaccgaaac ggtgagcgga tgcctcttgc aggcgtcccc
     4741 caccacgccg ccgacggcta catcgcacgc ctggtgagca agggctacaa ggtggtcata
     4801 tgcgaccagg tggaggaccc gaagaccgct aaggggatcg tgaaacggga ggtcacccgt
     4861 gtgatcaccc cggggacgct gatcgactcc tcgatgcttg gttcggccgg cgcccactac
     4921 ctgatggcgg tcgcccctga ccatgggggc tccttcggcc tcgccttcct ggacgtctcc
     4981 acaggcgagt tctttgtatc atctggcagc ggggaacgcg attacgccga tatcatatcg
     5041 gaggttgtgc gttaccgccc gacggaggcg atcgtacccg agagccttga aggtgacctc
     5101 cccgccaggc ttgaggcact cggggtgacg gtgagccgtt gccgcgacga tgccttcgac
     5161 ccccgggccg catacgagca tctctgcagc cagttcggga cgacaaccct cgacggctac
     5221 ggctgtgcgc agatgacggg agccatcgcc gcggcaggtg cagcgctcct ttacgcccag
     5281 gagacacagc agtcgcccct ccctcacatc tccgggttat cgacccgggt gccatctgag
     5341 aatatgctgc tcgatgcgat caccctccgg aacctggaga tcacagcccc catccgtgga
     5401 gggaacgatg caaacaccct cctctcaacc ctcgatctca cggagacacc gatgggaagc
     5461 cggacgatga gatcgtttct gatcgcgccg ctgctccgga aggatgccat cgaggcgcgg
     5521 ctagatgcgg tggaatggtt ctttgaccgc accattgagc ggcaggcgct tcgcgcggct
     5581 ctcggcgatg ttgccgatat agagcggatt gccggaagga tcgcctatgg aaacgccggc
     5641 cctcgcgacc ttgtaacgct gagggactcg ctcgaagcga tccctggcat aaaagcgctc
     5701 tttgacgccg atgctccgcc gctcatccgc gaggccctgg atatgatggc cgaccacgcc
     5761 tcggctgcag acctcatcgc ccgggctata gtcgacgacc ccccggcgct cgccagatcg
     5821 ggcggcatga tccgcgaggg gttcaacacg aaactcgacg gcctccggca cctggcaacg
     5881 tccgggaagg actggatcgc cgagtttcag cagcaggaac gggagaggac gggtatcaag
     5941 tcgctcaggg tcggctacaa ccgtgtcttt ggctactaca tcgaggtgac aaaacccaac
     6001 ctgcaccttg ttcctccgga gtatgaacgc cggcagacga tcgcaaacgg cgaacgctac
     6061 acgacccccg acctccggga gaaggaggcg atgatcgcga ccgctgagga ccggctctcg
     6121 gcacttgaag cagaagtcta cgccgacctc ataagggcac tcgcagcgga ggttcccggg
     6181 ctgcaggcga ccgccagggc tgtagggctg ctcgacgttt attcggcgct cgccgaggtt
     6241 gccgcacgct acggctacac caggccgagg atcgaggaga gcggccggat cctcatccgc
     6301 gacggccgcc accccgtggt ggagcggaac ctccagaccc cgtttgtccc caacgacacc
     6361 gaacttgaca gcggcggcac ccagatcatg atcatcaccg gggcgaacat gtcgggcaag
     6421 tccacctaca tgcgggcggt ggcgctctgc tgcatcatgg cccagatggg gagtttcgtc
     6481 ccggcgcggc atgcagccat cgggatcgtc gacagggtct tcacaagggt gggggcgttt
     6541 gacgacctgg catccgggca gtccacgttc atggtcgaga tgctggaact cgcaaacatc
     6601 ctcaacaacg caacaccgca gagtctcgtc atcctggacg agatcggccg gggtacgagc
     6661 acgctggacg gttctgccat cgccagggcg gttgtggagt tcctgcacgg aaaagcggtt
     6721 gtgggcccgc ggacgctctt tgcaacccat ttccacgatc tgatcgacct tgagggctcg
     6781 cttgcgcggg tgaagaactt ccatttcgcg gtgaaggata ccggaaccga tgttgtcttc
     6841 ctccgtaaga tcatccctgg cgcaacggac aggagttacg gtgtccacgt cgcccaactc
     6901 gcaggtgtcc cgaagaaggt gaccgaccgg gcaagacaac tcatgaaaga ggcatcggca
     6961 cggcagctcc ccgacggccc ccgggcgccc ctctacaccc agatgctcct gatcgatccg
     7021 ggcgagggcg ttgtggagga gaaccccgcg atcaaagagc tcagggcgtt gaacccgaac
     7081 gagatgacgc cgatagaggc tttgaacacc ctatgccgtc tccagaggct tgcaaacggc
     7141 gaggatgaag agcgatgaca gagatccacg tcctcgaccc cgagaccgtg aaccagatcg
     7201 ccgccggaga ggtcgtggaa cggccggcat cggtggcaaa ggaactcctg gagaacgcta
     7261 tcgacgccgg ggcaacggtt atcctgcttg agatcgcatc ggatgctgcc gggataacta
     7321 agatccaggt gaccgacaac ggagagggga tgacgccggg agaggccgtc cttgcgttcc
     7381 agcctcacgc gacgagcaag atccgggata tcaccgacct ctccaggatc aggacactcg
     7441 ggtttcgagg ggaggcgctc gcgagcatcg ccgcggtctc ggaggtgacc atggtcaccc
     7501 ggccgcgtgg aggcgaagcg ctcagcggca cccgcctggt ggtcaggggc ggcgagatcg
     7561 tggagacggc cgaggtaggg gcgccggagg ggacaacggt caccgtcgaa cggctcttct
     7621 acaacacacc cgcccgcagg aagtttctaa agagcaggaa caccgaactt gcccacgtct
     7681 acgcggtcgt tgagagcctg gcgctggcac acggcgagat cgccttcagg gtggtgcaca
     7741 acgggaaaga gcggatggca acccagcgat ccgccggact gcttgcaacc atcgcggggc
     7801 tgtatggtgc cgacctggcc cgatcgctcc tccccgtcga ggggaagctt ccgttcctta
     7861 ccatccgcgg ctacgtatcc cggccccagg agaacagggg gagcccctca cagatctctc
     7921 tcagcatcaa cggacgaagc atactctccc gcaagatcgc cgccgccgtc agggagggtt
     7981 acggaaacct cctccctaaa gacagatacc cggtagcgtt catggacctt gcgatcgata
     8041 ccgggcttgt tgacgtcaac gtccacccga ccaagcgaga ggtccggcta tccagggagc
     8101 gcgagatcct ggaggcggtc accgcagcgg tgaggaaagc gctcgcagac cacgatctct
     8161 cacgggatgc gccagaagag cccgtgcagc agcagatcat ggcggatgat acgccaccgg
     8221 tggcgcggga gactgaagcg gtctacgcgg ccgctcaccg cggcggaccc gccctggccg
     8281 ataaacgcct ccgccggaca gagagggagg gggagaagaa ccttctgccg gcgatggagc
     8341 cgatagggca ggtggccgca acctacatcg cggcggaggg ggtcgacggc accctctacc
     8401 tcgtcgacca gcatgccgcc catgaacgga tcctctacga ccaggtcgtg gagcagcgtg
     8461 atgccgaatc cgccgcacag gaactgctcg tcccggtcat cctccccctc tctccgaaag
     8521 aggccgccgc gctccgggag gcgatcccgc tcctggcggg ggaggggttt gtggtggagg
     8581 agttcggccg ggacacattc gccgtccggg cagtccccgc cgccctcggt gcggtggagg
     8641 acgccgccgg cacggtcaga gagaccatcg ccgatctcct cagcggcgag tcccgcgctc
     8701 acaccgaccg gcgggaggcg gtcacctgca taatcgcctg caggggcgca atcaaggccg
     8761 gcacccgtct cacgccagag cagcagcgac tcctgatctc gcagctcgcc cggacgaaga
     8821 ccccatggac gtgtccgcac gggagaccaa cggttgtggc gttcgataga aagaaactcg
     8881 aaaagatctt cgggcggaga tgagtccgca cccttcagac tgcgccagag accgttcttg
     8941 aaccttcgtc tgcacggagg tatgtgagca ggtcatcgag ggtctccacg tacctcacct
     9001 ggatccccag ggtctcctgg atgcaggtct gtgcgtccat ggcaaccggc agttggcgca
     9061 cgaccttgag gttgcctagt agcctgacct cctcacggta accaaccacc tggttgttct
     9121 cccgggggag gaggagaatc gtctttccgc tgcggtaagc ggcctcagcc ttctccagaa
     9181 caccgccgat cgcgcctata ttcccgttct catcgattgt ccccgtcacc gtcacgctct
     9241 cgttgatctg gaagccttca agcaccgaga ccagcagcgc ggccatggcc gctcctgcgc
     9301 tgggcccatc gatctcggag acctcttccg cgctcctgat gctgaagatg acatcggtcc
     9361 ctgataggtc cacgccggag tgcctggcgg cagcagcgac cgcgtggttt gcagcgtcct
     9421 ggaagaccgt ccccatcagc ggcgttgtct gaacgagaac acgccctttc cctggcgcaa
     9481 cctcaaccgt gatgtttacc atcccgcccc ccgttgtaac ctcctggtag gggaaggggc
     9541 cgccctgcct gacctcaatg cgctggatga ccaccggcac ctccatcgtg gcggtcccac
     9601 ccgtcgctga ggctggcgcg agcgcggccg agggggtgcc gaccccatcc tccgcttccg
     9661 ggacggccgg ggataggccg gctcctgcgg gggagaggat gagacccagt agaaagatat
     9721 ttgcagcaac cgataacacg agcagaaccg ccagggtctt ttccagcagg tgtgtattgc
     9781 gccccatgaa tgtactctat tctttcggag aagatgtaac ttgcgcggnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cagggaggag ataagcagca gtatcctgcc
     9961 tgaaggcgca agcacccgac cggcatcagc gatgaatcgc tcaataaccg ctctcccggt
    10021 cggcccgccg tccagggcgt actccagcca gtcgtcgatc cgctcctccg gggcggtcgg
    10081 gaggtatgga gggttgaaga ggatgaggtc aaacggcccg gaaatcccgg cagccatatc
    10141 cgtccgtaca acctccaccc ccctggcgcg ggcggatgca gcggcgtgag ggttgatatc
    10201 ggttgcaacc accatcgccg ccctgtccac gagaccggcc gcgatataac cgctccccgt
    10261 ccccacctcc agcacccggt cgcccggcct gacctcacgg agcgccgccc gaagaagtag
    10321 aaacgagtcc tcggccggtg tgtagacctg gtcgcccttg ctcatgctca gcacccggtg
    10381 agcctgtttg caatcattgc aaactcctca agcgagagat cctgcggccg gcgctgcagg
    10441 agttcggcgg gaagatccag gatcgctccc tcgatcgtgc cgggaggaaa cgccgccctc
    10501 ccgccctgca gccctttccg cacagtcttt cgccggttgg agaagagcac ccgcacaacg
    10561 tcagcgtaga cctcccgatc cacgatcggg tacggcggtt cgtgcggcat gacccggaca
    10621 acccaggagc ggacctgcgg tttcgggcgg aaggcagagg gtgagagttc gagcaggggt
    10681 tttacggaag cgtaggtctg caccatcacc gagagccggc cgacgttaga ggttcccggc
    10741 ggcgcgatca tccgctggac aaactccttc tggtacatca ggatcgccgt ctcaaacccg
    10801 atcgagagca gccggaacgt aatctttgca gaaacagagt acggcaggtt tgcgacgacg
    10861 atatcgaacg gcgggagatc aaccttcctc gcatccccct ggattatatc cagacgcccc
    10921 ctctcgatct cgtcggcaaa cgtgacctcg agctcctcca caagggccgg atctatctct
    10981 accgcgataa cctctgcacc ccgttcaagg agcgctcgag tcaggatccc ctccccgggg
    11041 cctatctcga gcacccttcg gccggagaca tcggcaaagc cggcaatctt ctcgaccgcc
    11101 ctgcggtcga tgagaaagtg ctgatccctt ggagcgctca tctcgatgta aagacgtgat
    11161 acttctcgtt cggatcctcg atctcgcgca ggatccgggt gacgatcatc ctgtccgggt
    11221 gggaaagcga cctgatccgc ctggagatat cctcaaaact ctcaaacggt ttcttcccac
    11281 gctcttccag gatctcccac agcagtttct tcccgatgcc ggggagaagg tgcagcatgt
    11341 gaaacttcgg tgtgagcgga acggacttat tgaagaaatc aacgaaccgc tgctcgttct
    11401 cgcggacgat ctgctcgacc acgaaaggca gttcgagttt tgctgtcgcc gtcagatcct
    11461 cgtagccgat ccgccgcttg acacgctcga tcttatcacg ctcctcatcc ccgatgtaga
    11521 cgcggtcgtg gatccggata tccgcgcctg gtttcggaac aagttcgagc agtttgaact
    11581 ggtcggtgcc gaccgcctgg acgaccggtt cgcgcctgta gcccgggcgc tggtcagcac
    11641 tcatataacc catgggaagg acatcgagaa ctaccgcgtt cacctctttc ttttcggctc
    11701 tcatatgctc cacccccgag gggttagaaa tgggtcatta ccagttcgag gatctcgtcc
    11761 agctcttccg tcgtcagatt gaaacgttct tttgcgtaga tggcccgcag ttcatccctg
    11821 gttcgcggca tcaggttcgc gatgcggtag gcgatatcct ctttcatctt ctcgagtttc
    11881 atcaactcgg caacaagggc gcgggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnngcaa taacctgttt tatcgtattg ccgtccctga cctcgagcac ccatgcacgt
    12061 ccacgccgcc cgataacagt accggtcttc ccgtggaacc gccggtgggg catccccttc
    12121 tggacgctcg gttcgaccac tacgtgcacc ctctgcccga tctcaaagtc ctggatcaca
    12181 gaggttactg gggggatgcc acgttttcta aggtccttct tgaacttata ccgcgtcttc
    12241 ttgcgtggtc cattgtgatg tgccatgatt actcaccata ctcgtttgct gtttcgacct
    12301 gcaccacatc gaggctgact acatgtgcgg tgcgccccag gatctgggca aggctgggcc
    12361 tggttctgcc gctgtcaccc gataccagtt ctttgatgta gagacccgct tcaccggcga
    12421 cttctaccca gtatctgccg tcaacgccac cagtacactc gatatcgagg acccgccgtt
    12481 cccgaacttt atctgcccgc cggtgcgaca cccttgtcgg ggtgcgctgc cgtatcgttg
    12541 caccttttaa ctggtcgagg gctgccctga actcatctgg tgttacagaa ccgtcgatct
    12601 caaccaggat cctgtatctc ttatgccctt tgcctgattt aatgctttgc accatcttcc
    12661 ggtccgccca gccgtcaagg cggacagcca cacgcccggc agcatccctg tttatcttct
    12721 cctcaagcgc cgcaagatcc accaaccgct tccggggagc ctcgatctcc atcacaaacg
    12781 gccggcctga gccaagcatg cgggcatcga tatcctcccg tcctgccccg tgcaggatcg
    12841 cattcttcgc ctcaaagacc tcgaccgccg gccggcctat cagttcctca acggagtcga
    12901 gatactgttt tccggtgtag ttgcaccgct cgcaccccgc tccgcggcac gcccggcagt
    12961 cccagtgggt ctgcgggatc ccgcgctcat acttcaggta tcgcccggca aagaagaccg
    13021 ggttgacctg cacctcgaca gtgccctccc cgagatcgag gattgcaaca acgtcagggt
    13081 ttttaaggtc ggcgttcttc ccggtgagcc ccgatacagc ctttccaacc tccctgttca
    13141 tctcggcttt gagcggttcg gggtcgtgaa gggcaaggtc gctccagacc atctcttcac
    13201 cctccgcaac aagcggcggc accctcgtcc ctataaggag agtctcaaac tcaatacccg
    13261 ataaagcccc ggcaaccttt tctgcccagt caccgatcct ggagagttcg ccgccacaga
    13321 cccagcagga ggccggatcc ggctcccggt gcggtctgtt tgccgagacc tcgcaggcga
    13381 tcctgagcgc ccggcccctc tcagcgttgg tcagcccaaa cgacctcttc ccgtagaacc
    13441 gtccgagaca gtgatcacag atctccccgt actcaaggat tgcttccacc tcttcagtta
    13501 tacccaaccc gactccctcc ggtcaatctc gtttaagagg actgtgatgg catgatcggc
    13561 atgcagcgac cgcggtccga ccgagtagcg ggcagccccg gcgatggcct cttcctccgc
    13621 cggcgtaaag ttctggtggt ctgagaggag atagttctcc gggagttctg gagccctcct
    13681 gagatcctcc ccggcctcat cgagcaccgc aaacgggatc tctgcaagca gccgtgaaag
    13741 tccgccgcgg cggacgtaga ccccgggcgt cgactcccgg aactcatccc cgcatgggat
    13801 ggagagagcc ttcttgatga gggcagcgct gctccgttcg tccggcgaga ggcggcgcac
    13861 cccgctcccg caaaagagaa cggtcttctc cggatcaggc tctccgcaga ggacgaggta
    13921 acactccaca tcccgccgca ggtcgtggga gaggaacaac gaggaactga tacagcggca
    13981 gagaatgtcc atcctccctc cgctgccggg gagatcgttc aggctgaagt tgccgcccgt
    14041 ttcggcgacg tggcccacga tggcgaatcg tttcatttac ccggtccgcc gaacttcttc
    14101 atcatgcgct gcatgttaaa cctgccgctg tccatgccgc gcatcccctt caaagcgcgc
    14161 tgcattatct tgtagtattt tatgagctcc cggacatctt caggcgccac cccggcgccg
    14221 cgggcaatcc gctggacgcg cgaactccct atgatcgacg ggtcgtcgag ctctgagggc
    14281 gtcatcgagt ccattatcac ccgatagcgc tgcatcttgg tgctggtgat atcgtaggca
    14341 tcgtccggga tctgcatccc ccccagcgga agcatgctca tgatctgttt gagaggcccc
    14401 atcctgttca acgcctcaag ctgtttgtac atatcccgga gtgtgaactt ccccttgagc
    14461 atcgcgttta catcgatctc atcggtcgaa accgcctcct cagcacgctc tacaagcgct
    14521 ttcaggtcgc ccatcccgag cagccgcgat atgaatccat cgggatcgaa cctctccaga
    14581 tcctcgatcg tctcaccgct cccgataaag acgatcccgc tccgggtctc agcgaccgcc
    14641 gagagagccc cgccgccctt cgcggtgccg tccatcttgg tgactatgac gccgtctatc
    14701 ccgatagcct cgtggaaccg gcgggcctgc tcgcttgcct gctgccctag cgccgcatcg
    14761 atcacgagcc acctgtgatc cgggtgcgcg atctcgttaa tctggatgat ctcctcgatc
    14821 agattctcct ccagggcgtg ccgaccctgg gtatcgatga tgatcacctc atactgcctg
    14881 cgaaacgcag agagaccttc acggactatc gcaagagcgt caggctcccc cggcttgcca
    14941 aagcatggaa cgctgatcct atcacagaga gtctttaact ggtcgtaggc gccaggccga
    15001 aacgtatcgg cgcagatcac ccctgtccgc aaccctttgc gctggaagta tcgggcaagt
    15061 ttcgctgccg ttgttgtctt accgctcccc tgcagccctg ccataaggat cgtctgaggc
    15121 tcaagcgaga cctcgccggg cctgcccatc aatctgacaa gttcctggta gacgatccgg
    15181 agaacgtgcg cccggacacc catccctttc gggggctcct cctcgagagc gcgtttcttg
    15241 atcgactggg agagttgcat caccagtttg acgttgacat cggcggagag gagagcccgc
    15301 tggagatcgc gcaccagttc atcgacagcc gccctgtcga tcacggtctt tccggagagt
    15361 ttcttgactg cgtccttcaa tgagttgccc agggtgtcga gcatcacacc tcacctccca
    15421 gcagttcgtt gaccaccgcc aggggctcaa acggcatgat gtcatcatac ccctgccctg
    15481 tcccgaggaa gaggatcggt tttccaacgg tgtgtgcgat cgatatcgcc gcgcccccac
    15541 gggggtccat gtccgccttg gtcaggacga ccgcatcggt gccgactgtc ctgttgaact
    15601 catcggcccg gatgaccgca tcgttcccgg ccaccgcctc atcgacgtag acgatgagat
    15661 caggcttcat cacccgccgg atcttctcca gctggttcat aaggtttgcc cggttgtgga
    15721 aacgcccggc cgtgtcggcc aggaccacat cgatgttgtg cgcctttgca tactgaacag
    15781 cgtcaaatag aacagcagag gggtcggcac cgggctggtg gcggacaacc ctgatgccaa
    15841 gacggtcagc atgggtaacg atctgctcga tcgcgccggc ccggaaggtg tccccggcac
    15901 cgatcacaac cgagaacccg ttcttctgca ggtagtgacc tacctttgca acggttgtcg
    15961 tctttcctgt cccgttcacg ccggtgaaga ggatcttcac cgggcgttcg tgctcctcga
    16021 cgtagcgcag gaggtcaaac ccctccccca gcacaccgca gagggccgaa cgcagcgtcg
    16081 acacaaccag gtcgtccaca gagtcgccga tcctccggtg ccggccaacc agctccgccc
    16141 gcatccggga gatgatctca tcggtgaccg ggagggcaac atcgttctcc agaagaacca
    16201 tctcgagttc agagagcggt tcctcgatat ccttctcctg gataaggatc tcccgctccc
    16261 tgacgaagac cttcaccctg tttatgagcg tgggcttctc ccgctcctcc agcactgctg
    16321 gagattctgg cctggcgaga ggggccgcag cctccgcctt ccctgggggt tgcgggggtt
    16381 caacccccac cccggcggcc tcctcgatat tcgaggcaaa tttagtccgg atattctgaa
    16441 gtttcttctt taaagagtca aacattatca acttccgccc tggccagcct gagcctgccg
    16501 ggccgccccg tacgccgcct caaggcgtct ggatatctcc gtggcccgtg cctgcaactg
    16561 ctcaagcgaa cctgcgacct tctttcccag cgcctcaagt tcagttatcc ggccttcaag
    16621 gtagttcacc gcatcctctg tagacctctc aacagagacg tccgccccga tgttcacgat
    16681 tgcatgcccc gtatcaagaa ctttcacccg cagaagagcc ccgccgccga ccgggagaag
    16741 gacggtggcg ccttcgttct cctggatcgc acggagggtc tcgatggcgg cggcagcctc
    16801 aatccgctgc tgttcgatca tcgagagctg ctctgtcagg atctcgatct gctgggcgta
    16861 ctcgtttaag tacacctgca gggtctgtat ctcgcgaggg tctacctgct ccacatcact
    16921 caccagctac tacgttaacc gattcgattg tgatataatt tctcttcagg tggtgtttgc
    16981 tcccgatatc agtgaaaacc ctctctttag ccaggttctc gttcgctgcg gtgacaacct
    17041 tgcgaaacgg cttccagtcg ttatcgatcc tgcacgcacc tataatctca aaatactggt
    17101 tctccatgac ttatcactcc aaaaacccaa gaacttcctc tattctaccc agctcaatcc
    17161 ctgtcgtggc ggatccagcg agatagccct tgctgtttgc gagcaaccct gtgcccacaa
    17221 ggccgcttcc catgtttacc gacccgagac cgaccgggag atcgaccaca cgctcgatgc
    17281 tcgcgatctc tgtatcgttg gccctcggat ggacaaggat acccctattc gtcgcgaacc
    17341 cggccatgcc aaccgtcttg atgcttccaa gtgagagccg gatcaccggc acggatagga
    17401 acaacccgat ctcttcggca aggtcgaccg gcatccctgg atgaacagca gcaacgtaat
    17461 cgttcgcgag gattacgttg cccgccgcat tcatcgagcc ctcgagcagg aagatatccc
    17521 ggtagtcgct caggacggcg agttcctcgt cggtggcaag gccactgacg accagtcccc
    17581 ggctgtttcc agcaacaagc gaaccgatga tgctgctctc ctggactggg gtcttcacga
    17641 tctcaacatc gagctcacgc tcgagcgccg ctgtgaacgc cgccggcgca gcgggatgga
    17701 ctatcgctat atcctcaaag acgcgggcga agacgccgat atttggatcc ccggcaagat
    17761 cgatcgttcc ggtcatctca ctcctcggcg agctcggcct ggacctgccc gtcctcaaac
    17821 ttcatcgcgc ggacgcggat ctttccgggc gtcttctcgc ccccccgctc ccagatcttc
    17881 tcgttgatgc tctgatcaag tttgacgtcc tcactcttca tgtgccgcgc caggaacgcc
    17941 ctgatatcct tcatagcgat tttacctcgc ttccaccgcg gtgcacgctt cacgtcgcgg
    18001 agggggatga catatacatg ctccttcaat ggttctacca tagttcctac accttcaggc
    18061 tgctccgtct ccagttccgc cgcttcgggt gcgacgtaac attacggttg gttctgatca
    18121 taacccatgc aggcacacgg cgattctgct cgcatgcctt tgccagccgg atcttccggc
    18181 cctttgtcaa cttactcatg attctcactc tcgataatta atcacgtctt tcttctgccg
    18241 acaaccagag actggaggtg atgggatggg aagaacggtg ccgggtcgat gccgcgggca
    18301 cggattgcac tgcggatctc ctgctcgtag tcgccgttgc cgatgctgcc agtctccccg
    18361 tagactacca gaaaaagctt tcctgcgaga cgctccacct caggcttacc gtagccaagg
    18421 agcggtctga catacgagca gccggtggtc atctccaggc gctggatctc agagcggtcg
    18481 agccctggaa cacggtcgtc ccggcgggtg ccatctgcga ccacagcgta gtctccggcc
    18541 agcatctcta ccgccgcccg gtggacggta tcgatcgcat cattcgggta accgcaggcc
    18601 agcagaaggt ctacagcttc atcgagcaga tcgcgcccga gcaccctttt acggaaaggc
    18661 agaccgagaa aagccgcggc ggcacgcacc tgcgggacct cacggtcagg atcgaagaca
    18721 caggtattca gttctgtgcc gtagtcgcgc gcaagcatgg tcgccgccag cgcgctgtct
    18781 ttccctcccg agaagagcaa accagcttcc atccctattt ccgcgttatc ctgaaatctt
    18841 tctttgacgg tgtcaactgg accagtatgg tttttaactg ttcgtcgctg atccgttgcc
    18901 ggatccggcc gctctgggcc agcataacca gttgctgttc aacacctctt gcaaaatccg
    18961 gccgtgtcaa cttgatggtg tttagcctct ccctcgcttc aggctcaagg atctccatca
    19021 gaacagttcg gatctgcgaa tccacctgct gttgccgtgc cagttcctcc tccatagcct
    19081 gctgctccat cgcctgccgc tgcatctgct ccagccgccg gcgacggagt tccgcgagtt
    19141 cgtcatcaac catcagccac gcctccttcg atcagtagtt tgcaagttgc ggtgcaacct
    19201 cagcagcctc ttccttcagg ctatgagcga cgttgtcaag gaacgatctg cccgccgggg
    19261 tgatcgtacg tcctgcactt gacctggcga cgtaaccggc cgcctcgagc tgctgaaaga
    19321 tctttcgcac gatcgaaccg cttccgcgcc tgaactggta tggggccgac ccacggtcgc
    19381 gtttaccgcc gtaggcagag cgcatcctct gcgtcccgac cggtccgtcg gtatagatgc
    19441 gacgaagaat cgccgccgcc cgcagatacc accagtcatc attctccggc ggcatctgct
    19501 tgtgtacccc ggtctttgca aatgcggccc agtcgggggg ctggatctgc ggatttttct
    19561 tgagttcttc tgccacccgc ttgataagca tatcggcggg gatatcatat acagttgtca
    19621 tggatgatcc tcactctcgc taacagtctt tacatattcg tccagtaatg ttttatatat
    19681 ttcgaggatt caacccgggc gatccacccg ccgctccttg agaacaagtg tccgaccgcg
    19741 aacctcggca agaaccgcac ctacaacatc cgccacctcc acaggatcga cctcggtgtt
    19801 gcggagccat cgcaccttga caaccttcct gtctttgagc tgtctctgga tctcatcgat
    19861 catgacgctc gtgacacccc gtttcccgat ccagacggtg ggtctgaggc tctggaacga
    19921 ctccctgctc ataactttga cctcccgatc gggtatcgcg accgatgtcc gcatttaagg
    19981 catgtgacaa ccacccgacc ccggtggatc ctgacccggg cgtttgaccc gggtaccagg
    20041 tatgagttgc acctacggca aaatctgcgc ttcagcggcg gttcgatccg cacccggtga
    20101 cgcataccga tcctgcgggc cagttccacg caccggttgc tccactcggg gttctccggg
    20161 tagaactccg cggcccgctc aaagagaatg acgatccttt cccgggcaag cctgcgggag
    20221 cctgattttt gtgtcgtgtc tgccatggtg cgatcagttc cagcggtgga tcttgaatta
    20281 ggggtgagag gctatataaa ttgatgccgg cctacggccg gatccggaca cgccgcccct
    20341 caacaatcaa gcggtcttcg cagaagagtt taactgcaag ggggagggcc ctgtgctctt
    20401 cagtaaggat ccggtcggcc agtgccgtct cgtcatcgtc ctggagaaca gggacgcatc
    20461 gctggatgac gattgggccg gtatccgttc cttcatccac caggtggact gtgcagcccg
    20521 caaccttcac cccgtactcg atcgcctgcc tctgggcgtg cagtccaggg aacgcaggaa
    20581 gcagggcggg gtggatgttc atcatcctgc ccgagaactc gcggacgatc tcttgcccca
    20641 gtattcgcat gtagcctgcg aggacaaaga gatccgcctg gaaatcccgc atcgcggcca
    20701 ggagttcttc ctcgtatgcg gcctttgaag ggaactctgc atagtctatg acccgcaccg
    20761 ggataccacc atttctcgct cgttcgatcg cataggcacg ggggttgtca gtgatgagcc
    20821 cggcgccaat tcccgggatc tcgccggcag cgatggcgtc gatcaccgcc tggaagtttg
    20881 accccctgcc ggaggccaga aagccgatgc gcttttttcg aagaggtatc gtttgctcca
    20941 tgatccatca tcctttgggc tcttcgccgc cgccttccct gaccactcga ggttcgatga
    21001 tcaacttcac cttgactatc cgccggccac gcatctgcat caccacaagg gtgacgttgc
    21061 tctcctcgat cctgaccacc tcgccgcggc gcgggatgtg ccccagcctt tctatgacca
    21121 gccctcctat gctttcgtag gtatccatca gcggaaggtt gagtttcaga tcctcgttta
    21181 tgcgctccac ccatgcccgc gcgtcgacca ggtatacacc cccctcgatc tgctggatct
    21241 cgggctcctc ctcatcgaac tcgtccatga tctcaccgac aagctcctcg agcatgtcct
    21301 caattgtcac tatcccggca aacgagccgt actcgtcgag gacaacggcc atgtgctgtt
    21361 tcttcacctg gagttctttt aagagatcat cgatcttctt gctctccggg acgaagtaag
    21421 gctcgtacat caggtctctg atcgtcgctc ccgcctgctg ccgaaagacc gcagagaaga
    21481 cgtcctttac atttaagagc ccgactacgt tgtcgatatg cccgtggtag acggggagcc
    21541 tggaaaaacc ggtctcgttg aatatggaga gggcgttctc gatggtcgcc gtatcctcga
    21601 tcatgactac atccacacgg ggcgtcatca cctcgcgaac cgtggtatca ccaaaccgta
    21661 gaacggaata gagcatatcc cgctcctcct cctctattgt gccctcctct tcacctacat
    21721 cgatccactc cttgagctcc tcctctgtaa cgaccggttc ggtcatcgag gtgctggatg
    21781 aaagatgccg ctggatgcgc tccgagaccc agaggagagg agagatcagc cgcgagagaa
    21841 acaggatcgg cctcgcgaca gcgagcgcca gcctctcggt gtgcctggaa gcatacatct
    21901 tcggcccgat ctcaccgaag atcagcatca ggatgacggt aacaccggtt gctatcccga
    21961 tacccagatc accatagaga cctatggcga tcgcggtcgc aagcgatgca gcggccacgt
    22021 tgaccacatt attccctatc aggatggtga taagtatgga atctgtcgag cgcttcagct
    22081 cgtcaagcgc ttccgctccc tttcggccct ggtttaagag ggcgcgtacc tttgcccgtg
    22141 ttatcgatat gagggcgact tctgaacttg agaagaagcc cgaaagaagc agacaaataa
    22201 tgaataaaac tgtttctaca gccagaagat ctattactac catttactcc acgccgcata
    22261 gaacggcagc atccggcact cgcatcttgc cgggattaat ggatcaggtc tcattcagat
    22321 ataataaaac cagcgcttca ggagaacacc tctctcaaag ccgccaattc ccgcgaaagg
    22381 agcacaatct tctcttcagc tgaaagtgcc cccggaaaga tcatatcatc cacctcaagg
    22441 gtgagccagc ccccataccc gcgttcggca aggtctgaaa gcacccgcag cgccgccggg
    22501 tcgtcatgga ttgggtggtg cggccggccg ttcactccaa gagcactgac gtgaacgttt
    22561 gccatccgct cgatgcagag atcaagaaac cggccaacct ccgttggaga gttcatcatg
    22621 gcatgcccca tatcaaacgt gagccagagc catggctccc gttcgagaac ctcggcggta
    22681 tcctctgccg ttgcgagcag tgtgtttatt cgcggctcca tattctcaat cgccaccttc
    22741 accccggtct cctccgctgc ctccctgagc ccggcaacgt acgcctcaaa ccgcgtgtag
    22801 tcgggggcgc tcggctctcg tttcgccgtt cgtctcccgg gatggacggt gaccagagcc
    22861 gcgccgatcc tctctgccat ccggatcgcc tcgattgcgt attcgaccga gatcgcggca
    22921 acccgcgggt tgatggagca ggggttcatg tccagcgagg gggcatggac ggtgatgggt
    22981 gagagttcag ggtggctggc gatacaatct gaaagttcgt cttcagggca gccgcgaacc
    23041 cagaagtgcg gtgtctcaac ccagaactca agagaatcaa agcctgaccc cgcgatgtaa
    23101 tcgaagatct ggtcgcaggg gtactcgtga aagaacatgg ttgagacggc gaaacggccc
    23161 ataatactac ttgatataaa ccattggcct aataggttgt gatgatgccg ggcagtcatg
    23221 ccaccagtcc ggaacggttc ggcagcccga tcctccgggt ttcggaggtt tccgggctta
    23281 tctgcgacct ccttgatgat ccacggctgc accagatctg ggtgcgggga gaggtgacca
    23341 actaccgcga ccacacctcc ggccatcgct acttctcact ctccgagcag ggcgccggga
    23401 ggactgcggt gatcaactgc gtcatgtggc gcagcgttgc atcgacgctc tcattccgcc
    23461 ccaaagatgg tatggacgtc cttgcctggg ggtctgtgga ggtctacgaa ccgcacggca
    23521 ggtaccagtt tatcgtccgg gagatgatcc ctgcgggggc tggcgagcgt catctcatgg
    23581 tcgagcgctg gaaacgtgaa ctcgaagccg aagggctatt tgaccccggg cggaaacgcc
    23641 ctcttccggt ctttccccga cggatcggcg tggtaacatc gagcagcggc gcggcgttga
    23701 aggacatact ctccgtcata tcaaggcgct acccggtcga ggtggtcgtc tccccgacgg
    23761 ccgttcaggg ggagggggcg cacacagaga tcgcggaggc gatccggcgg gtcgacggtc
    23821 ttgtggacgt aataatcgtc ggccgcggcg gcgggagctt tgaggacctc ttccccttca
    23881 accacccgga tgttgtgcgg gccgttgccg catgcaggac acccgtgatc agcgccgtcg
    23941 ggcacgaggt ggataccgcc ctctgtgatt ttgctgccga cctccgggcg ccgacaccct
    24001 cggcggcggc agaacgggcg gtgcccgagc ggcaggaggt gctccgtgaa ctcggcggat
    24061 acgaggaacg tatgctctct cttgtgcagc accgcctggc cgccgcacag aacgatgttg
    24121 atgacctgcg ggagcggatg cacccccggc ggctcgcgcg ccgggttcac gaacgtatgc
    24181 agcgtcttgc cgagatggag gagcgccttg agcgggcggc gttcgggcgg ctgcagcgtg
    24241 aacgctcgga tcttgcggaa ctccgcacga ggcttgatgc tcagaacccg ctttcagtgc
    24301 tcgaacgcgg ttactccatc atagaagcgg atggcagggt cgtgcggagt gcacgcgatc
    24361 tccggccggg cgcgcgggtt gcactccgga tgaaggatgg cagggcgaac gctgttgttg
    24421 aggaaataac gtatgacaga gacttttgaa gagatgcttg aggaactccg ggatatcatc
    24481 cgtaaactcg aggacggtga tacaagcctc gaggagagca tcgccatata cgagcgcggg
    24541 gcgctccttg ttaagcggtg cgaggatctc cttgccgcag ccgagatgaa actaacagaa
    24601 ctcgggcgcg accgctgaga gattggaact acggcggttg ctcgccttcg atcagtcgcc
    24661 ctcgccgggt atctccgcct cgagttctac caccagttcc tccccccgct ggagcgccgc
    24721 cacgaactcc ctggggaggg tcgctgcaac ccggtccgag cggatgccaa cagtccggcc
    24781 ggagatgaag tcactccgcc gccagacgag atcggtgggg tgatcgaggc tgaacgctga
    24841 actgcctctt gagcggacta tgaccgtctt gccccctgcc gcaagccggg tcgtcagcac
    24901 cgcccgatcg tcccggagca gcgcccggag gtcggggtca aggtcggcgg cccccttatc
    24961 ggcggccacg ccgatgatgc agtctccggc ccccgtcagt ctcatatcct tcgtcacctc
    25021 gaacgttgtc gggtgggttg cccttaccag cggatctcca cgggcacgca ccgtatccct
    25081 tgccttcatg cttattagga gtgggatacc actgctaatg aatgatcagc atcgtctgcc
    25141 ctgtctgcaa ggaagagtac gagcaccaga ttctccggga gaccacggat ctggttgtgc
    25201 agtgctgcga atgcggccat gtacaccggg tggagaaacc cgccgaaccc gcgaccatca
    25261 ccatcaaggc aattgtgagt catgagaccg aatcgaaggt ctgcagtctc gatatgttcg
    25321 aggaggagac cgtctccctc ggtgaccggc tcgcggcgga gtgcggtgac gaggcattcg
    25381 gggtcgaggt caccggtata gaggttggag caaaacgggt tcagcgggca acggccaggg
    25441 atgtgacctg cctctggacg aggaagatcg accaggtcgt tgtgagggcg tcgatacaca
    25501 ccgggcgaac gaccatcccg ctctaccaga cgatggatgg cgaggatgaa cttgtcgtcg
    25561 gggagacacg ctcttttgcg ggcagacgta tccgtatatc ccatatcaaa ctccgtgacg
    25621 gccccctgat gcgaaaagag ggctggaaga ccatggccag acgggtaaaa cgggtctacg
    25681 gctacctgga cgggcgttcc aggttcaatg acaccgcagc gcccggaggg aaaggcgaaa
    25741 gaaggtaacc cgtggtgagc ggggcggcag tcgaaaccta accgcgaggt ttccgccgcg
    25801 aaccatgacc cccggactat tatgacctct caggtcactc tggtgcaggc tccggcatgt
    25861 gacgccggat cgcggcctcg agttcctcac ggtggactag cccctccatc cgttcccgga
    25921 ccacatcgtc ctcgaggacc aggattgtcg gggttacccg gagtttatat gtcctgatgt
    25981 actccgggtt cctcacggca tcgatctcct ctatctcgat cccgagctcc ttctccacct
    26041 cgcgaagtat aggagtctgc tcctcgcagc ccatgcaccc ttcctggtag aagcatatca
    26101 cccttaccgc catacatgga agtgagagca cagggattat aaaaaactgt gggatcccct
    26161 acccggttat gccgtttagc gtgacactgg cgctgccgta gcggtagata atgaccagcg
    26221 agtcatcggt cttatcgatg acctggccga aacgcagcgc cgtggtcgaa ttggtctcgc
    26281 caccgagcgg gatctccggg gatgtcgtca gaccaagcgt aatcatatca cccacagcgg
    26341 cctgtgtgaa gttgaggccg atcccatctg catcttcctg gctgaggttc atctcaaggt
    26401 cgttgccgcc ataggagaaa ctgatggtct ttgtctcacc ctgccgcatt ccgataagcc
    26461 cggcggatat ggcgttggtc tcaaaaccaa gcagaccgaa acccgagaag ccggtgatcg
    26521 gggggtatac gaccgggaga acggcaacat tctcgcccga gacctgcgca cccgctgtca
    26581 tctccaggcg ctgtgtcagg aggaggccag aattcccttt cctgtattcg ctctcaagga
    26641 gagtctgatc ggtcgtgata accgggcgcc cgtcctcggt aaatatggtg tagtcaatca
    26701 ccgctgtgtc tcccacctgc gcggcaggct tcttcgcgag gatcggagcc aggtaggttc
    26761 ccaccatggc aaccgcaatc aagacggcaa tcgccacata ggcccatttc atccgcccag
    26821 cgcccgcatc ctttgaattc ttctgttgcg tctttttcat caggagtaca gtcaccgtcg
    26881 ggtaagataa tagtgttctg gaggaggcca tcaaaccaat gcctcactgg tttgataaac
    26941 ctctccgtgt ccagtccatg gacagattcc ggggggtgac cgggatctgt atgaaga
//
LOCUS       DAOSZX010000011        23196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1825_length_23196_cov_19.010890, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000011 DAOSZX010000000
VERSION     DAOSZX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 23196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23196
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_23196_cov_19.010890"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..103
                     /locus_tag="PK196_01980"
     tRNA            33..103
                     /locus_tag="PK196_01980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Gly,seq:ccc)
     gene            complement(278..1513)
                     /locus_tag="PK196_01985"
     CDS             complement(278..1513)
                     /locus_tag="PK196_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40227.1"
                     /translation="MTENPQGFWRRKAILAALFIAVLLPAGVASADAASPEAAWNVTF
                     TLENENKFDAVAPSADGGIIAIGSTLSRTSGGEEDVMLVKTDGAGNQVWLKRLPGMAP
                     ASVAAAADGGCIAGVYSVSINETDGGAEYQGTSFLIRLDAAGEEEWREALPGMKVSSV
                     AKTPDGGYLAAGWLWNPAGSENETTGVLVKTDGNGTPIWNRTFPGTAVNTAIVASDGG
                     CLVGGTSSPFTYDVGDGFLIRLDADGNTLWSRNYQVPVIFDLEETADGGFVYSGNYWY
                     GLVDQDGDEAWARKMEGLTGYAVGIQPSGGYLVAGTNAASGEGFVFGTAADGAILWKM
                     TFPGSRVYAAASAPGGGYVLAGIQIISTGNSTAWLAGITEPGEETEEPVPTTTAPGFG
                     AAAAGAALLILIATRCRRG"
     gene            1610..2620
                     /gene="endA"
                     /locus_tag="PK196_01990"
     CDS             1610..2620
                     /gene="endA"
                     /locus_tag="PK196_01990"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HON40228.1"
                     /translation="MLATFDGTWVHLKSEGRTLYDQGGYGRPEKDGLCLSPEEALYLM
                     ERNKIEVKDLDFDALLGLFADRPNFIRRYMVYRDIRERGYVIQPGPHDFRVFRRGHKP
                     GSGKSRYLIRVLSERDLIDFDRLDQDALAAVNMRKQYLLAVVDDEDELTYYEVRLQDL
                     PPLSNPPACEAPVEARLLGTYALAHLPPGNRLEEEWYGKRLDAERLLFRPVESIYLMR
                     RRCLEIHHDGGPMTAETLLDAVAEVDIEIREKERVFSDLRDKGYIPRTGYKFGHHFRV
                     YSGKKSHSEMLVHAVPAGTSMPMSAVSRSVRLAHSVKKKMLFACVHNTGIRYVEFGRI
                     KL"
     gene            2631..3887
                     /locus_tag="PK196_01995"
     CDS             2631..3887
                     /locus_tag="PK196_01995"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HON40229.1"
                     /translation="MNSEINPWSNNQTVDLDRLFSEFGIEPIGEVARTLPEMPPFIRR
                     GIVVGHRDYNLIADAIRNKTPFHVLTGFMPSGLPHLGHLMVMKEVVWHVQQGGTGYVA
                     IADREAHAVRGISWEKCRDYGKEYLKALYALGFEGTTYYQSRNERLKDLAFEASTRVN
                     FSELNAIYGFGPDTSLSHAMSVVTQVADILFPQVDAGPAPTIVPVGLDQDPHLRLTRD
                     VAYKLRMFTVEDRGEYISVRSKNAPEEAIEAVHRAFPGSKRYAAHVDIPGLSQSQVEE
                     TVRRIEIDHGGFGFYRPSSTYHIFMPGLQGGKMSSSVPASLFGFYEPDSSVKKKVMAA
                     LTGGRMTLEEQRRLGGDPDSCPVYLLNLFHMLDDDIELADLRRRCESGELTCGQCKKE
                     TLERVRAFLADLRDRMDAVEHLAEEV"
     gene            3892..5340
                     /locus_tag="PK196_02000"
     CDS             3892..5340
                     /locus_tag="PK196_02000"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HON40230.1"
                     /translation="MELTQNEKKLLVALGESGSADATSLAEKMNTRPEAVVQYANLAR
                     ERGLVEVEKHVTRRYRPTEEGLAYVGKGLPERQLFESFGEAIPMRDLQKHPLAKIGIG
                     WMRRKGWITIRDGVVHKTGNAPPGPDEVAFARLGETGEIPAGEEGVLDLVKRGLIEEE
                     EAVNYTISLTPEGRALLAAGVDLREEVGTLTREQIISGAWESLPLRRYDVTKLPRRVY
                     PGKTHPYQRLIDEMRRVLFDMGFAEMSGGIVQSSFWNFDALFQPQDHPAREMQDTFFL
                     AERWPLPEGYERVREMHEHGGDTSSTGWGGRWSAEKAQQCVLRTHTTSLSIQHLAEHR
                     EPPVKAFCIGRVYRREAIDPTHLAEFEQLEGIVMDESVNFRHLLGFLKEFYAKMGFDK
                     VRFRPGYFPYTEPSVEPEVYVEGLGWVELGGAGIFREEVTAPFGIEHPVLAWGLGISR
                     VAMLRLGLRDLRHLYRSDVEWIRETPIYGGGR"
     gene            5343..6998
                     /gene="pheT"
                     /locus_tag="PK196_02005"
     CDS             5343..6998
                     /gene="pheT"
                     /locus_tag="PK196_02005"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HON40231.1"
                     /translation="MAIITLPYRYLERLTGVDRETIIERLPMIGADIERVEDDHVDVE
                     FFPDRPDLYSPEGVARAMRGFLGIEAGLPVYSVRPSGITFSVDPGLADIRPYLGSAVI
                     RNVNLDEESIESLMGLQEALHWAVGRGRGKVAIGVHDLDAVRPPFHYIASPRDRSFVP
                     LDFEREMTMEEILVDHPKGRDYAHLVENFPRFPLIVDADDRVLSFPPIINGEVTRVTA
                     ATKNILLDCTGTDRKAVMTAVNIICTALGEAGASIESVNVDGEEMPTLAPAERLVSVE
                     DCSSLLGIPLTPGEMARLLGRMRFGAEPAGDSRVLVRIPCYRADILHDWDIFEDVAIA
                     YGFENFEATLPAVSTIAAEHPVTTIERAVRSVMTGLGYIEMIPFTLTNERVLYTNMQR
                     PPLPGTLRLLHPISEEQTVVRTDLLPLLMEALFANKHRDLPQRLFAVGDVVEDCLTYQ
                     KAAAVSIHPAADFSEAYAAADVLCREVSIGYTVRESADPAFIDGRRGDIVLDGRVVGV
                     FGEIHPAVLRAFDLEHPVAALALDLRAVPGYPAPPGTPSPGGR"
     gene            complement(6940..7746)
                     /gene="hisF"
                     /locus_tag="PK196_02010"
     CDS             complement(6940..7746)
                     /gene="hisF"
                     /locus_tag="PK196_02010"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HON40232.1"
                     /translation="MVLTKRIIPCLDLKDGRVVKGTHFVGLQDAGDPVELARRYNEQG
                     ADEVVFLDITATREDRGTIIDVVQRAADQLFLPLTVGGGIRSIEDMKQILRAGADKVS
                     INSSAVKDPDLITKGAGRFGTQCIVVAIDVRRNYDMAPGRTPITLADGRECWYEVVTH
                     GGSRPTGLDAVKWAMEAEERGAGEILLTSMETDGTREGFDIPITRAVSESVGIPVIAS
                     GGVGSLEHFYKGFTEGKADACLAASVFHYGEFTVRQVKEYLAERGIPVRL"
     gene            8314..9540
                     /locus_tag="PK196_02015"
     CDS             8314..9540
                     /locus_tag="PK196_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HON40233.1"
                     /translation="MESGDLKIAWARQYMPVLSSIRNRFVEEKPFSGMTIGMALHVEA
                     KTAVLVETLAAGGAEVHITGCNPLSTQDDVSAALNTRKGIHSYARRGAGVEDYYAAID
                     RVLDARPAITIDDGMDLIHRLHTERREMLETVIGGCEETTTGIHRLRAMARDGALMFP
                     VIAVNDTPMKRFFDNVHGTGESSLASIMITTNVLIAGKRLVVAGYGYCGRGVAQKARS
                     LGARVIVTEVDPRRALQAHMDGFDVMTMDQAAPLGDLFVTTTGNRSIITERHFPKMKD
                     GAILANAGHFNVEIDLDWLASHADSTVRRDGIDTYLLDGKAVHVLAEGRLVNLATPKG
                     MGHPIEVMDLSFAVQALSARYIAKHGRELKPGVHDVPSEIDEEVARLKLDALGLSIDR
                     LTPEQEAYMNSWTIGT"
     gene            9614..10864
                     /gene="hisD"
                     /locus_tag="PK196_02020"
     CDS             9614..10864
                     /gene="hisD"
                     /locus_tag="PK196_02020"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HON40234.1"
                     /translation="MWQALDIEAWIAGRRSDLDRVKEPVSQIIARVRAEGDAALLDLT
                     KKFDGIDLEEIAVPKEELGAAYDRVDARLVESLVEAEARISAFHELQRGRDLWLQEME
                     PGITLGVRTTPLSRVGAYVPGGRAAYPSTALMCTVPAKAAGVSEICCCTPPPVSPITL
                     VALDIAGVDEVYRIGGAQAIAAMAIGTETVPRVEKIVGPGNVYVTAAKMLLRDEVEID
                     FPAGPSEIAVLADETANPTFIAADILAQAEHDPNAACVLVTTRPGLADAVGAEVDRMV
                     AGSERREIIMKALENSGYVLVSDLDEGVATIDAIAPEHLSIQVSDPLAVLNRVRNAGS
                     IFVGPYAAVACGDYASGTNHVLPTAGYAAIYSGLDLNHFCRRSTVQVISREGLLAIAD
                     VVETIADAEGLHAHAESVRVRRKG"
     gene            10957..13413
                     /locus_tag="PK196_02025"
     CDS             10957..13413
                     /locus_tag="PK196_02025"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HON40235.1"
                     /translation="MPDEKRKAELKVTGMHCASCALNVERALTAREGVHDARVNLATG
                     TASVDYDPAKTTIADIEQAVADAGYGVLKSTATVRIGGMVCASCASVVEASLTGLDGV
                     YDARVNLAAENAYIVYNPELVTIADIRAAIEDAGYTYLGLEEEIPADIEEKMREEDLR
                     EKFRRFTIGFAVSVPLFVIMLLGIPDMLALPVSFNLILLIITLPVFVYVSAPIFKAAA
                     AALRHRALTMDVMYAMGIGVAYGASILGTFEVLLTPEFAFYDTAVMLASFLTLGRYLE
                     ARAKGKTSDAIRSLVGLRPKTATVIRDGREVEVPVEAVVPGDIIIVRPGEKVPVDGVV
                     VGGESSVDESMITGEPIPVEKREGDAVVGGTLNVNGVLRVRAERVGKEMVLAQIIRLV
                     REAQGSKPSVERIADVAVSYFIPAVLAVATAAFLVWYLVLDASLLFSLTVLISVLVVA
                     CPCALGLATPTAVTVGIGRGAELGLLIRNGEALEISEKLTTVVFDKTGTLTRGKPDVT
                     DIVPLGVAEERLLSIAAAVESNSQHPLAAAIVRRAESAGLDLPPSERFTTFGGLGVSA
                     LVEGAEVLIGNPPFLTEHGVTLPPEAAERIAALQDEGKTVVLVAVGGTLAGVIGIADT
                     LKPTAKSAVSELKRMGLAVMMLTGDNERTAAAIAREVGIENVRAGLMPQEKVEEVRAL
                     QQRGEVVAFVGDGINDAPALAQADVGIAIGSGTDVAIESGDIVLIRDDLTDVVAAIQV
                     SRKVISRIRQNLFWAFAYNAALIPIAAGLLYPFFGITFRPELAALAMALSSVTVVSLS
                     LMLKTYIPPAKKLEVDTDGS"
     gene            13403..13552
                     /locus_tag="PK196_02030"
     CDS             13403..13552
                     /locus_tag="PK196_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HON40236.1"
                     /translation="MAVDPVCGMEVDEAAARFKTEYRGRTYYFCAPGCKKLFERDPEA
                     YLKKP"
     gene            complement(13572..14528)
                     /locus_tag="PK196_02035"
     CDS             complement(13572..14528)
                     /locus_tag="PK196_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON40237.1"
                     /translation="MVDKNVFVGEITGGVPVDAPFVVDSAEIREKKDGGKYIHLTISD
                     RSGRGACKVWGTGSQSAEEIEAFCRGIRPGEVYRIAGYAKVYNGTCEVNVNEGIFSLA
                     EPLPLEGIEPSLFVYAPVDTAAVRQGLARMAAMISDTGISSLVGRVLEETPGFFEAPA
                     AKRRHHACTGGLAEHTLETAENAVALAGGVRRAAMDTDVILAGALLHDVGKASCFRRQ
                     GFSFAALPEYTLIGHTAIGAAAVMQHADGVDPARFAHILHIIMSHHGPHGEIQPRTPE
                     AWTVHFADNASAVLRGVCDDTTDLAPGKGRHGGRCGGAVYRF"
     gene            complement(14602..16251)
                     /locus_tag="PK196_02040"
     CDS             complement(14602..16251)
                     /locus_tag="PK196_02040"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HON40238.1"
                     /translation="MQFLEFARICEHLEGISGRLEMIEEVATVLPGLDETDLPIFVRF
                     IMGRIFPDWSTRKLGVGPNLLYDAVAYVAGVKRETVVEAVNRTGDVGLAVEELLAKKE
                     QTSFFIQELELLDVYRGFERMAAAEGQRSQREKLRVAEGLFGNARPIEGRYLARLMLE
                     ELRIGMGEGNVRDAIARAFGVDVHLVEHAQQVINDLGEVALLARNNPEELSRVRIRPF
                     RPVKMMLAQAGAIADQIAEHGTVAVEYKYDGSRFQFHKVGGRCRIYSRKLEEVTESIP
                     DVARRLMESTDHDVILDGEAIAVREGRPMPFQYVIRRFRRKHEIDSMMEQIELVPQVF
                     DILYLDGESLVDRPLSERRRYLEESLHDHVAPQFRTGDPARAEEIYADALNLGHEGVM
                     VKVLDSPYTPGVRGRLWVKVKPGVETLDLAVIGAEWGEGRRAGMFGSFLLAVQDEGGR
                     LLPVGKVATGITDEVLADLYDLFKDRVIARSGKEVTFEPGVVFEVGYSEIQTSPNYES
                     GYALRFPRFVRVREDKSLDEVETLDSLAERYARQKGQTGSA"
     gene            16350..16583
                     /locus_tag="PK196_02045"
     CDS             16350..16583
                     /locus_tag="PK196_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40239.1"
                     /translation="MQQDEELKDMLRDLIWLNALIATELIQITENTSGILRRAPPPES
                     CIAEHAALRATALDIADRYRPGTMLRQHVEKHS"
     gene            16657..17172
                     /locus_tag="PK196_02050"
     CDS             16657..17172
                     /locus_tag="PK196_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HON40240.1"
                     /translation="MKLLIAIAPERFRDEELDVPRRAFLEAGIDVDIASTVTGRCRGM
                     LGGTAAASLTFDDVNPDDYAGIVLVGGSGSPDYLWGNRKLQSLVRAFFEQGKVVAAIC
                     LSPVVLARAGILAGRQATVYPSPDAIREMKKAGANLVSVPVVADRQVVTGNGPEAAAQ
                     FADAVISKLEC"
     gene            complement(17353..18504)
                     /locus_tag="PK196_02055"
     CDS             complement(17353..18504)
                     /locus_tag="PK196_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40241.1"
                     /translation="MRCAECKGKGLCGLERCPVMSRFYARLQVRPSDHYQGTAPSVFV
                     GSYAYPKVSAGPLMIDDTDNPPDWVARGLQIDDIVGLRARTIRGAGESKSVAGSIQEI
                     ALSSRPLDVEAQFVKPVTFDLRFDGTIAPIGLTGAIRHLDVLDNPRVERPVDRVTSDT
                     DLSATEACGLLHSSGTDVYRITQLLTAGLLGGEKKRHIVPTRWAITAVDDTVSKRLKK
                     KIVRYPPLDGIRVFSASLYGNHIVCILVPGDWKFEMIEVWGRQSLWGQGDETIACDGE
                     EGLIKSGYSPLAGAYYSARLAVAEYLDGVQRSARVLVLRHVTGEYWAPLGTWVVREAA
                     RNAMRGPGTPCASLDQAVDLASRLVGFSHWQPYSRLIPELRTQKTLFDF"
     gene            complement(18508..19527)
                     /locus_tag="PK196_02060"
     CDS             complement(18508..19527)
                     /locus_tag="PK196_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HON40242.1"
                     /translation="MNAVMVVHGPAAFDAGDVERLMGLLCPRRVLVAGVMARTAAEES
                     GLPVTCTDERPSRVLSDLREPAFLLNRGKTPESGRIFGEIVAGRLPGLVHVETSSGTV
                     YCWNRGDAALAEEIAIRTGCDLVHARSAAKPQDGLREIRGCIPGEAVFVNGIVIGTAA
                     ADTVVLAMEDGSLKAVSGLDPKPHGFEKLLRSGLPDITRAWCKSGPVRSAPPRQGSRV
                     CRGGRVAVVDHCGHTLYTAIGDDVCGVLAIGDDTTAVCGHICSHAGIPVFGVVDGDAD
                     AIVEPGYAPGSVVVEVIDGRDDDLGREIADGRDLQASTWEDWVEETLREIDGRVRILL
                     DLRER"
     gene            complement(19559..20248)
                     /locus_tag="PK196_02065"
     CDS             complement(19559..20248)
                     /locus_tag="PK196_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HON40243.1"
                     /translation="MHYEITGDNLQMVTIRLAPGESVCAEAGAMVNMSGNMKMTTNVK
                     GGLFKGLKRVVTGESLFMTEFTPEKGEGFVAFAGNVPGKIFTLDLDGREFISQKDAFL
                     CSEPGIDLDIAFTKKLRAGAFGGEGFILQRLSGSGKAFLHCCGDIKEMTLAPGEVVKV
                     ETGLVVGFDSTVDYSIALAGGVKTVLFGGEGLFLTTLTGPGRVILQSMDLAKLASALI
                     PFLPTQNSSGR"
     gene            20422..20709
                     /locus_tag="PK196_02070"
     CDS             20422..20709
                     /locus_tag="PK196_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HON40244.1"
                     /translation="MGSGRAGQGMVDPRDVPVGDVYITLEEVRIVLDRQGRRCVRCGA
                     PLRPGRTYFDLRRPVICGGSRTPGNVEALCPSCHRRHMRRIRELLAMHQRR"
     gene            20739..20951
                     /locus_tag="PK196_02075"
     CDS             20739..20951
                     /locus_tag="PK196_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40245.1"
                     /translation="MTCMLRLAAALFQGSAPPDGWRSMTMRRLDAALTPGTPENLAAA
                     LALAAITVPWSRAAAGFVLGSIRRGG"
     gene            complement(20958..22727)
                     /locus_tag="PK196_02080"
     CDS             complement(20958..22727)
                     /locus_tag="PK196_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HON40246.1"
                     /translation="MTGRQPEFLPMTPEGRDRFEIILVTGDAYVDHPSFGTAIIGRVL
                     VDAGYTVGVIAQPDWRTETDLRRLGEPSLFFSISAGNVDSMVNAFTPNLKRRRSDVYS
                     PGGRLLRPDRATLVYTDRVHALFPDTPIVIGGIEASLRRFAHYDYWSDTVRQSILADA
                     PADLLVYGMGERQVVEIAGRIAAGEPLTGIPGTAHRLDLAAWRSADHSGCIVLPSYDD
                     LRSDRHAYARAFAMHYAEQDPIRGRPVAQPHPKTVIVQNPPAMPLSTAELDHVYELPY
                     TRRAHPSYTEPIPALDPVRFSIVSHRGCFGGCSFCALTHHQGRIIQSRSIDSIVREVE
                     RMTGMPEFRGVVQDIGGPTANMYSLRCSRWETAGTCPDRRCIDCPSLDRSHREQLRML
                     QRVSEIPGVKRVFISSGIRYDLIPPDDDYLARICARHISGHLKVAPEHISERVCACMG
                     KPPRQVFDAFRERFDALQKDRQRRQYLLPYFMSGHPGCRIEDMIELAVYVRDTGLYTE
                     QVQDFTPTPMSISTTIYHTGLDPFTMEEVYVPKGREKKIQRALLHYRDPKNYSLVREG
                     LLAAGRADLIGNAWNCLIPAKRR"
     gene            complement(22724..>23196)
                     /locus_tag="PK196_02085"
     CDS             complement(22724..>23196)
                     /locus_tag="PK196_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HON40247.1"
                     /translation="SEELIDPMQYSEGKVGRVFTVRIDDGKDFLQEIQRFVTAMAIQS
                     GTIQFLGAVRSARLVTGPKEPVIPPSPHGEEIFGGWELIGFATIYPGDDAPSIHLHTA
                     AGKGMRSLAGCLRDRAEVYLVIEAIITEFIGITAKRIHDEKTGVNLPVFDRTLQ"
BASE COUNT         4031 a         7273 c         7566 g         4326 t
ORIGIN      
        1 ctgctctcag ggcaaaacct gatagcacaa atgcgatagt agtctagcgg caggacaggg
       61 gcttcccaag cctctaaccc gggttcgatc cccggctatc gcatccctgt tttggtttga
      121 tcgttctggt gcgagatggc cccgcatttg cgagagatcg cagatctttg cataatggca
      181 gcactgagaa aatccaaaaa ccacgttgtg ccccctccct gaaaaggggc gccatttttg
      241 tgagaaccaa cccgctggga aaaaatgacc gggtttttta cccccggcga catcgtgtcg
      301 cgatcaggat gagcagcgcc gccccggccg ccgccgcacc gaacccgggt gcggtcgtcg
      361 tcggcaccgg ttcttccgtc tcttctccag gctccgtgat ccctgccagc caggcggtgc
      421 tgttgccggt ggatatgatc tggatacccg ccaggacata accgccgcca ggagcgctgg
      481 cggcggcata cacccggctc ccggggaacg tcatcttcca gagtatggcg ccgtcagcgg
      541 ccgtcccgaa gacaaaaccc tccccgctcg ctgcgttcgt acctgcaacc aggtagccgc
      601 cggagggctg gatgccgaca gcgtaaccgg tgagcccctc catcttcctg gcccaggcct
      661 catctccgtc ctgatcgacg agcccatacc agtagttccc ggagtagaca aacccgccgt
      721 ccgcggtctc ctcaaggtcg aagatgaccg gaacctggta gttcctggac cagagggtgt
      781 tgccgtcggc atctagccgg atgaggaacc cgtcccccac atcgtaggtg aagggcgatg
      841 acgttccgcc gacgaggcag ccgccatcag aggccacaat cgcggtgttc accgcggtgc
      901 ccgggaacgt cctgttccag atgggtgtcc cgttcccgtc ggtcttcacc agaaccccgg
      961 tcgtctcgtt ctcagagccc gccgggttcc agagccagcc tgcggcaaga tacccgccat
     1021 caggcgtctt tgcaaccgac gagaccttca tacccgggag agcctcccgc cactcctcct
     1081 cacccgcggc gtccagcctg atcaggaacg aggtgccctg gtattcggcc cccccatccg
     1141 tctcgttgat cgagacgctg tatacaccgg cgatgcaccc gccgtcagcg gccgccgcaa
     1201 cggaggctgg cgccatcccg ggcagccttt tcagccagac ctggttgccc gccccgtcgg
     1261 tctttacgag catcacgtcc tcctccccgc cgctggttct tgacagggtt gaaccgatgg
     1321 cgatgatacc cccgtctgca gagggggcaa cggcatcaaa cttgttctca ttctccagcg
     1381 taaacgtcac gttccaggcg gcctctggag aggccgcatc tgcggaggcg acccccgccg
     1441 gcagcagcac cgcaatgaac agtgctgcca ggatcgcttt ccttcgccag aagccttgag
     1501 ggttttctgt catggagatc catgaagggt ggggcggcac gcccaaaaag cgtagtggtt
     1561 cgccgccggc aatcccttaa tgaacgtgga cttccaataa ttctagcacg tgctggcgac
     1621 attcgacgga acctgggtgc acctgaagag cgaggggcgc acgctctacg accagggcgg
     1681 ttacgggaga ccggagaaag acggtctttg cctctccccc gaagaagccc tctacctcat
     1741 ggagcgaaac aagatcgagg tgaaggacct cgactttgat gccctgctcg gtctcttcgc
     1801 cgaccggcca aacttcatcc gcaggtacat ggtctaccgc gacatccgcg agcggggcta
     1861 cgtgattcag cctggcccgc acgatttccg agtgtttcgc cgcggccaca aacccggatc
     1921 cggaaagtcg cgatacctga tccgggttct ctcggagcgt gatctcatcg atttcgaccg
     1981 cctggatcag gatgcgcttg ccgcggtcaa catgcgaaaa cagtacctcc tggcggtcgt
     2041 cgatgacgag gacgaactga cctactacga ggtacggctg caggacctcc caccgctcag
     2101 caaccccccg gcctgtgagg cgcctgtgga ggcaaggctc cttggaacat acgcgcttgc
     2161 ccacctgccg ccgggaaacc ggcttgagga ggagtggtat ggcaagaggc ttgatgccga
     2221 acgcctcctc tttcgccccg tcgagtcgat ctacctgatg cgcagacgct gccttgagat
     2281 ccatcacgac ggcggaccga tgaccgcaga gaccctcctt gacgccgttg cggaggttga
     2341 tatcgagatc cgcgagaaag agcgggtctt ttccgacctc cgggacaaag gctacatacc
     2401 caggaccggc tacaagttcg gccaccactt ccgtgtctac tccgggaaga agagccactc
     2461 cgagatgctt gtccacgccg tcccggcagg aacttccatg ccgatgagcg ccgtctcccg
     2521 ctccgtcagg ctcgctcaca gcgtcaaaaa gaagatgttg tttgcctgcg tacataacac
     2581 cggtatcagg tacgtcgaat ttggcagaat aaaactgtga gcgtcattac atgaattctg
     2641 agataaaccc ctggtcaaac aaccagaccg tcgatctgga tcgactcttt tccgagtttg
     2701 gtatcgagcc tattggtgag gtggcacgaa cccttccaga gatgccgccg ttcatacgcc
     2761 gcggcatcgt cgtcgggcat cgggattata acctgatcgc cgacgctatc cggaacaaaa
     2821 cgccgtttca tgtccttacc gggttcatgc cctccggcct cccgcacctc gggcacctga
     2881 tggtcatgaa agaggtcgtc tggcacgtgc agcagggcgg gacgggctac gtcgccatcg
     2941 ccgaccggga ggcgcacgcc gtccgcggca tatcctggga gaagtgccgg gactacggga
     3001 aggagtacct caaggccctc tacgccctcg ggttcgaggg cacgacctac taccagagcc
     3061 gaaacgagcg tttgaaagac ctggccttcg aggcgtcaac gcgggtgaac ttctcggaac
     3121 tcaatgcaat ctacgggttt ggcccggata cgtctctctc tcacgccatg agcgtcgtca
     3181 cccaggtggc cgacatcctc ttcccgcaag tggatgccgg tccggcgccc acgatcgtcc
     3241 cggtcggcct tgaccaggac cctcacctcc ggctaacccg ggacgtggcc tacaaactcc
     3301 ggatgttcac ggtcgaggac cgcggcgagt acatcagcgt ccggtcaaag aacgcccctg
     3361 aagaggccat tgaggcggtt catcgcgcgt tcccgggctc gaagaggtat gcggcccatg
     3421 tggatatacc gggtctatcg cagagccagg tggaggagac cgtccggcgg atcgagatcg
     3481 atcacggcgg gttcgggttc taccgcccct cctcgaccta ccacatcttc atgccggggc
     3541 ttcagggcgg gaagatgtca agcagcgtgc ccgcgagcct gttcgggttc tacgagcccg
     3601 actcctcggt aaagaagaag gtcatggcgg cgcttacagg cgggcggatg acgcttgagg
     3661 aacagaggcg tcttggcggc gatccggact cctgcccggt ctacctcctg aacctcttcc
     3721 acatgcttga tgacgatatc gaacttgccg acctgcgccg ccgctgcgag agcggtgaac
     3781 tcacctgcgg gcagtgcaag aaagagaccc tggaacgcgt gcgggcgttc cttgcggatc
     3841 tgcgggatcg gatggatgcg gttgagcacc ttgccgagga ggtatgatac ggtggaactg
     3901 acgcagaacg agaagaaact cctggttgcc ctgggagagt ccgggtcggc cgatgcgacc
     3961 agccttgcgg agaagatgaa cacccgcccg gaggcggtgg tgcagtatgc gaacctcgcc
     4021 cgtgagcggg ggcttgtcga ggttgagaaa cacgtcaccc ggcgctacag gccgacggag
     4081 gaggggctgg cctatgtcgg gaagggtctc cccgagcggc agttgttcga gagtttcggg
     4141 gaggctatcc ctatgcgcga cctccagaaa cacccgcttg caaagatcgg gatcggctgg
     4201 atgcgcagga aaggctggat caccatccgc gacggtgtgg tgcataagac cggcaacgct
     4261 ccacctggcc cggacgaggt cgcgtttgcc cggcttggag agaccggcga gatccccgcc
     4321 ggcgaggagg gtgttctcga tctcgtgaaa cgtgggctca tagaggagga ggaggccgtc
     4381 aactacacca tctcgctaac accagagggg agagccctcc tggccgcggg ggttgacctc
     4441 cgggaggagg tcgggacgct cacacgggaa cagatcatct ccggcgcctg ggagagcctc
     4501 cccctccggc gctacgacgt cacaaaactg ccgcgccgcg tctacccggg aaagacccat
     4561 ccataccagc gcctcatcga tgagatgcgc cgtgtcctct ttgatatggg gtttgccgag
     4621 atgtcgggcg ggatagtcca gagctcgttc tggaactttg acgccctctt ccagccccag
     4681 gaccacccgg ccagggagat gcaggataca ttcttcctgg ccgagcgctg gcctcttccc
     4741 gagggttacg agcgcgtccg cgagatgcac gagcacggcg gcgatacctc gtccaccggc
     4801 tggggaggga ggtggagcgc cgagaaggcg cagcagtgtg ttctgcggac gcacacgacc
     4861 agcctctcga tccagcacct ggcggagcat cgggaacccc cggtgaaggc gttctgcatc
     4921 ggccgggtct accggcggga ggccatcgac cccacccacc tggcggagtt tgagcagctt
     4981 gagggtatag tgatggacga gtctgtaaac ttccgtcatc tcctggggtt cttgaaggag
     5041 ttctacgcga agatggggtt tgataaggtt cggttccggc cgggctactt cccctacacc
     5101 gaaccatccg tggagcctga ggtctacgtc gagggtctgg gctgggtgga gcttggcgga
     5161 gccgggatat tccgcgaaga ggtgacggcg ccattcggga tcgagcaccc ggtgcttgcc
     5221 tggggtctcg ggataagccg ggtggcgatg ctgcggctcg ggctgcggga cctccggcac
     5281 ctctacagga gcgatgttga gtggatccgg gagacgccca tctacggcgg ggggcggtga
     5341 gagtggcgat cataacgctc ccctacaggt acctggaacg gctgacgggg gttgaccggg
     5401 agacgatcat cgagcgcctt ccgatgatag gtgccgatat cgagcgggtc gaggatgacc
     5461 acgtggatgt tgagttcttc cccgaccggc ctgacctcta ctcgccggag ggtgtcgccc
     5521 gggcgatgcg ggggttcctg gggatagagg cggggctgcc ggtctacagc gtccgcccct
     5581 cgggtataac cttctctgtg gatccggggc ttgccgatat ccggccgtac ctgggctcag
     5641 cggtgatccg gaacgtcaac ctcgatgaag agtcaatcga gtcgcttatg gggctccagg
     5701 aggccctcca ctgggcggtc ggccgcggcc gaggcaaggt ggcgataggc gtccacgacc
     5761 ttgacgctgt caggccgcca ttccactaca tcgcctcgcc gcgggatcgg tcgtttgtcc
     5821 cgctggactt tgagagggag atgacgatgg aggagatcct ggtcgaccac ccgaagggcc
     5881 gggattacgc tcacctggtg gagaacttcc cgcggttccc gctgatcgtc gatgccgatg
     5941 accgggtgct ctcgtttccg ccgatcatca acggcgaggt tacgcgggtc acggcggcga
     6001 cgaagaacat tcttctcgac tgcaccggta ccgaccggaa agcggtgatg acggcggtaa
     6061 acatcatctg cacggcgcta ggggaggcgg gggcgagcat cgagtcggtc aacgtggatg
     6121 gggaggagat gcccaccctt gcacccgcgg aaaggctggt ctcggttgag gactgttctt
     6181 ctctcctcgg catccctctc actccgggag agatggcgcg gctgcttggc cggatgaggt
     6241 ttggcgctga acctgcaggc gattcacggg tcctggtccg gatcccctgt taccgggcgg
     6301 acatcctcca cgactgggat atatttgagg atgtggcaat cgcctacggg tttgagaact
     6361 tcgaggccac cctgccggcg gtctcgacca tcgctgcgga gcacccggtc acgacgatag
     6421 agagggcggt tcggtcggtg atgacggggc ttggctacat cgagatgata ccgttcaccc
     6481 tcacgaacga gagggtgctg tatacgaaca tgcagcggcc tccgttgccg gggacgctga
     6541 ggctgctcca cccgatcagc gaggagcaga cggtggtgcg gaccgacctg ctgccgctgt
     6601 tgatggaggc gctgtttgca aacaagcatc gtgacctgcc gcagcggtta tttgcggtgg
     6661 gcgatgtggt ggaggactgt ctgacctacc agaaggcggc ggcggtcagc atccacccgg
     6721 cggcggactt ttcggaggca tacgcggcgg cggacgtcct gtgccgggag gtctcgatcg
     6781 gctacactgt cagggagtcg gccgacccgg cgttcatcga tggccgccgc ggggatatag
     6841 ttctcgatgg aagggttgtg ggggtgttcg gggagatcca cccggccgtg ctgcgggcgt
     6901 tcgatctcga gcacccggtt gcggcgctgg cgctcgatct cagagccgta ccgggatacc
     6961 ccgctccgcc aggtactcct tcacctggcg gacggtgaac tcgccgtaat ggaagacgct
     7021 tgccgccagg caggcatcgg ccttcccttc tgtgaaccct ttgtagaagt gttcgagcga
     7081 acccacgccg ccgcttgcga tgaccgggat cccgaccgat tccgagacgg cgcgggtgat
     7141 tgggatatcg aatccctccc gggtgccgtc ggtctccatg cttgtgagca ggatctcgcc
     7201 ggcgccgcgc tcttcggcct ccatcgccca tttgacggcg tcaagccctg tgggtctgct
     7261 gccgccgtgg gtcacgacct cgtaccagca ctcccggccg tcggcgaggg tgatcggcgt
     7321 cctgccgggg gccatatcgt agttgcgccg gacgtctatc gcgacgacga tgcactgggt
     7381 gccgaacctc ccggcgccct ttgtgatgag gtcggggtcc ttgacggcgc ttgagttgat
     7441 gctcaccttg tcggcgccag cccggaggat ctgtttcatg tcttcgatgc tccggatgcc
     7501 gccgccgacg gtgaggggga ggaagagttg atccgccgcc cgctgcacca catcgatgat
     7561 ggtgccccgg tcctcgcggg tggcggtgat gtcgaggaag accacctcgt cggcgccctg
     7621 ctcgttgtag cgccgggcga gttccacggg gtcgcccgca tcctgcagcc cgacgaagtg
     7681 cgtgcccttg acgacccgtc catctttgag gtcgaggcag gggatgatcc gcttcgttaa
     7741 aaccatgggt actggttatt gagcgtgagt tgatgatggt tttgttctgc ggaaggggac
     7801 gatggcagac gttccgggcg gcatggctat acagggggga tgtctccaac ccccccacgg
     7861 ggcgattctc catcgatacc gtgccacggg ggacgagcag atctctgtcg tcaatgtgaa
     7921 ccgcctccct ggagacggtc accgagtcag tcgccaaaca ctcgccagcc ctctctggag
     7981 cggccgggag gtgagggtgg ctcgacgaga gaggctttca ccgacttgcc aagacggggg
     8041 ggggcgatcc atcatggata cggcgggcag ggaggacaac tctggcgtcc cgccgaacac
     8101 ccccttcacc gcaattcaat ttttcccctc ccccctctgc acggcttccg ggcccatcac
     8161 cacccaccgt ctgcctccct caccccgcac cccatttcct gccgctgtag ccctccccgc
     8221 caataacccc cctatgttta agtccttcgg gagaatactt ttagagacaa accggaggag
     8281 catccggcgg gattaaccgg tctggtggtt tttatggagt ctggagatct taagatagca
     8341 tgggcgcggc agtacatgcc ggtactctcg tccatccgga accgtttcgt tgaggaaaag
     8401 ccgttctccg gcatgacaat cgggatggcg ctgcacgtcg aggcaaagac cgcggtgctc
     8461 gtcgagacgc tggcggcggg cggcgccgag gtgcacatca ccgggtgcaa ccccctctct
     8521 acgcaggacg atgtatccgc cgcgctcaac acccgcaagg ggatccactc ctacgcccgc
     8581 cggggcgccg gggtggagga ctactacgcg gccatcgacc gggtgctcga cgcacggccg
     8641 gcgatcacca tcgacgacgg catggacctc atccatcgcc tccacacgga gcgccgggag
     8701 atgctggaga cggtcatcgg cggctgcgag gagaccacga ccggcatcca caggcttcgc
     8761 gcgatggcgc gggatggggc gctcatgttc ccggtcatcg ccgtcaacga cacccccatg
     8821 aaacgtttct tcgacaacgt ccacggcacc ggcgagagtt cgcttgcatc gatcatgatc
     8881 accacaaacg tcctcatcgc cggcaaacgc ctcgtcgtag cgggatacgg ttactgcggc
     8941 cggggagttg ctcagaaggc caggagcctt ggcgcccgcg tcatcgtgac cgaggtcgac
     9001 ccccggaggg cgctccaggc gcacatggac gggtttgacg tcatgacgat ggaccaggca
     9061 gcgccgctcg gcgacctctt cgtcaccacc accgggaaca gaagcatcat aacggaacgc
     9121 cacttcccga agatgaagga cggtgcaatc cttgcaaacg ccgggcattt caacgtggag
     9181 atcgatctcg actggctcgc ctcgcacgcc gactccaccg tccgccgcga cggcatcgat
     9241 acctacctcc tggacggaaa ggcggttcac gtcctcgccg agggcagact tgtgaacctt
     9301 gccaccccca agggcatggg ccacccaatc gaggtgatgg acctcagttt cgccgtccag
     9361 gctctctccg cccgctacat cgcgaagcac ggccgcgaac tcaaacccgg tgtccacgac
     9421 gtcccatcag agatcgacga ggaggtggcg cgactgaaac tcgatgcact cggtctctct
     9481 atcgaccggc tgacacccga acaggaggcc tacatgaact cctggacgat cgggacgtga
     9541 cccggtcttt tgaccttttt ttttgccggc cgtgtcgaaa ccttaacgct tctccccgcc
     9601 gacccttctt ctcatgtggc aggcgctgga tatcgaggcc tggattgccg gccggcggtc
     9661 tgacctcgac cgggtcaaag aacccgtctc tcagatcatc gcaagggtgc gggcggaagg
     9721 cgacgccgct ctcctcgacc tgacgaagaa gtttgacggc atcgacctcg aggagatcgc
     9781 cgtaccgaaa gaggagctgg gggcggccta cgaccgcgtg gatgcccggc tggttgagag
     9841 cctggtggag gcagaggcgc gcataagcgc gtttcacgag ttgcagcgcg ggcgcgacct
     9901 ctggctccag gagatggagc ccgggatcac cctcggcgtc aggacgacac ccctatctcg
     9961 tgtgggggca tacgtccccg gcgggcgggc ggcatacccc tcaaccgccc ttatgtgcac
    10021 cgtaccggca aaggccgccg gcgtatctga gatctgctgc tgcaccccgc cgccggtcag
    10081 cccgataacc ctggtcgccc tcgacatagc cggggtggac gaggtctacc gtatcggcgg
    10141 cgcccaggcc atcgcggcga tggcaatcgg gacagagacc gtcccccggg tggagaagat
    10201 cgtcggcccc gggaacgtct acgtcaccgc cgcgaagatg ctcctccggg acgaggtaga
    10261 gatcgacttc ccggcaggcc caagcgagat cgccgtcctc gccgacgaaa cggccaaccc
    10321 gacctttatc gccgccgata tcctggccca ggccgagcac gaccccaacg ccgcctgcgt
    10381 cctcgtgacc acccgccccg gtctcgccga tgccgtcggc gccgaggtgg accgtatggt
    10441 cgccgggtca gaacgccgcg agatcatcat gaaagcgctt gagaactcgg gttatgtcct
    10501 ggtatcagac ctcgacgagg gcgtggcgac gatcgatgcg atcgctcccg agcacctctc
    10561 gatccaggtc tccgacccgc ttgcggtctt aaaccgcgtc aggaacgccg ggtctatctt
    10621 tgtcgggccc tacgccgctg tggcctgcgg cgattacgcc tccggcacaa accacgtcct
    10681 ccccacagcg ggctacgccg ccatatactc cgggctcgat ctcaaccact tctgccggcg
    10741 gtcgaccgtc caggttataa gccgtgaggg gcttttagcc atcgccgacg ttgtggagac
    10801 gatcgccgat gccgaagggc tccacgccca cgccgaatcg gtgcgggttc gaaggaaggg
    10861 gtgagcggag gtctctcttt gaggggcgat tccactccat gaccggtttt tgccggttct
    10921 gcatgtattt tatctctgtg tggtgaggtg atccccatgc cggacgagaa gcggaaggcg
    10981 gaactgaagg tcaccgggat gcactgtgcg tcctgcgccc tcaacgtgga gcgtgccctc
    11041 acggcccggg agggcgtcca tgacgcccgt gtcaacctgg ccaccgggac tgccagcgtc
    11101 gactacgacc ccgcaaagac cacaatcgcc gatatcgagc aggcggtcgc cgatgccggt
    11161 tacggcgtcc tgaagagcac cgccaccgtc aggatcggcg ggatggtctg tgcctcctgc
    11221 gccagcgttg ttgaggcctc gctcaccggt ctcgatggcg tctacgacgc ccgggtcaac
    11281 ctcgccgccg agaacgctta tatagtctac aacccggaac tggtcacgat cgctgatatc
    11341 cgcgccgcca tcgaggatgc cggttacacc tacctggggc tcgaggagga gatccccgcg
    11401 gatatcgagg agaagatgcg cgaggaagac ctccgcgaga agttccggcg gttcaccatc
    11461 gggtttgcgg tgagcgttcc gctgtttgtc atcatgctcc tcggcatacc cgatatgctc
    11521 gctctccctg tctcctttaa cctgatcctg ctcatcatca ccctgccggt cttcgtctac
    11581 gtcagcgccc cgatcttcaa ggctgccgct gcggcgctgc gccaccgggc gctgacgatg
    11641 gacgttatgt acgccatggg catcggtgtc gcctacggtg caagcatact cgggactttt
    11701 gaggttctcc tcaccccgga gttcgccttc tacgacaccg ctgttatgct ggcctcgttt
    11761 ctcacgctcg gccgatacct cgaggcccgg gcaaagggga agacgtctga tgccatcagg
    11821 agccttgtag gcctgcggcc caagaccgcg acggtcatcc gggacggccg ggaggtcgag
    11881 gtcccggtcg aggcggtggt ccccggcgac atcatcattg tccggcctgg agagaaggtg
    11941 ccggtggacg gggttgtcgt tggcggggag agttcagtcg atgagtcgat gatcaccggt
    12001 gaacccatac cggtcgagaa gcgggagggc gacgcggtcg tcggcggaac cctgaacgtg
    12061 aacggcgtcc tccgcgtccg cgctgagcgg gtggggaagg agatggtcct tgcacagatc
    12121 atcaggcttg tccgggaggc ccagggctca aaaccgtctg ttgagcggat cgccgacgtc
    12181 gcggtctcct acttcatccc ggccgttctt gcagtcgcca ccgccgcgtt ccttgtctgg
    12241 tacctcgtcc tcgacgcctc tctcctcttc tctctcacgg tgctgatctc cgtcctggtg
    12301 gttgcctgcc cctgtgcgct cggtcttgcc accccgaccg ctgtgaccgt cgggatcggc
    12361 cggggcgcag aactcgggct cctcatcagg aacggcgagg cgctcgagat atcggagaag
    12421 ctgaccacgg tcgtcttcga caagaccggg acgctcaccc gcgggaaacc ggacgtcacc
    12481 gatatcgtcc cgttaggggt tgcggaggag aggcttctct cgattgctgc ggcggtcgag
    12541 tcaaactcgc agcacccgct cgcggcggcg atcgtgcgcc gggcggagag cgcgggtctt
    12601 gaccttccac cttcggagcg gttcacgaca ttcggcggcc tgggtgtgag cgcccttgtg
    12661 gagggcgcgg aggtgctgat cgggaacccg ccattcctca ccgagcacgg cgtcaccctc
    12721 ccgccggagg cagcggagag gatcgccgcc ctccaggacg aggggaagac cgtggtcctc
    12781 gtcgccgtcg gcgggacgct tgcgggggtc atcggcatcg ccgacaccct caaaccgacg
    12841 gcaaagagcg ctgtttcaga actgaagagg atggggcttg ccgtgatgat gctcaccggc
    12901 gacaacgagc ggacggccgc cgcgatcgcc cgggaggtcg ggatagagaa cgtccgtgcc
    12961 gggttgatgc cgcaggagaa ggtcgaggag gtccgcgccc tccagcagcg gggcgaggtt
    13021 gtggccttcg tcggcgacgg gatcaacgac gcccccgcgc ttgcgcaggc ggacgtcggg
    13081 atcgccatag ggagcgggac ggatgtcgcg atagagagcg gggatatcgt cctgatccgc
    13141 gacgacctga ccgacgtggt cgcggccatc caggtctccc ggaaggtgat ctcccggatc
    13201 aggcagaacc tcttctgggc gtttgcctac aacgccgctc tgatccctat tgccgcgggt
    13261 ctgctctacc cattcttcgg gatcaccttc cgcccggaac ttgcggcgct tgcgatggcg
    13321 ctctcctcgg tgacggttgt ctcgctctcc ctgatgctca agacttatat acctccggca
    13381 aagaaactgg aggttgatac agatggcagt tgatccggta tgcgggatgg aggtcgatga
    13441 ggcggccgcc aggttcaaaa ctgagtacag gggcaggacc tactacttct gcgccccggg
    13501 gtgcaagaaa ctcttcgagc gcgaccctga ggcctacctg aagaagccgt gatccctttc
    13561 cggcggcgca atcagaacct gtagaccgcg ccgccgcacc tcccgccgtg ccgccccttt
    13621 cccggggcga ggtcggtggt atcgtcgcag acgccgcgga ggacagcact tgcgttgtcg
    13681 gcgaagtgga cagtccaggc ctccggtgtc cggggctgga tctcgccgtg gggtccgtgg
    13741 tgcgacatga tgatgtggag gatgtgcgcg aaccgtgcag ggtcgacgcc gtcggcgtgc
    13801 tgcatgaccg cggcggcccc gatcgcggtg tgcccgatca gtgtgtattc ggggagagcg
    13861 gcaaacgaga acccctggcg ccggaaacag gaggctttcc cgacatcgtg gaggagggcg
    13921 ccggcgagga tgacatcggt atccatcgcc gcccgcctga cgcctcccgc cagtgcaacc
    13981 gcgttctccg cggtctcaag ggtgtgctcg gcaagcccgc cggtgcaggc gtggtggcgc
    14041 cgtttcgctg ccggggcctc gaagaacccc ggcgtctcct caagaacccg gccgacgagg
    14101 gaggagatgc cggtgtcgct gatcatcgcg gccatgcgtg ccagcccctg gcggacggcg
    14161 gcggtgtcta cgggggcgta gacgaagagg gagggctcga tcccctccag gggcagaggt
    14221 tctgccaggg agaagatgcc ctcgttgacg ttgacctcgc acgtgccgtt gtagaccttc
    14281 gcgtagcccg cgatgcggta gacctctccc ggcctgatac cgcggcagaa cgcctctatc
    14341 tcttcggcgc tctggctgcc tgtcccccag accttgcagg ctccccggcc gctgcggtcg
    14401 gatatggtga ggtggatgta cttcccgccg tccttcttct cccggatttc tgccgaatcc
    14461 acaacgaatg gtgcgtcgac cgggacaccg ccggttatct ccccgacaaa gacattctta
    14521 tcgaccacag tggccagcac tcctgagaga gttctgtcgc ggacggcact taaaacccgg
    14581 tggtctgttt gccggcgagg gtcaggctga ccccgtctga cccttctgcc gggcgtaacg
    14641 ttcggcaagc gagtccagcg tctcgacctc gtccaggctc ttgtcctcac gcacccggac
    14701 aaaccggggg aaacggaggg catacccgct ctcgtagttc gggctggtct ggatctcgga
    14761 gtaaccaacc tcaaagacca cacctggctc gaaagtgacc tcttttcctg atcgggcaat
    14821 cacccgatcc ttgaagaggt cgtagaggtc tgccagaact tcgtctgtga tcccggtcgc
    14881 caccttcccg accgggagga gccggccgcc ctcgtcctgg acggcgagca ggaacgaccc
    14941 gaacatcccg gccctccggc cctctcccca ctcggccccg atcacggcga ggtcgagcgt
    15001 ctcgacgccg ggcttaacct tcacccagag ccggccgcgg acgccggggg tgtaggggga
    15061 gtccaggacc ttcaccatga ccccttcgtg gccgagattc agggcgtcgg cgtagatctc
    15121 ctccgccctg gccggatcgc ctgtccggaa ctgcggggcg acgtggtcgt ggaggctctc
    15181 ctcgaggtat cttcgccgct ctgagagcgg gcggtcgacc agcgactcgc catcgaggta
    15241 gaggatatcg aagacctggg ggacgagttc tatctgctcc atcatagagt cgatctcgtg
    15301 tttccgccgg aaccgccgga tcacgtactg gaagggcatg ggtctcccct cgcggacggc
    15361 gattgcctcg ccgtccagga tgacgtcatg atcggtagat tccatgaggc ggcgtgcgac
    15421 gtcggggatg ctctctgtga cttcttcgag ttttcgcgag tagatccggc acctgccgcc
    15481 gaccttgtgg aactggaacc ggcttccgtc gtatttgtac tcgacggcga cggtgccgtg
    15541 ttcggcgatc tggtcggcga tggcgccagc ctgcgcaagc atcatcttca ccggccgaaa
    15601 cggcctgatc ctgacgcggg agagttcttc gggattgttc cgggcgagga gggcgacctc
    15661 cccgaggtcg tttataacct gctgggcgtg ctcaaccagg tggacatcta caccgaaagc
    15721 ccgggcgatg gcgtcgcgga cgttcccttc gcccatgcct atccggagct cttcgagcat
    15781 aagtctggcc aggtaacgcc cctctatcgg gcgggcgttg ccaaagagcc cctctgccac
    15841 ccggagtttc tcccgctgcg accgctgccc ttcggcggcc gccatacgtt cgaacccccg
    15901 gtagacgtcg aggagttcga gttcctggat gaagaaagag gtctgttctt tctttgccag
    15961 gagttcctcg accgcaagcc ccacgtcgcc tgttctgttg accgcttcaa caactgtctc
    16021 tcgcttcacc cctgcaacgt aggccacggc gtcgtagagg aggtttggac ccacgccgag
    16081 ttttctggtg ctccagtcgg ggaagatcct gcccatgatg aacctgacga agatggggag
    16141 gtcggtctcg tcaagtccgg ggaggacggt ggcgacctct tcgatcatct ccaggcgtcc
    16201 ggagattcct tcaaggtgtt cgcagatgcg ggcgaactcc agaaactgca tacccatatc
    16261 ccttctgtag taactttccc ggtggggaga atcaaccctt ctgtccggtt ctgcatgatt
    16321 gatgtggttg tgatccctaa accttactga tgcagcagga tgaagaactc aaggatatgc
    16381 tgcgcgacct catctggctc aatgccctga tcgcgaccga actgatccag atcacggaga
    16441 acacgtccgg gatcctccgg cgggcgccgc cgccggagtc gtgtatcgct gaacatgcgg
    16501 ctctccgggc gacggcgctc gatatcgccg accgctatcg gccggggacg atgctccggc
    16561 agcacgttga gaagcacagt tgatgccggc cgggtctgct gcggctccca aacactcaat
    16621 aactggatga acagatccag tgatcggtga ggttagatga aactcctgat tgcgattgca
    16681 ccggaacggt tcagggatga ggagctggat gtcccgaggc gggcgtttct ggaggccggg
    16741 atagatgtgg atatcgcctc aacggtcacc gggaggtgca gggggatgct cggcggcaca
    16801 gcggcggcgt ccctgacgtt tgacgacgtc aatcccgacg actacgccgg gatcgttctg
    16861 gtgggcggaa gcggctctcc agactacctc tgggggaacc ggaaactcca gagccttgtt
    16921 cgggcgttct ttgagcaggg caaggttgtc gctgccatat gtctctcgcc ggtcgtgctt
    16981 gcgcgcgccg ggatcctggc cgggcggcag gcgacggtct acccgagccc ggacgccata
    17041 cgagagatga agaaggcggg ggcaaacctc gtcagcgtcc ccgtcgtcgc cgacaggcag
    17101 gttgtaaccg ggaacggtcc tgaggctgcg gcacagtttg ccgatgccgt catatctaaa
    17161 ctggagtgct gagcgggggc ggcgttgttg agacccgggt tgtgggggtg ttttcagtgc
    17221 cctgcctgaa aagatagcgg gcagtgagac gggagcgatt cgccatcgat gccgtgtccg
    17281 ggaggagcat ctccggcggt tacggtgaaa ccctccccct gcacggcctt ccgggccact
    17341 cgccgcccca gctcagaaat caaacagcgt cttctgcgtt ctcagttccg ggatgaggcg
    17401 gctgtagggc tgccagtggg agaacccgac cagccttgag gccaggtcga ccgcctggtc
    17461 gaggctggcg cacggcgtcc cggggccgcg catagcgttt ctcgcggctt cccggacgac
    17521 ccaggtgccg agcggcgccc agtactcgcc tgtgacgtgc cggaggacca gaacccgtgc
    17581 ggaccgctgc acgccgtcca ggtactccgc caccgccagg cgtgccgagt agtaggcccc
    17641 cgcaagcggc gagtagccgg attttatcag cccctcctcg ccgtcgcagg cgatcgtctc
    17701 gtccccctgc ccccagagcg actgcctgcc ccagacctcg atcatctcga acttccagtc
    17761 gcccggaacc aggatgcaga cgatatggtt gccatacagc gatgccgaga agaccctgat
    17821 cccatcgagc ggcgggtaac gcacaatctt tttcttgagg cgttttgaga cggtgtcatc
    17881 gaccgccgtg atcgcccagc gtgtcgggac gatatgccgt ttcttctcgc ctccaagcaa
    17941 cccggcggtg aggagctggg ttatccggta gacgtcggtc ccggaggagt ggaggaggcc
    18001 gcaggcctct gtcgcggaga ggtcggtgtc cgacgtcacc cggtcgaccg gtctctccac
    18061 ccgggggttg tccaggacgt ccaggtgccg gatggcgccg gtgaggccta tgggtgcgat
    18121 cgtcccgtcg aaccggagat cgaaagtgac gggttttaca aactgcgcct cgacatcgag
    18181 cggtctgctc gagagcgcta tctcctggat actcccggca acgctctttg actcgccggc
    18241 gccgcggatc gtccgcgccc gaagcccgac tatatcgtcg atctggaggc cccgcgcaac
    18301 ccagtccggc gggttgtcgg tgtcgtcgat catcagcggc cccgccgaaa ccttcgggta
    18361 agcgtaactc ccgacaaaga ccgagggcgc cgtgccctgg tagtggtcgc tcggcctgac
    18421 ctggagcctg gcgtagaacc ggctcatgac ggggcacctc tcaagcccgc agagcccttt
    18481 tcctttgcac tcggcacagc gcatcattca gcgctcccgg aggtcgagga gtatacgcac
    18541 ccttccatcg atctcgcgca gcgtctcctc cacccagtcc tcccaggtcg aagcctggag
    18601 gtctcttcca tctgcaatct cccggccgag atcgtcgtcc cggccgtcga taacctcaac
    18661 caccaccgag cccggggcat accccggctc cacgatggcg tcggcgtccc cgtcgaccac
    18721 gccgaagacg gggatcccgg catgagaaca gatgtgcccg cagacggcgg tggtatcatc
    18781 accgatggca agcactccgc agacgtcatc cccgatcgcc gtatagaggg tgtggccgca
    18841 gtggtcgacg accgccaccc tcccgccgcg gcagacccgg ctgccctgcc gcgggggtgc
    18901 agaacgcacc ggcccgctct tgcaccaggc cctggtgata tccgggagac cggagcggag
    18961 gagtttctca aacccgtggg gtttggggtc gagaccggag accgccttca gcgacccgtc
    19021 ctccattgcc aggacgaccg tatccgcggc tgctgtccct atgactatcc cgttgacaaa
    19081 gaccgcctcc ccggggatgc acccccggat ctcccgcagc ccgtcctgcg gtttcgccgc
    19141 gctcctcgca tggaccaggt cgcagcccgt ccggatcgcg atctcctctg cgagagcggc
    19201 atcgccgcgg ttccagcagt agacggtgcc gctcgaggtc tcgacgtgga cgagccctgg
    19261 gagtctcccg gcaacgatct cgccaaagat ccgtccggac tccggggttt ttccccggtt
    19321 gagcaggaac gcgggctccc gcagatctga aagcaccctg ctcgggcgct cgtccgtgca
    19381 ggtgaccggg agcccggact cctcggcggc ggttcgggcc atcaccccgg cgacgagcac
    19441 ccgccgcggg cagaggagcc ccatcaaccg ctcgacgtcg ccggcatcga aggccgcggg
    19501 gccgtgcacc accatgaccg cgttcattgg agagattggg actttgcggg gtatcaggtt
    19561 accgtcccga ggagttctgg gtggggagga aggggatcag ggcgcttgcg agttttgcga
    19621 gatccatcga ctgcaggatg acccggccgg ggccggtcag cgtggtcagg aagagcccct
    19681 cgcccccgaa gaggacggtc ttcaccccac cggcaagcgc tatgctgtag tcgacggtgc
    19741 tgtcgaaccc gacaacgagc cctgtctcca ccttgaccac ctcgccgggc gcaagcgtca
    19801 tctccttgat gtcgccgcag cagtggagga acgccttccc gctcccggag agccgctgga
    19861 ggatgaaccc ctccccgccg aacgctcccg cgcggagctt ctttgtgaag gctatatcga
    19921 ggtcgatccc cggttccgag cagaggaacg catccttctg ggagatgaac tctctcccgt
    19981 caaggtcgag ggtgaagatc tttcccggga cgttcccggc gaaggcgaca aacccctccc
    20041 ccttctccgg ggtgaactcg gtcatgaaga ggctctcccc ggtcacaacc cgcttgagcc
    20101 ccttgaagag tccgcccttc acgttggtgg tcatcttcat gttgccgctc atgttgacca
    20161 tggcgccggc ctcggcgcag acgctctcgc cgggggcgag gcggatggtc accatctgga
    20221 ggttgtctcc cgtgatctcg tagtgcatgc tgatgagtat tgaatcgggg aagaatatcc
    20281 tttcccctct gcggggagtc ttgttgaggg tatcaaaccg gtaattcccg ggtcctgggg
    20341 ctggcggtgg ggatggtcga tgaaaggcgt ttaccgccgg tgcggcacaa cgcttatcac
    20401 ctggaagggg catctctccg catgggatcc gggcgcgcgg gacaggggat ggtggatccc
    20461 cgggatgtgc ctgtgggcga tgtctacata acgcttgagg aggtgaggat agtccttgat
    20521 agacagggcc gccgctgcgt ccggtgcggc gctccgctcc gtccggggcg gacctatttt
    20581 gacctccggc ggccggtgat ctgcggcggc tcccggacgc cggggaacgt tgaggctctc
    20641 tgcccctcct gccaccggag gcacatgcgc cggatcaggg aactgctggc catgcaccag
    20701 aggagataaa taatcctccg tgcatacctg ccgtctgtat gacctgcatg ttgaggctgg
    20761 ccgctgctct cttccagggt tcggcgccgc cggacgggtg gcgctccatg acgatgcgcc
    20821 ggctcgatgc cgccctcacc ccggggacgc cggagaacct ggcggccgcg cttgcgctgg
    20881 cggcgataac cgtcccgtgg agccgggcgg ctgccggttt tgttctcggg tcgatccggc
    20941 ggggcgggta acctcctcta ccttctcttt gccgggatga gacagttcca ggcgttcccg
    21001 atcaggtcag ccctcccggc ggcgaggagc ccttcccgga cgaggctgta gttcttcggg
    21061 tcgcggtagt ggaggagagc ccgctggatc ttcttctccc ggcctttcgg gacgtagacc
    21121 tcttccatgg tgaaggggtc gagcccggtg tggtagatgg ttgtcgagat gctcatcggg
    21181 gtcggggtga agtcctggac ctgctcggtg tatagcccgg tatcccggac gtagactgcg
    21241 agttcgatca tgtcctcgat ccggcacccg gggtggccgg acatgaagta gggaaggagg
    21301 tactgtcgtc gctgtctatc cttctggagg gcgtcgaacc gttcccggaa ggcgtcaaag
    21361 acctgacggg gaggttttcc catgcaggcg cagacccgtt cagatatgtg ttctggagcg
    21421 accttgaggt ggccggatat gtgccgggcg cagatccggg cgaggtaatc gtcgtccggc
    21481 gggataaggt cgtagcggat gccggaggag atgaagaccc gtttcacacc cgggatctcg
    21541 gagacccgct ggagcatccg gagttgttcc cggtggctgc ggtctagcga ggggcagtcg
    21601 atgcaccggc ggtcggggca ggtgccggcg gtctcccagc ggctgcagcg gagggagtac
    21661 atgtttgccg tggggccgcc gatgtcctgg acgactccgc ggaactccgg catccctgtc
    21721 atccgttcaa cctcgcggac gatcgagtca atgctccggc tctggatgat ccggccctgg
    21781 tggtgggtga gtgcgcagaa cgagcagccg ccgaaacagc cgcggtggct gacgatggag
    21841 aacctgacgg ggtcgagcgc gggtatgggt tcggtgtatg aggggtgtgc ccgccgggtg
    21901 tatgggagtt cgtagacgtg gtcgagttcg gcggtcgaga gcggcatcgc cggcgggttc
    21961 tggacgatga cggtctttgg gtgcggctgg gcgacggggc ggccgcggat ggggtcctgt
    22021 tcggcgtaat gcatcgcgaa tgctctggcg taggcgtggc gatcggatct gagatcgtcg
    22081 taggagggga ggacgatgca gccggagtgg tcggcgctcc gccatgcggc gaggtcgagg
    22141 cggtgggcgg tgcccgggat cccggtgagg ggttctccgg cggcgatgcg gccggcgatc
    22201 tcgacgacct ggcgttcgcc catgccgtag acgaggaggt cggcgggggc gtcggccagg
    22261 atggactggc ggacggtgtc tgaccagtag tcgtagtggg cgaaccgccg gaggcttgcc
    22321 tctatcccgc cgatgacaat gggggtgtcg gggaagaggg cgtggacgcg gtcggtgtag
    22381 accagggttg cgcggtctgg ccggaggagt cggccgccgg gggagtagac gtctgagcgc
    22441 cggcgtttga ggttaggggt gaatgcgttc accatcgagt caacgtttcc ggctgatatg
    22501 gagaagaaga ggctgggctc gccgagacgc ctgagatcgg tctcggtgcg ccagtctggc
    22561 tgggcgatga cgccgacggt gtagccggcg tccacgagca cccggccgat gatggcggtt
    22621 ccgaaggagg ggtggtcgac gtaggcgtcg ccggtgacga ggatgatctc gaagcggtct
    22681 ctcccctccg gggtcatggg gaggaactct ggctgccggc cggtcattgg agtgtccggt
    22741 caaagacggg gaggtttacg ccggtcttct cgtcgtggat ccgttttgcg gtgatgccga
    22801 tgaactcggt gatgatagcc tcgatgacca ggtagacctc tgccctatcc cggaggcaac
    22861 cggcgagcga gcgcatcccc ttccccgcgg cggtgtggag gtggatggag ggggcgtcgt
    22921 cgccggggta gatggttgca aacccgatga gttcccagcc gccgaagatc tcttcgccgt
    22981 ggggggatgg tgggatgacc ggctcttttg gcccggtgac gagtcttgcc gaccggacgg
    23041 cgcccaggaa ctggatggtg ccgctctgga tcgccatcgc cgtgacaaac cgctggatct
    23101 cctggaggaa gtctttcccg tcgtcgatcc ggacggtaaa gaccctgccg acctttcctt
    23161 ctgagtattg catgggatcg atcaactctt ctgata
//
LOCUS       DAOSZX010000012        22773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1864_length_22773_cov_20.485298, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000012 DAOSZX010000000
VERSION     DAOSZX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22773
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_22773_cov_20.485298"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..994
                     /locus_tag="PK196_02090"
     CDS             452..994
                     /locus_tag="PK196_02090"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HON40248.1"
                     /translation="MFVPSKCFFTKGVGIHKDKLASFELALRQAGIEKYNLVYVSSIF
                     PPNCRLISKEAGLQEISPGSIVYVVMARNETNEPSRLVSAAIGHAMPRESDAYGYLSE
                     HHAFGETEEVSGEYAEDLAATMLATTLGIEFDPDKAWQEREQIYKASGRIISTTHICQ
                     AAEGVMDGRWTTVVAAAVFL"
     gene            1098..2387
                     /gene="aspS"
                     /locus_tag="PK196_02095"
     CDS             1098..2387
                     /gene="aspS"
                     /locus_tag="PK196_02095"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HON40249.1"
                     /translation="MRLPIRAVTPETGSAEIVGWVHEIRDLGGLSFFLIRDRTGIIQV
                     TIPKKKVTAEILETARSVSRESVVRVRGRVKAIDKAPGGREIVPEELEIINLAESPLP
                     LDVVEKVPADLDTRLDSRFLDARKPRVRAIFFIRSAVICAATEFLSSRGCINITTPKI
                     VAAATEGGTELFPIAYFEKEAFLNQSPQLYKQMMMAAGFEAVFEIGPIFRAEEHNTIR
                     HLNEATSLDIEISFADHNDVMELLEDLIVHIYGSVAKNCADDLAEIGVDLAIPERPFP
                     RIPYLEAIEIANQSIEEKISFGDDLTPAAERAIGDTMGGHYFIVDWPTDIRPYYAMPY
                     PDRPAFCKAFDLMHPRMELASGAQRIHDHDLLVERIRAKGLSPESFEFYLKTFRYGMP
                     PHAGWGLGIERLVMTMLDLPNIREAVLFPRDRHRLTP"
     gene            2443..3345
                     /locus_tag="PK196_02100"
     CDS             2443..3345
                     /locus_tag="PK196_02100"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HON40250.1"
                     /translation="MTLTAMHLPTTVVGSYPVVKGSGLMARVDPLKHAVEVAVGDQIA
                     AGIDIVSDGQVRGDMIRAFTSHLPGIRGAAVVGKVQPARQPITLADTKYALSRHPKVK
                     GILTGPSTIAYGLSIETTFYRNREELILDLAQALAVEAEHLQNAGVALIQIDEPIFST
                     GAANLTVGREAVNAIAGRLRVPVCLHVCGNLGDVIDEILRVNVEIFDFEFSNNPHNLE
                     IISKKDLHGRMIGYGCVDSADPGIESVETIHKRIEMGIEVFSPDVMLIDPDCGLRMQS
                     REAASGKLKNMVAAARLARAEHPD"
     gene            complement(3342..>3943)
                     /locus_tag="PK196_02105"
     CDS             complement(3342..>3943)
                     /locus_tag="PK196_02105"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HON40251.1"
                     /translation="NRGIDVAGSTKRSTGIAGLDLALDGGFPAGARIVISGSPLSGLE
                     LLARQFWQEGGGTGAYLMLDALPADGMVDARGMSLEEMVSAMTGERIIVDSLSTLIVT
                     LGIEAATRFVVEDSSALIGDGSNIVYLLYANHHTAFEEARVMRAADIFITLRQEIHGN
                     EVERTLAIEKFRDEDVPRRVIPYNIMAEGIEVSTTSRVF"
     assembly_gap    3944..4043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4122..5708)
                     /locus_tag="PK196_02110"
     CDS             complement(4122..5708)
                     /locus_tag="PK196_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HON40252.1"
                     /translation="MPNCTTFLDANASNPSKVALIVPSTGESYTRAELLDQVSRVGRG
                     FQEIGITPGERVCIYLDSSTEYLLSYLALWRIGAVAVPTNRVYRENEVLYALRDAGAA
                     AVITDVDGMAVVGRIREHVPGLRHIIAVGGGEESGAATPWSDLLRSPPGLRAVNCRFD
                     DICQIQYTSGTTGRPKGAMLTHGNWIAAMDAERDVLGITDRDVYLGIYPMGHVGISWG
                     ISALRAGATYIVMERFDLDRYIELARKYRATIVAGMPPVIHSLLQTPPGTEEALATVR
                     AMISGGGPLTPGIWKPFHERFRIPIVNAYGLSETIVVGTGTAIRPEHYATADEFRSVG
                     TPVGFSEVKIVDANDSARELRAGEDGEIALRGPAVALGYWQMPEETADVFLPDGWFLT
                     GDIGHLDENGMLYLTDRKKDMIVMSGWKVYPTEVEDVLLQHPGVRDAAVFGCADEHRG
                     EVPVAVVIPAGNGVTPEDIIAFARERLAGYKVPRRVIFVDEIPRVNGWKLLRKRLQED
                     YCDACIQGGRSYGGVQLLRQ"
     gene            5889..6152
                     /locus_tag="PK196_02115"
     CDS             5889..6152
                     /locus_tag="PK196_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HON40253.1"
                     /translation="MKSNSADHTGITTLDRLEPGCRSRIIGIRAFGSLRKHMLAMGMV
                     PGAEVEVIRAAPLGDPVEYRVKGYCLSLRRAEAGLVEVALEGQ"
     gene            6149..6394
                     /locus_tag="PK196_02120"
     CDS             6149..6394
                     /locus_tag="PK196_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HON40254.1"
                     /translation="MMESVCPLAFLAPGTVARVAWFRAGKGLQTRLFGMGFCPDVRVK
                     VVSADRGSVIVSVGGARYALSRGAAMKVMVRGIEESA"
     gene            6391..8385
                     /gene="feoB"
                     /locus_tag="PK196_02125"
     CDS             6391..8385
                     /gene="feoB"
                     /locus_tag="PK196_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HON40255.1"
                     /translation="MKREIRIALIGNPNIGKTTIFNLLTGSRQHVGNWPGVTVEKKTG
                     RLSRNGYEIEVVDLPGTYSLTAYSPDEIIARDFILEGNPDVVVQVVDATNMERNLYLT
                     LQVAELGVPMVIALNMVDLARAQGDGIDHERLSASLGIPIVPTVGTSGEGVDELLDAA
                     IRVAEGSPNHKLTVDYGSGVEVMIARLADALTTDTVLVDRYPLRWLAVKLLEGDENVR
                     AKVCGGPANGRVEEILSSIDTDDCEARMADKCYEKIDTILPGVRKTSTGRRVNPSDLV
                     DSVVTDRYLGIPIFLALMWGAFELTFTVGAPFAAAIEAGTAHLAELAAGSVESTNLAS
                     LLGDGIIGGLGAVLVFLPNIFILFLVLAILEDSGYLARAAFVMDRPLYALGLPGKAFI
                     PMLIGFGCNVPAIMAARSIENENDRLLTILANPFVSCSARLPVYILLAGTFFPEHAGS
                     VVFFLYVLGIAVAIGSAKLFRSTILPGEVSPFVMEMPPYHLPTVKTTLICTWSRGSMY
                     LRKAGTIIFGGVVIVWLLASFPPGVEYGSAGSLAGTIGHLMEPLVAPLGFDWKIAVAL
                     IFGFIAKEIVVGSLGTLYGTGEETLPAALLADPALSAATALALMVFALLYTPCAAALG
                     VIRKETGSWKWTGFSVAYGLTVAWVLAFVVGHVAAFVWGA"
     gene            8388..8828
                     /locus_tag="PK196_02130"
     CDS             8388..8828
                     /locus_tag="PK196_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40256.1"
                     /translation="MNDKIKTIFGAAAGALYTASGTLQFVWAIFGSLGGLEVLHISGD
                     LFNGFVLLVIGAVLLTGAWKVSTGAMEGVSFIYVGLFLSVLFGAVALCSTVAGAIEAT
                     FFAGEGESAWSAVDAVSPLLYLAATGAIGLLAWGREFFRGPPAS"
     gene            8825..9166
                     /locus_tag="PK196_02135"
     CDS             8825..9166
                     /locus_tag="PK196_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40257.1"
                     /translation="MMRKIAERIAGGGATIRSLAADLDVEPDLLLERLFMMERLGFVE
                     RSGLCSEPSGTGTFSCPHSTGCPGCSMIRGDGGLLYTLTEKGRRLAGANDPEGAWIPG
                     QSRKETTKTVD"
     gene            9373..11007
                     /locus_tag="PK196_02140"
     CDS             9373..11007
                     /locus_tag="PK196_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON40258.1"
                     /translation="MAGIIILALILTVVHPTSAGIYTDSCGMVWTDTCVPFERNNETA
                     YNEELAASFAPIAANLTEPPPDFSNISWSDAFRETCAYMEERYAFTDWRAVDWNSLYS
                     TYAPGIAEAEKNGDKAEYYRTLREFVYAIPDGHVLVRAAEDFGAKYADIGGGYGLAIA
                     RLDTGEVIVTYVANGSEAERTGIRFGDEVVSWDGRPIGDAINATPIIWTPIKPSTAEG
                     VLLHKQRYLTRAPVGDVATIGIVSQTDSLLHMVNLTAADDGYDTLSRTSIFLGREAND
                     GKAGSSADLETILTDEIVTSRTLPGGVVYIAVYEEVYDAYQPFTTAVKDAIEDGAPGI
                     VIDLRFNRGGDDNLASAFAGWFVDRPVFYEYGTFYDPGTGKPAVLWESWTQPRPNRYD
                     GPVAILVSPYTISSGEGLPKVFAESGTGAIVSWYGTNGAFGIESIGPTLPLGIVTAFP
                     MGASLDENGRIQVDSNASLIGGVAPTVRVPLDRDTLARAMAGEDVQLAYAVDWLEAEA
                     MAGGAERSPVPTQEAAAAGVAPLAALAFIVVWHGRR"
     gene            complement(11097..13514)
                     /locus_tag="PK196_02145"
     CDS             complement(11097..13514)
                     /locus_tag="PK196_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HON40259.1"
                     /translation="MPEIYLKVDSAYPEDQGAGKARLDPDTMLHLRLNPGDLVAIEGK
                     RRTVAKVWRAMVNDWHQGKVRIDNFTRLNAGASIGDRVRVRTLDEEIEAKRVVLAPPE
                     DLPKHLPVNYGSVVNRLIDFPVVRDDSVPIQVGLPFMQPQIVAFKAVVVEPENAVIIT
                     RNTKIEFSEKPAAGFEGVRRISYEDIGGLKDELQRVRETIELPMRHPEIFRKLGIEPP
                     KGVLLYGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQRLREVFEDARQH
                     APAIIFIDELDSIAPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRIDAID
                     PALRRPGRFDREIEIGVPSEDDRSQVLQIHTRGMPLADDVLIEEIARQTHGFVGADLA
                     ALAREAAIKALRRYLPEIDLETDEIPAEILEMMEVQARDFREALREVGPSAMREILLE
                     VPHVSWEDVGGLEEAKRDVREAVEYPLTRREQFENLGIEPPKGVLLYGPPGTGKTLIA
                     KAVASESGANFVPIKGPQLLSKWVGESERAVREVFKKARQVAPSIIFFDELDALTPAR
                     GGGTESHVTESVLNQILTEIDGLEELRGVVVMGATNRPDMVDPALLRPGRFDRLVYIG
                     EPGREDREKILSIHTRYMPIEGSTMDELVEMTGGFSESDLEDLVLAIGANRQVSLDEI
                     REHLAAITPDDKEGLRPYLRRKKIVELFSREKITLDDPVRSHLVRRVAAETEGFVGSD
                     LEALAREAAMLAMREGAAVVSQTHFDRARERVHATMNERVREYYQKIRHHFKGGLPKE
                     TQPLEYQ"
     gene            13561..14757
                     /gene="larC"
                     /locus_tag="PK196_02150"
     CDS             13561..14757
                     /gene="larC"
                     /locus_tag="PK196_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HON40260.1"
                     /translation="MNVVLFDPFNGAAGDMVIGSLLDLGADEEAVRAAMRSVVGEPTI
                     ERVDRCGIRAVQVMTHATAEHRTLDEVLDRLAGSDAPKEALEMARKVFLRIHRAEESI
                     HGAGAHFHEVGADDAIADVVGACTALHSLAPDGVAVLPLALGRGFSVGAHGIFPIPAP
                     ATVAILAESHLQMVPGGEERELCTPTGAALLSEFSTIRPDELGPASIRAVGYGAGSRN
                     PAERPNVLRAMLLETEEDNRGSDRIDILETNVDDVTGEMLTYTLGRLMEEGARDATAI
                     PVLMKKGRSGHTVRVISPPAATGHLTAVMARELGTLGIRCLPMVHRAVAERTIERVKV
                     RFGGQEWWIDVKCGWSEGRITSIKAEYEQVARCARETGLPFREIAARAEATARRLFIE
                     QGVLDP"
     gene            14754..15431
                     /gene="radB"
                     /locus_tag="PK196_02155"
     CDS             14754..15431
                     /gene="radB"
                     /locus_tag="PK196_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HON40261.1"
                     /translation="MKTERVSTGSQALDGLLGGGLERGAITQIYGEPASGKSVFCLMA
                     TVATLKAGNSVVYIDTEGFSVERFAQIAGGDAGELAERLYLFEPLDFTQQGAMIADTE
                     ALLKKAGKAPVDLVVMDSATALYRMELDLGREALRKLSHHMIKLLGLARKYEIPVLIT
                     NQIYMDVERDRVAGLGGTALEHLSKAIVRLERRDSVRRAVLLKHRSRPEGLSFDFAIT
                     GEGIRTL"
     gene            complement(15418..16236)
                     /gene="dapF"
                     /locus_tag="PK196_02160"
     CDS             complement(15418..16236)
                     /gene="dapF"
                     /locus_tag="PK196_02160"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HON40262.1"
                     /translation="MEITFTKLHGNGNDFILIDEFDQEIIPEEMKGTFAALYCDRRFG
                     IGADGVLYLQPSDKADIRMRLFQPDKSEAAMCGNGIRCLAKYAYDAGYVREPCTVETG
                     AGIAPVRMGYADDEFSATITMPDPAFERSAIPAVGKGDYREEICGFTVYAANIGVPHA
                     VIFVDDIDSVEIEIFAPPIRHHPTFPEGANVNFAEKVAFDKIRIRTFERGVEGETASC
                     GTGATASAAVARRLGLVGDTVQVETRGGPLVIRFGETVTMEGPAETVFSGVITF"
     gene            complement(16326..16398)
                     /locus_tag="PK196_02165"
     tRNA            complement(16326..16398)
                     /locus_tag="PK196_02165"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16362..16364),aa:His,seq:gtg)
     gene            16675..18456
                     /gene="infB"
                     /locus_tag="PK196_02170"
     CDS             16675..18456
                     /gene="infB"
                     /locus_tag="PK196_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HON40263.1"
                     /translation="MANQSAIRTPIVCVMGHVDHGKTSLLDRIRGSSVVSTEEGAITQ
                     HIGATLVPIDAIIRMSGALSRVNINVPGLLFIDTPGHHAFTTLRARGGALADMAIVVV
                     DINEGFRPQTIEALQILRNYKTPFVIAANKIDRIHGWRVNEGQPFLKTFAQQNERVQG
                     VLETRVYELVGKLSDLGFNSERFDRVSDFARNICIVPTSALTGEGIPDILMMLIGLAQ
                     RYMTGSLRVTADGPGAGTVLEVKEERGLGMTLDVILYDGTLRVGDDIVVAGNDQIIET
                     KVRSLLKPRPMSEILVEERFERVRSVTAAAGIKVAAPKLAGVIAGSPLRAVRGGNRDE
                     VIEEVRHEVQDIQVKLSDTGVIIRADTIGALEALSKELDSHQIPVMRAVVGPVTRHDV
                     IEAGTIRDPLYSAIIAFNTPVLPDAIDALADTSMSHVSIFEGGVIYQLIDDYIEWREE
                     KKQELERQRFEKVIMPARIRILPNCIFRQSNPAVVGVRVLGGKLQSGVDLVLPEGKKV
                     GRIKQIQERNETIQEAEAGKEVAISIDGPTVGRQINVDDELYVDIPERHVKVIEREML
                     DNLRPDLRETLEEFTAIKRRENPFWGK"
     gene            18547..18945
                     /locus_tag="PK196_02175"
     CDS             18547..18945
                     /locus_tag="PK196_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HON40264.1"
                     /translation="MAEFKIVLSDRETGRSYKVDATGPAAGALIGRRIGEEIEGGVLG
                     FAGYRIRITGASDRTGIPARRDLPGPARRRLLLSRGVGFHPVREGERRRKSVRGNEIS
                     ADIVQVNAVVTQRGSKPLEEYFNRPEAAAE"
     gene            complement(19113..19841)
                     /locus_tag="PK196_02180"
     CDS             complement(19113..19841)
                     /locus_tag="PK196_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HON40265.1"
                     /translation="MNGCWDGPIPEPQIRTIEDMRDVLADPGCTSDQPLYFMYRDLAR
                     SESDREWLRQHHIRYDITVIPARVICGEYVKTKGHYHPRNPAGEHYPEIYEVLKGRGH
                     YLLQTYPADDVVMIEASVGEKVMIPPGYGHVTINPGGEELVMANLVSSDFSSDYGPYI
                     ERRGAAYYEMEGGALVKNPRYPDAPPVRCCDPVEVPELGIRRETRLYDLIGQPRSVAF
                     LNRPEEFMGIMAGALRRVPQRGRR"
     gene            complement(19841..21121)
                     /locus_tag="PK196_02185"
     CDS             complement(19841..21121)
                     /locus_tag="PK196_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HON40266.1"
                     /translation="MRLQYIPILVLLLALLAVAPGSAQGVKYLYGSPDLSASIAGTNE
                     FAPGDETPVTVIISNSGLNTVKIAVPSMPPDDQPNTAKLATVALKSGDAPFTVKTDPQ
                     FVSDIRGGSSATATFNVKVADSAKPGTYTLPLDVTYTYIETVDDHTDVLRYNYIKKTV
                     TLPLEVRVTPDLRVEVLDVRTESLNVGTEGYVYMTIKNVGHDTGEKAIVKIARSGASP
                     LIPTDGSAYIGMLEPGETVDVKFKVSVADTAEEQSYPLDVAVTYENYEGETVTSKVVT
                     VGLPVGGKIDFEVVSPPTTLYLGQKSVLEVVYKNVGAAPVYSAQARISAVDPFTSSDD
                     TAYLGDLAPGETATARFEVNVDTDATEKEYGIDSEIRYRDALDNSKISDTMKVQVALE
                     RRQGGFFTNPIFIIVVLAVVIGAGYYIFVYRKKH"
     gene            21186..22004
                     /locus_tag="PK196_02190"
     CDS             21186..22004
                     /locus_tag="PK196_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40267.1"
                     /translation="MTASREVPATLIESLKSLGLTKYEALVYIGLLRVADATATEVHE
                     ISGVPRASVYPVLDHLVQKELVIVSHTMPKRFDAVPPAQGIENLMRRIRGDAESAREA
                     LEVFYREKELIDRGARELIWTIYGEENIKTRLADLFRSAEKSVEVLATRDLLHETVLP
                     LLESLPDAVEVEIVTAWQDEGNLPSRFRLRLLPLASSPGKLLPCERSGVFLVDDARAL
                     VWVGEVDSQPSALYSESGGLLLFVQRHITTVKEWARTTLNNRQGSPSSVYRSIP"
     gene            complement(21955..22701)
                     /locus_tag="PK196_02195"
     CDS             complement(21955..22701)
                     /locus_tag="PK196_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HON40268.1"
                     /translation="MHAPLKFTNHRIEDYALKVTYRPEENAGKVIYNLSVIESRDLEF
                     AISIMKEAHRQGITISDRLLISGPGDQVDGYTVAEGRNAICTICSITLDALLIQRGIP
                     LNPIGGGIVEIEGRIPRRFTCMILYRDTTLDPLEVLISQEITSILGVMRHGTGNILAN
                     IRECHMEAEHLLETALDELSGYGFSGILDVGAPNLPLLGVPVSPQYLGVAFVGGTNTM
                     AALREAGKPVVTRALKGLIDIQKMGYLDDY"
BASE COUNT         4583 a         6657 c         6815 g         4618 t
ORIGIN      
        1 tcctgaacct gatatatggg tgatcccacg cctcttcacc cccgctgtcc cccttccctc
       61 cagcagcgac caatcgggtg aaaagttcag ctggatccac cttaccggcc ccgcttgcag
      121 acagcgcgcg gctggcggca aacttcagga ataatacggt gctgaaacca ccactcccag
      181 ggcatcagat cctgacccac cctcgcagga tgaatgaacc tgccaggaca gaggttcggg
      241 catccatggg gatgaagcgg ccggtctgga ccgcgtcatc tccttttgcc tggggatggg
      301 ggcgtgattc atggctttac gcgtttattt tcttcatacg agactgaatt tcagccttaa
      361 gcagaaaaag aacagacatc caccgcattt tttgatttat ggcgtttata accgaaaata
      421 ttaaaaaatt caaacgataa gaagatctct gatgttcgtt ccgagcaagt gtttctttac
      481 aaagggcgtg ggaatccaca aagacaaact ggcatcgttt gaactggctc tcaggcaggc
      541 gggtatcgag aagtacaacc tggtttacgt atcgagcatc ttcccgccaa actgtcgact
      601 gatctcaaag gaggcgggct tacaggaaat ttctccaggc agcatcgtct atgtcgtcat
      661 ggcccggaac gagaccaacg aaccgagccg gctggtttct gccgccatag gccacgcgat
      721 gccgagggag agcgatgcct atggctacct ctccgagcat cacgccttcg gtgagaccga
      781 agaggtatcg ggtgaatatg cagaagacct ggcagcgacg atgctcgcca ccacgctcgg
      841 aatagagttc gaccccgaca aagcgtggca ggagcgggag cagatctaca aggcaagtgg
      901 ccggatcata agtacaacac atatctgcca ggctgcggaa ggggtcatgg acggccgctg
      961 gacgactgtc gtcgccgcag cggtcttcct ctgaaccctc gtttttcaag atcgaccatc
     1021 catctcttct actccccatt ttcagtaagc gcaagacaaa atacctgccg gggcctacac
     1081 tgtatccagg ttttcacatg cgactaccaa tacgcgccgt aacccccgag acagggtctg
     1141 cagagatcgt cggctgggtg catgagatcc gcgaccttgg aggactttcg ttcttcctga
     1201 tccgtgaccg gaccggtatc attcaggtga ccatcccgaa gaagaaggtt acagcagaga
     1261 tccttgagac cgccaggagc gtctcaagag agtctgtggt cagggtaaga ggcagggtta
     1321 aagcaatcga taaagcaccc ggtggacgcg agatcgtccc cgaagaactc gagatcatca
     1381 acctggcgga aagcccgctc ccgctcgacg tcgtcgagaa ggttccggct gacctggaca
     1441 cacgtctcga ctccaggttt cttgacgcaa gaaaaccacg tgttcgtgca atatttttca
     1501 tccgctctgc cgtgatctgc gccgcgacgg aattcctctc ctccaggggc tgcatcaaca
     1561 tcaccacccc gaagatcgtc gcggcagcta cggagggcgg caccgaactc ttcccgatcg
     1621 cctacttcga gaaagaggcg ttcttaaacc agagccccca gctttacaaa cagatgatga
     1681 tggctgcagg gttcgaggcg gtctttgaga tcggcccaat cttccgcgcc gaagagcata
     1741 acaccatccg gcatctcaat gaagccacgt cgctcgatat cgagatctct tttgccgacc
     1801 acaatgacgt catggaactt ctcgaggatc tgattgttca tatatatggg tccgtcgcaa
     1861 agaactgcgc agacgacctg gcggagatcg gggtcgatct tgcaatcccg gaaagaccgt
     1921 tcccgaggat accctatctg gaggcgatcg agattgcaaa ccaaagcatc gaagagaaga
     1981 tctcctttgg cgacgacctc accccggcag ccgaacgggc gatcggcgac actatgggag
     2041 gccattactt catcgttgac tggccaaccg atatccggcc atactacgcg atgccctacc
     2101 cggatcggcc agcgttctgc aaggcgtttg acctgatgca cccccggatg gaacttgcct
     2161 ccggcgccca gcgtatccac gatcacgacc ttctggtgga gcggatccgc gcaaaggggc
     2221 tatcacccga gagtttcgag ttctacctga agacgttccg atacggtatg ccgccgcatg
     2281 ccgggtgggg gcttggcatc gagcgtctgg tgatgacgat gctcgatctg ccaaacattc
     2341 gtgaggcagt gctcttcccg cgcgaccggc acaggctgac accatgaagt ttataatgcc
     2401 tggaactgat ggatcgatct attaggtgca gatgctgaga agatgaccct gactgcaatg
     2461 catctcccga ctacagtggt cgggagctac cccgtggtga aaggctcggg gcttatggcc
     2521 cgggtggacc cgctgaaaca cgcggtggag gtcgcggtag gcgaccagat cgccgccggg
     2581 atcgatatcg tatccgacgg tcaggtccgg ggggacatga tccgtgcgtt cacctcccac
     2641 ctcccgggca tccggggggc ggcggtcgtt ggaaaggttc agccggcgag gcagcccatc
     2701 acgctcgctg acactaagta tgccctttcc cgacacccga aggtgaaagg gatcctcaca
     2761 gggccaagca ccatcgcata cggcctctcg atcgagacaa cgttctaccg caacagggag
     2821 gaacttatcc tggatcttgc acaggcgctt gcagtcgagg cggagcacct ccagaacgcc
     2881 ggtgttgcgc tcatccagat tgacgagccc atcttctcca ccggagcggc aaacctcacc
     2941 gtcggccgcg aggccgtgaa cgctatcgcc ggtaggctcc gggtgccggt ctgtctccac
     3001 gtctgcggga acctggggga cgttatcgac gagatcctca gggtgaatgt tgagatcttc
     3061 gattttgagt tctcaaacaa cccgcacaac cttgagatca tctctaaaaa agacctgcac
     3121 ggccggatga tcggctacgg ctgtgtggat tcggccgacc ccggcatcga gagcgtcgag
     3181 acgatccaca agaggattga gatgggtatc gaggtgttct caccggacgt catgctcatc
     3241 gacccggact gcggcctgcg gatgcagtcg cgcgaggcgg catccggaaa actgaagaat
     3301 atggttgctg ccgcgaggct tgcccgggcg gaacacccgg actaaaagac cctgcttgtc
     3361 gttgatacct cgataccctc tgccatgatg ttgtagggta tcacacgccg cggcacgtcc
     3421 tcgtccctga acttctcgat ggcaagcgtc cgttcgacct cgttgccgtg gatctcctgg
     3481 cgcagggtga tgaagatgtc ggcggcgcgc atgacccgcg cctcctcaaa ggcggtgtgg
     3541 tggtttgcgt agaggaggta gacgatgttt gacccatcgc cgatcaaggc gctgctgtct
     3601 tcgacgacaa accttgtggc agcctcgatc ccgagggtga cgatcagtgt ggataacgaa
     3661 tccacgatga ttctctcccc ggtcatcgca gaaaccatct cttcaaggct cataccccgt
     3721 gcatcgacca tcccatctgc cggtagggcg tcaagcatca gatacgcgcc tgtgccccct
     3781 ccttcctgcc agaactgccg tgcaaggagt tcaagaccgc tcagcggcga cccggagatg
     3841 actatacggg cgcccgcagg gaacccgcca tcgagcgcaa ggtccagacc tgcaatacct
     3901 gtagaacgtt tggtgctgcc tgccacatcg atgccccggt tatnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnncctgcaa tacctgtaga acgtttggtg ctgcctgcca
     4081 catcgatgcc ccggttatat ccttgagagg atcctttgca gttattgcct taataactgc
     4141 actcctccat acgagcgtcc gccctggatg catgcatcac agtagtcttc ctggagacgt
     4201 ttgcggagca gtttccagcc gtttacgcgg gggatctcat cgacaaagat gacacggcgg
     4261 gggaccttgt atcccgcaag gcgctcccgg gcaaaggcga tgatatcctc gggggtaacc
     4321 ccgtttccgg cggggatcac cacggctacc gggacctcgc ctcggtgctc gtcggcgcac
     4381 ccgaagaccg cagcgtcgcg gacgccgggg tgctgaagaa ggacatcctc cacctcggtc
     4441 gggtagacct tccagccgga catgacgatc atgtccttct tgcggtcggt gagatagagc
     4501 atcccgttct cgtccaggtg gcctatgtcg cccgtcagga accagccgtc gggaagaaag
     4561 acatcggcgg tctcctccgg catctgccag tagccaagcg ccacagccgg tccccggagg
     4621 gctatctcgc catcctcgcc ggctcgaagt tctcgcgcgg agtcgtttgc atcgactatc
     4681 ttcacctccg agaacccgac cggggtacca acgctccgga actcgtcggc ggtcgcgtag
     4741 tgttcagggc ggatggcggt tcctgtaccg acgacgatcg tctcagagag gccgtaagcg
     4801 ttgacgatcg ggatcctgaa acgttcgtgg aagggtttcc atatgcctgg tgtcagaggt
     4861 ccgccgccgc tgatcatcgc cctgactgtg gcaagcgcct cctccgtccc tggcggtgtc
     4921 tggaggaggg agtggattac agggggcata ccggcgacga tcgtcgcgcg gtatttccgg
     4981 gctagttcaa tgtagcggtc gagatcgaac cgttccatga cgatataggt tgcaccggcc
     5041 cggagggcgg atatgcccca gctgatcccg acgtgcccca tcgggtatat ccccaggtag
     5101 acatcccggt ctgtgatgcc gaggacgtcc cgctcggcgt ccattgcggc gatccagttg
     5161 ccgtgggtga gcatggcgcc tttgggtctc cccgtagtcc cggaggtgta ctggatctgg
     5221 cagatgtcgt caaatcggca gttgacggcg cgaagtcccg gtggtgacct gaggaggtca
     5281 gaccaggggg ttgcagcccc gctctcctcc ccaccaccga ccgcgatgat gtgccggagt
     5341 cctggcacat gctccctgat gcggccgacg accgccatac catcgacgtc ggtgatgacc
     5401 gcagcggcgc cggcgtcacg aagagcgtag agcacttcgt tctcccggta gacccggttc
     5461 gtggggactg caactgcacc gatgcgccag agggccagat aactcagcag gtactcggtt
     5521 gaactgtcga ggtagataca gacccgttcc ccgggggtga taccgatctc ctggaacccg
     5581 cgccctaccc tggagacctg atcaaggagt tcggcacggg tgtaactctc tccggttgag
     5641 gggacgatta gagcaacctt tgaggggttg gaggcgttgg cgtcgagaaa tgtggtgcag
     5701 ttaggcatga gagaactcct agtgacatgt atgcacttta atgtatataa tcgttcatgg
     5761 cagtggcagg atgggttggc cccggttttc ggaattcgcc tgagatccaa aagatcctcg
     5821 acctggagac ttagatcctg aaatctttcg gatcggggtt atatcgaccg aataagagag
     5881 aatatccaat gaaatctaat tcagccgatc atacggggat aaccacgctc gatcgccttg
     5941 aaccgggatg ccgctcccgg atcataggca tccgtgcctt tggttctctc aggaagcata
     6001 tgcttgccat gggcatggtt ccgggggccg aggtggaagt gatccgggct gcacctctcg
     6061 gtgaccctgt agaataccgg gtgaagggct actgcctctc gctgcggcgt gccgaagcgg
     6121 gtctggtcga ggtcgccctg gaggggcaat gatggagagc gtctgcccgc tggcgtttct
     6181 ggctccggga actgtggcga gggtcgcctg gttcagggcc ggcaaagggc tgcagacccg
     6241 tctcttcggg atgggtttct gcccggatgt ccgggtaaag gtcgtctctg cggatcgggg
     6301 ttcagtcatc gtctcggttg gcggtgcccg ttacgccctc tcaaggggag cggctatgaa
     6361 ggtcatggtg aggggcatcg aggagtctgc atgaagcgag aaatcaggat tgcgctcatc
     6421 ggcaacccga acatcggtaa aacaacgatc tttaacttgc tcaccggttc aaggcagcat
     6481 gtcgggaact ggccgggggt gaccgtggag aagaagaccg gccggctcag ccgcaacggg
     6541 tatgagatcg aggtggtaga cctccccgga acctacagcc ttacggccta ctctccggat
     6601 gagatcatcg ccagggattt catcctcgaa ggaaacccgg acgtcgtcgt ccaggtcgtt
     6661 gatgcgacaa acatggagcg gaacctctac ctgacgctcc aggttgccga actcggggtg
     6721 ccgatggtca tcgccctgaa catggtcgat cttgccaggg cgcagggtga cgggatagac
     6781 catgagaggc tttctgcgtc cctggggatc ccgatcgtgc cgacggtggg aaccagtggc
     6841 gaaggtgtag acgaactcct tgacgctgcg atcagggtgg cggagggctc gccgaaccat
     6901 aaactgaccg tcgactacgg cagcggggtg gaggtgatga tcgcgagact tgcagacgcc
     6961 ctaaccaccg atacagttct tgttgatcga tacccgctcc gctggcttgc ggtcaaactc
     7021 cttgagggag acgagaacgt ccgggcaaag gtttgcgggg ggccggcgaa cggccgcgtt
     7081 gaggagatcc tctcttccat cgatacggac gattgtgagg cacggatggc ggacaaatgc
     7141 tacgagaaga ttgacaccat cctgcctggc gtcaggaaaa caagcacggg gcgacgcgtg
     7201 aacccatcag acctggtcga cagcgttgtc accgaccgtt accttggcat ccctatcttc
     7261 cttgcgttga tgtggggagc gtttgaactg acgtttacag tcggtgcccc ctttgccgcc
     7321 gcaatcgagg ccggcaccgc ccatctcgcg gaactggcgg ccggttcggt cgagtccacc
     7381 aatcttgcct cgttgcttgg agacgggatc atcggcggtc tcggagcagt cctcgtattc
     7441 ctcccgaata tctttatcct ctttctggta ctggcgatac tcgaggactc cgggtatctc
     7501 gcgcgagccg cgtttgtaat ggaccggccg ctctatgccc tcggtctgcc cggaaaggcg
     7561 ttcataccga tgctcatcgg gttcgggtgc aacgttccgg cgatcatggc cgcacggtcg
     7621 atcgagaacg aaaacgatcg gctgctcacg atccttgcaa acccgttcgt atcgtgcagc
     7681 gccagactgc ccgtctacat actcctggca gggacgttct tcccggaaca tgctgggagc
     7741 gttgtcttct tcctctatgt cctcgggatc gccgttgcga tcgggtctgc gaaactcttc
     7801 aggagcacca tcctcccggg tgaggtatca ccgttcgtaa tggagatgcc gccctaccat
     7861 ctcccgacgg tgaagacgac gctcatctgc acatggagcc ggggttcgat gtatctccgg
     7921 aaagccggta cgatcatatt tgggggcgtc gttatcgtct ggcttcttgc gtcgttcccg
     7981 ccaggtgttg aatacgggag cgccgggagt cttgccggca ccataggtca cctgatggaa
     8041 ccgcttgtgg cgccgctcgg ctttgactgg aagatcgctg ttgccctgat cttcgggttc
     8101 atcgcaaaag agatcgtcgt cgggtcgctc ggcactctat acggaacggg ggaggagacg
     8161 cttcctgcag cgctgctcgc cgatccagcc ctctccgcag ctacggccct cgccctgatg
     8221 gtctttgcgc tgctgtatac gccgtgcgcc gcggcgctcg gcgtcatccg gaaagagacc
     8281 gggtcgtgga agtggacagg cttttcggtc gcctacggtc taacggtcgc ctgggtcctt
     8341 gctttcgtcg tcgggcacgt tgccgcgttc gtttggggtg cgtgagcatg aatgataaga
     8401 tcaagactat attcggagcg gcggctggtg cgctctacac agccagcgga acactccagt
     8461 ttgtatgggc catcttcggt tccctgggcg gcctggaggt tctccacata tccggggatc
     8521 tcttcaacgg gttcgtgctg ctagtgatcg gagcggttct tctgaccggc gcctggaaag
     8581 tctccaccgg cgccatggag ggtgtatcct tcatatacgt cgggctcttc ctctcggttc
     8641 tcttcggggc ggtagcgctc tgttcaactg tggccggggc aatcgaggcc acgttctttg
     8701 ccggggaggg agagagtgcg tggtcggccg tggacgcggt cagcccgctc ctttacctgg
     8761 cggcgacagg cgccatcggt ctcctggcgt ggggtagaga gttcttcagg gggccccctg
     8821 catcatgatg cgaaagatag cagaacggat tgccggagga ggcgccacaa tcaggtcact
     8881 tgcggccgat ctggatgtgg agccggatct cctgctcgaa cggctattca tgatggagag
     8941 actcgggttc gtagagcgaa gcgggttgtg cagcgaaccc tccggcaccg ggaccttttc
     9001 ctgcccccat tctacggggt gcccgggctg cagcatgatc cggggagacg ggggcttgct
     9061 atacaccctg acagagaaag ggaggcggct tgcgggggca aatgatcctg agggggcctg
     9121 gatacccggc cagagtcgga aagagactac aaagacggta gattgaagtg tgcaccgagg
     9181 ggggcccggg ataagagccg ctcccgaccc cgggctcacc cccccgggtg cattgcagat
     9241 cctggtgagt ccacgcagcg gaaagccggg tagccgccgg gactccaata agaaagcgta
     9301 agcctcaaat cacgtcatgc tgaaagggtt cacgaaagga tcattatgtt tttcaggcac
     9361 tcgacccgga gattggccgg gattatcatt ctggctctta tccttactgt tgtacacccg
     9421 acgtccgccg ggatctacac cgattcctgc gggatggtat ggactgacac ctgcgttccg
     9481 ttcgaacgaa acaacgagac ggcctacaac gaggaactgg ctgcatcatt cgcgcccatc
     9541 gcggccaacc tgaccgaacc accccccgat tttagcaaca tctcctggag cgacgccttc
     9601 cgcgagacgt gcgcctacat ggaggagcgc tacgccttta cagactggcg cgctgttgat
     9661 tggaactcgc tttactcaac gtatgccccg gggatcgctg aagccgagaa gaacggcgat
     9721 aaggccgaat actaccgcac actccgtgaa ttcgtgtatg ccatcccgga cgggcacgtc
     9781 ctggtccgcg ctgctgaaga cttcggggcg aagtacgccg atataggagg tggctacggg
     9841 ctggccatcg cacggcttga tacgggagag gtcatcgtga cctacgttgc aaacgggagc
     9901 gaggccgagc ggacggggat ccggtttggc gatgaggtag tctcctggga cggccgaccg
     9961 atcggggatg cgatcaacgc aacaccgatc atctggaccc cgatcaagcc ctcaaccgcg
    10021 gaaggtgtcc tgctccacaa gcagcgatac ctgacccggg cgccggttgg agacgtggcg
    10081 accatcggga tcgtttctca aacggattca ctcctccaca tggttaacct gaccgcagcg
    10141 gacgacggct acgacaccct cagccggacg agcatcttcc tcggtcgtga ggcaaacgac
    10201 gggaaagccg gatcatcggc tgatctcgaa acaatcctta cagacgagat agtgacctcc
    10261 agaaccctgc ctggaggggt tgtgtatatc gcagtctacg aggaggtcta cgacgcctac
    10321 cagcccttca caacagcggt gaaggatgcg atcgaggatg gggcgccagg aatcgtcatc
    10381 gacctccggt tcaacagggg tggtgacgac aacctggcct ctgccttcgc cggctggttc
    10441 gtggaccggc cggtcttcta cgagtacggc accttctacg accccgggac cgggaagccc
    10501 gcggtcctct gggagtcatg gacacagccc cggcccaacc gttacgacgg cccggtcgcc
    10561 atacttgtaa gcccgtacac aatcagttcc ggtgaggggc ttcccaaagt ctttgccgaa
    10621 tccggaacgg gcgcgattgt ctcatggtac gggacgaacg gggcatttgg catcgagtcg
    10681 atcggcccga cgctccctct cgggatcgtg accgccttcc cgatgggcgc gtcgctggat
    10741 gaaaacggcc ggatccaggt ggacagcaac gcatccctca tcggcggggt ggcgccgacg
    10801 gtgcgggtgc cgctcgacag ggataccctg gcgcgggcga tggccgggga ggacgtccag
    10861 ctcgcttacg cggttgactg gctcgaagca gaggcgatgg ccgggggcgc ggagcgctct
    10921 ccagttccca cgcaggaagc agcggctgca ggggttgccc ccctcgcggc acttgctttc
    10981 atcgtggttt ggcacggccg gcgatgagtg gataggaggc cttctccttt tccggcttcc
    11041 gctggaacgg gaccgatcgc gctacaaaaa ggagttttcc gggtgccggg acacccctat
    11101 tggtattcga gcggctgggt ctctttcggc agcccgccct tgaagtgatg ccggatcttc
    11161 tggtaatact ctcgcacccg ctcgttcatc gtcgcatgca ccctctcccg tgcccgatca
    11221 aagtgcgtct ggctcactac cgcagcgcct tcccgcatgg cgagcattgc cgcttcccgt
    11281 gcgagcgcct cgaggtctga gccgacaaaa ccctcggtct cggccgccac cctcctgaca
    11341 aggtgtgagc gcaccgggtc atcgagggtt atcttctcgc gggagaagag ctcgacaatt
    11401 ttctttcgcc ggagataagg cctgagacct tctttgtcgt ccggcgtgat cgcagcaaga
    11461 tgctccctta tctcatcgag actcacctgc cggtttgccc cgatagcgag aaccaggtct
    11521 tcgagatcgc tctcggagaa gcctccggtc atctcaacaa gttcatccat ggtcgaaccc
    11581 tcgatcggca tgtaacgggt atggatgctg aggatcttct cccggtcctc ccgccctggt
    11641 tcgccgatgt agacgagacg gtcgaaccgc ccaggtcgca gcagtgccgg atcgaccata
    11701 tcagggcggt ttgtcgcccc cataaccacc acgccccgca gttcctcgag cccatcgatc
    11761 tcggtgagga tctggttcaa gacgctctcg gtgacatgcg actcggtgcc gccgccacgg
    11821 gctggagtga gggcatcaag ttcgtcaaag aagattatgg acggtgccac ctgccgggcc
    11881 ttcttgaaga cctcacgaac cgcccgttcg ctctcgccga cccatttgga gaggagctgg
    11941 ggacccttta tcggtacaaa gtttgccccg ctctcactgg cgactgcctt tgcaatgagg
    12001 gtctttcccg tccccggcgg gccgtagagg aggacaccct ttggaggctc tatacccaga
    12061 ttctcaaact gctcccgccg ggtcagggga tactccaccg cctcacggac atcccgtttc
    12121 gcctcctcca gcccgccgac atcctcccag gagacgtgcg ggacctcaag gagaatctcc
    12181 cgcatggcgc ttggccccac ctcgcgaagg gcctcacgga agtccctggc ctgcacctcc
    12241 atcatctcca ggatctccgc cgggatctcg tctgtctcca ggtcgatctc ggggaggtag
    12301 cggcgcaacg cctttatagc ggcctcacgc gccagggcgg caaggtccgc cccgacaaac
    12361 ccatgagtct gcctggcgat ctcttcgatc agaacgtcat ctgcaagcgg catgccgcgt
    12421 gtgtgtatct ggagcacctg ggatcgatcg tcttcagatg gcacaccgat ctcgatctcg
    12481 cggtcaaacc gcccggggcg gcggagggcg gggtcgatgg catcgatccg atttgtggcc
    12541 cctatcacca cgacctgtcc ccgctcttca agcccgtcca tcatcgttag gagctgggca
    12601 acaacgcgcc gctcaacctc tccggtcacc tcctcgcgcc ggggggcgat cgagtcgagt
    12661 tcgtcgataa agatgatcgc aggagcatgc tgccgtgcgt cctcaaagac ctcacggagc
    12721 ctctgctcgc tctcaccgta atacttcgag atcacctcgg ggcctgcaat cgagatgaag
    12781 tgtgctccgc tctcgcttgc aacagccttt gcaatgaggg tctttcccgt cccgggcgga
    12841 ccgtagagga ggacgccttt tggaggctcg ataccaagct tgcgaaagat ctccggatgg
    12901 cgcataggga gctcaatcgt ctcgcgaacg cgctgcaact catctttcag gccgccgata
    12961 tcctcgtagc tgatcctcct gacaccctca aaaccggccg caggcttctc tgagaactcg
    13021 atctttgtgt tcctggtgat tatcacggcg ttctccggct cgaccaccac cgccttgaaa
    13081 gcgacaattt gaggctgcat gaatggcagc cccacctgga taggcacgga atcatccctg
    13141 acgaccggga agtcgatcag cctgttgacg acgctgccgt agttgacggg gaggtgcttg
    13201 gggaggtctt cgggaggagc aagcacaacc cgtttagcct cgatctcctc atcgagtgtt
    13261 cgcaccctga cacggtcgcc tatgctcgcg ccggcgttta aacgggtgaa gttatcgatc
    13321 ctgactttgc cctgatgcca gtcgttcacc atggcgcgcc agaccttcgc tacggtgcgc
    13381 cgttttcctt cgatagcgac aaggtctccg gggttgagcc ggaggtgcag catggtatcg
    13441 gggtcaagcc gtgccttgcc tgccccctga tcctccgggt aagcgctatc taccttcaag
    13501 tatatctcgg gcattacctc tagttcttat atttcgggaa tttataggta ctggagtcgc
    13561 atgaacgtag ttctcttcga tccgttcaac ggagccgcag gcgatatggt aatcggatcc
    13621 ctcctcgacc tcggggccga tgaagaggct gtccgggcag ccatgcgctc cgtcgtcggg
    13681 gagccgacaa tcgaacgggt agaccgctgt ggtatccgtg cggtgcaggt gatgacgcat
    13741 gccaccgcgg agcaccgcac cctggatgag gtgctcgacc gcctggcagg gagcgacgcc
    13801 ccgaaagagg cgctggagat ggcacgaaaa gtctttctcc ggatacaccg ggcggaggag
    13861 agcatccacg gggcgggagc gcatttccac gaggtgggtg cagacgatgc catagccgac
    13921 gtggtggggg cgtgcaccgc tctacacagc ctggccccgg atggcgtcgc tgtcctgccc
    13981 ctggcgctcg gccgcggttt ttcggtcgga gctcacggta tcttccccat ccccgccccg
    14041 gctacggtgg cgatccttgc cgaatctcat ctgcagatgg ttcccggggg cgaagaacgg
    14101 gaactctgca ccccaacggg agccgcgctc ctctccgagt tctcgacgat ccggccggac
    14161 gaactgggcc cggcgagtat ccgggcggtc ggctatggcg cgggcagcag aaacccggcg
    14221 gaaagaccta acgtcctccg ggcgatgctc cttgaaacgg aggaagataa cagagggtcc
    14281 gaccggatcg atatactgga gacaaacgtg gacgacgtga cgggggagat gctcacctac
    14341 accctgggcc gcctgatgga ggagggggca cgggatgcaa ccgccatacc cgtgttgatg
    14401 aagaaagggc gcagcggcca taccgtcagg gtgatctccc cgccggcggc cacaggccat
    14461 ctcacggcgg tgatggcacg ggaactcggg acactcggga tacgctgcct cccgatggtt
    14521 caccgtgcag tcgctgaacg gacgatcgag agggttaagg tgaggtttgg ggggcaggag
    14581 tggtggatcg atgtgaagtg cggatggtcg gaaggcagga tcacgtccat aaaggcggag
    14641 tatgagcagg tggcacggtg cgcacgggag acggggcttc ctttcaggga gattgccgca
    14701 agggcggagg caaccgcccg ccgcctcttc atcgagcagg gtgtgctcga cccatgaaaa
    14761 ctgagcgggt cagcaccggg agccaggccc ttgacggcct cctcggcggc gggctggagc
    14821 gtggcgccat cacccagatc tacggcgaac cggcaagcgg gaagagcgtc ttctgcctga
    14881 tggcgacggt ggccaccctt aaggcaggga actcggtcgt ctacatcgac accgaggggt
    14941 tctcggtgga gcggtttgcg cagatcgctg gcggggatgc aggtgaactc gcggaacggc
    15001 tgtacctctt tgaaccgctt gatttcaccc agcagggagc gatgatcgcc gataccgagg
    15061 cgctgctaaa gaaggccggg aaggccccgg tggacctggt ggtgatggac tcggcgaccg
    15121 ccctctaccg gatggagctt gaccttggac gggaggcgtt gcggaaactc tcgcaccaca
    15181 tgataaaact cctcgggctt gcaaggaagt acgagatccc cgtgctgatc acaaaccaga
    15241 tctacatgga cgtggaacgc gaccgtgtgg ccggtcttgg ggggacggcg cttgaacacc
    15301 tctcgaaggc catagttcgg cttgaaagga gagacagtgt ccggcgggcg gttcttctga
    15361 aacaccggtc ccgaccggaa ggactctcgt tcgattttgc tatcaccggc gagggaatca
    15421 gaacgttata acgcccgaaa aaactgtctc cgccgggcct tccattgtaa cagtctcacc
    15481 aaaccggatc acgagcgggc cgcctctcgt ctccacctgc accgtatccc cgacaaggcc
    15541 aagacgacgg gcaaccgccg ctgaggcggt ggcgccggtg ccgcagctcg cggtctcgcc
    15601 ctcaacgccg cgctcaaaag tcctgatccg gatcttatcg aacgcgactt tctcggcaaa
    15661 gttgacgttt gcaccctccg ggaatgtggg gtggtgccgg attggcgggg cgaagatctc
    15721 gatctctaca gaatcgatat cgtcgacaaa gataacagcg tgcggaacac cgatgtttgc
    15781 ggcataaacg gtaaacccgc agatctcctc acggtaatca cctttcccga cagcggggat
    15841 tgcgctgcgc tcaaaagccg ggtctggcat ggtgatagtc gcgctgaact catcatccgc
    15901 ataacccatc ctcacagggg caatcccggc cccggtctct actgtgcagg gctctctgac
    15961 ataaccggcg tcgtaggcgt attttgcgag acagcggatg ccgtttccgc acatcgcggc
    16021 ctcgctcttg tcgggctgaa agagtctcat ccggatatcc gctttatcag acggctggag
    16081 gtagaggacg ccgtcagcac cgatgccaaa ccggcggtcg cagtagagcg ctgcaaaagt
    16141 tcctttcatc tcttcgggga tgatctcctg atcgaattca tcgatcagga tgaagtcgtt
    16201 gccgtttcca tgcagtttgg tgaacgtgat ctccatggtg taccctcttc tgtaatcatt
    16261 tggaacagcg ggtgataaac cgtgttgcct gctggaatgc aacactcgga accacaacaa
    16321 agagaggcca gggccgagat tcgaacccgg gtcgagggat ccacagtccc ttaggataac
    16381 cagctacccc accctggcaa agtactcata atagttggaa gtcgggtttg attaaggtat
    16441 ccctccccct gtacacgtct tccgcgcctc tcgaacggta acttcttcct ctgtccactc
    16501 accaccacaa gttaataggg tccatgctct ataacatacc agtatcaggc atctgctgac
    16561 gcccaactgt cagcagaacc aatccagacc ctggcgtggt tctggagagt tcgaggtcaa
    16621 tatcacgggc ttttcgggtt aatttgtcca cacaatatac tgcaggtgat tatcatggct
    16681 aaccaatcag cgatcagaac gcccatcgtc tgcgtgatgg ggcatgtgga tcacggcaaa
    16741 acgtccctcc ttgacaggat ccggggctca tccgtggtat ccaccgaaga aggtgcaatt
    16801 acacagcata tcggcgccac tctcgttcca atcgatgcaa taatacgcat gagcggggcg
    16861 ctgagcaggg tcaacatcaa cgtccccggc ctcctcttca tagatacgcc ggggcaccat
    16921 gccttcacca cgctgcgtgc gcgcggtgga gcgctcgccg atatggcgat cgtggtcgtg
    16981 gacatcaacg aggggtttcg tccccagacg atcgaggcgc tccagattct tcgcaactac
    17041 aagacgcctt ttgtgatcgc ggcaaacaag atcgaccgca tacacggctg gcgcgtcaat
    17101 gaggggcagc catttttaaa gacgtttgca caacagaacg agcgcgtcca gggggtgctc
    17161 gagacacggg tctatgaact tgtcggcaaa ctctccgacc tcggcttcaa ctccgagcgt
    17221 ttcgaccggg tctcagactt cgcgcggaac atctgcatcg tgcccacaag tgcactcacg
    17281 ggcgagggta tcccggacat cctgatgatg ctgatagggc ttgcccagcg ctacatgacc
    17341 gggagcctca gggtcaccgc cgatggcccc ggcgccggca ctgtgcttga ggttaaggaa
    17401 gagcggggtc tcgggatgac actcgatgtt atcctctatg acggcacact cagggtcgga
    17461 gacgatatcg tcgtcgcagg taacgaccag atcatcgaga caaaagtgcg ttccctctta
    17521 aagcccaggc cgatgagcga gatcctggtc gaagagcggt tcgaacgggt cagatctgtc
    17581 accgcagcgg caggtatcaa ggtcgccgca ccgaaactcg ccggtgtcat agcaggttcc
    17641 ccactccgtg ccgtccgggg cgggaaccgg gacgaggtga tcgaagaggt ccgccacgag
    17701 gtccaggata tccaggtgaa actctcggat acaggtgtca tcatacgggc agacaccatc
    17761 ggggcccttg aagctctatc aaaagaactc gatagccacc agatcccggt tatgcgggca
    17821 gtcgttggcc cggtcacccg ccatgacgtc attgaagccg ggactatcag ggatccactc
    17881 tacagcgcga tcatagcctt caacaccccg gtcctgccgg acgcgatcga tgccctggca
    17941 gacacgtcga tgtcccacgt cagcatattc gagggcgggg tcatctacca gctgatcgac
    18001 gactacatcg agtggcggga ggagaagaaa caggaactcg agcggcagag gtttgagaag
    18061 gtgatcatgc ctgccaggat ccggatcctt ccaaattgca tcttccggca gagcaacccg
    18121 gcggtggtgg gcgtccgtgt cctcggcggg aaactccaga gcggggttga ccttgtcctg
    18181 ccagagggga agaaggtggg ccggatcaaa cagatccagg agaggaacga gaccatccag
    18241 gaagctgagg cgggcaaaga ggtggcgatc tcgatcgatg ggccaacggt cggccgccag
    18301 atcaatgtcg atgacgaact ctacgttgat atacctgaac ggcatgtgaa ggtgatcgag
    18361 cgcgagatgc tggataacct gcgccctgac ctccgcgaga ccctggagga gttcaccgct
    18421 atcaagcgcc gggaaaaccc tttctggggg aagtgaaacc acttcatgag ttccgagccc
    18481 gcacagaggt agtcttttaa taccatattc tcaaagtgta taggtagttt tgaaggagtc
    18541 gtcaagatgg cagagttcaa aatagttcta tccgaccggg agaccgggcg ttcatataag
    18601 gtcgatgcaa ccggtcccgc cgcaggggca ctcataggca ggcgcatagg tgaggagatc
    18661 gagggtggtg ttctcggttt tgcgggctac aggatccgga tcaccggcgc ctccgatcga
    18721 acaggtattc ccgcgcgccg cgaccttcca gggccggcac gaagaaggct cctcctatcc
    18781 aggggtgtcg ggttccaccc cgtgagggag ggtgagcgcc ggaggaaatc agtgcgcggc
    18841 aacgagatca gtgctgatat cgtccaggtc aacgccgttg tgacacagcg cggttcaaag
    18901 cccctggaag aatacttcaa ccgtccggaa gcggcagccg aataaacaaa atccctattt
    18961 tctggtctac cggcaagtat ccgggctctt ctgagaatat cgagcagcac ggcagggcca
    19021 cgccccatgc cttatcgcag cataacatca tggcggtgtt atccatattc ctgcgatggt
    19081 ggcagggagt ggccatccag gcgatatcgc cttcagcgac gcccccgctg cgggactctc
    19141 ctgagagcac cggccatgat ccccatgaac tcttcgggac ggtttaagaa cgcaaccgaa
    19201 cggggctgcc cgatgagatc ataaagccgg gtttctctcc tgatcccgag ctccggaacc
    19261 tcaaccggat cgcagcagcg caccggcgga gcgtctggat agcgagggtt cttcaccagc
    19321 gccccccctt ccatctcgta gtaggctgca ccacgccttt caatgtaggg gccgtaatcg
    19381 ctggaaaaat ccgaggagac caggtttgcc ataacgagtt cctcccctcc ggggtttatg
    19441 gtcacgtggc cgtagcctgg cgggatcatg accttctcgc cgacggaggc ctcgatcatc
    19501 acaacgtcat cggcagggta tgtctggagg aggtagtgcc ccctgccttt gagaacctcg
    19561 tagatctcag gataatgctc ccctgcaggg ttgcgggggt ggtagtgccc tttcgtcttg
    19621 acgtactccc cgcagataac gcgagccggg atgacggtga tgtcgtagcg gatatggtgc
    19681 tgccggagcc actcccggtc actctcactt ctggcgagat cccgatacat aaaatagagg
    19741 ggttgatcag aggtgcagcc gggatctgca agcacatccc gcatatcctc gatcgtcctt
    19801 atctgcggct ccggtattgg tccgtcccag cagccgttca tcaatgtttc ttacggtaca
    19861 cgaatatata ataccctgcc ccgatgacaa cagccagaac gacgatgatg aagatgggat
    19921 ttgtgaagaa acctccctgt ctgcgctcga gcgcaacctg caccttcatg gtgtcggata
    19981 tcttgctgtt gtcgagtgca tcgcggtaac ggatctcaga atcgatccca tactccttct
    20041 cggtggcatc ggtatcgacg ttcacctcaa accgtgccgt tgcagtctcg ccgggagcga
    20101 ggtcgccgag gtaagcggtg tcatcgctgc tcgtgaatgg gtcgacagca ctgatacggg
    20161 cctgagcgct gtagaccgga gcagccccga cgttcttata cacgacctcg aggacgctct
    20221 tctgtccaag gtagagggtt gtgggtggtg agaccacctc aaagtcgatc ttgccgccga
    20281 ccggaagccc taccgtcacc acctttgatg tcaccgtctc accttcgtag ttctcatagg
    20341 ttacagcgac atccagggga taggactgct cttcggcggt gtcggcaacc gagaccttga
    20401 acttcacgtc taccgtttct ccaggttcaa gcatcccgat gtaggcgctg ccatcggtgg
    20461 ggatgagggg gcttgctccg cttctcgcga tctttacgat agccttctcc ccagtatcat
    20521 gcccgacgtt ctttatggtc atgtagacgt agccttcggt gccgacgttc agcgattcag
    20581 tgcgcacgtc aagaacctca accctgaggt caggggtgac acgaacctct agtggcaggg
    20641 tcacggtctt cttgatgtag ttgtagcgga gtacgtcggt gtgatcatcc accgtctcaa
    20701 tgtaggtgta ggtcacatcg agcggcagcg tgtatgtgcc gggttttgcc gagtccgcca
    20761 ccttcacgtt gaatgtggcg gtcgcggatg acccgcccct gatgtcgctc acaaactgcg
    20821 ggtctgtctt gacggtgaac ggcgcatctc cactcttcag cgccacggtg gccagttttg
    20881 ctgtgttcgg ctggtcatct ggaggcatcg acgggacggc gatcttgacg gtattcaggc
    20941 cgctgttgga tatgatgacc gtgaccgggg tctcgtcccc gggtgcaaac tcgtttgtgc
    21001 cggcgatcga tgcagagaga tcggggctcc cgtagaggta cttgacgccc tgcgccgagc
    21061 cgggcgcaac ggccaggagt gccagcagga gcaccaggat gggtatgtat tgcaaacgca
    21121 tctcttcacc ctgttgttat gttaacaaca acatttattg ccatactgta tatatacatg
    21181 atggaatgac ggcttcccgg gaagttcctg ccaccctgat cgagtcgctg aaatctctgg
    21241 gtcttacgaa gtacgaagcc ctggtatata taggtcttct aagggttgcg gacgcaacgg
    21301 cgacggaggt tcacgagatc tcaggtgtgc cgcgcgcatc cgtctacccg gttctcgacc
    21361 acctcgtcca gaaagaactg gtcattgtct cccacacgat gccaaagcgt ttcgacgctg
    21421 tcccccctgc acagggtata gagaacctta tgcgcaggat caggggtgat gcggagagcg
    21481 cgcgggaggc ccttgaggtc ttctaccggg aaaaagaact gatagaccgg ggtgcccgcg
    21541 agctgatctg gacaatctac ggcgaggaga atatcaagac ccggcttgcg gatctcttcc
    21601 gcagcgcgga aaaaagcgtt gaggtgcttg cgacccggga tctcctgcac gagaccgtcc
    21661 tccccctgct tgaatccctt ccagacgcgg tggaggtgga gatcgtcacc gcctggcagg
    21721 atgaggggaa cctgccgtcc cgcttcaggc tccgtctcct gcctctggca agttctccgg
    21781 ggaaactgct gccctgcgag aggtcaggcg tcttcctggt ggatgacgcc cgcgcgctgg
    21841 tctgggtggg agaggttgac agccagccgt ctgctctcta ctcggagtcg ggggggcttc
    21901 tcctcttcgt gcagcggcat atcaccacag tcaaggaatg ggcaagaaca acactcaata
    21961 atcgtcaagg tagcccatct tctgtatatc gatcaatccc ttgagcgctc gggtcaccac
    22021 aggctttcct gcctccctga gcgccgccat ggtgttcgtg cccccgacga atgccacacc
    22081 caggtactgc ggactcaccg ggacgccaag cagtggaaga tttggagcac cgacgtccag
    22141 gatcccggag aaaccgtatc cggaaagttc gtcaagagcc gtctcaagga ggtgctcggc
    22201 ctccatatgg cattcccgga tgtttgcgag tatgtttccc gtcccatgcc gcattacacc
    22261 caggatcgac gttatctcct gggatataag gacctcgagg gggtcgaggg tggtgtcgcg
    22321 gtagaggatc atacacgtga acctccgcgg gatcctgcct tcgatctcga ctatcccgcc
    22381 accgatgggg ttgagcggaa tcccgcgctg gatgaggagg gcatcgagag tgatgctgca
    22441 gatcgtgcat atggcgtttc gaccttccgc cacagtgtag ccatcaacct gatctcccgg
    22501 cccgctgatg agcagcctgt cgctgatggt gataccctgc cggtgcgcct ccttcatgat
    22561 ggagattgcg aactcaaggt cgcggctctc gataaccgag aggttgtaga tcacctttcc
    22621 cgcattttcc tccgggcgat aggtcacctt gagcgcgtag tcctcgatac ggtggttggt
    22681 gaacttcagg ggggcatgca ttaccagact tctgccgcag caggggaaaa gggttgcctt
    22741 caggatcagg tgagcctcat tcgggctgca ggg
//
LOCUS       DAOSZX010000013        22210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1922_length_22210_cov_19.798104, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000013 DAOSZX010000000
VERSION     DAOSZX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22210
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_22210_cov_19.798104"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1721)
                     /gene="purH"
                     /locus_tag="PK196_02200"
     CDS             complement(234..1721)
                     /gene="purH"
                     /locus_tag="PK196_02200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HON40269.1"
                     /translation="MKWALLSVWDKTGIVDLAKVLIEHDYRILSSGGTGAALDKAGIP
                     FTDVSTYTGSPEMMDGRVKTLHPKIHGGILGRRGIDDALMQEHGIEPIDLVVVNLYPF
                     EAMSGKGLPIDKVIEYIDIGGPAMVRAAAKNHRYVAVVVDPADYDKVAEAIRRGGFTA
                     EERLNLAAKAFARTAAYDGAISNYFTGIGRPFPTILTLQHRGGRTLRYGENPHQAAAV
                     YGEAGIAGEEPLQGRQMSYNNYLDVHAAVGLLREFNDGCAAVIVKHNNPCGVAIGNGL
                     LETYIAAREVDPVSAYGSIVAVNKEVTEDVAREITKTFVEVLIAPSYTTGALEIMKAK
                     ENMRVLRLPHPVIHDEIRSIDGGILVQRTEPYRENWRVVSEREPTAHEMLAMELAWKV
                     CKHTKSNAIVFADEKAVIGIGAGQMNRVESAEIAVRKARKSLAGSAVASDAFLPFPDT
                     LEVAAAAGATALVQPGGSIRDQEVIDAANRLNVAMVFTGVRHFRH"
     gene            1962..2492
                     /locus_tag="PK196_02205"
     CDS             1962..2492
                     /locus_tag="PK196_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HON40270.1"
                     /translation="MKEDTSKPPGEQQALAEKADAASREREEVQTSYDELKSAYDDLN
                     NRYLRLAADFENYRKRMARELENLARLAIEDLAVELLEVVDNFERAEKAEAGGLGEGM
                     QQIQKLLIDILERHGIKPIECLNRPFDPECQEAIACVPSEAEEGSVIEEVVRGYCMQD
                     KVIRCAKVVVSKGKEE"
     gene            2497..4302
                     /gene="dnaK"
                     /locus_tag="PK196_02210"
     CDS             2497..4302
                     /gene="dnaK"
                     /locus_tag="PK196_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HON40271.1"
                     /translation="MVSEKVIGIDLGTTNSCMAIMEGGRPTVIPNAEGGRTTPSVVAF
                     TKDGERLVGSAAKRQAITNPERTIQSIKRKMGTSEKVTIGDKTYSPQEISAMILQKLK
                     VDAEQYLGEKITKAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTASALAYGID
                     REEEATVLVYDLGGGTFDVSILQLGDGVFEVKATAGINHLGGDDFDKRIVDYLVDEFR
                     KKEGIDLRKDRVAMQRLRDAAENAKIELSSVQKTNINLPYITTTESGPKFLDIDLTRA
                     KFESLIADLVDSTIGPVKQALADAKLSPEDIDHVLLVGGSTRVPLVQETVRKILKKEP
                     DKGINPDECVALGAAIQAGVLTGETKDVLLLDVTPLSLGIETLGGIATKLIERNTTIP
                     TRKSQIFSTAADGQTSVEIHVVQGERALAKDNFTLGRFQLTGIPPAPRGVPQIEVTFD
                     IDANGIVHVSAKDLGTGNEQSITIKPQDSRPSEAEIQRMMDEARRFEEEDRKKREEIE
                     LRNAADTAIFTAERAIKDAKDTIETSDRERIESAIADLKKALEGDDTEAIRQKMDALT
                     EAVYAFTTKVYQRAQAGTAAGKDDNVVDADYEVKE"
     gene            4308..5471
                     /gene="dnaJ"
                     /locus_tag="PK196_02215"
     CDS             4308..5471
                     /gene="dnaJ"
                     /locus_tag="PK196_02215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HON40272.1"
                     /translation="MGPESYYDTLGVSRDADDKEIKKAYRNLARKYHPDVCKEPGAEE
                     RFKKINEAYSVLSDPEKRAQYDRMGHEAYSSASRGSYSGGGYSSGFSADFSGFGDIFE
                     TFFGGGFAGGGRQHTGPRPGADILVKLRITLDEAVFGTEKEVEVEHVEPCLECDGTGS
                     ETKKTVTCPTCGGTGQMRQMSQSLFGNFVRMSTCSTCNGQGRVPETRCRACNGTGHRR
                     ARRTVKVRIPPGVDTGMRLRMEGYGDFGDYGAPPGDLYVEITVAPNRAFTRRGDDLET
                     TVDITPAQAALGSEVEVQTIDGRTVSLSVPAGVQHDTRLRIRGEGVRWQTKPGDLLVR
                     VRIVIPERLTDEERELYERLLEVEGHKPSSVKGAKKGKGFFHDVVDRMKETVK"
     gene            5571..6524
                     /locus_tag="PK196_02220"
     CDS             5571..6524
                     /locus_tag="PK196_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HON40273.1"
                     /translation="MKLLFELSGEHPDLPVAELECVGRVLSRRTQVAVAECPDPEATR
                     RLSLTHVVMEYLGECEPTAAAFGGLLRDLGITTTRPFAGRVKTVQGARMDISSLEAER
                     MIGSLIAGPVSLRNPIEEYRAIVSDDRCYFGRVIFRIDRGAFEARNPTRRPFFHPGVM
                     MPRIARALVNISLVAPGERLFDPFCGTGGILLEAREVGARILGSDFDPEMIEGCRRNL
                     PGPDVMVADAAAVPIRDSAIDAVVTDLPYGQSVRVRAESMDQLYDGSLAEIRRILRPG
                     RRAVVVTNRDITSIAACYFTILQEHEQRVHKSLTRRILVLS"
     gene            6570..6938
                     /locus_tag="PK196_02225"
     CDS             6570..6938
                     /locus_tag="PK196_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40274.1"
                     /translation="MEINFKLYGLYLVRWQLSTPILAGVLALLGSLGALAATIIANLV
                     GGLIFFWVDRFIFTSETLAAQWEVRDDIRCVDCGRVARGYRLVRAKNYDRTSDPAPEF
                     RCEACSKKKSDELKKRGIDH"
     gene            complement(7110..7814)
                     /gene="pyrH"
                     /locus_tag="PK196_02230"
     CDS             complement(7110..7814)
                     /gene="pyrH"
                     /locus_tag="PK196_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HON40275.1"
                     /translation="MKKIVISLGGSILVPSLESNNIARYVSVLKKISGICRLFIVVGG
                     GGEARRYIGVARGLGAGEAAADELGIMVTRLNARLLIAGLGEAAYPRVAENYTEALEF
                     ALSGKIVVMGGITPAQTTDAVSAVLAESVGADLLINATSIDGIYSADPKKDASAVRHE
                     HLTPRELLEILTANRMNAGANTVLDIVAGKVIERSGIPLLVIDGREPDNLYRAIAEGA
                     FVGTIVSKEDAEPRPA"
     gene            7855..8022
                     /locus_tag="PK196_02235"
     CDS             7855..8022
                     /locus_tag="PK196_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40276.1"
                     /translation="MKMLHCPVCNSTDVWPVTGGYIGFLYRCKRCGYRGALVVEYDRE
                     AGEVDKRHLPQ"
     gene            8053..9066
                     /gene="amrS"
                     /locus_tag="PK196_02240"
     CDS             8053..9066
                     /gene="amrS"
                     /locus_tag="PK196_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HON40277.1"
                     /translation="MHEARLYQKLEDNTVRCSLCAHRCTIKDGKHGICGVRINRGGTL
                     YAATFGKVIAEAVDPIEKKPLYHFLPGSLSYSLGSVGCNFHCVHCQNWQISQQTLDMG
                     GFRDISPEMGVERAIASGSASIAWTYNEPTIWHEYPLEMGALARQKGLGTVYVTNGYI
                     TEEGLRDLAGMLNAFRVDIKSFSDAFYRKVCGGRLQPVLDAALLAKELGMHIETVTLV
                     IPGQNDSMEEMEGLIRWVVENLGPDTPMHFTRFHPEYRMLDVWPTPVRTLEKVYERAK
                     ELGINYPYLGNVGGHPYESTYCPSCGNLIIERAGYAIRIRGLEGHSCTRCGGHIEYVS
                     EIR"
     gene            9047..9538
                     /locus_tag="PK196_02245"
     CDS             9047..9538
                     /locus_tag="PK196_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HON40278.1"
                     /translation="MSRRSGERRFLTDRMLGTLTRYLRFMGYDTLSANGLSPGSTRED
                     SLLLEIAGQEDRVLLTRDRELARRGGEQAVYIASEDILSQIRQLADVGLIEPVVRMSR
                     CSLCNTRLRPATQREIRETPYAPPSPPEREFWWCPACRKLYWMGSHGARIERRLKEVR
                     PGS"
     gene            complement(9757..10365)
                     /gene="hisH"
                     /locus_tag="PK196_02250"
     CDS             complement(9757..10365)
                     /gene="hisH"
                     /locus_tag="PK196_02250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HON40279.1"
                     /translation="MKKKIVIIDYGLGNLRSVFRGLERAGAAPRTTGDAEEITAADGI
                     VLPGVGAFREGMEMLGDLQATVVNAARETPLLGICLGMQMLMDSSEEHGLHRGLGLIP
                     GEVRRFVPTGGEKVPHMGWNTIRIQAGAPLFDGLHEEEYVYFVHSYYAAAAPESTIAS
                     TTYIHPFASAVRCGQTYGLQFHPEKSGAVGLKILENFIEQIC"
     gene            complement(10340..11749)
                     /locus_tag="PK196_02255"
     CDS             complement(10340..11749)
                     /locus_tag="PK196_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HON40280.1"
                     /translation="MPRIYLGEILLHWCDTCHVPVLSGVCACGSPTRPVAVTPPGDAR
                     PAFPDDIERINRIFSDHFGAPLIPEGHIALLNKVPAADRMDEIVLGGAVVGAVRYLPG
                     ERRWEPLPRRAAAAYMRPTRRRVVVDDGAVPSIRDEGASVLAPGIISIDPAVAAGDEV
                     FILSANGECIGVGRAKVDAATAGAMERGVVVRTRKNLDAAPLPGEAAWEDTVRANEPV
                     LADYEAASIRFVREAAEQNPHTPTISYSGGKDSLATLLIVLKAIGAVPILFSDTGLEF
                     PETYENVDEVARRYGLEVFSACDKEAFWETFEENGPPAVDNRWCCRVCKLHPVGRLIE
                     ENWGECLSFIGQRKYESVRRMRSRRVWRNPHVPQQVSAAPIQQWTAMHVWLYIFREKA
                     PYNRLYERGLDRIGCFMCPSSDLATFAIIGETHPELMKMWHEKLARWQEKQGLDPDWI
                     ESGLWRRQGSSDEEEDSYN"
     gene            12058..12378
                     /locus_tag="PK196_02260"
     CDS             12058..12378
                     /locus_tag="PK196_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40281.1"
                     /translation="MHEIGSGGMASMRFARKPLRKIEILGLIIFILMAGVMLAVGALL
                     FCDWLDAPGSAPAGSPADPGPVLSFRSIAGAIPLIPLVVGVEVAGALSLTVLYLVSAL
                     RRRK"
     gene            12464..12619
                     /locus_tag="PK196_02265"
     CDS             12464..12619
                     /locus_tag="PK196_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40282.1"
                     /translation="MGYGKFYIPTGIHHVYPSLGRRCVMAKDKRSQDKKAEEKKVEKG
                     QEKAKKN"
     gene            13134..13206
                     /locus_tag="PK196_02270"
     tRNA            13134..13206
                     /locus_tag="PK196_02270"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13167..13169,aa:Ile,seq:gat)
     gene            complement(13312..14610)
                     /locus_tag="PK196_02275"
     CDS             complement(13312..14610)
                     /locus_tag="PK196_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HON40283.1"
                     /translation="MEFSCGIDERPRPGLLLVYGLQWLVVSVPVILIAGRVVAGLEAG
                     GAAIPYLQRLFLLVAVLLVVQVYMGHRLPIVLGPATVLLVGILASQDAGSAAINASIL
                     IGGLLLSAIALTGFVGHLKRLFTPRVIVVVLVLIAFTLAPVILGLVTEGGDGVTATAA
                     FLFAAGFALALFAAHGILGGFWRSTLAVWGLVFGTAVYIALFGGAAALPADTALFALP
                     GDLAAPLAIPDPGVLAAFLICYLALATNDLGSIQSVGSLLAAEGMDERVNRGVGVTGL
                     GNVIAGLMGVIGPVNFSLSPGVIAATGCASRFALVPAAVGLALIGLSPRAIGCMECIP
                     GPVIGVVLAYVMATQIAAGLVFGQESGALETFDGGLIVGIPLLLGTLVAFLPAEVVAG
                     LPTVLRPVLANGFVVGIVTVLGLEHVLYRRRSAKPPNETG"
     gene            14762..15703
                     /locus_tag="PK196_02280"
     CDS             14762..15703
                     /locus_tag="PK196_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40284.1"
                     /translation="MAAMNAVDVRGLSRSFDGREILRDVTFSVAHGEIFGYLGPNGAG
                     KTTTIRILLGLLTPGAGECRVLGRDLAIDDAARAKVGVLFENNGLADRLSAADNLAYY
                     AGLYDLDDPRDRIDELLALVDLEDRRNDPVGTFSTGMKRRLGIARAILHRPEVVFLDE
                     PSSGLDPGAQRMVRDLIVDLSRREEMTVFLNSHHLDEVQRICSTVAILAGGRIRAFDS
                     VKNLTAASGSPEMTVVLADPDDRARARETAMALPYVSGCEEDGEVLRCTLTDPGVIPD
                     LIAALAAAGFRLLEVRRSSRSLEEIYLENVGQSEGGS"
     gene            15700..16401
                     /locus_tag="PK196_02285"
     CDS             15700..16401
                     /locus_tag="PK196_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40285.1"
                     /translation="MSTFTVIAGREIHIALRNRGIIITALIFAVWFPALSAVGMVATF
                     GGGAEAVSGAVAAVTLPVAILMGYIFCSDAFLREKRDGSIETLLCAPVSLRHLWAGKT
                     CGVAIPAYLTTLLSAAVTLAMASAMLEAPPSLDLLLIPHMLVVVPVWILTAAGFVGAA
                     QLALGMRENQILGFLFIFGFIFLIGGLQGIISGGAEISAAAEAALAAAGAALLGLARF
                     IAGKVSKERIVTTIP"
     gene            complement(16423..17700)
                     /gene="serS"
                     /locus_tag="PK196_02290"
     CDS             complement(16423..17700)
                     /gene="serS"
                     /locus_tag="PK196_02290"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HON40286.1"
                     /translation="MLDLKFVREHPEIVRADLMKRGDTDKLPWVDEVLEMDRRVRELT
                     VEIGNLRNRRNVVSREISRARKAGEDISPLLSEAAGLPERIREAEAERGRLSEAVRYR
                     LMRLPNILHESVPVGSDETGNVEIRRWGEPKVPEFDLVNHGTLAVEHDWADFERAAKI
                     AGAGFYFLKGRLALLDMALQRFAMDLLIRRGYTPIIPPYMMNRASYEGVTDLADFENV
                     MYKIDGEDEYLIATSEHPMAAMYSGEIFEEKDLPLRLAGISPCFRREIGAHGIDTKGL
                     FRVHQFHKVEQFVYSTPEQSWDLHEELIANAEEVFQRLGLPYRVVLICTGDIGTVASK
                     KYDLEVWMPREERYREAVSCSNCTAYQAVRLNIKVRDPVEFTRKRYLHTLNSTAIATT
                     RAIRAILENYQNPDGSVTVPEALRPYLYGEETL"
     gene            17811..18263
                     /locus_tag="PK196_02295"
     CDS             17811..18263
                     /locus_tag="PK196_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40287.1"
                     /translation="MDRNQKIVLVAGGLITLGLLFIDYFVALIALVCLLTLLMCLHIM
                     RETADFPLVTADLSEDARRIILTNTGTAVANNIHLALVPLNIEFDHPSLGPDERSSLE
                     LESMVSEAKAVVTYENAGGERFQRSYPLTALRAGHDPTEPMFPVFGKR"
     gene            complement(18271..18798)
                     /locus_tag="PK196_02300"
     CDS             complement(18271..18798)
                     /locus_tag="PK196_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40288.1"
                     /translation="MDPIIEEGYNRLLETLDTLEAEKEEAAAKVRENAATLLARMAAD
                     AAPAVKKVGLEMLRRARREASGQLYDQEFYEKRMILLGKGEPLPYRPDDTAKPVDVQI
                     CVLDEDGVFHELMYTNTEIRTDSYLSVLTPDEAFEIYGYEILFMLYRALYEYAEKEED
                     LMAALARTLEYIAIP"
     gene            complement(18803..19639)
                     /locus_tag="PK196_02305"
     CDS             complement(18803..19639)
                     /locus_tag="PK196_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HON40289.1"
                     /translation="MLEVLGFEFFRNALLAGVFASIACGIIGTYVVVRRMVSVSGGIS
                     HAAFGGVGLGYYLGIDPLLGATVFTVATALGMGVLEIRARQQMDTIIGAVWAAGMALG
                     ILFVYLTPGFAPDLFSYLFGNILLVPRGDILLMAVLVAIIVAIVGLFYLEFQAVTFDP
                     DYARVMNLPLERLSLLLLVLVALTVVMLIRVVGIILVIALLTIPAAISRLYTGRVWSM
                     MLLATGLGALFTLIGIGLSYVLNVPSGATIILVSTAAYAAALGIRGGRSVRLWRDTQN
                     GE"
     gene            complement(19632..20396)
                     /locus_tag="PK196_02310"
     CDS             complement(19632..20396)
                     /locus_tag="PK196_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40290.1"
                     /translation="MSEPLIEVRDVWVTMRGHTVLEAVNLSIYPDDFFAIIGPNGGGK
                     TTLLRVILGLLRPCRGEVRFRGGMSSLGYVPQFRTFDFDYPITVREMVISGRLGRISR
                     LPRRYGSLDRERADEALETMGISDLADRQIRDLSGGEQQRAIIARALVGEPNVLLLDE
                     PTVYVDTPTTLQFYEILDQLRERMAIVLVTHDIGVIPDRITRVACLNRRLYTHDSAEI
                     TPDMLEAAYHCPVDLIAHGVPHRVFPDHAGEGGEDA"
     gene            complement(20393..21274)
                     /locus_tag="PK196_02315"
     CDS             complement(20393..21274)
                     /locus_tag="PK196_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HON40291.1"
                     /translation="MMLDTTGIKPLSLVAVACLLLGLMTAMAGCTGADGDVEDDGRIA
                     VVVTIPPQQEFVERVGGDHVRVILLVPPGADPHTYEPAPAVLAAVAEADLYAMVGSGI
                     EFEIAWGEKIAALNPDMTVVNCSQGVEVIAADPHIWTSPRNAKVMVENIRDGLIEADP
                     ENAEDYRRNAAAYLDDLDALDAEISALIAGSGVRVVLVDHPSWAYLARDYGFEEVAIE
                     SEGKEPSPKRIEHLIRLAEEEGVRVVFASPEHSTRSAGVIAEAIGGSVVTVSPLKKDY
                     LDNMRQVAAAFAGSVSG"
     gene            complement(21312..21752)
                     /locus_tag="PK196_02320"
     CDS             complement(21312..21752)
                     /locus_tag="PK196_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HON40292.1"
                     /translation="MQEFTGQELSSKKADYLKYIYMRGDLVKTTEIAARFDVAPSTVT
                     KTLREIANAGYIEHTPYHGVRLTPPGVEYARFLVRRHRIVALVLSHYRLTPEEACSEA
                     EKIEQSFSKDLVDRICWSLGHPMMSVCGEIEHDHGCCSSGGRLV"
BASE COUNT         4484 a         6771 c         6616 g         4339 t
ORIGIN      
        1 cccatgcgtt ctttcgcaat gcgagcgtat cgttatagac ccaccggcat atctccagcg
       61 tctgctcaag cagagtcgat acccgatatg ccttacgcat tatagtctca tacgccaagg
      121 tgcgcaatat ggttatcgat ggacggcatt tatccccgca ctaaagtgtg gggccttctg
      181 cctgttttct tcgtaaaagg atgcaaataa cacactgacg ataaaaaagg tttttagtgc
      241 cggaagtggc gaaccccggt gaagaccatc gcgacgttga gcctgttcgc cgcgtcgatg
      301 acctcctggt cgcggatgga ccctccaggc tggacgagcg cggtcgcccc tgccgccgct
      361 gcaacttcca gcgtatcggg gaacgggagg aacgcatctg atgccaccgc cgatccagca
      421 agggattttc gggccttcct gaccgcgatc tccgctgact cgacccggtt catctgcccg
      481 gctccgatcc cgatgaccgc tttctcgtcg gcgaatacga tggcgttact cttcgtgtgt
      541 ttgcagacct tccaggcgag ttccattgcc agcatctcat gggccgttgg ttcccgctcg
      601 ctcacgaccc gccagttctc ccggtatggt tctgtgcgct ggaccaggat gccgccgtcg
      661 atgctccgga tctcatcatg gatgacaggg tggggaagcc ggagcacccg catattttcc
      721 ttggctttca taatctcaag cgcgccagtc gtgtaggatg gagcgatcag aacttcgacg
      781 aacgtctttg tgatctcgcg ggcaacatcc tccgtcacct ccttgttcac ggcgacgatc
      841 gaaccatagg ctgagactgg atcaacctcc cgggcggcga tataagtctc aaggagtccg
      901 ttcccgatag ccacaccaca ggggttgttg tgcttgacta tgacagcagc acacccatca
      961 ttaaactccc ggagcagccc gaccgctgca tgaacgtcaa gataattgtt gtatgacatc
     1021 tgcctgcctt ggagcggctc ttcgccggcg atacccgctt ccccgtagac cgctgccgcc
     1081 tgatggggat tttcgccgta acgcagcgtt ctacctcccc tgtgttggag agtgagaatc
     1141 gtcgggaatg gccggccgat accggtgaag tagtttgaga tcgctccgtc gtaggctgcg
     1201 gttcgggcga acgcctttgc agcgaggttc aggcgctctt cggctgtgaa accgcctctc
     1261 ctgatggcct ctgcaacttt gtcgtaatca gcgggatcca ccaccacggc aacgtatctg
     1321 tggttctttg ccgccgcccg caccatcgcc gggccgccga tatcgatgta ctcgatcacc
     1381 ttatcgatcg ggagcccctt cccgctcata gcctcgaagg gatagaggtt caccacgacg
     1441 aggtcgatag gctctatgcc gtgttcctgc atcagagcgt cgtcgatacc ccggcggccc
     1501 aggatcccgc cgtgaatctt tggatgaagg gtcttgaccc tgccatccat catctcggga
     1561 gaaccggtat aggtggatac atcggtgaac gggatccccg ccttatccag cgccgccccg
     1621 gttcctcccg aactcaggat acggtaatcg tgctcgatga gcacctttgc gagatcgacg
     1681 atgcctgtct tgtcccagac agacagcagt gcccatttca tggttacacg taagcggggt
     1741 ggagagatat atctggcgct agccgcaaaa ccggttggca aatatggcga tcccttcccg
     1801 gaagattttt atcctaccgg aagaatttct gtggatttgc gtctgatcgt aaattagaca
     1861 aatgagaggg gataaatgcc cacactgata gttaataata tatagaaccc cattacaata
     1921 aataataaac acatgatact ttttaaaagc aggttaggcg tatgaaggaa gatacatcca
     1981 aaccacctgg ggagcaacag gctcttgcag aaaaggcgga tgcggcatct cgagagcgtg
     2041 aagaggtgca aacgtcatat gatgaactga aatcggcata cgatgacctt aacaaccggt
     2101 atctccggct tgcagcagat tttgagaact accgcaaaag gatggcccgt gaacttgaga
     2161 acctggcgag gcttgccatc gaggatctcg cggtcgagtt gctcgaagtt gtggacaact
     2221 tcgagcgggc ggagaaagcc gaggctggcg gtcttggaga aggtatgcag cagatacaaa
     2281 aactccttat agacatcctg gaacggcacg gtatcaagcc tatcgagtgc cttaaccgac
     2341 cttttgaccc ggaatgccag gaggctatcg cctgcgtccc gtcggaggcg gaggagggca
     2401 gcgtgatcga ggaggtcgtt cgcggctact gcatgcagga taaggtcatc agatgcgcaa
     2461 aagttgtagt gtctaaagga aaggaggaat aaccacatgg tttcagagaa ggtcattggc
     2521 atcgatcttg gaacaaccaa ctcatgcatg gcgatcatgg aaggcgggcg gccgacggtc
     2581 atacccaacg cggaaggcgg caggaccacc ccctcggtcg tggcgttcac aaaagacggg
     2641 gagagactgg tcggcagcgc agcaaaacgg caggcgatca ctaacccgga acgcaccatc
     2701 cagtcgatca aacgcaagat gggcacaagc gagaaggtca ccatcgggga caaaacctac
     2761 agcccgcagg agatctccgc gatgatcctc cagaagttga aggtggatgc ggaacagtac
     2821 ctgggtgaaa agattacaaa agcggtcatc acggtgcccg cctacttcaa cgatgcccag
     2881 cggcaggcga ccaaagacgc agggaagatc gcaggcctcg aggtgctccg gatcatcaac
     2941 gaaccgaccg caagcgctct tgcttacggt atcgacaggg aggaggaggc aacggtgctc
     3001 gtctacgacc tgggaggcgg cacatttgat gtatccatcc tccagctcgg ggatggcgtc
     3061 tttgaggtca aggcaaccgc tggcataaac catctcggcg gcgacgactt tgacaaaagg
     3121 atagtcgact acctggtcga tgagttccgg aagaaggaag gaatcgatct ccggaaagac
     3181 cgggttgcca tgcagcgtct gcgtgatgcg gccgagaacg caaagatcga gttatcatcc
     3241 gtccagaaga ccaacatcaa ccttccctac ataaccacca cggagagcgg ccctaaattc
     3301 ctggacatcg atctcacccg agcgaagttt gagagtctca tcgccgacct ggtggactcg
     3361 accatcggac cggtgaaaca ggctctggcc gatgccaaac tgagtccgga agatatcgat
     3421 cacgttctcc ttgtaggggg gtcaaccagg gttccgctgg tccaggagac tgtgagaaag
     3481 atcctcaaga aggagcctga caaaggtatc aatccggacg agtgtgttgc actgggtgct
     3541 gctatacagg caggcgtcct caccggggag actaaagatg tccttctgct ggatgtgacc
     3601 ccgctctcgc ttggcatcga gacgcttggc ggcatcgcga cgaaactgat cgagcgcaac
     3661 accaccatcc cgacgcggaa gagccagatc ttctcgaccg ccgccgatgg acagacatcg
     3721 gtcgagatcc acgttgtgca gggcgagcgg gcgctcgcga aggataactt tacacttggc
     3781 cggttccagc tgacagggat tccgcccgcg ccacgcggcg ttccgcagat cgaggtgacc
     3841 ttcgatatcg atgcaaacgg tattgtccat gtgtcggcaa aggatctcgg taccgggaac
     3901 gagcagtcga tcaccatcaa accgcaggac tcccgcccat ccgaagcgga gatccagcgg
     3961 atgatggacg aggccaggag gtttgaggag gaggaccgga agaagcgcga ggagattgag
     4021 ctgcgcaatg ccgccgatac cgcgatcttc actgcggagc gggcgatcaa ggatgcaaag
     4081 gacaccatcg agacctctga cagggagagg attgagagtg cgatcgccga tctcaagaag
     4141 gctctcgagg gtgacgacac cgaggcgatc cggcagaaga tggatgccct gaccgaagcg
     4201 gtatatgcat tcacgacaaa ggtgtaccag cgtgcgcagg cggggactgc cgcggggaag
     4261 gatgacaacg tcgtcgatgc tgattacgaa gtcaaagagt gagaggaatg ggtccggaaa
     4321 gctactatga taccctcggc gtttcacgcg acgctgacga taaggagatc aagaaagcct
     4381 accggaacct tgcacgaaag tatcacccag acgtctgtaa agagcctggg gccgaagagc
     4441 gtttcaagaa gatcaacgag gcttacagtg tcctctccga tcccgagaaa cgcgcccagt
     4501 acgaccgtat ggggcatgag gcctacagca gcgcttcccg cggttcatac tccggtggtg
     4561 gatattccag cgggttcagc gctgacttct ccgggttcgg ggatatattt gagaccttct
     4621 ttggcggtgg gtttgcaggc ggggggaggc agcatactgg cccgcggccg ggtgcggaca
     4681 tcctggtgaa gttgcggatc acgctcgatg aggccgtgtt cgggacggag aaggaggtcg
     4741 aagtagaaca tgtcgagccc tgcctcgagt gcgatggaac cgggagcgag acgaagaaga
     4801 ccgttacctg tccgacctgt ggcgggaccg ggcagatgcg gcagatgagc cagtctctct
     4861 tcgggaactt cgtacggatg agcacatgca gcacctgcaa cggccagggg agggttccgg
     4921 agacgcggtg cagggcatgc aacggcaccg gccaccggcg tgcccggcgg acggtgaagg
     4981 tccggatccc gccgggcgtg gatacgggca tgcgtctccg gatggagggc tacggcgact
     5041 ttggcgacta cggtgcgccg ccgggcgacc tttacgtgga gatcaccgtc gcgccgaaca
     5101 gggcgttcac ccgtcgtggc gacgacctgg agaccactgt cgatatcacc ccggcacagg
     5161 cggcgctggg ctctgaggtg gaggtgcaga cgatcgacgg gcgcacggtc agcctcagtg
     5221 tcccggccgg agtgcagcac gatacccggc taaggatacg cggtgagggt gtgcgctggc
     5281 agacaaagcc cggggatcta ctggtgcggg tgaggatcgt catccccgag cgcttgacgg
     5341 acgaggagcg ggagttgtat gagcgtctgc tcgaagtgga ggggcacaaa ccctcttctg
     5401 tgaagggcgc gaagaagggc aagggcttct tccacgatgt tgtcgatcgg atgaaagaga
     5461 ctgtgaagtg atggtctttc ctggctttgg tgtctggttt tttctgtttt tcgggctgat
     5521 gcggatccgc cgatcttgtt gttatacccg gcatgcccaa taccgtgagg atgaaactgc
     5581 tttttgaact ctccggtgag caccctgacc tgccggtcgc ggagctcgag tgcgtgggca
     5641 gggtgctctc ccggcggacg caggttgcgg ttgcggaatg ccctgacccg gaggctacca
     5701 gacggctttc cctgacgcac gtggttatgg agtatctcgg cgagtgcgaa cctacggctg
     5761 cagcgtttgg gggtctgctt cgggaccttg gcatcacgac cacacgaccg tttgccggca
     5821 gggtgaagac cgttcagggg gcgcgtatgg acatatctag ccttgaggca gagaggatga
     5881 tcgggagcct tattgccggc ccggtctcgc tccgcaatcc tatcgaagag taccgtgcca
     5941 tcgtctcaga tgatcgctgt tacttcggcc gcgtaatttt ccggatcgac cggggggcgt
     6001 ttgaggcccg gaaccccacg cgtcggccat tcttccaccc tggcgtgatg atgccccgga
     6061 tagccagggc gctcgtcaac atctctcttg tcgcgccagg tgaacggctc tttgacccct
     6121 tctgcggcac aggcgggatc ctccttgagg cgagggaggt aggtgccagg atccttggga
     6181 gtgattttga ccccgagatg atcgagggct gcaggcgcaa cctccctggc ccggatgtga
     6241 tggttgcaga tgccgcggca gtccccatcc gtgacagtgc tatcgacgcc gttgtgacag
     6301 atctgcccta cggccagtcg gtcagggtcc gcgcagagag catggatcag ttgtacgacg
     6361 ggtcgcttgc ggagatccgg cggatccttc ggccggggcg gcgcgcggtc gttgtgacga
     6421 accgtgacat aacgtccatc gccgcctgct atttcaccat cctgcaggag cacgagcagc
     6481 gggtgcacaa gagcctgact cgccggatcc tggtgctctc ctgaggtcgt ccccgcccgg
     6541 ttttatgact caggggttga atattatctg tggaaattaa ttttaaacta tacggcctgt
     6601 atctcgtccg gtggcaactc tcgacgccga tcctggccgg ggtgcttgcg ttgctcggca
     6661 gccttggggc gcttgccgcg acgataatcg ccaacctggt gggaggtctc atcttcttct
     6721 gggtcgatcg gtttatcttc acctccgaga ccctcgccgc acagtgggag gtgagagacg
     6781 atatcaggtg cgtggactgc ggcagggttg cccggggtta ccgcctcgtc agggcaaaga
     6841 actacgacag aaccagcgac ccggcgccag agtttcgatg tgaggcatgc tcgaagaaga
     6901 agtctgatga actcaagaaa cgtggtatcg atcattaaga ggggtttgaa cctggaaaga
     6961 gaacctctcc tttgcgctgc agactctctc tgcggcaatc gggttctttc tggcggcaag
     7021 gcagatcatc tccaggttcc ctgtctagtt gggtttagaa tcctgtttgc tgccaccgga
     7081 tcgctggagg ggagtttttt cctgaaaatt caagccggcc ggggttctgc atcctctttt
     7141 gagactatgg tgccgacgaa cgcgccctcc gcgatcgccc ggtagaggtt atccggttca
     7201 cggccgtcga tcaccagaag agggatcccg gagcgttcaa taactttccc ggccacgata
     7261 tcgagaacgg tgttggcgcc cgcgttcatt ctgtttgctg tgaggatctc caggagttct
     7321 cgcggagtga ggtgttcgtg cctcaccgca ctggcatctt tcttcgggtc ggcgctgtat
     7381 atcccgtcga tcgacgtggc gttgatgagg aggtcggcac caacgctctc ggcaagcacc
     7441 gccgatacgg cgtcagtcgt ctgtgcagga gtgatcccgc ccatgaccac gatctttccg
     7501 ctcagcgcga actcaagcgc ctctgtgtag ttctcggcaa cacgcgggta ggccgcctcg
     7561 ccgaggccgg cgatgagaag acgcgcgttc agcctggtca ccatgatgcc gagttcgtcc
     7621 gccgctgcct cgccggcgcc aaggccgcga gcgaccccga tgtacctgcg cgcctccccg
     7681 ccaccgccga caacaatgaa cagccggcat atcccggaga tctttttcag aacggatacg
     7741 tatcgagcga tattgttcga ttcaagcgag gggaccagaa tcgagccgcc cagagatata
     7801 acgatcttct tcatcccacc ctattattac ccggatatca ctcatatacc tgttgtgaag
     7861 atgctgcact gcccggtctg caactcaacc gatgtatggc cggttaccgg aggatacatc
     7921 ggttttctct accgctgcaa gcgttgcggg tatcgcggtg ccctggttgt tgagtatgat
     7981 agagaggctg gagaggtgga taagcggcac ctcccgcaat aactatcatg cttcccgaca
     8041 gggggagatc atatgcatga ggcgcgactc taccagaaac tggaggataa caccgtcagg
     8101 tgctcgctct gcgcccacag gtgcaccatt aaagatggga agcatggtat ctgcggtgtc
     8161 cgcatcaacc gcggcggcac gctgtatgcc gccacattcg gcaaggtgat cgctgaagcg
     8221 gtcgatccca tcgagaagaa accgctgtat catttcctgc cgggaagcct ctcttactcg
     8281 cttggcagcg ttggctgcaa cttccactgt gtgcactgcc agaactggca gatctcccag
     8341 caaactctgg atatgggagg gttcagggat atcagccccg agatgggggt ggagcgggcg
     8401 attgcgtcgg gttctgcaag catcgcctgg acatacaacg agcccaccat atggcacgag
     8461 tatccgcttg agatgggagc gcttgcacgg cagaagggtc ttggaacggt ctatgtcacg
     8521 aacggctaca tcaccgagga ggggttgcgc gacctcgccg gtatgctcaa cgccttccgt
     8581 gttgacatca aatcgttctc tgatgccttc taccggaagg tctgcggcgg aaggcttcag
     8641 cccgtccttg acgcagcgtt gcttgcaaag gaactcggta tgcatatcga gacggttaca
     8701 cttgtcatcc cgggccagaa cgactctatg gaggagatgg aggggctcat ccggtgggtg
     8761 gttgagaacc tcggacccga tacgccgatg cacttcaccc ggttccaccc tgaatacagg
     8821 atgctggatg tctggccgac accggtcagg acgcttgaga aggtttacga acgggcaaaa
     8881 gagctcggga tcaactaccc gtacctggga aacgtcggag gccaccctta cgagagcacc
     8941 tactgcccat cctgcggaaa cctcatcatc gagcgggcgg gctacgccat acggatacgg
     9001 gggcttgaag ggcattcctg cacgagatgc ggggggcata tcgagtatgt ctcggagatc
     9061 cggtgaacgc cgtttcctga ccgacaggat gctcgggacg ctgacgcgct acctccggtt
     9121 catggggtat gacacactga gtgcaaacgg cctctccccc ggcagcacgc gggaagactc
     9181 cctgctgctc gagatcgcgg gtcaggagga tcgtgtcctc ctgacccgcg accgggaact
     9241 cgcgcgccgt ggcggtgagc aggcggtcta catcgcttct gaagatatcc tgtcacagat
     9301 ccggcaactt gccgacgtcg gtctgatcga gccggtggtc aggatgagtc gctgttcact
     9361 ctgcaacacg cgccttcgac cggccacaca gcgtgagatc cgggagaccc catacgcccc
     9421 gccctccccc ccggaaaggg agttctggtg gtgccccgcc tgccggaaac tctactggat
     9481 ggggtcgcat ggggctcgta ttgagcgacg gctgaaagag gtgcgcccgg gctcctgaaa
     9541 ccgggttagc ggggtgcgtg gtcaggcgcg gctgcggatc ctgcaagcgc gaggaagaca
     9601 gcaaacgccg cggagggcga gatcatcccc gcctctatct ccgtatctca gccgaacgct
     9661 ctctttttgg agaacaacgc tgcggtctat cctggctgga gcaggtgtgc gaggcagaaa
     9721 cgggagtccc ggcagcgaga gaggggtata cctggcctaa cagatctgct cgatgaagtt
     9781 ctccaggatc ttcagcccca ccgcaccgct cttctccggg tggaactgga gaccgtatgt
     9841 ctgcccgcac ctgaccgcgg atgcaaacgg gtggatgtag gtggtgctcg cgatggtgct
     9901 ctccggtgcg gccgccgcgt agtaggagtg gacaaagtag acgtactcct cctcatgcag
     9961 cccatcgaag agaggcgccc ccgcctggat gcgtatggtg ttccagccca tatgcggcac
    10021 tttctccccg ccggtcggta caaaccgcct gacctccccc ggtatcagcc cgagcccccg
    10081 gtggagcccg tgctcttcgc tcgagtccat gagcatctgc atacctagac agatcccgag
    10141 cagcggcgtc tcgcgagcgg cgttgacgac tgtcgcctgg agatcaccga gcatctccat
    10201 cccctcgcga aacgctccca cacccgggag aacgattcca tccgctgccg tgatctcctc
    10261 agcatccccc gttgtcctgg gcgccgctcc agcccgctcc aggccgcgaa agacgctgcg
    10321 caggttgcca agaccataat caattataac tatcttcttc ttcatcggaa ctaccttgtc
    10381 ttcgccagag tccagactcg atccagtccg gatcgagccc ctgcttctcc tgccagcggg
    10441 cgagtttctc gtgccacatc ttcatgagtt cgggatgtgt ctccccgatg atcgcaaacg
    10501 tcgcgagatc gcttgagggg cacatgaaac agccgatgcg gtcaagcccc cgctcgtaga
    10561 gacggttgta tggcgccttc tcgcggaaga tgtagagcca gacgtgcatc gccgtccact
    10621 gctggatggg ggctgctgag acctgttgcg ggacgtgcgg gtttcgccag acccgcctgc
    10681 tccgcatccg gcgcaccgac tcgtacttcc gctgccctat gaacgagagg cactcgcccc
    10741 agttctcctc gatcaggcgg ccgacgggat ggagtttgca gacacggcag caccagcggt
    10801 tatcgacagc cggggggccg ttctcctcga acgtctccca gaacgcctct ttgtcacagg
    10861 cgctgaagac ctctagaccg tatcgcctcg ccacttcatc gacgttctca taggtttcag
    10921 ggaactcaag ccctgtatcg gaaaacagga taggcaccgc cccgattgcc tttaagacga
    10981 tcaggagggt tgcaagactg tcttttccgc ccgagtagga gatggtcggg gtgtgcgggt
    11041 tctgttctgc agcctccctg acgaacctga tgcttgcggc ctcgtaatcg gcgaggacgg
    11101 gttcgtttgc cctgacggtg tcctcccagg ccgcctcacc cgggaggggg gcagcatcca
    11161 ggttcttgcg tgtccggacg acgacacccc gctccatcgc tccggcggtg gcggcatcga
    11221 cctttgccct cccgacgccg atacactcgc cgtttgcgct cagtatgaag acctcatcgc
    11281 ccgcggcaac ggccgggtcg atcgagatga tgccgggcgc aagtacgctt gccccctcgt
    11341 cacggatgga cgggactgcg ccatcgtcga ccacgacccg gcgcctggtt ggccgcatgt
    11401 atgccgctgc cgcccgccgc ggcaggggtt cccagcgccg ctctcccggc aggtatcgga
    11461 cggcacccac gacggcgccg ccaaggacga tctcgtccat cctgtccgcc gcaggaacct
    11521 tgttcaggag cgcgatatgc ccttccggta tcaacggcgc cccgaaatga tcgctgaata
    11581 tccggttgat ccgctcgatg tcgtcgggga aggcggggcg tgcgtccccc ggcggtgtga
    11641 cggctaccgg acgggtcggg ctgccgcatg cacaaacccc ggatagcact ggaacgtggc
    11701 aggtgtcgca ccagtgaagc aggatctcgc cgagatagat gcgtggcatt ctcatatctg
    11761 ttggagggaa atggataaaa tattggtggt aatctgcatt tttcgtggat aatccccttg
    11821 tgggcaccat tttccaatct tttcagtcgg gtgcggatcg ggggacagga ttaaatacat
    11881 cctggttgtc atagtattgt catgttcaat catgtagaat tatatctgcc ggagctttcc
    11941 ggggcttttg gcaattctat gctgcgggag tggcagttca ggatcagtct ggacattcaa
    12001 tttccggcag gcgatggtta cacctctact caggtgaggt gtatggggtg agagagcatg
    12061 catgagattg gaagtggagg gatggcatcg atgcggtttg cgcgaaaacc tctgcggaag
    12121 atcgagattc tggggctgat aatattcata ctgatggcag gggtcatgct cgcggtgggc
    12181 gctctgctct tctgcgactg gcttgacgcg ccaggatctg cacctgcagg cagcccggcc
    12241 gatcctggcc cggtattgtc tttcaggagt attgccggtg caatcccgct cattcctctt
    12301 gttgtaggtg tcgaggtcgc aggagctctc tccctgaccg tgctttatct ggtctccgca
    12361 ctcaggcgca ggaagtagcg ctcctccggt gcgctctcct gctgttgtgt ttccttgagc
    12421 ggggaaaaat gcctattcct tctttgaatc cggtgtaaca ctgttgggat acggtaaatt
    12481 ttatatcccg accggtatac atcacgtata tccctccctg ggaaggaggt gtgtcatggc
    12541 aaaggacaag cggtcacagg acaaaaaagc agaagaaaag aaggtagaga agggccagga
    12601 gaaggccaag aagaactaag cctccacata ctcacctcat ctttttttgt gcccggcgtg
    12661 gatgccacag acagtttata tccgtgtgta aggtgtatgg tgtcaacggg tggcataata
    12721 aagcgtattt gctgaaagta caaagatctt gcgttttgga atatttagag atataggggt
    12781 taatgaggat gatcagtaca cgaatgcaat acctattacc acctcagaac aggtcgtatt
    12841 tcgtatgaat aatggcccct ccagatattt tcatctggta tgcctgtggc cccagaggca
    12901 tcatgtagtt ttccagttac gccagagagc cacttcattt cctcttaaat aaactactat
    12961 tgtttgccag caacctgccc cgatccgata tagcaagaca ggtacatttc agcgggggtg
    13021 taaaactccc cattgccgac gccccggcca ccccacagat tgacccccaa cccaaaacaa
    13081 ttaagtgcca gcatggcaga ctatgtaagg taaatcccac cgtaaatccc actgggcccg
    13141 tagcatagct ggtggtgcac ccggctgata accgggaggt cgtgcgttcg aaccgcatcg
    13201 ggcccactgt tccgtatttt gcgttgttta cagtgtttgt tttaataggt ggtgcacccg
    13261 tatgatagcc gggaggtcgt gcgttcaagt tatatcaggt cagttctccc ctcaaccggt
    13321 ctcgtttggc ggtttcgccg accgccgccg gtagagaacg tgctcaagcc cgagcaccgt
    13381 gacgatacca accacaaacc cgtttgcaag caccggccgg agcaccgtag gtaaacccgc
    13441 aacgacctcc gccggcagga acgccaccag cgtcccgagc aggaggggga taccgacgat
    13501 cagcccgcca tcaaacgtct ccagcgcccc gctctcctgg ccaaagacca gccctgccgc
    13561 aatctgggtg gccatgacgt aggccaggac gacgcctatg accggccccg gtatgcactc
    13621 catgcagccg atagcgcgcg gcgatagccc aatcagcgcg agccccacgg ccgccgggac
    13681 gagcgcaaac cgcgaggcgc agcccgtggc cgcgatgaca ccaggactca gcgagaagtt
    13741 caccggccct ataaccccca tcagccccgc aatgacgttg ccaagaccgg tgacacccac
    13801 cccccggtta acccgttcgt ccatcccctc agccgcaaga aggctgccga ccgactggat
    13861 cgacccgaga tcgttcgtgg caagcgccag gtaacagatc agaaatgccg caagcacccc
    13921 gggatcgggg atggcaagcg gtgcagccag atccccgggc agagcaaaca gcgccgtatc
    13981 ggcagggaga gcggcggcgc ccccgaaaag agcaatgtag accgcggtgc cgaagacgag
    14041 cccccatacc gccagcgtcg accgccagaa ccccccaaga atcccgtgcg ccgcaaagag
    14101 ggcaagagca aaccccgcgg caaagaggaa ggccgctgtc gccgtaaccc catcaccacc
    14161 ctccgtgaca agacccagga tgacgggcgc aagggtgaat gcaatgagca ccaggacgac
    14221 cactataaca cgcggcgtga agagccgttt gaggtgcccc acaaaccccg tcagcgcgat
    14281 cgcgctgagc aaaagccccc cgatcagaat cgaggcgttg atggcggctg aacccgcatc
    14341 ctggcttgcc aggataccga caaggagcac cgtcgccggg ccgagaacga tcgggagcct
    14401 gtgacccatg tagacctgca ccaccagcag caccgcgacc aggaggaata acctctgcag
    14461 ataggggatc gccgcacccc cagcctcgag accggccacc accctcccgg cgatcaggat
    14521 gaccgggacg ctcaccacca gccactgcag cccgtagacc aggagcagcc ccggccgcgg
    14581 ccgctcgtca ataccgcagg aaaactccat tcacaacccc ccggttcaag gagtatgtgg
    14641 gcgcttcccc aaatatagcc cttccccgac ccaaaaactc agttatttta tgtctcaacc
    14701 cgataacatt tcacacgatg catctactct atatcgcggc cagaacccgg gtccgccgga
    14761 gatggcggcc atgaacgccg tcgacgtccg cgggctctcg cgttcgtttg acggccggga
    14821 gatcctccgc gacgtcacct tctccgtcgc ccacggtgaa atcttcggct acctcggccc
    14881 caacggcgcc ggaaagacca caaccatccg gatcctgctc gggctcctca cccccggcgc
    14941 cggtgagtgc cgggttctcg gccgcgacct cgccatcgac gacgctgccc gcgcaaaggt
    15001 cggcgtcctc ttcgagaaca acggccttgc cgacaggctg agcgccgcag acaacctggc
    15061 ctactacgcc gggctctacg atctcgacga cccccgcgac cggatcgacg aactcctggc
    15121 gctcgtagac ctcgaggacc ggcgaaacga ccccgtcgga acgttctcga ccggcatgaa
    15181 acgaaggctc gggatcgccc gggcaatcct ccaccggccc gaggtcgtct tcctggacga
    15241 accctcgtcc gggcttgacc ccggtgcgca gcggatggtc cgcgacctca tagtcgacct
    15301 ctcacggcgc gaagagatga ccgtcttctt aaactcccac cacctcgacg aggtccagcg
    15361 gatctgctcc accgtcgcga tcctggcggg cggcaggatc cgggcgttcg actcggtgaa
    15421 gaacctcacc gccgcctccg gcagcccaga gatgacggtc gtccttgccg acccggacga
    15481 ccgtgcccgc gcccgcgaaa ccgcaatggc gctcccttac gtcagcggct gcgaggagga
    15541 tggagaggtc ctgcgctgca ccctcaccga ccccggcgtc atccccgacc tcatcgcggc
    15601 gctcgccgcc gcagggttca ggctccttga ggttcgccgc tcttcgcggt cgcttgaaga
    15661 gatctacctc gaaaacgtcg gccagtcgga gggcggctca tgagcacgtt taccgtcata
    15721 gccggccggg agattcatat cgcgctgcga aaccggggca tcatcatcac cgccctcatc
    15781 ttcgccgtat ggttccccgc cctctcggcc gtcgggatgg tggcaacttt cggcgggggc
    15841 gccgaggccg tctccggcgc cgttgccgcg gtcaccctcc ccgtagccat cctaatgggc
    15901 tacatcttct gctccgacgc cttcctccgt gagaagcggg acggcagcat cgagacgctc
    15961 ctctgcgccc ccgtctcgct ccggcacctc tgggcgggaa agacctgcgg agtcgccatc
    16021 ccggcctacc tgacaaccct cctctcagcc gctgtgacgc tcgcgatggc ctcagccatg
    16081 ctggaggctc cacccagcct cgacctcctg ctcatccccc acatgctggt cgtcgtcccg
    16141 gtctggatcc tcaccgccgc cgggttcgtc ggcgccgccc agctcgcgct cgggatgcgg
    16201 gagaaccaga tcctgggctt cctcttcata ttcgggttca ttttcctaat cggcgggctc
    16261 cagggcatca tatccggagg ggcggagatc tcggccgcgg ccgaagcggc tctcgcggca
    16321 gcaggggctg cgctcctcgg tcttgcccgt ttcatcgccg gaaaggtatc aaaagagagg
    16381 atcgttacga cgatcccgtg aagggagggg gaaaagaccc tcctacagcg tctcctcgcc
    16441 gtagaggtag ggcctcaggg cctccggcac ggtgaccgag ccgtccgggt tctggtagtt
    16501 ctccaggatc gcccggatag cccgtgttgt cgctatagcg gtgctgttca gggtgtggag
    16561 gtagcgtttc ctcgtgaact caacagggtc gcgcaccttg atgtttagcc ggaccgcctg
    16621 gtatgccgtg cagttcgagc atgataccgc ctcgcggtag cgctcctccc gcggcatcca
    16681 gacctcaagg tcatacttct ttgaagcgac cgtcccgata tcccctgtac atatcaggac
    16741 aacccggtag ggcagaccga gccgctggaa gacctcctcg gcgtttgcta tcagctcctc
    16801 gtgaaggtcc caggactgtt ccggggtgga gtagacgaac tgctccacct tgtggaactg
    16861 gtggacgcga aacagcccct ttgtatcgat cccgtgcgcc ccgatctcgc gccggaagca
    16921 ggggctgata cccgcaagcc tgagcgggag gtctttctcc tcgaagatct caccgctgta
    16981 catcgcggcc atgggatgct cgctcgtcgc gatcaggtac tcgtcctcgc cgtcgatctt
    17041 atacatcacg ttctcaaagt ccgcaaggtc ggtcaccccc tcgtatgagg cccggttcat
    17101 catgtagggc gggatgatcg gggtgtagcc gcgcctgatg aggagatcca tcgcgaaccg
    17161 ctggagagcc atatcgagca gggcaagcct gcctttcagg aagtagaacc ccgcacccgc
    17221 gatctttgcc gcacgttcga agtccgccca gtcatgctcg acggcaagcg tcccatggtt
    17281 caccaggtca aactccggga ctttcggctc cccccagcgc cggatctcga cgttcccggt
    17341 ctcgtcactc ccgaccggca cgctctcgtg gaggatgttc gggaggcgca tcagccggta
    17401 gcgcaccgct tcggagaggc ggccgcgctc agcctcggcc tccctgatcc gctccggcag
    17461 acccgccgcc tcggagagga gcggcgatat atcctccccg gccttccttg ccctgctgat
    17521 ctcgcgggag acaacgttcc tgcggttgcg caggttcccg atctccaccg tgagttcacg
    17581 gacccggcgg tccatctcca gcacctcgtc aacccagggc agtttatcgg tatcccctct
    17641 cttcatgagg tcggccctga cgatctcggg gtgttcgcga acgaacttca gatcaagcat
    17701 attctttctc tctacttact tcggcatgac ggtatatggt catgttgcac atcaacgccg
    17761 gccgctccga aacctactct tataatcctc ctgcggatca atccataatc atggacagga
    17821 accagaagat cgttctggtg gccggcggcc tcataacgct cgggctctta ttcatcgact
    17881 acttcgtcgc cctgatcgcg ctggtctgcc tgctcaccct cctgatgtgt ctccatatca
    17941 tgagggagac cgcagacttc ccgcttgtca ccgccgacct atcagaagac gcccgcagga
    18001 tcatcctcac caacaccggc accgccgtgg cgaacaacat ccatctcgcc ctggtccccc
    18061 tgaatatcga gtttgatcac ccttcgctcg gccctgacga gaggtcaagt ctagaacttg
    18121 agtcgatggt ctcagaagca aaagcggtcg tcacgtacga gaacgccggg ggagagcggt
    18181 tccagcgctc ctacccgctt acggcgctcc gtgccggaca cgaccctact gaacccatgt
    18241 tcccggtctt cggaaaacgc tgagacgctc tcacggaata gcgatgtact ccagcgtccg
    18301 cgcgagggcc gccatgagat cctcctcctt ctccgcatac tcgtagagcg cccggtagag
    18361 catgaagagg atctcgtagc catagatctc gaacgcctca tccggcgtca gcacagatag
    18421 gtaggagtct gtccggatct cggtgttggt gtacatcagt tcgtggaaga ccccgtcctc
    18481 gtcgagtacg cagatctgca catcgaccgg ctttgccgta tcgtcggggc ggtaggggag
    18541 cggctccccc ttcccgagga gtatcatcct cttctcataa aactcctggt cgtagagctg
    18601 ccccgaggcc tccctccttg cacgccggag catctcaagc ccgaccttct ttaccgccgg
    18661 cgctgcatcg gcggccatcc ttgcaaggag agttgctgcg ttctccctga ccttcgcggc
    18721 agcctcttcc ttctccgcct ccagggtgtc cagggtctca aggagccggt tatacccctc
    18781 ttcgatgatc ggatccattg cttcattcac cgttttgcgt atctctccat aagcgcaccg
    18841 atctgcctcc acggatcccg agcgcggccg cgtatgccgc cgtgctcacc aggatgatcg
    18901 tcgcgccgga ggggacgttc aggacgtagg agaggccgat cccgatcagg gtgaagaggg
    18961 cgccgaggcc ggttgcaagg agcatcatgc tccatactct cccggtgtat agtctgctga
    19021 tcgccgccgg gatagtcagg agagcgatca ccaggatgat cccgacgacc cggatcagca
    19081 tcaccacagt gagcgcgacc agaaccagga ggaggagcga gagccgttcc agcgggaggt
    19141 tcatgaccct tgcgtagtcc ggatcgaagg ttaccgcctg gaactcaagg tagaagaggc
    19201 cgacgatcgc gacgatgatc gccaccagca ccgccatcag gaggatatcg ccccgcggca
    19261 ccagcaggat gttcccgaag aggtaggaga ataggtccgg cgcaaacccg ggcgtcaggt
    19321 agacgaagag tatcccgaga gccatcccgg ccgcccagac cgcgcctatg atggtatcca
    19381 tctgctgccg ggcgcggatc tcaagcaccc ccataccgag cgccgtcgcc acggtaaaga
    19441 ccgtcgcgcc gaggagcggg tcgatcccga ggtagtagcc aagccccaca ccgccaaacg
    19501 ccgcgtggga tatccccccg ctgacagaga ccatccgccg caccacgaca tacgtcccga
    19561 tgatcccgca ggcgatgctt gcgaacaccc cggcaagaag ggcgttgcgg aagaactcga
    19621 acccgagcac ctcaagcatc ctctccgccc tcccccgcgt ggtccgggaa tacccggtgc
    19681 ggaaccccgt gggctatcag gtccacgggg cagtggtatg cggcctcgag catgtccggt
    19741 gtgatctcgg ccgagtcgtg ggtgtagagg cgccggttca ggcaggcaac gcgagtgatc
    19801 cgatccggga tcacccctat atcgtgggtg acgaggacga tcgccatccg ctcgcggagt
    19861 tggtcgagga tctcgtagaa ctgcagggtt gtcggggtgt caacgtagac cgtcggttcg
    19921 tccagaagga ggacattcgg ctcgccgacc agcgcacgtg cgatgatcgc gcgctgctgc
    19981 tccccgccag agaggtcgcg gatctggcgg tcggcaaggt ctgagatgcc catcgtctcg
    20041 agcgcttcat ccgcccgttc gcggtccagg cttccgtacc gccgcgggag acggctgatc
    20101 cggccaaggc ggccggagat caccatctcc cgcacggtga tcgggtaatc gaagtcaaac
    20161 gtccggaact gggggacgta gccgagactg ctcataccgc cgcgaaacct gacctccccc
    20221 cggcaggggc ggaggagacc gaggatcacc cggagaagcg tagtcttccc gccgccgttc
    20281 gggccgatga tggcgaagaa gtcgtcaggg tagatgctga ggttcaccgc ctcgagtacg
    20341 gtatgcccgc gcatcgtgac ccagacgtcc ctgacctcga tcagaggttc gctcatccgc
    20401 tgacgctccc ggcgaaggcc gcggccacct gccgcatgtt atcgaggtag tccttcttga
    20461 gcgggctcac agtcaccacg ctgccgccga tcgcctccgc gatcaccccg gcgctccggg
    20521 tggagtgctc cggtgaggcg aacaccaccc tgacaccctc ctcctctgcc aggcggatga
    20581 ggtgctctat cctctttggc gagggttctt tgccctcgct ctcgatcgcc acctcctcaa
    20641 acccgtagtc ccgggcaagg taggcccagg aggggtggtc gaccagcacc accctcacac
    20701 ccgatccggc gatcagagcg gagatctcgg cgtccagggc atcgaggtca tccaggtatg
    20761 ccgcggcgtt ccggcggtag tcttcggcgt tctccgggtc ggcctcgata agcccgtcgc
    20821 ggatattctc gaccatgacc tttgcgttcc ggggtgatgt ccagatatgg ggatcggccg
    20881 cgataacctc gaccccctgc gagcagttca ccacagtcat atcggggttc agggcggcga
    20941 tcttctctcc ccaagcaatc tcaaactcga tccccgaccc gaccatcgca tacagatccg
    21001 cctctgcaac cgcggccagg acggccggcg cgggttcgta ggtgtggggg tcggcaccgg
    21061 gaggcacgag caggatcacc ctgacatgat ccccgccaac ccgctccaca aactcctgct
    21121 gaggggggat ggtgacaacc acagcaatcc ggccatcatc ctccacatcc ccgtcagcgc
    21181 cggtgcaccc cgccatggcg gtcatcagcc cgaggaggag gcatgcgacg gcaacgaggg
    21241 ataatggttt gatcccggtg gtatccagca tcatactcat ctccataatt tggtacctat
    21301 ctaaatcttg ctcacaccaa ccgtccgccg gaggagcagc aaccgtgatc gtgctcgatc
    21361 tcgccgcaga cgctcatcat cgggtgcccg agcgaccagc agatcctgtc gaccaggtcc
    21421 tttgagaaag actgctcgat cttctccgcc tcactgcagg cctcctcggg cgtaagcctg
    21481 tagtggctca gcaccagcgc aacgatacgg tggcggcgga ccagaaaccg ggcatactcg
    21541 acgccgggag gggtgagtct cacgccgtgg tagggcgtgt gctcgatgta accagcgttc
    21601 gcgatctccc ttaaggtctt tgtgacggtc gatggggcaa cgtcaaaccg ggcggcgatc
    21661 tcggttgtct tcacgagatc gccccgcatg tagatgtact tcaggtagtc tgccttcttt
    21721 gaggagagtt cctggccggt gaactcctgc atgagagagt gtctattcaa ggtatgagaa
    21781 aaagtttccg ttgggctaaa cctggaccgc cggatcaagg cttccatcca ggatatgggc
    21841 tcaccacgtt ttgcaggccg cagggacggg ggcgcttcaa accaggtttg ctttaacttg
    21901 cgggggtgaa gggggcgggc ggggagtgat gattggccta tggaagccgt gtccggggac
    21961 ggaaaacggg atctgcgagg agtttcgcgc ctgaaaccgg ctgaggtgat gctctccccg
    22021 gacactgtat cgatggggaa tcgccaatag ggggaggggc tgacggggag ggggatggaa
    22081 catcccccgc ccctgtctac tgcatctcag gttgaacggt ttacgtagag acacccccac
    22141 cgcccggccg ctaccgcggc ctcaaaccag ttccccgatg acgtagtggc ggtggttccc
    22201 ggttatgagc
//
LOCUS       DAOSZX010000014        22158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1927_length_22158_cov_19.874542, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000014 DAOSZX010000000
VERSION     DAOSZX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22158
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_22158_cov_19.874542"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK196_02325"
     CDS             <1..243
                     /locus_tag="PK196_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HON40293.1"
                     /translation="HHVLRENKQATGAGADRVSEGMRLAFGKPVGTAARVREGQKIFS
                     VWTTPQFVEKAKASLKGGTYKLPAPARIIEERVQTA"
     gene            341..1180
                     /locus_tag="PK196_02330"
     CDS             341..1180
                     /locus_tag="PK196_02330"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HON40294.1"
                     /translation="MMVNQADPGLVEALADATERALREAETRLPPDVLAALRRAAAAE
                     TNGIARQELENILENIALAGERGLPICQDTGVPVVYLSLPPDVPFSPALFEGVREGVR
                     RATAAIPLRPNAVDPITRQNTGDNTGAGMPPVHVVPGERLTVTVLPKGAGSENVSRIG
                     MLLPSQAAGIPEFVAETMLIAGGRPCPPVILGVGIGGTFDAAAALAKEALLLPADTMD
                     EYEQELCDAVNALGIGPMGLGGDVTALAVKVKRAACHTASLPVAVNVQCWASRRASVE
                     VRL"
     gene            1138..1734
                     /locus_tag="PK196_02335"
     CDS             1138..1734
                     /locus_tag="PK196_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HON40295.1"
                     /translation="MLGVPPGERGGETLNLKTPLGDEVLDLRAGDRVSLSGIIYTARD
                     EAHLRMMEEGIPFDPEGAAVYHCGPVVQDGRLLAAGPTTSARMNSLSGFLIDAGVRAL
                     IGKGGMGQEVVERLRGRGVYLAFTGGCAALAAARMRITGVYFEELGMAEAVWVLEADS
                     LPLTVGIDAHGGDLFAAAQEKAKTKFQQRFNIKQDTFS"
     gene            1731..2003
                     /locus_tag="PK196_02340"
     CDS             1731..2003
                     /locus_tag="PK196_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HON40296.1"
                     /translation="MKLVIDETRCKGCNLCVLVCPYRIFKEGTELNDRGIVTPVLDRP
                     ERCTNCRLQPLYGRLLCGVCQMICPDQAIAWVDERPFEPHKVEVEF"
     gene            2000..3100
                     /locus_tag="PK196_02345"
     CDS             2000..3100
                     /locus_tag="PK196_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HON40297.1"
                     /translation="MSRTEFMQGNVACAEGALAAGCRFFGGYPITPSTEIAETMARRL
                     PKAGGVFISMEDELGSIAAVIGAAWTGARAMTATSGPGFSLMMENIGYAAMTETPCVI
                     VNIQRGGPSTGQPTRAAQGDMLQCRFGSHGDYSTIALSPSSVQEMFDLTAKAFNLADR
                     FRVPTFLMADEIIGHMRERMTIPDKVEIAGRPPLPEGRLPFEAGEDGIPGFAPFGSGR
                     AIHVTGLTHDERGYPDTTDPEAHERLVRRLVAKVESARREIADYEVVNPDARVVFVTY
                     GAPSRTVQQVIHDHPDEGIGHLRLRVVWPFPEFALAEFPDARVFLMPELNMGQISREV
                     ERHVGQPVVPIPKLGGDLHTPADLLRALEAYL"
     gene            3097..3885
                     /locus_tag="PK196_02350"
     CDS             3097..3885
                     /locus_tag="PK196_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON40298.1"
                     /translation="MSRAEWLREDRLPHIYCSGCGNGTVINCTLAAVDEVGWRRDETV
                     FISGIGCSSRAPGYINTDSLHTTHGRALAFATGVKLARPDLKVVVFTGDGDLAAIGGN
                     HFIHACRRNVDMTVVCMNNQIYGMTGGQGSPTTPPGAISTTTPYGASEPAFDLADLAI
                     AAGANYVARWTSYHVTELTKAIATGLQTPGLAFIEVRTQCPTNYGRSNRLREVSDMIE
                     YLRSHAMLVQKYERLIAEGKPIPEGTFTIGELVRRNRPAMGVQP"
     gene            3882..4421
                     /locus_tag="PK196_02355"
     CDS             3882..4421
                     /locus_tag="PK196_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON40299.1"
                     /translation="MRHEVRFSGYGGQGIILSSVILGRAAALYDGKYAVQTQVYGPEA
                     RGGASMGQVVIDDEPILFPEVTDPDIYVIMSRQGFEKYGVCARKDAFMLIDADLVRSR
                     PGCRCCEIPATSEAKTNLGREIVANIVMIGALVAATGVVSREAIERAVLDSVPKGTEG
                     LNQRALKRGFELGERACNP"
     gene            4418..>4683
                     /locus_tag="PK196_02360"
     CDS             4418..>4683
                     /locus_tag="PK196_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HON40300.1"
                     /translation="MKLLEYEAKRVFSDYGIPVPQGFVIREPGEIAAHLSEFGDAVVL
                     KAQVDVGGRGKAGGVLVVDSASAVDRARELFSREIRGVPVREILV"
     assembly_gap    4684..4783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4784..5287
                     /locus_tag="PK196_02365"
     CDS             <4784..5287
                     /locus_tag="PK196_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit beta"
                     /protein_id="HON40301.1"
                     /translation="YAADAKLIIDDNALGRQGIEVNRDLSEREREAGRHGFSYVELDG
                     TIGVIGNGAGLTMSTIDLIDYYRGRAANFLDVGGGADRERVMHAVRLVAGLPSVKVIL
                     VNLLGGITRCDEVAHGIIAADVGVPVIVRMAGTNEEEGRRLLAEHGYRMVDSMEAAVR
                     AALEAGE"
     gene            5284..6150
                     /gene="sucD"
                     /locus_tag="PK196_02370"
     CDS             5284..6150
                     /gene="sucD"
                     /locus_tag="PK196_02370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HON40302.1"
                     /translation="MIYGDRNLGVIVQNITGRQGRFHTELMNAYAREVGWKGIVAGVA
                     PGKAGMEVDGVPVYNTVKEALAEHDAAASVIFVPAPAAADAIMEAAYAGIELAVVITE
                     LIPVHDTMKAIAYAKLHDCAVIGPNCPGLLSPGECKLGIMPAHLATRGEVGVVSRSGT
                     LTYEVVDELSRAGIGQSTVVGIGGDPVIGQTFVDVLARFEEDAHTKAVVLIGEVGGNL
                     EEEGARSTDLPVVAYIAGVSAPPEKRMGHAGAIIEGGEGDARSKVRRLSEMGVPVAAR
                     PSEIPGLIREVL"
     gene            6147..6950
                     /locus_tag="PK196_02375"
     CDS             6147..6950
                     /locus_tag="PK196_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HON40303.1"
                     /translation="MNGELMLATACEVAKKIGARAVVSFIEPAPVMAEPPGIPIIRVQ
                     ELQLDVLKDLTMHDILEVSQRHLLDAAVHLYLQRNFESGLIVGVFSYAIVIYDIEEGK
                     NFISLKDFSDIVPREVLHAVLTLALEIAVEGREGRAIGTAFIIGDPDEIFRHSHQAIL
                     NPYHGHPPALRDIKKRDNWESVKEFAQLDGVFVIDKNGEVRAAGRYLDVSGRGISLPS
                     GLGGRHRATASITREIPAIGITVSESGGVVRLFREGECKLSIRSDIRLR"
     gene            7020..8222
                     /locus_tag="PK196_02380"
     CDS             7020..8222
                     /locus_tag="PK196_02380"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HON40304.1"
                     /translation="MTRNISVEGLEQIPIEKQRIELVERKCLGHPDSLADGIAESISR
                     ALSRSYMEECGSVLHHNTDQGEVVAGESLPKFGGGIITRPIYFLISGRATKTFNGVNI
                     PADAIAVEAARDYIRKTLPMLNMERDVIVDCRMGRGSTDLQDVFRSCSGKAPRANDTS
                     FGVGHAPFSEAESIVLGISEFMDRTLRPKHPVIGQDCKIMCLRDGDTINLTLAMAFVD
                     RYCSGIADYIEQKNFLTEQIAAVAGQFTSRRVNVAINTADDLDNCSVFLTVSGTSAEM
                     GDDGSVGRGNRANGLITPNRPMSMEATSGKNPINHIGKIYNLLATEIAKDCVARVDGI
                     EEIYVRILSQIGHPIDQPHVASAQILPKSGFEVSALRPEVEGIIDDWLERITTITEKV
                     IRGELSTF"
     gene            8256..9152
                     /gene="hisG"
                     /locus_tag="PK196_02385"
     CDS             8256..9152
                     /gene="hisG"
                     /locus_tag="PK196_02385"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HON40305.1"
                     /translation="MSKPVPRSPDPGFIRLAIPNKGRIAGPVHELIEKSGLHLADGGG
                     ERRLIARTRDPHVEILFARPIDIPEYVATGVADLGITGRDMVLERGSEVKELLDLQTG
                     RATLVVAVPEESDVESITDLDGARVATEFPGITRAFFAEHGVAVTVVTVGGACEATPH
                     LGIADAIVDLTSSGTTLRTNHLRVVQEILTSTTILVANPRSLKTRGEKIDEVVLALDS
                     VLQAKGQCYLMMNVHRSALADVREILPGLSGPTVMDVASDENLVAVHAVVNEERVYQL
                     INQLKRAGAKDILVMPIERIIR"
     gene            9157..9876
                     /gene="hisA"
                     /locus_tag="PK196_02390"
     CDS             9157..9876
                     /gene="hisA"
                     /locus_tag="PK196_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HON40306.1"
                     /translation="MEIYPAVDILEGRCVQLVQGRRDAATVYGDPLAWARRWLDLGAD
                     GLHVVNLDGAFGKAKRNADLIRRLAGETETFIELGGGIRSVEDAAGWLDAGVDRVIVS
                     TLAVREPEVVRRLADEFGSERVMVGIDARGGEVVIEGWERPAGNYLSWAERFESLGAG
                     SLLYTNVDVEGLQQGIDIAPIEALLARVSVPVVVSGGVSSARDVCALRAAGAAGAVLG
                     SALYSGRIRLSEAMEAAHAKN"
     gene            9863..10447
                     /gene="hisB"
                     /locus_tag="PK196_02395"
     CDS             9863..10447
                     /gene="hisB"
                     /locus_tag="PK196_02395"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HON40307.1"
                     /translation="MRRTEIHRKTRETDIRLSLDIDGTGTARIETGIPFFDHMLESFA
                     RHGRFDLGIEAAGDLAVDAHHTIEDIGIALGKALAAAVGEGKGITRFADAAVPMDEAL
                     ARVALDLGGRGYLVFEGGFSPAGPGGIPGDLIEHFFYSLCTNAGLTAHISLTGRSDHH
                     VCEAAFKAFGRALRAAVAIDPAMGGEVPSTKGTF"
     gene            complement(10801..11007)
                     /locus_tag="PK196_02400"
     CDS             complement(10801..11007)
                     /locus_tag="PK196_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HON40308.1"
                     /translation="MADLPIAAVVRIAKKNGAERVGSDAAAALVAKAEAYIAELTREA
                     NRLAQHAGRKTIKAEDVELAVKSA"
     gene            complement(11193..11954)
                     /gene="mtxX"
                     /locus_tag="PK196_02405"
     CDS             complement(11193..11954)
                     /gene="mtxX"
                     /locus_tag="PK196_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HON40309.1"
                     /translation="MPIKIGLGAAKAEAKILSAARAVRGEVNLVLFAPPGTADSFGDE
                     VTVIEAGEPEAAIVEALYTGRIDAAVRGTLPASTTLKALKRAAGVDRLERIALLETPD
                     GHLFLLAPVGVDEGWTVEQKVRFASAGRDIARRFGLPEGVAILSGGRLGDVGRHPAVD
                     RTMADAELVARLSGAEHIEILVEEVPGRYGLVIAPDGITGNLIFRTLALLGGGRGHGA
                     PVVNIDRIFVDTSRASADYTNAIMLAKSLVLPEKP"
     gene            complement(11945..12955)
                     /locus_tag="PK196_02410"
     CDS             complement(11945..12955)
                     /locus_tag="PK196_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HON40310.1"
                     /translation="MVKDCSTDTVAQAVREYEGVTRKREIGDMIRALRVDSPDVVASF
                     GEDAAVIRHNAEDALLLAADGIWSRLMEADPFWAGYCAVLVNIHDIAAMGGRPVAMVD
                     ILSAVNDQIRDEVVRGMVTASSQFGVPIVGGHLHPDTPYSVVDVAILGTARMSEIIFS
                     HTAEEGDSVVAAIDLDGRVHPSCCFNWDSVTMKSAAVVRAQILVMEDLGREHLVTAGK
                     DISNPGVIGTLGMLLEVSGKGAVVNLESIPRPDLAALGISFEHWVRMYPGMGFILTVK
                     PENVDEVCRRFADVGMAAAAIGEVNGDRRLTVRYLGRETQVFDLDRDGIMRIFAGGGG
                     CR"
     gene            13173..14534
                     /locus_tag="PK196_02415"
     CDS             13173..14534
                     /locus_tag="PK196_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HON40311.1"
                     /translation="MDWAEKYRPQHLNEVVGNSSAIRQMYEWARDWSPEKKPLILYGK
                     PGTGKTSSAYALANDMNWEVVELNASDQRTKAVLERIAGSSSTTASLTGASRKLILLD
                     EADNLHGQADRGGAKAIIDIIAASQQPIILIANDYYALPKELKAVTEPVQFRALQSRS
                     IVPRLRQICAAEGVECDPAALEEIANRADGDMRAAVNMLYAAAIGKDRLLAGDIHTST
                     KDERSTIFELVGAVFAGRKDADLLRLVMEVEDTPDTVEQWLEGNLDHIPDLPSRARGY
                     AYLARADEYIGRTYQRQYYTLWRYANSVMLLGVAEAAGGRGVRGRIMPPSRWQKMGAS
                     KKQKAVRAGLYRRLSGILKIPEEALRESFFTAISILVERDAARYVREFGLDADELNLF
                     INDKPRAARVVREAAKEEKEAKKTPPAKKGGGRAKKPAEDAPKDEPGEARRGPPPGQA
                     TLF"
     gene            complement(14531..14938)
                     /locus_tag="PK196_02420"
     CDS             complement(14531..14938)
                     /locus_tag="PK196_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HON40312.1"
                     /translation="MGQYKHIERSIGEYIASRYRRAVEVGIGNNPVAAGVVAAAGALM
                     LCTDIRPGIQHEGLAVVTDDIFEPDMRLYLGADVIYSVRPGVEMVPPLISLATRTNSD
                     LLVYHLGCEVYRDGGEVVDAGGVQLHLYHRRRP"
     gene            14956..15495
                     /locus_tag="PK196_02425"
     CDS             14956..15495
                     /locus_tag="PK196_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HON40313.1"
                     /translation="MGIHILWDTEAPVGIELPVIRMIAMILGEESELVEYPLLIDGYD
                     RERNQHDAHKILDRLQDTLTRRYDIDGPLLFVTSRDLYIGGCDFVFGLARPRAGVAVV
                     STARLGNDYYGRVPDDTDLIDRTAKEGAHELGHLLGLGHCSNPECVMFRPQTLDELDR
                     KKKVLCPVCREALAPLQEG"
     gene            complement(15509..16318)
                     /gene="budA"
                     /locus_tag="PK196_02430"
     CDS             complement(15509..16318)
                     /gene="budA"
                     /locus_tag="PK196_02430"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HON40314.1"
                     /translation="MTNGRLLRFIIVAAILITLGACIGFAAVPLQSTAETDRETLFQV
                     STIDALLQGIYDGSMSFDELASHGDLGIGCGDRLDGELIGLDGGWYLIRVDGRAYPVA
                     GNATTPFAATTFFDPDIEIAVEKPMTLPDLENRVRDEFPSENYFYAIRVDGTFSQVVT
                     RSVPAQEKPYPRLALVTANQTVFSFKNITGTAVGFWSPGLAKGVNVPGYHLHFITTDR
                     TAGGHILDMTLAEGTVRVDTTKNFSVVLPSSSDFLAVDLTEDLSEDLGQVE"
     gene            complement(16326..16760)
                     /locus_tag="PK196_02435"
     CDS             complement(16326..16760)
                     /locus_tag="PK196_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HON40315.1"
                     /translation="MDLFMKCSIEEAEAGLREGGIPIGSVLVRNGQIISRGRNRRVQE
                     NNPILHAEIDCLINAGRIRNYRECTLYSTLMPCYLCAGAVVQFGIRKVVAGESANFAG
                     AREFLESHAVEVIDLDLEVCKEMMAAFIREHPDIWYEDIGGT"
     gene            complement(16899..17435)
                     /locus_tag="PK196_02440"
     CDS             complement(16899..17435)
                     /locus_tag="PK196_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON40316.1"
                     /translation="MDLPVPEEEETGEKSVERAFNRHISMLRWGGLNERWRAADALAE
                     FADDRAVEPLIEALDDDYADVRWRAATALGILDGREAVTPLIHCLEDESSWVRMAAAR
                     SLGQIGDPRAVDPLIRLLGDEKPRVRREAALALGRIGNPLARDALLRLLDDIDRDVRG
                     AARQALAEIGIRGEASTA"
     assembly_gap    17535..17634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17685..18116
                     /locus_tag="PK196_02445"
     CDS             17685..18116
                     /locus_tag="PK196_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40317.1"
                     /translation="MDSPRTVKPEATAFPPELERLGIAAGARIDIRDLDALSKHHNFQ
                     AYLYFEEDLARGSTLGRDLEDYRRVPEVERPFVNLDEFLRFATASDPLFSSYLDELPL
                     VIEILACGEIHAEDGEAVAYVKGLMPFLDELDVDDALPGAS"
     gene            complement(18272..19126)
                     /gene="nadC"
                     /locus_tag="PK196_02450"
     CDS             complement(18272..19126)
                     /gene="nadC"
                     /locus_tag="PK196_02450"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HON40318.1"
                     /translation="MIPIDDLLRFVREDAPWGDLTSETVVPDVVCRAVIRAKGSGVIA
                     GLKEARALFEHFGVTVYLHTADGREVAAGTAIMDLEGPARAILLLERTALNIIGRMSG
                     IATATREAVERVSRVSKGVRVAATRKTAPGLRVLDKKAVILGGGDPHRCTLSDMILIK
                     DNHLALVPLPEAIRRAKARSRYLMIEVEVETVEDAVTAAEAGARVIMLDNMTPEDVKA
                     AVEALKREGLRERVTLEVSGGVDGADLAVYAATGVDIISMGALTHTVRNFDVSLDILK
                     GVFTVRLE"
     gene            complement(19132..20028)
                     /gene="nadA"
                     /locus_tag="PK196_02455"
     CDS             complement(19132..20028)
                     /gene="nadA"
                     /locus_tag="PK196_02455"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HON40319.1"
                     /translation="MEDEIRALKSRKNAVILAHNYQPMEIQALADVVGDSLELAVKAK
                     ETGADLIVVCGVRFMAETAKILNPGRKVIIPVEDAGCPLADHLTPAMILEARRRHPGA
                     AVVVYVNSSAECKALADITCTSANAVRVVASLQNDEILFGPDANLAAYVQRELPDKRI
                     IPLPPGGHCYVHTGFSLADVEAARKRGGIIVCHPECLPEVQEEADMIASTGGMVRGAA
                     DGGNGPWWVFTEREMVSRLRYLYPGRVFYEKPDAVCSDMKKIKPADLLRALDREEHEI
                     VLPPGVMEDARRAIERMLAAGV"
     gene            complement(20032..20790)
                     /gene="nadX"
                     /locus_tag="PK196_02460"
     CDS             complement(20032..20790)
                     /gene="nadX"
                     /locus_tag="PK196_02460"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HON40320.1"
                     /translation="MIKIGLLGCGNVGHIIAAHAGGIQITAVYDIIPERAEELAALCG
                     ARAYTDFEAFMHDEFSIVVEAASINAVRTFGEAILRSGRDIVVLSVGALAEEDFREHL
                     VGVAREEGKKIRIPSGAILGLDNLKIGQVSPPRQLLLRTTKPPASLGMDVAARTELFK
                     GLAHDCIRQFPKNINVAVALGLAAGRDAEVELWVDPAVERNIHEVFVEGEFGDIYIRV
                     SNVPSPDNPATSYMAALSILALLRNLENPLVVGT"
     gene            20935..22152
                     /locus_tag="PK196_02465"
     CDS             20935..22152
                     /locus_tag="PK196_02465"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HON40321.1"
                     /translation="MTLDELRGRRVYIETYGCTYNQADSRRLERILEGQGCTITGRPE
                     EADAVVINTCTVIGRTERKMLRRLEEFADRDLYVTGCMPLVQMDEIRSVCNPRVIHPD
                     EIQERSGSIGTRGPGATGVVQVASGCVGRCSYCITRLARGRLRSAPAEAVLDAVRGLL
                     ASGAYEIQVTGQDVAAWGLDRGESFPDLLRGISGILGRFAIRVGMMHPASVTGILDDL
                     VEAFHSEKVFRFLHLPVQSGSDTVLERMQRGYTAADVIRIVDAFREEFPDMMISSDFI
                     TGFPGETAEEFQETLELLRRCEFVKVNVTRYSRRPGTPAAALKDLPDRLRKERSRALL
                     VEANRIYDRYNERWMGRVTPVVATEKNVPGSTVCRNPCYLNVVIRDDLPPGFSGRALI
                     TGNHRHYVIGELV"
BASE COUNT         4278 a         6730 c         6935 g         4015 t
ORIGIN      
        1 caccacgtcc tgcgcgagaa caagcaggca accggcgcag gagccgaccg tgtctcggaa
       61 ggtatgcgcc tcgccttcgg caaacccgtc gggacggcgg cacgggtgag ggaaggtcag
      121 aagatcttct ccgtctggac aaccccgcag tttgtggaga aggccaaggc ctcactcaag
      181 ggcgggacct acaaactccc ggccccagca agaatcatcg aagagcgggt tcagacggca
      241 taacccccca tatccacctg tttctgagtt tctccctgca gggagaagat ccctgcctgc
      301 ggggaatata ggtttttata gtctcttccc accagtatag atgatggtca atcaggcaga
      361 tccggggctt gtggaagcgc tggcagacgc aacagagcgg gcgctccgtg aggctgagac
      421 ccggctcccc cccgacgtgc ttgctgcact ccggcgggct gcagcggccg agacgaacgg
      481 aatagcgcgg caggagttgg agaacatcct ggagaacatc gcgcttgccg gagagcgcgg
      541 tctgcctatc tgccaggaca ccggtgtgcc ggtggtctac ctgagcctcc cccccgacgt
      601 tcctttctca cccgcgctct ttgagggggt tcgggaaggc gtgcgccggg cgactgccgc
      661 gataccgctc cgtccaaacg cagtcgatcc catcacccgg caaaacaccg gagacaacac
      721 cggcgccggg atgccccccg tccacgtggt tccgggcgag cggctcacgg tgacggtgct
      781 cccgaagggt gcgggctctg agaacgtctc caggatcgga atgctcctcc cctcgcaggc
      841 ggccgggatc ccggagtttg tcgcagagac gatgctcatc gcggggggaa gaccctgccc
      901 gccggtgatc cttggcgttg gcatcggcgg aacgtttgat gcagcggcag cgcttgccaa
      961 ggaggctctc ctcctcccgg ccgataccat ggatgaatat gagcaggaac tctgcgacgc
     1021 cgtcaacgcg ctcgggatcg ggccgatggg gcttggcggc gacgtgaccg cgctcgctgt
     1081 gaaggtgaag cgcgccgcct gccacaccgc ctccctcccg gtggcggtga acgtccagtg
     1141 ctgggcgtcc cgccgggcga gcgtggaggt gagactctga acctgaagac acccctcggc
     1201 gatgaggtgc tcgacctccg ggctggcgac cgggtcagcc tctcggggat catctacacc
     1261 gcgcgtgacg aggcgcacct ccggatgatg gaagagggta taccattcga cccggagggc
     1321 gccgcggtct accactgcgg acctgtggtc caggacggcc gccttctcgc tgcagggccc
     1381 accacctccg cccggatgaa ctcgctcagc gggtttctga tcgatgccgg tgtccgcgcc
     1441 ctgatcggca agggcgggat ggggcaggag gtggtggagc gtctccgcgg ccgcggcgtc
     1501 tacctcgcgt tcacgggggg atgcgccgcg ctcgccgcag cccgtatgcg gatcaccggc
     1561 gtttattttg aggaactcgg tatggccgag gcggtctggg tgctcgaggc cgattctctc
     1621 ccgctgacgg tcgggatcga cgcccacggc ggcgacctct tcgcggccgc acaagagaaa
     1681 gcgaaaacaa aatttcaaca gcgattcaat ataaagcagg ataccttctc atgaaacttg
     1741 tcatcgatga aacccgctgc aaagggtgca acctctgcgt gctggtctgc ccctacagga
     1801 tattcaagga agggacggaa ctcaacgacc gcgggatcgt aacaccggtc cttgaccggc
     1861 cggaacggtg cacaaactgc aggctgcagc ccctctacgg ccggctgctc tgcggcgtct
     1921 gccagatgat ctgccctgac caggcgattg cctgggtcga tgagaggccg tttgaaccgc
     1981 ataaggtgga ggtggagttt tgagcagaac cgagttcatg caggggaacg tagcctgcgc
     2041 cgagggggcg ctcgccgccg ggtgccggtt tttcgggggc tacccgatca cgccgtcgac
     2101 cgagatagcc gagacgatgg cgcgaaggct cccgaaggcc ggcggggtct tcatatcgat
     2161 ggaggacgaa ctcgggagca tcgccgcggt tatcggcgcc gcatggacgg gcgcccgggc
     2221 gatgacagcg acaagcggcc ccgggttctc gctgatgatg gagaacatag gttacgcggc
     2281 catgaccgag acgccatgcg tcatcgtcaa catccagcgc ggcggcccga gcaccgggca
     2341 acccacgcgg gcggcgcagg gcgacatgct ccagtgccgg ttcgggtcgc acggcgacta
     2401 cagcaccatc gctctctccc cctcctccgt ccaggagatg tttgacctga ccgcaaaggc
     2461 cttcaacctg gccgaccggt tcagggttcc caccttcctg atggccgatg agatcatcgg
     2521 ccacatgcgt gagcgtatga ccatccccga caaggtcgag atcgccggca gaccgcccct
     2581 ccccgagggc aggctcccgt tcgaggccgg ggaagacgga atccccgggt ttgcgccgtt
     2641 cggttcgggg agggcgatac atgtgaccgg gctcacccac gacgagcgcg gctacccgga
     2701 cacaaccgat cctgaggcgc acgaacggct tgtgaggcga ctggtcgcga aggtcgagtc
     2761 ggcgcgcagg gagatcgccg actacgaggt tgtgaacccg gatgcacgtg tggtctttgt
     2821 cacctacggc gccccctccc ggacggtgca gcaggtgatc cacgatcatc ccgatgaggg
     2881 tatcgggcac ctgcgcctcc gggttgtatg gccgttcccg gagtttgcgc tggctgagtt
     2941 tccagacgcc agggtctttt tgatgcctga actgaacatg ggccagatct cacgggaggt
     3001 cgagcgtcac gtcgggcagc cggtagtccc gatcccgaaa cttggcggtg acctccacac
     3061 acccgccgac ctgcttcgcg ccctggaggc gtatctatga gcagggccga gtggctccgg
     3121 gaggaccggt tgccgcacat ctactgcagc ggatgcggga acggaacggt catcaactgc
     3181 accctggccg cggtcgatga ggtggggtgg agacgcgatg agacggtctt catttccggg
     3241 atcggctgtt catcacgggc tccaggctac atcaacaccg actcgctcca taccacgcac
     3301 ggccgggcgc tcgcgtttgc caccggggtg aaactggcgc gacccgacct gaaagttgtc
     3361 gtcttcacag gggacggcga tcttgccgct atcggcggga accacttcat ccacgcctgc
     3421 cgccggaacg tggacatgac cgtggtctgc atgaacaacc agatctacgg gatgaccggc
     3481 ggtcagggga gcccgacgac gccgccggga gcgatctcga cgacaacccc atacggtgcg
     3541 agcgaaccgg cgttcgatct tgcggatctc gccatcgctg ccggcgcaaa ctatgtcgcc
     3601 cgctggacgt cctaccacgt cacggaactc acaaaagcca tcgccacggg cctccagacg
     3661 ccggggcttg cgttcatcga ggtgcggaca cagtgcccga cgaactacgg ccgctccaac
     3721 aggctgagag aggtttccga tatgatcgag tacctccgga gccacgcgat gctcgtccag
     3781 aagtatgagc ggttgatcgc cgagggtaaa ccgatcccgg aggggacgtt caccatcggg
     3841 gaactggtcc ggcggaaccg gccggcgatg ggggtgcagc catgaggcac gaggtcaggt
     3901 tctcgggata cggcggccag gggataatcc tctcctctgt catccttggg cgggcggcgg
     3961 cgctctacga cggcaagtac gccgtccaga cacaggtcta cggcccggaa gcccgtggcg
     4021 gggcgtcgat ggggcaggtg gttatcgatg acgaacctat cctctttccc gaggtgaccg
     4081 atcccgatat atacgtcatc atgtcccggc aggggtttga gaagtacggt gtctgcgcac
     4141 ggaaggacgc gttcatgctt atcgatgccg acctggtcag gtcgcgtccc ggttgccgct
     4201 gctgtgagat cccggcaacg tcggaggcga agaccaacct gggcagggag atcgtggcaa
     4261 acatcgttat gatcggcgcc cttgtggccg caaccggggt cgtgagcagg gaggcgatcg
     4321 agcgtgcggt gctcgacagc gtgccaaagg gcacggaggg cctgaaccag cgtgctttga
     4381 aacggggatt tgaacttgga gagcgagcgt gtaatccatg aaactgcttg aatacgaggc
     4441 aaaaagggtc ttctcggact acgggatacc cgttccgcag gggtttgtca tacgcgaacc
     4501 cggagagatc gcggcgcatc tctcggagtt cggggatgcc gtggtgctga aggcgcaggt
     4561 ggacgtcggc ggccggggga aggccggcgg cgtcctggtg gtcgattcgg cgtcggcggt
     4621 cgatagagcg cgtgaactat tctcccggga gatccggggc gtccctgtga gggagatcct
     4681 ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatgctg ccgatgcaaa
     4801 acttatcata gacgacaacg ccctcggccg ccaggggatc gaggtcaacc gcgacctctc
     4861 ggagcgcgag cgcgaggccg ggaggcacgg gttctcctac gtggagctcg acgggacgat
     4921 cggggtcatc gggaacggtg ccgggctaac catgtcgacg atcgacctga tcgactacta
     4981 cagaggccgc gcggcaaact tcctggacgt cggcggcggt gccgaccggg aacgggtgat
     5041 gcatgccgtc cggctggttg caggcctccc gtccgtgaag gttatccttg taaacctgct
     5101 cggcggcatc acccggtgcg atgaggtcgc gcacgggatc atcgccgccg acgtcggcgt
     5161 gcccgtgatc gtccgtatgg ccgggacgaa cgaggaggag ggccggcggc tgcttgcgga
     5221 gcacggctac aggatggtcg atagcatgga agcggcagtg agagcggcgc tggaggctgg
     5281 agaatgatct acggtgaccg aaacctgggt gtgatagtcc agaacatcac cgggaggcag
     5341 gggcggttcc acacggaact catgaacgca tacgcccgtg aggtaggctg gaaggggatt
     5401 gttgccgggg tcgcccccgg gaaggccggg atggaggtcg acggcgtccc ggtctacaac
     5461 accgtgaagg aggcgctcgc agagcacgat gccgccgcaa gcgtcatatt tgtcccggca
     5521 cccgccgccg cggatgccat catggaggcg gcatatgcag ggatagaact tgccgtcgtt
     5581 ataaccgagc tcatccccgt ccatgacacg atgaaggcga tcgcctacgc gaaactccac
     5641 gactgtgcgg tcatcggccc gaactgcccc ggtctcctat cgccggggga gtgcaagctc
     5701 ggaataatgc cggcgcacct tgccacccgc ggagaggtcg gagtggtctc gcggagcggc
     5761 accctgacct acgaggtggt cgatgaactc agccgtgccg gtataggcca gagcacggtg
     5821 gtcgggatag gcggcgaccc ggtgataggg cagacgtttg tggacgtgct tgcgcggttt
     5881 gaggaggacg cgcatacaaa ggccgtcgtt ctcatcggcg aggttggcgg gaaccttgag
     5941 gaggagggtg cgcggtcgac cgatctgccg gttgtcgcct acatcgcagg agtgagcgcc
     6001 ccgccagaga agcggatggg tcatgccggc gcgatcatag agggcggaga gggcgatgcg
     6061 cggtcaaagg tcaggcggct ctctgagatg ggggttcctg tcgcggccag accctcggag
     6121 ataccggggc tgatcaggga ggtgttatga acggcgaact gatgcttgca accgcctgtg
     6181 aggttgcaaa gaagatcggg gcaagggcgg ttgtctcatt catcgaaccg gccccggtca
     6241 tggcagagcc gcccgggatc ccgatcataa gggtgcagga acttcaactc gatgtcctca
     6301 aagacctcac gatgcacgat atactggagg tgagtcagcg ccacctgctc gacgccgcgg
     6361 ttcacctata cctccagcgc aactttgaga gtgggcttat cgtcggggtc ttctcatacg
     6421 ccatcgtcat atacgatatc gaggagggga agaacttcat aagcctcaag gacttctccg
     6481 atatcgtccc ccgtgaggtg ctccacgccg tcctcactct ggccctcgag atagcggtcg
     6541 aggggaggga ggggcgcgcc ataggcacgg cgttcatcat cggtgacccc gatgagatct
     6601 tccgccactc gcaccaggcg atcctgaacc cctaccacgg ccaccccccc gccctcaggg
     6661 acataaagaa acgggacaac tgggagagcg tcaaagagtt cgcccagctc gacggcgtct
     6721 ttgttataga caaaaacggc gaagtccggg cggccggacg ctacctggac gtcagcggcc
     6781 gcggaatatc cctcccgagc ggtcttggcg gccggcaccg cgcgaccgcg tccatcacac
     6841 gggagatccc ggccatcggc atcacggtct ctgagagcgg gggcgtggtg cgcctcttca
     6901 gggaaggcga atgcaagctc agtatccgtt cagacatccg cctcaggtga gtgcgttaga
     6961 aacggttata tgaattgcaa tcatttctct tatcacactt tagaactacg gtggatttga
     7021 tgaccaggaa catcagcgta gaggggcttg agcagatccc gatcgagaag cagcggatag
     7081 aactggtgga gcgcaagtgc ctggggcacc cggacagcct ggcggacggt atcgcggagt
     7141 cgatcagccg cgcactcagc aggtcttaca tggaggagtg cgggagcgtc ctgcaccaca
     7201 acacagacca gggagaggtc gttgcgggcg agtcgctgcc gaagttcggc ggcggcatca
     7261 ttacaagacc gatctacttc ctcatatccg ggcgggcgac aaagaccttc aacggcgtga
     7321 acatcccggc ggacgcgatc gccgtcgagg cggcacggga ctacataaga aagacactcc
     7381 ccatgctcaa catggagcgc gacgttatcg ttgactgccg gatgggcaga ggttcgacag
     7441 accttcagga cgtcttccgg tcgtgctcgg ggaaggcccc gcgggcaaac gatacctcgt
     7501 ttggtgtcgg gcacgcgccg ttcagcgagg cggagagcat agttcttggc atttctgagt
     7561 ttatggacag gacgctccgc ccgaaacacc ctgtcatcgg ccaggactgc aagatcatgt
     7621 gccttcgtga cggtgataca atcaacctca cccttgcgat ggcgtttgtg gaccgctact
     7681 gttcgggcat cgccgactac atcgagcaga agaacttcct gacggagcag atagcggcgg
     7741 ttgcagggca gttcaccagc aggagggtca acgttgccat caacaccgct gatgacctgg
     7801 ataactgcag cgttttcctg acggtctcgg gcacctcggc cgagatgggc gatgatggtt
     7861 cggtcggccg gggtaaccgc gcgaacgggc tgatcacgcc gaaccgcccg atgagcatgg
     7921 aagcgacaag cggaaagaac ccgatcaacc acattggaaa gatctacaac ctcctggcaa
     7981 cagagatcgc gaaagactgt gttgcaaggg tcgatggtat agaggagatc tacgtccgca
     8041 tcctctcaca gatcggccac ccgatcgacc agccgcatgt ggcaagcgcc cagatcctcc
     8101 cgaaaagcgg ttttgaggtg agtgctctca ggccggaggt cgaggggatc atcgatgact
     8161 ggctggagag gatcaccacc atcaccgaga aggttatcag gggagaactc tctacttttt
     8221 aaccatcccc tgtaactttc tggagtctgc aatccatgag caaaccagta cccaggagcc
     8281 cggatccggg gtttatccgt cttgccatcc cgaacaaagg ccggatcgcc ggtccggtcc
     8341 acgagttgat cgagaagagc gggcttcacc tggccgacgg cggcggtgag cgcaggctca
     8401 tcgcccggac gcgcgacccc catgttgaga tcctctttgc ccggccgatc gatataccgg
     8461 agtacgtggc aacaggtgtt gccgaccttg gaattaccgg gagggatatg gtcctggagc
     8521 ggggttcgga ggtcaaggag ttgctcgacc tccagaccgg cagggcgacg ctggttgtgg
     8581 ctgtgccgga ggagtctgat gtcgagagca tcaccgatct cgatggggcg agggtggcga
     8641 ccgagtttcc agggataacc cgcgctttct ttgcggagca cggggttgcg gtgacggtgg
     8701 tgacggtggg cggtgcatgc gaggcgacgc cgcacctcgg gatcgccgat gccatagtcg
     8761 atctcacctc atcggggacg acgctccgga caaaccatct ccgcgttgtt caggagatcc
     8821 tgacctcaac cacgatcctt gtggcaaacc cgagatcgct aaagacccgg ggcgagaaga
     8881 tcgatgaggt tgtcctggcg cttgacagcg tcctccaggc aaaggggcag tgttacctga
     8941 tgatgaacgt ccacaggagc gctcttgcgg acgtgaggga gatcctgccc ggcctctcgg
     9001 ggccgacggt gatggatgtg gcatctgatg agaatctggt tgccgttcac gctgttgtaa
     9061 acgaggaacg ggtctatcaa ctgatcaacc agttaaaacg cgccggcgca aaagacatat
     9121 tagtcatgcc catcgaacgg atcatacgtt gagagcatgg agatctatcc tgcagtcgat
     9181 atcctggagg gtcggtgcgt gcagcttgtg caggggcgcc gggacgcggc tacggtttac
     9241 ggcgacccgc tcgcctgggc gcgccggtgg ctcgatctgg gagcggacgg tctgcacgtt
     9301 gtcaacctgg acggggcgtt cgggaaggca aagaggaacg ccgacctgat ccgccggctc
     9361 gccggcgaga cggagacgtt tatcgaactc ggcgggggta tacggagcgt ggaagacgcc
     9421 gcggggtggc ttgatgccgg tgtcgaccgt gtgatagtct cgacccttgc cgtcagggag
     9481 ccggaggttg tccgtaggct tgccgatgag ttcgggagcg agcgtgtgat ggtcgggatc
     9541 gatgctcggg gcggcgaggt ggtgatcgag ggctgggagc gcccggcggg taattacctc
     9601 tcctgggcgg aacggtttga gtctctcggc gcggggtctc tcctctacac gaatgtggat
     9661 gtggagggtc tccagcaggg gatcgatata gccccaatag aggctctcct tgcccgggtg
     9721 agcgtcccgg tggtggtctc cgggggggta tcgagcgcca gggacgtctg cgccctccgg
     9781 gcggcggggg cggcaggggc ggtcctgggc tcggctctct actccggcag gatccggctc
     9841 tctgaggcta tggaggcggc gcatgcgaag aactgaaatc caccgcaaaa cacgggagac
     9901 cgatatcagg ctctccctcg acattgacgg caccgggacg gcgagaatcg agacggggat
     9961 acccttcttt gaccacatgc tggagtcgtt tgcccggcac ggccggttcg atctcggtat
    10021 cgaggctgcg ggtgaccttg cggtggatgc gcaccacaca atcgaggata tcggtatagc
    10081 cctgggcaaa gccctggctg cggcggtcgg tgaggggaag gggataacac ggtttgccga
    10141 cgctgccgtg ccgatggacg aggctcttgc acgggtggcg ctcgatcttg gtggacgggg
    10201 ctacctggtc tttgaggggg gtttctcgcc tgcagggccg ggcggtatac cgggcgacct
    10261 catcgagcac ttcttctaca gtctctgcac aaacgcggga ttaaccgcgc acatcagcct
    10321 tacgggcagg agcgatcacc atgtatgcga ggcggcgttc aaggcgtttg ggcgtgcact
    10381 ccgggcggcg gtggccatcg atccggctat gggcggcgag gtgccgagca cgaaggggac
    10441 gttctgagac ccgccgggta agccgcgtat cggctcataa ataagcatct ccttgatctt
    10501 ccggggaaac actgggcggg ggttgcaacc cggctgagat tgcggatgtg ggcgtctgag
    10561 actatggggc gtcatctccc gggatatatg ccctttgcag gacacccccc atccgggatg
    10621 gctactccag aatcgggcca gaaaagcgct gccgccatgt aactgaccga ccgcgcaatc
    10681 gaagttttga aaccggaacc agtcacacct cctgccagcg gtcagcgctc gggctgacgg
    10741 ttcggatcgg agaatggggc ccatatggac cccgataaag agaaagggaa aactgccggc
    10801 ttacgcggac ttgaccgcga gttcgacgtc ttctgccttg atcgtcttgc gtccagcgtg
    10861 ctgggccagc ctgttggctt cccgggtcag ttcagcgatg taggcctctg ctttggcaac
    10921 aagtgcagca gcagcatcgc tgccaaccct ctcagcacca ttcttctttg cgatacgtac
    10981 aaccgcagca atgggtagat ctgccatagt cttactccac cgttaaagag gtgtatcagt
    11041 taatatttaa acatttcggg ataatctcag agatccgggt tcctctttca tttctaaggt
    11101 gcatccgaag agggtatccc gataagccag atagaatcga gccgattaaa aaaaccagaa
    11161 taaacccctt gatccaggat tagagaggcc aatcagggtt tttccgggag caccagcgat
    11221 ttcgcaagca ttatggcatt ggtgtaatct gctgaggcgc gcgaggtatc cacgaatatt
    11281 ctatcgatat ttaccaccgg ggcaccatga cccctcccgc cgccaaggag agcgagtgta
    11341 cgaaagatca ggttcccggt tataccgtcc ggggcgatca ccaggccgta ccgccccggc
    11401 acctcctcga ccagtatctc aatatgctct gcaccgctca ggcgggcgac gagttcggca
    11461 tccgccatcg tccggtcgac tgcggggtga cgcccaacat caccaagcct tccaccagag
    11521 aggatcgcca caccctccgg cagcccgaac cgcctggcga tatccctgcc ggcactggca
    11581 aaccggacct tctgctcgac agtccagccc tcatcgaccc cgacaggggc caggaggaag
    11641 agatggccgt ccggcgtctc caggagggcg atccgctcca ggcggtcgac gcccgcagcc
    11701 cgcttgaggg ccttcagggt ggtggatgcg ggaagcgtcc ccctgaccgc ggcatcgatc
    11761 ctgccggtgt agagagcctc gacgatggcg gcctccggct ccccggcctc gatgaccgtg
    11821 acctcatcac caaacgagtc ggcggtcccg ggcggcgcga ataaaaccag gttgacctct
    11881 cctctgacag ccctggcagc cgagaggatc ttcgcctccg cttttgccgc acccagccct
    11941 atttttatcg gcatcccccc ccgcctgcaa agatccgcat gatgccgtcg cggtccaggt
    12001 caaagacctg cgtctccctg cccaggtagc ggacggtcaa acgccgatcg ccgttcacct
    12061 ccccgatggc ggctgccgcc ataccgacgt cggcaaacct gcggcagacc tcatccacgt
    12121 tctccggttt gaccgtgagg atgaatccca tcccggggta catccgcacc cagtgctcga
    12181 atgagatgcc gagtgcggcg aggtcgggcc tcggtataga ctcaaggttg accaccgcgc
    12241 ccttcccgct cacctcaaga agcatcccga gcgtcccgat gacgccgggg ttgctgatat
    12301 ccttcccggc cgtcaccagg tgctccctgc ccaggtcctc catcaccagg atctgcgccc
    12361 gcacaaccgc cgcggacttc atcgtgaccg aatcccagtt gaagcagcag gaggggtgaa
    12421 cccggccgtc caggtctatg gcggcgacga cgctgtctcc ctcttctgct gtatgggaga
    12481 agatgatctc actcatcctg gccgtcccca gtatagccac atccacgacg ctgtaggggg
    12541 tatccgggtg cagatgcccg ccaacgatgg gaacgccgaa ctgcgaagac gcagtgacca
    12601 tgccccgcac cacctcgtcg cggatctggt cgttcaccgc ggagaggata tcgaccatcg
    12661 ccaccggcct gccccccatc gcagcgatat cgtggatgtt cacgagcact gcacagtaac
    12721 ctgcccagaa ggggtccgcc tccatcagcc tgctccagat accgtctgct gcaagcagca
    12781 gcgcatcctc cgcattgtgc cggatgaccg cggcgtcctc accgaacgat gcgacaacat
    12841 ctgggctgtc cacccggaga gcccggatca tatcgcctat ctcccttttc cgggtgacac
    12901 cctcgtattc cctgacggcc tgtgctaccg tatcggtgga gcaatccttc accacaagaa
    12961 caccaacatc tccggtttct tatggtattc tgcctcataa gagataatct atttcatctg
    13021 cagcattata catcccgttc cagactgaac cgatcctggc tggagatggg gggagacacc
    13081 cccgggagcg gacattcgcg ctccagtgca gcctcaccac ctcattgcaa ccagggtggc
    13141 atttttgatg caccacaacc caatagattg acatggactg ggcggagaag tacaggccgc
    13201 agcacctcaa tgaggtcgtg gggaactcaa gcgccatccg gcagatgtat gagtgggcgc
    13261 gggactggtc gccggagaag aaaccgctta tcctatacgg aaaacccggg accggcaaga
    13321 cctcaagcgc ctacgccctt gcaaacgaca tgaactggga ggtggtggaa ctgaacgcct
    13381 ccgaccagcg tacgaaagcc gtccttgaac ggatagcggg ctcgagttcc accacggcca
    13441 gcctcaccgg tgcgagcagg aaactcatcc tgctcgacga ggccgacaac ctccacggcc
    13501 aggccgacag gggcggcgca aaggcgatca tcgatatcat cgcggcgtcg cagcagccta
    13561 taatcctgat agcaaacgac tactacgccc tcccgaaaga actcaaggcg gtcacggaac
    13621 ctgtccagtt cagggcgctc cagagccgct cgatcgtccc ccgcctccgc cagatctgcg
    13681 ccgccgaggg ggtggagtgc gaccccgccg cactcgagga gatcgcaaac cgcgccgacg
    13741 gagatatgcg ggcggcggtg aatatgctct acgctgcagc gatagggaag gatcgtctcc
    13801 tcgcaggtga tatccacacc tcaacaaagg acgagcgctc aaccatattc gagctcgtcg
    13861 gcgcggtctt tgccggccgg aaggacgccg acctcctccg gctggtgatg gaggttgagg
    13921 atacccccga caccgttgag cagtggctgg aggggaacct cgaccatata cccgatctgc
    13981 cctccagggc gaggggctac gcttatctcg cgagggcgga tgagtatatc ggccgcacct
    14041 accagcggca gtactacacc ctctggcgct acgcaaactc ggtcatgctc cttggcgtag
    14101 ctgaggctgc gggggggcgc ggcgttcgcg gccgcatcat gccgccgtca cgctggcaga
    14161 agatgggcgc atcaaagaag cagaaggccg tccgcgccgg tctgtaccgg agattgagcg
    14221 gaatcctgaa gatcccggaa gaggcgctgc gggagagttt tttcacggcg atcagcatac
    14281 ttgtcgagag ggacgccgca agatacgtcc gcgagttcgg gctcgatgcc gacgagttga
    14341 acctcttcat aaacgataag ccgcgggctg ccagggtggt cagggaggcg gcaaaggagg
    14401 agaaggaggc aaagaagaca cctcccgcaa agaagggagg cggcagggcg aagaaacccg
    14461 cagaagacgc accgaaggac gaacccggtg aggcgaggcg aggccctcca ccgggccagg
    14521 cgacgctctt ttaaggccgg cggcggtggt agaggtggag ttgcacgccg ccagcgtcca
    14581 cgacctcgcc gccgtcccga tatacctcgc accccaggtg gtagaccagg aggtctgagt
    14641 ttgtccgggt ggcaagcgat atcagggggg gaaccatctc cacgccgggc ctcacagagt
    14701 agatcacgtc tgcgcccagg tagagcctca tatcgggttc aaatatgtcg tcggtcacca
    14761 cggcgagccc ttcgtgctgt atgccaggcc tgatgtcggt gcagagcata agggcgccgg
    14821 cagccgcaac cacccccgcg gccacggggt tattcccgat cccaacttcg accgcccggc
    14881 ggtagcggct ggcgatgtac tcccctatac tccgttcaat atgtttatac tggcccatca
    14941 cgaagagatt aggtaatggg catccatatt ctttgggaca cagaagcacc ggtgggtatc
    15001 gagctcccgg tcatccgtat gatcgcgatg atccttggag aagagtcgga acttgtcgaa
    15061 tacccgctcc tcatcgatgg ttatgacagg gaacgcaacc agcacgatgc tcataagatc
    15121 ctggaccgcc tgcaggatac cctgacgcgc aggtatgata tcgatggtcc gctgctgttt
    15181 gtaacctcac gcgacctcta tatcgggggg tgcgactttg tcttcgggct tgcccgcccg
    15241 agagccggtg tcgccgtcgt ctcgacagcc cggctgggga acgactatta cggcagggtg
    15301 ccggacgata ccgatctcat agacaggaca gctaaggagg gggcgcacga actcggccac
    15361 ctcctggggc tcggccactg ttcaaacccc gaatgtgtca tgttccggcc gcagactctt
    15421 gacgaactcg acaggaagaa gaaggtgctc tgcccggtct gcagagaggc tctggcacca
    15481 ctacaggaag gatgagatgg aagcggcctc actctacctg gccaaggtct tctgagaggt
    15541 cttctgtgag gtctaccgcc agaaaatcac tgctggatgg gagcacaacg ctgaagttct
    15601 ttgtggtgtc gacacggacg gtgccttctg cgagggtcat atcgaggatg tgaccgccgg
    15661 cggtgcggtc agtggtgatg aaatgcaggt ggtaaccggg gacgttgaca ccttttgcca
    15721 gtcctggtga ccagaacccg acggcggtcc cggtgatatt cttgaaggaa aagaccgtct
    15781 ggtttgctgt caccagcgcg agacgtgggt agggtttttc ctgggcggga acgctcctgg
    15841 tcaccacctg cgagaaggtg ccatccaccc tgatcgcata gaagtagttc tcagagggga
    15901 actcatcccg cacgcggttc tccagatcag gaagggtcat cggcttctcg accgcgatct
    15961 cgatatcggg gtcaaagaag gtcgtggccg caaaaggcgt tgtcgcgttt ccagcgaccg
    16021 ggtaagcccg accatcgacc ctgatcaggt accacccgcc gtcgagaccg atcagttccc
    16081 cgtcaagccg gtcgccgcac ccgatcccca ggtcgccgtg cgacgcgagt tcgtcaaaac
    16141 tcatgctgcc atcgtatatg ccctggagaa gcgcatcgat ggtcgagacc tgaaaaaggg
    16201 tctcccggtc agtttctgcc gttgactgga gtggaacggc cgcaaacccg atacaggcgc
    16261 ctagagtgat taagatggca gcaacgatga tgaaacgcag cagtctgccg ttggtcatgc
    16321 gctcctcagg ttcctcctat atcttcatac cagatgtctg gatgctccct tatgaacgcg
    16381 gccatcatct ccttgcagac ctccaggtca agatcgatta cctcgacagc gtgcgactcc
    16441 aggaactccc gtgctcctgc gaagttcgca gactcgcccg ccacaacctt cctgatcccg
    16501 aactggacga ccgccccggc acagaggtag cagggcataa gggtagagta aagtgtgcat
    16561 tctctgtagt tccggatcct gccggcgttt atcaggcagt cgatctcggc gtggaggatg
    16621 gggttgttct cctggacacg gcggttgcgc cccctgctga tgatctggcc gttacggacc
    16681 aggacggagc cgatcggtat tccgccttcc cgaagccccg cttccgcctc ttcgatggag
    16741 cacttcatga agaggtccat acaacacccc ccagggcgcc ggagccagcg tatgacaact
    16801 caaaaacacc tgatgatcta taaagatgct acgggtttat aaacgcatat gaaacgaaaa
    16861 cgcccggttc gggcgtgatg cgctggaggc cgacctcttc aggcggttga ggcctctcct
    16921 ctgattccta tctcggcgag cgcctgccgc gcggcccccc tgacatcgcg gtcgatgtcg
    16981 tccaggagac gaaggagggc gtccctggcc agggggttgc cgatacggcc gagcgccagg
    17041 gccgcctccc tcctgaccct gggtttttcg tcgccgagca gccggatcaa cggatcaacc
    17101 gcacgagggt cgccgatctg gccgagagac ctggccgccg ccatccgcac ccaggaactc
    17161 tcatcctcca ggcagtgtat cagcggggtg accgcctccc ggccgtccag gatcccgagc
    17221 gcagtcgccg ctctccaccg gacatcggcg tagtcgtcgt ccagcgcctc gatgagcggc
    17281 tccacggcgc gatcgtcagc gaattccgca agcgcatcag cggcccgcca gcgttcgttg
    17341 agccctcccc acctcagcat cgagatgtgg cggttgaacg ccctctcgac gctcttctca
    17401 ccggtctcct cctcttccgg cacaggcaga tccacctcac gctcatctgg gggtcatccg
    17461 ggtataagtt ttttggactg cgggccggcg agagcccggg agggcatgaa gccagttttc
    17521 ttcatggctg tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcacc
    17641 ccgcgggggt gcatttttct cttctccgca ccctctccat atgtatggac agtccaagga
    17701 cggttaaacc agaggccacg gcgtttcccc cggaactgga gcgcctcggc atcgctgccg
    17761 gagcaaggat cgacataaga gacctcgatg cactctcaaa gcaccacaac ttccaggctt
    17821 acctctactt tgaggaggac cttgccaggg gctccacgct tggcagggac ctggaagact
    17881 accgccgcgt tccggaagtg gagcgtccgt tcgtcaacct tgatgagttc ttgcggttcg
    17941 ccaccgcatc agacccgctc ttcagcagtt atctggatga actccctctg gtgatcgaga
    18001 tcctggcctg cggggagatt catgcagagg atggggaggc cgtggcctac gtcaaagggc
    18061 tgatgccttt ccttgacgaa ctcgatgtgg atgatgcact tcccggtgca tcctgagaaa
    18121 ctcaatcaaa acttattttc ccgtgatgtg agtcctcgac tgagtgggag ggctctactt
    18181 tccgcatctg ccccccccgg caccggtctc tctggtctcg tcgcctccac cggggtggac
    18241 gggacggcca gacctgaaaa cggcgccctc ttcattcgag cctgacggtg aagacgcctt
    18301 tgaggatatc caggctcacg tcaaagttcc tgacggtgtg ggtgagcgcc cccatgctga
    18361 tgatatcgac cccggttgcg gcatacacag cgaggtcggc gccgtcaacg cctcccgaga
    18421 cctcaagggt cacccgctcc cgcagcccct cccgtttaag tgcctcgacc gccgctttaa
    18481 cgtcctccgg cgtcatgttg tcgagcatga tgacccgggc gccggcctcc gccgccgtga
    18541 cagcgtcttc cacagtctcg acctcaacct cgatcatcag gtagcggctc cgcgcctttg
    18601 cccggcgtat cgcttccgga agcggcacca gcgccaggtg gttgtccttg atcaggatca
    18661 tatccgagag cgtgcagcgg tgtgggtcgc caccgccaag gatcaccgcc ttcttgtcga
    18721 gcacccggag ccccggcgca gtcttgcggg tcgcggcaac ccgcaccccc ttagagacac
    18781 ggctgaccct ctcaaccgcc tcacgggtcg ctgtcgcaat cccgctcatc ctgccgatga
    18841 tgttgagtgc cgtccgctcc agaaggagga tcgccctggc aggcccctcc aggtccatga
    18901 tggcggttcc cgcggcaacc tcgcggccgt cagcggtatg gaggtagacc gtgaccccga
    18961 agtgctcaaa gagcgcccgc gcctctttaa gcccggcgat gaccccggaa cctttcgccc
    19021 ggatcaccgc ccggcagaca acgtccggaa cgaccgtctc ggaggtgaga tccccccagg
    19081 gcgcgtcctc ccggacgaac cggagcaggt cgtctatcgg tatcacattc gtcacacccc
    19141 ggcagcgagc atccgctcga tggcccgccg tgcgtcctcc atcaccccgg gaggaaggac
    19201 gatctcgtgc tcctcgcggt cgagcgcgcg gaggagatcg gcaggcttta tcttcttcat
    19261 atcgctgcat acggcgtccg gcttctcgta aaagaccctt ccgggataga gataccggag
    19321 acgggagacc atctcccgct ccgtgaagac ccaccagggc ccgttcccgc cgtctgctgc
    19381 gcccctgacc atccctcccg tggatgctat catgtcggcc tcttcctgga cctcaggcag
    19441 acattccggg tgacagacga tgatcccacc cctctttcgc gccgcctcca catccgccag
    19501 agaaaacccg gtatggacgt aacagtgccc ccccggcggg agggggatga tcctcttatc
    19561 ggggagttcg cgctggacgt atgccgcaag gttggcgtca ggtccaaaga gtatctcgtc
    19621 gttctggagg gaggcaacca cccggaccgc gtttgccgat gtgcacgtta tatcggcgag
    19681 cgccttgcac tccgcgctgc tgttgacgta gaccaccacc gccgcacccg ggtgccgccg
    19741 gcgtgcctcc aggatcatcg ccggggtcag gtggtcagca agcgggcaac cggcatcctc
    19801 caccgggatg atcaccttcc ggccggggtt caggatcttt gccgtctcag ccatgaaccg
    19861 gacaccgcag acgacgatca ggtcagcccc cgtctctttt gccttcaccg caagctccag
    19921 gctgtcgccg acaacgtcgg caagcgcctg tatctccatg ggctggtagt tatgcgccag
    19981 gatgaccgcg ttcttcctgg atttgagcgc acggatctcg tcttccatga gttacgttcc
    20041 caccaccagt ggattctcaa gattccgcag gagcgccagg atcgagaggg ctgccatata
    20101 actcgtcgca gggttatcgg ggctcgggac gttgctgacc cggatgtaga tatccccgaa
    20161 ctccccctca acgaagacct cgtgtatgtt cctctccacc gccgggtcga cccagagctc
    20221 cacctcagcg tccctgccgg cggcaagtcc cagcgccacc gcgacgttga tgttttttgg
    20281 aaactgtcgt atacaatcgt gcgccagtcc cttaaatagt tccgtcctgg ccgcaacatc
    20341 catcccgagc gacgccgggg gtttggtcgt ccggaggagg agttgccgcg gcggcgaaac
    20401 ctggcctatc ttgaggttat cgagccccag gatcgcgccg ctcgggatcc ggatcttctt
    20461 tccctcctcc cgtgccaccc cgacgagatg ttcgcgaaaa tcctcctcag ccagggcgcc
    20521 aacggagagg acgacgatat cccggccaga gcggaggatc gcctcgccga aggttctgac
    20581 agcgttgatc gaggcggctt caacgacgat cgagaactca tcgtgcatga aggcctcaaa
    20641 atcggtgtat gcgcgagcgc cgcagagcgc cgccagttct tccgcccgtt ccgggatgat
    20701 atcatagacg gcggtgatct ggatgccccc ggcgtgtgcg gcgatgatat gcccgacgtt
    20761 gccgcagccg agcaacccta tttttatcat cgaataaaga agttgtaatg tcagcggtta
    20821 agggttgtgc ccggcacgaa accggcaggc ggtggctaaa accgatccat ctgcatcaca
    20881 ccacccgggt ttctggccgg gcgccgaccg cgcagaaagg agtgttgagc agccatcacg
    20941 cttgatgagt tacggggaag acgggtctac atcgagacct acggctgcac ctacaaccag
    21001 gccgatagcc ggaggctgga gcggatactg gaggggcagg gctgcaccat aacagggcgt
    21061 ccggaggagg cggacgccgt tgtcatcaac acctgcacgg tgatcgggag aaccgagcga
    21121 aagatgctcc ggcggcttga ggagttcgcc gaccgcgatc tctatgtgac cgggtgtatg
    21181 cccctcgttc agatggatga gatccggtcg gtctgcaatc cccgtgtcat ccacccggac
    21241 gagatccagg agcgttccgg gagcatcggc acccggggcc cgggggcgac cggcgtcgtc
    21301 caggtggcat ccggctgcgt cggccggtgc agttactgca taacccggct tgcacgcggg
    21361 cggctgcgca gcgcgccggc tgaggcagtc cttgatgccg tcaggggtct tctcgcctca
    21421 ggagcatacg agatccaggt taccgggcag gacgttgccg cctggggcct cgaccgcggc
    21481 gaatcgttcc cggacctcct gcgcgggata tcgggcatcc tgggacgatt tgccatccgg
    21541 gtggggatga tgcaccccgc ctcggtcacc ggtatcctgg acgatctggt cgaggcgttc
    21601 cacagcgaga aggtcttccg gtttctccac ctccccgttc agtcaggttc ggacaccgtt
    21661 cttgagcgga tgcagcgtgg ctacaccgcg gcggacgtaa tccggatcgt ggatgcgttc
    21721 cgggaggagt tcccggatat gatgatctct tctgacttca taaccgggtt tccgggcgag
    21781 acggctgagg agttccagga gacgctcgaa ctcctccgcc ggtgtgagtt tgtgaaggtc
    21841 aatgtcaccc gctactcccg gcggcccggc acccccgctg ccgcattgaa agaccttccg
    21901 gataggctga ggaaggagcg gtcacgggcg ctgcttgttg aggcgaaccg gatctacgac
    21961 cgctacaacg agcgctggat ggggcgggtg acgccggtgg tcgcgacaga gaagaacgtg
    22021 ccgggctcga ccgtctgccg taacccctgt tacctcaacg tcgtcatcag agacgacctg
    22081 ccgccggggt tctctgggag agcgctcata accgggaacc accgccacta cgtcatcggg
    22141 gaactggttt gaggccgc
//
LOCUS       DAOSZX010000015        21747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1968_length_21747_cov_21.215840, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000015 DAOSZX010000000
VERSION     DAOSZX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21747
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_21747_cov_21.215840"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /gene="polC"
                     /locus_tag="PK196_02470"
     CDS             complement(<1..2200)
                     /gene="polC"
                     /locus_tag="PK196_02470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HON40322.1"
                     /translation="MEVSPAIARYFEEIDAGLEAAMRLAAAARARGLDPRTEVEIPVA
                     SDLADRVEALLGYRGIAARIRELEQEMSREEAALRIGDDFVARKFGETTREDILDHAI
                     RAAMALLTEGVVAAPTEGIAKVGLGRNDDGTDYLKIYYAGPIRSAGGTAQALSVLVGD
                     YVRRALGIGRYIPRPDEVERYIEEIRQYNNIMSLQYLPSEKELRMIIENCPVCIDGEP
                     TEQQEVSGYRNLERVETNTVRGGMALVVAEGLALKAPKVLKNVRKMKMEGWDWLDDLI
                     SGGTTANSEEDDAGAAIKPRDKYIRDLIGGRPVFAYPMRRGGFRLRLGRARNTGFAAA
                     GLNPATMHILGDFLAVGTQMKIERPGKAAGIVPVDSIQGPTVKLQNGEVRRVDDAAEA
                     RRIAGQVEEILDVGEILISFGEFMENNHPLIPPAYCEEWWMLEGGPRHPENELEAIEF
                     AIEGAPLHPDYTYMWDDITPADIARLADAVSTGGRIEDGVLLLQNSPEVKAILEELLV
                     PHHLSGDRIAIREHLVLLACLGLTLDLEKRPEWQDAPMEDSLDLVMHLSGFRIRSRAG
                     TRIGGRMGRPGKSKPREMRPPPHSLFPIGDEGGSRRSFQAACISKPRSNTDGGVIEVE
                     AGERRCPACGAFTYKNLCDCGTHTNPVFRCPKCGKEVGEGVCPRCRVETVCLQKVTIN
                     VRAEYAAALERLDLRESSIALLKGVRGLISRERPVEPIEKGILRALQNLYV"
     gene            complement(2282..2794)
                     /locus_tag="PK196_02475"
     CDS             complement(2282..2794)
                     /locus_tag="PK196_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON40323.1"
                     /translation="MTERIRIEELIADLRSGTLEVRRAATAKLGASGKEAVEPLIAVM
                     QTGDSDLRWYAANALVLIGSPAIDPLLTAMRGTDEIDLRRYAAAALAEIGEPAIEPLI
                     DIIARAERDLMPFAVMAICRIGEPAVEPLLRLMESPDPDTQERAALILWRMGEPGAGP
                     LAEALKVKDR"
     gene            complement(2787..3989)
                     /locus_tag="PK196_02480"
     CDS             complement(2787..3989)
                     /locus_tag="PK196_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON40324.1"
                     /translation="MARPAVIVLVAALAVLFDLAFGTVAYTHFFYLVLILAAFWYRRR
                     AVAVGLLLAAVHVTIEYLFHGPPDASILVSAATFIIAAWLLGYLFEVAGRRKGELHFR
                     IGEPKPAACERDTGRLIALLSNRDPGTRYRAAGCLGDRGDPAAVEPLAALLTDPDPGV
                     RWKAAEALGKLGPAAVGALTEGLRHEDADVRWMAAVALGEIGDPAAVPGLLAALDDPD
                     TYVRSRAALALAEIGEAALGDLIAAIHNGDERVRWGAAIALGKIGGERAVEALIKTLS
                     DPDEDVRLRAAAALADIGEPAVPRLMDALGTDDDRLRQGATAALSQIGRPAVPAVVRA
                     LREAGDWRIRAGAARVLGRIDDRQAVEALISGLDDAREEVRSAAREALLAASGRHNSR
                     EEAPERYD"
     gene            4272..6950
                     /locus_tag="PK196_02485"
     CDS             4272..6950
                     /locus_tag="PK196_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HON40325.1"
                     /translation="MPDYHALSVDDVQQNLGTGRSGLSTAEAEERLRRYGKNILREEA
                     RETRLQVFLRQFKSILIIILIVAAAVSFAVGEPLDAAAILIIVFLNAILGFTQEWQAG
                     EAIAALKKMLVQRAVVIRDGERREIDAPEIVPGDLVVVEMGERVPADIYLIEATSLQV
                     DEAPLTGESAPVDKAPGSLPPETPLAERSNMIFAGTTVVNGHGRGYVVATGMQTEFGL
                     IAGLSQQVEDETIPLARQMDRLGRDLGLIALGIAAIAVVIGLLQQRELLEMFLVGVSL
                     AVAVIPEGLPAVVTLTLALGVKNMMQRNCLIRHLPASETLGAVSVICTDKTGTLTRNE
                     MTVVRVWTPEAEVDVTGAGYIPEGEFILDGAPIDPLADPGLRRFLQTVLICNHASLGH
                     GEEGWRIFGTPTEGALVVAAAKAGLSREDLPEPVKEFSFNSTRKRMTVICPGREGGGY
                     IACVKGAPEVLLERSTRMLRGGSAVPLTDGLRREVLLAIEGYASQGLRVLGAACRPLS
                     TAADWTPEAVEDDLIFLGFAGIVDPPRPEAAEAISLCRSAGIDVIMITGDNPLTASAI
                     ARVLGLSSEGALTGADIESLSDDELAERLRTTNVLSRVTAEHKLRVVDLLSRDRKVIA
                     MTGDGVNDAPALKKASIGIAMGIKGTDAARESSDMVLVDDNFASIVAGVEEGRREYDN
                     IARFTRYLLSSNVGEIVAIVGGLLLGLPLILIPVQILWINLVTDGFTALALGLEPAER
                     DVMQRRPRDPEEPILNRNAYLVILLLGAWLGLLTLHAFLGVYEVDLDRARTMAFTGLV
                     IFEVYNVLNFRSFRFPLYRIGFFSNPALILAILGTLALQAVVVYVPLMQIFLGTAPLT
                     LADWGLLAILGLPVLIAGEVYKVLRLRGGRPSRAAG"
     gene            complement(6984..8144)
                     /locus_tag="PK196_02490"
     CDS             complement(6984..8144)
                     /locus_tag="PK196_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HON40326.1"
                     /translation="MYLDNDDERVLAGEFGETRQKMMEILVALGEVYGAGKLIPITGA
                     QVSGASYKTIGRWGLAWLQSLNSRVVVPTVLNPIGMPREGWQEFGIDEEFARHQSEVV
                     EAYRRLGVRLECTCTPYYLRITEYGEHLAWSESSAVVYANSVLGARTNREGGPSALAA
                     AIIGKTPYYGLHIVENRRPQVVVDIEGGTGPHAGHWGAIGHIAGKKVGNRIPIFQGIR
                     PNRDQLKALGAAMAATGAVALFHVDGITPEARVFSFPTDGLDRVTVTEDEIEALFEET
                     AVEAVAVGCPHCSADELREVAELLRGKTTTKPFYVFTAAGVAKSNLDLVSMIERSGAR
                     VITDTCMVVSPRMDEFRSIMVDSGKAFAYVPGMCGAIARIGTRAECVEAATS"
     gene            complement(8146..9387)
                     /locus_tag="PK196_02495"
     CDS             complement(8146..9387)
                     /locus_tag="PK196_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HON40327.1"
                     /translation="MRDFIEMMRERGRVEDIENPCSTVYEAPRVASRTDKIVFFHNLD
                     GHRGVMNLLASRGALAAALGVEERRLVPHLAAATYNGRVLDAGRCGGGVPADLSRIPV
                     MKHFPGDAGRYITAGIVFSSCDGVENASIHRMMVIDDRHVVARLVEGRHTHTMLKTAL
                     ARGERLPVAVTIGTHPLVTFAACTRVPEGKELAYAAELMGGELSVRECGNGVRVPDAE
                     IVLEGYIGQETAPEGPFVDITGTYDPVRQQPVIEFNRIYCKGDPIYHGILPAGDEHKL
                     LMGAPYEPKIYRAVSEVTTVRDVLLTKGGAGYLHAVVKIRKNTQGDGKNAIMAAFAAH
                     TSLKHVVVVDEDIEIHDPSDVEYAIATRVRGDTDIMVITGVRGSSLDPMRLADGTNVK
                     VGIDATMVMGREDEFRRAEWV"
     gene            complement(9380..9934)
                     /locus_tag="PK196_02500"
     CDS             complement(9380..9934)
                     /locus_tag="PK196_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HON40328.1"
                     /translation="MKREFVVGVTGASGAIYARRLLEVLCERARVHIVISDTARQIAG
                     IEGVDLTGFDAVYADNSNLAADIASGSFCCDGMAIVPCSMKTLAAVSNGFADNLITRA
                     ADVCLKERRPLLLLLREMPLSRIHLKNMLAADEAGATVMVASPPFYHHPRTIDDLVDM
                     VVARVLDHLGVRHDLGTRWSGYDA"
     gene            complement(9949..11157)
                     /locus_tag="PK196_02505"
     CDS             complement(9949..11157)
                     /locus_tag="PK196_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON40329.1"
                     /translation="MKIIKDPVHGYIEADTLALQLIDTELLQRLRHISQLGFANLVYP
                     GANHTRFEHSLGTMHLAGLMAGRLGLDEEETRLITTAALLHDTGHGPFSHATEPVMEE
                     YTGRSHHEIDHLLRDGTVAEVLEGAGLDPAEICAIVAGKHHLASIIHGSLDVDRMDYL
                     MRDAHYTGVPYGTVDAQRLIRCTILSETGVALNEGGINAAESLLIARTLMRPAVYFHH
                     VSRIATSMFVHALREELQNVPATDAGELMRMDDAACMERLKHSPHAISRDLARRVYNR
                     DLYKRALYIGDDRVNAVALRQEASPAREREIARAIAETAGIRQDDVLVDIPPIPRALS
                     MEIRVRNTHAMVDIEEVSPIINTLNDTRRHQWRLGVYTPAVHRQVVEQAAMEVLRVKR
                     ATKQDKLVVT"
     gene            complement(11716..12126)
                     /locus_tag="PK196_02510"
     CDS             complement(11716..12126)
                     /locus_tag="PK196_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HON40330.1"
                     /translation="MSVSRFWRKIPQRYNLIGTQCTTCGRYFFPPRTLCPECRRSGRI
                     VNHKFSGEGTVVTYTVIRSASDQFEYATPYVLAIIKLDEGPRLTAQVVCPPEEARIGM
                     RVRGVFRRVAADGESGVIHYGTKFVPVEGEDGLL"
     gene            complement(12131..13291)
                     /locus_tag="PK196_02515"
     CDS             complement(12131..13291)
                     /locus_tag="PK196_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HON40331.1"
                     /translation="MRDVAVIGIGCTEFGEHWSASFRDLFVTAGVLALQDAGVAGDEL
                     DALYVGNMSAGRFVEQEHIGALIADYAGLASNHIPSTRVEAACASGGLAFRQAVIAVA
                     SGMEDIVVAAGVEKMTDVGTGASVDMLASAADREWEGFAGATFPGLYAMIANDYMHRY
                     PLTREQLAQVAVKNHENGARNPIAQFKNRITIDTVLNSSLVADPLRLFDCSPITDGAA
                     AVVVVPLERAREYTDSPVRVLASAQASDTIALHDRRDISTLDATVAAGKRAFDQAGLE
                     HKDIDLLEVHDCFTIAEICAIEDLGFCKKGEAGRLTWEGVTALGGDLPVNTSGGLKAC
                     GHPVGATGIKQVCEIVTQLRGEAGSRQVDAEIGMAHNVGGTGATVVVHILGV"
     gene            complement(13288..>14115)
                     /locus_tag="PK196_02520"
     CDS             complement(13288..>14115)
                     /locus_tag="PK196_02520"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HON40332.1"
                     /translation="YVGSESHPYAVKPTACTVGEAIEATPNMTAADYEFACKAGTAGI
                     QTCMGLVKSGMIQLGIAIGADVAQGAPGDALEYTAAAGGAAFVIGGEDVIADINRTCS
                     FTTDTPDFWRREGQNYPRHGGRFTGDPGYFKHVQGAAMLMFEMAGTTPKDYDYAVFHQ
                     PNAKFPQRVAKMLGFREEQIEPGLVVPRLGNTYSGASMIGLAATLDIAKPGERIFVTS
                     FGSGAGSDAFDITVTDLIESEVFDRAAAPSVERLLANPVYLDYALYAKHKGKIVMQR"
     assembly_gap    14116..14215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14216..14881)
                     /locus_tag="PK196_02525"
     CDS             complement(<14216..14881)
                     /locus_tag="PK196_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40333.1"
                     /translation="MKSGVRHQLAEKMAGEITLSDSPGLALKKWRQSFNIPQGVLAER
                     LEVSPSVISDYESGRRKSPGTAIVGKIVDAILSIDEENGGRYINKFAKILYNEFDEDV
                     IYDIHDYASPVRLTEFAEAIDAAPLCGTLDQTIYGYTVINSLNAILQLSSSEFNRIYG
                     WSTERALIFTEVSTGKSPMVAIRVTPFKPRCVVLQGIGPELVHPLIPKLAERDRISVL
                     CTKM"
     gene            15000..15674
                     /locus_tag="PK196_02530"
     CDS             15000..15674
                     /locus_tag="PK196_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HON40334.1"
                     /translation="MVEPEDLQSLKTIAQLGGCYGPIWLSSQSLGNELGISPQTASRR
                     LIALERQRLIARSMRPDGQYVALTPEGEQVLRREYADYVRIFNPERRQYTLTGTVISG
                     LGEGRYYMSLPYYREQFSASLGFDPFPGTLNVRLDPPSIQVRKHIENAGWIEIPGFTA
                     DDRTFGGARVLPCRIRGHKCAIVVPSRTHYPEDIIEIIAGCELRKALNLNDNDKIEVE
                     ITHDHD"
     gene            15667..16344
                     /gene="ribB"
                     /locus_tag="PK196_02535"
     CDS             15667..16344
                     /gene="ribB"
                     /locus_tag="PK196_02535"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HON40335.1"
                     /translation="MIDAAIDALSRGEFVLLYDFDDRERETDLAIRSDAVTPAHIRQM
                     RKDGGGLICTAVHGEAAKRLGLPFAHEVLRGCGLVEKDGEIPYDIKNHSSFSLWVNHR
                     ETYTGVTDRDRALTITSIAEEVKRSLNGGGHNFAAHFRTPGHVALLRAADRLLDERHG
                     QTELSIALALMADITPAVTICEMLDDETGFALSKEDAMGYARKHGLVFVEGKDVLERW
                     ESCRPAI"
     gene            16631..17278
                     /locus_tag="PK196_02540"
     CDS             16631..17278
                     /locus_tag="PK196_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HON40336.1"
                     /translation="MKKDAVAYLNTRKKEDLAAQMAEVDDFCKYRFRVVEIFHDHRAY
                     ATPPEKRKGFSGMLEYCAANNCRDIIIYDLAGLAKDPDAGLAALKSLNEQYTVYCVHN
                     DFFGFMDEPEQRRAAIADFIEYMEHYRENARKIVPAASKKAKKDRERSIGRPKALTEG
                     QVQALITLREAGTSISQICRMFDVSRSTVSKVLADYPELKGEWKGTRQEPGDGQA"
     gene            complement(17702..18271)
                     /locus_tag="PK196_02545"
     CDS             complement(17702..18271)
                     /locus_tag="PK196_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HON40337.1"
                     /translation="MRSQTTFEREPARRVFAAEFRETRYQFKDDDDEKSPTYVIFPTG
                     IRSNRVFIVGTLTEKQRQGDQNVFYRGRVVDPTGTFFIMAGSYQPEAMQQLARIEPPA
                     FVAVVGKPNLFTTQDGNCLVSVRTESITVVDRETRDLWVLDTARSTLDRLDAFGTTDD
                     SRKAMEEYGTQVPHLYRKIVYEALTQVQM"
     gene            complement(18268..19494)
                     /locus_tag="PK196_02550"
     CDS             complement(18268..19494)
                     /locus_tag="PK196_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HON40338.1"
                     /translation="MNLSEVTERISRKLEAKGAQPDRQKIESRLRRLIEEFGVNLAEA
                     ERSVTADLAREYKVSGIGNNSAELRPISEITPGEWVTIEGKVVALTPPLSPSIAQTGI
                     IADSSGAIRFVTWARANVPPMEFGHWYRIESAVVDEYRGAPNLKIHSGTTISRLEEDA
                     PLLPSITPIADLKPGVGSVRAKVIQDWEVTNDRMLQTGLLGDESGTIKFVIWKDEDKE
                     NLEPDTVYNIYYATVDEYNGRLSLVLNTAMYIADEGDIEVRRNETESRGALVHVAPGS
                     GLIKRCPVEGCNRTLSRQNYCPVHEIQPNFRYDLRIKAVLDDGDRARNVLMQREIVEK
                     LAGITLEEAIQIAETNPLGMDEIFYRIRNAVLGRYYTCNGSEFGGRLLVNSCTPIRFD
                     PGELAALLNRAGGEPA"
     gene            19731..19925
                     /locus_tag="PK196_02555"
     CDS             19731..19925
                     /locus_tag="PK196_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HON40339.1"
                     /translation="MALTADSTIAELLREKPESAQVLFRFGMGCLGCAIANNETIREA
                     AQVHGVPVEEMLAALGIAEA"
     gene            complement(19928..20812)
                     /locus_tag="PK196_02560"
     CDS             complement(19928..20812)
                     /locus_tag="PK196_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HON40340.1"
                     /translation="MRYFVRDGTLFIRGRFRAASTGVGGGIADVTTVLNHTVPHDFQE
                     DPVRRLELITAWHGVFRDYFGLLTAVDMRHLCVLQYDSVTVFITAGVSNPTPSPSTPH
                     TINIIAYSREGMADAALLETIITATGAKAWALHHLGYDFPGTTTDAVVAACERDAEPM
                     HTYAGTLTEIGRRVHEAVLFGLPEALARQQGRVKREGPSFFIYSRYGGDHWVEWQKEN
                     CPYHPCHFAGQRCDYCYCPCYPCGDEDLGEWVNSSNSGSVWGCAGCTLLHIPEVADYM
                     KRNPEATLAELKRFRERL"
     gene            complement(20809..21171)
                     /gene="nrdD"
                     /locus_tag="PK196_02565"
     CDS             complement(20809..21171)
                     /gene="nrdD"
                     /locus_tag="PK196_02565"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HON40341.1"
                     /translation="MHWSPEQLKLVEKYRDLNEIPETERRYKCHTCHLVVDEMPCPNC
                     GETSLEIMCPLDHCDCHHSIIESIEYCPLCGHAVCPECGSHDVMQISRVTGYLQDVAG
                     WNAGKQQELKDRVRYSVV"
BASE COUNT         3966 a         6708 c         6492 g         4481 t
ORIGIN      
        1 atacataaag gttttgcaac gcccggagga tccccttctc gatcggttcc accggccgtt
       61 cgcgggagat cagtcccctg acacccttta agagcgcgat cgaggactcg cgaagatcca
      121 gccgttctag cgccgctgca tactccgccc tgacattgat cgtgaccttc tggagacata
      181 cggtctccac cctgcaccgt gggcagaccc cctcgcccac ctccttgccg cacttcgggc
      241 accggaagac cgggtttgtg tgcgtcccgc agtcacagag gttcttgtag gtgaacgcac
      301 cgcatgcggg gcagcgccgt tcccctgcct ccacctcgat caccccgccg tctgtgttgg
      361 aacgtggttt tgatatacag gccgcctgga acgacctgcg tgacccccct tcgtccccga
      421 ttgggaagag cgagtggggc ggtggacgca tctcccgcgg ttttgacttc ccgggccggc
      481 ccatccgccc tccaatcctg gttccggccc gcgaacggat cctgaaaccc gagaggtgca
      541 tcaccaggtc aagggagtct tccatcgggg catcctgcca ctccggccgc ttctccaggt
      601 ccagcgtcaa ccccaggcat gcaaggagga ccaggtgctc ccggatcgcg atcctgtccc
      661 cggagaggtg gtggggcacc aggagttcct caaggattgc cttcacctcc gggctgttct
      721 ggagcagcag caccccgtcc tcgatcctgc ctccggtgga gaccgcatcc gcaagccggg
      781 cgatatcggc aggggtgata tcgtcccaca tgtatgtgta gtcggggtgg aggggcgcac
      841 cctcgatcgc gaactcgatc gcctcgagtt cattctccgg gtggcgcggc cccccctcga
      901 gcatccacca ctcctcgcag taggccggcg ggataagcgg gtggttgttc tccatgaact
      961 ccccgaaact gatcaggatc tcaccgacgt ccaggatctc ctcgacctgt ccggctatcc
     1021 tcctggcctc tgcagcatcg tccacccttc gcacctcacc gttctggagt ttaaccgtcg
     1081 gcccctggat cgagtctacc ggcacaattc cagccgcctt ccccggccgt tcaatcttca
     1141 tctgcgtccc gaccgccagg aagtctccca ggatatgcat cgttgcaggg ttcaggccgg
     1201 ccgcggcaaa cccggtgttg cgcgcccgac ccaggcgcag ccggaacccg cccctgcgca
     1261 tagggtaggc aaagaccggt cgcccgccga tcaggtcgcg gatgtatttg tctctgggtt
     1321 tgatcgcggc gccggcatcg tcttcctcgc tatttgccgt ggtcccgccg ctgatcaggt
     1381 catcgagcca gtcccagccc tccatcttca tcttccggac gtttttgagg accttcgggg
     1441 ctttcagcgc cagcccttcc gcaacaacca gcgccatacc cccacggacg gtgttcgtct
     1501 ccacccgttc caggttccgg taaccgctca cctcctgctg ctcggtcggt tcgccatcga
     1561 tgcagaccgg acagttctcg atgatcatcc tgagttcttt ctcgctcggg aggtactgga
     1621 gactcatgat gttgttgtac tgccggatct cctcgatgta gcgctccacc tcatcggggc
     1681 gcgggatgta gcggccgatc ccgagcgcac ggcgaacgta gtcccccaca agcacagaga
     1741 gtgcctgggc tgtccccccg gcgctccgga tggggccagc gtagtagatc ttcaggtaat
     1801 ccgtcccgtc atcgttcctc ccgagaccga cctttgcaat cccctccgtc ggggcggcca
     1861 ccacaccctc ggtcaggagg gccatcgccg cgcggatggc gtggtccagg atatcctctc
     1921 gggtagtctc tccaaacttc cttgccacaa aatcgtcgcc tatccggagc gcggcctctt
     1981 cacgggacat ctcctgttca agctcccgga tacgggcggc gataccacgg tagccgagga
     2041 gcgcctccac gcggtcggca aggtcgcttg ccaccgggat ctcgacctcc gttcgggggt
     2101 cgaggccgcg ggcgcgagcg gcggcggcaa gtcgcatcgc agcctcgagc ccggcgtcga
     2161 tctcctcgaa gtatcgggcg atagcgggcg aaacctccat gattaagtat gagttctcca
     2221 ggcatttagc ggactcgttc ccgcccggcg cacccgccga tcccgatcag atatcatccg
     2281 gttaccggtc ttttaccttc agcgcctcgg caagcggccc cgcccccggt tcacccatcc
     2341 tccagaggat gagagcggcg cgttcctgtg tatccgggtc ggggctctcc atcagtcgga
     2401 gcagcggttc gaccgccggt tcaccgatcc ggcatatggc catcacggcg aacggcatga
     2461 ggtcgcgctc agctcttgcg atgatatcga tgagcggttc gatcgcgggt tcgccgatct
     2521 ctgcaagggc cgcggcggcg tatcgccgga gatcgatctc atccgtgcct cgcatagccg
     2581 tgagcagcgg gtcgatcgcc gggctgccta tcagcacaag tgcgttggcg gcataccacc
     2641 ggaggtcgct atcgcccgtc tgcatcacgg cgatgagcgg ttctacggcc tctttcccgc
     2701 tcgccccgag ttttgcggtc gccgcgcggc gcacctctag cgtaccgctc ctgaggtcgg
     2761 cgatcagctc ctcgatccgg atcctctcag tcataccgtt caggtgcttc ttcgcgagag
     2821 ttatgtcttc ctgatgccgc cagaagagcc tccctggcgg cactgcgcac ctcttcccgg
     2881 gcatcgtcga ggccgctgat cagggcctcc accgcttgtc ggtcatcaat cctcccgaga
     2941 acacgggctg cccctgcacg gatgcgccag tcgccagcct cgcggagcgc tcgaacaact
     3001 gcggggacgg caggtctccc gatctggctg agggcggcgg ttgccccctg ccggagacgg
     3061 tcatcgtccg tcccgagcgc gtccatgagg cgcgggaccg ccggttcacc aatatccgca
     3121 agcgccgccg cagctcgcag cctcacatcc tcatcggggt cggaaagcgt cttgatcagg
     3181 gcttcaacag ccctctcccc tccgatcttt ccgagcgcga tcgccgcccc ccaccttacg
     3241 cgctcgtctc cgttgtggat tgcggcgatg agatccccca gtgcagcctc cccgatctcc
     3301 gcgagtgcaa gggccgccct gctccggacg taggtgtcgg ggtcgtcgag cgcggccagc
     3361 agtccgggga cggccgcggg atcgccgatc tccccaaggg cgaccgcggc catccagcgg
     3421 acgtcggcgt cctcgtgccg cagaccctct gtgagcgcgc cgacggccgc ggggccgagt
     3481 ttgccgagcg cttccgccgc cttccagcgg acgccgggat cggggtcggt caggagagca
     3541 gcaagcggtt cgaccgctgc agggtcgccc ctatcaccca ggcacccggc tgcccggtag
     3601 cgcgttccgg ggtcgcggtt tgagaggagg gcgatcagcc ggccggtatc acgctcgcat
     3661 gcagccggtt tcggctcgcc gatgcggaaa tggagttctc ctttgcgcct gcccgcaact
     3721 tcaaatagat aacccagaag ccaggcggcg atgatgaagg tggctgcact tacgaggatc
     3781 gaggcgtcag gcgggccgtg aaagaggtac tcgatggtta cgtggactgc ggcgagcagc
     3841 agtccgacgg caacggcacg gcggcggtac cagaaagcgg ccaggatgag caccaggtag
     3901 aagaagtggg tgtaggcgac cgtgccgaaa gcgaggtcga ataagaccgc gagtgctgca
     3961 acgagaacga tgaccgcggg tctggccaaa ttctctaacc tctctctgcg ccatgcagca
     4021 gtcataccga agagtggatg cggtttgcac ccataaaaga gtttatgcgc tgcggcttgc
     4081 ttcgacttct ggaagcaccg gctgcaatct ttgggcaact ctcccgctta cgcagcatat
     4141 cttcaagacc cctctcctcc ccgttcatgt aagcgatctc tctgcttttt atattcctgt
     4201 gcagaacagg gaatatatgc ctctcttgcc ggtagcagga tgcagagaca ccacctgcag
     4261 gaggctgacc tctgcctgat taccatgccc tctccgtgga cgacgtccag cagaacctcg
     4321 ggacagggcg ttcgggcctc tccacggccg aggcagagga gcggctgcgg cgttacggga
     4381 agaacatcct gcgtgaggag gcgcgtgaga cccggcttca ggtgttttta cggcagttca
     4441 aaagcatcct cataatcata cttatagtcg ctgccgcggt ctcgtttgcg gtcggcgaac
     4501 cgctggatgc cgcggctatc ctgatcatcg tcttcttaaa cgccatcctt gggttcaccc
     4561 aggagtggca ggcgggggag gcgatcgcgg cactcaaaaa gatgctcgtt cagcgtgcgg
     4621 tggttatccg tgatggggag cggcgggaga tcgatgctcc ggagatagtc ccgggagacc
     4681 tcgtcgtcgt ggagatgggt gagcgggttc ccgccgatat ctacctcatc gaggcgacat
     4741 cgctccaggt ggacgaggcg ccgctgacag gcgagtcggc gccggtggac aaggcgccgg
     4801 gctcactccc gccagagacg ccccttgccg agcggagcaa catgatcttt gccggcacaa
     4861 cggtggtcaa cgggcacggc cgggggtatg ttgtcgccac cggcatgcag acggagttcg
     4921 ggctgatcgc cggcctctcc cagcaggtcg aggacgaaac catccctctc gcccggcaga
     4981 tggacaggct cggccgcgac ctcggactga tcgctctcgg cattgccgcg atcgcggttg
     5041 tcatcggtct ccttcagcag cgggaacttc ttgagatgtt ccttgtcggg gtctcgcttg
     5101 cggtggcggt gatcccggag ggtcttcctg cggtcgtgac gctgacgctt gcgctgggcg
     5161 tcaagaacat gatgcagagg aactgtctta tccgccacct gccggcatcg gagacgcttg
     5221 gcgccgtctc tgtgatctgt accgacaaga ccgggacact cacccggaac gagatgaccg
     5281 ttgtccgggt gtggacgccc gaagcggagg tcgatgtcac cggtgcagga tacatcccgg
     5341 agggcgagtt catcctcgac ggcgccccga tcgacccgct cgcagacccc ggtctccggc
     5401 ggtttctgca aacagtcctc atctgtaacc atgcgagcct cggtcacggg gaggagggat
     5461 ggcggatctt cggaacccca accgaggggg cgctcgtggt cgcggcggcc aaagccggcc
     5521 tctcccgtga agacctcccg gaacctgtaa aagagttctc gttcaactcc accaggaaac
     5581 ggatgactgt catctgtccc ggcagggagg gtggcggcta catcgcctgc gtgaaggggg
     5641 cgccggaagt tctccttgaa cgctccaccc ggatgctcag gggtggttcg gcagtcccgc
     5701 tcaccgacgg cctccggaga gaggttctcc tcgcgatcga aggctacgcc tcgcaagggc
     5761 tcagggtgct cggcgcggcc tgccgcccgc tttccaccgc cgcagactgg acaccggagg
     5821 cggtggagga tgacctgatc tttctcgggt ttgcaggtat agtcgaccct ccgcgtccgg
     5881 aggcggcgga ggccatcagc ctctgtcgga gcgccgggat cgacgtcatc atgatcacgg
     5941 gggacaaccc gctgaccgcg tctgccatcg cccgtgttct cggcctctcc agcgagggag
     6001 cactgaccgg cgccgacatt gaatcgctct ccgacgacga actcgcggag cgtctccgga
     6061 cgacaaacgt cctctcccgg gtcacggcgg agcacaaact ccgggtggtc gatctcctct
     6121 cccgcgaccg gaaggtgatc gccatgaccg gggacggcgt caacgacgcc cctgcgctca
     6181 aaaaggcaag catagggata gccatgggca tcaaggggac ggacgcggcc agagagtcaa
     6241 gcgacatggt gctcgtggac gacaacttcg caagcatcgt cgccggcgtg gaggagggga
     6301 gacgggagta tgacaacatc gcccggttca cccgctacct cctctcctcc aatgtagggg
     6361 agatcgtcgc gatcgtcggc gggctcctcc ttggtctccc gctgatactc atccctgtcc
     6421 agatcctctg gatcaacctg gtcaccgacg gattcaccgc cctggccctc ggactcgaac
     6481 ccgctgaacg ggatgtaatg cagcggcggc cgcgcgaccc ggaggagccc atactcaacc
     6541 ggaacgccta cctggtcatc ctcctcctgg gggcctggct cggtctcctg acccttcacg
     6601 ccttcctggg cgtctacgag gtcgacctgg accgggcgcg gacgatggct tttacagggc
     6661 tcgtcatctt tgaggtctac aacgtgctga acttccggtc gttccggttc ccgctctatc
     6721 ggatcggatt cttctcaaac cctgccctga tactggcgat cctcgggacg ctcgccctcc
     6781 aggctgttgt tgtctacgtc cccctgatgc agatcttcct ggggacggcg cctctcacac
     6841 tcgccgactg ggggctgctc gccatcctcg gcctcccggt gctgatcgca ggggaggtct
     6901 acaaggttct ccggttgagg ggcggccggc cctctcgggc tgcgggttga atccggcatc
     6961 gggggttttg agcgtggcag ggcctacgat gtcgcggcct cgacacactc tgccctggtt
     7021 cctatccggg cgatcgcccc gcacatcccg ggaacgtagg cgaaggcctt ccccgagtcc
     7081 accatgatcg atctgaactc gtccatccgg ggcgagacaa ccatgcaggt gtccgtgatc
     7141 acccgggcac cgctccgctc gatcatgctc accaggtcca ggttactctt tgctaccccc
     7201 gctgcggtga agacgtaaaa tggttttgtt gtggtcttcc cccgcaggag ctccgcaact
     7261 tccctgagtt cgtcggcgga acagtgcgga cagcccaccg ctaccgcctc gaccgcggtc
     7321 tcttcaaaga gagcctcgat ctcgtcctcc gtcaccgtca cgcggtcaag gccgtcggtc
     7381 gggaacgaaa agacccttgc ctccggggtg attccgtcga cgtggaagag tgcgaccgcc
     7441 ccggttgcag ccatcgcggc acccagggcc ttgagctggt cgcggttcgg ccggatcccc
     7501 tgaaatatcg ggatccggtt cccgactttc ttgccggcga tatgaccgat ggcgccccag
     7561 tggccggcgt gcggcccggt gccgccctcg atatcgacga cgacctgcgg tcggcggttc
     7621 tcgacgatgt ggagaccgta gtaaggcgtc tttcctatga tcgccgctgc aagggcgctc
     7681 ggcccgccct cccggtttgt cctggcgccg agaacggagt ttgcgtagac aaccgccgac
     7741 gattccgacc acgccagatg ctcgccgtat tcggtgatcc ggagatagta tggggtgcag
     7801 gtgcactcca gcctgacgcc gagcctccgg taggcctcga cgacctccga ctggtggcgg
     7861 gcgaactcct catcgatccc gaactcctgc cacccctccc gcggcatacc gatcgggttt
     7921 aagaccgtcg ggaccaccac ccgggagttg aggctctgca gccaggcaag cccccatctc
     7981 ccgatcgtct tgtaggatgc accgctcacc tgggcgcctg tgatcgggat gagtttgccg
     8041 gcgccgtaga cctcgccgag cgcgaccagg atctccatca tcttctgcct ggtctcgccg
     8101 aactcgcccg cgagaacccg ttcatcatcg ttatcaagat acatcttaca cccactccgc
     8161 tctcctgaac tcgtcctccc ttcccatgac catggtggca tcgatcccga ccttcacgtt
     8221 tgtcccgtcg gcaaggcgca tcggatcgag cgacgagccc cgcacgcctg tgatgaccat
     8281 gatatcggta tccccccgca cccttgttgc gatcgcgtac tcgacgtcgc tggggtcgtg
     8341 gatctcgata tcttcgtcca ccaccacaac gtgcttcaag gacgtatggg ccgcaaaagc
     8401 cgccatgata gcgttcttac catcgccctg tgtgttcttc cggatcttga ccacggcgtg
     8461 gaggtagcct gcgccgccct ttgtgaggag gacatcgcgc accgttgtca cctcgcttac
     8521 ggcgcggtag atcttcggtt catacggcgc ccccatcagg agtttgtgtt catcgccggc
     8581 cgggaggatg ccgtggtaga tagggtcgcc tttacagtag atacggttga actcgatcac
     8641 cggttgctgg cgcacggggt cgtaggtgcc cgtgatatcg acaaacggcc cttccggcgc
     8701 cgtctcctgg ccgatatagc cctcgagcac gatctcggca tcgggaaccc ggacgccgtt
     8761 cccacactct ctgacactga gttcaccgcc catcaactca gcagcgtagg cgagttcttt
     8821 accctcagga acacgtgtgc aggcggcaaa tgtcaccagg ggatgggtgc cgatggtgac
     8881 cgcgaccggg agtcgttccc cgcgggcaag cgccgtcttc agcatggtgt gggtgtgacg
     8941 cccttccacg agcctggcca ccacatgacg gtcgtcgatg accatcatcc ggtggatgga
     9001 ggcgttctcg acgccgtcgc aacttgaaaa tacgatgcct gccgttatgt agcgtccggc
     9061 atcccccggg aagtgtttca tcaccgggat gcgggagagg tctgctggaa cgccgccccc
     9121 gcaccgtccg gcgtccagca cccggccgtt ataggttgcg gccgcaagat ggggaacgag
     9181 ccttcgctcc tcgacaccga gcgccgcggc aagcgcgccc ctgctggcaa ggagattcat
     9241 cacaccccgg tggccgtcga ggttgtggaa gaagacgatc ttatcggttc ggctcgccac
     9301 cctgggagcc tcgtatacgg tcgagcaggg gttctcgata tcctcgaccc tccctcgctc
     9361 tcgcatcatc tcaatgaaat cacgcatcgt atccgctcca tcgggttcca aggtcatgcc
     9421 tgacccccag gtgatcgagc acccgggcca ccaccatatc gacaaggtcg tctattgtcc
     9481 gggggtggtg gtagaaggga gggcttgcga ccatcacggt cgccccggcc tcgtcggcgg
     9541 caagcatgtt cttcaggtgg atccgggata gcggcatctc ccggagcagc agcagcagtg
     9601 gccgcctctc cttgaggcag acgtccgctg cccgtgtgat caggttgtca gcaaacccgt
     9661 tgctgaccgc tgcaagagtc ttcatgctgc agggcacgat cgccatcccg tcgcagcaga
     9721 acgacccgct tgcaatatcg gctgcgagat tgctgttgtc agcataaacg gcgtcaaacc
     9781 ccgtgaggtc aacaccctcg atcccggcga tctgccgggc ggtatcggag atgacgatat
     9841 gcaccctggc gcgctcacag agcacctcga gcaggcgacg ggcgtagatc gccccgcttg
     9901 cccccgtcac cccgacaaca aactccctct tcatgtcctc tacatccgtt atgtaactac
     9961 aagtttatcc tgttttgtcg cccgcttcac ccggaggacc tccattgccg cctgctccac
    10021 cacctggcgg tgcactgcgg gggtatagac tccgaggcgc cattgatgtc gccgggtatc
    10081 gttcagggtg ttgatgattg gcgagacctc ctcgatatcc accatcgcat gggtgttcct
    10141 taccctgatc tccatcgaga gcgcccttgg gatggggggt atgtccacca ggacgtcgtc
    10201 ctgcctgatc cctgcggtct cggctatggc acgggcgatc tcgcgctccc gcgctggact
    10261 tgcttcctgg cggagggcca cggcatttac ccggtcgtcg cctatataaa gcgcacgttt
    10321 gtagaggtcg cggttgtaga cccggcgggc aaggtcgcgg ctgatggcgt ggggtgaatg
    10381 tttcaaccgt tccatgcagg cggcatcgtc catccgcatc agttctcctg cgtccgttgc
    10441 cgggacattc tgcaactctt cccggagggc gtggacgaac atgcttgtcg caatacggct
    10501 gacgtgatgg aagtagaccg ccgggcgcat gagcgtccgg gcgatcagga gcgactcggc
    10561 ggcgttgatc ccgccctcgt tcagtgccac ccctgtttcg gataggatgg tgcaccggat
    10621 caaccgctgg gcgtcgaccg tcccgtaggg cacgccggta taatgggcgt cccgcatcag
    10681 gtagtccatc cggtcgacat cgaggctgcc gtggatgatg cttgcaagat ggtgtttccc
    10741 ggcgacaatg gcgcagatct ccgcggggtc gagccccgcg ccttccagga cctctgcaac
    10801 cgttccatcc cggaggaggt gatcgatctc gtgatggctt cggccagtgt actcttccat
    10861 cacgggttcg gtggcatggg agaatgggcc gtgaccggtg tcgtggagca gggcggccgt
    10921 ggttataagc cgggtctctt cttcatcaag cccgagcctg cctgccatca gcccggcaag
    10981 gtgcatcgtc ccgagagagt gttcaaaccg ggtatggtta gcgccggggt ataccaggtt
    11041 tgcaaaccct aactggctta tgtggcggag cctctggagg agttcggtgt ctataagttg
    11101 gagcgccagg gtgtctgcct cgatgtagcc gtgcaccgga tcctttatga tcttcatcca
    11161 gactgggcag ggagaccccg gtcgggatcg ccgtggagtc tgcccttatt cacctcctgt
    11221 aggtctctac tctgccggat ttaacggggg tcaggggaag ttatggggag tttgagaggt
    11281 tggcggagac ggatctcccg gccggtttcc aggttcgtga cctccggggc tgaaagcggc
    11341 gctgccctcc ctctctcctg cgaggatata taacccggat atcatattaa atcactctca
    11401 tatgctcatg gtctgatgcc ctcgaatcac cacggcggtg aacgtctttt gcggctgacc
    11461 gccccggatc cggatgggag gaggtgcgcg gcatgggggc aaagcgggaa gaccccggca
    11521 aaaaggtggg ggaggtgaat gcggagcccc cctggataca gttccctctt ttgcatctgc
    11581 ttttgcggca tccgggttgc gagaaccagg caaccggacc tgaacaggat aacccgcgcc
    11641 tgtggcgatc cggaagggca cgctcattgt aagcaggaag ccccgcaccg atgagcgcgg
    11701 ggcagttcgc ctatctcaga ggagtccgtc ttctccctcc accggcacga actttgttcc
    11761 atagtggatg acgccactct ccccgtccgc tgccacccgg cggaagacac ccctcacccg
    11821 catccctatc cgtgcctcct ccggcgggca gaccacctga gcggtgagtc gcggcccttc
    11881 gtcgagtttt atgatcgcga ggacgtaggg ggtggcgtac tcgaactggt cgcttgccga
    11941 ccggatcacc gtgtaggtga cgaccgtccc ctcgccgctg aacttatggt ttacgatcct
    12001 gccgcttcgc cggcactcag ggcatagtgt tcgcggcggg aagaagtagc ggccgcaggt
    12061 cgtgcactgc gtccctatga ggttgtagcg ttgtgggatc tttctccaga atcgtgatac
    12121 tgacatcttt tcataccccc aggatgtgca ccaccactgt tgcacctgtc ccgccgacgt
    12181 tgtgcgccat accgatctcg gcgtccacct gccgacttcc ggcctcaccg cggagttgcg
    12241 tgacgatctc gcagacctgc ttgatgccgg tcgcgccgac tggatggccg caggctttca
    12301 ggccgccgct tgtgttcacc ggcaggtcac caccgagggc ggtcacaccc tcccaggtga
    12361 gcctcccggc ctcacccttc ttgcagaacc caaggtcctc tatcgcacat atctctgcga
    12421 tcgtgaagca gtcatggacc tcaagcaggt cgatatcctt atgctcgagc ccggcctggt
    12481 cgaaagcccg tttccctgcc gcaacggtcg cgtccagggt ggagatgtcg cgccggtcat
    12541 ggagcgcgat ggtatcgctt gcctgggcgc tcgcgagaac gcgcaccggt gagtcggtgt
    12601 actccctggc gcgctcaagc ggcaccacca cgaccgccgc tgcaccgtcg gtgatcggcg
    12661 agcagtcgaa gagccgcaac gggtcggcga ccagcgatga gttaagaacg gtatcgattg
    12721 tgatcctgtt cttgaactgg gcgatcgggt tccgtgcgcc gttctcgtga ttcttcaccg
    12781 cgacctgtgc aagctgttca cgggtgaggg ggtaacgatg catgtagtcg tttgcaatca
    12841 tcgcgtatag tccggggaat gtcgccccgg cgaatccctc ccactcgcgg tcagcggcgc
    12901 tcgcaagcat gtccacgctc gctccagtcc cgacgtccgt catcttctcg acgcctgctg
    12961 cgaccacgat atcctccatg ccgcttgcga ccgctatcac cgcctgccgg aatgcaagcc
    13021 cgccagaagc gcaggccgcc tccacccttg tggaggggat gtggttgctt gcaagtcccg
    13081 cgtaatccgc gatcagggct ccgatgtgct cctgttcaac gaatcggccg gcgctcatgt
    13141 tccccacgta gagggcgtcg agttcatcgc cggcaacccc ggcgtcctgg agggccagca
    13201 cccccgcagt gacgaagagg tcgcgaaacg atgcgctcca gtgctccccg aactctgtgc
    13261 acccgattcc gataactgct acgtctctca tctctgcatc acaatctttc ccttgtgctt
    13321 ggcatacagt gcataatcga ggtataccgg gtttgcaagc agtcgctcca cgctcggcgc
    13381 cgccgcgcgg tcgaacacct ctgactcgat gagatcggtg accgtgatat cgaatgcgtc
    13441 acttccggcc cctgagccaa acgacgtgac gaagatcctc tcaccgggct tcgctatgtc
    13501 gagtgtggcg gcaagcccga tcatcgatgc cccggagtag gtgttgccga gcctcgggac
    13561 gacaagaccg ggctcgatct gctcctccct gaacccgagc atctttgcca cccgctgtgg
    13621 gaacttggcg ttgggctggt ggaatacggc gtagtcataa tccttcgggg ttgtgccagc
    13681 catctcaaac atcagcatgg cagcgccctg gacgtgcttg aaataacccg gatcgccggt
    13741 gaaccggccg ccatggcgcg ggtagttctg cccctcacgc cgccagaaat cgggtgtgtc
    13801 ggtggtaaac gagcacgtcc ggttgatatc ggcgatgacg tcctccccgc cgatgacaaa
    13861 cgcggcccca cccgccgccg ccgtgtactc gagcgcgtcc cccggggcac cctgcgccac
    13921 gtccgcgcct atggcaatcc ctaactggat cataccgctc tttacaagac ccatgcaggt
    13981 ctggataccg gctgtaccgg ccttgcacgc gaactcgtag tcggcggcgg tcatgttggg
    14041 tgtcgcctct atggcctcac cgacggtgca ggcggtgggt ttgacggcgt aggggtggga
    14101 ctcgcttccc acgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatct
    14221 ttgtgcagag cacactgata cggtcgcgtt ccgccagttt cgggatcaga gggtgcacca
    14281 gttcgggtcc gatgccctgc agcacaacgc agcgcggctt gaacggcgtg accctgatcg
    14341 ccaccatggg ggattttccg gtcgagacct cggtgaagat gagagcacgc tctgtgctcc
    14401 agccgtatat gcggttgaac tcacttgaag agagttgcag gatcgcattg aggctgttga
    14461 tcaccgtata cccgtatatg gtctgatcca gagtgccaca gagcggggca gcgtcgatgg
    14521 cctctgcaaa ctcggtgaga cgcaccggcg acgcgtagtc gtggatgtcg tagattacgt
    14581 cttcgtcaaa ctcgttgtac aggatttttg caaatttgtt gatgtatcgg ccgccgttct
    14641 cctcgtcgat cgataggatc gcatcaacga tctttccaac gatggcggtt cccgggctct
    14701 ttcgccgtcc actttcgtaa tcactgataa ctgagggaga gacctcaagc cgctcggcga
    14761 gaactccctg cgggatgttg aaactctgcc gccacttctt gagggccagt cccggtgagt
    14821 ccgagagcgt gatctctcct gccatcttct cggcaagttg atgccgcacc ccggatttca
    14881 tgcaggtaat gtcgccacgg caacttaaat aattagcgta gatgacttcg acatttcacg
    14941 aagtggatcc gcgggtgatt ccacaacccg taaatagtat gcatctcaag tatggtataa
    15001 tggttgaacc tgaagacctg cagtctttaa agacgattgc tcagcttggg gggtgctatg
    15061 ggccgatctg gctctcttcg cagtcgctcg ggaatgaact cgggatcagc ccacagaccg
    15121 cttcaaggag gctgatcgca cttgagcggc agcgtctgat cgcccggtcg atgaggcctg
    15181 acggccagta tgttgccctc acccccgagg gcgagcaggt gttgaggcgc gagtatgcgg
    15241 attatgtcag gatcttcaat ccggaacgtc ggcagtacac tctgaccggg acggtgatca
    15301 gcggcctcgg tgaaggacgc tactacatga gcctgccata ctatagagag cagttcagcg
    15361 cgtctctcgg gttcgatcct ttcccgggaa cgctgaacgt caggctcgat cccccatcaa
    15421 ttcaggtccg caagcacatc gagaatgcgg ggtggatcga gatcccgggg tttactgcag
    15481 atgaccggac ttttggcgga gcgagggtgc ttccatgccg cattcggggg cataagtgcg
    15541 cgatagttgt cccctcccgc acacattatc ccgaggatat catcgagatc atcgccggtt
    15601 gcgaacttcg caaagccctg aacctgaacg ataacgataa gatcgaggtg gagattaccc
    15661 atgaccatga ttgatgcagc catagatgcc ctttcacggg gtgaattcgt cctgttatac
    15721 gactttgatg atcgggagcg cgagaccgat cttgctatac gttcagacgc ggtcacgccc
    15781 gcccatatac ggcagatgcg caaggacggc ggtggactga tctgcacggc ggtgcacggt
    15841 gaggccgcaa agaggcttgg tctgccgttt gcgcatgagg tcctgcgcgg ttgcggtctc
    15901 gtcgagaagg acggcgaaat tccgtatgat attaagaacc actcgtcgtt ctccctctgg
    15961 gtgaaccacc gcgagaccta taccggggta acggacaggg accgcgcgct gacgatcacc
    16021 tccatagccg aggaggtgaa gaggtcgctc aacggtggag gtcataactt tgccgcgcac
    16081 tttcggactc ccggtcacgt ggcgctcctc cgtgcggccg accggctgct cgacgaacgt
    16141 cacgggcaaa cggaactctc gatcgcactt gcgctcatgg cagacataac cccggcggta
    16201 acgatctgtg agatgcttga cgacgagacg gggtttgccc tctcgaaaga ggatgcaatg
    16261 gggtatgcga gaaagcatgg actcgtcttt gtcgagggaa aagacgttct ggaacgctgg
    16321 gagtcctgca ggccggcgat ataagagaga cgtcgggcca accagcgttt gcacatggca
    16381 atcgcaggat agttgcaggg ctgcagattc agggtggatg tgtgccattg gtgcgcgtcc
    16441 cccccttcca tggttatccg ggtttttgcg ccccgtttcc ctcttttccg agtagttatt
    16501 tttgggctgt gtttttgtac atctttgctg gagaatagaa atatacgcgg tatctcgcgc
    16561 cgtaccgcca aaaggcgcac tttgtgcgtt ttcaaaaata atatatacga tcgtacggca
    16621 tcataaacgg atgaaaaagg atgcagttgc atacttgaat acaagaaaga aggaggattt
    16681 ggcagcacaa atggcggagg ttgacgactt ttgcaaatat cgatttcgtg ttgttgaaat
    16741 cttccacgat caccgtgcat acgcaacccc tccggagaag cgtaagggtt ttagcgggat
    16801 gcttgagtac tgtgctgcca acaactgccg ggacatcatc atttacgatc ttgcaggact
    16861 cgcaaaggat cctgacgcag ggcttgccgc tctaaagtcc ttgaacgaac agtataccgt
    16921 ctactgcgtg cataacgatt tcttcgggtt catggatgaa ccggagcaga ggagagcggc
    16981 cattgccgac ttcatcgagt atatggagca ctaccgtgag aacgcccgta aaatcgttcc
    17041 ggcagcctca aaaaaggcaa agaaggatag ggaacgttcc attggtagac caaaggcgct
    17101 caccgagggt caggtacagg ccctcatcac gctacgagag gccgggacaa gtatcagcca
    17161 gatctgcagg atgttcgacg taagcaggag cacggtcagc aaggttctcg cggattaccc
    17221 tgaactgaaa ggggagtgga agggcaccag gcaggagcct ggagacgggc aggcataata
    17281 ccggttctgc tccctgccgt gcccccccga aaatacatcg tgccggctat ttcaggtcac
    17341 ggttctgggt tcatgatatg aataacagac gcgggatttg ccctggtgct gccggcacgg
    17401 gcctttatat ggaactctct cttacttctc agctccatcg accccgcccc cccgggggcg
    17461 attgcctcct gctcaaatgc ccgctgggag agcacctcaa agggtcacgg caagcctcca
    17521 gatccgaaga tttaaggcgc gatgactcta tctcctctca atcagatcct tccgcccggt
    17581 ggttctctca tatcaccacc ctctgaatac agaaccaacg ttatgccggg aagagcgcat
    17641 cgagctggat tacagtcggt tgcagcaaaa aaaaagtttc ggaggtaaag cgtggtatct
    17701 tttacatctg cacctgcgtg agggcctcgt agacgatctt tcggtagaga tgcgggacct
    17761 gtgtcccgta ctcctccatg gctttccggg agtcatcagt ggtaccgaac gcatcaagcc
    17821 tgtccagagt tgatcttgca gtatccagca cccataggtc gcgggtctca cgatcaacta
    17881 ccgtgatcga ctctgtgcgg acagagacca ggcagttccc gtcctgggtc gtgaagaggt
    17941 tcggtttacc gacgacggcg acgaaagcag gaggttctat gcgggcgagt tgctgcatcg
    18001 cttctggctg gtacgacccg gccatgatga agaaagtgcc cgtcggatcg accaccctcc
    18061 cgcggtaaaa gacgttctgg tcgccctgtc tttgcttctc tgtgagcgtt ccgacgatga
    18121 acacccgatt acttcggata ccggtcggga agatgacgta tgtagggctc ttctcatcgt
    18181 cgtcatcctt gaactggtag cgcgtctcac ggaattcggc tgcaaaaacc ctgcgcgcgg
    18241 gttcgcgctc gaaagtggtc tgggacctca tgccggctca cctccggcgc ggttgagcag
    18301 tgcagccagt tctccggggt caaaccggat cggggtgcag gagttgacaa gcagcctgcc
    18361 gccgaactca cttccgttgc aggtgtagta gcgtccgagc accgcgttcc tgatgcggta
    18421 gaagatctca tccataccaa gcggattggt ctctgcgatc tggatggcct cttccagggt
    18481 tatacctgca agtttctcga cgatctcgcg ctgcatcagg acgtttctgg cgcggtcgcc
    18541 gtcgtcgaga accgctttta tgcggagatc gtaacggaaa ttgggctgga tctcgtgcac
    18601 cgggcagtaa ttctgccggg agagcgtccg gttgcagccc tcgaccggac agcgctttat
    18661 caggccagag ccaggggcaa cgtggacaag ggctccccgg ctttcggttt cgttccgcct
    18721 gacctcgatg tccccctcat cggcgatgta catcgcggtg ttcagaacca gggagagcct
    18781 gccgttgtac tcgtccactg tggcgtaata gatgttgtag acggtgtctg gctccagatt
    18841 ctctttatcc tcgtctttcc agatgacaaa cttgattgtg ccgctctcgt cgccgaggag
    18901 cccggtctgg agcatccggt cattggtgac ctcccagtcc tggatgacct tggcccgcac
    18961 gctgccgaca ccaggtttca ggtcggcaat tggtgttatc gatgggagga ggggagcatc
    19021 ctcctccaac cgcgagatgg tggtgcccga gtggatcttc aggttcggcg ctcccctgta
    19081 ctcatcaacg acagcggatt cgatccggta ccagtgcccg aactccatgg ggggaacgtt
    19141 cgcccgggcc caggtcacaa aacggatggc cccgctcgag tcggcgatga taccggtctg
    19201 tgcgatcgaa ggtgagaggg gcggggtcag ggcaacgacc ttcccttcga tcgtcaccca
    19261 ctcgccaggc gtgatctcgc ttatcggacg gagttctgca gaattgttcc cgatgccgct
    19321 gactttgtac tcgcgggcaa gatcggcggt cacactgcgc tcggcctcag ccaggttgac
    19381 accaaactcc tcgatgagac gacggaggcg ggactcgatc ttctgccggt caggttgtgc
    19441 gccttttgct tcaagtttcc gggagattct ctctgttacc tcagataggt tcatacatac
    19501 atctccattc tttaattcgg atcctatagt gtattaagcc tcggccgcgc ggtgtgaaag
    19561 tgatcacacc cggccacccc gcgacccgct gtaacggaat aggcgctgac tctggcatct
    19621 cagccatctt ctcgaccggg aggtttggat ttccgtatcg acaggtaatt tgcgcaaatc
    19681 ctgtattgcc gggtcaaatg tatatagagg aaagaacaaa acaaggttat atggcattga
    19741 ctgctgacag tacaatcgcg gaactcctcc gagagaagcc cgaatcggcg caggtactct
    19801 tccggtttgg gatgggttgc cttggctgcg caatcgcaaa caacgagacg atccgggagg
    19861 ctgcccaggt gcacggcgtc cccgtcgaag agatgcttgc tgccctcggg attgccgagg
    19921 cgtaatatta taaccgttcc cggaaccgct tgagttcggc gagagtggct tcgggattcc
    19981 ttttcatgta atcagcaacc tcggggatgt ggagcagcgt gcagcctgcg caaccccaga
    20041 cgctgccgct gtttgaacta tttacccatt cgccgaggtc ctcgtcgcca caggggtagc
    20101 aggggcagta acagtagtcg caccgctgtc cggcgaagtg acatgggtgg taggggcagt
    20161 tctctttctg ccactcgacc cagtggtcgc cgccataacg gctgtagatg aagaaggagg
    20221 gcccttcacg ctttactctg ccctgctgcc ttgcgagagc ctcgggcagg ccgaacagga
    20281 ccgcctcatg aacccgtctg ccgatctctg tcagcgtccc ggcgtaggtg tgcatgggtt
    20341 ctgcgtcgcg ttcgcaggca gcgaccacag catccgttgt ggtcccgggg aaatcgtagc
    20401 cgaggtggtg cagcgcccag gccttggcgc cggttgcggt gatgattgtc tccaggagcg
    20461 ctgcatcagc cattccctca cggctgtagg cgatgatgtt gatggtgtgg ggggtgcttg
    20521 gcgatggtgt tggattgctc acgccggcgg tgataaagac cgtgacagag tcatactgga
    20581 gcacacagag gtgacgcata tcaacggcgg tcaggaggcc gaaatagtcc cggaataccc
    20641 cgtgccaggc ggtgatgagt tcaagacggc gcacggggtc ttcctggaaa tcgtgcggca
    20701 cggtgtggtt gagaacggtc gtgacgtcgg cgatgccacc gccgaccccc gtgctcgccg
    20761 ccctgaaccg gccacgaata aaaagagttc cgtccctgac aaaatatctc atacaacgga
    20821 gtagcggacc cggtccttca gttcctgttg tttgcccgcg ttccagcctg caacgtcctg
    20881 aaggtagccg gtgaccctgc tgatctgcat gacatcgtgg ctcccacact ccgggcagac
    20941 ggcatgaccg cagagtgggc agtactcgat actctcgatg atggagtggt gacagtcgca
    21001 atggtcgagc ggacacatga tctcaagcga agtctcgccg cagttcgggc agggcatctc
    21061 gtccacaacg aggtggcagg tgtgacactt gtagcgccgt tctgtctcgg ggatctcgtt
    21121 gagatcccgg tatttctcca ccaatttgag ttgttcaggg ctccagtgca tggtatcagt
    21181 atctgcggga aaatatataa tataaccctt aagatgaggg agggcatcat acccatacct
    21241 ccaggctgtg gcataacccc atgaactttg cgggaaaaat ctaatttagg ggtgttttct
    21301 tctggcactt ataagtaaca gtctcatttc tgaattctgt tccaacctgc ttccctggtt
    21361 tgataaacct cgccatggac ggattccggg gagtggcatc gccgcgcacc acctctcatc
    21421 tggagccggg gtggccgggg tttttgcaag atctcatttt ggagagatgc agagacggga
    21481 ggaactttgt ccccctctcc cagtcagccc ctcccggggg cggcgactct ccatcgatac
    21541 agtgtcctgg gaggagaccg tctcttcgcg gtctcacccg acactccctg aaattccgtc
    21601 ttcgcccacg gcacggcttc cacgggacaa tcgccgtaca ctgcccgccc cctgtaaggg
    21661 ggaggagacg cggagcgtcg ggcggtgggg gtggtcagcc gcaaaagacg ttcaccgcgg
    21721 cggtgattcg agggcatcaa accatga
//
LOCUS       DAOSZX010000016        21533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1993_length_21533_cov_21.510802, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000016 DAOSZX010000000
VERSION     DAOSZX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_21533_cov_21.510802"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1306)
                     /locus_tag="PK196_02570"
     CDS             complement(134..1306)
                     /locus_tag="PK196_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HON40342.1"
                     /translation="MRVFFIGFGQAGGKVVDMFIEQDKRSQTQSFRGIAVNTARTDLM
                     GLKHIELKDRLLIGQTVVKGHGVGTDNVTGARVTADEIDSIINAIDSRGTHDIDAFVI
                     VAGLGGGTGSGGSPVLARHLKRIYREPVYAIGILPAPEEGRLYSYNAARSLSTLVNEV
                     DNTFIFDNSAWKNEGESVKDAYSRLNDEIVRRFGVLFRAGEVGKAGVGEMVVDSSEII
                     NTLRGGGISSVGYAISEKVSPRTKQQKKSLFSGLMRSNKDKTEQVLTGEDKSAKIIAL
                     VRRAMLGRLTLPCDYSTAERALVLLAGPPEEMDRKGVEKSKSWVEENIAGVEVRGGDY
                     PVLSDYIAAVVVLATIGNAPRITELLETAKSTKEEVLKSKEKRTTMFDDGIEPLFE"
     gene            1431..2663
                     /locus_tag="PK196_02575"
     CDS             1431..2663
                     /locus_tag="PK196_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON40343.1"
                     /translation="MKICVVGGGLSGLVSTLELIGEHEVDLFERRPVPGGCLGSYRIG
                     NYWIEEYYHHCFAGDSALLALFKNLGVIDRLVWLKGTTGYYVDGVVHHLTTPTEILRY
                     PHLTLIEKVRLGLLTLRSRRFDVAALDEVTARDFILDNLGPGLYASFFEPLLKSKFGD
                     RAGEVSAAWLISRIAIRSDRGVGGERLGYLEGGFQHFIARLEEEAARQGASIMSGTPV
                     DEIRRNGEAWLVNGESYDAVVSTLPPQVTATLAGLDIAPVPYQGAACMTLALDRDVTD
                     GIYWLNMKDKAPYGAVVSHTNFAPLEWYGEQIVYLASYFTDQLPEGFEKKMLLDFCRR
                     FSVDEAEVHWHRLAVDPYAGPVYTTGLRKRLPDYEEHGLFMAGMFSPPNYPERSMNGS
                     VIAGREVARRVLERFADG"
     gene            2656..3375
                     /locus_tag="PK196_02580"
     CDS             2656..3375
                     /locus_tag="PK196_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON40344.1"
                     /translation="MAEIEVSAVLPVYNDLAALRIAIPRSLETLEEIAPGRFELIVAE
                     DGSTDGSADFVREYEARDPRVRLLHADERLGRGRALNRAFAAARGSIVCYYDVDLATD
                     MQHLAELIGSVREGYDIATGSRLLPGSTITRSGGREIASRGYNMLVRLILRSRLHDHQ
                     CGFKAFRRGRLLSLLPSVTADHWFWDTEVLVRAQRKDYRIRELPVRWRQGEGTTVRAK
                     DVVEMGSAIVHLWWRLHVEKN"
     gene            3359..4339
                     /locus_tag="PK196_02585"
     CDS             3359..4339
                     /locus_tag="PK196_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HON40345.1"
                     /translation="MLKKISAIVISTLIAAGIIGYMLYRVWDDLMLTLEHAVLPFLVV
                     AVGICIVAWILRGFRYRFILHGLGVEKGVWFSTACILVSQTANLIVPARLGDLVRLLI
                     LKHEDDATYSEGFSSLVVERVFDVMMVAVLGVVALLLVLTVLDVPDWFIIAIALPLGA
                     GCIFFAVILSSGRMNSENRILIAVQQMLDEVRRASLHSRALAALSLSSIFIWVVDVLV
                     CYAVVLMFQESMPFAVVILAIVIGNLVKAVPLTPGGVGTYEVAVALTFGLAGMAAATA
                     TLIAVIDHLIKNLVTLVGGVGSIYYFGDWSVELLKKALKREIEMEDTFGG"
     gene            4329..6329
                     /locus_tag="PK196_02590"
     CDS             4329..6329
                     /locus_tag="PK196_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HON40346.1"
                     /translation="MAGEFLVPVLIWLAIIKVLQLSIWPALDRTLGRLAPAAAYPASV
                     LLFTLVSWYCALSGVPVTLALIPFAAAMIYAGSGRFYTKERLYSSFKWDIAFLIPFLF
                     MLEVRWINPAISSGEKFMDHAILASIMRQPIVPPLDPWYAGGRLDVYYYMGYWMNGAL
                     GLVSGAPSTVAFNLALPTVLGLSVVSLYALGHLILDRHRWLPVLALFIPNPSVIYHIL
                     IGDSWFDVLWGSTRTIENTINEYPLFSMLWGDVHPHVMSFFNQGFLLFLLVFAYLRWG
                     DLSMKGRGLLCGLTALSLGSMPAFNSWDVLVYAPVTLLFGLMVWRRYGKFGRDERRSW
                     MIFGAVPPLAVLAYLPFYLHLNTPGIGGVGIVPEPSAPLEFLLVHGFFLAILTAYCAP
                     DIRKRPYLLLIAVVPALAGYPAAAIAAIPLIYLIARRRFSVIDTLAILGLLIIIGTEF
                     VYLVDKMGGVYCRMNTVFKFYLPAWFMMATASLAIIGEWSQRFGIDRRIPPRLGKALP
                     ALAIILFLVAPFAIGVDFGSGSRTLDGAAYLAVSHPGDAAAIAFLREFPGDQILVEAE
                     GGDYTYYSRVSTFTGIPTIIGMPSHEYMWRGDYGPISERVADVRTIYEQPGRTIDLAR
                     TYNATLLYVGDPERERYNVSLPIGALKVIYDADGVQIYRIPL"
     gene            6520..6888
                     /gene="rpl7ae"
                     /locus_tag="PK196_02595"
     CDS             6520..6888
                     /gene="rpl7ae"
                     /locus_tag="PK196_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HON40347.1"
                     /translation="MATTYVKFEVPEEIQNKALEALEIARDTGKVKKGSNETTKAIER
                     NIAQLVLIGSDVEPQEIVMHLPPLCDEKRIPYVYISKQNHIGSASGLEVGSAAAAIVK
                     AGKAKDLVDEISKQIAALRA"
     gene            6899..7108
                     /locus_tag="PK196_02600"
     CDS             6899..7108
                     /locus_tag="PK196_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HON40348.1"
                     /translation="MADDGTPAEVIEIIGITGMHGEARQVKCRVLDGPNKGRIITRNT
                     VGPIREGDILSLLETEREAKKLSRR"
     gene            7111..7302
                     /locus_tag="PK196_02605"
     CDS             7111..7302
                     /locus_tag="PK196_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HON40349.1"
                     /translation="MVEKYVCSFCGEVLEPGTGKMFVRRDGTIYYFCSSKCQNNYRLG
                     RVPRRVEWTAAGRKARGKE"
     gene            7309..7758
                     /gene="ndk"
                     /locus_tag="PK196_02610"
     CDS             7309..7758
                     /gene="ndk"
                     /locus_tag="PK196_02610"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HON40350.1"
                     /translation="MERTFVMVKPDGVQRGLIGEIVSRFEAKGLKLLAARLEALPEDR
                     VVEQYREHMEKPFFPGLAAYIMSGPCFLMAWEGKDAVAVVRRMVGATNPAEAAPGSIR
                     GDLGLDIGRNVIHASDSHESAARELGIHFAPGELVDYSRIDEPIIYE"
     gene            8162..8815
                     /locus_tag="PK196_02615"
     CDS             8162..8815
                     /locus_tag="PK196_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HON40351.1"
                     /translation="MADLLSFILLSLSSIIIVVNPLAATLIFVSLTGTMDQAAKLKIA
                     REASQFALAVLLIFTFGGGWILQIFGISIEAFRIAGGVLLFGIGMDMVYAKTSRTKMT
                     ATEKYEGQDTDDIAIMPLAIPMIAGPGAITSVIVLMNEAMPMGLAAIAMVPLAVIVAI
                     GITYYMMKNADVIVRRIGQREYRAVNRLMGMLLIAIAVQFIIVGIKAAFPLLSGGSV"
     gene            8812..9012
                     /locus_tag="PK196_02620"
     CDS             8812..9012
                     /locus_tag="PK196_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40352.1"
                     /translation="MRSNLAQIGAILASTCIIASGILVAVQMFAITVPFAPYIMVALS
                     ILIVIGAAMLIHSSRSLETSLS"
     gene            9012..9815
                     /locus_tag="PK196_02625"
     CDS             9012..9815
                     /locus_tag="PK196_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HON40353.1"
                     /translation="MMTAIALAQVATGARRGRVGVLDAAARMADEAAGRGASLICFPE
                     QFVTGWSPDVAPGAGEPLDGPLATAFSHIAERCGIAVAGSIIESSVGCRPKNTVVVTG
                     ADAKLLAAYAKIHLFSPDGEDLAYEAGDRIATFTLDGTVFGLAVCYDLRFPELFRIYA
                     LAGAECVLVPAAWPCSRLSHWETLLPARALENRYYVAGVNTPGMPGGACCGGSLAADP
                     DGVIIGRCGAGEEVLIVEIDPAAVHDARSRLPSLSDRRSELYQRLLSRL"
     gene            9825..10571
                     /locus_tag="PK196_02630"
     CDS             9825..10571
                     /locus_tag="PK196_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HON40354.1"
                     /translation="MRGDQHVSLSLTTAALLIAPNLSTIDPFTAVVLLFGTFVGSVAP
                     DADAADAAIFNGRISGAKGKRGQVINGLAVVLPIFGYTIRYLIYYPISLVFTLLLRKN
                     YRHRHRGLLHSLPGVGLTTLILSAYLAVLLAWLGLSLALLPAFGCGFFGGSVLHLLED
                     ACTPSGVAWLYPLSRRRVSGRVRAQRFFEVRPTIFAAVLATAAAGVLIAPFVTDLTTD
                     ELRFIAFAAALTLWLLFLLVSGVRRERRCG"
     gene            10616..11818
                     /gene="thrC"
                     /locus_tag="PK196_02635"
     CDS             10616..11818
                     /gene="thrC"
                     /locus_tag="PK196_02635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HON40355.1"
                     /translation="MYRLVCVHCGAAYPQDQIIYTCSRCGHLLSVEYDLDDIEVSRGE
                     WNRRPLSVWRYREILPVSGEPVTLQEGGTPLYHLKKIGKDLGLPELYAKHEGMNPTGS
                     FKDRGMTVGVSMALELGMKSVACASTGNTSASLAAYAAKAGVPCVVLLPAGKVALGKV
                     AQALMHGARVISIRGNFDRALEMVHELCVSHGLYLLNSVNPYRLEGQKTIGFEVIDQV
                     GGVPDRMVLPVGNAGNISAVYKGLKEFEALGFIDRLPMMTGIQAAGSQPVVRAIEQNL
                     NVLIPESNPETVATAIRIGAPVNAEKALVAIRATGGTAAAVTDEEILAMQKDLARKEG
                     IGVEPASAASVAGVRRLVEQGLIDRDERIVCVVTGHLLKDPETVVRQCEPPVEIDADI
                     SSLLSVLR"
     gene            11758..12372
                     /locus_tag="PK196_02640"
     CDS             11758..12372
                     /locus_tag="PK196_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HON40356.1"
                     /translation="MRTSSRDRRGHIIAALCLALILVSAPAAAQEAVFQVLPDGTAYE
                     ASIEVSGDTYTLWTPGLLGERVPLQVEGLEVLGPKGAVEYREEGRGVITFPEGNYTIS
                     YRVPVRNNQFVAAFDTPYNVTVLLPPVFKVDNPLIGMVSTGGVVSPGPNETTEIAWDR
                     AKVVEVRFYTPEREMLLTTFGTVWLAVALVLIFPFLFSRRRGGR"
     gene            12369..12944
                     /locus_tag="PK196_02645"
     CDS             12369..12944
                     /locus_tag="PK196_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HON40357.1"
                     /translation="MIEVLAASFLIGFSGAASPGPLTASVLGIGSRQPLRFVTGLVAG
                     HGVPEAVMVAAIACGVRDVPHIDLVALLGSCVLIALGAMQFLRAGDTLVVSEETRMPV
                     AFGLACTLGNPYWWVWWLTFGVGFLALHPSFVAFYIGHIGADIVWLGLLAVAVSRGAN
                     VLGRHYKKVVQASGLAMMLFGLYFILSVLST"
     gene            complement(12919..13827)
                     /locus_tag="PK196_02650"
     CDS             complement(12919..13827)
                     /locus_tag="PK196_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON40358.1"
                     /translation="MTGLPEGCDLFTPGHRACGGCGPALAARLLLKATGENVIVVNST
                     GCMEVFSTPYPETTWGVPWIHSLFENAAAVASGIEASLKKQGRSEKVVCICGDGATFD
                     IGMLCISGAFERGHDITYVCYDNEAYMNTGIQRSGATPYAASTTTSPAGACSPGNVRQ
                     KKDMPAILAAHGAPYVATASIAYPADFEKKVRRAINTPGPCYIQVHTPCCTGWGFESG
                     ETITMAKLAIETGLWVNYEMANGVVEKAKKVKRKPVEEYLSRQKRFRHLFKPSRRDDL
                     IAEIQRIADANAERFGIDIRSKEPRE"
     gene            complement(13828..14982)
                     /locus_tag="PK196_02655"
     CDS             complement(13828..14982)
                     /locus_tag="PK196_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HON40359.1"
                     /translation="MLEIMEGSHAVAEIVKRCRPQVIAAYPITPQTHIVEALAQMVAD
                     CELDADYITVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNVAGLRQPVVM
                     TIANRSLGAPLSIWNDQQDSISLRDSGWLQVYAEDNQEAVDLHMIAYRVCEDHDVLLP
                     AFVCFDGYVLTHTYEPVSIPTQEEVDAYLPAFNPYQRLDASNPLSFGMYATPDYYMEF
                     RYEIDRAQHRAKEVFAKAGHEFAKQFDRDYSAPVEGYRLEDADTAIVAMGSICGTAKD
                     AVDEMRDAGKNAGLLKIRMFRPFPAQEIVDALKGVSTVAVLDRNISLGSGGGVGTEVK
                     AALSGSGTAVYDYIVALGGRDIRKKDIAAIVDLAEEGRGDMFYGLRTEVL"
     gene            complement(14982..15242)
                     /locus_tag="PK196_02660"
     CDS             complement(14982..15242)
                     /locus_tag="PK196_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40360.1"
                     /translation="MALRVGCAAPPGKALENKTGTWRVFKPVFDPETCNRCGICAQFC
                     PEGCIRETDEGMFEPDLDYCKGCGICEEVCPKKSIRMEKEEK"
     gene            complement(15243..15788)
                     /locus_tag="PK196_02665"
     CDS             complement(15243..15788)
                     /locus_tag="PK196_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HON40361.1"
                     /translation="MRELRIHGRGGQGSVTAAELIACAAFEGGVFSQAFPAFGVERRG
                     APVQAFVRFSDRKIRLRSQVYEPDYIIVQDSTLIRDVDVFNGIKPGGIAIINTEKTAS
                     DFRVPEGVTVYTIDATTIALEELGLPITNTALMGAFAAATGEIGLEPLKGALRSRFAG
                     SLAEKNIRAAERAYKLVGGAA"
     gene            complement(15919..16536)
                     /gene="hxlB"
                     /locus_tag="PK196_02670"
     CDS             complement(15919..16536)
                     /gene="hxlB"
                     /locus_tag="PK196_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HON40362.1"
                     /translation="MPQEFPDITDFMLQMTSSLEEVARNIDRESANQLLDKILNAKRI
                     YLAGAGRSGLVARAFAQRLMHLGFESYVIGETITPAFRSGDLLIAVSGSGETRSVVDA
                     CETAKELGGAVCLITSTPDSSIGRIADCIVEIGNNQLKDQGLPSDFEIRQLTGQYRSV
                     SASFAPLGTLFETAALVFSDAVVSALMEIRQCTPEDLKSRLANVQ"
     gene            complement(17007..17879)
                     /locus_tag="PK196_02675"
     CDS             complement(17007..17879)
                     /locus_tag="PK196_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HON40363.1"
                     /translation="MFIGIDHGTTAMRFSCGEAEFKISREEARYFSAGDLARLCPLDE
                     IEGVAVCYSMGDGITAITDIRRVENRGVVSQEGAGKHIGGGTRVYDAIRESGLPAIVI
                     PGLHRGSPTDPRFKAYSHQASPEKIGIAYHVVHDLGDDVVVCDASSNTVTLLVTGGRI
                     TGAFDACIFAPGTEHGALDVNAIRRIDRGEITANDAFLHAGVDYTMDADLRLETMAMF
                     AAMECAAMLLLNPDAKVALAGGMAPAIAAEVEALLSRKVAVYDEWCAARGLVRIARDV
                     FSGATGILGLAVER"
     gene            complement(17948..18820)
                     /locus_tag="PK196_02680"
     CDS             complement(17948..18820)
                     /locus_tag="PK196_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HON40364.1"
                     /translation="MNLRPDLREYAGIYLRGLMMGACDIIPGVSGGTIALITGIYERL
                     VGAIGSIDLNLAKDLLRGDTVALRADLERIDIPFLIVLLAGISTAFLLMSGVILTLLA
                     DYAVETYSFFLGLIFASAIAIFLDIRSHSHATVAFLFIGAVVGYLIGGLGHMDIGHSL
                     PILFFTGMAALCAMILPGISGAYITLVLNQYEFMLAALRAFLLPEIIAYIAGGVTGLL
                     LFVKALKYLLSTYHGLMLALLTGLMLGSARMLWERGMAAGEMLPGGLIFFLAGLVVVG
                     AVEYAKRRYQANTA"
     gene            19120..20049
                     /locus_tag="PK196_02685"
     CDS             19120..20049
                     /locus_tag="PK196_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HON40365.1"
                     /translation="MFCIVTGGAGFIGSHIVDALVAAGDKVLVIDDFSAGSEKNLAHH
                     QAGGQVTSIRQNLLDDGWQERFCGADRVYHIAADPDVRQSAVNPDSQIRNNIITTHRV
                     LEAMRVHGVPELVFTSTSTVYGDATVIPTPETYTPLEPISVYGATKLACEALISSYCH
                     SFGMTSWIYRFANIIGERSGHGVIPDFIRKLRENPRELEILGDGRQTKSYLEVRECVR
                     AVQFGIEHSHDPVNIFNIGSEDWIDVVTIADIVTEEMGLSGVRYRFTGGARGWVGDVP
                     RMQLSVEKLKNLGWRCRTTSEESVRSAVRAMLG"
     gene            20074..21228
                     /locus_tag="PK196_02690"
     CDS             20074..21228
                     /locus_tag="PK196_02690"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HON40366.1"
                     /translation="MKYIVILGDGMADEPLAELDGRTPLEYAETPNMDRIAREGRCGM
                     LRTVPEGFEPGSDIANLSVLGYDPRVSYTGRGPLEAASMGIELGEGEMAYRCNLVTVR
                     DGLMVDFNAGHISSAEGAALLRDLDAVLGGVRVYPGISYRNLLVLPEGRGSLTTPPHD
                     IVGRPIQEYLPRGDDAAALLECMERSCEVFADHPVNRRRVLEGKIPATGIWPWSGGKR
                     PSLEPFREKYGLAGGVISAVDLLGGIARLAGMEVIRVPGATGFIDTDYKAKARYAVDA
                     LDRLDFVYMHVEAPDEAGHMGSVEEKVRAIERLDEAIGIILERPETVVAVLPDHPTPI
                     RCRTHTADPVPFAVLGKGKDGVKGFSEREARKGSFGLMEAAAFLPMLFSP"
BASE COUNT         4108 a         6385 c         6388 g         4652 t
ORIGIN      
        1 gagtgggccg gtggcagggt tgatggtctc ggttttaatg ttaaacgccg atgccccctg
       61 cacggcacag aggagtgcca gcagaaggat aaaccacctt acgatgcttg ccttctccat
      121 aatagccttc ccctcactca aacaaaggtt caattccgtc gtcgaacatg gtcgtacgct
      181 tctcctttga tttaaggact tcttcctttg tggactttgc ggtctcaagg agttcggtaa
      241 tccggggagc gttgccgatc gtcgcgagga ccaccactgc cgcgatgtag tcgctcagca
      301 cagggtaatc cccgccacgc acctcaacgc cggcgatgtt ctcctcaacc cagctctttg
      361 acttctccac gcccttgcgg tccatctcct ctggcgggcc ggcgagaagg acaagagccc
      421 gttcagccgt cgagtagtcg cagggcaggg tcaggcgccc gagcatcgcc cgcctgacaa
      481 gagcgatgat ctttgctgac ttgtcctcac ctgtcagcac ctgctcggtc ttatccttat
      541 tgctgcgcat caggccggag aagagactct tcttctgctg ttttgtgcgc ggactgactt
      601 tctcgctgat ggcgtaaccg acagaactga tgccgccgcc ccgaagggta ttgatgatct
      661 cgcttgaatc cacaaccatc tcaccgacac ctgctttgcc gacctcaccc gcccggaaga
      721 gcacgccgaa ccgccggaca atctcgtcat tcagtcttga ataggcgtcc ttgacactct
      781 cgccctcatt tttccaggcg ctgttatcga agatgaatgt attgtccact tcgttcacca
      841 gggtgctcag ggaacgggca gcgttatacg agtagagtcg gccttcttcg ggggcgggca
      901 ggatgccgat cgcgtagacc ggttcccggt atatgcgctt taagtggcgg gcaaggacag
      961 gcgaaccacc cgagccggtt ccgccgccaa gaccggctac aatgacgaag gcgtcgatat
     1021 cgtgggtgcc gcgtgaatcg atggcattga taatggaatc gatctcatcg gcagtcaccc
     1081 gcgcgccggt cacgttatcg gtgccgacac cgtggccttt caccaccgtc tggccgataa
     1141 gcagccggtc cttgagctcg atatgcttga gccccatcag gtcggttcgt gctgtgttta
     1201 ctgcgatgcc cctgaaactc tgggtctgag agcgtttgtc ctgttcgatg aacatatcga
     1261 cgaccttgcc cccggcctgg ccgaatccta tgaagaagac cctcatctag tttcacaccc
     1321 ttatattgac tgattaaata tatccatgct tatgtataaa gaccatgcat acagagttag
     1381 aagtttttat caaaaactac tcttatacct ttcatgtaca ggggtgatca atgaagatct
     1441 gcgttgtggg gggaggttta tccggtctgg tctcgacact tgaactaatc ggggaacatg
     1501 aggtagatct cttcgagaga aggcctgttc cgggaggatg ccttggatcc taccggatcg
     1561 gcaactactg gatcgaggag tattaccacc attgttttgc cggagattct gcgctgcttg
     1621 cactcttcaa gaatctcggg gttatcgacc gactggtatg gctcaaaggg accaccggtt
     1681 actacgtcga cggggtagtc caccacctaa ccaccccgac cgagatcctg cgctaccccc
     1741 acctcacgct catcgaaaag gttcgcctgg ggctgctgac actgcgctcc cgccggtttg
     1801 acgtggcagc gctcgacgag gtcaccgcga gggactttat ccttgacaac ctggggccag
     1861 ggctctatgc ctcgtttttt gaaccgctcc tgaagagcaa gttcggagac cgggcggggg
     1921 aggtctcagc ggcatggctg atatcccgca tcgccatcag atccgaccgg ggtgtcggag
     1981 gggagcggct tggttatctg gagggaggtt tccagcactt cattgcgcgg ctcgaggagg
     2041 aggcggctcg acagggtgca tcgataatgt ccgggacgcc tgttgatgag atccggcgga
     2101 atggcgaagc ctggctcgta aacggtgaat cttacgacgc tgttgtatcg accctccctc
     2161 cccaggtgac cgcaactctt gccggtctgg atattgcccc ggtcccctac cagggtgccg
     2221 cctgcatgac cctggcgctc gaccgtgacg ttaccgatgg gatatactgg ctgaacatga
     2281 aggacaaggc cccatacggt gcggttgtct cgcacaccaa ctttgccccc cttgagtggt
     2341 acggcgagca gatcgtttac cttgcgtcct acttcacaga ccaactcccg gaaggctttg
     2401 agaagaagat gctcctcgat ttctgcaggc gtttctccgt tgacgaggct gaggtgcact
     2461 ggcaccgcct ggcagtcgac ccatacgcag gacccgttta cacgaccggc cttcggaagc
     2521 ggcttccgga ctacgaggag cacggtctct tcatggccgg gatgttcagc ccgccgaact
     2581 acccggagcg gagcatgaat ggttcggtca tcgccgggcg ggaagtggca agaagggttc
     2641 ttgagaggtt tgcggatggc tgagatcgag gtgagtgctg tcctgccggt ctacaacgat
     2701 cttgcggcgc tccgaatcgc cataccgcgg tcgcttgaga cgctcgaaga gatcgcgcca
     2761 ggcaggttcg aactgatcgt cgccgaggac gggagcaccg atgggagcgc ggattttgtc
     2821 agggagtatg aagcccggga cccgcgggtc agactgctcc atgccgatga gagacttgga
     2881 cggggccgtg ctctcaaccg ggcgtttgcc gcggcgagag gatcgatcgt ctgttactac
     2941 gacgtagatc tcgcaaccga tatgcagcac ctcgctgaac ttataggctc cgtccgggag
     3001 ggctatgata tagcaacagg ctcccgcctc cttcccggga gcaccatcac ccggagcggc
     3061 ggccgcgaga tcgcaagcag gggctacaac atgcttgtgc gcctgatcct ccggagcaga
     3121 ctccatgacc accagtgcgg gttcaaggcg ttccggcgcg gccgcctgct ctctctgctc
     3181 ccatctgtca cggcggatca ctggttctgg gacacggagg tgctggttcg ggctcagagg
     3241 aaggattacc ggatacggga gctccctgtg cggtggcggc agggcgaggg gacaaccgtt
     3301 cgcgcgaaag atgtcgttga gatgggatcg gcaattgtgc atctctggtg gcggcttcat
     3361 gttgaaaaaa attagcgcca tcgtcatatc caccctgatc gcggcaggga tcatcgggta
     3421 tatgttatac cgcgtgtggg acgacctcat gctgacgctc gagcatgcgg ttctgccgtt
     3481 cctggttgtg gctgtcggga tctgcatcgt tgcctggata ctccgtggtt tccgatacag
     3541 gttcatcctc catggactcg gtgtcgaaaa gggtgtctgg ttctcgacag cctgcatcct
     3601 tgtatcccag acggcgaacc tcatcgtccc ggcgcgtctc ggcgaccttg tgcggctgct
     3661 catcctcaaa cacgaggatg atgccaccta ctccgagggg ttctcctccc ttgtcgtcga
     3721 gcgggtcttc gatgtcatga tggtcgcggt gctcggtgtc gttgcgctcc tgcttgttct
     3781 cacagttctt gatgtccccg actggttcat catcgccatt gcgctgcctc tcggtgcagg
     3841 ctgcatcttc ttcgctgtta tcctcagttc gggcaggatg aactccgaga accggatcct
     3901 catcgccgtc cagcagatgc tcgacgaggt gagacgggcc tcccttcatt cccgtgcact
     3961 cgcggcgctc agtctctcat cgatttttat atgggtcgtc gacgtgctgg tctgctacgc
     4021 ggtagtcctg atgtttcagg agtcgatgcc gtttgctgtg gtcatccttg cgatcgtaat
     4081 cggcaacctt gtaaaagcgg tgcctctcac gccgggcggc gttgggacat atgaggttgc
     4141 ggtcgcactc accttcgggc tggcagggat ggcggcggcc acggcgactc tgatcgcggt
     4201 catagatcac ctgataaaaa acctggtcac gctcgtcggt ggtgtcggtt cgatatacta
     4261 cttcggcgac tggtcggtcg aactcctcaa gaaggctctc aaaagagaga tcgagatgga
     4321 ggatacgttt ggcgggtgag tttctcgtcc ccgttctgat ctggcttgcg atcatcaagg
     4381 tgctccagtt atccatctgg ccggcgctcg accgcaccct tggccgcctc gctccggccg
     4441 ccgcctaccc ggcatccgtc cttctcttca cactcgtctc ctggtattgc gctctttccg
     4501 gtgtcccggt cacgcttgcc ctaatcccct ttgctgcagc gatgatctat gccggttcag
     4561 gcagattcta cacaaaggaa cgattgtatt cgtcgttcaa atgggatatc gccttcctga
     4621 tccccttcct cttcatgctc gaggtgcgct ggatcaaccc ggccatctcc tccggcgaga
     4681 agtttatgga ccacgccatc ctggcctcga tcatgcgcca gccaatcgtc ccgccgcttg
     4741 atccctggta tgccgggggg aggctcgacg tctactacta catgggttac tggatgaacg
     4801 gggcgctcgg gcttgtgtcc ggtgcgccat ctacagtggc gttcaacctt gccctgccga
     4861 cggtgcttgg cctctctgtc gtctcgctct acgctctcgg ccacctcatc ctcgaccgcc
     4921 accggtggct cccggtgctt gcgctcttca tcccgaaccc ctccgtaatc tatcacatcc
     4981 tgatcggaga ctcctggttt gacgttctct ggggcagcac caggacgatt gagaacacca
     5041 tcaacgagta tccgctcttc tccatgctct ggggcgacgt tcatcctcat gtcatgagtt
     5101 tcttcaacca ggggttcctg ctctttctac tggtttttgc ctatctccgg tggggtgatc
     5161 tctctatgaa gggaaggggt ctcctctgcg ggcttaccgc cctctccctc gggtcaatgc
     5221 ctgctttcaa ctcctgggac gtcctggtct acgccccggt cacgcttctc ttcggtctca
     5281 tggtctggcg ccgctacggg aagttcgggc gagacgagag gaggtcgtgg atgatcttcg
     5341 gtgcggtgcc gccgcttgcc gtcctcgcat acctcccctt ctacctccac ctcaataccc
     5401 ctggcatcgg gggagtcgga atcgtccctg aaccctctgc acctcttgag ttcctcctgg
     5461 ttcacgggtt cttcctggct atcctcaccg cttactgcgc tccagatatc agaaagcgcc
     5521 cctacctgct gcttatcgcg gtggtgcctg cgcttgccgg atacccggcc gcggccatcg
     5581 ccgccatacc gctgatctac ctgatcgccc gccgccggtt ctcggtcatc gatacgcttg
     5641 ctatcctcgg gctcctgatc atcatcggca cggaattcgt ctacctggtg gacaagatgg
     5701 gcggggtcta ctgtcgtatg aacactgtct tcaagttcta ccttcccgcc tggttcatga
     5761 tggcgacagc gagccttgcc ataatcgggg agtggagcca gcggttcggg atcgaccgcc
     5821 ggatacctcc ccgcctcggg aaagcccttc cggccctggc tatcattctc ttcctggttg
     5881 cacccttcgc gatcggtgtt gacttcggtt ccgggagccg cacgctcgac ggggcggcat
     5941 accttgcagt ctcacacccc ggcgatgcgg cggcgatcgc ctttcttcgg gagtttcccg
     6001 gtgatcagat cctcgtcgag gcagagggcg gcgactacac ctactactcc cgtgtgtcaa
     6061 cattcaccgg gatcccgacc atcatcggga tgccctctca cgagtacatg tggcggggag
     6121 attacgggcc gataagcgag cgggtcgctg atgtgaggac gatctacgag cagccaggtc
     6181 gcacaatcga ccttgcgcgc acctacaacg ccacactcct ctacgtgggt gatccggagc
     6241 gcgaacgcta caacgtctct ctccccatcg gggcacttaa ggtcatctac gatgcggatg
     6301 gagttcagat ctatcggatc cccctgtgaa ccaccgatga acgtgtccct ttgaaccggg
     6361 cgcggggatg gtggtggggt cgcagtgttc tccattctgc ggctgagtga caaaccttta
     6421 tcactcctct tattgacata atagagcaca ttcgatcagc tacgctactg atgaatgatg
     6481 atggagcagc agaggggctg cccctgaaag aggtgagtta tggcaacgac ttatgtaaaa
     6541 ttcgaggttc cggaagagat ccagaacaag gcccttgagg cccttgagat tgcaagggat
     6601 accgggaagg tgaaaaaagg gtccaacgag acgacaaaag ccatcgaacg gaacattgca
     6661 caactggttc ttatcggttc tgatgttgag ccgcaggaga ttgtgatgca tcttccgccg
     6721 ctctgcgatg agaagcggat tccgtacgtc tacatctcga agcagaacca cattggctcc
     6781 gcaagcggtc tggaggtagg ctcggcagcc gcggcaatcg tcaaagccgg caaggcaaaa
     6841 gacctggtcg atgagatctc aaagcagatt gctgcactga gagcgtgagg ggtaaaccat
     6901 ggcagacgac ggaacgcccg cagaagtcat cgagatcatc ggcataaccg ggatgcacgg
     6961 cgaagccagg caggtgaagt gccgtgttct tgacggccca aacaaagggc ggatcatcac
     7021 ccgcaacacg gttggcccga tcagagaggg agacatactc tcactgcttg agacggagcg
     7081 tgaggcaaag aaactctcga ggcggtaacc atggtcgaga agtacgtctg cagtttctgt
     7141 ggagaggttc tggagcccgg caccggcaag atgtttgtgc gcagggatgg gaccatctac
     7201 tacttctgca gcagcaagtg ccagaataac tacaggctcg gtcgggtgcc gcgtcgcgtc
     7261 gagtggaccg cagccggaag gaaggctcgc ggtaaagagt gatctcccat ggagcgcaca
     7321 ttcgtgatgg tcaagcccga tggcgtccag cgcggactca tcggggagat cgtctccagg
     7381 ttcgaggcga aggggctcaa gctcctagcg gcgagacttg aggctctccc cgaagaccgg
     7441 gttgtcgagc agtaccgtga acacatggag aagccattct ttcccggtct agcggcctac
     7501 atcatgagcg gaccctgttt cctgatggcc tgggaaggaa aggatgcggt cgcggttgtc
     7561 cgcaggatgg ttggcgccac caatcccgcc gaagcagcgc ccggatcaat ccgcggtgat
     7621 ttagggcttg atatcggccg gaacgttatc cacgcgtccg actcccacga gagcgctgcg
     7681 cgggaactcg ggatccactt cgctccgggg gaactggttg attactcccg gatcgatgaa
     7741 ccgataatct acgagtgaat gcaggtgccg ccggataccg gtggcagaac atatttttgg
     7801 aatcacgata ctgtctggaa ggtgtgggtg ggcgcctcaa gtcgtgcgcg gtgacgatcg
     7861 aaccagttca ttggtgctcc cgaaccgctc gcgtcgatcg tccttcgccg agaacccagc
     7921 ggcctgtggg tgcgggaatt ctgtggcaat tgctcccctg gaagccgttt tcggttacgt
     7981 ggaccaaatt tctgcgggtc aaagcggaac tgaagaagag cacccctttt cccccttccc
     8041 ccgcgcaaaa acccccggaa aaatccttta ctatggcagg gtttcaggga aacggatcga
     8101 ctatcccttc aacaaaccac aagtgctaat atcggcctta ttgcatacct gatgactgca
     8161 gatggcagat ctcctgagtt tcatcctgct gagcctctcc tcgatcatca tcgtagtgaa
     8221 cccgcttgca gccaccctga tcttcgtctc cctcaccggg acaatggatc aggccgcaaa
     8281 actcaagatc gcgcgagagg caagccagtt cgctctggct gttctgctta tattcacctt
     8341 tggaggcggc tggatactcc agatcttcgg tatatccatc gaggctttcc gcatagcagg
     8401 cggcgtcctt ctatttggta taggtatgga catggtctat gcaaagacat cccggacaaa
     8461 gatgaccgca accgagaagt atgaagggca ggatacagat gacattgcca tcatgccgct
     8521 tgcaatcccg atgattgccg ggcccggggc gatcacctct gtcatcgtgc tgatgaacga
     8581 ggcgatgccg atgggattag ccgcaattgc tatggtgccg ctcgcggtta ttgtagccat
     8641 cgggatcacc tactacatga tgaagaacgc cgatgtcatt gtccggcgca tcggtcagcg
     8701 tgagtatcgt gccgttaacc gcctgatggg gatgctcctc atcgcgattg ccgtccagtt
     8761 catcatagtt ggcatcaaag ccgccttccc cctcctctca ggaggttccg tatgagatct
     8821 aatcttgctc agatcggggc aatccttgcc agcacctgca tcatcgccag cgggattctt
     8881 gttgcagttc agatgtttgc gatcacggtc ccgtttgctc cctacatcat ggtggcactc
     8941 tcgatcctca tcgtcatcgg cgctgccatg ctgattcact cctccaggag cctggagacg
     9001 agtctctcct gatgatgaca gcgatagcgc ttgcacaggt tgccaccggc gcccggcgcg
     9061 gtcgggtggg tgtgctcgat gcagctgcca ggatggcgga tgaggccgcg gggagagggg
     9121 catcgctcat ctgctttccc gagcagttcg tgaccggttg gtcaccggac gtagcgccgg
     9181 gggcgggtga gccccttgac ggtccgcttg caacggcatt tagccatata gcagagcggt
     9241 gcgggatcgc ggtcgcgggg tcgatcatag agtccagtgt gggatgccgc cctaaaaaca
     9301 ccgttgtggt aaccggcgca gacgccaaac tcctggcagc atacgcgaag atccacctct
     9361 tctcgccgga tggagaggac ctggcctacg aagcggggga caggatcgcc acgtttaccc
     9421 ttgatggtac ggtctttggg cttgctgtct gctacgacct gcggttcccg gaactctttc
     9481 ggatctatgc tcttgcgggg gcggagtgtg tcctggtgcc cgcggcatgg ccgtgctcaa
     9541 ggctctcaca ctgggagacc ctcctccccg ccagagctct tgagaaccgt tactacgttg
     9601 ccggggtcaa cacgcccggc atgccgggcg gcgcctgctg cggtggatcg cttgccgccg
     9661 accctgacgg cgtcataatc ggcaggtgcg gtgccggtga agaggtcctg atcgttgaga
     9721 ttgatcccgc ggcggtccat gacgcccgat ccaggctccc ttcactttca gaccgcagaa
     9781 gtgaacttta tcagcggttg ctctccagat tgtagagata tctcatgcgg ggtgaccagc
     9841 acgtatcgct cagtcttaca acggcagcgc ttcttatcgc tcccaatctc agcacaatcg
     9901 accctttcac ggccgtcgtc ctccttttcg gcacatttgt cggatcggtt gctccggacg
     9961 ctgatgccgc cgatgcagcg atattcaacg gtcgtatcag cggggcgaaa ggaaaacgag
    10021 gccaggtaat aaacggcctt gcagtggtgc ttccgatctt tggctatacc atcaggtacc
    10081 tcatctacta cccgatctcg ctggtcttca cactcctcct ccggaagaac taccgccacc
    10141 gccaccgtgg actgcttcac tcgctcccgg gtgtcggcct gaccacgctc atcctctccg
    10201 cgtacctggc cgtgctcctg gcctggctcg ggttatccct cgcgctcctt ccagcattcg
    10261 gctgcgggtt ctttggtggg tctgtccttc acctgctgga ggatgcctgc accccttccg
    10321 gcgtcgcctg gctctacccc ctctcccgcc ggcgggtctc cgggcgtgtc agggcgcaga
    10381 ggttctttga ggtgcgcccg acaatctttg ccgccgttct tgcgacagcg gcggcagggg
    10441 tgctaatcgc gccgttcgtg accgatctga caacggatga actccggttc atcgcgtttg
    10501 cagctgcctt aaccctctgg ctgctgttcc tgctcgtctc cggtgtaagg cgcgaacgac
    10561 gctgtgggtg aggtgaaacc atatctatac gtagatacgc gaacaacaat cttgcatgta
    10621 ccgtctcgtc tgcgttcatt gcggcgcagc ctacccgcag gaccagatca tctacacgtg
    10681 cagccgctgc gggcacctgc tgagcgtcga gtacgacctc gatgatatcg aagtctcgcg
    10741 gggcgagtgg aaccgccgcc ccctatcagt atggcgctac cgcgagattc ttcctgtcag
    10801 cggtgaaccg gtcaccctcc aggagggagg cacacctctc taccatctga agaaaatcgg
    10861 aaaggatctg ggtcttcctg aactctatgc caaacacgag gggatgaacc cgaccggttc
    10921 gttcaaggac cgcggcatga ccgttggtgt cagtatggcg cttgaactcg gtatgaagag
    10981 cgttgcctgt gcaagcaccg ggaacacctc cgcaagcctc gccgcctatg ctgcaaaggc
    11041 cggtgttccc tgtgttgtcc ttctgcctgc cgggaaggta gcccttggca aggtcgccca
    11101 ggcgctgatg cacggcgctc gtgtcatctc catacgcggc aacttcgatc gggcgcttga
    11161 gatggtgcat gaactctgcg tcagccacgg cctctacctc ttaaactcgg tcaaccccta
    11221 ccgcctggag gggcagaaga cgatcgggtt tgaggtgatc gaccaggttg gcggggtgcc
    11281 ggacaggatg gttctcccgg tcgggaacgc cggtaacata tccgccgtct acaaagggct
    11341 caaagagttt gaggctcttg gattcataga ccggttgccc atgatgaccg gtatccaggc
    11401 ggccggttca cagccggttg tgagggcgat cgaacagaac ctcaatgttc tgatcccgga
    11461 atccaatcct gagactgtag ccaccgcgat caggatcggc gcccctgtaa acgcggagaa
    11521 ggcgcttgtt gccatccgcg ccaccggtgg gactgcggcg gccgttaccg atgaggagat
    11581 tcttgcgatg cagaaggacc tggcgcgtaa agaagggatc ggggtcgaac ctgcctcagc
    11641 cgcctcggtt gcgggtgtcc ggagactcgt cgagcagggt ctgatcgata gggatgagag
    11701 gatcgtctgt gtggtgaccg gtcatctact gaaagatcca gaaacggttg tacgacaatg
    11761 cgaacctcca gtcgagatcg acgcggacat atcatcgctg ctctctgtct tgcgctgatc
    11821 cttgtcagcg cacccgcggc agcgcaggag gccgtcttcc aggttctgcc ggatggcacc
    11881 gcctatgaag cctcaataga ggtctcgggg gacacctaca ccctctggac gccggggctc
    11941 ctgggtgagc gcgtccccct ccaggtcgag ggccttgagg tgctcggccc gaagggcgcg
    12001 gttgagtacc gtgaggaagg acggggcgtc atcacctttc ccgaggggaa ctacaccatc
    12061 tcatacaggg ttccggtaag gaacaatcag tttgttgctg cttttgatac gccgtataac
    12121 gtgactgttc tccttcctcc ggtttttaag gtagacaacc cgctcatagg tatggtcagc
    12181 accggcggag tggtatcgcc gggtccgaac gaaaccacgg aaattgcgtg ggacagggcg
    12241 aaggtcgtgg aggtcaggtt ctacacccct gaacgcgaga tgttgctaac caccttcggc
    12301 accgtatggc ttgcggtcgc gcttgtcctg atcttcccgt tcctcttctc gcgcaggagg
    12361 ggcggcaggt gatcgaggtt ttagccgcat ccttcctgat cgggttctcg ggggccgcat
    12421 ccccgggccc gctgaccgct tcggttcttg gcatcggttc gcggcagccg ctgcgtttcg
    12481 tcacggggct tgttgcaggg cacggcgttc ctgaagcggt catggtcgct gccatagcct
    12541 gcggtgtgcg agacgttccg cacatcgatc ttgtcgctct ccttggttca tgcgttctta
    12601 tcgcgctggg cgcaatgcag ttcctgcgtg ctggagatac cctcgttgtg agcgaggaga
    12661 cccggatgcc ggttgccttc gggctggcct gcaccctggg caacccctac tggtgggtct
    12721 ggtggctcac cttcggcgtc gggtttctcg cgctccaccc atcgtttgtt gcgttctata
    12781 tcgggcacat aggcgcggat attgtctggc tcgggctgct tgccgttgca gtatcccggg
    12841 gggcaaacgt cctgggccgc cactacaaaa aagtggtgca ggcgagtgga ctcgccatga
    12901 tgctctttgg tctctacttt attctctcgg ttctttcgac ctgatatcga tcccgaaccg
    12961 ctcggcgttt gcatctgcga tcctctggat ctcggcaatc aggtcatccc gccggctggg
    13021 tttgaagagg tgccggaacc gcttctgcct ggagagatac tcctcgacgg gtttacgttt
    13081 caccttcttt gccttctcca ccacgccgtt ggccatttcg tagttcaccc atagtccggt
    13141 ctcaatggcc agtttggcca tggtgatcgt ctcgcctgac tcaaaacccc atccggtgca
    13201 gcaaggggtg tgcacctgaa tgtagcaggg gcccggtgta ttgatagccc gccggacctt
    13261 cttttcgaag tcggccggat atgcgatcga ggcggtcgcg acgtagggtg caccgtgggc
    13321 cgccaggatc gcgggcatat ccttcttttg gcggacgttg cccggtgagc acgcacccgc
    13381 cggacttgtc gtggtgcttg cggcatacgg cgttgcgcca gaacgctgga tgcccgtgtt
    13441 catgtaggcc tcgttgtcgt agcagacgta ggtgatgtcg tggccccgct cgaaagcgcc
    13501 gctgatgcag agcataccga tatcgaacgt ggcaccgtca ccgcagatac agactacctt
    13561 ctcgctgcgc ccctgcttct tcagcgacgc ctcaataccg gatgcaaccg ccgcagcgtt
    13621 ctcaaagagc gagtggatcc agggaacccc ccaggtggtc tcggggtagg gtgttgagaa
    13681 gacctccatg cacccggtcg agttgacaac gatcacgttc tctccggtcg ccttcaggag
    13741 cagccttgct gcaagcgccg ggccgcaccc gccgcacgcc cggtgtccgg gggtgaagag
    13801 gtcgcaccct tccggtaatc cggtcattca gagcacctcc gtccgcagtc cataaaacat
    13861 atcccctctc ccctcctctg cgagatcgac tatcgcggcg atatccttct tgcgtatatc
    13921 acgcccgccc agagcgacga tgtagtcgta tacggccgtg ccggaaccag agagagctgc
    13981 tttcacctcg gtgccaacac ctccgccgct cccgagtgag atgttcctgt caagcaccgc
    14041 cacggtcgag acccctttga gcgcgtccac gatctcctgc gccgggaaag gccggaacat
    14101 ccggatcttt aagagtcctg cattctttcc ggcatcgcgc atctcatcga cggcatcctt
    14161 tgcggtgccg catatggagc ccatggcaac aatcgcggtg tcggcatcct ccagccggta
    14221 gccctcgacg ggcgcggagt agtctctatc aaactgtttt gcgaactcgt gtccggcttt
    14281 tgcgaagacc tcctttgcac gatgctgtgc acgatcgatc tcgtagcgga actccatgta
    14341 gtaatctgga gtcgcgtaca tcccgaaact cagcggatta ctggcatcaa gccgctggta
    14401 tgggttaaac gcgggcaggt aggcgtccac ctcctcctgc gtcgggatgg agaccggttc
    14461 gtaggtgtga gtgaggacgt agccgtcgaa acagacaaac gccggcagga ggacatcgtg
    14521 gtcttcacag acccgatatg cgatcatgtg gaggtcgacg gcctcctggt tgtcctctgc
    14581 gtagacctgc agccatccgg agtcgcgcag cgatatcgag tcctgctggt cgttccagat
    14641 cgagagcggt gcgccgagcg agcggtttgc gatcgtcatc acgaccggct ggcgcagtcc
    14701 agcaacgttg aagcagacct caaacatcag ggcaagcccc tgggatgtcg ttgccgagta
    14761 gacccgggaa cctgccgcac ttgcaccgag acaggctgag agggccgaga actcgctctc
    14821 gacggtgatg tagtcggcat cgagttcgca gtccgccacc atctgggcaa gggcttcgac
    14881 gatatgcgtc tgcggtgtga tgggatatgc tgcgatcacc tgcgggcggc agcgcttcac
    14941 aatctcggcc accgcgtgcg atccttccat aatctccagc attatttctc ctccttctcc
    15001 atcctgatgg actttttcgg gcagacctct tcacatatgc cgcaaccctt gcagtagtcg
    15061 aggtccggct cgaacatccc ctcatcagtc tcgcgtatac agccttcggg acagaactgg
    15121 gcgcatatgc cgcaccggtt gcaggtctca gggtcaaaga ccggtttgaa gacccgccag
    15181 gtgcccgtct tgttctcaag cgctttgccc ggaggcgcgg cgcaaccaac ccggagcgcc
    15241 atctacgcag cacccccgac cagtttgtat gcgcgctctg ccgcccggat gttcttctcg
    15301 gccaggctcc cggcgaaccg gctccgcagc gcgcctttga gtggttcaag cccgatctcg
    15361 ccggtggctg cagcgaaagc gcccatcagg gcagtgttgg tgatgggcag gccgagttct
    15421 tcgagtgcga tggtggtggc atctatcgtg tagacggtta caccctccgg aacgcggaaa
    15481 tcactggctg tcttctcggt gttgatgata gcgattccac ccggttttat gccattgaag
    15541 acgtccacat cccggatcag ggtggagtcc tgcacaatga tgtaatcggg ctcgtagacc
    15601 tggctacgca gccggatctt tctatcactg aacctgacaa acgcctgcac cggggcaccc
    15661 cggcgttcca cgccaaaagc aggaaacgcc tgggagaaca cccccccctc aaacgcagcg
    15721 caggcgatga gttcggcggc ggtcacggaa ccctggccgc ccctgccgtg aatgcgtaac
    15781 tctctcaatc aataaccccc attaaatctt aatgatcaat aatataaaaa tcaggcactc
    15841 ccggaggggc gcacgcggag catcaatcac tccgggagat ggatgcatcc tgccggtaaa
    15901 ggagattgtg cagggcgctc actggacgtt tgcaagccgg cttttgaggt cttcaggggt
    15961 gcactggcgt atctccataa gggctgagac aacggcgtcg gaaaagacca gcgcagcggt
    16021 ctcaaagagt gtcccgagag gggcaaacga tgcggagacc gaacggtact gaccggtgag
    16081 ctggcggatc tcaaagtcgc ttggaagccc ctggtctttg agctggttgt tcccgatctc
    16141 tacaatgcag tcggcgatac ggcctatgct cgagtcgggt gttgaggtga taagacagac
    16201 tgcccccccg agttctttgg cggtctcgca ggcatcaacg accgaacggg tctcgcccga
    16261 acccgagacc gcgatgagca agtcgcccga tctaaatgcc ggggtgatcg tctcgccgat
    16321 gacatagctc tcaaacccca gatgcataag tcgctgggcg aatgctcgtg ctacaagccc
    16381 ggaacggccg gcgccggcga gatagatccg ttttgcgttc aggatcttat cgaggagctg
    16441 gttcgcgctc tcccggtcta tgttgcgcgc cacttcttcg aggctcgatg tcatctgaag
    16501 catgaaatca gtgatatcag gaaattcctg tggcatggtc gctgtctccc acctgtagtc
    16561 tctatacagc atggcgcagt taatagattt ccctgcctgg aaaaagttgt ttcgtatccc
    16621 gggctatcaa acattaccgc cgggctgtac cctggtttga tgaacctatc cccggatgga
    16681 ttccggtgga tgttcatcgc cgcgggcggc gctctccgct gtcagcatgc tcccttggtc
    16741 tgtgattctc catcgataca gtgtccggga agagcatctc cagcgttcac ggtgagaccc
    16801 ccaaaagatc cggtctccgc ccatggcacg gccgccgggc caatcgccac gcactgctcg
    16861 ccccctctgc aagcgccggg aggcagcgga aggcgagtgg tttcgagaga tgcctgagat
    16921 gtttgatgac ctctctatat gcagggagat cggtgtggcc ctctcaagcc agaggctacc
    16981 gaccggttag ggcaagatcg ccccactcag cgctccaccg caagcccgag gatcccggtc
    17041 gctccggaga agacgtcgcg ggcgatccgg acgaggccgc gcgccgcaca ccactcgtcg
    17101 tagacggcga cctttcggga gaggagcgcc tccacctcag cggcgatggc aggggccatg
    17161 ccccccgcga gggcgacctt cgcatcgggg ttgaggagga gcatcgcagc acactccatc
    17221 gcggcaaaca tcgccatcgt ctccagcctg aggtcggcgt ccatcgtgta gtccacaccg
    17281 gcgtggagga aggcgtcgtt cgccgttatc tccccccggt cgatccggcg tatggcgttc
    17341 acgtcaagag ccccgtgctc cgtccctggg gcgaagatac aggcgtcgaa cgcgccggtg
    17401 atcctgccac ctgtcaccag gagggtgacg gtgtttgaac tcgcgtcgca gaccacgacg
    17461 tcgtctccaa ggtcgtgcac gacgtggtag gcgatcccga tcttctccgg gcttgcctga
    17521 tgcgagtagg ccttgaaccg cgggtcggtc ggtgatccgc ggtgcagccc cgggatgacg
    17581 atggcgggaa gaccgctctc cctgatcgca tcgtagaccc tggtaccgcc gccgatgtgt
    17641 tttccggcac cctcctgtga gaccacaccg cggttctcga ccctcctgat atcggtgatg
    17701 gccgttatcc cgtcacccat cgagtagcag acggcaactc cctcgatctc gtcgagtggg
    17761 cagaggcggg caaggtcgcc cgccgagaaa tacctggcct cttcacggga gatcttgaac
    17821 tccgcctcgc cgcatgagaa gcgcatcgcg gtggtgccgt ggtcgatacc gatgaacatt
    17881 aaatatcacc tttgtatctc aatgggatag taagtttctg gatcgcgcgg tgcgagaggc
    17941 gaggaactca ggctgtgttg gcctggtagc gcctcttcgc gtactccacc gcgccgacaa
    18001 cgacgagacc cgcaaggaag aagatcaggc ctcctggtaa catctccccg gctgccatcc
    18061 ctctctccca gagcatccgg gccgaaccga gcatcagacc ggtgagcagg gcgagcatta
    18121 acccgtgata tgtcgaaagg agatacttga gggcctttac aaagagaagg agacccgtga
    18181 caccgccagc gatgtaggcg atgatctcag ggagtaaaaa tgccctgagc gccgcgagca
    18241 tgaactcgta ctggttcagg acaagggtga tgtatgctcc cgatatcccg ggcaggatca
    18301 tcgcgcatag cgctgccatc cccgtgaaga aaaggatcgg cagcgagtgc ccgatatcca
    18361 tgtggccaag accgccaatg agatagccga caactgcccc gatgaagagg aaggcaacgg
    18421 ttgcatggct atgggagcgg atatcgagga atatggcgat cgcggaggca aatataagcc
    18481 cgaggaagaa ggagtaggtc tcgactgcat aatcggccag aagcgtgagg ataaccccgg
    18541 acatcaggag gaaagcggtg cttatcccgg cgaggaggac gatgaggaac ggtatatcga
    18601 tcctctcgag atccgcccgc agcgctacgg tgtcgcccct cagcaggtct tttgccaggt
    18661 tcaggtcgat gctccctatg gccccgacca gtcgctcgta gatcccggtg atcagggcga
    18721 ttgtcccccc tgagacgccg gggattatgt cgcaggcccc catcatgaga ccgcgcaggt
    18781 agatcccggc gtactcccgg agatccggac gcagattcat ggcgcgactc cacgacgatt
    18841 cttgatacga tagtttccag ccgtaagcag gtcaaaaccc tgcaggggca ggagatgagc
    18901 gcgcgggaag cgatcgtctc ccgcatggca agatgacagt ctcaatcgga caactcctgg
    18961 atcatatgtt gaatggagcc tcttacggaa aatatgtgct gttctaacgc aagggcggtg
    19021 gttaggagag gatcgccaga ccacagcgat cctttcgagt tgctcatgtc cgcgaggcca
    19081 tccagtaatc ctatagcatc agagaggtat tactgtacaa tgttctgtat cgtaacgggc
    19141 ggtgccggtt tcataggttc gcacatcgtc gacgctcttg ttgcggcagg ggataaggtg
    19201 ctggtcatcg atgatttcag tgccggtagc gagaagaacc ttgcgcacca ccaggctggc
    19261 ggccaggtca cctctatcag gcagaacctg ctcgacgacg ggtggcagga acggttttgt
    19321 ggggcagacc gggtctacca tatcgcggct gacccagatg tccggcagag cgccgtaaac
    19381 ccggactcgc agatcaggaa caatatcata actactcatc gggttcttga ggcgatgcgg
    19441 gttcatggcg tgcccgaact tgtctttacc tcaacctcga cggtctacgg cgatgccacc
    19501 gtcatcccga caccggagac ctataccccg cttgaaccga tatccgtata cggcgcgacg
    19561 aaacttgcct gtgaggcgct catatcatca tactgccact cgtttggtat gacgtcctgg
    19621 atctaccggt ttgcaaacat catcggcgaa cggagcggtc atggtgtcat accggacttc
    19681 atccggaaac ttcgcgagaa cccgcgcgaa ctcgagatcc ttggcgacgg ccggcagacg
    19741 aagtcctacc ttgaggtcag ggagtgtgtc cgtgctgtgc agttcgggat tgagcattcc
    19801 cacgacccgg tgaacatctt caacatcggt tctgaggact ggatcgacgt cgtaacgatt
    19861 gccgatatag tcacggaaga gatggggctc tccggtgtcc gctatcggtt cacgggaggc
    19921 gcgcgtggct gggtcggcga tgtgccaagg atgcagctct cggttgagaa actcaagaac
    19981 ctcggctggc ggtgccggac gacctcggaa gagagcgtgc gcagcgccgt ccgcgccatg
    20041 ctgggataaa gtggtgcggt gcagggattt tccatgaaat acatcgtcat tctcggagat
    20101 ggcatggccg atgagccgct tgccgaactg gatgggagga caccccttga gtacgcagag
    20161 acgccgaaca tggacaggat cgcgcgcgaa ggtcgttgcg ggatgctccg gaccgtgccg
    20221 gaggggtttg aacccgggag cgatattgca aacctctctg tcctgggcta cgacccgcgc
    20281 gtctcctata ccggtagggg gccgcttgag gcggccagca tgggcatcga gctcggagag
    20341 ggcgagatgg cataccgctg caacctggtc acggtcaggg acgggttgat ggtggacttc
    20401 aacgccggcc acatatcgag cgccgagggg gcggccctgc tgcgcgatct ggatgccgtg
    20461 ctcgggggtg tccgggtata cccgggtatc agttaccgca acctccttgt cctcccggag
    20521 gggcgcggtt ccctcaccac ccctccacat gacatcgtcg gccgccccat acaggagtat
    20581 ctcccgcgcg gcgatgatgc tgctgcactc cttgagtgca tggagcggtc atgcgaggtc
    20641 tttgccgacc accccgtcaa ccgccgccgc gtgctggagg ggaagatccc ggcgaccggg
    20701 atctggccct ggagcggtgg aaagagaccg tcgcttgagc ctttccgcga gaagtacggc
    20761 cttgccggcg gggtgatctc ggctgtcgat ctcctgggcg gcatcgcccg ccttgccggg
    20821 atggaggtga tccgtgttcc tggcgccaca gggttcatcg ataccgatta caaggcgaag
    20881 gcccgatatg cggtcgacgc cctcgatcgg ctggactttg tctacatgca cgtcgaggcc
    20941 ccggacgagg cggggcacat gggaagtgtc gaggagaagg tgcgggcgat cgagcggctc
    21001 gatgaagcga tcgggatcat ccttgagcgc ccggagacgg tggtcgcggt tctcccggat
    21061 caccctacac cgatacgctg caggacgcat accgctgacc cggtaccttt tgctgtgctc
    21121 gggaagggga aggatggcgt caagggtttc tccgaacggg aggcacggaa aggttcgttc
    21181 gggctcatgg aggcggctgc gtttcttccg atgctcttct cgccgtaaac ctctttctgc
    21241 atgggcaggg aacctgtgag ttcaaccatc tgggataatg gcccccgctc ctgtctcttg
    21301 catgattccg gaaacagcat ctcacaaaaa ataccccagc caccccggga cacaggggga
    21361 aggcggtctg cggcgatgcg gatacctccc ggaagccgtt ccaggcgcgg ctcatcaaat
    21421 taagggccga gtctggagga accggtttga tggcctccgc ggtcacgggt gttgactggc
    21481 cgccaccttt ttgtgctctc catattaaat gcctacactg atatggaacc cct
//
LOCUS       DAOSZX010000017        21523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_1996_length_21523_cov_20.061207, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000017 DAOSZX010000000
VERSION     DAOSZX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21523
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1996_length_21523_cov_20.061207"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..112)
                     /locus_tag="PK196_02695"
     tRNA            complement(join(2..37,75..112))
                     /locus_tag="PK196_02695"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Met,seq:cat)
     gene            complement(185..1660)
                     /locus_tag="PK196_02700"
     CDS             complement(185..1660)
                     /locus_tag="PK196_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HON40367.1"
                     /translation="MKYFDKILIANRGEIAIRIMRACRELGIETVAIYSEPDRNALHV
                     QYADEAFCVGEAHPSKSYLNKERICNVAKKSGAEAIHPGYGFLAENAGFAKLVEEEGL
                     TFIGPSWKTIEILGSKIESKRMMREAGVPVLPGTQEGVTSIDEAKTVASEIGYPVIVK
                     ASAGGGGIGMHIVENEDQLEEAIEKGRRIAESAFGDPTIFVEKYLTKPRHLEIQILAD
                     GAGHIVHLYDRECSIQRRHQKLLEEAPSPIMTPELRERMAESAITVAKTANYKNAGTV
                     EFLYSNGNYYFMEVNTRLQVEHTVTEFITGIDMVKQQIAIAAGYDLTMGQEDISVRGH
                     AIECRINAEDPLNNFAADPGKIVRYRSPGGPGIRVDSGIHVGYTIPAHYDSMIAKLCA
                     WGMNREEAIQRMRRAIYEYVIIGVKTTLPLHYAIMQNPYFIAGETHTHFLQEEHIAAS
                     LRRYLREEEARMQTLAASLRQGKQVAAITAAVDVYIRRNTR"
     gene            complement(1675..3435)
                     /gene="oadA"
                     /locus_tag="PK196_02705"
     CDS             complement(1675..3435)
                     /gene="oadA"
                     /locus_tag="PK196_02705"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HON40368.1"
                     /translation="MCAASLGPLNITDTTLRDAHQSLIATRLRTEDMLPLASAIDDVG
                     FFSVEAWGGATFDTCIRFLDDDPWERLRALKAELKRTPIQMLLRGQNLVGYRHYPDDV
                     VEKFVEAAARNGVDIFRVFDALNDIRNMRKAMEEVRNVGAHLQGAISYTTSPVHSVEG
                     FIGMAEELYSLGCDSICIKDMAGLIMPHDARDLITGIKRVADVKVCLHSHCTSGVAPM
                     SYQTAIDAGVDILDTAMSPFALGTSQPPTESVVASVAGTPRDTGIDLFKLRKVRNICR
                     EIREKYEPLFNPISERVDSDVLIYQLPGGMISNLVSQLKEQDALDRLEEVLREIPRVR
                     EDLGYPPLVTPTSQIVGTQAVFNVLMGGERYQNVTREVKDYVLGFYGRPPAPIRPEIR
                     RRIIGDEEPITVRPADLLEPIYEKMKKEAMEQGFVSREEDVLTYILYPNIAPSFLRGE
                     RQPEVIPDKVTQVAPMGVVEIPHSMEVEVDGEIFSVRILTVDGGAVEPQAAGTEQPVR
                     GAPPRGDLPGAVKSNMQGMVLKVMVERGSTVQKGDTLIVLEAMKMENPIPSPRDGRVT
                     EIFVDVGDVVQNGDVLMVIE"
     gene            complement(3491..4141)
                     /locus_tag="PK196_02710"
     CDS             complement(3491..4141)
                     /locus_tag="PK196_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HON40369.1"
                     /translation="MQDPRLRLFTLVVLSLASFFSVAGALGAFIWWLAATPRTKALPR
                     PRVLLPLFAMIALTALVSALGGGDGLSYLLRMSVILLLGAWAYAGTEEGEVLSVAVWA
                     LGNRKGFDIGLIAEMGLAGLGVIREEIEQVAVAMRLKGIRPGLRSIVPLAIILIITEI
                     RRTDDVARLLTVRGYTAGGVICPRFQRGSNDILASISAVLLGILPTLFLRDVFILL"
     gene            complement(4123..4821)
                     /locus_tag="PK196_02715"
     CDS             complement(4123..4821)
                     /locus_tag="PK196_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HON40370.1"
                     /translation="MRVELEEVLFSRGAFCLRASGAFDEGVHLVSGPVGSGKSTLAVL
                     LAGLMPPASGVVRWQGVNSVLLLLQFPEHQITSPTVAKEAASWGLVPEEVLALADLEE
                     RADDDPFNLSRGELKRLTLACAFIRDPDLLLLDEPFGSLDCERKGWVCGMIEERGSGV
                     TIIFSHEHAVLPRVDAIWEMDGGALRGCGSVPGAIPSWRHAPAYLTYALEMGARPENI
                     RLADAREALCRIRD"
     gene            complement(4818..5459)
                     /locus_tag="PK196_02720"
     CDS             complement(4818..5459)
                     /locus_tag="PK196_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HON40371.1"
                     /translation="MLEIPDLELDARHIAVIGPNGSGKTTLLEVISGIDEPETGTIRI
                     LGRPPSSLRVGWVGEFPDRTLLFSRVYDEIASTPRFRHFPCPETDRLVLAAAGLVGIS
                     HLLERKVASLSGGEKALVALAAACVGDPEVLVLDEADSHLDAGAAERLKKVVQESPAT
                     HILWCTQSMDTAATADCIIFMKGGCILYQGTPEEVFDALRETCYYPTIWRLRG"
     assembly_gap    5875..5974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5988..6494)
                     /locus_tag="PK196_02725"
     CDS             complement(5988..6494)
                     /locus_tag="PK196_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HON40372.1"
                     /translation="MIQRARILALSATFIALIAVGSWLSIPLPPVPITLQTLFVLLAG
                     IVMKRYGVIPVALYILLGAIGLPVYHNGTAGPGILLGPTGGFLVGFIPAALVVGLVYE
                     HKSPKLRMAGLFAATAVTYVFGVAWLSFSASLSIYQAILIGVAPFVVGDIVKIAAAYT
                     IGKRVETG"
     gene            complement(6671..7654)
                     /locus_tag="PK196_02730"
     CDS             complement(6671..7654)
                     /locus_tag="PK196_02730"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HON40373.1"
                     /translation="MNETAIKVLKILEESPGPIPGEQIAEQLGISVSNVWKQIRELRR
                     LGYRISSSRGEGYRLEARTDRLLPYELNRKLRTRVIGRQIRHFERTASTSWVAKRLIP
                     ETDPEKLHGMVIIAEEQTGGVGRLGRAWVSPAGGIWATIVLKPKIPLDHLFMITMAGS
                     IAIARAIRKEYNLSALIKWPNDIFIGDKKVAGLLLEISAEADTVHYALLGMGIDANIS
                     LDELTPPLRETVTTLEAEVGHPVDRVSLFARILHEFELRYQQLEDGEYESIIHEWKGL
                     SLTLERRVSIRTINKTFSGEAIDIDEHGALIVRKDNGKVERVIAGDCFLQI"
     gene            complement(7660..8094)
                     /locus_tag="PK196_02735"
     CDS             complement(7660..8094)
                     /locus_tag="PK196_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HON40374.1"
                     /translation="MDTVMEQYIISASSGARDLAPLATCVHDLLNRLPVTARSRDRPG
                     VRLEEGKVIDTAYTGPVLEAVLAENVTKRVRSPSGPYRGIPVVVAPIRDEKGSAIAAI
                     GIVDVTGVFDLADFMEQASAVRREVCGEDPCPLPTESPAAKR"
     gene            complement(8114..8692)
                     /locus_tag="PK196_02740"
     CDS             complement(8114..8692)
                     /locus_tag="PK196_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40375.1"
                     /translation="MTCRVGLLWDTPLVFSRLIEDCGASCEGITPNMLAAPFWRGRFV
                     SLIVPTGFADPAYSKLLPALRASSGRIERFVEKGGRLLVFGAASPRQDAYDWLPFPVT
                     YAFSYGRRAVRFTMDSPYNTLLSGYDLSAIECDGTFPSHCGETLAATPDGEPLLIMKE
                     IGDGVILVTSVHEYPSREFLAEFSCADRETLF"
     gene            complement(8689..10125)
                     /locus_tag="PK196_02745"
     CDS             complement(8689..10125)
                     /locus_tag="PK196_02745"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HON40376.1"
                     /translation="MLAGINRVGDLEWEVPIGYVPGMRVPGRFFLSEALSETLEEGAV
                     RQVANVATMPGIVKHSLAMPDIHWGYGFPIGGVAAFDMTEGVISPGGVGFDINCGVRL
                     ITTPLAEADIAGRKRELIERLFGAIPTGVGAKSALRVSNKDLNTIMVEGARWAVERGF
                     GTEADLVRCEAEGAMPGADPDAVSSKARQRGVPQMGTLGAGNHFLEVQVVREILDPAA
                     AEVFGLKEGQVCFMVHCGSRGLGHQVATDHLRTLEAGLTKYRIRLPDRQLACAPIDSP
                     EGRGYYGGMVCAANYAWANRQVIMHEARNVFAKLFGIEYDGMRLVYDVAHNVAKFERH
                     EVDGKLTEVCVHRKGATRAFGPKSPEIPHEYTAIGQPVIIPGSMGTSSYLLRGTATAM
                     QKTWGSTCHGAGRVLSRSKVKKEVRGKDVREHLAGEGILVRAHSDAAIAEEAPEAYKP
                     SHEVVRVVHEVGLSAIVARLEPLGVIKG"
     gene            complement(10140..10547)
                     /locus_tag="PK196_02750"
     CDS             complement(10140..10547)
                     /locus_tag="PK196_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HON40377.1"
                     /translation="MGFEELEHTADLRLRVRGATLDEVFAEAGRAIFREMYGPCKDGG
                     VEQTIELEAENLEALLFDYLSELLFITDVENVVFCTFDVNIQGTRLTAVLRGEPFDPD
                     RHAGGTLIKGVSYSGLEIRRDKDGYVVEIIFDV"
     gene            complement(10554..10820)
                     /locus_tag="PK196_02755"
     CDS             complement(10554..10820)
                     /locus_tag="PK196_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40378.1"
                     /translation="METDIKEPGQSQGIYITEDVRSYILQRKRDFRVSTSCGGPILLP
                     VSVKPPKATDLQIPVGDYTVYVSKYQARYLDCIHRGMIPFFYDG"
     gene            complement(10883..11197)
                     /locus_tag="PK196_02760"
     CDS             complement(10883..11197)
                     /locus_tag="PK196_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HON40379.1"
                     /translation="MRSPGELKKEIEDRLKSYLSRDRDGLRHEVLGLFVKVRTLTIPQ
                     IYETLRMQFAITYHSIASMVGIIASRIGILHVRRNAEGTNSVYELKEQYVDLVAKILG
                     TT"
     gene            11741..12595
                     /locus_tag="PK196_02765"
     CDS             11741..12595
                     /locus_tag="PK196_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HON40380.1"
                     /translation="MEFARYDVNRYSPRQMVALPLVILLLALMVLGYTTLTTGLPVTP
                     GIDFAGGTAVTLFTSESKETIEAVFAGYPLLSVGEGIGGGRYIQFGPMDDAEYQSLVA
                     LINERYPDAKIDQIGETFGETLRSQAFLALLFSFIGMTIVVMIAFRNLVPAGAVVLSA
                     FADIAVSAAVMQALGIPLSLGTTAALLMLIGYSVDSDILLTTRLLKRKGKLDEKLAGA
                     FRTGIIMTTTTLAAIAAMWVVATAGQIQIISEIASVLLIGLVVDLMNTWMLNAGILKG
                     YILRGEKR"
     gene            12592..14052
                     /locus_tag="PK196_02770"
     CDS             12592..14052
                     /locus_tag="PK196_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HON40381.1"
                     /translation="MNSKTLKKLLKDWRVALLLLLVVGSLVGIYLIPPNPEKGFEGNL
                     QFGLDLEGGSWLQLEFQSVVVGYSTTGSVDDLVTNLQTALDTEVIRIDENHLEIRKDI
                     QRDDLEPIFAESGASIVTYQKGVSAYTADEVKRILNEKVNALGMQDARIHLLTPTGSG
                     FPQYVRIELAGVDMATAQEIVGKQGVFEIRIQTAGNETQHVLYGDVITSVSVPQKDPY
                     TQEWGVGFTLSDAGAEAFREAALRYGAVDNPSAHHLIMLLDNKTVYSAPLSSDLAAQL
                     RSAPVRSLSATTGTGDAGLDAAMALEIHLRAGALPVKVDIVGSGSVPAALGEQFKTMI
                     VIAGLLALLTVGLVVYYRYREAAIVLPMVAINLSEIIILLGIARFVIQLDLATIAGLI
                     AVMGTGIDQLVIITDEVLHEGRVPSPSLYMKRYERAFGIIALSAATVIIAMLPLALMD
                     LSTLRGFAIITILGVLVGILVTRPAYGKIVMAILSR"
     gene            14112..14399
                     /locus_tag="PK196_02775"
     CDS             14112..14399
                     /locus_tag="PK196_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HON40382.1"
                     /translation="MGKPVLMDFFAEWCGPCHQQSPIIDELRKRMGDRVEIRKIDVGV
                     DSEETRRYAMKYDLQFVPTLVIEKDGQIVRKLVGVQDLATLESILKPLVEK"
     gene            14396..15046
                     /gene="npdG"
                     /locus_tag="PK196_02780"
     CDS             14396..15046
                     /gene="npdG"
                     /locus_tag="PK196_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HON40383.1"
                     /translation="MKIGIVGGTGEIGEGMALRLSPRYDVIVGSREEAKAVMTCEACR
                     ETLEGQGISCSLVGASNQAAVDGADLVVLAIPFKHVAPTLKTLGGFEGKIVISPVNPI
                     ERETYFYYAPPPEGSAAMMIRGMLPATATVCAAFNNVAANRWKKIDEELDYSVAVCCD
                     DPAAKQTVMEMVNNVSRLRAYDAGPLAAASMVESVTPLLLNIARFNRMRDVGVRFI"
     assembly_gap    15063..15162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15163..15534
                     /locus_tag="PK196_02785"
     CDS             <15163..15534
                     /locus_tag="PK196_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HON40384.1"
                     /translation="APPEASSEYRVHRAVYRTTPHRAIVHAHPVHAVAASLDLDLIRP
                     VDSEGRMLCPEIPVVTGEPGTDEIARNVAEALISGRIAIARGHGTFAAGETLDEAYIY
                     TSIAEYACRILILSGRLRRVA"
     gene            complement(15505..16431)
                     /gene="aglJ"
                     /locus_tag="PK196_02790"
     CDS             complement(15505..16431)
                     /gene="aglJ"
                     /locus_tag="PK196_02790"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HON40385.1"
                     /translation="MSIERDEVCVLIPTLNEAPTIGDLVRGFKERGYEHILVIDGNST
                     DGTPEIARAAGAVVRTQTGKGKGNAILEALEIIDKPRVLMLDGDGTYSPDDAERMLEP
                     LGKGFDHVIGDRLACPEKGAFTRLNLLGNQILNHVFRVAHGKDLHDILSGYRAFTLHS
                     IRQMNLKEAGFEIETEMAVEAVRTGQRVAVVPVRYSARPGTVTKLNPLQDGLRIFSTI
                     YRLAKMNNPIFYFGIIGLIISIIGGVIGIYVVLEWFKNIEHLPLTILTVLLIMMGFQM
                     FMFGVISDMLLGFQRETIRQIEQLRNPPKPPR"
     gene            16473..17066
                     /locus_tag="PK196_02795"
     CDS             16473..17066
                     /locus_tag="PK196_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HON40386.1"
                     /translation="MHIAKPGLRALGVAESYSGREYSTLAGVVMRKDLRIDGVGFARV
                     TVGGMDATDAVIRLYSRLGRRDINFLMIGGNVIAWYNIIDPAAVHEATGLPVIIVTYE
                     ESEGLEEDIRRHFPGDDARLAAYRRLGERMPVRLRSGCTLFIRSCGIAGEDAARLCNG
                     FTYEGRVPEPIRVARLIARGVVRSSGSPGLAPDNHDR"
     gene            complement(17025..17486)
                     /locus_tag="PK196_02800"
     CDS             complement(17025..17486)
                     /locus_tag="PK196_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HON40387.1"
                     /translation="MFRSILVAVDGSDIGQRALEQGLAVASAMEIPVHVVHVIQTAAY
                     PALALSDLPPPDVAQQALLDALDREADEILADAERTAAAAGVPVTIHKQLGHPGAEII
                     ALARKIGAGLIVVGSHGKGRLGRLFLGSTSSYVVDHATSTVMVVRGEPRTS"
     gene            complement(17536..18165)
                     /locus_tag="PK196_02805"
     CDS             complement(17536..18165)
                     /locus_tag="PK196_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HON40388.1"
                     /translation="MISAETWIDTPADSARALPGSTEWINRTRSPRRSAGPSRRTAAS
                     ESGVSETHGVLILVGVTVILAVLVLLMLLAMIPSWSWAEPAQPPIIITGVSHHSAITG
                     KMTYASRVTLLNNGSTVYENDCLKAVFYVNGQKVCIVQTLNGYLFIPSHHYGVKTIGG
                     AGCRTEYWNPGERIDIDLTDTTLYPGAKVCVEIIDKRDGKVISKDAVRA"
     gene            18496..19428
                     /locus_tag="PK196_02810"
     CDS             18496..19428
                     /locus_tag="PK196_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40389.1"
                     /translation="MAEPERLPGYLRLHRSGELEERAERAHEVLRDCVLCPRECHVNR
                     IEDETGFCRTGLLPVVASYNPHFGEEPPLVGRRGSGTIFFSGCSMRCDYCQNFDISQG
                     GIGYAISCDDLAGVMLRLQEMGCHNINLVTPTHVVPQILRAVTMAAGRGLRIPLVYNS
                     GGYDSAETLRLLDGVIDIYMPDAKYGRDDAALALSHAPNYTASMKAALKEMHRQVGDL
                     VIEDGIALRGMIIRHLVLPANLANSEIVMEFIARELSRDSYVNVMAQYRPAGRVLQGR
                     LNPEFAVLRRPITAEEYAYALRCARENGLYRGIF"
     gene            19485..20228
                     /locus_tag="PK196_02815"
     CDS             19485..20228
                     /locus_tag="PK196_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HON40390.1"
                     /translation="MKDKGWLRYAAMTDIGMRERNEDAFFAGRVSGYHLFAIADGLGG
                     HACGDVAGRMAVEILEEAASEELSAFDPAEALKRAFRRINTAIFAFNRERGLNAGTTL
                     SVAIVGDAGRCWIGTVGDSRTYIITPSSVWHTRDHTYVQSLVDAGMISPAEAFYHPAK
                     NILTRALGLEARVEMDLDERELSPDSVLLLSSDGLHDYVPGDLIQQIVMANDPDTACR
                     KLIDAAREAASTDNTTVIVVKPLLGARHP"
     gene            complement(20188..20775)
                     /locus_tag="PK196_02820"
     CDS             complement(20188..20775)
                     /locus_tag="PK196_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON40391.1"
                     /translation="MRSLQRMIDVPIIHRGCETTMTPKNISPGGDRLDSRIKELISML
                     SDPEKGVREEAMRGFVAIGRPAVPACISLLESTDWRVRYRAAETLGLIGGHEAYDPLL
                     SALQDSKDHVRYMAAKGLGLIGDSRAVASLAALQHDENEFVRRSAARSLGQIGGGEAI
                     RALRQALDTESVEAVRAAIVEALRDAGHQAGVSRR"
     gene            complement(20844..20969)
                     /locus_tag="PK196_02825"
     CDS             complement(20844..20969)
                     /locus_tag="PK196_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40392.1"
                     /translation="MYAEENRFASDPIFNALGRVIPGTGIFLSVCLILYLIGNLI"
     gene            complement(21185..21257)
                     /locus_tag="PK196_02830"
     tRNA            complement(21185..21257)
                     /locus_tag="PK196_02830"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21221..21223),aa:Asp,seq:gtc)
     gene            complement(21273..21379)
                     /locus_tag="PK196_02835"
     tRNA            complement(join(21273..21308,21342..21379))
                     /locus_tag="PK196_02835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21343..21345),aa:Tyr,seq:gta)
BASE COUNT         4417 a         6366 c         6144 g         4396 t
ORIGIN      
        1 atgctgcggg tgggatccga acccacgatc tccagatgtc ccaggtctgg acgcaccgtt
       61 tcttcacaaa accctatgag tctggcgccc taaccagcta ggccaccgca gcacacaaag
      121 tgcagtataa caatgctgta ttgacaaata aaccttatgc ctgaccgaaa aatcggatag
      181 agggttacct cgtgtttctc cggatgtaga catcgaccgc tgccgtgatt gctgcgacct
      241 gctttccctg gcgcagagat gcggccagcg tctgcatccg tgcctcctcc tcgcggaggt
      301 aacggcgcag actcgcggct atgtgctcct cctgcaggaa gtgtgtgtgg gtctcgccag
      361 cgataaagta tgggttttgc atgatcgcat agtggagggg gagcgtcgtc ttcacaccga
      421 tgatgacata ctcgtagatc gcccggcgca tccgctggat agcctcctcg cgattcatac
      481 cccatgcaca gagtttggcg atcatcgagt catagtgtgc cgggatggta taccccacgt
      541 gaatgccgga gtcgacccgt atcccgggcc caccaggaga tcgatagcgg acgatctttc
      601 ccggatcagc ggcaaagttg ttcagcgggt cctctgcgtt gatccggcac tcgatcgcgt
      661 gcccccggac gcttatatcc tcctggccca ttgtcaggtc atagcctgcc gcaatcgcga
      721 tctgttgttt caccatatcg atcccggtga taaactccgt cacggtgtgc tccacctgca
      781 gccgggtgtt cacctccatg aagtagtagt taccgttcga atagaggaac tccaccgtgc
      841 cggcgttctt gtagtttgcc gtcttcgcca cggtaattgc cgactccgcc atccgttccc
      901 gcagttcggg ggtcatgatc ggagacggcg cctcctcaag cagtttctgg tgccggcgct
      961 ggattgagca ctcgcgatcg tagaggtgga cgatgtgccc cgccccatca gcaaggatct
     1021 ggatctcaag atggcgcggt tttgtaaggt acttctccac aaagattgtc gggtcgccaa
     1081 acgccgactc tgcgatcctc ctgcccttct cgatcgcctc ctcgagctgg tcctcgttct
     1141 cgacgatatg catgccgata ccgccaccac ccgcgctcgc cttcacgatc accgggtagc
     1201 cgatctccga tgcgaccgtc ttcgcctcat caatgctggt gacaccctct tgggtgccag
     1261 ggagaacagg gacacccgcc tcccgcatca ttcgtttcga ctcgatcttt gagcccagga
     1321 tctcgatcgt cttccaggac gggccgatga acgtcagacc ctcttcctcg accagttttg
     1381 caaacccggc gttctcggcg aggaacccat agccggggtg tatcgcctcc gccccgctct
     1441 tcttcgcaac gttgcagatc cgctccttgt tcaggtagct cttagaaggg tgtgcttccc
     1501 cgacacagaa agcctcatcg gcgtactgga cgtgaagcgc attcctgtca ggttcagagt
     1561 agatcgcaac cgtctcgatg ccaagctccc tgcatgcacg catgatccgg atggcgatct
     1621 cgccgcggtt ggcgataagg atcttgtcaa agtacttcat cgcgcaaaca gccctcactc
     1681 aatgaccatc aaaacatcgc cattctgcac aacgtcccct acatccacaa agatctccgt
     1741 gacccttccg tcgcgggggc tggggatggg attctccatc ttcatcgcct cgagaactat
     1801 gagggtgtct cctttctgga cggtgctccc gcgctcgacc ataaccttaa ggaccatccc
     1861 ctgcatgttg ctcttgacag cgccggggag atcaccgcgg ggtggtgccc ccctgacagg
     1921 ctgctccgtt cctgcagcct ggggttcaac tgcaccccca tccacggtca ggatccggac
     1981 agagaagatc tcgccgtcca cctcgacctc catggagtgc gggatctcca caacccccat
     2041 cggcgcaacc tgcgttacct tatcagggat aacctccggc tggcgctctc cccggaggaa
     2101 cgagggagcg atgttcgggt agaggatgta ggtgagaaca tcctcctccc tggagacaaa
     2161 accctgttcc atcgcctcct tcttcatctt ctcgtagatt ggttcaagca gatccgccgg
     2221 gcgaaccgtt atcggctcct catcaccgat gatccgcctc ctgatctcgg ggcggatcgg
     2281 tgccgggggg cggccgtaga acccaaggac gtagtccttc acctcacggg tcacgttctg
     2341 gtagcgctcc ccgcccatga gaacgttgaa gaccgcctgg gtgcccacaa tctggcttgt
     2401 cggtgtcaca agcggcgggt aaccgagatc ctctcttacc ctcgggatct cgcggagaac
     2461 ctcctccagg cggtcgagcg catcctgctc cttgagctgc gagaccaggt ttgagatcat
     2521 cccgcccggg agctggtaga tcagcacatc cgagtcgaca cgctcggaga tggggttaaa
     2581 gagcggttca tatttctcac gtatctcgcg gcagatgtta cggaccttcc gcagtttgaa
     2641 gagatcgata ccggtatcgc gtggtgtccc ggcaacgctc gcaacgacgc tctccgtcgg
     2701 aggctgggat gtgccaagag caaacggcga catcgctgtg tccaggatat ccacacccgc
     2761 atctatcgcc gtctggtagc tcatcggcgc aacaccgctc gtgcagtgcg agtggaggca
     2821 taccttaacg tccgccaccc tctttatccc ggtgatcaga tcgcgggcat cgtgcggcat
     2881 gatcaggcct gccatatcct tgatgcatat cgagtcacag ccaagcgaat agagttcctc
     2941 cgccatacct atgaaaccct caacggagtg aacagggctt gtcgtgtacg atatcgcacc
     3001 ctggagatgc gccccgacgt tcctgacctc ctccatcgcc ttcctcatat tccggatatc
     3061 gttcagtgca tcaaacaccc ggaatatatc gaccccgttt cgcgccgccg cttcaacaaa
     3121 cttctccacc acatcgtcag gatagtgtcg atagccaacc aggttctggc cgcgcaggag
     3181 catctggata ggggtgcgct tcagttcggc cttcagcgcc cgcaggcgct cccatgggtc
     3241 atcatcgaga aacctgatgc aggtatcaaa agtcgcccca ccccaggcct cgactgagaa
     3301 gaaccccaca tcatcgatgg cgctggcaag agggagcata tcctccgtcc gcagacgggt
     3361 agcgatgagg gactgatggg cgtccctgag cgtggtatcg gtgatattga gtggacctaa
     3421 actggcagca cacatcgaac catacacctc aaagggctga gggatatcaa taaagcctct
     3481 caaaaattca tcataataat ataaaaacgt cgcgaaggaa gagagtgggt agaataccaa
     3541 gcaacaccgc agatatagag gccagaatat cgtttgaacc cctctggaac cgcggacaga
     3601 ttactccgcc cgccgtgtat ccacggacgg tgagcagtcg tgcaacatca tcggttcgtc
     3661 ggatctccgt gattatgagg atgatggcaa gcggcacgat cgaacgaaga cccggccgga
     3721 tacctttcaa tctcatggca accgccacct gctcgatctc ctcccggata accccaagac
     3781 cggcaagccc catctcggcg atgagcccta tatcaaaccc cttccggttc ccgagcgccc
     3841 ataccgccac ggaaagcacc tccccctcct ccgtcccggc atacgcccat gcaccaagga
     3901 gcaggatcac gctcatccgc aggaggtaag acaacccatc gccacccccc agcgcggaga
     3961 cgagcgccgt cagggcgatc attgcaaaga gtgggaggag gacgcggggg cgggggagag
     4021 cctttgtgcg cggtgtcgcg gccagccacc atataaaagc accaagcgct cccgcgacgc
     4081 taaagaacga ggcaagcgag aggacgacga gggtgaatag cctcagtcgc ggatcctgca
     4141 taacgcctca cgtgcatcgg ctagcctgat gttctccggt cgcgcgccca tctccagggc
     4201 gtaggtaagg tatgccggcg cgtgccgcca tgaggggatg gcgccgggca cgctcccgca
     4261 acctctcaga gcgcctccat ccatctccca gatggcatcc accctcggaa gaaccgcatg
     4321 ttcgtgcgag aatatgatcg ttacccctga cccacgctcc tcgatcatac cgcagaccca
     4381 tccctttctc tcgcagtcca gcgaaccgaa gggctcgtcg agcagcaaca ggtcgggatc
     4441 cctgatgaac gcgcaggcca gtgtcaaccg cttgagttca ccacgggaga ggttgaacgg
     4501 atcgtcatcc gcgcgctcct ccaggtccgc gagcgcaagc acctcctccg gcacaagccc
     4561 ccaggaagca gcttcctttg caaccgtcgg ggacgtgatc tgatgttcgg gaaactgcag
     4621 cagaagcaga accgagttga caccctgcca ccggacgacg ccgctggctg gaggcatgag
     4681 accggcaagc aggacggcaa gcgtcgattt tccgctcccc accgggccgc tcacgaggtg
     4741 aacaccctca tcgaaggcgc cgcttgcacg caggcagaaa gcgccacggg agaagagaac
     4801 ctcctcaagt tcgaccctca tccccggagc ctccagattg tcgggtagta gcaggtctcc
     4861 ctgagggcgt caaagacctc ttccggcgtc ccctgataca ggatacagcc acccttcatg
     4921 aaaatgatac agtcagccgt tgccgcggtg tccatcgact gcgtgcacca gaggatgtga
     4981 gtcgccgggc tctcctgcac gaccttcttg aggcgttctg ccgccccggc gtccaggtga
     5041 gagtcggcct cgtcgaggac aagcacctcg ggatcgccca cgcaggccgc agccagcgcg
     5101 accagtgcct tctcgcctcc ggagagcgag gcgaccttcc tttcaaggag gtggctgatg
     5161 ccgaccaacc ctgctgccgc caggaccagc ctgtccgtct ccgggcaggg gaagtggcgg
     5221 aaccttggcg tcgatgcgat ctcatcgtat acgcgggaga agagaagcgt ccggtcgggg
     5281 aactccccga cccatccaac cctcaacgag gatggcggac gacccaggat ccggatcgtc
     5341 ccggtctcag gttcatcgat ccctgaaatg acttctagca gcgttgtctt gccgctcccg
     5401 ttcggcccaa taacggcaat atggcgtgca tccagttcaa ggtcgggaat ctcaagcacc
     5461 tggtgcctga gaccgcttat acggatcatc tgggtggggc agggggactc ccggcttgag
     5521 accggggagg aactgccctc tcgcctccgg taagatcttc tcctggcact attgaaacgt
     5581 tcacgttcac catcgggttc gcgactctca ccggcacgat ccaatttttc cggccgcacc
     5641 ggtgcagtga ggttgcgcgt tcaaggccat gggagctgcc gggtgcgaag gtttgccgga
     5701 acgaagaccg cgagagcgct gtgtgatgca gtcggcggtc atcagcgtga atccggttca
     5761 cgggattgca acggtgagag gagggggcaa agcaacgttc aacaccccgg gttgtcatca
     5821 tcactacctt acctggacta tcaggactat atgttttacg aagaaaacag gccgnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggggta gttgacgcta cccggtttcc
     6001 acccgttttc ctatagtgta tgcggccgcg atcttaacta tgtcaccgac gacgaacggc
     6061 gccaccccga tcaggatcgc ctggtatatc gagagcgacg cggagaatga gagccacgcc
     6121 acaccgaaga cgtaggtaac tgcggtcgcc gcaaagagtc ccgccatcct gagtttcggc
     6181 gatttatgct catagaccag cccgacaaca agagcggcag ggatgaaacc aaccagaaac
     6241 ccgccggtag gcccgaggag tatcccggga cccgctgtgc cgttgtgata gacagggagc
     6301 ccgatcgcac cgaggaggat gtagagggca acggggatca ccccgtagcg tttcatgacg
     6361 atcccggcaa gaagcacgaa gagggtctgc agggtgattg gaaccggagg cagggggatg
     6421 gagagccagc tccccacggc tatcagcgcg atgaacgtgg cactgagggc aagaatcctg
     6481 gcccgctgga tcatgtcagc ctcctcatgg tggtatggtg aaagtgaact accccgcgcc
     6541 tctcgggtgg ggcttcctgc ttcatcctcc ctgaagggtg gggtcttcgc gctccgcccc
     6601 cttcacccca gcaggataaa aatcctgtca acaaaaaaaa acagggggcc ggcagacaga
     6661 gagggtgggg tcagatctgg aggaaacagt cccccgcaat aacccgttcg accttgccgt
     6721 tatcttttct gacgatcagg gcgccatgtt catcgatatc gatggcctcc ccggagaagg
     6781 tcttgttgat tgtcctgatg gacacccggc gctcgagcgt cagggagagg cccttccact
     6841 cgtggatgat ggattcatac tccccgtcct cgagctgctg gtagcgtagt tcaaactcgt
     6901 gcaggatccg ggcaaagagt gagacgcggt caaccgggtg accgacctca gcctcgagag
     6961 ttgtcaccgt ctcccgcagg ggcggcgtaa gttcatcgag agagatgttt gcatctattc
     7021 ccatgccgag gagggcataa tgaacagtat cagcctcagc agaaatctcc aggagcagcc
     7081 ccgcaacctt cttgtcgccg atgaagatat cgtttggcca tttgatgagc gcgctgaggt
     7141 tgtactcctt gcggatggca cgggcgatcg cgatcgaccc ggccatggtg atcatgaaga
     7201 ggtgatcgag cgggatcttc ggtttcagga ctatggtggc ccagatcccg ccggcaggtg
     7261 agacccaggc gcgtccgagc ctcccaaccc cgccggtctg ctcctcggcg atgatcacca
     7321 taccatgcag tttctccgga tcggtctcgg gaataagcct tttcgcgacc cagctagtgg
     7381 atgcagtacg ctcaaagtgg cgaatctgcc tgcctatgac ccgtgtgcgc agtttcctgt
     7441 tgagctcgta tgggaggagc cgatcagtcc tcgcctccag acggtagcct tccccgcgtg
     7501 acgatgatat acggtatccc aaccttcgga gttctcgtat ctgtttccag acattcgaga
     7561 cgctgattcc cagttgctct gcgatctgtt cgcccggtat cggacctggc gactcttcaa
     7621 gaatcttcag gactttgatt gccgtttcgt tcatgtccac tacctttttg ctgcaggtga
     7681 ctcagtcggg agagggcagg gatcctcacc gcatacctcc ctcctgacag ctgaggcctg
     7741 ctccatgaaa tccgccagat cgaagacccc ggtcacatca actataccga ttgcagcaat
     7801 ggcactgcct ttctcatccc ttataggcgc caccactacc gggatccccc tgtaagggcc
     7861 actcggggat ctgacccgtt ttgtgacgtt ctcggcaagc accgcctcga gaacggggcc
     7921 ggtataggct gtgtcgatga cctttccttc ttcaaggcgg acgccgggcc tgtcacggga
     7981 tcgtgccgtg acgggcagcc gattgagcag atcgtggacg cacgtcgcaa gcggtgcaag
     8041 atcgcgtgcg cccgaagacg cggagatgat gtattgttcc atgaccgtat ccataccttt
     8101 catcacggtt gatctaaaat aaggtttccc tatcagcgca cgagaactcc gccaaaaatt
     8161 ctcgtgatgg gtattcatgg acactggtaa ccaggatcac accgtcacct atctccttca
     8221 tgatcaggag gggctctcca tccggcgtgg cggcaagagt ctccccgcaa tgggacggaa
     8281 acgtcccgtc gcactcgatg gcgctcagat cgtagcctga gaggagggtg ttgtaagggc
     8341 tatccatggt aaaccgtact gcacgacgtc cgtaggagaa cgcatacgtc accgggaatg
     8401 ggagccagtc gtaggcgtcc tggcgcggag atgctgcgcc aaagaccagc aatctcccgc
     8461 ctttctcaac gaaacgctcg atccgcccac ttgaagcccg cagtgccggc agtagttttg
     8521 agtatgccgg gtccgcaaac ccggtcggca ctatgagcga gacgaacctg ccgcgccaga
     8581 agggcgctgc gagcatatta ggagtgatcc cctcacagga tgcgccgcag tcctcgatca
     8641 gccgcgagaa gacgagaggg gtgtcccaga gcaacccaac tctgcaggtc atcccttgat
     8701 caccccgagc ggttccagcc tggcgacgat agccgagaga cccacttcat gcaccacacg
     8761 gaccacctcg tgacttggtt tgtaggcttc aggcgcctct tccgcaatgg ccgcatcgct
     8821 gtgcgcacgc accaggattc cttcccctgc cagatgttcc cggacatctt ttccgcgaac
     8881 ctctttcttc accttcgacc ggctgagcac cctgccggcg ccgtggcagg tgctgcccca
     8941 cgtcttctgc atcgctgttg ctgtgccgcg gaggaggtag gaggacgtcc ccatgcttcc
     9001 agggatgatc accggctggc cgatcgcggt gtattcgtgg gggatctccg gcgatttcgg
     9061 cccgaaagcg cgtgttgcac ccttgcggtg tacacagacc tctgtgagtt tgccatcaac
     9121 ctcgtggcgc tcgaacttgg cgacgttgtg ggcgacgtcg tagacgagcc gcatcccgtc
     9181 atactcgatc ccgaacagtt tcgcaaatac gttcctggcc tcgtgcatga tcacctgccg
     9241 gtttgcccag gcgtagtttg cagcgcagac catcccgccg tagtagcccc ggccttccgg
     9301 agaatcgatt ggagcgcagg caagctggcg gtccggcagg cgtatccggt atttggtgag
     9361 accggcctcg agcgttcgga ggtgatcggt tgcgacctgg tggccaagcc cgcgtgaacc
     9421 gcagtgaacc atgaaacaga cctgcccctc tttgagcccg aagacctctg ctgctgcagg
     9481 gtcgagaatc tctcgcacaa cctggacctc gaggaaatgg ttccccgcgc cgagtgtccc
     9541 catctgcggg acgccgcgct ggcgcgcttt tgaactgacc gcatcggggt cggccccggg
     9601 catcgcgcct tctgcctcgc accggacaag gtcggcctct gttccgaacc cgcgctcgac
     9661 cgcccagcgt gcgccctcga ccatgatcgt gttgagatcc ttattcgaga cccgtagcgc
     9721 gctctttgcc ccgacgcctg taggtattgc gccaaaaagc cgctcgatca actcccgttt
     9781 ccttccggcg atatcggcct ctgccagggg cgtcgtgatc agccggacac cacagttgat
     9841 gtcgaacccg accccgccag gggatatgac gccttcagtc atatcgaacg cggcaacgcc
     9901 tcctatggga aacccgtacc cccagtggat gtcgggcatg gcaagcgagt gcttcacgat
     9961 tcccggcatg gtcgcgacgt ttgcaacctg gcgcaccgcg ccttcctcaa gcgtctcgct
    10021 gagcgcttcc gagaggaaga accgtccagg gacgcgcatg cccgggacgt aaccgatcgg
    10081 gacctcccac tcgaggtcgc caaccctatt gattccggca agcatatgga aaaaccccct
    10141 cagacatcaa atataatctc aaccacataa ccatctttat ccctcctgat ctcaagcccg
    10201 gagtaggaca cgcccttgat gagcgtccca cctgcgtgac ggtcaggatc gaacggctcg
    10261 ccgcgtagaa cggcggtgag cctggtgccc tggatgttca catcgaaggt gcagaagacg
    10321 acgttctcga catcggtgat gaagagcaac tccgagaggt agtcaaagag cagggcttcg
    10381 aggttctccg cctcgagttc gatcgtttgc tcgacacccc cgtccttgca gggcccatac
    10441 atctcccgaa agatcgcccg gcccgcctca gcaaagacct cgtccagggt cgcgccccgc
    10501 acccgcagac ggagatcggc ggtgtgctcc agttcctcga aacccatggc ctattaccca
    10561 tcgtaaaaga aggggatcat tcctctgtgg atacaatcga gatagcgcgc ctgatacttc
    10621 gagacataga cggtatagtc acccaccgga atctgcaggt cggttgcttt cggcggttta
    10681 accgacaccg gcagtagaat gggccctcca caggatgtgc tcaccctgaa atcacgtttt
    10741 ctctggagga tgtaggatcg gacatcctca gttatgtaga tgccctgtga ctggcctggc
    10801 tctttaatgt cggtttccat gattgatcac ctatcttggt gctttgtgta aaaagaggta
    10861 tggtttttcc ggttttccat ggttatgttg ttccaaggat ctttgccacc agatccacgt
    10921 actgttcttt cagttcatag acagagttag tcccctcggc gtttcgtctc acgtgcagga
    10981 tacctatccg ggatgcaatt atgccgacca tggatgcgat ggagtggtag gtgatcgcaa
    11041 actgcattcg cagtgtctcg tagatctgtg gtattgtaag tgtcctgacc ttcacgaaga
    11101 ggccgagcac ctcatgccgg agcccatccc ggtcacgtga gagataactt ttgagtcgat
    11161 cctctatctc ttttttgagc tcccccgggg atctcatatg tgcttgtaat tgggtatttt
    11221 atatatatct tttcatttta ttcatttatt ggatattctg acatctggac gacaattgtc
    11281 tgacaattca tgttcatatc cgttataatg acgtcgtccc ccggcggggt ggtgggatta
    11341 atggatgaat ggaaggctgt gagtgattga tagtcccgga taccgtgcca tgggagcagg
    11401 aaggggattt tgagggtgtg tcaacattga cgttagagat cccttccccc ggcactgtat
    11461 cggtggagaa tcacccgggg gtaggggctg acgggggggc gggacgcccc cgcccccatc
    11521 ttagcgtatc accggggtga tgccttttcg caaaaagtat tgtaaaggat ccggccaatc
    11581 cgcatccaga ttaggcaccc ggtctccagt gatgaatcgt cccgggaatc caccgtggga
    11641 ggttcattaa accgggaaaa tactggatcg atgacccagg gctccaacga ccgacaaagc
    11701 aacactcttt atcatttcac aattcttaca ctgatacagc atggaatttg ccagatacga
    11761 cgtcaacaga tactccccgc ggcagatggt ggcgctgccg ctggttatcc tcctcctggc
    11821 gctgatggtg ctcggttaca ccactttaac cacgggcctg cccgtaacgc cgggcatcga
    11881 ctttgcggga ggaacggcgg tcaccctctt cacatccgag agcaaagaga cgatagaggc
    11941 tgtcttcgcc ggctatcctc tgctgtctgt gggtgagggg atcggcgggg ggaggtacat
    12001 ccagttcgga ccgatggatg atgcagagta tcagagcctg gtagcactca taaacgagcg
    12061 ttatccggac gcaaagattg accagatcgg cgagacgttt ggagagacgc tccggagtca
    12121 ggcgttcctc gccctgctct tctcgttcat cggtatgacc atcgtggtca tgatagcgtt
    12181 ccgaaacctt gtgccagcag gtgcggtcgt cctctcggcc ttcgccgaca tagccgtcag
    12241 cgcggcagtc atgcaggcac tggggatccc gctctcgctc gggacgaccg ccgcactcct
    12301 gatgctgatc ggctactctg tcgacagtga tatactcctg accacacggc tactgaagcg
    12361 caaggggaaa ctcgacgaga aacttgccgg cgcgttcagg acggggatca tcatgacaac
    12421 cacgaccctt gcggccatcg ccgcgatgtg ggtcgtcgca acagctggac agatccagat
    12481 cataagtgag atcgcaagcg tcctcctgat aggtctggtg gtcgacctta tgaacacctg
    12541 gatgctgaac gccgggatcc tcaagggata catcctccgg ggggagaaga gatgaatagc
    12601 aaaaccctca agaaacttct caaagactgg cgggtcgctc tgctgctcct gctggtcgtc
    12661 ggctcccttg tcgggatcta ccttatccct ccaaaccctg agaaagggtt tgaagggaac
    12721 ctccagttcg gtcttgacct tgaggggggt tcatggctcc aactggagtt ccagtcggtg
    12781 gtcgtgggct actcgaccac gggatcggtt gatgaccttg tgactaacct tcaaacggcc
    12841 cttgataccg aggtcatacg tatagatgaa aatcaccttg agatccgaaa ggacatccag
    12901 cgtgatgacc ttgaaccgat ctttgccgaa tcaggcgcct cgatcgtaac ctaccagaag
    12961 ggtgtgtccg cctacaccgc ggacgaggtt aagcgaatcc tcaacgagaa ggtgaatgct
    13021 ctcgggatgc aggacgcaag gatccacctg ctcactccga cgggcagcgg tttcccccag
    13081 tatgtgcgga tagaactcgc cggcgttgat atggcaaccg cccaggagat cgtcggcaag
    13141 cagggtgtct ttgagatccg gatccagacc gctggaaacg agacacagca tgttctctac
    13201 ggtgatgtga taacaagtgt cagtgtgccg cagaaggatc catacaccca ggagtggggt
    13261 gtagggttca cgctctccga tgccggagcg gaggcattca gggaggccgc cctcaggtac
    13321 ggcgctgtgg acaacccctc ggctcaccat ctcataatgc tccttgacaa caagactgtc
    13381 tacagtgctc cgctctcaag cgaccttgcg gcacaactcc ggtcggcccc ggtcaggtcg
    13441 ctctcagcca ccacaggcac cggcgatgcg gggcttgatg cggcgatggc gcttgagatc
    13501 cacctgcggg caggcgccct gccggtcaag gtggatatag tcggctcagg gtcggtgcct
    13561 gcggcgctcg gagagcagtt caagacgatg attgtcattg ccgggctgct tgcgctgctg
    13621 actgtagggc tcgttgtata ttaccgatac cgcgaagccg cgatcgtgct cccgatggtt
    13681 gcgatcaatc tctcggagat catcatcctg ctcgggatcg cacggttcgt aatccagctc
    13741 gaccttgcca ccatcgccgg gctgatagcg gtgatgggta ccgggataga ccagctcgtc
    13801 atcatcacgg acgaggtgct ccacgagggc cgggtcccat cgccgagcct ctacatgaag
    13861 cgctacgaac gggcgttcgg gatcatcgcc ctttctgctg caacggtcat catcgccatg
    13921 ctgccgctgg cgctgatgga tctctccacc ctccgggggt ttgccatcat caccatactc
    13981 ggtgtcctgg tcggcatcct tgtcacccga cccgcctatg gaaagatagt tatggcgatc
    14041 ctctcaagat aacaacccta cacaaccttt attgccccgt ttttacacag tgtttgaacg
    14101 ggtatatccg tatgggaaaa ccggttttaa tggatttttt tgccgagtgg tgcggtccgt
    14161 gccaccagca gtcacccatc atcgatgaac tcaggaagag aatgggcgat cgggttgaga
    14221 tcaggaagat cgatgtcggt gttgattctg aggagacgcg gcggtatgct atgaaatacg
    14281 accttcagtt cgtacccacg ctggtcatag agaaggacgg acagatcgtc cggaaactgg
    14341 tgggtgtcca ggacctggcc acgcttgaga gcatcctgaa accgctggtg gagaagtgaa
    14401 gatcgggatc gtcggcggaa ccggtgagat cggggagggt atggcgctcc gcctctcgcc
    14461 gaggtatgac gtgattgtag ggtcgcggga ggaggctaaa gcggtcatga cctgcgaggc
    14521 ctgtcgggag acacttgagg ggcagggcat ctcctgcagc ctggtcggcg cctccaacca
    14581 ggctgcggtt gacggggcgg atctcgttgt cctggcgata cccttcaaac acgtggcgcc
    14641 aaccctgaag acgctggggg gatttgaggg caagatcgtt atcagccccg tcaacccgat
    14701 cgagcgggag acctactttt actacgcccc gccgcccgag ggctcggcgg cgatgatgat
    14761 aagaggtatg cttcccgcga ccgcgaccgt atgcgcagcg ttcaacaacg tcgctgcaaa
    14821 caggtggaag aagatcgacg aggaactgga ctactcggtt gctgtctgct gcgacgatcc
    14881 ggcagccaaa cagacggtga tggagatggt caacaacgtc tcacgcctcc gcgcctacga
    14941 tgcgggaccg cttgcggccg catcgatggt cgagagcgta acacccctcc tcctaaacat
    15001 cgcccggttc aacaggatgc gggatgtcgg cgtcaggttc atctaatacc tcttttcgta
    15061 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccccgcc ggaggcctcg
    15181 agcgagtacc gcgtccaccg ggcggtatat cggacgacac ctcaccgtgc aatcgtccac
    15241 gcccacccgg ttcacgctgt cgctgcatca ctcgatctcg acttaatacg gccagtcgac
    15301 agtgagggga gaatgctctg cccggagatc ccggttgtta ccggagagcc cggaaccgat
    15361 gagatcgcaa gaaacgtcgc cgaagcgctc atcagcgggc gtatcgccat agccagaggg
    15421 cacgggacgt ttgccgccgg agagaccctg gatgaggcgt atatctacac ctcaatcgcc
    15481 gagtatgcct gccggatcct catcctatcg gggaggcttc ggagggttgc gtaactgctc
    15541 gatctggcgg atcgtctcgc gttgaaatcc gagcagcata tcgcttatga ccccgaacat
    15601 gaacatctgg aaccccatca tgatcagcag gacggtgagg attgtaagcg gcaggtgctc
    15661 gatgttcttg aaccactcga gaaccacgta gatgccgatg accccgccta tgatcgagat
    15721 gatgagcccg atgatcccga agtaaaagat cgggttgttc atcttcgcaa gacggtatat
    15781 ggttgagaag atccttagac cgtcctggag tggatttaac ttggtgaccg tccccggtct
    15841 tgcagagtag cggacgggaa cgaccgctac ccgctgcccg gttctcaccg cttcgaccgc
    15901 catctccgtc tcgatctcaa atccggcctc tttaaggttc atctgccgga tcgagtgaag
    15961 ggtaaaagcg cgatagcccg agaggatgtc gtggagatcc ttaccgtggg cgaccctgaa
    16021 gacgtggtta aggatctggt tgccaagaag gtttaacctc gtaaaagccc ctttctccgg
    16081 gcaggccagg cggtctccga tgacgtggtc aaaccctttt ccgaggggct caagcatcct
    16141 ctccgcatca tccggggagt atgtcccgtc gccgtcgagc atcagcacgc ggggtttgtc
    16201 tatgatctcc agagcctcta agatggcgtt gcccttccct ttccctgtct gcgtccgcac
    16261 taccgctccc gcggcgcgtg ctatctcggg cgtcccgtcg gtgctgtttc catcgatgac
    16321 aaggatgtgc tcatagccgc gctccttaaa ccccctgacc aggtcgccga tcgtcggggc
    16381 ttcgttcagc gtcgggatga ggacacagac ctcgtcgcgc tcgatgctca tcgaatgtac
    16441 tatatcccgg tcaggaacaa taagcacttc gtatgcacat agccaaaccg gggctgcgcg
    16501 ctctaggcgt cgcggagagt tactccggcc gcgaatactc cacgcttgcc ggtgtggtca
    16561 tgcgtaagga tctccggatc gacggcgtgg gttttgccag ggtgaccgtc ggtggcatgg
    16621 atgcaaccga tgcggtcatc cggctctaca gccgtcttgg acgccgggat atcaacttcc
    16681 tgatgatcgg cggaaacgtc atcgcctggt ataacatcat cgaccctgca gcagtgcacg
    16741 aggcgacagg tctcccggta atcatcgtca catacgagga gtcggagggt ctggaggagg
    16801 atatccgccg ccacttcccg ggcgacgatg caagactcgc cgcataccgg aggctcggtg
    16861 agaggatgcc ggtcaggctg cggtccggct gcaccctgtt catccgctcc tgcggcatcg
    16921 caggggaaga tgctgcgcgg ctctgcaacg gtttcaccta cgaaggaagg gtgcccgaac
    16981 ctatccgtgt ggctcgactc atcgcgagag gggttgtccg ctcctcagga agtccggggc
    17041 tcgcccctga caaccatgac cgttgaggtt gcgtgatcga caacgtatga actggtgctc
    17101 cccaggaata ggcggccgag cctgcccttg ccgtgcgacc cgacgactat cagaccagcg
    17161 ccgatcttac gcgcaagcgc gatgatctcc gcaccggggt gccctaactg tttgtggatg
    17221 gttaccggga caccggcggc cgccgcggtc ctctccgcgt cagccaggat ctcgtcggcc
    17281 tcccggtcga gggcatcaag gagggcctgc tgtgcgacat cgggtggagg tagatcgctc
    17341 agggcaagcg cggggtatgc agcggtctgg atgacgtgca cgacgtgcac cgggatctcc
    17401 atcgcgctgg caacggcaag cccctgttca agcgccctct ggccgatatc cgagccatct
    17461 accgccacaa gtatcgatct gaacataaca ctctcttagg agtgtccctg tagaaaaagc
    17521 atgaccctcg ggtcttcagg cccgcactgc atcttttgag atcaccttcc cgtcgcgttt
    17581 atcgatgatc tcgacgcaga ccttggcacc tgggtagagg gtcgtatcgg tgaggtctat
    17641 atctatcctc tcgccagggt tccagtattc ggtgcggcag cccgcaccgc ctatggtctt
    17701 caccccgtaa tgatgcgacg ggatgaagag gtagccgttc agggtctgga ctatacagac
    17761 cttctggccg ttcacgtaga agaccgcctt aaggcaatcg ttctcgtata ccgtgctgcc
    17821 gttgttgagg agggtcaccc tgctcgcgta ggtcattttg ccggtaatcg cgctgtggtg
    17881 cgaaacccct gttatgatga tgggtggctg cgccggttcc gcccacgacc aggacgggat
    17941 catcgcaagc agcatcagca ggacaagaac ggcaagtatg acggtgacac cgaccaggat
    18001 gagaacgccg tgggtctcag aaacaccgct ctcgctggct gcagtccggc gagaggggcc
    18061 cgccgacctc ctgggcgatc gggttctatt aatccattcc gtagatccgg gcaaggctct
    18121 ggcagagtct gcaggtgtat cgatccaggt ctctgcagat atcaagtctc ctgttccacc
    18181 cgatgacagc ggcatgctcc cactggatcc cctcaaaaac ttcttcctcc gatatgacaa
    18241 agaccttcgg atatgatctc agtcttatcc gctcctcaca gaggcgactc ctggcagata
    18301 cagcggccgg gtgacacgcg ggagagagcg gcgggtggag atcctgtttg gcgccgggag
    18361 gtgggtggtc agccgcaaaa gacgtctcac cgcagtggtg gttccgaggc catcaaaccg
    18421 tgagcgaact ggttttaggg gctcagactc gccctggaca aacttacttt tcctcaaagc
    18481 aacaagatga gtatcatggc agaaccagag cggctgcccg gatacctgcg cctccaccgc
    18541 agcggcgaac tggaggagcg ggcagagagg gcgcatgagg tgctccgcga ctgcgttctc
    18601 tgccctcgtg agtgccatgt gaaccgcata gaggacgaga ccgggttctg ccggacgggt
    18661 ctcctgccgg tggttgcaag ttacaacccg cattttggcg aagaaccgcc gcttgtcggc
    18721 cggagggggt ctggaacaat cttcttttcg ggctgcagca tgcggtgtga ttactgccag
    18781 aacttcgata tcagccaggg agggatcggt tacgccatct cctgcgacga cctcgccggg
    18841 gtcatgctcc ggttgcagga gatggggtgt cacaacatca acctggtcac gcccacacat
    18901 gtcgttccac agatcctccg ggcggtcacc atggccgctg gccgtggtct gcgcataccg
    18961 ctggtctaca acagcggcgg atacgactct gcggagacgc tgcgactcct tgacggtgtc
    19021 atcgatatct acatgccgga tgcaaaatac gggcgggacg atgcggcgct ggcgctctcg
    19081 cacgcgccga actacaccgc cagcatgaaa gctgcattaa aggagatgca ccggcaggtc
    19141 ggcgacctgg tgatcgagga cggtatcgct ctcaggggca tgataatccg gcaccttgtg
    19201 ctcccggcga accttgcaaa cagcgagatt gttatggagt tcatcgcccg ggagttgtca
    19261 cgtgactcat acgtgaacgt catggcccag taccgcccgg cagggagagt gctgcagggc
    19321 aggctaaacc ctgagtttgc agtgctgcgg cgtccgatca ccgcggagga gtatgcctac
    19381 gctctccgat gtgcgcggga gaacggtctt tatcgcggga tcttctgaga ggggccgcaa
    19441 aaagactatc tggttgaacg ggaagattat actcttgatt agaggtgaaa gacaagggct
    19501 ggctccggta tgcggcgatg accgatatcg gtatgcgtga gcgaaacgag gatgcctttt
    19561 tcgccggcag ggtgagcggc taccatctct ttgccatcgc agacggcctc ggggggcacg
    19621 cctgcgggga tgtcgcaggc aggatggcgg tcgagatcct ggaggaggcc gccagcgaag
    19681 aactctctgc gtttgatcct gcggaggcgc tcaaacgtgc gttccggcgg ataaacaccg
    19741 cgatctttgc cttcaaccgt gaacgtggcc tgaacgccgg aacgacgctt tcggtggcga
    19801 ttgtgggaga tgcgggcagg tgctggatcg gcactgtcgg cgacagcagg acgtatatca
    19861 taacaccatc ctcggtctgg cacacgcgcg accataccta cgtccagagc ctggtggacg
    19921 cagggatgat atctccggca gaggcgttct atcacccggc aaagaatatc ctgaccaggg
    19981 cgcttggtct ggaagcccgt gtggagatgg atctcgacga gagggaactc tctccagact
    20041 cagtcctgct gctcagttct gacgggcttc acgactacgt cccgggggat ctcatccagc
    20101 agatcgtgat ggcaaacgat ccggatacgg cgtgccggaa gctgatcgat gccgccaggg
    20161 aagccgcaag caccgataac acaacggtca tcgtcgtgaa acccctgctt ggtgcccggc
    20221 atccctgagc gcctcaacga tggccgcgcg gacagcctcc acgctctcgg tatcgagggc
    20281 ctggcgcagc gccctgatcg cctcaccgcc gccgatctga ccgagggagc gggcagcgct
    20341 ccgccgcaca aactcatttt cgtcgtgctg aagggctgca aggctggcga ccgcccggga
    20401 gtcgccgatc agcccgagcc cttttgccgc catgtagcgg acgtggtcct tgctgtcctg
    20461 cagggcggag agaagagggt catacgcttc atgcccgccg atcaacccga gcgtctcggc
    20521 cgcacggtaa cgcaccctcc agtcagtgct ctccagcagc gagatgcagg cgggcacggc
    20581 cggtcttccg atagccacaa acccgcgcat cgcctcctcc cgcaccccct tctccgggtc
    20641 tgagagcatc gagatgagtt cctttatccg gctgtccaga cgatcgcctc cgggtgagat
    20701 attcttcggg gtcatggtgg tctcacatcc gcggtgtatg atggggacat cgatcatacg
    20761 ttgcagcgac cgcataataa gtcctcaggc ccccggctag acgctggagg cgggccgtta
    20821 taccgggggg cgccttatgc tcatcagatc agatttccga tgagatagag tataaggcag
    20881 actgaaagaa aaattcccgt tccgggtatg actctcccga gagcattgaa tatgggatcg
    20941 cttgcaaacc tgttctcctc agcgtacatg gaaacatgtt ctttatcaga ggatatatat
    21001 ttttttatta gaataaaata taaaccgact atttaacgtt ttccaattat ttctcccgtg
    21061 gaaatgccgt atttcacgaa ttacagaaag ttacggggtt tggcaataca aacagcggaa
    21121 gtgtgaactg atccttatta aacagagtgg agagaacgtt ccgccagacc ggagaaaaaa
    21181 atgacgccca ggacggaatt cgaatccgtg tcgacggcgt gacaggccgt catgataggc
    21241 cactacacta cctgggcaca tcgttgtgag gatcccgcct cccggattcg aaccggggac
    21301 atcgcggtag ccgcgcagtt cgcccgaaag cgatgaccat actacagccg cgcactctac
    21361 cagtctgagt taaggcgggt ccctgctcta acatggttag agcgatttga tattaaacat
    21421 ttcgcagaat cggggactgt tgcggaggag gccatcaaac caacagccgc actggtttga
    21481 taaacctctc cgtgtccagt ccatggacgg tccatggaca gat
//
LOCUS       DAOSZX010000018        20727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2107_length_20727_cov_19.284298, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000018 DAOSZX010000000
VERSION     DAOSZX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 20727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20727
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_20727_cov_19.284298"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..361)
                     /locus_tag="PK196_02840"
                     /pseudo
     CDS             complement(210..361)
                     /locus_tag="PK196_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062264490.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            405..596
                     /locus_tag="PK196_02845"
     CDS             405..596
                     /locus_tag="PK196_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40393.1"
                     /translation="MTQSLRTGKHQTEWITISVDEYESMCRTIEVLSDPDLMQQIEEG
                     KKKGTKVQDFEEVAREIGI"
     gene            complement(593..898)
                     /locus_tag="PK196_02850"
     CDS             complement(593..898)
                     /locus_tag="PK196_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HON40394.1"
                     /translation="MKWNHSSVAEEEQKIRMATFRIQIREPAKKWLKKYDIEIQRRFA
                     QKIRKLSEDPEMHGKPLRGSLHGYWELYFERSFRIIYTVDNKEMVVTIEAIKHKDGF"
     gene            1133..1447
                     /locus_tag="PK196_02855"
     CDS             1133..1447
                     /locus_tag="PK196_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40395.1"
                     /translation="MDIQVMSSWNVYTQAIKNNPKVTAEGRGKRDHEDMVAIVFRDSG
                     EQVLQMEMKLNYFYIDTMDGKRYRVMSKNEVLGFSGEPGVSYTIDLPKLIETEIKPRL
                     KA"
     gene            1548..1994
                     /locus_tag="PK196_02860"
     CDS             1548..1994
                     /locus_tag="PK196_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40396.1"
                     /translation="MEKGSLEKMGAAFTEMNRHFEEMYQATFAIPEEALRERKNGSMQ
                     VATFHFNWVFGEADGHEYLEFYRFHRFGDEHARIWEDGTIEQLDILETMYGYNPKIPG
                     DEERKSEESARRYENLLKELAKAGLLEDMPYHKIMNSFLVLRKEEK"
     gene            complement(2338..2793)
                     /locus_tag="PK196_02865"
     CDS             complement(2338..2793)
                     /locus_tag="PK196_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HON40397.1"
                     /translation="MTTLEDCFRERMLRRVTPSAEKAKESLSLAQSYLDEARQIAGIG
                     ARRMSLTGAYMAWFHAARAVLFRDGIREKSHYCIELYLNHYAQSGDLEEEWVLMFGRL
                     RTQRHESQYSFGPAPTDAEVLAAIEHAGQFIERIRRLLSETRRREWDGR"
     gene            complement(2780..3337)
                     /locus_tag="PK196_02870"
     CDS             complement(2780..3337)
                     /locus_tag="PK196_02870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HON40398.1"
                     /translation="MGKPALTELFKTEERIRILRHVAGQRQVTTTAVAGATGTSKPLV
                     SRYLRLLVKGGFCTQRGRAYTWNENARSLAVKRLLNIDLLAASVPLPEWARGIGIYGS
                     YAEGTNTADSDLDLWVLVDEYTPDLEILAARVEKDASAAAGTEANLLILTPERLSDLR
                     ETDQPFYTNLIRSSVTLGGASIDNA"
     gene            3584..3751
                     /locus_tag="PK196_02875"
     CDS             3584..3751
                     /locus_tag="PK196_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40399.1"
                     /translation="MAVKVRREKKSEFVAKLEEVLPLLRERFGVARIGIFGSAARGEE
                     RRGRAGRVCAR"
     gene            complement(4163..4630)
                     /locus_tag="PK196_02880"
     CDS             complement(4163..4630)
                     /locus_tag="PK196_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40400.1"
                     /translation="MGYPLFALNAERFETNLVEAAGGICVNKRLKRKGKPGVNITVDE
                     LNELNPEYMFISGFLSSPASDYLHYCTEHGIVGDAVGAGRVYNVPAGWDFGSPRWILG
                     FMFLATTLHPEIFSFDLEEEQREFYRRFYGITGDKFVANRDFSRPVVGAPEDE"
     gene            complement(5037..6848)
                     /locus_tag="PK196_02885"
     CDS             complement(5037..6848)
                     /locus_tag="PK196_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HON40401.1"
                     /translation="MVTGITTFQSTKIFLNDILKGINERKIQLPDFQRGWVWDDYRIQ
                     SLLSSVSLSFPIGTVMLLQTGNTEYRFKPRPIEGLPSEPVVAPEQLILDGQQRLTALF
                     QALIKPGPVFTEDARKKPIHRYYYFDIERCLDPNEDREDCIRSIPEDKVIRDFRGMAI
                     EDYSTPEKEYEAGLFPVKKIYDRNSSWRMGYEEYWDYDKSKIKLFNQFYAEVIQRFEH
                     YQLPVIEMAKETPKEAVCLVFEKVNTGGVALTVFELLTATFAADNFPLREDWKDIEEK
                     FGGEEVLKKIPNDYFLQAVTLLTTMKRNQEYMDRGDPDKRAHAVNCKRKDILRLNLDD
                     YLEQRDAAVDGFMKAAKFLRIQKIFTARDLPYQTQIVPLAATFAILGGKDDNDTVRQL
                     LSRWYWCGVFGELYGSAVESRFAKDLPELVAWIHGGDEPTTVKEALFSGRRLEELRIR
                     LSAAYKGIFALLMRDGCEDFRSGQPIDITNYYDENVDIHHIFPRKWCSDNLGGLELSK
                     HRMAVESIINKTPLSARTNRMIGESAPSVYLRRIEKNEGITSERLDQILRTHVIDPEA
                     LRNDDFWTFYNLRYEAILDRIEAAMGKTVIRRDAEHV"
     gene            complement(6860..7006)
                     /locus_tag="PK196_02890"
     CDS             complement(6860..7006)
                     /locus_tag="PK196_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40402.1"
                     /translation="MGDIRIFCIDGAEPRELKGSSVALEKLLQNSIEYEIGAVRTSYN
                     LIKH"
     gene            complement(7012..7440)
                     /locus_tag="PK196_02895"
     CDS             complement(7012..7440)
                     /locus_tag="PK196_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF365 domain-containing protein"
                     /protein_id="HON40403.1"
                     /translation="MTSITGVTFPVPKHLMPRFFSAGKTVFIKPATVYRELRSGMKLV
                     FYQSREDTGYVGEAAIRRIILNDDPFAFFETFGDAVFLTPEETRAYVESQERWQGVRV
                     RKGEPRKRPWMALELEGITKYDTAKKPERFVPVGGRYLRG"
     gene            complement(7455..7898)
                     /locus_tag="PK196_02900"
     CDS             complement(7455..7898)
                     /locus_tag="PK196_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HON40404.1"
                     /translation="MEHWLAISNRDNSKVVIKKHVWGVPKRHINTIARTEPGDTLLIY
                     VGQQVIDKETTLPPAITGCFEITSTVYEDSSRIFTAPPNLGDEIFPLRVKLQPVMIFD
                     PPIEFKPLIPNLAFITNKKQWSGHIRGQAMRTIPEEDYRLIMSQG"
     gene            complement(8401..8742)
                     /locus_tag="PK196_02905"
     CDS             complement(8401..8742)
                     /locus_tag="PK196_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40405.1"
                     /translation="MAITAIMNIIGGLVLCAGIIGALPVLGGFLEKAGKFLGGFQVII
                     GIIMIILGLLDIFSLQGIVAFLVGVILLTGVFHVVPALGVYLEKFGKWLGGFQTIIGV
                     IAIIVGILGLL"
     gene            8837..9247
                     /locus_tag="PK196_02910"
     CDS             8837..9247
                     /locus_tag="PK196_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40406.1"
                     /translation="MDVALLRTLLTAGVSLIPDHSPSAEDRFEELLSTCESALERNVL
                     EAIYKAGLPLPDGGQEVIAEGDEMIARPDFIYRRGGHSIAIFVDGPDHERETIKRDDM
                     QKRGRLDLMGYTVLSIGYRDSLEECIRSLTELLE"
     gene            complement(9401..9718)
                     /gene="eif1A"
                     /locus_tag="PK196_02915"
     CDS             complement(9401..9718)
                     /gene="eif1A"
                     /locus_tag="PK196_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HON40407.1"
                     /translation="MTTTPRGSGKNESEEIIRVRLPKKKNQEIFGRAELMLGANHIRV
                     RCEDGVTRTGRIKGKIKKRLWIREGDLLIVVPWSFQDEKCDIVYRYIRPQVDWLQKHN
                     YIK"
     gene            9944..10204
                     /locus_tag="PK196_02920"
     CDS             9944..10204
                     /locus_tag="PK196_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HON40408.1"
                     /translation="MESNKLYVGNLTYSVNKEQLKELFGQYGTVNDVRVIERKGFGFV
                     EMSSPKEAEEAKEALNNTVFEGRTLKIDEARPQRPRSDFRRY"
     gene            10450..10692
                     /locus_tag="PK196_02925"
     CDS             10450..10692
                     /locus_tag="PK196_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40409.1"
                     /translation="MEIHVDKDACVGCGLCVKDCPMHVYELQNNVSVAVRPNNCMGCL
                     SCHEICPAQALEHRGIYAAKRHYIDIHVCEMLRKVI"
     gene            10692..11255
                     /locus_tag="PK196_02930"
     CDS             10692..11255
                     /locus_tag="PK196_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HON40410.1"
                     /translation="MTAGYVDEMHEAFQTDLVYRSEDVPLECSPKPKDLEQTLHGLMK
                     LNGLVIRSLEEIAGRGANAVTYRAGKKFGHEVAQYFQKRDDVEGALHELSDLLRGQYS
                     FEVWKPEDKDSYIIEENGETFIYLVFHDCIVRQTLRRNGLDQGGPLCQTLFGYVVGAI
                     EEITGRKAKLEIVHTGPNACLKKLVLK"
     gene            11266..12183
                     /locus_tag="PK196_02935"
     CDS             11266..12183
                     /locus_tag="PK196_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HON40411.1"
                     /translation="MKIAIEELAGCSGCTIAVLDLHEMLLDVVDKADIVYSPVIMDVK
                     EPPEGIDIAFVTGAVRNAENQERLEKIRRRAKTLVALGTCACYGGVSGLSMLHSNDEI
                     FSYVYRGVETVKPDGVIPTDVPPFLYRAFAVGDLTKIDYYITGCPPKEIYLKQIIPAL
                     VSGDKLNLSRKSVCADCDRKMGTVENWTLKRRYEGVPDREHCLLGQGYLCLGSVTFGR
                     CGAACPKNNVPCHGCNGPSLDILREPCRDIYGMMVRRISDLTDKPQKEVEKELYDIAH
                     TMYPFTIGSLIMEDKEVSKIRDLVKERSR"
     gene            12180..13547
                     /locus_tag="PK196_02940"
     CDS             12180..13547
                     /locus_tag="PK196_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HON40412.1"
                     /translation="MKEVTINPVTRIEGHADVRIYLNDRGEVDSAHFQVVELRGFEKF
                     LSGAAIEEAPRITPRICGICPTSHHLAAAKATDQIFGVEPPATAKKLRELLNLGQYIH
                     SHSLHFFMLAAPDFILGHDCPPETRNVIGLARHSPDLAKKAITVRKFGQRLTEAIGGK
                     PIHPSNALPGGMSAPLTAAKRAELVAMADEALRIALEGWDIARTLLDGVDTEFGAVRT
                     GFMGMTNHGVYSTYDGPVAVLNREGGRIGSFTGEDYTKFIEEYSVDWSYLKFVRLKGG
                     ENYRVGPLARLNIVEKMGTEHADAALAEYRERFGTVSQAALAYNLARYVEFIASCERA
                     VKLLSDSGITGTDVRTPAGEVVNRRGVGIIEAPRGTLIHDYTVNDAGIIERCNLIVAT
                     CQNNYAIDRGVEDVARRVVKNGALSNGAANQIEMVIRAYDPCISCATHAIGRMPMKIE
                     LVRKA"
     gene            13561..13917
                     /locus_tag="PK196_02945"
     CDS             13561..13917
                     /locus_tag="PK196_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HON40413.1"
                     /translation="MESMLKQILGEFLKLDGVTAAVVAGRDGFVIESVVAGDVDVEAL
                     GAMASTGMGTSEAMSNELGKGEMHQMLVELENGPILLSPLSEDELIAIVANANVNVGR
                     IRYELKKNKDRIIAAL"
     gene            13922..14584
                     /locus_tag="PK196_02950"
     CDS             13922..14584
                     /locus_tag="PK196_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HON40414.1"
                     /translation="MLPDGISLGRLQGQLSTLASISPHFTGAVRITEKGGGTGFVLFE
                     AGNPYAAAFEDKSRGLLLGGDDAYRHFLERPSLVYELIAYTAEETRAAREASSASGCL
                     VTGVGRAPPSPEKAALQQIVRQPGVVAVSVFHEGFALQSVGSADFEKVAAVAEDLLRA
                     GSQITAEMEMGGLSQVILETPRGKFIIAPYNDLYLCILAEPDANLGLIRLSIRGMQEN
                     RG"
     gene            14701..15647
                     /locus_tag="PK196_02955"
                     /pseudo
     CDS             14701..15647
                     /locus_tag="PK196_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843848.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
     gene            complement(15681..17153)
                     /locus_tag="PK196_02960"
     CDS             complement(15681..17153)
                     /locus_tag="PK196_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON40415.1"
                     /translation="MFVKKTKSLCPTCGRVLDADVVEEEGQIWLVRTCPEHGLYRGLY
                     WSDADMYRRFDAYERIGNGVSNPQKTPSATGCPNDCGLCSNHRSTTLLANIDLTNRCN
                     LDCDFCFANARACGFVYEPTFEQVVGMLRMLREEKPVPAPAVQFSGGEPTLRDDLPEI
                     IRVAKEFGISQVQVATNGIRLARDVDYVQELKNAGLSTVYLHFDGLTRETNSKLGSDL
                     RAIENCEKIGMGVVLVPTVIKGRNDHEAGAIIKYAAEHFETIRGVNFQPVAFTGAASA
                     DDVRKERITIPELAERIEEQTDGIIKKDHFYPVPCVVPISDLVEAYTGKPQIRFTTHQ
                     HCGAATYVFVTDEGMIPINRMVDVDAFFESVEKMAARLAKGGSLNKYVTLVEGVKDIY
                     TSTRKAEYKNTGELMKLIMKALVLQNFEALRDFHWNALFIGMMHFMDRYNYDLSRVER
                     CCIHYATPDGRLIPFCTYNSGPVHREQVWKKFAQQPREDE"
     gene            17295..17795
                     /locus_tag="PK196_02965"
     CDS             17295..17795
                     /locus_tag="PK196_02965"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HON40416.1"
                     /translation="MLPAYLPNSAAAVFGGGTPIDLGRTFSDGRRVFGDGKTYRGFFG
                     GVLSGVLVGLIQILAASAFGLDMLPRHTILSVILLATGALLGDLVKSFFKRRLGKERG
                     EPWIIADQYDLVIGSLLLVLLVYPEWLVENITLPILVWIIVLTPLLHRVVNIIGYYIG
                     VKEVPW"
     gene            17789..18301
                     /gene="pyrE"
                     /locus_tag="PK196_02970"
     CDS             17789..18301
                     /gene="pyrE"
                     /locus_tag="PK196_02970"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HON40417.1"
                     /translation="MVNRIARLLLESGAIEYGDFLLASGARSSYYIDIKAATTNPAIL
                     AEIGKAIAEGREFEVVAGVAVGGVPIAVAVSLASGRPYAIVRKEAKDHGKSGSIIGEV
                     NGKDVLLVEDVTTSGGSAIYGIEALRAAGAHVDRVVTVVDREAGAREALAEKGVSLQA
                     LVRVSDLLDG"
     gene            18328..19620
                     /gene="purD"
                     /locus_tag="PK196_02975"
     CDS             18328..19620
                     /gene="purD"
                     /locus_tag="PK196_02975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HON40418.1"
                     /translation="MDMKLLVVGGGGREHAITRALSCNSDVSIFSVMAQKNPGIARLA
                     ERVLLEKETNIQRILPFATTCGVDAAIIGPEAPLEAGIVDYLESAGIPCVGPTRAAAR
                     IETDKAFCRRLMERYGIEGCPYYRVCHDPEEARRFIESYGGDLAIKPIGLTGGKGVRI
                     MGEHLDAQGAIDYVRHLGGNVVLEERLIGEEFTLQAFVDGEHLVPMPLVQDHKRAYEG
                     DVGPNTGGMGSYSLPDHMLPFVSRPDYEKALRIMQDTVAAMRAEGTPYRGILYGQFMN
                     TADGPKVIEFNARFGDPEAMNVLSIIESDFTEIVQRIIDGDLSSSDVSFANKATVCKY
                     VVPEGYPEAPKVGEPIVLGEYGDALLYCANVEERGGRLYTRTSRALAFVGVGETLEEA
                     ESIAERAASSVSGRVFHRSDIGTRESLEKRCRHMREIA"
     gene            19617..20546
                     /gene="argF"
                     /locus_tag="PK196_02980"
     CDS             19617..20546
                     /gene="argF"
                     /locus_tag="PK196_02980"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HON40419.1"
                     /translation="MKKDFLSILDMESDELEGIIADAIRLKHLKSDGVAHEYLRGKNL
                     GMIFEKASTRTRVSFEAGMSDLGGHALFLNPQDMQLGRGEEIRDTARVLSRYLDAVMI
                     RAYSHATIEEFARYSTIPVINGLSDRLHPCQVLADIMTLRERFGDLRGLKLAWVGDGN
                     NVCNTWILSTPLTGMEVVVASPPRYRPEDGVVERARAAGGRVTILTEPEEAVRDADVL
                     YTDIWVSMGDEHESTERLQAFQGYTIDSRLLELAVPGAPVMHCLPAHRGEEITDDVLE
                     GPQSIVWDQAENRLHAQKALLVWLIRGGMPPEG"
BASE COUNT         4412 a         5881 c         6022 g         4412 t
ORIGIN      
        1 aaggcgttcg gtgcggaggt gggggtgcat ggggggcatg tgcgggggtg ggtggggttg
       61 atgaggtgtt tggccggttc gagattatac tcttcggcag cattgtgcca gcggttgttt
      121 ggaagagccg ttttggatcg tatggtgagt ttggtggttt ttcaacttac ggagaatttt
      181 gcataatttt aagctggctt ctttgtatga aggaagacat ggatgcaagt catccacgcg
      241 atgaacggtt tgggatattg gaacggccaa ccgcgcttgc cagcattcat ccgggcaagc
      301 agatcgtccc actgctcgat gaagtcaagc gcgagataga attctccgcg ccgcaccaca
      361 cacaaacgtt aatatacagt ctgggcaact ctgtagatac ggatatgacc caatcactcc
      421 gaacggggaa gcaccagact gaatggatta ccatctctgt tgatgagtac gaatccatgt
      481 gccgaactat cgaagtcctc tccgatccgg acctgatgca gcagatcgaa gaagggaaga
      541 agaagggcac gaaagttcag gattttgagg aagtggcaag agaaatcggg atttagaatc
      601 catccttgtg tttgatcgcc tctattgtta caaccatctc tttgttatca acggtgtaga
      661 tgatacggaa actccgttca aagtaaagct cccagtatcc gtggagcgag ccccggaggg
      721 gcttcccatg catctcggga tcttcagata atttgcgaat cttctgggca aaccttctct
      781 ggatttcgat atcgtatttc ttgagccatt ttttcgcggg ttcccggatc tgtatcctga
      841 acgttgccat acgaatcttt tgttcttctt ctgcaacact actgtgattc catttcataa
      901 taaaacctga cgattggtga ttattttaag aaaaataggt gcccggcgtc catacggtgt
      961 ttgataaacc atgtgtaggc tcaaaacgca tgcgccatct ggcatataaa aaatggtcgt
     1021 aacaggtcag gacacccctt catcacacct tgctcttacc catcatatca caatccccct
     1081 tttctgaaaa tacgcccata caaccgttag atctttatat aatgagagat aaatggatat
     1141 tcaggttatg tcatcatgga acgtgtacac acaggcaata aagaataatc ctaaagtcac
     1201 tgccgaagga agggggaaac gggatcacga agatatggtc gccattgttt tccgtgacag
     1261 tggtgagcaa gtattacaga tggaaatgaa actcaactac ttctatatcg acacgatgga
     1321 cggaaagcga tatcgtgtaa tgtcaaagaa cgaagttctt gggttttctg gtgaacctgg
     1381 ggtgtcatac actatcgact taccaaaatt gatcgaaacc gagattaaac caagattaaa
     1441 agcatgagtg actcatcagg cattggtaca acagagcctc tctggatgcg gttctttccc
     1501 ctcaggccgg agttaggttt atatggcgac cctgagtctt atcgggtatg gagaaaggct
     1561 cgcttgagaa gatgggtgcg gcgtttacag agatgaaccg tcatttcgag gagatgtatc
     1621 aggcgacatt cgcaatacct gaggaagccc tgcgggagag gaagaacggg tcgatgcagg
     1681 ttgcaacctt tcactttaat tgggtgttcg gggaggccga cggtcacgaa tacctggagt
     1741 tctaccgctt tcatcgtttt ggagatgaac atgcccggat atgggaagac ggaaccatcg
     1801 aacaactcga tatccttgag accatgtatg ggtataaccc gaagatcccc ggcgatgagg
     1861 aacgaaagag cgaggagtca gcgcgaagat atgagaacct gctcaaggaa ctcgccaagg
     1921 ccggtctcct ggaggatatg ccctatcaca aaataatgaa ctcgttcctg gttttacgca
     1981 aggaggagaa gtaacaattc agtcttgcat cgattccgaa ttatcggacc ctcatgaacc
     2041 cctgtttacc gggcagtgga catttggggg ggaccacgga gaggctcgcc ctccgcaggc
     2101 ggcacgactc acggaccccc catccggctc gccgccggtt ccacccaaaa aggatccgcg
     2161 gcatcccgtg caaccgccgg gcaaaggagc gtgtccccgg gtcactcgcc ggcatgctgt
     2221 cttgaggatc tcttcgggcc ggagtggttc ctgtcaccgt cggcggtctc ccctcattct
     2281 gaccagtacg tggagcggga ggtagtctgg tgtgaagcgt gatcctgttc tcctcgttca
     2341 tctcccatcc cattcccttc tgcgcgtctc agagagcaag cgccgtattc tctcgatgaa
     2401 ctgaccggca tgctcgatgg cggctaaaac ctcagcatcg gtcggcgccg ggccgaacga
     2461 atactggctc tcatggcgct gtgttctcag cctgccaaac atcagcaccc actcctcttc
     2521 gagatcccct gactgcgcat agtggttgag gtacaattcg atgcaataat ggctcttctc
     2581 gcggataccg tcacggaaga gcactgcacg ggcagcgtgg aaccatgcca tatatgcccc
     2641 tgtcaggctc atccgcctcg caccgatacc ggcaatctgc cgtgcctcgt caagatacga
     2701 ttgggcgagg ctcaacgatt ctttggcttt ctccgcggaa ggtgtgaccc tgcggagcat
     2761 cctctccctg aagcaatcct caagcgttgt caatggaggc acccccaagg gttacgcttg
     2821 accggatgag attggtgtag aacggttgat cggtctctct caggtcagac agcctctccg
     2881 gggtgaggat gagcaggttt gcctcggtcc ctgctgccgc actcgcgtct ttctcgactc
     2941 tcgctgcaag gatctcgaga tcgggtgtat actcatcaac gagcacccag aggtcaaggt
     3001 cgctgtcggc cgtgttcgtc ccctccgcat agctcccgta gatacctatc cccctggccc
     3061 actccgggag cggaaccgat gccgcaagga ggtcgatgtt aagcaggcgt ttcactgcca
     3121 gacttcgggc gttctcattc caggtgtatg cccgaccacg ctgcgtacaa aagccgcctt
     3181 tgacaagcag gcgcaggtag cgtgagacaa gaggttttga ggtcccggtc gccccggcaa
     3241 ctgcagttgt tgttacctga cgttgcccgg caacatgccg gaggatgcgt atcctttcct
     3301 cggtcttgaa gagttcggtt aacgccggtt ttcccatgtt gtttccgcct cgtgaagaga
     3361 atgtttccgt tctggaaaca aaatgtttcc gatccagaaa catgggggca cggggacaga
     3421 gccccccgcg gcacccgcag ccacccgggc ggttctggtt gatcagggag atgattgcag
     3481 aagaagcata gaatctggaa ttcgttcacc ctgtaggcca gagccgcgga cgataacgtt
     3541 catcatcttc tcatccccag gtacgatcag gaggagagta cccgtggcag tcaaggtgcg
     3601 ccgggagaag aagagcgagt tcgtcgcgaa gctcgaagaa gtgctcccgc tgctccggga
     3661 gcggttcggc gtcgcaagga tcggcatttt cgggtccgca gcccgcggag aggagcggcg
     3721 tggacgtgct ggtcgagttt gcgccaggta gatggcgttt cggaatttca gggggccggc
     3781 ggcaggcaga cggtgggaga gacccccagg ggcggccgat gggtgcctga ccgatcgcgg
     3841 gccggcggtc atcgccgggt gtgcccaccc gggaatctgc agcaccatcg tgcaggcgcg
     3901 cgcgtttacc ggggaggaac gcgtggccga tgtcatctcg aatgggcaaa ccccctgcgc
     3961 aagcgggggg tagttgaccg gtgggttcca tctcctcagt tcgcccgttg aacagatcct
     4021 ggccacatgc cgttatttcg tccacgcctg ccattgcacc ggatttcggg cagcaatcgc
     4081 gcttgcgcac gccgcacccc tgcatgaggt cggcgtcggc ctgaagatcc ggtatgactg
     4141 aaagggttcg tggaacggag gattactcat cctccggcgc accaaccacc ggccgggaaa
     4201 aatcgcggtt cgcgacgaac ttatcgccgg taataccgta gaacctcctg taaaattccc
     4261 gctgttcttc ttccaggtcg aatgaaaaga tctccgggtg gagggtggtt gcgaggaaca
     4321 tgaaccccag gatccaccgg gggctgccga aatcccatcc tgcaggcaca ttgtagactc
     4381 tccctgctcc gacggcgtcg cccacaatcc catgctcggt gcagtagtgg agatagtcgg
     4441 acgccggtga ggagaggaac cccgagatga acatgtattc tggattgagc tcgtttaatt
     4501 cgtcgacggt gatgttgacc ccgggctttc ctttccgctt caggcgcttg ttgacgcaga
     4561 tccctcccgc cgcctccacc aggttggtct cgaaccgctc cgcgttcagg gcgaagagcg
     4621 ggtagcccat cgagtagtag acgcgggggc gggttttggc gagagcactc ccggcggcga
     4681 tgcgattttt gatatataaa tataattatg tgctactgtg ctggagggtt cgcaaagtga
     4741 gttgcatcat ctcgtatgga agactgagtt caccctggct gaaagagcag cgccggaagg
     4801 gtgagaagtg cctggagatc aagtatatcc agaacaaacc ctacgtctac gaccgagcca
     4861 ccagatcacc aaagaaggtg agcacatgca tcagccatct cgcaaaaggc gccggtctca
     4921 tcccgaaagg atcacgcggc agccgtccca catctcctcg cccagggcgg tcagggtgta
     4981 tgggaacacc gctcccatgg ccgagggatt cggtgacctt ttgccatgat ccatcgtcat
     5041 acatgttctg catccctgcg gatcaccgtt tttcccatcg ccgcctcgat ccgatcaagg
     5101 atcgcctcgt atcgcaggtt atagaatgtc cagaaatcgt cgtttcggag cgcctctggg
     5161 tctataacgt gtgttcgcag gatctggtcc agacgctctg acgtgatacc ttcattcttc
     5221 tcgatacgcc gcagatagac gcttggagca ctctctccga tcatacggtt ggtgcgggca
     5281 gagagcggtg ttttattgat gatactctcc acagccatcc tgtgcttgga gagttccaac
     5341 ccgccgagat tgtcgctaca ccatttcctg gggaatatat gatggatatc gacattctca
     5401 tcgtagtagt ttgtaatatc aatcggctgg cctgaccgga agtcctcgca cccgtcgcgc
     5461 attagcagtg caaatatacc tttgtaggcc gcactcaggc gaattctcag ttcctcaagg
     5521 cgtcttccgg aaaacagggc ctctttcact gttgtcggtt catcaccgcc atggatccag
     5581 gcgaccaatt ctggcagatc ctttgcaaag cggctctcaa ctgcgcttcc atagagttca
     5641 ccgaagactc cgcaccagta ccaccgcgag agaagttgtc ttactgtgtc gttatcgtct
     5701 ttaccgccga ggatcgcaaa tgtggcggcc agcggtacta tctgcgtctg gtagggtaga
     5761 tctctggcgg tgaagatctt ctgtatcctc aggaacttgg cagccttcat gaaaccgtca
     5821 accgccgcat cacgttgctc aaggtaatca tcgagattga gtctgaggat gtctttgcgc
     5881 ttgcagttga ctgcatgcgc ccgtttatca ggatcccctc tatccatgta ttcctggttt
     5941 cgtttcatcg tcgtaaggag ggtgaccgcc tggaggaaat aatcgttagg aatctttttc
     6001 aaaacctcct ctccaccaaa tttctcctca atatccttcc agtcctcccg gagtgggaag
     6061 ttgtcggccg caaacgtggc ggtcaggagt tcaaagaccg ttaacgctac cccgccggtg
     6121 tttaccttct caaaaaccag gcagacagct tcttttggtg tctccttcgc catctcgatt
     6181 acagggagct ggtagtgctc gaatcgctgt atgacctctg catagaactg gttaaagagt
     6241 tttatctttg atttgtcata atcccagtat tcctcgtaac ccatcctcca tgaggaattg
     6301 cggtcataga tcttttttac cggaaagaga ccggcttcgt actctttttc cggtgtagag
     6361 taatcttcga tggccatgcc ccgaaagtct ctgataactt tatcctccgg aatgctccgg
     6421 atgcagtctt cgcgatcttc gttggggtcc aggcaccttt cgatatcgaa gtaatagtat
     6481 cgatgtatag gtttcttcct ggcatcctcg gtaaagaccg ggcccggttt gatcagcgcc
     6541 tgaaaaagag cggtgagccg ctgctggccg tcgagtatca actgttcggg cgcgacgacc
     6601 ggttcagagg gcaacccttc gatcggtcgc ggtttgaacc ggtattcggt atttcctgtc
     6661 tgaagtaaca tcacggtccc gatgggaaac gaaagggaga cgcttgatag cagactctgg
     6721 atccgatagt catcccagac ccatccccgc tggaagtcgg gaagttgaat cttgcgttcg
     6781 ttgatcccct taagaatgtc attaaggaag atctttgtgc tctgaaaagt tgtgattccg
     6841 gttaccattc ttcctcctcc tagtgtttga taagattgta agacgtccgc accgccccga
     6901 tctcgtactc aatgctgttc tggagcaact tctcaagtgc aacagacgac cctttgagtt
     6961 cacggggttc tgccccgtcg atgcaaaaga tgcggatgtc acccacgggt ctcaccctcg
     7021 caggtatctc ccgccgaccg ggacgaaccg ttccggcttc ttcgccgtat cgtatttcgt
     7081 gataccctcg agttcaagag ccatccaggg tctcttcctg ggctcgccct tccggacgcg
     7141 gacaccctgc caccgttcct ggctctcgac gtaggcccgg gtctcttcag gcgtcaggaa
     7201 gaccgcgtcc ccgaaggtct caaagaacgc gaacgggtcg tcgttcagga tgatccgcct
     7261 gatcgcggcc tcgccgacgt agccggtatc ttcgcgggac tggtagaaga caagtttcat
     7321 cccgctcctg agttccctgt atacggtggc gggcttgatg aagacggtct ttcccgccga
     7381 gaagaaccgg ggcatcaggt gctttgggac cgggaacgtg acgccggtga tgctggtcat
     7441 accataatcc tcctctaccc ctggctcatt atcaaccggt agtcctcttc cggtatggtt
     7501 cgcatcgcct ggccccgtat gtgccctgac cactgtttct tgttcgttat gaacgcgagg
     7561 ttcgggatga gcggtttgaa ctcgatcggc gggtcaaata tcatgaccgg ttggagtttg
     7621 acccggaggg ggaagatctc gtcgccaagg ttcgggggag cggtgaagat ccttgatgaa
     7681 tcctcataga ccgttgatgt gatctcgaag catccggtga tcgcaggcgg gagagtggtc
     7741 tccttatcga tcacctgctg cccgacgtag atgagcaggg tatcgccggg ttctgtcctg
     7801 gcaatcgtgt tgatgtgcct ctttgggaca ccccatacgt gtttttttat tacaaccttt
     7861 gaattgtccc ggttggatat tgcaagccag tgttccataa gagagacttg tcccgcggga
     7921 ataaaaaaga agtgcttatg gtgttagggg aaaagacaat cccctgatcc cgatcgccgt
     7981 tgaaggcagt ccaacccggt gagcgtatgg tctatcgttc tggggatatt gtatctttgc
     8041 gaagccgcct gtggagggtc gattcggttg atggaaagat cctctctgca acctgtatcg
     8101 agagcgacac cgcggagcag cgccgcttct acctaccgtt tgaaaaggtt gaacaggggt
     8161 ggattccccc gcccaacccg gagatatacc ggattagcaa ggtgagccca ccccgtgaac
     8221 acttacgagg ctgtcccgtc tccagcccat tgaaccccgg caatcctcct cgatcattgt
     8281 gaaaatgcgt tgaagaccgc cccgatcctg aaaaagaaaa aggatgggtt tgcggtcatc
     8341 ccgggcggaa aacccgggaa gtcccgctct cccaccattc acccactgga gcgagtctct
     8401 tcagagaagc cccaggatac cgacgatgat cgcgatgaca ccgatgatcg tctggaaccc
     8461 gcccagccac ttgccaaact tctcgaggta gacaccgagg gctggaacca catggaacac
     8521 accggtcagg aggataaccc cgaccaggaa ggcaactatg ccctgcagcg agaagatatc
     8581 cagcaatcca aggattatca tgatgatccc tatgatgacc tggaacccgc ccaggaattt
     8641 gcctgccttc tcaaggaagc cgccgagaac aggcagagcc cctataatcc ccgcacacag
     8701 caccagtccg ccaattatgt tcattatcgc ggttattgcc acattttcac ctccgctaca
     8761 ctttctttta gatacagttc ttttataaat acccttcgaa actcttcagg gcgcccgatg
     8821 aaccgccgtc ggcgtgatgg atgtggccct cctccgcacc ctcctgacgg caggagtcag
     8881 cctgataccc gatcattccc cctcagcgga ggaccggttt gaggaactcc tctcaacctg
     8941 tgaatccgcc cttgagagga atgtccttga ggcgatctat aaggcgggcc tccctctgcc
     9001 ggatggcgga caggaggtca tagcagaggg agacgagatg atcgcccgtc cggacttcat
     9061 ctaccggcgg ggcggtcact cgatagccat ctttgtcgac ggaccggatc acgaacggga
     9121 gaccatcaaa cgcgatgaca tgcaaaagag gggccggctt gacctgatgg ggtataccgt
     9181 cttatcaata ggttatcggg actcgctgga agagtgcatt cggagcttaa cggaattatt
     9241 ggagtaaaca cctgcaacac tccagaaaaa ggtccttcaa ccggtcgcgg aggaataccc
     9301 cctcctgatg atcgccgccc gccgtcttga ccggggtgcc tatggccccg ccggggaatg
     9361 gcgagaccgc cctttttcag gccccgctgc ccctgagaat tcactttatg taattgtgct
     9421 tctggagcca gtctacctgg ggcctgatgt agcggtagac gatatcgcac ttctcgtcct
     9481 ggaaactcca tggaacaacg atcaggagat ccccctcgcg gatccagagc ctcttcttga
     9541 tcttgccctt gatcctcccg gtgcgcgtga caccgtcctc gcagcggaca cggatatggt
     9601 ttgccccgag catcagctcc gcccggccaa agatctcctg gttcttcttc ttcggcagcc
     9661 ttacgcgtat gatctcttca ctctcgttct tcccgcttcc tcttggtgta gttgtcagta
     9721 aatcaactcc atgaatggca gaggcagagc cccgccagac tttatcggat cttccttccg
     9781 acttttacat aatggccccc acaagcatta aagtatcccc ctgagtggcc cggacaccac
     9841 cagccctgga gcgcaagcgg gggtggacgc cgcaggcagc cttcagatct tcctccgaag
     9901 gttagttaag gattgagcgt acataactga cgaggatctt tgaatggaaa gcaacaaact
     9961 gtacgtcggc aacctcacct actcggtaaa caaagagcag ttaaaagagt tattcggtca
    10021 gtacgggacg gtgaacgacg tcagagtcat cgagcgtaaa ggcttcggat tcgtcgagat
    10081 gtccagcccc aaagaggctg aagaagcaaa agaagccctg aacaataccg tattcgaagg
    10141 ccgcaccctg aagatcgatg aagcacggcc gcagaggccg cgaagcgact tccggcgcta
    10201 ctgaaaagcc agagagtaat atctccctat tttgagagag cggggttctc cagaaccccg
    10261 ccacccccga ttcttcggcc gggccggccg ccgccacctg gggttattgg ccgccacggc
    10321 taaaaaacca tgttaatccc cattcgggcc tgaaatctcc gtaatatcgc accagatcaa
    10381 aaatttatcc cgcgaacgaa ctgcatattt ttaataccag gaggtgttaa acaccttctg
    10441 aggcttgcaa tggagataca tgtggataag gacgcgtgcg ttgggtgcgg cctctgcgta
    10501 aaagactgcc ccatgcatgt gtacgaactc cagaacaacg taagtgttgc ggtcaggccg
    10561 aacaactgta tggggtgcct atcctgccat gagatctgcc ccgctcaggc gctcgagcac
    10621 cgcgggatct acgccgcaaa gcggcactac atcgacattc acgtatgcga gatgcttcgg
    10681 aaggtgatct gatgaccgca ggctatgtcg acgagatgca cgaggccttc cagacggatc
    10741 tggtctaccg atccgaggac gtccctctgg aatgctcacc gaagccaaaa gaccttgagc
    10801 agacgctcca cggtctgatg aaactcaacg ggctcgtgat caggtcactc gaagagatcg
    10861 cagggagggg ggcaaacgcc gtcacctacc gcgccggaaa gaagttcggc cacgaggtcg
    10921 cccagtactt ccagaaacgg gatgacgtcg agggagcgct ccacgagctc tccgaccttc
    10981 tccgggggca gtactccttc gaggtctgga aacccgagga taaagactcc tacatcatcg
    11041 aagagaacgg cgagacgttc atctacctcg tcttccacga ctgcatcgtc cgccagacgc
    11101 tccggcgcaa cggtcttgac cagggcggcc cgctctgcca gaccctcttt ggctacgttg
    11161 tcggcgcgat cgaggagatc acgggcagaa aggccaaact ggagatcgtc cacaccggcc
    11221 cgaacgcctg cctgaagaaa ctggtcctta agtgaggcga tgagcatgaa gattgctatc
    11281 gaggaactgg cgggatgttc aggctgcacg atcgcggtgc ttgacctcca cgagatgctg
    11341 ctcgacgttg ttgataaggc cgatatagtc tactcgccgg tgatcatgga cgtcaaggaa
    11401 cccccggagg gcatcgatat cgcgtttgtg acaggagccg tccgcaacgc tgagaaccag
    11461 gaacgcctgg agaagatccg gagacgggca aagaccctgg tcgcgctcgg cacctgcgcc
    11521 tgttacggcg gcgtatcggg tctctcaatg ctgcactcaa acgatgagat cttctcatac
    11581 gtctaccggg gggtggagac cgtgaaaccc gacggggtca tcccgacgga tgtgcccccc
    11641 ttcctctacc gggcgtttgc ggttggcgac ctgacaaaga tcgactacta catcacaggc
    11701 tgcccgccaa aagagatcta ccttaaacag atcatccccg cgctggtctc cggggacaaa
    11761 ctcaatctct cgcgcaaatc cgtctgtgcc gattgcgaca gaaagatggg cacggtcgag
    11821 aactggacac tcaaacgccg ctacgagggt gtccccgacc gcgaacactg cctgctgggg
    11881 cagggttacc tctgtctggg gagcgtcacc ttcggaagat gcggcgccgc ctgcccgaag
    11941 aacaacgtcc cctgccacgg ctgcaacggc ccgtctctgg acatcctccg cgaaccctgc
    12001 cgcgacatct acggcatgat ggtgcggagg atctccgatc tcaccgataa gccccaaaaa
    12061 gaagtggaga aagaactcta cgatatagcg cacacgatgt acccattcac catcggcagt
    12121 ctgatcatgg aggacaaaga ggtctcaaag atccgcgacc tggtaaagga gaggagcaga
    12181 tgaaagaggt cacgataaac ccggtgaccc ggatcgaggg tcatgcagac gtcaggatat
    12241 acctcaacga ccgtggtgaa gttgactctg cccattttca ggtggtcgaa ctgagagggt
    12301 ttgagaagtt cctcagcggg gctgcaatcg aggaggcacc caggataacg ccccggatct
    12361 gcgggatctg cccgacctcg caccaccttg cggcggcaaa ggccaccgac cagatcttcg
    12421 gggttgaacc ccccgccaca gcaaagaaac tccgcgaact cctcaacctc gggcagtaca
    12481 tccactcaca ctccctccac ttctttatgc tcgcggctcc cgacttcatc ctgggccacg
    12541 actgccctcc ggagacgcgc aacgtcatcg gcctcgcccg ccactcaccc gatcttgcaa
    12601 agaaggcgat cacggtcagg aagtttggcc agcggttgac ggaggcgatc ggcggcaaac
    12661 ccatccaccc ctcaaacgcc ctccccggcg ggatgtcggc accattaacc gcggcaaaga
    12721 gggccgaact ggttgccatg gcggatgagg cgctcaggat cgcccttgaa ggctgggata
    12781 tcgcgcgcac actcctcgac ggtgtcgaca cggagttcgg cgccgtcagg accgggttca
    12841 tggggatgac caatcacggt gtctactcga cttacgacgg gcctgttgcg gtgctcaaca
    12901 gggagggtgg aaggatcggg tcgttcaccg gagaggatta cacaaaattt atcgaggagt
    12961 attcagtgga ctggtcatac ctgaagttcg tccgcctcaa gggcggtgag aactaccgtg
    13021 tcgggccgct tgccaggctg aacatcgtgg agaagatggg cacggagcat gcagacgctg
    13081 cgcttgcgga gtatcgcgag cggttcggaa cagtctcaca ggcagcgctc gcctacaacc
    13141 tggcgcgcta cgttgagttc atcgcctcgt gcgaacgagc ggtgaaactc ctctccgata
    13201 gcggcatcac cggcacggat gtcaggacac ccgcaggaga ggttgtgaac cggcgcggtg
    13261 tcggtataat cgaggctccg cggggtacgc tcatccacga ctacaccgtc aacgatgccg
    13321 gaatcattga acggtgcaac ctgatcgtgg cgacctgcca gaacaactat gcaatagacc
    13381 gcggggtgga ggatgtcgcc cgccgggtcg tgaagaacgg tgcactgagc aatggtgcag
    13441 ccaaccagat cgagatggtc ataagggcgt acgacccctg tatctcatgc gccacgcacg
    13501 cgatcgggag gatgcccatg aagatcgagt tagtcagaaa ggcgtgaggt gatgtaaagt
    13561 atggaaagca tgttaaagca gatcctggga gagttcctta aacttgatgg ggttaccgcc
    13621 gcggtcgtgg cgggacgtga cggttttgtg atcgagagtg ttgttgccgg tgacgtggat
    13681 gtcgaggccc tgggcgccat ggcatcgaca ggcatgggta cgtccgaggc gatgagcaat
    13741 gaactcggga agggagagat gcaccagatg cttgtcgagc ttgagaacgg cccgattctc
    13801 ctctcaccgc tctcggagga tgagttgatc gccatagtgg cgaacgcgaa cgtaaacgtc
    13861 gggagaatcc gctacgaact gaagaagaac aaggaccgaa taatagccgc tctgtgagac
    13921 gatgctcccc gacggcatct cacttggcag actgcagggg cagctctcga cccttgcatc
    13981 gatcagccct catttcaccg gggcggtgcg gatcacggag aagggtggtg gaaccggctt
    14041 tgtcctgttc gaggccggga acccttacgc cgccgccttc gaggataaaa gcaggggtct
    14101 cctcctcggc ggagatgacg cctacaggca cttcctcgag cggccgtcgc tggtctacga
    14161 gcttatcgcg tacaccgccg aagagacgcg ggcggccagg gaggcttcga gtgcgagcgg
    14221 ttgccttgtc accggggttg gcagagcgcc cccctccccc gagaaggctg ccctccagca
    14281 gatcgtgcgc cagccgggcg tcgttgcggt ctcggtcttt catgaaggat ttgccctcca
    14341 gtcggtcggc tctgcggact ttgagaaggt ggccgcggtc gccgaggatc ttctccgtgc
    14401 cggaagccag atcaccgccg agatggagat gggcggtctc tcccaggtaa tcctcgagac
    14461 accccggggg aagttcatca tcgcgcccta caacgacctc tacctctgca tacttgcaga
    14521 accggatgca aacctcggcc tgatccggct ctcgatacgt gggatgcagg agaaccgcgg
    14581 gtaaagaccg gattgttgat atacccctcc agcggagcag gtttatggtt ctccagatcg
    14641 gaaccggggg cgtgcccctg cctctatttt atccggttgg cagcacgggg tgatcacccc
    14701 gtgaagtatt accctgttca ttccggctgc ccgccgtata cggcggtgga ccgtgtgatc
    14761 gagtcggcct ttgccgagca cggcaggggc aacgtccaga tgcccccgaa ggtctacgtg
    14821 acgttcggga agaccgggga cttcaggacg atgccggcct acctacccgc ggccggtata
    14881 gccggggtga agatagtcaa cgtccacccc aacaacaggg cgcacgggct tccaacggtc
    14941 atggcgctga cggtgatcat cgacgtggat accgggctcc ccacggcgct cataaacgcc
    15001 accgacctga cccgcgcttc gaaccggggc ggcaggtgct gtggctgcaa gacacctctc
    15061 cccccggcgg tcctccctca gcccggggct cataggtgcc ggacggcagg cggcggcgca
    15121 ggttgaggcg atggccgccg ccttcacgct cgacgaggtg agggtccgga gccggaacga
    15181 gaagactgca gcggcgcttg cggcgcggta tgccgattac aacgcccgcc cggtctcgat
    15241 cgagcgtgcc tgcgactccg acatcctcgc cgcgacaaca ccctcgagag aacctctggt
    15301 tatggccggg tgggtccacg aagggacgca catcaacgct atcggcgcag atgcccccgg
    15361 gaaacaggaa cttgaccccg cggatcctcc tccgggccga ggtcttcgtc gacgaccgcg
    15421 aacaggcgat ccactccggg gaggtcaacg tcccggtcag caccgggctc tacgacccgg
    15481 ccatgatcgc gggaaccctg ggcgaggtgg tcatcgggaa gaaggggagg tcgtccccgg
    15541 acgcgatcac cgtctttgac tcaaccgggc ttgctgccat acaggatctc gccattgcat
    15601 cgcttgtcct ggaggatgtg gaaggctgcg aactgccgtt tccgtgatcc ttgagtcctt
    15661 cctgcggggg tgggcttccc tcactcatct tcacggggct gctgcgcaaa cttcttccag
    15721 acctgctcgc ggtggaccgg gccgctgttg tatgtgcaga atggtatcag gcggccgtca
    15781 ggggtagcgt agtggatgca gcaccgctcc accctggaga ggtcgtagtt gtatctgtcc
    15841 atgaagtgca tcatgccgat gaagagggcg ttccagtgga agtcacgcag cgcctcgaag
    15901 ttctgcagca caagcgcctt cattataagt ttcatcaact ccccggtgtt cttgtattcc
    15961 gccttcctgg tggatgtgta gatgtctttg accccctcga caagggtcac gtacttgttg
    16021 agcgacccgc ccttcgcaag ccttgccgcc atcttctcga ccgactcaaa gaaagcgtcg
    16081 acatccacca tcctgttgat cgggatcata ccctcatcgg tgacaaagac gtatgtcgcc
    16141 gcaccgcagt gctggtgggt ggtgaacctg atctggggtt tgcccgtgta tgcctcgacg
    16201 agatcggaga ttggaacgac gcagggcacg ggatagaagt ggtctttctt gatgataccg
    16261 tctgtctgct cctcgatccg ctccgcaagt tccgggatgg tgatccgctc cttcctgaca
    16321 tcatccgcgc ttgcggcgcc ggtgaacgcc acaggctgaa agttgacgcc gcggatggtc
    16381 tcgaaatgtt ctgcggcgta tttgatgatg gcgccagcct cgtgatcgtt cctgcctttt
    16441 atgacagtcg gcaccagcac aacgcccatc ccgatcttct cgcagttctc gatggcacgc
    16501 agatcgctcc caagtttgga gttcgtctcg cgggttaagc cgtcgaagtg gaggtatacc
    16561 gttgagagtc cggcattctt cagctcctgg acataatcaa cgtcccgcgc cagtcgaatc
    16621 ccgtttgtag cgacctgcac ctgggagatc ccgaactctt ttgcaacgcg tatgatctct
    16681 ggcaggtcgt cccgcagcgt cggctcgccg ccggaaaact ggacggcagg ggccggaaca
    16741 ggtttctcct cgcgcagcat acgcagcatc cccacaacct gttcaaaggt cggttcgtag
    16801 acgaatccac acgctctggc gtttgcaaag cagaaatcac agtccaggtt gcaccggttc
    16861 gtcagatcga tgtttgcaag cagggtcgtc gaccggtggt ttgagcagag cccgcagtcg
    16921 ttcgggcacc ctgtcgcgga tggagtcttc tgcgggtttg aaacaccgtt cccgatacgc
    16981 tcgtaggcat cgaaccgccg gtacatatcc gcgtccgacc agtagagacc ccggtataaa
    17041 ccgtgttcag ggcaggtgcg gaccagccag atctgcccct cctcttcgac gacatcggcg
    17101 tcgagcacac gaccgcatgt cgggcagagg ctctttgtct tcttcacaaa cattctctca
    17161 ccgtgcccat tttttattcg tatctcatac ttcgacactt taatctttat atttactgat
    17221 tgtacagcac attgacattc atctcacctg agatcggcta ctggatcttc tctgcggttg
    17281 cagcactctg gatcatgctg ccggcatacc tgcccaactc agcggccgcg gtcttcggcg
    17341 gcgggacgcc aatcgacctc ggcaggacgt tttccgacgg gagaagggtc tttggcgacg
    17401 ggaagaccta ccgcgggttc ttcgggggcg tcctatcggg tgtgctggtg ggtctgatcc
    17461 agatcctggc agcctccgcg ttcggcctcg atatgctccc ccgacatacg atcctctcgg
    17521 tcatcctgct tgcgaccggc gcactcctcg gggatctcgt caagagtttc ttcaagcgca
    17581 ggcttggaaa agagcggggt gaaccctgga ttattgccga ccagtacgac cttgttatcg
    17641 ggtcgctgct cctcgtcctc ctggtatacc cggagtggct ggtggagaac atcaccctgc
    17701 cgatcctggt ctggataatc gtactgacac cgctcctcca ccgtgtggtg aacattatcg
    17761 ggtattatat aggagttaaa gaggtaccat ggtgaaccgg atcgcaagac tgctgcttga
    17821 gagcggggca atagagtatg gagactttct cctggcttcg ggtgcccgga gttcctacta
    17881 catcgatatc aaggccgcaa ccacgaaccc cgccatactt gcggagatag ggaaggccat
    17941 cgccgaaggc cgggagttcg aggttgttgc cggggtggcg gtcggcggtg ttccgatcgc
    18001 cgtcgcggtc tcgcttgcaa gcggccggcc ttacgcgatc gtccggaaag aggcaaagga
    18061 tcacggaaag tcgggcagca tcatcggcga ggtgaacggc aaggacgtgc tgcttgtcga
    18121 ggacgtgaca acctctggcg ggagcgccat ttatggcatc gaggcgctcc gggccgcagg
    18181 cgcgcacgtt gaccgcgttg tgacagtcgt tgaccgggag gcgggcgctc gtgaggcgct
    18241 cgcggagaaa ggggtctctc tccaggctct tgtgcgggtc agcgacctgc tggatggata
    18301 aagcattttt aagtgcgggt aaatatgatg gatatgaaac tgctcgttgt aggcggtggt
    18361 ggcagggagc atgcgatcac cagggcgctt tcctgcaaca gtgatgtgag catcttctcg
    18421 gtcatggcac agaagaaccc ggggattgcc aggcttgcag agcgggtgct ccttgagaaa
    18481 gagacgaata ttcagaggat actgccgttt gccaccacct gcggggtcga tgccgcgatc
    18541 atcggcccgg aagcgcccct tgaagcgggg atcgtcgatt acctcgagtc cgccgggata
    18601 ccctgtgtgg gccctacccg cgctgccgca aggatcgaga ccgacaaggc gttctgccgc
    18661 cggttgatgg agaggtacgg gatcgagggc tgcccgtact accgggtctg ccacgacccc
    18721 gaggaggcgc gccgtttcat cgagtcttac ggcggcgacc ttgccatcaa gcccatcggc
    18781 ctcaccgggg gaaagggtgt caggatcatg ggcgagcatc ttgatgcgca gggggcgatc
    18841 gattacgtac ggcaccttgg aggaaacgtc gtcctggagg agcggcttat cggggaagag
    18901 ttcaccctcc aggcgttcgt cgacggcgag catctggtgc cgatgccgct tgtccaggac
    18961 cataagcggg catacgaggg tgatgtcggg ccaaacaccg gcggtatggg gtcatactcg
    19021 ctcccggacc acatgctccc gtttgtctcg cggccggatt acgagaaggc tctccggatc
    19081 atgcaggata cggtcgcggc gatgcgtgcc gaagggacgc cataccgcgg gatcctgtat
    19141 ggccagttca tgaacaccgc tgacggcccg aaggtcatcg agttcaacgc caggtttggg
    19201 gaccccgaag cgatgaacgt cctctccatc atcgagtcgg actttacgga gatagtccaa
    19261 cggatcatcg atggcgacct atcatcatcg gacgtctcgt ttgcgaataa ggcaacggtc
    19321 tgcaagtatg ttgtcccgga ggggtatccc gaggcgccga aggtgggcga accgatcgtt
    19381 ctcggggagt atggtgatgc cctcctctac tgcgcaaacg tcgaggagag gggcggtaga
    19441 ctctatacca ggacatccag ggcgcttgcg tttgtcgggg tgggcgagac gctggaagag
    19501 gcggagtcga tagcggagag ggcagcatca tccgtctcgg gccgcgtctt tcaccgcagc
    19561 gacataggca ccagggagag cctggagaag cggtgccgcc acatgaggga gattgcatga
    19621 agaaagattt tctatcgatc ctcgatatgg agagcgacga acttgagggc atcatcgccg
    19681 acgctatacg cctcaaacac ctgaagtcgg acggggttgc acacgaatac ctgaggggga
    19741 agaacctcgg gatgatcttt gagaaggcgt ccacccgcac ccgggtctcg ttcgaggcag
    19801 ggatgtccga cctcggcggg catgcgctct tcctgaaccc gcaggacatg cagcttggcc
    19861 ggggcgagga gatccgggac acggcacggg tgctctcccg ataccttgac gcggtgatga
    19921 tccgtgccta ctctcatgcc acaatcgagg agttcgcccg ctactccacg atcccggtca
    19981 taaacggttt atctgaccgg ctgcacccat gccaggttct cgccgatatc atgaccctcc
    20041 gcgaacggtt cggcgatctc cgcggcctca aactcgcctg ggtaggcgat gggaacaacg
    20101 tctgcaacac atggatcctc tccaccccac tgaccgggat ggaggttgtg gttgcaagcc
    20161 cgccgcgcta ccgccccgag gacggggttg ttgagcgggc gagggcggcc ggcggccgtg
    20221 tcaccatcct caccgagccg gaggaggcgg ttcgcgacgc agacgtcctc tacaccgata
    20281 tatgggtctc gatgggtgac gagcatgaat ctaccgaacg gctgcaggcg ttccagggat
    20341 acacgatcga ctcccggctc ctggaactgg cggtgccggg ggctcctgtt atgcactgcc
    20401 tccccgccca ccggggggag gagatcaccg atgacgtcct ggaaggcccg cagagcatcg
    20461 tctgggacca ggccgagaac cggctccacg ctcagaaggc gctgcttgtc tggctgatca
    20521 ggggcgggat gccgccggag gggtgaggaa ccgttctact cacgggcttg gtggggcggt
    20581 ctcaccgggg gccctccgtg atcctgtttt cgccccgcac acggccaccg gatcaatccc
    20641 cagacagttg cccgccccgt cgagaggggg aggaggcggg gctggtcaag ccgcaaaaga
    20701 cgtctaagcg cgctggtggt ttttcgg
//
LOCUS       DAOSZX010000019        19162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2366_length_19162_cov_22.444183, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000019 DAOSZX010000000
VERSION     DAOSZX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19162
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2366_length_19162_cov_22.444183"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..729)
                     /locus_tag="PK196_02985"
     CDS             complement(58..729)
                     /locus_tag="PK196_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40420.1"
                     /translation="MSRPVIELNDVRKVYTLPSGDVTALNGVDLRIDEGEFVVIMGPS
                     GSGKSTLLNQIGCLDRPTSGDLFLSGKNTREMDDHDLTVLRLTSIGYIFQKFNLIPLL
                     TAYENVEYPFIMKNRRPDDTGRVRHLLTMVGIDEALSAHKPGELSGGQQQRVAIARAL
                     VNDPAILLCDEPTGNLDSQTSIQIMEILADLHLRGRTIVMVTHNPETAAYADRTITIR
                     DGRIA"
     gene            complement(745..1986)
                     /locus_tag="PK196_02990"
     CDS             complement(745..1986)
                     /locus_tag="PK196_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HON40421.1"
                     /translation="MRRNLCVLLLFALLVGAASAAETTPAEQIAVTSVTLDPETLMRG
                     DTGTVTVEIQNTGDTGVAISRAELYSSGVVVVNDRTYDSVGTLGPGNTISFTFTVRAD
                     AEDGIYYPVFYLDLRDGGSVRYSIPVTVESTGIQVNVIDAPETFPANSEDAIVLSVGN
                     PRGTSVSGVTVTPSGEGVRTTETAVFLGALAPDEEKTATFSVVASQSTELTFDLSYRN
                     GINEHHTTLTIPIEIGDRVVEPDMVVNNIEVTGSGSTITLSGDVTNAGLKDAYAVKVT
                     VDDPAAAADPYPVYVVGGLEPDDFSSFEVTCTVPQGASSVPLLVQYRDEDGKTYSTTV
                     SVSLSSAGQTLQAGTDTENPGSMSAAAPARGGMGAAGSFGSGFAKIPVTGIVLVIAVI
                     AVGTVAWRKGYVGKIRARLHR"
     gene            complement(2967..3167)
                     /locus_tag="PK196_02995"
     CDS             complement(2967..3167)
                     /locus_tag="PK196_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON40422.1"
                     /translation="MKNRIKVYRAMHDLTQEALANNLGVTRQTILAIEKGKYDPSLEL
                     AFKIARYFGASIEEIFIYDEPV"
     gene            complement(3151..3654)
                     /locus_tag="PK196_03000"
     CDS             complement(3151..3654)
                     /locus_tag="PK196_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HON40423.1"
                     /translation="MLKIIYSGCTMKRNTFYLLAGIVALAEVGIFWLSVRLENPIPIQ
                     VAFVLGVILLYLARRRVEETIEDERTAMITQKAALRTLEASWVIFFVLSLGSAVIAFS
                     RPLGLRPPHPEPHEVFPTEALELPVFGGFAIFQMTLLCLMIFLYVGFRVYYARKYGEW
                     DTDEEQD"
     gene            complement(4591..6279)
                     /locus_tag="PK196_03005"
     CDS             complement(4591..6279)
                     /locus_tag="PK196_03005"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HON40424.1"
                     /translation="MTETDIEHLLFVYALQNAVKHDSPPKTGTVIGTLLGRHPEFRSS
                     AREIGAIASKVAAEVAGMSSSERRARLEELAPELLAELDEEHEHTRELPPLEDAEKGV
                     VMRFAPNPSGPLHLGHARASILNDYYVRRYGGRYILRIEDTDPRRVDPAAYAMVLEDV
                     EWLGLGITDIVYQSDRLDVYYDWCRRLIEIGGAYVCTCEPERFRELKLRGRECPCRKQ
                     GIEENLELWQKMLDGEFYEGGATVRVKTDLGHPDPAMRDYSAMRIVNSPLHPRTEATV
                     FPLMNFSVAVDDRLLGITHVIRGKDHIANTQRQRYIFGYFGWRPPMYYHYGRMGISGV
                     VLSTSGMREGIRNGTYTGWDDIHLGTLRAIARRGIEPEAVRNAMIDIGMGETDISFSW
                     ENLYARNREIVDPKANRYFFVPDPIEVSIEGAPRREAHALLHPNDPARGVRTLVTDGR
                     VLLPRRDIEGQGLIRLKDLYNVRVDREGDTLRVSYAGDSLDEARQVKAPIIQWVPVDA
                     NLPCTLLRQEGNLEGFCEPLVVGEVGRVVQFERIGFARIDSVKSGRVNAYFAHR"
     gene            complement(6284..7249)
                     /locus_tag="PK196_03010"
     CDS             complement(6284..7249)
                     /locus_tag="PK196_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HON40425.1"
                     /translation="MMILKEYLEMNADRIDRMLHRYFGNVHGGLFRASAHLLLAGGKR
                     LRPAVTLLAADAIRKGSSDDLILAALSLELTHNFTLIHDDIMDGDTARRGVPTVHTLW
                     DEPTAILAGDVLYAKAFEFICLAEGADAAKIRAVKMLARTCAEICEGQSMDMEFERRE
                     DVSELEYLEMVSKKTGALYGAAAAIGGLLAGANPVQTDALYQFGINSGIAFQIQDDLI
                     DLLADTEESGKDRASDIREGKQTLIAIRAREKGIDLAPYRRPLSAEEIEGLVTMLRDA
                     GVIEEVRAVALERAGVAKEALSVIPESEEKQLLGEIADFFVNRGN"
     gene            complement(7260..8597)
                     /locus_tag="PK196_03015"
     CDS             complement(7260..8597)
                     /locus_tag="PK196_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HON40426.1"
                     /translation="MDIEIITVGGFNEVGRNMTAVRCGKEIVIFDMGLRLDQVMIHED
                     ADIENMHSLDLIEMKAIPDDTIMNTVEGSVKAIVCSHGHLDHIGAVPKLAHRYDAPII
                     STPYTTELIRQQIAGEQKFGVNNKLFALKAGQRYTISPHLTIEFVRSQHSIIDTVIPV
                     LHTPEGAVIYANDFKLDRTPVLGEPPDFARLRQIGKEGVLALVTECVNIADKGRCPSE
                     KIARDLVRDTITSYEDDKSALIVSTFSSHISRIKTIAECAHEIGRKPVLLGRSMERYA
                     TAAEQLKLVGFPESLSIFGNRRTVDRTLRRIIKTGKDKFVPIVTGHQGETGAILTRMV
                     MGDTPFKLEKGDKILFSARVIPNPMNRGQRYLIEARARMAGARIFDDLHVSGHAYRED
                     HYEFVHLLNPQHIIPSHGDIDMTGEYVNFAEEVGYTLGNDLHVLRNGQNLRIN"
     gene            complement(8587..9675)
                     /gene="fni"
                     /locus_tag="PK196_03020"
     CDS             complement(8587..9675)
                     /gene="fni"
                     /locus_tag="PK196_03020"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HON40427.1"
                     /translation="MRRDTATSSRKRDHLEICYEQPVEAGDAGFGDVRLVHNAIPECD
                     MDAIDLKTRFLDAKLDSPLFIAAMTGGHPDTLEVNRRLARAAERYNLGMGVGSQRAAL
                     ENPELEGSFTVVREEAPHAFLCANLGIIQLRDHGIEWAERAVEMIDAQAIAIHVNLLQ
                     EAIQPEGDHSAVGCLEALRGLCEEFSRPVIVKETGSGISIGTARKIFGAGASAIDIGG
                     YGGTSWAKIERLRATAPELAELGEAFLSWGIPTVVSLCEVRRAGGPTIATGGLRSGID
                     IAKSLALGADLGGMALPLLKPAMESEEALSATIEGMHRHLRVAMFLTGSRTVADLRHT
                     RTYITGLTRQMIESNDKTKDPARRLHGY"
     gene            complement(9679..10437)
                     /locus_tag="PK196_03025"
     CDS             complement(9679..10437)
                     /locus_tag="PK196_03025"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HON40428.1"
                     /translation="MTERVVLKLGGSVITDKSGGCTIDHARLREIAEVLAAHREIELV
                     LVHGAGSCGHPEARRYRVDEGLGRDNLPGVYHTHAAVSRLNAAVVEALREAGIEAIGI
                     HPLCLSLAEDGRLVSFETRQIAGMTDHGIVPVLHGDVVMDTRRGSCIVSGDQVVARLA
                     LALGSRRVGLATDVAGVLRGGSVIPRIDREILESLDVGGSAKTDVTGGMRGKIAELLI
                     LADAGIDSEIFHVSQIGRFLDGTDHGGTAITRKE"
     gene            complement(10430..11299)
                     /gene="mvk"
                     /locus_tag="PK196_03030"
     CDS             complement(10430..11299)
                     /gene="mvk"
                     /locus_tag="PK196_03030"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HON40429.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPAIAMAIKPRVFVTVRKSRNPT
                     RPKSPYISECFKVMGVRGSVYVRSQLQSSSGLGSSAAVTVATLSAINDEFDLGHPREY
                     IAETAFAIEKKVQKGRASPTDTYVSANGGMVMITGESKRRLPPENLHLVVGNTMVPHS
                     TAKMVEQVANLRQHHPAIANPILDAIGELTLAAIHEMSNPKELGQYMDMNHALLEALG
                     VGHPASSRLVLAARASGAYGAKITGAGGGGSIIALCPRRAKNRVAGAIEASEGRAIIT
                     TIDTDGAREEKND"
     gene            complement(11301..12089)
                     /gene="amrB"
                     /locus_tag="PK196_03035"
     CDS             complement(11301..12089)
                     /gene="amrB"
                     /locus_tag="PK196_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HON40430.1"
                     /translation="MDMRRCSVAGMFYPAEPSHLEQVLEAFFRNKNPDISAHGIVSPH
                     AGYIYSGETAACAFSTIQPDFDGTFVVIGPSHHGYMTSASAMPWETPLGVINVDKEFV
                     DALDLRIDETSHRSEHSLEVQMPFIRYRFPRARIAPILMGDQSYEAAMNLAEHLIDAI
                     GRTRRDVRIVASSDFSHYVPEVVARRQDLHVIEALKTLDIPEFYRRIIATRATICGYG
                     PIAAMCTVCRHLGGRRGALLRYTTSGDVTEDYNQVVGYAAIAVV"
     gene            complement(12116..12724)
                     /gene="rpsB"
                     /locus_tag="PK196_03040"
     CDS             complement(12116..12724)
                     /gene="rpsB"
                     /locus_tag="PK196_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HON40431.1"
                     /translation="MTGNELEIELKEPLVSVEDYLAAGVHIGTQQKSKDMMKFIYRVR
                     GDGLYILDIQATDDRIKTAAKFLSQYDPAKILVVTSRQYGQYPAKMFADAIGGMAVIG
                     RFIPGMLTNQRLNKYIEPDVVVVTDPISDAQAVNEAVQTGIPVVALCDTNNMTKYVDL
                     VIPTNNKGRKALSLIYYLLTKEMLRLRGVTTSLTPEDFETEL"
     gene            complement(12726..13913)
                     /locus_tag="PK196_03045"
     CDS             complement(12726..13913)
                     /locus_tag="PK196_03045"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HON40432.1"
                     /translation="MTTIEEITLRTILDSRGNETVEADIYTSRGFGRAAAPSGASTGT
                     YEAKVRPPREAIENAREHLIPSLIGEDACDQITFDALLREIDGTPYFSSIGANVAVAL
                     SLACAKAAASSLDLEVFRYLGGAFASETPLPLGNIIGGGAHAPNATSIQEFLVVATGA
                     SGATEAVFVNAAVHKAVKKILQAQGKLSGKGDEGAWAPAITDAEAFEILGEAIGAISD
                     EMNVEIRMGIDMAATELWDGEHYRYRDAVRNRDDQIAYAADLVDRYNLVYVEDPFFEE
                     DFEAFASLTEEVGDRCLVCGDDLFVTNVERIIQGIETGAANCVLIKPNQIGTLTDTFE
                     AVRLADENGLETVMSHRSGETTDATIAHLGTAFGCIFLKTGVVGGERIAKLNELIRIE
                     ELI"
     gene            complement(13918..14088)
                     /locus_tag="PK196_03050"
     CDS             complement(13918..14088)
                     /locus_tag="PK196_03050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HON40433.1"
                     /translation="MESYTRYERARIIGARALQISMGAPVLVKTRKTEPLEIALEEFE
                     RDVIPITVKRKL"
     gene            complement(14119..14192)
                     /locus_tag="PK196_03055"
     tRNA            complement(14119..14192)
                     /locus_tag="PK196_03055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14155..14157),aa:Pro,seq:cgg)
     gene            complement(14193..14381)
                     /locus_tag="PK196_03060"
     CDS             complement(14193..14381)
                     /locus_tag="PK196_03060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HON40434.1"
                     /translation="MIPVRCFTCGKVISTAWKEFKERRDAGEDPKQILDDLGLERYCC
                     RRMLLTHKEAVEDLNPYQ"
     gene            complement(14416..14817)
                     /locus_tag="PK196_03065"
     CDS             complement(14416..14817)
                     /locus_tag="PK196_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HON40435.1"
                     /translation="MVKIVNSSGKRKTAIARATLKPGNGRIRINSVPLEIYGTEVIRM
                     KIAEPLLLVPDALDGVDVAIEVSGGGRMGQAEAVRTALARGILEWHNDPQIKDAFLSY
                     DRTLLVNDSRQKETKKPQGPGARAKFQKSYR"
     gene            complement(14832..15254)
                     /locus_tag="PK196_03070"
     CDS             complement(14832..15254)
                     /locus_tag="PK196_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HON40436.1"
                     /translation="MVQVIDADGLLLGRMASIVAQRALAGEEIAIVNAEKAIVSGSKA
                     HVLANYTMKRERGSREGGPFFPRRPDQIVKRTIRGMLPYRRERGAAALGRVRVYVGIP
                     AEFSGMDMETLEAAHINRLSSPRYVTIGTISTKLGAKY"
     gene            complement(15260..15628)
                     /locus_tag="PK196_03075"
     CDS             complement(15260..15628)
                     /locus_tag="PK196_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HON40437.1"
                     /translation="MTKTTANKSNPRLTALIATLKEASRVNEANIWREIAKRLDAPRK
                     NYAEVNLSKINRYANKDEIILVPGKVLGSGALSLPVKIAALDFSETAVTKITSARGTC
                     MTIEDLIRDNPKGSRVRILR"
     gene            complement(15628..16455)
                     /locus_tag="PK196_03080"
     CDS             complement(15628..16455)
                     /locus_tag="PK196_03080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HON40438.1"
                     /translation="MEIAFSRLDERVAKFTLSGVSTSFANIFRRAMMSEVPTLAIENV
                     RIYDNTSVLFDEMLAHRLGLIPLRTDLEQYRLRSECTCGGAGCQFCTATYTLSAEGPK
                     TVYSSDLIPQDPDAAPAEENIPIIELSNDQKVVLEAQAILGTGKEHAKWQATTACGYK
                     NYPAITIDKRCDGCGMCLEECPRSVLEAAEGRVRVVNGRLESCSLCRLCERACLAGGI
                     GDEPAIHIGTDPEKFIFVVESDGSMPVGEIIERALQYIQKQSDDLVDVMNEITGEGM"
     gene            complement(16455..16844)
                     /locus_tag="PK196_03085"
     CDS             complement(16455..16844)
                     /locus_tag="PK196_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HON40439.1"
                     /translation="MAEEKWGIAHIYASFNNTIITITDLSGAETITKSSGGMVVKQDR
                     NESSPYAAMQMATQVAQNAREKGITGLHVRVRAPGLGKQRSPGPGAQAAIRALARAGM
                     KIGRIEDVTPVPHDSIRSKGGRRGRRV"
     gene            complement(16849..17397)
                     /locus_tag="PK196_03090"
     CDS             complement(16849..17397)
                     /locus_tag="PK196_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HON40440.1"
                     /translation="MAYPGKNHKQYATPKRRFEKTRLDDEKRLVIDYGLRNKRELWKA
                     QSILRKYRAAARELVALRSAGITSEIYQTKKDQLISHLYRYGLVGENADVGDVLALKV
                     EQQLDRRLQTQVLRKGLARTPKQARQFITHGHIAIGGQRVTVPGYRVTREEEPEIGYY
                     EVSPLTNEVHPERGRITRAGVR"
     gene            complement(17409..17858)
                     /locus_tag="PK196_03095"
     CDS             complement(17409..17858)
                     /locus_tag="PK196_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HON40441.1"
                     /translation="MDDEEIKYFVRIRNTDLDGTKTVQIALTGINGIGHHTSRTITAL
                     AGVDPHAVLGKLDDESIRRISDAVETYTTQVPEWMVNRPKDVYTGEKRHLLGTDLSLA
                     IEDDINRMRKMRCYRGIRHETGQKVRGQRTKSTGRTGMTVGVRRKKI"
     gene            complement(17893..17977)
                     /locus_tag="PK196_03100"
     tRNA            complement(17893..17977)
                     /locus_tag="PK196_03100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17941..17943),aa:Ser,seq:gct)
     gene            complement(18234..>19162)
                     /locus_tag="PK196_03105"
     CDS             complement(18234..>19162)
                     /locus_tag="PK196_03105"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HON40442.1"
                     /translation="TALLESMSMTGFQGRKLGESLGVWTRMITDPDCTIFMGLSGAMI
                     PAGMQNVLIDLVRHRYVDVIVSTGANIFHDTCEHLGVRHYIGHQHVDDAALFEEGIDR
                     IYDVFAYEEEFRSVDAEIARFAGSIAPFQGSSREFTRRLGGWLRENRPEGQSLVATSA
                     ECGVPIFIPALCDSSIGIGLVMARRRGVNIDIDQLADTDEITRMVEGARKTGVIYIGG
                     GVPKNFIQQTQVIASIHEQNLGGHAYAIQYTTDAPHWGGLSGCTFEEAISWGKEAPES
                     HRVQCFCDATIALPIVASGLIGSGVKRARRAP"
BASE COUNT         3442 a         5885 c         5541 g         4294 t
ORIGIN      
        1 gcataaagtg aagccggacg ttccttgctg cgagcgtgag gatgatgtct ttttttatca
       61 tgcgatcctt ccatccctta tcgtgattgt tcggtcggcg tatgcggcgg tctccgggtt
      121 atgggtcacc ataacgatcg tccgccccct caggtggagg tctgccagga tctccatgat
      181 ctggatgctc gtctgcgagt ccaggttccc cgtaggctcg tcgcagagga gaatcgccgg
      241 gtcgttgacg agcgcacggg cgatagcgac ccgctgctgc tgccctccgg agagctcgcc
      301 aggtttatgc gccgatagcg cctcatctat cccgaccatg gtgagaagat gccggaccct
      361 ccctgtatcg tcgggtctgc ggttcttcat tatgaagggg tactcgacgt tctcgtaagc
      421 ggtaagcagg gggatgaggt tgaacttctg gaagatgtag ccgatggatg tcaaccggag
      481 aacggtcagg tcatggtcat ccatctctct ggtgttctta cccgaaagga agaggtcgcc
      541 cgatgtcggg cggtcgagac agccgatctg gttaaggagg gtcgacttgc cagaccccga
      601 gggccccatg atcacgacaa actccccctc atcgatcctg aggtccaccc cgtttagcgc
      661 cgtgacgtcg cccgaaggga gggtgtagac ctttctgaca tcgtttaact cgataaccgg
      721 cctgctcatg cttttacccc gggattacct gtggaggcgg gcacggatct tgccgacgta
      781 tcccttccgc cacgccacgg tgccgacggc gatcacagcg atcacaagca cgatcccggt
      841 gaccgggatc tttgcaaacc cgctgccaaa cgacccggcc gcacccatgc cacctcttgc
      901 aggggcagca gcgctcatgc tgccagggtt ctcggtatcc gtgcccgcct gcagcgtctg
      961 gcccgcactc gatagggaga cgctcacggt tgtgctgtag gttttgccgt cctcatccct
     1021 gtactggacg aggagcggaa cggaggatgc accctgcggc acggtgcacg tcacctcaaa
     1081 actcgagaag tcatcgggct caagcccgcc gacaacgtag accgggtagg ggtcggccgc
     1141 ggccgccgga tcatcgacgg tgaccttgac ggcgtaggcg tctttgagcc cggcgttggt
     1201 aacgtcgccg ctcagggtga tcgtgctgcc gctcccggta acctcgatgt tgttcaccac
     1261 catatccggt tcgacaacac ggtcgccgat ctctatgggt atggtcaggg tggtgtggtg
     1321 ctcgttgatc ccgttccggt aggagagatc gaatgtgagt tcggtcgact gtgacgccac
     1381 gacactgaat gtggcggtct tctcctcatc aggtgcaaga gcgcccagga agacggccgt
     1441 ctcggttgtc ctgaccccct ccccggacgg agttacggta acaccgctca cactggttcc
     1501 gcgggggttc ccgaccgaga ggacgatggc gtcctcactg tttgccggga atgtctccgg
     1561 agcgtcgatc acgttgacct ggatcccggt gctctcaacg gtcaccggga tggagtagcg
     1621 gacgcttccg ccgtccctga ggtcaaggta gaagacagga tagtatatcc catcctcagc
     1681 atcggcccgc acggtaaacg taaacgagat ggtattcccc gggccaagtg tgcccacaga
     1741 gtcgtatgtc ctgtcgttta caacgacgac cccgctggag tagagttccg ccctgctgat
     1801 ggcgacgccg gtatccccgg tgttctgtat ctcgaccgtc accgtccccg tgtccccgcg
     1861 catgagggtt tcagggtcga gggtgacact ggtgaccgcg atctgttccg ccggtgtggt
     1921 ctcggccgcg ctggcggcgc caacgagcag tgcgaacagg agcaggacgc agaggttgcg
     1981 cctcatcggt cccgccccgg tatcattctg gttgtggtca tggctctttc actcgctaag
     2041 agtttctttg tttagttgcc gcccgttcag cctgcatgag ggagaaaaag gcgatcggtt
     2101 ttttgttaat aacattttac atattgttat atatatttta ctaaaagtca ggggggggta
     2161 aacagcgtgc cgcgaatcca gggcgcagag aaaacaggtg tgtcaattag gctcggggct
     2221 gcggttgaac cgccggcacc cgcgtcatgg gcgtgattgc ggccgattct ttatgagcgt
     2281 tcccggcaga ggggaaagga tctattgaaa ccccatgtgg gggtcttact cgcataacgt
     2341 cccgaaagga cggaggaggt gggtttgggg gcgggcagca ggcttgtggg tctgcccatg
     2401 gtggtatagc atgacaggcc gcttctttcc cccgcagggg tattggtcgt gctgccgccc
     2461 gacaggaatc ctcgtcgttg ccatcggaga gcggtgacgg gaatcttgtg tgccgccgcc
     2521 gtctggccgg tgttctgatg tggtggttgc cgccggtcag cgtttcagca cactcttttt
     2581 ccgggaacgc ctcattctgc ttttctttac cgtcccggaa aaggttgcgg cggagattac
     2641 gtatcgccgt attccatctg aaccgcgtca tcggtcagtc gctgtcgccg ccggtcatcc
     2701 cggagggcat ggtgccgccc cggtacggcc cggtctcttc ctggtcatcc acctccgttc
     2761 tgcttgggcg ggggtccggt gctcatggca gcggtctcac cccgccacgt atagtctatt
     2821 atacattttg ttattaaaat gttactcttc tcccccggtg taagggtccg ctgaaaacaa
     2881 gaagaccgcc tctgctcacc ggaaatccgg ataccggaac agggatttct acgcgaaatt
     2941 gcggcattta tgccggatca ggcaccctat accggttcgt cgtagatgaa gatctcctcg
     3001 atagaggccc cgaagtatcg cgcgatcttg aaggcaagtt cgagggaggg gtcgtatttc
     3061 cccttctcga tggcaaggat ggtctgccgg gtgactccca ggttgtttgc aagcgcttcc
     3121 tgggtcaggt catgcatcgc ccggtagacc ttaatcctgt tcttcatcgg tatcccattc
     3181 cccgtacttg cgggcgtagt agacccggaa tccgacgtat aaaaagatca tcaggcagag
     3241 cagggtcatc tggaagatgg caaaaccgcc gaaaaccggg agttcaagcg cttcggtggg
     3301 aaacacctcg tgcggctctg ggtgcggggg tctaagcccc agcggccttg agaacgcgat
     3361 gaccgcgctc ccgaggctga gaacaaaaaa gatcacccag gaggcctcca gagtccgcag
     3421 cgctgccttc tgcgtgatca ttgccgtccg ttcgtcctcg atcgtctctt cgaccctccg
     3481 ccgtgccagg taaagcagga taacgccgag gacgaaggca acctggatcg ggatcgggtt
     3541 ctcaagcctc accgagagcc agaatatgcc aacctccgcg agcgcaacga taccggccag
     3601 gaggtagaaa gtgtttcgtt tcatggtgca gccgctgtat atgatcttca acattttgtt
     3661 attaatgggt tacgccggcc ggccgtctaa aaaagaggcc atcaaaccaa cagccacact
     3721 ggtttgataa acctctccgt ggacagtcca cggacgatcc atggacagat tccggggggg
     3781 gtggccggga tctgtatgaa gatgctgccc ccatcttcat gcggtatgcc tctggtcaca
     3841 gcggcatcat gcggtttttt gcgagacggc attgaggaga gaggggagag gggaggtggg
     3901 gttggtcagg cgaaagacgt tcaaccagag acgcagcgac aggggagggg gatgttccac
     3961 ccccctcccc ggcgagccgc ctcccccagg gggcgattcc ccatagacgc actgccacgg
     4021 aagcagacaa cctctctgcc gtagacgcga tcaccctcac cgaaatccag ttcctgctcc
     4081 cccgtgcacg gctatccgga ctatcgccag acactgcccg cccccttgag ggggcgggag
     4141 gaggcgctgg aagcgccggg aggcgggggt ggtcgactgc tcgcatggaa aagacgtctt
     4201 tggtgggaca cagcgacagg ggagggggat gttccacccc ctccccgtca gcccctcccc
     4261 cacggggcga ttccccatcg acgcactgcc acgggagagc atcccctcac ccggtttcag
     4321 gcgtgaaact cctcgaagat cctgttttcc cccgtgcacc gtatccgggg ctatcgccat
     4381 gcactgcccg ccccctctcg cagggggcgg gaggaggcct tgatgagcgg ccgggcggcg
     4441 gggggtggtc tatgaatatc gttcaaccag agacgcagga gacaggtgag ggagtgggca
     4501 gtcgtaataa caggaaaaga tggggggttt atatcacaga accgttgatt tgacggcctc
     4561 aaaaaaaagt tctcagattg gagccccgcg tcaccggtgc gcaaaatacg cgttcaccct
     4621 gccgctcttc accgaatcta tcctggcaaa cccgatcctc tcgaactgga cgaccctccc
     4681 cacctcaccg actacaagag gctcgcagaa cccctccagg ttcccctcct gccggaggag
     4741 ggtgcagggc aggtttgcat caaccgggac ccactggatg atcggcgcct ttacctgccg
     4801 ggcctcatca agcgagtcgc cagcgtagga tacccgcagc gtatcacctt cccggtcgac
     4861 cctgacgtta tagaggtcct ttaacctgat cagaccctgc ccctcgatat ccctccgcgg
     4921 gagaagcacc ctgccatcgg ttacaagggt gcgcaccccc cttgcagggt cgttcgggtg
     4981 aaggagcgcg tgagcctcac ggcgcggcgc cccctcgatg ctgacctcga tcgggtcagg
     5041 cacaaagaag tagcggttcg ccttcgggtc gacgatctcc ctgtttctgg catacaggtt
     5101 ctcccaggag aacgatatat cggtctcccc catcccgata tcgatcatgg cgttccggac
     5161 ggcttccggc tcgatccccc ggcgcgcaat cgcccgcagc gtcccgagat gtatatcgtc
     5221 ccagcccgtg tatgtcccgt tccggatccc ctcgcgcatc cccgacgtcg agaggacaac
     5281 acccgagata cccatccggc cataatggta gtacatcggc ggtctccatc caaaataacc
     5341 gaagatgtag cgctgccgct gggtgtttgc gatatggtcc ttcccgcgga tcacatgggt
     5401 tatccccagg aggcggtcgt ccaccgcgac agagaagttc atcagaggaa ataccgttgc
     5461 ctccgttcgc gggtggagtg gagagttcac gatccgcatc gcggagtagt ctctcatcgc
     5521 cgggtcaggg tggccgaggt cggtcttcac cctgacggtt gcaccgccct cgtagaactc
     5581 tccatcaagc atcttctgcc agagttccag gttctcctca atcccctgtt tgcggcacgg
     5641 gcattccctc ccccggagtt tgagttcgcg gaaacgttca ggctcgcagg tgcagacgta
     5701 ggcgccgccg atctcgatga gcctcctgca ccagtcgtag tagacatcga gccgatcgct
     5761 ctggtaaacg atatcggtga tcccgagccc cagccactca acgtcctcaa gcaccatcgc
     5821 gtatgccgcc ggatcgaccc tcctcgggtc ggtgtcctcg atgcgcagga tgtagcgtcc
     5881 cccataacgc cggacgtagt agtcgtttaa gatggaagcg cgggcgtgcc ccagatgcag
     5941 cggcccgctc gggttcgggg caaaccgcat caccacacct ttctccgcat cctcgagcgg
     6001 tggaagttcc cgggtgtgct cgtgctcctc atcgagttcg gccaggagtt cgggcgcgag
     6061 ttcttcaagc ctcgccctgc gttccgatga actcatccct gccacctccg cggccacctt
     6121 tgaggcaatc gcccctatct cccgggcgct gctccggaac tccgggtgcc tccctagaag
     6181 cgtcccgatc accgtcccgg tctttggggg gctgtcgtgc ttcaccgcgt tctgcagcgc
     6241 gtagacaaag agcaggtgct caatatcggt ctccgtcatg gtatcagtta cctctgttga
     6301 caaagaaatc ggcgatctcg ccgagcaact gtttctcctc cgattccggg atgaccgaga
     6361 gcgcctcttt tgcaaccccc gcccgctcca gggcaaccgc ccggacctcc tcgatgacac
     6421 cggcatcccg gagcatggtg accagaccct cgatctcctc agcggagagc ggtctcctgt
     6481 atggggcaag gtcgatcccc ttctcgcgcg cccggatagc gatcagcgtc tgcttcccct
     6541 ccctgatgtc cgacgcccgg tccttcccgc tctcttcggt gtctgcaagg agatcgatca
     6601 ggtcgtcctg gatctggaac gcgataccgc tgtttatccc gaactggtag agtgcatccg
     6661 tctggacggg gtttgcaccc gcaagaagac cgccgattgc tgcggccgct ccataaaggg
     6721 cgccggtctt cttgctgacc atctccaggt actcaagttc tgagacgtcc tcccgcctct
     6781 caaactccat atccatgctc tgcccctcgc agatctcggc gcacgtccgg gcaagcatct
     6841 tcaccgcccg gatttttgca gcatctgccc cctccgcaag acagatgaac tcgaatgcct
     6901 ttgcgtagag cacatccccc gcaaggatcg ccgtcggttc atcccagagg gtatgcaccg
     6961 tcggaacacc gcgccgggcg gtgtccccgt ccatgatgtc gtcatggatg agggtgaagt
     7021 tgtgggtcag ctcgagcgag agcgccgcca ggatcaggtc atccgaggat cccttcctga
     7081 tagcgtccgc ggcgagaagg gtgaccgccg ggcgaagccg tttacctcct gcaagcagca
     7141 ggtgggcgct cgcccggaag aggccgccgt ggacgttacc gaagtagcgg tggagcatcc
     7201 ggtcgatcct gtcagcgttc atctccaggt attctttaag aatcatcatg cgcattcccc
     7261 taatttatac ggagattctg tccgttccgc aggacatgga ggtcgtttcc aagggtatac
     7321 ccgacctcct ccgcgaaatt tacatactcc cctgtcatgt cgatgtcgcc gtgtgacggg
     7381 atgatgtgct gcgggttgag gaggtggacg aactcatagt gatcctcacg gtaagcatgg
     7441 ccggagacgt gtagatcgtc aaagatccgg gcccctgcca tcctggcgcg ggcctcgatc
     7501 aggtaacgct gcccccggtt catcgggttc gggatcaccc tggccgagaa gaggatcttg
     7561 tctcccttct ccagcttgaa cggggtatcg cccatcacca tccgtgtcag gatggcgcct
     7621 gtctcgccct ggtgacccgt gacgatcggg acaaacttgt cctttccggt ctttatgatc
     7681 cgtcgaagcg tccggtcgac cgtccgccgg ttgccgaata tcgagaggga ctcagggaac
     7741 ccgaccagtt ttaactgttc agccgctgtg gcgtagcgct ccatcgaacg cccgaggaga
     7801 accggtttcc tcccgatctc gtgggcgcac tccgcaatgg tcttgatcct ggagatgtgt
     7861 gaggagaagg tcgagacgat tagcgcgctc ttgtcatcct cgtaactcgt gatggtatcc
     7921 cggacgaggt cccttgcgat cttctcgctc gggcagcgtc ctttatctgc gatgtttaca
     7981 cactccgtta cgagagcaag gacaccctcc ttcccgatct gccgcagcct ggcgaaatcc
     8041 ggtggttctc cgagcacggg tgtcctgtcc agtttgaagt cgtttgcata gattaccgca
     8101 ccctctggcg tatgcaggac cgggatgacc gtatcgatga tcgagtgctg gctccgcaca
     8161 aactcgatcg tcaggtgcgg ggatatggtg taacgctgtc ctgctttcag ggcaaacagt
     8221 ttgttgttca cgccgaactt ctgttcgccg gcgatctgct gccttatcag ttccgtggtg
     8281 tagggtgtgc tgattatcgg ggcgtcgtag cggtgtgcca gttttggtac cgcaccgatg
     8341 tggtccaggt gcccgtgcga acagacgatc gcctttacac tcccttcgac cgtgttcatg
     8401 atggtgtcat ccgggatggc cttcatctcg atgaggtcca gggaatgcat attctcgata
     8461 tcagcatcct catggatcat cacctggtcg aggcgcagcc ccatatcaaa gatgacaatc
     8521 tcttttccgc agcggacggc ggtcatattt ctaccgacct cgttaaaacc gccaactgtt
     8581 atgatctcaa tatccatgta gcctccttgc aggatctttt gttttgtcgt ttgattcaat
     8641 catctgcctg gtcaggccgg tgatatacgt cctggtgtgc cggagatccg ctaccgttcg
     8701 ggaccccgtc aggaacatcg ccacgcgaag gtggcggtgc atcccctcga tggttgcaga
     8761 tagggcttct tcactctcca tcgccggttt caggaggggc aatgccatac ccccgaggtc
     8821 cgccccgaga gcaagggatt ttgcaatatc gatccccgaa cgcaaccccc ctgtcgcgat
     8881 ggtcgggcct cctgctctcc gcacctcgca taggcttacc accgtcggta tcccccagga
     8941 gaggaacgcc tccccgagtt cagcgagttc aggggcggtt gcccgcaggc gttcgatctt
     9001 cgcccatgac gttcctccat acccgccgat atcgatcgcg cttgccccgg ccccaaagat
     9061 cttccttgcc gttcctatcg atataccgga acccgtctcc ttcacgatga ccggacggga
     9121 gaactcttca cagaggccgc gcaacgcctc caggcacccg acagcgctgt gatcgccttc
     9181 gggctggatc gcctcctgga gcaggttgac gtggatcgcg atcgcctgcg catcgatcat
     9241 ctcaaccgcc ctttccgccc actcgatgcc gtggtcgcgc agctggatga tcccgaggtt
     9301 ggcgcagagg aacgcgtggg gcgcctcctc ccgcacgacg gtgaaactcc cttcaagttc
     9361 cggattttcc agtgccgctc gctgagaacc gacacccata cccaggttat accgttcggc
     9421 ggcacgtgcc agccgccggt tcacctccag ggtgtccggg tgtcctccgg tcattgctgc
     9481 aatgaagagg ggggaatcga gttttgcgtc gagaaaccgt gtttttagat cgatcgcatc
     9541 catgtcgcac tcagggatgg cattatgaac aagccgcaca tccccgaatc ctgcatcacc
     9601 tgcctcaaca ggctgctcat agcatatctc caggtgatcg cgtttccgtg aggatgtggc
     9661 ggtatccctt ctcatttttt actcctttct ggttattgct gtcccgccgt gatctgttcc
     9721 gtccaggaac cggccgatct gcgagacatg gaagatctct gagtcgatac cggcatcggc
     9781 cagtatcagg agttcagcga tcttgccccg catgcccccg gtcacgtcgg tctttgccga
     9841 cccgcctaca tcgagcgact ccaggatctc ccggtcgatg cggggtataa cggatcctcc
     9901 tctgagcaca ccggcaacat ccgttgcaag cccaacgcgc ctgctcccga gggcgagggc
     9961 aagcctcgcc accacctggt cgccggatac gatgcacgaa ccccgccggg tgtccatcac
    10021 gacgtcgccg tgcagcacgg gcacgatacc gtgatccgtc atcccggcga tctggcgggt
    10081 ctcaaacgag accagacggc cgtcctctgc gagtgagagg cagaggggat ggatgccgat
    10141 cgcctcgatc cctgcctccc gcagggcctc gacgaccgcg gcgttcagcc gcgagaccgc
    10201 agcgtgggtg tggtagaccc cgggcaggtt atcccggccg agcccctcat cgacccggta
    10261 gcgccgggcc tccgggtggc cgcaggagcc cgcaccgtgg accaggacga gctcaatctc
    10321 ccggtgtgcg gcgagaactt ctgcaatctc gcgcagccgg gcgtgatcga tggtgcagcc
    10381 ccccgatttg tcggtgataa cgctcccgcc cagtttcagt accactctct cagtcattct
    10441 tctcttctct cgcaccgtcg gtgtctatgg ttgtgattat ggcccttccc tcactggcct
    10501 cgatcgcccc ggcaacccgg ttctttgcac gcctggggca gagggcgatg atcgatccgc
    10561 cgcctccagc cccggtgatc ttggctccgt aagcaccgct cgcccttgcc gccaggacaa
    10621 gccggctgct tgccgggtga cccacaccga gcgcctccag gagtgcatgg ttcatatcca
    10681 tgtactgccc gagttccttc gggttgctca tctcgtgaat ggcagcaagc gtcaactccc
    10741 cgattgcgtc cagtatcggg tttgcgattg cgggatggtg ctgccgcagg ttcgcgacct
    10801 gctccaccat cttagcggtg ctgtggggca ccatggtgtt tcccacaaca aggtggaggt
    10861 tctccggggg cagtctgcgt ttggactccc ccgtgatcat gaccatccca ccgtttgccg
    10921 agacgtaggt gtcggttggg ctggcccggc ccttctgcac ctttttctcg atggcaaacg
    10981 cagtctcggc gatatactcg cgtggatgcc cgagatcgaa ctcatcgttg atggcagaga
    11041 gcgtcgccac ggtcacagcg gccgatgacc cgagccctga cgaactctgg agttgcgagc
    11101 ggacgtagac gctgccgcgg acgcccatca ccttgaaaca ttcgctgatg tatggcgact
    11161 tagggcgcgt cgggttccgg gacttcctca ccgtgacgaa gacacggggc ttgatcgcca
    11221 tcgcgatcgc cggcttcccg tagaccaccg cgtgctcgcc aaacaggaag accttgcccg
    11281 gcgcgctcca ggttgccaaa ttacaccacc gctatcgctg cataccccac tacctgattg
    11341 taatcctccg ttacatcacc gctggtcgta taccgaagaa gcgcccctct ccttccaccg
    11401 agatgcctgc agacggtgca catggccgcg atcgggccgt agccgcagat ggtggcccgg
    11461 gtcgcgatga tccgccggta gaactccggt atgtcaaggg ttttgagcgc ctcgatcaca
    11521 tggaggtcct gccgccgcgc caccacctct ggaacgtaat gagagaagtc gcttgaggcg
    11581 acgatcctca catcccgccg ggtgcggccg atcgcgtcga tcaggtgctc cgcaagattc
    11641 atcgcagcct cgtaactctg gtctcccatg agaatgggcg cgatccttgc tctcgggaac
    11701 cggtacctga tgaagggcat ctgcacctca agtgaatgct cgctccggtg cgaggtctcg
    11761 tctatcctga gatcaagcgc gtcgacaaac tctttatcga cattgatgac cccgagaggc
    11821 gtctcccagg gcattgcgga ggcggaggtc atgtatccat gatgactggg acctataacg
    11881 acgaatgtac cgtcaaaatc aggttgtatg gtagagaaag cacaggctgc ggtctccccc
    11941 gagtagatgt aaccggcatg gggggagact atgccgtggg ctgagatatc tggatttttg
    12001 ttccggaaga atgcctccag aacctgctcc aggtggctgg gctcagcagg atagaacatc
    12061 cccgctacac tgcatcggcg catatccatc aagggtgagg tcttgtgctt tgatcttata
    12121 actctgtctc aaagtcctcg ggggtgagcg atgtggtcac gccgcgcagg cgcagcatct
    12181 cctttgtcag gagatagtat atcagcgaga gcgcctttct gcccttgttg ttcgtcggga
    12241 tgaccagatc gacgtacttc gtcatgttgt tggtatcgca gagtgcgaca acggggatcc
    12301 ctgtctggac agcttcatta actgcctggg cgtcgctgat cgggtctgtc accacaacca
    12361 cgtcgggctc gatgtacttg ttcaggcgct ggttggtaag catccccgga ataaaccggc
    12421 ctatgaccgc catgccgccg atggcatcgg caaacatctt tgccgggtac tgcccgtact
    12481 gcctggaggt gacgaccagg atctttgcag ggtcatactg cgaaaggaac tttgcagcag
    12541 tcttgatccg atcatcggtt gcctggatgt ccaggatgta gagtccatca ccgcggacac
    12601 ggtagatgaa cttcatcatg tccttgctct tctgctgggt gccgatgtgc acgcctgccg
    12661 ccagataatc ttcgacggac accagcggtt ctttaagttc gatctcaagt tcgtttccgg
    12721 tcaagttaga tcagctcctc tattcgtatc agttcgttca gtttggctat ccgttccccg
    12781 ccgaccacgc cggtcttgag gaagatgcat ccgaacgccg ttccaaggtg cgctatggtc
    12841 gcgtcggttg tctctccgga acggtggctc atgaccgtct ccaggccgtt ctcatcggcg
    12901 aggcgtaccg cctcaaaggt atcggtgagt gttccgatct ggtttggttt gatcaggacg
    12961 cagtttgccg cgcccgtttc gatcccctgt atgatccgct cgacgttggt cacgaagagg
    13021 tcgtcaccgc agaccaggca ccggtctccg acctcctcgg tcaggctggc gaaggcctcg
    13081 aagtcctcct caaagaacgg gtcctccacg tagacgaggt tgtagcggtc gacgagatcc
    13141 gccgcgtagg cgatctggtc atcacggttc cgcacagcat ccctgtagcg gtagtgctca
    13201 ccgtcccaga gttcggttgc tgccatgtcg atacccatac ggatctcgac gttcatctca
    13261 tccgagatgg ctccgatcgc ctctcccagg atctcaaacg cctccgcatc ggttatcgcc
    13321 ggcgcccagg cgccttcatc ccctttcccg gagagtttgc cctgcgcctg caggatcttc
    13381 ttcacggcct tatggacggc agcgttcaca aacacggcct ctgtcgcccc ggaagccccg
    13441 gtggcgacca ccaggaactc ctggatggac gttgcgttcg gtgcatgagc gcctccgcct
    13501 atgatgttgc caagcggcag cggcgtctcg gaagcgaacg cacctccaag gtagcggaag
    13561 acctccaggt caagcgacga cgcagccgcc tttgcacatg caagcgagag tgcgacggcg
    13621 acgtttgcgc cgatcgagct gaaatagggt gttccatcga tctcccggag cagtgcatca
    13681 aacgtgatct gatcacatgc gtcctccccg atgagcgatg gaatcaggtg ttcccgcgcg
    13741 ttctcgatgg cttcgcgcgg cggccgcacc tttgcttcat aggttcctgt gctggcgccg
    13801 ctgggtgccg cggcccggcc gaaaccgcgg gatgtgtata tatcagcctc aacggtctcg
    13861 tttccgcggc tgtccaggat tgttctcagg gtaatctctt cgatggtcgt catcaggtca
    13921 caacttcctc ttcactgtta tggggatcac gtctcgctcg aactcttcga gtgcaatctc
    13981 aagcggctca gtctttctgg tctttaccag aacgggtgca cccatcgaga tctgaagagc
    14041 acgagcccct ataattcgag cccgttcata ccgggtatat gattccatcg tgcagcctca
    14101 aaatcattaa ttgcaaaatg gggtcgctga gatttgaact caggtcacag catcccgaac
    14161 gccgtaggat ggccaggcta ccccacgacc cctcattgat acgggttcag gtcctccacc
    14221 gcttctttgt gcgtcagcag catccgcctg cagcagtaac gttccaggcc gagatcgtcg
    14281 aggatctgtt tgggatcctc acccgcatct cgccgctcct tgaactcttt ccaggcggtg
    14341 gaaatgacct taccgcatgt aaaacatcgt acgggtatca tacctgttgt ccagtatccc
    14401 ttatatctct tttcctcagc gataagactt ctggaacttc gcccgtgcgc caggaccctg
    14461 cggtttcttg gtctctttct gccgcgagtc gttcacgagc agtgtccggt catacgacag
    14521 gaacgcatcc tttatctgcg ggtcgttatg ccactccagg atgccgcgcg ccagcgctgt
    14581 ccggacggcc tcggcctgcc ccattctgcc gccgccggag acctcgatcg cgacatcaac
    14641 gccgtcgagt gcgtctggaa ccagcagcag cggttcagcg atcttcatcc ggatcacctc
    14701 ggtcccgtag atctccaggg gcacagagtt gatccggata cggccgttgc cgggcttcag
    14761 ggttgcccgg gcgattgctg tcttcctctt accgcttgaa ttgacgatct ttaccatcca
    14821 atcaccatgt tttagtattt cgctccgagt ttggtgctta ttgtcccgat cgttacgtat
    14881 ctcgggctgc tcagccggtt gatatgcgcc gcctcaagcg tctccatatc catcccgctg
    14941 aactccgccg gaatcccaac gtagaccctg acccgcccga gtgcggctgc gccacgctca
    15001 cgcctgtagg gcagcatccc gcggatggtg cgcttgacga tctggtccgg cctgcgcgga
    15061 aagaacggtc ctccctcacg cgacccacgc tcgcgtttca tggtgtagtt tgcgagcacg
    15121 tgggccttgc tccctgagac gattgccttt tccgcgttca cgattgcgat ctcctcaccg
    15181 gcaagcgcac gctgtgcgac gatgctggcc atccgcccga gcagtaatcc atctgcgtcg
    15241 ataacctgaa ccatttgctt cacctgagga tccgtaccct gctccctttc gggttgtctc
    15301 gaataaggtc ttcgatggtc atgcacgtgc ccctggcgct ggtgatcttg gtcaccgcag
    15361 tctcggagaa gtcaagtgct gcgatcttca ccggcaggct gagcgcaccg ctgccgagaa
    15421 cctttcccgg cacaaggatt atctcatctt tgtttgcgta gcggttgatc ttgctgaggt
    15481 tcacctcggc atagtttttc cggggtgcat ccagcctctt tgcaatctcg cgccagatgt
    15541 tcgcttcatt tacacgcgac gcctctttca gcgtcgcgat gagagcggtc agccgggggt
    15601 ttgatttgtt tgcggttgtc tttgtcatta catcccctct cccgttatct cgttcataac
    15661 gtctaccagg tcatctgatt gtttctggat gtattgtagc gctctctcga tgatctcacc
    15721 gaccggcatc gaaccatcgc tttcaaccac gaagatgaac ttctccgggt cggtgccgat
    15781 atggatggca ggttcatcgc cgattccacc ggcaaggcat gcccgctcac agagcctgca
    15841 gagggaacat gactccagtc gcccattgac gaccctgaca cgcccctctg ctgcttccag
    15901 cacgctgcgg gggcactcct caaggcacat gccgcagccg tcgcaccgct tgtcgatggt
    15961 tattgccgga tagttcttgt agccgcaggc ggttgtagcc tgccacttgg catgctcctt
    16021 tccggtcccg agtatggcct gggcttcgag cacgaccttc tggtcatttg aaagttcgat
    16081 gatcgggata ttctcctcgg ccggtgcggc atccgggtcc tgcgggatca ggtcgcttga
    16141 gtagacggtc tttggcccct cggcggatag cgtgtaggtg gccgtgcaga actgacatcc
    16201 tgcacctccg caggtgcatt cgctgcgcag cctgtattgt tccagatccg tccgcagggg
    16261 gatgagcccc aggcggtgtg ccagcatctc gtcgaagagg acgcttgtat tgtcgtagat
    16321 gcggacattc tcaatggcaa gtgtcggcac ctcgctcatc atcgcccgcc ggaatatgtt
    16381 ggcaaacgac gtggaaacgc cgctcagggt gaactttgcg actctctcgt ccagtcgaga
    16441 aaacgctatc tccattacac tctccttcct ctccggccgc ctttgctgcg gatgctgtcg
    16501 tgcggcaccg gtgtgacgtc ctcgatgcgg ccgatcttca tccctgcacg ggcgagggcg
    16561 cggatggctg cctgggcgcc tggcccgggg ctccgctgtt tgcccagtcc cggcgcccga
    16621 acacggacgt ggagaccggt gattcccttc tcgcgtgcgt tctgtgcgac ctgtgttgcc
    16681 atctgcatgg cagcgtaggg cgaactctcg tttcggtcct gtttcacgac catgccaccg
    16741 ctgctcttcg tgatggtctc cgcccccgag aggtcggtta tggtgatgat ggtgttgtta
    16801 aacgaggcgt agatgtgtgc aatgccccat ttctcttctg ccatggtgtt acctcactcc
    16861 tgcgcgggtg atgcgccctc tctccgggtg gacttcgttt gtgagcgggg agacctcgta
    16921 gtagccgatc tctggctcct cttccctggt gacccggtag cccggcaccg tcacgcgctg
    16981 gccgccgatc gcgatgtgcc cgtgggtgat gaactgtcgg gcctgttttg gggtgcgtgc
    17041 aagccccttg cgaagcacct gcgtctggag tcggcggtcg agctgctgct ctaccttgag
    17101 ggcgagaacg tcgcccacat cggcattctc tccgacgaga ccgtagcggt agaggtggct
    17161 gatgagctgg tctttcttcg tctggtagat ctcggaggtg atgcccgccg acctgagagc
    17221 caccagttca cgggcggcgg cacgatactt ccgcagaatg ctctgggctt tccagagttc
    17281 gcgcttgttt cgcagtccgt aatcgatgac aagccgcttc tcgtcatcaa gccgcgtctt
    17341 ctcgaaccga cgcttgggtg ttgcatactg cttgtggttt tttcctggat atgccattca
    17401 atcaccgctc atattttctt tctccggaca ccgacggtca tgccggttct tccggtggac
    17461 tttgtgcgct ggccgcggac cttctgcccc gtctcgtgcc tgatgccacg gtaacaccgc
    17521 atctttctca tgcggttgat gtcgtcttca atcgccaggc tgagatccgt tccgaggagg
    17581 tggcgtttct ctccggtata gacgtctttt gggcggttga ccatccattc gggaacctga
    17641 gtggtgtagg tctccacagc gtctgagatc cgcctgatcg attcgtcatc gagtttaccg
    17701 aggacggcgt gcgggtcaac accggcaagg gcggtgatgg tgcgggatgt gtggtgaccg
    17761 attccgttga tccctgtcag tgcgatctgc accgttttcg tgccgtcgag atcagtgttc
    17821 ctgatccgga caaagtactt tatctcttca tcatccatgt gatcgcctcg tcatcgacat
    17881 cgtcggtgaa agcgctgagg gagggatttg aaccctcgag tcccagaggg acacaggttt
    17941 agcaaacctg cgccatacca ggcttggcta cctcaacata gaatctcgca tctttcgaca
    18001 ctcgcaacac cgaaggtgct gctccagaca cagcattcta tggcttatgc ctagttatgt
    18061 ttgcagggtt ggctaataac tattgtggtg gggtgacccc tgcggtcaac tccgtgaatc
    18121 ttcttcccgt ttacgcggga tcgggttgtg gtcggcgttt catacccctc tctgatccgg
    18181 gggttgggtt cggccggtcg ccggcacgat ggaggactcc ggcggtgcac ggtttacggc
    18241 gcgcggcggg cccgtttgac cccgctcccg atcaggcccg aggcaacgat gggaagcgct
    18301 atcgttgcgt cgcagaagca ctggacgcgg tgcgactccg gcgcctcttt gccccagctg
    18361 atcgcctcct cgaatgtgca gccggagagc ccgccccagt ggggtgcgtc ggtggtgtac
    18421 tggatggcat atgcgtggcc gccgaggttc tgttcgtgga tcgaggcgat gacctgggtc
    18481 tgctggatga agttcttcgg gacgccgccg ccgatataga tcacaccggt ctttcttgcg
    18541 ccctcgacca tgcgggtgat ctcatcggtg tcggcgagtt gatcgatgtc tatgttgaca
    18601 ccgcgtcgac gtgccatcac cagcccgatc ccgatggacg agtcgcagag tgccgggatg
    18661 aatatcggga cgccgcattc tgcagaggtg gcgacgagcg actgcccttc aggccggttt
    18721 tcgcggagcc agccccccag acggcgggtg aactcgcgtg atgagccctg gaacggggca
    18781 atgctcccgg cgaacctggc aatctcggcg tcgacgctcc ggaactcctc ttcgtaggcg
    18841 aagacatcgt atatgcggtc gatcccttcc tcaaatagcg ccgcgtcgtc cacatgttga
    18901 tggccgatgt agtgccggac gcccaggtgt tcgcaggtgt cgtggaagat gtttgcaccc
    18961 gtcgagacta tgacgtcgac gtagcgatgc ctgacaaggt cgatgagcac gttctgcata
    19021 cctgccggga tcatcgcacc agagaggccc atgaatatag tgcagtctgg atcggtgatc
    19081 atccttgtcc agacgccgag cgactccccg agttttctac cctggaagcc ggtcatgctc
    19141 atggactcaa ggagggccgt ta
//
LOCUS       DAOSZX010000020        18508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2484_length_18508_cov_22.631009, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000020 DAOSZX010000000
VERSION     DAOSZX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18508
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2484_length_18508_cov_22.631009"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..912)
                     /locus_tag="PK196_03110"
     CDS             complement(70..912)
                     /locus_tag="PK196_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HON40443.1"
                     /translation="MVVKVGIAKLGNIASGVMAELLLDERADREDIITFMATSGTKLQ
                     PEDIDRVVTNMKAWGPDFCIVVSPNGVLPGPTKARENLAAAGIPCIVITDDVTTKKEQ
                     FEALKASNFGYIIMKADAMIGARREFLDPIEMADYNGNLIKVLAITGAFRKMQMELDK
                     VIDQVKAGKKGADLVLPKVVMTSDKAVEGEFSNPYALAKARAAYEIAQEVAGVNVKGC
                     FMTKEWEKYIPIVASAHEMMRQAMLLCDEARNLEKAVDGVIRKPHKKTGEIVSKTALI
                     SKPE"
     gene            1049..1453
                     /locus_tag="PK196_03115"
     CDS             1049..1453
                     /locus_tag="PK196_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HON40444.1"
                     /translation="MKQGLLTDRQKEVLRYRKQGLTQQQIAEIIHTSKANVCTIEKAA
                     LENISRARETLEFLYTLDAIHLCTLHKGLDLLKAPEIIYAEAEKQGVKVKYDTITLIN
                     RLRDANPERFKARQVREDVEVYINQDGDLYFG"
     gene            2225..3238
                     /locus_tag="PK196_03120"
     CDS             2225..3238
                     /locus_tag="PK196_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HON40445.1"
                     /translation="MPKINRPRRGSLAYSPRKRAKSPVPRYGSWPDYTGTPAVQGFAG
                     YKVGMTHVIMVDDHKSSPTEGRDVMVPVTVIEVPPMRVAGVRAYTEDTYGKRALTEAW
                     ATDLDREVSRRINLPKNHDTAAALGAIKTAISEGRVVELYALTYTRPAEITGVPKKVP
                     DIMEMRIAGGSLEDQIAYAADILGGEIAISDHFEAGEYVDVTAVTTGKGTEGPVRRWG
                     VQVRKRKHSRGGKKRHIGNLGPWNPHHVRWQVPQMGQMGYQQRTEFNKRILKIGADSN
                     DITPAGGFLQYGVVRGPYVLIKGSVPGPSKRLVRIRSAIRQGEHVVRTPTIGHISVQS
                     QQG"
     gene            3245..4000
                     /gene="rpl4p"
                     /locus_tag="PK196_03125"
     CDS             3245..4000
                     /gene="rpl4p"
                     /locus_tag="PK196_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HON40446.1"
                     /translation="MKAQVRTLTGEIAHEIDLPAVFGEDYRPDLIKRAVIALQSTRFQ
                     PHGTNPYAGIRTSAESWGSGRGVAQIPRIKNGSRAARVPQATGGRAAHPPKVEKVLVR
                     QINRKEKRKALRSAIAASTHPDLVQARGHLFEGSLPLVMEDRFEGLTRTSDVIAALSA
                     VGIYADVERAKGSRKVRAGRGTMRGRRYKQRKSVLIVTGTEPLRAARNLAGVDAVAVN
                     QLNTELLAPGTQAGRLTVWTESAIRRLEEFSWY"
     gene            3991..4239
                     /locus_tag="PK196_03130"
     CDS             3991..4239
                     /locus_tag="PK196_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HON40447.1"
                     /translation="MVLKHPFVTEKAMLMVEGGNELQFIVERDASKQEIRRALESAFG
                     QEVRSVRTMMTTKGQKKAIVSFADAKAAEEILSRLGIM"
     gene            4251..4970
                     /locus_tag="PK196_03135"
     CDS             4251..4970
                     /locus_tag="PK196_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HON40448.1"
                     /translation="MAHRIIAQNRGKGGPTYRAPSHRYKAPLRHAGKNDSLIKGLIVD
                     IEHDPARHAPIALARLEDGEKVYMLATEGLGVGDTVSWGSGGAVKNGNTLPLREIPVG
                     AYVCNIEARPDDGGKFVRSSGVQALVIGKADDGRVGVRMPSGKNKWFNGGCMATVGIV
                     AGGGRGEKPFVKAGKKYFHVRSSAEKWPRVKGVCMNVIDHPFGGGGHQHCGRPKTVSR
                     GTPPGRKVGHIAARRTGKWKK"
     gene            4981..5394
                     /locus_tag="PK196_03140"
     CDS             4981..5394
                     /locus_tag="PK196_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HON40449.1"
                     /translation="MAKKTQKRMPRRREEFTYRGHSIADLQQMALSELLPLMPARARR
                     KFDRGLSREQEKLLADIRSGEAPLRTHLRDMVIMPEMVGKTVEIHNGKEFQKVEIQPE
                     AVFHYLGEFALTRKKVSHGSAGIGATRSSKYVPLK"
     gene            5400..5861
                     /locus_tag="PK196_03145"
     CDS             5400..5861
                     /locus_tag="PK196_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HON40450.1"
                     /translation="MARTGYSTEIEGENVARAKANEIPVSPKHSIEIARFIRNMTTSE
                     AKAYLADVVALKKPIPFKRFKRNVAHKRGLSKWPAGRYPVKAAEAYIRLIESVEKNAE
                     YIGLDSTNLRIEHVAANRGRGFRSFFPRAMGRATPKRRETVNIEIIVTEVA"
     gene            5861..6553
                     /locus_tag="PK196_03150"
     CDS             5861..6553
                     /locus_tag="PK196_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HON40451.1"
                     /translation="MAIEKRFIADGVRNVRVEKFLMKELKRAGYGGMDITRTPLGTQV
                     TIFAEKPGIVIGKGGKQVRQLTQDLASVYGIESPQVEVQQVQNPTFNAQIMAERLANA
                     LERGWYFRKAGQSTIRRVMDSGALGCEVVVAGKLTGARSRTQKFIEGYVKHCGEPSET
                     IVEKGYAIAIKKLGVIGVQVKIVPPDAKLPDTFEIIEPEPKEIPAQPPVVENADEDEF
                     EEMPDDDYEEEI"
     gene            6554..6760
                     /gene="rpmC"
                     /locus_tag="PK196_03155"
     CDS             6554..6760
                     /gene="rpmC"
                     /locus_tag="PK196_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HON40452.1"
                     /translation="MAIFRAHEVRQFSDIELLEQEQKLSLELIRERGKVGAGGATEDP
                     GRIREIRRTIARIKTEQNARRRNA"
     gene            6757..7029
                     /locus_tag="PK196_03160"
     CDS             6757..7029
                     /locus_tag="PK196_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HON40453.1"
                     /translation="MISPRNILRHELIGLDVLVVRASNPAHVGLSGRVVGETRNTLLI
                     QTGRGEKRIPKRLSVFRLRLPDGATIDIDGSSIEMQPERRISMRIK"
     gene            7043..7369
                     /locus_tag="PK196_03165"
     CDS             7043..7369
                     /locus_tag="PK196_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HON40454.1"
                     /translation="MARNIGLGVPIPEKECEDANCPFHGTLPVRGQVITGKVVSDRMN
                     ATVVVEREYLHYVKKYNRYEKRRSRYHAHNTPCLNARVGDQVRIAECRPLSKTKNFVV
                     VEVMKK"
     gene            7366..7764
                     /gene="rpl14p"
                     /locus_tag="PK196_03170"
     CDS             7366..7764
                     /gene="rpl14p"
                     /locus_tag="PK196_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HON40455.1"
                     /translation="MKAMQAKIPRALATGSRMVCSDNTGARVVEVISVDGYHGVRRRQ
                     PCLGIGDIATVSVKKGTPDMRRKLEKAVVIRQKKEIRRPNGIRLSFEDNAMVLINERG
                     EPKGTEIKGPVPREIAERFPKIASMATMIV"
     gene            7777..8142
                     /gene="rplX"
                     /locus_tag="PK196_03175"
     CDS             7777..8142
                     /gene="rplX"
                     /locus_tag="PK196_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HON40456.1"
                     /translation="MVRITSKQPRKQRKARYNAPNHTRGRFLTASLSPELREKYSTRR
                     VRVVSGDTVKVFRGDFAGEEGIVDAVDMKACRLVVHGVMVTKADGTEVPRPVDPSNVQ
                     ITKLNLKDRLREERLGGGA"
     gene            8142..8873
                     /locus_tag="PK196_03180"
     CDS             8142..8873
                     /locus_tag="PK196_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HON40457.1"
                     /translation="MSGHLKRLAAPGSWRVPAKTKKFVMKTAPGPHNADALPVGVWLR
                     EHLGLAQNASEVRKILHQQDVIVNGRACKNPQMGLGVFDIVSIPKIGKHYRIQLDKRG
                     NYVAVGIPAESAKTRLCRVRGKTTLSGGRVQLNLGFGANILADNTYRATDSIVVTLGI
                     DGDERFQIVDHFPFAEGNVAMIIGGKHSGKVGRIVEIIRTASPLPNRIVLVDDATDER
                     FETIEDYIFMVGRSAIAPELEVVSS"
     gene            8870..9376
                     /locus_tag="PK196_03185"
     CDS             8870..9376
                     /locus_tag="PK196_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HON40458.1"
                     /translation="MSNPMQEIYIDKVVVHMGVGESGERLVKAEKIMQQITGQKPVRT
                     IAKRTQPAFGIRKGAPIGCKVTLRRENAEKFITTALDIIDRRLAPSQFDRTGNVSFGI
                     EEHTDFPGMSYDPMVGIYGMDINVVLEHKGVRVARRRIERRKLPETQKVSRDDALAFM
                     RERYLVEV"
     gene            9380..9559
                     /locus_tag="PK196_03190"
     CDS             9380..9559
                     /locus_tag="PK196_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HON40459.1"
                     /translation="MAEESGAPASGKGVKKFGRGANECRICGRKQGLVRKYDIYFCRQ
                     CFRENARKMGFKKLN"
     gene            9571..9963
                     /locus_tag="PK196_03195"
     CDS             9571..9963
                     /locus_tag="PK196_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HON40460.1"
                     /translation="MARLNPIADAMCAIKNASDAGKSEVIVEPASKLLGAMLRVMQEN
                     GFINGFEFIDDGRGGQFRVQISGTINKCGAINPRFPVAMAEMEYWESQYLPAKNFGIL
                     IISTSKGVISHTQARNEGIGGELLGYVY"
     gene            9978..10502
                     /locus_tag="PK196_03200"
     CDS             9978..10502
                     /locus_tag="PK196_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HON40461.1"
                     /translation="MGITRKVRIPSGVNITLEGGVLTVSGPKGRLVRDMRFPQIDLGI
                     EDGFIVISTASDKKRFLAMSGTLEAHAENMIRGVVEGYEYRMKVVYSHFPIQLKLKGD
                     RLEINNFLGEKQPRLAKILDGVTVNLGNDEVVLSGIDKEKVGNTAANIEHATRIRKRD
                     PRVFQDGIYITERA"
     gene            10508..10939
                     /locus_tag="PK196_03205"
     CDS             10508..10939
                     /locus_tag="PK196_03205"
                     /inference="COORDINATES: protein motif:HMM:NF006332.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HON40462.1"
                     /translation="MDEKRRLIRVRTSHNKPAFKRRGLHRKVKLADVWRRPRGLHNKQ
                     RRQFRAKGALPGPGYGGPTAVRGMHPSGYEEVRVFSPADLDGLNPETQAIRIAGSVGN
                     RKRGVIQERAMEMGLKVLNAKDLTRVAGEAVAAEEEVSEDE"
     gene            10932..11384
                     /locus_tag="PK196_03210"
     CDS             10932..11384
                     /locus_tag="PK196_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HON40463.1"
                     /translation="MSDLGNQRRMAAAILKCGVNRVWFDPERQADIEAAISRKDLRDL
                     IGEGAIRANPVKGNSRGRTRARMAKRSYGHRKGSGRRKGAAGSRNPGKRAWIRKIRAQ
                     RRLLREMRDEGTIDRSVYRLMYRRASGGQFRSVAHLKSHVDMMAGGME"
     gene            11384..11911
                     /locus_tag="PK196_03215"
     CDS             11384..11911
                     /locus_tag="PK196_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HON40464.1"
                     /translation="MATGPRYFVPFRRRHEGKTDYYKRMALLSSGMPRMVVRKTNRQI
                     IVQLIVPGVEGDRTLVSAYSTELAGYGYEGSGANTPAAYLTGMLFGVRALNAGYSEAI
                     LDIGLARAKPGARVFAALRGAVDAGLNVPYGESILPAEERLRGAHIAEHDPDRAGNLV
                     ENVEAVALAIKKELV"
     gene            11914..12531
                     /locus_tag="PK196_03220"
     CDS             11914..12531
                     /locus_tag="PK196_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HON40465.1"
                     /translation="MAYVEEEWIPLTGLGRRVAAGELTSIDEVLASGRPIKEPQIVDV
                     LLPDLEDEVLDINMVQRMTDSGRRVKFRTTVVVGNRNGYVGFGQGKDVQVGNAIQKAI
                     ADAKLNLIKVSRGCGSWECGCDAAHSIPFEVTGKAGSVRVTLKPAPQGIGLVTGDIPK
                     KVLALAGIKDVWAFNRGETRTTINYAKATFEALRKTNMVRIGGTE"
     gene            12531..12992
                     /locus_tag="PK196_03225"
     CDS             12531..12992
                     /locus_tag="PK196_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HON40466.1"
                     /translation="MYAVVQVRGVVKTNREIRDTLKMLRLHHVNHCVLVPETPAYQGM
                     IRKVKDFVAYGEVDEETLATLLRTRGRLTGDERLTDDYIREHTPYADIDEFAAALCSG
                     ETSFADLVEIKPVLRLHPPRKGFKTIKRTFQQGGALGYYGPRINDLLYRMR"
     gene            12998..13423
                     /locus_tag="PK196_03230"
     CDS             12998..13423
                     /locus_tag="PK196_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HON40467.1"
                     /translation="MPVNKRSKYRGSRTCGGGTHKNRRGAGNRGGRGRAGQRDHRFSH
                     FYLRGEISNGKHGFVNKTSVPVSAIDVGDIDQMIEALVSEGFATREGDLITLDATEIG
                     IEKVLGGGQVTHRMSISARAFSERARAKIEEMGGQAMTV"
     gene            13444..14877
                     /gene="secY"
                     /locus_tag="PK196_03235"
     CDS             13444..14877
                     /gene="secY"
                     /locus_tag="PK196_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HON40468.1"
                     /translation="MGDLLDRFEPILAAMPAVRNPEGHVHFKNKLMWTVAILLLYFTL
                     TNIDIFGLSPQSQDLFGMYRALLAGASGSLLHLGIGPIVTASIVLQLLKGADLLQIDT
                     SEARGQVMYMGLQKMLIFVMIVIEGLPMIVGGMMLPDPAVAAEFFGGNTFIVSVLIFL
                     QICLGGLLVVFMDEVVTKWGIGSGVGLFIVAGVSQGLVNGFLNWQTGTDPFPIGFFPR
                     LFAVATSGVNFLEYFGTDLLALVTTIAIFLVIVYVESTRIEIPLAHTAVRGARARFPV
                     KLIYASVLPMILVRVLQANVQMIGMLLSNAGITILGEFQGQVPVNGLMWYIAPINQPQ
                     DWMWWIADLGHAPWEILLRMGIDVGVMVIGGAIFALFWVKTAGLDSKAVARQIQRSGM
                     QIPGYRRNVQVLEKYLDRYIPRITVIGGVFIGLLSVVANLFGVIGAVGGTGLLLAVSI
                     TYRLYEEIASQQIMEMYPFMRGFFGKE"
     gene            15096..15671
                     /locus_tag="PK196_03240"
     CDS             15096..15671
                     /locus_tag="PK196_03240"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HON40469.1"
                     /translation="MGKKVVVTGVPGVGKTTVINGAMERLAAEGIAYEAVNFGTFMFE
                     AARRENLVTDRDEMRKLGKDVQKRLQKEAAAGIAAMSVDASVIIDTHSSVKTPAGFLA
                     GLPEWVLHELMPDIIVLVETDPDQILMRRLGDPSRSRDMEGSRAIAEHQEFNRAVAAA
                     YAMYAGCTIKIVRNEDFLLEHGIDDLVSVLR"
     gene            15678..16304
                     /locus_tag="PK196_03245"
     CDS             15678..16304
                     /locus_tag="PK196_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HON40470.1"
                     /translation="MVDLKKQAPTIALLFTLLVFLSYSVSWVRLTIGTAMDVVLGPLI
                     DPEGFAVPFFVMIIILSSLTGLYSSLVQKYTIDYEKMQETQAKMRVFQKEFREAQLSG
                     DEKRIKKLQKKQEQMMQDQLEISRQQFVPMAIILLLTVPIFFWILLRLPAVGAAADIA
                     TGIVMPFAGIVSLSATVLWFIPAWIIWYMLCSLAMSQVIRKSLNIGGI"
     gene            16304..16852
                     /locus_tag="PK196_03250"
     CDS             16304..16852
                     /locus_tag="PK196_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON40471.1"
                     /translation="MRITISGPPGSGTTSLARYLAGKHGFDLISAGEVFRQLAREHGM
                     DLAEFGKFAESDPSADRMIDARQKEIGEAADDIIIEGRLSGWMIVNADLRIWLKASPE
                     CRAKRIAERDGMDEEAARVYTENRQRSEATRYRNYYGIEIDDLSPYDIVLSSETFGVE
                     ALGAIVDTAIECLRRQRGDVGQ"
     assembly_gap    16927..17026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17335..18162)
                     /locus_tag="PK196_03255"
     CDS             complement(17335..18162)
                     /locus_tag="PK196_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HON40472.1"
                     /translation="MDHRHPVVLVHGWRSHPGIWNHLAPALQDAGLPCWKFDHTAIQE
                     NGTEAIALALQEFIRARRKENGYGGPVDIVCHSMGTCIARYLLEVIDGSDREEDVRFL
                     VGIGPPNKGSAMAEAFNDPEQGPEVIRRLAGVFVPRGYDPTEDLIVQEFRPGSRVTSA
                     LSRAGTRDDIAYRLILAANLTRTPAFFPAFGGRTLEFAPGEGWRMTYAGDGIVPHSDS
                     HLPGAGVEILPADPANLGRHPERYCHIMLPRNPEVIAHVRSCLAASAAPDVRTPPRS"
BASE COUNT         4080 a         4885 c         5762 g         3681 t
ORIGIN      
        1 aaaagagtca gcaccctcct gacgattggc cggtgtcagg gtcggccgga aaaaaaagtg
       61 gtgttggggt tactcgggct tgctgatgag agcagtcttt gagacgatct cgccggtctt
      121 cttgtgcggt ttgcggatga cgccgtccac agccttctcc aggttgcgtg cctcatcgca
      181 gaggagcatg gcctggcgca tcatctcgtg ggcgcttgca acgatcggga tgtacttctc
      241 ccactccttc gtcatgaagc agcccttgac gtttacgcca gcaacttcct gagcgatctc
      301 gtaggcggca cgggccttcg cgagcgcata ggggttggag aactcgccct cgaccgcctt
      361 gtcggaggtc atgacaacct tcggaagcac caggtctgcg cccttcttgc cagccttaac
      421 ctggtcaatg accttgtcga gttccatctg catcttgcgg aacgcaccgg tgatggcgag
      481 caccttgatc aggttcccgt tgtagtctgc catctctatg gggtccagga actcgcggcg
      541 ggcgccgatc atggcatcgg ctttcattat gatgtaaccg aagttgcttg ccttgagcgc
      601 ttcaaactgc tccttcttgg tggttacatc gtcggtgatg acgatacagg ggattccggc
      661 ggcagccagg ttctcacgtg cctttgtggg tccggggagg acaccgttcg gggagactac
      721 aatacagaag tccgggcccc atgctttcat gttggtaacc acacggtcga tatcctcggg
      781 ctgcagtttg gtgcctgagg ttgccatgaa agttataatg tcttcacggt cggcacgctc
      841 atcgagcagg agttcagcca tcacaccact ggcgatattt cccagttttg caattccaac
      901 ttttacaacc attttaacac ctctcaatat ttgcgaacac gataatattg ccaagttctc
      961 atataaacgt tttgaaatgc gccgatcacc tcgctgtgat ggtgggttaa ggaaaagtgt
     1021 ttaaattttg atccctcata catatttgat gaagcaaggt ctgctcaccg atcgtcagaa
     1081 agaagtgctg cgctaccgca aacaggggtt gacgcagcag cagatagccg agatcattca
     1141 cacctcaaag gcaaacgttt gtactatcga gaaggctgct ctcgagaaca tctcacgtgc
     1201 gcgtgagacg cttgagttcc tctacaccct tgatgccata catctctgca cactccacaa
     1261 agggcttgac ctgctcaagg cgcccgagat catctatgca gaggccgaga agcagggtgt
     1321 gaaggttaaa tatgacacga tcacgctcat aaacaggcta agggatgcaa acccggagcg
     1381 gttcaaggca cgacaggtca gggaggacgt tgaggtgtat atcaatcagg acggcgatct
     1441 ttacttcggt tgatcatcgg acgtgtggag aaggatggta tcctgcctga gcgagagcat
     1501 tttgtggcac ctggaggtcc tcatctaccg cgctggctca cggtgggcct gctaccccct
     1561 caaaacgatc gcaatccgac aatcaggtgc aggccctgcg gaaaagcggt gtatcagaag
     1621 tggttgcaat atatctataa caaggtttat atctaatttt aaagaaagat taagaaaccg
     1681 ctctgcacaa caccacatgg atccgggcgc agcaggtgca gtatgtgcct tcgattcctg
     1741 gaacacctca aaacaatgta ttgtacatgt tttgggcgcg cggtgtggtg cgggaggtat
     1801 caggcacagt ggagattgaa ccctccttac ggagggcagg tgtcgatatg gagttgtgct
     1861 tggcacaacg ggacgacagg agctggatct gatcgagacg ctgggccaac gccagtgcag
     1921 cgcgcccggt ttccgggata aatcttcccg ggagcgtcag agagtcagga tctattagcg
     1981 acgactggaa tggacccggt gcacaccggg gctgtagcag agaacggcag gatattcgga
     2041 gattgttctc tttgtattct ccatgctctg ctaccttccg tcaactgttt cccttccacc
     2101 ccacagtttc cgggttggcc ggaagggttg ccatgccggc acgtccggca cggaagcccg
     2161 gcgcaggcat cgctctgcaa cagagtggtg cgtcacgcta taaccacgaa gggttgattg
     2221 aaacatgccc aagattaaca gaccacgcag aggatccctc gcgtatagcc cgagaaagcg
     2281 ggcgaaaagc ccggttcccc gatacggctc atggcccgat tacacgggaa ccccggcggt
     2341 gcagggattt gcaggttaca aagtggggat gacccacgtc atcatggtcg atgaccacaa
     2401 gagcagccct accgaaggca gggatgtgat ggtgccggtg accgtgattg aggtcccgcc
     2461 catgcgggtg gcaggcgtgc gcgcatacac cgaggatacc tatggtaagc gtgcgctgac
     2521 cgaggcgtgg gcgactgatc ttgacaggga ggtatcccgc aggatcaatc tcccgaagaa
     2581 ccatgatacc gccgccgccc ttggcgccat caaaaccgcc atcagcgaag gccgtgtagt
     2641 tgaactctac gctctgacct acaccaggcc agcggagatc accggtgtcc cgaagaaggt
     2701 cccggatatt atggagatgc gtatcgcggg ggggagcctt gaagatcaga tcgcatacgc
     2761 tgcagatatc cttggcgggg agatcgcgat ctctgaccat ttcgaggccg gcgagtatgt
     2821 cgatgtcacc gccgtcacca ccggcaaggg tactgaaggt cccgtccggc gctggggtgt
     2881 tcaggtcagg aagcggaaac actcccgtgg cggcaagaaa cgtcacatag gtaaccttgg
     2941 cccgtggaac ccgcatcacg tcaggtggca ggtgccccag atgggtcaga tgggctacca
     3001 gcagcgtacc gagttcaaca agcggatttt gaagatcggc gcagacagca acgatatcac
     3061 acctgcaggc gggttcctgc agtatggcgt cgtgcgcggc ccatatgtgc tgatcaaggg
     3121 ttctgtgccc gggccaagca agcggctggt caggatacgc tccgcgatac gacagggtga
     3181 acacgttgtc cgcacgccga cgatcggtca catcagcgtg cagagccagc aggggtgatc
     3241 agcgatgaaa gcacaggttc gaacattgac aggcgagatc gctcatgaga tagatctccc
     3301 ggcagtattt ggcgaggatt acaggcccga tctgataaag cgggcggtta ttgcactcca
     3361 gagcaccagg ttccagccgc acgggacaaa cccgtatgcc ggcatacgca cctctgcaga
     3421 gtcctgggga agcggccgcg gcgttgctca gataccgcgc ataaagaacg gcagcagggc
     3481 tgccagagtc ccccaggcaa ccggtggccg cgcggcgcat ccgccgaagg tcgagaaggt
     3541 tcttgtcagg cagatcaacc ggaaagagaa gcgcaaggct ctcaggtctg ccatcgccgc
     3601 cagcacccac cctgatcttg tccaggcgcg cgggcacctt tttgagggca gcctcccgct
     3661 ggtcatggaa gatcggtttg agggtctcac ccggacaagc gacgtaatcg ccgctctctc
     3721 ggcggtaggc atctacgccg acgttgagag ggcgaagggc agccggaagg tccgtgctgg
     3781 ccggggaacg atgcgtggcc gccgttacaa gcagcgcaag agcgttctca tcgtcaccgg
     3841 caccgaaccg ctccgtgcgg ctagaaacct tgcgggtgtc gatgccgtgg ccgtaaacca
     3901 gctcaacact gagctgctgg cgccaggcac gcaggccgga cgtcttacgg tctggacaga
     3961 atctgcaatc aggagactgg aggagttctc atggtattaa aacacccgtt tgtcacagaa
     4021 aaagcgatgc tgatggtcga aggcgggaac gaactccagt tcatcgtgga gagggatgcc
     4081 agcaagcagg agatcagacg cgcgctggag tcagccttcg ggcaggaagt gcgcagtgtc
     4141 cgcacaatga tgaccacaaa aggccagaaa aaggccattg tatcgtttgc ggacgcgaaa
     4201 gcggctgaag agatcctcag ccggcttgga ataatgtaag gtggtggacg atggcacaca
     4261 ggattatagc gcagaaccgc ggaaagggcg gtccaacata tcgtgcaccc tcgcaccggt
     4321 acaaggcgcc gctgcggcat gcaggaaaga atgattccct catcaaggga cttatcgtcg
     4381 atatagaaca cgatcccgcc cgtcatgccc cgattgcact tgcacggctt gaggacggcg
     4441 agaaggtcta catgctcgct acagaaggac tgggtgtagg ggatacagtc tcctggggca
     4501 gcggtggcgc tgtgaagaac ggcaacaccc tcccgcttcg agagatcccg gtcggtgcat
     4561 acgtctgcaa catcgaggcc cgtccagacg acggcggcaa gtttgtccgt tcaagtggtg
     4621 tccaggcgct tgtcatcggc aaggccgatg acggcagagt cggtgtccgg atgccgagtg
     4681 gcaagaacaa gtggttcaac ggcggctgta tggcaaccgt cggtatcgtt gccggtggcg
     4741 gacgcggcga aaaaccattc gtcaaggcag gaaagaagta cttccacgtc agatcatccg
     4801 ccgagaagtg gccccgtgtc aagggtgtat gcatgaacgt catcgaccac cccttcggcg
     4861 gcggtgggca ccagcactgc ggacggccaa agaccgtctc ccgtggtaca cccccaggca
     4921 ggaaggtggg gcatattgcc gcccggagaa ccggtaagtg gaagaagtga ggtgtgaagc
     4981 atggcaaaga aaacacagaa gcggatgccg cggaggcgcg aggagttcac ctaccgcggc
     5041 cactccattg cggacctgca gcagatggcg ttatcagaac tgctgccgct catgccggcc
     5101 agggcgcgca ggaagttcga caggggtctc tcccgggagc aagagaaact ccttgccgat
     5161 attcggtctg gagaggcacc cctacgcact cacctgcgcg acatggtcat catgccagag
     5221 atggttggga agaccgtcga gatccacaac gggaaagagt ttcagaaggt tgagatccag
     5281 ccagaggcgg tcttccacta tcttggagag tttgcattga cccgtaagaa ggtctcccac
     5341 ggcagcgccg gtatcggtgc gacccgttcg agcaagtatg tcccgctgaa gtgatggaaa
     5401 tggcaagaac aggttattcg acagagattg aaggcgagaa cgttgctaga gcaaaggcga
     5461 acgaaattcc cgtctctccc aagcactcga ttgagattgc caggttcatc cggaacatga
     5521 ccacctctga ggcaaaggcc tacctcgcag atgttgtggc gctgaagaaa cccatcccgt
     5581 tcaagcgatt caagaggaac gtcgctcaca agcgcggtct ttcaaaatgg cctgccggcc
     5641 ggtatccggt caaggccgcc gaggcctaca tccggctgat cgagtccgtc gagaagaatg
     5701 ccgagtacat cggccttgac tccacaaatc tccggatcga gcacgtagcg gccaaccggg
     5761 gccgtggttt caggtcattc ttcccgcgtg cgatgggccg cgccaccccc aagcgccgcg
     5821 agaccgtgaa catcgagatc atcgtgaccg aggtggcgta atggcaatcg agaagagatt
     5881 tatcgcggat ggcgtgcgca acgtccgggt tgagaagttt ctcatgaagg aactcaagcg
     5941 agctggatac ggtggcatgg atataacccg gacaccgctc ggcacccagg tgaccatctt
     6001 tgcggagaaa cccggtatag tgatcgggaa gggcggcaaa caggtacgcc agctcaccca
     6061 ggaccttgca agcgtctatg ggatcgagtc accacaggta gaggttcaac aggttcagaa
     6121 tcccactttt aatgctcaga tcatggcgga aaggctggca aacgctcttg agcgcgggtg
     6181 gtacttcagg aaggcggggc agagcacaat ccggcgtgtg atggattccg gcgctctcgg
     6241 ttgcgaggtt gtcgtcgccg ggaaactgac cggcgctcgc tcaagaaccc agaagtttat
     6301 tgagggctac gtcaagcact gcggcgaacc cagtgagacg atcgtcgaga agggctatgc
     6361 aattgcgata aagaaacttg gcgtcatcgg tgttcaggta aagatcgttc cgcctgatgc
     6421 aaaacttccc gatactttcg aaattatcga gcctgagccc aaggaaattc cggcgcagcc
     6481 cccggtggtt gagaatgctg atgaagatga gtttgaggag atgcctgatg acgattacga
     6541 ggaggagata taaatggcaa tctttcgtgc acacgaagtg aggcagttct ccgatatcga
     6601 actcctcgaa caggagcaga aactctccct tgaactgatc cgggagcggg gcaaggtcgg
     6661 tgcaggcgga gccaccgagg atcccgggag gatccgtgag atccggagga cgatcgcgcg
     6721 gatcaagacc gagcagaacg cacgacggag gaatgcatga tatctccccg gaacatcctg
     6781 cggcacgaac tgatcggcct ggatgttctg gtagttcgtg cgagcaaccc ggctcatgtg
     6841 gggctgtccg gtcgcgtcgt tggtgagacc agaaacaccc tgctcatcca gaccgggcga
     6901 ggggaaaaac gtataccgaa gcggttaagc gtcttccgcc ttcggcttcc cgatggcgcg
     6961 accatcgata tcgatgggtc gagtatcgag atgcagccgg aacggcggat cagcatgcgc
     7021 attaaataat cgaggatgaa taatggcacg aaacattggg ttgggcgtcc ccattccgga
     7081 aaaggagtgt gaggacgcaa attgtccgtt tcacggcact ttgccggtac gcggccaggt
     7141 gattaccggc aaagtcgtga gcgatcgtat gaacgctact gttgttgtgg agcgtgagta
     7201 tctccactac gtcaagaagt acaatcgcta cgagaagcgc cggtcgagat accacgccca
     7261 caacacgccc tgtctcaatg ccagggtggg cgatcaggtc aggatcgcgg aatgccgtcc
     7321 gctgtcaaag accaagaact ttgttgtggt tgaggtgatg aaaaaatgaa ggcgatgcag
     7381 gcgaagatcc cgcgtgccct tgccaccggc tccaggatgg tctgctcaga caacaccggt
     7441 gcacgggttg tggaggtgat ctcggtcgat ggttaccatg gtgttcggcg gcggcagccc
     7501 tgcctgggca ttggcgatat cgcaaccgtg agcgtcaaga aaggtactcc cgacatgcgg
     7561 cgcaaactcg agaaggctgt ggtcatcagg cagaagaagg agatccgtcg tccaaacggc
     7621 atccggctct cgttcgagga caatgccatg gtgctcatca acgagcgcgg agagccgaag
     7681 ggaaccgaga tcaagggtcc agttccccgc gagatcgcgg agcgtttccc gaagatcgcc
     7741 tccatggcga cgatgatagt gtaaggtgtg gtaaggatgg tacgcataac aagcaaacag
     7801 ccaaggaaac agcgtaaagc gcggtataac gcccctaacc acaccagggg gaggttcctc
     7861 accgcctccc tctctcctga actccgggag aagtacagca cccgccgggt tcgtgtggtc
     7921 agcggggaca ccgtgaaggt tttccgtggg gacttcgctg gcgaggaggg catcgtcgac
     7981 gcggtggata tgaaggcgtg ccgcctcgtg gtccacggtg tgatggtgac gaaggccgat
     8041 gggaccgagg ttccaagacc ggttgatcca tcaaatgtgc agatcacgaa gctcaacctg
     8101 aaagacagac tgcgtgaaga gagacttggg ggcggtgcat aatgtccggg catctcaagc
     8161 ggctggcagc gccggggtca tggcgtgttc cggcaaaaac aaagaaattt gtcatgaaga
     8221 ccgctcccgg gccgcacaac gccgatgccc ttccggtcgg tgtctggctc cgggagcacc
     8281 ttggcctcgc ccagaacgcg agcgaggtcc ggaagatcct gcaccagcag gacgtcatcg
     8341 tcaacggtcg ggcctgcaag aacccgcaga tgggccttgg ggtctttgac atagtctcga
     8401 tcccgaagat cgggaagcat taccgtatcc agctggataa gcggggcaac tatgttgcgg
     8461 tcggcatccc ggcggagtct gcaaagaccc gtctctgcag ggtcaggggc aagacgactc
     8521 tcagcggcgg cagggtgcag ttgaaccttg gctttggagc aaatatcctg gccgacaata
     8581 cctacagggc aacggactcc attgtggtga cccttggaat agatggtgat gagcgtttcc
     8641 agatcgtcga tcacttcccc tttgcagaag ggaacgtggc catgatcata ggtgggaaac
     8701 actcgggtaa ggtaggcagg atcgtcgaga taatcaggac ggcaagccct ctcccgaatc
     8761 gcatcgtcct ggtggatgat gcgaccgatg agcgcttcga gactattgag gactatatct
     8821 ttatggtcgg tcgctccgcg attgcgcctg aactggaggt ggtctcatca tgagcaatcc
     8881 tatgcaggag atctacatcg ataaggtcgt cgttcatatg ggtgttggag agagcgggga
     8941 gaggctggtc aaggcggaga agatcatgca gcagatcacc ggccagaaac ctgtccgcac
     9001 catagcaaag cggacccagc cggcgtttgg tatccgtaaa ggggcaccca taggctgcaa
     9061 ggttacgctg cgccgcgaga acgccgagaa gttcatcacg accgcccttg atataatcga
     9121 taggcgactg gcgccatcac agtttgaccg gaccgggaat gtctcattcg ggatcgagga
     9181 gcataccgat tttccgggca tgtcctacga tccgatggtc ggcatctatg ggatggacat
     9241 caatgtggtg ctagagcaca agggtgtgcg ggttgcacgc cggcgaatcg agcgaaggaa
     9301 actgccagag acgcagaaag tgagcagaga tgatgccctc gcgttcatgc gcgagcgcta
     9361 cctggtggag gtttaaggca tggctgaaga gtcaggagcg cccgcatctg gcaagggcgt
     9421 caagaaattt ggtcgcgggg cgaacgagtg ccgcatctgt ggccggaagc aggggctcgt
     9481 acgcaagtat gacatctact tctgccgtca gtgcttccgt gagaatgcaa ggaagatggg
     9541 ctttaagaag ctgaactagg ggtggagaat atggcacgac tgaatccaat cgccgacgcg
     9601 atgtgtgcga ttaagaatgc cagtgatgct ggaaagagcg aggtcattgt tgagcccgcc
     9661 agcaagctac tcggtgctat gctccgcgtt atgcaggaaa acggctttat caacggcttt
     9721 gagtttatcg atgacggccg tggcggccag tttcgggtcc agatctccgg cacgatcaat
     9781 aaatgcggcg cgatcaaccc gaggtttccg gtggcgatgg ccgagatgga atactgggag
     9841 tcgcagtacc ttccggcgaa gaacttcggc atcctgatca tctcaacctc aaaaggtgtc
     9901 atttcccaca cgcaggcgcg gaatgagggt atcggcggag aactgctggg atacgtctac
     9961 tgatggaggg cgagagtatg ggaataacac gaaaggtcag gatcccttcc ggcgtgaaca
    10021 tcacgctcga aggcggtgtg ctcaccgtct ccgggccgaa gggcagactg gtccgcgata
    10081 tgcgcttccc gcagattgac ctcgggatcg aggatggctt tattgtcatc tcgaccgcgt
    10141 ctgacaagaa gcggttcctt gccatgagcg gcacgcttga agctcacgca gagaacatga
    10201 tccggggtgt tgttgagggc tacgagtacc ggatgaaggt tgtatacagt cactttccga
    10261 ttcagctcaa gttgaagggc gaccgtcttg agatcaacaa cttcctgggc gagaaacaac
    10321 caaggttggc aaagatcctg gacggtgtca ctgtaaacct cggaaacgac gaggtggttc
    10381 tctcaggtat cgacaaggag aaggttggca acacagccgc aaacatcgag catgcaacca
    10441 ggatcaggaa gcgcgatcca agggtgttcc aggatggtat ctacatcacc gagagggcgt
    10501 gagaatcatg gatgaaaaga gaagattgat ccgcgtccgc accagtcaca ataagccagc
    10561 cttcaagagg cgggggcttc atcggaaggt gaaactggca gatgtctgga ggcgcccgcg
    10621 tggtctgcat aacaaacaga gacggcagtt tagggcaaaa ggcgcccttc ccggccctgg
    10681 ttatgggggg ccgaccgcag tccgcggcat gcacccgagt gggtatgagg aggtgcgggt
    10741 cttttcacct gcggatcttg acgggttgaa ccctgagact caggccatcc ggatcgctgg
    10801 atctgtgggt aacaggaagc gaggcgtgat ccaggagagg gccatggaga tggggttaaa
    10861 ggtgctgaac gccaaggatc tcacccgcgt ggccggggaa gccgtcgcgg ccgaggaaga
    10921 ggtgagcgag gatgagtgat ctcggcaacc agagacgaat ggcggccgcc atcctcaagt
    10981 gcggggtcaa ccgtgtctgg ttcgatcccg aacggcaggc cgatattgag gcggcgatat
    11041 ccaggaagga tctgcgggat ctcattggtg aaggtgcgat ccgggcaaac cccgtgaagg
    11101 ggaacagccg tggcaggaca agggcccgta tggcgaagcg ttcctacggt catcggaaag
    11161 ggtcaggtcg gcggaagggt gcggccggat cacggaaccc gggtaaacgt gcatggattc
    11221 gcaagatcag ggctcagcgc cgtctcctgc gcgagatgcg tgacgagggc acgatcgacc
    11281 gcagcgtcta ccgccttatg taccggcggg catccggagg acagttccgg agtgtcgcgc
    11341 acctgaaatc ccacgttgat atgatggcag gagggatgga ataatggcaa ccggtccacg
    11401 ttactttgtg cctttccgca gacggcacga gggaaagacc gattactaca agcggatggc
    11461 gcttctctca tccgggatgc ccagaatggt cgttcggaag acaaaccggc agatcatcgt
    11521 gcagttgatc gttcccgggg ttgagggtga ccgcaccctt gtgtctgcct actcgacaga
    11581 acttgctggc tacggttatg agggatcagg cgcgaacaca ccggcagcct accttaccgg
    11641 gatgctgttt ggtgtcaggg cgctgaacgc gggttatagc gaggcgatcc tggatatagg
    11701 gcttgccagg gcaaaaccgg gcgcgcgcgt ctttgccgct ctcaggggag cagttgatgc
    11761 gggtcttaac gttccctatg gagaatcgat cctccctgcc gaggagcgac tcaggggcgc
    11821 tcacatagca gagcatgatc ccgatcgggc tggtaacctg gtggagaacg tagaggctgt
    11881 ggctctggcc atcaagaagg agctggtgtg aaaatggcat acgtagagga agaatggatt
    11941 ccgctcaccg gcctcgggcg cagggtcgcc gctggcgagc tcaccagtat cgatgaggtg
    12001 ctcgcaagcg gcaggccgat caaggagccc cagattgtgg atgtcctcct gcccgacctt
    12061 gaggacgagg tgctggacat caacatggtc cagcgtatga cggactcggg ccggcgcgtg
    12121 aagtttcgta ccaccgtggt ggtcggcaac cggaacggtt acgtggggtt cggtcagggc
    12181 aaggacgtcc aggtcggtaa cgcgatccag aaggcaatag cagatgcaaa actgaatctc
    12241 atcaaggtct cccgcggctg cgggagctgg gaatgcggtt gtgatgccgc ccactcgatc
    12301 cccttcgagg tgaccgggaa ggcaggcagt gtccgtgtta cgctgaagcc cgccccacag
    12361 ggcatcgggc tggttacagg ggatatcccg aagaaggtgc tggcgcttgc cgggatcaag
    12421 gatgtctggg cgttcaaccg tggagagacc aggacgacca tcaactatgc gaaggcaacg
    12481 tttgaggcgc tccggaagac caacatggtc aggatcgggg gtacggagtg atgtacgcgg
    12541 tagtacaggt gcgcggtgtg gtcaagacca accgcgagat cagggacacc ctgaagatgc
    12601 tccgtctcca ccacgtcaac cactgtgttc tcgtgcctga aacacctgca tatcagggca
    12661 tgatccgcaa ggtaaaggat tttgtggcct acggtgaggt ggatgaggag accctcgcca
    12721 cgctgctgcg cacccgcggc aggcttaccg gtgatgagag gctgacggac gactacatcc
    12781 gtgagcacac gccttatgcc gatatcgatg agtttgcggc agcgctttgc agcggcgaga
    12841 cgagttttgc tgatctggtg gagatcaagc cggtgttgcg gttgcaccca cctcgaaagg
    12901 gctttaaaac aatcaagaga acctttcagc agggtggggc tctcggctac tacggccccc
    12961 ggatcaacga tctcctctac aggatgaggt gaatctgatg cccgtaaaca agagatctaa
    13021 atacaggggt tcacggacat gtggcggcgg tacgcacaaa aaccggcgtg gcgccggaaa
    13081 caggggtgga cgtggcagag ccgggcagcg tgatcatcgc ttctctcatt tctacctgag
    13141 gggggagata tccaacggca agcacggttt cgtcaacaag acttccgtgc cggtatccgc
    13201 cattgacgtt ggggatatcg accagatgat cgaggcgctg gtcagcgaag ggtttgccac
    13261 ccgggagggt gatcttatca ccctcgatgc caccgagatc ggcatcgaga aagtgctcgg
    13321 cggtggacag gtgacccaca ggatgagtat ctccgccagg gcgttctcgg aacgggcccg
    13381 ggcaaagatc gaggagatgg gcggtcaggc aatgaccgtc tgatcctctt ttaaggtgaa
    13441 accatgggag atctgctgga tcgatttgaa cccatccttg cagcaatgcc tgcagtccga
    13501 aatccggagg ggcatgtcca ttttaagaac aaacttatgt ggacggttgc gattctgctc
    13561 ctctacttca cgttgacgaa catcgatata tttgggctct cgccgcagtc acaggatctg
    13621 ttcggaatgt accgcgccct gcttgccggt gcgagcggtt cgcttctgca tctcggtatc
    13681 gggccgatag tcaccgcgtc aattgtgctg cagctgctca agggtgccga cctcctgcag
    13741 atcgatacca gtgaagcccg cgggcaggtc atgtatatgg gcctgcagaa gatgctcatc
    13801 ttcgtgatga tcgtgatcga ggggctcccc atgatcgttg gcgggatgat gctgcccgat
    13861 ccggcggtgg ctgcggagtt ctttggcggg aacacgttca tcgtctcggt cctgatcttt
    13921 ctccagatct gccttggcgg attgctggtg gtcttcatgg acgaggtcgt caccaagtgg
    13981 ggcatcggtt cgggtgtggg gctcttcatc gttgccgggg tctctcaggg tctcgtgaac
    14041 gggttcctga actggcagac aggaaccgat cccttcccga tcgggttctt cccgcggctg
    14101 tttgcggtgg cgacttccgg ggtgaacttc cttgagtact tcgggacgga cctgctcgct
    14161 ctcgttacaa cgatcgccat cttcctggta atcgtctacg tggagtcgac gcgcatcgag
    14221 atcccgcttg cgcataccgc tgtccgcggc gcccgtgcgc ggttcccggt taaactcatc
    14281 tacgcgagcg ttctccccat gatccttgtc cgtgtgctgc aggccaacgt ccagatgatc
    14341 gggatgctgc tctcgaacgc cgggatcacg atactgggcg agttccaggg acaggtgccg
    14401 gtaaacggtc tgatgtggta catcgctcca ataaaccagc cgcaggactg gatgtggtgg
    14461 atcgctgacc tggggcatgc tccatgggag atcctgcttc ggatgggaat agatgtcggt
    14521 gtgatggtta tcggcggcgc gatattcgcg ctcttctggg tcaagaccgc tggtctcgac
    14581 tcgaaggctg tggcccggca gatccagagg agcgggatgc agatcccggg ataccggagg
    14641 aatgttcagg tgctggagaa gtacctcgac cgttacatcc ccaggattac cgtcatcggc
    14701 ggtgtcttta taggcctcct ctcggtagtg gcaaacctct tcggtgtgat cggggcggtc
    14761 ggtggaacgg gattgctgct tgcggtgagt ataacctacc gcctctacga ggagattgca
    14821 agccagcaga tcatggagat gtacccgttc atgcgcggtt tcttcgggaa ggagtagtta
    14881 tgatgaccct gcccggcggg cggggtcgga gaactccctc ctccgggaga acccaaagtg
    14941 agttccacgg taactcgtcc tgaccgcctc cctcaagacc gaatgacgac tttttttttg
    15001 gttcggtggt gccgggcggg ttccgaaagg cattactctt cgaggcacgg atctatcttg
    15061 cagagaaaaa aaatacccgg attggagtga taacgttggg aaagaaagtc gttgtaaccg
    15121 gcgtccccgg tgtagggaag accaccgtca tcaacggggc gatggagagg ctggcggccg
    15181 agggcatcgc ttatgaggca gttaactttg gcacatttat gtttgaggcg gccaggaggg
    15241 agaacctggt cacggatcgc gacgagatgc gaaaacttgg taaagatgtc cagaaacgcc
    15301 tgcagaaaga ggctgccgca ggcatcgcag ccatgagcgt agatgcaagc gttatcatcg
    15361 atacccatag cagcgtcaag accccggcag ggtttcttgc cggactgccc gaatgggttc
    15421 tccatgaact tatgcccgac atcatcgtgc ttgtggagac cgatccagac cagatcctga
    15481 tgcgtcggct gggtgacccc tcaagaagcc gtgacatgga gggttcgcgc gcgatcgccg
    15541 aacaccagga gttcaaccgt gccgttgccg ctgcatatgc gatgtatgcc ggctgtacca
    15601 tcaaaattgt caggaacgag gatttcctgc ttgagcatgg catcgatgac ctggtgagtg
    15661 ttttgaggta acccgatatg gttgacctga agaagcaagc ccccacgatc gcacttctct
    15721 ttaccctgct ggttttcctt tcatacagcg tcagctgggt gcgcctgacg atcggtacag
    15781 caatggatgt tgtgcttggc cctctcatcg atcctgaggg gtttgcggtg ccgttttttg
    15841 tcatgatcat catcctctcg agcctgacag gcctctattc atcgctcgtc cagaagtaca
    15901 ccatcgatta cgagaagatg caggagacgc aggcgaagat gcgggtcttc cagaaagagt
    15961 tccgggaggc gcagctctcc ggggatgaga agcggatcaa gaagctccag aagaagcagg
    16021 agcagatgat gcaggatcaa ctcgagatct cccggcagca gttcgtcccg atggcgatca
    16081 tccttttgct gacggtgccg atcttcttct ggatcctcct gcggcttccg gcggtagggg
    16141 cagcggctga tattgcaacc gggatcgtga tgccgtttgc agggattgtc agcctctccg
    16201 ccactgtact ttggtttata cctgcctgga taatctggta tatgctatgt tcacttgcga
    16261 tgagccaggt gatccgcaag tctcttaaca taggtgggat ctgatgcgga tcaccatcag
    16321 cggcccgccg gggagcggga caacatcgct tgcacgctac cttgccggga aacacgggtt
    16381 tgacctgatc tctgcgggag aggtgttccg gcaacttgcg cgggagcacg gcatggatct
    16441 cgcggagttc gggaagtttg cagagagcga cccatcggcc gatcgcatga tcgatgcccg
    16501 gcagaaggag atcggggagg ctgccgatga tatcatcatc gagggtagac tctcgggctg
    16561 gatgatcgtg aacgccgatc tccggatctg gctcaaggcg tcgcctgaat gccgggcaaa
    16621 gcggatcgcg gaacgcgacg ggatggatga ggaggcggcc cgggtctata ctgagaatcg
    16681 gcagcgctca gaggccacac gataccgaaa ttactacggc atcgagatcg atgacctctc
    16741 tccatacgat atcgttctgt cgtccgagac tttcggtgtt gaggctcttg gggccatcgt
    16801 ggataccgcg atcgagtgtc ttcggcggca aagaggcgat gtagggcagt gagccctttc
    16861 atcagtttct tctgccgcaa cctcttctct tctcactttg ccaccattcc agaataccgc
    16921 cgcaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttg agaaggcgat
    17041 tcaaagagcc aggtttttat cccggcaaat ccacaccctc gcatatgagc gagggtgaag
    17101 gtcgctttgt aaaggggcgc tgggttactg acccggcccc ggtacctgaa tcagaaccgg
    17161 aatcggcacc ggttgcccct acagtcgagg aacacttgca tgaggcatct cagtcggtca
    17221 aaaaggcggt cgacgatgtg gtaagcgcag gccagcatct actcgggacg ccggaaggtc
    17281 acgaacacat ccagcaggcg gcaaagaagg ccgcagagga tctcgaacgg gcgatcaact
    17341 cctgggcgga gtccgcacgt caggcgctgc agaggcggcg aggcagctcc tgacgtgtgc
    17401 gatgacctcc gggtttcgtg gcagcatgat gtggcagtag cgctcggggt gccggcccag
    17461 gttggcaggg tctgccggga ggatctccac acctgcgccc gggaggtgcg agtcggagtg
    17521 agggacgatg ccgtcgccgg catacgtcat tctccagcct tctccgggtg caaactcaag
    17581 tgtcctgccg ccaaaagccg ggaagaacgc aggtgttctg gtgaggtttg cggccaggat
    17641 gagcctgtag gctatatcgt cgcgggtgcc tgcccgggag agcgccgagg tgaccctgct
    17701 cccagggcgg aactcctgga cgatcaggtc ttccgtgggg tcgtagccgc gcggcacgaa
    17761 gacccctgca agccgccgga tcacctccgg gccctgttcc gggtcgttga aggcctcggc
    17821 catcgctgaa cctttgttcg gagggccgat ccccacaagg aaccggacat cctcctcgcg
    17881 gtcgctgccg tcgatgacct ccaggaggta acgggcgata catgttccca tcgagtggca
    17941 gacgatgtcg acggggccgc catagccgtt ctccttcctg cgtgcacgga tgaactcctg
    18001 caacgccagg gctatcgcct ccgttccatt ctcctggatt gcggtatggt caaacttcca
    18061 gcaggggagc ccggcatcct ggagtgctgg ggcgaggtgg ttccatattc cgggatggct
    18121 ccgccagccg tggacgagga cgaccggatg tctgtgatcc atcagcataa ccctgcaacc
    18181 tccttctgcc tgcgacggtt catccttcag gtgtttgaca ccccgccaca atgtaggttt
    18241 cggcaggggt gtcagccgcc agccatcaag tttgccttca ggaacgcacc tgccggcata
    18301 ggatcggcgt tttgcatctt cgcgcgccga atcagtcgcc ctaacaggat cggtgtctgg
    18361 ctcgatctcg caaaggatct gtggttttct ggaggatgag tctcagtaga accatcatcg
    18421 gaaagtcgtc atctgatagt ccggcatacg ctgcccgccg tagtgattgt ccttccttgt
    18481 cgatgaagcc gggtttgaat agatgtat
//
LOCUS       DAOSZX010000021        18119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2556_length_18119_cov_19.429307, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000021 DAOSZX010000000
VERSION     DAOSZX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18119
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2556_length_18119_cov_19.429307"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..565
                     /gene="cbiT"
                     /locus_tag="PK196_03260"
     CDS             35..565
                     /gene="cbiT"
                     /locus_tag="PK196_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HON40473.1"
                     /translation="MGLRAGPTQDEVMAVSLAKLGIRPGDRVVDVGCGTGKVSIAASK
                     TAGTVYAIDRRAEAIACARQEAAAAGAGNIEFFEGDAVDVLPDLGRLDAAFVGGSRRL
                     PEVLDILARMVQGRIVVNAVMVGTLHEAIVSMQRLGIFIEAVHIQVSRSAGIAGGVMF
                     KPLNPVYIIVGGAGCS"
     gene            556..1173
                     /locus_tag="PK196_03265"
     CDS             556..1173
                     /locus_tag="PK196_03265"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HON40474.1"
                     /translation="MLVGVGLGPGDPELLTLRAVRLLREAKAVFVPGKLAYDLVRPYR
                     ADAVVLKFPMTNDEGYIRRCLEENADLIAPHAMDGLAVFGILGDPNFYSTFSRLAGVI
                     AERHPGVSFATEPGVSSITAFASVANVPVSGRVLVSDGNGEESRVIMKVRRPAALAAD
                     LREEGFSEFVLVERMFMPEERVYRGDELPEESDYFSILFARRSHE"
     gene            1166..1882
                     /locus_tag="PK196_03270"
     CDS             1166..1882
                     /locus_tag="PK196_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HON40475.1"
                     /translation="MNKVYIVGAGPGAPDLITVRGMDLLRKADVLIYAGSLVNPALVE
                     ESGAALKLDSQNMKLDEIVGAIEEHVRAGGLVVRLHSGDPSLYGAIVEQMAELERRGI
                     EAEIVPGVSSLFAASAALRTQFTLRDVSESLVITRPAGATLERDLIPEFSRLGQTMVV
                     FLGTERMEEVLARVECPPETPAAVVYHASWPDEKVVRGTVADIAGKARAAGIERTALI
                     IIGRVVDPATSGFRRSVLYS"
     gene            1879..2751
                     /gene="cbiG"
                     /locus_tag="PK196_03275"
     CDS             1879..2751
                     /gene="cbiG"
                     /locus_tag="PK196_03275"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HON40476.1"
                     /translation="MTGTVVVALDRFLPSARRIAETLGADLVAYTSGVFADLFGRYDR
                     IVALMSAGIAVRGVAPLLVEKWRDPAVVVVSPDLRYAVPIIGGHHGGNDLARELAGIG
                     ITPVITTATETQGRDSVEGIAARSGCDIVNRDSTRAVNAAMLDSDLPLYAIRGPAIVV
                     AGPGVSILVRKGDYVVGIGCRKGAPAAAVAGALRQALEQAGIAPEDVFVYATTAKKRG
                     EAGLRDAVAELGGNLVYLDDDTLNAERAPSPSRASLIGLAGVAEPAALAISKRKELVL
                     AKQTYGCVTVAIAR"
     gene            2720..3514
                     /gene="cobJ"
                     /locus_tag="PK196_03280"
     CDS             2720..3514
                     /gene="cobJ"
                     /locus_tag="PK196_03280"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HON40477.1"
                     /translation="MDVSPLQSHDKRGKLYIVGTGPGDLLHITPRALKALAEAECVIG
                     NGIYLDLVMPVIAGKEVIRSSMGREVERASRALDLARDRRVAMVSGGDAGVYGMAGIV
                     LEVAESSGSTVEIEVIPGITAALSAAARLGSPLSGDYVTISLSDLLTPLEEIERRLDL
                     AFQMGVPVVLYNPRSRGRPHNLETAIGIARRHLPDETPVGVVRNAYRDGEERLITTLG
                     EFDGHISFVDMHSIVFVGGEETRIWRNEADERGIITPRGYHRKYVY"
     gene            3462..4151
                     /locus_tag="PK196_03285"
     CDS             3462..4151
                     /locus_tag="PK196_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HON40478.1"
                     /translation="MREGSSPRGDTTGSTYTDLAAITPEAYAIASASRSLARERLGNA
                     TPEDRIRQRCSIAVGDFAMADLMRFSGDPVEAGVAALERRAPVIVDIRMVQAGVLRRG
                     HSSEIICALDHGEGIAMERGITRTSAGFLALRDRLEGSIVVIGNAPSALLVVCGMIRE
                     GVHPALVVGTPVGFVNAAESKEELRALGVIPSVSNLGTRGGTPVAVAAVNEIITIYAE
                     RGGHAGSGHWV"
     gene            4126..5139
                     /locus_tag="PK196_03290"
     CDS             4126..5139
                     /locus_tag="PK196_03290"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HON40479.1"
                     /translation="MRDPVTGFEYPDEWVAACRSPELLDDVQRGLAVLTASGLVLRRG
                     FSTGTTAAAACKAAVLSLALDTVKEVEVRLPCGIAVRLPVDAYRGQASCWKDAGDYTA
                     DVTAGLEFVATAAPSLNDGAHFVPGEGIGSFDRDTPRHRQGTPAISATALECIRRSID
                     EAMEETGLSGATIILTVPRGAEVAQKTLNPKVGVHAGISVLGTTGLVEPWDDHVTEGV
                     LERVANADAVVLTTGRLGLRYSRLLFPEREAILVGNHLAEALQAAAGGDPIVCGLPGL
                     ILRFMNPQILEGTGCATVEELMATPGGEEVLRRELEAFRSRYPGVRVVIVDRGGRVIG
                     ESP"
     gene            5136..>5683
                     /locus_tag="PK196_03295"
     CDS             5136..>5683
                     /locus_tag="PK196_03295"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HON40480.1"
                     /translation="MKIVGVGCGPGMLTAEAVRVLKDASVVYGSARAIALARDAIPPG
                     CEVHGITDYRSLRSLPRDAVVLSTGDPMLAGLGPLLEGDIVPGISSLQVAFARLRLPL
                     ARVAVVRAHGNDHDHAVASAIEEIERGRIVFIIADPAFEVEGLAALLPPAARVAVCED
                     LGYPTERIATGTAAEPPSPRGDL"
     assembly_gap    5684..5783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5784..6614)
                     /locus_tag="PK196_03300"
     CDS             complement(<5784..6614)
                     /locus_tag="PK196_03300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON40481.1"
                     /translation="MRSEVIYGNTFYNGDCISGAAAHIPDDSIDLIITDPPYGINGDR
                     LHRHYNRDERFVIDGYVEVAAADYGEFSRRWIAEAERILRPGGSIYIISGYTNLYHIL
                     HALRGTGLREVNHIIWRYNFGVYTSRKYISSHYHILFYEKPGGERTFNLESRYGALER
                     GPDNRSPNYSDREDVWCINRDYKPGRAKNKNELPAELLTKMIQYSSSEGDLVCDMFLG
                     GFSTAKAAIGLNRRVTGFEVSEEIFERKIEEIRAIRPGCLLPTLRRPETGAPRNQGRP
                     W"
     gene            complement(6734..7456)
                     /locus_tag="PK196_03305"
     CDS             complement(6734..7456)
                     /locus_tag="PK196_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HON40482.1"
                     /translation="MDDTTGVSQRVWWRTILENRYIAGPIKFLLPFGIGIILFAILYL
                     LLPYQDFLNLSGLLAAYFIPPAGKESIIPIAIMMGYHWWLVTFAIFMMDAVVSLFIVW
                     NFDLALKIPLIGGLIESGVRAGREFFEARPWVRRFSTIGLILFVFFPLQGTGAMNGSI
                     LGRLLGLEKARVFLCVCTGSFLSSLLIALGADLLLDIYRENNALGLGLLAAILVTIVA
                     AVVALRLRKKRLRERKSKAGWW"
     gene            7576..7842
                     /locus_tag="PK196_03310"
     CDS             7576..7842
                     /locus_tag="PK196_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40483.1"
                     /translation="MTSPPATRSPRNLPLIEAGSRESALCTIANVAAALETLREVRKH
                     VRGDHKRRLDDVAVILRVIAFDTQATYAITDDEAREFIRKCRPV"
     gene            complement(7820..9064)
                     /locus_tag="PK196_03315"
     CDS             complement(7820..9064)
                     /locus_tag="PK196_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40484.1"
                     /translation="MEPSTYTGENAADMDAVARFYVKCVLWLGQHDPNFVEAYFGPEA
                     VREEALTVSVPPDRVAGYAADLLATLDRIEPGHLDDPWRRRRAYLQGMIRALESRAAI
                     LAGKRPGFDAEVRSLFGIAAPEDDPDYYGGLHATLDSHLPGTGDLASRYRGFMELFLV
                     PADRLETVFAEAVAESRRRTAEHLPLPAGEHLEVAAVSGEGCSRYQGGGRSLVGVSTT
                     TPFTIDRVLAHACRHGYPGRHTIRAIREAALVRARGWIEHSVIPLASPFTTVAEGAAL
                     YGEEMIFPDRERIRFYEEVLFPLAGFDPADAGLLDTVASAAAALELAGNARAARGYLA
                     SLLSREEACRLLQDVLLLDREGAEARLRFIEEFRGYPVAIFGGCRLVREYVGSGTDRW
                     ERFARILTDPVLPGDLTPGGTS"
     gene            9169..9717
                     /locus_tag="PK196_03320"
     CDS             9169..9717
                     /locus_tag="PK196_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HON40485.1"
                     /translation="MNEPLRKAVHLIFGLAIACFILFSERKLALYALVIALFLGFILS
                     DALSRGYTIPGVSKIIATLERRDVLPGKGALFFALGALFSLTFFPKEVTFIGLVVLAL
                     LDSVTTLVGLRFGRTRIYNHKSIEATLAGFAATTAALLFLIPAHAAILTSAAAAFAEL
                     VSPVDDNLIVPAVVCIALTVLF"
     gene            complement(9714..10718)
                     /locus_tag="PK196_03325"
     CDS             complement(9714..10718)
                     /locus_tag="PK196_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HON40486.1"
                     /translation="MGAAAAEERDEILKEKTARLSVASNTLLVATKLAVGLAIGSVGI
                     ISEAIHSGIDLMAAAIAYFSVRQSARPPDECHTFGHGKYESLSGLIEAILILAAAVMI
                     IIEALRSLFGGEPTLYMEGLWSGMAVMLLSAVVNLFVSSRLMAAAKKTESIALESDAW
                     HLRTDVYTSLGVVAGLLLIRLTGLVVLDAIIALAVAVIILRAAFGLIRRSFQDLVDQS
                     LPVEEEERIREIIHGHCTDVIDFHRLRTRRSGPNRFVDLHLIVPRTATLEEAYGIVKH
                     LEDDIRQEFPRTNVTIRVQPCTGEDCSACGVFATCPECDHHSHASGDPETEGRRGERP
                     "
     gene            complement(10791..>11635)
                     /locus_tag="PK196_03330"
     CDS             complement(10791..>11635)
                     /locus_tag="PK196_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HON40487.1"
                     /translation="EDIRRAREHAARFGKSIHVIAKIERAEALANIDAILQEADGVMI
                     ARGDLGIQTPIEDVPIVQKRIILKANILGRPVITATQMLMSMTENIRPTRAEVTDVAN
                     AILDGTDAVMLSEETSIGSHPVETVAMMAKIARSTEASRPVIGAGSATLEHFRQGMER
                     ERITVNDVVSLNVIQALDALGIRYVLTPTRTGTTPRSISRFKPAAWILAFTRSPETFR
                     FLAFSCGVCPFLVKSEKDYRHGAILDMIRESGLVAPGERVVLTEGVSPGGRGTNSLVI
                     LTLD"
     assembly_gap    11636..11735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11736..11959)
                     /locus_tag="PK196_03335"
     CDS             complement(<11736..11959)
                     /locus_tag="PK196_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HON40488.1"
                     /translation="MIYHTAPGSGGRRISLPDHRTKIVSTIGPASRSCEVLVRMIQAG
                     MNVARLNLAHGTFEEHRENIRNIRAASEETG"
     gene            complement(12025..13221)
                     /locus_tag="PK196_03340"
     CDS             complement(12025..13221)
                     /locus_tag="PK196_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON40489.1"
                     /translation="MKALIETENLTYTYPNGPAALAGVSLRIDAGSKTAIVGPNGAGK
                     STLLLMFNGMLRPDRGEIRFAGRPIAYDNRSLRDLRRRVGFVFQNPDLQIIAPTVEAD
                     VAFGPLNLGLPPDAVRKAVRDALGYVGLQGYERRPPHHLSGGEKKRVAIAGILAMEPE
                     VLVFDEPTNTLDPASAEEVMELLDELASSGRTVLISTHDVELAYRWADSAILMERGRI
                     LARGSPEEVFSDPGLLAAARLKPPALLDLYNELALRGIIDGDAPPKSVLEFTDRIERI
                     IHGRAVTAERVGSVYLCDAERVCGDELRRFIESGAVEHVGAMGTRAKEFAGRERIYPD
                     YTYGVIDRCILKALIGEDSLIITSGGMVEHVKRRIGEYSAESGQKIPVIPVEEHKGRH
                     GARVTS"
     gene            complement(13218..13994)
                     /gene="cbiQ"
                     /locus_tag="PK196_03345"
     CDS             complement(13218..13994)
                     /gene="cbiQ"
                     /locus_tag="PK196_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HON40490.1"
                     /translation="MHEMLEDLARGNELRETAPWLKLLLGVASILLCVSSPAPIAPLL
                     VAASMSAAILLLAKIPARFYARLLLIPVSFAVMSIVVILFITGSGTVIIEFPGLPLAV
                     TTDGANLAILVLARVFGGMCSLYFIALTTPMTEVFDLLHRLRVPTVLIDLAMLTYRFI
                     FILIDEAGEIYRSQVMRLGYGRFRESVNSLGMLAGALFIRTWESGEALVMAMDARCYD
                     GVLAVPEENQAVSGLSLALTLAYLGAVLWVSLTTGGLRML"
     gene            complement(14009..14287)
                     /locus_tag="PK196_03350"
     CDS             complement(14009..14287)
                     /locus_tag="PK196_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HON40491.1"
                     /translation="MKHGMEIAVAAIIIIFAGVFLFQDAAGDKSWEGADGEAAELIEA
                     SGYEPWIEPFWEPPSGEIESLLFALQAAIGAVIIGYVFGYWQAGKKTA"
     gene            complement(14284..14976)
                     /locus_tag="PK196_03355"
     CDS             complement(14284..14976)
                     /locus_tag="PK196_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HON40492.1"
                     /translation="MHIMEGFLPSPWWEIWFLVSLPFIVFGVYQLNRLVKERREALPL
                     LAVAGAFVFVLSSLKLPSFTGSCSHPTGTGLGAVLFGPCITGVLGLIVLIFQAVFLAH
                     GGITTLGANVFSMGIAGPVVAWLVYKAGTRAGANTYATVFAAAALADLFTYVVTSIQL
                     ALAFPGTTGFLGAFTAFAAIFAITQVPLAIIEGAVIALVFKYIVHLKPEILIRLNLLP
                     ESTIQRLREAAA"
     gene            15105..16439
                     /locus_tag="PK196_03360"
     CDS             15105..16439
                     /locus_tag="PK196_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HON40493.1"
                     /translation="MHRIPRIVLAGTHSGCGKTTIASGLMAALAARGLAVQPFKVGPD
                     FIDPTHHSAICGRPSRNLDPFMMGEAGVRETFIRGSAGAAIAIIEGVMGLYDGLEGTD
                     IASTAHVAKILDAPVILVVDAEGASRSVHAMVRGYAGFDPEVRVAGVIINRIGSPRHL
                     AMIEETESLPIYGAVPRRNDLAVESRHLGLAMAAETGMMARYGAVVEETCDLDAVIGL
                     AASAPPLTDTGTAMPRQETRVRIGVAEDAAFCFYYADNLERLARAGADLVFFSPMADR
                     LPDVDALYLGGGYPELHAGALSSSRCRGDLRRAADDGMPVYAECGGLIYLSERLTADG
                     ADYPMAGILPATAEMTGRIQALGYVEARVAGGAPPVLAPGSLFRGHEFHYSRLECARD
                     ARFALELSRGRGIDEGRDGLAAGSAVGQYTHAYFTDEFAQTLVKAAAGFRRS"
     gene            16476..17666
                     /locus_tag="PK196_03365"
     CDS             16476..17666
                     /locus_tag="PK196_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40494.1"
                     /translation="MRAGELESLRERIAASPDAILVMVVLVIFMDMMIYGLLIPIFPQ
                     YASSLSVEESVIGVIFGLYAAMLLVFSIPMGLLSDRLGRRPLIVVGMLLLALATALFG
                     LSSTVLHLAAARTVQGISAAATWSAGLALIADTCDPARLGEKMGIALSSVGLGTVLGP
                     VAGGLLFEYAGYTATFILPAILAAVIGLLVLAVPVRTPRRESPHLLPRGALPNLAVCA
                     AVIVAVSGTYGVIDPYLPVHLHAAFSASPGMIGGMFAVLAAAAIIAQPAAGRIYDRCG
                     GSRCLIPGGLVLAGAATLAAVEAETILITAVSVFVLGVGLGSALVPVMPILAEIYRSQ
                     GSQGAAYGIYNTFYSAGLSAGPFAGAVVLARWALPTLFLLLAVILAAAGVLCWSAIGR
                     AGWR"
     gene            17704..>18119
                     /locus_tag="PK196_03370"
     CDS             17704..>18119
                     /locus_tag="PK196_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HON40495.1"
                     /translation="MFQEFCRRHTPELLIALFGLIFVAAAFGGVEGEAGAFLASALDV
                     ALFVILAMLVHLAGTRHPAYRRAAIIWLFAVVAGLAITTASLGAVAILPEEIDPDLFD
                     PELAATVALLFLGVFAAGLLSLVGLSRRFRVWLAGYL"
BASE COUNT         3120 a         5529 c         5871 g         3399 t
ORIGIN      
        1 ttagcacaaa tttctcagga taacagggat cttcatggga ctgagggccg gtcctacaca
       61 ggacgaagtc atggcagtct cgcttgcaaa actcggcatc cggccgggcg accgggtggt
      121 cgatgtgggc tgcgggacag gaaaagtttc catcgctgca tcgaagacgg ctggaactgt
      181 ctatgcgatc gaccgccggg ctgaggcgat cgcctgcgcc cggcaggagg ccgccgccgc
      241 cggcgccggg aatattgagt tcttcgaggg cgatgcggtt gacgtccttc ctgatctcgg
      301 gcggctcgac gccgcatttg tcggcgggtc gcgccgcctc ccggaggtgc tcgatatcct
      361 ggcgcggatg gtgcagggga ggatcgtcgt gaacgcggtt atggtcggga cgcttcacga
      421 ggccatagtc agcatgcagc gtctcgggat ctttatcgag gccgttcata tccaggtctc
      481 tagatcggcc ggtatagccg gcggggtgat gttcaaaccg ctgaacccgg tctacatcat
      541 cgtggggggt gcgggatgct cgtaggcgtg gggctaggcc cgggagaccc ggaactcctg
      601 actctgcggg cggtcaggct ccttcgagag gcaaaagccg tgtttgtccc gggcaaactg
      661 gcctacgacc tggtacggcc gtaccgggcc gacgcggtcg tccttaaatt ccctatgacg
      721 aacgatgagg gctacatccg aaggtgtctt gaggagaacg ccgacctgat tgcgcctcac
      781 gccatggatg ggcttgcggt cttcgggatc cttggcgacc cgaacttcta ctcgacattc
      841 tcccggctcg ccggggtgat cgcagagcgc cacccgggag tctcgtttgc gaccgaaccc
      901 ggggtcagct ccatcacggc gtttgcatcg gtggcaaacg tcccggtctc cgggcgggtt
      961 ctggtctcgg acgggaacgg ggaggagtca agggtcatca tgaaggtccg gcggccggcg
     1021 gcgcttgcgg ctgacctgag agaggagggg ttctcggagt ttgtgctggt ggaacggatg
     1081 ttcatgccgg aagagcgggt ctaccgcggg gacgaacttc ccgaggagag cgactacttc
     1141 tcgatcctgt ttgcgaggcg gtctcatgaa taaggtctac atagttggcg cggggcctgg
     1201 agcgcccgac ctgatcaccg tcagggggat ggatctgctc cggaaagcgg acgtcctgat
     1261 atacgcgggc tctcttgtga acccggcgct tgttgaggag tcgggggcgg cgctgaaact
     1321 tgacagccag aatatgaaac ttgatgagat agtcggcgcg atcgaggagc acgtccgcgc
     1381 cgggggactt gttgtccgtc tccattccgg cgatccgtcg ctctacggcg cgatagttga
     1441 gcagatggcc gaacttgagc gccgggggat cgaggccgag atcgtgcccg gggtctcatc
     1501 gctctttgcg gcctcggcgg cgctcaggac gcagtttacc ctccgggacg tctccgagag
     1561 cctggtcatc acccggccgg cgggggcgac gcttgaacgt gacctgatcc cggagttctc
     1621 caggctcggc cagactatgg tggtctttct cgggacggaa cggatggagg aggtcctcgc
     1681 ccgggtcgag tgcccgcccg agaccccggc ggcggtcgtc taccacgcat cctggcctga
     1741 cgagaaggtt gtccggggga cggtggccga tatcgccggg aaggcccgcg ctgccgggat
     1801 cgagcggacg gcgctgataa tcatcggcag ggtggtggac ccggcaacat cagggttcag
     1861 gaggtcggtc ctctactcat gaccgggact gtcgtggtcg ctctcgaccg gttcctgcct
     1921 tccgcccgga ggatcgccga gacgctcggt gccgacctgg tcgcctacac atccggggtc
     1981 ttcgcggatc tcttcggccg atacgaccgg atcgtcgccc tgatgtctgc cgggatagcc
     2041 gtcagggggg tggcgccgct cctggtcgag aagtggcgcg accctgcggt ggtggttgtg
     2101 agcccagacc tccgttacgc cgtcccgatc atcggggggc atcacggcgg aaacgacctt
     2161 gcgcgggaac ttgccgggat cgggatcacg ccggtgatca cgaccgcgac cgagacgcag
     2221 ggccgggact ctgtcgaggg tatcgccgcg cggtcgggct gcgatatcgt gaaccgtgac
     2281 tccaccaggg cggtgaacgc tgcgatgctc gattcggatc tgccgctcta cgccatcagg
     2341 ggccccgcta tcgtcgttgc ggggccggga gtctccatcc tcgtccggaa gggggactac
     2401 gttgttggta ttggttgccg gaagggcgct ccggccgccg ctgtcgccgg agcgctgcgg
     2461 caggcgctgg agcaggccgg gatcgcgcct gaagacgtat ttgtctatgc aaccaccgca
     2521 aagaaacggg gggaggcggg tctgcgtgat gctgtcgcgg aactcggggg caacctggtc
     2581 tatttagacg acgataccct gaacgccgag agagccccat ccccctcacg ggcgtcgctg
     2641 atcgggctgg caggggttgc agaaccggcg gctcttgcga tctcgaagcg aaaagaactg
     2701 gttcttgcca aacagaccta tggatgtgtc accgttgcaa tcgcacgata aaagaggcaa
     2761 gttatacata gtcggcacgg gtcccggcga cctgctgcac ataacccccc gggctctgaa
     2821 agccctggcc gaggccgagt gtgtcatcgg taacgggatc taccttgacc tggtgatgcc
     2881 ggtgatcgcg ggcaaagagg tcatccgtag cagcatgggc cgggaggtcg agcgggcctc
     2941 gcgtgcgctc gaccttgcac gtgaccgcag ggtcgcgatg gtgagcggcg gcgatgccgg
     3001 ggtatacggg atggcgggca tagtcctgga ggtggccgaa agttcgggct cgacggtcga
     3061 gatcgaggtc atccccggga tcacggccgc tctctcggca gcggcacgac tcggttcgcc
     3121 gttgtccggc gactacgtga cgattagcct ctcggatctc ctgacgccgc tggaggagat
     3181 cgagcggagg cttgaccttg cgttccagat gggggtgccg gtcgtgctct acaacccccg
     3241 ttccagggga cggccgcaca acctggagac ggcgatcggg atcgcccgcc ggcacctccc
     3301 ggacgagacg ccggtcgggg tggtcaggaa cgcctaccgg gatggggagg agcggcttat
     3361 cacgacgctc ggggagtttg acgggcacat ctcgtttgtg gatatgcact cgatcgtatt
     3421 tgtcggcgga gaggagacaa ggatctggag gaatgaggct gatgagagag ggatcatcac
     3481 cccgagggga taccaccgga agtacgtata ctgaccttgc ggcaatcacc ccggaggctt
     3541 acgcgatagc aagcgcgagc aggagcctgg cgcgggaacg gctggggaat gccacgcccg
     3601 aggacaggat acggcagcgg tgctcgatcg cggtcggaga ctttgcgatg gccgatctga
     3661 tgcggttctc cggcgacccg gtcgaggccg gggtcgccgc gcttgaacgc cgggcgcctg
     3721 taatcgtcga tatccggatg gtgcaggcag gggtactgag acgggggcat tcaagcgaga
     3781 tcatctgcgc acttgaccac ggggagggga tcgcgatgga gcgcgggatc acccggacgt
     3841 cggcggggtt ccttgccctg cgcgaccgtc tggagggctc gatcgttgtc atagggaacg
     3901 ctccctcggc gcttctcgtc gtctgcggga tgatacggga gggtgtccac ccggcgctgg
     3961 tcgtcgggac gccggtgggg tttgttaacg ctgcggagtc gaaagaggag ctccgggcgc
     4021 ttggtgttat cccctcggtc tcgaaccttg gcacccgcgg cgggacgccg gtagcggttg
     4081 cggcggtaaa cgagatcatc acgatctatg ccgagcgtgg aggtcatgcg ggatccggtc
     4141 actgggtttg agtatccgga tgagtgggtc gcggcctgcc gctctccgga actcctcgac
     4201 gacgtgcagc gtggtcttgc ggttctgaca gcctcggggc tggtcctccg tcgggggttc
     4261 tcaaccggga cgacggccgc ggcggcctgc aaggcagcgg ttctctcgct tgccctcgat
     4321 accgtgaagg aggtggaggt cagactgcca tgcggcatcg ctgtcaggct tccggtggac
     4381 gcctaccgcg gtcaggcgtc gtgctggaag gatgcggggg actacaccgc ggacgtgacc
     4441 gcagggcttg agttcgtcgc gacggcggca ccctctctca atgatggagc gcacttcgtc
     4501 ccgggtgagg gcatagggag tttcgaccgc gacaccccac gccatcgcca ggggacgccg
     4561 gcgatcagcg ccacggcgct tgagtgcatc aggcgctcga tcgacgaggc gatggaggag
     4621 accgggttat ccggggcgac gatcatactc accgttccgc ggggtgctga ggtggcgcag
     4681 aagacgttga acccgaaggt cggggtgcac gccgggatat cggtgctcgg gacgacgggg
     4741 cttgtggaac cctgggacga ccacgtgacg gagggggttc ttgagcgcgt tgcaaacgcc
     4801 gatgcggtgg tgctgacgac cggacggctt gggctgcgat actcccggct cctcttcccg
     4861 gaacgtgagg ccatccttgt cgggaaccat cttgctgaag cgctccaggc tgccgcgggc
     4921 ggtgatccga tcgtctgcgg gttgccgggc ctgatcctga ggttcatgaa cccgcagatc
     4981 cttgagggaa cgggctgtgc gacggtggag gaactgatgg cgacccccgg gggggaggag
     5041 gtgctccggc gcgaacttga ggcgttccgg tcgcggtatc ccggtgtcag ggtcgtgata
     5101 gtcgaccgcg gtggccgggt tataggggag tcgccatgaa gattgtcggt gttggctgcg
     5161 gtcccgggat gctgacagcg gaggctgtga gggttcttaa ggatgcgtcg gttgtctatg
     5221 gctccgcgag ggcgatcgcg cttgcccggg acgccatccc gccggggtgt gaggtgcacg
     5281 ggatcacgga ttaccggagc ctgcgctcgc tcccccgcga tgcggtcgtc ctctcgaccg
     5341 gcgacccgat gcttgcgggg ctcgggccgc tcctggaggg tgatatcgtt cccgggatct
     5401 catcgctgca ggtcgcgttt gcgaggctaa gactcccgct cgcgagggtg gcggttgtcc
     5461 gggctcacgg gaatgaccat gaccacgccg tcgccagcgc cattgaagag atagagaggg
     5521 gccggatcgt cttcatcatt gctgaccctg ccttcgaggt tgaggggctc gcggctctcc
     5581 tcccgccggc agcccgggtc gccgtctgcg aggacctggg ttacccgacg gagcggatcg
     5641 ctaccggaac ggcggcagag ccgccgtcgc cacgcgggga tctnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnccagggc ctgccctggt ttctgggcgc cccggtctcc
     5821 ggtctcctca gcgtcggcag caggcatccc ggccggatcg cccggatctc ctcgatcttg
     5881 cgctcgaaga tctcctctga gacctcaaac ccggtcaccc gccggttgag acctatggca
     5941 gcctttgcgg tcgagaaccc gcccaggaac atatcgcaga ccaggtcgcc ctcgctgctg
     6001 ctgtactgga tcatctttgt gaggagttcg gccgggagtt cgttcttgtt ctttgcccgg
     6061 ccgggtttgt agtcgcggtt gatgcaccag acgtcctcgc ggtcgctgta gttgggcgac
     6121 cggttatccg gcccgcgttc cagagcgccg taacgcgact ctaggttaaa agtcctctct
     6181 ccacccggtt tttcgtagaa gaggatgtgg tagtgggagg agatgtactt ccggctggtg
     6241 tagactccga agttgtagcg ccagatgatg tggttgacct cacgaaggcc ggtcccccgg
     6301 agggcgtgga ggatgtggta gaggtttgtg tagccggaga tgatgtatat agagcccccg
     6361 ggtcttagta tacgttcagc ctcagcgatc catctacggc tgaactcgcc gtagtcggcg
     6421 gcggcgacct cgacgtaacc gtcgatgacg aacctctcgt cgcggttgta gtgacggtgc
     6481 agccggtcgc cgttgatgcc gtagggcggg tcggtgatga tcaggtcgat cgagtcgtcg
     6541 ggtatgtgtg cagcggcgcc gctgatgcag tcaccgttgt agaaggtgtt gccgtagatc
     6601 acctcggacc tcatgcatat ccccggtgta ccgggtttgg ccctccatcg cgataagata
     6661 ctcggatggt gggggagggg gctattgatg ccatgtggag gggttcaccc tccacacaac
     6721 acaagccctc ccctcaccac caccctgcct tacttttgcg ctctctcaac cgttttttgc
     6781 gcagcctcag ggccacaacg gcggcaacga tcgtgacaag tatggcggcc agtagaccca
     6841 ggcccagggc gttgttctcg cggtatatat cgaggaggag atcggcgcca agagcgatca
     6901 ggaggctgga taaaaacgac cccgtgcaga cacagagaaa gacccgtgcc ttctccagcc
     6961 ccagcagtcg gccaaggatc gagccgttca tcgcccctgt cccctgcaag gggaagaaga
     7021 caaagaggat aagcccgatc gtcgagaaac gcctgaccca tggccgtgcc tcaaagaatt
     7081 cccggcctgc cctaaccccg ctctcaatca acccgccgat gagcggaatc ttcagcgcca
     7141 gatcgaagtt ccatactata aagagcgaga caactgcatc catcatgaag atcgcaaacg
     7201 tgacaagcca ccagtggtag cccatcatta tcgcgatggg gatgatcgac tcctttcccg
     7261 caggagggat gaagtaggcg gcaagcagcc ccgagaggtt gaggaaatcc tggtatggca
     7321 ggagcaggta gaggatcgca aaaagtataa tcccgatccc gaaagggagg aggaacttta
     7381 tgggaccggc aatgtagcga ttctcgagga tggtcctcca ccatacccgc tgcgatacac
     7441 ccgttgtatc atccatgatt cgtccctcga tcccgcccgg gaaggctccc cattacatct
     7501 ggaggctccc tcttaataca ttagccgtag atggatccag catattcaag tccgcggaac
     7561 gcaaggatag gtagcatgac ctctcctcct gccacccgat cgccccgcaa cctcccgctc
     7621 atcgaggccg ggagccggga gagcgccctc tgcacaatcg cgaacgtcgc ggccgctctt
     7681 gagacgctgc gagaggtgcg aaaacacgtc cgcggcgacc acaagcgacg gctcgacgat
     7741 gtagcggtga tcctccgcgt aatcgcgttc gacacccagg caacctacgc gatcaccgat
     7801 gacgaggcgc gtgagttcat caggaagtgc cgcccggtgt gagatcgccg ggcagcaccg
     7861 ggtcggtgag gatgcgggca aaccgttccc accggtcagt gccactcccc acatactccc
     7921 ggacgagccg gcaacccccg aagatcgcca ccgggtatcc ccggaactcc tcgatgaacc
     7981 tgagccgggc ctctgccccc tcccggtcaa ggagcagaac gtcctggagg agcctgcacg
     8041 cctcctcccg cgagagcagg ctggcgagat accccctggc ggcgcgggcg ttccccgcaa
     8101 gttccagggc ggcggccgcg ctggcaacgg tatccaggag acctgcgtcc gccgggtcaa
     8161 accccgccag cggaaagaga acctcctcgt agaaccggat gcgttcgcgg tcaggaaaga
     8221 tcatctcctc gccgtagagg gcggcaccct cggcgaccgt cgtgaacggt gatgccagcg
     8281 gaatgaccga gtgctcgatc cagccccggg cacggacgag cgccgcctcc cggatcgccc
     8341 ggatcgtgtg gcggccaggg tagccatggc ggcaggcgtg ggcgagcacc cgatcgatgg
     8401 tgaacggggt ggtggtgctc accccgacca ggcttctccc gcccccctga tagcggctgc
     8461 agccctcacc gctgacagcg gcaacctcaa gatgctctcc tgccggcagc ggcaggtgtt
     8521 ccgctgtccg ccggcggctt tcggcaacag cttccgcaaa gaccgtttcc agccggtcgg
     8581 ccgggacaag gaacaactcc ataaaacccc ggtaacggga ggcaaggtcc cccgtgccgg
     8641 ggagatgtga atctagcgtt gcgtgaagcc ccccgtagta atccggatca tcctccggtg
     8701 ccgcgatgcc aaagagcgat ctaacctcgg catcgaagcc agggcgcttc cccgccagga
     8761 tcgcggcacg ggactcaagc gcccggatca tcccctggag ataggcgcgg cgcctcctcc
     8821 acggatcgtc caggtgcccg ggttcgatcc gatcaagggt cgcaaggagg tctgcggcgt
     8881 aaccggcgac tcgatccggc gggacgctga ccgtgagcgc ctcctctcgc accgcctccg
     8941 gcccgaaata agcctccaca aaattcgggt cgtgttgccc cagccagagc acgcatttga
     9001 cgtagaatcg cgcgacagca tccatatctg cggcgttctc tcccgtgtat gttgaaggct
     9061 ccatccacct ctccaggaat ggtatgggcc ccaccgttac ttaacaccgc cgtgaggctc
     9121 catctccttg cagtgggttt aaccgcccac gcgccaacca caggggtgat gaacgaaccg
     9181 ctccgcaagg ctgtccacct catctttggt cttgctatcg cctgcttcat cctcttctcg
     9241 gagcgcaaac tcgccctcta cgcccttgta atcgcccttt ttctgggttt catcctctcc
     9301 gatgccctct cccggggcta caccatccct ggcgtctcaa agatcatcgc gaccctggag
     9361 cgccgggatg tcctcccggg gaaaggagca ctcttctttg ctctcggtgc gctcttctct
     9421 ctgactttct ttccaaaaga ggtcaccttc atcggcctcg tggtgctcgc gctcctcgac
     9481 agcgtcacca ccctcgtcgg cctcaggttt ggcaggacgc ggatctacaa ccacaaatcc
     9541 atcgaggcca cactcgcagg gtttgcggcg acgaccgccg ccctcctctt cctcataccg
     9601 gcccacgcag ccatcctcac atctgcagcc gcagcgtttg ccgaactcgt aagccccgtg
     9661 gacgacaacc tgatcgtgcc ggccgtcgtc tgcatcgccc tcacggtgct gttttaggga
     9721 cgctcccccc ggcgcccctc tgtctcagga tctccgctgg cgtgggagtg gtggtcgcac
     9781 tccggacagg tggcgaagac accacaggct gagcagtcct cgccggtgca gggctggacg
     9841 cggatcgtga cgtttgtcct cggaaactcc tgccgtatgt cgtcctccag gtgtttcacg
     9901 atcccgtaag cctcttcgag agtcgctgtc cgggggacga tcaggtgaag gtccacgaac
     9961 cggttcggcc ctgaccggcg tgtccggagg cggtggaagt cgatgacatc cgtgcagtgc
    10021 ccgtggatga tctcccggat cctctcctcc tcctcgacgg gaaggctctg atcgaccagg
    10081 tcctggaacg agcggcggat gaggccaaag gccgccctga ggatgatgac ggcgaccgca
    10141 agtgcgatta tagcgtccag caccacaaga ccggtcagcc ggatcaggag aagccctgcc
    10201 acaaccccaa gagaggtgta gacgtctgtg cgcaggtgcc aggcatcgct ctcgagcgcg
    10261 atcgactcgg tcttctttgc cgccgccatc agccttgatg agacaaagag gttcaccacg
    10321 gccgagagca gcatgacggc cataccagac cagagcccct ccatatacag cgtcggttcg
    10381 ccgccgaaga ggcttcgcaa cgcctcgatg atgatcatga cggctgcagc caggatgagg
    10441 atcgcttcga tgagccctga taggctctcg tacttcccgt gcccgaaggt atggcactca
    10501 tcgggaggcc tggccgactg ccgtaccgag aagtaggcga tggccgcggc catcaggtcg
    10561 atgccggagt ggatcgcctc ggatatgatc cctaccgatc cgatcgcaag accgactgca
    10621 agttttgtcg cgacaagcag cgtgtttgag gcaacggaga ggcgtgcggt cttctccttc
    10681 agtatctcat cccgctcttc cgcagcggct gctcccatac cctatgagta tcttcactcc
    10741 agcgggatag ggtttgtgtc ccggtaggag gggccagagt tcttttcctc tcagtcgagc
    10801 gtgaggatga caagcgagtt cgtccccctg ccgccagggg agacgccttc cgtcaggacc
    10861 acgcgttcac cgggcgctac cagtcccgat tccctgatca tatccaggat cgccccatgc
    10921 cggtagtctt tctcgctctt gaccaggaac gggcaaaccc cgcaggagaa cgccaggaac
    10981 ctgaaggtct cagggctccg ggtgaaagcc aggatccatg ccgccggttt gaaacgggat
    11041 atgctgcgtg gcgtcgtccc ggtgcgggtt ggtgtgagga cgtaacggat accgagcgca
    11101 tccagcgcct ggatgacgtt gagcgagacc acatcgttga ctgtgatcct ctcccgttcc
    11161 atcccctgcc ggaagtgctc cagggtggca gacccagccc cgataaccgg gcggcttgcc
    11221 tcggttgaac gggctatctt tgccatcatc gcgaccgtct cgacgggatg gctcccgatg
    11281 gaggtctctt ccgagagcat gacggcatct gtgccgtcca ggatggcgtt tgcgacatcg
    11341 gtgacctctg cccgggttgg ccggatgttc tcggtcatcg acatcagcat ctgggttgcg
    11401 gtgatcaccg gccggcccag gatgttggct ttcaggatga tccgtttctg cactatgggg
    11461 acatcctcga tgggtgtctg gatacccagg tctccacggg cgatcatgac accgtcggcc
    11521 tcctggagga tggcatcgat attcgcgagc gcctccgccc gctcgatctt tgcgatcaca
    11581 tgtatggact tcccgaaccg cgctgcatgc tcccgcgccc tccgtatatc ctccgnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggt ctcctccgag gccgcccgga
    11761 tgttacggat gttctctctg tgctcctcga acgtcccgtg ggcaaggttg agccgggcaa
    11821 cgttcatacc ggcctggatc atccggacaa gcacctcgca cgaccgggaa gcgggcccga
    11881 tggtcgagac tatctttgtc ctgtggtcag ggaggcttat ccgtctcccc ccagaacctg
    11941 gggctgtatg gtatatcatt gagtagtctt cgggcagcgc tgccggatta accttcccca
    12001 gggggatggg ttctggagat gccgtcagga ggtgacacgg gcaccgtggc gccctttatg
    12061 ttcctccacc gggataaccg ggatcttctg gccgctctcg gcactgtact cgcctatgcg
    12121 ccgtttgaca tgctctacca ttccgccgct ggtgatgatg agcgagtcct cccctatcag
    12181 ggctttcagg atgcaccggt cgatgacgcc gtaggtgtag tcagggtaga tccgctcgcg
    12241 gcctgcaaac tcctttgcac gtgtccccat ggcgccgacg tgctccacgg ccccggactc
    12301 tataaaccgc cgcagttcat cgccgcatac cctctcggcg tcgcagaggt agacactgcc
    12361 cacccgctcc gctgtcacgg cgcggccgtg gatgatccgc tcgatcctgt cggtgaactc
    12421 gagcacgctc tttggaggag cgtccccgtc tatgatgccc cggagagcaa gctcgttgta
    12481 gaggtcgagc agggcggggg gctttaacct tgccgccgcc aggagaccgg ggtcggagaa
    12541 gacctcctct ggcgaccccc gtgcaaggat cctccccctc tccatcagta ttgccgagtc
    12601 agcccagcgg taggcgagtt cgacgtcgtg ggtggatatg agcaccgtcc ggccgctgga
    12661 ggcgagttcg tcgaggagtt ccatcacctc ctcggcgctt gcggggtcaa gggtgttcgt
    12721 cggttcgtca aagacaagga cttccggctc catcgccagg atgcccgcga ttgcaacccg
    12781 cttcttctcc ccgccggaga ggtggtgggg cgggcgtctc tcgtatccct ggagaccgac
    12841 gtagccgaga gcgtcccgca ccgccttccg gacggcatcc ggaggaagac ccaggttcag
    12901 cggcccgaag gccacgtccg cctccaccgt cggtgcgatg atctggagat cggggttctg
    12961 gaagacgaac ccgacccggc gccggaggtc gcggaggctc cggttgtcgt aggcgatcgg
    13021 tcggccggcg aaccggatct cccccctgtc cggccggagc ataccgttga acatcaggag
    13081 gagtgttgac ttgccggcgc cgttagggcc gacgatggcg gtctttgacc cggcatcgat
    13141 ccggaggctc acaccggcaa gagcagcggg gccgttcggg taggtgtagg tgagattctc
    13201 ggtctcgatg agcgctttca tagcatccgc agacccccgg ttgtgagcga tacccagagc
    13261 accgccccca ggtatgccag ggtcagcgcg agcgagaggc ccgagactgc ctggttctcc
    13321 tcagggacgg ccagcacccc atcgtagcag cgggcgtcca tcgccataac cagggcttcc
    13381 ccgctctccc acgtccggat gaacagcgct ccggcaagca tcccgagaga gttcaccgat
    13441 tccctgaacc ggccataccc gagccgcatc acctgcgagc ggtagatctc gccggcctca
    13501 tcgatgagta tgaagatgaa ccggtaggtc agcatcgcca gatcgatgag aacggtgggc
    13561 acccggaggc ggtggagcag atcgaagacc tcggtcatcg gtgtcgtgag cgcgatgaag
    13621 tagagcgagc acatcccccc gaagaccctt gcaaggacca ggatggcgag gtttgcgccg
    13681 tccgttgtga ccgcgagcgg gaggccggga aactcgatga tgaccgtccc gctcccggtg
    13741 atgaagagga tgacgacgat gctcataacc gcaaacgaga ccgggatgag gagaagtctg
    13801 gcgtagaacc gtgcaggtat ctttgcaaga agaaggatgg cggcgctcat cgaggccgcg
    13861 accagaaggg gggcgatcgg ggccggcgat gagacgcaga ggagaatgct cgcgaccccc
    13921 aggagcaact tgagccacgg ggctgtctcg cggagttcgt ttccccgtgc gaggtcttcg
    13981 agcatctcgt gcatctactc tcaccggttc aggcggtctt cttgccggcc tgccagtagc
    14041 cgaagacgta gcctatgatc acggcgccga tggcggcctg cagggcaaag aggagcgact
    14101 cgatctcgcc gctcggcggc tcccagaacg gctcaatcca gggctcgtag cccgaggcct
    14161 cgatgagttc tgcggcctcg ccgtcagccc cttcccagga tttatcgccg gcagcgtcct
    14221 gaaacaggaa tacgccggca aatatgatga ttatagcggc gaccgcgatc tccatgccgt
    14281 gcttcatgcg gccgcctccc tcagtctctg gatggtgctc tcgggaagga ggttcaggcg
    14341 gatcaggatc tcgggtttga ggtggactat gtacttaaag accagcgcga tgaccgcacc
    14401 ctcgatgatg gcaagcggca cctgggtgat ggcaaatatc gccgcaaatg ccgtgaacgc
    14461 tccaaggaac ccggtggtgc ccggaaacgc aagcgcaagc tggatggatg tgacgacgta
    14521 tgtgaagaga tccgcaagcg ctgcagcggc aaagaccgtg gcgtaggtgt tcgcgcccgc
    14581 ccgcgtccct gccttgtaga ccagccaggc gacgaccggg ccggcgatcc ccatcgagaa
    14641 tacgtttgcc ccgagggtgg ttatcccgcc gtgcgccagg aagaccgcct ggaagatgag
    14701 gacgataagt ccgagaacgc ccgtgatgca ggggccgaag aggacggccc caagccccgt
    14761 cccggtggga tgggagcagc tcccggtgaa cgaggggagt ttgagcgagg agaggacaaa
    14821 gacgaaggcg cctgcaaccg caaggagcgg cagcgcctcg cgcctctctt ttacaagccg
    14881 gtttagctgg tatactccaa agactatgaa gggcagggag acaaggaacc agatctccca
    14941 ccagggactg ggcaagaatc cttccattat gtgcatctta tcactcctga ccgctctcgc
    15001 agcggtaaaa caaattttat tgatctaaag taaaacattt taatgtatta tggttatttg
    15061 tggtcatatt tatcccctcc ggtattcaac tctccaggat cgttatgcac cggattccac
    15121 gcatcgttct cgcaggaacc cacagcggct gcggtaagac cacgattgca agcgggctga
    15181 tggcggcact cgcggccagg gggctcgctg tccagccctt caaggtgggc ccggacttca
    15241 tcgaccccac gcaccactcg gccatctgcg gcaggccgtc ccgcaacctc gacccattca
    15301 tgatgggtga ggcgggggtg cgtgagacgt ttatcagggg atccgccggc gccgcgatcg
    15361 ctatcatcga gggggtgatg gggctctacg acggtctcga agggacggat atcgcgagca
    15421 ccgcacatgt tgcaaagatc ctggacgccc cggtcatcct ggtggtggat gcggagggcg
    15481 catcacgaag cgtccatgcc atggtcaggg gctacgcggg gttcgaccct gaggtcaggg
    15541 tcgccggggt cataatcaac cggatcggga gcccccgcca ccttgccatg atcgaggaga
    15601 cagagagcct cccgatatac ggggcggtgc cgcggcgaaa cgatctcgcg gtcgagagcc
    15661 ggcacctcgg gcttgcgatg gcggccgaga ccgggatgat ggcgcgatac ggcgcggtgg
    15721 tggaggagac ctgcgatctt gatgctgtca tcggcctggc cgcctcagcc cccccgctca
    15781 ctgacacggg aacggcgatg ccccgccagg agacccgggt gcggatcggt gttgctgagg
    15841 atgcggcgtt ctgcttctac tacgccgaca acctggagag acttgcccgg gcgggggcgg
    15901 atctggtatt cttctcgccg atggccgacc gtctgcctga cgtggacgcc ctctacctcg
    15961 gcggcggcta ccccgaactg cacgcggggg ctctctcctc gtccaggtgc cggggagatc
    16021 tgcgccgggc ggccgatgac gggatgcctg tatacgcgga gtgcggcggg ctcatatacc
    16081 tcagcgaacg gctaaccgcc gacggggccg attacccgat ggcgggaatc ctcccggcaa
    16141 ccgccgagat gaccggccgc atccaggctc tcgggtatgt ggaggccagg gttgccggcg
    16201 gggcgccacc ggtgcttgcg cctggctccc tcttccgggg ccacgagttt cattactcgc
    16261 gcctggagtg cgcccgcgac gcaaggttcg cactggaact cagccgcggc cgcgggatcg
    16321 acgaggggag ggacggtctc gccgccggca gcgcggtcgg gcagtacacc cacgcctact
    16381 tcacggacga gtttgcgcag acgcttgtta aagccgctgc cgggttcagg cggtcttagg
    16441 accccggtgc ctgaagaggc caggaggatg agcctttgag agccggtgaa ctggagagcc
    16501 tgcgtgagcg catcgccgcg tcgcctgacg cgatcctggt gatggtcgtg ctcgtcatct
    16561 tcatggacat gatgatctac ggcctcctga tcccgatctt cccgcagtat gcctcgagcc
    16621 tcagcgtgga ggaatcggtc atcggggtca tattcgggct gtacgctgcc atgcttcttg
    16681 tattctccat accgatgggg ctcctctccg atcggctggg gcgccgaccg ctcatagtcg
    16741 tcgggatgct cctccttgct cttgcaacag ccctctttgg gctctccagc acggttctgc
    16801 acctggccgc cgccaggact gtccagggca tatcggcggc ggcgacctgg tctgcagggc
    16861 ttgcgctcat cgccgataca tgcgatcccg ccaggctcgg tgagaagatg gggatcgcgt
    16921 tgtcgtcggt cgggctcggc acggttcttg ggccggtcgc cggcgggctg ctgtttgagt
    16981 atgccggcta caccgcgacg ttcatactcc cggcgatact ggcggcggtc atcggtcttc
    17041 tcgtccttgc ggtcccggtg cgcacacccc ggcgagagag cccacacctc ctcccgcggg
    17101 gcgcgctccc caacctcgcg gtctgtgcgg cggtgatcgt cgcggtctcc gggacatacg
    17161 gcgtgatcga cccctacctt cccgtccacc tccatgcagc cttctccgcc tcgccgggaa
    17221 tgatcggggg catgtttgcg gttctggcgg ccgcggccat aatcgcccag ccagccgcag
    17281 gccggattta tgaccgctgc ggcgggagcc gctgccttat acccggcggg ctcgtccttg
    17341 ccggggccgc gaccctggcc gccgtagagg cagagaccat cctcataacc gccgtttcgg
    17401 tcttcgtgct gggggtcggg cttggctctg ccctggtgcc ggtgatgccg atcctggcag
    17461 agatataccg cagccagggg tcgcagggcg ccgcctacgg catatacaat actttctact
    17521 ctgcggggct ctctgcgggg ccgtttgcag gggcggtggt cctggcaagg tgggccttac
    17581 cgactctatt cctgctgctg gcagttatcc tcgcggctgc aggagtctta tgctggtcag
    17641 ccatcgggag agccggctgg cgctgaagat acgttctttg cgttcccggc caaaacatga
    17701 ggtatgttcc aggagttctg ccgccgccat acccctgaac tgctgatcgc cctcttcggg
    17761 ctcatattcg tggctgcagc gttcgggggg gttgaaggtg aggcaggagc gttcctcgcc
    17821 tcggcactcg atgtggcgct ctttgtcatc ctggcaatgc tcgtccatct cgcaggaacc
    17881 cggcacccgg cctaccgccg ggctgccatc atctggcttt ttgccgtggt tgccgggctt
    17941 gccatcacca cggcgagtct tggggcggtt gccatccttc cggaggagat cgatcccgac
    18001 ctattcgacc ccgaacttgc cgccacggtt gcccttctct tcctgggcgt tttcgctgcc
    18061 gggcttttga gcctcgtcgg gctctcgcgc cggttccggg tctggcttgc aggctacct
//
LOCUS       DAOSZX010000022        17630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2657_length_17630_cov_21.361309, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000022 DAOSZX010000000
VERSION     DAOSZX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17630
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2657_length_17630_cov_21.361309"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..86)
                     /locus_tag="PK196_03375"
     tRNA            complement(12..86)
                     /locus_tag="PK196_03375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Arg,seq:tct)
     gene            complement(153..542)
                     /locus_tag="PK196_03380"
     CDS             complement(153..542)
                     /locus_tag="PK196_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HON40496.1"
                     /translation="MKYAALTGISPSVIAELKSGKPRTLELNSAHNIITLTGTAPGDC
                     IFMTSITEEDLSPGDPGIMVDLLALNISMKRVEVANPVFYEERERMSARIKVRFRGVT
                     IARDVEGRKWGEPTRVEVVTSACYRAG"
     gene            complement(548..1261)
                     /locus_tag="PK196_03385"
     CDS             complement(548..1261)
                     /locus_tag="PK196_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HON40497.1"
                     /translation="MDDIKVTARAPGGTDETVLIGFPGSGLVGSIALQYLVEQMEFEQ
                     IGAITSKYFPPVALMAKGVISAPVRLYEKDNLLTVVSDVPIHPAICYEVANGIMDWLT
                     RFDIKEVVTIAGIITNEPEKRVFGVATSPELLQRIEDKAIILPMGSLSGVAASILTEC
                     KTRGIPGVGLLGETVNTPDPRAAAATIEVLNQIYDLNLDVEPLLEQAVEIEAAMAQIA
                     EQVQKTEGQPRREQLPMYG"
     gene            1321..1647
                     /locus_tag="PK196_03390"
     CDS             1321..1647
                     /locus_tag="PK196_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HON40498.1"
                     /translation="MQEYQIKRGYTKKLTESMLQCLRDQFGVEPRLTEDGHYIISYGA
                     LLRLEVWAGAGGRTLFVHTESDSSADDETILDTNRRFRNYLQQVTGYTAKERAKKAQQ
                     SVKGKD"
     gene            complement(2254..5022)
                     /gene="leuS"
                     /locus_tag="PK196_03395"
     CDS             complement(2254..5022)
                     /gene="leuS"
                     /locus_tag="PK196_03395"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HON40499.1"
                     /translation="MQNNERECAERWEREHAFEADPAVKDKYYLTVAYPYPSGAMHVG
                     HGRTYIVPDVIARYQRMKGKQVLFPMAFHVTGTPVIGISKRIAKGDEQTIGLYRDIYR
                     VPQDILDRFIDPMEIVRYFSDEYRRVMQKCGLSIDWRRRFITVDPQYSKFIEWQYQHL
                     HEKGHVVKGAHPVKYCPQCENPVGDHDLLEGDKAEIVRFTLVVFLLGDIKIPCATLRP
                     ETIYGVTNLWLRPDLTYVRAKIDGDEWILSREAAAKLALQDHDVQITGEVAGTSLIDR
                     RVSHPLCGEVPILPATFVDPDMGTGIVMSVPAHAPFDYVAIRDLQREGRYTSIQPIPL
                     ISVEGYGDIPAKDAVERAGIQDQNDPDIEKLTQEVYNAEFSRGRVFEKYGGKPVKEAR
                     DEVAALMMERYGSIPMYEFDTRQVICRCGSRVYVKILHDQWFLAYSDPSWKEEVKTVL
                     EGMALVPPEVRAEFERTVDWLKDWACTRRVGLGTKLPWDPTWIIEPLSDSTIYMAYYT
                     IAHHLKGIPAENLTPEVFDYIFLGEGEPATLDRSTLERLRSEFLYWYPYDYRFSAKDL
                     ISNHLTFQVFHHSAIFPPELQPRGMVVFGMGLLNGAKMSSSKGNVFLLEDALSEFGAD
                     TVRMFLVGSAEPWQDFDWRNDLVSSTKKQIERFWNTIQEGKGAEGSCEIDAWLESRLQ
                     RRIESTTAALESFQTRQALQEAFFGVEADLKWYRRRLPEGVTGGEVLRDLCHTWVRLL
                     APFIPFTCEALWRDLGEEGMVSFAPWPEADETKINPEVELAEELLARTVEDIESIKKL
                     IPMEPKSISLYVAPAWKHEIFRMVASSDDRTRGMHTLMQNEEIRRRGREATDAAKQIT
                     KLVLKLPPHLVQQIAELKLDEQEILESSRTFLEHEFGVPVSVQTAGESVHPKASGALP
                     FKPAIVIE"
     gene            complement(5078..6967)
                     /locus_tag="PK196_03400"
     CDS             complement(5078..6967)
                     /locus_tag="PK196_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HON40500.1"
                     /translation="MKIVPDTSVVIDGRITSLIKTGEYKGATIIIPEAVVAELEAQAN
                     QGREIGFSGLTELQELFRMSEEGLIELQFAGERPSLDQVKLSSGGEIDALIRNVAIDH
                     KARFITSDLVQAEVAKAKGLDVLYLRPQIGDFSPLSIDQFFDEETIAITLKERALPVA
                     KIGKLQATRLVTLRDTPMTEHELKVMAQELLERAKRDPDGFIELEKRGITVVQIGSLR
                     ISIARRPFSDGMEITVVRPLVDLSLDDYAMAPEIKQRILGDRRGVLIAGPPGAGKTTL
                     AQSLATFLADHNFIVKTMEAPRDLQVPDHITQYTALEGSMVNTAEALLLVRPDYVIFD
                     EIRKSEDFAVYADMRLAGMGMVGVVHAMEVHDCLRRFCDRVDYSILPQIINTIIFVVR
                     GEIKKIYDLELTIKAPEGMPADAHARPVIVVRNHTRGEPEIEIFRYEGETLVMPLAKK
                     EIVEPVAVAEPPVIVTPAAESRESAARKIAEKDVQREIGRYTNGPVDVRIINDNKAVV
                     YIEDRDVPAAIGKGGKNVSAIVNKLGIGIDIRPRSELEAEKPAEGEIHLSGGLTIRLD
                     KKQLTIVSPEHRGRIVDVFAGKEYLFTATVNEEGEVILARNSTIAQELMRRYNDAETI
                     RLRPV"
     gene            complement(6991..7356)
                     /gene="hisI"
                     /locus_tag="PK196_03405"
     CDS             complement(6991..7356)
                     /gene="hisI"
                     /locus_tag="PK196_03405"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HON40501.1"
                     /translation="MDIDFKNGLIPVIAQDRRTRDVLMLAYANAEALELTRTTGYAHY
                     YSRSRQKIWKKGEESGHIQRVCRILVDCDADALIYEVEQTGAACHTGHASCFFRTIEG
                     EEIAGLVFDPEKVYANKDE"
     gene            complement(7362..8135)
                     /locus_tag="PK196_03410"
     CDS             complement(7362..8135)
                     /locus_tag="PK196_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HON40502.1"
                     /translation="MISAGAIGITLIYASILDLRERRVPHKTWRPALVIAVPVAVWVY
                     VLTFIADWRTAAAYLTLAAVFCGFFYLFQAMNLFGGADAYALIVITACIPFHPFETIS
                     GTSPLGFFPFTVLINAVLINIAAPLAILLKNLIEGNRAPLSCLFLGFPVSGEKIQNEF
                     GFVMEDIEEGEDGRLVRRFIGFTESLRRIIRGERRIYTKDLRQHPDEYQKELTLYRKA
                     GRVWISYGIPFIIPITAGFLTALFVGDIFFVIMKAIAGM"
     gene            8485..9117
                     /locus_tag="PK196_03415"
     CDS             8485..9117
                     /locus_tag="PK196_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40503.1"
                     /translation="MEINDLMAEVLGQSLCEGRFTYEELLHYSAAHSLSGIGVSRGEA
                     SDFFLIISRGEPDGAILVDGKGTLFGDLAALHLTGDETFELYPVAQTVVDALTSRCRV
                     FEKSHLKKNGRLDIPIVGATDRQRVGVLCLTVHDGRAPLAGARVTIRKGKVMLASDVT
                     DTGGRVCFRLLNGRYMCIVSDRIGERRRCIVEFHEPRVEVGIDVGRRPDE"
     gene            9110..11473
                     /locus_tag="PK196_03420"
     CDS             9110..11473
                     /locus_tag="PK196_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HON40504.1"
                     /translation="MSKNDDEERELIAAVRNLLSQPQKLGFGEGQEAIANDPASELFI
                     DTWWGERPEPVPVHVRKDDEIRALIESVARSGRAAERRKTPAEDPIQSLLKRIEGQGA
                     GIVEEPASSYLEVPGEAGTALESPAAPSVVEEISRPEPAFTPAASRRHTHGGIRERIR
                     GWRETDESGSYEETFEGVRSVDELGNLADLILPKSATLSIDELSLNHNDHFYDFVDND
                     RDVSEFDEIFSRSYTPSSFAAAAAEVAIPAEEVPKPRFGVIRKFQMPGFEQKIEDYNP
                     LLHGPLVDLTLRPSPGVEEIELYPVNEPYAFIRVTYDASTHEYTYHVIEPVLTPGERD
                     LFQEIRERLFETLNITCRNLGREEARRVLRDAVNTIIADYGIRLDPVGREKVLYQVER
                     EFLGDGLIDPIMHDKYVEDISCDGVGSPIFVYHTTYESLRTNLAYTDAAELDSFVTKL
                     AQRAGKYISIAEPMLDATMNDGSRIQMTLGSEVTAHGSTFTIRKFRDEPITPTDLIEW
                     HTFSQLGIAFLWLAVENGKSCIFAGGTASGKTTTLNAISLFIPPLAKIVTLEDTRELK
                     LPQPNWIPSVTRDSFSQDGRGEINMYELLRAALRQRPEYILVGEVRGREALTLFQAMS
                     TGHVTYATMHADSVASAVHRLENPPIGVPRNMLSALDLMSIQVQARVGGQRIRRNKQL
                     IEILDVDPRTNELITNEVFRWHPATDEIRYSGKSYILEQIMEDRGWSEERMREELKRR
                     QEVLEWMRIKKIRHYQDVSKILISYFRDPEAVIQRVRADLYGEGGHA"
     gene            11470..12393
                     /locus_tag="PK196_03425"
     CDS             11470..12393
                     /locus_tag="PK196_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HON40505.1"
                     /translation="MNSYERFCFNLLGRRLKDKRADYVKLRNDMVGARMTTPFEAYLA
                     TAYVTSIMVGLVAAVLIGLLTYILRIPDMIVYRGAVPEFLYALNDYKMVLGTIVITII
                     ALLVFGGITYMIFIIYPGTLAGERRRNIDATLPYAVNYVTAMSSAGITPDEVFRSLGQ
                     SQIYGESAVEARYVTRETDFFGKDLLEALRAVAQATPSDRMREFLQGAVASISSGSNL
                     TEYFRTKAHQYTLENHQQQKAFLETLGLVAESYVTAIVAGMLFLIILQSVMTILSGDS
                     NPLFLYIIIYLLVPFGSLMFVILISSITPEV"
     gene            12395..13444
                     /locus_tag="PK196_03430"
     CDS             12395..13444
                     /locus_tag="PK196_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HON40506.1"
                     /translation="MGFKDLFDDLLNRNRPQPVDMPEEAPQPDPVAVRDEEILKRIQQ
                     RRKYQEGFYRFLKHPLRVMIEEPYTALFISVPAALLLFIGGFVSIVTSYGFAAISGTT
                     MLDDVAVFAIIIAIVPFAILDLKESIRVSSVEASLPNFFRDVAGMNDSGMTLPHAIHI
                     VSEGDCGALTPHIRKLDTEMSWGVPFVEAIRRFGKAVSTPLAERSIELIAQASSAGGD
                     VSEVLRAAAHDAYEFFNLKTERKTGMMIYMIIILMSFFVFLFVIAVLSGTFLATMAEA
                     GETVAASGSASAQSFMGSVDIFLYNRVFCHAALIQGFFSGLAAGVMGEGRVVAGLKYS
                     ALMVLIAWVAFRFFL"
     gene            complement(13899..14744)
                     /locus_tag="PK196_03435"
     CDS             complement(13899..14744)
                     /locus_tag="PK196_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON40507.1"
                     /translation="MRQIAIYGKGGIGKSTIASNLSAALHEMGYTVMQVGCDPKRDST
                     RILAGGKFIPAVLEEHREQLRVGKDEYAISLDRIVYRSPDGIYLVEAGGPEPGVGCAG
                     RGVLTALQILKDLDAFSTYHIDVAIYDVLGDVVCGGFSMPIREGFAREIYLICSGGFM
                     SIYAANNIARAVQRLAKRGDTGLAGLICNSNGDEAFERAVIPAFGEKLGTPFFRFVPR
                     SPVIQACELEESPVVVHAPGSAEAEIFRDLARAVMENDSRVIPTPVNDLAELEEMYRQ
                     HLAKI"
     gene            complement(14752..16110)
                     /locus_tag="PK196_03440"
     CDS             complement(14752..16110)
                     /locus_tag="PK196_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HON40508.1"
                     /translation="MEAGSIEEAETRIRPSMESCTDPYLRCAFYGAALTALGVSGSAL
                     LAHSPQGCDYLVNNAFAAQECDYIETMTLCTKLCVDEIVHGGEEALARTIREVKGLPI
                     SCLFVISACGPEIVGDDINAVCEMLQPEMQCRLIPIASAGFRGSQYDGIDIALDSILK
                     RLSSQDAEKVPGSVCLIAPHANANPTWPADLAWVKQVLSRMGVPVVATLAHRTPISEI
                     MNAAAAETSIVLSHDAGQKAAEYLHDAHGVEPICQGIPLPIGMTNTQRWLLALGERFD
                     RLKIAEEIISEGEQMVMAACRQKWPVARFLYRIPAAIVADATVGIPLVRFVTEDMEMT
                     PALVALYTSTRSVRDLLAQELGSLGLSPEVVYGTDVYKTRQRIRDVKPRIVFGSTIER
                     HASEGVPVAYVVEVVRPMRQFRLIDREYFGYTGILNLFEAVQNEWTMAWRSKEKRYSA
                     KW"
     gene            complement(16097..17470)
                     /locus_tag="PK196_03445"
     CDS             complement(16097..17470)
                     /locus_tag="PK196_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HON40509.1"
                     /translation="MLGKVESGEMIPKLQASHAPMCKFFTTYYVISGIRHVVPLVHGP
                     TGCPLYMSDFVRTRECCEIRGIPLEPTACTALDESHVIYGGEGKLLEAVKEAYAKYHP
                     DLIVILSCCCSGIIGDDVESIARIAEEEVGCRVLALRSEGFGGDFRSGYEDAFKVLLE
                     LMEPPEKTMKGVINILGARWGPTPTEYHYDLDEIERLLHEIGIRINAIIAGGCTVEQL
                     RHAREVELNASWCYDWGQKLGDLMQVRFGIPYSRTGQPYGLKATEEWLLGVAEPLGME
                     REAQEVIEKEVRQVQPEVDYLHEALAGKTAIIEITEFPGPIRALSLARMAEEFGAYPI
                     VINVHPYTVKERMPSIKYILETGLNPELILTKGLFELGSFRSSKETEDELEAIVQEYE
                     DPIFFGNSGRFPPCPVVNLSLMNPSYQPHYGYNGIKNLAALVKQALEHSALPRSRLFR
                     GMLYGSG"
BASE COUNT         3554 a         5213 c         4916 g         3947 t
ORIGIN      
        1 aagcggtcag acgggcctgg agggattcga acccccgaca tccgggttag aagcccggcg
       61 ctctatcctg actaagccac agacccacgt ggtagttcta tacccgttgg tttcccggtg
      121 atattaatcc tttgaggggg gaaagttgag tgtcacccgg cccggtagca agccgatgtg
      181 acgacctcga ccctggttgg ctccccccat ttccgcccct ccacgtcgcg ggctatggtc
      241 accccccgga accggacctt gatccttgca gacatcctct ccctctcctc gtaaaagacc
      301 gggttggcaa cctcaacccg cttcatgctt atattgaggg cgaggagatc gaccattatc
      361 cccgggtctc ctggagagag gtcttcctcg gtgatcgagg tcatgaagat gcagtctccc
      421 ggtgcggtcc cggtcagggt tatgatgttg tgagcgctgt tcagttcgag ggttcgtggc
      481 tttccgctct ttagttcagc gattactgat ggtgagattc ctgttaacgc tgcgtatttc
      541 atgcagatca cccgtacatc ggcagctgct ctcttcgagg ctgcccctcc gtcttctgca
      601 cctgctcggc gatctgggcc attgccgcct cgatctcaac cgcctgttcg agcaatggct
      661 cgacatccag gttgaggtcg tagatctgat tcagaacttc aatcgttgcc gcagctgccc
      721 gcgggtccgg cgtgttcacc gtctccccga gcagccctac ccccgggatc ccacgcgtct
      781 tgcactccgt gaggatgctt gctgcaacac ctgataggct ccccatgggg aggatgatcg
      841 ccttatcctc gatacgctgc agtaattcgg ggctggttgc gaccccgaaa actctctttt
      901 ccggttcgtt tgtgatgatc ccggcgatcg tgacgacctc ttttatatca aaccgggtga
      961 gccagtccat gatgccgttt gccacctcgt aacagatcgc tggatgaatg ggaacgtcgg
     1021 agacgactgt taagaggtta tccttctcat acaggcgcac cggggcgctg atgacacctt
     1081 ttgccatcag ggcgacaggc gggaaatatt tgcttgtgat ggccccgatc tgctcaaatt
     1141 ccatctgctc aacgaggtac tggagggcaa tactcccaac gagcccactg ccggggaaac
     1201 caatcagcac agtctcatcg gtgccgccag gcgctctggc tgtaaccttt atatcgtcca
     1261 tgggatcaat cactcgctct tcggttaggt taagatagat ggatatccct taaaaatatt
     1321 atgcaagaat accagatcaa acgcggatac acgaaaaaac ttacagaatc aatgctgcag
     1381 tgtctccgcg atcagttcgg cgtcgaaccc cgtctgacgg aggacgggca ttatatcatt
     1441 tcctacggag cgctcctgcg ccttgaggtc tgggcaggtg ccggagggag gacgctgttt
     1501 gtccataccg agtcagactc atccgcagat gatgagacga tcctggacac aaaccgtcga
     1561 ttccgcaact acctccagca ggtaaccggc tacactgcaa aagagcgggc gaagaaagcc
     1621 cagcagtccg tgaagggaaa ggattagacg ggttatcctc aggtacaggg caccatattc
     1681 tattttgcgg ccagaacggc cattaagagg gggctgtagg aaccaattct ttcgggctgc
     1741 gccccggttt gatgaacttc gccacgggca accgaccccc gcggctctgt attgccgcag
     1801 accgccttta tacctgtgcc tgggggtggc cgggtttttg cgagacgcta ttacctggag
     1861 ggggggcggg tcaatgaacg agagcctccg gatgagacgc gagtgatggg gaggtggcga
     1921 gacatctcct gctatagtgt cattttcacg gaaacctctt tgaatacccc gtttctatcc
     1981 ctggcgcggc ttcaccatgc caatcgccac caactgcctg ctctcccgtc agcgggagtt
     2041 gtcgacgaaa gacttttccc ggaggcgatt tgatattccc tggaaaaggc agtgagttaa
     2101 tatctcagaa ccgttgattt gatggcctcc aggaggcccc ctcggaggct ggcatgggga
     2161 acccggaatt caacactcct ggccggtttt ccccacggtt cagcccataa atggcagcaa
     2221 gacaaaaaac cggcaggagg atgaaaaagg gtcctactct atcactatcg ccggcttaaa
     2281 cggcaacgca cccgaggcct tggggtgcac gctctccccc gcggtctgaa cactcaccgg
     2341 aacgccgaac tcgtgctcca ggaacgtccg tgaactctca aggatctcct gctcatcgag
     2401 ttttaactca gcgatctgct gcacgagatg tggcggcagt ttcagcacga gtttcgttat
     2461 ctgctttgct gcatccgttg cctcacgacc gcgcctgcgg atctcctcgt tctgcatcag
     2521 cgtgtgcata cctctggtcc tgtcgtctga agatgcaacc atccggaaga tctcgtgctt
     2581 ccatgccggt gcgacataga ggctgatcga tttcggttcc atcgggatga gctttttgat
     2641 cgactcgata tcctcgacag tcctggcgag gagttcctcc gcgagttcaa cctctggatt
     2701 tatcttcgtc tcgtcggcct ccggccacgg cgcgaatgag accataccct cctcgccgag
     2761 atccctccat agtgcctcgc atgtgaacgg tatgaagggg gcaagcagcc ggacccatgt
     2821 gtggcagagg tcacgcaata cctcaccgcc ggtcacgccc tccggcaggc ggcggcggta
     2881 ccatttcagg tcggcctcga caccaaagaa cgcctcctgc agagcctgcc gcgtctggaa
     2941 cgactcgagc gctgccgtgg tgctctctat gcggcgctgc agcctgcttt caagccatgc
     3001 atcgatctcg caggagccct cagccccctt tccctcctgg attgtgttcc agaaccgctc
     3061 gatctgtttt ttagtcgacg atacaaggtc gttgcgccag tcaaagtcct gccagggctc
     3121 agcgcttccc acaaggaaca tcctgacggt gtcggcgccg aactccgaga gggcgtcctc
     3181 gagaaggaaa acgttaccct tgctcgagga catcttcgcg ccgttcagga gccccatgcc
     3241 aaagaccacc atgccccggg gctgcagttc cggtgggaag attgcggaat ggtggaagac
     3301 ctggaacgtg agatggtttg agatgaggtc ctttgccgag aaccggtagt cgtaggggta
     3361 ccagtagagg aactcgctcc tgagacgctc aagcgtgctg cgatcgaggg tcgctggctc
     3421 cccctcaccg aggaagatgt aatcgaagac ctccggcgtc aggttctccg ccggtatgcc
     3481 tttcaggtgg tgggcgatcg tgtagtaggc catgtagatc gtcgagtcgg agagcggctc
     3541 gattatccag gtcgggtccc agggcagttt cgtcccgagt ccaacacggc gggtgcaggc
     3601 ccagtccttc agccagtcga ccgtccgctc aaactccgcc ctgacctcgg gcgggacgag
     3661 cgccatccct tccagcacgg tcttcacctc ttctttccag gatgggtcgc tgtaggccag
     3721 gaaccactgg tcgtggagga tcttcacata gaccctgctg ccgcagcggc agatcacctg
     3781 acgggtgtcg aactcataca tcgggatgga gccataacgc tccatcatca gtgcagccac
     3841 ctcatcccgt gcctctttca caggttttcc gccgtatttt tcaaagaccc tcccccggga
     3901 gaattcagcg ttgtagacct cctgggtgag cttctctata tctggatcgt tctggtcctg
     3961 gatacccgcc cgctcaaccg cgtcctttgc cggtatatca ccatacccct cgactgagat
     4021 gagcgggatc ggttgtatgg atgtgtatct cccctctcgc tgcaggtcac ggattgcgac
     4081 gtagtcaaag ggtgcgtgtg ctggaacgct cataacgatc ccggttccca tgtccgggtc
     4141 aacaaacgtc gccggcagga tggggacctc accgcagagc gggtgggaca ccctccggtc
     4201 gatcagcgac gtcccggcta cctcccccgt gatctggaca tcgtgatcct gcagcgccag
     4261 ttttgctgcc gcctcccggc ttaagatcca ctcatcgcca tcaatcttcg cccggacata
     4321 ggtgagatcg ggtctcaacc agaggttcgt cacaccgtag atggtctcgg ggcggagggt
     4381 tgcgcacggg attttaatgt cgcccagcag gaagacaacg agagtaaacc tgacgatctc
     4441 ggccttatcg ccctcgagca ggtcgtggtc tccgacgggg ttctcgcact gggggcagta
     4501 cttgaccggg tgggcaccct ttacaacgtg ccccttctcg tgcaggtgct gatactgcca
     4561 ctctatgaac ttgctgtact gcgggtcgac ggtgatgaac cggcgccgcc agtcgatcga
     4621 gaggccgcac ttctgcatca cccgccgata ctcatccgag aagtagcgga caatctccat
     4681 agggtcgatg aaccgatcga gaatatcctg gggcacccgg taaatgtcac ggtaaagccc
     4741 tatcgtctgt tcatcgcctt ttgcaatcct ctttgagatc cctatcaccg gggtgccggt
     4801 gacgtggaac gccatcggga agaggacctg ttttcccttc atccgctggt agcgggcgat
     4861 gacgtccgga acgatgtagg ttcgcccgtg gccgacgtgc atcgcgccgc tggggtatgg
     4921 gtaggccacc gtcaggtaat acttgtcctt aaccgccgga tcagcctcga acgcatgctc
     4981 ccgctcccac cgctcggcac actctctttc gttgttctgc atatcaagtc cgctcacact
     5041 attcctccag atatctcttc ttcacagtcc cctcatctca caccggccgc agccggatcg
     5101 tctcagcatc gttgtaacgc ctcatcagtt cctgcgcaat cgtgctgttc ctggccagga
     5161 tgacttctcc ttcctcgtta accgtcgccg tgaatagata ctcctttcct gcaaaaacat
     5221 ccacaatcct gcccctgtgc tccggggaga ctattgtaag ttgctttttg tccaggcgga
     5281 tcgtgagacc gccggaaagg tgtatctccc cctccgctgg tttttccgcc tcgagttcac
     5341 tcctcggccg gatgtcaata cctatcccga gtttgttcac gatcgctgat acgttcttac
     5401 cccccttccc gatcgctgcc gggacgtccc tgtcctcgat gtatactaca gctttgttgt
     5461 cgttgatgat cctcacatcc accggaccgt ttgtgtagcg cccgatctcg cgctggacat
     5521 ctttctctgc aatcttccgg gcggcactct ccctggattc tgctgctggt gtcacgatga
     5581 ctggcggttc cgcaacagca acgggctcca cgatctcctt ctttgcgagc ggcatcacca
     5641 gggtttcgcc ctcgtagcgg aatatctcga tctcaggctc tccgcgggtg tggttgcgaa
     5701 cgacgatcac cgggcgtgcg tgcgcgtcag cgggcatgcc ctcaggggcc ttgatagtta
     5761 attcgaggtc atagatcttc ttgatttcgc cccgaacaac gaagatgatg gtgttgatga
     5821 tctgagggag gatgctgtaa tccacccggt cgcagaaccg ccggaggcag tcgtgcacct
     5881 ccatcgcatg caccacgccg accatgccca tcccggcaag ccgcatgtca gcgtagacgg
     5941 caaaatcctc acttttccgg atctcgtcaa atatgacgta atctggccgt acaagcagga
     6001 gggcctcggc agtgttcacc atgctgcctt caagcgccgt gtactgggtg atgtggtcgg
     6061 gcacctgaag gtcgcgtggt gcttccatag tcttgacaat gaagttatgg tcggccagaa
     6121 atgtggcgag gctctgagca agggttgtct ttccagcgcc tggaggtcct gcgatcagga
     6181 cgccgcggcg gtcaccaagg atccgttgct tgatctccgg tgccatagcg taatcgtcga
     6241 gcgagaggtc gacaagcggc ctgaccaccg tgatctccat gccgtctgag aaaggtctcc
     6301 tcgcgatcga gatccggagc gacccgatct ggacgacggt gatccccctt ttttcaagct
     6361 cgatgaaacc gtccggatca cgttttgctc gctccagcag ttcctgcgcc atcactttca
     6421 gttcgtgttc ggtcataggg gtgtcccgga gtgtgacaag cctggttgcc tggagtttac
     6481 ctatctttgc caccggcaga gcccgctctt tgagggtgat ggcgatcgtc tcctcatcga
     6541 agaactggtc gatcgagagc ggcgagaaat cgccgatctg cggtcggaga tagagaacat
     6601 cgagcccttt cgcctttgca acctccgcct gcaccagatc gctcgtgata aaccgggctt
     6661 tatggtcgat cgccacgttc cggatgagtg catcgatctc gccgccgctt gagagtttca
     6721 cctggtcgag gctggggcgc tctccagcaa actggagttc gatgagaccc tcttctgaca
     6781 tcctgaatag ttcctgaagt tcggtcagac cggaaaaacc tatctcccgc ccctgatttg
     6841 cctgggcctc cagttcggcg acgacggctt ccggtatgat tattgttgct cctttatatt
     6901 ccccggtttt tatcagcgag gttatacgtc catctattac gacgctcgta tcgggtacaa
     6961 ttttcataaa aaatcaccat agttatgaag tcattcatcc ttattagcgt ataccttctc
     7021 cggatcgaag accagtccgg cgatctcctc cccctcgatc gtccggaaga agcaggatgc
     7081 gtggccggta tgacacgctg cgccagtctg ttccacctca tagatgaggg cgtccgcatc
     7141 acagtcgacg agtatccggc agacccgctg tatgtggcca ctttcctccc ctttcttcca
     7201 gatcttctgc ctgcttctgc tgtagtagtg cgcgtatccg gttgttctgg taagttccag
     7261 cgcctcagcg tttgcgtagg cgagcatcag aacatcacgt gtccgcctat cctgtgctat
     7321 gacagggatc aaaccgtttt tgaaatctat atccatacct atcacattcc cgcgatcgct
     7381 ttcatgatca caaagaaaat gtctcccacg aacaatgctg tcaggaatcc cgctgtaatc
     7441 gggatgatga acggaatacc gtaagatatc cagaccctgc cggccttcct gtagagggtg
     7501 agttccttct ggtattcatc ggggtgctgt ctgagatctt ttgtgtagat cctgcgctct
     7561 ccccgtatga tgcggcgaag cgactcagta aaaccgatga accgtcgaac cagcctaccg
     7621 tcctcccctt cttcgatatc ttccataaca aaaccaaact cgttctggat cttctctcca
     7681 ctaacaggaa aaccaaggaa taagcacgag agtggagccc ggttcccctc aataaggttc
     7741 ttcagaagta tggcaagggg tgccgcgatg ttgatgagca cagcatttat cagcacggtg
     7801 aaggggaaga accccagggg cgaggtgcca gagatggttt caaacgggtg gaacgggatg
     7861 catgccgtga tgacgatgag ggcatacgcg tctgcccccc caaagaggtt catcgcctgg
     7921 aaaaggtaaa aaaaaccgca gaatactgcg gccagggtaa gatacgctgc cgccgtccgc
     7981 cagtccgcga taaaggtcag cacatatacc cagacagcca ccgggaccgc gatcacgagg
     8041 gcggggcgcc atgttttatg cggcacccgc cgctctctca ggtccaggat cgaggcgtat
     8101 atgagcgtga tcccgattgc acctgctgaa atcataagcg gaaaggctac catgggctga
     8161 agaatcatgg gcaggtagtt tgtttgcgtg ttatctctta tttctggcgg tcttgccctg
     8221 ctgactgttt caatgtgctt tcacggtggc tggagattcg catgcattgc tctattctct
     8281 ccgggcagga gggcataagg ggtgcccatc gccatgaaac ctctctttcg ctctgcagcc
     8341 tgtctattcc ccggaaatct tttttttaat cttatatccg acaatcataa gatattaata
     8401 atagcgtata gatgtggtat attgtgatag gggtgcaaat cccctttaca acatgaaaac
     8461 acagggcatt gccagaagat cctcatggaa ataaacgacc tgatggcaga ggtcctcggc
     8521 cagtcgttgt gcgaggggcg ctttacttat gaggagttgc tgcattacag cgctgctcat
     8581 tctctaagtg gaataggtgt atcgagaggt gaggcgagtg attttttcct catcatctcc
     8641 aggggcgaac cggacggtgc aatccttgtc gatggaaagg ggacactctt tggtgacctg
     8701 gcagcactgc acctgacagg ggatgaaacg ttcgaactct acccggttgc gcagactgtc
     8761 gtcgatgctc tcacgtcgcg gtgcagggtc tttgagaaga gtcacctgaa gaagaacggc
     8821 cgcctcgata ttccgatagt cggggccact gacaggcagc gggttggggt gctctgtctg
     8881 accgtgcatg atggccgcgc acctcttgcg ggggcccgcg tcacaatccg gaaggggaag
     8941 gttatgctcg caagcgatgt gacagacacg gggggacggg tctgtttccg gctgctcaat
     9001 gggcggtata tgtgtattgt atctgaccgg ataggggagc gtagacgctg cattgtggag
     9061 tttcatgaac ccagggtaga ggtaggtatc gatgttggga ggagaccgga tgagtaaaaa
     9121 tgatgacgag gagcgcgagt taatagcggc ggtcagaaac ctcctctccc agccgcagaa
     9181 actgggtttt ggggaggggc aggaggccat agcgaatgat cctgccagcg aactctttat
     9241 cgatacgtgg tggggggaac gcccggaacc ggtgccggtg catgtccgga aggatgatga
     9301 gatcagggct ctcatcgaga gcgtggcaag atctggaaga gcagcagaac ggcgcaaaac
     9361 cccggctgaa gacccgatcc agtcacttct caaacggatc gaggggcagg gggctggtat
     9421 tgttgaggaa ccggcctcct cttatctgga ggtgcccggt gaggccggca ccgcgctgga
     9481 atcgcccgct gcaccgtcgg tggtggagga gataagtcgg cccgagccgg cgttcacgcc
     9541 cgccgcttcg cgccgccaca ctcacggggg catccgcgag cgcataaggg gatggcgtga
     9601 gaccgatgaa tccggttcat atgaagaaac ttttgagggg gtgagatcgg tcgatgaact
     9661 cgggaacctg gcagacctga tcctcccgaa gagcgctacc ctctccatcg acgaactgag
     9721 cctcaaccac aatgatcact tctatgactt tgtcgataac gaccgggatg tatcggagtt
     9781 cgatgagatc ttctcccggt catacacgcc ctcatctttc gccgctgcag ccgcagaggt
     9841 ggcaatccct gccgaggagg ttccaaaacc ccggttcggg gtcatcagga agttccagat
     9901 gcccggcttc gagcagaaga tcgaagacta caaccccctc ctgcacggac ccctcgtcga
     9961 ccttaccttg cgtccctcgc cgggggtgga ggagattgaa ctctacccgg ttaacgaacc
    10021 ctacgctttc atccgcgtga cctatgacgc ctcgacacat gagtacacct atcacgtcat
    10081 agaaccggta ctcacaccag gggagcggga cctcttccag gagatcaggg agcgcctctt
    10141 tgagaccctc aatatcacct gccggaacct cggccgcgag gaggcgcgca gggtgttgcg
    10201 tgatgcggtt aacacgatca tcgctgatta tgggattcgc ctcgatcctg tcggccggga
    10261 gaaagtcctc taccaggtgg agagggagtt ccttggcgat ggtctgatcg atccaatcat
    10321 gcatgacaag tatgtcgagg atatctcctg tgatggtgtt ggcagcccta tattcgtcta
    10381 tcacacgacc tacgagtctc tgaggacaaa tcttgcatac accgatgcag cggagctcga
    10441 ctccttcgtc acgaaacttg cgcagcgggc ggggaagtat atctcgatcg ccgaaccgat
    10501 gctggatgcc actatgaacg atggctcgcg tatccagatg actctcggtt cagaggtgac
    10561 cgctcacggc tcgacgttca ccatccggaa gttccgggat gaaccgatca ctccaacaga
    10621 cctcatagag tggcacacct tctcccagct cgggatcgct ttcctctggc tggcagttga
    10681 gaacggcaaa tcatgcatct ttgccggtgg gacggcttcc gggaagacca cgacgttgaa
    10741 cgccatatca ctctttattc cgccgcttgc aaagattgtc accctcgagg acacccggga
    10801 gctgaaactg ccccagccga actggatccc gagtgtcacc cgtgactcgt tctcgcagga
    10861 tggacggggg gagatcaata tgtatgaact gctccgcgcc gccctccggc agcgcccgga
    10921 gtacatcctg gtcggcgagg tccgcggccg cgaggccctg accctcttcc aggccatgag
    10981 cactggtcat gtaacctacg cgacgatgca cgcagattcg gtggcaagtg cggtccatcg
    11041 tcttgagaac ccgccgatcg gtgtacccag gaacatgctc tctgcgcttg accttatgtc
    11101 catccaggtg caggcccggg tgggcggcca gcggatccgg cgcaacaaac agctcattga
    11161 gatactggac gtcgatcccc ggacgaacga actgatcaca aacgaggtct tccggtggca
    11221 cccggcgact gatgagatcc ggtattccgg caaatcctac atcctggagc agatcatgga
    11281 agaccggggc tggagcgaag agcggatgcg cgaagagctg aaacggcggc aggaagtgct
    11341 tgagtggatg cgcatcaaaa agatcagaca ttaccaggat gttagcaaga tactgatctc
    11401 ctacttcagg gacccggagg cggtcatcca gcgggtgcgc gcggacctgt atggcgaggg
    11461 gggtcatgca tgaacagtta cgaacgtttc tgtttcaacc tcctcggccg cagattaaag
    11521 gataaacggg cggattatgt taagctccgc aacgacatgg tgggggcgcg gatgacgacg
    11581 ccattcgagg cctaccttgc gaccgcctat gtcacatcga tcatggttgg tctcgtagcg
    11641 gccgttctga tcgggcttct gacatacatc cttcgcatcc ctgatatgat agtctaccgt
    11701 ggggcggtcc cggagttcct ctatgctttg aacgactaca agatggtgct tggcaccatc
    11761 gtcatcacga tcatcgctct cctggtgttc ggcgggatca cctacatgat cttcatcatc
    11821 tacccgggta cactggcagg ggagcgccgc cggaacatcg atgccaccct tccttacgcc
    11881 gtgaactacg tcactgcgat gtcctcggcg ggaataacac cagatgaggt cttccggtcg
    11941 ctcggtcaga gtcagatata tggtgagagc gccgtggagg cgcgttacgt cacccgcgag
    12001 accgacttct tcggcaaaga cctccttgag gccctgcggg cggttgcgca ggcgacgccg
    12061 agtgaccgga tgcgggagtt cctccaggga gcggttgcaa gcatctccag cgggagtaac
    12121 ctgacagagt acttccgcac gaaggctcac cagtatacgc tcgagaacca ccagcagcag
    12181 aaggcgttcc tggagacgct cgggctggtt gcggagtcct acgtcaccgc aatcgttgcg
    12241 gggatgctct ttttgatcat cctccagtcg gtcatgacga tcctatcggg cgactcgaac
    12301 ccgctcttcc tgtatataat catctacctg cttgtgccct tcgggagcct gatgtttgtc
    12361 atcctgatca gttcaataac gccggaggtg tgatatggga tttaaagacc tttttgacga
    12421 tcttttgaat cgaaaccggc cgcagccggt tgatatgccg gaggaggcgc ctcagcccga
    12481 tccggtcgcg gtgcgggacg aggagatcct taagaggatc cagcagcggc ggaagtacca
    12541 ggaagggttc taccgcttcc tcaaacatcc gctcagggtg atgatcgagg agccctacac
    12601 cgccctgttt atatccgttc ctgcagctct cctcctcttc atcggtggat ttgtaagcat
    12661 agtgacgtct tacggtttcg cggcaatctc cgggacgacc atgcttgacg atgtcgccgt
    12721 attcgctata atcatcgcca tcgtgccttt tgcaatcctc gatctcaagg aatcgattcg
    12781 cgtatcgagc gtcgaggcct ctctccctaa cttcttccgg gatgtggccg ggatgaacga
    12841 ttccggtatg acgctcccac acgccataca tatagtctcg gaaggcgatt gtggggcact
    12901 cacaccccat atccgcaaac tcgatactga gatgtcctgg ggcgtcccgt ttgtggaggc
    12961 gattcgcagg ttcgggaagg ccgtgagcac cccgcttgcg gagcggagca tcgaactgat
    13021 cgctcaggcg agttccgcgg gcggcgatgt cagcgaggtg cttcgggcgg ctgcgcacga
    13081 tgcctatgag tttttcaacc tgaaaacgga gcgaaagacg gggatgatga tctacatgat
    13141 catcatcctg atgtcgttct tcgtcttctt attcgtcata gcggtgctct caggtacttt
    13201 ccttgccacg atggcagagg ccggggagac tgttgcggcc tcagggtcag cgtccgcgca
    13261 gtcgtttatg ggtagtgtgg atatattcct ctacaaccgc gtcttctgcc atgcggcgct
    13321 tatccagggg ttcttctcgg ggcttgcggc gggtgtcatg ggcgagggac gggtggtcgc
    13381 agggctgaag tactcggcgt tgatggtgct gatagcctgg gttgcgttcc ggttcttcct
    13441 ctgaggcggt catgatgcat cataggtccg gaactggcgg cgtgccacgt atatctatct
    13501 ctgccccatg cgcaaccggg ctttttcata atcgcaccgt ttaccatgtg ttcagcgtat
    13561 ccaactgttt tcaggtgcct ggactctgag cgatcatctg cgcaggtctg ccggcgctct
    13621 gcgtgttcgc accggatgta tggctggttc tgctcgtact tccggacacg tcgggttcga
    13681 gaaacgcacg cgtacttctg aaccgagatg acatgaagta tacaggccac tcacaattta
    13741 gcagcgcata cctcctgcat gccatctacg gtctgatggc cgcttcaacc ccggccatag
    13801 gttcgaagaa tcttccactg ctgcacgcga ttccccgcag gtaccgggat gtaccttaaa
    13861 atagggttat ggagcgggtg cccggtatgg acggaccttc atatctttgc cagatgctgc
    13921 cggtacatct cctcgagctc ggcaagatca tttacgggtg tcgggatcac tcgggaatca
    13981 ttctccatga ccgcacgggc gaggtctctg aagatctccg cttccgccga gccgggcgca
    14041 tgcaccacca cagggctctc ttccagttca caggcctgga tcacagggct ccggggaacg
    14101 aatctgaaga acggtgttcc cagcttctcc ccgaatgctg ggatgaccgc acgctcgaac
    14161 gcctcgtctc cgttgctgtt gcagatgagc cccgccagtc ctgtatcccc ccgctttgcc
    14221 agacgctgca cggcgcgggc aatattgttc gccgcataga tgctcatgaa cccgccggag
    14281 cagatcagat agatctccct ggcaaaaccc tcgcggatgg gcatcgagaa ccccccgcac
    14341 accacatcgc ccagcacatc gtagatcgcc acatcgatgt ggtaggtcga gaacgcatcg
    14401 agatccttca gtatctgcag cgcggtcagc accccccggc cggcgcatcc tacgcccggc
    14461 tccggccctc ccgcttcaac gaggtagatc ccgtcaggcg accggtacac gatgcgatcc
    14521 aggctgatcg catactcgtc cttccccacc cgcagttgct cccggtgctc ttcgagcacc
    14581 gcaggaatga acttccctcc cgcgaggatc cgtgttgagt cccgcttggg atcacacccg
    14641 acctgcataa ccgtataccc catctcgtgg agcgccgctg aaaggttcga ggcgatcgtg
    14701 ctcttcccga taccgccctt tccgtatatc gcaatctgtc tcatgtctcc atcaccactt
    14761 tgcgctgtat ctcttctcct ttgaacgcca ggccattgtc cactcgttct gcacggcctc
    14821 gaagaggttc aggatgccgg tatagccgaa atactcccga tcgatcagcc tgaactggcg
    14881 catcggcctc accacctcga ccacatatgc cacaggaacc ccttcgcttg catgccgctc
    14941 gatcgtgctg ccaaagacga tccgcggctt gacatcacgg atgcgctgtc ttgttttata
    15001 cacatcggtt ccatacacca cctccggcga aagccccaga ctcccaagtt cctgcgccag
    15061 caggtcccgc actgaccgag tggaggtata gagggcaacg agtgccggcg tcatttccat
    15121 atcctcggtg acgaaacgga cgagcgggat acccaccgtc gcgtctgcca cgatcgcggc
    15181 cggtatccgg tacaggaatc gcgccaccgg ccacttctgg cggcaggcgg ccatcaccat
    15241 ctgctcgcct tccgagataa tctcctctgc gatcttcagc ctgtcgaacc gctcccccaa
    15301 ggccagaagc cagcgctgcg tatttgtcat cccgatgggc agtgggatgc cctggcagat
    15361 cggctccacg ccatgggcat catggagata ctccgctgcc ttctgccctg catcatggct
    15421 cagtacaatg gaggtctcgg cagcagcggc attcatgatc tcggagatcg gggtcctgtg
    15481 ggcgagcgtg gcgaccaccg gcacccccat gcgggagagt acctgcttta cccaggccag
    15541 atccgcaggc catgtcgggt tcgcattcgc atgcggtgcg atgaggcaga cagagcccgg
    15601 caccttctct gcatcttgcg atgataaccg cttcaggatc gagtcgagtg caatatcgat
    15661 gccgtcatac tgtgatcccc tgaaaccggc gctggcaatg gggatgagcc ggcactgcat
    15721 ctccggctgg agcatctcgc agaccgcgtt gatatcgtct cccacgatct ccggcccgca
    15781 ggcggagatg acaaagaggc acgagatcgg cagccccttc acctcgcgga tggtgcgggc
    15841 gagcgcctct tctcctccat gaacgatctc atccacgcag agtttggtgc agagggtcat
    15901 cgtctctatg tagtcgcatt cctgcgccgc aaaggcgttg ttgaccaagt aatcgcatcc
    15961 ctgcggggaa tgggcaagga gcgccgaacc ggagacgccc agcgcggtca gtgcagcacc
    16021 gtagaaggcg caacgcagat agggatcggt gcatgactcc atggaggggc ggattcgcgt
    16081 ctccgcttct tcgatgctac ccgcttccat agagcatccc cctgaataaa cgggagcggg
    16141 gcagcgcact gtgctccagt gcctgtttca cgagggcggc gaggttcttg atgccattgt
    16201 agccgtaatg cggctggtat gaagggttca tgagcgaaag gttcaccacc gggcagggtg
    16261 ggaacctccc ggagttgccg aagaatatcg ggtcttcata ctcctgcaca atcgcctcca
    16321 gctcgtcctc tgtctctttc gacgaacgga aacttcccag ttcgaagagc cccttcgtca
    16381 gtatgagctc cgggttcaga ccggtctcga ggatgtattt gatgctgggc atccgttcct
    16441 tgaccgtata cgggtggacg ttgatcacga tcgggtatgc gccaaactcc tctgccattc
    16501 gcgcgagcga gagcgcgcgg atagggccgg ggaactccgt gatctcgatg atcgccgtct
    16561 tcccggcgag cgcctcgtgc aggtagtcga cctccggctg cacctgccgc acctctttct
    16621 cgatgacctc ctgtgcctcc ctctccatgc cgaggggttc cgccacaccg agcagccact
    16681 cctccgttgc tttaaggcca tagggctgcc ccgtcctgct gtacgggatg ccgaaccgca
    16741 cctgcatcag gtcgcccagt ttctgccccc agtcatagca ccacgaggcg ttcagttcga
    16801 cctcgcgtgc atgccggagc tgctcgacgg tgcaaccccc cgcgatgatc gcattgatcc
    16861 ttatcccgat ctcatggagt aggcgttcga tctcatccag gtcatagtgg tactccgtgg
    16921 gcgtggggcc ccatcgggcg ccgaggatat tgatcacccc cttcatcgtc ttctccggcg
    16981 gctccatcag ttcaaggagg accttgaagg catcctcgta tccgctcctg aagtcgccgc
    17041 caaagccttc gcttctgagc gccagcaccc ggcatcccac ctcttcctcc gctatacggg
    17101 cgatgctctc cacatcgtcg ccgatgatgc cggagcagca gcaggagagg ataacaataa
    17161 ggtcgggatg atactttgca tacgcttcct tcaccgcctc cagcagctta ccctctccgc
    17221 catagatcac atggctctca tcgagagcgg tgcaggcggt cggctccagc ggaatgccac
    17281 ggatctcaca gcattcacgt gtccggacaa aatccgacat atagagcgga cacccagtcg
    17341 ggccatgcac aagggggacc acatgccgga tgccgctgat cacataatat gtggtgaaga
    17401 atttgcacat gggtgcgtgg gacgcctgca gtttcgggat catctcccct gattccactt
    17461 tcccgagcaa ctgctccaga ttgccatgaa atgcgatata atctctgtcc atgcgtaaca
    17521 aagagtaact ccttttgcag atgcgattaa agtttccatc ggatcgaacc cttcccgtcc
    17581 cccatcagaa ttgttcactg acagtggaag cgagggtgca tcatttttga
//
LOCUS       DAOSZX010000023        17573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2676_length_17573_cov_19.754310, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000023 DAOSZX010000000
VERSION     DAOSZX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17573
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_17573_cov_19.754310"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="PK196_03450"
     CDS             complement(<1..1853)
                     /locus_tag="PK196_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HON40510.1"
                     /translation="MDYLEEFPVLFIDDELHSDTAEGRASREIVKELKDEDFPVIEAL
                     TARDGLHAFLSHPHVSCIIIDWDLTPEPADGMLTAADLTAIIRERNPKVPIFLNTEKL
                     AISAIPLGVISRIDGYIWKLEDTPAFIAGHIKRAAKNYLSDVLPPFFRGLMDYVEDYR
                     YSWHTPGHMGGVAFLKNAAGRIFYNFFGENTLRADLSVSVPELGSLLEHSGVVGEAER
                     KAAEVFGADRTYFVTGGTSAANRIVWLATVTPGDLVLVDRNCHASVMHAIIMTGAIPV
                     YLMPARNDYGIIGPIRSGEIRPEIIAEKIRRCPLVEDPASHPVRLAAITNSTYDGICY
                     STERIEESLREMVEYIHFDEAWSGYARFHPIYAGRFGMRRSTDPADPTVFATQSTHKV
                     LAAFSQGSMLHVRQGRGAVDHARFNEAFMMLTSTSPQYTIIASLDVATKMMAGHSGKF
                     LVEEALEEAIVFRKKMVAVAEEIRASPLSPENYWWFTVWQPDCIMDQETERGDQVLQE
                     DASCWLLNPDEVWHGFDGIEEGYAMLDPVKVTILTPGIGPGGKMEEWGIPAAVVTRYL
                     RESGIVVEKTGYYSFLVLFTLGITKGKSGTLLAELFRFKALYDGNSPIEEV"
     gene            complement(1927..2775)
                     /locus_tag="PK196_03455"
     CDS             complement(1927..2775)
                     /locus_tag="PK196_03455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON40511.1"
                     /translation="MSRDRFLVRRMGREEVEIAVDWAEQEGWNPGIHDAEAFHAADPE
                     GFFIAELDGEPIGSISVVNYSEAFAFAGLYIVKPEYRNRGYGSRLFAAGMAHAGNRNI
                     GGDGVVAMQEKYRARSAFTLAYRNIRFKGTGGGSEPEGSVDLGQVPFDRLAAYDARHF
                     PAPRPRFLAAWIRQKGTCGRAVLDDNGMITGYGVIRKCRSGYKFGPLFADTPEIAEEI
                     FLTLSAQVPGEAICLDTPEPNAEAVALARRHGMVAVFETARIYTKAIPDLPLAEIFGV
                     TSFEVG"
     gene            3126..4574
                     /locus_tag="PK196_03460"
     CDS             3126..4574
                     /locus_tag="PK196_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HON40512.1"
                     /translation="MKSEGQTPPAVCAGFDIHYPCYINPGFRTGIAKGLHDQKEGYFD
                     PYLKEDLGVVIDRSFQPATEILIDLLEAGFACALSISGTVVELLEAWYPDMLDLLSRA
                     AKHRNVELLGETYYHSVAGQFRNPGEFEAEILMHRDLMKDYFSSVPTTFAHTDYPITP
                     ATAEVLRDNEIRAAIIEGDSGPALDSDPNHVYTYNGLPILITHCNLADDIAVRFSWRD
                     WDRWPLTADRYAEWISVSPGECITLFLDYRVFGDTIGAETGILEFLRALPAALSAKWV
                     DSIHPSDAVTRFSPRCDIGEMVDADNQQRTILQQSALQALEDAGALVVDKKAWRRLLD
                     TENLRRMAMRSTPCGKPYHPTSQQAVHDHFAAFMRVLSDMEERSAPASRSPRAVLSLR
                     CVPPEKAFHFSSQERPAGYAAHSLQEFVNILEFAPDEVVNYHLDRGDFTRWIEFVIGD
                     TTLAKKISGISDRNELRSTIQKRIDSLWKRIR"
     gene            4556..5728
                     /locus_tag="PK196_03465"
     CDS             4556..5728
                     /locus_tag="PK196_03465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HON40513.1"
                     /translation="METYKIAFFCWESLHSRFKVGGLAPAATHLAEHLAARGHDVHFF
                     TRGDGEDAAINGVRYHYCLPYGDNIVDYCRTMSNMLLDAFRAHDTPPFDILHFHDWHM
                     VEAMHVLRDRNTVLSYHSTEYGRNGGNFGGWWEFREISGKEWYAGYIAKAVTTVSNST
                     KTEVMWLYGVPEWKVTVIPNGIDPDAYRIQVDPGEVKKRYGIHPLAPVVLFVGRLTYQ
                     KGPDLLVQAIPAILARRWDVQFLFAGTGDMRDSLEKMATGLPVQFLGYVADEEHLQIL
                     NACDLVVIPSRNEPFGLVLAEAWSAERCVVATDVGGLAENIENFKDGIKVPVRPESIA
                     WGVCLLINDPAHMQRLGRAGRRKVMERFRWSVVTDRMLDVYRKVVAASRDDGVREG"
     gene            5725..7665
                     /gene="glgB"
                     /locus_tag="PK196_03470"
     CDS             5725..7665
                     /gene="glgB"
                     /locus_tag="PK196_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HON40514.1"
                     /translation="MKSVQQRLPDITKTGSLITDYDVYLFRQGTHTHLYEKLGSHIAI
                     SSGVKGTFFAVWAPNAEEVSVIGDFNGWVAGKNLLRARSDGSGIWEGFIPGVGHRDLY
                     KYYIRSRYNNYSVEKGDPFAHYWEVPPKTASIIWDISYSWHDRAWMRRREEYNNPTAP
                     ISTYEVHAGSWRQPHDGVRRCMSYQELAVELASYMLDAGFTHVEFLPIMEHPLYASWG
                     YQTLGYFAPTSRYGTPQDFMHLVDHLHKRGIGVILDWVPSHFPSDGYGLSYFDGTHLY
                     EHALPAQRYHPEWKSYVFNLGRNEVRSFLISSAVFWLDRYHADGIRVDAVSSMLYLDY
                     ARRPGEWTPNIYGGRENLDAIGFLRKLNDTVHANYPDTLTIAEEATAWAGVTAPGATG
                     GLGFDLKWNMGWMHDTLDYFALDPIYRKYRHDLLTFSICYAFSERYILPLSHDEVVHE
                     KSALIGKMPGDEWQKRANLRLLFGYMFTHPGKKLLFMGGEFGQWSEWNHETGLEWHLL
                     QYAPHQGILRWVTDLNRLYRRKAALHECDADPSGFEWVDFSDAEKSIISYLRRGRSVD
                     DIVLVACNFTPVPRYGYRIGVPFGGFWREVLNSDAVEYGGSGVGNLGGVEAEQVPAHG
                     RAWSLPLTLPPLAVVIFTPEGA"
     gene            7671..10073
                     /locus_tag="PK196_03475"
     CDS             7671..10073
                     /locus_tag="PK196_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HON40515.1"
                     /translation="MKKRAVCIHGHFYQPPRENPWLGEVGMQRSAAPYHDWNERVTAE
                     CYAPNTAARILDADGLIEEIVNNYARISFTAAPTLLSWLARHRPAVYEAILAADHESQ
                     ERYAGHGSAIACCYNHIIMPLATRRDKRRQVVWGVRDFAARFGRMPEGMWLPETAVDL
                     ESLDLMAEAGILFTILAPHQAARVREMGTATWTEVTGETLDTTMPYVCRLPSGRRIAI
                     FFYNAGISQEVAFGDLLSNGERFAERLVEAFSPGGERSELVHIATDGETYGHHRKFGD
                     MALAYCLRSLESHQNVSLTVYGAYLAANPPTHEVTIQEGTSWSCPHGIERWREDCGCS
                     SGAHPAWSQAWRGPLREAIVMVRDTITPRLDEALAEILDDPLTARDDAVSLVLGTWSD
                     EAVEAFIARHTTGNLSAEERQRLLALFEIERQANLMQTSCGWFFDDIAEPGSVQVMRH
                     AKRAIDLTRRVLGLDLEPAFVETLQRARGNDPGHATGAEVYDARIRPRVFDLPRIAAG
                     VALYALFGLEHPAATSGMHAIQVEWPYRLNAGDGRRILGRVHLRSRAAGEEGAYDTAA
                     LFDGIDRIVLGVNEPGGDAAIREALAGPDPAGAIPAGFSRLYTAADLSDEERWAIARE
                     MIPGIEEAVCGVYLDGSTLIRTLKDLGVPLPGAAAHLVAYAGSRCLASIFEEGCPDPG
                     RFLAIAKEVEALNVAPDIPALAAAAGRRIEGSIRAAAARPDDPAPLEEIPRIVGCARL
                     LELPLDLWESQNICIRLRGHYTEMCGRAGRGDGGAERWVEAYRRAAVSLGVRALQGAR
                     RG"
     gene            10070..11566
                     /gene="malQ"
                     /locus_tag="PK196_03480"
     CDS             10070..11566
                     /gene="malQ"
                     /locus_tag="PK196_03480"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HON40516.1"
                     /translation="MNRRGSGVLLHITSLPSAYGIGDLGPAAQRFLDILAGAGQRYWQ
                     ILPLNPTFPAFGNSPYQSNSAFAGNTWLISPEQMVADGFLDAADIADPPWFPVDRVDY
                     PRVIDYKNKLFDRGFERFRERGRDYRYEEFSVDNASWLDDYALFVALKEHFAGRAWGE
                     WPVAVRDREQEPLENCAAKLAERILREKFLQYVFDRQWKRFKDCCRARHVQVIGDIPF
                     YVTYDSADTWSNPGLFKLDEQKRPTAVAGVPPDLFSETGQLWGNPVYRWEAHREERFS
                     WWESRIERALALLDRLRLDHFRGFVQHWEVPAGAATAQNGRWVDAPGRELLTHLVRSR
                     PCLPIIAEDLGYITPDVREMMALFGFPGMRVLLFGFDGDIAENLHAPHNIGRNCVTYT
                     GTHDNNTVRGWFEHEITDRQKDLLFRYIGGSVSADQVHRILVRLAMLSPAETVIIPMQ
                     DILGLGREARMNRPATAKGNWEWRLRPEQMREEAMQEFAGMAEVYGRS"
     gene            11679..13925
                     /gene="glgP"
                     /locus_tag="PK196_03485"
     CDS             11679..13925
                     /gene="glgP"
                     /locus_tag="PK196_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HON40517.1"
                     /translation="MEDSISDQFDHVPERISGLVDLAYNLWWSWNQEARILFKQLNHQ
                     AWRTSAHNPIRMLREIPVEFLNRAANNPAYCHRYDIIMHRFREYMNTAGTWFPENYAD
                     RPPLTLAYFSAEYGLHHSLPIYAGGLGFLAGDHLKEASDLGIPLVAVGFLYSQGYVCQ
                     QINPDGWQEDITQPLDHDAAPVTRVLDAEGNDLIVRVPNIDPPIYVAVWKVQVGRTPL
                     YLLDTDIPCNDPENRGISARLYADGLEQRLRQEIVLGIGGRKVLHTLGIEYSAVHLNE
                     GHPAFALLERIRERVEGGMAFEEALEEVRATTIFTTHTPVPAGHDVFPIDLIERYFAS
                     YYTALGIDRTRFLHLGANPEQPDSGFNMTALALRLSAHHNGVSRANGAVASDMWRSLW
                     HDGPGRDEAPADYVTNGVHLPTWLNTRMQGLYNRYIGPTAPGWLAEHDNPLVWELIDE
                     IPDAELWNLHLWLKAKLINRLRERERLRWTMREVRVTNPGNGGAFLDPSVLTIGFARR
                     FSTYKRAYLIFEDPDRLKRIVNNPWCPVQIIFAGKAHPADTEGKRMLQRIYQHTQDPG
                     FGGRVVFIEDYNNHVARYLVQGADVWLNNPLPPMEASGTSGMKAALNGVLNLSILDGW
                     WLEGYNGRNGWAFGGEAADCDSRDSIDAAAIYDLLEREVVPLYYDRSIDDVPHGWVRM
                     MKESIRSNAPTFSSRRMVKEYVGRYYPLLLKAAAGTPCAVSLIKDSQAQAWRDLTEKA
                     GVNDLFSH"
     gene            14359..15549
                     /locus_tag="PK196_03490"
     CDS             14359..15549
                     /locus_tag="PK196_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HON40518.1"
                     /translation="MLVATNASAYTNDPLWVLTINDKYDIDVNTVDISRDGSYIAAGT
                     ENNYVYLIDKSGQCLWKYSAKGAIYEVAISEDGQYVSAGGFDGNLYYFEKSGEMAWES
                     PGGQIYELTMSDNGEYLAISRSNGNLIGIRRPCTIEYYNKEGLKIWDYSTNDWISSLD
                     ISPDGSYVAAGCHDNNVYFFNREGDLLWSFTTTDAVWSVSISADGSYVAAGGDDQKVH
                     LISKSGQEIWRSPMYVYSDYYQSNRGTQVLDVEIIRDGPMVLAGGRGHNDLYYLYNAT
                     GYCMHATCKEDVYGVRDIEIDDNGRYFVTYSPTTGNDNIRVFDTNGRNLWSFKTKNYQ
                     NCIAISGDGCYVVVGSNDGNIYFFSNTVQSGQTAPTFPVIGIISIILAAGILRKKDLS
                     RDKR"
     gene            15871..16362
                     /locus_tag="PK196_03495"
     CDS             15871..16362
                     /locus_tag="PK196_03495"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HON40519.1"
                     /translation="MTGREQIAIVRHVPYAVLKKRIKHHHGLPEVMPRLLFIRLRYTG
                     VSVVNAADAVGVSGQTGYNWQERWNAAGFDGLVPRYGGGHPSKLTDEQKAELLELLRE
                     HDHWTTVEAQCLIQSHFGVAYSLDQVRRILKSFGMHFAKPYPRDHRRPADAKTLLKKN
                     SRG"
     gene            16359..16901
                     /locus_tag="PK196_03500"
     CDS             16359..16901
                     /locus_tag="PK196_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HON40520.1"
                     /translation="MSKHAVLGFLDECSPQTTANTRRMWSFDHPPLVKNTTKIRANTF
                     AILAVNGTSIITFRERSKQEDIQEVLKEYRRANPNKRLVIVLDNFSSQHAVLVRQYAA
                     GNNIHLVYLPPYSPDLNPIEQIWRAIKREVSATFVRDYEHMAATIARAFEKLAMKRSY
                     WEKWVKTFLSPRYASKLLSK"
BASE COUNT         3822 a         5035 c         5147 g         3569 t
ORIGIN      
        1 aagacctcct cgatcgggct gttgccgtcg taaagcgctt tgaaccggaa tagttccgca
       61 agcagcgtcc ctgacttgcc cttcgttata ccgagcgtga agaggacaag aaacgagtaa
      121 taccccgtct tctcgacgac gatcccgctc tcccggaggt acctcgtgac gacggccgcc
      181 gggatccccc actcctccat cttcccgccc ggcccgatcc cgggggtgag tatggtcacc
      241 ttgacagggt cgagcatggc gtaaccctcc tcgatcccgt caaacccgtg ccagacctca
      301 tcggggttca ggagccagca gctggcgtcc tcctggagca cctgatcacc tctctccgtc
      361 tcctggtcca tgatgcagtc aggctgccag acggtgaacc accagtagtt ctcaggggat
      421 agagggcttg cccggatctc ttcggccacc gccaccatct tctttcggaa gactatggct
      481 tcctcgagcg cctcctcaac caggaacttc cccgagtgtc cggccatcat ctttgtggca
      541 acgtccaggg atgctatgat cgtgtactgg ggcgacgtgg aggtgagcat catgaacgcc
      601 tcgttgaacc gggcgtggtc aacagcgccc cgcccctgcc ggacgtggag catcgagccc
      661 tgcgagaagg cggccagaac cttgtgggtg gactgggtgg caaagaccgt cgggtcggca
      721 ggatctgtgc ttctgcgcat cccgaatctg ccggcgtaga tggggtggaa ccgggcatag
      781 cccgaccacg cctcatcaaa gtgaatgtac tcgaccatct cccgcagact ctcctcgatc
      841 cgctccgtgc tgtagcagat cccatcgtag gtggagtttg tgatcgcggc cagtcgaacc
      901 gggtgcgatg cgggatcctc aacgaggggg catctcctga tcttctcagc gatgatctcc
      961 ggacgaatct cgccgctcct gatgggaccg atgatcccgt agtcgttccg ggccggcatt
     1021 aggtagacag ggatggcgcc ggtcatgatg atcgcgtgca taaccgacgc atggcaattc
     1081 cggtccacaa ggacgaggtc tccgggtgtg acggttgcaa gccatacgat cctgttcgcg
     1141 gccgacgtgc cccctgtgac aaagtaagtc cggtcggctc caaagacctc cgcagcctta
     1201 cgttcggcct ccccgacaac tccggagtgt tcaaggagcg acccgagttc cgggaccgag
     1261 accgagaggt cggcccggag ggtgttctcc ccgaagaagt tgtagaagat tcggcctgct
     1321 gcgtttttca ggaacgcgac cccgcccatg tggcccgggg tgtgccagga gtagcggtaa
     1381 tcttcaacat agtccatcaa tccccggaag aacggcggga ggacgtcgga gaggtagttc
     1441 tttgccgcac gcttgatatg cccggcgatg aacgcaggtg tatcctcaag tttccagatg
     1501 tagccgtcga tcctggatat gacgccgagc ggtatggcgg aaatcgccag tttctcagtg
     1561 ttcaagaata tcgggacctt cgggttacgc tcccttatga tggcggtcag gtctgcagcg
     1621 gtgagcatgc cgtcggcagg ttcaggggtg aggtcccagt cgatgattat acaacttaca
     1681 tgggggtgcg ataggaatgc atgaagaccg tcgcgggcgg taagcgcttc gatgaccggg
     1741 aaatcctcat ctttcagttc ttttactatc tcccgcgaag ccctgccttc cgcggtatcc
     1801 gagtgcagtt cgtcatcgat gaagagaact ggaaactctt ccaggtagtc catacagtaa
     1861 acacttcctg gagggtactc gggagaggta tgcttagaat gttgtggtcg gggcgggtat
     1921 ggatcgtcag ccgacctcaa acgaggtcac gccgaagatc tcggcaagtg gcaggtcagg
     1981 gattgccttc gtgtagatcc gggccgtctc aaagacggcg accattccgt gcctgcgggc
     2041 aagcgccacg gcttcggcgt ttggttcagg cgtatcgagg cagatcgcct cgcccggcac
     2101 ctgcgcagag agggtgagga agatctcctc tgcaatttcg ggcgtgtcag caaaaagcgg
     2161 cccgaactta tagccgctac ggcattttcg gatgacgccg tatcccgtga tcattccgtt
     2221 gtcatcgaga acagcccggc cgcaggttcc tttctgccgg atccaggccg caaggaagcg
     2281 ggggcgcggc gcaggaaagt ggcgggcgtc gtacgcggca agccggtcaa acggcacctg
     2341 accgaggtcg accgagcctt cgggttcgct gccgccgcca gtgcccttga acctgatgtt
     2401 tctgtatgca agtgtgaacg ctgaccgcgc acggtatttc tcctgcatcg caacgacacc
     2461 atcgccaccg atgttgcgat tccctgcatg agccatcccg gcagcaaaca acctgctccc
     2521 gtagccccgg ttccggtact ccggcttcac gatatacagg ccggcgaagg caaacgcctc
     2581 gctgtagttg acgaccgaga tcgatccgat gggttcacca tcgagttccg cgatgaagaa
     2641 cccctccgga tcggcggcgt ggaaggcctc tgcatcgtga attccaggat tccatccctc
     2701 ctgctccgcc cagtcaacgg caatctcaac ctcctcccgc cccatcctcc taacaagaaa
     2761 tctatcgcgg ctcacactgc tcccccctct gcgttgtgcg gtttttccat ttgattctat
     2821 cctgaaagag gataaaggag cgggggcaga aggctgtttc gatcgtttct ggatgtgacg
     2881 tcagatgcat caaccaatgc ttcccgggtt tgatagacct ctccatggat ggatatgtct
     2941 ccatgtgaga cgcagagaga gggggagagg gtgatttggg ggagtgtaaa accatgagtg
     3001 aacgttggtt tgatgtggcc atatccgccg aatcctccgg gcctccaggg atcggggagg
     3061 ggcggaggat tggcgacatt cgtatcaacg acatttatat gtacggaaag ggccatctat
     3121 ctccaatgaa aagcgagggg caaacaccac cagcagtctg cgcgggattt gatatccatt
     3181 acccctgtta tatcaacccg gggtttcgaa ctggtattgc aaagggttta catgaccaaa
     3241 aagagggtta ttttgaccca tacctgaagg aagacctggg tgtggtcatc gaccgctcct
     3301 tccagccggc aacagagatc ctgatcgacc tccttgaggc ggggttcgca tgcgctctca
     3361 gcatctccgg caccgtcgtg gaactcctgg aagcgtggta cccggatatg ctcgatctcc
     3421 tctctcgtgc ggcaaagcac aggaacgtcg aactccttgg agagacctac taccacagcg
     3481 tcgccggcca gttcaggaat cccggagagt tcgaggccga gatcctgatg caccgcgacc
     3541 tgatgaagga ttacttctca tctgtgccga ccaccttcgc tcacaccgac taccccatca
     3601 caccggccac cgcggaggtg ctcagggata acgaaatccg ggcagccatc atcgagggtg
     3661 acagcggtcc tgccctcgat agtgacccaa accatgtcta cacctacaac ggccttccca
     3721 tcctcataac ccactgcaat cttgccgacg atatcgccgt caggttctcc tggcgtgatt
     3781 gggacagatg gccgctcacg gccgaccgtt acgcggagtg gatctcagtc tcgccgggcg
     3841 agtgcatcac gctcttcctg gactaccgcg tattcggcga caccatcgga gccgagacgg
     3901 gcatcctgga gttcctgcgt gctcttcccg cagccctctc cgcaaagtgg gtggatagta
     3961 tacacccatc agatgccgtc accaggttct cgccccgctg cgatatcggg gagatggtcg
     4021 acgccgataa ccagcagcga accatcctgc agcagtccgc ccttcaggcg ctcgaggatg
     4081 cgggagccct tgttgtggac aaaaaggcct ggcgccgcct ccttgacacc gaaaacctgc
     4141 gcaggatggc gatgcgatcg accccctgcg gaaaaccgta tcacccaacc agccagcagg
     4201 ctgtccacga ccatttcgcc gccttcatgc gtgtcctctc ggacatggag gagcggtcag
     4261 ctcccgccag ccgctcaccc cgggcggtcc tctcgctccg ctgcgtcccg ccggagaagg
     4321 ctttccactt ctcgtcgcaa gagcggccag ccggttacgc cgctcacagt ctgcaggagt
     4381 tcgtaaatat cctggagttc gccccagacg aggtcgtcaa ctaccacctg gatcgcggag
     4441 atttcacccg ctggatcgag ttcgtcatcg gcgatacaac cctggcaaag aagatctcgg
     4501 gcatatccga ccgcaacgaa ctccggtcaa cgatccagaa gaggatagac agtctatgga
     4561 aacgtataag atagcattct tctgctggga gtcgctccac tccaggttca aggttggcgg
     4621 gcttgcacca gcggcaaccc accttgccga acacctggcg gcaagggggc acgacgtcca
     4681 cttcttcaca cggggcgacg gggaggatgc agccatcaac ggtgtccggt accattactg
     4741 cctgccatac ggcgacaaca tcgtcgacta ctgcaggacg atgagcaaca tgcttctcga
     4801 cgcctttcgg gctcatgaca caccaccgtt tgacatcctc catttccacg actggcacat
     4861 ggttgaggcg atgcacgtcc tgcgcgaccg caacaccgtt ctctcctacc actccaccga
     4921 gtacggccgc aacggcggaa acttcggtgg ctggtgggag ttccgggaga tatcaggcaa
     4981 agaatggtat gccggctaca tcgcaaaagc ggtcacaacg gtatcaaact cgacaaagac
     5041 cgaggttatg tggctgtatg gcgttccgga atggaaagtc accgtcatcc cgaatgggat
     5101 cgaccccgac gcctaccgca tccaggtcga cccgggcgag gttaagaaac gttatggcat
     5161 ccatcccctt gcacccgtcg tcctctttgt cggccgcctc acctaccaga aggggcccga
     5221 cctcctggtt caggccatac ccgcgatcct cgccagacgc tgggacgtcc agttcctctt
     5281 cgcaggtacc ggcgacatgc gcgacagtct ggagaagatg gctaccggcc ttcccgttca
     5341 gttcctgggc tacgtcgccg acgaagagca cctccagatc ctgaacgcct gcgaccttgt
     5401 agtcatacca agcagaaacg agccttttgg actcgtcctt gcagaagcct ggagcgctga
     5461 gcgttgcgtc gttgcgacag acgtcggggg gctcgccgag aacatagaga atttcaagga
     5521 cggcatcaag gtccccgtcc gtccggaatc gatagcctgg ggagtatgtc tcctcatcaa
     5581 cgaccctgcc cacatgcagc gactcgggcg ggcggggcgg cggaaggtga tggagaggtt
     5641 ccggtggagc gtcgtaaccg accggatgct cgatgtctac cggaaggttg ttgcagcaag
     5701 ccgcgacgac ggcgtcaggg agggatgaaa agcgtgcaac agaggttgcc cgatatcaca
     5761 aagacgggga gtctcataac cgattacgac gtctacctct ttcggcaggg gacgcacaca
     5821 cacctctacg aaaaactggg gtcgcacatc gctatctcca gcggcgttaa agggacattc
     5881 tttgcggtct gggcgccaaa cgccgaggag gtctcggtca taggcgactt caacggctgg
     5941 gtggccggga aaaacctgct aagggcgcgg agcgatgggt caggcatctg ggagggtttt
     6001 atcccgggtg tcggccacag ggatctctac aagtactaca tcaggagccg ctacaacaac
     6061 tactcggtgg agaaaggcga ccccttcgcc cactactggg aggttccgcc gaagaccgct
     6121 tccataatct gggacatctc ctactcctgg cacgatcggg cgtggatgcg gcgccgggaa
     6181 gagtataaca accccacagc tccgatctcc acctatgagg tccacgccgg gtcgtggcgg
     6241 cagccgcatg atggggtgag acggtgcatg agttaccagg aactcgcagt agaactcgca
     6301 agttacatgc tcgatgcagg tttcacccat gttgagttcc tcccgatcat ggagcacccg
     6361 ctttacgcct cctggggtta ccagactctc ggctacttcg ccccgacgag caggtacggc
     6421 accccgcagg acttcatgca tctcgtcgac cacctgcaca agcgcgggat cggggtgatc
     6481 ctggactggg tgccgtccca ctttccctcc gacggctatg gactctctta ctttgacggc
     6541 acccaccttt acgagcacgc cctccccgcc cagcgctacc acccggagtg gaagagttac
     6601 gtgtttaacc tggggcgaaa cgaggttcga tcgtttctta tcagcagtgc cgtattctgg
     6661 ctcgaccgct accacgccga cggtatcagg gtggacgcgg tctcctcgat gctctacctg
     6721 gactacgccc gtcgtcccgg cgagtggacg ccaaacatct acggcggacg ggagaacctg
     6781 gacgcgatag gattcctccg gaagttaaac gacaccgttc atgcaaacta ccctgatacg
     6841 ctcaccatag ccgaggaggc taccgcctgg gcgggggtta ccgcaccggg cgctactgga
     6901 gggctcgggt tcgacctgaa atggaacatg ggctggatgc atgacacgct cgactatttt
     6961 gcgctcgacc cgatctaccg gaagtaccgg cacgacctcc tgacgttcag catatgctac
     7021 gccttctccg agcgctacat cctccccctc tcgcacgacg aggtcgtcca cgagaagagc
     7081 gcgctcatcg gaaagatgcc tggcgatgag tggcagaaaa gagcgaacct ccgtctcctc
     7141 ttcgggtata tgttcacgca cccggggaag aaactcctct tcatgggcgg agagttcggg
     7201 cagtggtcgg agtggaacca cgagaccggg cttgagtggc acctcctcca gtatgcgccg
     7261 caccagggga tcctccgctg ggtcaccgac ttaaaccgcc tgtaccgtcg caaagccgcc
     7321 ctccatgagt gtgatgccga cccttcaggg tttgagtggg ttgacttctc ggatgctgag
     7381 aagagcatca taagttacct gcggcgcggt cggtcggtcg atgatattgt ccttgtcgcc
     7441 tgcaacttca ccccggtacc acgttacggc taccggatag gcgtcccctt cggcgggttc
     7501 tggcgagagg tgctcaacag cgatgcggtt gagtacggcg ggagcggtgt ggggaacctg
     7561 ggcggggtgg aagcggaaca ggttccggcg cacggccggg cgtggtcgct cccgctcacg
     7621 ctgccccctc ttgcggtcgt catcttcacc cccgaggggg cgtaaaccgg atgaagaagc
     7681 gtgccgtctg catccacggc catttctacc agccaccgcg ggagaacccc tggctcgggg
     7741 aggtcggcat gcagcgctcc gccgccccct accatgactg gaacgagcgg gtgaccgcag
     7801 agtgttacgc cccgaacacc gccgcacgca tactggatgc cgacgggctg atagaggaga
     7861 tcgttaacaa ctacgcgcgg atcagtttca cggccgcacc gacgctcttg tcctggctcg
     7921 cccgccaccg cccggcggtc tacgaggcaa tccttgcagc cgaccatgag agccaggagc
     7981 gctacgccgg ccacgggtcg gcgattgcat gctgctacaa ccacatcatc atgccgctgg
     8041 cgacccggcg tgataagcgg agacaggtcg tctggggcgt ccgcgacttt gcggcaaggt
     8101 tcggccggat gcccgagggg atgtggcttc cggagaccgc ggtcgatctc gagtcgctcg
     8161 acctgatggc ggaggccggg atcctcttca ccatccttgc cccccaccag gcggcgaggg
     8221 tgcgggagat ggggacggca acctggacag aggtcactgg tgagaccctg gatacgacga
     8281 tgccatatgt ttgccgcctc ccctcgggcc gaaggatcgc gatcttcttc tacaacgccg
     8341 ggatctcaca ggaagtcgcg ttcggtgacc ttctctcaaa cggggagcgg tttgccgaaa
     8401 ggctggtcga ggcgttctcg cccgggggag agcgctcaga acttgtgcat atcgcgaccg
     8461 atggagagac ttacggccac caccgcaaat tcggggatat ggcgctcgct tactgcctcc
     8521 gatcgctcga atcgcaccag aacgtcagcc tcacggtcta tggcgcctac ctggccgcca
     8581 acccccccac ccatgaggtc accatccagg aggggacgtc ctggagctgc ccccacggca
     8641 tcgagcgctg gcgggaggac tgcggttgca gcagcggcgc gcaccctgcg tggtcacagg
     8701 cctggcgcgg cccgctccgg gaggcgatcg tcatggtccg ggacaccatc acaccccgcc
     8761 tggatgaggc gctcgctgag atcctcgatg acccgctgac ggcccgggac gatgccgtca
     8821 gcctcgtcct gggcacctgg tcagatgagg ctgtggaggc attcatcgcc aggcacacca
     8881 ccggcaacct ctctgcggaa gagaggcaac ggctcctggc gctcttcgag attgagaggc
     8941 aggcgaacct tatgcagaca agctgcggct ggttcttcga tgatattgca gaaccgggga
     9001 gcgtccaggt catgcgccac gcaaaacggg caattgatct cacccggcgg gttctcggcc
     9061 tcgatctcga accggcgttc gtcgagaccc tccaacgggc caggggcaac gaccccgggc
     9121 acgcaaccgg tgctgaggtc tacgacgccc gtatcaggcc gcgggttttc gatctgcccc
     9181 ggatcgcggc cggggtcgcc ctctacgccc tctttggcct tgaacaccca gcggccacct
     9241 ccgggatgca tgcaatccag gtggagtggc cataccgcct gaacgccggt gatgggcgac
     9301 gtatcctggg gagggttcac ctgcggtccc gcgctgcggg agaagagggg gcctatgaca
     9361 cggccgcact ctttgacggg atcgatcgca tcgtcctcgg ggtgaacgaa cccggcggcg
     9421 atgcggcgat ccgggaggcg ctcgccggcc cggatccggc cggggcgata ccggcgggtt
     9481 tcagccggct ctataccgca gcagacctct ccgacgagga gaggtgggcg atagcccgcg
     9541 agatgatacc ggggatcgag gaggcggtct gcggggtcta cctggatgga tcaaccctga
     9601 tccggacgct caaagacctc ggggtcccgt tacccggggc cgcagcccac ctcgtggcgt
     9661 atgccgggag caggtgcctg gcctcgatct tcgaggaggg gtgccctgac ccaggccgat
     9721 ttctggcgat cgcaaaggag gtggaggccc tgaacgttgc gcccgatata cccgccctgg
     9781 cagcggcagc ggggaggagg atcgaagggt ctatccgcgc ggcggcggcc agacccgatg
     9841 accccgcccc ccttgaagag attccgcgca tcgtcggttg cgcgaggctg cttgaactgc
     9901 ctctcgacct ctgggagagc cagaacatct gtatcaggct gcgcgggcac tacacggaga
     9961 tgtgcgggcg tgccggacgt ggagatggcg gtgcagaacg gtgggtggag gcctacagga
    10021 gggcggcggt atccctgggc gtgcgtgctc ttcagggggc gagacgggga tgaaccggcg
    10081 aggaagcggg gttctgctcc atataacgtc tctcccctcg gcatacggga tcggcgacct
    10141 gggtcccgcg gcacagcggt ttctggatat cctcgccggt gccggacagc gctactggca
    10201 gatcctcccc ctcaacccga ctttcccggc gttcggcaac tcaccctacc agagcaactc
    10261 ggcgtttgcc gggaacacct ggcttatcag cccggagcag atggttgcag acggttttct
    10321 cgacgccgct gatatcgcgg acccgccctg gttcccggtg gaccgggtcg actacccccg
    10381 ggtgatcgac tacaagaaca aactctttga ccgggggttt gagcggttca gggaaagggg
    10441 ccgcgactac cgttacgagg agttctcggt tgataacgcc tcatggctcg atgattatgc
    10501 cctctttgtg gccctgaaag agcactttgc cgggcgggcg tggggagaat ggccggtcgc
    10561 cgtccgggac agggagcagg agccgctgga gaactgcgcc gcgaaacttg ccgagcggat
    10621 cttaagggag aagtttctcc agtatgtttt tgacagacag tggaagaggt tcaaagactg
    10681 ctgccgtgcc cgccatgtcc aggtcatcgg ggacatcccg ttctacgtca cctacgacag
    10741 cgccgatacg tggtcaaacc cgggactctt caaactcgac gagcagaaga ggccgactgc
    10801 cgtcgcgggt gtaccaccgg atctcttcag cgagaccggg caactatggg gaaaccctgt
    10861 ctaccgctgg gaggcgcaca gggaagagcg gttttcgtgg tgggagagcc ggatcgagcg
    10921 cgccctcgct cttctcgaca ggctgcggct cgatcatttc cgggggttcg tccagcactg
    10981 ggaggtcccg gccggtgcgg cgacggcgca gaacggccgc tgggtcgatg cgccggggcg
    11041 tgaactcctg acgcaccttg tccggagcag gccgtgcctc ccgatcatcg ccgaggacct
    11101 cggttacatc acccccgatg tccgcgagat gatggcactg tttgggtttc cggggatgag
    11161 ggtcctgctg tttgggtttg acggcgatat agcagagaac ctccacgcac cccacaacat
    11221 cggccgaaac tgcgtcacat acacgggcac acacgacaac aacaccgtcc gtgggtggtt
    11281 cgagcacgag atcaccgatc gccagaaaga cctcctcttc cgctacatag gagggtcggt
    11341 gagcgccgac caggtgcaca ggatcctcgt ccgcctcgcg atgctctcac ccgcagagac
    11401 ggtcatcatc ccgatgcagg atatactggg tctcgggcgg gaggcacgga tgaaccggcc
    11461 ggccacagca aaaggaaact gggaatggcg gctccgcccg gagcagatga gggaagaggc
    11521 catgcaggag tttgcgggga tggcagaggt ctatgggaga agttgaaaag accacaaatg
    11581 actcaataat ttcgatatca tctagatatg gagaagatat caaaaggaat ataataactg
    11641 tgagaaaaac tacaccagca atcagaggac agggttccat ggaagattcc atcagcgatc
    11701 agttcgatca tgtcccggaa cggatctccg gacttgtcga cctggcatac aacctctggt
    11761 ggagctggaa ccaggaagcc cggatactct tcaaacagct gaaccatcag gcgtggagga
    11821 caagcgccca caacccgatc cggatgcttc gtgagatccc ggttgagttc ctgaaccggg
    11881 cggcaaataa tccagcttac tgccaccgct acgacatcat catgcaccgg ttcagggagt
    11941 atatgaacac agccggaaca tggttcccag agaactacgc cgaccgcccc cctttgacgc
    12001 tcgcctactt ctcggcggag tacggactcc atcattcgtt acccatctat gcaggcggac
    12061 tcgggttcct tgccggcgac cacttgaagg aggcaagcga tctcgggata ccgctggttg
    12121 ccgtcgggtt tctctactcc cagggctacg tatgccagca gatcaacccg gatggctggc
    12181 aggaggatat cacccagccg ctcgaccacg atgctgcgcc ggtcacccgc gttctcgatg
    12241 ccgagggcaa tgaccttata gtccgggttc ccaatatcga tccgcccata tacgtcgccg
    12301 tctggaaggt tcaggtcggc aggacaccgc tttaccttct ggatacagac ataccctgca
    12361 acgatccaga gaaccgcggc atatcagcca ggctttacgc tgacggcctg gagcaacgcc
    12421 tccgccagga gattgtgctc ggcatcggcg ggaggaaggt gctccacacc ctcggtatcg
    12481 agtattcagc ggtgcacctg aacgaagggc acccggcctt tgcgctgctt gagcggatca
    12541 gggaacgtgt tgaggggggg atggcgtttg aggaggcact cgaagaggtg cgggcgacca
    12601 ccatcttcac cacgcatacc ccggtcccgg ccggccacga tgtcttcccc atcgacctga
    12661 tcgagagata ctttgcgtcc tattacactg cgctcgggat cgaccgaacc cggttcctgc
    12721 acctgggggc caaccccgag cagccagact ccggtttcaa catgaccgcg ctcgcgctcc
    12781 gtctgtcggc gcaccacaac ggcgtctcgc gggcaaacgg ggccgtcgcc tccgacatgt
    12841 ggagatctct ctggcacgac gggccaggaa gagatgaggc gccagcagac tacgttacaa
    12901 acggtgtaca tcttccaacc tggctgaaca cccgtatgca ggggctctac aaccgctaca
    12961 tcggccccac cgcacccggg tggctggcgg agcacgacaa cccgctggtc tgggaactga
    13021 tagacgagat ccccgacgct gaactctgga acctccacct ctggctgaag gcaaaactta
    13081 tcaaccggct ccgggagcgg gagcggctcc ggtggacgat gcgagaggtc agggtgacga
    13141 accctggaaa cggaggtgcg tttctggacc cgtcggtcct gacgatcggg tttgccagac
    13201 ggttctcgac ctacaaacgg gcatacctca tattcgagga ccccgaccgg ctcaaacgca
    13261 tcgtgaacaa cccctggtgc cctgtccaga tcatcttcgc cggcaaggct cacccggccg
    13321 acaccgaagg taagcggatg ctccagcgta tctaccagca tacgcaggac cccggatttg
    13381 gggggcgggt tgtattcata gaggattata acaatcatgt ggcccgctac ctggtgcagg
    13441 gcgccgatgt ctggttgaac aacccgctgc cgcccatgga ggcaagcggg acaagcggta
    13501 tgaaggcagc gctcaacggc gtgctgaatc tctcaatcct tgacggctgg tggctggagg
    13561 gctacaacgg caggaacggc tgggcgtttg gaggggaggc tgctgactgc gactcaaggg
    13621 acagcatcga tgctgcggcg atatacgacc ttcttgagag ggaggttgtc ccgctctact
    13681 acgaccgctc catcgatgat gtcccgcacg gatgggtcag gatgatgaaa gagtcgatcc
    13741 ggagcaatgc cccgacgttt tcgtcacggc ggatggttaa agagtatgtc ggccgctact
    13801 accctctcct cctgaaggcg gccgcaggga cgccatgtgc agtgagcctg ataaaagact
    13861 ctcaggctca ggcgtggagg gatctgacag agaaggcggg agtaaacgac ctcttctccc
    13921 actgattcac aattatcacg gaaaaccgac tgaaaaaact acatgatgcc cacaaggcca
    13981 caggcatacc aggtgaaaat atctcgaggg gccattttca tacgaaaaaa gtgggcccgt
    14041 cgagattcga actcgagacc tccgccgtgt gaaggcgacg tcataaagcc ggccgtttct
    14101 ggtttagtgg tctttaatcg ggtttctgat attccaagaa ccctgcgtac tgatgagaat
    14161 acacctcgaa gaaggggggg attgatatac tttatgatcc ctgcaaaggg catgaggaag
    14221 gattaaacaa attatgcagc tctgcaaaga tgtcgcacca gttaattcgg aagctttatt
    14281 tactgctccg tatctctaaa aattatgcgt atcgagtcct acatctcacg aatatttctt
    14341 tttattgcaa tatcatgctt gctggtagca acaaacgctt cggcatatac taatgaccct
    14401 ttgtgggttt taactataaa tgataagtac gacatcgatg ttaacacggt ggatatttcg
    14461 agggatggtt cgtacattgc tgcaggtacc gaaaataatt atgtctactt aattgacaag
    14521 agtggacaat gtctgtggaa atactctgca aaaggggcta tttatgaggt ggctatatct
    14581 gaggatggcc agtacgtctc tgcgggtggt ttcgatggaa atctatacta tttcgaaaag
    14641 agtggggaaa tggcttggga gtctccagga gggcaaatat atgaactcac tatgtcggat
    14701 aatggagaat accttgcaat ttcaaggtcg aatggtaact taattggcat tcgtcgcccg
    14761 tgtactattg aatattataa taaagagggg ctaaagatat gggactattc aacgaacgac
    14821 tggattagta gcctagacat tagtcccgat ggatcgtacg tggctgctgg atgtcacgac
    14881 aacaatgttt acttttttaa ccgtgaaggg gatctgctct ggagttttac gacaactgat
    14941 gctgtctgga gtgtgtccat ttctgcagac gggtcttatg ttgctgcagg aggagatgac
    15001 cagaaagttc acctaataag taaatcaggt caagaaattt ggcggagtcc aatgtacgtc
    15061 tattcggatt attatcaaag caataggggt acccaggtat tagacgttga gattatccga
    15121 gacggcccaa tggttttagc tggcggaagg gggcacaacg acctatacta tctatacaat
    15181 gctactggtt actgtatgca tgcaacttgt aaggaggacg tttatggtgt tcgggatatc
    15241 gaaatagatg ataatgggcg gtattttgtt acttatagtc ctacaactgg aaatgataat
    15301 attcgggttt ttgataccaa tggaaggaac ctttggagtt ttaagacaaa aaattatcag
    15361 aattgtatag ccatatccgg agatggttgt tatgttgtgg tgggaagcaa tgatggcaat
    15421 atttatttct tttccaacac cgtccagtcg ggccagactg ctccaacctt ccccgtgatt
    15481 ggtattatct caattatcct tgctgcaggt attctaagaa aaaaggattt gagcagagat
    15541 aaaagatagg tgatgtagtg gcacgtaaaa gcaattacga tggcctcagg ttactgttag
    15601 agagtttaga tcagagtcga atatcaatgg cattttcaga tattgagaga gtcattggaa
    15661 gaaaactccc acaaacggca tatgctcgaa atcagtggtg ggaaaataat gatgatgggc
    15721 atacccaagc aaaagcgtgg ctaaatgcag gatggcatgt atcgcatctt attttgggaa
    15781 aacaggtcac gtttacgaag aattctgatg agtaattcat attatagttc tcggcgctac
    15841 ccttttgaac cttgacgtac ctgagttttc atgaccgggc gtgaacagat cgcgatcgtg
    15901 agacacgttc cctatgccgt tttgaagaaa cggatcaagc accaccacgg attgcccgaa
    15961 gtgatgccgc gccttctgtt tatccggttg cggtatacgg gggtgagtgt tgtcaacgcc
    16021 gcggatgccg ttggggtgtc cgggcagacg ggctacaact ggcaggagcg ctggaatgcc
    16081 gcagggtttg acgggctggt tccgcgctat ggcggtggcc atccctcaaa actcacggac
    16141 gagcagaaag ccgaattgct tgagctgttg cgggagcacg atcactggac aaccgttgaa
    16201 gcgcagtgtc tgatccaatc tcacttcggc gtcgcttata gtctggatca ggtccggcgg
    16261 atcctgaagt cgtttggcat gcactttgca aaaccctacc cgcgggatca ccgcagacct
    16321 gcagatgcaa agacgctcct caaaaaaaac tcccgcggat gagcaagcac gcggtacttg
    16381 ggttcctgga tgagtgttct ccacagacaa ccgccaatac ccggcggatg tggtcgttcg
    16441 accacccgcc gctcgtgaag aacaccacga agatccgggc caacaccttc gctattcttg
    16501 ccgtcaacgg cacctccatc atcaccttcc gggaacgctc caaacaggaa gacatccagg
    16561 aggtgctgaa agaatatcgg cgggcaaatc caaacaaacg gctcgtcatc gtcctcgaca
    16621 acttcagctc acagcatgcg gttctcgtca ggcagtacgc cgctggcaac aacatccatc
    16681 ttgtgtatct tccgccatac tccccggatc tcaatcctat cgaacagatc tggcgagcga
    16741 tcaagcgaga ggtctccgcg acattcgtca gggactatga gcatatggct gccacgattg
    16801 cacgggcatt tgagaaactg gcgatgaaga gaagttactg ggagaagtgg gtgaagacat
    16861 tcctgagtcc gaggtatgct tcaaagctgt tgagcaagta actatacttg ccggttcctt
    16921 aaaacaattt aaaaatcaca aacgatattc aaccccctcc cggtcaatcc cgatctgctt
    16981 gagggtctcc aagagcgtgt cggttgggaa atcctgccca tgcatctgga tgacaatccg
    17041 gcgcttcacc gctgaatttt ttccttcaac agcgcccccc tgacgcctct ggaaaataga
    17101 gtggcgagca gggtcatctg actttccgca gtaagagttc cgggtcgact ttttctcaca
    17161 atcctcatga aatcagtcac caatgcaagg gcgtacgact tttataccgc cgatgaagca
    17221 taaaattaaa ccgcaggttg cataatctcg ccataattaa ttaattcagg agttgattat
    17281 cttactgcgg aaggtggggg tcatgaatga cttctctact agatgactta ttttcgtggt
    17341 ttctctcccc aaataaagag gattggtgat gttctgcctg cagaatagat gagaaagata
    17401 ggagaagacg gagagaatag aagagaatat ctggagaaat gaggagagtt ctcttctctt
    17461 ttccagagat aataggagaa tactccttga gattgtgtgt tacgggcaca tacgctgggg
    17521 tagcgtaacg aaagttctgt aaaattgaat tggccctgga tccaacctag att
//
LOCUS       DAOSZX010000024        17060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2791_length_17060_cov_21.009291, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000024 DAOSZX010000000
VERSION     DAOSZX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17060
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_17060_cov_21.009291"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK196_03505"
     CDS             <1..187
                     /locus_tag="PK196_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40521.1"
                     /translation="DVSIAGRKGGSAFIPGGKAGVFPLHPLHPRKLKDSPVPGSEHSN
                     CALPQALLVTHPALNSR"
     gene            complement(393..806)
                     /locus_tag="PK196_03510"
     CDS             complement(393..806)
                     /locus_tag="PK196_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON40522.1"
                     /translation="MAPMARQITFRQIERPRGRGAEENLEWLFNSLGVGEGRDVDQVA
                     RRILAALLSYQSAGEGVSVERISRDLNISSSRVNHHIRNLVDAGVVYRHRKRIYLRGN
                     SLQSLVQELRKDALRVLEDLEAAAAEIDRSFGLDK"
     gene            941..1213
                     /gene="groES"
                     /locus_tag="PK196_03515"
     CDS             941..1213
                     /gene="groES"
                     /locus_tag="PK196_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HON40523.1"
                     /translation="MKIVPIGERVLIKPIKKEEVTKSGIYIPESARETRKEGTVVAAG
                     RRDDGSDLPLKPGDHVLYGGYSSDTFEIDSETYVFVPFKDILAKIE"
     gene            1226..2839
                     /gene="groL"
                     /locus_tag="PK196_03520"
     CDS             1226..2839
                     /gene="groL"
                     /locus_tag="PK196_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106636.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HON40524.1"
                     /translation="MASSKQLMFDEDARKAILVGVNKVADTVKITLGPRGRYVVIDRP
                     TNPIVTNDGVTIAKEISLHDKFENIGAKLVKEVAQNTQDKTGDGTTTATLLTQAILTE
                     GLKNISAGANPNDVRRGIEAAVAAAVEYIRSTSVPVRERNRILQVATISANNDEEIGR
                     LIADAMDKVGYSGLITVEDAKSLETSLDVVRGMQFEQGYISPYMITDPEKMVCEYENP
                     YILITDREITSLKQMLPILEIAAQDGKPLLIIAEDVNGEAQAALILNIIRGSLKACAV
                     KAPGFGDERKELLEDIAVLTGATVISEDKGMKLENVSRQVFGSAHTVRVDGEKTLIVG
                     GKGDRKALEDRMRLIESQINITDSEWKKDELKKRLGNLGGGVAVIKVGAATETELKEK
                     KMRIDDALNATKAAVEEGVVAGGGVTLFRAIKNLDALQFDDDRKIGVSIVRRALEEPL
                     RQIAENAGVEGAEVVATVKRSDDPAFGYNAKTETYENLMENGVIDPAKVVRLGLQNAA
                     SIAGLILSTEVAIAEFDDEKDTKTATIII"
     gene            complement(2910..3335)
                     /locus_tag="PK196_03525"
     CDS             complement(2910..3335)
                     /locus_tag="PK196_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HON40525.1"
                     /translation="MVDTFRIPPLLLAVLGITLACACLGSDPDREKPGSKVIHPAEAA
                     IMIEDMENDPGFVIIDVRTADEFAGGHIPGAINIDAATILEHLKSLNPDGTYVIYCRR
                     GTRSAGVHKLMNEAGFREVYEIEGGISAWQAARLPVAGG"
     gene            complement(3546..5372)
                     /gene="asnB"
                     /locus_tag="PK196_03530"
     CDS             complement(3546..5372)
                     /gene="asnB"
                     /locus_tag="PK196_03530"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HON40526.1"
                     /translation="MCGIAGQFVLNGGEADPSLTAAMAEKLAHRGPDGEGSLCSGPVG
                     LAHRRLAIIDLSDEGRQPMANEDGSIWIVFNGEIYNYRELRDELLAAGHSFLSATDTE
                     VILHAYEEWGRGCLQRFNGMWAFAIWDGRRRELFCARDRLGVKPFYYTVVGGSFLFAS
                     EIKALRAHPDAGKRPNDLMLLAFLAWGVADHTPATMYEGIFQLPPAHTLLVTEKGVGK
                     PERYWDFEMNPNPRGAGVDDEAAARDLLNLLTDAVRLRLRSDVPVGTCLSGGIDSSTV
                     TVLINHLLRSESPESVGDRQKTFSVCFEDGRFDESRYIDTVVAATGVENSRTTPGTDG
                     LWEDIERLLYMQDEPFASLSIYAQYCVMRLAREEVKVVLDGQGADEQLGGYIAYLVPY
                     IRTLLGHGEILAALREVIGILRLHRSFFWWALRQVVVRSQRRDLLQGSPPEVLRYAGT
                     LDEVLKREITVSNLPLLLHWEDRNSMAFSIEARVPFLDYRVVEYLASLPLDQKIRGGV
                     TKYVLRRAIRGLVPDPVRCRSDKMGFVTPEEVWMRGELAPRVLELFSSPEFSGRPYWD
                     AERVLRNYRKFLAGKSAYSTEFWRIACAELWLRGFEEGSAVI"
     gene            complement(5395..5844)
                     /locus_tag="PK196_03535"
     CDS             complement(5395..5844)
                     /locus_tag="PK196_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HON40527.1"
                     /translation="MDFEKILTRIMERGPDAIAEELLQEIEREYGRVPLIFERMAERP
                     EILISHLLYKGAVSETSQLEPKVIELISLAVGAALKCSHCVEYHMQTAMAKGATRAEI
                     LEVILIAGMLANSAVLADAYRVVDVSSTSCPTCDLNGVGLNKTCSDK"
     gene            complement(5850..6791)
                     /locus_tag="PK196_03540"
     CDS             complement(5850..6791)
                     /locus_tag="PK196_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HON40528.1"
                     /translation="MTTLVPVTYKGGVYRHDEIMDLIDDLGGYVVQKHVMAQDVVLQS
                     FVPREDINLIRAVAKPLAGQVTEAPLVGTEIAVVSPSLEIHHLPHTSCDIAEYLRRSG
                     AKTNMVGLARGFGKRIANLNDEERDVINEHDLAVYMLGNFEECIQHKFPVLRRGIHVP
                     IVVTGAPDRETLMRIIDPPVEGYVGGIGRIMHRFKRPEELAKLDELVDEVSRVLDARR
                     DVLAKDPLSVFPPRLMTIIEEEIPEVNFLTHPTPVTVQMEGLRVKLPYDSYAPEIRSL
                     EVAEGVTVGDVAEVKPSRMRNYILIRVKPFSETRILV"
     gene            complement(6788..7372)
                     /locus_tag="PK196_03545"
     CDS             complement(6788..7372)
                     /locus_tag="PK196_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HON40529.1"
                     /translation="MPPLNYFVVESPEEVGRKAYERVAGDVLQDLDLIKVIDRLHIYV
                     DPKIPIFIAAGVLHHMPRTIRVGDFANVNRGDTRIVLDIGDETYLAPLLRVLWEIYGK
                     ENVEQPDRFTVILPAGMAGEEDLEQVVVADPGETLHKDVIYAMQYLAPEGFKVRRQYI
                     RGERFYYVASEDTLPGDVVERMVMPLFERMGVTL"
     gene            complement(7373..8611)
                     /locus_tag="PK196_03550"
     CDS             complement(7373..8611)
                     /locus_tag="PK196_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HON40530.1"
                     /translation="MSRKVRIAQLSCGSEYSGVQNEIYKAAEAVNAEVFFPDIALGDV
                     ERSFDAFGLDVRSADLKLMIARGMALVEGKVDADAVFICTCFRCAEAAIVRNELRRYI
                     HENSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREAQTGITMGIDSGSSTT
                     KAVVMKDNRIMGTGWLPTIEVIKSAEGAVAQALEMSGIAREEIQAIGTTGYGRFLVGR
                     HFGADLIQEELTVNSKGAVYLADHQRGTSTVIDIGGMDNKAISVIDGIPGTFTMGGIC
                     AGASGRFLEMTAKRLGVDITELGPLAMKGLAKKVPMNSYCIVFGTQSLVNALAAGSTR
                     EDVAAAACHSVAEQVFEQQLQEIDIKEPVIMVGGTSLIEGLVHAMGDLLQVKVVVPPH
                     SQYIGAVGSALLASGFVEGE"
     gene            complement(8608..9060)
                     /locus_tag="PK196_03555"
     CDS             complement(8608..9060)
                     /locus_tag="PK196_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HON40531.1"
                     /translation="MAKVFIYPATSLILSDLVARFGHKPLGAALGIRERIQTAGVDSP
                     PLQITPEEPKRGLKYAAVEVPSGVRGRMAIYGPLLDEAGAAIIVNDADLAFGCMGCAR
                     TDELLLFILRQSGIPILELDYPGNEEEGVQFVAAVRKFLDNLPGEVES"
     gene            complement(9066..9527)
                     /locus_tag="PK196_03560"
     CDS             complement(9066..9527)
                     /locus_tag="PK196_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HON40532.1"
                     /translation="MAEYTPDHVGTVTKYVFVESPSMTPGELALRAYEVSEGVLIKET
                     CFGLQVTGEPGAVDRLIEVIRSIDPDHIFIKDRGFPPGDSRRCRANLGGARPGYLGHE
                     REFRLLRYISRGLEELERRGGEEPEPVHPARETRTKLDIRRLQELIESEES"
     gene            complement(9527..11053)
                     /locus_tag="PK196_03565"
     CDS             complement(9527..11053)
                     /locus_tag="PK196_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HON40533.1"
                     /translation="MEIHLDGKRVEVPEGSCLGDLLPGWDWQCSAAIIRPAVEEDVTE
                     SAEIRFVTTAGDLVVEISDPAVAAHILQPEIAERLRLHWQDRYAAAFGPFKSDIHPAR
                     EPGHYERGDVILGCGGYDPFRSYLIFARIRHVADHGAPRGGGVIGRVVTGRGVIDRFG
                     EGDQITGVERVLQRADRSHATITHDAGFLLEDGMQVISYLEVDVQGFSKEEIDTTAAR
                     SVEYFLLSVQDGRFKVDRSSSTYISDTRLVPTAVPTEFPGPRLEGTVTVRTSGKSRGA
                     VYIYTQAVSPSPVHTVVGRVAHGIDLARFAGEGDLLAIRVEPERFDLIGLAVDEAKAV
                     AARRGVTLTPDVAGGDRIVVGQSPETTLEVLAAGEVRVETESPDRVIAVTLYEAAAPR
                     SVKIFREVTGLKHHAIGKMPLIFIFEDVYLFKPKIEKNVGIIPENTPTEEVPAFMLAM
                     TNDSRRGVGMVGVRTVASAEFGPTSEPFTGTNIIGKVLDPEKLAGLREGSTVYVREVK
                     "
     gene            complement(11064..12677)
                     /gene="atwA"
                     /locus_tag="PK196_03570"
     CDS             complement(11064..12677)
                     /gene="atwA"
                     /locus_tag="PK196_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HON40534.1"
                     /translation="MTPLITVKDLCMEFNGTTVLKNINFEVAEGETVGIIGRSGAGKT
                     VLMHLMRGVDQPPTQGVIIYHIAACEGCDYVGTPRERGKPCPACGGELKPADIDFWAE
                     ENAPMKRRIMRRTAIMFQRTFALYGDDRVIENVLRALDDIGYPSEKAISRAADLIDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKNPFILFADEPTGTLDPETATLVHRMLIES
                     AQANNMAMVVTSHFSHVIEEVADRAILLENGEIVEIGDPESVIKRFMENYSDIEQHRA
                     AVVGEKILVARDVVKRYLSIDRGVVRAVNGVSFEVYEKEIFGIIGKSGAGKTTLSRII
                     AGILEPTSGEMNIRIGDEWIDMTKPGIENRGRAKAYIGLLHQEYDLYPHRTVLDNLTD
                     AIGLEFPKELAMRKAVITLGMAGFTPEKSREILDRYPGQLSEGEKHRVALAQVLIREP
                     RLVVLDEPTGTMDPITKLDVKHSILHSREEMDETFIVVSHDIDFVRDICDRVALMRGG
                     KIIQMGPTEEVLAHLTDDERKVMGGAETT"
     gene            complement(12718..13569)
                     /locus_tag="PK196_03575"
     CDS             complement(12718..13569)
                     /locus_tag="PK196_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HON40535.1"
                     /translation="MLVAVVFDSAGTLLRTYRVARDVLRGEMLTGVETTTLTFGAEAR
                     VLVVLHLHSRDVMDAPGDQLLSEFLSERSIGFGVACSRRVIPAEEVAHVLYSDKNARV
                     SDLQACIRKVWSCCKRESIITMNSGVILNMDLPGIEFTVTTGGRPFDGAREAISELHR
                     MGVPVYIASGDRLEKLERMGDHLGIPRERIYGVATPSMKARIVEELKSQYDRVVMVGD
                     AINDLNAFQKADLAVLTEQQSDRKPDILYATVDRVIRNVSEVPGIVAELLSGAPATGK
                     KVQQYKP"
     gene            13625..14554
                     /locus_tag="PK196_03580"
     CDS             13625..14554
                     /locus_tag="PK196_03580"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HON40536.1"
                     /translation="MLLSETAESIKNMEIRGAGRIARAAVEALADYARSLDVSDPAVF
                     RQEMDKAAGILIETRPTAVSLPNAVRSVIRALDEFESVEAAKKAVLSRATEFVQRSEH
                     AVERIAEIGARHISDGDILLTHCNSEAALGCILEAHRQGKEIEVYATEVRPRGQGLIT
                     IRTLNEAGIPTNYIVDSAVRHFINEVDMVFVGADAIAVNGAVVNKIGTAQIALAAHEA
                     RTNFIVAAETYKFAPRTILGELIEIEERDPAEVLPREVAEELPYVRVRNPAFDVTPAD
                     YIDLIVTEQGAIPPELAFTVIRDYLGWTIEELQ"
     assembly_gap    15071..15170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15318..15803
                     /locus_tag="PK196_03585"
     CDS             15318..15803
                     /locus_tag="PK196_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HON40537.1"
                     /translation="MYQTKHDHCVSLPPDTPLAVSISIIHRLHAIYLNRVLKQINLTA
                     GQVPFLFQIAHSPGITQDEIAATAHIDKGTVARAVKKLEDGGFISRTPDALDRRRYHL
                     SLTERGEAAIPLITATDRAWEDLITSGLSGGERSQLYVGIRRLAANSIETIRVCGGER
                     A"
     gene            15800..>17060
                     /locus_tag="PK196_03590"
     CDS             15800..>17060
                     /locus_tag="PK196_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HON40538.1"
                     /translation="MSSHPPAADAHSGVAIMTGDPKRALLKLSGPMIVAMLMMSLYNL
                     ADAIWVAGLGDDALAAVGFITPFFMILIGLGNGLGAGANSAISRCIGARNKKGADNAA
                     MHALILTAALSILLTILLVPFLETLISAIGAGGATGLAVEYGEIVFAATIFLLFNSVA
                     YGLLRAEGDAKRTMYAMTASAILNIVLDPILIYTAGLGIAGAAWATVISIVVVSLIML
                     YWFFVRHDTYLTISWKNFSHDRGVHADILKVGLPASLEMFLIAGVTVIINGILVAVSG
                     NDAVAVYTSGWRIVMIATVPIMAIGTALITVAGAAFGGRDYRNVATAHSYATLIGFGA
                     AIAASALTLVFAPWIAALFSYSEESAHLAPAIVGFLQVMCLFYIFMPPGLMSSSLFQA
                     TGNGVTALVLTLLRELICIGLFAYLFAI"
BASE COUNT         3573 a         5200 c         4693 g         3494 t
ORIGIN      
        1 tgatgtatct atcgcaggaa ggaagggcgg ctccgctttc atccccggcg gcaaggcggg
       61 ggtcttcccg cttcaccccc ttcacccccg caagttaaaa gacagtccgg ttcccggcag
      121 cgaacatagc aattgcgccc tgcctcaggc gcttttagtg acgcaccctg ccctgaactc
      181 ccgctgagtt gcctgaacgg ggcagcctct actggcaata caggtgcaca cgaactgtct
      241 ttccttcgtc ggaccaaacc ccgggacgcc ggagcgtttc cgtgttttcc gtggatctcc
      301 tcactaccat tggcgcggga agaggagccc gatggtgaca cctatccagg aggggtttgc
      361 gggtatgctg ctctcgcctc aggcatcgcc agtcatttat caaggccaaa agaccggtca
      421 atctcagcag ccgcggcctc gaggtcttca agaacacgga gagcgtcttt gcgcaactcc
      481 tgcaccagcg actggagaga gttcccgcgc agatagatcc gcttcctgtg gcggtagacg
      541 acaccggcat cgacaaggtt tcttatgtgg tggttgacac gtgaactgga gatgttcaag
      601 tcacgggata tcctctccac cgatacaccc tctcccgcgc tctggtagga gagaagtgcg
      661 gccaggatcc gcctggcaac ctggtcaacg tctctcccct cacctacacc gaggctgttg
      721 aagagccatt caaggttctc ttccgcccct ctccccctcg gtctctcgat ctgcctgaat
      781 gtaatctgcc ttgccatcgg cgccatcgtt cactcacctg tatggcgccc ctaatgtata
      841 aacatatcca acaatattga aatgatctca ataatttaga taaggtttaa atattcatac
      901 atccgtaaat atttgtaaag ttcctctgaa ggtgataaac atgaagattg tcccaatcgg
      961 agaacgggtt cttatcaagc cgatcaagaa ggaagaagtt acaaagagtg gtatttatat
     1021 ccccgaatca gcacgcgaga ccagaaaaga gggaaccgtt gttgcggcag gcaggcgcga
     1081 tgacggatca gacctcccgc taaaaccagg cgaccacgtt ctctacggtg gctacagctc
     1141 ggacacgttt gagatcgact ctgaaaccta tgttttcgtc ccttttaagg acatcctggc
     1201 aaagatcgag tgaggaggag agggtatggc atcgtcaaag cagttgatgt ttgatgaaga
     1261 cgcacggaag gcgatccttg tcggtgtcaa caaagttgca gacacggtca agatcacgct
     1321 cggcccgaga ggcaggtatg ttgtgatcga caggccaaca aaccccatcg ttacaaacga
     1381 cggtgtaacg attgcaaaag agatctcact gcatgacaag tttgagaaca tcggggcaaa
     1441 acttgtaaaa gaggttgcgc agaacacaca ggacaagacc ggtgatggta ccacaacagc
     1501 aaccctgctc acacaggcca tcctcaccga gggcctgaag aacatatccg caggcgccaa
     1561 ccccaatgat gtcaggcggg gtatagaggc cgcggttgcg gcggcggtgg agtatatccg
     1621 gtcgacaagc gttcctgtca gggagcggaa caggatcctg caggttgcta caatctcggc
     1681 caacaacgac gaggagatcg ggagacttat cgccgatgcg atggataagg tcggctacag
     1741 cggacttatc accgttgagg acgcaaagag ccttgagacc agtctcgacg tggtcagggg
     1801 gatgcagttt gagcagggct acatctcgcc atacatgatc acagacccgg agaagatggt
     1861 ctgcgaatac gaaaacccct acatcctgat caccgaccgg gagatcacct cactcaaaca
     1921 gatgctcccc atcctcgaga tcgccgcaca ggacggcaaa ccgctcctga taatcgccga
     1981 ggatgtcaac ggcgaggcac aggccgcgct catcttaaac atcatacgcg gttcactcaa
     2041 ggcctgcgcg gtcaaggcac ctggattcgg cgatgaacgc aaggaactcc tggaggatat
     2101 cgccgtcctc acgggtgcaa cggttatatc cgaggataag gggatgaaac tggagaacgt
     2161 atccaggcag gtctttggtt cggcccacac cgtcagggtc gacggggaga agaccctgat
     2221 agtcgggggc aagggcgacc gcaaggctct tgaagaccgg atgcgcctga tcgagtctca
     2281 gatcaacatc acagactcag agtggaagaa ggacgaactc aaaaagaggc ttggaaacct
     2341 cggcggcggc gtcgcggtca tcaaggttgg ggcggcaacc gagaccgaac tgaaggagaa
     2401 gaagatgcgg atcgacgacg ccttaaacgc caccaaagca gcggtcgagg agggtgtggt
     2461 tgccggcggc ggcgtcactc tcttccgggc aatcaagaac ctggatgcgc tgcagttcga
     2521 tgacgaccga aagatcggcg tctccatagt tcgccgtgcg ctggaggagc ccctccgcca
     2581 gatcgcagag aacgccggtg tcgagggcgc tgaggttgtc gccaccgtga agaggagtga
     2641 cgatcccgct ttcggctaca acgccaagac cgagacctac gaaaacctga tggagaacgg
     2701 tgtcatcgat cccgcgaagg tggtgcgact cgggctccag aacgccgcct ccattgcagg
     2761 gctgatcctc tcaacggagg ttgctatcgc cgagttcgat gatgagaaag atacaaagac
     2821 agcgacaatc atcatctgag gcaaattgcc tcttttcctc ccacggttca tgaaaagtga
     2881 ggggattcgg gtggttctgg accgcccctt caaccccctg ctacaggaag cctggcagcc
     2941 tgccaggcgc tgatcccgcc ctctatctcg tagacctcgc gaaacccggc ctcattcatc
     3001 aacttgtgaa caccagcgct cctcgtcccc ctccggcagt atatcacgta ggttccgtcc
     3061 gggttgaggc ttttgaggtg ttcgaggatg gtcgccgcgt cgatgttgat cgcgcccggg
     3121 atgtgccccc cggcaaactc gtccgccgtc ctgacatcga tgatgacaaa acccgggtcg
     3181 ttctccatat cttcgatcat tatggcggcc tcggcaggat ggatcacctt agaacccggt
     3241 ttttccctgt ccggatcact tccgagacac gcacaggcga gggtaatgcc gagaacggca
     3301 aggagcagcg gcggaatacg aaaggtatcg accatagttc catatcaccg gcagcaacga
     3361 taaagaggtt tcggtcatcc gccatcctca cccacgggct tccccccccc cgctgccagg
     3421 tctggtcgtt tcaggccgaa ttatagattc aaacggtgtg ccatcgatgg ccatcagcag
     3481 acggaacgcc ccggcggcat ccagggccac gagcggaaga tgctgatgcc acgctcagca
     3541 acgtctcaga taactgcact tccctcttca aacccccgga gccacagttc cgcacaggct
     3601 atccgccaga actcggtgga gtaggcactc ttccccgcaa gaaatttccg atagtttcgg
     3661 agcacgcgct ctgcatccca gtagggcctg ccagaaaact ccggcgagga gaagagttca
     3721 aggacgcggg gtgcaagctc ccccctcatc cagacctcct cgggggtgac aaaacccatc
     3781 ttgtcgctcc tgcagcggac ggggtcaggg accagccccc tgatcgcccg ccgcaggacg
     3841 tactttgtga caccaccacg tatcttctgg tcgagcggga gcgacgcaag gtactccaca
     3901 acccggtaat ccagaaaagg aacccgggcc tcgatcgaga aggccatcga gttccggtcc
     3961 tcccagtgga gcagtagtgg gaggttggag accgtgatct cccgtttcag cacctcatca
     4021 agcgttcctg cgtagcggag aacctccggc ggagacccct ggaggagatc acgccgctgc
     4081 gaccggacaa caacctggcg gagcgcccac cagaagaacg atcggtgcaa cctcaagatc
     4141 ccgataacct ccctgagggc tgccaggatc tcgccgtgcc caagaagggt gcggatatag
     4201 ggcaccaggt aagcaatgta gcccccaagt tgctcatcgg cgccctgtcc gtcgagaacc
     4261 accttcacct cctcccgtgc aagccgcatc acgcagtact gggcgtagat cgagagtgac
     4321 gcaaacggct cgtcctgcat gtagagcagc cgctcgatat cctcccagag accgtcagtg
     4381 ccgggggtcg tccggctgtt ctcgaccccg gtcgccgcaa ccaccgtatc aatgtagcgg
     4441 ctctcatcaa acctcccgtc ctcgaaacag accgagaacg tcttctgccg gtcgccgacg
     4501 ctctcagggc tctctgaacg cagcaaatgg ttgatcagga cggttacagt tgatgagtcg
     4561 atcccgccgg agaggcacgt cccgacgggg acgtcgctcc tgagcctgag acgaacagca
     4621 tccgtcagga ggttcaggag gtctcgtgct gcagcctcat cgtcgacccc ggccccacgc
     4681 gggttagggt tcatctcgaa atcccagtag cgctccggct tcccaacacc cttttcggtg
     4741 accaagaggg tgtgggctgg cggaagctgg aagataccct catacatcgt cgctggagta
     4801 tggtccgcga caccccacgc cagaaacgcc aggagcatca ggtcgttcgg tcgtttcccg
     4861 gcatcagggt gtgcccggag tgccttgatc tccgatgcaa atagaaacga acccccgacc
     4921 accgtgtagt agaatggctt gacacccagg cggtcgcggg cgcagaagag ttcacggcgg
     4981 cgcccgtccc agatggcaaa agcccacatc ccgttgaacc gctgcaggca gcccctcccc
     5041 cactcctcgt aggcatggag gatcacctcc gtatcggtcg cggagaggaa agagtgtccg
     5101 gcagccagga gttcgtcccg gagctcgcgg tagttgtaga tctcaccgtt gaagacgatc
     5161 catatcgacc cgtcctcgtt tgccatcggc tgccgtccct catcagaaag gtcgattatg
     5221 gccagacgcc ggtgggcgag gccaaccggg ccggagcata agcttccctc cccatctggc
     5281 cccctgtggg caagcttctc tgccatcgca gcggtgaggg atggatcagc ctctccccca
     5341 ttcaggacaa actgcccggc aataccacac atgctcagga catctggttg tcggtcattt
     5401 atccgaacag gtcttattca accctactcc gttgagatcg catgtggggc aggatgtcga
     5461 cgacacgtcc accacacggt atgcatccgc aagaaccgcg gagtttgcga gcatccccgc
     5521 gatcaggatc acctcaagga tctcggcccg ggtcgcgccc tttgccatcg ccgtctgcat
     5581 gtggtactca acacagtggc tgcacttgag cgcagcccca accgcaaggc tgatcagttc
     5641 gatcaccttc ggctcgagct ggctggtctc agagaccgcc cccttgtaaa ggaggtgtga
     5701 gatgaggatc tcgggtcgtt ccgccatccg ctcaaatatc aggggaaccc tcccgtactc
     5761 acgctcgatc tcctggagga gctcctccgc gatggcatct ggcccgcgtt ccataatcct
     5821 cgtgagtatc ttctcaaagt ccatcgcaac tacaccaata tccgggtttc cgagaaaggt
     5881 ttcaccctga tcaatatgta gttgcgcatc cgtgacggct taacctcagc gacatccccg
     5941 acagtgacac cctcagcgac ctcaagcgac ctgatctccg gagcgtagga gtcgtaagga
     6001 agtttcaccc tgagcccctc catctggacg gtgaccgggg tcgggtgcgt caggaagttg
     6061 acctcaggga tctcctcctc gatgatcgtc atcaaccgcg gcggaaaaac ggataggggg
     6121 tcctttgcaa ggacgtcccg gcgtgcatcc aggacacggg aaacctcgtc caccagctca
     6181 tcaagtttag cgagttcctc agggcgcttg aacctgtgca tgatcctgcc gatgccccca
     6241 acataccctt cgaccggcgg gtcgatgatc cgcatcaatg tctcgcggtc gggagcaccg
     6301 gtcaccacga ttggaacgtg gatcccacgg cggagcaccg ggaacttatg ctgtatacac
     6361 tcctcaaagt tgccgagcat gtatacagcg aggtcgtgct cgttgatgac gtcccgttcc
     6421 tcgtcattga ggtttgcaat ccgcttcccg aaccccctgg caagcccgac catattggtc
     6481 tttgcccccg aacgcctcag gtactcggcg atatcgcagg aggtgtgggg gaggtgatgg
     6541 atctcaaggc tcgggctgac aacagcgatc tctgtcccga caaggggcgc ctcggtcacc
     6601 tgcccggcaa gcggtttggc aaccgcgcgt atcaggttga tatcctcgcg gggcacgaac
     6661 gactggagca cgacgtcctg cgccataacg tgtttctgca caacatagcc gccaaggtca
     6721 tcgatcaggt ccatgatctc gtcgtgccgg tagacgccgc ccttgtatgt caccggaacg
     6781 agcgttgtca cagcgtcacc cccatcctct caaagagcgg catgaccatt ctctccacaa
     6841 cgtcaccagg cagggtatcc tcgcttgcca cgtagtaaaa cctttcaccc cggatatact
     6901 ggcgacggac cttgaacccc tcgggagcca ggtactgcat cgcgtatatg acatccttat
     6961 gcagcgtctc gcccggatcg gcaaccacca cctgctcgag atcctcctcc cctgccatac
     7021 ctgccggcag aattacggta aaccggtcag gctgctcgac gttctccttg ccataaatct
     7081 cccagaggac cctgagcagc ggcgcaagat aggtttcatc accgatatcc aggacgatcc
     7141 ttgtatcacc gcggttgacg tttgcaaagt caccgacccg gattgtgcgc ggcatgtggt
     7201 gcaggacacc ggcggcgatg aagataggga tcttcgggtc aacgtagata tgcagacggt
     7261 cgatcacctt gatgagatcg aggtcctgga gaacgtcgcc tgcaacacgc tcataggcct
     7321 tcctcccgac ctcctccggc gactcaacaa cgaagtagtt taacggaggc atctactccc
     7381 cttcaacgaa ccctgaagca agcagggcag acccaaccgc accgatatac tgcgagtgcg
     7441 gcggaacaac gacctttacc tgcaggagat cgcccatcgc gtgaaccagg ccctcgatca
     7501 gggatgtgcc gccgaccatg atcaccggtt ccttgatatc gatctcctgc aactgctgct
     7561 cgaatacctg ttccgccacg ctgtgacagg ccgcggccgc aacgtcctcc cgcgtgctac
     7621 ctgcggcgag cgcgttcaca aggctctgcg tcccgaatac gatacagtaa ctgttcatcg
     7681 ggaccttctt cgccagccct ttcatggcca ggggaccgag ctcggtgata tcaacaccaa
     7741 gacgttttgc ggtcatctcc aggaaccggc cgcttgcccc ggcgcagatc ccacccattg
     7801 taaacgtccc cgggatacca tcgatcacgc tgatcgcctt gttatccatc ccgccgatat
     7861 ctatgacggt tgaagtacct cgctggtggt cggcgaggta gacggcaccc ttcgagttca
     7921 ctgtcagttc ctcttgaatc aggtcggccc caaagtgcct gccgaccagg aaccgaccgt
     7981 aaccagtcgt cccgatcgcc tggatctcct cgcgggcaat accggacatc tcaagcgcct
     8041 gggcaacagc accttcggcg ctcttgatca cctcgatcgt tggaagccag ccggttccca
     8101 tgatcctgtt gtccttcatc acgaccgcct tcgtggtgct cgaaccagag tcgatcccca
     8161 tcgtgatccc ggtctgcgcc tcgcgggcga gaagcgccct ccggcgggct atagtggtca
     8221 gcgcctccat ccgggtgagg agggttcccg ctgtcgtccg ctcggtgaag gagtaactca
     8281 ccaccggcag ccttgaattt tcgtggatgt agcgccggag ttcgttccgg acgatcgccg
     8341 cctcggcgca ccggaaacag gtgcagatga aaaccgcgtc ggcatccacc ttcccctcca
     8401 ccagcgccat gccacgggca atcatgagtt ttaaatcggc gctccgaaca tccaggccga
     8461 acgcatcgaa cgatcgctca acgtcaccga gagctatgtc ggggaagaag acctctgcat
     8521 tcaccgcttc agcggccttg tagatctcgt tctggacacc actgtactca gatccgcagg
     8581 agagctgcgc gatccgcacc ttccggctca agattcaacc tcccctggaa gattgtcaag
     8641 gaacttcctg acagccgcta caaactgcac accctcttcc tcgttcccgg gataatccaa
     8701 ctccagtatc gggataccgc tctggcgcag gataaagagg agcaactcat ctgttctggc
     8761 gcatcccatg cacccgaacg cgagatcggc atcgttgacg atgattgcgg ccccggcctc
     8821 atcgaggagc gggccgtaga ttgccatcct gcccctgaca cctgacggca cctcgactgc
     8881 cgcgtatttc aggccgcgct ttggttcttc gggcgtgatc tgcaggggcg gggagtcgac
     8941 cccggcggtc tgtatccgct cccggatgcc gagggctgca ccgagcggtt tgtggccgaa
     9001 cctggccacc aggtcggaca ggatgaggct cgttgcgggg tagataaaca cctttgccat
     9061 tgaattcagg actcctctga ctctatcagt tcttgtaatc gccttatatc gagttttgtt
     9121 ctcgtctcac gggcaggatg caccggttcg ggctcctcac ccccccgtcg ttcgagttcc
     9181 tcaagtccgc ggctgatgta gcgcaggagt cgaaactccc gctcatgccc gagatagccc
     9241 ggtctcgccc cgcccaggtt cgccctgcag cgtctcgaat cacccggagg gaaacccctg
     9301 tctttgatga agatgtggtc tggatcgatg ctgcggatca cctcaatgag tctatcgacg
     9361 gcacccggtt caccggtcac ctgcagccca aagcaggtct ctttgatcag gacaccttcg
     9421 gaaacctcat aagcccgaag cgcgagttca ccgggcgtca tgctgggtga ttcgacaaaa
     9481 acatattttg taaccgtacc tacgtggtcc ggggtgtatt cagccatcat ttaacctccc
     9541 ttacatacac cgtcgagcct tcacgcagcc ccgccagctt ctcgggatcg agcactttgc
     9601 cgattatgtt cgttcccgtg aacggctcag aggtcggacc aaactcggcg ctggctacgg
     9661 tccggacacc gaccatgccc acccctctgc gagagtcgtt cgtcatcgcg agcatgaacg
     9721 ccggcacctc ctctgtgggt gtgttctcag gtatgatccc gacattcttc tcgatcttcg
     9781 gtttgaagag gtagacatcc tcaaagatga agataagcgg catctttcct atggcatggt
     9841 gtttgagccc ggtcacctca cggaatatct tgaccgacct tggtgcggcc gcctcataga
     9901 gcgtaacggc gatgacacga tccggtgatt cggtctcgac cctgacctca ccggcggcca
     9961 gcacctcaag tgttgtctcc ggactctggc cgacaacaat gcgatcacca ccggctacat
    10021 cgggggtgag ggttacacca cgtctggcgg cgaccgcttt tgcctcatcc accgcaagac
    10081 cgatgagatc aaaccgttcc ggctccaccc ggattgccag gaggtcgccc tccccggcaa
    10141 accgggcgag gtctataccg tgggcgaccc ggcctacaac ggtatgtacc gggcttggtg
    10201 atacagcctg cgtatagatg tagaccgctc ccctcgattt tcccgacgtc ctgaccgtga
    10261 ccgtgccctc gagccttgga cccgggaact ccgtcggtac ggctgtcggg acgaggcgtg
    10321 tatcggagat ataggtgctg ctcgaacgat ccaccttgaa tctgccatcc tgcactgaga
    10381 gaaggaagta ctcaacgctt ctcgcggccg tggtatcgat ctcttctttg ctgaagccct
    10441 gcacatcaac ctccagatag gagatgacct gcatcccatc ctcaagcaga aaccctgcat
    10501 cgtgggtgat ggtggcgtgg gagcggtcgg cacgctggag cacacgttcg acccccgtta
    10561 tctgatcacc ctccccgaac cgatcgatca cgccccggcc ggtgacgacc cggccgatga
    10621 caccgccccc tctcggggca ccgtggtcgg caacatgcct gatgcgcgcg aagatcaggt
    10681 atgacctgaa gggatcgtag ccgccgcatc ccagtatcac gtctccgcgc tcgtaatgtc
    10741 ctggttctcg tgccgggtgg atatctgact tgaacggccc gaatgcagcg gcgtagcgat
    10801 cctgccagtg caggcggagc ctttcggcga tctccggttg aagtatatga gcagccaccg
    10861 cgggatccga gatctcgact accagatccc ctgccgtggt gacgaacctg atctccgctg
    10921 actcggtcac gtcctcctca accgccgggc gtatgatggc ggcgctgcac tgccagtccc
    10981 accccgggag caggtcgcca agacacgaac cctccggaac ctcaacacgt ttgccgtcca
    11041 ggtggatctc catcacgcgc acctcaggtg gtttcggccc cacccatcac ctttcgctca
    11101 tcatcggtta ggtgagcgag cacctcttcg gtcggaccca tctggatgat cttgcctccc
    11161 cgcatgagag ccacgcgatc acagatatct cgcacaaaat caatgtcgtg tgagaccacg
    11221 ataaacgtct catccatctc ttcgcgggag tggaggattg aatgctttac atcaagcttc
    11281 gtgatagggt ccatcgtacc cgtcggttca tcaaggacga cgagacgggg ttcgcggatg
    11341 agcacctgag cgagagccac ccgatgtttc tccccttcag agagctgacc cggataacgg
    11401 tccaggatct ctctgctctt ttcgggtgta aacccggcca tcccgagcgt gatgaccgcc
    11461 tttcgcattg ccagttcttt tggaaattca agaccgatcg catcggtaag gttgtccagc
    11521 acggtcctgt ggggatagag gtcatactcc tggtggagga gcccgatata ggccttcgcc
    11581 cggccacggt tctcaatacc gggttttgtc atatcgatcc attcgtcccc gatccggatg
    11641 ttcatctcgc cgcttgtggg ttcaaggatc ccggctatga tccgggagag ggtcgtcttg
    11701 cccgcaccgc tcttgccgat tatcccgaag atctccttct cgtagacctc aaaggagacg
    11761 ccattgaccg ccctgaccac accacggtcg atcgagagat aacgcttgac gacgtcgcgg
    11821 gcaaccagga tcttctcacc gaccactgca gccctatgtt gctcgatgtc gctgtagttc
    11881 tccatgaacc tcttgatcac gctctcggga tctccgatct ccacgatctc cccgttttca
    11941 agcaggatcg ctctatctgc gacctcctca atgacgtgtg agaagtgcga cgtaaccacc
    12001 atggccatat tgttcgcctg cgcgctctcg ataagcatcc gatggacaag tgtagcggtc
    12061 tccgggtcga gcgtcccggt aggctcgtct gcaaaaagga taaacgggtt tttggcaagt
    12121 tgccgggcga gcaccacccg ctgtttctcg ccgcccgaga ggtcacgggc aatgtgcatc
    12181 atacgatggg agagtctgac ctggtcgatg aggtcagccg cccggctgat ggccttctcg
    12241 ctcgggtaac cgatatcatc gagcgcccgc aggacgttct cgatcacacg atcgtcgcca
    12301 tagagcgcga acgtccgctg gaacatgatg gcagtccttc gcatgatccg acgtttcatc
    12361 ggtgcgttct cttcagccca gaaatcgata tccgccggtt taagttcgcc accacaggcg
    12421 ggacacggtt ttccccgctc acgcggtgta ccgacgtaat cacacccctc acaggctgcg
    12481 atgtggtata tgattacacc ttgcgtcggg ggctggtcaa caccccgcat caggtgcata
    12541 agaactgtct tgccggcacc gctccttcct attatgccga cagtctctcc ctccgctacc
    12601 tcgaaattaa tgtttttgag tacggtagtt ccgttgaact ccatgcagag gtctttcacc
    12661 gtaataagtg gggtcatatt gaggtcctcg ctaaactgat ctttctgtgg tatcatatta
    12721 gggtttatat tgttgcactt tcttcccggt tgccggggcg ccggagagta gttctgccac
    12781 gatgccaggc acctcgctga cgttgcggat cacccggtca acagtcgcat aaagaatatc
    12841 cggtttccgg tcggactgct gctctgtgag caccgcaagg tctgctttct ggaaggcgtt
    12901 aaggtcgttt atggcatccc ccaccataac aaccctgtca tattgagatt ttagttcttc
    12961 cacgatcctt gccttcatcg agggggttgc aacaccgtag atccgttccc tggggatgcc
    13021 aagatggtcc cccatccgct cgagtttctc aagccggtcg cccgatgcga tgtatactgg
    13081 aacgcccatg cggtgcagtt cgctgattgc ctctctcgca ccatcaaaag gtcttccgcc
    13141 ggttgtcacc gtgaactcta tgccggggag gtccatgtta aggatcacgc cgctgttcat
    13201 ggtaatgatg gactcccgct tgcagcacga ccagaccttg cggatgcacg cctgcaggtc
    13261 gctcacgcga gcgtttttgt cgctgtagag gacatgggcg acctcttcgg cggggatgac
    13321 cctcctggag caggctacac cgaacccgat cgaacgctcg gagaggaact cagacaggag
    13381 ttgatctcct ggcgcgtcca tgacatcacg cgaatggaga tggagaacga cgaggacccg
    13441 tgcctcggca ccgaaagtga gtgtcgtggt ctcgaccccg gtcagcatct cgccgcggag
    13501 gacatcgcgc gctacgcggt atgtccgcag gagtgtgccc gcactatcaa aaacaacggc
    13561 taccaacatc tgctctcacg tcaataaatc tatgtggtct gaggtcatct cttgagtgag
    13621 aaccatgcta ctgagtgaga cggcagaaag cataaagaat atggagatcc ggggcgccgg
    13681 caggatcgca cgggcggcgg tggaagcact ggctgattac gcgagaagtc tcgatgtatc
    13741 cgacccggcg gtattcaggc aggagatgga taaagcggcc gggatcctta tcgagacgag
    13801 accaaccgcc gtctcgctcc caaacgctgt aaggagcgtt ataagagcgc tggatgagtt
    13861 tgagtcggtg gaggccgcaa agaaggctgt cctttcacgg gcgacggagt tcgttcagcg
    13921 ctccgagcat gcggttgagc ggatagcaga gatcggggca cgccatatct ctgacggtga
    13981 tatcctcctt acccactgca actccgaggc ggcgctcggg tgcatactgg aggcacatcg
    14041 tcagggtaag gagatagagg tctacgcgac ggaggttcga ccgcggggtc agggtctcat
    14101 cacgataagg actctgaacg aggccggaat ccctacaaac tacattgtcg actcggcggt
    14161 ccgccacttc atcaacgagg ttgacatggt ctttgtcggt gccgacgcga tcgcggtgaa
    14221 cggggcggtg gtaaacaaga tcgggaccgc ccagatcgcg cttgcggcac acgaggcaag
    14281 gacaaatttc atcgttgccg ccgagaccta caagttcgcg ccgaggacga tcctggggga
    14341 gttgatcgag atcgaggagc gtgaccctgc cgaggtgctg ccgcgagagg ttgcagagga
    14401 actcccctat gtcagggtga ggaaccctgc tttcgatgtg actcctgctg actacatcga
    14461 cctgatagtc acagagcagg gggcgatccc gccggaactt gcgttcacgg tgatccggga
    14521 ttacctggga tggacgatcg aggagttgca gtaggtccgt cggggggcat acaggacgct
    14581 caagggacag gggttttgct ccatcccgga aatatacagg taataacctt tttttcaccg
    14641 ttaaagcctt tcggcttttg tgggtgttgt atggtgccct ggtgcggtga aaaggcaact
    14701 cacaacaccc tggctgatgg cgtgagtggg agtttcgtgg agcgttactc actcttatcc
    14761 gtgcccgggt ctccctccga ctaaatggag agatgagacg acgaaccagg cacaacaaaa
    14821 gtgcaccggg ttgccataac ccttctgaac tgtaacggca caggggtccc cctcagggga
    14881 ggggttcatg tgagtgtgtc tggaatagat aacccgccta atccaacact acgcccctca
    14941 ccctggagga gatactgtcc atcggacggt caaccctccc gcacgtgggc ggggcttatc
    15001 ggagttcacc tgattggatg ccttcatacg gatttacctg acggcggcgc gtttcaccca
    15061 cctgcttaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggatgccttc
    15181 atacggattt acctgacggc ggcgcgtttc acccacctgc ttagaagacc ccggcgcctg
    15241 ccatggtggg taagaccagt tgcatatgca acagatttat ctccagggaa attcacctct
    15301 aatgacccag gatcatcatg taccagacca aacacgacca ctgcgtgtct ttgccaccgg
    15361 atacgcctct tgctgtatcc atctcgatca tccaccggtt gcatgctatc taccttaaca
    15421 gggtgttgaa acagatcaat ctcaccgccg gtcaggtccc ctttctcttc cagatagcgc
    15481 actctcctgg catcacccag gatgagatcg ctgcaacggc ccatatcgac aaggggaccg
    15541 tcgcccgcgc cgtaaaaaag ctcgaagatg ggggttttat ctctcgcacc cccgacgccc
    15601 tggaccgccg caggtaccac ctcagcctta cagagagggg tgaagcggcc atacccctca
    15661 tcaccgcgac cgatcgggcg tgggaggacc tgatcacatc gggactctcg ggaggggagc
    15721 gtagccaact ctacgtgggc atacgcaggc tcgccgcaaa cagcatcgaa acgatcaggg
    15781 tatgcggggg ggagagggcg tgagttcgca tcctccggcc gcggatgcac actccggggt
    15841 tgccatcatg accggcgacc cgaagagggc gctcctcaaa ctctcggggc cgatgatcgt
    15901 tgcgatgctc atgatgtcgc tctacaacct ggccgacgcc atctgggtgg cgggcctcgg
    15961 cgacgacgcc cttgccgctg tcggattcat cacgccgttc ttcatgattc tcatcggtct
    16021 cggcaacggt cttggggccg gggccaactc tgcgatttcc cggtgtatcg gagccagaaa
    16081 caaaaaagga gcagacaatg ccgcgatgca cgcacttatc cttaccgccg ctctctccat
    16141 cctcctgacc atcctcctgg ttccatttct tgagaccctg atctctgcca tcggtgcggg
    16201 tggagccacc ggtcttgcgg tcgagtacgg tgagatcgtc tttgcagcca caatcttcct
    16261 gctcttcaac agcgtcgcat acggcctcct ccgggccgag ggggatgcga agcgaacgat
    16321 gtatgccatg accgcctctg cgatcctcaa catagtcctc gacccgatcc tgatctacac
    16381 cgcaggtctc gggatcgccg gtgcagcgtg ggcgacggtg atctcgatcg ttgtggtcag
    16441 cctaatcatg ctctactggt tttttgtccg ccacgacacc tacctcacca tatcgtggaa
    16501 gaacttctcc cacgaccggg gtgtgcatgc tgacatcctc aaggtcgggc tgccggcaag
    16561 ccttgagatg ttcctgatag caggtgtcac cgttatcatc aacggcatcc tggttgctgt
    16621 ctccgggaat gacgcggtcg cagtctacac ctccggatgg cggatcgtga tgatcgcaac
    16681 cgtccctata atggctatcg gtaccgccct catcaccgtg gcaggggccg cattcggagg
    16741 cagggactac agaaacgtag cgaccgccca cagttatgcc accctgatcg ggttcggcgc
    16801 cgcaatcgct gccagtgcac tgacactggt ctttgctccc tggatcgcgg cactcttctc
    16861 atactctgag gagagcgcgc accttgcccc ggcgatcgtg ggtttcctcc aggtgatgtg
    16921 cctcttctac atcttcatgc cgccggggct catgtcgagc tcgctctttc aggccacggg
    16981 aaacggcgtc accgcgcttg tgctgacgct actcagagaa ctcatctgca tagggctttt
    17041 cgcctacctc tttgccatcc
//
LOCUS       DAOSZX010000025        16644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2881_length_16644_cov_20.098499, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000025 DAOSZX010000000
VERSION     DAOSZX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16644
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2881_length_16644_cov_20.098499"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK196_03595"
     CDS             <1..289
                     /locus_tag="PK196_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HON40539.1"
                     /translation="KSFIAVIAARFLLDGLDNRDLQIGEKSFHIRGYSADSLDECIIA
                     AVQADREGALGIVCAPILAPIVEKIVDCPVGIIKPHQQTLIEAIETIARRI"
     gene            complement(843..1172)
                     /locus_tag="PK196_03600"
     CDS             complement(843..1172)
                     /locus_tag="PK196_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HON40540.1"
                     /translation="MQNGVWITLFFAGILEAGWAIGLKYTEGFTRVWPSAATLVLMGG
                     SIYLLSRSLTGLPLGTAYAVWTGIGAVGTVIAGLVLFDESRSALRLLCILLIVAGIVG
                     LKLCSDA"
     gene            complement(1322..1474)
                     /locus_tag="PK196_03605"
     CDS             complement(1322..1474)
                     /locus_tag="PK196_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40541.1"
                     /translation="MTVEVYEEHRQNAPVEGRRAIGHLERQPHPGVTVIPRYRAGEQG
                     GSLRSR"
     gene            complement(1606..2058)
                     /locus_tag="PK196_03610"
     CDS             complement(1606..2058)
                     /locus_tag="PK196_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HON40542.1"
                     /translation="MIADKARFRAARKLERAAGFRLPDHVFSGGFLEAVGRGVNFENL
                     DRRMQENLLAIFRDFMDCKCKNAPYCGCPERKFTLTIIELREMGLDHRQISAHLLEEY
                     GIDLYPADILSFLEDSVHVLEAIRDVAELQDREKVAESAIEHIKKIEQ"
     gene            complement(2055..2693)
                     /locus_tag="PK196_03615"
     CDS             complement(2055..2693)
                     /locus_tag="PK196_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HON40543.1"
                     /translation="MAKKSAKKTAAKEAEPMKLFYIFYNKERWDNWIRRLEDASFEPA
                     EGEEVSEGEQILYGFAEDLTLSVLKIIRLYQNGRLTKEEAIEKLNDVEVLVMTGLPEG
                     RLEDLVGSLQLSLIVLFTACRKYLDGGFDTDIKTLVKKGKAIGDTNLDEALEIAANIG
                     AAVIDGATCCAKYIKDDAESLSLFDDWLIEIETMSNAMKSLAKFDEEPGEAA"
     assembly_gap    2760..2859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2860..4097
                     /gene="thiI"
                     /locus_tag="PK196_03620"
     CDS             <2860..4097
                     /gene="thiI"
                     /locus_tag="PK196_03620"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HON40544.1"
                     /translation="NQAHDRVMIGGEPVEAVMVRYGEVFLKSERVKRRFVSAMAENIG
                     LALEAEGLAHRIETHRGRILIYGDDPARIAETAARVFGVVSASVCTVTTADIEGITTA
                     ALKRAERRLRPGMSFAVRARRSGVPGFSSQELAAAVGSAILERFDGVRVDLTHPDYEV
                     SVEAREFGGLVYDEKIPGPGGLPYGTQGVVITLLSAGIDSPVASWLMMRRGCRMLHIN
                     MHGGRFGGADVERKVLQNHARLSSWVPGRALDLLVVDMEPFFEVITALKEPRYRCVLC
                     KRFMLRVAGILAEERGAHAIVTGDNLGQVASQTLANMAVISAATTLPVLRPLIGFDKD
                     EVVGRARSIGTFEANPGEIGCAAAPRYPSTAAPAETIARIEEEIDAVGLAERAAASVR
                     VIRAKNGAVEEAASTSDDT"
     gene            complement(4083..5261)
                     /gene="pscS"
                     /locus_tag="PK196_03625"
     CDS             complement(4083..5261)
                     /gene="pscS"
                     /locus_tag="PK196_03625"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HON40545.1"
                     /translation="MRCASDIEARDVEEIYINIDPIQAGGRLTADAMKAVIAYADGYS
                     VCDNCRSPFRLDYIQKPPIAQFHADLARWLNMDAVRVVPGARRGFQAVASSLVERGDP
                     VILASLAHYTEFLAIEEAGGIPREIPKDDSNRITADAAAERIEAVSREFSRTPPLLFI
                     DHVDYQYGNVHDVKGITKAAHQYDIPVLVNGAYTVGIMPVDGKALGADFLVGSGHKSM
                     AAPAPSGVLAAAEEYAGKVFRTTEARGDLTGRTFGIKEVEMMGCTLMGVTVVGMMASF
                     PHVQERVNHWETEVAHSQAVVDSLLSIEGTKVLSEYPRRHTLTRIDTRESFDRVAQQH
                     KKKGYFLSNDLKKRGVIGVIPGSTRVWKFNTFGLTEKQIRHLSEAFIEIARENGLSII
                     "
     gene            complement(5258..5440)
                     /locus_tag="PK196_03630"
     CDS             complement(5258..5440)
                     /locus_tag="PK196_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40546.1"
                     /translation="MSKRAVDAVFQALFLLTDIRFLLRETAPDHELDEGQRERAATTL
                     EKVRRQVAILEEELVG"
     gene            5560..6720
                     /locus_tag="PK196_03635"
     CDS             5560..6720
                     /locus_tag="PK196_03635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HON40547.1"
                     /translation="MKIVHLADTHLGLSAFSRIDPETGMNLREQLIYNNFLAAIDRII
                     AIRPDALVHAGDLFHQVKPKTRAYTTALDALARLHEAGIPMIVVAGNHSMARTRYTAS
                     PFEVLERGGYRAADLYAAHNNRYRRVELGDTVFHLIPNMIEPAGYRRAFEEIEFSGGT
                     NVLVTHGLAAILSDRRLHTVAEHELDATILSDRFDYIALGHYHGQVQVAENAWYSGSL
                     EYCNYGEIDDRKGGLLVNLAAGDIEHIDLPQTPMLNLGRINSDGLTAGEVVASILEAA
                     EARWEEIEGAICQITLDGIRRETLRALDQKALQDLRNRTLDLRLQALTVDDPYHVFHQ
                     DSLAGLDYVTEFEKFVEREHLAPADAEFVKKTGMDVLRSVIARHREGESAAE"
     gene            6707..9880
                     /locus_tag="PK196_03640"
     CDS             6707..9880
                     /locus_tag="PK196_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HON40548.1"
                     /translation="MLLNRLWMKNFKRFRDQEIIFQDGITGIIGNNGAGKSSIVSGIL
                     FALYGLQGTGLDGDYIVSSFAGPQDVCEVRLDFSLSGNDYTVLRRFKRRPSSTLHEAN
                     LYMNQKLLANSVQKVASEIQRIVGMGAGDFRNTIYAGQKELLALLESRAGSRKDWFMQ
                     VLGIDYLKKDSMECLKELIDAREGTCRELSGRLQELDPDGIRGHLLELRTAVAGAEEE
                     AANARTAETGAREELESARRDYERLLDLRERCRELEREEERLTADIERLRAECRDMET
                     EIAARQRLQADLDELQPIVERYEPLKAEVAALAEEKAHAERLNLEAASLEEQIRDHAE
                     RISRLETDLAALAEDERTVAALEEDVRSARALRERIAAMKALQPDYDALREEIARLDE
                     RFAHLEKRVLENRADIQELEEKERDLRALEEETADYESLLARLEVYQEAQEHARQVER
                     SLHEAEAASAAARRIESEISDLSRQIAELEGVEERIEAAERRKDDLTSRISAFAERRE
                     AIRREIQKVAGHREEILAAGPEGTCPMCRQSLGDHYQDLLADLASTAGSLDRALADLD
                     EEYALAVAGQKRLAAELKSLYAQRAALQRLKEQHALAASRYEQSVGLMRRWQAEADEH
                     RAEIRALGLEGYDPAAHESLRERIRVLAGKRAKADTLRGECSRLPVLLEERERLIADA
                     ADHLARRDEAETRLYRLGFDPEALKRLEAEAGALEPVSRAYTEAQTRLARRPALKEDL
                     NAITARLDGLRERLRGVRGELAGLSFDPERFERLSQDYSRAESAHRRALELRVRLEEV
                     PRLAESLGARRDLLAEREAELLRVRAAINELGFDGSMVKAAEERVAGSEQRLAAARER
                     MYAAALRIRSLETEIEHETARLSRAEELVRQRDALTEEIERLKLTRSLTRDYIDYLLQ
                     VVRGRIEEEAGRVLAEITDGRYNTVMLDDDFSVLVHDMGEDYPADRFSGGEQDDIAIA
                     LRVALSRFIAEVNQVHDSTFLIFDEIFGSQDEERRANLLRALRTQEAYFPQIILISHI
                     SEVQDEFSTALLVEMGPDQISQVRVVD"
     gene            9880..10917
                     /locus_tag="PK196_03645"
     CDS             9880..10917
                     /locus_tag="PK196_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HON40549.1"
                     /translation="MDAETAYRQAIRRVAARIRECAPADLSTRFAAAGGFDAASYTHF
                     EPGFEGAVCAVDGSNAIILDAGSFAIAAIRASVSAFARGRRLYRRTTPLHVITVNPGA
                     GNEDFDEIFYDCFRRRPGARLDHDDPARNAAVIRDTLELWSAMEMAAELDTGDLLVLD
                     GTLQVRHASHAEVIEALLNLCNLRGVLIAAVTKRTSLTWGGGHPIVPAAEGLARGVGV
                     AAPWYLSVSGASGLIDRQEARAWKQRGEQYVARLHPRAARAFKVEIPGHYTPDMVDAV
                     FSGLAAYSDDGRVTGYPYPLLDAHLTTRITQDAVEQIRQDIIQGLDRLGMSLADYIGI
                     FGDYHDEFDRY"
     gene            10895..12493
                     /locus_tag="PK196_03650"
     CDS             10895..12493
                     /locus_tag="PK196_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON40550.1"
                     /translation="MTSLIDIDGDDASKYRLIGDRVLSYRFAVPHDERLYLGDVLKIT
                     DDVKGLTFFAKVNDLVHDCNFSDPKWDTRPHTEHFYGMGEDVFILVEATPLGYVGGDG
                     IFRKPRTIPAKFSRVERPEPRDFEFLQQVMGDIEVGVMKNGEGEFRDVRVALHAPVMR
                     QHMGVFATTGMGKSNFMKVFCASCMRARRFGLLIVDPHGEYVAGGRSSSGEATRGLLH
                     YQAGRDGLAVFSTREDDFIKKYHLNRLYLDHDDFSAPDLLLLYEHSTPQREVVEMLAD
                     RPGSDLIEFFLKTDFATFDAGTYRGPHAGIARDLKNFSLSTLSVMQRRIAGMMNRNRR
                     FFRKQGSSIPDILKELHENRVVLIDIPGMSEQSELFVLSIITRRIMRSHQEEETGGEK
                     EPGQVLITIEEAQRVLGSGYGSTRTFREAAMEGRKFGVGLCVVTQQPKNIDARVLAQI
                     NTFVVMGLSDRGDREIIAGSAKQDLSHMDTEIQTLERGEAVISTIGIPFPVSTRIHLY
                     EDYIEELNRRPAARAIDEGLEKTF"
     gene            complement(12567..13076)
                     /locus_tag="PK196_03655"
     CDS             complement(12567..13076)
                     /locus_tag="PK196_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HON40551.1"
                     /translation="MQIGNIKVRVTAITVAVFLFFIALIAVYVFISGDVHALLWALPA
                     AVMLLLIPMALNYMSQKQYASLVPVYEVEAKNARIREINLNRLGEPVRIKGVVERVYF
                     QFLNRPQYLVADRTGEISVKMFTSPTEDIHEGDLVEVLGTVVKRYILVGDAVINCVSI
                     RKVKKNQQS"
     gene            complement(13109..13981)
                     /locus_tag="PK196_03660"
     CDS             complement(13109..13981)
                     /locus_tag="PK196_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HON40552.1"
                     /translation="MVFHPPVAIVAKAGDTGKYKVGLPAWNMILRGIMSGAYIAMGGA
                     LATVCSTGVQATDVAMRVGTASPGFAQFMLGAVFPVGLIITVMTGAELFTGDAMLAPM
                     AAFIHKVSWASVLKLWLWVYIGNLIGSIIFAYIAAYGPFVSFDAMGAATLTPFGLRAI
                     AIATAKTNYLGGMALWSCFVKGIGCNWLVNLAILLGICADDAIGKIVGIWFPIFAFVA
                     SGFEHSVANMYFIPAGLFCTGLDPTKAVDTLNWVSMWTNNVIPVTLGNIVGGLLFVGV
                     LYWVAFRKEMAALK"
     gene            complement(14633..15868)
                     /locus_tag="PK196_03665"
     CDS             complement(14633..15868)
                     /locus_tag="PK196_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40553.1"
                     /translation="MNAIIPFALAFVLITIASLRYRLPPFLSLVGTSIVFGLLAGLPA
                     EMVITSITGGAGRIFATLGVVIFSGVGIAQVLRESGRIEEIVADIRGAVRRPLATAGT
                     AGYLLSVPLMCGITSFLILAPIISHLQTDGEASKALLYCAGIAGMISYVLLYPAPVVY
                     SMMATPGLFSGNPWKFNLLAIPVSLIILAGLFVVLRSRGIPPPAPPETTTTGWHPRAW
                     LPFLVILAALACGTLIPSLHALANVNLALLAGLFAALISVPGDVRERALARGTKNAGI
                     IIFDLAGAGAFGGVIAASAFSADVTALVTGYLPITLLPFVIAALVQAAQGSRVVTATV
                     TATILATIPAVQVINPFALVLMVSAGAFMFSYASDPFFWLLKQTTGDDFTAVVRNYTL
                     PLSVAGVVTLAAALLIQAL"
     gene            complement(15865..>16644)
                     /locus_tag="PK196_03670"
     CDS             complement(15865..>16644)
                     /locus_tag="PK196_03670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON40554.1"
                     /translation="AGSVDVIVTSPPYNIGKNYNSYHDRKPRQDYLDWMGDVAAEAAR
                     VLADDGSFFLNVGGKPTDPWIPFDVVQRFRPHFLLQNVIHWIKSIAIEKEDVGDYDRI
                     TGDIAVGHYQPVNSPHYLSQCHEHIFHLTKNGDVKIDKLAIGVAYQDKSNIGRWKAAK
                     RDLRDRGNTWFIPYPTIRSSLPHPTTFPVKLPEMCIRLHGCRPGLIVLDPFMGIGSTA
                     VAAISLGVDYIGFEIDPAYHEIAETRIEEECRRRGEPGNAP"
BASE COUNT         3400 a         4901 c         5133 g         3110 t
ORIGIN      
        1 gaagagtttc atagcggtga ttgcagcgcg gtttcttctt gacggccttg acaaccgcga
       61 cctccagatc ggcgagaaga gttttcacat acggggctac tctgcagact ccctcgacga
      121 gtgcatcatc gccgcggtgc aggcggaccg cgaaggagcg ctcgggatag tctgtgcccc
      181 tatccttgcg ccgatcgtgg aaaagatcgt cgattgtccg gtggggatta tcaagccgca
      241 ccagcagact ctgatcgagg cgatcgagac gattgcgagg aggatttagg gagactgtgg
      301 ggcccgtggc atagtaaccc cctttccggc tgactaccgg aacttccaac ccattggaga
      361 gaagaggctt cgtagacgaa caatccccac aaaaactcat ccggtaaagc cgccatgcga
      421 gaggtcacca gagatgagcc cccgggagcg gaggctgacg gcgctctgcg tcttctcccc
      481 ctccgccacc ttgcgaacga gggtgtaatg gcgctgccct caaccaggtt cgtgaccagc
      541 cgggatctgc atcgatctcg atcgccgcca gcacatgtca ttcggcgtcg tccatggtgg
      601 attgttcaac cacgatccat gagactgtgg cagcgggagt gtgaagcacc cgcacccgct
      661 ggtcctggtg ctgtctgtcg tgttaccgaa cagaccggtg aatatgccgg cgagatcgag
      721 tgtgtcaagg atctacccaa taatgtcccc ggagacgccg gataccgggg agtggattgg
      781 ctggtgaact cttgcgctgc agcgggaacg gcgcagggga gtccccgtga gaccgccggt
      841 catcacgcat ccgaacagag tttcagcccc actatcccgg caacgatcag gaggatgcag
      901 aggaggcgga gagcgctccg cgactcgtcg aagagcacca gccccgcaat gaccgttccc
      961 accgcgccga tacctgtcca gacggcgtag gctgtcccga gaggaagccc ggtgagcgat
     1021 cgcgagagaa ggtatatgct gcctcccatc aggacgaggg ttgccgccga cggccagact
     1081 ctggtgaaac cctctgtgta cttcaatccg atcgcccacc cggcctccag gatcccggca
     1141 aagaaaaggg ttatccacac tccgttctgc attataccta tatctggccg gaagaatgat
     1201 atatcttgcc tggattgggg ctgcacgccc ggtatacaga tgatggcagt cataaccacg
     1261 ttcgatccgg gcgggatggg gtgattgacc cggtgaaaaa ccgtgtcggg ggtgggagcg
     1321 ttcagcgaga ccgcaaagag cctccctgct ccccggcgcg gtatcgaggt ataaccgtca
     1381 ccccggggtg gggctgacgc tcgaggtgac cgatcgccct cctcccctcg acgggggcgt
     1441 tctgcctgtg ctcttcgtaa acttccaccg tcaaacgccc caacgaaagc cgtgcccggg
     1501 agagaataga ggttcaccgg aaaagaggcg gccaccccct ccacccccta tactcgtcac
     1561 ttgccagcgg cgccgccacg gagagggggg ccggatgaga tgcgatcact gctcgatctt
     1621 ctttatgtgc tcgatggcgc tctccgccac cttctcccgg tcctggagtt ccgcgacgtc
     1681 ccgtatcgcc tcgagcacgt ggacagagtc ttcaaggaag ctcagtatat cagcagggta
     1741 gagatcgatc ccgtattctt caaggagatg ggcgctgatc tggcggtggt caagccccat
     1801 ctcacgtagt tcgatgatcg tgagggtgaa cttgcgctcc gggcagccgc agtatggtgc
     1861 gttcttgcat ttgcagtcca taaaatcgcg aaagattgcc agcaggttct cctgcatccg
     1921 ccggtcgagg ttctcgaagt ttacaccccg tccgaccgcc tcgaggaaac cacccgagaa
     1981 gacatggtcg gggaggcgga accccgctgc ccgctccagt ttccgtgccg ctcgaaaacg
     2041 ggccttatct gcgatcacgc cgcttctcca ggctcctcgt caaactttgc aagcgatttc
     2101 atggcattgc tcatcgtctc gatctcgatc agccagtcgt caaagaggct caggctctct
     2161 gcgtcgtcct tgatgtactt cgcgcagcag gtggcgccgt cgatcacagc ggccccgatg
     2221 ttggccgcaa tctcaagcgc ctcatcgagg tttgtatcgc cgatcgcctt gcctttcttc
     2281 acaagcgtct ttatatccgt atcaaaccca ccgtcgagat acttccggca ggctgtgaag
     2341 agcacgatca ggctcaactg gagcgatcca acgagatcct cgagtctccc ctccggcagc
     2401 ccggtcatga cgagcacctc cacatcattg agtttttcta tggcctcctc ttttgttaac
     2461 cggccgttct ggtagaggcg gatgatcttt aagacagaga gtgtgagatc ctcggcgaaa
     2521 ccgtagagga tctgttcgcc ctccgagacc tcctcgccct ctgcgggctc aaaacttgcg
     2581 tcctcaagcc tcctgatcca gttgtcccac cgctccttgt tgtaaaaaat atagaagagt
     2641 ttcatcggtt ctgcttcttt agcagccgtt ttcttcgccg atttttttgc cataccagta
     2701 ccttcggagt gcctctgtta aaaaccattt gctcccctgc aggaagcaag agaaccatcn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna caaccaggca catgataggg
     2881 tgatgatagg gggtgaaccg gtggaagcgg tgatggtcag gtacggtgag gtctttctca
     2941 agagcgagcg ggtgaagcga cggtttgtct cggccatggc agagaacatc ggccttgcgc
     3001 tggaggcaga agggctcgcc caccggatcg agacccaccg cggacggata cttatatacg
     3061 gcgatgaccc cgcccggatc gccgagacgg ccgccagggt ctttggggtg gtgagcgcga
     3121 gcgtctgcac cgtgacgacg gccgatatag agggtataac gaccgcagcc ctcaaacgcg
     3181 cagagcgtcg cctccgtccc gggatgtcgt ttgctgtccg ggcacggcgg tctggcgtcc
     3241 cgggtttctc cagccaggaa cttgcggcgg cggtcgggtc ggccatcctc gaacggtttg
     3301 acggggtcag ggttgacctg acacaccccg attacgaggt ctctgtcgag gcaagagagt
     3361 tcggcggtct cgtctacgac gagaagatcc ccgggccggg tggactccct tacgggacac
     3421 agggggtggt catcaccctc ctctcggcgg ggatagactc gccggttgcg tcctggctga
     3481 tgatgcgccg ggggtgccgc atgctccaca tcaacatgca cggcggccgg tttggcgggg
     3541 ccgatgtcga gcgaaaagtc ctgcagaacc acgctcgcct ctcctcctgg gtgccgggcc
     3601 gcgccctcga ccttctggtt gtcgatatgg agcccttctt tgaggtcatc accgccttaa
     3661 aagagccgcg ttaccgctgc gtcctctgca aacggttcat gctccgggtg gcaggcatcc
     3721 ttgcagagga acggggggca cacgccatcg tcaccggcga caacctgggg caggtggcct
     3781 cccagacgct cgccaacatg gcggtgatat ccgccgcaac gaccctcccc gtgctccggc
     3841 cgctgatcgg gtttgacaag gatgaggttg tcgggcgcgc ccgcagcatc gggacgtttg
     3901 aggccaatcc aggggagatc gggtgtgcgg ccgccccccg ttacccctcg acggcagcgc
     3961 ccgcagagac catcgcccgg atcgaggagg agatcgatgc cgtggggctg gcagaacggg
     4021 cggcggcaag tgtgcgggtg atccgcgcaa aaaacggtgc ggtagaggag gcagcctcca
     4081 cctcagatga tacttaaccc gttctcacgc gcaatctcta taaaagcctc tgagagatgc
     4141 cggatctgtt tttccgtcag cccgaaggtg ttgaacttcc agacccgcgt ggagcccggg
     4201 ataacgccga taacacccct cttcttgagg tcgtttgaga ggaaatagcc cttcttcttg
     4261 tgctgctgcg caacgcggtc aaacgactcg cgtgtatcga tccgggtcag ggtgtgtctt
     4321 ctcgggtatt cggagagcac cttcgtcccc tcgatggaga gaaggctgtc gacgaccgcc
     4381 tgagagtgtg caacctccgt ctcccagtga ttcacccgct cctggacgtg ggggaacgag
     4441 gccatcatac cgacgacggt aacgcccatc agggtgcagc ccatcatctc gacctccttt
     4501 atgccgaacg tcctgccggt cagatcaccc ctcgcctctg tcgtccggaa caccttcccg
     4561 gcgtactcct ccgctgccgc gagaaccccc gatggtgccg gggcggccat gctcttgtgc
     4621 cctgagccca ccagaaaatc ggcgccgagc gccttcccgt cgacgggcat gatccccacg
     4681 gtgtaggcgc cgttgaccag caccggtatg tcatactggt gggcggcctt tgtgatcccc
     4741 ttgacgtcgt ggacgttccc gtactggtaa tcgacatggt cgatgaagag gagcggcggc
     4801 gtcctcgaaa actcccgact caccgcttct atcctctccg ccgcggcatc ggcggtgatc
     4861 ctgtttgagt cgtccttcgg gatctcacgc gggatcccgc cagcctcctc gatcgccagg
     4921 aactccgtgt agtgggcaag cgatgccagg atgaccgggt cgcccctctc cacaagactg
     4981 ctcgcgaccg cctggaaccc ccggcgcgct ccggggacca cccggacagc gtccatattc
     5041 agccaccggg caaggtcggc gtggaactgg gcgatcgggg gtttctggat ataatcgagc
     5101 ctgaatgggc tccggcagtt gtcacagacc gagtagccgt cggcgtatgc tatcaccgcc
     5161 ttcatggcgt ccgcggtaag ccgccccccg gcctggatgg ggtcgatgtt gatgtagatc
     5221 tcctccacat cgcgggcctc gatatcggag gcgcacctca cccgaccagc tcctcttcaa
     5281 ggattgcaac ctgccgtctt accttctcca gggttgttgc ggcgcgttcc cgctgcccct
     5341 cgtccagttc gtggtcggga gcggtctccc ggagcaggaa ccggatatcg gttaagagga
     5401 atagtgcctg aaaaactgca tcaacggccc gttttgacac aatatcacca tacaaatatc
     5461 agtgaggata tattagacta cgcgtattat ggcccccgaa cactttcgca ccgcgcagat
     5521 caggctttta tactctggcc gcagggagaa ttataaggca tgaagatcgt tcacctggca
     5581 gacacacacc ttggattatc ggcattcagc cgcatcgacc cggagaccgg gatgaacctg
     5641 cgtgaacaac tcatctacaa caacttcctc gccgccatcg accggataat cgccatccgc
     5701 cccgatgcgc tcgtccacgc cggcgacctc ttccaccagg tcaaaccaaa gacccgcgcc
     5761 tacacgacgg cgctcgacgc cctcgcccgg ctgcatgagg cagggattcc catgatcgtt
     5821 gtcgcgggca accacagcat ggccaggacg cgttacacgg cgtcgccgtt tgaggtgctg
     5881 gaacgcggcg gctaccgtgc agcagacctc tacgccgccc acaacaaccg ctaccggagg
     5941 gtcgaactcg gggatacggt ctttcacctc atcccgaaca tgatcgagcc cgcgggttat
     6001 cggagggcgt ttgaggagat cgagttctcc ggcggcacga atgtgcttgt cacccacggg
     6061 cttgcagcca tcctatccga ccggcggctt cacaccgtcg ccgagcacga acttgacgcg
     6121 accatcctct ccgacaggtt cgactacatc gcgctcggcc actaccacgg ccaggtccag
     6181 gtcgcggaga acgcctggta cagcggttcg cttgaatact gcaactacgg agagatcgat
     6241 gacaggaagg gcggactcct ggtgaatctt gccgccggcg atatcgagca tatcgacctt
     6301 ccgcagacac cgatgctcaa cctgggcagg atcaactccg acggtctgac ggccggggag
     6361 gttgtcgcgt ccatcctgga ggcggccgag gcccggtggg aagagatcga gggcgcgatc
     6421 tgccagatca cgctcgatgg gatacgccgc gagacgctcc gggccctcga ccagaaagcc
     6481 ctccaggacc tccggaaccg caccctggac ctgaggctgc aggccctgac cgttgatgac
     6541 ccataccacg tctttcacca ggactcgctt gcaggtctgg actacgtcac cgagttcgag
     6601 aagtttgtgg agagggagca ccttgcgcct gccgacgcag agtttgtgaa gaagaccggg
     6661 atggatgttc tccggtcggt catcgcacgc cacagagagg gagagagtgc tgctgaatag
     6721 gctctggatg aagaacttca agcggttccg cgaccaggag atcatcttcc aggacggtat
     6781 aaccgggatc atcgggaaca acggcgccgg gaagagcagc atagtcagcg gcatactgtt
     6841 tgcgctctac ggcctccagg ggacggggct tgacggcgat tacatcgtca gctcgtttgc
     6901 aggcccgcag gacgtctgcg aggtccgcct ggacttctct ctcagcggca acgattacac
     6961 agttctccgg aggttcaaac gccgcccatc ctcgaccctg catgaggcaa acctctacat
     7021 gaaccagaaa ctcctggcaa acagcgtcca gaaagtggca tcggagatcc agcggatcgt
     7081 cgggatgggg gcgggcgact tccgaaacac catctacgcc gggcagaagg aacttcttgc
     7141 cctgctggag agtcgtgcgg ggtcaaggaa agactggttc atgcaggttc tgggcatcga
     7201 ttacctcaag aaggacagca tggagtgtct caaagaactc atagatgccc gggaggggac
     7261 atgccgggaa ctctccggaa ggctccagga actggatccc gacgggattc gcgggcatct
     7321 tctggagttg cggactgcgg ttgccggtgc ggaggaagag gcggcaaacg cccggaccgc
     7381 ggagaccggc gcccgtgagg aacttgagag tgcccgccgc gactacgaga ggctgcttga
     7441 cctgcgggaa cgctgccggg aacttgaacg tgaggaggag aggctcacgg ccgatatcga
     7501 gcggctgcgt gccgagtgcc gggatatgga gaccgagatc gcggcccggc agcggctgca
     7561 ggccgacctt gacgaacttc aaccgatcgt agagcgctac gagccgctga aggcggaggt
     7621 cgccgcgctt gcagaggaga aggcgcacgc cgagcgcctg aacctcgaag cagccagtct
     7681 tgaggagcag atccgtgacc acgctgagcg catttcacgg cttgagaccg accttgcggc
     7741 gcttgcagag gacgaacgga cggttgccgc acttgaagag gatgtcagga gcgcccgggc
     7801 tcttcgcgag cgtattgcgg cgatgaaagc cctccagccg gattatgacg ccctccgtga
     7861 ggagatcgcc aggctggacg agcggtttgc tcacctcgag aagcgcgtcc tggagaaccg
     7921 ggccgatatc caggagctcg aagagaagga gagagatctc cgtgcgctcg aggaggagac
     7981 ggcagactac gagagccttc tcgcccgcct ggaggtttac caggaggcgc aggagcacgc
     8041 ccggcaggtg gagcggtctc tgcacgaggc cgaggccgca tccgcggcgg cgcgccggat
     8101 cgagagcgag atctccgatc tttcccggca gatcgctgaa ctcgagggtg tcgaggagcg
     8161 gatcgaggcg gcggagcgca gaaaggacga ccttacatcg cggatcagcg ccttcgccga
     8221 acgccgggag gcgatccgcc gggagatcca gaaggtggcc gggcacaggg aagagatcct
     8281 ggccgccggg cccgagggga cgtgccccat gtgccgccag agcctcggtg accactacca
     8341 ggacctcctc gcagacctgg catctaccgc cggatctctg gatagggccc ttgccgatct
     8401 cgatgaggag tatgccctgg cggtcgccgg gcagaagagg cttgccgccg aactgaagag
     8461 cctgtacgct cagcgggcgg ccctccagcg cctgaaagaa cagcacgctc tcgcagcatc
     8521 acgctacgag cagagcgtcg gcctgatgag acgctggcag gccgaggcgg atgagcaccg
     8581 ggccgagatc cgggctctcg gtctcgaggg ttacgacccg gcggcgcatg aatctctcag
     8641 ggaacggatc cgcgtcctcg ccgggaaacg ggcgaaggcc gataccctgc ggggcgagtg
     8701 cagccgtctc cctgtcctcc tggaggagag agagcggctc atcgccgatg ctgcggatca
     8761 cctggcccgg cgggatgagg ccgagacaag gctctacagg ctcgggttcg acccggaggc
     8821 tctgaagcgc ctggaagcgg aggcaggggc cctggaaccc gtatcccggg catataccga
     8881 ggcgcagacc agacttgcac ggcggccggc gctaaaggag gacctgaacg ccattaccgc
     8941 gaggcttgat ggcctccggg agcggctgcg gggtgtcagg ggcgaacttg ccggtctctc
     9001 gttcgacccg gagcggttcg aacgcctcag ccaggactac agccgcgctg aatcggcaca
     9061 ccggcgggcg ctcgaactcc gcgtccgtct tgaggaggtc cctcgactgg cggagagcct
     9121 cggtgccagg cgcgatctcc ttgcagagag ggaggccgaa ctgctccgcg tccgggctgc
     9181 tatcaatgaa ctcgggtttg acgggtcgat ggtaaaggcg gcagaggaga gggttgccgg
     9241 gtctgagcag aggcttgcgg ctgcacggga gcgtatgtat gccgccgccc tccggatacg
     9301 cagcctggag acggagatcg agcatgagac cgcaagactc tcccgcgcag aagaacttgt
     9361 ccggcagcgt gacgcgctca cggaggagat cgagcggttg aaactgaccc ggtcgctgac
     9421 cagggactac atcgactacc tcctccaggt tgtccggggc aggatcgagg aggaggcggg
     9481 ccgggtgctt gcagagatca ccgacgggcg ttacaacacg gtcatgctgg acgacgactt
     9541 cagcgtcctc gtccacgaca tgggtgaaga ctacccggcc gaccggttca gcggcgggga
     9601 gcaggacgat atcgccatcg ctctccgggt ggccctctcc cggttcatcg ccgaggtgaa
     9661 ccaggtgcac gactccacgt tcctgatctt tgatgagatc ttcgggagcc aggacgagga
     9721 gcggcgggca aacctcctcc gggcactccg gacgcaggag gcgtatttcc cacagatcat
     9781 actcatatcc catataagcg aggtccagga cgagttctcg acggcgctgc tcgtcgagat
     9841 gggcccggac cagataagtc aggtcagggt ggtcgactga tggacgcaga gaccgcctac
     9901 cgccaggcga tccggcgcgt ggcggcgagg atccgggagt gcgccccggc agacctttcc
     9961 acgcggtttg cagccgcagg cgggtttgat gcggcctcct acacccattt cgagcccggg
    10021 tttgaggggg ctgtctgcgc ggtggacggg agcaacgcca tcatccttga cgccgggagt
    10081 tttgccatcg ccgccatcag ggcctcggtc agcgcctttg cccgcggcag gcgcctctac
    10141 cgccgaacaa caccgctgca cgtcatcacg gtcaaccccg gcgccgggaa cgaggacttc
    10201 gacgagatct tctacgactg tttccgccgc cgcccggggg cgcggcttga ccacgacgac
    10261 cccgcccgga acgccgctgt catccgggac acgctggagc tctggtcggc gatggagatg
    10321 gccgcagaac tcgataccgg cgacctcctc gtcctggacg gcacgctcca ggtccggcac
    10381 gcaagccacg ccgaggtcat cgaggctctc ctgaacctct gcaacctgcg cggggttctc
    10441 atagccgccg tgacaaaacg gacatccctg acctggggcg gtgggcaccc gatcgtcccg
    10501 gcggcggaag ggcttgcgcg cggcgttggc gttgccgcac cctggtacct ctccgtctcc
    10561 ggcgcgagcg gcctgatcga tcgccaggag gcccgggcct ggaaacagcg gggggagcag
    10621 tacgttgcgc gtctccaccc gcgtgcagcg cgggcgttca aggttgagat cccgggccat
    10681 tacacccccg atatggtcga tgcggtcttc tccgggcttg cggcctactc cgacgacggg
    10741 agggtgacgg gctaccccta ccccctcctc gacgcccatc tcacaacaag gataacgcag
    10801 gacgcggtcg agcagatccg ccaggacatc atccagggcc tggacaggct cgggatgagc
    10861 cttgccgact acatcggcat atttggtgat taccatgacg agtttgatcg atattgacgg
    10921 cgatgacgcc tcgaagtacc ggctgatcgg agatcgggtg ctcagttacc ggtttgcagt
    10981 gccgcatgac gagaggctct atctcgggga tgtgttgaag atcaccgatg acgtcaaagg
    11041 gctgactttc tttgcaaagg tgaacgacct tgtccacgac tgcaacttct ccgacccgaa
    11101 atgggacaca cgcccccaca ccgagcattt ctacggtatg ggggaggatg tcttcatcct
    11161 ggtcgaggca acaccgctcg gctacgtcgg cggcgacggc atattccgca aaccaaggac
    11221 gataccggct aagttctccc gcgtggagcg gccggagccc cgcgattttg agtttctgca
    11281 acaggttatg ggggatatcg aggtcggggt gatgaagaac ggcgagggcg aatttagaga
    11341 tgtcagggtt gcgctccacg caccggtgat gcgccagcac atgggcgtct tcgcgaccac
    11401 cggcatgggg aagagcaact tcatgaaggt cttctgcgcc tcgtgcatgc gcgcccgcag
    11461 gttcgggctc ctgatagtcg acccgcacgg ggagtacgtg gcgggcggcc ggtcctcgag
    11521 cggcgaggct acccgggggc tcctccacta ccaggccggc agggatgggc ttgcggtctt
    11581 ctccaccaga gaggatgatt tcataaagaa gtaccaccta aaccggctct accttgacca
    11641 cgacgatttc agcgcgccgg acctgctcct gctctacgag cactcaacgc cccagcggga
    11701 ggttgtggag atgctcgcgg acaggccggg ttcggatctg atcgagttct tcctgaagac
    11761 cgattttgcg acgttcgatg ccgggacgta cagggggccg catgccggga ttgcacgcga
    11821 cctcaagaac ttctcgttga gcaccctctc ggtcatgcag cggcgtatcg cgggcatgat
    11881 gaaccggaac cggcgtttct tcagaaagca gggttcatcg atccccgata tcttaaaaga
    11941 actccacgag aacagggttg tcctgatcga catacccggt atgagcgagc agagcgagct
    12001 ctttgtcctc tcgatcataa cgcgcaggat aatgcggagc catcaggagg aggagaccgg
    12061 tggcgagaag gagccaggac aggtgctgat aaccatcgag gaggcgcagc gggtgctggg
    12121 ttcaggctac gggagcacaa ggacgttccg ggaggcggcg atggaggggc ggaagttcgg
    12181 cgtgggcctc tgtgtggtca cccagcagcc aaagaacatc gacgcccgcg tcctcgcgca
    12241 gataaacacg tttgttgtta tgggcctctc cgatcgcggc gatagagaga tcatagcagg
    12301 cagtgcaaaa caggacctct cccacatgga taccgagatc cagacccttg aaaggggaga
    12361 agcggtcatc agcaccatcg ggataccttt ccccgtgagc accaggatcc atctttacga
    12421 ggactatatc gaggaactca acaggagacc tgcggcacgg gcgatagacg agggtcttga
    12481 aaagacgttt taggatcaaa atgctgaatg tgagtgaggc tggaaatcac cctgtttcct
    12541 tagccccttc gctgaattga atgacatcag gattgctgat tcttcttgac tttccttatt
    12601 gagacgcagt ttatgactgc atcccccacc aggatgtagc gtttgacgac cgtcccgagc
    12661 acctcgacca gatccccttc gtgaatgtct tccgtcggtg aggtgaacat cttgaccgat
    12721 atctcacctg tccggtcggc tacaaggtac tgcggccggt tgaggaactg gaaataaacc
    12781 cgctcgacaa cgccctttat gcgcaccggt tcaccgagtc ggtttaaatt gatctcgcgg
    12841 atacgggcat tttttgcctc aacttcatac accggtacca gggatgcata ctgcttctgg
    12901 ctcatgtagt tgagcgccat agggatgaga agcagcatga ccgctgccgg aagtgcccac
    12961 agtagcgcat gtacatcccc gcttatgaag acatacacgg caatcagggc aataaaaaag
    13021 aggaagacgg ccaccgtgat ggccgtcacc ctcactttga tgtttccaat ctgcatcttt
    13081 actcaggtct actcaggtct actcatgtct acttaagggc tgccatttcc ttccggaaag
    13141 cgacccagta gaggacacca acgaagagca gaccaccgac tatgttgccg agcgtgactg
    13201 gaatcacgtt gttggtccac atgctcaccc agttgagcgt gtctacagcc tttgtggggt
    13261 caagacccgt gcagaagaga cccgccggga tgaagtacat gttagcaaca ctgtgctcga
    13321 aaccgctggc aacgaaggcg aagatcggga accagatacc aacgatcttg ccgatcgcat
    13381 cgtcggcgca tatacccagc aggatggcca ggttgacgag ccagttacaa ccaatccctt
    13441 tgacgaagca tgaccatagc gccatgccgc cgaggtaatt ggtctttgct gtcgctatgg
    13501 caattgctct taaaccaaat ggcgtgagcg ttgcggctcc catggcatcg aaagagacga
    13561 acggaccgta tgctgcaata tacgcaaaga taatcgatcc gatcaggttg ccaatataca
    13621 cccagagcca gagtttaagc acacttgccc agctgacctt gtggataaac gctgccatgg
    13681 gggcgagcat cgcgtcaccg gtgaagagtt ccgcaccggt catgaccgtg atgataagcc
    13741 ctacagggaa gactgcaccc agcataaact gagcgaaacc gggacttgca gtaccaaccc
    13801 gcattgcaac gtcggttgcc tgaacaccgg tcgaacagac tgtcgccagg gcaccaccca
    13861 tagcgatgta agcccctgac atgataccac gcaggatcat gttccaggcg ggcagcccaa
    13921 ccttatactt cccggtatca cccgcttttg ctacgatagc aactggagga tggaacacca
    13981 ttcttcatac acctctctga aacgtcctac ctatgccatc aggtaattca aatggatagg
    14041 tctaattgat cagtaattag aggatgctat taatacattt cgaaatgatt gcctcacttg
    14101 aggggtgtta tcagaatcgg cgctgaattg agatggttat ctctgtctca tgttaattac
    14161 catagttaaa catgaaagat cgatatccga tgttctggtc gttgtttcta accggcgtgg
    14221 actgcagatg attccagaga ggttttgaag aagtatgcag aagcaggcgc gcagatctgc
    14281 cctccttcat tcaggagacc atgacgggaa atggacgcac ggtaaaacgg ctttcgttct
    14341 ctgtttgcca ctatatgctc tggaaaacgg gtatcagcac ctcctgaacc ggagtgacgc
    14401 gcgggggtgg atgtcaggca cctccggcga tgcctcctct accgggatgg actggaggat
    14461 acgcaatccc ctgctctctg tctccaaggt gcttcccgga gggtgtacct cgcgttgact
    14521 cacctcaatt gcggatctgg aaaccctgcc gggctgcgag agcgagacaa cctgatgaaa
    14581 cgatcatatc tccggcactc agttctcgga aaaccgccgc gcggcggttg ggttatagag
    14641 cctggatcaa cagcgctgcc gcgagcgtga ccaccccggc taccgatagc gggagggtgt
    14701 agtttcgtac aaccgccgtg aaatcgtcgc ccgtcgtttg ctttagcagc cagaagaagg
    14761 gatcgctggc gtaggagaac ataaacgcgc ccgcggagac catgagcacc agggcaaacg
    14821 ggttgatcac ctggacggcc gggatggtgg caaggatggt cgcggtgacg gtggcggtga
    14881 ccacccttga accctgcgct gcctgcacca gggcggcgat gacgaagggg aggagggtta
    14941 tggggagata acccgtgaca agcgctgtaa cgtctgctga gaatgcgctt gcggcgatga
    15001 cgccgccgaa cgccccggca ccggcgaggt caaagatgat gatcccggcg ttctttgtcc
    15061 cccgtgcaag cgcccgctcc ctgacatcgc cgggaacgct gatgagggcg gcaaataggc
    15121 cggcaaggag agccaggttg acatttgcta gagcgtggag cgaggggatg agtgtgccac
    15181 atgcaagcgc ggccagtatg accaggaacg gcagccaggc gcgagggtgc cagccggtcg
    15241 tagttgtctc aggcggcgcc ggtggaggaa ttccccggga ccgcaggaca acaaagagac
    15301 cggcgaggat gatgagggag acggggatgg caagaaggtt gaacttccag ggattgccgg
    15361 agaagagccc tggcgtggcc atcatggagt ataccacggg ggcggggtag aggaggacgt
    15421 aggagatcat cccggcgatg ccagcgcagt agaggagggc ctttgaggcc tccccgtcgg
    15481 tttggaggtg tgatatgatt ggggcaagga tgagaaacga ggttatgccg cacataagcg
    15541 gcactgagag gaggtagccg gccgttcctg ccgtggcaag cggccgccga accgcgccgc
    15601 ggatatcggc gactatctct tctatgcggc cgctctcgcg gaggacctgc gctataccga
    15661 caccggagaa tatgaccacg ccaagggttg caaatatcct gccggccccg cccgtgatgg
    15721 acgtaatgac catctccgcc ggcagaccgg caaggagacc aaatacgatg gatgtcccga
    15781 cgagggagag aaacggcggg aggcggtacc tgagggatgc aatggttatc agaacaaagg
    15841 cgagggcaaa ggggataatg gcgttcatgg ggcgtttccg ggttctcctc tcctgcggca
    15901 ctcttcttcg atcctggtct ctgcgatctc gtggtatgcc gggtcgatct caaaccctat
    15961 gtaatccacg ccaagcgaga tcgctgcaac cgccgtgctg ccgatcccca tgaaggggtc
    16021 caggacgatc agccccggcc ggcagccatg gagccggatg cacatctccg gtagttttac
    16081 cgggaaggtt gtggggtggg ggagtgaaga acggatggtc ggatacggga tgaaccaggt
    16141 gttcccccgg tcgcggaggt cgcgtttcgc tgccttccag cggccgatgt tgctcttgtc
    16201 ctggtaagcc acgccgatag ccagtttgtc gatcttcaca tcgccattct ttgttagatg
    16261 aaagatgtgc tcgtggcact gactcaggta gtgcggactg ttcaccggct ggtagtggcc
    16321 gacggcgatg tccccggtga tcctgtcgta atcgcccaca tcctccttct cgatcgcgat
    16381 cgacttgatc cagtggatga cgttctggag cagaaagtgc ggccggaacc tctggacgac
    16441 gtcgaacggt atccaggggt ccgtgggttt accgccgacg ttgaggaaga acgaaccgtc
    16501 gtcggcaagg acgcgcgccg cctcggcagc aacatctccc atccagtcga ggtagtcctg
    16561 gcgcggtttt cggtcatgat acgagttgta gttcttcccg atgttgtagg gcggcgatgt
    16621 gactatgaca tcgacagagc ctgc
//
LOCUS       DAOSZX010000026        16437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2932_length_16437_cov_21.432548, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000026 DAOSZX010000000
VERSION     DAOSZX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16437
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_16437_cov_21.432548"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..526
                     /locus_tag="PK196_03675"
     CDS             317..526
                     /locus_tag="PK196_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40555.1"
                     /translation="MSQTRIKRYKPPISATAKPFRVVRRLQSNPTYTPLMTGPAVNAA
                     VLAALSATSAMSGLTLGVCMRLAGL"
     gene            666..1616
                     /locus_tag="PK196_03680"
     CDS             666..1616
                     /locus_tag="PK196_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON40556.1"
                     /translation="MGATDIIEVVDLEHTFGEVEAVRGISFAVKKGEIFSFLGPNGAG
                     KSTTINILITLLPLQKGRVRVAGHDVARDPRKVREVIGIVFQDEVLDRDLTVWETMEF
                     HGRIYGIPRDERRRRIDDLLAVVELDARRDERTKNLSGGMKRRLQIARGLLTRPEVLF
                     LDEPTLGLDPQTRMRVWDYIRQVNEAGTTIFMTTHYMEEADMLSDRISIIDHGRIVIS
                     GTPDELKNTLGEDVVYLETDDDERAREVLLGVEDVRSVTRSPRGLSVTISADGSHCLP
                     RIIDRVRGAGIGIVSVNLKKPTMDDVFVHYTGRELRDGGG"
     gene            1623..2459
                     /locus_tag="PK196_03685"
     CDS             1623..2459
                     /locus_tag="PK196_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40557.1"
                     /translation="MGWEFLTVYWREMIRFVRFRTQLFSSLLQPALWMGFFGIAMSSN
                     LDRFASAIPVPAGVVQVDYLTFMAAGVIAMTTLFTSLYGGMSLLYDKNWGLMPEMLAS
                     PMPRTHIMLGVSLSGMTKSFIQAGIIMVFGLVLGVRFFHGFSAARIAFSVIGVFAFLG
                     LFSLGFLLFSSNISMRLESPEGLQGIMTLISLPIFFVSNALYPVQAFPPFLQVLSAYN
                     PLTHLVTGIRYFALGNDFFAVGARYAITTGDVLTSFAYLAVFAAVMYLLARRAFERAV
                     VA"
     gene            2723..2902
                     /locus_tag="PK196_03690"
     CDS             2723..2902
                     /locus_tag="PK196_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HON40558.1"
                     /translation="MQTYRCSICGHIYDPKTGDVDAPPGTAFEDLPDDWRCPICLAEK
                     SKFSVFSSPLGRLGL"
     gene            3880..4332
                     /locus_tag="PK196_03695"
     CDS             3880..4332
                     /locus_tag="PK196_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HON40559.1"
                     /translation="MRHHGCRKNLSRRVEDYLEAILNVTLEKGYARTKDVARELDVSP
                     STVVEMFRKLGSMGLVDYRRYEGVVLLPAGREVAEAVKFRHDTLKEFLKVIHVPETIA
                     NSDACYMEHELHPTTIRQIRFLVEVLKSDPRICERIRQNTSRWEQQSV"
     gene            4329..4604
                     /locus_tag="PK196_03700"
     CDS             4329..4604
                     /locus_tag="PK196_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HON40560.1"
                     /translation="MTQVVAVIAVYLLVNLVSFITYYRDKRLAERSAWRIPEKRLLTL
                     AFFGPFGAFAAMRMFRHKTQKRVFRLVPLFLCLHLLLAAVLLLAGLC"
     gene            4850..5323
                     /locus_tag="PK196_03705"
     CDS             4850..5323
                     /locus_tag="PK196_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40561.1"
                     /translation="MIDEVCRVRQQTIPRIEHDTGYAFRSPETLPWILINPSASRLFS
                     GILAGFEEGALPVKANDLARLANTSTGIQALALIGDVTLVLKILPGRRVGSEELAALC
                     DRWSLCENLIGGGNRRRPTGETLEREKAALTLAVLGLIYTEGGDDALRPLVPLLN"
     gene            5438..5713
                     /locus_tag="PK196_03710"
     CDS             5438..5713
                     /locus_tag="PK196_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HON40562.1"
                     /translation="MSLLTGLLALILVIVIAFVLYRLVKSVTGLIINAVVGVILLWVI
                     NLVGLMGMAGRPDIPINIITVLVCAIGGVFGVIITLVLHLLGIPLSI"
     gene            5780..5959
                     /locus_tag="PK196_03715"
     CDS             5780..5959
                     /locus_tag="PK196_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40563.1"
                     /translation="MEDYGPGIIGEVHFSRQDGGYYVELYDTEGNPVGRTGLWRTEAK
                     AREAARRLAAKRGRA"
     gene            complement(5965..6915)
                     /locus_tag="PK196_03720"
     CDS             complement(5965..6915)
                     /locus_tag="PK196_03720"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HON40564.1"
                     /translation="MEPLQQPTVLILGAGAVGLSLAGKLHGVATACVACRQVHASAIV
                     ERGLVMEGVWGDRTVRGIRCVMGPEDLPTSIDFIIITAKGTDTRAICEEYAAVIRDRP
                     TATLQNGIGNEEIIAEYTDTVIGGTVTTNFSVLGPGHVRVLSESAPMRLGIWSGGADA
                     ARALDRLIALIRSASIPVEAEPDIRAGKWAKALLNIAVNPICALLRAPVGVAADPELR
                     QTVSSLIRETFAVAGAEGVKLPWATPDEYLGYLFGVQIPDFAAVYPSMYYDLQRGRRT
                     EIDLLNGYIAAAGERRGIETPYNRCITALVRSAEKQPGRA"
     gene            7029..7859
                     /locus_tag="PK196_03725"
     CDS             7029..7859
                     /locus_tag="PK196_03725"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HON40565.1"
                     /translation="MKYVTKANGSRQPFDRGKVQRTLQRMGVGMEDAERIAREIEEAV
                     PDGVKTSAILQMVRARARIVRPAVAYRTNLRRALALLRSKPEFEEFVRVLLREHGYQV
                     EAGCVLAGFCGEHEVDGIAQKDGTTVFVEVKHHVSPHRMTGLDEGRIARAIVEDLQEG
                     FRAGRCQVSIDGALLVCNTKLTDHAKRYSNCRGIGHIGWDYPEEQNLRSMIEGTQSYP
                     VTIVSGVSQSSIARLAAAGFVMAKQVAYGDASAIAHVSGVPQKDVLLVAGRARAILEQ
                     "
     gene            complement(7903..8853)
                     /locus_tag="PK196_03730"
     CDS             complement(7903..8853)
                     /locus_tag="PK196_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON40566.1"
                     /translation="MKLKAPGVRYIALLVVILIPSVTGYMALEAREIDVTILEIDGAP
                     EGIVYITDPHVQASNIDHVRAVIAGINRLEPTIVLIGGDFVTGDEEDFALQEVWSQLD
                     APTYAVLGNHDYRVGTNGTTGLERTLATRASATLTADGYDVSALNDGSADTAFAEELI
                     AALEENGVHVLRNEYLRLTAGDREIVIVGIDDGWAGMADPPSVPKTDAFTVYLVHEPS
                     CRADWDADLILAGHTHGGQFLFPIIKQLNDQGSIELAGLFDADGRTPTYISRGICSAD
                     LAGVDLRFNCQPEIVLINPTDEQLQALEDPVDLIHVARKT"
     gene            8949..9131
                     /locus_tag="PK196_03735"
     CDS             8949..9131
                     /locus_tag="PK196_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40567.1"
                     /translation="MELPIVHLEAGSRAESPGSPGGWILDGSCYRSMDGVLLVAVRKF
                     APASLGAPPPLRRENI"
     gene            9196..9525
                     /gene="eif1A"
                     /locus_tag="PK196_03740"
     CDS             9196..9525
                     /gene="eif1A"
                     /locus_tag="PK196_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HON40568.1"
                     /translation="MTDPAKRKPASGGNGDGGEEIIRVRLPNKRNREVFGSAELMLGA
                     NHIKVRCFDGVTRIGRIKGKIKKKVWIREGDVLIVVPWAFQDEKCDIVYRYTKPQVEW
                     LRKNGYL"
     gene            complement(9597..10337)
                     /locus_tag="PK196_03745"
     CDS             complement(9597..10337)
                     /locus_tag="PK196_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40569.1"
                     /translation="MNDRAVSEAIGFIIILGIVMSGIGLVTLYGYPVLVKEQSNTDVR
                     NMERAMIVIQNDMKSLCFKNVPYKETALQVSGGTLEVIGGDDYGAKFTISNTTSQWEF
                     SPGALVYRSDRGTEVITLENGAVITRQEDAAGSAMLAEPRWFYDETTKTFAVYIMKIT
                     TDEAMARSGVATVRMSLEGVVTNVSSSPGGEEITVIYESDTSNDNSVAWGNYLTGSLG
                     MERSDDGYTLQGVTRLVVKEYWIRIHGI"
     gene            complement(10372..10809)
                     /locus_tag="PK196_03750"
     CDS             complement(10372..10809)
                     /locus_tag="PK196_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40570.1"
                     /translation="MNEDGVSRLTEYIIVTGVLLVLMIVMMFTVNSVFMQGPADKLRY
                     HAFVDIGNGISTRIVDLYVISPANGTIETKFDIPDDVAGADYFVEASGKGSSQMILVH
                     GGNVQNQVAIAGIGATMAVTCNTTGAGWNWIQYNSNAFEIRMT"
     gene            complement(10814..11200)
                     /locus_tag="PK196_03755"
     CDS             complement(10814..11200)
                     /locus_tag="PK196_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40571.1"
                     /translation="MACMNEDGQWIVLMGLLVAVGLFFLALIINQSALVGQTTVEGVL
                     EFPKNDIRDLREAIFDYVDKYGNTGGSAANNVRDDIIAISLDRKNAVVDFTVKDPVDI
                     SGQRAYPVEIHYNNGVTVYDETVYHY"
     gene            complement(11188..11691)
                     /locus_tag="PK196_03760"
     CDS             complement(11188..11691)
                     /locus_tag="PK196_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40572.1"
                     /translation="MVNDSGQLYTMEGIAAGLVMLLTAYLILSTTSIYTPGDTHIPDM
                     QLEQLGSDVLAMMDTPDSDGGENHLVNLINRSDRDGFNSTFLKYCNARTNGDPDTIHM
                     SASVAYRDGDRIEEHQLVKPDETWTGRESAVRVTRWVQLDQQSTRGDPYGSRAQAVLV
                     EVLLWRA"
     gene            complement(11685..12713)
                     /locus_tag="PK196_03765"
     CDS             complement(11685..12713)
                     /locus_tag="PK196_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40573.1"
                     /translation="MLRNDEGLFSIDFLVGFTIFMVALIAVASMVPGLLAGLQSSGID
                     YDAVAYRTGVILAEDPGWPVDPPWEAYDVNHSGEIERTGLAVSKDAPNILLSTKIEKF
                     FDTSFFDPEDYRSKAIFGDITYSYNITLRSLDGRYNHAVGQETPEGYGYIRRVVKIKE
                     PSVAVINGSFKDRYTISPDDNSTTRTFIVRFDFNRLINLSIDPAYRIDPRTEPVNITI
                     TDFEKFLNGTASEATLKSVTFRRGSSSIPFPYDEDDTDYYTLRIDNNLCPPEPGVGVS
                     SNISLVLKPAATSLFELGPTSILDVEFNFEDKPPDTNITGIHYYDYNPTNVTRPDLKP
                     AVLEVAIW"
     gene            complement(12713..13276)
                     /locus_tag="PK196_03770"
     CDS             complement(12713..13276)
                     /locus_tag="PK196_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40574.1"
                     /translation="MRFVGRVLALFAAVCIFCTASGCLDASTTPSEGGGITAVDLPEP
                     EGLTLAEALAELDLLGTEGGLNVTGASLHQVIGNRVDLEGLASSWTLGLLDAEGGRWL
                     TLGAAGWQEVSLPAPIPEEELNMTELLSPEEIFAMHKDGIRAAMDRLNTSTVDIVLAE
                     GVYTITTRSDAGMETLAFRADTGEVVV"
     gene            13526..14167
                     /locus_tag="PK196_03775"
     CDS             13526..14167
                     /locus_tag="PK196_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HON40575.1"
                     /translation="MCNNSDPGTPGEARGRFAEAVAFHGHVCPGLAIGYRAAEIALSR
                     LSSGRSRDEELVTITENDACGVDAIQVLTGCTAGKGNLLFRDHGKQAFTFINRKTGAA
                     IRVAANPSFDIESLDPEFAPLRKRVMQGEATGAERADFQEHVNRVVDAILEAPDDDLF
                     IIRKVDQTIPEPARIFRSVPCAKCGEMTAESRIRVEDGKFLCYACSENYSRCL"
     gene            complement(14183..15055)
                     /gene="hisG"
                     /locus_tag="PK196_03780"
     CDS             complement(14183..15055)
                     /gene="hisG"
                     /locus_tag="PK196_03780"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HON40576.1"
                     /translation="MITIALPKGSLEAQTLQVFKEADLEVRRTDRDYNPRIDDPRIGK
                     VKILRPQEIPLYVQMGYFDLGISGVDWVEESGADVVEVANLSYSKTGEGNVKIVVAVH
                     RDEPIEDVTAIRPGSRVTTEYPRITKRFFDDLGIPVRLFPSYGASEAKVPDLMDVVVD
                     LTETGSTLRKNGLKIVGEIMESHTALLANRDSLRDPHKHREIDEIATLLMGVIEARRQ
                     VLLTMNVPAAALERVIEVLPAMKRPTLSRLHGIDYFSIQTVVQKGLVNGLIPALKAAG
                     AEDILEIPITKIVR"
     gene            complement(15105..16370)
                     /locus_tag="PK196_03785"
     CDS             complement(15105..16370)
                     /locus_tag="PK196_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HON40577.1"
                     /translation="MVQIYRFAAIRPARRYSEEIPSVPYDVVTADEARECIEKNPLSF
                     LRVSRPDAELPEIPPNDDRVYQRAREVFESMLADGLMERDPEPGMYVYRAVQDGEEFV
                     GLVCCVPTEDYETGKIRRHELTRYDKEEDRTRHIDAVGANTGLVFLLYRDRQGIYPHL
                     HSLIEGVRPDGSTTTASGVLHQIYRITDDAALSHLEDLFSAVPETYIADGHHRAKSAV
                     NVAALRRREGRFTEEAGRFMVVLFPHDRVRIHGYSRLVTDLGRYATPREFLEALRESG
                     WTVRPCGSLDTEGYQIPPLEPDAGGGHVMHIYLDGAWYEISRPIADPTNLIGSLDVSV
                     LQKDILEGMLGISDPRADTRLHYMGGAKPLRELERLVDDGRYALAVAMQPVPIETVLA
                     IADAGGVMPPKSTWFEPKLLSGLVVHTID"
BASE COUNT         3253 a         4708 c         4876 g         3600 t
ORIGIN      
        1 tgattgtcac agaaaaggtt tatagaagag cgttgacttc tcatgcgtgg ataccaaaat
       61 cggtatgccg caatgtgcgg gggtccccca tccacggttg aggcccggat gatggagccc
      121 cgcaagcccg gaggctattg aggtgtacgc gttctgcgta cttaaacgtg tgcttctcct
      181 gcctccgggt actgaggcca tcaaaccagt gaggctgttg gtttgatggc ctccttttgg
      241 agatgctggt aaaccacaac cgttatctac aggagagtcg atcgagagtt gtgggttgtt
      301 cacccggtac agggaggtgt ctcaaacgag gatcaaacgt tataaacccc cgatctcggc
      361 gaccgcaaag ccgttcaggg ttgtgcggcg tttgcagtcc aacccgacct acaccccgct
      421 catgacgggt ccggcggtga acgccgccgt gcttgctgcc ctctcggcta catcggccat
      481 gtcggggttg acgctgggtg tctgcatgcg tcttgcaggg ctctgaaggc aaccgattgt
      541 acactttttt gatggtttct gccccccatc gattctttgc agcgcgctcc tccttccggc
      601 agggggaaaa gggatatcat gctgcgggag gagatttact gttacgcgcg actggatggt
      661 gaccgatggg agcgaccgac atcatcgagg tggtggacct tgaacatacg tttggcgagg
      721 ttgaggctgt gcgggggatc agtttcgcgg tcaaaaaagg tgagatcttc tcattcctcg
      781 gtccaaatgg cgcggggaag agtactacca tcaacatact gataacgctt ctccctctcc
      841 agaagggcag ggtgcgggtc gccggtcacg acgtcgcccg ggatccccgg aaggtcaggg
      901 aggttatcgg gatcgtcttc caggacgagg tgctcgaccg cgatctcacg gtctgggaga
      961 cgatggagtt tcacggccgg atctacggca tcccccgcga tgaacggcgt aggcggatcg
     1021 acgaccttct tgcggtcgtg gaactcgatg ccagacggga tgagcggaca aagaacctct
     1081 ctggtggtat gaaacgccgc ctccagatcg cccgcggcct cctgacccgg ccggaggtgc
     1141 tcttcctgga cgaaccgacg ctggggctcg atccccagac aaggatgcgg gtctgggact
     1201 acatacggca ggtgaatgaa gccgggacga cgatattcat gacaacgcac tacatggagg
     1261 aggctgatat gctctcagac aggatcagca tcattgatca tggcaggatc gtcatctccg
     1321 gcacgccgga tgaactgaag aataccctgg gagaggatgt cgtctacctc gagacagacg
     1381 acgatgagag ggctcgtgaa gttcttctgg gggttgaaga tgtccggtcg gtcaccaggt
     1441 caccccgcgg cctctcggtc acgatctcgg ccgacgggag ccactgcctc ccgcggatca
     1501 tcgatcgggt gcggggggcc ggtatcggga tcgtgagcgt gaacctgaaa aaaccgacga
     1561 tggatgatgt ctttgttcac tacacaggaa gggaactgcg cgatggagga gggtgagcgg
     1621 atatggggtg ggagtttctt accgtctact ggcgggagat gatccggttt gtccggttca
     1681 ggacgcagct cttctcgtcg ctcctccagc cggcgctctg gatgggtttt ttcgggatcg
     1741 cgatgtcttc aaacctcgat cggttcgcat ccgccatacc agtcccggcc ggggttgtcc
     1801 aggtcgatta tctcaccttc atggcagcag gtgtgatcgc gatgacgaca ctctttacga
     1861 gcctctatgg aggaatgagc ctcctgtacg ataagaactg ggggctgatg ccggagatgc
     1921 ttgcaagccc catgccaagg acgcacatca tgctgggtgt aagcctatcg gggatgacaa
     1981 agtcgttcat ccaggcgggt atcatcatgg ttttcgggct cgttttgggt gtccggttct
     2041 tccacgggtt ttcggctgcc aggatagcct tttcggtcat tggcgtgttt gcgttcctcg
     2101 ggttgttctc acttggtttt cttctcttct cctccaacat atcgatgcgg ctcgagagtc
     2161 ctgagggcct gcaggggatc atgacgctca tctcactgcc gatcttcttc gtctcaaacg
     2221 ccctctaccc ggtccaggcg ttcccgccat ttttgcaggt tctctcggcc tacaacccgc
     2281 tgacccatct cgtcaccggg atccgctact ttgctctggg aaacgatttc tttgccgttg
     2341 gtgctcgcta cgccatcaca acaggtgacg tcctcacatc ttttgcgtac cttgcggtct
     2401 ttgcagcagt gatgtacctc cttgcccgaa gggcgtttga gagggcggtt gttgcgtgag
     2461 gctccctcct ccaggcaatc gccgcgttcc catcccggtt tatcccggac tgtctcaatc
     2521 gcgttatgag aacccctgga cagaaccacg ttaagcggtg atcagggata tccgtctgct
     2581 gaagatgacc gggaggcggg ggctacgctt gagagtctct gttatactgg cattcccatt
     2641 cgcggttcga tacctggttt atggaggctc gtgtagttgg ttttaagtct gcggccggcc
     2701 cacttccaag ttgatgacaa ccctgcagac atatcgttgc tcgatatgcg gccatatcta
     2761 cgatccgaag accggggatg tggatgcgcc gcctggaacc gcgtttgaag acctccccga
     2821 tgattggcgc tgtccgatct gccttgcaga gaagagtaag ttcagcgtat tctcgtcgcc
     2881 actcgggcgg ctcgggctct gagcggtcag gccgcttgca caccattcat tgcctggcgg
     2941 gcaggagcat gtttcctcca ggcgtgtccg ccagaacagg ctgacccctg gcttctccag
     3001 ctgcactcat tttcaggagt tttatgtaaa accaaactca tttcgggcga aaatcatccc
     3061 tgccgaacgc cttaccaggt atacatgcaa gaggtgaaca tgaagttgaa aggcaggggt
     3121 gtcaaggaga ggtatttgag atgaatagcg gcgcttcgat ccgcggtgcc cgcaacccgg
     3181 aggaggtcca ggagtttatc cgttttctct ttggctctga tatggagcgt caggaggaag
     3241 aaccgcgttc aggtaacggg ataaccagtg taacgcccca atccgtagaa agcggcggat
     3301 catcatccta taggcgttcc ccctctcctt tttcgccacg aaggtcttca tgaggccgtg
     3361 cttcataccc ggtggtatgt gggggtgcgg ggtggcaggt gaactgcaca gaatgcatgc
     3421 gctgctgcat acccctgatc ggatcgtgtt ttgtttggtg ctattcccat gccggtttcg
     3481 gaaaagacca ttcaacccaa gaaagttcgg atgcgattga ttacgcccaa accgcctacc
     3541 ttttatttga agaacagatt ggaccggtca ctctgttgat ggggacgtcg gagcgaaaaa
     3601 atggtgacac gacgcgtggc gatcgggcgg gtaaccactc cagcgtctct cttgagttcg
     3661 cacgccccga ggcgttcctt tcacccttcc actacctgga agttgcttga agcaaaaaca
     3721 agtgagaata acaatcatat caccacacga gaaactatcc ggtatcgaag gtgaacctct
     3781 ctagaacagg tgccagagat tgaaagaccc cgcgccgacc cggcctctcg atcgttggtc
     3841 gtttaagtca gactctaaat ttgccgtatg agacaccata tgagacacca tggatgtaga
     3901 aaaaacctga gcaggagagt cgaggattac cttgaggcca tcctcaacgt cacgctggag
     3961 aagggttacg cccgtacaaa agacgtggcg cgcgagttgg atgtcagccc atcaactgtt
     4021 gtggagatgt ttcgaaagct tggcagtatg ggtcttgtcg attaccgccg ttacgagggt
     4081 gtggttctct tgcctgctgg gcgtgaggtc gcggaggccg tcaagtttcg tcacgatact
     4141 ttaaaggagt ttcttaaggt catccatgtc ccggagacga tagccaacag cgatgcctgc
     4201 tacatggagc atgaactcca cccaacgacg atccggcaga tacgatttct tgtagaggta
     4261 ctcaaatcgg atccgcggat ctgtgaacgt atacggcaga acacatcccg gtgggagcaa
     4321 cagtctgtat gacccaggtt gtggcagtga tcgcggtcta cctcctcgta aacctggttt
     4381 cattcatcac atactaccgc gacaagcgcc tggctgaacg ctcggcatgg aggatccctg
     4441 agaaacggct gctcactctt gcgttttttg ggccgtttgg cgccttcgcc gcgatgagga
     4501 tgttccggca caagacgcag aagcgagtct tccggctcgt ccccctcttc ctatgcctgc
     4561 accttctgct ggctgccgtg ctgctccttg caggtctctg ctgatacacc cctccagcat
     4621 tagaatgcgt ttaacatagc agcaaagtta aaatcaatat ctccaccaca ctacccccat
     4681 ggtctatgag tatggaccgc cttcctcctc tctggaagag gttcttttcg cgcttgagga
     4741 ttggctcaca cgccgttacc gccttgagta cgtccctgcg caccgtcgtt cagcccggca
     4801 gtccaggagg ctccggcgta tacgggggtg gagaagggcg accgtctgca tgatagatga
     4861 ggtttgccgg gtgaggcagc agaccattcc ccgcatcgag catgatacag ggtatgcatt
     4921 caggtctccg gagacgctgc cctggatcct tatcaatccc tctgcctccc gcctattttc
     4981 gggtatcctc gccgggttcg aggaaggtgc tctgccggta aaggcaaacg accttgccag
     5041 gcttgcaaac acctcgaccg gcatccaggc cctcgccctc ataggggatg tcaccctcgt
     5101 gttgaagatc ttgccgggga ggagggtggg gtcggaggaa ctcgcggcgc tctgcgaccg
     5161 ctggtctctc tgcgagaacc tgattggcgg cgggaatcgc cgtcgcccga ccggggagac
     5221 cctcgagcgg gagaaggcag ccctcaccct ggccgtcctt ggcctcatct acacggaggg
     5281 tggtgatgat gcgttgcggc cgttggtgcc gctgcttaac tgattgggtg ccgtgctgtc
     5341 cacttaatct ccgttgccgg cggatccagc atccgccagg atgtcctcct cacccccgca
     5401 ggaaggtttc aatatccctg gcgtcgtaga ggcaagcatg agcttactca ccgggctcct
     5461 cgcccttata ctggtgatcg ttattgcgtt tgtcctctac aggctggtaa agagtgtgac
     5521 ggggctgatc ataaacgccg tcgttggcgt gatcctgctc tgggttatca atcttgtcgg
     5581 cctgatgggc atggctggca ggcctgatat cccgatcaac atcatcaccg tcctggtctg
     5641 tgctataggc ggagtatttg gcgtcatcat tacgctggtg cttcacctcc tcgggatccc
     5701 gctctccatc tgaggcggcg cggtatatag gaagggtgtg cggaaccatc ataacacccc
     5761 ggtgataaga tccccgggta tggaggatta cggccccggc atcatcggtg aggtccactt
     5821 ctcccgccag gacggcggct actacgtgga actctacgac accgagggca accccgttgg
     5881 caggacaggt ctctggagga cggaggctaa ggcaagagag gcagcccggc gcctcgcggc
     5941 aaaacgtggc agggcgtgac tgtatcatgc cctcccgggc tgtttttcgg cggagcggac
     6001 gagcgccgtg atgcagcggt tgtaaggcgt ctctatacca cgcctctccc ctgcggccgc
     6061 gatatacccg ttgaggaggt cgatctctgt cctgcggccc cgctggaggt cgtagtacat
     6121 ggaggggtag accgcggcga agtcgggaat ctgcacaccg aacaggtatc cgaggtattc
     6181 atcgggtgtt gcccagggga gtttcacccc ttcagcccca gcgacggcaa aggtctcccg
     6241 gatcaggctg gagacggtct gccggagttc ggggtcggcg gcaaccccta caggggcgcg
     6301 gagaagggca cagatggggt ttaccgcgat gttgaggaga gcttttgccc acttgcctgc
     6361 acggatgtcg ggctcagcct cgaccgggat ggaagcggat cggatcaggg cgatcaggcg
     6421 gtcgagagcg cgggcggcgt cggcgccgcc ggaccagatt ccgaggcgca tgggagcgct
     6481 ctcgctcagg acgcggacat gcccgggccc gagcaccgag aagttcgtcg ttaccgttcc
     6541 cccgatgacg gtgtcggtat actccgcaat gatctcttcg tttccgatgc cgttctggag
     6601 ggttgccgtc ggtctgtcac ggatcaccgc ggcatactcc tcgcagatcg cccgggtgtc
     6661 agtgcccttc gcggttatga tgataaagtc aatcgaagtt gggagatcct ccggccccat
     6721 gacacagcgg ataccccgga cggtgcggtc tccccagacc ccctccatca ccagcccccg
     6781 ctccacgatc gcgctggcat ggacctggcg gcaggccacg caggccgtcg cgacgccgtg
     6841 gagtttcccg gcgagcgaga gcccgaccgc ccctgcgccc aggatcagaa cggtgggctg
     6901 ctgtagcggt tccatctgcc tacagttata tcacgggaac tgataagggt gcgccatcgt
     6961 tgtggcggtc gccgggaaaa acctttacca aacgtttttt cacccggcgt gatgagataa
     7021 cttcccttat gaaatacgtc acgaaggcca acggcagccg tcagcctttc gatcggggga
     7081 aggtgcagcg gacgctccag aggatgggtg tggggatgga ggatgccgaa cggatcgccc
     7141 gcgagattga agaggctgtg cctgacggcg tgaaaacatc cgcgatcctc cagatggtcc
     7201 gtgcccgcgc gcgcatcgtc cggccggccg tcgcctaccg gacaaacctg aggcgagcgc
     7261 tcgcgcttct tcgctctaaa ccggagtttg aggagtttgt gagggttctc ctccgggagc
     7321 atggctacca ggtggaggcc ggatgcgtcc tcgccgggtt ctgcggtgag cacgaggttg
     7381 acggcatcgc gcagaaagac ggcaccaccg tctttgtcga ggtaaaacac cacgtgagtc
     7441 cccatcggat gaccggcctc gacgaaggcc ggatagcccg ggcgatagtc gaggatctgc
     7501 aggagggatt ccgggcgggg cggtgccagg tctcgatcga cggcgccctg ctggtctgta
     7561 acaccaaact caccgatcat gcgaaacgct actcaaactg ccgcggcata gggcatatcg
     7621 gctgggacta tccggaggag cagaacctga gatcgatgat cgaggggacg cagtcctacc
     7681 ccgtaacgat cgttagcggg gtgagtcagt caagcatcgc cagactggct gccgccgggt
     7741 tcgtcatggc aaaacaggtt gcctacggtg atgcctcagc catagcccac gtttcgggag
     7801 ttccccagaa ggacgtgctg cttgttgccg gccgggcgcg ggcgatactc gagcagtagg
     7861 agagggttgg cggatgacag tttttcaggg cggagatgcc tctcacgtct tcctcgcaac
     7921 gtggatgaga tctacgggat cttcgagggc ctggagttgt tcgtcggtcg ggttgatcag
     7981 gacgatctct ggctggcagt tgaatcggag atcgactcct gcaaggtctg cactgcatat
     8041 cccgcgtgag atgtaggtcg gtgttcggcc atcggcgtca aagagcccgg caagttcgat
     8101 gctaccctgg tcgttcaact gcttgatgat cggaaagaga aactggccgc cgtgggtgtg
     8161 cccggcgagg atgaggtcgg cgtcccagtc ggccctgcag gagggttcat ggaccaggta
     8221 gactgtgaaa gcgtcagtct tcggcaccga agggggatcg gccatcccgg cccagccgtc
     8281 gtcgatcccg acgatgacga tctcccggtc accggcggtc agccggagat actcgttacg
     8341 gaggacatgg acaccgttct cctcgagggc cgctatgagt tcttcggcaa acgcggtatc
     8401 ggcggaaccg tcgttcaagg ccgagacgtc gtagccgtcg gcggtgagcg tggctgatgc
     8461 cctggtggca agcgtccgct cgaggcctgt cgtgccgttt gtccccaccc ggtaatcatg
     8521 gttcccgagc acggcgtagg tcggtgcatc gagttgactc catacctcct gtaacgcgaa
     8581 gtcctcttca tcaccggtga cgaagtcgcc gccgatcagg acaatggtgg gttccagacg
     8641 gttgatcccg gcgatcaccg cccggacgtg atcgatgttt gaggcctgga cgtgggggtc
     8701 ggttatatag acgatgcctt cgggcgcgcc gtcgatctcg aggatggtaa catcgatctc
     8761 ccgcgcctcc agggccatgt agccggtcac ggacggaatc aggataacca caagtaatgc
     8821 tatataccgt acgccaggcg ccttcaactt cactgcggta tgtttttgat ctcctggaaa
     8881 tatataagat ccgaattgag cggcgggggt atgctggagg ccatcagccc ctggcagcag
     8941 ggatctccat ggaactgcca atcgttcacc tggaggccgg cagccgtgct gaatcgcccg
     9001 ggtctccagg tggctggatt ctggatggtt catgttaccg cagcatggac ggcgtcctgc
     9061 tggttgcagt gcgcaagttc gcaccggcgt ccctgggagc gcccccgcct cttcgccggg
     9121 agaatatttg ataaaccagc agttgtataa actggagatg agcagactct ctggaaaaac
     9181 gttggggttg gtgaactgac agatcctgcg aaacgcaaac cggcgtccgg tgggaatggg
     9241 gacggcgggg aggagatcat acgggtgcgc ctgccgaaca agaggaaccg ggaggtcttc
     9301 ggcagcgctg aactgatgct tggtgcaaac catatcaagg tccgctgttt cgacggtgtg
     9361 acgcgcatcg ggcggatcaa gggtaagatc aagaagaagg tctggatccg ggaaggcgac
     9421 gtcctgatcg ttgttccctg ggctttccag gacgagaaat gcgatatagt ctaccggtac
     9481 acaaagcccc aggtagagtg gcttcgaaag aacgggtatc tctgatacac taattttttg
     9541 aagggggagg aaagtctctt ttccgcagtg gcgggaacca gggcgtaaaa aaatggttat
     9601 atgccgtgga tcctgatcca gtattctttt acgacgagtc tggttacccc ctggagggta
     9661 tatccatcat ccgagcgttc catacccaga ctcccggtca ggtagttgcc ccaggccacg
     9721 ctgttgtcgt tgcttgtatc gctctcatat atcacagtga tctcttcgcc gccaggtgac
     9781 gatgagacgt tggtaaccac gccttccagg ctcatcctga ccgtagccac accggatctt
     9841 gccatcgcct cgtcggtggt gatcttcatg atgtatacgg caaacgtctt cgtggtttca
     9901 tcatagaacc agcgtggctc tgcaagcatc gccgaacctg ctgcatcctc ctggcgtgtt
     9961 atcaccgcgc cgttctccag ggtgatcacc tcggtgccgc ggtcggagcg gtagacaagg
    10021 gcgccggggg agaactccca ctggcttgtg gtgtttgata tggtgaattt agccccataa
    10081 tcgtctccac ctatcacctc cagcgtcccg ccgctcacct ggagcgcggt ctccttgtag
    10141 ggaacgttct tgaagcagag actcttcatg tcgttctgga tgacgatcat cgcccgctcc
    10201 atgttcctga catcggtgtt gctctgctcc tttacaagca ccgggtagcc gtagagggtg
    10261 accagaccga tgccgctcat gacgatcccg aggatgatga tgaacccgat cgcttcagat
    10321 accgccctat cattcatctt cacggttctt ttcctccaaa aaagccctgt tttacgtcat
    10381 tctaatttca aatgcatttg agttgtactg gatccagttc catccagcac cggtggtgtt
    10441 acaggtcacg gccatggtcg ccccgatgcc ggcgatggca acctggttct ggacgttgcc
    10501 gccatgcacc aggatcatct ggctcgaccc tttgccgctg gcctccacaa agtagtccgc
    10561 cccggcaacg tcatcgggga tgtcgaactt tgtctcgatc gtcccgttgg cgggtgagat
    10621 cacgtagaga tccacgatcc gggtgctgat cccgttcccg atatccacga aagcgtggta
    10681 gcggagctta tccgccggcc cctgcatgaa gacgctgttg acggtaaaca tcatgacaat
    10741 catcagcacg agcagcacgc cggtgacgat gatgtactcc gtcagccggc tgacgccgtc
    10801 ctcattcata gactcagtaa tggtaaacgg tctcatcata caccgtcacc ccgttgttgt
    10861 agtgaatctc cacggggtat gcccgttgcc cggagatatc aaccggatcc ttaacagtga
    10921 aatcgaccac ggcattcttc cggtcgagcg atatcgcgat gatgtcgtcg cgaacgttgt
    10981 tggcagcaga accgccggtg ttgccgtatt tgtcaacata gtcgaatatg gcctcccgca
    11041 ggtcccggat atcgttcttc gggaactcga gcacaccttc caccgttgtc tgcccgacga
    11101 gcgccgactg gttgatgatc agcgccagga agaagagccc cacggcgacc aggagcccca
    11161 tcagcacgat ccactgcccg tcctcgttca tgcacgccat agcagcacct ccacaaggac
    11221 tgcctgcgcc cgcgacccgt aggggtcgcc ccgcgtggac tgttgatcca gttgcaccca
    11281 ccgggtcaca cgcacggcgg attccctgcc ggtccaggtc tcgtcgggtt taacgagttg
    11341 atgttcttcg atccgatccc cgtctcggta tgcaacgctc gcgctcatgt ggatggtatc
    11401 gggatctccg ttcgttctcg cgttgcagta tttcaggaat gtgctattga acccgtccct
    11461 gtcacttctg ttgataaggt tgacaagatg gttctcaccc ccatcactat ccggcgtatc
    11521 catcatcgcc agaacatcgc taccgagctg ctcaagctgc atatcgggga tgtgtgtatc
    11581 ccccggcgta tagatgcttg tcgtgctgag gatgaggtat gctgtaagga gcatgacgag
    11641 ccctgccgcg atcccctcca tggtgtagag ctgccccgaa tcgttcacca tatggcgacc
    11701 tccaggacag cgggcttgag atccggtcgg gtgacgtttg ttggattgta atcatagtag
    11761 tggatgccgg tgatgttggt atctggaggt ttatcctcga agttaaactc tacatccagg
    11821 atgctggtcg gcccgagttc gaatagagat gttgcagctg gtttgaggac gagagagatg
    11881 tttgacgaga ccccaacacc tggctcaggc gggcaaagat tgttgtcgat ccgcagcgtg
    11941 taatagtccg tatcatcttc gtcgtagggg aaggggatgc tcgaagaacc tcgccggaac
    12001 gtcacgctct tgagcgtcgc ttccgatgct gttccgttca ggaacttctc aaaatccgtg
    12061 atcgttatgt tcaccggctc ggttcgcggg tcgatccggt aggcgggatc gatggacagg
    12121 ttaatcaacc ggttgaagtc gaaccgaacg atgaaagtcc tggtggttga gttatcatcc
    12181 ggcgaaatgg tataccggtc cttgaaactt ccattgatca ccgccacgct cggctctttt
    12241 atctttacca cccggcggat gtagccgtag ccctctggag tctcctggcc gaccgcgtgg
    12301 ttgtatctcc cgtccaggct ccggagcgtt atgttgtagg agtaggtgat atcgccaaag
    12361 atcgccttgc tgcggtagtc ttcaggatcg aagaacgagg tgtcgaagaa cttctcaatc
    12421 tttgttgaga ggaggatgtt tggtgcatcc ttcgagaccg caagaccggt gcgctcgatc
    12481 tcgccgctgt ggttcacatc gtatgcctcc cacggtggat cgaccggcca gcccgggtcc
    12541 tcggcgagta taaccccggt acggtaggct accgcgtcgt aatcgatgcc actactctga
    12601 agccctgcaa ggagccctgg aaccatgctt gccaccgcga tcagggccac cataaagatc
    12661 gtgaacccga caaggaagtc gatagagaac aacccctcgt cgttcctcag cactatacga
    12721 ccacctcccc cgtatccgcc cggaaggcga gagtctccat cccggcgtcc gaccgggttg
    12781 taatggtata gaccccctcc gcgagcacaa tatccactgt acttgtgttg aggcgatcca
    12841 ttgccgcccg gattccatcc ttatgcatcg cgaagatctc ttccggcgag aggagttcgg
    12901 tcatattcaa ctcctcttca ggtatgggcg caggaagcga gacctcctgc catcctgccg
    12961 cgccgagggt cagccagcgc cccccctcag cgtccaggag acccagcgtc caggaagagg
    13021 ccagcccttc gaggtccaca cggttcccga tcacctgatg cagcgatgcg ccggtgacgt
    13081 tcagaccgcc ttcggtcccg agcagatcga gttcagcgag cgcctctgcc agggtgagtc
    13141 cctccggctc cggcaggtcg acggccgtga taccgccgcc ctcagacggt gtcgtggaag
    13201 cgtcgagaca gccagacgcg gtgcagaaga tacagactgc ggcaaaaagt gccagaactc
    13261 tcccgacaaa tctcatgtaa aacatccttt ttacacgagg ggatattaat aataacccat
    13321 atcgcatcct ccagaatcgc cgcgaaaacc ggatacccgg tggatgcgga gtggatgccg
    13381 cacgaaattg tatcgggatc tctaatcggg atctctatag tcccccgctt cgggctttat
    13441 acctccggtg caccggccag gggtcatggc acctcaccgt aatatataac agagatatag
    13501 tagcaagaga tcatacagga taggtatgtg caacaactca gacccgggca cgcccggaga
    13561 agcacgcggc cggtttgccg aagcggtcgc gtttcacggc cacgtctgcc ccggcctcgc
    13621 tataggttac cgggcggcgg agatcgccct ctcccggcta tcttccggcc ggtcgagaga
    13681 tgaggaactg gtcacgatca ccgagaacga cgcttgcggc gtcgatgcca tccaggtact
    13741 gacgggctgc accgccggga aagggaacct gctcttcagg gaccatggca aacaggcgtt
    13801 cacgttcatc aaccgtaaaa caggggcagc gatccgggtc gcggcgaatc catcgtttga
    13861 tattgagtcg ctcgaccctg agtttgcgcc gctcagaaag cgcgtgatgc agggcgaggc
    13921 aaccggtgcg gaacgcgcag acttccagga gcacgtgaac cgggtcgtcg atgccatact
    13981 ggaggcacct gacgacgacc tcttcataat ccgcaaggtc gatcagacga tacccgaacc
    14041 cgcccggatc ttccgctcgg tgccgtgtgc gaaatgcggt gagatgaccg ccgagtcccg
    14101 catcagggtc gaggatggaa aatttctctg ctatgcctgc tcggagaatt actccaggtg
    14161 cctgtgaggg gcgcctccat cctcaccgca cgatctttgt tatcgggatc tcaaggatat
    14221 cctcggcacc tgcggctttt agagcaggga tcagcccgtt gacaagccct ttctgcacca
    14281 ctgtctggat gctgaagtag tcgatcccgt gaagcctgct gagggtgggt ctcttcatcg
    14341 ccgggagcac ctcgatgaca cgctcaagcg ccgccgccgg cacgttcatc gtcaggagaa
    14401 cctggcggcg cgcctcgatc acccccatga gaagtgttgc gatctcgtcg atctcccggt
    14461 gcttatgggg gtcgcggagc gagtcgcggt ttgccaggag cgcggtgtgt gactccatga
    14521 tctctcccac gatcttgagc ccgttcttcc ggagggtgct ccccgtctcg gtgaggtcga
    14581 cgaccacgtc catcaggtcc gggaccttcg cctctgaagc cccgtaggag gggaagagcc
    14641 tgaccgggat cccgaggtcg tcaaagaacc gtttcgtgat ccggggatac tcagttgtaa
    14701 cccggctgcc ggggcggata gcggtgacgt cctcgatcgg ttcgtcacgg tgaaccgcga
    14761 ccacaatctt cacgttcccc tctcctgtct tgctgtagga gaggtttgca acctcaacaa
    14821 cgtcggcacc gctctcctcc acccagtcaa ctccggatat cccgagatcg aagtagccca
    14881 tctggacgta gagcgggatc tcctgcggcc ggaggatctt caccttgccg atcctcgggt
    14941 catcgatcct gggattgtaa tcgcggtcgg tccgtctgac ctcgagatcc gcttccttga
    15001 agacctgtag cgtctgcgct tcaaggctcc cttttgggag cgcaatcgtg atcataaaag
    15061 atcatcctgc aggaaaagag ttaaagttgg tgccactcga cttctcagtc tatggtgtga
    15121 acaacaagcc cggaaaggag tttcggctcg aaccatgtgg atttaggggg catgacgccg
    15181 cctgcatcgg ctattgcgag caccgtctcg atcggcaccg gctgcatcgc cacggcgagg
    15241 gcgtatctgc cgtcatccac aagtcgttcg agttcacgga gtggtttcgc cccacccatg
    15301 tagtggagtc tcgtgtcagc ccgtggatcg gagatcccga gcatcccctc caggatatct
    15361 ttctggagga cagagacgtc gagtgacccg atcaggttgg tggggtcagc gatcggccgg
    15421 gagatctcgt accatgcccc atccaggtat atgtgcatca catgccctcc tccagcatca
    15481 ggctcaagcg gcgggatctg gtagccttcg gtatcgaggc tcccgcaggg gcggacggtc
    15541 cagccagatt cacggagcgc ctcgaggaac tcgcggggag tggcgtatct gccaaggtcg
    15601 gtgacgagtc tgctgtaacc gtggatacgt acgcggtcgt gaggaaagag aaccaccata
    15661 aacctcccgg cctcctctgt aaacctcccc tcgcgcctcc gcagagcggc cacgttcacc
    15721 gcggatttgg cacggtggtg cccgtcggcg atataagtct ccggcaccgc cgaaaagagg
    15781 tcttcgaggt gagagagggc ggcatcgtct gtgatccggt aaatctggtg cagcacaccc
    15841 gatgcagtcg tcgtgcttcc gtcgggccgg acaccctcga tgagggagtg aaggtgtggg
    15901 tagataccct gccggtctcg gtagagcagg aacacaaggc cggtgttagc gccgactgca
    15961 tctatatgcc gcgtccggtc ttcctccttg tcataacggg tgagttcgtg ccgccggatc
    16021 ttccctgtct cgtaatcttc tgtcgggacg cagcagacca ggccgacaaa ctcttccccg
    16081 tcctgtactg cccggtagac atacatcccc ggctccgggt cgcgctccat cagcccatcc
    16141 gcgagcatgc tctcgaagac ctctcgcgcc cgttggtaga cccggtcatc gtttggcggg
    16201 atttcgggga gttcggcatc aggccggctt acccgcagga aactcagcgg gttcttctcg
    16261 atgcattccc gcgcttcgtc tgctgtcacc acatcgtatg gtacggatgg gatctcttca
    16321 gagtaccgcc gcgctggacg gatcgccgcg aaacgataaa tttgaaccat ggataccgcc
    16381 actcgtctgt gcctactctt ttgttccctg aagactaata gttgataggt atccaga
//
LOCUS       DAOSZX010000027        16276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_2971_length_16276_cov_19.742124, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000027 DAOSZX010000000
VERSION     DAOSZX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16276
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_16276_cov_19.742124"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..603
                     /locus_tag="PK196_03790"
     CDS             208..603
                     /locus_tag="PK196_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40578.1"
                     /translation="MPTTDPYVTIYQKKTKFEQTIEAYSFNLTRPPLIIEFDVEPKMI
                     TREKHTTSSYGDKDEIVVTQRYPSEDAWFRVIVRDSATGKILAEDGFGKGFSADTHKR
                     IFVGRYGDYLLEFSGNEVTVDIQVRVGGV"
     gene            720..791
                     /locus_tag="PK196_03795"
     tRNA            720..791
                     /locus_tag="PK196_03795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:753..755,aa:Arg,seq:gcg)
     gene            complement(1133..3076)
                     /gene="uvrB"
                     /locus_tag="PK196_03800"
     CDS             complement(1133..3076)
                     /gene="uvrB"
                     /locus_tag="PK196_03800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HON40579.1"
                     /translation="MTGFQLTADFAPTGSQPEAIRKLTAGIESGERFQTLLGVTGSGK
                     TFTIANVIQNVQKPTLVLAHNKTLAAQLYNEFRSFFPGNRVEYFVSYYDYYQPESYIP
                     KRDLYIEKDAQINPRIEQMRLSATASVLSRPDTIVVASVSCIYGLGNPENFRGLGFEL
                     KVGDRMRRDDILRRLVDIQFERNDIELVPGRFRARGDTIDLIPGYFSNIIRIELFGDE
                     IDRISEIDKTSGERVETMDYFFVYPARHFVVPEETKERAIASIEEELDEWLPNLGMIE
                     AHRLRQRTLYDIEMIRETGTCKGIENYSRHFDGRKPGEQPYCLLDYFPHDFLMVIDES
                     HQTLPQVRGMYHGDYSRKKALVEYGFRLPSAFDNRPLKFNEFTDYMRTVIFVSATPGD
                     YELKRSTVVEQIIRPTGLVDPAVEVRPIEGQIPDVMAEIRATIERGDRVLLTTLTKRL
                     AEELSDYLAGQGIKTRYLHSEIDTLERTEIIRQLRLGKYDVLVGINLLREGLDIPEVG
                     LVGILDADKEGFLRDARSLIQTIGRAARNVNSKVVLYADTMTDSIRRALAETERRRAM
                     QIDYNVQHGITPQTIRKPIREKEVDIKDIRHVPKAEIPNLVIELEAEMREAAERLEFE
                     RAIALRDTIRRLQGEGAAKKARG"
     gene            complement(3073..4623)
                     /gene="uvrC"
                     /locus_tag="PK196_03805"
     CDS             complement(3073..4623)
                     /gene="uvrC"
                     /locus_tag="PK196_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HON40580.1"
                     /translation="MIDPATLPAEPGCYLFSDEGGNVIYVGKARNLRKRVSSYFSRHD
                     LDPKTARLIDAIAGIDFIVTDTEVEAFILENTLIKKHQPKYNIDLKDSKTYAYIHITD
                     EPFPRIHVARGPDGNGRYYGPFVSARERDDLLRLLKSLFGLRSCRRLPRRACLRAHIG
                     SCSAPCIGRIGEEDYVERVRRAEAVLKGDIGGLIRTLRDEMAAHAERLDYERAMDLRD
                     QIAALERLRERQHVDRQKTYNEDIINYIRSDGTVYLMLFNVDRGTLAGKHQYTFDETE
                     GFLEEFLARYYADHEPPEEVILPEPVDDAVATYLSHLRGGKVRLVVPQRGEKKNLLDL
                     VRKNVEITFFGDRIKLEELRRRLHLPSLPVAIECFDISHLSGTAMVGSMVRFYCGKPD
                     RRNYRRFRIRSVEGIDDFAAIAEVVRRRYTRLKKEGGEFPDLILIDGGKGQLAAATAV
                     LRELGVSIPIAAIAKREEEVFIPGFSHPLPLDRHEKASLFIQEIRDEAHRFAITYHRN
                     LRRKTVAP"
     gene            complement(4823..7627)
                     /gene="uvrA"
                     /locus_tag="PK196_03810"
     CDS             complement(4823..7627)
                     /gene="uvrA"
                     /locus_tag="PK196_03810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HON40581.1"
                     /translation="MKKIIIRGAREHNLNNITVELPRDRLIVLTGVSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMQKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTVT
                     EVYDYLRLLFARVGKPFCPEHQIPIEAQSPEKIADRIASTMTGTVTILAPIVRQKKGT
                     YQQVFRDLNREGYARVRVNGEIRRTDEEITLERYRKHDIDVVIDRLSVDERSRLAEAV
                     ENALEKAGGLVIAVDSEGREETCSSLMACPVCGMAFEELQPRMFSFNSPFGACEECNG
                     LGIRMEFDPDLIIPDRNKSIAEGAVALYRNYVDGYRVHHLAAVADRYGFSLLTPIKDL
                     TGQQYQALMYGSNERLRFSVEARNGDSSWSHTGRWEGLVPQAERLHSQTRSDYRRREL
                     EKFMRVLPCPRCGGRRLKEKVLAVKVAGKSIVDVTDLSITEALSFFRTLELTETEREI
                     AKQVLKEIIARLEFLEQVGVGYLTLSRSAGSLSGGEAQRIRLATQIGSNLTGVLYVLD
                     EPSIGLHQRDNRKLLETLRHLRDLGNTLIVVEHDEETIRSADWVVDMGPGAGLHGGEI
                     VAEGPPEAIAATPGSLTGRYLAGDLKIDVPARRRRSDRFIRLSGCRANNLKEIDVRIP
                     IGVLTVVTGVSGSGKSTLIYDTLYRALMQRLHNSREPAGEFDSLTFDEEIDKVIVIDQ
                     SPIGRTPRSNPATYTKVFDEIRKVFASTKEAKVRGYNAGRFSFNVRGGRCEACQGEGL
                     IKIEMNFLPDVYVECEECRGTRYNRETLEVKYRGKSIAEVLDMSVEEALALFENVPSI
                     RNKLETLSRVGLGYINLGQSSTTLSGGEAQRIKLTRELSKRATGRTLYLLDEPTTGLH
                     FHDVKNLIAVLDDLVARGNTMVVIEHNLDVIKSADYIIDLGPEGGDAGGEVIATGTPE
                     EVAEVEGSHTGAFLKKILKQ"
     gene            complement(7798..8253)
                     /locus_tag="PK196_03815"
     CDS             complement(7798..8253)
                     /locus_tag="PK196_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HON40582.1"
                     /translation="MKPLDFEKIITSAIDAEVESYTFYKSVAERVKDKSLKTIFLDLA
                     EEEKGHREYLQRVLTRGPKEMQFKAAPDYKVTDALDEPELSVDMKPLDGLLLAIKKEL
                     QAAQMYTRLARASADAEQKMVFEQLANMEKGHKARLEDIYTNMAYPEAW"
     gene            8502..9173
                     /locus_tag="PK196_03820"
     CDS             8502..9173
                     /locus_tag="PK196_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HON40583.1"
                     /translation="MEGDRVAARAETGPRVVEAVQDRHGNQIEIFDPPFYRREFEDAY
                     RFIIDEYAVAPREIALFLPCAVRKPYSQSPSHRLFRRIIDDVLNPADYHIVIFGTCGT
                     VPAELECIYPFCNYHYMLGRTTNERIRRDFHRIEVYRLKGYLEKTRDTYRHRLAYCIG
                     PFRKAMVEASKESGIAVDLLPSDPMIERLYDIDCPFPEGSLSMQGYIDEFREGLVRIA
                     EMVHR"
     gene            complement(9575..10867)
                     /gene="rbcL"
                     /locus_tag="PK196_03825"
     CDS             complement(9575..10867)
                     /gene="rbcL"
                     /locus_tag="PK196_03825"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HON40584.1"
                     /translation="MAIDWYNEFVDLDHTPGTDELVALYYFEPAEGVSKEEAVGRIAS
                     ESSTGTWTTLFTMPPRMRDLQAKAFEIEGNYVKIAYPLALWEEGNATQLLSGIAGNIF
                     GMKALNRLRLIDATLPAGYIQHFKGPHFSIEGIRDMMRVHGRPLTGAVPKPKVGFTAK
                     EHAEIGYETWMGGFDFVKDDENLTSQSFNRFEDRVRAMTDMRDRAEQETGEVKSAFIN
                     ITADTETMKARAEMLADYGWNYAMIDVVVAGTAAVATLRDHCSDLGLAIHAHRAMHAA
                     FDRDERHGITMQFLAKLMRLIGVTQIHTGTAVGKLVGTRAEATLLADMLRERRMNALP
                     HMALEQDWGDIKSAFPVSSGGLHPGLVPDVLDIYGTDLVLLVSGGIHGHPEGTRAGAM
                     AVMQAIEAWQEGESLDEKANHAPELKKALEKWGYYKPK"
     gene            11556..11640
                     /locus_tag="PK196_03830"
     tRNA            11556..11640
                     /locus_tag="PK196_03830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11592..11594,aa:Leu,seq:gag)
     gene            complement(11685..11888)
                     /locus_tag="PK196_03835"
     CDS             complement(11685..11888)
                     /locus_tag="PK196_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40585.1"
                     /translation="MRLTKVEGDHHTEMEIQMVVDCILVDCRLDAAFRDRVVDALIGW
                     AEERPAEWEGLLKRCRQRRSVPV"
     gene            11990..12670
                     /locus_tag="PK196_03840"
     CDS             11990..12670
                     /locus_tag="PK196_03840"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HON40586.1"
                     /translation="MRLGVLFSGGKDSAFACWKAMQKEEVVCLITIVSRNPESYMFHT
                     PNIRLAALQAESAGLPLLEMETKGEKETELLDLRRALLSARERFGIEGVVTGAILSVY
                     QATRLQRVCYELGLWCFNPLWLADQEGYMEELLDAGFRVVITGVFASPFDESWLGREI
                     DHRTLDELRAVARKDRITLTGEGGEFETFVIDAPFFSRRIVIEEGSRVYRNHNGVFRI
                     ERARLVGR"
     gene            12667..13233
                     /locus_tag="PK196_03845"
     CDS             12667..13233
                     /locus_tag="PK196_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40587.1"
                     /translation="MILVIDLCYREDSLSREEFVAPIERLLGRSGREFAARHYKALSA
                     TDIAAADGAILCGTALMDTGFAERPDLFCWLLEFERPVLGISSGMRALAAAFGAGAEP
                     GCGIGMTEVEVLAPDPVFAGKERFSAYEVRGCAVGVPDGFMALAASEGRVQAIRHRSF
                     PLYGLLFHPEVRNEWLIERFLGLVDQRP"
     gene            complement(13221..14447)
                     /gene="mmp10"
                     /locus_tag="PK196_03850"
     CDS             complement(13221..14447)
                     /gene="mmp10"
                     /locus_tag="PK196_03850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867903.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HON40588.1"
                     /translation="MSHLTVDLGGRPGLDCRGFCSYCYFKHVRETTAFGCRHCLPFKK
                     GCDYCTRGVREGYEGFKDLRTVAEETLASLQMMSGDLDRVTISGGGDPSCYPEFYDLI
                     ELLGSMEVPLHIGYTSGKGFDDPGVADHLIDNGLSEVSYTIFAADPGLRRDWMHDPTP
                     EASLAVLDRFCEEIDVYAAAVIIPGVNDGEVLEQTCAWLDERGAKGLILMRFANRTEQ
                     GLILGNAPIIEGQRIHTVDEFQNLVTDLNERFSMKISGTPLGDPSIGSPFAILHEPDL
                     LRKLPRVQKRASVITGSIAAPYIQRILSIRGSTSRVVAVRKEIACLITADDLAGVDLS
                     KLEDVVIIPGRAFVHDAEAKEILSADGVDRDVLRGPDMLTADAETSMGMTRNEVLDKE
                     MEGIAALINTINRYGR"
     assembly_gap    14857..14956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15070..15345
                     /locus_tag="PK196_03855"
     CDS             15070..15345
                     /locus_tag="PK196_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HON40589.1"
                     /translation="MMRTSHRLLLRLYHDPGYDFSRVEIDYVDRGAPGDRSTVRGERV
                     VALEAPYMEVDAGTRTACIPYHRIRRILYDGEVVWEHGHREGDAGDG"
     gene            15396..15468
                     /locus_tag="PK196_03860"
     tRNA            15396..15468
                     /locus_tag="PK196_03860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15429..15431,aa:Thr,seq:tgt)
BASE COUNT         3005 a         4745 c         4849 g         3577 t
ORIGIN      
        1 taccttttat agcagctgcg gttctactgg tcatcatcct cgcctctgcc gggtggaagt
       61 acaggacaat cgggtggaat tccaccaaca gaagaacaga gcagcgggca gggttacaac
      121 ccgggttacc tgaccccggc caccccctat cccacagcgg caccggacgt tcttgctgcg
      181 ccaactctca acaggttctc tgatactgtg ccgacgacag acccttatgt gaccatctac
      241 cagaagaaga caaagtttga gcagacaatt gaggcctact cattcaacct gaccagacca
      301 ccgctcatca tcgagttcga cgtggaacca aagatgatca cccgggagaa acatactaca
      361 agcagttatg gtgataaaga tgagatcgtc gtcacccaga gatacccgag cgaggatgcc
      421 tggttcaggg tgatcgtgcg ggacagcgcg accggtaaga ttctggccga ggatgggttc
      481 gggaagggct tcagcgcgga cactcataaa aggatcttcg ttggcaggta cggggactac
      541 ctgctcgagt tctctggaaa cgaggttact gtggatatcc aggtgcgcgt tggcggcgtg
      601 tagtcagttg gatcaagact gcgttccagg aactcgcatg ttctttcccg ctgaccgggt
      661 gagcaatcct ttttaaccag gacaacccta gttgttaggg taaacggatc cgttcttgtg
      721 tcccggtagg gtagtggata tcctagaagc ctgcggagct tttgacccgg gttcaaatcc
      781 cggccggggc gtattcgatt ttgccgatat ctcagtcgtg cagcaggcct ggattccggg
      841 gaggagtggg ggtggggttt gcttttcccc tcaccgaaaa gttccctcgc tcgcccggga
      901 acgaattcca gggtggtatc accacacagc ccctcgcagc cgacccgctt tcatgcgggg
      961 ttcgccacga agaccctgtt ctatacccct ctctccccgt gagccattgg gcatcatcac
     1021 ccgctgttat cccttaaaga gagaagacga aacgccgccc cggcatcttt tgccgcgcac
     1081 tcgaacgcgg ggagggacgt gtccggtggt gtgctgtgaa cctgatttgc ggtcaccccc
     1141 gggctttctt tgccgccccc tccccctgca gtctccggat cgtgtcgcgg agggcaatcg
     1201 cacgctcgaa ctccaggcgt tctgccgctt cccgcatctc ggcctcgagc tcgatgacca
     1261 ggttcgggat ctcggccttc gggacgtgcc ttatatcctt gatatcaacc tccttttcgc
     1321 ggatcggttt cctgattgtc tgtggtgtga tcccgtgctg aacgttgtag tcaatctgca
     1381 tcgcccgccg ccgctcagtc tccgccaggg ctctcctgat agagtcggtc attgtgtcgg
     1441 cgtagagcac tacctttgag ttaacgttcc tcgcggcccg cccgatcgtc tggatcaggc
     1501 tccgggcgtc gcgcaggaac ccctctttgt cggcgtccag gatcccgacc agtccaacct
     1561 cggggatatc gagcccctca cgcagaaggt ttatcccgac cagcacatcg tacttcccaa
     1621 ggcgcagctg ccggatgatc tccgtccgct cgagggtatc gatctcggag tggaggtaac
     1681 gtgtctttat accctggcca gccaggtagt ccgagagttc ttctgcaagc cttttcgtga
     1741 gcgtcgtcag gagcacccgg tcgccgcgct cgatggtggc cctgatctcc gccataacgt
     1801 ccgggatctg cccctcgatc ggcctgacct ccacagccgg atcaacaagc cccgtcggcc
     1861 ggatgatctg ctcaacgacg gttgagcgtt tcagttcgta gtcacccggc gttgccgaga
     1921 cgaagataac cgtccgcatg tagtctgtaa actcgttgaa cttcaacggc ctgttgtcaa
     1981 acgccgaggg gaggcgaaac ccgtattcca caagcgcctt cttcctcgag tagtcgccat
     2041 ggtacatccc gcgcacctgc gggagggtct ggtgactctc gtcgatcacc atcaggaaat
     2101 cgtgcgggaa gtagtccagc agacagtagg gctgttcccc gggtttcctc ccgtcgaagt
     2161 ggcgtgagta gttctcgatc cccttacacg tcccggtctc ccggatcatc tcgatgtcgt
     2221 agagcgtccg ctggcgcaac cggtgcgcct cgatcatccc caggttcggg agccattcat
     2281 cgagttcctc ctcgatcgat gcgatcgccc gctcttttgt ctcctccggg acgacaaagt
     2341 ggcgggcggg gtagacgaag aagtagtcca tcgtctcgac ccgctctccc gaggtcttat
     2401 cgatctcgct gatccggtcg atctcgtccc caaagagttc gatccttatg atgttgctga
     2461 agtagccggg gataaggtcg attgtgtcgc cgcgcgcccg gaaccggcca ggaacaagtt
     2521 cgatatcgtt cctctcaaac tggatatcca cgagcctccg gaggatatca tcacgcctca
     2581 tccggtcgcc gaccttcaac tcgaacccca gcccccggaa gttctcgggg tttccaagac
     2641 cgtagatgca tgagaccgag gcgaccacta tggtatcagg acgggagagg acagaggcag
     2701 tggcagaaag ccgcatctgc tcgatcctcg ggttgatctg ggcgtccttc tcgatgtaga
     2761 ggtcgcgttt cgggatgtag gactcaggct ggtagtagtc gtagtatgag acaaaatact
     2821 ccaccctgtt gcccgggaag aatgacctga actcgttgta gagctgtgcc gcaagcgtct
     2881 tgttgtgggc gagaaccagg gtgggtttct ggacgttctg gatcacgttt gctatcgtga
     2941 aggtcttgcc cgatccggtc accccgagca gcgtctggaa ccgctcgccg ctctcgatgc
     3001 cggcagtgag tttccggatg gcttcgggct gggatccggt gggcgcaaaa tccgctgtaa
     3061 gttgaaaccc ggtcatggtg ccaccgtctt cctccgcagg ttcctgtggt aggtgatcgc
     3121 aaaccggtgc gcctcgtcgc ggatctcctg gatgaagagt gatgccttct cgtggcggtc
     3181 aagcggcagg gggtgggaga aacccggtat aaaaacctcc tcttcgcgct tggcgatggc
     3241 cgcgatgggt atgctgaccc cgagttccct gagcacggcg gttgccgctg ccagctgccc
     3301 ttttccgccg tcgatcagga tgaggtccgg gaattccccg ccctctttct tcaaacgagt
     3361 gtagcgccgg cgcacgacct ccgcgatcgc cgcgaagtcg tcgatgccct cgacggacct
     3421 gatccggaac cggcggtagt tgcgccggtc aggttttcca cagtagaacc tgaccatcga
     3481 tccaaccatc gccgtccctg aaaggtggga tatatcaaaa cactcgatcg cgaccggtag
     3541 gcttgggagg tggagacggc gtctgagctc ctcgagtttg atccggtcgc caaagaacgt
     3601 gatctcgacg ttctttctga caagatcgag caggttcttc ttctcgcccc gttgcggcac
     3661 cacaagacgg accttcccgc ccctgaggtg ggagaggtag gtggcgaccg catcgtccac
     3721 aggttcgggg aggatcacct cctccggagg ttcatggtcg gcgtagtagc gggccaggaa
     3781 ctcctcgagg aaaccttcag tctcatcgaa ggtgtactgg tgcttcccgg caagcgtccc
     3841 ccggtcaacg ttgaagagca tcagatagac ggttccatcg cttctgatat agttgatgat
     3901 atcctcgttg taggtcttct gccggtcgac atgctgacgt tcgcgcaacc gctcgagggc
     3961 agcgatctgg tcacggagat ccatcgcccg ttcgtaatcg aggcgttccg cgtgcgccgc
     4021 catctcatcc ctgagcgtcc ggatgagccc gccgatatca cctttgagga cggcctcggc
     4081 cctccggact ctctccacgt aatcctcctc gccgatcctc ccgatacagg gagcgctgca
     4141 cgagccgatg tgcgcccgca ggcacgcccg ccggggaagg cgacggcatg aacggagccc
     4201 gaatagagat tttaagagcc ggagaaggtc gtcacgttct cttgccgaaa caaacggccc
     4261 gtagtagcga ccgtttccgt cgggtccacg ggcgacatgg atccttggga acggttcgtc
     4321 ggtgatgtgg atgtaggcgt aggtcttcga gtccttcagg tcgatgttgt acttcggctg
     4381 gtgtttcttt atcagggtgt tctccaggat gaacgcctcg acctcggtgt ctgtgacgat
     4441 gaagtcgatc ccggcgatgg cgtctatcag gcgtgctgtc ttcgggtcga ggtcgtggcg
     4501 cgagaagtaa cttgagacgc gcttccggag gttcctggcc ttacccacgt agatgacgtt
     4561 ccccccttcg tcggagaaga ggtaacagcc aggttcagcc ggaagggttg cagggtcgat
     4621 catggttcag gtgcggtttt ttcggaggga ccgggacccc tccgctctct cgcgtgagtc
     4681 aacaccgccg ttgacggcgg atctctcata tgtgaataca ggtgttggaa tcgataaagt
     4741 ctttgcagag aggggcctct ggggtggtgt tgcgctaacc cggcactgaa tcacagccct
     4801 ggcctctcgt gggtgtcatc tgtcattgtt tcagtatctt tttcaagaac gccccggtgt
     4861 ggctcccctc aacttccgcg acctcttcag gggttccagt cgcgatgacc tcgccgccgg
     4921 cgtccccgcc ctcgggcccc aggtcgatga tgtagtcggc cgactttatg acatccaggt
     4981 tatgctcgat cacgaccatg gtgttccccc gggccacaag atcgtccagg acggcgatca
     5041 ggttcttcac atcatggaag tgcagccccg tagtcggttc gtcgaggagg tagagcgtcc
     5101 ttcccgtcgc ccgcttcgag agttcacggg tcagcttgat cctctgcgcc tcgccgccag
     5161 agagggtcgt tgaactctgc ccgaggttga tgtagccaag ccctaccctg gagagtgtct
     5221 cgagtttgtt cctgatggag gggacgttct caaagagggc aagcgcctcc tcgacgctca
     5281 tatcgaggac ctcggcgatt gatttacccc tgtacttcac ctcaagcgtc tcgcggttgt
     5341 aacgtgttcc ccggcactcc tcgcactcga cgtagacatc agggaggaag ttcatctcga
     5401 tcttgataag cccctctccc tggcaggcct cgcacctccc gccgcgcaca ttgaacgaga
     5461 accgcccggc gttgtagccc ctgacctttg cctcctttgt cgaggcaaag accttcctga
     5521 tctcgtcaaa gaccttggtg taggttgccg ggttcgagcg cggcgtccgg ccgatggggc
     5581 tctggtctat gactatcacc ttgtcgatct cttcatcaaa cgtgaggcta tcaaactcgc
     5641 cggcgggttc gcgtgagttg tggagacgct gcatcagtgc gcggtagagt gtgtcataga
     5701 tgagcgttga cttccctgaa cccgagacac cggttacaac cgtcaggacg ccgataggga
     5761 tcctgacatc gatctccttg aggttgtttg cccgacagcc ggagagccgg atgaaccgat
     5821 cgctcctcct gcgcctggcg gggacgtcga tcttcagatc gccagcaagg tacctccccg
     5881 tgagcgaacc aggtgtcgcc gcgatggctt cgggcggacc ttccgcgact atctccccgc
     5941 cgtggagccc cgctcctggt cccatgtcca ccacccagtc ggcgctccgg atggtctcct
     6001 cgtcgtgctc aaccacgatc agggtgttgc caaggtcgcg gaggtgccgg agggtctcga
     6061 gcagtttcct gttgtcgcgc tggtgcagtc cgattgaggg ctcatccagg acgtagagga
     6121 cgcctgtaag gtttgatccg atctgtgtcg ccagacgtat cctctgcgcc tcaccaccgg
     6181 agaggctccc tgcgctccgc gagagcgtca ggtagccgac acccacctgc tcaagaaact
     6241 cgagacgggc gatgatctct ttgaggacct gctttgcgat ctcgcgctcg gtctcggtga
     6301 gctcgagcgt cctgaagaac gaaagcgctt cggttatgga gagatcggtg acatcgacga
     6361 tggactttcc ggcgaccttc acggcgagga ccttctcctt gagcctgcgg ccgccgcatc
     6421 ttgggcatgg gaggacgcgc atgaacttct cgagttcccg gcgccggtaa tcggatctcg
     6481 tctggctgtg gagccgttct gcctggggca caagcccctc ccatctcccg gtatgcgacc
     6541 aggaggagtc gccgttcctc gcctccaccg agaaccggag tctctcgttc gagccataca
     6601 taagtgcctg atactgctgc ccggtaagat cctttatcgg cgtcaggagg gagaacccgt
     6661 atcggtcggc caccgcagcg aggtggtgca cccggtagcc gtcaacgtag ttccggtaga
     6721 gcgcgacggc gccctcggcg atgcttttgt tcctgtcagg tattataagg tcggggtcga
     6781 actccatccg gatcccgagg ccgttgcact cctcacaggc accgaacggg ctgttgaagg
     6841 agaacatccg gggctggagt tcttcaaacg ccatcccgca gaccgggcag gccatgagcg
     6901 aggaacaggt ctcctcgcgc ccctcgctgt cgacggcgat gaccagcccc ccggcctttt
     6961 caagggcgtt ctcaacggct tcggcaagcc ttgatcgctc gtcgacggag aggcggtcga
     7021 tgacaacatc gatgtcgtgt ttgcggtaac gttccagggt gatctcctcg tctgtacgcc
     7081 ggatctcccc gttcacccga actcgcgcat aaccctcacg gttgaggtcg cggaagacct
     7141 gctggtatgt ccccttcttc tgccggacta tcggggcaag tatggtgacc gtcccggtca
     7201 tcgtggaggc gatccggtct gcgatcttct caggtgactg cgcctctatg gggatctggt
     7261 gctccgggca gaagggtttt ccaacccggg caaagaggag ccgcaggtag tcgtagacct
     7321 cggtgacggt gccgacggtg ctccgtggat tctttgatgt agtcttctgt tcgatggata
     7381 tggcaggaga gagcccttcg atgctgtcga cgtctggttt ctgcatcaga cccaggaact
     7441 gtcgtgcgta ggcggagagt gactccacgt agcgccgctg cccctcggcg tagatggtgt
     7501 caaaggcaag cgtggatttt cctgacccgg atacacctgt gaggacgata agccggtcgc
     7561 gcgggagttc gacggtgata ttgttgaggt tgtgctcccg cgcccccctg atgatgatct
     7621 ttttcatttg ggtatccagg tatttttgtc ttgcttcctc atagccattt gctaatggaa
     7681 agttttaaat atatagaata aaagggaggc cgatgaccgg gcggcatcgg atgttccggc
     7741 cgcctctgtc acggcagttt gagcgtgagt ggatgttgtt gtgtgaggcg gtgcctatca
     7801 ccacgcctct gggtatgcca tgttcgtgta gatgtcctcg agccgggctt tgtgcccttt
     7861 ctccatgtta gcaagctgct caaagaccat cttctgctct gcgtctgcag aggctctggc
     7921 cagccgggtg tacatctgtg cggcctgcaa ttctttcttg atggccagga gcagaccgtc
     7981 gagcggcttc atgtcgacgg agagttctgg ctcgtcgaga gcgtctgtga ccttgtagtc
     8041 gggggccgcc ttgaactgca tctcctttgg gccgcgggtc aacacccgct gcaggtactc
     8101 gcggtgaccc ttctcctcct cggcaagatc aaggaagatg gtcttgaggc ttttatcctt
     8161 taccctctcg gcaaccgatt tatagaaggt gtacgattcc acttcggcgt caatggcact
     8221 ggtgatgatc ttctcgaaat ccaggggctt catatctata cctccatgat cctctgcgat
     8281 agcagagtct gttcgcgttc attcgaacaa tgctatatag gatattgaac ggttaaatct
     8341 tttgccttta ttactccagg agggggtcgc cctctcactc tggctgcgct gcagatctct
     8401 tgcacgcaac tcccaccggg cggcaggaat acgcccttcc agaatgcttt ttccccttcc
     8461 cggatcaaac ctctacccgg ttcagggggt aatctccagt catggagggc gaccgggtgg
     8521 ctgcacgtgc ggagaccgga ccgcgggtgg tcgaggctgt gcaggaccgg catgggaatc
     8581 agatcgagat attcgacccg ccgttctacc ggagggagtt tgaggacgca taccggttta
     8641 tcatcgatga gtatgcggtg gcaccaagag agattgccct ctttctcccc tgtgcggtgc
     8701 gcaaacccta cagccagagc ccgagccatc ggctttttcg ccggatcatc gatgacgtcc
     8761 tgaatcctgc ggactatcat atcgtcatct ttggcacctg cgggacggtt cctgcggaac
     8821 ttgagtgcat ctacccgttc tgtaactacc attacatgct cggcaggacg acgaacgagc
     8881 ggatccggcg cgactttcac cggatagagg tctatcggtt gaaaggttac cttgagaaga
     8941 cccgggatac ctaccggcac cgacttgctt actgcatcgg gccgttcagg aaggcgatgg
     9001 tcgaagcctc aaaagagagc gggatcgctg tcgacctgct cccctcggat ccgatgattg
     9061 agcgactcta cgatatcgac tgtccattcc cggaggggag cctctcgatg cagggctaca
     9121 tcgatgagtt ccgggagggg ctcgtgagga tcgcggagat ggtgcaccgg tgaggtcttg
     9181 agatggcctc gaaacactcg tgactgcccc caatccatcg tcggcggtct tatgcctctg
     9241 tttttttgca aaaaccagat catccggttc ttccagcgcc tccatgcccc agagcctgtg
     9301 gattggcggg caacgagcga cgagcgccag gagaagccaa atcctggacg cagaggaatc
     9361 tcatctggtc taaacgtgac ctgaacactg aacgtcttct ccggggcact ggaccgtggg
     9421 aaaatcaaaa atagggaaag ggtggaatgg cctccctctt ttcagcgtat ccggtgaact
     9481 caggcttcac cctgaggggg attttctgca gcgtgcaggc cacacccgga aaaaccgcct
     9541 tttgagaagc cctcaaaggc tcttgagccg gcagtcactt cggcttgtag tagccccact
     9601 tctccagggc tttcttgagt tccggtgcat ggtttgcctt ctcgtcgagg ctttccccct
     9661 cttgccaggc ctcgatcgcc tgcatgacgg ccatcgcacc ggccctggtc ccctcggggt
     9721 ggccgtggat cccgccgctc acaaggagga caaggtcggt tccgtatata tcgaggacgt
     9781 ccgggacgag cccgggatgg agtccgccgg aggagacagg gaaggcgctt tttatgtcgc
     9841 cccagtcctg ctccagggcc atgtgaggta aggcgttcat gcgccgctcc cggagcatgt
     9901 ccgcaagcag cgtggcctcg gccctggtgc cgaccagttt acctacggca gtcccggtgt
     9961 ggatctgtgt aacgccgatc aaccgcatca atttcgccag aaactgcatg gtgatcccgt
    10021 ggcgctcgtc ccgatcgaag gccgcgtgca tcgcgcggtg ggcgtggatt gcaagcccga
    10081 ggtcggagca gtggtcacga agggtcgcga ccgcggccgt ccctgccaca accacatcga
    10141 tcatagcgta attccagccg tagtccgcaa gcatctccgc ccgcgctttc atagtctctg
    10201 tatcagccgt tatgttgatg aacgcggact taacctcacc tgtctcctgc tcggcgcgat
    10261 cccgcatatc tgtcatcgcg cggacgcggt cctcaaacct gttgaacgac tgggatgtca
    10321 ggttctcgtc gtccttgacg aaatcaaacc cgcccatcca ggtctcgtaa ccgatctcgg
    10381 catgctcctt tgcggtgaaa ccgaccttcg gttttgggac agcgcctgtg agcggccggc
    10441 cgtgaacccg catcatatca cggatcccct cgatgctgaa gtgcgggcct ttgaagtgct
    10501 ggatgtagcc ggccgggagc gtggcgtcga tcaaccggag gcggtttaac gccttcatgc
    10561 cgaatatgtt cccggcgatg ccgctcagga gctgggtggc gttcccctct tcccagaggg
    10621 cgagggggta tgctatcttc acgtagttcc cctcgatctc gaaggccttc gcctggaggt
    10681 cgcgcatccg tggtggcatg gtaaagagcg ttgtccaggt cccggtcgag ctctctgagg
    10741 caatacggcc aaccgcctcc tccttgctga ccccttcagc gggttcgaag tagtagagtg
    10801 ctacaagttc gtccgtgccg ggggtatggt cgagatcaac gaactcattg taccaatcaa
    10861 tcgccataac atcacctttg aagtgaaggc tttcaggggt gaaaaacctt ccatccgatg
    10921 acggcaaaaa accaggtgag aaggcgtgtt tctcatccgg cactggggcg gacggaaaag
    10981 agggcgttgg ctgggggaaa cgcgtgtgag gatggttgta ttacatgtgt ttaattttgt
    11041 catattaggt aaacatatat aacatgtatt tcatactagc aatgggtcgg gccaccactg
    11101 atggaaacct tccctctgca cgtccaccct accatatctt taaccaattt ttttggccgg
    11161 acaccagcga gggtgtgtcc tgccctggca cgcaccgtga actgatatcc ccgttttgca
    11221 attcgccatt gatgcagtgc caggaagtgc atctccaccg tcacggtaaa agatctctga
    11281 ggaatacagt ctccgcccag gcacaatttt cccctgatct tcgccaatct gctgtccgcc
    11341 ttgggttggt agagtatatc gcgaaaggcg tacacagagg cggtcttgac agctccccgc
    11401 agataccgcc gagaaagagt ggctagttta cagtccagat cgttgatacg atgccacccc
    11461 gccccgcaca aatgtatttt atgcaggaaa tctgattgag tgaggcgaac ggggtgcggc
    11521 aacgcaaccg atgcgtcagc cgtcaccgta tcggtgcgag ggttgccaag ccaggtcaaa
    11581 ggcgccaggt tgagggcctg gtcccgcagg agttcgtgag ttcaaatctc actcctcgca
    11641 ctccggtttt tcaagcaacg cagcgggaga agatcccgcg atctttacac cggcaccgac
    11701 cggcgctgcc ggcagcgttt aaggagtccc tcccactcgg ccgggcgctc ctccgcccac
    11761 ccgatcagcg catcgaccac ccggtctcga aacgcggcgt ccagccggca gtcaaccagt
    11821 atacagtcaa ctaccatctg aatctccatc tcagtgtggt ggtcgccttc aaccttcgtg
    11881 agcctcaccc tcaaagcggg tgaacgtcag aatagtatat aaaaatgatc cttccgctgc
    11941 cgacgggagg gcttatcgtg ctctgcgcag aagattacgg gaacctggta tgagactcgg
    12001 cgttctcttc tccggcggga aagactcggc cttcgcctgc tggaaggcga tgcagaagga
    12061 ggaggtggtc tgcctgatca cgatcgtctc ccggaacccg gagagttaca tgttccacac
    12121 cccaaacata cggcttgccg cgctgcaggc cgagtccgcc ggactgccgc tcctggagat
    12181 ggagacgaag ggcgaaaaag agaccgaact gctggacctt cggcgtgcgc tcctttccgc
    12241 acgggaacgt ttcgggatcg agggcgttgt gaccggggcg atcctctcgg tctaccaggc
    12301 aacgcgtctc cagcgggtct gctatgaact ggggctgtgg tgcttcaacc cgctctggct
    12361 tgcagaccag gagggctaca tggaggaact cctggatgcc gggttccggg tcgtcataac
    12421 gggcgtcttt gcctcgccct tcgatgagtc gtggctcggc agggagatcg accaccgcac
    12481 cctcgatgaa ctccgggcgg tcgcccgcaa agaccggatc accctcacgg gtgagggcgg
    12541 ggagttcgag acgttcgtca tcgacgcccc gttcttctcc cgacggatcg tgatcgagga
    12601 ggggtcgagg gtctaccgga accataacgg cgtcttcagg atagagcggg cgcggctggt
    12661 gggacgatga tcctggtcat cgacctctgt taccgcgagg actcgctctc gcgggaggag
    12721 tttgttgcac cgatcgagcg gctccttggg agatccggca gagagtttgc cgcccgccat
    12781 tacaaagcgc tcagcgcaac cgatatcgcg gccgcggatg gggcaatcct ctgcggcact
    12841 gcgctgatgg acaccgggtt tgcggagcga cccgacctct tttgctggct ccttgagttt
    12901 gagcgtccgg tgctcgggat atcctccggg atgcgggcgc tcgccgcggc gtttggcgcc
    12961 ggggctgaac ccggctgcgg gatcgggatg acagaggtgg aggttctcgc ccccgatcca
    13021 gtctttgccg ggaaagagag gttctccgct tacgaggtgc gtgggtgcgc cgtcggtgtg
    13081 cctgatgggt tcatggcgct cgcggcctca gaaggccgcg ttcaggcgat ccggcaccgc
    13141 tcgttccccc tctacggcct cctattccac ccggaggtga ggaacgagtg gttgatcgag
    13201 cggttcctcg ggctggtgga tcaacggccg tagcggttga ttgtgttgat cagggccgcg
    13261 atcccttcca tctccttatc gagcacctca ttgcgggtca tccccatgct cgtctccgcg
    13321 tcggccgtca gcatatcggg gccgcgcaga acgtcgcggt cgacaccgtc ggcggagagg
    13381 atctcttttg cctcggcgtc gtggacgaac gcccggccgg ggatgatcac gacatcctcg
    13441 agttttgaga ggtccacacc cgccaggtcg tcggcggtga tgaggcacgc gatctccttt
    13501 ctgaccgcaa caaccctgga ggttgaaccg cggatcgaga gtatccgctg gatgtatggg
    13561 gcggcaatgc tcccggtgat aacgctcgca cgcttctgga cgcgggggag ttttctgagg
    13621 aggtcgggct cgtggaggat ggcaaacggc gaaccgatgc tcgggtcgcc aagcggggtt
    13681 ccgctgatct tcatcgagaa acgttcgttc aggtcggtga cgaggttctg gaactcgtcg
    13741 accgtgtgta tgcgctgccc ctcgatgatg ggggcgttgc cgaggatgag cccctgttcg
    13801 gtgcggtttg caaaccgcat aaggatcagc ccttttgcgc cccgctcatc cagccaggcg
    13861 caggtctgct ccagaacctc gccgtcgttc acaccgggga tgatgacggc ggcggcatag
    13921 acatcgatct cctcacagaa ccggtcgagg acggcgagcg aggcttccgg cgtggggtcg
    13981 tgcatccagt ctcgccggag cccggggtcg gctgcaaata tggtgtatga gacctcggag
    14041 aggccgttat cgatgaggtg atcggcgacc ccggggtcat cgaacccctt gccgcttgtg
    14101 tagcctatgt ggagcggcac ctccatgctc ccgagcagtt cgatgaggtc atagaactca
    14161 gggtagcagc tagggtcgcc gccgccgctg attgtgacac ggtcgaggtc tccgctcatc
    14221 atctggaggc ttgcaagcgt ctcctcggcg accgtcctga ggtctttgaa accctcgtag
    14281 ccctcacgca cgccgcgggt gcagtagtca cagcctttct tgaaggggag gcagtgcctg
    14341 cagccgaagg cggttgtctc cctgacgtgt ttgaagtaac agtacgagca gaacccccgg
    14401 cagtcaaggc cgggcctgcc cccgagatcg acggtgaggt gcgacatcct gctgttactt
    14461 ctgtgttact ctgtattgaa cgttgcacag agggtatcaa tccgatacgc atcctggttt
    14521 aaggttgggg atctctatgg acggattttg gggtttttgc gagacggcat tggaggatat
    14581 ggaaatggag gtggggggct ccatagagag aggttttcag tggcacgcct agacagggga
    14641 gggggatgtt caattcccct ccccgtcagc ctctcccccg attgacggta tcccatcgat
    14701 acagtgtcat ggagagcatc aaccagtatg ccggtggatc ccctcgtcaa gatcctctcc
    14761 ctgtcccccc ggtgcacggt atccgggtca atcactaggc actgcccgcc agcctcgtat
    14821 cgaggggggc ctgggtggtg gtttttgtgc atcagannnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnctgc ccgccagcct cgtatcgagg ggggcctggg tggtggtttt
    15001 tgtgcatcag aaaagaggct tgatgacctt gcacatttat gccccgccgg tgctatctgg
    15061 tcatggtgaa tgatgcggac aagtcaccgg ctgcttctac ggctctacca cgaccccggg
    15121 tatgacttct cccgtgttga gatcgattac gtagaccgcg gtgcgcccgg cgaccgctcg
    15181 acggttcggg gcgagcgggt cgttgcgctc gaggcgccct acatggaggt ggacgccgga
    15241 acccgcaccg cctgcatccc ctatcaccgg atccggcgga tcctctacga cggtgaggta
    15301 gtctgggagc acggccaccg cgagggtgac gccggcgatg ggtgagcggc accggcgcca
    15361 ccttttttac cgcagacctc ttaccattag ggtatgcccc agtggcttag ctggcagagc
    15421 ggctgacttg taatcagcag gtcccgagtt caaatctcgg ctggggctct caagaagacc
    15481 tcttttatcc gccgggaata cccagtcccg gtgaagttga tgctcggcac aagaaacctg
    15541 tatcgctcat caacgcccct cacgacgcga tgcctgaaaa cgggtgaccg ctcccgggaa
    15601 gaaggtgctt ttggatcatc ctccttgcct gctcgggacc gcggaagccc cggagaacct
    15661 tattacgatc gtctgcctgg acaacggggc ggtcggggac accgggaacc agtcgacggg
    15721 cggtggatat gaaactcctc gcccgagccg ccgggatccg ggagcggttt atgcgggcgc
    15781 ttgggaggta ggtgttcggc tagtttctgt ggtggagcga aagcaccccc tcttccttgt
    15841 gcatatcaac tctggacgcc tgtgagtctc tgcctggtgc atcccgaagc atgttttctg
    15901 ggatagaaat cagtgtagtt tctgtgagaa atgggctatc tccagcactt ctcttttcaa
    15961 ctgagggatc agtctcttca ttttcgggaa cgacccctgg atgtgtaaag agatgtgaca
    16021 ggatttacat atagaccggc gtccaggtca tagtgtgggc gcctcgatga tgtgtccaca
    16081 aaagtccgcg ggggtccctc catccacggt tgacgcccgg atgatagagc cccgcatgcc
    16141 cggagggcaa tgagatgtac gcattccagg tacttaaaat gtgcgcctct cctgtctcca
    16201 gggacgttac aggagggaaa acacgtcaac taccccaccc taaagggtgg ggcttgctgc
    16261 cggggcccct tgcggg
//
LOCUS       DAOSZX010000028        15510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3186_length_15510_cov_21.816564, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000028 DAOSZX010000000
VERSION     DAOSZX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_15510_cov_21.816564"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..545
                     /locus_tag="PK196_03865"
     CDS             114..545
                     /locus_tag="PK196_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HON40590.1"
                     /translation="MPGRPSTGEDSTILRWMRFEIGRINNGVVAERKRLSTLLSEERP
                     STITKGGECYEFDRGVLLRLKETLPAELQRRLRLPVLFYFDSTVPDSLFLSDEAAVEA
                     LQHLGEISPLRRMHEGRVWIAKPIVYVIMSRYPTAIQIVMR"
     gene            complement(609..1211)
                     /locus_tag="PK196_03870"
     CDS             complement(609..1211)
                     /locus_tag="PK196_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40591.1"
                     /translation="MSEKELKIGDIAHLTGLTEQDVRRLIQTYESLFTYRTIGPVRLF
                     PQRAVKIVRELAELSGKGLSPDEITERLKSGKKPAAPEEEAAEEAGPAAAPLPAEVVI
                     DLGVMRDTLALQERRIARLSEELDRERRERVSETAQLRKAVGDLEERLSEQKRHLALV
                     AEWVDYFDRQMDEITRPLFERLRRGATKKSDPGQPSNRSG"
     gene            1340..2026
                     /locus_tag="PK196_03875"
     CDS             1340..2026
                     /locus_tag="PK196_03875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HON40592.1"
                     /translation="MKRREEESTPRGDLTIIRKKVRAARLQVQPDGTLRVVAPPSFDV
                     KGFLERNADWIERRQDELDRLAAEGCGREDLLLLHGRFYRLLPGARFAIDVPGETVTC
                     PSIWSLRQNLSLLLREEIAKVVEAHTDPLSRKAGRITVKMQRTRWGSCSTLNNLNFNL
                     RVIALPKSLREYIVVHEVAHLREQNHSQRFWRLVADRCPDYLEAEAELRRYWVILERN
                     RVWRRLQDSR"
     gene            complement(2437..3117)
                     /locus_tag="PK196_03880"
     CDS             complement(2437..3117)
                     /locus_tag="PK196_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HON40593.1"
                     /translation="MRQIEIWGDDDADDRTNVLKVLIPFLLCGLFLSVVWFLLPVGGW
                     LFLFAMMVAYVVPPLGRESVIPLCILGGIPWWLAAFTIVFLDFAGGLFMAWNFPLLMR
                     IPRVGPWISRFVEAGRSFLDRRPWLERLYFVGLIAFVALPFDGSGSIVGSIVGRMLGM
                     AKTEVLTCIVIGAVIGSYAIALGIDWVVNLIPPGAGFLVSISIFLLICATLLLTYRNH
                     YQRPEAEG"
     gene            complement(3169..3606)
                     /locus_tag="PK196_03885"
     CDS             complement(3169..3606)
                     /locus_tag="PK196_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HON40594.1"
                     /translation="MRRVVATGTFDILHPGHLYYLEESRKLGGELYVIVARDANVKHK
                     PKPVIPEDQRLQMVAALKPVDHAILGDLHDMFRPIAEIRPDIITLGFNQHFDEERLRQ
                     NLGERGLDAEVVRIAGYFGSLASSSKIIERILNTRYRSGPGGS"
     gene            complement(3603..3974)
                     /locus_tag="PK196_03890"
     CDS             complement(3603..3974)
                     /locus_tag="PK196_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HON40595.1"
                     /translation="MAIRGISRGLLEMLFELGRQHHPNEFVAVLRERNGVISDLDLVP
                     GTIVGEKSASFFIDMLPLDTHQAGSAHSHPNGLLSPSPADLRFFPSVGRYHIIIGPPY
                     TQADWRCYRADGSVTDLEVIG"
     gene            complement(4043..5467)
                     /locus_tag="PK196_03895"
     CDS             complement(4043..5467)
                     /locus_tag="PK196_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HON40596.1"
                     /translation="MRILLPTGTATAGIVKAAAEQFAGRHEMDVVATGEIAAFLSPRD
                     LRRLLASGDYDMVIVSGMCTASFADVERATGVPVYRGPRHAADLALILPMLDTITLSR
                     TLPADEFLAERRREEAYRQVAHREAEAEPDFVIRGLKIGGSSRMKVLAEIMDAHMRDD
                     LLADVRRFFEAGADIVDLGFGFDATAEDVRRCFSALSGVKAPLAVDTQDPDLIEAALN
                     RADLVLSLHEGNIPLIGRAVADAGAAAVVVPRERTLAENVAAAREVGVRHLIADPLLL
                     PVGSGLTGSLARFSDAPRPLFFGAGNVVELLDADSPGSNALLAGMAHEVGAAVIFTSE
                     HSDKTQGSVAEMRRATEMMALMTGRPYPKDLGLDLLILKEKRRRREPPLDYESVMEAS
                     PAPDEVVYDPLGCIRIGVEGDSIVAVHRGRAVRGKCWEDVFCALLSNGSLSRLDHAAY
                     LGKELYKAELAIRLGRSFEQDGPF"
     gene            5510..6175
                     /locus_tag="PK196_03900"
     CDS             5510..6175
                     /locus_tag="PK196_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HON40597.1"
                     /translation="MLRCDLHIHTRFSRDGESSVEEILRRAEQIGLDAIAITDHDTVE
                     GALYAMECDTPVIVIPGTEVSTQQGHLLALGVTEPIPAGRDFFETVALARSRGALLIL
                     PHPYHRWRHGVGRRLAAGIEAVDAVEVFNSRYITGSANRKAALIARKFGKPGIAGSDA
                     HNARYVGFGVTYVVAEPDAASIISAIREGRTMASGRMTPLHTYTRQSLKGAIRKIRRT
                     VYR"
     gene            6172..6975
                     /gene="truA"
                     /locus_tag="PK196_03905"
     CDS             6172..6975
                     /gene="truA"
                     /locus_tag="PK196_03905"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HON40598.1"
                     /translation="MINLALRFSYFGDRFSGSQMQPGLRTVEGEFIEACRRIQVFDDW
                     REARFATAGRTDRGVHARRQVCSFLTDLPERAIQVLNRVLPGDIWCTGWAEPPEGFHP
                     RYSARSRTYRYYFFPAPEKIAAMNDAAQVFIGRHDFSSFARSSGRDPERRILAARVFL
                     EGRFAVFEVTAESFLWNMVRRMATVLEAVGSGESDAAEIERLLAEPTEQRVAAAPPWG
                     LILWDIDYGMTFNPIQIDVKSGRYLASRLRYHAVMACAADRLAPDDRAV"
     gene            7053..7967
                     /locus_tag="PK196_03910"
     CDS             7053..7967
                     /locus_tag="PK196_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON40599.1"
                     /translation="MTDISRRSVTNLSAIPKEEYIYKCTSACAGCSSSLCLRYVLKAA
                     GPDSVLVVPACCTSVIQGIYPATAMNVPVYNVAFAAAAACASGMSEAFAATGKETNVI
                     VYAGDGGTVDIGIQSLSGAFERGTNFLYICYDNEAYGNTGMQRSGSTPIGARTTTTPG
                     GKPTPKKDLDRIVEAHNPPYMATACSAYPLDLYKKVRKALSIKGPKFIHILAPCPPGW
                     RYPTEKTVEMGKLAVKTGVWVLYEREFGKLTINGPSKAAMRKPEPLDEYIRGQGRFKN
                     LSPETLNLMRQQVEQNIQRLAREEEGIC"
     gene            7961..9073
                     /locus_tag="PK196_03915"
     CDS             7961..9073
                     /locus_tag="PK196_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HON40600.1"
                     /translation="MLTIATGNRAVAEAVKAAKPGVIAAYPITPQTEIVEQIADYVTT
                     GDLDSRYIPVESEHSAMAACIGASVGGVRTFTATSSHGLLYMHEMIHWAAGGRLPIVM
                     ANVNRALGPGWNTWAEHTDAFSQRDTGWLQVYVGTVQEAYDATLMAFRIAEDERVLLP
                     VMVNLDGFSLSHITQSLETEEIGDFLPPYHLPHAIDTENPRGYGPMTGPDNYYRFRWD
                     IERSMRDARDVIAEVESEFADRFGRSYGPTEEYRCEDADVVVIALGTFGKEAEVAVDR
                     LRDEGIRAGSMRLRWFRPFPDLDLSGREVVVIDRDYSFGFGGVVAHSIRAKTGIEPYS
                     VIAGLGGQEVTYNDIADFVRGRRPGEETWFGVNDHV"
     gene            9066..9593
                     /locus_tag="PK196_03920"
     CDS             9066..9593
                     /locus_tag="PK196_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON40601.1"
                     /translation="MYEVRIHSRGGQGGVTAAKLLALAAFRDGKHATACPFYGAERRG
                     APVVSFVRIDDEPIKVYSQIHEPDLVIVLDASIMDVVDVLEGLKEGGTVLLNSAHPVD
                     GCRGTCYHVDLTGIALAQNLVVAGSPILNTPVLGALAKMGVITLPSALQAIREMFDDE
                     RNVKAAVAAYEELKV"
     gene            9590..9835
                     /locus_tag="PK196_03925"
     CDS             9590..9835
                     /locus_tag="PK196_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40602.1"
                     /translation="MRERLALSRPGEAASGKTGSWRTFRPVVDKEACNACGLCAQYCP
                     DGVIDEELNIDLEFCKGCGICANECPKQAIAMVREER"
     gene            complement(9813..>10158)
                     /locus_tag="PK196_03930"
     CDS             complement(9813..>10158)
                     /locus_tag="PK196_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40603.1"
                     /translation="CGEVADQIIKAVSAQAQVVCSACGATRIFIPRFENTGISGISTP
                     IGRCDPWSIETDGYCKHCAVTGPHDLAIGSGHFTTRCRNCGYTHFYRLNLEYIAQCPI
                     DDEGEVSAPPSP"
     assembly_gap    10159..10258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10259..12106
                     /locus_tag="PK196_03935"
     CDS             <10259..12106
                     /locus_tag="PK196_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HON40604.1"
                     /translation="DQVYMAAAQALTDTLGWPLTIIMTPDKKPFFAASYIPKESRYGM
                     TGLIDLIPRIRRLWETRRQDLENTGNLLLEALQNAARTPQNGPEITKGVLDEAYSIFL
                     RIFDRDNGGFGNPPWFPAPHNLIFLLRYWSRTGKEPAYMMAAKTLSAMRQGGIFDQIG
                     YGFHRYSTDAEWFVPHFEKMLYDQALLALAYTEAFLASGKEEFERTARETITYVLRDM
                     TDPGGGFYSAEDADSEGEEGRFYLWTKNEILEILGQEDGEMFSRIFGVTGPGNYLEQP
                     GGRRTGRNILRLRRPLSALAEEFETTEEELAGFVEEARKKLFASREQRVHPAKDDKIL
                     TDWNALMIAALARAARVFDDPEYLAAAERATAFLLNNLRRPDGRLLHRYRQGEAGLAA
                     TLDDCAFMLLALIEDYEASFSPKYLQTALELANDLIEHYQDPDNGGFFFVADDADTPV
                     RQKPVYDGATPSGNSAAMYALYLLGRMTANLELEETAERIHRAFAGLVTRSPAASPSF
                     LTGVELMKGEKTEVVIAGVPGAEDTAAMVRKIYSHYIPGSVVILRPAGEERPEIAEIA
                     GFTRDMVMIDGRATAYVCTNHACRIPTTDPDEMLRAAGTGGIRRHQPGV"
     gene            complement(12141..12911)
                     /locus_tag="PK196_03940"
     CDS             complement(12141..12911)
                     /locus_tag="PK196_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40605.1"
                     /translation="MILNVDGVAFSYRSSPVIRDITFDLRPHQVLAILGPNGVGKTTL
                     LKCMNAILRPKVGSILVEGSEILKADRLSIARKVGYVPQRCEAGRMTAFDAVLLGRRP
                     HIGWDVTERDLRIVEAALRMLDLENLALRYIDEMSGGELQKVSIARALVQEPRLLLLD
                     EPTSSLDLKNQLEILGIIRRVTREHNVAAVMTMHDLNMALRYADRYILLKDGIIHAAG
                     GPEVVTPETIEAVYGVPVTVERYNGYRVVVPLEPGDGC"
     gene            complement(12908..14020)
                     /locus_tag="PK196_03945"
     CDS             complement(12908..14020)
                     /locus_tag="PK196_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON40606.1"
                     /translation="MHLADGVIPADYQGYVRRKRLWILGGVVALALLLIVSISVGAVA
                     IPPYEVFLSLLNGSIDRINLLFNPGGTLDLSSTDRIVWNIRLPQALAAIVAGVGLSVA
                     GVAMQSILRNPLGSPYTLGISNAGAFGAAVSVILLGTGQMHSTAADAVRLSNPYLTTV
                     VAFIFCLLATAVILLISRIRGTSPEVMVLAGVALSSLFTAGTMFLQYFADDTQLSAVV
                     FWTFGDVGRATWQELGIMAFVTLAGSLYFIANRWNYNAIDAGDETAKGLGVNVEAVRD
                     IGMVVAALLSAVIVSFLGVIGFVGLVCPHMVRRLIGDDQRYLIPGSCVMGGLLLLASD
                     TVARVIIAPYVLPVAVLTAFLGAPVFIYLLLRGYRR"
     gene            complement(14025..15209)
                     /locus_tag="PK196_03950"
     CDS             complement(14025..15209)
                     /locus_tag="PK196_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HON40607.1"
                     /translation="MSKIGSPLFLTAVLLAALVLAAGCSGNGAAPDTGKSAETRTITI
                     TDGLGRTVTVPSPPESVVCSGSGCLRYLVYLQGQDLVVGVDDIEKEDQSVEGRPYALV
                     YGSQFKDLPLIGEFRGKDDPEKILAIDPQVIFKTGSSGSAYSISASEADKLQEKTGIP
                     VVAFPYGSLRNDAEKAEMYTGLSVMGMVLGKEDRAREVVSYIEETIADLENRTADIPP
                     SGQKTVYIGGVSYAGSHGIISTEPAYPPFIWINAKNVAAGLGTAHADVAKEALVDWDP
                     DYLFIDVGTLQMDGGGAIGELKTDRALWGLTAVREGRVYGLLPYNFYSTNYETVLANG
                     YFIGKTLYPDRFEDIDPVEKADEIYTFFVGEPVFGEHNAEYQNMGFAGIPLLIPGLGR
                     SG"
BASE COUNT         3309 a         4713 c         4611 g         2777 t
ORIGIN      
        1 gacggcattc ctccccagtc tgaaggctgg ggtctcctgc ctatacgaag atgaaatggg
       61 atgacttatc agtagaaccg gatacgcgaa cactcacagg gagcgtgata cttatgcccg
      121 gacgaccctc aaccggcgag gactcaacaa tcctccgctg gatgcggttt gagattggca
      181 ggatcaacaa cggcgtggtt gcggaacgca aacgtctctc aaccctgctc tcagaggaga
      241 gaccatcgac catcacaaaa ggaggagagt gttacgaatt cgaccgcggc gtccttctcc
      301 gactgaagga aaccctcccg gctgaactgc agcggcggct caggcttcct gtcctcttct
      361 actttgactc aaccgttcct gacagcctct ttctttcaga tgaggctgcg gtagaggccc
      421 tccagcatct gggggagatc agtccactcc ggcggatgca tgaggggcga gtatggatag
      481 cgaaacccat tgtctatgtc ataatgagtc ggtacccaac ggcaatacag atcgtgatga
      541 ggtagcatcc agtctccaca aaaaatgaag acaaaggatt ccccgatgag agaacgcagc
      601 cggccggatc agccagaacg attggatggc tgccctggat cgctcttctt cgtggctccc
      661 cgccgcagcc gttcgaataa cggccgcgtg atctcatcca tctgccgatc gaagtagtcc
      721 acccattctg caacaagggc aaggtgtctc ttctgttcgg agagccgttc ttccaggtca
      781 ccgacggctt ttcggagttg agcggtctca ctgaccctct cccggcgttc acgatcgagt
      841 tcctcagaga ggcgcgcgat ccggcgctcc tgcagggcaa gcgtatcccg catcaccccg
      901 agatctatga ccacctcggc cgggaggggg gcggcggccg gccctgcctc ttctgccgcc
      961 tcttcttccg gcgcagccgg tttcttcccg gactttagcc tctctgtgat ctcatccggc
     1021 gaaagacctt ttccagatag ttccgcaagt tccctgacga tcttgaccgc tctctgcgga
     1081 aacagcctga ccgggcctat cgtccggtag gtaaagagac tctcgtatgt ctggatgagt
     1141 ctccggacat cctgctccgt gagaccggtg aggtgcgcga tatcaccgat cttgagttcc
     1201 ttctcgctca tgattgccac catccttccg caacccgcct gcgcctccgg gcgggaaccc
     1261 tgcttaaatc tttgtcatca tcaggtatag gtacgtgccg gatcgggttt ttccgtccag
     1321 gcgtcccagt aattcctgca tgaaacggag agaggaggaa agcacccccc gaggagatct
     1381 gaccatcatc cgcaaaaaag tgcgcgccgc ccgcctgcag gtgcagccgg acggaaccct
     1441 gcgcgttgtt gcaccgccgt cgtttgacgt caaaggtttc ctggagcgca acgctgactg
     1501 gatcgagagg cggcaagatg aactagaccg actggccgca gaggggtgcg ggcgggaaga
     1561 tctgctcctc ctccacggac ggttttaccg cctgctcccc ggcgcgcggt tcgcgatcga
     1621 cgtcccgggg gagacggtca cctgcccatc catatggagc ctgaggcaaa acctgagcct
     1681 gctcctgagg gaggagatcg caaaggtggt tgaggcccat accgaccctc tcagccgtaa
     1741 ggctggccgg atcacggtta agatgcagag aacccggtgg gggagctgct cgacactcaa
     1801 caacctgaac ttcaacctcc gcgtaatagc cctcccgaag tcgctgcgcg agtatatcgt
     1861 cgtccacgag gtcgcccacc tgcgcgagca gaaccactcg cagaggttct ggcgtctggt
     1921 cgcagaccgc tgccccgatt acctggaggc ggaagctgaa ctgcggcgat actgggtcat
     1981 actcgagcgg aatcgggtat ggaggaggct gcaggacagc agataaccgg agtgattttt
     2041 cttcgcttcg cgatcggagg ctgccaaacc aataccttca ggcagcgccc aggtccgatg
     2101 aacctctgcc tggactgatt ccaggaaaat ggcggctgcg caagaccgct cgcccctatc
     2161 aggatgcgca gatggactgg tttttctggc gagaggggcc ggtgagatgc ggtgttcttc
     2221 atcggttccc gctgaacccc ctttgatacc tcatccgcga cacctggccc ggtttcacac
     2281 cgccaatcat caggcactgc ccatcttccc ggggggggtg gtgagggtgg ttcaacaaaa
     2341 gaccttcaac acgattggca cgattgcatc accggtagtg tttgcaagaa gagcctgtca
     2401 accatttctg cagggtctcg acccattgac cgcgcttcag ccctccgcct ccggtctctg
     2461 atagtggttc cggtaggtca ggagaagtgt ggcacagatc agaaggaaaa tggaaatgga
     2521 aaccaggaac ccggctcccg gggggatcag gtttacaacc caatcgatgc caagagcgat
     2581 tgcataactc ccgataacag cgcctatgac gatacaggtg agaacctcgg tctttgccat
     2641 cccgagcatc cgcccgacga tggatccaac gatactcccc gaaccgtcga agggaagcgc
     2701 cacaaacgct atgagcccca caaagtagag cctctccagc cagggtcgtc tgtcaagaaa
     2761 cgacctcccg gcctccacaa aacggctgat ccaggggccg acgcgaggta tgcgcatgag
     2821 cagcgggaag ttccaggcca tgaaaagccc gccggcaaag tccagaaaca ctatggtaaa
     2881 cgcagccagc caccagggga tccccccgag gatacagaga ggtatgacag attcgcggcc
     2941 aagcggcggg acaacatagg cgaccatcat cgcgaagaga aagagccacc caccaaccgg
     3001 gagcaagaac cagacgacag agagaaagag accgcagagc aggaacggaa tcaggacttt
     3061 caagacgttc gtccggtcat cggcatcatc atcgccccat atctcaatct gccgcatgaa
     3121 ccgccgtcac ctgcggtgct ctctcgatga cggggtcacc cctccgcatc aggatccccc
     3181 ggggccactc ctataccggg tgttcaggat acgctcgatg atctttgatg aactggccag
     3241 agagccgaaa taaccggcta tccggactac ttcagcatcg agtccacgct cccccaggtt
     3301 ctggcggaga cgctcctcat cgaagtgctg gttaaacccg agcgtgatga tatccggccg
     3361 gatctccgct ataggtctga acatatcatg gaggtcgcca agaatcgcat gatccacagg
     3421 cttcagtgca gcaaccatct ggagtctctg gtcctcgggg atgaccggtt ttggcttgtg
     3481 cttcacgttc gcgtcccgcg ccacgatcac atagagttcg ccgccgagtt tccgggactc
     3541 ctccaggtaa tagaggtgcc ctggatgaag gatatcaaac gtcccggtcg cgaccacacg
     3601 cctcatccaa tcacctccag gtctgtcacg ctcccgtccg cgcggtaaca gcgccagtcg
     3661 gcctgcgtgt agggggggcc gatgatgatg tggtagcggc caaccgaggg gaagaacctg
     3721 agatccgccg gtgacggtga gaggagaccg ttcgggtggc tgtgggcgct gccggcctgg
     3781 tgggtatcca gcgggagcat atcgatgaag aacgaggcgc tcttctcccc gactatggtg
     3841 ccgggcacaa ggtcgagatc gctgatgaca ccgttacgct cccgcagcac cgcaacgaac
     3901 tcgtttggat gatgttgccg gccgagttca aagagcatct caagcagacc cctgctgatc
     3961 ccgcgtatgg ccattcttac agacagttac gaatggaagg aagataagcc ctcgcgttgg
     4021 gcatggataa ccgcgaacca tttcagaacg gcccgtcctg ttcaaaactc cggccaagcc
     4081 tgatggcaag ttccgcctta tagagttctt taccgaggta cgcggcgtgg tcgagtcggg
     4141 agaggctccc gtttgagagg agggcgcaga agacgtcctc ccagcacttc ccccgcacgg
     4201 cgcggccgcg gtgaaccgcg acgattgagt ccccctcgac cccgatccgg atacacccca
     4261 gcggatcata gactacctca tccggcgcag gagacgcctc catgacactc tcgtagtcca
     4321 gcggcggttc gcggcggcgg cgcttctctt taaggatgag gagatcaagc cccaggtctt
     4381 tcggataagg ccggccggtc atgagagcca tcatctcggt cgcccggcgc atctccgcca
     4441 cagaaccctg cgtcttgtcg ctatgctcgc tcgtaaagat gaccgcggcc cccacctcgt
     4501 gcgccatccc ggcaagcaga gcgtttgaac caggggaatc ggcatccagc agttccacga
     4561 cgtttccagc cccgaagaag agcggacgag gggcatcgga aaatctggcg agcgatcctg
     4621 tcagtcccga acccacaggc aggagcaggg ggtcggcgat caggtggcgg acgccgacct
     4681 ccctcgcggc agcaacgttc tccgcgagag ttcgctcacg cggcaccaca accgcggccg
     4741 ctccggcatc cgcaaccgcc ctacctatga ggggtatgtt tccctcatgc agggagagaa
     4801 ccaggtcagc acggttgagc gccgcctcta tgagatccgg atcctgcgtg tccaccgcca
     4861 gcggggcttt aaccccggag agggccgaga aacaccgtct gacatcctct gccgtcgcat
     4921 cgaaaccaaa cccaaggtca acgatatccg ccccggcctc aaagaatcgc cggacgtctg
     4981 ctaaaaggtc gtcacgcatg tgcgcgtcca tgatctctgc aagcaccttc atcctcgaac
     5041 tgccgccgat cttcagaccg cggatgacga aatccggctc cgcctccgcc tcacggtgcg
     5101 ccacctgccg gtaggcctct tcacgccgtc tctctgccag gaactcgtcg gcgggcagag
     5161 tccgggagag cgtgatcgta tcaagcatcg ggaggatcag cgccagatcg gccgcatgtc
     5221 gaggcccgcg gtatacaggg acaccggtcg cgcgttcaac atcggcaaac gatgcggtgc
     5281 acatcccgga gactatcacc atatcgtagt cgccggaggc aagcagcctc cggaggtctc
     5341 ggggagagag gaacgccgca atctcaccgg tggccaccac atccatctcg tggcggccgg
     5401 caaactgctc agccgccgct ttcacaatac cggctgttgc cgttccggta gggaggagaa
     5461 tgcgcataca cttcacttat tattgggagg tgacaaataa cctgatctca tgctgcggtg
     5521 cgatcttcat atccacaccc ggttctccag ggacggagag agcagtgtgg aagaaatcct
     5581 ccggcgggcc gagcagatcg gtcttgatgc catcgccatc acggatcacg acacggtgga
     5641 aggcgccctc tacgccatgg agtgcgacac gccggtgatc gtgatacccg gcaccgaggt
     5701 ctcgacacag cagggccacc tactcgccct gggcgtcacg gaacccatcc ccgcggggcg
     5761 cgacttcttc gagaccgtag ccctggcccg ttcgcggggg gctctgctta tcctcccgca
     5821 cccataccac cgctggcgcc acggggtcgg gaggagactt gcagcaggta tagaggcggt
     5881 tgacgcggtg gaggtcttca acagccgcta cataaccggg tcagcaaacc ggaaagccgc
     5941 cctcatagcc cggaagttcg ggaaacccgg gatcgcgggg agcgacgccc acaacgcccg
     6001 ttatgtcgga ttcggggtca cctatgtcgt ggcagaaccg gatgcggcct ccatcatctc
     6061 cgcgatccgg gaagggcgga cgatggcgag cggcaggatg acaccgctcc acacctacac
     6121 ccgccagtct ctcaaaggtg ctatccgcaa aatcaggcgg acggtatatc gatgataaac
     6181 ctggcactgc ggttctcgta cttcggtgac cgcttctccg gttcgcagat gcagcccggt
     6241 ctccgaacgg tggagggaga gttcatcgag gcctgcaggc gcatccaggt cttcgacgac
     6301 tggcgtgaag cgagattcgc caccgcaggg aggacagacc gcggggtgca tgcccggcgc
     6361 caggtctgtt ctttcctgac ggatctcccc gaacgcgcga tccaggtgct gaaccgcgtc
     6421 ctcccggggg atatctggtg caccggctgg gcagagccgc ctgagggatt tcacccacgc
     6481 tacagcgcca gatcccggac ataccgctac tacttcttcc ccgccccgga gaagatcgcc
     6541 gccatgaatg atgccgcgca ggtcttcata gggcgtcacg acttctcgtc gtttgccagg
     6601 tcaagcggcc gcgaccctga gaggagaatc cttgcggcac gggtctttct ggaggggcgt
     6661 ttcgcagtct tcgaggttac agccgagagt ttcctctgga acatggtccg ccggatggca
     6721 acggtgctcg aagcggtggg gagcggcgag tccgatgccg cggagatcga acgcctgctt
     6781 gcagaaccga ccgaacagcg ggtcgccgct gctcctccat gggggcttat actctgggat
     6841 atcgactacg gtatgacgtt taaccccatc cagatcgatg tgaagagcgg gcgctacctg
     6901 gcctcccgcc tccgttacca cgcagtcatg gcgtgcgccg ccgaccgtct cgcaccggac
     6961 gaccgggcgg tataacgctc aatagagggc tcacgattcc gcctctccac ctctaccggg
     7021 ggagcggggc gtaaaaaccc tcattacaat tgatgacaga tataagcagg aggagtgtaa
     7081 ccaacttgtc agcgattcca aaagaggagt acatctacaa atgcacgtcc gcctgtgcgg
     7141 gctgcagctc ttccctctgt ctgcgctatg tgctcaaagc cgccggccct gactccgtcc
     7201 tggtcgtacc cgcgtgctgc accagcgtca tccaggggat ctatcccgca acggcaatga
     7261 acgtgcccgt ttacaacgta gcgtttgccg cagcagccgc ctgcgcttcc gggatgagcg
     7321 aggcgtttgc agccacagga aaggagacca acgtaatcgt ctacgcaggc gacggcggga
     7381 ccgtcgatat cgggatccag tcgctctccg gggcgttcga gcgggggacc aacttcctct
     7441 acatatgcta cgacaacgaa gcatacggca acaccggaat gcagcgatcg ggctctacac
     7501 ccataggggc gcgcacaacc acgacccctg gcggcaaacc cacgcccaag aaggatctcg
     7561 accggatagt cgaggcccac aacccgccct acatggcgac agcctgcagc gcctaccccc
     7621 tcgacctcta caagaaggtc aggaaagcgc tctcgatcaa gggcccgaag ttcatccaca
     7681 tccttgcacc atgcccgccg ggctggcgtt accccacgga gaagacggtt gagatgggga
     7741 aactcgccgt gaagaccggg gtctgggtac tctacgagcg cgagttcggg aaactcacga
     7801 tcaacggccc ttcaaaggct gcgatgagaa aacccgaacc gctggatgag tatatcaggg
     7861 gtcagggccg gttcaagaac ctgagccccg agaccctcaa cctgatgcgc cagcaggtgg
     7921 agcagaacat ccagcggctt gcccgcgagg aggagggaat atgctgacga tcgcaaccgg
     7981 caacagggcg gtggccgagg cggtgaaagc cgcaaaaccg ggcgtaatcg ccgcataccc
     8041 gatcaccccc cagaccgaga tcgtcgaaca gatcgccgat tacgtcacca ccggcgacct
     8101 cgatagccgc tacatccccg tggagagtga gcactcggcg atggcggcat gcatcggggc
     8161 gagcgtcggc ggcgtacgga cgttcacagc gacgagctcg cacggtctcc tctacatgca
     8221 cgagatgatc cactgggccg cgggagggcg tctgccgatc gtgatggcaa acgtcaaccg
     8281 tgccctggga cccgggtgga acacctgggc ggagcacacc gacgcattct cccagcggga
     8341 caccggctgg ctgcaggtct acgtgggaac cgtgcaggag gcctatgacg ccacgcttat
     8401 ggcgttcagg atcgccgagg acgaacgcgt cctcctcccg gtgatggtca acctggacgg
     8461 gttctcgctc agccacatca cgcagtcgct cgagacagag gagatcggcg atttcctgcc
     8521 gccataccac ctcccgcacg ccatcgacac agagaacccg cgcggttacg gccccatgac
     8581 cgggccggac aactactacc ggttccgatg ggatatcgag cgctcgatgc gcgacgcccg
     8641 tgacgtgatc gcggaggtcg aaagcgagtt tgcagaccga ttcggccgct catacggccc
     8701 caccgaggag taccggtgcg aggatgccga tgtcgtcgtt atcgccctcg gaacgttcgg
     8761 gaaagaggcc gaagtggcgg tcgaccggct tcgcgatgag ggtataagag cgggatcgat
     8821 gcggctgcgc tggttcaggc cgttccccga cctcgatctc tccgggcggg aggtcgtcgt
     8881 aatcgaccgc gactactctt tcggcttcgg cggcgtggtg gcccactcaa tccgggcgaa
     8941 gaccggaatc gagccctaca gcgtgatcgc cggcctcggg ggacaggagg tcacatacaa
     9001 cgatattgcg gattttgtca gaggaagacg tccgggtgag gagacctggt tcggggtgaa
     9061 cgaccatgta tgaggtcagg atccattcac ggggcggaca gggcggtgtc acagccgcaa
     9121 aactcctggc tctcgcggcc ttcagggacg ggaagcacgc aacagcctgc ccgttctacg
     9181 gggcggaacg gcgcggggcg ccggtggtct cgtttgtccg tatcgacgat gaaccgatca
     9241 aggtctacag ccagatccac gaaccggatc tggtcattgt gctcgacgcg agcatcatgg
     9301 acgtggtgga cgtcctggaa gggctgaagg aaggcgggac tgtcctcctg aacagcgccc
     9361 accctgtcga tggttgcagg ggaacctgtt accacgtcga ccttaccggg atcgcgctcg
     9421 cacagaacct ggttgtcgcg ggaagcccga tcctgaacac gccggtgctc ggggcgctcg
     9481 caaagatggg tgtgatcaca ctcccctcgg cgctgcaggc gatacgggag atgtttgacg
     9541 acgaaaggaa tgtgaaggct gctgtggcgg catacgagga gctgaaggta tgagagagag
     9601 acttgcgctc agcagaccgg gcgaagcagc gtctgggaag acaggatcgt ggcggacgtt
     9661 ccggcctgtg gtggataagg aggcctgcaa cgcctgcggg ctatgcgccc agtactgccc
     9721 tgacggggtc atcgacgagg aactgaatat agaccttgaa ttctgcaagg gttgtggtat
     9781 ctgcgcaaac gagtgcccga aacaggccat cgctatggtg agggaggagc gctgacctcc
     9841 ccctcatcat cgatcgggca ttgcgcaata tactccaggt ttaaccgata gaaatgggtg
     9901 tagccgcagt tgcggcaacg ggtcgtgaag tggcctgatc cgatcgcgag atcgtgcggg
     9961 ccggtgacgg cgcaatgttt gcagtatcca tcggtctcta tgctccaggg atcgcagcgc
    10021 ccgatcggcg tagagatgcc ggagatcccg gtgttctcga acctggggat gaagatccgg
    10081 gtcgccccgc atgccgaaca gaccacctgc gcctgcgctg agactgcttt tatgatctga
    10141 tcggcaacct cgccacaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
    10261 ccaggtctac atggccgccg cgcaggcgct gacggatact ctcggctggc cgctcaccat
    10321 catcatgacc cctgacaaaa aaccattctt cgcggccagt tacatcccga aagagagcag
    10381 atacgggatg accggactta tcgatctcat cccccggatc agaaggctct gggagacgcg
    10441 gcggcaggat ctcgagaaca ccggcaacct tctgctggaa gcgctccaga acgccgcccg
    10501 caccccgcaa aacggcccgg agatcacgaa aggtgtcctc gatgaggcat acagcatctt
    10561 cctcaggata tttgacaggg ataacggagg attcgggaac ccgccatggt tccctgcgcc
    10621 gcacaacctc atattcctgc tcaggtattg gagccgaacc ggaaaggagc ccgcgtacat
    10681 gatggccgca aagacgcttt ctgccatgcg gcagggcggg atcttcgacc agatcggtta
    10741 cgggttccac cgctactcga cggatgcgga gtggttcgtc ccgcactttg agaagatgct
    10801 ctacgaccag gcgctcctgg ctctggcata caccgaggcc ttcctggcga gcgggaagga
    10861 agagttcgag cgaaccgcgc gggagaccat cacctatgtc ctgcgggata tgaccgatcc
    10921 tggcggcggt ttctactcgg cagaggatgc tgacagcgag ggcgaggaag gaaggttcta
    10981 cctctggacg aagaacgaga tcctggaaat cctgggtcag gaggacggag aaatgttctc
    11041 caggatcttc ggtgtaaccg ggccggggaa ctacctggaa cagcccggag gaaggagaac
    11101 tggcagaaac atcctccgtc tgcgccgtcc gctttcagcc ctggcagagg agttcgagac
    11161 cacagaggag gaactggctg ggttcgtgga agaggccaga aagaaactct ttgcctctcg
    11221 agagcagcgc gtccacccgg caaaggacga caagatcctt accgactgga acgccctcat
    11281 gatcgctgcc cttgcaagag ccgcacgggt ctttgacgac ccggaatacc tggcagcagc
    11341 agagagggcg accgcgtttc tcctcaacaa cctccggaga ccggacggaa ggctgcttca
    11401 ccgctacaga cagggtgagg ccggtcttgc ggccaccctc gatgactgcg cgttcatgct
    11461 gctggctcta atcgaggact acgaggcctc tttctccccg aaatacctcc agaccgcact
    11521 cgaactcgca aacgacctca tcgaacacta ccaggaccct gacaacggcg gattcttctt
    11581 cgtcgcggac gatgccgaca cccccgtccg ccagaaaccg gtctacgatg gggctacccc
    11641 atccggtaac agcgcggcca tgtacgcact ctatctcctg gggcggatga cggcgaacct
    11701 cgaactcgag gagacggcgg agaggataca tcgcgccttt gccgggttgg tcaccaggtc
    11761 gcccgccgcg tccccatcgt tcctcaccgg ggttgagttg atgaagggag aaaagaccga
    11821 ggtggtcatc gccggggttc cgggagctga ggatacagcg gcaatggtcc ggaagatcta
    11881 ctcgcactac atacccggtt ccgtggtcat actccgcccc gccggtgaag agagaccgga
    11941 gatcgcggag atagccgggt ttacccgtga tatggtgatg atcgatggga gagcgactgc
    12001 gtacgtctgc accaaccacg cctgcaggat ccccacaacc gaccccgacg agatgctgcg
    12061 ggctgcagga accggaggaa tacgccggca ccaaccaggc gtctgagggt ggggcgacga
    12121 tcgagcggta aacccaccgg tcagcaaccg tcgccgggtt ccaggggaac cacgacacgg
    12181 taaccgttgt agcgctcgac tgtcaccgga acgccgtaga ccgcctcgat cgtctccggc
    12241 gtcaccacct ccggcccccc tgcggcatgg attatgccgt ctttcaagag gatgtagcgg
    12301 tcggcgtaac gaagcgccat gttaaggtcg tgcatcgtca tcaccgcagc gacgttgtgc
    12361 tctctcgtca cccgccggat gatccccagg atctcgagct ggttcttgag atcaaggctg
    12421 ctcgtaggct catccaggag aaggagtcgg ggctcctgga caagcgctct tgcgatactc
    12481 accttctgga gttctccacc gctcatctcg tcgatgtagc ggagtgcaag gttctccaga
    12541 tcgagcatcc ttaaagccgc ctcgacgatc cggagatcgc gctccgtcac gtcccagccg
    12601 atgtgcggcc ttctgccgag taggacggca tcgaatgcgg tcatccggcc ggcctcacac
    12661 cgctgcggaa cgtaaccgac cttccgggcg atcgacaacc tgtccgcctt caggatctcc
    12721 gagccctcga ccaggatgga acccaccttc ggccgcagga tggcgttcat acacttcaag
    12781 agcgtcgtct ttccgacgcc gttgggaccc aggatcgcga gcacctggtg cggccgcagg
    12841 tcaaacgtga tatccctgat caccggggaa ctccggtaac tgaacgccac gccatcaacg
    12901 ttgaggatca ccgcctatac ccccgcaaga gcagatagat gaagaccggc gcgcccagaa
    12961 acgccgttaa cacagcaacc gggaggacat aaggggcgat gatgaccctt gcaaccgtgt
    13021 ccgaggcgag taggaggagg ccgcccatca cacaggagcc cgggataagg tagcgctggt
    13081 cgtcaccgat gagccttctc accatgtgcg ggcagacgag ccccacaaac ccgatcaccc
    13141 cgaggaacga gacgatcacg gcggatagga gcgccgcgac aaccatacca atatccctga
    13201 ccgcctcgac gttgacaccg aggcctttcg cggtctcgtc gccggcatcg atagcgttgt
    13261 agttccagcg gttcgcgatg aagtagagag accccgccag ggtgacgaaa gccatgatcc
    13321 cgagttcctg ccatgtggcc cgaccgacgt cgccaaacgt ccagaagacg accgcagaga
    13381 gctgggtatc gtcggcgaag tactggagga acatagtccc ggccgtgaag agggaggaga
    13441 gggcaacccc cgcgagcacc atcacctcgg gcgacgttcc acgtatacgg gagatcagga
    13501 ggatcaccgc ggttgcaagg aggcagaata tgaatgccac caccgttgtc aggtaagggt
    13561 tgctcagtct gacagcgtct gcagccgtgg aatgcatctg cccggtgccg aggaggatga
    13621 ccgagaccgc tgccccgaat gcaccagcgt tcgatatacc aagcgtatac ggtgacccga
    13681 gcgggttgcg gaggatcgac tgcatggcaa ccccggccac cgagagtccg accccggcaa
    13741 cgatcgcggc aagcgcctga ggaagacgga tgttccagac gatccgatcg gtgctggaga
    13801 gatcgagggt gccaccgggg ttgaagagca ggtttatccg gtcgatgctg ccgttcagca
    13861 aggagaggaa gacctcatag ggcgggatgg caaccgcacc gaccgatatc gagactatca
    13921 acaggagggc cagggcgacc acacctccga gtatccagag gcgctttcgc cggacgtagc
    13981 cctggtaatc ggcggggatc accccgtctg caaggtgcat ctattcaacc actcctgccc
    14041 aaacccggta tcaagagtgg gatccccgca aaccccatgt tctgatactc cgcgttatgc
    14101 tctccaaaca ccggttcacc gacgaagaaa gtgtagatct cgtctgcttt ctccaccgga
    14161 tctatatcct caaaccggtc ggggtagagg gtcttaccga tgaagtagcc gtttgccagc
    14221 acggtctcgt agttggtgct gtagaagttg tatgggagga gaccgtagac cctaccctct
    14281 ctcacggcgg taagacccca aagcgcacgg tcggtcttca actcgccgat tgcacccccg
    14341 ccatccatct ggagtgtacc aacgtcgata aagaggtaat cagggtccca atcgacgagc
    14401 gcctccttcg cgacgtcggc gtgagcagtg ccaagtccgg ccgcaacgtt ctttgcgttg
    14461 atccagatga acggcgggta ggcgggttcg gtggagatga tgccgtggct gcctgcgtag
    14521 gagacaccgc ctatgtaaac cgtcttctgc ccggagggcg gaatatcggc tgtccggttc
    14581 tcaaggtcgg ctatcgtctc ctctatgtaa gagacgacct cccgggcgcg atcctccttc
    14641 ccgagaacca tgcccatcac actaaggccg gtatacatct cggccttctc ggcatcattt
    14701 cgaagcgatc cgtaggggaa agccacgacc gggataccgg tcttctcctg gagtttatcg
    14761 gcctctgatg cgctgatcga ataggccgat ccgctggacc ctgtcttgaa gatgacctga
    14821 ggatctatgg caaggatctt ctcgggatcg tccttgcccc ggaactcgcc tatcaggggg
    14881 agatccttga actgcgatcc gtagaccagg gcgtatggac ggccttcgac cgactggtcc
    14941 tctttctcga tatcatcgac accaacaacg aggtcctgac cctgcaggta gaccaggtaa
    15001 cggaggcatc cggagcccga gcagacgaca ctttccggcg gtgacgggac ggtgaccgtc
    15061 cgaccgaggc cgtcggtgat cgtgatagtc cgggtttcag cggatttccc ggtatccggc
    15121 gcggcaccgt taccgctgca cccggcagcg agaacgaggg ctgctaacag tacagccgtg
    15181 aggaagaggg gagaaccgat cttagacata caggtattca gtatagtaac acaatttatt
    15241 atagttatct aatttggatc tattatatat tatcaatttc caataagtgc tacagtatgg
    15301 tcccgtcgtc cggagttctg tgctcccgcc agcggaaaac gacccccacc gggatggata
    15361 actattatga gtaaccgaac caacccctgc aaacagtaga acccggtcat gcccccggca
    15421 cgaccggcgt gttttgaggg atgcagatgc cggagaggat gttaaccgag tttgagggtt
    15481 accagttgct tgaatcatgg ggggtacccg
//
LOCUS       DAOSZX010000029        14906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3387_length_14906_cov_22.225439, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000029 DAOSZX010000000
VERSION     DAOSZX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_14906_cov_22.225439"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="PK196_03955"
     CDS             <1..92
                     /locus_tag="PK196_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HON40608.1"
                     /translation="VPVACVGVDNGENAALLAIRILKAGRAPD"
     gene            complement(189..383)
                     /locus_tag="PK196_03960"
     CDS             complement(189..383)
                     /locus_tag="PK196_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HON40609.1"
                     /translation="MRHPMEYNRGNRNFGGPRNFGGPREMTKTICSDCGKECEVPFKP
                     TEGRPVYCQDCLPKHRKPRF"
     gene            complement(997..1506)
                     /locus_tag="PK196_03965"
     CDS             complement(997..1506)
                     /locus_tag="PK196_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HON40610.1"
                     /translation="MVKRINNTLQFTAEYQRIGTSSPAWQEIRSLVEAAAGCVSLGAV
                     SLENNIPPGVEVFADPLIEKVFTNLIDNALRHGEKVTRISFGLKRGGDFCIIFCEDDG
                     VGVPVDEKEKIFSYAYGKNTGLGLFLAREILSITSITIRETGNPGEGARFEMICPPDM
                     IRAPVSRTG"
     gene            complement(1500..1925)
                     /locus_tag="PK196_03970"
     CDS             complement(1500..1925)
                     /locus_tag="PK196_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HON40611.1"
                     /translation="MEMKQRRSAMNILVVEDSRTQAEFIRQILETAGYNVILAGNGAE
                     AIKQVETARPDIVLTDILMPGIDGYELCRRIKQQNPDLPVIMVTVLFDPADVLKGLAA
                     GADSFIVKPPNPEHVCSQIEAVMRGREMAGAALRMLSRW"
     gene            2315..3418
                     /locus_tag="PK196_03975"
     CDS             2315..3418
                     /locus_tag="PK196_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HON40612.1"
                     /translation="MADVYFANLRARGPGESKIEKIRRLFDAAGFDRVVRHDDLTAIK
                     LHVGERGCDTYLHPLFARQVVNKVKERGAQPFITDTATLYAGSRADAVRHTVTAIEHG
                     FDYSVISAPVIIADGLRGGYWREVAVNGKHFKSVRIAGNILDADSMIVLSHVKGHDLA
                     GFGGAIKNLAMGCAPPSGKAAQHAARPCVDIERCIGCGSCTKVCPQSAMGLSEGVAHL
                     NRERCVGCGDCMRACPEGAIEFDWTTEVRPFIERLCEHALGAVCSRPGRNGYINFLLN
                     ITPDCDCVPWSDAPIVPDIGILASTDPVAIDHASLDLIDSQHGFSHTLLERNLAPGED
                     KFKGVRDYTEGRYQIAYAARIGLGDEAYHLIEV"
     gene            3422..3991
                     /locus_tag="PK196_03980"
     CDS             3422..3991
                     /locus_tag="PK196_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON40613.1"
                     /translation="MSAGKVVLLCGSPRRRGNTELVLGECAKVLEAGGLETETITLAD
                     KQILSCTACGLCSEGECALDDDLNEIIEEIRGADGFIVGAPVYFGTARSDVTAALQRI
                     GMVSMASDSFLSRKVGGPIAVARRGGHTATLQELLMFYLINDMIVPGSTYWNMVFGEA
                     PGEALADEEGMRTVRRFADNVAYLIRKLR"
     gene            4065..4412
                     /locus_tag="PK196_03985"
     CDS             4065..4412
                     /locus_tag="PK196_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HON40614.1"
                     /translation="MDLSSEAIRPDVANTAPLTRREITELLPEVRGWSLKDGRLTSRF
                     GFKGFPEALAFLNRVAEFAARENHFPDLSIQEGRYVEVAWYTYAIGGLSRNDFIMAAR
                     LSERLRNRRGGIV"
     gene            complement(4409..4825)
                     /gene="trxA"
                     /locus_tag="PK196_03990"
     CDS             complement(4409..4825)
                     /gene="trxA"
                     /locus_tag="PK196_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HON40615.1"
                     /translation="MVGERPFDDELQRLREERLRNMEARFTGRSGGVIEISDTTFREA
                     LQKHPALVVDVWAEWCGPCRMVAPVIEDLAHDLAGRVTFGKCNVDENPLIASSFSITA
                     IPTLLFFANGKLVDRVVGALPKEAIKARIMRAFGPG"
     gene            4949..5023
                     /locus_tag="PK196_03995"
     tRNA            4949..5023
                     /locus_tag="PK196_03995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4984..4986,aa:Met,seq:cat)
     gene            complement(5145..6581)
                     /gene="purF"
                     /locus_tag="PK196_04000"
     CDS             complement(5145..6581)
                     /gene="purF"
                     /locus_tag="PK196_04000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HON40616.1"
                     /translation="MCGIVGIVDAGDVSFQLYYALYALQHRGQESAGISTFEGATLYT
                     HKAQGLVAEVFNRHTLQELRGKAGIGHVRYPTTGSKIPENVQPFNFKYRGLTLSIAHN
                     GNLVNTAELREEYERRGQIFCTTTDTEIIGSIIADALRASKSMEDAVLLCMRRLRGSY
                     ATVALLNDTIYAFRDPLGIKPLCIGKLDQGYIVASESVAIDALDGTFIRDVRPGELVR
                     IDESGLSSTQISTANRRAHCIFEYIYFARADSVMDGKLVYDVRRQIGQKLYETSPTEA
                     DLVSPVPDSGTAYAAGYAERSGIPFLEGLMKNRYMGRTFIMPTQKQRDRAVRIKLNTV
                     RGNLKDKRVVLVDDSIVRGTTSRRIVSMIREAGADEVHLRVGSPPIIAPCYLGVDMPT
                     RAELIASGREVEAVRESVGAASLVYIPLEDLVEATGFGELDLCTGCLTGCYPVEIDGE
                     KNSRCVVSYMGGTLQSDLSTFDSGKEET"
     gene            complement(6591..6773)
                     /locus_tag="PK196_04005"
     CDS             complement(6591..6773)
                     /locus_tag="PK196_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HON40617.1"
                     /translation="MSKGTPSMGKRQKHTHIACRRCGKISFHARHKVCSACGFGRSRK
                     IRSYRWTEKKPKVPTH"
     gene            complement(6789..7016)
                     /locus_tag="PK196_04010"
     CDS             complement(6789..7016)
                     /locus_tag="PK196_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HON40618.1"
                     /translation="MTPRPLDILDQVLNRQPVIISLKGGREIRGILQGYDVHMNLVLD
                     RAEEEVDGVTQKLGTLIVRGDNVIYITPSIE"
     gene            7116..7613
                     /locus_tag="PK196_04015"
     CDS             7116..7613
                     /locus_tag="PK196_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HON40619.1"
                     /translation="MSQITVKKRHVIRKSQAAELMKQLGEEIGASADLFRSERIERVE
                     TDAPFEIYLVDKKPLLMGTADWAFPSLRGLVERPIPERRVVVDSGAVRFVVNGADIMR
                     PGIVSISSDVRAGHPVQVVDERHSKPLTVGIALFDAVDMEQQERGKSVKNIHHVGDDL
                     WNLEV"
     gene            7664..8041
                     /gene="sepF"
                     /locus_tag="PK196_04020"
     CDS             7664..8041
                     /gene="sepF"
                     /locus_tag="PK196_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HON40620.1"
                     /translation="MVKNIFDSILGKSPARSEEDYMELDLAAYEVSSEEEPASMYVKI
                     ATIADLKDTPRVKDEVYNGNIVIVDIGRLKMDKVTFERVIKDLRDVAKDVNGDIVGLG
                     EQKYVIITPMSVKISREKIGGGV"
     gene            8042..8569
                     /locus_tag="PK196_04025"
     CDS             8042..8569
                     /locus_tag="PK196_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HON40621.1"
                     /translation="MRESYSGTCPACGGEIQITHHRLEIPHFPDLLLVSIACETCGYR
                     HTDTIILGEGDPVRWTVQVEGPEDLSIRVVRSTTGTIRIPELGMAIEPGTACEGFITN
                     IEGVLSRFEQAVEAILANPENEGEREAALRAKEALAAAREAAFPFTVILEDPAGNSML
                     VSDKAKKAVLEEGET"
     gene            complement(8709..9746)
                     /gene="purM"
                     /locus_tag="PK196_04030"
     CDS             complement(8709..9746)
                     /gene="purM"
                     /locus_tag="PK196_04030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HON40622.1"
                     /translation="MPLYRPLMHEMEGSAVPEHTYRDAGVDIDLEARAVRALIDKLTY
                     RRTGAFSMLGEVGHFAGLIDCGSYVLALAVDGVGTKMLVADALQDWRTVGIDCVAMNV
                     NDLYVMNLEPVAFVDYIATDSLSPEKMAQIGEGLNEGARQANMNIVGGETATLRGLVK
                     GLDLAGTCLGVQKKDLVITGEAIVPGDVIVGLPSSGIHSNGLTLARKIVEECASYDTP
                     LPNGKTLGEDLLTPTRIYAGVLRVTEGCSIHGMCHITGGGLLNLKRLSKYGFSITDPL
                     EPPLIFKWLQEAGSIGKVEAYRTFNMGMGYAFIAPAGSVATIQSIFPDARVVGEVTEE
                     PGVRLKGVEIR"
     gene            complement(9679..11139)
                     /locus_tag="PK196_04035"
     CDS             complement(9679..11139)
                     /locus_tag="PK196_04035"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HON40623.1"
                     /translation="MKFGGSSVGEAACIQRVADIVESHHAAGDEVAVVVSACSGITDR
                     IIAAADEAATSSEEPAIEEFISAMRERHIRLLEATAPDHTREVTEMIDDLLLRLQNIL
                     TAVHTLKELTPRSRDYIISFGERLSAPIVSAALRQRGIPSVVLDGAEAGIVTTANHGD
                     ARALPVSEERIRARVAPLLGESVPVIMGFMGATEQGVITTLGRSGSDYSAAVIGAGIG
                     ADEIWIWTDVDGVMTSDPRIIEDARVLDEISYLEVMELSYFGAKVLHPRSIEPAMQKD
                     IPIRVKNSFRPDAPGTIIRRDRHLEKRVVKAIALIEKVALVNVNGAQMIGRPGVAKMI
                     FEALAEREVNIMMISQGSSEANISLIIDESHLDAALAALNPIVKQGIVREVTHDRDVA
                     ALAVVGAGMAGSPGTGGRIFSALGRAGINVMMISQGSSEVNVSFVVKARDGKRALRVL
                     HDEFRLSENSDETEDAAVSSPHARDGGFGRAGTYLP"
     gene            11212..12843
                     /locus_tag="PK196_04040"
     CDS             11212..12843
                     /locus_tag="PK196_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit A"
                     /protein_id="HON40624.1"
                     /translation="MLVDEIVDAHVLVVGSGGAGIRAAIEASRYGDVVMVSKTIAGKG
                     GCTPMAEGGYNAVLRDQDSIDVHREDTLAGGAYLNDPELVDVLVREAPERMTDLIRWG
                     AVFDVTAECQVAQRPFGGQRFPRTCYAGDRTGHEIIMTLLERLDATDTHIYQEVTVID
                     LVTDENGAVAGAIALDRDGEVMLFRADATVLATGGGTQVYDISTNSAAGTGDGFAMAY
                     RAGAEVIDMEMVQFHPTGAVYPYDARGRLVTEAVRGEGGLLLNARRERFMRRYDPERM
                     ELSTRDVVARAIATEVLEGRGTARGGVYLDVTHLPAETIETRLPVMLEQFLAFGIDIR
                     REPMEVAPTAHHIMGGLRITPECRTTLPGLFACGEVAGGVHGANRLGGNALADTQVFG
                     KRAGEFAGKAAARSGRIDEKSVDEILGMLDGFYDGVINPADVRKDLKLTMWNQAGIFR
                     NAADLRTALMQVRRLSDRRLYAASSDNLAECCTIRNMCTTASLIIRCALLRPESRGAH
                     MRLDADVRTEPAKSPFGHTYISLTREGIERREVAA"
     gene            12840..14303
                     /locus_tag="PK196_04045"
     CDS             12840..14303
                     /locus_tag="PK196_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HON40625.1"
                     /translation="MREIRVRVSRFNPAEDAEPHIEEYTVQVNEGARVLHALHAIHDD
                     LDPTLAYRYCCGAGQCGTCAVRVDGEPVLACMEEARDGMIVEPLALPVLKDLAVDMEP
                     VIAKIARICPRPDGELPPKEVIEEIKPLRDCIECLCCVSACPALLVTEFAGPTVIRQE
                     MRLALDPRDSGDRITDAVKKGLFYCTTCKRCVEVCPKDIDIPGKAIEKLRELANRRGL
                     TLPRHREVARLVQETGRSVERTGVTFLEQVPEVIEPDGPIRGEVGFFVGCMFNGRVPQ
                     TALDAMEVMKRNGIRVIIPHEQVCCGSPLIRTGQTSIVDGLKERNIEAFASRGIETVM
                     TICAGCGATLKNDYETPFVVKDVTEILTEYGIEPPAKLDIRATYHDPCHLLRGQGIRE
                     QPRELLRQAVKEFVEMPVQCCGAGGGVRSGVPEEAMALGAMRGEAVKATGADVVVTVC
                     PFCEFHIADCTDVPVKNLTTILLEGYRKKDAEQEPSG"
BASE COUNT         3160 a         4366 c         4421 g         2959 t
ORIGIN      
        1 gcgtcccggt ggcctgcgtc ggtgtggaca acggggagaa cgccgctctt ctcgcgatcc
       61 ggatcctcaa agccgggcgc gccccggact gaatctttca gcagtgcgcg ggcttgccat
      121 agatcataga gtgatgaggt tagaacgatc cggggaaagg gtgctgatga aaaaggagtg
      181 gggccggttt agaaccgggg ttttctgtgc tttggaaggc agtcctgaca gtaaactggt
      241 cttccttcgg tcggcttgaa aggaacttcg cattcctttc cacagtccga gcagatggtt
      301 ttcgtcattt cacgggggcc gccaaagttc ctgggaccgc cgaaatttct gtttcctctg
      361 ttatattcca ttggatgtct catgcagtta gaaacgctgc atgtacatat ctgaccggat
      421 tgtatataat gcagatgcac cgttcatcaa ccgtttcgcc gacccgtcgt tcccgagacc
      481 atgagcgcgt cctggtagcg cgagtgccaa tgaactccgg gagagaggtg gccgaaaacc
      541 acgcatatgc cggagaaggg gccaggtcaa tgagaggggg tctcgaggtg acgcgcgaac
      601 gtccggtggg gtggcgttcc gtctccctcc ctcaaacagc aattctccat cgatactgtg
      661 cccgtgggat cccctcacca ggttcccggc aagaaacttc cctgaaattt catccagtcc
      721 cggatacggc ttatatggga caatcgccat gcgcggcccg caccttcgaa aggggggatg
      781 acctagacca aagaccttca gcgcgaatga ctcaatagca tcggtgacgg gtctgctcgt
      841 ccctgcgcat actacctgaa aaaagtaaca agtttatatc tcagatcgtt gatttgacga
      901 cccccggaca acgcacgccc gaagtgacgg cattcccggt ttcaggtgct gtcccgcgtt
      961 aaccgcggtt gtggtccccg gatcgaggct cagcgtttac cctgtccttg acaccggtgc
     1021 gcggatcatg tctggagggc agatcatctc aaaccgcgcc ccctcaccgg ggtttccggt
     1081 ctccctgatg gttatacttg ttattgagag gatctcccgc gcaaggaata gaccaagacc
     1141 ggtgtttttg ccgtatgcat aggagaatat cttctccttc tcatcgaccg gaacacccac
     1201 accgtcgtcc tcgcagaaga tgatacagaa atctccaccc cgtttcaggc caaaagagat
     1261 cctggtgacc ttctccccat ggcgcagcgc gttatctatc aggtttgtaa agaccttctc
     1321 aatgaggggg tcggcaaaga cctccacccc cggcgggatg ttgttctcaa gagagaccgc
     1381 gccgagcgag acgcaaccgg ccgccgcctc aacaagagat cggatctcct gccaggcggg
     1441 agaagatgtc ccgatcctct ggtactcggc cgtgaactgg agggtgttgt tgatccgttt
     1501 caccatctcg acagcattct caacgcggcg ccggccatct cccgtccccg catgacggcc
     1561 tcgatctggg agcaaacgtg ttccgggttc ggaggtttga ctatgaaact gtcggcgccg
     1621 gccgcaagcc ccttcaggac gtcggcaggg tcaaagagga cggtgaccat gatgacaggg
     1681 agatccgggt tctgctgctt tatgcggcga cagagttcgt agccatcaat ccccggcatc
     1741 aggatgtcgg tcaggacgat atccggcctc gcggtctcca cctgctttat cgcctcggca
     1801 ccgttgcccg ccagtatgac attgtagcca gcggtctcaa ggatctggcg gataaactcg
     1861 gcctgcgtcc tgctatcctc cacaacaaga atgttcattg cactcctgcg ctgtttcatt
     1921 tccatagcgc ttccctccac catcgctgca ccaactgcca gagtatcctg aaaccctcat
     1981 cctgcctctg acctggcaca ggcggtgtca tccacctgat acgaccgaac tccaggatcc
     2041 ctgtcacctc aggttgaact caatctgaaa tgaccgtatc aaactcatgc tgcttaatat
     2101 ttgtgataaa ggctattttg caggcggatc gcctcgattc catttcccgc gtgaggtaaa
     2161 aaccaccgtc agccgcagca taatgacccc gctgaaccgc acccattcac accttcgcag
     2221 cacggactcc tgaaactccc gttctcgacc gggcacaacg tttatccccg atctctccag
     2281 aacttacgca aagatacgcg ggagaagaaa actaatggca gacgtctatt tcgcaaacct
     2341 cagggcaaga ggcccggggg agagcaagat cgagaagatc cgccgtctct tcgacgcagc
     2401 cgggtttgac cgtgtggtcc ggcacgatga ccttacggcc atcaaactgc atgtcgggga
     2461 gcgtggctgc gacacctacc tgcaccccct cttcgcccgg caggttgtca ataaggtaaa
     2521 ggagcgaggc gcacagccct tcataaccga taccgccaca ctctacgctg gaagcagggc
     2581 cgacgcagtc aggcacaccg tcaccgccat cgagcacggt tttgactact ctgtcatctc
     2641 cgcaccggtg atcatcgccg acggtctccg cggcggatac tggagggagg ttgcggtcaa
     2701 tggaaaacac ttcaaatcgg tcaggattgc cggaaacatc ctggatgctg acagtatgat
     2761 cgtcctctcc cacgtcaaag gccacgatct tgccggattt ggcggggcga tcaagaacct
     2821 tgccatgggg tgcgccccac cgtcaggaaa ggctgcgcaa cacgccgcca gaccctgcgt
     2881 agatatcgag cgctgcatcg gctgcgggag ttgcacgaag gtctgcccgc aatcggcgat
     2941 gggcctctct gaaggggtgg cgcatctcaa ccgtgagcgt tgcgtcggtt gcggcgactg
     3001 catgcgagcc tgccccgagg gagcgatcga gtttgactgg acgaccgaag tccgaccatt
     3061 catcgaacgg ctttgcgagc acgctctcgg cgccgtctgt agcaggccgg gaagaaacgg
     3121 ctacatcaac ttcctcctca acatcacacc ggattgtgac tgtgtcccat ggagcgatgc
     3181 gcctattgtc ccggatatcg ggatcctggc ctccaccgac ccggtcgcga tcgaccacgc
     3241 gagcctcgat ctaatagaca gccagcatgg gttttcgcat acactgcttg agaggaatct
     3301 ggctccaggg gaagacaagt ttaaaggagt ccgggactat acagagggta gataccagat
     3361 cgcctacgcc gccaggatcg gacttggaga cgaggcctac cacctgatcg aggtctgaag
     3421 gatgtcagcc ggaaaagttg tgctgctctg cgggagcccc agacggaggg ggaacaccga
     3481 actcgtgctc ggcgagtgtg caaaggtact cgaagccggg ggtctggaga cggagaccat
     3541 cactcttgca gataaacaga tcctctcctg caccgcctgc gggctctgca gcgaggggga
     3601 atgcgctctc gatgacgact taaacgagat catcgaagag atcagggggg ccgacggctt
     3661 catcgtgggt gcaccggtct atttcggcac cgcccgaagt gatgtcacgg cagccctgca
     3721 gcggataggg atggtctcga tggcctcgga ctctttcctc tcgcggaagg tcggcgggcc
     3781 tatcgccgtg gccaggcgcg gcgggcatac cgcaacgctc caggaactgt tgatgtttta
     3841 cctgatcaac gacatgatcg tgcccggttc aacatactgg aacatggtct tcggggaggc
     3901 gcccggtgag gcgctggccg atgaggaggg gatgagaact gtgcggcgat tcgccgataa
     3961 cgtggcgtac ctgatcagaa aactccggtg agtttgatcg ttatcaccgg aaacggtctc
     4021 gttgcagaac catcagaatc aatcaagttg ggagaataaa cagcatggat ctttcgtctg
     4081 aggcgatcag gccggatgtc gcgaacacgg caccgctgac ccggcgggag atcaccgaac
     4141 tcctgccgga ggtgagaggc tggtcactca aggatgggcg cctcacctcc cgatttggat
     4201 tcaagggatt ccctgaagcc ctggcattcc tgaacagagt tgctgagttt gccgcgcggg
     4261 agaaccactt cccggatctc agcatccagg aggggaggta tgttgaggtc gcctggtaca
     4321 cttacgccat aggcggactc tccaggaacg acttcatcat ggctgcgcgg ctctcagagc
     4381 ggctccgcaa ccggcgtggc gggatagtct aaccgggacc aaacgcacgc atgatccgtg
     4441 ccttaatggc ctctttaggt agagccccga ccacgcggtc aacgagtttc ccgttagcaa
     4501 agaagagcaa tgtcggtatc gctgttatgc tgaaactgga cgcaatgagt gggttttcgt
     4561 cgacattgca cttcccgaat gttaccctgc cggctaaatc gtgcgccaga tcctcgatca
     4621 ccggcgccac catccggcag ggaccgcacc attcagccca gacgtcgacg acaagggcgg
     4681 gatgtttctg tagggcctcc cggaaggtag tatcgctgat ctctatgacg ccaccagatc
     4741 taccggtgaa tcgcgcctcc atgttcctga gacgctcttc ccgcaaacgc tgaagttcat
     4801 cgtcaaacgg tctctctcct accatacagc gaaattatgc cttcagcacc tataaatgat
     4861 tggccatgcg ctgtgtgcgt ggcagagggg taagcctgca aaaggtatat aacagccgag
     4921 aacgaatgtg tatatcccac agtggtgaag cgaggtgggg tagtcaggaa atcccgacgg
     4981 gctcataacc cgtagaccga tggttcaaat ccatccctcg ctatgatcgg ttcttctgcg
     5041 tttcaaaaag gttttattca agcattcttt catcgacccc gacccaaacc gggagatgcc
     5101 tgctgcacgg tttcagggag attacggtta gatagaatga gagactatgt ctcttccttc
     5161 ccggaatcga atgttgagag atcggactgg agagtaccgc ccatataact cacaacgcag
     5221 cgagagttct tctccccatc tatctccaca gggtagcacc ctgtcaggca accggtacag
     5281 aggtccagct cgccaaaccc tgtcgcctcc accaggtctt caagcgggat gtagacaagc
     5341 gatgccgcgc caacgctctc acgaaccgct tccacctccc tgccgcttgc gatcagttca
     5401 gcgcgggtgg gcatgtccac tccgagataa cagggagcga ttataggcgg ggaaccaacc
     5461 cgcaggtgga cctcgtccgc ccccgcctcc ctgatcatgc tcacaatccg ccgggaggtt
     5521 gtcccacgga cgatgctatc atcgacgagg accacacgtt tgtccttcag gttgccacgg
     5581 acggtgttga gcttgatcct gaccgctcga tcccgctgct tttgagtcgg catgatgaac
     5641 gtcctgccca tgtaacggtt cttcatcaac ccctcaagga acggaatccc ggatcgctct
     5701 gcgtagccag cggcataggc agttcctgaa tcgggaaccg gagacacaag atcagcctcg
     5761 gttgggcttg tttcgtagag tttttgcccg atctggcgcc tgacatcgta gaccagtttc
     5821 ccgtccatca ccgagtcggc acgggcgaag tagatgtact cgaagatgca gtgcgccctc
     5881 cggttcgcgg tagagatctg ggtagaagac agccctgatt catcgatgcg gacgagttcg
     5941 ccagggcgca cgtcgcggat gaatgtgccg tccagtgcat cgatcgccac gctctccgaa
     6001 gcgacgatgt agccctggtc gagtttacca atacagagcg gtttgattcc gagcgggtcg
     6061 cgaaaagcgt agatggtatc attcaggagt gcaaccgtgg cgtaagatcc gcgcaaccgg
     6121 cgcatgcata gcaggacggc atcctccatg ctctttgacg cacggagtgc gtcggctatg
     6181 atgctgccta ttatctcggt atcagtggtg gtgcagaaga tctgtccccg gcgctcatac
     6241 tcctcccgga gttcggccgt gttgaccaga ttaccgttat gggcgatcga gagggtcagc
     6301 ccgcggtact tgaagttaaa gggctggacg ttctcaggga tcttcgaccc ggttgtaggg
     6361 tagcgaacgt gcccgattcc ggctttcccc cgcagttcct gcagggtatg ccggttgaag
     6421 acctcggcaa caagtccctg cgccttatgc gtgtagaggg ttgcgccttc aaaagtagag
     6481 atccccgcgc tctcctgccc acggtgctgg agagcgtaga gggcataata caactgaaac
     6541 gagacatcgc cagcatcgac gatgccaacg ataccacaca tgatacaact ctagtgcgtc
     6601 ggtaccttcg gtttcttctc agtccatcga taactgcgga tcttccggct cctgccgaaa
     6661 ccacaggccg aacagacctt gtgtcgcgca tggaacgaga tcttgccgca ccgcctgcag
     6721 gcgatgtggg tatgcttctg cctcttgccc attgaaggtg tgccttttga cattctctct
     6781 caccaggttt attcgattga aggggtaatg tagatcacat tatcaccgcg gacgatcagc
     6841 gtgccgagtt tctgcgtcac accgtccact tcttcttcgg ccctgtccag taccagattc
     6901 atgtgaacat cgtatccctg gaggatcccc cggatctccc ttccaccttt cagactgatg
     6961 atgacgggct gacgattcag tacctgatct aaaatatcca acggtcttgg cgtcatatga
     7021 ttaccctttg ccgtccattc tggagtaata tatttgagtg aggaacctac ttaaatataa
     7081 ccgcagtggc tgcacccacc acgaggtatc gagcgatgtc acagatcacc gtaaagaaac
     7141 gccatgtcat ccggaagtca caggcagccg agcttatgaa gcaactcggc gaggagatcg
     7201 gagcatccgc agacctcttc cggtctgagc ggatcgagcg ggttgaaacc gatgccccat
     7261 tcgagatcta cctggtcgat aagaaaccgc tcctgatggg cacggcagac tgggcctttc
     7321 cgagccttcg ggggctcgta gagcggccga tacccgaacg aagggtagtg gtcgattccg
     7381 gcgcggttag atttgtcgtt aacggcgcgg atatcatgcg ccccgggata gtctcgatct
     7441 cctctgacgt ccgtgcaggg cacccggttc aggtcgtcga tgaaaggcac tccaaaccgc
     7501 ttactgtggg gattgcgctc tttgatgcag tggatatgga acaacaggag agaggtaaat
     7561 ccgttaaaaa catccaccac gtcggggacg atctctggaa cctggaggtc tgaaggcgat
     7621 aataatggat actattaaat aaaactcatt cctcaatttt tctatggtta aaaatatctt
     7681 cgacagcatc ctcggtaaaa gcccggcacg gagtgaggag gactacatgg aactcgatct
     7741 cgccgcctac gaggtatcga gcgaagaaga gccggcatcg atgtacgtca agatcgccac
     7801 catcgccgac ctcaaggata caccccgcgt caaggacgag gtctacaacg gcaacatcgt
     7861 catcgtcgat atcggccgtt tgaagatgga caaggtaacg tttgaacggg tcataaaaga
     7921 cctgcgcgat gtagcaaagg acgttaacgg tgatattgtc ggcctcggcg aacagaagta
     7981 tgtcatcatc acgccgatgt cggtcaagat ctcgcgcgag aagatcggcg gtggcgtctg
     8041 agtgcgggaa tcatactctg gaacctgtcc cgcctgcggc ggtgagatcc agattaccca
     8101 tcaccgcctg gagatcccgc attttccaga cctcctcctg gtctcaatag cgtgcgagac
     8161 ctgcggctac cggcatacag ataccatcat acttggggaa ggcgaccccg tccggtggac
     8221 ggtgcaggtg gaggggcccg aagatttatc catacgggtg gtcaggagca ctacaggaac
     8281 tatcaggata cctgaactcg gcatggcgat cgagcccggc accgcctgcg aagggttcat
     8341 cacaaacatc gaaggcgttc tctcacgatt tgaacaggct gtggaggcga tcctggcaaa
     8401 ccctgagaac gagggcgagc gagaggccgc gctcagggca aaggaggcgc ttgcagccgc
     8461 aagggaggcg gcattcccct tcaccgtcat cctggaggac ccggcaggga acagcatgct
     8521 cgtcagcgat aaggccaaga aggctgtcct ggaagagggg gagacctgac ctttttttcc
     8581 cgcttccaac cctgcaaatc cagtgaagga agagagaggg ttttttcctg ccgcctggcc
     8641 gctgtggccg gtttggggac ccggccaacc tttccccccc gagatccatc aaatgcgaga
     8701 gagaccagtc acctgatctc cacgcccttc aacctcacac ccggctcttc cgtcacctca
     8761 ccgaccaccc gggcatccgg gaatattgac tggattgtgg ccacgctccc ggcaggcgcg
     8821 atgaaagcgt agcccatacc catgttgaac gtccggtacg cctcgacctt cccgatgctc
     8881 ccggcctcct gcagccactt gaatatgagg gggggctcca gggggtcggt gatggagaac
     8941 ccgtattttg agagacgttt caggttcagg aggccgccgc ctgtgatgtg gcacatcccg
     9001 tggatgctac agccctccgt caccctgaga accccggcgt agatccgggt aggggtcagg
     9061 aggtcctcgc ccagggtctt gccgttcggg agaggggtgt cgtaggaagc acattcctcc
     9121 acgatcttcc gggcaagggt caggccgttg ctgtggatgc cgctcgaggg gagaccgact
     9181 atcacatccc cgggaacgat cgcctctccg gttataacaa gatccttctt ctggacgcca
     9241 aggcacgtcc cggcaagatc cagacctttc acgagtcccc gaagggtcgc ggtctcgccg
     9301 ccgactatgt tcatgttagc ctggcgggca ccctcgttca atccctcacc gatctgggcc
     9361 atcttctccg gcgaaagcga atcggtcgcg atgtaatcca cgaacgccac gggctcgagg
     9421 ttcatcacat agaggtcgtt cacgttcatc gcgacacagt cgatcccgac cgtccgccag
     9481 tcctgcagcg cgtcggcaac aagcatcttg gtgccgacac cgtcgaccgc gagcgcaagg
     9541 acgtatgacc cgcagtcgat cagcccggcg aagtgcccga cctccccgag cattgagaac
     9601 gcaccggttc tgcggtaggt gagtttatcg atcagcgccc gcacagcccg ggcctcgaga
     9661 tcgatgtcga caccggcatc acggtaggta tgttccggca cggccgaacc ctccatctcg
     9721 tgcatgaggg gacgatacag cggcatcttc tgtctcatcc gaattttccg ataggcggaa
     9781 ctcatcgtgc agcacccgca gggcgcgctt gccatcccgg gctttcacaa cgaatgaaac
     9841 gttcacctcg cttgaaccct gggagatcat cattacgttg attccggcac ggccgagcgc
     9901 ggagaagatc cgcccgccgg ttccggggga gccggccatc ccggcaccca caaccgcaag
     9961 tgcggccaca tcacggtcat gggtgacctc gcgcacgatc ccctgcttta cgatggggtt
    10021 aagggcggcg agagcggcgt cgagatgcga ctcatcgatg atgagggata tgttcgcctc
    10081 gcttgaaccc tgggagatca tcatgatgtt cacctcccgc tccgcaagcg cttcgaagat
    10141 catctttgcg acccctggcc ggccgatcat ctgggcaccg ttgacattta ccagggccac
    10201 cttctcgatg agggctatgg cttttactac gcgcttctcc aggtgcctgt cccggcggat
    10261 aatggtgcca ggggcatctg gcctgaaaga gttcttgacc cggatgggga tatccttctg
    10321 catcgcgggc tcgatcgacc gtgggtggag cacctttgca ccaaagtagg agagctccat
    10381 cacctccagg taggagatct cgtcgagcac ccgtgcgtcc tcgatgattc ttgggtcaga
    10441 ggtcatcacg ccgtcgacgt ctgtccagat ccagatctcg tcggcaccga tcccggcacc
    10501 gatgacagcg gccgagtagt cagacccgct ccggcccagg gtggtgatga ccccctgctc
    10561 ggtcgccccc atgaacccca tgataacagg aacactctca ccgagcagcg gggctacccg
    10621 ggcacggatt ctctcctcgc tcaccgggag agcacgggca tcgccgtggt ttgcagtcgt
    10681 gacgatcccg gcctcggcgc cgtcaaggac gactgaaggt ataccccgct gcctgagggc
    10741 tgcgctaaca ataggggcag agagtcgttc cccaaaggag atgatgtaat cgcgagagcg
    10801 aggtgtcagc tccttcaggg tgtgaaccgc agtgaggatg ttctgcagcc ggagcagcag
    10861 atcgtcgatc atctccgtca cctcacgggt gtggtccggg gctgttgctt ccaggagcct
    10921 gatgtggcgc tcccgcatcg ctgagatgaa ttcctcgatc gctggctctt cactgctcgt
    10981 tgcggcctca tcggcagccg cgatgatccg gtcggtgatc ccggagcatg ctgataccac
    11041 caccgcaacc tcatctcctg ccgcatggtg tgattcaact atatctgcga cccgctggat
    11101 acatgccgct tccccgacag aactgccgcc aaatttcatc aaaagcctca ttctggctca
    11161 cttatcccct gcaattactt tatgatagta tacaacgcac cgactaataa gatgcttgta
    11221 gacgagattg tggacgcgca cgtgctggtt gtcggaagcg gcggcgctgg tatccgggct
    11281 gccatcgagg cgtcccgcta cggcgatgtg gtcatggtct ccaagactat cgccggtaaa
    11341 ggggggtgca caccgatggc ggagggtggc tacaacgcag tgctgcgcga ccaggactcg
    11401 atcgatgtcc accgcgagga tacgcttgca ggcggggcat acctcaacga ccccgaactc
    11461 gtggacgtcc tggttcgcga ggccccggag cggatgaccg acctcatccg gtggggcgcg
    11521 gtctttgacg tgactgccga gtgtcaggtc gcgcagagac cgttcggtgg ccagcgtttc
    11581 ccccggacgt gctacgccgg cgaccggaca ggtcacgaga tcatcatgac actccttgaa
    11641 cgactggatg caacggatac gcacatctac caggaggtca ccgtaatcga ccttgtaacc
    11701 gatgaaaacg gggcggttgc cggcgcgatc gccctcgatc gggatggaga ggtcatgctc
    11761 tttcgggccg acgcaacggt gcttgcaacc ggcggtggga ctcaggtcta cgacatctcc
    11821 acaaactccg ccgccgggac gggagacggg ttcgccatgg cctaccgcgc cggtgcggag
    11881 gtgatcgata tggagatggt ccagttccac ccgacgggcg cggtctaccc ctacgatgcc
    11941 aggggacgcc ttgtgaccga ggcagtccgg ggagaaggag gtctacttct gaacgcccgg
    12001 cgtgaacggt tcatgagacg ctacgacccg gagcggatgg aactctccac ccgggacgtg
    12061 gttgcaaggg cgatcgcaac cgaggtgctg gaggggcgcg ggacggcccg cggcggggtc
    12121 tacctggacg tgacccacct tccagctgag acgattgaga cacgactccc ggtgatgctc
    12181 gagcagttcc tcgcattcgg cattgatatc aggagggagc cgatggaggt ggcgcccacc
    12241 gcccaccata tcatgggcgg gctccggatc acccctgagt gccggacaac cctgccgggc
    12301 ctcttcgcat gcggggaggt tgccggcggg gtgcatggcg caaaccgcct gggcgggaac
    12361 gcgcttgccg acacacaggt cttcgggaaa cgtgcaggtg agttcgccgg gaaggcagcg
    12421 gcgcggagcg gccggatcga cgagaagagt gtcgatgaga tcctcgggat gctggacggt
    12481 ttctatgacg gggtgataaa cccggcggat gttcggaaag acctgaaact cacgatgtgg
    12541 aaccaggctg gaatctttcg aaatgccgcc gatctccgga cggcccttat gcaggtccgc
    12601 cggctctctg accggcggct ctacgcggca tctagcgaca acctcgctga gtgctgcacc
    12661 atccggaaca tgtgcaccac agcctccctg ataatccgtt gcgcactgtt gcgaccggag
    12721 agccgtggtg cccacatgag gctggacgcc gatgtcagga cggagccggc gaagtcgcct
    12781 tttggacata catacatctc gctcacgcgg gaggggatcg agcggcgtga ggtggcagca
    12841 tgagagagat cagagtcagg gtctcgcgtt tcaacccggc agaggatgcg gaaccgcaca
    12901 ttgaagaata taccgtccag gtcaacgagg gcgcgagggt gctccacgca ctccacgcta
    12961 tccacgacga cctcgacccg acgctagcct accgctactg ctgcggggcc gggcagtgcg
    13021 ggacctgcgc cgtccgcgtc gatggggagc ctgtccttgc ctgtatggag gaggcgcgcg
    13081 acgggatgat agtcgaaccg ctggcgctcc ccgtccttaa agacctggcg gttgatatgg
    13141 agcctgtgat cgctaagatc gccaggatat gcccgagacc ggacggggaa ctcccaccaa
    13201 aagaggttat cgaggagata aaaccgctcc gggactgcat cgagtgtctc tgctgcgtct
    13261 ccgcctgccc ggcgctcctg gtgacggagt tcgccgggcc gacggtgatc cgccaggaga
    13321 tgcggctcgc cctcgacccc agggattccg gcgaccggat caccgatgcc gtcaagaaag
    13381 ggctcttcta ctgcacgacc tgcaagcgct gcgtggaggt ctgcccgaag gatatcgata
    13441 tcccgggcaa agccatcgag aaactccggg aactcgcgaa ccggcgcggg ctgactctcc
    13501 cacgccacag ggaggtggcc cggctggtgc aggagacggg acggagcgtc gagcgaacgg
    13561 gggtcacgtt tcttgagcag gtgccggagg tgatcgagcc cgatgggccg atccgcggcg
    13621 aggttgggtt ctttgtcggg tgcatgttca acggccgggt gccgcagacg gcgcttgacg
    13681 cgatggaggt catgaagcgt aacgggatca gggtgatcat accccacgag caggtctgct
    13741 gcgggtcacc gctaataagg accgggcaga catcgatcgt ggacggcctg aaagagagaa
    13801 acattgaggc attcgccagc cgtgggatag agaccgtgat gacgatctgt gccggctgcg
    13861 gggcgacgct gaagaacgat tatgagaccc cgtttgtggt aaaagacgtc acagagatcc
    13921 tgaccgagta cgggatcgag cccccggcga agctcgatat ccgtgcgacc taccacgatc
    13981 cctgccacct cctccggggc caggggatcc gcgaacagcc gcgggaactg ctccggcagg
    14041 ccgtgaagga gtttgtggag atgccggtgc agtgctgcgg cgccggtggc ggcgtccgtt
    14101 cgggggtgcc ggaggaggcg atggccctcg gggcgatgcg cggtgaggcg gtgaaggcaa
    14161 ccggagcgga cgtggtggtg acggtctgcc cgttctgcga gttccatatc gccgactgca
    14221 ccgatgttcc ggtgaagaac ctgacgacga tcctgcttga gggctaccgg aagaaggacg
    14281 cggagcagga gccatccggg tgagcgattc caccttttca ttacaggcga tcgcgtgcca
    14341 ggagtcgatc cggggcgcct ggccaccccg acctggaagc aaccgaaccg cacagagcca
    14401 aacctccagg gacgattgtg ggcagcagcg gtgcacctgc tgattcacca cacacaaaga
    14461 tgccggttag gttgcagggc aaaacagccg ctcattttca cccctggtga cctgaagaga
    14521 tggggcggca ggtatgagca atatccagag gcatgaacaa tggtggtgac gtctctgcct
    14581 catccggggt ggtaaccacc tcatccgtga gctcctggat acaggcttca ggaggatttg
    14641 ccatgcaccg cccgccatcc cttgaggaga ggggtgggga tacaaaaacc ggacggtgtg
    14701 ggttttctat gaaagccgcc acccactgaa aatcgggtta tcgtgaaaga gctaaaagtc
    14761 ccctattcat tgccgattga gcagtgaggg gaggatatat tgtttgaaaa gataccacaa
    14821 aagaggctgc accctcaggg cgacccggct aaaataatgg gcccggaggg atttgaaccc
    14881 acgaccgctc ggttatgagc cgagcg
//
LOCUS       DAOSZX010000030        14026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3667_length_14026_cov_19.442989, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000030 DAOSZX010000000
VERSION     DAOSZX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_14026_cov_19.442989"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="PK196_04050"
     CDS             <1..1977
                     /locus_tag="PK196_04050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HON40626.1"
                     /translation="EYRPYQREMLEVAASTARSGGIAMIDAPTGSGKSSVVAALLSES
                     GGRKVLVAVRTISQLTTFMRELQLIRQKRGSLKFAYLIGKSSMCPLGGEGDVYRRCEG
                     VKAFSTALMRERAQKGSLVPANDRQIRQQIRKIDPEHPLICPYFIHSKSFTEPEDGGL
                     KMVASRALRVRAERVSTEFIPPEHLAEFCGEVCPYDTMIYAARDADVVLLNFYHLFDD
                     TIREQIYQSLGIEAQDVLLLIDEAHNCGDAVQSIESVTIEERDIMQAGHEISGRARSK
                     PSDAIAQILPRISRFMEALKDSHEAEDWFDPGIFQRMILTGSLYQSTEEIVDDLLKIS
                     EGIREKNLQAGEFRESAIERLTAFFYRILRSASDPAYITVYRKGEDGTVALEVRNIDP
                     STKLQEIAGAHACCVLISGTLSPIESYRRYYFGDLPVTTISLPNAFPPENRRILCAED
                     ITTAYSMRRNTQNLARTEDYITTFAALPGNLAVYFPSYDLLNNFAERCANRIRSKRIY
                     IESRNAQESASMLREFMNLPGTGTSGIIFAVCGGKWSEGLDYRGEMLSGALVIGLPLA
                     PFNRVRRMVIDYFRMKFGEEGEFISYTLPAINRALQALGRVLRTPEDRGMLVLGERRF
                     LEERVHAALPPWMQEEMTPCTIDHFREEVKRWRS"
     gene            1965..2432
                     /locus_tag="PK196_04055"
     CDS             1965..2432
                     /locus_tag="PK196_04055"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HON40627.1"
                     /translation="MAILTGSCRFGLWHVHVSWEGDTVYRVRFARQGIGGAVPEEIRR
                     YCAGRPSRLTDLQSIATAGEGVYPRIYRAVRGIPCGKTAAYSEIAISVGTAPRVVGLA
                     MSRNPTPIVVPCHRVVAKTGIGGFSPDPAIKEALLAMEREGTVCRSEMREINR"
     gene            2458..3372
                     /locus_tag="PK196_04060"
     CDS             2458..3372
                     /locus_tag="PK196_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HON40628.1"
                     /translation="MKVVLLGAGAVGLTIAAKLSRVADVHAVARRRHADAVQQRGFRM
                     TGIWGEGTYKFSCSEDIPPGWQDADYCIITSKSTATEAICRQFADTISGREVVSLQNG
                     IGNEEIIARFTDRVIGAMIITGFEWQGDAAVHVSVEAAPMKLGRFPSGTDPAVERLAA
                     LMREAGIRAEATANIRTDIWSKTLYNCALNPLGALMNVPYGALTNPHAWTIVAAIVRE
                     AYKVAGAEGIVLPWETADAYLDYLREDQLPATAAHHSSMLQDINRKKKTEIDFMNGAV
                     AALADKHGIDAPCNTCIAELIRFREELP"
     gene            3383..3988
                     /locus_tag="PK196_04065"
     CDS             3383..3988
                     /locus_tag="PK196_04065"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HON40629.1"
                     /translation="MRSAIVLVGGAARRAGGREKYFFTFQGRTFIERLLETLQEVADE
                     IVVVARDPDQCERFSHLDGIRCISDVRQGLGPIGGLHAGALAVHGEYVFVAACDMPCI
                     HPGVVNLLFDAAVGYDAAIPCWNADMLEPLHAVYRTKALRDYLGEHDSLSLRPMIWSL
                     KTRYVPVEKIREIDPGLLTFTNINKMEDLESIDPSSDRDSC"
     gene            complement(3998..4762)
                     /locus_tag="PK196_04070"
     CDS             complement(3998..4762)
                     /locus_tag="PK196_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HON40630.1"
                     /translation="MHEEMPVGVSIARIVEETPSIRTFYFDHEISARPGQFVMVWVPG
                     VDEIPMALSSSSSITVQKVGDATAALFAMGEGDVMGIRGPYGNGFSAGGRTLAIAGGV
                     GATPLLPLAESGQVEVFLLGARTASEVLFADRIRRVARLMVATDDGTAGHHGFVTELV
                     SRVDLEGFDRICVCGPEVMMAAVLRILDRTGCAGRGEFSLQRYMKCGVGLCGSCCIDP
                     HGLRVCRDGPVFPGTVLLDSEFGRYARDASGRKQRV"
     gene            complement(4755..5642)
                     /locus_tag="PK196_04075"
     CDS             complement(4755..5642)
                     /locus_tag="PK196_04075"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HON40631.1"
                     /translation="MVVLNPGPIDLGGVGLKNHLILAAGILGTTGASLARLLRCGAGG
                     VVTKSIGPAPMVGHPGPCLVVLEDGVINAMGLPNPSAAFTEELDVLQGEPVIASVFGG
                     TPEEFRTVAGWFTGRVSGIELNLSCPHAEGYGAAIGSNPDIVEECTRAVASLGVPTWV
                     KLTPNVTDITEIGLAAERGGADAIVAINTLKAMRISTSLRRPVLGNRYGGLSGKAIFP
                     VAVRCVYDLYEACSIPIIGCGGVSTADNVIEMMMAGASAVEIGSAVIHDTRIFASIAG
                     DLYSSDGLDAREVVGCAHA"
     gene            5749..6627
                     /locus_tag="PK196_04080"
     CDS             5749..6627
                     /locus_tag="PK196_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HON40632.1"
                     /translation="MTSLAILGAGKVGGETAYLSAVLGLVDEIVLYDVYEPLLRAQVL
                     DLQHTGLDLAISTETKRMRDADIFVFAAGTPRTPEIRTRADLLEANVPVVKRCAELLQ
                     GSPRVIVTVTNPMDANNYGLWRSIGIDRERCIGFGGQLDSARFTGFLHEAGISSQGVV
                     LGEHGEHQVPVFSKTGFEVPVNEREAILERMRGASMEVIRGKGGTVFGPAYHIAMLLD
                     AVIHDRRQVLPCSCVLDGEYGLSGCSLGVPARIGREGVLEIEEWRLDPWESEKMAEAG
                     AFVKQLCRKFDGKSGT"
     gene            6608..9073
                     /locus_tag="PK196_04085"
     CDS             6608..9073
                     /locus_tag="PK196_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HON40633.1"
                     /translation="MANLAREPDKTVLTTLDDFGRVGVGIHQVEYSTGTGFEIPVIHI
                     FGRDLSGTAVRVDVTGFRPYFYAPADQVDGKPLPEGVEVEPGTTYRSIRGDALRRLYT
                     RRPSDVRDVRDKFAPHFEADIPFTTRFMIDCGLTGGVEVPANAARSPDGALVVAYQDL
                     KPADVKAPARVCIMDIECVDERGFPEPERDPVIAVTCWDSFRDDYTTLLWQPGVEPGD
                     APEFRINDRHSVLRYPDEISMFRGLADYIRERDPDILSGWNFVDFDIPYIIRRMAALG
                     LRAEDLARLPGRTERDAIRGRAVFDLLAAYRKMHQAQKESYRLDAIAGEELDVTKVRY
                     GGTVTDLWRSEPAHLVEYNYRDVELCVGIDRKKSIIEFYREIARYVGCPLDRTLNSSN
                     VIDIYVLRKASGRFVLPSKGHAAGDEFEGAVVFEPIIGLRENVVVLDLKSLYPMAMMT
                     INASPETKNPNGELRAPNGVRFSREPDGLTRSILAELLKERDERKRLRDRYPFGSPEY
                     VLYDLQQSVLKVLMNSYYGVSGYPRFRLYDREIGSAVTSVGRAIIRHTRDLITRLGYT
                     VLYGDTDSCMVEMPPGTLEETIARARGIEAQVNASYDEFAKTELNADKHYFSIKFEKV
                     YRRFFQAGRKKRYAGHLVWKEGKEVNEIDVVGFEIRRSDSPQITREVQHTVLEMILRG
                     DSFEEVREYLRDVIRRYRRGEFSLDEAGIPGGIGKSLDSYETEDAHIRGARYSNEFLG
                     ADFKRGSKPKRVYIKAVTEKYPRTDVICFEYGDQVPPEFVVDWETMLEKTLKGPISRI
                     IEPLGWNWEDIDPSRTTLFDFGM"
     gene            complement(9101..9532)
                     /locus_tag="PK196_04090"
     CDS             complement(9101..9532)
                     /locus_tag="PK196_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40634.1"
                     /translation="MGGIKNSTYLDVKKALSRRFSPQDGWQYEWKPLYGNVQPDCLLS
                     RRVAGRTERVVVSVDMAPKVSADAVKKLQEICRGLAAANITVDRAVLVVPAGAAVPEV
                     PAGIEVLETDAWQVVSGRIIWSKNLERNAVLQQELAKRGTA"
     gene            10027..11796
                     /gene="iorA"
                     /locus_tag="PK196_04095"
     CDS             10027..11796
                     /gene="iorA"
                     /locus_tag="PK196_04095"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HON40635.1"
                     /translation="MTIQYLLGNEAIAHACLEAEIDFASGYPGTPSSDVIDTLRVQEE
                     RPLKLEWSTNEKVALENALAAAWCGLRAICTMKHVGLNVAADPLMTSAYTGVTGGLVI
                     LSADDPFAHSSQNEQDTRRYAHFARIPCLDPSSIQEAHDMARDAFALSEEFGLPVILR
                     PTTRICHSKGDVNLGEVGTEHRTAAFHRNPQQYVVIPAHTRPLHKALNAKQPAIRQRL
                     SELGYNRCTVRGRTAVITGGVSAAYVQEVLGDDVSLAIIGAYPIDEDWLREFVDQHNK
                     VLVVEELDPVIEEVVRRVATKTDVYGKMNGTVPYEGEFTPATVVDALAAAGMTASTTF
                     PAVNPVPGVPPRPPILCAGCMHRSTFYAMRRVFRDGIFPSDIGCYTLGLQLGAVDTTI
                     CMGASITVGSGIARSGEKRPVVCTIGDSTFLHTGIPGLLNAVYNGADMVVVILDNRTT
                     AMTGHQPNPNTGVRANGEESTPISLEAISRACGVSWVETVDPYDLPLLLETFRRAKER
                     DGVRVVIAKQPCVMIARRYGIRRKPYTVDPDRCTGCGTCRAFGCPAIAFSDETAVITE
                     LCAGCGVCADICPSGAIILEGRR"
     gene            11793..12380
                     /locus_tag="PK196_04100"
     CDS             11793..12380
                     /locus_tag="PK196_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HON40636.1"
                     /translation="MKPSFDILIVGIGGQGTVLASNVIGEACIIENRSVRSAETHGMA
                     QRGGSVESHVRIDGRYGSLIVPGTADLLMAFDLLEALRYRHYLSADGRLIVNRHLVVP
                     TPVYQQGMEAPDEESILNALADLDLTCIDAAELAEEAGNILSQNIVMLGAASAEIPLR
                     PESLEEAVRRCVPPKTVDVNTTAFRLGRERGAGSS"
     gene            12377..13699
                     /locus_tag="PK196_04105"
     CDS             12377..13699
                     /locus_tag="PK196_04105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON40637.1"
                     /translation="MSGRDAPAALRVDDAGRKRQTAFLEKPRLPGGIERLVESYIEKK
                     TGKPWNDPVVLDRIANAILAQKSRYWKERQVSYRKAYHVLGYLVYHAPVYVVQFEHIL
                     WQIIEQGLAKQHMRILDLGTGPGVVPLAVIDLMGRIGIGTAEVYAVERSEESLEAYNA
                     IVPAAALAAAGERVRVHGPLLADIEALEPAEIPGGIDLMVFSNVLNELRHLEVEQRAD
                     LVAALAGRLSPDGTIVVVEPADLANSTAMRRTVIAIADRGLTVYSPCSFIRGTACSPA
                     RCWTFEQKAAIRPTRLMESLAAREEGYRFVNIDIKYSSALLRKDGKTQHTYRVPPGAK
                     FAPLSHLRQHRDRKINVVGSLISGNLGDARTKVYRVCDGTPADPVYAVIPASLRGPNR
                     DVLDGVAYGDIVQFYGVLVRFNRDHDAYNLVILPATRIEPVRDVSPGG"
BASE COUNT         2770 a         4414 c         4334 g         2508 t
ORIGIN      
        1 gaataccgcc cctaccagcg ggagatgctc gaggtggccg catcaaccgc acgcagcggc
       61 ggcatcgcca tgatcgatgc gccgaccggg agcggcaaat cgagcgttgt cgccgcgctc
      121 ctctcggaga gcggggggcg aaaggtgctc gtcgcggtca ggactatcag tcagcttaca
      181 acattcatgc gcgaactcca gctcatccgg cagaaacgcg gcagcctcaa gtttgcctac
      241 ctgatcggca aatccagcat gtgccctctc ggcggtgaag gtgacgtata ccgccgttgt
      301 gaaggtgtaa aagccttctc gacagcgctg atgcgggaac gggcgcagaa aggctcgctc
      361 gtcccggcaa acgaccgcca gatcaggcag cagatccgca agattgaccc cgaacacccc
      421 ctcatatgcc cttacttcat ccacagcaaa tcgttcaccg aacccgagga cggcgggctc
      481 aagatggtcg cctccagggc gctccgagtc cgggccgaac gggtgagcac cgagtttatc
      541 ccgccagagc accttgcaga gttctgcggc gaagtctgcc cctacgacac catgatctac
      601 gccgcccgtg acgccgacgt ggtgcttcta aacttctacc acctcttcga cgacaccatc
      661 cgtgaacaga tctaccagtc gctcgggatc gaggcgcaag acgtcctcct cctcatcgac
      721 gaggcgcaca actgcggtga cgccgtccag agcatcgaga gcgtgacgat cgaggagcgc
      781 gacataatgc aggccggcca cgagatctcg ggccgggcgc ggtcgaaacc gtcggatgcc
      841 atcgctcaga tcctgccccg gatcagccgg ttcatggagg cgctcaagga ctcgcacgag
      901 gcggaggact ggtttgaccc cggcatcttc cagaggatga tcctcaccgg cagcctctac
      961 cagagcaccg aagagatcgt ggacgacctc ctcaagatca gcgaggggat ccgcgaaaag
     1021 aacctgcagg ccggcgagtt ccgggagagc gcgatcgagc ggctgaccgc gttcttctac
     1081 cggatccttc gttccgcatc cgaccccgcc tacataaccg tctaccggaa gggagaggac
     1141 ggaacggtag cgcttgaagt ccgaaacatc gaccccagca caaaactcca ggagatcgcc
     1201 ggggcgcacg cctgctgcgt cctcatctcc ggcacgctct ccccgatcga gagctaccgc
     1261 cgctactact tcggcgacct ccccgtcaca acgatatccc tccctaacgc cttccccccc
     1321 gagaaccgcc gcatcctctg cgcagaggac ataaccaccg cctactccat gcgccgcaac
     1381 acccagaacc tcgcgcggac agaagactac atcaccacgt ttgcagccct ccccggaaac
     1441 ctggcggtct acttcccctc ctacgacctg ctcaacaact ttgccgaacg ctgcgcaaac
     1501 cggatccgca gcaaaaggat ctacatcgag tccaggaacg cccaggaatc ggcctcaatg
     1561 ctccgggagt tcatgaacct ccccggcacc ggcacctccg gaatcatctt cgccgtctgc
     1621 ggcggcaagt ggagcgaggg tctggactac cgcggcgaga tgctctcggg ggcgctcgtc
     1681 atcggcctac cgctcgcgcc gttcaaccgc gtccgcagga tggtgatcga ctacttcagg
     1741 atgaagtttg gcgaggaggg agagttcatc agttacaccc tccccgcgat caaccgcgcc
     1801 ctccaggcgc tcggccgggt gctccggaca ccggaagaca gggggatgct ggttctcggg
     1861 gagaggcggt tcctcgaaga acgggttcac gccgcactgc cgccctggat gcaggaggag
     1921 atgaccccct gcaccattga ccacttcagg gaggaggtga agagatggcg atcctgaccg
     1981 gatcgtgccg gttcgggctc tggcacgtcc acgtctcctg ggagggcgac acggtctacc
     2041 gcgtgcggtt tgcacgccag gggatcgggg gcgccgtccc cgaagagatc cggcgctact
     2101 gtgccgggag accatcaaga ctcaccgacc ttcagagcat cgcaaccgcg ggcgagggcg
     2161 tgtatccccg gatctaccgc gccgtccggg ggatcccctg cggcaagacc gccgcataca
     2221 gcgagatcgc catctcggtc ggcaccgcgc cccgcgtggt aggtctggcg atgtcacgga
     2281 accccacacc catcgtcgtc ccctgccacc gtgtcgttgc aaagaccgga attggcgggt
     2341 tctcgcccga tcccgcgatc aaggaggcgc tgcttgccat ggagagagag gggactgtct
     2401 gcaggtctga aatgagagaa attaacaggt aacgccgtcc ataatatagt agttaacatg
     2461 aaggtcgtgc tacttggcgc cggggcggtc ggcctgacca ttgccgcaaa gttgtcccgc
     2521 gtcgctgatg ttcatgctgt tgcccgcaga cgacatgcgg acgcggtgca gcagcggggt
     2581 ttccggatga ccgggatatg gggagaggga acttacaagt tcagttgttc ggaagacatc
     2641 cctcccggct ggcaggatgc tgactactgc atcatcacct caaagtccac cgcgacggag
     2701 gctatatgcc gccagttcgc agacaccatc agcggccggg aggtggtgag cctccagaac
     2761 ggtatcggaa acgaggagat catcgccagg ttcaccgacc gcgtcatcgg tgccatgatc
     2821 ataaccgggt ttgagtggca gggcgacgcc gccgtccacg tctccgtgga ggcagcgccc
     2881 atgaaactcg gtcgattccc gtcaggcacc gacccggcgg tagagcggct tgccgccctt
     2941 atgcgggagg cgggcatccg cgcggaggcg accgcgaaca taaggaccga tatctggtca
     3001 aagaccctct acaactgcgc gctcaacccg ctgggtgcgc tcatgaacgt cccctacggg
     3061 gcgctcacca acccgcacgc ctggacgatt gtagccgcca tagtcaggga ggcctacaag
     3121 gttgccgggg ccgagggtat cgtccttccc tgggagacgg ccgacgccta cctggactac
     3181 ctcagagagg atcaactgcc cgcaaccgcc gcccaccact catcgatgct ccaggacatc
     3241 aatcgcaaaa agaagaccga gatcgacttc atgaacggcg cagtggcagc gcttgccgat
     3301 aaacatggta tcgacgcccc ctgcaacaca tgtatcgccg aactgatccg gttcagggaa
     3361 gaattacctt agagggagtt tcatgcggtc tgcgatcgtc cttgtaggcg gggcggcacg
     3421 tcgtgccggc gggagggaga agtacttctt cacgttccag ggcaggacgt tcatcgagcg
     3481 cctcctcgag acgctgcagg aggttgcaga cgagatcgtt gtggttgcgc gcgaccccga
     3541 ccagtgcgaa cggttctccc acctggacgg catcaggtgc atatctgacg tccgacaggg
     3601 gcttggcccc atcggcgggc tgcatgcagg agcgcttgcc gtgcacggcg agtatgtctt
     3661 tgtcgccgcc tgcgacatgc cctgcatcca tccgggcgtt gtgaacctcc tttttgacgc
     3721 cgccgtcgga tacgatgccg ccatcccctg ctggaacgcc gatatgctcg aaccgctcca
     3781 tgcggtctac cggacaaagg cgctgcgcga ctacctgggc gaacacgact ctctatcgct
     3841 tcggccgatg atctggagcc tgaagacacg ctacgtgccg gtggagaaga tccgcgagat
     3901 cgatcccggc ctgctgacgt ttacaaacat caacaagatg gaagacctgg agtcgatcga
     3961 tccttcatcc gatcgcgact cctgttaatc aggagtttta caccctctgc tttctcccgc
     4021 ttgcatcacg ggcgtagcgc ccgaactcgc tatcaagcag tacggtgccc ggaaagacgg
     4081 ggccgtcccg gcagacccgg agaccatggg ggtcgatgca gcacgacccg cagaggccga
     4141 ccccgcactt catgtagcgc tggagggaga actcaccccg ccccgcacac cccgtccggt
     4201 caagtatgcg gaggacggcc gccatcatga cctcggggcc gcagacacag atccggtcaa
     4261 acccctccag gtcaaccctt gagacaagtt ctgtcacaaa cccgtggtgc ccggcggtgc
     4321 cgtcgtcggt tgcaaccatc agcctcgcca cgcggcgtat cctgtcagca aagaggacct
     4381 cggatgccgt tcgtgcgccg aggaggaaga cctcaacctg ccctgactcc gccaggggga
     4441 gcagcggggt ggcgccgacg ccgccggcga tggcaagtgt cctccccccg gcagaaaacc
     4501 cattcccgta tggcccccgg atgcccataa cgtcgccctc gcccatcgca aagagcgcgg
     4561 cggttgcgtc cccaaccttc tggacggtga tcgaggacga agaggagaga gccatcggga
     4621 tctcgtccac gcccggcacc cagaccatca caaactgccc cggccgggcg gagatctcgt
     4681 ggtcaaagta aaaagtcctg attgagggcg tctcctccac aatcctggct atactcaccc
     4741 cgacgggcat ctcctcatgc atgggcgcac cccacaacct cgcgtgcgtc cagaccgtca
     4801 ctgctgtaga ggtcgcccgc gatcgaggca aagatacggg tgtcatggat gaccgcgctc
     4861 cctatctcga ccgcgctcgc cccggccatc atcatctcga tgacgttatc cgcggtagag
     4921 accccgccgc aacctatgat cggtatggag cacgcctcgt agaggtcgta gacgcaccgg
     4981 acggcaaccg ggaatatggc cttccccgag agcccgccgt agcggttccc gagcaccggc
     5041 cgccgcagcg atgtagagat ccgcatcgct ttcagcgtgt tgatggcaac gatcgcgtca
     5101 gcccctcccc gttccgcggc cagcccgatc tcggtgatat cggtgacgtt cggtgtgagt
     5161 ttcacccatg tcggaacccc cagcgaggcc accgcccggg tgcactcctc cacgatgtca
     5221 gggttgctcc cgatcgccgc cccgtatccc tccgcatggg ggcaggagag gttcaactcg
     5281 atcccggaga ccctgccggt gaaccacccg gcaaccgtcc ggaactcttc cggcgtcccg
     5341 ccaaagacgc tcgcgatcac aggttcgccc tgaagaacgt ccagctcctc cgtaaaagcg
     5401 gccgatgggt tcggaagacc catggcgttg atgacaccgt cctcgaggac cacaagacag
     5461 gggccggggt gtcccaccat cggtgccggg ccgatggatt tggtgaccac gccgcctgca
     5521 ccgcaccgga ggagacgggc aagcgatgcc ccggtggtgc caaggatccc ggctgccagg
     5581 atgagatggt tcttcagccc cacgccgccg agatcgatcg ggcctggatt aagcacaacc
     5641 attgaaaaag aggtaggagc tgggtattat tatactctcg cgccaagtgt actggaaagg
     5701 tcttcgtgtc cgctctagtg cacgaagaga tgatcaggat cgatagacat gacctcgctt
     5761 gcaatcctgg gtgcgggaaa agtgggtgga gagacagctt acctctccgc cgtgctcggt
     5821 cttgtggacg agatcgtttt atacgacgta tacgagcccc tccttcgggc gcaggtgctg
     5881 gacctgcagc acaccggtct tgatctcgcc atatcaaccg agacgaagag gatgcgggat
     5941 gccgacatat ttgtatttgc ggcaggaacc ccaagaacgc cggagatcag gacacgggcc
     6001 gacctcctgg aggccaacgt cccggtggtg aaacgctgcg ccgaactcct gcaggggtca
     6061 ccccgggtaa tcgtcacggt cacaaacccg atggacgcga acaattatgg tctctggagg
     6121 tcgatcggga tcgaccgcga acgctgcatc ggttttggcg ggcagctcga cagcgccagg
     6181 ttcacggggt tcctgcatga ggccgggatc agctcacagg gcgtggtgct cggggagcac
     6241 ggggagcacc aggtgccggt cttctcaaag acaggttttg aagtcccggt aaatgagcgg
     6301 gaggcgatcc ttgagcggat gcggggggcg agcatggagg tgatccgcgg caagggcggc
     6361 acagtctttg gacccgccta ccacatcgcg atgctgttgg acgcggttat acacgaccgg
     6421 cgccaggtgc tcccctgctc ctgtgtgctc gatggtgaat acggtctctc cggctgctcg
     6481 ctcggtgtcc cggcaaggat tggccgtgag ggcgtcctcg agatcgagga gtggaggctt
     6541 gacccctggg agtccgaaaa gatggcggag gcaggggcgt ttgtgaagca gctctgcagg
     6601 aagtttgatg gcaaatctgg cacgtgaacc cgataagacc gttctgacca ccctggacga
     6661 cttcgggagg gtcggggtcg gcatccacca ggtggagtac agcaccggga cggggtttga
     6721 gatcccggtt atacatatat tcgggaggga tctctccgga acggccgtca gggttgatgt
     6781 caccgggttc cggccttact tttatgcgcc ggccgatcag gtcgacggca aacccctccc
     6841 ggagggggtc gaggtggagc cggggacgac ctaccgctcc atacgtggag atgcgctgcg
     6901 gcgcctctac accaggcggc catccgacgt ccgtgacgtt cgcgataagt ttgcgccgca
     6961 ctttgaggcc gacataccgt ttaccacccg gttcatgatc gactgcggtc tcaccggggg
     7021 tgtcgaggtt cctgcaaacg ccgctcgatc gccggatggt gccctcgttg tcgcgtatca
     7081 ggacctgaaa ccggcagatg tcaaggctcc cgcccgggtc tgcataatgg atatagagtg
     7141 tgttgacgag cgcggtttcc cggagcccga gcgcgacccg gtcatagctg tgacctgctg
     7201 ggactcgttt agggacgatt acacaaccct cttatggcag ccgggggtgg agcccggcga
     7261 cgcgcccgag ttccggatca atgaccggca cagtgttctc cgctacccgg acgagatctc
     7321 tatgttccgc gggctagccg actacataag ggagcgtgac cctgacatac tctccggctg
     7381 gaacttcgtt gactttgata taccctacat cataaggcgg atggcggcgc ttggtctccg
     7441 ggcggaagac ctggcacgtc tgccggggcg gacggaacgc gacgctatac gggggcgggc
     7501 ggtctttgat ctccttgccg cctaccggaa gatgcaccag gcccagaaag agtcctacag
     7561 gcttgacgcg atcgccgggg aggaactcga tgtaacaaag gtccgctacg gcgggactgt
     7621 aaccgacctc tggcggtcgg agccggcaca ccttgtggag tacaactacc gcgacgtcga
     7681 actctgtgtt gggatcgaca gaaagaaaag catcatcgag ttctaccggg agatcgcacg
     7741 ctacgtcggc tgtccgctcg acaggacact gaactcgtca aacgtcatcg atatatacgt
     7801 cctgcggaag gcgtcaggga ggtttgtcct cccctctaaa gggcatgccg cgggggatga
     7861 gtttgagggg gcggtggtct ttgagccgat catcggtctt cgggagaacg ttgtggttct
     7921 tgatttgaag tctctctacc ctatggcgat gatgaccatc aacgcctcgc ccgagacgaa
     7981 gaacccgaac ggagaactgc gcgccccaaa cggtgtccgg ttctcccgcg aaccggacgg
     8041 gctgacgagg agcatccttg ctgaactctt gaaggagagg gacgagcgca aacgtctccg
     8101 tgaccggtat cccttcgggt cgccggagta cgtgctatac gacctccagc agagcgtcct
     8161 gaaggtgctc atgaactcct actacggcgt ctcgggctat ccccggttca ggttatacga
     8221 tcgcgagatc gggtctgccg tcacgtcggt cggccgggcg atcatccggc acacacggga
     8281 tctcataacc agactgggct acaccgtcct ctacggcgac accgattcat gtatggtgga
     8341 gatgccgcca ggaaccctgg aggagaccat cgcccgtgca agggggatcg aggcgcaggt
     8401 gaacgcgagt tacgatgagt ttgcaaagac cgaactgaac gccgataagc attacttctc
     8461 gatcaagttc gagaaggttt accgccgctt tttccaggcc ggcagaaaga agcggtatgc
     8521 cggccacctg gtctggaagg agggcaaaga ggtcaacgag atcgatgttg tcgggtttga
     8581 gatccggcgc agcgattcgc cgcagatcac acgcgaggtg cagcacaccg tccttgagat
     8641 gatccttcgg ggggatagtt ttgaggaggt gcgggagtac ctccgcgacg tcatcaggag
     8701 gtatcgccgc ggcgagtttt ccctggatga ggcgggtatt ccgggcggga taggaaagag
     8761 cctggatagt tacgagaccg aagatgccca tatccggggt gcgaggtact caaacgagtt
     8821 tctcggggct gattttaagc gcggcagcaa gcccaaacgg gtctacatca aggctgtgac
     8881 ggagaagtac ccgcggacgg acgtgatctg ttttgagtac ggcgaccagg tgccgccgga
     8941 gttcgtcgtc gactgggaga cgatgcttga gaagacgctc aaaggcccca tatcgcggat
     9001 catcgagccg ttggggtgga actgggaaga catcgaccct tcgcggacga cgctctttga
     9061 ttttgggatg tgagaaaagg ggttgagagg ccggggcgcc ttacgccgtg cccctctttg
     9121 caagttcctg ctggaggacg gcgttacgct cgaggttctt tgaccatatg atccggccgc
     9181 tgacgacctg ccaggcgtcc gtctccagga cctcgatccc cgcgggaacc tcgggaacgg
     9241 ccgcacccgc cggtacaacc aggactgccc tgtcgacggt gatattcgcc gcggcaagcc
     9301 cccggcagat ctcctgcagt tttttgacgg catctgcact gacttttggc gccatatcca
     9361 cgctcaccac aacccgctcc gtcctgccgg caacccggcg ggatagcagg cagtcgggct
     9421 gcacgttgcc gtagaggggt ttccactcgt actgccagcc atcctgcgga gagaaccggc
     9481 gggagagtgc cttcttgaca tcaagatacg ttgagttctt tatgccaccc atgtcaatat
     9541 cctcggttta gtgtatggtc acggtcggtt ataagtgtgt agtatctttc tcgacgtttc
     9601 tggctgaacg gcattcgcgg accacccaag cctctgtcac ctgcgtgtca ccgtggcggt
     9661 gtttcaacct ctggaaccac ccccacctcc cggccgctgc tccgcggcct cctcccgccc
     9721 cctcgagagg gggcgggcat tgaggggtga tagccccgga tattgtgcac ggggaagcgc
     9781 agatcgcggc tgagtcttcc cgggggacga accgcggatc ttgctcatcc cccgtggcag
     9841 cgtatcgacg gaaaatcacc ccccccgggg aggggcggaa cgctcccccg cccctgtctc
     9901 gacgtgtcag cgtggcgatc ttttccctgg aaccaccccc taccctccag aaccttcccg
     9961 gagacccgga aggagaccag atcgctatct tcaatgccga ggattcaggt acacttgagt
    10021 ataacaatga caatacaata cctgctagga aacgaggcca ttgctcatgc atgcctggag
    10081 gcagagatcg actttgccag cggttatcca gggacaccgt cttccgacgt tatagacacc
    10141 ctccgggtgc aggaggagcg tcccctcaag ttggagtggt ccacaaacga gaaggtcgcc
    10201 ctcgagaacg cgctcgccgc cgcctggtgc gggctgcgcg cgatctgcac aatgaaacac
    10261 gtcgggttga acgtggcggc cgaccccctg atgacaagcg cctacaccgg cgtgaccggg
    10321 gggctcgtca tcctctccgc ggacgacccc ttcgcgcaca gttcccagaa cgagcaggac
    10381 acccggcggt atgcccattt cgcccggatc ccatgcctgg acccctcatc catacaggag
    10441 gcgcacgaca tggcccgtga cgcctttgcc ctctccgaag agttcgggct gccggtaatc
    10501 ctccggccca caacccgcat ctgccactcc aaaggcgatg tgaacctcgg ggaggtcggc
    10561 acggagcacc ggacggccgc gttccaccgc aaccctcaac agtacgtggt catccccgcc
    10621 cacacccgac cgctgcacaa ggccctgaac gccaaacagc cggccataag acagcggctc
    10681 tcagaactcg gctacaaccg ctgcaccgtc cgggggagga ccgccgtcat cacaggcggc
    10741 gtctcggccg cctatgtcca ggaggtgctc ggcgacgacg tctcgcttgc gatcatcggc
    10801 gcctacccga tcgatgagga ctggctccgc gagtttgtcg accagcacaa caaggtcctc
    10861 gtcgtcgagg agctcgaccc cgtcatagag gaggtcgtcc ggcgggtggc gacgaagaca
    10921 gacgtctacg gcaagatgaa cggcacagtc ccctacgagg gagaattcac cccggcaacg
    10981 gtcgtcgatg cccttgccgc ggccggcatg accgcgtcga cgaccttccc ggccgtcaac
    11041 cccgttccgg gagttccgcc ccggccgccg atcctctgcg ccgggtgcat gcaccgctcg
    11101 acgttttatg cgatgcgccg ggtctttcgc gacggcatat tcccaagcga catagggtgt
    11161 tacaccctgg gcctccagct cggcgccgtg gacacaacca tatgtatggg agcctcgatc
    11221 accgtcggga gcgggatcgc ccgctccggg gagaagcgcc cggtcgtctg caccataggc
    11281 gactcgacgt tccttcacac cgggatcccc gggctcctga acgccgtcta caacggcgcc
    11341 gatatggtcg ttgtcatcct ggacaaccgg accaccgcca tgaccggcca ccagcccaac
    11401 ccaaacacag gggtcagggc caacggcgag gagagcaccc cgatctcgct tgaagcgatc
    11461 tctcgtgcat gcggcgtctc ctgggtcgag acggtcgacc cctacgacct cccgctgctg
    11521 cttgaaacct tccgccgggc aaaagagcgg gacggcgtca gggtggtcat cgcaaaacag
    11581 ccctgtgtca tgattgcacg caggtacggc atccgccgca aaccctatac ggtcgacccc
    11641 gaccggtgca cgggttgcgg cacgtgccgg gctttcggct gcccggcgat cgcgttttca
    11701 gatgagaccg cggtgatcac cgaactctgc gccggttgcg gtgtctgcgc cgatatatgt
    11761 ccgtcgggcg cgatcatact ggagggacgg agatgaagcc atcgtttgat atcctgatcg
    11821 tcgggatcgg cggccagggg acggttctcg cctcaaacgt catcggcgag gcgtgcatca
    11881 tcgagaaccg gagcgtgcgc agcgccgaga cccacggcat ggcgcagcgc ggagggtcgg
    11941 tggagagcca tgtccggatc gacggcagat acgggtctct tatagtgccc gggacggccg
    12001 acctccttat ggccttcgac ctgcttgagg cactccgata ccgccactac ctctctgccg
    12061 acgggaggct tatcgtgaac cggcacctgg tcgtcccgac gccggtctac cagcagggta
    12121 tggaggcccc ggacgaggag agcatactta acgccctcgc tgacctcgat ctcacctgta
    12181 tcgatgcagc ggaactcgca gaggaggcag gaaacatcct ctcccagaac atcgtcatgc
    12241 tcggggcggc gtcggcggag atcccgctca ggccggagag cctggaagag gctgtgcggc
    12301 ggtgcgtgcc gccaaagacg gtagatgtaa acacaaccgc cttccggctg ggacgggaga
    12361 ggggtgcagg atcgtcgtga gtgggcggga tgcccctgcg gcgctcaggg tcgatgatgc
    12421 cgggcgaaaa cgccagacag cgttccttga gaaaccacgc ctccccggcg gcatcgagcg
    12481 gctggtcgag tcatacatag agaagaagac cgggaagccc tggaacgacc cggtggtgct
    12541 cgaccggata gcaaatgcca tccttgccca gaagagccga tactggaagg agcggcaggt
    12601 gagttaccgg aaggcctacc acgtcctcgg ttacctggtc taccatgcgc cggtctacgt
    12661 ggtgcagttt gagcatatcc tgtggcagat catcgagcag gggcttgcaa aacagcatat
    12721 gcgtatcctc gatctcggca ccgggccggg ggtggtgccg ctcgccgtca tcgatctcat
    12781 gggcaggatc ggaatcggga cagcggaggt ctacgctgta gagcgctctg aggagagcct
    12841 ggaggcttac aacgccatcg tcccggccgc cgctctggca gcggcggggg agagggtgcg
    12901 ggtgcatgga cctctactcg ccgatataga ggctcttgag cccgccgaga tccctggcgg
    12961 tatagacctc atggtcttct cgaacgttct gaacgagctc cgtcacctgg aggtcgagca
    13021 gcgtgccgac ctggtcgccg ctcttgcagg ccggctctcg cccgacggga cgatcgttgt
    13081 cgtggaaccc gctgacctcg ccaactcgac cgcgatgcgc cggacggtta tagcgatcgc
    13141 ggatcggggg cttaccgtat acagcccctg ctcgttcatc cgcggaacgg cctgcagccc
    13201 ggcgcggtgc tggacgttcg agcagaaggc cgcgatccgg ccgacccggt tgatggagtc
    13261 cctcgcggcg cgcgaagagg gttacaggtt cgtcaatatc gatatcaagt actcgtctgc
    13321 gctccttcga aaggatggaa agacgcagca cacataccgc gttccgccgg gggcgaagtt
    13381 tgcccctcta tctcatctcc ggcagcaccg cgacaggaag ataaacgtcg tcggttcgct
    13441 tatatcaggg aacctgggcg atgccaggac gaaggtctac agggtctgtg acgggacgcc
    13501 cgcagaccct gtctacgctg tcattcccgc gagcctccgc ggcccgaacc gcgacgtgct
    13561 cgatggagtc gcctatggtg atatcgtcca gttctatggt gttctcgtcc ggttcaaccg
    13621 cgatcatgac gcatacaatc ttgtcatcct gcccgcgacc aggatcgagc cggtcaggga
    13681 tgtgtcgcct ggggggtgat gcggaaatcc cggggggggg aagtcggtcg tcgcaagaag
    13741 ttatcgaatc aacggttctg tgatataaac tccccgtttc ttcccggtat tacgactgcc
    13801 cgctcctggt tgaacggttt acgccgacca acccccgcct cccggccgct cgcgcggcct
    13861 cctcccgccc ccttcatagg ggggcgggca gtaagtggcg atagtccgga taccgtgcac
    13921 gggaaggaaa cgggatttca tggagctttc ggtgagaccg ataagagcct gcctcctaca
    13981 gggcaccgta tcgatgggga atcgcccccg tgggggaggc ggctcg
//
LOCUS       DAOSZX010000031        13765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3760_length_13765_cov_20.821517, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000031 DAOSZX010000000
VERSION     DAOSZX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_13765_cov_20.821517"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1680
                     /gene="thsA"
                     /locus_tag="PK196_04110"
     CDS             79..1680
                     /gene="thsA"
                     /locus_tag="PK196_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HON40638.1"
                     /translation="MLSGQPIIILRENVERTQGFEAQRSNIMAAKAIAAAVRTTLGPR
                     GMDKMLVSSTGDVVITNDGATILHEMSVQHPAAKLAVEVAEAQDDEVGDGTTTATVLL
                     GSLMEEAEDLLAQDVHPTTIAQGYRLGMEKALEIVDELAIPVGADDRENLLKLAGTAM
                     TGKSIEGVKDKVSAIVVDAVRQVATKTEEGIYVVDEDDIKIKKQGGDSMDDVSLIRGV
                     VIDKKRVFEQMPEKVTNAKVALLAQPLEITKTQVKSKIKITSSDQVRAFSEQERESLR
                     KLADQIVAAGANVVLCQKGIADAVQYYLAKHGVYAIEDVKEEDMKFAARALGGNIVNK
                     PEELTEEALGHAEMVEEVPDADLTIISGCENPKSVTILLRGTSQLLLDELERGVYDGT
                     RVIQDAIEDGKFVTGGGSVETELQLRIRDYAATIGGRVQLAIEAFANAFEVIPRTLAE
                     NSGFDTIDKVVAMRKAHAEGARHAGLDVYTGEIVDMLDAGVVEPLRVKVQAIKSATET
                     AVLLIRVDDMMITRSEKRPGEAGAE"
     gene            complement(1682..1885)
                     /locus_tag="PK196_04115"
     CDS             complement(1682..1885)
                     /locus_tag="PK196_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine S protein"
                     /protein_id="HON40639.1"
                     /translation="MLCRFTIIPDGGTLIYSGRPEDTYETALLYFGINPDTALVFHNG
                     RSLPQDKKIEEGEVVLFPTSSRG"
     gene            1937..2021
                     /locus_tag="PK196_04120"
     tRNA            1937..2021
                     /locus_tag="PK196_04120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1971..1973,aa:Ser,seq:cga)
     gene            2196..3023
                     /gene="thiM"
                     /locus_tag="PK196_04125"
     CDS             2196..3023
                     /gene="thiM"
                     /locus_tag="PK196_04125"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HON40640.1"
                     /translation="MTEVSPVQHVDGGIPAGMLAAVREERPLIHHITNSVTINDCANI
                     TICVGAAPVMASAPEEVADMVEAAGALVLNIGTLSAAQVEAMLIAGRHANVLGIPVVL
                     DPVGAGATEFRTASTRRLLEALDIAVLKGNAGEIGVLAETGGSVRGVDSAGIAADPVA
                     TVRECARSTGTVVAMTGETDLVTDGRRVYAVRNGSPLMERLSGTGCMAASITGAFAAV
                     AGDLPVSSAAALAAFGLAGERAEVGARGPYSFRAALFDELAALTPDDLARHARIEVL"
     gene            3024..3656
                     /gene="thiE"
                     /locus_tag="PK196_04130"
     CDS             3024..3656
                     /gene="thiE"
                     /locus_tag="PK196_04130"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HON40641.1"
                     /translation="MGYDLYVITDETVGRGRSHAELATQAVDGGADVIQLRDKRLPAP
                     DLLEAATAIRKITRDSGALFIVNDHLDLALRVGADGVHLGAADIPLPEARSLSPPGFI
                     IGASVGSVAGALRAEAAGADYVALSPTFATGSKSDAGPGFGLFVLSAIRAAVSVPLIA
                     IGGINAGNVADVIAAGADGVAVISAVVGEDDVTAAARDLRARIAAAKQDR"
     gene            3808..4290
                     /gene="tsaA"
                     /locus_tag="PK196_04135"
     CDS             3808..4290
                     /gene="tsaA"
                     /locus_tag="PK196_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HON40642.1"
                     /translation="MTRLEFTPIGIVHTPFHDPRDMPIQPVGARGVRGTVELNPGCAP
                     GLKDIEGFSRIILLYHFHRSEGYSLEVVPFLDARPHGVFATRAPRRPNAIGLSILRLV
                     AVDGAALIVEDVDILDGTPVLDIKPYVPAFDAYPDESSGWLAETAKDPGSVRSDDRFC
                     "
     gene            4402..5217
                     /gene="modA"
                     /locus_tag="PK196_04140"
     CDS             4402..5217
                     /gene="modA"
                     /locus_tag="PK196_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HON40643.1"
                     /translation="MSRKPIALITAVLIAVLLCTAGCSNTVSGNGTSAADNTTLLVYS
                     GAGLKTAMTEIGEVFTEKYGIGVDYNFAGSGTLITQMELSRKGDLFIPGGTPDYRIAQ
                     GKGLVGEPGYVAYHVPVIVVAPGNPKNITSVEDFARPGLKIALGDANTTAIGRAGDKL
                     FQQHGILDAVEANVVLRAPTVNEVAVAMNMGTADAALITFDIVNPETMEVIELPPEEG
                     VVLIVPIGVTTFTEHPDAARLFVEFVTSDEGKAIFVKHGFPAYPDPAYAGIEP"
     gene            5223..5705
                     /gene="tsaA"
                     /locus_tag="PK196_04145"
     CDS             5223..5705
                     /gene="tsaA"
                     /locus_tag="PK196_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HON40644.1"
                     /translation="MEYSCRAIGVARTPFTDPAATPIQAAFSTARGTVEVFPEYRDGL
                     SMLVGFSHILLIYRFHRAAAEELAERPLIDGAEPHGIFATRHFNRPNRLGISVVRLTG
                     IEGGSITIEGVDLLDGTPILDIKPYIPAFDCIESANSGWVTPQHVERIQRQSASFRSG
                     "
     gene            5708..6520
                     /locus_tag="PK196_04150"
     CDS             5708..6520
                     /locus_tag="PK196_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40645.1"
                     /translation="MFQDRAPLPRPTPFKVIFLAVLFCLIAYFLVVIVGLVVYPPFPA
                     LVESLRSREILFAVELSLVTSVVSTALCVVAAVPVAYSLARFSFPGRGLVNTLFNIPL
                     ALPPLVAGVALLIFYGPSTFGKMLSAFGLDIVYTPIGIVVAQFFVNLPYMVRVTRSAF
                     ETINPRFEHVARTLGCTEWGAFRQVTLPLARNGLIAGLVITWSKAIGEFGAVMMLGGA
                     TRMKTETLPIALFLNMSTGDLDLAIAASVILIVISVISFAVFERCSGDRGVF"
     gene            6575..7570
                     /locus_tag="PK196_04155"
     CDS             6575..7570
                     /locus_tag="PK196_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40646.1"
                     /translation="MDLTVADGEYFIILGPTGAGKTILLETLAGIYTPDDGTITLDGR
                     DITRTDPKDRGIGMVYQDYMLFPHLTVGENIGFGLRQRKADPASIREEVQETAALLGI
                     ERLLSRTPGTLSGGEQQRAAIARALVLRPHVLLLDEPLSALDTSTRDRLRRELRSICR
                     ATKTTVIHITHHFEDIFALADRVAVMQEGRVIQTGTPDEVFRKPASEFVAAFTGMENV
                     YCGVSRVLNGEAAIDLGGITVRTVSEIEGEVCVGIRPEEVILSREPFESSAANTFPGR
                     VTEVQRNGTFSRVFVEAGVLFIAVLTPQSVERLRLAEGVAVQVTFKASAVHVFLR"
     gene            complement(7760..7918)
                     /locus_tag="PK196_04160"
     CDS             complement(7760..7918)
                     /locus_tag="PK196_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HON40647.1"
                     /translation="MDSYKCTQCGYVYNPALGDPEHGVKPGTAFEDLPDTWKCPRCGA
                     PKSRFKKL"
     gene            complement(8004..9602)
                     /locus_tag="PK196_04165"
     CDS             complement(8004..9602)
                     /locus_tag="PK196_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HON40648.1"
                     /translation="MELVIGKDGDHDVAVDAQELVTGRTCIIAQSGAGKSWGIAVICE
                     QLLQARIGFCLVDTEGEYSSLAERFPLIRIGSGDDRDVDIERANLRDVMQEAICSRTA
                     VIFDVSETDMRDKVAQLADVLYNLESRLKKPFLLILEEADKFIPQSGDAIKQIEEISR
                     RGRKRGLGLLIATQRPSLVTKNVLSQCNNQIIGKLSIENDLKAVSLFFSSRKEVAELT
                     ELEPGEFFVMGGLSRGKVKMRFRNRICRHGGQTPRLMPAQPVRPGERRRAEPCGGKPQ
                     QEEAEPPMVEPPITRNAVVPVLIREEALDIAREKRKRRFRVLEPEERIVSGEQVYRPL
                     HRVNVRYIGGLLRKTTKTASFIIDGCTGCIVKIDRGLKVRPGFSELLGLDEAAIRVVT
                     GLANGGSTLVEIEAETHLPPAAVKKAIKSLSDAKLITEVKTIGETTVYVPLLTEDVPP
                     LSSLRAGACGLPMEPLQGESLEARVTESSLRTILKGLEPTAEIIEFETIYYPVYVIRF
                     ASERGERSIVLDGCTGKELPFPVS"
     gene            complement(9677..11068)
                     /locus_tag="PK196_04170"
     CDS             complement(9677..11068)
                     /locus_tag="PK196_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40649.1"
                     /translation="MKEVEERTYSRGVILVLVTIASFINPFTASAINLALPVIGTEFS
                     ADAATLAWVSSTYLLASVIFLLPGGRLGDSRGRVRIFLIGIVVYAAGSILSIFTSSMS
                     LLLLFRFVQGVGGAMINATSVALIASLFPPGRRGYAIGINTTAVYAGLSFGPFLGGVL
                     TQFFGWRSIFIVTALLAVPVLFYARKFPAFLNEREMEHFDLPGLALSSALILCLFLGL
                     AWITTHAGLLLLAASLVLGVVFFRVEQHRPDPLLPISLLQKNRVFAASNVAALINYSA
                     TYAVAFLLSLYLQYVRGYEPAAAGTLLLIQPIVQVFVAPAAGRLADRVEPGLLASLGM
                     GVAAVGLFGLSLLNETTPITLVLALLFLVGTGVGLFSSPNTTAIMGSVEKRFYGSASA
                     MVATMRSLGMMLSMGAVLVVFAVIMGSTTVTPGVFPEFMKSLRLLFQVFTALSVFGVF
                     LSLSRNKNRGEGL"
     gene            11166..12362
                     /locus_tag="PK196_04175"
     CDS             11166..12362
                     /locus_tag="PK196_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40650.1"
                     /translation="MTQYMGMRAPHPESQRDLRATSLRLILLLGVASLFGSLVSNGAR
                     SVTGPYLLLLGGSAAVVGLVAGAGEFIGYALRAATGAYLGHNHRYWKVAIGGYCMLAA
                     IPFLAFAGHWATAAALIIAERIGKAIRTPARDTILSHATVAVGRGWGFGFHKALDQVG
                     AVAGPLVMVAALAVTGGYTDGFLVLAIPLIGVAVALFMARSEVPSPRFLEVEEMQGDN
                     IPSILPYAFFIFLGMAGFATFPLISFHLKVQSIVSDAAIPLFYAGAMVVSVFVALLLG
                     RVFDRAGVHIFLSIPIISLATVFLAFSSDPRIAVAGSLVWGAGIGLFEPLLRASIAES
                     TSLERRGKVYGTLSAVYGTAWFLGSAVMGVLYDVSIGHIIAYVVIIEAAALIAYLWLL
                     LIQDPG"
     gene            complement(12408..12746)
                     /locus_tag="PK196_04180"
     CDS             complement(12408..12746)
                     /locus_tag="PK196_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HON40651.1"
                     /translation="MADNFWTGVIVGWLVGVLLGFLLPVLGPLAGGFVAGWIARGGIW
                     NGAKAGLLAGLLGAIVISLLILIGGTVLLGAFGFIAGLGASILIVLAAFIYQGILSLI
                     GGAIGGALHH"
     gene            13004..13428
                     /locus_tag="PK196_04185"
                     /pseudo
     CDS             13004..13428
                     /locus_tag="PK196_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13463..13678
                     /locus_tag="PK196_04190"
     CDS             13463..13678
                     /locus_tag="PK196_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40652.1"
                     /translation="MLIESSGIDLETRKRPHLEYEGAYYYTLVSIPGHGQKGVIGMNE
                     GPDSGSLLKTVGIGAVASGMKGIKEVT"
BASE COUNT         2711 a         4072 c         4162 g         2820 t
ORIGIN      
        1 tgttgcatgc agcagccgcc atcgactgat ttatatagaa cctctgaaca aacctaacgg
       61 acaaaggagg attatacaat gctttctgga cagcccatca ttattttgcg ggagaatgtt
      121 gagcgcaccc agggatttga ggcgcaaaga tcgaacatca tggccgcaaa ggcaattgct
      181 gctgccgttc ggacaacgct cggtccccgg gggatggata agatgctggt cagttcaacc
      241 ggcgatgttg tgatcacaaa cgacggcgca accatcctgc acgagatgtc ggtgcagcac
      301 cctgctgcca agctggccgt cgaggttgca gaggcccagg acgacgaggt cggcgacggc
      361 accacgaccg caacagtgct cctcggctcc cttatggagg aggcggagga tctgctggct
      421 caggatgttc accctaccac catagcgcag ggttacaggc tgggcatgga gaaggcgctc
      481 gagatcgtgg atgagctggc gatccccgtc ggcgccgacg accgggagaa tctcctcaaa
      541 ctcgccggga ccgccatgac cggcaagtct attgaggggg taaaggacaa ggtctccgcg
      601 atcgtggtgg acgcggtgcg ccaggttgcc acaaagacag aagagggcat ctatgtcgtg
      661 gatgaggacg acatcaagat caagaaacag ggcggggact cgatggatga tgtcagcctg
      721 atcaggggtg tcgtcatcga caagaagcgt gtctttgagc agatgcccga gaaggtcact
      781 aacgcgaagg tagcccttct tgcccagccg cttgagatca caaagaccca ggtgaagtcc
      841 aagatcaaga tcacctcctc cgaccaggtc agggcgtttt cagagcagga gcgcgagtcg
      901 ctcaggaaac ttgctgacca gatcgttgct gccggtgcaa acgttgtcct ctgccagaag
      961 gggatcgccg acgcggttca gtactacctg gcgaagcacg gtgtctacgc tatagaggac
     1021 gtgaaagagg aagacatgaa gttcgccgcc agggcgctcg gcggaaacat cgtcaacaaa
     1081 cccgaggaac tcaccgaaga ggcgctcggc cacgctgaga tggtagaaga ggttcccgat
     1141 gccgacctca cgatcatctc tggctgtgag aacccgaaat cggtcacgat cctcctccgc
     1201 ggcacctccc agctgcttct ggacgaactt gagcgcgggg tctatgatgg aacccgggtt
     1261 atccaggacg ccatcgagga cggcaagttt gtcactggcg gggggtcggt tgagaccgaa
     1321 ctccagctgc gtatccgcga ctacgccgca accatcggcg ggcgtgtcca gcttgccatc
     1381 gaggcgtttg caaacgcgtt cgaggtcatc ccgaggacgc ttgcggagaa ttccgggttt
     1441 gacaccatcg acaaggtcgt cgccatgagg aaggcgcatg ccgaaggcgc caggcacgcc
     1501 ggactggatg tctacaccgg tgagatcgtc gacatgctgg atgccggggt agtcgagcct
     1561 ctccgcgtga aggtccaggc gataaagagc gcgaccgaga cggcagtgct gctcatacgc
     1621 gtggacgaca tgatgatcac ccggtcggaa aaaaggccgg gcgaagcggg cgccgagtaa
     1681 ctcagcccct tgaggaggtc gggaacagca ccacctcccc ctcctcgatc tttttgtcct
     1741 gaggaaggct tcttccgtta tggaacacga gtgcggtatc ggggttgatc ccgaaatata
     1801 ggagggcggt ctcgtaagta tcttcgggcc tgccgctgta aatcagcgta ccgccgtcag
     1861 ggatgatggt gaatcggcag agcatatctc gataatgtta tagtgggaga atctaaatgt
     1921 tataggtcga ttttgtgccg aggtagtcta gtccgggaag gcggtggcct cgaaagccac
     1981 tggtgcctgg cacctcggga gttcaaatct ccccctcggc gttcttgcta taacaccccc
     2041 acacgccacg tgatcactcc tgaggtggtg ctgcttctca agaaagccgt gcccgcgccg
     2101 ggcgcatttt tttcgtggag attcctattt cgggtggcag agcctgattt atgccatcgg
     2161 tgcatacctc tactgcagta gttatgtctg gcagaatgac tgaagtttca ccggtacagc
     2221 atgtggatgg cgggatcccc gccgggatgc tcgccgccgt gcgggaagag cggcctctca
     2281 tccaccatat cacaaacagc gtcacaatca acgattgcgc gaacataacc atctgcgttg
     2341 gggccgcccc cgtgatggca tctgcgcctg aggaggtggc cgatatggtt gaagccgccg
     2401 gtgcgctcgt cctgaacatc gggacactct ctgccgcaca ggtcgaggcg atgctgattg
     2461 ccggccgcca tgcaaacgtt ctcgggatcc cggtcgtcct agacccggtc ggtgccgggg
     2521 caacggagtt tcggactgca tccacaagac ggctcctgga ggctctcgat atcgccgtcc
     2581 tgaaaggcaa cgcgggggag atcggtgtcc tcgccgaaac cggcgggagc gtcagagggg
     2641 tcgattcggc cggtatcgcg gccgaccccg tggcgacggt gcgggagtgc gcccgctcga
     2701 ccgggacggt ggtggcgatg acaggggaga cggatctcgt caccgatggg aggagggtgt
     2761 atgccgtcag aaacggcagc cccctgatgg aaaggttatc cgggacgggg tgcatggccg
     2821 catcgatcac cggggcgttc gccgcggtcg caggtgatct tccagtctcg tcggcggcag
     2881 cgcttgcggc gttcggcctg gccggtgagc gggcggaggt cggggcacgc gggccttact
     2941 cgtttcgggc ggcgctcttc gatgaacttg ccgcgctcac cccggatgac cttgcccgcc
     3001 atgcacggat agaggtgttg tagatgggct acgacctcta cgtgatcacg gacgagacgg
     3061 ttggtcgcgg gcgctcacac gccgaactcg ccactcaggc cgttgacggg ggtgcggacg
     3121 tgatccagct ccgcgacaag cggttgccgg cgcctgacct cctcgaggct gctactgcca
     3181 tccggaagat cacccgcgat tccggcgccc tcttcatcgt gaacgaccac cttgatctgg
     3241 cgctcagggt cggtgccgac ggcgtccacc tgggtgcagc cgacatcccg ctgcctgagg
     3301 cacggagcct ttctcctcct ggattcatta tcggggcgtc ggtgggttcg gttgccggtg
     3361 cgctccgtgc ggaagcggcg ggtgccgatt atgtggctct cagcccgacg tttgccaccg
     3421 gttcaaaatc tgatgcgggg ccgggtttcg ggcttttcgt gctctcggcg atcagggcag
     3481 cggtctccgt cccgctaatt gcgatcggcg ggatcaatgc gggaaacgtt gccgatgtca
     3541 ttgccgcagg ggctgatggt gttgcggtga tctcggcggt cgtcggggag gacgatgtca
     3601 cggcagctgc ccgtgacctc cgcgcccgga tcgccgccgc gaaacaggat cgctgagcgt
     3661 cctcagcacc ggctcacacc gcggcgttat ctaagatacc tgcacgaccg cctgaatgtc
     3721 ttcgccgcat gacatccgtc ttcagaagcc atgccggcac acaaaccctc tttgtcctgg
     3781 ctgcagaacc tttcctgcgt gatacttatg acccggctcg agttcactcc aatcggcatc
     3841 gtccacaccc cattccacga cccgcgtgat atgcccatcc agccagtcgg agcgaggggt
     3901 gtccggggaa cggttgaact caaccccggc tgcgccccgg gcctaaagga cattgagggt
     3961 ttttcacgga tcatcctcct ctaccacttc caccgttctg agggctattc gcttgaggtg
     4021 gtgccgttcc tggatgcaag acctcatggt gtctttgcga ccagggcacc ccgccgcccg
     4081 aacgcgatcg gtctctcgat tctgcggctt gttgctgtcg atggcgcggc gctcatcgtc
     4141 gaggacgtcg atatcctgga cggcactcca gtccttgaca taaagcctta cgtcccggcg
     4201 tttgacgcct accctgacga gagttccggg tggctggcag agactgcaaa agatcccggc
     4261 agcgtgcgtt cggatgatag gttctgttga gtgcatggat cggaatattt aacataaccg
     4321 gcgtaaagta aaccagcttg acataacctg aaagtaaatg ggtagtcttt aatagccggg
     4381 gcgccggtac tgaatgatat catgtcaaga aaacccattg cattgattac ggctgtcctc
     4441 attgccgttc tactctgcac ggcagggtgc agcaacacag tcagcgggaa tgggacttcc
     4501 gccgccgaca acacgacgct gctggtctac tcaggcgcag ggctcaagac ggcgatgacc
     4561 gagatcgggg aggtcttcac cgagaaatac ggcatcggcg tcgattacaa ctttgccggt
     4621 tccggcacgc tcatcacaca gatggagtta tcccggaaag gcgatctctt catccccggc
     4681 ggaacgcctg actaccggat cgcccagggt aaggggctgg tcggggaacc cggatacgtt
     4741 gcctaccacg tccccgtgat cgtggttgcc cctggaaacc cgaagaacat cacctcggtt
     4801 gaggacttcg cccggcccgg gctgaagatc gccctcggcg acgcgaacac caccgcaatc
     4861 ggcagggccg gcgataaact cttccagcag catggcatcc ttgacgccgt tgaggcaaat
     4921 gtcgttctac gggcgcctac ggtcaacgag gtggccgttg cgatgaacat gggcaccgct
     4981 gatgccgccc ttatcacgtt tgatatagtc aacccggaga ccatggaggt catcgaactg
     5041 ccgccggaag agggggttgt ccttatagtg ccgatagggg tgaccacgtt cacagagcac
     5101 ccggatgccg cccgtctctt cgttgagttt gtcacttccg atgaggggaa ggctatattt
     5161 gtaaagcacg ggttcccggc atatcctgat cccgcctacg ccggtataga accgtgatcg
     5221 tcatggagta ctcctgcagg gcgatcggcg tcgcacggac acccttcacc gatccggccg
     5281 ccaccccgat ccaggcggct ttctccaccg cccgcggcac ggtagaggtc ttcccggagt
     5341 acagggacgg gctatcgatg ctcgtggggt tctcccatat cctcctcatc taccggttcc
     5401 accgggctgc cgccgaagaa cttgccgaac gaccgcttat cgatggcgct gagccgcacg
     5461 gcatctttgc cacccggcac ttcaaccgcc cgaaccggct tggtatatcg gttgtcagac
     5521 tcaccgggat cgaggggggg agcatcacca ttgagggcgt cgacctcctg gacggtactc
     5581 cgatcctcga catcaagccg tatatcccgg cgtttgactg catcgagagc gcgaactccg
     5641 ggtgggtgac gcctcagcac gtcgagcgga tccagagaca gagtgcatca ttcaggtcag
     5701 ggtgaacgtg ttccaggatc gggcccctct ccctcgcccc acacccttca aggtgatctt
     5761 tcttgccgta ttgttctgcc tgattgcata ctttctcgtt gtaattgttg gtctggtggt
     5821 ctacccgccg tttccggctc tcgttgagag cctgcgatcc cgggagatcc tgtttgctgt
     5881 tgaactctcg ctcgtcacgt cggtggtctc caccgccctc tgcgttgtcg ccgccgtgcc
     5941 tgttgcctac tcgttagctc ggttctcgtt ccccggtaga gggctggtaa acaccctctt
     6001 taacatcccg cttgcactgc cgccgctcgt cgcgggtgtg gcgttgctca tcttctacgg
     6061 cccatcgacg ttcggcaaga tgctctcggc gttcggtctc gatatagtct acaccccgat
     6121 cgggatcgtt gttgcccagt tctttgtaaa cctgccatat atggtaaggg tcacccgttc
     6181 ggccttcgag acgatcaacc cccgcttcga gcacgttgcc cggacgctcg ggtgcaccga
     6241 gtggggcgcg ttccgtcagg tcaccctccc tctcgccaga aacggtctga tagccgggct
     6301 ggtaatcacc tggtcgaagg ctatcggtga gtttggagct gtcatgatgc ttgggggagc
     6361 tacacggatg aagaccgaga ccctgcccat tgcgctcttc ctgaacatgt caacgggcga
     6421 cctcgatctt gccatagccg cgtctgtcat cctgatagtc atatcggtga tctcgtttgc
     6481 ggtcttcgaa cgctgcagcg gcgatcgggg ggtgttctag gttctcaaga tcacggggct
     6541 caccaaacgt ctcggggact ttgttctcga cggtgtggac ctgacagtcg ccgacggtga
     6601 gtacttcatc atcctcgggc ctaccggcgc aggaaagacc atacttctcg agacgctggc
     6661 ggggatatac acacctgatg atggaacgat caccctggat ggccgtgaca tcacccgcac
     6721 cgacccgaaa gatcggggga tcggcatggt ttaccaggat tacatgctct ttccccacct
     6781 caccgtcgga gagaatatcg ggtttggtct tcggcagagg aaggccgatc ccgccagcat
     6841 aagggaggag gtgcaggaga ccgccgccct tctcgggatt gagcgcctcc tctcccgcac
     6901 cccggggacg ctctcaggcg gtgaacagca gcgggcggcg atcgccaggg cgctcgttct
     6961 caggcctcac gtcctcctcc tggacgaacc gctctcggcg ctcgatacct ccacccggga
     7021 tcggctccgc agggagttga ggtcgatctg ccgggcgacg aaaacaacgg tcatccacat
     7081 cacccatcac ttcgaggata tattcgccct cgccgatcgt gtagcggtga tgcaggaggg
     7141 gagagttatc cagaccggca cgccggacga ggtcttccgg aaacctgcca gcgagtttgt
     7201 tgccgctttc accggtatgg agaacgtata ctgcggggtt tcaagggttc tgaacggcga
     7261 ggcggcgatc gacctcggcg ggatcaccgt ccggacggtc agcgagatcg agggtgaagt
     7321 ctgtgtcggt atccggccgg aagaggtgat tttgtcccgc gaaccttttg agtcaagcgc
     7381 tgcaaacacc ttccccggca gggtgacaga ggtccagagg aacggcacgt tttcaagggt
     7441 cttcgtcgag gcaggggttc tattcattgc cgtcctgaca ccgcagagcg ttgagcgcct
     7501 gcggctggcc gagggtgtag cggtccaagt cacattcaag gcttcggccg tccacgtctt
     7561 tctgcggtaa gagcaggtca ttcatcccgg ccagaaggag cggccggatg gggaagaatc
     7621 caatgagata acagaacgaa cgtcttctgc ggctgaccat ccgcgccacc cggccgaagg
     7681 gaaggggcgg aaacgccccc cccgtgtcct gacatgctcc agaaaaagca gaatagaggc
     7741 cgggatgtgc ccaccgcact tataacttct taaaccggct cttcggcgcg ccgcaccggg
     7801 gacatttcca ggtatctggc aggtcttcaa aggccgtgcc aggtttaaca ccatgttcag
     7861 ggtcgccgag agcggggttg tagacgtagc cgcactgtgt gcacttgtat gaatccattg
     7921 ttcactcacc tcattccctg ccggccgccg ggacggcaag gggtaatatc tcgtttactt
     7981 ccgcttcagg ataagtgctc cggtcacgat accgggaaag gaagttcctt tcccgtgcaa
     8041 ccgtcgagga cgatcgagcg ctcaccgcgt tccgatgcaa accggatcac gtagaccggg
     8101 taatagatcg tctcaaactc gataatctcg gccgttggct caagcccttt caggatcgtc
     8161 cggagcgacg actccgtgac ccttgcctcc aggctctcac cctgaagagg ctccatgggg
     8221 aggccgcagg ctccagcccg gagagaagag agcggcggga cgtcctctgt caggagcggt
     8281 acgtagaccg tggtctcgcc gattgtcttt acctctgtta tgagtttcgc atccgaaaga
     8341 ctctttatcg ctttcttcac cgcggccggc ggaagatggg tctcggcctc gatctcgacg
     8401 agcgttgagc ctccgtttgc aagcccggta acgaccctga tcgcggcctc atcgagcccc
     8461 aggagttcag agaaccccgg cctgaccttc agtcccctgt caatcttgac gatgcagccg
     8521 gtgcagccat ctatgatgaa cgacgctgtc tttgtggtct tccgcagaag tccaccgatg
     8581 tagcggacat tcacccgatg tagcggccgg tagacctgct cacccgatac tatccgctct
     8641 tcgggttcca ggaccctgaa cctgcgtttt ctcttctccc tggcgatgtc gagcgcctcc
     8701 tcacggatca gcaccgggac aaccgcgttc ctcgtgatgg ggggttccac catgggcggt
     8761 tctgcttcct cctgctgcgg tttgccgccg cagggttcag cccggcgccg ttctcccggc
     8821 cggaccggct gcgccggcat gagtcttggc gtctgcccgc cgtgcctgca tatccggttc
     8881 cggaatcgca tcttcacctt tccgcgggag agtccgccca taacaaagaa ctctcctggc
     8941 tcgagttccg tgagttcggc gacctcttta cgggatgaga agaagagact gaccgctttt
     9001 agatcgtttt ctatcgagag tttcccgatg atctggttgt tgcactggga gaggacgttt
     9061 tttgtcacaa gagatggccg ctgggtcgca atcagcaagc cgagccctcg ttttcgcccc
     9121 cggcgggaga tctcctcgat ctgcttgatg gcgtctcctg attgcgggat gaatttgtcc
     9181 gcctcctcca ggatcagaag gaacggtttc ttcagtctgc tctccaggtt gtagagtacg
     9241 tcggcgagtt gggccacctt atcccgcata tcggtctccg agacgtcgaa gatcacggca
     9301 gtccttgaac agatcgcctc ctgcatgacg tcccggaggt ttgcccgctc gatatccacg
     9361 tcacgatcat cgccggaacc gatcctgatg agggggaacc gttccgcaag cgaggagtac
     9421 tccccctccg tatcgacgag gcagaacccg atcctcgcct gcaggagctg ttcgcagatc
     9481 accgcgatgc cccagctctt cccggcgcca gactgcgcta tgatgcacgt tcttcccgtg
     9541 acgagttcct gggcgtcgac ggcaacgtca tggtcaccgt cctttccgat caccagctcc
     9601 atccgctcaa agagataaca gggtgcccgg tcttttaacg cttcctcttc ggatcagagg
     9661 gtctggttca agcaaattag aggccttccc ccctattttt gttcctggag agcgagagaa
     9721 agaccccgaa gaccgagagg gccgtgaaca cctgaaagag cagccgcagg ctcttcatga
     9781 actccgggaa tactcccggg gtgacggtcg tcgaccccat gattacagca aagaccacca
     9841 ggacggcccc catgctcagc atcataccga gcgagcgcat cgtcgccacc atcgccgatg
     9901 cgctcccgta gaaccgtttc tccacgctcc ccatgatagc ggtggtgttc ggggaagaga
     9961 acaacccgac ccccgtcccc accaggaaca gtagggcgag gacgagtgtg atcggtgtcg
    10021 tctcgttgag cagcgagagt ccgaagagtc ccacggcagc aacccccatc ccaagcgagg
    10081 ccaggagccc tggctccaca cggtcggcca gtcgtcctgc cgccggggcg acgaagacct
    10141 ggacgatcgg ttgtatcagt aggagtgtgc ctgcggcggc gggttcgtat ccccggacat
    10201 actggaggta gagagagagg aggaacgcga ccgcatacgt tgcgctgtag ttgatcaggg
    10261 cggcaacgtt agaggctgca aagacacggt tcttctggag cagtgagatc ggtagaaggg
    10321 ggtctggccg gtgctgctcc acccggaaga agaccacgcc aagcaccagt gatgccgcaa
    10381 ggagcagcag accggcatgg gtggtgatcc aggccaggcc caggaagagg cagaggatca
    10441 gggctgatga gagggccagc cccgggagat cgaagtgttc catctctcgc tcgtttaaga
    10501 aagccgggaa cttcctggca tagaataaga ccgggactgc aaggagcgcg gttactatga
    10561 agatgcttcg ccacccaaag aactgggtca ggactccccc aaggaacggc ccgaacgaga
    10621 gcccggcgta gacagcggtg gtgttgatcc cgatcgcgta gccccgtctg ccaggcggaa
    10681 agaggctggc tatcagggcc acgcttgtcg cgttgatcat cgccccgccc accccctgca
    10741 cgaaccggaa gaggaggagg aggctcatcg aggatgtgaa tatggagagg atggatccgg
    10801 cggcgtagac gacgataccg atcaggaaga ttctgaccct cccccgcgag tcgccgagcc
    10861 ttcctcccgg gaggaggaat atgactgacg caaggagata ggtgctggag acccaggcca
    10921 gcgtggcagc atcggcggag aattcggtgc cgatgacagg cagggcaagg ttgatcgctg
    10981 aggctgtgaa aggatttata aacgaagcga tcgtgaccaa tacgaggatg accccccgcg
    11041 aataagttcg ttcttctacc tctttcacgc ctatcctcta cgcctccggg ttctgtgcgg
    11101 taacagggtt cgcattgagt gcagataagt ccactgccca tctccagcgg actactttta
    11161 ggatcatgac ccagtatatg ggcatgcgtg ccccacaccc ggagtcccag cgggatctcc
    11221 gtgcgacctc cctcaggctc atcctgctgc tgggcgtggc aagcctgttc ggtagcctgg
    11281 tctctaacgg tgccaggagc gttaccggcc cctacctcct ccttctcggt ggaagcgcgg
    11341 cagttgtggg gctggttgcc ggggccggcg agtttatcgg ctacgccctg cgggcggcca
    11401 ccggcgccta tcttggccat aaccaccgct actggaaggt ggcgattggc ggttactgta
    11461 tgctggcagc cattcccttc cttgcgttcg ccggtcactg ggcgacggcg gcagccctca
    11521 tcatcgccga gcggatcggg aaagccatca ggacaccggc gcgggacacg atcctttccc
    11581 atgcgacggt ggccgttggg agagggtggg ggttcgggtt tcacaaagcg ctcgatcagg
    11641 tcggtgcggt cgcgggcccg ctggtcatgg tcgccgccct ggccgtcacc gggggttata
    11701 cggatggatt cctggttctg gccatccctc tcatcggcgt cgcggttgct ctcttcatgg
    11761 cccggtcgga agtgccgtca ccacggttcc tggaggtgga ggagatgcag ggagacaaca
    11821 tcccttcgat cctgccctat gccttcttca tattcctggg aatggccggg tttgccacct
    11881 tcccgctcat atcgttccac cttaaggtcc aatcgatagt ctccgacgcc gcaatcccgc
    11941 tcttctatgc aggtgcgatg gttgtctctg tctttgtggc gctgttactc ggccgggtct
    12001 tcgatcgtgc tggtgtccat atctttcttt ccatccccat cataagcctt gcaactgtct
    12061 ttctcgcctt ctcgagcgat ccgcggatcg cggttgctgg atcgcttgtc tggggtgccg
    12121 ggattgggct ctttgaacca cttctacggg cgtcgattgc agagtccacg agccttgagc
    12181 ggagggggaa ggtctacggc acactgagtg cggtctacgg gacggcttgg tttctgggga
    12241 gcgccgtgat gggtgtcctc tacgacgtat cgatcgggca catcatcgcc tatgttgtta
    12301 tcatcgaggc cgcggccttg atagcctatc tctggttgtt actgatacag gatcccggtt
    12361 aggctcgatg aaaggccagg ggagatcgtc ccctgagcct tgcggcttta gtggtggagt
    12421 gccccgccga tggcgccacc gatcagggag aggatgccct ggtatatgaa cgccgccagg
    12481 actatgagga tcgacgctcc gagacccgcg atgaacccga acgccccgag gaggaccgtg
    12541 ccaccgataa gtataagcag cgatatgacg atggcgccaa gaagccctgc aagcagcccg
    12601 gcttttgcac cgttccagat cccgccccgg gcgatccagc cggcgacaaa tccgccggcc
    12661 agcggcccga ggacaggcaa caggaacccg agaagaaccc cgacaagcca gccgacgatg
    12721 actcctgtcc agaagttatc tgccatctac ttttactcac cgctgagtgt tcgagagagt
    12781 agtatataca tctttaggat caagtcaggt cgaatccccc ccatttcaat ggcgggatag
    12841 gtaacgtaac gaaagctctg taaaattgaa tcggccctgg atccaacctg gatttggaca
    12901 aaggagaaac agggccatgg atctcttagc gttcatcaaa ggttatcttt ccgatttgga
    12961 gaacggcatg aagaccctca tcacctggct cctcaaccag gtgatgctcc aggaggccct
    13021 ccagcaggcg ggagccaccc agtacgaacg caccgatgca cggaaggcgc atctgaacgg
    13081 ctacaaggat cggtctctca agacccgaag ttccgggaat tcccgttcga gatgcaggtc
    13141 ttcgggcgct atgcccgggt ggagaaggct caagtgaacg caatcgtcaa atcctgccac
    13201 cagggggttt caacgagaaa gatccaggag atcgccagtc ctccagggat cgaccaggtt
    13261 tcttcagtcc cggtttcccg aatagccagg gacctcgacg gccaggtgca ggcattcctc
    13321 ctgcggccga tcgaacaggc tatcccatac ctcttcgtgg atgcttcata ctacaaggtg
    13381 tgcaatggcg cacgctgcgc ccctctgatg atcctgacga acgtctgatt ggaagtgttg
    13441 cggtgacgta tgtggacgtg acgtgctcat cgagtcgtcc ggcatagacc ttgagacgag
    13501 gaaacgaccg catctcgagt acgaaggtgc gtactactat accctcgtct cgattcccgg
    13561 acacggtcaa aaaggtgtaa tcggaatgaa tgaaggacca gattctggga gccttctcaa
    13621 aacggtgggg attggcgccg tagcatccgg gatgaaaggg ataaaggagg tcacatgaaa
    13681 tcgaaacccg atagacgttt gatcatcgta ggggctcttg cgttcatcgg cgtcatcctc
    13741 ctcgccgcga cggttgtgct cacgt
//
LOCUS       DAOSZX010000032        13470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3859_length_13470_cov_21.850764, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000032 DAOSZX010000000
VERSION     DAOSZX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_13470_cov_21.850764"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..965
                     /locus_tag="PK196_04195"
     CDS             108..965
                     /locus_tag="PK196_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HON40653.1"
                     /translation="MRFYPGCTECLISRVEYESRLCIDDPARVAEIVGACRDLLQRIA
                     AEPLPAPVIASRVHRLAYRLIGNDDPYRLLKRENNMEAMAVCRGVRDDLATFRDLALA
                     SVIANTLDYGSVAHTVTENFVEFFREEFAAGLTVDETEAMEGLTSRVVYLADNCGEIV
                     FDALFADRLKKGGSHVTFAVRGAPILNDATLEDALALGLDRRVDLLTPTTDGIAELGL
                     NRDLVPPALADALDRATLVIAKGMANYESLSDDRDLPPVAYLMSVKCGPIGADIGIPV
                     GSRVALLRD"
     gene            complement(1121..2287)
                     /locus_tag="PK196_04200"
     CDS             complement(1121..2287)
                     /locus_tag="PK196_04200"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HON40654.1"
                     /translation="MLRVDFNSPIDPSTNQILDDKRFREHLPTVQALEDTRLVVLTHQ
                     SRPGKKDFTTLEAHAAKLERLLGRPVTYVDDIFGRCARETIRGAKRGDVLMLENLRFA
                     AEENLTLKPEEAKKTLIVRKLASMAEFYVNDAFGTAHRSQPSIVGLPLALPSVAGLLM
                     EKEITNLSRVFSDAPRPVTFVLGGTKVDDSIAVAQNVLAHGTADRVIVVGVVGNVFLL
                     AAGHRIGRSSERLIEDLGYRAEIERAKKLLEVHGDRIVLPDSVAVREEGERVDYPVNA
                     VPEDAQVLDIGTEAIRRLAEVITTSGTVVVNGPAGLFEEEPFALGTYEILRAASSVEF
                     SVVGGGHSGAAVERLGIEDRFTHISTGGGAAIEFLTGKKLPAIEALEMSRNLFW"
     gene            complement(2358..2984)
                     /locus_tag="PK196_04205"
     CDS             complement(2358..2984)
                     /locus_tag="PK196_04205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HON40655.1"
                     /translation="MALRDIKPTRSELISIKRRITLSERGYNILKMKRDGLILEFFKV
                     LQQAKDSRGVVAERYTRAMEMIALAETVEGAIRVKAAAFSVADVPQISLKSKNIMGVV
                     VPEIEASSVRKSVLDRGYGVLGTSAVIDETAEAFEDLLEAIIEAAEIETTMKRLLDEI
                     ESTKRRVNALEFKVIPELSEARDFIKMRLDEMEREELFRLKKIKARSA"
     gene            complement(2991..4376)
                     /locus_tag="PK196_04210"
     CDS             complement(2991..4376)
                     /locus_tag="PK196_04210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B"
                     /protein_id="HON40656.1"
                     /translation="MKEYRTITQIAGPLVFVEKTEPVGYGELVNIITADGTVKRGQVL
                     DTSDEIVVVQVFEATAGIGRDSGIRFTGETIKMPVGRDMLGRILSGGGKPIDGGPEIV
                     PEKRLDITGAAINPYARSSPEDFIQTGISTIDGMNTLVRGQKLPIFSASGLPHNDVAL
                     QIARQAKVPGSKEEFAVVFAAMGITREEANYFMADFEKTGALERSVVFLNLADDPAVE
                     RTITPRLALTTAEYLAFDLGYHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLASIYERAGIIKGVKGSVTQIPILTMPGDDITHPIPDLTGYITEGQIVVSRDLHRK
                     GIYPPINVLPSLSRLMNLGIGEGHTREDHKKVSDQLYAAYAEGNDLRGLVAIVGKDAL
                     SERDRMFLEFADLFEDRFVRQGLYEDRSIEETLDLGWELLSTLPEEHLVRIDRAMIQK
                     YHPKYRKKAEG"
     gene            complement(4379..>5023)
                     /locus_tag="PK196_04215"
     CDS             complement(4379..>5023)
                     /locus_tag="PK196_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HON40657.1"
                     /translation="AGRVINLNGTDGSVSVIGAVSPPGGDFSEPVTQNTLRIVKVFWA
                     LDAKLSQRRHFPAINWLNSYSLYLDRLNEWYDREVSPEWNPLRTWAMGILQKEAELQE
                     IVQLVGSDALPDEEQVTIEVARMIREVFLQQNAYDPVDTFCPMVKQYDMMKAIKRYAD
                     LARSAQAAGATPQQIISIKSKNELPQIKFIKDYKPELEKILKYMDAEFEGLRAV"
     assembly_gap    5024..5123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5124..5995)
                     /locus_tag="PK196_04220"
     CDS             complement(<5124..5995)
                     /locus_tag="PK196_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HON40658.1"
                     /translation="MKKRKENRTRGVLKRISGPVVTAVGLDAHMYDVVKVGKEELMGE
                     VIKIQGENVIIQVYEDTAGIRPGEPVENTGLSLSVELGPGLLTSIYDGIQRPLEVLVN
                     TMGNFIERGVSAPGLSHEKKWEFVPTVKKGDEVRGGDIIGTVQETNIVHKVMVPPTMK
                     GGRIREIRAGKFTVDETVCILEDGSEIRMLQRWPVRVPRPVKEKLNPDVPLITGQRIL
                     DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTEFP
                     ELEDPKTGKPLMERT"
     gene            complement(5995..6297)
                     /locus_tag="PK196_04225"
     CDS             complement(5995..6297)
                     /locus_tag="PK196_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HON40659.1"
                     /translation="MEIAVVGSEGFILGFRLAGVRKTYAAETNARLVEQINQVLDDRD
                     VGILVLKGSDMERIPLRLRTTLENSVWPTVIAIGEEEGGLSMRDRIKRSVGVDLWK"
     gene            complement(6299..7357)
                     /locus_tag="PK196_04230"
     CDS             complement(6299..7357)
                     /locus_tag="PK196_04230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HON40660.1"
                     /translation="MAGVSSGFATNYVYACTRMRVRRSRLIPRDDYLRMLNMSLPEII
                     RFIGETNYRSEIDELGTSFSGINLVEEALSWNLAKEFQKILELVPGELNYFTVSYLRR
                     WDIQNVITILRGKMQGVYPGKIKEVLVPAGKLDKVALDRLLTEESPERVVEALKGELF
                     YPTLEKEIRSAMETRSFAHLENELYKGYYARLIEDAKGGVKGGNVFLKYILLEIDIRN
                     IQTLFRLRAGHVQQDVRDMMIPGGTFSVDELQQILEVEDRDEFIDALKKRVKMVPLLN
                     TLEDIRRMSALHEIEVALTRVQLDQMERISKRYPFSVLPVIVYLEEKKYEVANLRALA
                     RGKEAGIPGERLKGYLVM"
     gene            complement(7372..7950)
                     /locus_tag="PK196_04235"
     CDS             complement(7372..7950)
                     /locus_tag="PK196_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HON40661.1"
                     /translation="MGLEAVVNEIREKGQREAEAIRAETRAEVEKILKEAQERAAAIK
                     SAVQEEVERAASHIINQETSAANLAVKRLILNAQKDVLDQVYSAALAAVGDLPAEFQE
                     KALTSLLKRAAKEIKEGVVHANERDTPIVMEILSRSKNLSGYTLGSPVEIPGGIIVES
                     KDGDLQIDYSYRTFLDEIWESCLKDVSDILFT"
     gene            complement(7962..8219)
                     /locus_tag="PK196_04240"
     CDS             complement(7962..8219)
                     /locus_tag="PK196_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HON40662.1"
                     /translation="MVDFGTAEVTLEMVQASQIGMKAFSAALAVGVTGIASAIAEMSI
                     GSAAVGATAENRDVFGLVLLLTVIPETIVIFGLVVALLLLF"
     gene            complement(8224..10185)
                     /locus_tag="PK196_04245"
     CDS             complement(8224..10185)
                     /locus_tag="PK196_04245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HON40663.1"
                     /translation="MLRPEGMRRLLIAAPKSEIETIIHELYRHNVYHIEDFVPESESE
                     GLSIGHPLPEASDAAEMLIKVRAIESACGIDPGSMEVKRKIPASKVREMIRTDLPAIQ
                     KEVEDLVNSRSKLEVQKKEHEQRARELEPFTAIPLDLEFYRGYRSFTVFAGHISRDIE
                     IGAPHEKYFSDKVTGNMIVVVVHNEHREEVERILLNARFQALTVPEENGAPADRLQAH
                     LEEVRRIDDEIAAINRRLAEIREQYAELLVACDELLTADVERAEAPLRFATTEETFIS
                     EGWVPDDRADEIQKALIRATGGRIYIEELEIEDDARVPVEYDNPPFAAPAQLLVDLYG
                     RPRYSELDPTLLVAIVFPIFFGLILGDVGYGAILLALSLGLRRFLKGEGMRSLLKVLT
                     YASISSIVFGILYSEFLGYSLPWEPLIFSRHLNIGGDATGHGPQVVELLILAIWIGIL
                     HVTLGRVLGMANARRLHHGKHATKAVIANAGWLGVMWGILLLIWSLFPIPLMPNLTGL
                     PVVLMGLNASAVAGAILLVAGIIGIAQENPLEIVELPTIFSHVLSYARLVAVGLSSVA
                     IAMMVNYIAIGMFIEPQLEAITPVGVIAIIFGILVFLLGHLLNTALGILGGGLHSLRL
                     HYVEFFTKFYKGGGIKYNPFGMRSKFMEE"
     gene            complement(10172..10501)
                     /locus_tag="PK196_04250"
     CDS             complement(10172..10501)
                     /locus_tag="PK196_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HON40664.1"
                     /translation="MKTDVLKSIKETEEEYQAMIHDAQAERRKSLSDAELEAENLISK
                     AQKDAEDYRNQRLAEARAEAQRRYAEIVREGEARAEALKASGRKNLTKAVDFVVTRFK
                     EQVHAET"
     gene            complement(10553..11032)
                     /locus_tag="PK196_04255"
     CDS             complement(10553..11032)
                     /locus_tag="PK196_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HON40665.1"
                     /translation="MIVPLDGETLLLRGEHSLLLAAKASSRFVLFIETVEGEYCQAVD
                     PDDLVAVSMPEGGPAEQAEMMLELVRRYHTPLVVLPKDHPGSGRLSMVVSVGPQIHLS
                     CTIQRGTHPEQHLLCSSEEFSGVSLSGIPGGVVIKNLPSRIEVVVLDAEKSAEDKQQ"
     gene            complement(11076..11228)
                     /locus_tag="PK196_04260"
     CDS             complement(11076..11228)
                     /locus_tag="PK196_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40666.1"
                     /translation="MCTVGGPVDIEFNERVKGKNYRCKDCGERFKGLGKRPMCPSCQS
                     ENVEEV"
     gene            complement(11295..13175)
                     /locus_tag="PK196_04265"
     CDS             complement(11295..13175)
                     /locus_tag="PK196_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HON40667.1"
                     /translation="MRYRDLRKDLVAANIGVTMEHYLLLAFLVSLLIGAACATAAFLA
                     MQLLPLPQFSLGIYNVFCIGLPDYVLTDRVFTALHLLVSVVIFILAAYASRALALRYP
                     AIHKTTRATRINLLLHNAVSYMYAMRQGGAEMVDIFRSLSENAGIYGEVADEFRRVVR
                     DTDYFGCDLITALQNLQETTPSEKLQDFIQDLISVIESGGDVLEYLETRVRLYQEDAK
                     FEQQNFLSTLQMAAESYVALFVAGPLFLIIVMIVMGFLSSTPIMQLSVVIYLLIPVGS
                     LFFILFIDIISIKTETVERYTSAKWLHEFDDVRVKKRAGEESLFRKLRNYDRIRDLRS
                     FLRHPLQAFLIDPNRTFYVTIPIAVVYVILLLTVTPAYPDIEVLIDVLDDHLIVALLI
                     MLVPFGICYQVWRTKVMRIEASIPEFLSRLSGINQVGLTLAQAISILASADLGVLTYE
                     IRKIQRDIEWGASVQEALIRFEVRIRTPAIARAVTLITTASRMTGSIGEVLNIAARDA
                     AISENLKRERSAEMSIYTVIVYLVFIVFLFVVAVIDSQFLTVLAGVETVSAAGAGMGA
                     IPLGTTPIATFERLLFHGVLIQAIFSGLIAGQMGEASVRAGVKHAAVMLIIALLVFAV
                     IL"
     gene            complement(13235..>13470)
                     /locus_tag="PK196_04270"
     CDS             complement(13235..>13470)
                     /locus_tag="PK196_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HON40668.1"
                     /translation="DTFSYTGRSRIFAEIMEYRGWGLKRLEEELQTRRTILRAMLDQG
                     IRDYRSVARIIQAWTISPETVLSSIDDLGELIR"
BASE COUNT         2665 a         4032 c         3718 g         2955 t
ORIGIN      
        1 ggtagcctgg ccatcctagg agactggggg tcttctgacc tgcgttcaaa tcgcagtggc
       61 cccattccac tactattttc agtctgtaca accagatagt cttcagcatg aggttctatc
      121 cgggctgcac ggagtgtctg atcagtcggg tcgagtatga gagccggctc tgcatcgacg
      181 atccggcgcg tgtcgctgag attgtcggtg cctgccgtga tctccttcag cgcatcgctg
      241 cggagccgct acccgcccct gtcatcgcga gccgggtgca caggctcgca taccgtttga
      301 tcggcaacga cgacccatat cggttgctga agagggagaa caatatggag gcgatggcgg
      361 tctgcagagg ggtgcgcgac gaccttgcga ctttccgcga tctggcgctt gcgtcggtca
      421 tcgccaacac cctggactac ggctcggtgg cgcacaccgt caccgagaac tttgttgagt
      481 tcttccggga ggagtttgcg gcagggttga ccgttgatga gaccgaggct atggaggggc
      541 tcacctcccg tgtggtttac ctcgctgaca actgcggaga gatcgtattt gacgccctct
      601 ttgctgatcg cctcaagaag ggcgggtcgc atgtcacatt tgccgtccgc ggtgcaccga
      661 tcttaaacga cgcgaccctt gaggatgccc tggcgctcgg gctcgatcgc cgggtcgacc
      721 tgctcacccc gacaacagac gggatcgccg agcttggtct caatcgcgac cttgtcccgc
      781 cggcgcttgc cgatgctctc gatcgcgcaa cgctcgttat cgccaagggc atggccaact
      841 acgagtctct ctccgacgat cgcgaccttc cgccggtggc ttacctgatg tcggtcaagt
      901 gcggtccaat cggggctgat ataggcatcc cggtcggttc ccgggttgct ctcctccgtg
      961 attgaaccaa actgactggc cgatggaacg gtggggactg caaccacgaa tgaggaggga
     1021 tgagtcccgc tgtcaggctg gctggcatat tacctgtgag ccatgcgttc aacgtgccct
     1081 ggaaaataga tgatccggga cggcggactg atctgcttca tcaccagaag aggttccggg
     1141 acatctccag cgcctcaatg gcgggcagtt tcttccctgt caggaactcg atcgccgccc
     1201 cgccaccggt ggagatatgc gtgaaccggt cctcgatccc gagccgctcg acggccgcgc
     1261 cggagtgccc gccaccaaca accgagaact ccacgctgga tgcagccctc aggatctcgt
     1321 aagtgccgag ggcgaacggc tcctcctcaa ataacccggc cgggccgttc acaacaaccg
     1381 tccctgacgt cgttatcacc tcagcaagtc tccggatcgc ctctgttccg atatcgagga
     1441 cctgggcgtc ttcggggacg gcgttcaccg ggtagtcgac ccgctcccct tcttcgcgca
     1501 cggcgactga atcgggtaga acgatccggt ctccgtggac ctcaagaagt tttttagccc
     1561 tctcgatctc ggcccggtag cccaggtctt cgatcagccg ctcggatgac cgcccgatac
     1621 ggtgacctgc cgcaagcagg aagacgtttc cgaccacccc gaccacgatc accctgtcgg
     1681 ccgtcccgtg tgcaaggaca ttctgtgcga ccgcgattga gtcatccacc ttcgtcccgc
     1741 cgagcacaaa cgtcaccggc cttggagcgt ctgaaaagac acgtgagagg tttgtgatct
     1801 ctttctccat caggagcccg gccaccgatg ggagcgcaag cggcagcccc acgatcgacg
     1861 gctgcgagcg gtgagccgtc ccgaaagcgt cgttgacgta gaactcggcc atagacgcca
     1921 gtttcctgac aatgagtgtc ttctttgcct cctccggctt cagggtcagg ttctcctcgg
     1981 cggcgaaccg caggttctca agcatcagga cgtccccacg tttcgcaccc ctgatggttt
     2041 cacgtgcgca ccgcccaaag atgtcatcga cataggtcac ggggcggccg agcagtcgct
     2101 ccagttttgc cgcgtgcgcc tcaagtgtcg tgaaatcctt tttgccgggt ctgctctgat
     2161 gcgtcaggac aaccagtctt gtatcctcga gagcctgcac cgttggcagg tgttcccgaa
     2221 accgtttgtc gtccaggatc tggtttgtag agggatcgat gggtgagttg aagtcaaccc
     2281 tcagcatcac cgttcccgtc agctttccag catctgcaag ggtagcgatc tccaccgggc
     2341 atcaccagtc tcccagatca ggcgctccgt gccttaatct ttttcaggcg gaagagctcc
     2401 tcgcgctcca tctcgtcgag tcgcatcttg atgaaatctc tggcttctga aagttccggg
     2461 atcaccttga actcgagcgc gttcacccga cgtttggtgc tctcgatctc gtcgagcagt
     2521 cgtttcatcg tcgtctcgat ctccgctgcc tcgatgatcg cttcgagaag atcctcaaac
     2581 gcctctgccg tctcgtcgat gacggcggaa gtcccgagaa ccccgtagcc gcggtcaagc
     2641 acgctcttcc gaacggatga cgcctcgatc tcggggacaa ccacgcccat gatgttcttg
     2701 ctcttgagcg agatctgcgg aacgtccgcc accgagaagg cagcggcctt caccctgatc
     2761 gcaccctcga cggtctctgc gagggcgatc atctccatag cacgtgtata cctctcggcc
     2821 acgactcctc ggctgtcttt tgcctgctgc agcaccttga agaactccag gatcagcccg
     2881 tcgcgcttca tcttgaggat gttatagccg cgctccgaga gtgtgatccg ccgcttgatg
     2941 ctgatcagtt cagaacgggt cggcttgatg tctcgcagcg ccatggatgc ttacccctct
     3001 gccttcttcc ggtatttcgg gtggtacttc tggatcatcg cgcggtcgat acgcacgaga
     3061 tgctcttcgg gcagcgtcga aagcagctcc cagccgagat cgagagtctc ctcgatcgag
     3121 cggtcctcgt agagaccctg tctgacaaac cggtcctcaa agagatctgc gaactcaagg
     3181 aacatacgat cgcgctccga gagcgcgtct tttccgacga tggcaacaag cccacgcagg
     3241 tcgttacctt cagcatacgc ggcatacagc tggtcagaga ccttcttgtg gtcctcgcgt
     3301 gtgtgcccct cgccgatacc caggttcatc agacgggata gcgatggcag gacgttgatc
     3361 ggagggtaga tacccttcct gtgcaggtca cggctgacca cgatctgtcc ttcggtgatg
     3421 tagccggtca gatccgggat cgggtgggtg atatcgtcgc cgggcatcgt caggatcggg
     3481 atctgggtca ccgagccctt gacacccttg atgataccgg cacgctcgta gatgctcgca
     3541 aggtcggtgt acatgtagcc ggggtaaccg cgacggccgg ggacctcctc acgggctgcg
     3601 cctatctggc ggagcgcctc gcagtagttg gtcatatccg tcaggatgac aagcacgtgg
     3661 taaccgagat cgaacgcgag gtattcggcg gttgtgagcg caagacgcgg cgtgatcgtt
     3721 cgctcaacgg ccgggtcgtc ggcgaggttc aggaagacaa ccgaacgctc gagagcgccg
     3781 gtcttctcga aatcggccat gaagtagttg gcctcttcac gggttatgcc catggccgca
     3841 aagaccacgg caaactcttc cttcgatccg ggcaccttcg cctgccgggc gatctggagc
     3901 gccacatcgt tgtggggcag accggatgca gagaagatcg ggagtttctg gccacggacc
     3961 agtgtgttca tgccgtcgat ggtcgagata cccgtctgga tgaaatcctc gggcgacgag
     4021 cgggcgtagg ggttgatggc cgcgccggtg atatcgagcc tcttttcggg cacgatctcg
     4081 gggccgccgt cgatgggttt gcccccgccc gagaggatac gcccgagcat atccctgccg
     4141 accggcatct tgattgtctc gccggtaaag cggatgcccg aatccctgcc aatcccggcc
     4201 gtagcctcga agacctggac aacaacgatc tcgtcgcttg tatccagcac ctggccgcgc
     4261 ttgaccgtgc catcagcggt tatgatgttg acgagttcgc cgtagcccac aggttctgtc
     4321 ttctcgacaa agaccagcgg ccctgcaatc tgcgtaatcg ttctgtactc cttcattctc
     4381 acaccgccct caagccttcg aattcagcat ccatatactt caagatcttc tcaagttcag
     4441 gcttgtagtc ctttatgaac ttgatctggg gcagttcgtt cttgctcttt atgctgatga
     4501 tctgctgggg ggtcgcgcca gctgcctgtg cgctgcgagc aaggtcggcg tagcgcttga
     4561 tcgccttcat catgtcgtac tgcttgacca tcgggcagaa cgtatccacc gggtcgtagg
     4621 cgttctgctg caggaagacc tcgcggatca tcctggccac ctcgatcgtg acctgttcct
     4681 cgtccggcag cgcatctgaa ccaaccagct gtacaatctc ctggagttcg gcctctttct
     4741 ggaggatacc catagcccat gttctgagcg gattccattc cggtgagacc tcccggtcat
     4801 accactcatt gagtctgtcc aggtagagcg agtaggagtt cagccagttg atagccggga
     4861 agtgccggcg ctgggagagt ttcgcatcca gcgcccagaa caccttgact atacgcaggg
     4921 tgttctgggt gaccggttcg gagaaatccc caccaggcgg cgagacagct ccgatgacag
     4981 agacagagcc atccgtcccg ttcaagttga tgacgcggcc tgcnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnngtccgct ccatcagggg tttaccggtc tttgggtcct
     5161 caagttccgg gaactcggtc agaacctcgg tcatctcgtt gccgcgttcg ccgcaaccga
     5221 tgtagacgac gatctcggca tccgaccact ttgccagctg ctgctgcgtg accgtcttgc
     5281 cgctcccgaa tggacccggg atggccgcgg ttccgccctt tgcgatcggg aagagaccat
     5341 ccaggatccg ctgaccggtg atcagcggga cgtcagggtt cagtttctct ttcaccggac
     5401 ggggcacgcg aaccggccac cgctggagca tcctgatctc gctaccgtcc tccaggatac
     5461 agaccgtctc atcgaccgtg aattttccgg ccctgatctc cctgatcctg ccgcctttca
     5521 tggtgggcgg gaccatgacc ttgtggacga tgttcgtctc ctgaacggtg ccaatgatgt
     5581 caccgcctct gacctcatcg cctttcttca ccgtgggcac gaactcccat ttcttctcgt
     5641 gggagaggcc cggggccgag acaccgcgct cgataaagtt gcccatcgtg ttcacgagca
     5701 cctcaagcgg ccgctggatc ccgtcgtaga tactggtcag cagccccggt ccgagttcaa
     5761 ccgagagtga gagacccgta ttctccactg gctcaccggg cctgatgccg gcggtatcct
     5821 cgtagacctg gatgatgacg ttctcaccct ggatcttgat gacctccccc atcagttcct
     5881 ctttgccaac cttaaccacg tcgtacatgt gcgcatcgag accgacagcg gttacaaccg
     5941 gcccggaaat ccgcttcagg actccacgag tcctgttctc ttttcttttc ttcattactt
     6001 ccacagatca acacccaccg atctcttgat tctatctctc atcgaaaggc cgccctcttc
     6061 ctctccgatc gcgatcaccg tcggccagac ggagttctcg agggtggttc gaagccgcag
     6121 gggaattcgc tccatgtcgc tgcctttcag cacgaggatg ccgacgtccc tgtcatccag
     6181 cacctggttg atctgttcga ccagccgcgc gttggtctcg gccgcatagg tcttcctgac
     6241 acccgcaagc cgaaaaccga ggatgaatcc ctcactaccg acaactgcga tctccatact
     6301 acatcaccag gtagcccttc aaccgctcac cgggaatacc ggcctctttg ccccgggcga
     6361 gggcacgaag gtttgcgacc tcgtacttct tctcctcaag atagacgatg acgggcagga
     6421 cggagaacgg ataccgtttt gatatccgct ccatctggtc gagctggaca cgggtaagtg
     6481 caacctcgat ctcatggaga gcgctcattc tccggatatc ttcaagcgtg ttcaggagcg
     6541 gaaccatctt tacccgcttc tttaaggcgt cgataaactc gtccctgtcc tcaacctcca
     6601 ggatctgctg cagttcgtcc acactgaacg tgccgccagg gatcatcata tcccgaacat
     6661 cctgctggac atgccctgcc ctgagacgga aaagggtctg gatgttccgg atatcgatct
     6721 cgaggaggat gtacttgagg aatacgttcc cacccttcac gcctcccttt gcatcctcga
     6781 tgagtcgggc gtagtagcct ttgtagagct cgttctccag gtgagcaaac gatctggtct
     6841 ccattgcact gcggatctct ttctccaggg ttgggtagaa cagttccccc tttagcgcct
     6901 caacaacccg ttctggagac tcctcggtga gaagacgatc gagagcaacc ttgtccagtt
     6961 tgccggccgg gaccagaacc tctttgatct tgccggggta caccccctgc atcttgccgc
     7021 gcaggatggt gataacgttc tggatgtccc agcgacggag ataactcacg gtgaagtagt
     7081 ttagttcccc cggcacaagt tccaggatct tctggaactc cttggcgagg ttccagctca
     7141 aggcctcctc gaccaggttg atacctgaga aggaggttcc gagttcatcg atctcggacc
     7201 tgtagttggt ctcgccgatg aaccggatga tctcaggcag gctcatgttc agcatccgga
     7261 gatagtcatc gcgtggaatc aaccgcgatc tgcgcacccg catgcgggtg caggcgtaga
     7321 catagttggt tgcaaatccg ctacttaccc ctgccatata cggaccctcc ttcatgtgaa
     7381 cagtatatct gacacgtcct tcagacagga ttcccagatc tcatccagga acgtacggta
     7441 actgtagtcg atctgcagat cgccgtcttt gctctcgaca atgatgccgc cggggatctc
     7501 gaccgggcta cccagagtgt agcccgacaa gttcttgctt ctggagagga tctccattac
     7561 aatcggggta tcccgttcgt ttgcgtggac aacgccttct ttgatctcct ttgccgctct
     7621 cttcagcaga gatgtgagcg ccttctcctg gaactcggcc ggcagatcac cgacagcggc
     7681 aagcgcggcc gagtagacct gatcgaggac atccttctgg gcgtttaaga tcagccgctt
     7741 tactgctagg ttcgccgcgg aggtctcctg gttgatgatg tggctggccg cccgttccac
     7801 ttcttcctgc accgcgctct tgatcgcagc agcgcgctcc tgtgcctctt tcaggatctt
     7861 ctctacttca gcccgggtct ccgcccggat cgcctcggcc tccctttgcc ctttctcccg
     7921 gatctcgttc acgactgctt cgagtcccat ggtcaactcc atcagaacag aagcagcagt
     7981 gcaaccacaa gaccgaagat aacaattgtt tcggggataa ccgtcagcag cagcacaaga
     8041 ccgaatacgt cacgattctc cgccgttgca ccgacggctg cagagccgat cgacatttct
     8101 gcaatggcgg atgctatgcc ggtcacaccg actgcaagag ctgcgctgaa tgctttcatt
     8161 cctatctgcg atgcctggac catttcaagg gtcacttcag ctgtgccaaa atctaccatt
     8221 tccttattcc tccataaact ttgatctcat accgaaagga ttgtacttta tgcctccacc
     8281 cttgtagaac ttggtgaaga actcaacata gtggagacga agtgaatgca gaccgcctcc
     8341 caggatgcca agtgctgtgt tcaacaggtg acccagcagg aagacgagta tgccaaagat
     8401 gatagcaatc acacctaccg gggtgatcgc ttcaagttgg ggttctataa acattccaat
     8461 cgcgatgtag ttgaccatca tcgcgatcgc gactgacgat aagcccacag ccacaagacg
     8521 ggcgtaggag agtacatgac tgaagatcgt tggaagttcc acaatctcaa gcgggttctc
     8581 ctgcgcaatc ccgatgatgc ctgccacaag caggatggcg cccgcaactg cgctggcgtt
     8641 cagccccatg agtacaaccg ggagccccgt aaggttgggc atcagcggta tgggaaagag
     8701 cgaccagatc aggagcagga taccccacat caccccaagc cagccggcgt ttgcgatgac
     8761 cgcctttgtt gcgtgtttgc cgtggtgaag tctccgtgca ttggccatgc cgagaacacg
     8821 cccgagcgtg acgtgcagga tgcctatcca gatcgcaagg atcaggagtt cgaccacctg
     8881 ggggccgtga ccggttgcat ctcccccgat gtttaggtgt ctggagaaga tcagcggttc
     8941 ccacgggagc gagtatccca gaaactcgct gtagagtatg ccaaagacga tgcttgagat
     9001 gcttgcgtag gtgagcacct tgagcagact cctcatcccc tcacccttca ggaacctgcg
     9061 caggccaagg ctcagcgcga gcaggatcgc cccgtaaccc acgtcaccca ggatcagccc
     9121 gaagaagatc gggaatacga tggctaccag cagcgtggga tcaagttcag agtagcgcgg
     9181 ccgcccgtag agatccacga gcaactgcgc cggggcggca aacggggggt tgtcatactc
     9241 aaccggcacc cttgcgtcgt cctcgatctc cagttcctcg atgtagatcc tgccgccggt
     9301 tgccctgatc agtgctttct ggatctcgtc agcccggtcg tcgggcaccc atccttcact
     9361 tatgaatgtt tcctccgtgg tagcaaaccg caacggggct tccgcccgct ctacgtcagc
     9421 cgtgagtagt tcatcgcatg ctaccaggag ttcggcgtac tgctccctga tctccgcaag
     9481 tcttcggttg atcgcagcaa tctcgtcatc gatccgccgg acctcctcca ggtgagcctg
     9541 gaggcggtca gccggtgccc cgttctcctc cggcacggtg agcgcctgga acctggcgtt
     9601 gaggaggatc cgttcgacct cttcccgatg ttcattatga accacgacaa cgatcatgtt
     9661 gccggtaact ttgtcggaga agtacttctc atgaggagcg ccgatctcaa tgtcacgcga
     9721 gatgtgcccc gcaaaaacgg taaaactcct gtagccccgg tagaactcaa ggtccagggg
     9781 gatcgcagtg aacggttcaa gttctcgcgc gcgctgctca tgctccttct tttgtacctc
     9841 aagttttgag cgcgagttga cgagatcctc cacctctttc tggatggcgg gcagatccgt
     9901 ccggatcatc tccctgacct tcgatgcagg gatctttctt ttgacctcca tgctgccggg
     9961 gtctatcccg catgcactct cgattgccct gaccttgatg agcatctcag ccgcatcgct
    10021 tgcctccggg agcgggtggc cgatcgagag cccctcagat tcgctctccg gcacaaagtc
    10081 ctcgatgtga tagacgttgt ggcggtagag ttcgtgaatg atggtctcga tctcgctctt
    10141 tggggccgcg atgagcagac ggcgcatccc ttcaggtctc agcatgcacc tgctccttaa
    10201 accgtgtaac cacaaaatct acagctttag taaggttctt tcttccagat gctttcagcg
    10261 cttctgcgcg cgcttcaccc tccctgacaa tctccgcata tctcctctgc gcttcggccc
    10321 ttgcctcagc aaggcgctga ttcctgtaat cctcggcatc cttctgtgcc ttggagatca
    10381 ggttttcagc ttccagctca gcatctgaga ggctcttcct cctctcggcc tgcgcatcgt
    10441 ggatcatcgc ctgatactct tcttctgttt ccttgatgct tttcaggaca tcggtcttca
    10501 tcaaaccctc cttctcacgg cgtatgaata tttgatgaaa atgagcatat atttattgtt
    10561 gtttgtcttc tgcggatttt tctgcatcca gaaccaccac ctcaattctg gaaggaaggt
    10621 tttttattac aactccaccg ggaatcccgg aaagcgaaac acccgagaac tcttccgatg
    10681 agcagagaag gtgctgctcc gggtgcgtac cgcgctggat ggtgcatgag agatgaatct
    10741 gtgggccaac cgagacgacc atggagaggc gccctgatcc gggatggtct ttagggagga
    10801 caacaagcgg ggtatggtag cgcctgacga gttcgagcat catctctgcc tgctcggcag
    10861 ggcctccttc cggcattgag acagcgacaa ggtcatccgg gtcgaccgcc tggcagtact
    10921 cgccctccac cgtctcgata aagagcacaa acctggaaga ggccttcgcg gccagaagaa
    10981 gagagtgctc acctcgcaga aggagggtct cgccgtcgag gggcactatc atctcgtccc
    11041 acctggcgag caaaccccct gatggtctct cgttttcaga cttcttcgac attttcagac
    11101 tggcagctcg ggcacatggg ccgctttcca agacccttga accgctcgcc acaatccttg
    11161 cagcggtagt ttttgccctt cacccgttca ttgaactcga tatcgacagg tccaccgacc
    11221 gtacacatct ggcatcacca ggaagggaaa ggaagtcaaa gcataaaact ctatcgcgcg
    11281 ggattgcatc gatcctacag gatgaccgcg aagaccagaa gtgcgatgat gagcatcacg
    11341 gctgcgtgtt tgacgccggc ccttactgaa gcctcaccca tctgtccggc gatcagcccc
    11401 gagaagatgg cctggatgag caccccatgg aagagcagac gttcaaacgt tgcaatgggt
    11461 gtggtgccga gagggatcgc gcccatgccc gctccagccg cggagacagt ctcgacaccg
    11521 gccaggacgg tgaggaactg ggagtcgatg acggccacga caaaaaggaa gacgatgaag
    11581 accaggtaga ctatgactgt gtagattgac atctcggcgc ttctctcccg cttgaggttc
    11641 tccgagatcg ctgcgtctct ggctgcaatg ttgagcacct cgcctatact gccggtcatc
    11701 cggcttgcgg tggtgatcag ggtgaccgcc ctggcgatgg ccggggtgcg tatccgcacc
    11761 tcaaaccgga tcagcgcctc ctgaacgctc gctccccact cgatatcacg ctggatcttt
    11821 ctgatctcgt atgtcagcac gccgagatcg gcggatgcca ggatcgagat cgcctgcgcc
    11881 agcgtcagcc ccacctggtt gatgcctgag aggcggctca agaactccgg gatgctcgcc
    11941 tcgatcctca tcaccttcgt tcgccagacc tggtagcaga tgccgaaagg aacgagcatg
    12001 atcagcagtg ccacgatcag gtggtcgtcc aggacatcga tcaggacctc gatatcaggg
    12061 taagcgggcg tcaccgtgag taggagaatt acataaacta cagcaatcgg gatggtcaca
    12121 taaaacgtcc tgtttggatc gatgagaaac gcctgcagcg ggtgccggag gaatgatcgc
    12181 agatcacgga tcctgtcgta gttgcgcagt ttccggaaga gtgactcttc accggcgcgc
    12241 ttcttgacgc ggacgtcatc gaactcgtgg agccattttg ccgaggtata tcgttcgacg
    12301 gtctcggtct tgatcgagat gatgtctatg aacaggataa agaagagcga accgaccggg
    12361 atgaggaggt agataacgac cgagagctgc atgatgggtg tgctgctcag aaaccccatc
    12421 acgatcatga cgatgatcag aaagagcggc ccggcgacaa agagcgccac ataggattcg
    12481 gcagccatct gaagggtgga gaggaagttc tgctgctcga actttgcatc ctcctggtag
    12541 agccgaaccc gcgtctccag gtattcaagg acgtccccac cgctctcgat caccgagatc
    12601 aggtcctgga tgaaatcctg tagtttctct gacggtgttg tctcctgcag gttctggagt
    12661 gcggttatga ggtcgcagcc gaagtaatcc gtgtccctga ccacccggcg gaactcgtct
    12721 gcaacttcac catagatccc tgcgttctcg ctgagggagc ggaatatatc caccatctcc
    12781 gcgcccccct gccgcatagc atacatgtaa gagacagcgt tatggagcag caggttgatc
    12841 cgggttgccc gggttgtctt gtgaatggca gggtagcgca gggcaagtgc gcgggatgca
    12901 taggctgcga gaatgaagat gaccacgctg actagaaggt gcagtgccgt aaacacccga
    12961 tcggtcagga cgtaatccgg caggccgatg caaaatacgt tgtagatccc gaggctgaac
    13021 tgagggagcg ggaggagttg catcgcaagg aatgctgctg tggcacaggc ggcaccgatg
    13081 agcagggaga caaggaacgc cagcagaagg tagtgctcca tcgtcacccc gatgtttgcc
    13141 gcaaccaggt ctttacggag gtcacggtat cgcatggggt tgcgctccac cctccgacgg
    13201 atgaagcgac ggaggtgcac gcgcagagat ggcatcagcg gatcaactca ccaagatcgt
    13261 cgatggagga gagcaccgtt tcagggctga tcgtccaggc ctggatgatc cgtgcgactg
    13321 acctgtaatc gcggatgccc tggtcgagca tagcgcggag gatcgtccgt cgggtctgca
    13381 gttcttcctc gagccgtttc agcccccacc cgcggtactc catgatctcg gcaaatatcc
    13441 gggatcttcc tgtgtaggag aaggtgtcgg
//
LOCUS       DAOSZX010000033        13402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3882_length_13402_cov_21.717240, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000033 DAOSZX010000000
VERSION     DAOSZX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_13402_cov_21.717240"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK196_04275"
     CDS             <1..588
                     /locus_tag="PK196_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HON40669.1"
                     /translation="PASHQISTEAMDTAATILIAIGLAMDAFAVSISGGVGLQKGEHR
                     WPLVAGTVFGGFQAGMPVLGWLGGTAIAGFLGFYGPWIAFLLLVLIGGKMLLEAVRGD
                     ENVRFANLSAATILLLAVATSIDALAVGVSLAILDIPILLPAAIIGIVTFAFSAAGVV
                     LGRSIGRIAGRNAGVIGGVILIGIGLQILLEHLLI"
     gene            994..1449
                     /locus_tag="PK196_04280"
     CDS             994..1449
                     /locus_tag="PK196_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40670.1"
                     /translation="MTSRMHTPHTTCPGCHEEVFLDELVGGHCPLCGYSLDDDDGTCS
                     EYEETIERSDLGWMVFQYYVFKRFCGEGATPLQVMQILSRYEEACQCNPLEAEKMQFT
                     LEVPMRRLERLLPKRCERCGRIFFRGGRAVISGDLASPNYRRFHICSSC"
     gene            complement(1450..2883)
                     /gene="proS"
                     /locus_tag="PK196_04285"
     CDS             complement(1450..2883)
                     /gene="proS"
                     /locus_tag="PK196_04285"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HON40671.1"
                     /translation="MEEDTGAIPRKEDFSSWYNEILWRAEIMDVRYPVKGLYVWYPYG
                     FGIRKRAYAILREIMDRDHAETMFPLLIPKTEFMKEAEHIKGFEDEVYWVTHGGRTEL
                     DVPLALRPTSETAIYPMYSLWIRSHTDLPLKLYQIVNTFRYETKHTRPLIRLREITSF
                     KEAHTVHATREEAEAQVEAAIRLYREFYERLRVPVIVSRRPAWDRFPGADYTIAVDTI
                     MPDGKTLQIGTVHMLGDHFSRTYEITYEDVNGEQQYAYQTCYGISERSIAAVISVHGD
                     DKGLVLPPEVAPVEIVIVPIIVGKRRDEVISAARALELELRDAGYTVKVDDRDMRPGA
                     KYYHWEMRGVPLRIEIGPRDIDANTVVAVTRTGRKTALDRRGVVEGIHSILAQFGEEI
                     SEAARRAMADRITAAETLEETAEALKTGIAVVHWCGSRECAEKIEAVVDASIIGSEIR
                     SDLITVSDGPCIACGSNGASTLVARTY"
     gene            complement(2919..3485)
                     /locus_tag="PK196_04290"
     CDS             complement(2919..3485)
                     /locus_tag="PK196_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HON40672.1"
                     /translation="MSENSLTNETIFAAVLFAVSIAIGVGFVILNPEFGDEVLSLFNE
                     QVVGQILSDSLAVLAFKIFLNNLSTCLLLFLGGASLGLVTMLILSANGLLIGVVTELV
                     RSQQGALYVVAALVPHGIFEIPAVLISGGLGFLLARALIAEWHGAADAAAEARSLARL
                     FLRVVIPLLAAAAVVEAFITPAILSMIT"
     gene            complement(3478..4323)
                     /locus_tag="PK196_04295"
     CDS             complement(3478..4323)
                     /locus_tag="PK196_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HON40673.1"
                     /translation="MIREFLYKYYIDPIRYGEAYTLVDTLTYALILIAAVYLLYRGLR
                     RYGIAIDDELVLATLPYVVFGGLLRVVEDTGMITSDLRFLLITPLIFFVVALIAGVAL
                     FGGKIAENAGIVSRYSKVYAGVGIAGSFLSGAALVWFGLTETTIALGVLAAILTLAAV
                     SSLALWAFMAYALNWGYVSNILYKLLIFGHMLDASATSYGIDLHPVHYVEQHVVGGAL
                     IDATGTAFSMFLLKFAVIVPAVYVLELYRREGDTGFWHLILLAMIVVGMAPGIRDLTR
                     MVLYV"
     gene            4335..5417
                     /locus_tag="PK196_04300"
     CDS             4335..5417
                     /locus_tag="PK196_04300"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HON40674.1"
                     /translation="MSADRINLLRTKVFDKSKWMQLPRDVLIGHDVIEQVPAVCEDLA
                     LGDSVLIVSGGRTRDVAGRRVESLLSASYDVRTFIAIDGDPLETIRKAEAAAAEVAFV
                     IGVGGGRIIDTAKIASYNTDRHFISIPTAASHDGIASSRASVPTAEGNVSLAAEPPIA
                     VVADTAVIASAPHRLLASGCADIIANYTAILDWELSHRLRGEPISEYALTLSRMTAEI
                     LFQNADLIKPNSEESAWIVTKALVSSGVAMSIAGSSRPASGGEHKFSHALERLAPGKG
                     LHGEKCGIGAIITMYLHGGDWRGIRDSLRKIGAPTTPAEIGVDDETAVEALLAARTIR
                     PERFTILDMGLTRESARNLVKMLYQE"
     gene            5425..5877
                     /locus_tag="PK196_04305"
     CDS             5425..5877
                     /locus_tag="PK196_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HON40675.1"
                     /translation="MAEEKAKVTLIGVTLAKPGLDFVYEGILSPECNGCKVRKICHNL
                     QPQKKYRIAAVRPNTRHACPIHHEAVIAVDVVEGPVVALISADMAIVNSKISYEFTCP
                     RTGCRGYRLCHPDGIIDGGKYVVDEVLGNAPDVCERGWILKLVELRPV"
     gene            complement(5962..6834)
                     /locus_tag="PK196_04310"
     CDS             complement(5962..6834)
                     /locus_tag="PK196_04310"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HON40676.1"
                     /translation="MLKTHGVGALIGLAIGDAMGAPLEGLHPPLMTVTEMEGGGIHDV
                     LPGQYTDDTLQAAALAQTLLACGGFDPDDFVRRLVLVYHTNPDFFGPTSRAVLDLIEG
                     GIPAVTAARLVHEERGGSRSNGCVMRGIPIGIFYPPDEVREVSLAASRVTHFDPAAGE
                     ASAFVNRMVSGMCRGEEAATSFGRALRACRDHELLGMLEDYRAYPPVPSLDAIVCTHC
                     AVRVFMGARSFKDAVIAAINLGGDADTVGAITGGLAGARFGFEAIPSSWLARLRDRDE
                     LLSLAWRLAGVSRP"
     gene            6903..7175
                     /locus_tag="PK196_04315"
     CDS             6903..7175
                     /locus_tag="PK196_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HON40677.1"
                     /translation="MQKEELLHLHMLLVQIKKYYETVTGEEIQADQYNALHISPVHIH
                     KNKVTHKKAILTLGNEIVQHIRANHNPYIQYQTDFQSQSERIATEH"
     gene            7179..8753
                     /locus_tag="PK196_04320"
     CDS             7179..8753
                     /locus_tag="PK196_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HON40678.1"
                     /translation="MSDTDTHREIISRILSTGHSPADVQKIKLEFCREYGIDMPRNSA
                     ILAAAAPDEVERLRPLLQVKPTRTLSGVAPVAVMTSPHPCPHGRCLPCPGGPEHPFGS
                     PQSYTGEEPAALRAREHGFDPYAQVQARLGQFEALGHHIEKAELIVMGGTITARPIDY
                     QEWFVGAAVQAMNDYPRVGTAPAGPDLDTIAASNESARVRCVAITFETRPDWCLREHI
                     NRMLAMGVTKVELGVQHLDDRILDYNRRGHSVAATVAANGLLRDAGLKVGFHVMPNLP
                     AASMDDDRWMFEELFADERFRPDFLKIYPTLVTPGTEIEALWERGEYQPYTEDELIDL
                     VAYAKSLLPEYVRLQRIQRDIPARLITAGSRHSNFRQLAGARLREQGGRCRCIRCREA
                     GRSPAPEEAAISTLAYRACGGEERFIQAGSKDTLIGFARLRFPQQPFREELTDAALLR
                     ELHVYGSLVPISAPASPEEWQHRSYGAQLLSRAEEEARDHGYRRLAVMSGVGVRPYYR
                     KQGYERVGPYMIKTLP"
     gene            8750..9865
                     /gene="priS"
                     /locus_tag="PK196_04325"
     CDS             8750..9865
                     /gene="priS"
                     /locus_tag="PK196_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HON40679.1"
                     /translation="MKPATLEFVKQRFADYYRRCNLIAPSSLEQREWGFVFFDAAPEL
                     RMRRHMAFADREELNAYVKNLVPAHVYYSTAYYQTPSAPKMSEKHWAGADLIFDLDAD
                     QIVRGPYAMMLARVKEETLKLIEMLTEELGFSRNRMRIVFSGGRGYHIHITDIAVLGW
                     GSQERREIVDYVCGIGLDPEMMLSSGGEPRGWRRRYTETLRGCLSHLAALDPAEATAR
                     LTGLEGIGKRTAAQFLADAGALSRAPAEELLQNRVVRAITASPEFEKRLRDAGAHADE
                     PVTTDIKRLIRTPGSLHGGSGMRVVPLDLHDLEDFDPLVDAVVFGERDIRVDVKANFV
                     ASMLGNTYTLKAGTQTVPEALAIYLCCRNTAEIAGGG"
     gene            9862..10530
                     /locus_tag="PK196_04330"
     CDS             9862..10530
                     /locus_tag="PK196_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40680.1"
                     /translation="MMVADILEKLRQMLLDMQHSGRLSHIEPRLYENARAYIEKLKED
                     YYSLENPLENREGGLIIEAIGSVNDTVQEIFSIRTRQILDLAFQQIEGEYVDKEETRK
                     MLPEERAMFKQIVEAIEECRKALIQGEVNLFSGEEVAAVEVTDEVGYEKIEPSAVPSP
                     PAPRTPGLTAYALVHVRSDVESFMGLDGRTYVLEEGDIITLPEKNAELLCEHNIGLNI
                     RLNK"
     gene            10568..10846
                     /locus_tag="PK196_04335"
     CDS             10568..10846
                     /locus_tag="PK196_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HON40681.1"
                     /translation="MKMPSKFKTYCPFCRSHQIHEVVKVKRGKVRHFNWIDRQKARRS
                     TVGNMGKFSKVPGGDKPTKRTNVKYRCTVCGKSHLRPGVRIGKFELTE"
     gene            10854..11042
                     /locus_tag="PK196_04340"
     CDS             10854..11042
                     /locus_tag="PK196_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HON40682.1"
                     /translation="MVRVSRENRSKFLKVKCPDCENEQLVFERASTIVECNICGRVLA
                     EPRGGKADIKAEVVAVVE"
     gene            11050..11829
                     /locus_tag="PK196_04345"
     CDS             11050..11829
                     /locus_tag="PK196_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HON40683.1"
                     /translation="MHEREWPEEGELVVCTVSDVKDFAAFVSLDEYNGRRGLIPISEI
                     ARGWIKYIRDYVREGQKVVCKVLNVDPDRGHIDLSLKDVNEHQRREKIHEWKNEQKAA
                     KWIGFAAEAAGVDRAIIEEAIYREYGQLYPAFEEIVSTGGEAAERLNLDEPVRRALIT
                     IANENVKVPRVTISGYLILSTPRPDGVNVIRRALRSAQPKIDNVDIDLVYIGAPKYRI
                     KVTAPDYKEAEKAIEKAANAAIGVVERGGGAGKFIRKQKTG"
     gene            11841..12008
                     /locus_tag="PK196_04350"
     CDS             11841..12008
                     /locus_tag="PK196_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HON40684.1"
                     /translation="MSSRIRRCPHDGRYTLSIICPACGQPCGPAHPARFSPQDRYGYY
                     RRTLRRWKTSQ"
     gene            11990..12727
                     /locus_tag="PK196_04355"
     CDS             11990..12727
                     /locus_tag="PK196_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HON40685.1"
                     /translation="MEDVTVTFTRDDEIEASVLIEGLPGIGHVGKLVAEHLIHELEAE
                     KIGEISSIYFPPQVVVDERGVAHLVNNEIYRCDKNGLAILFLVGDFQSTSAEGHYILT
                     ECYLDIAQDLGVRRIYTLGGYGVGHLVENPRVLAAVNMERLLSEVEGAGGAFEGGGMI
                     VGASGLLLGLGFARGIEGVCLMGETSGYIVDPRSADNLLTVLSCLLGIKVDHTLLQQR
                     AEEMERIIAKIQETEEARGREDLTYIG"
BASE COUNT         3046 a         3838 c         3952 g         2566 t
ORIGIN      
        1 ccagcatccc accagatctc tacggaagcg atggacacag ccgcaacaat actcatcgca
       61 atcgggcttg ccatggacgc gtttgccgtc tcgatcagcg gtggcgtggg gctgcagaag
      121 ggggagcatc gatggcccct agtcgctggc accgtctttg gcgggttcca ggcggggatg
      181 ccggtgcttg gctggcttgg cgggacagcg atcgcagggt tcctcggatt ctacggccca
      241 tggattgcgt tcctcctgct tgtgctgatc ggcggaaaga tgctcctcga ggcggtgcgc
      301 ggggatgaga acgtgcggtt tgcaaacttg agtgcagcca ctatcctgct gcttgccgtc
      361 gcgacaagca tcgatgcgct tgctgtcggt gtctcgctcg ccatactgga catccctatc
      421 ctcttgcccg cagccatcat cgggatcgtg acattcgcgt tctctgctgc cggagtggtt
      481 ctagggaggt ctattggccg gattgccggg cgaaacgctg gggtcatcgg cggtgtgatc
      541 cttatcggca tcggtctgca gattctgctc gaacaccttc tcatctgaga cagggtctac
      601 gcatgatact gccacaccaa tatgcataac cagcggtgtt caaaacaata actccagact
      661 gtgcccggat ttgatgaatc gcccgttcgt gttgcttgcg cggcttccca gggccaatcg
      721 ccacacactg ccctcccggg aagggggcgg ctggtcagcc gcaaaagacg ttcaccacag
      781 ggatgattcg aaggcatcaa aacatgagca aactggtttg atgggctcct ggtggtctca
      841 accgatcccg tataccccat tcgacacgta taagaacaca ccagacgcaa tacgtagtaa
      901 acctcatgaa aaaccgtata gaccgcgcat cccgcgtgga acgcttccga aagccgtatt
      961 cggaagagca aaactaatga ggcaagttca atcatgactt ccagaatgca taccccccac
     1021 accacctgtc caggctgcca tgaagaggta ttcctggacg aacttgtggg tgggcactgt
     1081 cccctctgcg ggtattccct cgatgacgac gatggaacat gcagtgaata cgaggagact
     1141 attgagcgct ccgatctcgg gtggatggtc ttccaatatt atgtcttcaa gcggttctgc
     1201 ggggagggtg caacacccct ccaggtcatg cagatcctct cccgctacga ggaggcctgc
     1261 cagtgcaacc ccctggaagc ggagaagatg cagttcactc tggaagtacc aatgcgtcgc
     1321 ctggagcgac tcctcccgaa gagatgtgaa aggtgcggga gaatattttt ccgcggtggt
     1381 agagcggtta tatcagggga ccttgcatcc cccaactacc ggaggttcca tatctgctcg
     1441 tcctgctgat cagtaggtcc gggcaaccag ggtagacgcg ccgttgctgc cgcaggcgat
     1501 acacggcccg tcggagacgg tgatgaggtc cgagcggatc tcggagccga tgatgcttgc
     1561 atccaccacc gcctcaatct tttctgcaca ctcgcgcgac ccgcaccagt gaaccactgc
     1621 aatcccggtc ttcaacgctt cagcggtctc ctcgagagtc tctgcagcgg tgatccgatc
     1681 ggccattgcc cgccgcgcag cctctgagat ctcctcccca aactgtgcaa ggatcgaatg
     1741 gatcccttca acaacaccac gccggtcgag tgcggtcttc ctgccggtgc gggtgacggc
     1801 gacaacggtg tttgcatcga tgtccctggg cccgatctcg atccgcaacg ggacgccgcg
     1861 catctcccag tggtaatact tcgcccccgg ccgcatatcc cggtcgtcaa ccttaacggt
     1921 gtagccagca tcccggagtt ccagttcaag cgccctggcc gccgagatga cctcatcgcg
     1981 cctcttcccg acgatgatcg gtacaattac tatctcgacc ggcgccacct ccggcggcag
     2041 caccagccct ttatcgtcgc cgtggacgct gatgacagcc gcaatagacc gttctgatat
     2101 gccgtagcag gtctgatagg catactgctg ctccccgtta acgtcctcgt aggttatctc
     2161 gtaggtgcgg gagaagtggt cgcccagcat atgaaccgtc ccgatctgga gcgtcttgcc
     2221 atcaggcatg atggtatcga cagcgatggt gtagtcggcg ccggggaacc tgtcccatgc
     2281 cgggcggcgg gagactatca ccggcaccct caaacgttca tagaactcac ggtagaggcg
     2341 aatcgcggcc tcaacctgcg cttccgcctc ctcccttgtt gcgtggacgg tgtgcgcctc
     2401 cttaaacgat gtgatctccc ggagacggat gagcgggcgg gtgtgctttg tctcgtagcg
     2461 aaacgtattg acgatctggt agagttttaa gggcagatcg gtgtgcgacc ggatccagag
     2521 cgagtacatc ggatagatgg cagtctcgct cgtaggacgg agggcgagcg ggacgtcgag
     2581 ttcggtcctg ccgccgtggg tgacccagta aacctcatcc tcaaacccct tgatatgttc
     2641 agcctccttc atgaactcgg tcttcgggat gagaagagga aacatcgtct cagcatggtc
     2701 gcggtccatg atctcccgga gaattgcgta tgcacgtttc ctgataccga aaccgtaggg
     2761 gtaccagaca tacagcccct tgacaggata acggacatcc atgatctcgg ctcgccagag
     2821 gatctcgttg taccagctcg aaaaatcttc tttccggggg attgcacctg tatcttcttc
     2881 catccgtctc ctacctcgaa actcttgatc tgccgctatt aagttatcat acttaggatt
     2941 gcgggcgtaa taaatgcctc cacaaccgca gcggccgcaa gaagagggat gacaacccgg
     3001 agaaagaggc gggcaagaga cctggcttct gcagcagcat ccgccgcacc atgccattca
     3061 gcgatgagcg cacgtgcaag gagaaaaccg agcccccccg atatcaggac ggcggggatc
     3121 tcgaagatgc cgtgcgggac gagcgccgcc accacgtaga gcgccccctg ctgagatctc
     3181 acaagttcgg tcaccacacc tatcaacagt ccgttggccg agaggatcag catcgtgacc
     3241 aggccaagag atgcgccccc caggaaaagg aggaggcagg tgctcaggtt gttcaagaag
     3301 atcttgaacg ccaggaccgc cagggaatca gagagtatct gacctaccac ctgctcattg
     3361 aatagcgata gaacctcatc cccgaactcc gggttaagga taacgaaacc gacgccgatg
     3421 gcgatcgata ctgcaaagag gacggccgca aagattgttt catttgtaag actgttctca
     3481 gacataaagc accatccgtg tgagatcgcg tatccccggc gccatcccga ctactatcat
     3541 ggcaagcagg atgaggtgcc agaaccccgt atccccctcg cggcggtaga gttccagaac
     3601 atacacggcg ggcacgatca cagcgaactt caggaggaac atagagaacg ccgtgcctgt
     3661 agcatcgata agcgcacccc ccacaacgtg ctgctcgacg taatggacag gatggagatc
     3721 tattccgtaa cttgttgcgc tcgcatccag catatgccca aagattaaga gtttgtagag
     3781 gatgtttgat acataacccc agttcagcgc gtatgccatg aacgcccaga gggcgagcga
     3841 cgagaccgct gcaagtgtca ggatggcggc gaggacgccg agcgcgatcg tcgtctcggt
     3901 gaggccaaac cagacaagcg ccgcgccaga gaggaaagag ccagcaatcc ccacaccagc
     3961 gtagaccttg ctgtagcggc tgacaatgcc ggcgttctcc gcgatcttcc ctccaaagag
     4021 ggcaacgcct gcgatcagag cgaccacgaa gaagatcagg ggcgtgatca ggagaaaccg
     4081 gagatcggag gtgatcatcc cggtgtcctc aacgaccctc aggagacccc caaagacaac
     4141 gtatggcagg gtcgcaagca ccagttcgtc atcgatggca atcccatagc gacgaagccc
     4201 ccggtaaaga agataaaccg ctgcaatgag gatcagggcg tatgtcaggg tatcgacaag
     4261 tgtatacgct tcaccatagc gtatcggatc gatgtaatat ttgtagagga actccctaat
     4321 cattgttgat ctaaatgagc gcagaccgca taaacttact ccgaacaaag gtctttgaca
     4381 agtccaaatg gatgcagctc ccgcgcgacg tcctcatcgg tcatgacgtc atcgagcagg
     4441 tcccggccgt ctgcgaagac cttgccctcg gcgactctgt gctcatcgta tctggggggc
     4501 ggacgcggga tgttgccgga aggcgggtcg agtctcttct ttcagcgtcc tatgatgtca
     4561 ggacgttcat cgcgatagat ggcgatcctc tcgagacgat cagaaaggcc gaggcagcgg
     4621 cggcagaggt cgcgtttgtg atcggcgttg gtggaggaag gatcattgac actgcaaaga
     4681 tcgcatccta caacaccgac cgccacttca tcagcatccc caccgctgcc tcgcacgacg
     4741 ggatagcctc gtcaagggca tcggttccta ccgccgaggg gaacgtctcg ctcgccgctg
     4801 agcctcccat cgccgtggtt gccgacaccg ccgtcatcgc atcagcacca caccggttgc
     4861 tcgcatccgg gtgtgcggac atcatcgcaa actataccgc gatcctcgac tgggaactct
     4921 ctcaccgtct ccggggcgaa cccatatcgg agtatgccct gacgctctcc cgcatgaccg
     4981 ccgagatcct ctttcagaac gcagacctca tcaagccgaa ctccgaggag agtgcctgga
     5041 tcgtaacgaa agccctggtt tcctcaggcg ttgccatgag catcgcaggc tcgtcccggc
     5101 ccgcaagcgg cggagagcac aagttctccc acgcgctgga gcgtctcgca ccaggaaagg
     5161 ggctccacgg tgagaagtgc ggtattggcg cgatcatcac gatgtacctc cacggcgggg
     5221 actggagagg gattcgtgac tcgctgcgaa agatcggcgc tccgaccacg ccggcggaga
     5281 tcggggtcga tgatgagacc gccgtggagg ccctccttgc ggcgcggacg atccggccgg
     5341 aacggttcac gatactggat atggggttga cgcgcgagtc tgcgcggaac ctggtgaaga
     5401 tgctctacca ggagtgagag tgtgatggca gaggagaagg caaaggtgac cctgatcggt
     5461 gtaacactcg caaaaccggg actcgacttc gtctacgaag gcattttgtc tccagagtgc
     5521 aatggctgca aggtgcgcaa gatctgtcac aaccttcagc cacaaaagaa gtatcggatc
     5581 gctgctgtcc gcccaaacac ccgccacgcc tgccccatcc accacgaggc ggtgatcgct
     5641 gtagacgtcg tggaagggcc ggttgtcgca ctgatcagcg ctgatatggc gatcgtgaac
     5701 tcaaaaatca gttatgagtt tacctgtccg cgaacgggct gccggggtta ccggctctgc
     5761 caccctgatg gcatcatcga cggagggaag tacgtcgtcg atgaggttct tggcaacgcc
     5821 cccgatgtat gcgagcgggg ctggattctc aaactcgtgg agctgcggcc ggtctgagag
     5881 actgctgctt cgggcccaat tttcccccaa cctggatttt tgtgctctgg tgggcccacg
     5941 gaggcttcac cgatggaatt cctatggcct gctgacccct gcaagccgcc aggcgagaga
     6001 gaggagttcg tcgcggtcgc gaagtctggc aagccacgat gacgggatgg cttcaaaccc
     6061 gaacctcgcc ccggcaagcc cgcctgtgat cgccccaacg gtgtctgcgt cacccccaag
     6121 gttgatagcc gcaatgaccg cgtctttaaa cgatctggca cccataaaga ccctgacagc
     6181 gcagtgggtg cagactatag catcaagcga cggcaccggc gggtatgccc gataatcctc
     6241 gagcatcccc aggagttcat gatcacggca ggctctgagc gcccggccga aggaagttgc
     6301 agcctcctca ccccggcaca tcccgctgac catcctgttg acgaacgccg aagcctcgcc
     6361 ggccgcggga tcaaagtggg tcacgcggga ggcggcaaga ctcacctccc gcacttcatc
     6421 cggtggataa aagatcccga tcgggatgcc gcgcatgacg cagccgttac tccggctgcc
     6481 cccccgctct tcgtggacca gtcttgcggc ggtcacggct ggaataccac cctcgatcag
     6541 gtcgaggacg gcacgcgatg tcggcccgaa gaaatccgga ttagtgtgat ataccaaaac
     6601 cagtcgccgg acgaagtcat cggggtcgaa acccccgcac gcaagaaggg tctgtgccag
     6661 ggccgctgcc tggagggtgt cgtcggtgta ctgccccggc aggacgtcat gaatcccgcc
     6721 gccctccatc tccgttacgg tcattagggg cggatggaga ccttcaaggg gcgcgcccat
     6781 ggcatccccg attgcaagcc cgataagggc tccgaccccg tgcgtcttga gcatataaaa
     6841 agatcaccaa caaaatatat ctacccgcgg acagtagtat tcttcgagga aaggtgataa
     6901 tcgtgcaaaa ggaagagctg ctccacctgc atatgctgtt agttcaaatc aagaagtact
     6961 acgagaccgt caccggggaa gagatccagg ccgaccagta taacgctctc cacatatccc
     7021 cggtacacat ccacaaaaac aaggtaacac acaagaaagc catcctgact ctcggaaacg
     7081 agattgtcca gcacatcagg gccaaccata acccctacat ccagtatcaa accgattttc
     7141 agtcacagtc cgaacgtatt gcaaccgaac actaaacgat gagcgatacc gatacacacc
     7201 gggagataat ctcccgcatc ctttctaccg ggcacagccc tgccgacgtc cagaagatca
     7261 agctggagtt ctgccgggag tacgggatcg atatgccccg aaactcggcg atccttgccg
     7321 ccgccgcacc cgatgaggtg gagcggctcc ggccgcttct ccaggtgaaa cccacacgga
     7381 cgctatcggg cgttgccccg gttgcggtga tgacctcacc ccacccctgc ccccacggca
     7441 ggtgtctccc atgccccgga ggacctgaac acccgtttgg gtcgccgcag agttatacag
     7501 gagaggagcc tgccgcactc cgggcaagag agcacgggtt cgacccctac gcccaggtgc
     7561 aggcacgtct cgggcagttt gaggcgctcg gccaccacat cgagaaggcg gagctgatcg
     7621 tgatgggcgg gactatcacc gcgcggccga tcgattacca ggagtggttt gtcggcgcag
     7681 ccgttcaggc gatgaacgat tatccgcggg tgggcacggc accagcagga cccgatctcg
     7741 atacaatcgc cgcctcaaac gagtctgcaa gggtccggtg tgttgcaata acctttgaga
     7801 cgcggccaga ctggtgcctc agagagcata ttaaccggat gcttgcgatg ggtgttacaa
     7861 aggtggaact tggcgtccag cacctggacg accgtatcct ggactacaac cgccgcgggc
     7921 atagcgtcgc ggccacagtc gcggcaaacg gcctcctgcg cgatgccggg ttgaaggtag
     7981 ggttccacgt gatgccgaac ctccccgcgg cgagcatgga cgacgatcgg tggatgtttg
     8041 aagaactctt tgccgatgaa cggttcagac cggatttcct gaagatatac ccgacactgg
     8101 tgacgccggg cacggagata gaggcgctct gggaacgcgg cgaataccag ccctacaccg
     8161 aggatgaact gatcgatctc gtcgcctatg caaagtctct cctcccggag tacgtccggc
     8221 tccagcgcat ccagcgggat atcccggcaa ggctgatcac cgcgggatcg aggcacagca
     8281 acttcaggca gcttgctggg gcgcgactcc gagagcaggg aggccggtgc cggtgcatcc
     8341 ggtgccgtga ggcagggcgg tcgcccgctc cggaagaggc ggctatctcg accctggcct
     8401 atcgtgcatg cggcggggag gagcggttca tacaggcagg ctcaaaagac accctcatcg
     8461 ggtttgcaag gctccggttt ccgcagcagc cgttccgcga agaactcacc gacgcagcac
     8521 tcctgcgcga actccatgtt tacgggagcc tggtcccgat aagcgcccct gcgtcccctg
     8581 aggagtggca gcaccgcagt tacggtgcgc agctcctctc aagagcggaa gaggaagcgc
     8641 gggatcacgg ttaccgccgt ctggcggtta tgagcggtgt gggtgtgcgg ccctactaca
     8701 ggaaacaggg atatgaacgt gtggggccat acatgatcaa gacgctccca tgaaacccgc
     8761 gacgctcgag ttcgtgaaac agaggtttgc cgactattac cggaggtgca acctcatcgc
     8821 cccctcctcc ctcgagcagc gcgaatgggg ttttgtcttc tttgacgccg caccagaact
     8881 ccggatgcgg cggcatatgg catttgccga ccgcgaggaa ctcaacgcct acgttaaaaa
     8941 cctggtcccc gcccacgtat actactcgac ggcctactac cagacaccgt cggcgccgaa
     9001 gatgagcgag aaacactggg ccggtgccga cctcatattc gatctcgatg ccgaccagat
     9061 agttcgtggc ccctatgcga tgatgcttgc gcgggtcaag gaggagacac tgaaactgat
     9121 cgagatgctg accgaagaac tggggttttc gcgcaaccgt atgcggatcg tcttctccgg
     9181 ggggcggggc tatcacatac acatcaccga tatcgccgtt ttaggctggg gaagccagga
     9241 gcggcgcgag attgtggatt acgtatgcgg gataggactc gatccagaga tgatgctctc
     9301 ttccgggggc gaaccgaggg gatggcggcg gcgctacacg gagacgcttc gaggctgcct
     9361 ctcacacctt gcagccctcg acccggcgga ggcaacggcc cgactcacgg gtcttgaggg
     9421 tatcggcaaa cggacggcag cacagttcct ggcagatgcc ggggccctct ccagggcccc
     9481 ggcagaggaa ctcctgcaga acagggttgt tcgggcaatc acggcatcac ccgagtttga
     9541 gaagaggctc agggacgccg gtgcgcatgc agatgaaccg gtcacgaccg atatcaaacg
     9601 gcttatccgg acaccgggat cgctgcacgg cggcagcggg atgcgggtcg tcccccttga
     9661 tctccacgac ctggaggact tcgatccact tgtggacgcg gtggtctttg gcgagaggga
     9721 tatccgggtg gacgttaagg caaacttcgt cgcatcaatg ctcgggaaca cctacacgct
     9781 caaggcaggg acccagacag tcccggaagc gcttgcgatc tatctatgct gcaggaacac
     9841 ggcagagata gcagggggtg gatgatggtc gccgatatcc tggagaaact ccggcagatg
     9901 ctgctggata tgcagcactc gggaaggctc tcacatatcg agccccgtct ctatgagaat
     9961 gcaagggctt acatcgagaa actcaaggag gattactaca gccttgagaa ccccctggaa
    10021 aaccgggaag ggggcctgat catcgaggcg atcggcagcg tcaacgacac cgtccaggag
    10081 atcttctcca tccggacaag acagatcctg gaccttgcgt tccagcagat cgagggggag
    10141 tacgtcgata aggaggagac ccgaaagatg ctccccgagg agcgggcgat gttcaaacag
    10201 atcgttgagg ccatcgaaga gtgccggaag gcgctcatcc agggtgaggt aaacctgttt
    10261 agcggggagg aagtggccgc cgtcgaggtg accgatgaag tgggttatga aaaaatagaa
    10321 ccctccgcgg taccgtcacc ccccgccccc agaacccccg ggttgacagc atacgccctt
    10381 gtacatgtgc gctccgatgt ggaatcgttc atgggactgg atgggagaac ctacgtgctt
    10441 gaagagggcg acattatcac cctccccgaa aagaatgcgg aactcctctg cgagcacaac
    10501 ataggcttaa atatcaggct taacaagtaa tataggtacc cgaatctatt gaatagaggt
    10561 taatatcatg aaaatgccgt ccaaattcaa gacatactgc ccgttctgca ggagtcacca
    10621 gattcacgag gttgtgaagg ttaagagggg caaagtgcgt cacttcaact ggatcgaccg
    10681 ccagaaggca cgcaggagca cggttggcaa catgggcaag ttcagcaagg ttcctggcgg
    10741 cgacaaaccg acaaagagga caaatgtcaa atacagatgc acggtctgtg ggaagtccca
    10801 tctccgcccc ggcgtcagga tcggcaaatt cgaacttacg gagtgataac agaatggtca
    10861 gggttagcag ggagaacagg agcaagttcc tgaaggtaaa gtgccccgac tgcgagaacg
    10921 agcaactcgt ctttgagcgg gcaagcacaa ttgttgagtg taacatctgc gggcgggttc
    10981 ttgcagaacc ccgtggcgga aaagccgata taaaggctga agttgttgct gtagttgagt
    11041 gatcgttcca tgcacgagag agagtggccc gaggaaggag aacttgtcgt ctgcaccgtc
    11101 tctgatgtca aggactttgc ggcgttcgtg agccttgatg agtacaacgg ccgtagaggg
    11161 ctcataccga tctccgagat agcgcggggc tggattaagt acatccggga ctacgtacgt
    11221 gaagggcaga aggtcgtctg caaggtcctg aacgtggatc ctgaccgtgg tcacatcgat
    11281 ctctcgttaa aagacgtgaa cgagcatcag cggcgtgaga agattcacga gtggaagaac
    11341 gagcagaaag ccgcaaaatg gatcgggttt gccgccgagg ctgccggcgt ggacagggcc
    11401 ataatcgaag aagccatata ccgtgaatat ggtcagttgt accccgcatt cgaagagatc
    11461 gtcagcacag gcggagaggc tgcagagagg ctgaacctgg atgaacctgt cagaagagca
    11521 ctcataacca tcgcaaacga gaacgtaaaa gttccaaggg tgacgatcag cggatatctg
    11581 atcctctcaa caccacggcc cgacggtgtc aacgtgatcc gccgggcgct acggagtgcg
    11641 cagccgaaga tagacaacgt ggatatcgat ctggtctaca tcggcgcccc gaaatacagg
    11701 ataaaggtga ccgcaccgga ttacaaggag gccgagaagg cgattgagaa ggcggcaaac
    11761 gccgccatcg gtgtggttga gcggggcggt ggcgccggga agtttatccg gaaacagaag
    11821 accggatagg tttttacaat atgagcagtc gtattcgccg atgtccacac gacgggagat
    11881 acactctctc aatcatctgc ccggcctgtg gtcagccatg cgggccggct catccggcac
    11941 gtttttcacc acaggatagg tacgggtatt acaggaggac gctgcgcaga tggaagacgt
    12001 cacagtaacc ttcacaagag atgatgagat cgaggcctca gtcctgatcg aggggctgcc
    12061 cggcataggg cacgttggga aacttgttgc cgagcacctg atccatgaac ttgaggccga
    12121 gaagatcggg gagatctcct cgatctattt cccgccgcag gtggtggtcg acgagcgtgg
    12181 cgtcgcacat cttgtcaaca atgagatcta tcgttgtgat aagaacgggc tggcgatact
    12241 ctttctcgtg ggggatttcc agagcacctc ggccgaaggc cattatatcc tgaccgaatg
    12301 ctatctggac attgcgcagg atcttggtgt gcggcggata tacaccttgg gaggctacgg
    12361 tgtcggccac ctggtcgaga acccgagggt gcttgccgcg gttaacatgg agcgcctcct
    12421 ctccgaggtg gagggcgccg gaggagcatt tgagggcggc gggatgatcg tcggggcttc
    12481 aggccttctg ctcggcctcg ggttcgcacg gggcatagag ggagtatgcc tgatgggcga
    12541 gacgagcggc tatatcgtcg atccaagaag cgccgacaac ctgctcacgg tgctctcctg
    12601 cctcctcggg atcaaggtcg accataccct tctgcaacag cgcgccgagg agatggaacg
    12661 gatcatagca aagatccagg agaccgaaga ggccagaggc cgtgaagacc tgacctatat
    12721 cgggtaattt tttcgcagat tcgcaaaata cagacagtct ttggcccttg agattgtcct
    12781 gcccatctcc ctgatgggca ggccgttcct gttaaccgca aggccgcatc acaggtcctt
    12841 ccggccaggc tacctctctc ctctgcccgg cgtactggtg aggcgatcgc ctcgcgcgca
    12901 ggagtctcgg caaactcctt ccgccggcga ggggtcattc tttctgtagt tctttcaggg
    12961 tcccggtaag agattttggg ctgaccccgc ccacgatctc catgatctcg ttgaaccgca
    13021 gccttttgtg gtggtcaggg atgctgatcg atcaaggagc gcccatctct tcgatatggg
    13081 ggtggtgttg ccttacaggg ggcatatcca atcgctactc tttttcatgc acaccggatt
    13141 actataaacc ttatatcatt aatattacac aaaagatata ctagcataca caatgaaatg
    13201 gaggtggatg aaaatgtgta acccggatca tcatcaccac ggtcacggct gctcttgcca
    13261 ggggccctgt ggatggatgc aatcgctcac catcgaggag gagatcgcta tgctggaagc
    13321 ccagaagacg cggatgcagg tcatgatcga tatgatagac cgacggatcg agagcctgaa
    13381 gaaaagggcc tgagaccatt tt
//
LOCUS       DAOSZX010000034        13205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3952_length_13205_cov_21.750875, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000034 DAOSZX010000000
VERSION     DAOSZX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3952_length_13205_cov_21.750875"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3103
                     /gene="nrdD"
                     /locus_tag="PK196_04360"
     CDS             <1..3103
                     /gene="nrdD"
                     /locus_tag="PK196_04360"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HON40686.1"
                     /translation="QGDLHIHDLEYFGTRPFCQDWDLRYFFYYGLMPDGNGTKASVAG
                     PAKRAEVAVLHAVKALGSAQTNFAGGQGYYNFLTFLAPYFEGMDYDGIKQLMQMFVYE
                     MTQMMVARGGQVVFSSVQLSPGVPTLWKDKPCVFRGKVWDGNQAPLRTYGEFEREVRL
                     LFKALMEVMLDGDYWGKPFSFPKPEISIEPDFLTEDEEFNRSHPDLPTYHDLYLMTFE
                     LASKYGTPYYDNQIPAYRGAGEGISCYQCLAGDELVPVADREGRISVKRIQDIFDTAA
                     KNGRRIDTFGTELAYYDGRTPSVDFETLEASLRPFHGVMRRRYSGPLLHITLESGRAI
                     TVTPDHPVYVLDGDVFVRDVAGELNVGDCLPVLKTTGFCERAVEEIEVAETLDTAGYG
                     DSIRVTWDGVTLRTSGRISLPRVLPASSELARLLGYYLAAGRSERSARRYTVRFTFEE
                     GDAEIAADTAACIRAVLGVEPQVLETASGTAVIISSRLIYLLFAALGCGSADADKSVP
                     DLVFNLDRSLVADYLGAVFNTTGKKDSGRPARSIRLNVASREVAQKLVWLSGRIGVQM
                     SYAERDRLFGLPPDSAPHRVYTCRITDNDQIERFQAATGYPPTMARGRENGCPFAQTP
                     ENAATPGCTGLACASRYGGDGVEKVQVTIVIPPLAGEEEEDLAGLLEYGDVHPLRIRS
                     IEPVDDECYVYDLVDVAGTHTFANALGVVTGNCCAYQFSSLADEDDEFEDKLYFREGK
                     HFSMGSWQVMSINCPRAAYKAEGDQERLFAELKSLMDTAVDLFRIKRRWMSLIRASGR
                     MPFAMQRPKDPNTGERGAVAVDLEGLVYTIGVVGVNEMVQHFTGYQLHESKEAFRLAV
                     RAMTELEIYARELSKKHNMTIALARTPAETTGQRFAVADLLDERFRDHAIRVVKGDAE
                     QALDMLGTTLDLPIYYTNGTHVTPGAAVPLTKRIEIEHVFFPIVDGGNIFHIWLGEAR
                     PDPRGLMEVAMNLCRTTQIGYFAFTRDLTVSLKEFHEYRPVRSGQTAERPVTITEVDR
                     ADA"
     gene            3198..4238
                     /locus_tag="PK196_04365"
     CDS             3198..4238
                     /locus_tag="PK196_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HON40687.1"
                     /translation="MIDLNNTLIAIDVGRGTQDILVYEPGRPIENSIKLVLPSPTVIV
                     AEKIREATRAGRPVFLEGFLMGGGANTRAVREHLSRGLPVYATPYAAATIHDNPERVQ
                     ELGVEIRNDPPSDAMTVRTTDYMEHELRQALALFGVGYPENVAVAVQDHGYSPHRSNR
                     INRFELMREHLDAGGWEILSLIADPPLPDMTRMQAVRRQAPEALVTDTGPVALIGALC
                     DPVVRRLARTGVTLVNAGNGHTLCFTLKGHEIHGLFEHHTGALNPEKLHHYIRRLADG
                     TLTTEEVFDDGGHGAAVRKPLAIDVVVATGPNRLRLLPEAYQAAPFGDMMLSGCFGLV
                     YLWKVFRERREG"
     gene            complement(4256..4705)
                     /locus_tag="PK196_04370"
     CDS             complement(4256..4705)
                     /locus_tag="PK196_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HON40688.1"
                     /translation="MPQVTLTEECMRLISQFESLTGAGSRDCVVDNRNERLIFVINPG
                     DMGLAIGKGGSSIKKASEVMGKRIEVVEYSTDPAQFIRNCFLPARVTDIEFDTDEESR
                     RIARIEVRDEDRGLAIGKAGKNIFKAKVLAQRQHDIDDVVLMQSETA"
     gene            complement(4709..4996)
                     /locus_tag="PK196_04375"
     CDS             complement(4709..4996)
                     /locus_tag="PK196_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HON40689.1"
                     /translation="MDFNASLRKAVKTGTVFLGRNRTRECIDEGKARLVVVAENSPES
                     VKNMVSGIDIPVYVYEGSSIQLGKACGMPYVVSALAVVEPGESDILNAVRV"
     gene            complement(5008..6180)
                     /gene="rpoA2"
                     /locus_tag="PK196_04380"
     CDS             complement(5008..6180)
                     /gene="rpoA2"
                     /locus_tag="PK196_04380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HON40690.1"
                     /translation="MNSEMEREIDALDLPYRTREDLKASLAGKEITSEQFKRIVEMVS
                     NEYQKSRIEPCEAVGVVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     REPSTPTMAVYLLDDWAFDRDRAREVSWQIEAAPLHEFGDITIDMENMQVLVLLNRAV
                     CDRRKIAVDEIIEIAPRKIRERRHYRDFEVEADPKKPSITFTPKNQESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKEGGEYILYTEGSNLKDVFEVKGVDTSRTRSNNISEIADV
                     LGIEAGRNAIIQEALTTLNEQGIGVDVRHIMLVADMMCMDGEVKQIGRHGIAGEKESV
                     LSRAAFEVTVNHLLDAAITNEVDELNGVTENVIVGQPIQLGTGDVKLIAKPLNLKT"
     gene            complement(6177..8825)
                     /locus_tag="PK196_04385"
     CDS             complement(6177..8825)
                     /locus_tag="PK196_04385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HON40691.1"
                     /translation="MTSPKRVGKIEFGLLSPKEIRKMSVRKIIWADTYDDDGFPYPQG
                     LMDLSLGVIDPGLRCKTCDQKAADCPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRSC
                     SRLLMTAEEVEKIVGTEDSDLTPEVVSEKDIKKERVCPHCGEQQLKINFEKPTTFSEV
                     FLEDGRKIEHKLTPADIRARLEKIPDEDLRALGINPDVARPEWTILTVLPVPPVTMRP
                     SIILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTTYLDN
                     EVAGCPPARHRSGRPLKTLSQRLKGKEGRFRGSLSGKRVNFSARTVISPDPNLSITEV
                     GIPLAIANEMTIPVRVTPFNIEEMRHYVLNGPVRPTIRSPCGANYVIRPDNRRIRLSE
                     VNLETVTEMLEPGWTVERQIKDGDIVLFNRQPSLHRMSIMAHRVVVMDGKTFRLNPAV
                     CPPYNADFDGDEMNLHIPQTEEARAEAEILIAVQANILSPRTGGPIIGGIHDHISGIF
                     LLTHKIRHFDRAMTLYLTRNLPIDHLPEPAAKDGDENPLWTNKQVFSLILPDDLNMIF
                     RASSCQNCETCRQERCENDAYVRIVDGNLLSGTIDKKAIGAFDGQILHRIIRQHGMKR
                     AARFIDDVTKLSIQAIMLEGFSFGIDDEDLSRTEYGQIEEVLNNAITDVERRIRIYNE
                     GQLEPMPGRTLEETLEMQIMQVLGKARDRTGEIAGQRLGMDNSAVVMAVSGARGSMLN
                     LTQMAGCLGQQSVRGERIMRGYEDRTLPHFRRGDRGAKAHGFITNSYKSGLSPTEFFF
                     HAIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRTTGGRIIQFRYGEDGT
                     DPAKSSYGAPVDVKGIVESVLREEVK"
     gene            complement(8847..10661)
                     /gene="rpoB"
                     /locus_tag="PK196_04390"
     CDS             complement(8847..10661)
                     /gene="rpoB"
                     /locus_tag="PK196_04390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HON40692.1"
                     /translation="MRQSRVFVDGALIGLVDNPRDLVSRLREMRRCGRISSEINISYK
                     EFNNDVIVHTDRGRARRPLIVVRDGLPAVTEEDIERLRNGGITFDDLVQKGAIEFVDA
                     EEEEDLLIAINEKDLTSEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGAANMKLRPDTRGHMLHYAQKPLIYTQTSDVIGSDDRPAGQNFVVAILSYEGFNI
                     EDALIINQASIDRGLGRSHFFRTYEGEERRYPGGQVDRIEIPDEEVSGAHGSEYYANL
                     DIDGVINPETVVREKDVLIGKTSPPRFLEEPTSELIAVEKRRDTSVTMRSNEHGIVDT
                     VIITEGENSSKLVKVRTRDLRIPEVGDKFASRHGQKGVIGLIQPAADMPFTESGMTPD
                     LIINPHAVPSRMTIGHMLEMLGGKVGSLDGRRIDATAFRGEREADLRKSLKALGFAHN
                     GREVMYDGVTGRQFAADIYIGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVTQWVCAKCGMVAMVDRKRKVTRCLT
                     CGGETDIYPVEMSYAFKLLLDEMKSMGIAPRLRLEDMV"
     gene            complement(10658..12199)
                     /locus_tag="PK196_04395"
     CDS             complement(10658..12199)
                     /locus_tag="PK196_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HON40693.1"
                     /translation="MSRAYFAREHVARHQLDSYNNFLLHNLQEVVDEQRVIETDIESR
                     GKGKEAVWVELGRIEVKKPLVREADGSQSELFPSEARLRNLTYAAPIQLEMTLVQGGE
                     PQDPIVTTIGQLPVMVGSAACNLYNLSDRDRIEHGEDPLDPGGYFIVNGTERVLMTLE
                     DLASNKIMTEFTERYNERIYVAKVFSQYRGYRALVIVERNRKNLLEVSFPSVAGHLRF
                     VDLMRALGLQGDHDIVEAVSTDEDILTFMMQNLEESECDTVDEGIMYVGKKLAPNQTR
                     DYQRKRAEFVLDNYLLPHLNHLMPAGLKEDDPGYRAAVESVRLAKAHFLGRMAEACFD
                     LVLERRRIDDKDHYANKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRHRDLSIG
                     TAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMAVLSHLRRVISPLSRSQPHF
                     EARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISNGVSNEEEVKNTLYEMGVIAL
                     RRETA"
     gene            complement(12243..12494)
                     /locus_tag="PK196_04400"
     CDS             complement(12243..12494)
                     /locus_tag="PK196_04400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HON40694.1"
                     /translation="MGTSLDVLKHEMVPDHQIMSEDEVAELLATYRISLEQLPKIYHD
                     DPAVKAIRGEVGNVIRIIRDSRTAGRAEAYRIVVKRPKK"
     gene            complement(12574..12647)
                     /locus_tag="PK196_04405"
     tRNA            complement(12574..12647)
                     /locus_tag="PK196_04405"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12611..12613),aa:Lys,seq:ttt)
     gene            12841..>13205
                     /locus_tag="PK196_04410"
     CDS             12841..>13205
                     /locus_tag="PK196_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HON40695.1"
                     /translation="MIPSRYYADNAVPAISWVTQGIYLLIAVALSVLALVSLYDVLLQ
                     MVALIHAEDMMQGVLEVLHAVLLTLILVEILETVTAYFRTKKVLVTPILIAGVTAMVR
                     RVLIFGVEVHEPAEIGMTLA"
BASE COUNT         2515 a         3930 c         3839 g         2921 t
ORIGIN      
        1 ccagggggat ctccacatcc acgacctgga gtattttggt acccggccgt tttgtcagga
       61 ctgggacctt cgctacttct tctactacgg tctgatgccc gatggcaacg ggacaaaagc
      121 ctctgttgcc ggcccggcaa agcgggctga ggtggcggtt ctccacgcgg tcaaggcgct
      181 cggctccgcc cagacaaact ttgccggcgg ccagggctac tacaacttcc tgacgttcct
      241 ggcaccatac tttgagggga tggactacga cgggatcaag cagttgatgc agatgttcgt
      301 ctacgagatg acccagatga tggtcgcccg gggcggtcag gtggtcttct cgtcggtcca
      361 gctctccccc ggcgttccca ccctctggaa ggacaagccc tgcgtcttcc gcggcaaggt
      421 ctgggacggg aaccaggcgc cgctccgaac ctacggcgag ttcgagcggg aggtcagact
      481 cctcttcaag gcgctgatgg aggtgatgct cgatggcgac tactggggta aaccgttctc
      541 cttcccgaaa cctgagatca gcatcgagcc cgatttcctc accgaggacg aggagttcaa
      601 ccgcagccac ccggacctcc ccacctacca cgacctctac ctgatgacgt ttgaacttgc
      661 atccaagtac ggcacccctt actacgacaa ccagataccg gcttaccggg gcgccggtga
      721 ggggatatcg tgttaccagt gcctggcggg ggacgaactg gttccggtgg ctgacagaga
      781 gggccggatc agtgtaaagc gcattcagga tatcttcgat accgcggcaa agaatgggag
      841 acggatagat actttcggca cagagctcgc ctactatgat ggaagaaccc cgagcgtcga
      901 tttcgaaacc ctggaagcgt cgctccgacc gttccacggt gttatgcgca gacggtatag
      961 cggccccctc ctccatataa cccttgagtc gggccgtgcg atcacggtca ccccggatca
     1021 tccggtctac gtactcgatg gagatgtgtt cgttcgggat gtggcaggcg aactcaacgt
     1081 gggtgactgt cttccggtgc tgaaaacgac cgggttctgc gagcgagcgg ttgaggagat
     1141 cgaggttgca gagacactgg atacggctgg atatggcgac tcgatccgcg tcacctggga
     1201 tggggtaacc ctcaggacat ccgggcgcat cagtctgccc cgcgttctgc cggccagcag
     1261 cgaacttgcc agactccttg gctactatct tgcagcaggg cgcagtgagc gttcagcgcg
     1321 gcgttatacg gtcaggttca cgtttgaaga aggcgatgca gagatcgcgg cggataccgc
     1381 cgcatgcatc cgtgcggtcc tgggggtcga gccgcaggtt ctggagacgg catcgggaac
     1441 ggcggtgatc atcagcagca ggctcatcta ccttctcttt gccgccctcg gctgtggttc
     1501 ggccgatgcc gataagtctg tgccggatct cgtcttcaac ctcgatcgtt ccctggtggc
     1561 ggattacctc ggtgcggtct tcaacacaac cgggaagaag gactccggcc gccccgccag
     1621 aagcatcagg ctgaatgtag cctccaggga ggttgcccag aaactggtct ggctgagcgg
     1681 caggatcgga gtccagatga gttacgccga gcgggatagg ctcttcgggc tgccgccaga
     1741 cagcgccccc cacagggtct acacctgcag gataacggac aacgaccaga tagagaggtt
     1801 ccaggcggcg accggttacc ctccaacgat ggcgcgggga cgggagaatg gctgtccgtt
     1861 cgcgcagacc cccgagaacg cggccacacc cggctgcacc ggtcttgcct gtgcaagcag
     1921 gtacggtggt gatggggtgg agaaggtcca ggtgaccatc gtcatcccgc ctcttgccgg
     1981 agaggaggaa gaagatcttg ccggtctcct tgagtatggg gatgtgcacc cgctccggat
     2041 tcgatcgatc gaaccggtgg acgacgaatg ttacgtctac gaccttgttg atgttgcggg
     2101 cacccacacc tttgcaaacg cgctcggggt cgtgaccggg aactgctgcg cctaccagtt
     2161 ctcctcgctt gctgacgaag acgacgagtt tgaggataag ctctacttcc gggaggggaa
     2221 acacttctcg atgggttcat ggcaggtgat gtcgatcaac tgcccccgtg cggcttacaa
     2281 ggccgagggt gaccaggaac gactctttgc cgagctcaag tctctcatgg acaccgccgt
     2341 cgacctcttc cgtatcaagc gccgatggat gtcactgatc cgggcgagcg gccggatgcc
     2401 gtttgcgatg cagcgcccga aggatccaaa caccggcgaa cgcggtgcgg ttgctgtgga
     2461 cctggaaggc ctcgtctaca ccataggtgt cgtcggcgtc aacgaaatgg tgcagcactt
     2521 caccggctac cagctccacg agtcgaagga agccttccgc ctggccgttc gtgcgatgac
     2581 cgaactagag atctacgccc gtgaactctc gaagaagcac aacatgacga tcgccctcgc
     2641 ccgcaccccc gcagagacga ccggccagcg gtttgcggtt gctgatctcc tggacgagcg
     2701 gttccgcgac catgcgatca gggtggtcaa gggcgacgcc gaacaagcgc tcgatatgct
     2761 tggcacgacg cttgaccttc ccatctacta caccaatgga acccatgtca cccccggagc
     2821 ggcagtcccg ctcacgaagc ggatcgagat cgagcacgtc ttcttcccca tagtggacgg
     2881 cggcaacatc ttccacatat ggctcgggga ggcgcgcccc gacccgcgtg ggctgatgga
     2941 ggtggcgatg aacctctgtc ggacgaccca gatcggttat tttgcattca ctcgcgacct
     3001 gacggtctcc ctcaaagagt tccacgaata caggccggtg agatccggac agacggctga
     3061 gcggccggtg acgatcacgg aggtcgaccg ggcggatgcc tgacgagggg tcataccttt
     3121 tttggatacg gggagacggt tccggcagag atgactcacc gtcctggaga taaagtaagt
     3181 ataatacgag gccataaatg atcgacctta ataacacgct catcgcgatc gacgtcggtc
     3241 ggggcaccca ggatatcctg gtctacgaac ccggccggcc gatcgagaac agcatcaaac
     3301 tggttcttcc ctctccaacc gtgatcgtgg cggagaagat ccgggaggca acccgggctg
     3361 gccgtccggt attcctggaa gggttcctga tgggcggcgg cgcaaacacc cgcgcggtgc
     3421 gggagcacct ctcccgcggt ctccccgtct atgccacacc gtatgcggca gcgaccatcc
     3481 acgacaaccc ggagcgtgtg caggagctgg gtgttgagat ccgaaatgat cccccctcag
     3541 atgcgatgac cgtccgcacc accgattaca tggagcatga gctcaggcag gctcttgccc
     3601 tctttggtgt gggttacccg gagaacgtcg cggtagccgt ccaggaccac ggttactcac
     3661 cacaccggag caaccgcata aaccggttcg aattgatgcg ggagcacctt gacgcggggg
     3721 gctgggagat cctctcgcta atcgccgatc ctccacttcc agatatgacc cggatgcagg
     3781 ccgtccggcg tcaggcgccg gaggcactcg tcacagatac cggccctgtc gccctcatcg
     3841 gggcgctctg cgaccccgtg gtcaggcgtc tggcccgcac cggggtgacc ctggtcaacg
     3901 ccggtaacgg ccacacgctt tgcttcaccc tgaaggggca tgagatccat gggctctttg
     3961 agcaccacac cggcgcactc aaccccgaga aactccacca ctacatccgg cggcttgccg
     4021 acggcaccct gaccacggag gaggtctttg atgatggcgg tcacggggcg gcggtcagga
     4081 aaccccttgc gatcgatgtc gtggtcgcca cagggccaaa ccgtctccgg ttgctgccgg
     4141 aagcttacca ggcggcgccg ttcggggata tgatgctatc cggctgcttc gggctcgtct
     4201 atctctggaa agtgtttcgg gagagaagag aggggtaacg cagaaaaagg ggtgttcagg
     4261 cggtctcact ctgcatcagc acaacatcgt cgatgtcatg ctggcgctgc gcgagtacct
     4321 ttgccttgaa gatgttcttc cccgccttgc cgatggcaag cccccggtcc tcgtcccgga
     4381 cctcgatgcg ggcgatccgc cggctctctt cgtccgtatc gaactcgata tcggtgaccc
     4441 tggcaggaag gaaacagttc cggatgaact gtgccgggtc ggtggaatac tccacaacct
     4501 cgatgcgctt ccccataacc tcggaagcct tcttgatgct ggatccaccc ttgccgatgg
     4561 caagtcccat atcccctgga ttgatcacga agatgagtcg ctcgttgcgg ttatcaacca
     4621 cgcaatcgcg gctgcctgcg ccggtgaggc tttcgaactg ggagatcagg cgcatacact
     4681 cctcagtcag cgtgacctgc ggcatcatct acactctcac ggcgttcagg atatcggact
     4741 cgcccggctc gaccacggca agggcgctga cgacataggg catgccgcag gcctttccga
     4801 gctggatact cgatccttcg tagacgtaga cagggatatc gatcccactc accatatttt
     4861 tgacggattc agggctgttc tcggccacaa caaccagtct ggccttgccc tcatcaatgc
     4921 attcccgggt cctgttccgg cccagaaaca ctgtaccagt cttcacggct ttacggagtg
     4981 aagcattaaa gtccattacg atttctccta ggttttcagg tttaatggtt ttgcgatgag
     5041 tttcacatca ccggtgccaa gctggatggg ctggcccacg ataacgttct cggtaacgcc
     5101 gttcagttcg tccacctcgt tcgtgattgc cgcatccagc agatggttta ccgtcacttc
     5161 gaatgcagca cgggaaagga cactctcctt ctcaccggcg ataccatggc ggccgatctg
     5221 cttgacctcg ccgtccatgc acatcatatc cgcaacgagc atgatgtgac ggacatcgac
     5281 gccgataccc tgttcattca gtgtggtcag ggcctcctgg atgattgcat tccggccggc
     5341 ctcgatgccg agcacatcgg cgatctcgct gatgttgttg ctccgggtgc gggaagtgtc
     5401 gactcctttg acttcgaaga catcttttag attggagccc tcagtataaa ggatatactc
     5461 tcccccctcc tttctgacaa cgacccgctc gatatcatcg atgccctgaa cgatgacgtt
     5521 cctgacgtgt tccgcaagtt gaaagaggtt ctggtaactt tcctggtttt tcggggtgaa
     5581 cgtgatcgaa ggctttttcg ggtcggcctc aacctcaaaa tcacggtaat gccgccgttc
     5641 ccggatcttt cttggcgcta tctcgatgat ctcgtccacc gcgatctttc gccggtcaca
     5701 gaccgccctg ttcaatagaa caagaacctg catattctcc atatctatgg tgatatcccc
     5761 aaattcatgg agcggcgcgg cctcaatctg ccagctcacc tcgcgggcgc ggtcgcgatc
     5821 gaacgcccag tcatccagca ggtagaccgc catcgtcggg gtgcttggtt cgcgcctggc
     5881 gtccatgatc tcgatgaggc gggggagacc cagcgtgacg ttgatctcgg ccacacccgc
     5941 gtagtggaac gtacgcatcg tcatctgggt gccgggctcg cctattgact gcgctgccac
     6001 aacacctacc gcctcgcagg gttcgatccg gctcttctgg tactcgttgg agaccatctc
     6061 cacaatccgc ttgaactgct ccgatgtgat ctccttcccc gcgagacttg ctttcaggtc
     6121 ctcccgcgtc cggtaaggca gatcgagggc gtcgatctcc cgttccatct cgctgttcat
     6181 ttgacctcct ccctcaacac gctctccacg atacccttaa cgtccaccgg ggcgccgtaa
     6241 ctgctctttg cagggtcggt accgtcctcg ccgtaacgga actggatgat ccggcctccg
     6301 gtagtccgga cagtgccgtc gtaggcgacc ttgaggtcct gcagggcatt gatcatcctc
     6361 cgctggagat aaccgctctg cgatgtccgg acggctgtgt ccacaagacc ctcacgtccg
     6421 ccgatggcgt ggaagaagaa ctccgtgggg gagaggccgc tcttgtaact gttggtgatg
     6481 aacccgtgcg ccttggcgcc gcgatcgccg cgccggaagt ggggcagcgt ccggtcctcg
     6541 tagccacgca tgatccgctc gccgcgaacc gactgctgcc cgagacatcc ggccatctgt
     6601 gtcaggttca gcatcgaccc gcgtgccccg gagacagcca tcacgacggc gctgttatcc
     6661 atccccaggc gctggccggc gatctcaccg gtacggtcac gggccttgcc gagcacctgc
     6721 atgatctgca tctcaagcgt ctcctcaagc gtccggcccg gcatgggctc aagttgcccc
     6781 tcgttgtaga tccgtatccg gcgctcgaca tcggtgatgg cgttgttgag cacctcctca
     6841 atctgtccgt actccgtcct ggagaggtct tcatcatcga tgccgaacga gaatccctcc
     6901 agcatgatcg cctggatcga gagtttggtc acgtcgtcga tgaacctggc cgctctcttc
     6961 ataccgtgct gccttatgat ccggtgcagg atctggccgt caaacgcgcc gatcgccttc
     7021 ttgtcgatgg ttcctgagag caggttgcca tcgacgatcc ggacgtatgc gtcgttctca
     7081 cagcgttcct gccggcatgt ctcgcagttc tggcacgacg aagctcgaaa gatcatgttc
     7141 agatcatcgg ggaggatgag cgagaagacc tgtttgtttg tccagagcgg gttctcgtcc
     7201 ccgtcctttg ctgcaggttc gggcaggtgg tcgatgggaa ggttccgggt gaggtagagt
     7261 gtcattgccc tgtcgaagtg gcggattttg tgagtcagga ggaatatgcc tgagatgtgg
     7321 tcatgtatac cgccgatgat cgggccgcct gttcgtggtg agagaatgtt tgcctgcacc
     7381 gcgatcagga tctcggcctc ggcccgcgcc tcttcagtct gcgggatgtg cagattcatc
     7441 tcgtcgccgt caaagtcggc attgtagggt gggcagacgg ctgggttcag ccggaacgtc
     7501 tttccatcca tcacgacaac ccggtgcgcc atgatgctca tccggtgcag cgagggctgt
     7561 cggttgaaga ggactatatc tccgtctttg atctgcctct ccaccgtcca gccaggctcg
     7621 agcatctctg tgaccgtttc caggttcacc tccgagagtc gtatccgccg gttgtccggc
     7681 cggatgacgt agtttgcgcc gcatggtgat cggattgtgg ggcgcacggg gccgttcagg
     7741 acgtaatgcc gcatctcctc gatgttgaac ggcgttaccc tgacggggat ggtcatctcg
     7801 tttgcgatag caagcgggat tccgacctcg gtgatcgaaa ggttcgggtc aggtgagatc
     7861 acggtacggg ccgagaagtt gactcgcttg cccgatagag agccgcgaaa tcgtccctct
     7921 ttccccttga gccgctgtga gagggtcttt aagggtcggc cgctccggtg ccgcgcaggc
     7981 gggcaacctg ccacctcgtt gtcgaggtag gtggtgacat ggtactgcag gagttcccag
     8041 aggtcctcga ttatgagctg gggtgcgcca gcatcctggt tctctttgaa ccgctggttg
     8101 atccttataa tatccacgag tttgtgggtc aggtcatcct cggatcgctg cccgttctca
     8161 aggatgatcg aggggcgcat cgtgaccggc ggaactggga gaacggtaag gatagtccac
     8221 tccggtctcg cgacgtccgg gttgatcccg agagcgcgca ggtcttcgtc cgggatcttc
     8281 tccaggcgtg cgcgtatgtc ggcgggggtc agtttgtgct ctatctttct gccgtcttcc
     8341 aaaaagacct cggagaaggt ggtgggtttc tcgaagttga tcttgagctg ctgttccccg
     8401 cagtgggggc agacccgctc tttcttgatg tccttctcgg agacaacctc tggcgtgagg
     8461 tcgctatcct cggtgccgac gatcttctcg acctcttcag cagtcatcag cagccttgaa
     8521 cacgatctgc aggttacacg caagagttta cggatcaacc gggtgtagcc tacatggatg
     8581 actggttttg caagttcgat gtgaccgaaa tggcccggac agtcggccgc cttctggtcg
     8641 caggtcttgc aacggagacc cgggtcgatg acaccaaggc tgaggtccat aagcccctgc
     8701 gggtagggaa acccgtcatc gtcataggta tccgcccaga tgatctttcg aacgctcatc
     8761 ttgcggatct ccttcggcga gaggagtccg aactcgatct ttccaacacg tttaggactg
     8821 gtcatggttt gaaactgccc ccattcttat accatatctt cgagcctcag gcggggagcg
     8881 atgcccatgc tcttcatctc gtcgaggagg agcttgaacg cgtagctcat ctcgacgggg
     8941 tatatatcgg tctcgccgcc gcaggtaaga cagcgggtaa ccttcctctt ccggtcgacc
     9001 atcgccacca taccgcattt cgcacagacc cactgggtca ccttatccga ctcatcgagg
     9061 agtcgctctt tgagtgccat ggcggcgccg tggccgatca tcacatcacg ctccatctca
     9121 ccgaaccgga gccctccctc acgggcacgg ccttcggtag gctggcgggt aagaacctgc
     9181 accggaccgc gcgagcgtgc atgcatcttg cttgagacca tgtggtagag tttctggtag
     9241 tagatgacgc cgatgtagat atctgccgca aactgccgtc cggtgacacc gtcgtacatg
     9301 acttcgcggc cgttatgcgc aaacccgagt gctttgagcg attttcgtag atctgcctct
     9361 cgttcacccc gaaacgccgt cgcgtcgatc cggcgcccat cgagtgaacc caccttgccg
     9421 cccagcatct cgagcatgtg cccgatggtc atacggctcg ggacggcgtg ggggttgatg
     9481 ataaggtcag gcgtcatgcc cgactcggtg aagggcatgt ctgctgcggg ctggatgagc
     9541 ccgatgacac ccttctggcc gtgccgggac gcaaacttgt cgccgacttc cggtatccgg
     9601 aggtcgcgag tcctgacctt taccagcttg gaggagttct ccccctccgt gatgatgacc
     9661 gtatccacga tcccgtgctc gttgcttcgc atcgtgaccg atgtatcacg ccgtttttct
     9721 acagcgatca gctcgcttgt aggctcctcc aggaacctcg ggggggatgt cttgccgatc
     9781 aggacatcct tctcacggac gaccgtctcg gggttgatga caccgtcaat gtccaggttc
     9841 gcgtagtatt cggaaccgtg tgctccggag acctcttcgt ccgggatctc gatccggtca
     9901 acctgaccac cggggtaacg ccgttcttcg ccctcgtagg tgcggaagaa gtgcgaccgc
     9961 cccaggcccc ggtcgatgga agcctggttg atgatcagtg cgtcctcaat gttgaaccct
    10021 tcataggaga ggatggcgac aacgaagttc tggcctgccg gccgatcgtc ggatccgatg
    10081 acatcggagg tctgggtgta gatgagcggt ttctgggcgt agtgaagcat gtgccccctg
    10141 gtatcggggc ggagtttcat gtttgctgcg ccgaacccga gcgcctgttt gaccatcccg
    10201 gctcccattg tgacacgggg gctggcattg tgctccggga acggaacgtg cgccgcaccg
    10261 atacccagta tgagggaggg gtcgatctcc aggtgcgtgt gttctgaggt gagatccttt
    10321 tcgtttatgg caatgaggag atcctcctcc tcttcggcat cgacaaactc gatcgcaccc
    10381 ttctgcacaa ggtcgtcaaa ggtgatccca ccgttccgaa ggcgttcgat atcctcttcg
    10441 gttaccgcgg gtaaaccatc tcgaaccacg atcagcggcc ggcgtgccct gccccggtcg
    10501 gtgtggacta tgacgtcgtt gttgaattcc ttgtaggaga tgtttatttc agaagagatc
    10561 ctgccgcacc ggcgcatctc gcgcaaccgg ctgacaaggt cccgggggtt gtctacaagt
    10621 ccaatcaggg ctccgtcaac aaagacacgt gactgtctca tgccgtctct ctccgcaggg
    10681 cgataacgcc catctcatac agagtgttct tcacttcctc ttcgttgctg acaccattgc
    10741 tgatctcgac catctgggcg aagttcttaa ccagcccaca gtttggccct tctggcgtct
    10801 cgcttggaca gatccgtccc cactgggttg gatgcaggtc acgggcctcg aaatggggtt
    10861 gagatcgtga gagcggggag atcacacggc ggaggtgcga tagtacagcc atgtggtcga
    10921 cccggtccag gagctgcgat acgcccgtgc gccctcccac ccagttgccg gtggcaagag
    10981 ggtgaagcag cctctctgta aggacgtccg cgcgcaccgc ggtgccgatc gagaggtcgc
    11041 gatggcgcat gctggcccgt tcgagctggt acttaacgtc ccgcgtcagg cggttgagcg
    11101 agatccggaa taggtcttcc ataaggtctc ctgcaagttt cagccgtttg ttggcgtagt
    11161 ggtccttatc gtctattctg cgccgctcaa gtaccaggtc gaaacaggcc tcggccatcc
    11221 gccccaggaa gtgcgctttt gccagacgga cactctcgac cgccgctctg taaccggggt
    11281 cgtcctcttt caagcccgcc ggcatcaggt ggtttaagtg ggggagcaga tagttgtcaa
    11341 gaacaaactc ggcccgtttg cgctggtagt cacgggtctg gttgggcgcg agcttcttgc
    11401 cgacgtacat gatcccttcg tcgacggtgt cacactcgct ctcctccagg ttctgcatca
    11461 taaatgtcag gatatcctca tcggttgaga ccgcctcgac gatgtcatgg tcaccctgga
    11521 gccccagcgc tcgcatcagg tcgacgaaac ggaggtgccc ggcgaccgag ggaaacgaga
    11581 cctctagcag gttcttgcgg tttcgctcca cgatcacgag cgcgcggtag ccacggtact
    11641 gcgaaaagac cttcgcaacg tatatccgtt cgttgtagcg ttcggtaaac tcggtcatga
    11701 tcttgtttga ggcaagatcc tcaagcgtca tcagcacccg ctctgtgccg ttgacgataa
    11761 agtagccgcc aggatcgagg gggtcttcac catgctcgat cctatctctg tcgctcaggt
    11821 tgtagaggtt gcaggctgcc gaacccacca tcaccggcag ctgcccgatg gtggtgacga
    11881 tcgggtcctg cggttctccg ccctggacga gcgtcatctc aagttgtatg ggcgcggcgt
    11941 aggtgaggtt ccgcaggcgc gcctcactcg ggaagagctc ggactgcgat ccgtccgctt
    12001 cacggacgag gggtttcttc acctctatcc ttcccagttc aacccataca gcctcttttc
    12061 ccttgccacg ggactcgatg tcggtctcga tgactctttg ttcatcgacg acttcctgaa
    12121 ggttgtgcag gaggaaattg ttataagagt ctaactggtg gcgcgcaaca tgttcccgcg
    12181 caaaatatgc cctggacaaa acacttctgt ctaacagatg gatcagctcc cggcctcaga
    12241 atttatttct tgggtctctt cacgactatt cggtaggctt cggccctacc agcggttcga
    12301 ctgtcacgaa tgatgcggat gacgtttcct acctcgcccc ggatggcctt cactgcaggg
    12361 tcgtcgtggt agatcttcgg taattgttct aaagatatac ggtaggtcgc aagaagttcc
    12421 gcaacctcat cctcgctcat gatctgatgg tcaggtacca tctcgtgttt gagtacgtcc
    12481 aatgaagtgc ccatcacctt acacctcatc aacgaaaaag ttgcatatca gatgcatcac
    12541 cttgctaaaa cccatagaca tcgcccatgg caacgggccc aaggggattt gaacccccga
    12601 ccacctggtt aaaagccagg cgctctacct aactgagcta taggccccgc gcgtactgcc
    12661 atccagcatt attacaatgc gagaagagta taaataactt acccgaagac gtggagtagt
    12721 gtttgcagtc tgatggtata aacctttcaa ttatccttca attctacgag tcataccgga
    12781 atattttaat gcttccgggc attcctgttt tgtacatact ttgccgatcc tggtgcatcc
    12841 atgattcctt cacgatacta tgccgacaat gccgtacccg ctatatcgtg ggttacgcag
    12901 ggcatctacc tcctcattgc cgtcgcgcta tccgtgcttg cgctcgtctc gttatatgac
    12961 gttcttctcc agatggtggc tctcattcac gccgaagaca tgatgcaggg tgtcctcgag
    13021 gtgctgcatg cggtgctctt gacccttatc ctggtggaga tcctggagac ggtgacagca
    13081 tatttcagga caaaaaaggt gctggtaacg ccgatcctga ttgcgggggt cactgcgatg
    13141 gtgcggcggg tgttgatctt cggggtagaa gtccacgaac ccgccgagat tggcatgaca
    13201 ctcgc
//
LOCUS       DAOSZX010000035        13082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_3997_length_13082_cov_19.014508, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000035 DAOSZX010000000
VERSION     DAOSZX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3997_length_13082_cov_19.014508"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(675..746)
                     /locus_tag="PK196_04415"
     tRNA            complement(675..746)
                     /locus_tag="PK196_04415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(711..713),aa:Gly,seq:gcc)
     gene            complement(849..1541)
                     /locus_tag="PK196_04420"
     CDS             complement(849..1541)
                     /locus_tag="PK196_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HON40696.1"
                     /translation="MVAVEPLLILALALAPGVFWAWYFYRRDRFEPEPAAMVLKIFIL
                     GLLVTFPVAFAEGFIGLFIASPLLMGAVVAPMVEEYGKFTIVRRFAYLHTEFDEPMDG
                     IVYAAAAALGLATLENVLYVFSAYLTSPSLAFSTVIVRAIFSVPGHALFAGVWGYALG
                     QARFAPAEKRPLLIRRGLVLGMALHGIFNLLLISAEIIAYAMAAFILVLTPGLWMLAN
                     RNIRQALRKGHR"
     gene            1739..2365
                     /locus_tag="PK196_04425"
     CDS             1739..2365
                     /locus_tag="PK196_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HON40697.1"
                     /translation="MIPLMLDLTGRRVLIFGGGDVGARKAAYFEHEAEVTVISRSFSP
                     RLDGLAVRRHETDLASLPDDAIGELLDGAFLAIAATPDPALNNRIGRLCASAGVLFNN
                     AAGKPGDVIIPSVVRGRNYLIAISTGGKSPAVSRYLRMRLESEYADLDRMIDLQEEMR
                     SILRETEPVQAERSRTLWKILLDDEIRELLALDYGRARELAIARYLHA"
     gene            2358..3629
                     /gene="hemA"
                     /locus_tag="PK196_04430"
     CDS             2358..3629
                     /gene="hemA"
                     /locus_tag="PK196_04430"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HON40698.1"
                     /translation="MPEPLMVPLAIAGVSHHTADIATLEAFRFPDETAFLREARERFR
                     GVLLLQTCNRVEVLVQGDGRSLEEFLHEMGRDGFTLIEGEAVPRHLLELAAGIDSLIV
                     GEDQILGQLKQALATTEEAGTSCAAIELCITKAVHAGVRVRRQTGINRGAVSVGSAAV
                     ALAEKLLGTLRDRHILVVGSGEMGVLVAQALAAKGLTAIYVANRTYDRAVMLAEKIGG
                     LAVNFKDLYRYIALSDVVISCTAAPHPVIRTEGVREAMKERLWPLDRQPRHLILIDIA
                     QPRDIEEGVREIEGVHLFTIDDLRSVNEAAMASRRDEANRARQIIEEELEHFIRLLRR
                     TAADETLALLYTWAESIRGRERDRALARLGRVDERTQAIVDDLTRALTKKLLADVTTA
                     IRTSAERGDLNTAETLIRAITRGESCFQNKE"
     gene            3608..4591
                     /gene="hemB"
                     /locus_tag="PK196_04435"
     CDS             3608..4591
                     /gene="hemB"
                     /locus_tag="PK196_04435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HON40699.1"
                     /translation="MFPEQRMRRRRRRIIQPLLRETELRKTDLIAPVFVDESIDAPIP
                     IASMPGQFRHPVDGVLDHCRRLMDSGIRAVILFGIPAEKDSEATAAYASDGAVQRAVR
                     GIKRGLGGMAVITDLCACEYTDHGHCGIVGETADGPDLLNDPSLDLMARIAVSHAESG
                     ADIVAPSCMLDGMVRTIRQALDSAGHTDTLIMSYSSKFASALYGPFRDAADSGFTFGD
                     RTTYQIYPGNAREAVMESELDSAEGADILMVKPAGIYLDILASVKRIGLPVAAYQVSG
                     EYAMIKAAAERGWLDERAVAIESLIAIKRAGADLIITYYAEDAARWLDEEQ"
     gene            4578..5825
                     /gene="hemL"
                     /locus_tag="PK196_04440"
     CDS             4578..5825
                     /gene="hemL"
                     /locus_tag="PK196_04440"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HON40700.1"
                     /translation="MKSSDLFSRAKQLMPGGVSSPVRAIRPYPFYVERAAGPRLMTVD
                     GMEFIDCCLGYGPLILGHAHPDVREAIMRQVEKGWLYGTPTPLEIDLAARIVDDHPAV
                     DMVRFVSSGSEATMAAIRAARGYTGKQDIVKVEGGFHGAHDAVLVKAGSGAATLGVPD
                     SAGVLADVAAHTRQVPYNDPEALETLLAGNEDVAAFIVEPIMGNIGPVLPEDGYLADI
                     REITAAHDTLLIFDEVITGYRVGIGGAQVRYGVKPDLATFGKIIGGGLPIGAFGGRRD
                     IMELVAPSGPVYQAGTFSGNPASLAAGYATLCWLHTHPEAYRHLDEAVRAIEEAAVDA
                     QRGSFVRMGSLFKHFFREAPPRNYRDVQECDREAFSRFWKAMLDAGVFLPPSQFETNF
                     ISTAHSRSDIDKIADAYRSCLFA"
     gene            5810..6694
                     /gene="hemC"
                     /locus_tag="PK196_04445"
     CDS             5810..6694
                     /gene="hemC"
                     /locus_tag="PK196_04445"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HON40701.1"
                     /translation="MSLRIGTRGSALAMAQTQKVLGLLADLGVDAEAVTITTEGDTAT
                     GVPLHAIGGQGVFVRALDDAILRGEIDAAVHSMKDIPAARPAGLACPAVLERDSAADF
                     LAHDAPLDEVRVVGSSSTRRRAQILRNDPALEVRELRGNVDTRIRKLREGRYDAIVLA
                     EAGLERLGLTLEGEPLPTEWFVPSPNQGAIAVVCRDDQAVVETIAALDHARTRLDAGI
                     ERAVMEEVGGGCFTPQGIYCRDTDLVAEVLSLDGSRWERIERRVTTIEEARECGRELR
                     EKAADLIEEAYAALGIRP"
     gene            6691..7431
                     /gene="cobA"
                     /locus_tag="PK196_04450"
     CDS             6691..7431
                     /gene="cobA"
                     /locus_tag="PK196_04450"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HON40702.1"
                     /translation="MTGRAYLVGAGPGGLGLMTIRAREVIESADVILYDQLPGEEILA
                     TLPRSAELIDCGKYGGNHTLEQHEIEALMVERVKEGKTVVRLKGGDPFLFGRGGEEME
                     TLRAHGVPVEVVPGVTSAIAVPEMVGIPVTHRRYASTVTFITGHEDPAKPESALDWEV
                     LSRLKGTLVILMGVKNLPKIAAALTAHGKDPATPVAVIERGLRPDQRVTVGTLADIAD
                     KARAAGVRPPAVIVIGGVVELYEGSGTT"
     gene            7481..9124
                     /gene="ilvD"
                     /locus_tag="PK196_04455"
     CDS             7481..9124
                     /gene="ilvD"
                     /locus_tag="PK196_04455"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HON40703.1"
                     /translation="MRSETVKRGYQRAPNRALLRALGITDREMEMPFIGIANAYNNIV
                     PGHIHLRSISQKVQEGIAAAGGVPFEFGTIGICDGIAMGHEGMRYSLPSRENIADAIE
                     LMVEAHRFDGLVCLGTCDKIVPGMLMAAVRCNVPTIVVTGGAMLPGFAAGRELSLIDV
                     FESVGRVAAGTMTEEDLGEIECAAMPGCGSCQGLYTANTMACVTEALGLSLPGCAAIP
                     AVDAAKLRIARESGERVVGLVREDLRPRNILTPGSLSNAIRVDMALGGSTNTVLHLMA
                     IAREAGIPLDLETFSAIGEETPHICHMMPGGPHSMLALYRAGGIPAVLKRLEHLLDDA
                     PTVSGRSILEIAKSARIADPEVIRSTDAPVSPAGGLKIVRGTLAPDGAVVKCAAVPEA
                     MWRHRGPARVFDGEDAAMNAILKREIAEGDVVVIRYEGPRGGPGMPEMLSPTSALMGL
                     GYARVALVTDGRFSGGTRGPCIGHIAPEAAVGGPIAFVKDGDEIEIDLYEKRLDLCLD
                     PQTLKERQKGWKPPERNITGVLARYARTVEQANHGAVQR"
     assembly_gap    9532..9631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9696..10277
                     /locus_tag="PK196_04460"
     CDS             9696..10277
                     /locus_tag="PK196_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HON40704.1"
                     /translation="MIERYLEGNRRFLEEDFRRDPGHYRPLASAQHPGALWIGCSDSR
                     VDPERITGAKAGELFVHRNIGNIVPVHDWNLATVIEYAVNHLKVDDIVICGHSNCGAI
                     KSLDHEGGDHYIPLWLNNAVEAKRRVDSRIPAPKTPEEEKNRLRLIEIENVGLQIEHL
                     RTYPPVRAAEKEGRIQIHGLYFDLATGELKKIF"
     gene            complement(10274..10618)
                     /locus_tag="PK196_04465"
     CDS             complement(10274..10618)
                     /locus_tag="PK196_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40705.1"
                     /translation="MAEVIVEIVGLKDSECGPFPCDETRSCGLETCHPSNNLLEAIGA
                     LREELFAEYGGRVVVKTTLIDEGMPEYVRRLIEEHHPPIPIILVNGRLTPIGRISLDL
                     IKEEIDSVLGTT"
     gene            complement(10647..11528)
                     /locus_tag="PK196_04470"
     CDS             complement(10647..11528)
                     /locus_tag="PK196_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON40706.1"
                     /translation="MSESAREEKTQTIETPEGTFTIRLTMDDMGSVYPGMIRYNIEVL
                     RGTETVYTYAINSYEIPPGSLFDARKTADIVFARLAQDVSSRPHLYLRPRVFTRPLPG
                     DTVDVVILQGSPRRFGNSAKIASWCDDEAARAGLSSRVFYLQDMDIRPCIGCYVCYDQ
                     GYCPVDDDMPRIIRALETASLIVVCTPVYTNTVPAPLKAAMDRCQWLHAREKVLGKEV
                     RARGLLIAVAGRRGKEAFACVTGVVRAFMGNLGIRFAEPILIGDLDRLRDVAEVEGIE
                     EEIRASLRVLLVSGDEG"
     gene            11657..12712
                     /gene="carA"
                     /locus_tag="PK196_04475"
     CDS             11657..12712
                     /gene="carA"
                     /locus_tag="PK196_04475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HON40707.1"
                     /translation="MKAVLGLEDGTFVVGDGFGVEGRCCGELVFTTQMTGYMEALTDP
                     SYAGQILMFTYPLIGNYGVDLQNFESPRVQAAGCVVREIARVPTTKPSIADYFEENAL
                     FGISGVDTRSLTIKTRTCGTLRASLIVGGEDGNEAVRLARSAPPITGTDLIERVSCTA
                     PYTISGPGKRIALIDLGVKRHIIESLRYRGAEIHVFPHNAAADDLMAAEPDALFISNG
                     PGDPKRAAGAIRCVGELAGELPIIGICMGIQVAALALGAETYKMKFGHRGTNQPVRYR
                     DGKIYITTQNHGFAVDEESLPEGCTVSYRNVNDGTVEGFENSDLNITCVQFHPEAHGG
                     PRDTEMHFFDRIYREIP"
     gene            12712..>13082
                     /locus_tag="PK196_04480"
     CDS             12712..>13082
                     /locus_tag="PK196_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HON40708.1"
                     /translation="MPKMPHINKVLLIGSGPIQIGQAAEFDFSGSQACRALREEGVEV
                     VLVNSNPATIQTDPDMADTIYIEPLKAGIIAKIIEKEKPDGILSGMGGQTGLNLTAEL
                     AEMGALKGVEILGTPLEAIYHG"
BASE COUNT         2642 a         3939 c         4061 g         2340 t
ORIGIN      
        1 cagaaggata ccgcaaagca cccatttgcg tgataaaggt cccgggacgt tttcccggcg
       61 ggggggccac ctgcccaccc taaaccgggg ccaggcggcc gggaggcggg ggtaatcgag
      121 cgagagaggt tttttggtga cgtttccagt gacacgttga gacaggggag gggggagccg
      181 tatccgcctc cccctccccg tcagccccac ccccgcgggg gtgatatccc atcgatacac
      241 tgccacggga ggagataacc tctctgccgt cagtgtgaac acccttgact gaaatcctgt
      301 tcctgccccc cggacacggt atccggggct atcgccaatc actgcccgcc cccttgcagg
      361 gggcgggagg aggcgcggaa cgcgccgggc ggtgggggtg atctacgtaa accgttcaac
      421 cagagatcca ggaagactgg ggggggcgtt ccgccccccc ccatcagccc cacccccgcg
      481 ggggtgattc tccgtcgata cggtgccaga ggggagcatc aaccacagtg ttggtggatc
      541 ccttcatcaa gatcctgttt cctgtccatc agcacaatat cctggactta tcataattca
      601 ctgcccggct acggtctaca gatggcaagt tatccgtgtg cacgagaaga agagattcca
      661 gcctgacgct ggaatgcgcc gaccgggact cgaacccggg tttaggcgtt ggcaacgcct
      721 agtgataacc actacactat cggcgcacgc ctgtgctttc gcactgtgtg cctaaaagaa
      781 tgagtttttc aggctaataa gcctatcgaa gacctgcccg ctgcggcggt tgtgcaccgc
      841 cgctcgtctc aacggtgccc cttccgcaga gcctgcctta tgttccggtt cgcaagcatc
      901 cagagaccag gtgtcaggac caggatgaac gcggccatgg cataagcgat gatttctgcg
      961 gagatgagca ggagattgaa tatgccgtgc agcgccatcc ccagaacgag cccccgacgg
     1021 atgagcaggg ggcgcttctc ggcgggcgcg aaccgggcct gaccgagagc atagccccag
     1081 actccggcaa agagcgcatg ccccgggacc gagaagatcg cacgcacgat aaccgtgctg
     1141 aacgcaagtg agggagaggt caggtaagcc gaaaaaacgt agagaacgtt ctcaagcgtt
     1201 gccagcccga gcgcagcggc agcggcatag actatgccgt ccatcggttc gtcaaactcg
     1261 gtgtgcagat aggcaaaccg gcgcacaatt gtaaacttgc cgtactcctc gaccataggt
     1321 gctacaacgg cgcccatgag aaggggggat gctatgaaga gaccgatgaa tccctcggcg
     1381 aaggctacag gaaacgtgac caggagaccc aggatgaaga tcttcagaac catggccgcc
     1441 ggttctggct cgaatctgtc cctccggtag aagtaccacg cccagaacac acccggcgcc
     1501 agtgccaggg caaggatcag aagcggctcg acggcgacca cacatattga ttcatctcct
     1561 ggagagtaag agcctttccg cgcggcattt cacccatctt caaaaccagg tataaaaaag
     1621 tataaacttg accgggaaca ggtctcaggg agaggtgcta ccggtggatc caccaatttt
     1681 ttggtgaaac cggcttttca acaccttaat gtagtctatt caccacacta tagaacaaat
     1741 gatccctctc atgcttgatc tgaccggcag gagggtgctc atcttcggtg gaggcgatgt
     1801 aggtgcccgg aaggctgcgt acttcgagca tgaggcggag gtgacggtga tcagtcgttc
     1861 tttctcacca cggcttgacg gtcttgcggt caggcggcac gagaccgacc tcgcctctct
     1921 cccggatgat gcaatcgggg aactcctgga tggcgcattc cttgcgatag ccgcaacacc
     1981 cgatccggcc ctcaacaacc gtataggaag actctgcgcc tcggccggcg tcctcttcaa
     2041 caacgccgcg ggaaaacccg gcgacgtcat catcccctcg gtcgtccggg gacgcaacta
     2101 ccttatcgct atcagcaccg gcggaaagag ccctgccgta tcacgttacc tccggatgag
     2161 gcttgagtcg gagtatgcag acctggatcg gatgatcgac ctgcaggaag agatgcgctc
     2221 aatcctccgg gagaccgaac cggtgcaggc agagcgatca cgcaccctct ggaagatcct
     2281 gctggatgac gagatccggg agttgctggc ccttgattac ggccgcgcac gtgaactcgc
     2341 gatcgcgagg tacctccatg cctgaaccgc tcatggtccc cctcgcgatc gcgggcgtca
     2401 gccaccacac cgccgacatc gccacgcttg aggcgttcag gttccctgac gagacagctt
     2461 ttctccgcga agcgcgggaa cggttccgcg gcgtgctcct gctccagacc tgcaaccgcg
     2521 ttgaggtgct ggtgcagggg gatggacgta gccttgagga gttcctgcac gagatgggga
     2581 gggacgggtt tacgctcatc gagggcgagg ccgtgccccg ccacctcctg gaactggccg
     2641 ccggaatcga ctccctgata gtcggcgagg accagatcct gggtcagctc aaacaggcgc
     2701 ttgcaaccac ggaagaagca ggaacatcct gtgctgcaat cgaactctgc atcaccaagg
     2761 ccgtgcatgc aggtgtcagg gtccggcgcc agacagggat caaccgcggc gcggtctcag
     2821 tcggttccgc tgcggtggcg cttgcagaga aactccttgg caccctgcgc gaccggcaca
     2881 tcctggtggt aggcagcggg gagatggggg tgctggtggc gcaggccctg gccgcgaaag
     2941 gcctcaccgc aatctacgtc gccaacagaa cctatgaccg cgctgtgatg cttgcagaga
     3001 agatcggggg actggcggtc aacttcaagg acctctaccg ctacatcgct ctctccgacg
     3061 tcgtcatatc gtgcaccgcc gcaccgcacc cggtaatccg gacggagggt gtccgtgagg
     3121 ctatgaaaga acgcctctgg ccgctcgacc gccagccccg ccacctgata ctgatcgata
     3181 tcgcccagcc gcgcgacatc gaggagggcg tgcgcgagat cgagggcgtc cacctcttca
     3241 ccatcgatga cctccgtagc gtcaacgagg ctgccatggc ctccaggagg gacgaggcca
     3301 accgggcacg ccagataatc gaggaggaac ttgagcattt catccggctc ctccgccgga
     3361 cggcggccga cgagacgctc gccctcctct acacctgggc ggagtcgatc cgtggacgtg
     3421 aacgcgatcg tgcgctcgcc cggctcggga gggtggacga acgcacgcag gcgatagtcg
     3481 acgacctgac gagagcgctc accaaaaaac tcctggcgga cgttaccaca gccatccgga
     3541 caagcgcgga acgcggcgat ctaaacacgg cagagaccct gatacgggcg atcacccggg
     3601 gagaatcatg tttccagaac aaagaatgag gcggaggcgc agacggatca tccagccgct
     3661 cctccgcgag acagaactgc gaaagactga cctgatcgcg ccggtctttg tggacgaatc
     3721 gatagatgca ccgatcccga tcgcctcgat gccggggcag ttccgccacc cggtggacgg
     3781 cgttctcgac cactgccgcc gtctcatgga tagcggtatc cgggcggtca tcctctttgg
     3841 catcccggct gagaaagaca gtgaggcgac cgcggcatac gcaagcgacg gcgccgtcca
     3901 gcgggctgtc agggggatca agagagggct tggaggtatg gcggtcataa ccgatctctg
     3961 cgcctgtgag tacaccgatc acggccactg cgggatcgtc ggggagaccg ccgatggccc
     4021 cgatctctta aacgacccct cgcttgacct gatggcgcgg atcgccgtct cacacgcaga
     4081 aagcggcgcc gatatagtcg cgccttcctg catgctcgat ggcatggtca ggacgatccg
     4141 gcaggctctc gactctgccg ggcatacaga caccctgatc atgtcctact cctcgaagtt
     4201 tgcaagcgcc ctctacggcc cattccgcga tgcagcagac tccgggttta cgttcggcga
     4261 caggaccacc taccagatct accccgggaa cgcacgcgag gctgtgatgg aatcggaact
     4321 cgacagcgct gagggtgctg acatactgat ggtcaagccg gccgggatct accttgatat
     4381 cctggcctcc gtgaagcgga tagggctgcc ggttgccgcc taccaggtca gcggggagta
     4441 tgctatgatc aaagccgctg cagaacgcgg ctggctggac gagagggcgg tcgccatcga
     4501 gagcctcata gccataaaac gggcgggggc cgaccttatc ataacatact atgcagaaga
     4561 tgcagcgagg tggcttgatg aagagcagtg acctcttttc ccgtgcaaag caactgatgc
     4621 ccggcggggt aagcagcccg gtcagggcga tcaggccgta cccgttttac gtggaacgcg
     4681 cagcggggcc gcgtcttatg accgtcgatg gtatggagtt catcgactgc tgtctcggtt
     4741 acggccccct catactcggc cacgctcacc cggatgtgag ggaggcaatc atgcgccagg
     4801 ttgagaaggg gtggctctac ggcaccccga caccgcttga gatagacctt gccgcgagga
     4861 tcgtcgatga ccatccggcg gtagatatgg tccggttcgt ctcttcaggt tcagaagcca
     4921 cgatggctgc gatccgcgct gcccgcggct acaccggaaa gcaggatatc gtcaaggtcg
     4981 agggggggtt ccacggcgcc cacgacgctg tcctggtgaa ggccggttcc ggggcggcga
     5041 cccttggagt gcccgactcc gcaggcgtcc ttgccgacgt tgctgcgcac acccggcagg
     5101 ttccatacaa cgaccccgag gcgcttgaaa ccctgcttgc cgggaacgag gatgttgccg
     5161 cgttcatagt ggagcccatc atgggaaaca tcggccccgt cctgcccgaa gacggttacc
     5221 ttgctgatat ccgggagatc accgccgcac acgacaccct cctgatcttt gatgaggtga
     5281 tcaccggcta ccgggtcggg atcgggggtg cgcaggtgcg ttacggggtt aaaccagacc
     5341 ttgcaacatt tggaaagatc atcggtggcg ggcttcccat cggagcgttc ggggggcggc
     5401 gcgatatcat ggaactggtc gcaccctcag gcccggtcta ccaggccgga accttcagcg
     5461 gcaaccctgc aagccttgcg gcggggtatg caacgctctg ctggcttcac actcacccgg
     5521 aggcctaccg gcacctggat gaggcggtgc gggcgatcga ggaggccgca gtggacgcgc
     5581 agaggggctc atttgtcagg atgggctcgc tcttcaaaca cttcttcagg gaggcgcccc
     5641 cccggaacta cagggacgtc caggagtgtg acagggaggc gttctcgcgg ttctggaagg
     5701 cgatgcttga tgccggggtc ttcctcccgc cgtcacagtt tgagacgaac tttatctcca
     5761 ccgcgcacag cagatcggat atcgataaga ttgcggacgc ttacaggtca tgtctcttcg
     5821 cataggcaca cgggggagcg ctcttgctat ggcgcagaca cagaaggttc ttggcctgct
     5881 tgctgacctc ggcgtcgatg cagaggctgt caccatcaca acggagggcg acaccgcgac
     5941 cggcgtcccg ctccatgcca tcggggggca gggggtcttt gtccgggccc tcgacgacgc
     6001 catcctccgc ggcgagatcg atgctgctgt gcacagcatg aaagatatcc cggcggcgcg
     6061 tccggcaggg cttgcatgcc ctgcggtgct tgagcgtgac tctgccgccg attttctggc
     6121 gcacgacgcc ccgctcgatg aggttcgcgt cgtcgggtcg tcgagcacaa ggcgccgcgc
     6181 ccagatcctc cgcaacgacc cggcgcttga ggtgcgagaa ctgcgcggga acgttgatac
     6241 ccggatccgg aaactccggg aggggcgcta cgacgccatc gtgcttgccg aggcgggtct
     6301 tgaacggctc gggctgacgc tggagggcga accgcttccc acagagtggt ttgtcccctc
     6361 gccgaatcag ggggcgatcg ctgtggtctg ccgcgatgac caggcggtcg tcgagacgat
     6421 tgcagcgctt gaccatgccc ggactcgtct tgacgccggg atcgagcgcg ccgtgatgga
     6481 ggaggtcggt ggaggctgtt tcaccccgca ggggatatac tgccgggaca cagacctggt
     6541 tgccgaggtg ctctcgctcg atggctcccg gtgggagcgt atcgagcggc gcgtcacgac
     6601 gatcgaggag gcacgtgagt gcggccgaga actgcgcgag aaggcggccg acctgatcga
     6661 ggaggcctac gcggcactgg ggatacggcc atgacaggga gagcctacct ggttggggca
     6721 ggacccggtg gtctgggcct catgacgata cgcgcccgtg aggtgatcga gagcgcggat
     6781 gtgatccttt atgaccagct gccgggtgaa gagatcctgg cgaccctccc caggagcgcc
     6841 gaactcatcg actgcgggaa gtacggcggg aaccataccc tggagcagca cgagatcgag
     6901 gcgctgatgg ttgagcgggt gaaggaggga aagactgtcg tgcgcctgaa aggcggcgac
     6961 cccttcctct tcggccgcgg gggggaggag atggagacgc tccgggcgca cggcgtccct
     7021 gtcgaggtcg tgccgggggt gacgagcgcc atcgccgtcc cggagatggt cgggatcccg
     7081 gtcacccacc gacggtacgc ctcgaccgtg acattcatca cggggcacga ggatccagca
     7141 aaacctgagt ccgccctcga ctgggaggtc ctctcgcggc tgaaggggac gctcgtcatc
     7201 ctgatggggg taaagaacct cccaaaaatc gcggcagccc tgaccgcgca cgggaaggat
     7261 cctgcgaccc cggtggcggt catagagcgg gggttacggc cggaccagcg cgtgacggtc
     7321 gggacgcttg ccgatatcgc cgataaagcc cgtgcggcgg gtgtcagacc gccggcggtg
     7381 atcgtcatcg gcggggttgt ggaactctac gagggctccg gcaccacgta accggcatac
     7441 acttttcccc ctctgcgctt atcctcctat gaggtttaga atgcggagtg agacggttaa
     7501 gaggggttac cagcgtgccc cgaacagggc gctgctcagg gcgcttggca tcaccgaccg
     7561 cgagatggag atgccgttca tcgggatagc aaacgcctac aacaacatcg tcccggggca
     7621 catccacctc cggtcgatct cgcagaaggt gcaggagggg atcgcggccg cgggaggggt
     7681 gccgtttgag ttcgggacaa tcggcatctg cgatgggatc gcgatggggc acgaggggat
     7741 gcggtattcc ctcccctcgc gggagaacat cgctgacgcc attgaactga tggtcgaggc
     7801 gcaccgcttc gacggcctgg tctgtctcgg gacatgcgac aagatcgtcc ccgggatgct
     7861 catggcggcg gtgcggtgca acgtcccgac gatcgtcgtc acgggggggg cgatgctccc
     7921 cggtttcgcg gctggccgcg aactctctct catagacgtc ttcgagagcg ttggccgcgt
     7981 tgccgccggc acgatgaccg aagaagacct tggcgagatc gagtgtgcgg cgatgcccgg
     8041 gtgcgggagc tgtcagggac tgtataccgc aaacacgatg gcatgcgtga ccgaggcgct
     8101 ggggctctcc ctcccggggt gtgcagccat cccggcggtc gatgccgcta aactccgtat
     8161 agcccgtgag agcggggaac gcgttgtcgg cctggtgcgg gaagatctgc ggccacgcaa
     8221 tatcctcacc ccggggagcc tttcaaacgc tataagggtt gacatggcgc tcggcgggtc
     8281 gacgaacacc gtgcttcatc tgatggccat tgcgcgggag gcgggcattc ctcttgatct
     8341 tgagacattt agcgccatcg gtgaggagac gccgcacata tgccacatga tgcccggtgg
     8401 cccgcactcg atgcttgccc tctaccgcgc cgggggcata ccggcggtct taaagaggct
     8461 tgagcacctc ctcgacgacg ccccgacggt ctccggccgg tctatactgg agatcgcaaa
     8521 gagcgccagg atcgctgacc cggaggtgat aaggtcgacc gatgccccgg tcagccccgc
     8581 cggcggtcta aagatcgtcc ggggaacgct cgcccccgac ggcgctgtcg tcaagtgcgc
     8641 cgctgtcccg gaggccatgt ggcgtcaccg gggtccggca cgtgtcttcg acggcgaaga
     8701 tgccgcgatg aacgcgatcc ttaagcgcga gattgcggag ggcgacgttg tcgtgatccg
     8761 ctacgagggg ccgcggggag gccccgggat gccggagatg ctctcaccga catcggcact
     8821 gatggggctt ggttatgcca gggttgcact ggtcacggac ggccggttct ctggcggcac
     8881 aagggggcca tgcatcgggc atatagcacc cgaggccgcg gtcggcgggc ctatcgcctt
     8941 tgtgaaggac ggcgatgaga tcgagatcga cctctacgag aagcgactcg acctctgtct
     9001 cgatccgcag acgctgaaag agcggcagaa gggctggaaa ccaccagaac gcaatatcac
     9061 cggcgtgctc gccaggtatg ccaggacggt cgagcaggca aaccacggcg ccgtccagcg
     9121 gtgagggttt ccccttgacg gcggagggga cgctccctct ctcctgaata ccgtcttttg
     9181 aggtgaggaa tcgacccccc cacctccctc ccggccgctc cacggcctcc tcccgccccc
     9241 tcaagagggg gcgggcgagt gtctggtgat tgacccggat agagtgcacg gggagcagaa
     9301 aggggatctc ggtgaggtga tcgcatcgaa ccgcggatct tctcatcccc ccatggcacc
     9361 gcatcgacgg agaatcaccc ccctgggggt ggggctcgcc cctgtctcgg cgtgtcactg
     9421 gttgagcggt attcaaagat ccacctgtat acatttttaa cccccccgga acaacccact
     9481 cttctgccag gtgatggatg gagatgtgat cgtccacacc gcataaggag cnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncactcttct gccaggtgat ggatggagat
     9661 gtgatcgtcc acaccgcata aggagcagaa gagagatgat agagagatac cttgaaggga
     9721 acaggcgttt cctcgaagaa gacttcagaa gagatcccgg ccactacagg ccgctcgcat
     9781 cggcccagca ccccggtgcc ctctggatcg ggtgctccga ctcgagggtg gacccggagc
     9841 ggatcaccgg tgcaaaggcc ggagaactct ttgtgcaccg caacatcggc aacatcgtcc
     9901 cggttcacga ctggaacctg gcaaccgtga tagagtatgc agtgaaccac ctcaaggtcg
     9961 atgatatcgt catctgcgga cactcaaact gcggagcgat aaagtcgctc gaccacgaag
    10021 ggggcgacca ctacatcccg ctctggttaa acaacgcggt tgaggcgaaa cgtcgtgtcg
    10081 attcccggat cccggccccg aagacccccg aagaagagaa gaacaggctt cgtctcatcg
    10141 agatcgagaa cgtcggcctg cagatcgagc atctccgcac ctatcctccc gtcagggccg
    10201 cagaaaaaga ggggcggatc cagattcacg gcctttactt cgaccttgcc acaggcgagc
    10261 tgaagaagat attttaggtg gttccaagaa ccgaatcaat ctcttctttt atcaggtcga
    10321 gcgagatcct cccaataggc gtgagcctgc cgttgaccag gatgatcggg atcggcgggt
    10381 ggtgctcctc gatgaggcgc cgcacgtatt caggcatccc ttcatcgatc agagtcgtct
    10441 tcaccaccac cctccctcca tactcagcga agagttcttc gcgcagggcg ccgatggcct
    10501 ccagcaggtt gtttgagggg tggcaggtct caaggccgca ggatctcgtc tcgtcacagg
    10561 gaaagggccc gcactcggaa tctttgagcc cgacaatctc gactatcact tctgccatgc
    10621 atcaacggtg aggggcgtat gctatattat ccttcgtccc cggagacgag cagaacacgc
    10681 agcgaagccc tgatctcctc ttcaatccct tcgacctctg ccacgtcccg cagccggtcg
    10741 agatcaccta tcaaaatcgg ctcagcgaaa cgtataccca ggtttcccat gaacgccctg
    10801 accactcccg tgacgcaggc gaacgcctcc ttcccccgcc gccccgcaac cgcgatgagc
    10861 agaccgcggg ctcgcacctc ttttcccagc accttctccc gtgcgtggag ccactggcac
    10921 ctgtccatcg ccgccttcag gggtgcagga accgtgttgg tgtagaccgg cgtgcagaca
    10981 actatgagcg atgcggtctc aagcgcacgg atgatccggg gcatatcatc atccacagga
    11041 cagtaaccct ggtcgtagca gacgtagcag cctatgcacg gccgtatatc catatcctgc
    11101 aggtagaaga cacgggagga gagacctgcc cttgcggcct catcatcgca ccaggacgca
    11161 atctttgcgg agttgccgaa cctgcgggga ctcccctgca ggatcacaac atcgaccgta
    11221 tcccccggca gcggccgcgt aaagacccgc ggcctgagat agaggtgcgg gcgcgacgag
    11281 acgtcctgcg cgagcctggc aaagactata tccgccgtct tccgggcatc aaagagcgaa
    11341 ccaggcggga tctcgtaaga gttgatcgcg taggtgtaga ccgtctccgt gccgcggaga
    11401 acctcgatgt tgtagcggat catgccgggg tagacgctac ccatatcatc catggtcagc
    11461 ctgatggtga acgttccttc aggtgtctcg atagtttggg tcttctcttc tctcgcgctc
    11521 tcgctcatcg tctctcatct cgtcttgtcc tatattctac atttcggatt acctgaggta
    11581 tgcgccgttt tccggacccg catccgcctg gtcactaccg ttaataagga atccgatcca
    11641 catgatatgg taattcatga aggcggttct gggtcttgaa gacggaacat ttgttgtagg
    11701 ggatggtttt ggtgttgaag ggagatgttg cggggaactc gtcttcacga cgcagatgac
    11761 cggttacatg gaggcattga ccgatcccag ttatgccggg cagatcctga tgttcaccta
    11821 ccccctcatc gggaattacg gtgtagacct acagaatttt gagagccccc gggttcaggc
    11881 ggctggctgt gtcgtgcgcg agatcgcgcg cgtgcccacg acaaagccat caattgcaga
    11941 ctactttgaa gaaaacgctc tttttgggat atcgggtgtc gatacccgca gcctgaccat
    12001 aaagacacgg acatgcggca cactcagggc atctctcatc gtcggtggcg aggacgggaa
    12061 tgaggcggtc aggcttgcaa ggtcggctcc cccgatcaca ggaacggatc tcatcgagcg
    12121 tgtctcctgc accgcaccat acaccatcag cggccccgga aaacggatcg ccctcataga
    12181 tctcggggtg aaacgccaca tcatagagag tctccgctac cggggcgccg agatccacgt
    12241 cttcccgcac aacgcagcag cagacgatct gatggctgca gaacccgacg ctctcttcat
    12301 cagcaacggc cccggcgacc cgaaaagggc ggcgggggcc atcaggtgtg tgggagaact
    12361 ggccggagag ttgcccataa tcgggatctg catgggtatc caggttgcag cccttgcact
    12421 cggcgcagag acctacaaga tgaagttcgg ccaccggggg acgaaccagc ctgtccgcta
    12481 ccgcgatggg aagatctaca tcaccacgca gaaccacggc ttcgccgtgg acgaggagag
    12541 cctgccggag ggctgcaccg tctcctaccg gaacgtgaac gacggaacgg tcgaggggtt
    12601 cgagaacagc gatctcaaca tcacctgcgt ccagttccat ccagaggcgc acggtgggcc
    12661 gcgagatacg gagatgcact tctttgaccg tatatacagg gagattccat aatgccaaag
    12721 atgcctcata ttaataaggt cctccttatc ggctctggcc ccatccagat aggacaggcg
    12781 gccgagtttg atttctcggg atcgcaggcc tgccgcgccc tccgggaaga gggtgtcgag
    12841 gtcgtcctgg tcaactcaaa ccccgcgacg atccagaccg accccgatat ggcagacacc
    12901 atctacatcg aacccctgaa ggccgggatc atcgcaaaga tcatcgagaa ggagaagcct
    12961 gacggtatac tgagcggcat gggcggtcag accggtctca acctgaccgc ggaactcgca
    13021 gagatgggcg ctctcaaggg cgtcgagatc cttggaacac cgcttgaggc gatctaccac
    13081 gg
//
LOCUS       DAOSZX010000036        13017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4031_length_13017_cov_21.215553, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000036 DAOSZX010000000
VERSION     DAOSZX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_13017_cov_21.215553"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2208)
                     /gene="hypF"
                     /locus_tag="PK196_04485"
     CDS             complement(<1..2208)
                     /gene="hypF"
                     /locus_tag="PK196_04485"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HON40709.1"
                     /translation="MRRSGTIIIRGIVQGVGFRPFVYAKAREFGITGTVKNLGSEVEI
                     HASGERFEEFLEAVSRGTPLSRIDSVDVYNLRDGQETAGFTILESGRGSLTGFIPADV
                     AICDECIADIFAPGGRYEGYWATSCVNCGPRYSIITDVPYDRERTSMAEFPLCPACQS
                     EYSDPSSRRHHAQTIACAACGPRLSLLRADGRPVAAEDPIREAAALLDEGSIVAIRGI
                     GGFHIACIEEAAEELKSRLGRTEQPLAVMAEPEEVERIAVVSPDDRRILHSRERPIVV
                     LEKRDPESHRTISRLHTIGCMLPYTGLHYLLFAHLDHRLLVMTSANLPGYPMITDLES
                     ALERLPRQVDYILTHNRRIVNRCDDSVVRDGCIIRLSRGLAPKREAIDLGDACILGVG
                     PELNSNISVYKNGFCITSPHIGNVRNPQTLAYLTETVEKIGKLTDARYDMVVHDLHPQ
                     FLSTRYAREVAEANGAELLAVQHHRAHIAAATREECVGIAIDGVGYGDDGTVWGGEVF
                     AGEVPHLERVAHLEVVPMPGGDLATRFPERMLYGILPTAEIRGLLASRGWSDMELAVL
                     AKQVERGLNVAATSSTGRVLDAASALLGICRERTYDGEPAMELESEAYGGRASVWDLE
                     FRQDGGCEVLSTRSLLEEAYRRMVAGERVADIAASIQFNLARGITTMAVRAAEERGIP
                     RVVLSGGVAYNRAIRETIGAGVMAAGLEFIVSREYPLGDGCISFGQVVYGGSMR"
     gene            2266..2778
                     /locus_tag="PK196_04490"
     CDS             2266..2778
                     /locus_tag="PK196_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HON40710.1"
                     /translation="MTGHIKILNDPVELVPLLITFNNAEYKKIYDLLNKAWMTEEDLA
                     EYVGKPTVAECLAILKKGNLIEEQWRMPKPGEKPTKEYRATYSKFRASFQCSMGDIGD
                     LLHIAISNDESLRSIVDAVQQEIMTGTTSIGDISRKYGVSPIFIKGLAKRIPGLDVRG
                     QGMVLLDRTQ"
     gene            2762..3571
                     /locus_tag="PK196_04495"
     CDS             2762..3571
                     /locus_tag="PK196_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HON40711.1"
                     /translation="MTEPSEDPLYVILRSKREATRFQILVEIAEHQPAIRQQEIAGKL
                     GVTPQAVSEYIRELVDEGLVTAHGRGRYEVTKSGVEWVLRHAEALESYARHVTRDIIQ
                     QVAVWTAIAEDDIRKGDTVGVFMRKGWLYATEREQSAMGVATMDARPGEDLGVARLNG
                     IIEHKEGAVHVCKVPRVERGGSRMVDLDLLRDVVRDAEVVAAVGLESYVALKKAGIEP
                     DMFFGAREGVIEAAFHGLECAILIVDEEFTDFLKRLETVGLAYTIHDLIPS"
     gene            3568..5580
                     /locus_tag="PK196_04500"
     CDS             3568..5580
                     /locus_tag="PK196_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON40712.1"
                     /translation="MKIIVQPQKSAYKLLFVEGDRVEGTGFVTLTATQKGQRPKNFKM
                     RRRGSRHPAPVPTRDLITMLRRSSVALAAESPEFESFLADLQIPSSRLYICRFCQLED
                     RFAPLDKTTGVRYGGEWICMECAKREMRRELGYMGRFGGSVLIHLEKLLAQVRDLDRV
                     LASVGPERPDRQRTLFDRLEAHEIEKTLHITDLSLPPAFVKAAGVDYLMPVQHLAVQS
                     GLLEGQHLLVVSATASGKTFIGEMAGMKNFLEGRGRMLFLVPLVALANQKYQRFRDRY
                     GHLVRVALQVGVSRLNLPETRPAGDRDVNAPVVVGTYEGIDHLLRTGKTLKDIGTVVI
                     DEVQMLEDAERGHRLDGLIARLKYIAPDAQFLYLSATIGAPGLLAEKLGANLVRYAER
                     PVPLERHLIFIEREKKIAFIKQMVAEEYRMRSSKGYRGQTIVFTNSRARCHVIADALG
                     KMAVPYHAGLSAQERREVERRFSNGEVAAVVTTAALAAGVDFPASQVIFDALAMGIQW
                     LTVQEFHQMMGRAGRPDFHDLGRVVILAEPGGSYSRTSGKTEEEVAIALLRGEMEEVA
                     PEYDLEQSTEEFVANAVVCGGDEQQIIRMNRMMVGTLEPALPTLLDHGLVRRRGGRIE
                     LTDMARVMAEHFIGAERLILVRDLVRRMDDAAGIVAELECAAEEEQ"
     gene            complement(5930..7153)
                     /locus_tag="PK196_04505"
     CDS             complement(5930..7153)
                     /locus_tag="PK196_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40713.1"
                     /translation="MKTARRIYNVLFISVFATMLGLGVVSPLLPLYAENLGATGIWLG
                     IIFSAFALSRSIFMPIIGRVSDRQGRKWIILIGMFAYAVLSLAYIIADSVYSLTVVRF
                     AHGIASAMVVPIAMAYIADLSEHGKEGSYMGNFSISMFLGMGVGPLLGGFLNDAFGMN
                     SVFYTMAALSAFATLLVAVSLPEAKRASSAIKGGEPAPMRDIFRLPVMRGVMVFTLIS
                     ALGRGGTMVFIPVFAPLIAISPSSVGVILSVNTFIMALLQVPVGKFTDRGNKVALIVV
                     GSAIAAIALAIIPFSRSFWDLLVITSLIGVGGAIQQPSIMALTVDAGRTLGMGTSMGA
                     YNTIFGIGTIIAPLMGGLFMDYIGIDAVFYAAGAISLLGTAVFAVMMQKAAGEINGGQ
                     NAGMVAGPAPDDDPR"
     gene            complement(7271..8101)
                     /locus_tag="PK196_04510"
     CDS             complement(7271..8101)
                     /locus_tag="PK196_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40714.1"
                     /translation="MRVADILEVEDCRLIEPSFADLTLDSCTDYLFQVRADEARLLVD
                     EDEEVIALALHDGRGWHVASFLYRDPNLAVIDYFEAIGGDIYQERRETWARAVRDYYS
                     MELLETTSPALEDLRPGRAEYIQDILQEVWGDRRGELCLDCCCGSGVGTAALRAGGLR
                     AIAYDNDPTLLALGLSKGRLLPSDTMCIDAREADRYITDAPLGIILMAGEIYPYNASL
                     WQDIITTLLDLTDETLITVGTEDEAAMVESWCAGRDTRVFENRRDPLYDRWCCVSRRD
                     "
     gene            complement(8098..8946)
                     /locus_tag="PK196_04515"
     CDS             complement(8098..8946)
                     /locus_tag="PK196_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HON40715.1"
                     /translation="METRVDHGRIRGCNLPDRTELQERALKTEQDIIVGEKCRVDYGL
                     SGKDVVVCEFSRINGSIVAAGDARIDNWCEVNGDVIVDEDAYLGEGVKILGKLVVRGD
                     LDIGDNVQIERGFEAKGWISIRNPMPVIIYIVMYLVAILGIEREDELSSVLEKIFGED
                     ETPAATPLMIPGGSVLDMQTFSVPERMVIGSGCRLHGNIRAASIQVGEGTTIFGSISL
                     REDGSIARGSIVHGDLQSDGDLRIEEGVHILGNVSCRRLTLHEDARIDGVIRAPGGLK
                     IERGPA"
     gene            complement(8963..10201)
                     /locus_tag="PK196_04520"
     CDS             complement(8963..10201)
                     /locus_tag="PK196_04520"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HON40716.1"
                     /translation="MMIAQKVLFLVLDGISDRPCEALDGKTPLAAARTPVLDRIAAEG
                     VCGIMDTISPGIRPGSDTSHLSLLGYPPQDYYTGRGPLEAEGTGIHMTAGMIGFRCNF
                     ATVDENGVVTDRRAGRISGTEPLAEAIRDGVDLSSLGLEFRFNSGAGHRAALALVGEG
                     LGDKVSSNDPKMEGFRPPEIRACTDDPADAKTARACNEFVRQSAEILYHHPINIQRMK
                     EGLAPANYLLIRGAGKMGEFPRFSERYGLSGSVIAAATLIVGIGKVVGLEHIPVPGAT
                     GSVNSDVAAKVRAALAELGRKDFVLMNVKGADEAGHDGNAVQKRNFIEVIDAALEPLL
                     GLDETLIVVCADHSTPCPVRDHSADPVPVVIRGPGVRVDRVRRFDEVSCAEGGLHRIR
                     GSDLMPIVMDLINKSHKYGA"
     gene            complement(10273..10866)
                     /locus_tag="PK196_04525"
     CDS             complement(10273..10866)
                     /locus_tag="PK196_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HON40717.1"
                     /translation="MAKKKQAGRRLEGWKAKKWYRVYVPETFGKVEIGDTISADPENV
                     IGRVMTTTFNEIAQDYSKSHIKMRFKINNVAGDVAYTEFVGHEVTRDYLRSMVKRRTS
                     RIDTILPLLTRDGKLLRVTVVCLTLTRAEQSQVHAIRKAVTQMLNAKAAESDFDTLVK
                     EIVSGDLAREIFKAVKTIYPMRRVEITKSRLEQIAAV"
     gene            complement(10885..11157)
                     /locus_tag="PK196_04530"
     CDS             complement(10885..11157)
                     /locus_tag="PK196_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HON40718.1"
                     /translation="MIRVLGVIETRHNHPGCVAAALEPDNLNLIRTVQIEGGVQALVD
                     GARLRSIIATVDDYLMNLAIADDVCNAAMRQQKGQEVNSGSNGMKN"
     gene            complement(11154..12371)
                     /locus_tag="PK196_04535"
     CDS             complement(11154..12371)
                     /locus_tag="PK196_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HON40719.1"
                     /translation="MSLDAAAATLASRLLEQEFVEVLAHHDADGIAAASILCHAMFRA
                     GGRFRLRIRPDITPEEIPREGSVLLCDFGSALQDLPDDVMVVDHHQPRFEGELHVNPH
                     LAGIDGDRNLSAAGAAYLVARKMGDNRDLAGLTLLGIIGDGQVIEGPNREITNEGIAN
                     GFITPGRGLLLVGRGLVEQIALSIDPYLPGLSGEPDAARTLVAAVTGEDEVDMETLLS
                     RIVLKAAREASVTALCSLWGTTYSLGREVIDGAGDLTAVVDACGKAGKGDIGASLCLR
                     SSHALQEAREITARYRQSVITGVREARRLDERVGLYEVDDENVASDVADVLAYDLVQD
                     GPVFVIGRGSGRCSISARCPPGVDLDLDALMRTVARACGGHGGGHRQRAGARIPAGGL
                     DRFREELLEEILP"
     gene            complement(12379..12837)
                     /locus_tag="PK196_04540"
     CDS             complement(12379..12837)
                     /locus_tag="PK196_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HON40720.1"
                     /translation="MARMYARRRGTSGSVRPYRKEAPEWSNTDAAEIEKIIIELRKDG
                     LSASQIGLVLRDRHAVPDVRLATGKRITEILRENGLESEIPEDLRNLMQKALRLRKHL
                     AENKKDYHNLRQLQIAESKVRRLVNYYVKTGRLPADWTYKPETAEILLTR"
BASE COUNT         2449 a         3996 c         4008 g         2564 t
ORIGIN      
        1 ccgcatgctc ccgccgtaga ccacctgtcc gaaacttatg cacccgtccc ccagcggata
       61 ctcgcggctg acgatgaact caagccccgc agccataacc cccgccccga tcgtctcccg
      121 gatcgcccgg ttgtaggcca ctccgcccga gagcaccacc cgcgggatcc cgcgctcctc
      181 tgccgcccgg actgccatag tggttatccc acgcgccagg ttgaactgga tggacgcggc
      241 gatatcggcc accctctctc ccgcaaccat acgcctgtac gcctcctcca ggagagaccg
      301 ggtcgataga acctcgcacc ccccatcctg ccggaactcg aggtcccaga ccgaagccct
      361 gcccccgtat gcctccgact cgagttccat tgccggttca ccatcgtatg tccgctcccg
      421 gcagatcccg agcagggccg aggcggcgtc caggacgcgg ccggtgctcg atgtcgcggc
      481 gacgttcaac ccccgctcaa cctgctttgc aaggacggcg agctccatat cgctccaccc
      541 ccgcgaagca agcaggccgc ggatctccgc cgtcggcagg atgccgtaga gcatccgttc
      601 cgggaaccgg gtggcgagat cgccgccggg catcgggaca acctccaggt gagcgaccct
      661 ctcaaggtgc ggaacctctc ccgcaaagac ctccccgccc cagacagtgc cgtcatcgcc
      721 gtacccgaca ccgtctatgg cgatccccac acactcctcc cgtgtggcgg cggcgatgtg
      781 cgcccggtgg tgctggacgg caagaagttc cgccccgttt gcttcggcaa cctcccttgc
      841 ataacgggtc gagaggaact gcgggtggag gtcgtggaca accatatcat agcgtgcgtc
      901 ggtgagtttt ccgatcttct ccaccgtctc cgtaaggtag gccagagtct gggggttcct
      961 gacattccct atatggggag acgttatgca gaacccgttt ttgtagaccg atatgttcga
     1021 gttcagttcc gggccgacgc ccaggatgca cgcatcgccg agatctatgg cctcccgctt
     1081 tggggcaaga ccgcgggaga gtcggatgat gcagccgtct ctcacaaccg aatcgtcgca
     1141 gcggttgacg atccggcggt tgtgcgtgag tatgtagtcc acctgccgcg gcagacgttc
     1201 cagggcgctc tcgagatccg ttatcatcgg gtagcccggc aggtttgcgc tcgtcatcac
     1261 cagcagccgg tggtcaaggt gcgcaaagag gagatagtgc agcccggtgt atgggagcat
     1321 gcagccgatg gtatgaagcc ttgagattgt ccggtgcgac tccggatcac gcttctcgag
     1381 caccacgatc ggtctctccc gcgagtgcag gatccgccgg tcgtccggag agacgaccgc
     1441 gatccgctcc acctcttccg gttcagccat aaccgcgagc ggctgctccg tccgccccag
     1501 tcggcttttg agttcttcag ccgcctcctc gatacaggct atgtggaacc ctccgatccc
     1561 gcggatggca acgatcgaac cctcatcgag cagggcggcg gcctccctta tagggtcttc
     1621 tgccgcaacc ggtctcccat cggcccttag aagactcaga cgcggcccgc aggccgcaca
     1681 cgcaatcgtc tgggcgtggt ggcggcgaga ggatgggtcg gagtactcgc tctggcaggc
     1741 ggggcagagc gggaactctg ccatcgatgt ccgttcccgg tcgtacggga cgtcggtgat
     1801 tatgctgtag cgggggccgc agttgacgca ggatgtggcc cagtagccct cgtaccggcc
     1861 gccgggcgcg aatatatcgg cgatacactc gtcacatatg gcaacatcgg cggggatgaa
     1921 tcccgtgaga ctccccctcc cgctctcgag gatggtaaac cccgccgtct cctgtccatc
     1981 gcgcagatta taaacatcta cagaatctat gcgggataag ggtgtacccc gggaaacagc
     2041 ctccagaaac tcctcgaaac gctccccgga cgcatggatc tccacctcgc tccccaggtt
     2101 tttgacagtg cccgtgatcc cgaattcccg ggctttagca tagacaaaag gacgaaaccc
     2161 gactccttga acgatgcccc ggatgatgat tgtgccactt ctgcgcattg ggatttcagc
     2221 aaaaatttat ggagttatac ccccatataa caatagggta tcgcagtgac gggacatatc
     2281 aaaattctta acgatccagt ggaactggtt cctcttctca taacgttcaa taatgcagaa
     2341 tataaaaaga tctacgatct cctaaataag gcatggatga ccgaggagga cctcgcggag
     2401 tacgtgggga agcccacggt tgccgagtgt cttgcgatcc tcaagaaggg taaccttatc
     2461 gaggagcaat ggcggatgcc gaaacccggc gagaaaccca cgaaagagta ccgggcaaca
     2521 tacagcaagt tccgggccag ttttcagtgt tccatgggtg atatcgggga tctgcttcat
     2581 atcgccatat caaacgacga gagtctccgt tccatcgtcg atgcggttca acaggagatc
     2641 atgaccggaa caacctctat cggtgacata tcccgcaaat acggggtcag ccccatcttc
     2701 ataaaggggc tggcaaagag gatccccggt ctcgatgtga gagggcaggg gatggttttg
     2761 cttgaccgaa cccagtgaag acccgctcta cgtcatcctg cgcagcaagc gagaggcaac
     2821 ccggttccag attctggtcg agatcgccga acaccagccg gccatccggc agcaggagat
     2881 cgccggaaaa ctcggggtga ccccccaggc ggtctcagag tacatccggg aactggtgga
     2941 tgaggggctt gtcaccgctc acggccgggg ccggtatgag gtcacgaaga gcggcgtcga
     3001 gtgggtgctc cggcacgccg aagcgctcga gtcgtatgcc cgccacgtca cccgcgacat
     3061 catccagcag gtagcggtct ggacggccat cgctgaggac gatatccgga agggggatac
     3121 tgtcggcgtc ttcatgcgta aaggctggct gtatgcgaca gagagggagc agagcgccat
     3181 gggtgttgcg acgatggacg cccggcccgg cgaagacctc ggtgtcgccc ggttgaacgg
     3241 catcatcgag cataaggagg gggccgtcca cgtctgcaag gtcccgcggg tggaacgtgg
     3301 cgggtcacgc atggtcgatc tggatctact cagggatgtc gtccgggatg ctgaggttgt
     3361 ggccgccgtc gggcttgagt cgtatgtggc ccttaaaaag gcgggtatcg aaccggatat
     3421 gttctttggt gcccgggaag gtgtcatcga ggcggcgttc cacggccttg agtgtgctat
     3481 acttatcgtc gatgaagagt ttaccgattt tctcaagcgg cttgagacgg tagggctcgc
     3541 ctacacgatc catgacctga taccgtcatg aagatcatcg tccagccaca gaaaagcgcg
     3601 tacaaactgc tctttgtcga gggcgaccgc gttgaaggga cgggtttcgt caccctcacc
     3661 gcgactcaga agggtcagcg gccgaagaac ttcaagatgc gccgccgcgg cagcagacac
     3721 ccggcacccg tccctacgcg tgacctgatc acgatgctgc gccgctcctc ggtggccctt
     3781 gcagcggaga gcccggagtt cgagtcgttc ctcgccgacc tccagatccc gtcttcacgt
     3841 ctctatatct gccggttctg ccagctcgag gacaggtttg cccccctaga taagacgacc
     3901 ggggtgcgct acggcgggga gtggatctgc atggagtgcg cgaaacgcga gatgcgccgc
     3961 gaactcggct acatggggag gttcggcggt tctgtgctca tccatctgga gaaactcctt
     4021 gcccaggtcc gggatctcga ccgggtgctg gcatcggttg ggccagagcg gcctgaccgg
     4081 cagcggacgc tctttgacag gcttgaggct cacgagatcg agaagactct gcatatcacc
     4141 gatctctcgc tgcctcccgc gttcgtgaag gccgccggcg tcgattacct gatgcccgtc
     4201 cagcacctgg ccgtccagag cggcctcctc gaggggcagc atctccttgt ggtctcggcc
     4261 acggcgagcg gcaagacctt catcggggag atggccggga tgaagaactt cctggagggc
     4321 cgggggagga tgctcttcct ggtcccgctg gtcgcgcttg cgaaccagaa gtaccagcgg
     4381 ttccgggacc gatacggtca ccttgtccgg gtcgccctcc aggtcggggt gagccgtctc
     4441 aacctcccgg agacgcggcc ggccggggac cgtgacgtca atgctccagt ggtggtcggg
     4501 acatacgagg gtatagacca cctcctcagg acggggaaga ctctgaaaga tatcgggacg
     4561 gtcgtgatcg atgaggtcca gatgctcgag gacgcggagc gcggccaccg tctcgacggg
     4621 ctgatcgcac gtctcaagta tatcgcccct gacgcacagt tcctctacct ctcggcaacg
     4681 atcggggcac ccgggctgct tgcagagaaa ctcggggcga accttgtccg ctacgcggaa
     4741 cggccggttc cgcttgagcg ccacctcata tttatcgagc gggagaagaa gatcgcgttc
     4801 ataaagcaga tggtcgccga ggagtaccgg atgcggtctt cgaagggcta ccgggggcag
     4861 acgatcgtct ttacaaactc ccgggcgcgc tgccacgtca tcgccgacgc cctgggtaag
     4921 atggccgtgc cataccatgc ggggctgagc gcccaggagc gccgggaggt tgaacggcgg
     4981 ttctcaaatg gggaggttgc cgctgtcgtg accacggcgg cgcttgctgc cggtgtggat
     5041 ttcccggcgt cccaggtgat ctttgatgcg cttgcgatgg ggatccagtg gctaaccgtc
     5101 caggagtttc accagatgat gggtcgggcc ggccggccgg acttccacga ccttggccgg
     5161 gtggtcatcc tggcggagcc cggcgggtct tactccagga catccgggaa gaccgaggag
     5221 gaggttgcca tcgccctcct gcggggcgag atggaggagg ttgcgccgga gtatgacctg
     5281 gagcagagca cggaggagtt cgtcgcaaac gccgttgtct gcggcggcga cgagcagcag
     5341 atcatacgga tgaaccgcat gatggtcggg acgctggaac ccgctctccc gacgctcctc
     5401 gaccacggac tggtcaggcg gcggggaggg cgtatagaac tcaccgatat ggcgcgggtg
     5461 atggcagagc acttcatcgg cgctgagcgg ctgatcctgg tcagggacct tgtccggcgg
     5521 atggacgatg cagccgggat cgtggcggaa ctcgagtgcg ccgctgagga ggagcagtaa
     5581 aacagtaatt gatcaaggcg tgctctgatc gggttttgtc aatcacggga agaaggtgtc
     5641 cgggtggagg gccgggagcg tctccgccac ggcgagcccc accacgcggg ggtgattctc
     5701 catcgataca ctgtcggcgg aggagaccac caattcgcgg tctcaacgga gactccctga
     5761 gatcccgatt ccttccctgg cacggtatcc ggggctatca tcacgcactg cccgccccct
     5821 gtgaaggagg cgccgaacgc gccgggcggg gtggtcgacg aacaccgttc aacgagagac
     5881 gaaagagaca ggggcgggtg atgccccatc ccggcaccga tcaaggcttt taccgggggt
     5941 cgtcatccgg ggccggcccc gccaccatcc cggcgttctg tccaccgttt atctcgcctg
     6001 ctgccttctg catcataacc gcgaagaccg ctgtcccgag gaggcttata gcgccggctg
     6061 cgtagaatac ggcgtctata ccgatgtagt ccatgaagag accccccatc aacggggcta
     6121 tgatcgtgcc tatcccgaag atggtgttgt aggcgcccat cgacgtcccc atacccagcg
     6181 tccggccggc atcgaccgtc agcgccatga ttgacggctg ctggatggcg ccgccgacac
     6241 cgatcagcga ggttatcacg agcaggtccc agaacgatct cgagaacggg attatggcaa
     6301 gtgcgatcgc tgctatggcc gatcccacga cgatcagggc gactttgttc cccctgtcgg
     6361 tgaactttcc taccggcacc tggaggagcg ccatgatgaa ggtgttcact gagaggatga
     6421 cccccacact ggacgggctg atcgcgatca gcggggcaaa gacggggatg aagaccatcg
     6481 tccctccacg gccgagagcg ctgatcaggg tgaagaccat gacaccccgc atgaccggca
     6541 gcctgaagat atccctcatc ggggcgggct ccccgccctt gatcgcggat gaagcacgtt
     6601 tcgcttcggg gagtgagaca gcgaccagga gtgtggcaaa cgccgagagt gcggccatgg
     6661 tgtagaatac cgagttcatc ccgaaagcgt cgttgaggaa accaccgagg agcggcccta
     6721 cgcccatgcc gaggaacatc gatatcgaga agttccccat ataactccct tcctttccat
     6781 gctccgagag gtcggcgatg taggccattg ctatcgggac gaccatcgct gaggcgatac
     6841 cgtgggcgaa ccggacaacc gtgagtgagt agacgctgtc ggcgatgatg taggcgaggg
     6901 agaggacggc atacgcaaac atgcctatta ggatgatcca cttcctcccc tggcggtcgg
     6961 acaccctccc gatgatcggc atgaagatcg acctggagag cgcgaaggct gaaaagatta
     7021 tcccgagcca gataccggtc gcacccaggt tctccgcgta tagggggaga agcgggctga
     7081 caacaccaag cccgagcatc gtggcaaaga cggagatgaa taagacatta tatatgcgtc
     7141 tggctgtttt catgcagatt acacctgata tatttaaaaa accgcagcca ctctcccgcg
     7201 gctaaagaat tacaattgca gtaagaatgg ttggtggctt tgcatcatat acgtttagat
     7261 cgttgcaggt tcagtcgcgg cgtgaaacgc agcaccacct gtcgtataac gggtcacgcc
     7321 ggttctcaaa gaccctggta tcccgtccgg cgcaccacga ctccaccatg gccgcctcat
     7381 cctccgtgcc gacggtgatc agcgtctcgt cggtgaggtc aaggagggtg gttatgatat
     7441 cctgccagag gctggcgttg taggggtaga tctcccctgc catgaggatg atcccgagag
     7501 gagcgtctgt aatgtagcgg tcagcctccc gtgcatcgat acacatggta tcggatggca
     7561 ggagcctacc tttcgagagg ccgagcgcca ggagcgttgg atcgttgtcg taggcgatcg
     7621 cgcgcagacc gcccgcacgg agcgctgcgg tgccgacccc ggagccgcag cagcagtcga
     7681 gacagagttc ccccctccgg tcgccccaga cctcctggag gatgtcctgg atatactctg
     7741 cccggcccgg gcgcaggtcc tccagggcgg gtgatgttgt ctccaggagt tccatgctgt
     7801 agtagtcgcg gacggctctc gcccaggtct cccgtctctc ctggtagata tccccgccga
     7861 tcgcctcgaa gtagtctatg acggcaaggt tcgggtcgcg gtagaggaac gaggcgacgt
     7921 gccagcctct cccgtcgtgg agcgcaagcg cgatcacctc ctcatcctca tcgaccagga
     7981 ggcgagcctc gtccgcccga acctggaaga ggtaatctgt gcaggaatcc agcgtcagat
     8041 ccgcaaaaga gggttcgatg agcctgcagt cctcgacctc aagaatatct gcaaccctca
     8101 tgccggcccc ctctcgatct tcaatccgcc cggcgcccgg atgacaccat cgatgcgggc
     8161 gtcctcgtgc agcgtgaggc ggcggcagga gacgtttcca aggatgtgca caccctcttc
     8221 tatcctcaga tcaccgtcgc tctggagatc cccatggacg atcgaacccc tagcgatgct
     8281 cccgtcctcc cgaaggctga tacttccaaa gatcgttgtc ccctccccta cctggatcga
     8341 agccgcacgg atgttcccgt gcagccggca gccagagccg atgaccatcc tctccgggac
     8401 ggagaaggtc tgcatatcca ggactgaacc gcccgggatc ataagcggcg tggcggcagg
     8461 agtctcgtcc tcgccgaaga tcttttcgag gactgaactc agttcatcct ccctctcgat
     8521 gccgagtatg gccaccaggt acatcacgat gtagattatg accggcatgg ggttgcggat
     8581 ggagatccag cctttcgcct caaacccgcg ctctatctgg acgttgtcgc ctatgtccag
     8641 gtctccacgg acgaccagtt tgccaaggat cttcacccct tccccaaggt aagcgtcctc
     8701 atccacgata acgtcgccgt tgacctcgca ccagttatcg atcctggcat cgcctgccgc
     8761 aacgatgctg ccgttgatcc tgctgaactc acagacgacg acgtcctttc ccgagagacc
     8821 gtaatcaacc cggcacttct cgccgacgat gatatcctgt tcggtcttga gcgccctctc
     8881 ctggagttcg gtgcggtccg ggaggttgca gcccctgatc cggccgtggt ctaccctggt
     8941 ttccatttac tctgtatgat cttcatgcgc catacttatg actcttattg attaggtcca
     9001 tgacgatcgg catgaggtca gagccgcgga tacggtgcag tcccccctcc gcacacgaga
     9061 cctcgtcaaa tcgtctgacc cggtcaaccc tgactccggg gccacgtatg acgaccggca
     9121 ccgggtcggc gctatgatcc ctgaccgggc agggcgtgct gtggtcggcg cagaccacga
     9181 tcagggtctc gtcgagaccg agcagcggtt cgagcgccgc atcgataacc tctatgaaat
     9241 tccgtttctg gacggcgttc ccatcgtggc cggcctcgtc tgcgcccttg acgttcatca
     9301 ggacgaagtc tttccggccg agctccgcca gcgccgcccg caccttggcg gccacatcgg
     9361 agttgaccga ccctgtcgcc cccggaaccg ggatgtgttc aagccccacg acctttccga
     9421 ttccaacgat cagcgtggcc gccgcgatca cgctcccgga aagcccgtaa cgctctgaga
     9481 accgcggaaa ctcgcccatc ttcccggcac cgcggatcag gaggtagttt gcgggcgcca
     9541 gcccctcctt catccgctgg atgttgatgg ggtggtggta gaggatctcc gccgactggc
     9601 ggacgaactc gttgcaggcg cgggcggtct ttgcgtcggc ggggtcgtcg gtgcaggccc
     9661 ggatctccgg cgggcggaaa ccctccatct tcgggtcgtt ggaggagacc ttatccccga
     9721 gcccctcccc gacgagggcc agggcggccc ggtggccggc gcccgagttg aacctgaact
     9781 ctagaccgag cgatgagagg tcgacgccat cccggatcgc ctccgccagg ggttccgttc
     9841 cggagatcct ccctgcccgg cggtcggtga ccacaccgtt ctcgtcaacg gtggcgaagt
     9901 tgcagcggaa cccgatcata ccggcggtca tgtggatccc ggttccctcg gcttcgagcg
     9961 gcccgcggcc ggtgtagtag tcctgcggcg gatagccgag cagtgacagg tgggatgtgt
    10021 cggatccggg gcgtataccg ggggatatgg tgtccatgat cccgcatacg ccttcagcgg
    10081 cgatccggtc gagaacgggt gttcgtgcag cggcaagagg cgttttcccg tccagcgctt
    10141 cacatgggcg gtctgagatc ccgtccagta ccaggaatag aaccttctgg gcaatcatca
    10201 tgaagagatg atgatctaaa acctgattac tcttcccggt ggagcgggtg aaatgaggtt
    10261 gagcagtggg ccttatacgg ccgcaatctg ctcaagtctg gactttgtga tctcgacccg
    10321 gcgcataggg tagatcgtct tgacggcctt gaagatctcc cgagcgaggt cgccggagac
    10381 gatctccttg accagggtat caaagtcgct ctccgctgcc tttgcgttga gcatctgggt
    10441 taccgccttg cggatggcat ggacctgact ctgttcagcc cgcgttagtg ttaggcagac
    10501 gactgtcacc cggaggagtt tgccgtccct ggtgagaagc gggaggatgg tatcgatacg
    10561 ggacgtccgc cgcttcacca tcgaccgcag gtagtcgcgg gtcacctcgt gcccgacgaa
    10621 ctcggtgtag gcgacgtctc cggccacgtt gttgatcttg aacctcatct tgatgtgcga
    10681 ttttgagtag tcctgtgcga tctcgttgaa ggtggtggtc atgacgcgac cgatgacgtt
    10741 ctcggggtcg gccgagatgg tgtcgccgat ctcgaccttg ccgaaggtct cgggtacgta
    10801 gacacggtac cacttcttgg ctttccagcc ttcaagcctt cttccagcct gtttcttctt
    10861 tgccatggta tcacttcatc aatatcaatt tttcatccca tttgagcctg aattcacttc
    10921 ctgacccttt tgctgccgca tggcggcgtt gcagacgtca tctgctattg ccaggttcat
    10981 gaggtagtcg tccaccgtcg ctatgatcga ccggagtctg gctccatcca ccagcgcctg
    11041 cacacctcct tcgatctgaa ccgtcctgat gaggttgagg ttgtcgggct cgagcgccgc
    11101 ggccacacac ccggggtggt tgtgccgggt ctcgatgacg cccaaaacac ggatcatggg
    11161 aggatctcct ccaggagttc ctccctgaaa cggtccagcc cgccggcggg gatccgtgcc
    11221 ccagccctct ggcggtggcc gccgccatgg ccgccgcagg cccgggcaac cgtccgcatg
    11281 agcgcgtcca ggtcgaggtc gacgcccggc gggcagcgtg ctgagattga gcagcggccg
    11341 ctgcccctgc cgatgacaaa cacggggccg tcctggacca ggtcgtaggc gagcacatcc
    11401 gcgacgtcgc ttgcgacgtt ctcgtcgtcc acctcataga ggccgacgcg ttcgtccagg
    11461 cggcgcgctt cgcggacgcc cgtgatgacc gactgcctgt agcgggcggt gatctcccga
    11521 gcttcctgga gtgcgtggct cgagcggagg cagagcgatg cacctatatc acctttccca
    11581 gccttgccgc aggcgtcaac gacggcggtg aggtcacctg cgccgtcgat cacctcgcgg
    11641 ccaaggctgt aggttgtccc ccagaggctg cagagggcgg tgactgacgc ctccctggcg
    11701 gcttttagga cgatccggga gaggagcgtc tccatatcaa cttcgtcctc accggtgacc
    11761 gctgcgacca gtgtgcgggc ggcatcgggt tctccggaga gtccagggag gtaggggtcg
    11821 atcgagagtg cgatctgttc gacgagccct ctcccgacaa ggaggagacc gcggcctggc
    11881 gtgatgaacc cgtttgcgat cccttcgttt gtgatctccc gatttgggcc ctcgataacc
    11941 tggccgtcgc cgatgatccc aaggagcgtg agccccgcga gatcccggtt gtctcccatc
    12001 ttcctggcga caaggtatgc ggcgccggcc gctgagaggt tccggtcacc gtcgatcccg
    12061 gcgaggtgcg ggttgacgtg gagttcgccc tcgaaacggg gctggtggtg gtcgaccacc
    12121 atcacgtcgt ctggcaggtc ctgaagtgcc gatccaaagt cgcatagcag cacgctgccc
    12181 tcccgtggga tctcttccgg cgtgatgtcg gggcggatcc tcaggcggaa ccggccgccc
    12241 gcgcggaaca tggcgtgaca gaggatggat gcggcggcta tgccgtcggc atcgtggtgc
    12301 gccagcacct ccacgaactc ctgttcgagc aggcggctgg ccagggttgc agcagcggcg
    12361 tcaagcgaca tgggtcgatc agcgtgtaag caggatctct gcggtctccg gtttgtatgt
    12421 ccagtcggcc ggcagccgtc cggttttaac gtagtagttt accagtctgc ggaccttgga
    12481 ctcggcgatc tgaagctgcc tgaggttgtg gtagtccttc ttgttctccg cgaggtgttt
    12541 cctgagcctg agcgctttct gcatcaggtt ccggaggtcc tcggggatct ccgattcaag
    12601 accgttttca cggagaatct ctgttatacg tttgcctgtg gcgagcctga cgtcagggac
    12661 ggcgtgcctg tcgcgcagga caagcccgat ctggctggcc gagaggccgt ctttgcggag
    12721 ttcgatgatg atcttctcga tctccgctgc atccgtgttt gaccactcgg gcgcctcttt
    12781 acgatagggg cggactgaac cgctggttcc ccggcgccga gcgtacattc ttgccattca
    12841 tgcacctccg ttaggtatta actatgtaag tccagaaaag agtccgtttc ctgactattc
    12901 tctgtaatcc cgagcccttg cgggcagtgc ttccgggagc acgtcggact taccatagcc
    12961 agcacataga gtgctctact ttatttaccg ggaagaatct taagagtatc ggactct
//
LOCUS       DAOSZX010000037        12984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4045_length_12984_cov_21.752804, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000037 DAOSZX010000000
VERSION     DAOSZX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4045_length_12984_cov_21.752804"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1020
                     /locus_tag="PK196_04545"
     CDS             235..1020
                     /locus_tag="PK196_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON40721.1"
                     /translation="MKLTILASGSKGNCACLQGERGALLIDAGLSARETLRRLDAAGG
                     DASLIEGILVTHEHIDHVRGLDVLSRRLGVPVYATGGTLEAILEKSEASMADLRRCRS
                     GERFSLGDFSIEPFPTSHDAREPCGFCIAEGDLRIGCCTDTGTITTTISDRLATCDAV
                     LLESNHCPDMLENGPYPPYLKNRIRSKRGHLSNPDAARCLQRLADHIQVAMLAHLSEV
                     NNTPEKVRLSALEGLGLYSDQVGVGVAPQDPGAVHPESCGFRL"
     gene            complement(1028..2314)
                     /locus_tag="PK196_04550"
     CDS             complement(1028..2314)
                     /locus_tag="PK196_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON40722.1"
                     /translation="MREERGENDTVEAPSTRSDGDESPEPGRELCLSILRKAPDGFVL
                     LDGKGRYRYMNPAFTRITGYTLEDLPDISTWFECAYPNPTYRQKVHELRQELFSGRCN
                     SMVVTIVRKDGRVREIEARRTPIDDDCILLSVRDVTEQTLIEESLQQTASELSAVIDS
                     FPDLFLRLNADGTILDTKAGRLVERPMITRTHLGRRVQDLLPPGASEAFLGALREAVR
                     TNARTAPIEFECRAEGETRCYEARVVPLHDTHLMAIIRDITERKRAEEELQRYREHLE
                     EVVAERTAELRRANEHLKHLVYCIEVTERKAAERWLDRSAEKGTIAVDEPESVRITTD
                     ETGTIIIADLTAERLSGFSRDELVGKTVWSLFSGGGVRETLSREVLEGGRSAECSEGV
                     ALVRKDGSKEAVHISAEPIKSAAGAVIGMICTLRKG"
     gene            complement(2548..3723)
                     /locus_tag="PK196_04555"
     CDS             complement(2548..3723)
                     /locus_tag="PK196_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40723.1"
                     /translation="MERAWQPEALQNRRLEQLRATMDDYIAKNIEQIEGNLPVFFGHV
                     AATLDRSFPDLDDRTYDDFIDETGFALLNAKRGPPTPEFLEKVLRHAVGNKKEQKGRA
                     TLEVMVALNLIDIGSYYQALEYLSRHRNHDVRINAAIAYCYYALSLAKNQSPGPRPDD
                     MELQAREEMLTLALVRPPLSRLGPLDRKESRLNSVFWYMLDLAFTWFPSEPAFYRIGI
                     AWTKHVGDAERRLQLLDRATEHFANDRYFLAEAFNTSVDLRNGSGAASIVKQMMQQFP
                     DDHEPLYYGMKLAILGGKPGSYARFRKLAILKEFPRRLLLLLDTVLEVMCNHRTESLL
                     CFEEVKKASMARDYYLTALEYILRDFVEGDEERAKRARSTFFSSVDQYCMQTLKIRI"
     gene            3946..4356
                     /locus_tag="PK196_04560"
     CDS             3946..4356
                     /locus_tag="PK196_04560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HON40724.1"
                     /translation="MGYIERLKEVGRDANPFFKMMGIEVEDFGDGYASLWMEVRPDML
                     NGAGWLQGGVYVALADEAVALALYTLLEEGERIATIDEHTCFIKGVNSGVIAATGDVI
                     RKGRRVAFADGEVTGGEDGALLSRTSTSYAVIGR"
     gene            4475..6085
                     /locus_tag="PK196_04565"
     CDS             4475..6085
                     /locus_tag="PK196_04565"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HON40725.1"
                     /translation="MKYIVVTGGVMSGLGKGITTASIGRILKNRGYEVTAVKIDPYLN
                     IDAGTMNPAQHGEVFVLSDGGEVDLDLGNYERFLDINLKSIHNITTGKVYRNVIEKER
                     RGDFLGATVQIIPHITDEIKQSIRRAAEEPVDGGRKAEICLVEVGGTVGDIESMPFLE
                     AVRQMHGELAREDMILVHVTLVPVDTMGDHKTKPTQHSVKALRELGLQPDIIVGRSRE
                     VLSQQTKKKISAFTDVPAKAVISAKDVPDIYQIPMELEKEGLADVVCSYLGLENRQPD
                     PAWYRVVSMEYTNRVTVAIVSKYGIEDVYMSIKEALKHAGRALSTEVNIRWLDAEAFD
                     PRSLADIDGILVPGGFGKRGIEGKIQAIRYARENKKPYLGLCLGFQLAVIEYARHILG
                     IEDATSEEIGEGTHVIALLPEQEGISELGGTMRLGDCSVTLKEGTMVAGLYGETSIVE
                     RHRHRYEVNPAFIADIEAAGLVFSGFCGQRMEVCELPGHPFYLATQFHPEFRSSPTRP
                     SPPYVGFVEACKKNKSTQSQVAEQNDER"
     gene            6072..6989
                     /gene="guaA"
                     /locus_tag="PK196_04570"
     CDS             6072..6989
                     /gene="guaA"
                     /locus_tag="PK196_04570"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HON40726.1"
                     /translation="MMNVEKFIDQAVSGIRDAAGGEKVVMALSGGVDSSVCAALGARA
                     IGDRLIPIYVDTGLMRKGETERIRSLFADKNLRVVDAADEFFEALAGVTDPEGKRKAI
                     GEKFIRVFEREAKRTGATMLLQGTIYPDRIESEGGIKSHHNVGGMPLNIDFKGVIEPL
                     ADLYKDEVREVAAALGLPAEIQHRMPFPGPGLAVRVLGEVTREKIEIVREANAIVEEC
                     LVEEFGPWQCFAALIGLGTGVKGDVRLHGWIVAVRAVQSRDGMTADPLRLPYETLFRI
                     MARITAEIPGVSRVVYDVTPKPPATIEYE"
     gene            7000..8124
                     /locus_tag="PK196_04575"
     CDS             7000..8124
                     /locus_tag="PK196_04575"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HON40727.1"
                     /translation="MEQDSRELDARYYMPAFSRTIKIVRGEGSRVWDDRGREYIDCVA
                     GIAVCSTGHCHPKVVRAICDQARQLIHCSNLYYVPNQAELAERLVEITGLAKAFFSNS
                     GAEANDGAIKLARIRTGRKKFVAFTHGFHGRTCGSLAITHKPAIREPFEPLSPPCTFV
                     DYGDLDALAEVADADTAGVFVEPIQGEAGVLIPPDGFLQGIREICDDSGALMIVDEVQ
                     TGMGRTGRWFAFQHTGVTPDIVTIAKGLASGFPIGALVAREGLEFRKGEHGSTFAGGP
                     VACAAALATIGIIKGLLPDIPRKGELFMKGLAAHNPRGRGLMIGISVGDRCTEVQQTC
                     ADNGVLVNCAADGNLRLIPPLVITDEEIETAVGVIDAALG"
     gene            8108..9166
                     /gene="hisC"
                     /locus_tag="PK196_04580"
     CDS             8108..9166
                     /gene="hisC"
                     /locus_tag="PK196_04580"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HON40728.1"
                     /translation="MQRLVRACFSGASGYSFAKKAADVAREHGIERVARLASNENPRP
                     PSQAVIEAGRAALLEANRYPDERASVFVEALRRYHGDYRFVTGAGMDGVIETLIRTVV
                     EPGETVVISTPTFSFYGIAAIAHGARVVNAPRRKEDFSVDPADFIRACGGAKLAFLCT
                     PNNPTGNTAPLETVEEILEGLEGLLFLDNAYIEFSDMDYLPLMHRHENLVIGRTMSKI
                     FALAGLRVGYAFVPGWLEPFYNRAATPFALNSVSAAAAAAALAEEERVRETREYVLCW
                     RRRFIEEIPGAYPSDANFVMIDVAPYTGDEVVERLAARGVLVRSCTTFPGLGDHYIRV
                     SIGDDRENALFMEAIRDL"
     gene            9163..9675
                     /locus_tag="PK196_04585"
     CDS             9163..9675
                     /locus_tag="PK196_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HON40729.1"
                     /translation="MMVGLTGTPGTGKSSVAAELERRGHRVVHLVDTVQPYILEEDRA
                     RQTMVVDTDRWVAEFEPVDGIIEGHLAHLLPCDLVVVLRCRPDVLRRRLELRGYPRAK
                     IAENVEAEALDVILIETLDEHPAEHVLEVDTTDLSIEECAGLVERFISGELPPSFGSI
                     DWTDYLETGL"
     gene            9672..10274
                     /locus_tag="PK196_04590"
     CDS             9672..10274
                     /locus_tag="PK196_04590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HON40730.1"
                     /translation="MTLDQYRPQAQRIMQPAINLVRRAGITPDALTVGSFIVSALAGI
                     AFYLGGVVSGTILVALNAAFDALDGALAREMGIAGAHGDFLDHVIDRYADIFIITGIF
                     AGGAAPWQIGVFALTGVLMSSYLGTQAQAVGVGRFYGGILGRADRLLLIIIAGALTVL
                     VPVEICGLNYLGWLLVIFGILGHYTAIQRFIHVWREIGKK"
     gene            complement(10284..10775)
                     /locus_tag="PK196_04595"
     CDS             complement(10284..10775)
                     /locus_tag="PK196_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HON40731.1"
                     /translation="MKPAPVYIRAVQAIVAIGICLAAAKIGSAFAMPAIPTWYASLIK
                     PELTPPAWVFAPVWTVLYVFMGIALYLVWSKGWKQRGVPVAVGIFGVQLFLNVLWSYA
                     FFGLQAPFLALTVILLLWIAILLAIVAFYRVSIPAAALLVPYLIWVAFAAYLNHGIYI
                     LNP"
     gene            complement(10952..11212)
                     /locus_tag="PK196_04600"
     CDS             complement(10952..11212)
                     /locus_tag="PK196_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HON40732.1"
                     /translation="MKPEVLLSGSSFKEAREFIRKNVKEYYEVEPGYRILDVHIIGVP
                     PLYVGIEGENIVFPYTKPCHGTFVVKIPGSDEIARLRERKRK"
     gene            complement(11209..11691)
                     /locus_tag="PK196_04605"
     CDS             complement(11209..11691)
                     /locus_tag="PK196_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HON40733.1"
                     /translation="MVETGNLKNEALLLLGCPQVPVQTSLALYAAHNLRRQGCRPVIA
                     GTPAARKLMEVADPGRCYIDEMNDLDNMIDEIAEKQRDFKLCFVFIHNDSGVAYAGTM
                     AYISKARVYALIFGEHAEELAGEIEFPCEIIAAKAVHNPMPLKRRLDEVMQWPVVSKL
                     "
     gene            complement(11685..12431)
                     /gene="fdhD"
                     /locus_tag="PK196_04610"
     CDS             complement(11685..12431)
                     /gene="fdhD"
                     /locus_tag="PK196_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HON40734.1"
                     /translation="METVRRTGIQVNGDEVCEVQDSIIVEGRVRLFLNGEYLTTIVAS
                     PERLEDLGAGFVVCEGLAETVESVEVSGGDVHIAAPVKREFLLEMESSGGYRVLGEAK
                     EVDSTITITADGVRAVTAAIESDVWRRTGGVHCSVLFCDGDLVTRACDVGRHNTVDKV
                     VGYATLRGLDRSGCVLGCTGRQPAGMVAKAANAGIPIVVSRAASTDRGILTAERAGIT
                     LIGFSRGERFTIYTHPERVPEVLKKLGRSW"
     gene            complement(12431..12748)
                     /gene="cutA"
                     /locus_tag="PK196_04615"
     CDS             complement(12431..12748)
                     /gene="cutA"
                     /locus_tag="PK196_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HON40735.1"
                     /translation="MDPPDCIMVFCTAPAGEAENLARALVDARLAACVNVVDVRSCFW
                     WEGAVSTEDERLLIMKTQHRLLDPLIERIRGQHTYDLPEIIAIPIIGGYGPYLNWIRE
                     ETG"
     gene            12788..>12984
                     /locus_tag="PK196_04620"
     CDS             12788..>12984
                     /locus_tag="PK196_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40736.1"
                     /translation="MKGGRSGKSRIFEPGMKTEPPFFLVGRVGWGKELFPRLRISSGI
                     RPFIDPPSLPISRELRLAKPWL"
BASE COUNT         2588 a         3767 c         3906 g         2723 t
ORIGIN      
        1 gacaactgga gcagccctgc tggatgccac caccttgatc ctgacttaac ctttttttcc
       61 atgaaaacca ggcctccgag atccagatgg tgtaaagcgg tctgcggcga tacagatctc
      121 ccatggatcc gttcccggaa aggttcatca gatcgggccc tccggccttg aggaacggtt
      181 ttgatggcct caaatacctt tatcccctat ctccagagaa tcttaacaga actgatgaaa
      241 ctgacgatcc ttgccagcgg gagcaaaggc aactgtgcct gccttcaggg ggagcggggt
      301 gcgctgctca tcgacgccgg tctatccgcc cgagagacgc tccggcgcct tgatgccgcc
      361 gggggggacg cctcgctcat cgagggcata ctcgtcacgc acgagcacat cgaccacgtt
      421 cgcggtctcg acgtcctctc aaggaggctc ggtgttccgg tctatgcaac cggcggcacc
      481 ctggaggcta tcctggagaa gtccgaagcg tccatggcgg atctccggcg gtgccgttcc
      541 ggcgagaggt tctccctcgg cgacttctcg atcgagccct tcccgacttc tcacgatgcc
      601 agggaaccct gtggattctg tatcgccgaa ggggatctcc gtatcgggtg ctgcaccgat
      661 accggcacca tcaccaccac aatctctgat cgactcgcca cctgcgacgc tgttctgctt
      721 gagagcaatc actgccccga tatgcttgag aacggtccat accctccata cttgaagaac
      781 cggataagat cgaagagagg ccatctctca aaccctgatg ccgcccggtg cctccagcgt
      841 ctggccgatc atatccaggt ggcgatgctt gcccatctta gcgaggtcaa caacaccccg
      901 gagaaggtac gtctctcagc gctcgaaggg ctcgggctct actctgacca ggtgggtgtc
      961 ggcgtggcgc cccaggaccc cggagcggtg catccggagt cctgcgggtt caggctctga
     1021 cgtccgctca gcccttccgc agtgtgcaga tcatgccgat cacagcacca gcggcactct
     1081 tgatgggttc ggcggagata tgaaccgcct ctttcgaccc gtcttttctt acgagcgcca
     1141 caccttctga gcattcagcg gatctgccgc cctccagcac ctcacggctc agggtctctc
     1201 tcacaccacc gccggaaaag agcgaccaga ccgtttttcc gacaagttca tccctgctga
     1261 aaccagagag gcgttctgct gtcaggtcgg cgatgatgat cgtcccggtc tcgtcggtgg
     1321 tgatcctcac actctcgggt tcatccaccg cgatagttcc cttctcagcc gatcgatcca
     1381 gccatctctc tgcggctttg cgctcggtca cctctatgca gtagacgagg tgcttgaggt
     1441 gctcattcgc ccgcctgagt tcggcggttc gctcggcaac aacctcctcc aggtgctccc
     1501 ggtaacgctg cagctcctct tcggctctct ttctctctgt tatatccctg attatggcca
     1561 tcaggtgcgt gtcatggagc ggcacaaccc tggcttcgta gcagcgcgtc tccccctccg
     1621 ccctgcactc gaactcgata ggcgcggtgc gggcgttggt cctgacggcc tctcgcagtg
     1681 cacccaggaa cgcctcgctt gctcctgggg gcagcaggtc ctgcacccgt cgccccaggt
     1741 gcgtccgggt gatcatcggc ctctccacca gtcggccggc ctttgtgtcc aggatcgtgc
     1801 cgtcagcgtt cagcctgagg aagagatccg ggaaagaatc gatcaccgcg ctcagttcgg
     1861 acgcggtctg ctggaggctc tcctcgatca gggtctgctc ggtgacgtcc cttacggaga
     1921 ggaggataca gtcgtcatcg atgggtgttc ggcgggcctc gatctcgcgc accctgccgt
     1981 ctttccggac aatggtaaca accatggagt tgcacctgcc gctgaagagc tcctgcctga
     2041 gttcatgcac cttctgcctg taggtcgggt tcgggtaggc gcactcaaac caggtgctga
     2101 tatcggggag atcctccagg gtataccctg tgatcctggt aaaagccggg ttcatgtagc
     2161 ggtacctgcc tttcccatcc agaagaacga acccatccgg ggctttgcgg aggatcgaga
     2221 ggcagagttc tcttcccggc tctggcgatt catcgccatc gcttctcgtg ctgggcgcct
     2281 ccacggtatc gttctctcct ctctcctctc tcatcgtaac cagacttcca ttggacgcct
     2341 ccatattcgg atctgcaggc acgccttgca ggatgttctt ctctcaggag taaatatatc
     2401 catcgttacg cctttctgct caacgtttga ggatcgtcgc ttcagcaggc catcgtgcaa
     2461 cccggctata aagtccgccg gggcacgccc tctccatcca cctgtcatct ttctgccccg
     2521 cgaaagactg atcataacac cacatcatta tatcctgatc ttgagtgtct gcatacaata
     2581 ctggtcaacg cttgagaaaa acgtagatct ggcgcgtttt gcacgttctt catccccttc
     2641 aacgaagtcc cggaggatat attccagcgc cgtcaggtag taatcgcgcg ccatcgaagc
     2701 cttcttcacc tcctcaaagc agagaagact ttcggtcctg tgattgcaca taacctcgag
     2761 cacggtgtca agcagcagga gcaggcgacg gggaaactct ttcaggattg ccagtttccg
     2821 aaaccttgca taagaacctg gttttccacc aaggattgcc agcttcatcc cataatagag
     2881 cggctcgtgg tcgtctggaa actgctgcat catctgtttg actatggatg cggcaccgct
     2941 cccgttgcgc aggtccacac tggtgttgaa tgcctcagcc aggaagtacc tgtcgtttgc
     3001 aaaatgttcg gtggcacggt ccaggagttg cagtcggcgt tcagcatctc ccacatgttt
     3061 tgtccatgcg atgccgatgc ggtagaacgc cggttcgctt gggaaccagg tgaacgccag
     3121 gtcaagcatg taccagaata ccgagttcag ccggctctcc ttacgatcga gcggcccaag
     3181 acggctgagc gggggacgaa caagggcaag agtcagcatc tcctcgcgag cctgaagttc
     3241 catgtcgtcg gggcggggac caggactctg gttctttgcc agggagagcg cgtagtagca
     3301 gtaggctatt gcggcgttga tgcggacgtc atggttcctg tggcgtgaaa ggtattcgag
     3361 tgcctgatag taactgccga tatcgatcag gttgagggcc accatcacct caagcgttgc
     3421 ccgccccttc tgctcttttt tgttcccaac ggcatgccgc agcaccttct ccaggaactc
     3481 gggtgtcggg gggccgcgct ttgcgttaag aagtgcaaaa cctgtttcat caatgaaatc
     3541 gtcgtaggtt ctgtcatcga ggtcggggaa cgaccgatcg agcgtggcgg cgacatgccc
     3601 gaagaagacg gggaggtttc cttcaatctg ctcgatgttc ttcgctatat aatcgtccat
     3661 ggtagccctg agttgttcga gtctcctgtt ctggagagcc tctggctgcc acgctctttc
     3721 catggatcta ctggtggaag gtggaagaga tatttgttgt catatgctta cctggctaaa
     3781 acgcaggaat tttgaggctt gatcagcctt tcatcgcata aatgcctgta gacactcagc
     3841 aggacagaat aacgccaatc tgcgtgaccc cttctccacc ggcacccgta ttcataatgc
     3901 ttatcattgg taatcccaaa ccctcaagaa ggcgggtatg atagcatggg ctacattgag
     3961 aggttaaaag aggttggaag ggatgcgaat cccttcttca agatgatggg tatagaggtt
     4021 gaggattttg gagatgggta tgcctctctc tggatggagg tcagaccgga tatgctgaac
     4081 ggtgccgggt ggctccaggg tggtgtctac gtggcgcttg cggatgaggc ggtcgcgctt
     4141 gccctctaca ccctccttga ggagggagag cgtatagcca cgatcgacga gcatacctgt
     4201 ttcataaagg gcgtcaattc cggtgtgatc gccgccaccg gggatgtcat ccgcaaaggc
     4261 cgccgggtgg cgtttgcaga tggcgaggta acgggcgggg aggacggggc gctcctgtcc
     4321 cggacatcga catcttatgc ggttatcggg aggtagcgcc gccctccctt cagagagagg
     4381 ggcgcgcggg atctcccgag caatagattc tcaatacgcg ggatcacata tctaggagag
     4441 aagatctgga aacgatcggg ggaggagcga gagtttgaag tatatcgttg taaccggcgg
     4501 cgtcatgagc gggctcggga aaggtataac cacggcgtcc atcggccgca tcttgaagaa
     4561 tcgtggttac gaagtgacgg cggtgaagat tgacccatac ctgaacatcg atgccggcac
     4621 gatgaaccct gcccagcacg gcgaggtctt cgtcctctcc gacggcgggg aggtagatct
     4681 cgacctcggc aactatgaac ggttcctgga catcaacctg aaatcgatcc acaacataac
     4741 gacgggcaag gtctaccgaa acgtcatcga gaaggagcgg cggggcgact tcctcggcgc
     4801 gaccgtccag ataatccctc acatcacaga cgagatcaag cagagcatca ggcgtgcggc
     4861 cgaggagcct gtcgacggcg gcagaaaggc tgagatctgc ctggttgagg tcggcggcac
     4921 tgtgggcgac atcgagagca tgccgttttt agaggccgta cggcagatgc acggtgagct
     4981 tgcccgggag gacatgatcc tggtccacgt caccctggtc ccggtcgaca ccatgggtga
     5041 ccacaagacc aaacccacac agcactcggt caaggcgctc cgggaactgg ggctgcagcc
     5101 cgacatcatc gttgggagga gcagagaggt gctcagccaa cagacaaaga agaagatatc
     5161 ggcctttacc gacgtcccgg caaaggccgt gatctccgca aaggacgtcc ctgacatcta
     5221 ccagatcccg atggaacttg agaaagaagg gcttgcggat gtcgtctgca gctaccttgg
     5281 actggagaac aggcagccgg atccggcctg gtacagggtt gtctctatgg agtacaccaa
     5341 ccgggtgacg gtcgccatag taagcaagta cgggatcgag gacgtctaca tgtccatcaa
     5401 ggaggcgctc aagcacgctg gaagagctct ctccacagag gtgaacatcc gctggctgga
     5461 tgcggaggcg ttcgaccccc gttctctcgc tgatatcgat ggcatactgg ttcccggagg
     5521 ttttgggaag cgcggtatcg agggcaagat ccaggcgatc cgctacgccc gcgagaacaa
     5581 aaaaccctac cttggtctct gcctcggttt ccagcttgcc gtgatcgagt atgcccggca
     5641 catacttggc atagaagatg ccacaagcga agagattggc gaggggacgc atgtcatcgc
     5701 cctccttcca gagcaggagg ggatcagtga acttgggggc acgatgcgtc ttggggactg
     5761 cagcgtcacg ctcaaagaag ggacgatggt tgccggactc tacggcgaaa cttcgatcgt
     5821 ggagcggcac cgtcaccgtt acgaggtgaa ccccgcattc atcgcagata ttgaagccgc
     5881 cgggctcgtc ttctcgggtt tctgcgggca gcggatggag gtctgcgagc tcccgggtca
     5941 cccattctac ctcgcgacac agttccaccc cgagttcagg tcgagtccga cccgcccttc
     6001 cccgccctat gtagggtttg tggaagcatg caagaagaat aaatctaccc agagccaggt
     6061 ggctgagcag aatgatgaac gttgagaaat ttatcgatca ggcagtcagt gggatacgcg
     6121 atgctgccgg cggtgagaag gttgtgatgg cgctctccgg cggtgtggac tcgtcggtct
     6181 gcgcggcgct cggagcacgc gcgataggcg atcgcttgat cccgatatat gtggataccg
     6241 gactaatgcg aaagggtgag accgagcgga tacgctcgct ctttgccgat aaaaacctcc
     6301 gtgtggtgga cgccgcagac gagttcttcg aggcgctcgc gggtgtgacc gatccagagg
     6361 ggaagcgtaa ggccatcggc gagaagttta taagggtttt cgagcgcgag gcaaaacgga
     6421 cgggagcgac gatgctcctt caggggacga tctaccctga ccgtatcgag agcgaggggg
     6481 gtatcaagag ccaccacaac gtgggcggca tgcctctcaa catcgatttt aagggtgtca
     6541 tcgaacctct cgcagacctc tacaaggacg aggtgcgcga ggtcgccgcc gcgctcgggc
     6601 tcccggcgga gattcagcac cggatgccct tcccggggcc ggggcttgct gtccgggtgc
     6661 ttggcgaggt gacgagagag aagatcgaga tcgtccggga ggcaaacgcc atagtcgagg
     6721 agtgtctggt ggaggagttc ggaccctggc agtgttttgc cgctttaatc ggtcttggca
     6781 ccggtgtcaa gggggacgtc cggcttcatg gctggatagt cgctgtccgg gcggtccagt
     6841 ccagggatgg aatgaccgcc gatccactcc gtctccccta cgagaccctc tttcggataa
     6901 tggcgcggat cacagccgag atccccggtg tctcacgggt cgtctatgac gtcaccccga
     6961 aaccacccgc aacgatagag tatgagtgag gtggagagaa tggagcagga ttcacgggaa
     7021 cttgatgcac gttactacat gcccgcgttc tcccggacta tcaagatcgt ccggggcgag
     7081 gggtcacgcg tctgggatga ccgggggcgg gagtatatcg attgtgtggc tggaattgcg
     7141 gtctgcagca caggtcactg ccacccgaag gtggtccggg cgatctgtga tcaggcaagg
     7201 cagctgatcc actgttcaaa cctctactat gttccaaacc aggcggaact tgctgaacgg
     7261 cttgtagaga tcacggggct tgcaaaagcg ttcttttcaa actccggggc agaggcgaat
     7321 gatggggcga tcaagcttgc acgcatccgg accggccgga agaagtttgt tgcgttcacc
     7381 cacgggttcc atggcaggac gtgcggatcg cttgccatca ctcacaaacc cgcgatcagg
     7441 gaacctttcg aaccgctctc gcccccctgc accttcgtcg actacggtga tcttgatgcc
     7501 ttggcggaag ttgccgatgc ggataccgcc ggggtctttg tcgagccgat ccagggtgag
     7561 gcgggtgtcc tgatccctcc cgatggtttt ctccagggaa tccgggagat ctgcgatgat
     7621 tccggtgcgc tgatgatcgt cgatgaggtg cagaccggta tgggccggac agggagatgg
     7681 tttgccttcc agcacaccgg tgtcacccct gatatcgtca cgatcgcaaa agggcttgca
     7741 agcgggtttc ccatcggggc gctcgttgcg cgcgaggggc tggaattcag gaagggtgag
     7801 cacgggagca cctttgcggg cggacctgtt gcctgtgcgg cggcactcgc gactatcggc
     7861 atcataaagg gtcttctccc cgacataccc cggaaaggtg agttgttcat gaaggggctt
     7921 gccgctcaca acccgcgcgg ccgcgggctg atgatcggga tctcggtcgg tgaccgctgt
     7981 acggaggtgc agcagacctg cgctgataac ggtgttcttg tcaactgcgc cgccgatgga
     8041 aacctccgtc tgatcccgcc gcttgtgatc acggatgagg agatcgagac ggctgtcggg
     8101 gtcatcgatg cagcgcttgg ttagagcgtg tttctcaggt gcgagcggct actccttcgc
     8161 gaagaaggcc gcggacgtgg cgcgggagca cggaatcgag agggttgccc gcctggcgag
     8221 caacgagaat ccgcggcctc catctcaggc cgtgatcgag gcggggcgcg ccgcactcct
     8281 ggaggcgaac cgctacccgg atgagcgggc gtcggtcttt gttgaggctc tccgccgata
     8341 ccacggtgat taccggtttg tcaccggcgc cgggatggac ggcgtcatcg agacgctgat
     8401 ccggacggtc gtcgagcctg gtgagactgt ggtgatctcg acaccgacct tctcgttcta
     8461 cgggattgcg gccatcgcgc acggtgcccg tgtggtgaac gccccgcgga ggaaagaaga
     8521 cttctcggtc gacccggcag acttcatcag ggcgtgcggt ggcgcaaaac ttgcgttcct
     8581 ctgcaccccg aacaacccca cagggaacac cgccccgctc gaaacggttg aggagatact
     8641 ggaggggctg gaggggttgc tattcctgga caacgcctat atcgagttct cggatatgga
     8701 ttacctgccg ctcatgcacc ggcacgagaa ccttgtcatc ggccggacga tgtcaaagat
     8761 cttcgccctg gcggggctgc gggtgggcta cgcttttgtg cccggatggc ttgaaccgtt
     8821 ctacaatcgg gcggcaacac cgtttgcgct gaactcggtc tcggccgccg ctgccgctgc
     8881 cgccctcgcg gaggaagaga gggtgcgcga gacccgcgag tatgtcctgt gctggaggcg
     8941 gcggttcatc gaggagatcc cgggtgcgta tccgtcggat gcaaactttg tcatgatcga
     9001 tgttgcccca tacacaggcg acgaggtggt ggagcgtctc gcggcacggg gtgttctggt
     9061 gcggtcctgc accaccttcc ccggtctcgg cgaccactac ataagggtca gcatcggtga
     9121 cgaccgggaa aacgctctat tcatggaggc gatacgcgac ctatgatggt ggggctcacc
     9181 ggcacgccgg gaaccgggaa gagttccgtt gctgccgaac tcgaacggcg cgggcacagg
     9241 gtcgttcacc ttgttgatac ggttcaacct tatatcctgg aggaggaccg cgcccggcag
     9301 acgatggtgg ttgacaccga ccgctgggtg gcagagttcg agcccgttga cgggataatc
     9361 gaggggcatc tcgcccatct cctcccttgc gaccttgtgg tggtgctccg gtgccgccct
     9421 gacgtcttgc ggagacgcct tgaactgcgg ggttatcccc gggcaaagat cgcggagaac
     9481 gtcgaggcgg aggcgctcga tgtgatcctg atcgagacgc tcgatgaaca ccccgctgaa
     9541 cacgtcctgg aggtggatac aaccgatctc tccatcgagg agtgtgccgg gcttgttgag
     9601 cggtttatca gcggagaact cccgccatcc tttggatcga tcgactggac ggactacctg
     9661 gagaccggat tatgacgctc gaccagtaca ggccgcaggc ccagcgcatc atgcagccgg
     9721 cgataaacct ggtgcggagg gctggcataa ccccggacgc cctgacggtg ggatcgttca
     9781 tcgtatcggc gcttgccggc attgcgttct atcttggggg agtggtctct ggcaccatcc
     9841 tggtcgcgtt aaacgccgcc tttgatgcgc tcgacggagc gcttgcacgt gagatgggga
     9901 tcgccggagc ccacggggat ttcctggacc atgtcatcga cagatacgct gatatcttca
     9961 taatcaccgg gatatttgct ggcggtgctg ccccctggca gataggggta ttcgcgctga
    10021 cgggtgtcct gatgtcctcg tacctcggca cccaggcgca ggcagtgggg gtcggcaggt
    10081 tttacggcgg catacttggc cgggcggacc gcctgctgct gatcatcatt gcaggagcgc
    10141 tcaccgtcct ggttccggtt gagatctgcg gtttgaacta cctggggtgg ctcctggtca
    10201 tattcggtat tctggggcac tacaccgcca tccagcgttt cattcacgtc tggcgcgaga
    10261 ttgggaaaaa atgacctccg ccatcatggg ttgaggatgt agatgccgtg gttgaggtag
    10321 gccgcgaagg caacccagat gagatagggg acaaggagcg ctgctgcggg gatcgagacc
    10381 cggtagaagg cgactattgc cagcaggatt gcaatccaga ggagcaggat cacggtgagc
    10441 gcgaggaacg gtgcctgcag cccgaagaag gcataagacc agagaacgtt caggaataac
    10501 tggacaccaa agatccctac cgcaaccggc acaccccgct gcttccagcc cttgctccag
    10561 accaggtaga gtgcaatgcc cataaagacg tagagcaccg tccagactgg agcaaagacc
    10621 catgccggcg gggtcagctc cggttttatc agggaggcat accaggttgg aatcgccggc
    10681 atggcaaacg ccgatcctat ctttgcagct gcaaggcaga tcccgatcgc cacaatggcc
    10741 tggacggccc ggatgtatac tggtgcaggt ttcacgaggt gaaatgataa attgtcttac
    10801 ttaaccattt tggtcttcgc gcaaatgtca gccctcgagc tgacggtgtg accggagccc
    10861 cggtgcgtct ggagcagtga gcggcggcaa ccctctggaa gccgctgcca cagatggtcg
    10921 gaggaagcgg agaatatcca gaggaagagg gttactttct cttcctctcc cgcaaccgtg
    10981 cgatctcgtc gctcccgggt atcttcacca caaaagttcc gtgacagggt ttggtgtagg
    11041 ggaagacaat gttttcaccc tcgatcccga catagagcgg cggcacgccg atgatgtgga
    11101 cgtccagtat ccggtaaccc ggttcaacct cgtaatactc tttaacgttc ttccggatga
    11161 actctcgcgc ctctttaaaa gacgatccgg agagtaagac ctcgggtttc atagcttcga
    11221 gacaaccggc cattgcatca cctcgtcaag tcttcttttc agcggcattg ggttatgcac
    11281 cgccttcgcc gcgataatct cgcagggaaa ctcgatctcg ccggcgagtt cctctgcgtg
    11341 ctccccgaat ataagcgcgt agacccgcgc tttcgagata tacgccatcg ttccggcgta
    11401 tgcaacccct gaatcattgt ggatgaagac gaaacagagt ttaaaatccc gttgcttctc
    11461 tgctatctca tcgatcatat tgtcaaggtc gttcatctcg tcgatgtagc aacggccggg
    11521 atcggccacc tccatcagtt tccgtgccgc aggtgtcccg gcaatcaccg gacggcaacc
    11581 ctgtctcctc aggttgtgtg cagcgtatag cgccagactc gtctggacgg gcacctgcgg
    11641 gcacccgagc agaagcagcg cctcattctt caggttacca gtttctacca tgatctcccc
    11701 agttttttca gaacttctgg cacccgttca gggtgcgtgt agatggtgaa ccgctcgccc
    11761 cgcgagaagc ctatcagggt gatccctgca cgctctgccg tcagtatacc ccgatcggtg
    11821 gatgcagccc gggagacgac gatcgggata cctgcattgg ccgcctttgc taccattcct
    11881 gcaggctgcc ttcccgtgca accgaggacg caccccgacc tgtccagccc ccggagggtc
    11941 gcatacccca ccaccttgtc gacggtgttg tgcctgccaa cgtcgcaggc cctggtgacg
    12001 aggtcgccgt cacagaagag gacggaacag tggacgccgc cggtccgtcg ccagacatca
    12061 gactcgatcg ctgccgtgac cgcgcggaca ccgtcagcgg tgatcgtgat cgtcgaatcc
    12121 acctcctttg cctcgccaag cacccggtaa ccgccggacg actccatctc gagcaggaac
    12181 tccctcttta ccggtgcggc tatatggaca tccccgccag agacctcgac ggactcgacc
    12241 gtctccgcca gcccctcgca gacgacaaac cccgccccga gatcttcgag gcgttcaggg
    12301 cttgccacaa tggttgtaag atactctccg ttcaggaaga gccggacacg gccttcgacg
    12361 atgatactgt cctggacctc gcacacctca tccccgttca cctggatccc cgtgcggcgg
    12421 acggtctcca tcagcctgtc tcctcccgta tccagttaag gtatgggcca tacccgccga
    12481 tgatcgggat ggcgataatc tcaggcaggt cgtaggtgtg ctggccgcga atcctctcta
    12541 tcaggggatc gaggaggcgg tgctgtgtct tcatgatcag gaggcgctcg tcctctgtag
    12601 ataccgcccc ctcccaccag aaacacgacc tgacatccac aacgttcacg caggctgcaa
    12661 gacgggcatc gacgagcgct ctggcgagat tctcagcctc tccggcaggg gctgtgcaga
    12721 agaccatgat gcaatcggga ggatccatgg tttaaccttc gtatccgctt tatttaacct
    12781 tgtggggatg aagggggggc ggagcgggaa gtcccggata ttcgagccgg ggatgaagac
    12841 ggagccgcca ttcttccttg tagggcgtgt tggatgggga aaggaacttt tcccccgtct
    12901 ccgcatctct tcaggaatac ggcctttcat cgacccgccg tctcttccaa tctctcggga
    12961 attgcgtctt gcaaaaccct ggct
//
LOCUS       DAOSZX010000038        12255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4388_length_12255_cov_19.957295, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000038 DAOSZX010000000
VERSION     DAOSZX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12255
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4388_length_12255_cov_19.957295"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..349
                     /locus_tag="PK196_04625"
     CDS             71..349
                     /locus_tag="PK196_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40737.1"
                     /translation="MHALQQIRRRDVFQGAVHGFHAIDRELAVERVIRLMTAQNHLAE
                     RQGLPAARHPCLSRLPVAAPHAAHAGGDRGEECGAQRFVLQHRPASKK"
     gene            complement(338..475)
                     /locus_tag="PK196_04630"
     CDS             complement(338..475)
                     /locus_tag="PK196_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40738.1"
                     /translation="MGSNPTPRILFLAAGRHEPPLHSSVQRFQTVMGVALLIEPIDLL
                     L"
     gene            complement(451..535)
                     /locus_tag="PK196_04635"
     tRNA            complement(451..535)
                     /locus_tag="PK196_04635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Leu,seq:tag)
     gene            878..1363
                     /locus_tag="PK196_04640"
     CDS             878..1363
                     /locus_tag="PK196_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40739.1"
                     /translation="MLGELLRILAAAIITWLLFVSVDIFFRLPEKGGVSGASAVARDI
                     QAAGGDIAGGTMMGNIVSSPDASAGTLLAACGVYVAGIPGGLIAAALVFIGNRICHDP
                     GYAGTTGAVLATFVVYGFTQVGFAATDFIAGMVIAILSIQGLSHLHASRLLARLWRVR
                     Q"
     gene            1360..1599
                     /locus_tag="PK196_04645"
     CDS             1360..1599
                     /locus_tag="PK196_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 family protein"
                     /protein_id="HON40740.1"
                     /translation="MIAPYLVAVVAVFAALRAILEKNTGRKLPYANVMNFAIAASIVL
                     LLNHPLSLVAAAAYFVGSTLEANAIASTYAGGRKQ"
     gene            1596..1826
                     /locus_tag="PK196_04650"
     CDS             1596..1826
                     /locus_tag="PK196_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HON40741.1"
                     /translation="MIPALEIMFGFIAIAGAVSAALIRDSYGKLISLGILVGGIVPFI
                     VDRGYLDVAITVSLVAPIATIFVLMVVRRADA"
     gene            1826..2068
                     /locus_tag="PK196_04655"
     CDS             1826..2068
                     /locus_tag="PK196_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HON40742.1"
                     /translation="MEAIFIVGLVILVIGVLAVAFVRPKTYIARLINLEIPGWGLLLV
                     MLAYDEALALLTFVAVTAISTFVIVRLMEWRDASCR"
     gene            2059..2514
                     /locus_tag="PK196_04660"
     CDS             2059..2514
                     /locus_tag="PK196_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2106 family protein"
                     /protein_id="HON40743.1"
                     /translation="MPIISRISRVLSDFQNLVALFAGACIIVSIIGIFSLPLGFEEHQ
                     LYPKTLDPASPLYPYDRGGVPFGTTEVVAQYPENSPYVGYVTTYLTPLSQYLADHTHH
                     LGTTIVAHPGGIIDEILYNTRGLDTVVETSILFTAFAIASYLYRRREEE"
     gene            2511..3110
                     /locus_tag="PK196_04665"
     CDS             2511..3110
                     /locus_tag="PK196_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EhaG family protein"
                     /protein_id="HON40744.1"
                     /translation="MIQAYPLGLFVAFASTGIAFFALTRETDGLHRLLLTDLVEVVTL
                     AFIALVGTDLAEALILPGLVVGASELIALSEIFRIKEGYTRPPTETLQIEVMKTAPPI
                     LAALLVCYGIVLSGFSGGAVAGLGMIFYFLSRSPGERFEVIETASGYSWVLWIIAFFI
                     FMILPQYWFFAVMLAGSAILLKVTAKMSLIGTMRGGPNA"
     gene            3103..3756
                     /locus_tag="PK196_04670"
     CDS             3103..3756
                     /locus_tag="PK196_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40745.1"
                     /translation="MPDIGGADLFVLEFGDILHYFTPYTATLFIFALLFTGLAIASRP
                     ERQLDIVFGGDAYYAKQTTLPEMRFRRFMAIACGLASMGAMVSGDLFNFTLFVSLVGI
                     TNIGIVAAVKSRHVLNAAYEYGIVAMVATLPLFGGAALVLATTGTLSIPALAAGGYTV
                     PLVAKILLALGVIGEGMAPFYAAKAEMFRAPGAPYVIMCSLSSLLIFLRVVEIVITI"
     gene            3762..3965
                     /locus_tag="PK196_04675"
     CDS             3762..3965
                     /locus_tag="PK196_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40746.1"
                     /translation="MKRKTITYGLAASAIGIVVAWAALAAAIIPPFAAAMVFVIAFPL
                     LVLFLGLWWRAPGGEGDIPFIGY"
     gene            3967..4812
                     /locus_tag="PK196_04680"
     CDS             3967..4812
                     /locus_tag="PK196_04680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HON40747.1"
                     /translation="MIEYLVFAAVFGLLLLGVHRKVIARIQTRPGPPVWQEILHALKF
                     SFKSTWVPKTASGTLYVAVVLIGIGIWSAALFVVLAGESILILFAIYMLHKVVEHGYG
                     LSSGSPYGKFGGVRSVISAASEIPLFVTIAAIYLFTGSLILRDITAYQGIHGPLLLIA
                     PPVAIAMYIVVLSKMPYGPFSIVESKELVSGYKTEHFGVWRAGLAVTDGLKTFVLLYA
                     FVAIFIGAVPFWAALVIMTAVLISLSFACAITPMLSPFDSVTIQGLITGLMLVYVVCL
                     WWVMP"
     gene            4797..5051
                     /locus_tag="PK196_04685"
     CDS             4797..5051
                     /locus_tag="PK196_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40748.1"
                     /translation="MGDAMIREVVLFGGVVYIIIALVQWVQNQTVQAEAALFAGIIVV
                     LSLIHYARDEERLKKIEMAGLWLAIALFLGYALAKAGGYL"
     gene            5048..5437
                     /locus_tag="PK196_04690"
     CDS             5048..5437
                     /locus_tag="PK196_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 family protein"
                     /protein_id="HON40749.1"
                     /translation="MTGRCLYEKDLRPLKYNILTSTKHDAAVRAIARRLGVSDAKLRR
                     LLIQRFDMSLLENIETRWEMGLRYADEGDPLAKELGCELFTRYIPLLDRERMKEIYEE
                     TRALARDMPLEEAIARGRERIREAVVS"
     gene            5434..5889
                     /locus_tag="PK196_04695"
     CDS             5434..5889
                     /locus_tag="PK196_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40750.1"
                     /translation="MSILQSIKNAVRSRSIHVSYIDVGSCNGCDIEVLACLAPRYDIE
                     QYGIYVHNNPREADILLVIGSVTPQWVDKLRSIWDKIPEPKAAVAIGNCPISGCVYAR
                     KGSLTTPPVKNYIPIAAEVPGCPPRPTEILSAILSVAPLIFKDYEEKQS"
     gene            5886..6974
                     /locus_tag="PK196_04700"
     CDS             5886..6974
                     /locus_tag="PK196_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HON40751.1"
                     /translation="MKKTVDVSIPLGPMHPCWKEPVRLKCETAGERVLHTEVELGYMK
                     KGIERIMRGRPWQEVMFLAERVCGICSVVHNMVFIEAMEGISDIAVPPRAAYLRVVAN
                     ELDRIASHLIANFSYCYTIEHETLAMYLLNTRETVLDNLERLTGSRINTAYMIPGGVR
                     FDLLPEDAAVMLADLAKVEDELKRYVRIFETGPLIGLRSRGIGCMSRVDAIRAHTVGP
                     TARASGVEDCDLRLRHPTYRALDFEQVTRTEGDNFARVMVRFQEVFQSIDLIRRCVDT
                     LPAGRIRGGGLCRAGEISYSGEAPRGELTYTIKTDDFGRVVDIAIRTPSIMNIEACAH
                     YMVADAASIADVTSTFISSDPCIACNER"
     gene            6979..7590
                     /locus_tag="PK196_04705"
     CDS             6979..7590
                     /locus_tag="PK196_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40752.1"
                     /translation="MRSIIYYVREFLRPEWLKKFFFVKTAPLVTPPYFKGYPTRTEKE
                     CTGCFTCMMICPAPEAIAVLRRKERWEPEVYPGHCIRCGLCVEACPEGVLSSGRVMEV
                     AGRDATAFWHSYHLEVDESLCMRCGNCCVACPINRQIDPRLAGTGTSTSDDVIMRVKG
                     GRVQILHEEKCTGCKTCETQCPSGAMRVARMVEGVQFLDGVGS"
     gene            7587..7976
                     /locus_tag="PK196_04710"
     CDS             7587..7976
                     /locus_tag="PK196_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HON40753.1"
                     /translation="MKFLMNTGRTVNQGVTVENKGSAEYAREASTCFMHGFDMLELGV
                     DDGDPVLVRGPAGEVVMRAEASVEVEIGTVFVPYGPYANHILAADTRSTGMPDFKSHE
                     VEIEPTDEEPKSVYGLMEDLGGLAYDR"
     gene            7966..9261
                     /locus_tag="PK196_04715"
     CDS             7966..9261
                     /locus_tag="PK196_04715"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HON40754.1"
                     /translation="MIVKDAVCPFCGCLCDDIEVEVEVESGRVVGVTNACDLGATKFL
                     GGERLKTPIRREGKTWKEISYDEAIRYAAEMLLDAERPLLYGWSGTHGEAQCVGVHMA
                     ELVRGVIDNTSSVCHGPSVLAIQEVGHPGCTLGVVKNRADVVIYWGSNPIDAHPRHLS
                     RYTCYAEGFFLENSFRDRKVIVVDVRKTRTANIADEFVMVRPGGDYAVLSALRAIVRG
                     KTDTIPPTVAGVTRDQLIRVADLCLNAKFGAVYFGLGLTMIPGKNKNVRNAIELVTEL
                     NRHTKFTISAMRGHYNVYGSNEVNTWMTGYPFAIDFSRGIAFYNPGETTAVDILARKE
                     CDAALIVGSDPAAHFPRKCIEHLAEIPVVQLDPYPNATTAFCSVQIPVAVTGVDAEGT
                     AYRMDGVPIRTKKVVSSDYLSDEDVLTRMYVLMQEALKR"
     gene            9258..10958
                     /locus_tag="PK196_04720"
     CDS             9258..10958
                     /locus_tag="PK196_04720"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HON40755.1"
                     /translation="MTELLVKNAHVIDPLSGINGETMDIAIRDGKIVEEVSAAADVID
                     ARGMLTMPGGIDSHTHICGTKVNFGRYMSPEDMRAGRTPRRGPMHATSGYSVPTTYGN
                     SYRYSVMGYTTLLEGAMAPLEARHTHEEFAYTPLQDTMANTLFDGNWSIMEAIRDGDV
                     KRVAAIIAWMLTAVKGFAVKLTNPGGTEAWQWGKNVSCIRDPVPYFEVTPAEIIRSAI
                     EANELLHLPHSVHLHCNNLGTPGNYTCTLGSMGLVPDLNPKRQTLYATHVQFHAYGGS
                     GWKDFSSKSEPIANLVNMRPQIVMDMGQVMFGRTTTMTADGPMEFNLYRLHHEKWSNH
                     DVELETGSGIIPVIYRRKNLVNSIMWAIGLELALLTKSPWQCLLTTDNPNGAPFVKYP
                     EIIGLLMSRRYRDAEFAKVHPDTGSRVPLPAIDREMDWYEIAVMTRAGQARALGIQDL
                     GKGHLAPGADADVAIYPIRIDEVDPSAEFQKVIDGFSRTEYTIKRGRVVARRGECVVH
                     GSNATIWVRPKVSENYDMGKDPDFVEKFDRYYTVRMSNYPVQENYLSRSRCIETETEL
                     "
     gene            10958..11725
                     /locus_tag="PK196_04725"
     CDS             10958..11725
                     /locus_tag="PK196_04725"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HON40756.1"
                     /translation="MRVTLAMKPMGRWSIPIEAESIVPVNFLSGTDLSVWRGNRELGI
                     EEAFSVLVEGDADRAEDVEVVLSGETARLKRVGEYMGAGKITVLGDIGMHCGNFMSGG
                     MIEIQGNADGWLGREMAGGSIICRGNAADYCASGYRGGRKGMTGGSVEVFGRAGDFLA
                     ENLAGGTVIVHGDAGDMPGAEMRGGTLIIHGDCRRPAANMKNGACYVYGTARWMLPTF
                     RKVGTAQQDGRTLIQFTGDVANRGKGNLFVGNYEYLD"
     gene            11725..11874
                     /locus_tag="PK196_04730"
     CDS             11725..11874
                     /locus_tag="PK196_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PriA"
                     /protein_id="HON40757.1"
                     /translation="MVRHECGFDAPIHCKRCGRPLVNSKRTGLYCPHCGRKVSMICPG
                     CGRLW"
     gene            complement(11882..12148)
                     /locus_tag="PK196_04735"
     CDS             complement(11882..12148)
                     /locus_tag="PK196_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HON40758.1"
                     /translation="MYLVIRCPGCKTFNYVDRYQRWRLCPICGEVINVERVPIYLDAD
                     DFQDAEQVVDQLESYLHRTGKADLNEEEIRQLRAEYARWVKSRL"
BASE COUNT         2319 a         3681 c         3795 g         2460 t
ORIGIN      
        1 aaatccccat agcagcatct ataattccca cgttctttct ttatgcgctt gctcaagtcc
       61 gggatcggtt gtgcatgccc tccagcagat caggcgcaga gatgttttcc agggcgccgt
      121 ccacgggttc catgcgatcg atcgtgaact ggcggtggag cgggtcatcc gcctcatgac
      181 ggcgcagaac cacctcgccg aacggcaggg gctacccgct gcccgtcacc cctgcctctc
      241 ccgtcttcct gtcgcagcgc cgcacgccgc tcacgcagga ggcgatcgcg gagaggagtg
      301 cggtgcacag cggttcgtgc tgcagcaccg ccctgcctca aagaagtaga tcgatcggct
      361 cgatgagcag cgcgacgccc atgaccgtct ggaagcgctg caccgatgag tgcaggggtg
      421 gttcatgccg ccctgctgca agaaacagaa tgcgaggggt gggatttgaa cccacgaact
      481 ccttcgagaa cggaccctaa atccgtcgcc tttgacctgg cttggcaacc ctcgcaaagc
      541 agcgtggaat atctccggat cagggctcca ttcttgttga aaccccgaat ccagcggttt
      601 tgacccgctt cagcaaccct gccttcatat acatcagcgc cagaagcatt ataacttgtt
      661 cccgtgcgcg gtttcgcccg cttgaatctg ttttctctgg agatcctggc atacgccccg
      721 aaaaccaccc acaggattcc gtgagatggt tgcctcagga gggtggtgat gccgaaagtg
      781 cttcaggagt gtagttcggg caaatcacgc ttgaaatgtt aacaacaaaa catttattga
      841 gttggttgac tgtatttttc ctgtagttga aggaataatg ctgggcgaac tccttcgtat
      901 cctggcggcg gcgatcatca cctggcttct ctttgtgagc gtcgatatct tcttccggtt
      961 gccggaaaag gggggcgtca gtggagcgtc agctgttgcg cgagatattc aggctgccgg
     1021 cggcgacatt gccggcggca ccatgatggg aaacatcgtc agctcccccg acgcctcggc
     1081 cggaacgctc cttgctgcct gcggtgtcta cgttgccggc atccccggcg ggcttatcgc
     1141 ggccgcgctc gtcttcatcg gcaaccgcat ctgccatgac cccggctacg cagggaccac
     1201 cggtgcggtt ctcgccacgt tcgtcgtcta cgggttcact caggtcgggt ttgctgcaac
     1261 agacttcatc gccggcatgg tgatcgccat cctctccatt caggggctct cccacctcca
     1321 cgcgagcagg ctgcttgcca ggctctggag ggtcaggcag tgatcgcgcc ttacctcgtt
     1381 gccgtagtgg cggtcttcgc cgcgctccgg gccatactcg agaagaacac cggcaggaaa
     1441 ctgccctatg caaacgtcat gaacttcgcc attgctgcct cgatcgtcct gctgctaaac
     1501 catcccctct ctcttgtcgc ggccgcggcc tacttcgtcg gctcaacact tgaggctaat
     1561 gcgatcgcga gcacctatgc aggagggagg aaacaatgat cccggcgctc gagataatgt
     1621 tcgggttcat cgccatcgcc ggcgcggtct cggccgccct catcagggac tcttacggga
     1681 agttgatatc gctggggatc ctcgtcggcg gcatcgtgcc gttcatcgtg gaccgcggtt
     1741 acctggacgt cgcgatcacc gtctccctgg tcgcccctat cgccacaata tttgtcctga
     1801 tggtcgtgag gagggctgat gcgtgatgga ggctatcttc atcgtcgggc ttgtgatcct
     1861 tgtgatcggt gtccttgcgg tcgctttcgt gcggccgaag acctacatcg cgaggcttat
     1921 caacctggag attccgggct ggggcctcct gctcgtcatg ctcgcctatg acgaggccct
     1981 ggcgctcctg acgttcgtcg cggtcaccgc aatcagcaca tttgtcatcg tgcgactgat
     2041 ggagtggagg gatgcctcat gccgataatc tccaggatct cccgcgttct ctccgacttc
     2101 cagaacctcg tggctctctt tgccggtgcg tgcatcatcg tctccatcat aggcatcttc
     2161 tccctgccgc tcgggttcga ggagcaccag ctctacccaa agaccctcga ccccgcaagt
     2221 ccactctacc cctacgaccg tggaggagtc ccgttcggca caacggaggt cgtcgcgcag
     2281 tacccggaga actcacccta cgtaggatac gtcaccacct acctgacacc gctctcgcag
     2341 tacctcgcag accacacgca ccacctgggc acgacgatcg tcgcccaccc gggcgggatc
     2401 atcgatgaga tcctctacaa cacccggggg ctcgatacag tggtcgagac gagcatcctc
     2461 tttacagcgt ttgcgatcgc ctcatacctc taccggagga gagaagaaga atgatccagg
     2521 catacccgct cggtcttttc gtggcgttcg cgtcgacggg gatagccttc tttgcgctga
     2581 cgcgggagac cgacgggctc caccgcctgc tcctcaccga tcttgtcgag gtcgtcaccc
     2641 tcgcgttcat cgcactggtc ggcaccgacc ttgccgaggc gctcatcctc ccggggcttg
     2701 tggtgggtgc ctcggaactg atcgctctct ccgagatctt ccggatcaag gaaggctaca
     2761 cccggccgcc aaccgagacc ctccagatag aggtgatgaa gaccgctccc cccatactcg
     2821 ccgcgctcct ggtctgctac ggcatcgtgc tctccgggtt ctcgggcggg gcggtcgccg
     2881 ggctcggcat gatcttctac ttcctcagcc gcagcccggg cgagcggttc gaggtgatcg
     2941 agaccgcgag cggctactcc tgggtcctct ggatcatagc gttcttcatc ttcatgatcc
     3001 tcccgcagta ctggttcttc gccgtcatgc tcgcgggatc ggcaatcctt ctcaaggtca
     3061 cggccaagat gtcgctcatc ggcaccatgc ggggtggtcc gaatgcctga tatcggcggg
     3121 gccgaccttt ttgtgctgga gtttggggat atcctccact acttcacccc ctacaccgcc
     3181 acgctcttca tctttgcact gctgttcacg gggcttgcta ttgcaagcag acccgagagg
     3241 cagctggata tcgtgtttgg gggggacgct tactacgcga agcagacgac gctccccgag
     3301 atgcggttcc gccggttcat ggcgatagcc tgcggtcttg cgtccatggg ggcgatggtg
     3361 agcggtgatc tctttaactt cacgctcttt gtctcgctgg tcggcatcac caacatcggc
     3421 atcgttgcgg cggtgaagag ccgccacgtc ctcaacgctg catacgagta cgggatcgtc
     3481 gccatggtcg ccaccctgcc gctcttcggc ggcgccgcac tcgtcctcgc gaccaccggc
     3541 actctctcca tcccggccct tgcggccggc ggctataccg tcccgctggt ggcgaagata
     3601 ctcctcgccc tcggcgtcat cggggagggg atggcgccgt tctacgcggc aaaagccgag
     3661 atgttccggg cgccaggcgc tccctacgtc atcatgtgct ccctctcatc gctgttgatc
     3721 ttcctgcgcg tggtggagat tgtcataacc atctgatgat catgaaacga aagacgatta
     3781 catacggact cgcggcatcg gccatcggta tcgtcgtcgc atgggcggcg ctggcagcag
     3841 ccatcatacc gccctttgcg gcggctatgg tctttgtgat cgcgttcccc ctgctggtgc
     3901 tcttcctggg gctctggtgg cgggctccgg gcggagaagg agatataccg ttcataggat
     3961 actgacatga tcgagtacct ggtatttgca gcagtcttcg gcctcctcct cctcggggtt
     4021 caccggaaag tcatcgcacg gatacagaca agaccggggc ctcccgtctg gcaggagatc
     4081 ctccatgcgc tgaagttctc gttcaagagc acctgggtgc cgaagactgc aagcgggaca
     4141 ctctacgtcg cagtcgtgct catcggcatc ggcatatgga gcgcagcgct cttcgtcgtc
     4201 cttgccggcg agagcatcct gatcctcttt gcgatctaca tgctccacaa ggtcgtggag
     4261 cacggttacg ggctatcgtc agggtcgccc tacgggaagt tcggcggtgt gcggtcggtc
     4321 atatcagcgg catcagagat cccgctcttc gtcacgatcg ctgcgatcta cctcttcacg
     4381 ggatcactca tcctgcgcga catcaccgcc taccagggaa ttcacgggcc gcttctcctc
     4441 atcgcaccgc cggttgccat agccatgtac atcgtggtcc tctcgaagat gccctatggc
     4501 cccttctcga tcgtggagag caaagaactc gtgagcggct acaagacaga gcacttcggg
     4561 gtctggcggg caggtctggc cgtgaccgac gggctcaaga ctttcgtcct cctctatgcc
     4621 tttgtggcga tcttcattgg ggctgttccg ttctgggcgg ctctcgtcat catgaccgcg
     4681 gtcctcatat cgctctcgtt tgcctgcgcg ataacgccta tgctctcgcc gtttgacagc
     4741 gtcaccatcc aggggcttat caccgggctg atgctggtct acgttgtgtg tctctggtgg
     4801 gtgatgccat gattcgtgaa gtggtgctct ttggaggggt agtctatatc attatagcgc
     4861 tggtgcagtg ggtgcagaac cagaccgttc aggccgaggc ggcactcttt gccgggatca
     4921 tagtggttct ctctctcatc cattacgcga gggacgagga gcgcctgaag aagatcgaga
     4981 tggccgggct ctggctggcg atagccctct tcctcggcta cgcccttgca aaggcggggg
     5041 gatacctatg accgggcgct gcctctacga gaaagacctc cggcctctga agtacaacat
     5101 actgacgagc acaaagcacg atgcggccgt cagagcgatt gcgcgccggc tcggcgtgag
     5161 tgatgcaaaa ctgcgcaggc tgctgatcca gcggttcgat atgtcgctgc tggagaacat
     5221 agagacccgc tgggagatgg ggctgcgtta cgccgatgag ggcgacccgc tggcaaagga
     5281 actggggtgc gaactcttca cacggtatat accccttctg gacagggaaa ggatgaaaga
     5341 gatctacgaa gagaccagag cgctggcccg ggatatgcct cttgaggagg caatcgcgag
     5401 aggcagggag cggatcaggg aggcggtcgt atcatgagca tactccagag cataaaaaac
     5461 gccgttcggt cgagatcgat ccacgtcagt tacatcgacg tcgggtcatg caacggctgc
     5521 gatatcgagg tgctggcctg tcttgctccg cgctacgata tcgagcagta cgggatctac
     5581 gtccacaaca acccccgtga ggccgatatc ctcctggtga taggctccgt caccccgcag
     5641 tgggtggaca agctccgcag catctgggat aagatcccgg aaccgaaggc ggccgtagcc
     5701 atcgggaact gcccgatatc ggggtgtgtc tatgcccgga agggttcttt gactactcca
     5761 ccggtcaaga actatatacc catagccgca gaggttccgg gctgccctcc acgaccaacg
     5821 gagatcctct ccgccattct ctcggttgca ccgctgatat tcaaggatta cgaggaaaaa
     5881 cagtcatgaa gaagaccgtc gacgtctcca tcccgctagg gccgatgcac ccctgctgga
     5941 aggagcccgt ccgcctcaag tgcgagacgg cgggcgagcg ggtgctccat actgaggtcg
     6001 agctaggcta catgaagaag gggatcgagc ggatcatgcg cggccgcccc tggcaggagg
     6061 ttatgttcct tgctgaacgg gtctgcggga tctgctcggt cgttcacaac atggtcttca
     6121 tcgaggcgat ggaagggatc agcgatatag cggttccgcc gcgggcggcc tatctccggg
     6181 ttgtcgcgaa cgaacttgac cggatcgcca gccacctcat cgcgaacttc tcctactgct
     6241 acacgatcga gcacgagacg cttgcgatgt acctcttaaa cacccgggag acggtgctcg
     6301 acaacctgga gaggctcaca ggctcaagga tcaacaccgc ctacatgatc ccgggcggcg
     6361 tccggttcga cctcctcccg gaggacgccg cggtgatgct cgccgatctc gcaaaggtcg
     6421 aggatgagtt gaagcgctac gtccggatct ttgagaccgg gcccctgatc gggctaagga
     6481 gccgggggat cggctgcatg agccgcgtgg atgcgatccg cgcccacaca gtcggcccaa
     6541 cggcacgtgc aagcggcgtc gaggactgcg acctgcgcct tcgccacccc acataccggg
     6601 cgctggattt tgagcaggtg accagaaccg agggcgacaa cttcgcccgg gtcatggtcc
     6661 ggttccagga ggtcttccag agcatcgatc tcatccgcag gtgcgtggac accctcccgg
     6721 cgggccgtat ccggggcggc gggctctgca gggcgggtga gatctcatac agcggtgagg
     6781 cgcccagggg tgagctgacc tacaccataa agaccgacga tttcggcagg gtggtcgata
     6841 tagcgatacg tactccgtcg atcatgaaca tcgaagcctg cgcccactac atggtggcgg
     6901 acgccgcctc gatcgccgac gtgacgtcga cgttcatcag cagcgacccc tgtatcgcgt
     6961 gcaacgaaag gtgaagcgat gcgatcgatc atctactacg tccgggagtt cctcaggcca
     7021 gaatggctca agaagttctt cttcgtaaag accgcgccgc ttgtgacgcc accctacttc
     7081 aagggctacc cgacccggac ggagaaggag tgcaccgggt gtttcacctg catgatgatc
     7141 tgcccggcac ccgaggcgat cgccgtcctc cgcagaaagg agcggtggga gcccgaggtc
     7201 taccctggcc actgcatccg gtgcggtctc tgtgttgagg cgtgcccgga gggcgttctc
     7261 tcaagcggcc gggtcatgga ggtcgccggc cgggatgcaa cggcgttctg gcactcctac
     7321 cacctggagg tcgacgagag cctctgcatg cgctgcggga actgctgtgt agcatgcccg
     7381 atcaaccgcc agatcgatcc ccggcttgcg gggacgggga catcaacaag cgacgatgtg
     7441 atcatgcggg tcaagggcgg ccgtgtgcag atcctccacg aagagaaatg cacaggctgc
     7501 aagacctgcg agacccagtg cccgagcggc gcgatgcgtg tcgcccggat ggtggaaggg
     7561 gtgcagttcc tggacggggt ggggtcatga agttccttat gaacaccggg cggacggtca
     7621 accagggtgt gaccgtcgag aacaagggtt cggcagagta cgcccgggag gcctcgacct
     7681 gtttcatgca cgggtttgat atgctcgaac tcggggtgga tgacggcgat ccggtccttg
     7741 tgcgtggccc ggccggcgag gtcgtgatgc gtgcggaagc gtcggtcgag gttgagatag
     7801 ggacggtctt tgtcccctac ggcccctatg caaaccacat cctggccgcc gatacccgtt
     7861 ccaccgggat gccggacttc aagtctcacg aggttgagat cgaaccgacc gacgaagagc
     7921 cgaaatcggt ttacggactg atggaagacc ttggagggct ggcatatgat cgttaaagac
     7981 gcggtctgcc cgttctgcgg atgtctctgc gacgatattg aggtcgaggt cgaggtcgag
     8041 agcggccggg tggtcggcgt cacaaacgcc tgcgacctgg gggcgacgaa gttcctgggc
     8101 ggcgagaggc tcaagacccc gatccggcgc gaggggaaga cctggaagga gatctcctat
     8161 gatgaggcca ttcgatacgc cgccgagatg ctccttgacg ccgaacgccc gctcctctac
     8221 ggctggagcg gcacccatgg cgaggcgcag tgtgtcgggg ttcacatggc cgaactggtg
     8281 cggggggtga tcgacaacac ctcctcggtc tgccacggcc catccgtgct ggccatccag
     8341 gaggtcgggc acccgggctg cacgctcggt gtcgtgaaga accgggcgga cgttgtgatc
     8401 tactgggggt caaacccgat cgacgcccac ccgcgtcacc tctcgcgcta cacgtgttac
     8461 gcggagggtt ttttcctgga gaactcgttc cgcgaccgga aggtgatagt cgtcgatgtc
     8521 aggaagaccc ggacggcaaa cattgccgac gagttcgtca tggtcaggcc cggcggggat
     8581 tatgccgtcc tctcggcgct ccgggcgatc gtcaggggta agaccgacac catcccgccg
     8641 acggtcgcgg gcgtcacgcg ggaccagttg atccgggtgg cagacctctg cctgaacgcg
     8701 aagttcgggg cggtctactt cgggctcggc ctcaccatga tccccgggaa gaacaagaac
     8761 gttcggaatg ccatcgagct cgtcacggaa ctgaacaggc acacgaagtt taccatatcg
     8821 gctatgcgtg gccactacaa cgtctacggt tcgaacgaag tcaacacatg gatgacaggc
     8881 taccccttcg ctatcgactt ctcgcggggc atcgccttct acaaccccgg ggagacgacg
     8941 gcggtggata tcctggcccg caaagagtgc gacgccgcgc tgattgtcgg gagcgacccg
     9001 gccgcgcact tcccgcgcaa gtgcatcgaa caccttgccg agatccctgt tgtccagctc
     9061 gacccctacc ccaacgccac caccgcgttc tgcagcgtcc agatccctgt ggcggtgacc
     9121 ggggtcgatg cggaaggaac agcctaccgg atggacgggg tgccgatccg gacaaagaag
     9181 gttgtgtcga gcgattacct ctccgatgag gatgtcctca cccggatgta tgtgttgatg
     9241 caggaggctc tgaagcgatg accgagctcc tggttaagaa cgcccatgtg atcgatcctc
     9301 tcagcgggat caacggcgag acgatggata tcgccatccg cgacgggaag atcgtcgagg
     9361 aggtgagcgc tgccgcggat gtgatcgatg cccgtggcat gctcacgatg cccggcggga
     9421 tcgactcgca cacccatata tgcgggacga aggtgaactt cgggcgctac atgagcccgg
     9481 aggatatgcg ggcggggagg acgccccgcc gcgggccgat gcacgccacc tccggctaca
     9541 gcgtcccgac gacctatggc aacagttacc gctacagcgt gatgggctac accacactgc
     9601 ttgagggggc gatggcgccg cttgaggccc ggcacaccca cgaggagttc gcctacaccc
     9661 ctctccagga caccatggca aacaccctct ttgatgggaa ctggtcgatc atggaggcga
     9721 tccgcgatgg agatgtaaag agggtcgctg caatcatagc ctggatgctc acggcggtga
     9781 aggggtttgc cgtcaagctc acaaaccctg gcggtaccga ggcctggcag tggggcaaga
     9841 acgtatcatg catccgcgac cctgtgccct actttgaggt gacgccggca gagatcatcc
     9901 ggtcggcgat cgaggcaaac gaactcctcc acctgcctca ctccgttcac ctccactgca
     9961 acaaccttgg cactccgggc aactacacct gcacactggg gtcgatgggt cttgtccctg
    10021 acctgaaccc gaaacgccag acactgtatg caactcacgt ccagttccac gcctacggcg
    10081 gttcggggtg gaaggatttc tcttcaaaaa gcgagccgat cgcaaacctc gtcaacatgc
    10141 ggccgcagat agtcatggat atggggcagg tgatgtttgg ccggacaacg acgatgaccg
    10201 cggatgggcc gatggagttt aacctctacc gcctccacca cgagaagtgg agcaaccacg
    10261 atgtggagct cgagacgggt tcggggatca taccggtcat ctaccggcgc aagaacctgg
    10321 tcaactcgat catgtgggcg atcggtcttg aactcgcgct cctcacaaag agcccctggc
    10381 agtgtctgct cacgacggat aacccgaacg gtgcgccgtt tgtcaaatac cccgagatca
    10441 ttggcctcct tatgagcagg aggtaccgtg acgccgagtt tgcgaaggtc catcccgata
    10501 ccgggtcgag ggtgcctcta ccggcgatcg atcgtgagat ggactggtac gagatcgcgg
    10561 tgatgacacg ggctggccag gcgcgggcgc tcgggatcca ggatctcggg aaaggccatc
    10621 tggctccagg tgcggatgcg gatgttgcga tctaccccat ccggatcgat gaggtcgacc
    10681 cctcggccga gttccagaag gtgatcgacg ggttttcccg gactgagtac acgatcaagc
    10741 ggggcagggt cgtcgcccgc cggggcgagt gcgttgttca cggcagcaac gccaccatct
    10801 gggtcaggcc gaaggtctct gagaactatg atatggggaa ggaccctgat ttcgtcgaga
    10861 agttcgaccg ctactacacc gtccggatga gcaactaccc ggttcaggag aattacctga
    10921 gcaggagccg atgcatcgag acggagacgg aactatgatg agagtcacgc ttgcaatgaa
    10981 accgatgggg agatggtcca tcccgataga ggcggagtcg atcgtccctg tgaactttct
    11041 ctccggcacc gatctctctg tctggcgggg gaaccgggag ctcggtatcg aggaggcttt
    11101 ctcggtcctg gtggagggcg atgccgaccg ggcggaggat gttgaggtcg tcctctccgg
    11161 ggagaccgcc cggttgaaga gggtcggcga gtacatgggc gcggggaaga tcaccgtcct
    11221 cggggatata gggatgcact gcggcaactt catgagcggc gggatgatcg agatccaggg
    11281 caacgccgat ggctggctcg gccgggagat ggcgggtgga tccatcatct gccggggcaa
    11341 cgccgccgat tactgtgctt cgggttaccg cggcggccgg aagggtatga ccgggggtag
    11401 cgtggaggtc tttggccgtg ccggcgattt cctggcggag aaccttgccg gcgggacggt
    11461 tatagttcac ggcgatgcgg gggatatgcc cggcgccgag atgcgtggcg gtacgctcat
    11521 catccatggg gactgcagac ggccggcggc gaacatgaag aatggcgcct gctacgtcta
    11581 cgggacggcg cggtggatgc tccctacctt caggaaggtc ggcaccgccc agcaggatgg
    11641 gcggacgcta atccagttta ccggcgatgt ggcgaaccgg gggaaaggaa atctttttgt
    11701 cgggaactac gaatatctgg actgatggtc aggcacgagt gcgggtttga cgccccgatc
    11761 cactgcaagc gttgcgggag accgctggtc aatagcaagc gcacgggcct ttactgcccg
    11821 cactgcggca gaaaagtgag catgatctgt ccgggctgcg gccgcctctg gtgagcagcg
    11881 atcagaggcg gctcttcacc caccgggcgt actctgctct caactggcgg atctcctctt
    11941 cgttcaggtc cgcctttccc gtccggtgga ggtaagattc gagttgatcc acaacctgtt
    12001 ctgcatcctg gaagtcatcc gcatccaggt atatcggcac ccgctccacg ttgatgacct
    12061 caccgcagat ggggcagagc ctccagcgct ggtagcggtc cacgtagttg aaggtcttgc
    12121 agcccgggca gcggatgacg agatacatga tcaatgggtg tgttagggtg gctggattta
    12181 taattatcca tcctggcgat ctcccattga tgcggtgcgg tgctggaagt tgattccttt
    12241 cacccgggtt ctctg
//
LOCUS       DAOSZX010000039        11850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4590_length_11850_cov_18.797711, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000039 DAOSZX010000000
VERSION     DAOSZX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4590_length_11850_cov_18.797711"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1297
                     /locus_tag="PK196_04740"
     CDS             110..1297
                     /locus_tag="PK196_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HON40759.1"
                     /translation="MQKKQRYLGERGIIFLIALLSAFVPLSTDLYLPALPGMADLFGV
                     PASLTNLTLILFFLFFSLGLLFWGPLSDRYGRRPVLLAGLAVYIIASAGCAASWGIWP
                     LIAFRILQAAGGSAASAVSMAMVKDLYEGRRQESVLAIVQSMVVITPAVAPILGASML
                     PYTSWRGLFLALALIGAVSFIGGLLLEETASSRSTGGVREAVHRLGIVLSNPRFTYLL
                     VIFSLVSTASLAFVSASSYIYQEVFGLSEQVYSLYFALNAVALISGPFLYLWLTRHVS
                     RRKIVAAGFAVMAVSGVLVCLFGGLGPLEFALTLLPASLMGSSVRPAGAFLMLDQQRG
                     DTGSASALINCAGLVFGSAGMVLVFLAGSSLVWGLGAINLAVGAVCLAAWMMIGKRGG
                     ARE"
     gene            complement(1318..1758)
                     /locus_tag="PK196_04745"
     CDS             complement(1318..1758)
                     /locus_tag="PK196_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HON40760.1"
                     /translation="MKSIVETAGEDAQLSISVELLRAGGMDKTLLGPGEYTVLFPTND
                     AYSLFSEELLDSVRADPAKVANLVRFHVILGKLTTYELAGMEAIKTLQGEHLEISGAP
                     PGIRLNGATVIKPDIECTNGIYHIIDRVLLPRALEARLKQYGSG"
     gene            complement(1897..2457)
                     /locus_tag="PK196_04750"
     CDS             complement(1897..2457)
                     /locus_tag="PK196_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HON40761.1"
                     /translation="MGLLSEGINEVIATTCRNAAPIGIINRGGSMHMVLFRGSHTARN
                     VARDRRVVANFVFDPVIYVRTAFDDLPDDDFVTEKVGEEVFCRLRDVEAWAAFTAEVE
                     RSNTEALVVRLTPVKEEVLELRLHPVNRGFASIIEAAVHATRYVRKHDPWLLQLIEHH
                     AALARRCGGAREHEALRLLEEYIAGK"
     gene            complement(2445..3269)
                     /locus_tag="PK196_04755"
     CDS             complement(2445..3269)
                     /locus_tag="PK196_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HON40762.1"
                     /translation="MKQNLEMGRAERAQMAMMLEVCAYPKPGNVDRCHDYPDTRLEHF
                     LASAIMVRPVFDEAEKTGGRVGRLIRDAVMRTNGHSGGNTHFGAFILLVPLVLGGDIE
                     GARRVIAGTDVQDAVDFYAAFALTSVRVLKSDELDVNDPASVAAIRERGMTLADIMAH
                     SAPRDMVAREWTNGFALTRRCADLLHAHRCGRKAIVAAFMEILASEPDTFIAKKHGMA
                     AAERTMRQAAEVLAGSRDLAAFDADCVSAGINPGSIADITIAGIYLALGEGWQWDC"
     gene            complement(3238..4377)
                     /locus_tag="PK196_04760"
     CDS             complement(3238..4377)
                     /locus_tag="PK196_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HON40763.1"
                     /translation="MREAFDRFELIINDRIVRTPVAIASMAGIVDAAYVLERAEHVGA
                     AFIGGYSIDRPTMEASRQMAAAGRKEFLYDDPLEALGREIAAIQSSDVVLGINLRGSS
                     PESYAGVAEAFEDGVVYEIDAHCRQQPMLEAGCGEHLLQHPSKLFEIIRALKAEDVTV
                     SVKMRAGVAADDAALARGIWKAGADIIHVDLMDFGHSKVRQIRNASPLTLIANNSMTT
                     FDRVMDAFSHGADMISVARKSDPWTLAGLDAAITRAAEEGGWYNAPKQICRGGDIRAL
                     TFCCMPVKPCPLIPTLQKVGLSPEDYLNLKQEAVKGTPLEGGATTCFGSLAWCCKTSS
                     PCMFRNMTLESINLSPQDYMRCKRRLSETIMHRIFDEAESGDGSG"
     gene            5440..5847
                     /gene="cfbA"
                     /locus_tag="PK196_04765"
     CDS             5440..5847
                     /gene="cfbA"
                     /locus_tag="PK196_04765"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HON40764.1"
                     /translation="MAKKGMLLVGHGSKLPYNKDLVETTAKLIAEKTDEYIVRTGFMS
                     LNTPTVQEQLEAFRKEKIEMLVVVPLFLARGVHIEKDIPAILGLPEGARAGTFHMNGK
                     DVPLVYASPIGSDPLLAELMLKNAAEAIDELER"
     gene            5854..7008
                     /gene="cfbE"
                     /locus_tag="PK196_04770"
     CDS             5854..7008
                     /gene="cfbE"
                     /locus_tag="PK196_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HON40765.1"
                     /translation="MRILVLDTIHGGADLAAALRDAGHRVDEVDVYRGEVGIPVEEAL
                     VRTYDLVTAPVHLDPDHPLLQQHGPAVSHHEVVGLLIAGGAGRPFIEITGARGKTTTA
                     HAIAALMPGPGVLHTSTGTYRYPERELLWKKSITPASLIPAAREAERIGGWLVAEESL
                     GVTGAGDAAVLTSAEDYPIAAGKRRALAAKLGLLARARTVVLPQGVDLPGCIPVGSVA
                     SVEGEACRYSGYGIAGSFQNPLLSLAGYRTALSLAAATACVLGIDPSPLDGFTAIPGR
                     MAVRRDGSLLVVDNANSGTNMATTVEAARYARELAGDAPLTLVIGEEARAVCEGFPAT
                     EISRAVAAVRPDATVYVGDGEEAATLEEGFAAAREITPEGAIVLAVKTWR"
     gene            7013..8077
                     /gene="cfbD"
                     /locus_tag="PK196_04775"
     CDS             7013..8077
                     /gene="cfbD"
                     /locus_tag="PK196_04775"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HON40766.1"
                     /translation="MDYIQPRPSSIVAALYTARDLGVDVAILHGPSGCSFKHARLLEE
                     DGMHVLTTSLADNEFIFGGHDQLVRVLREAEEMFAPRRMAVVGTCVAMIIGEDLQSAI
                     LDAGIAAPAIAVDIHAGFRENIDGVVAALEPAAAAGWISAGELERQRSLLLKANEVER
                     LRGAASRAYIEPSRGDLKHIAAERLLELAGSGATGAAVMNAKKETAYIFADELIALHD
                     ACPEAAVTYIANLEDRGLPKVREDAARILAGMRGRGLEPELVGALDEYGANGQAIGER
                     IREIDPDFALLVGVPHAVPPEYTEGIEVFSITNGPRQVEPLRELGHRHVMVEIDLHPK
                     TLGVREIVESEFGAVLRSMR"
     gene            8074..9447
                     /gene="cfbB"
                     /locus_tag="PK196_04780"
     CDS             8074..9447
                     /gene="cfbB"
                     /locus_tag="PK196_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HON40767.1"
                     /translation="MKAFLVAGDRSGSGKTSITLALSALLSTGRTVQTFKVGMDYIDP
                     SYLAGVTGRPCRNLDGYVMSPAENRAVFAHGCRGAEIAVVEGVRGLFEGAEALSDLGS
                     TAAIAKLLDLPVVLVVNARSITRSAAAIVRGFQGFESGIDIRGVIANNVTGSRHREKV
                     VSAIEHYCGIPVLGAIPRSPEMELSMRHLGLVPYREGREQEEFMARIEAVKRVVAGNV
                     DLEALLSIAREIEPPAEDEAVFAVPEEPDVRIGVALDEAFNFYYADLFDVLASLGAEA
                     VPFSPIHGRLPEADGYIIGGGYPEVFGAELEANTAMREGLLEVSRNGTPIYAECGGLI
                     YLTERMVLKAGFGGREREESCDLVGVFQGETRMPARRMLGYVVGRSAAESPVGEAGFR
                     GHEFHYSSVDLAPGTRYAYRLSRGSGIRGGLDGAVRDRTIASYTHLHPVASRGMFAHF
                     VDACREG"
     gene            9503..10372
                     /gene="ilvE"
                     /locus_tag="PK196_04785"
     CDS             9503..10372
                     /gene="ilvE"
                     /locus_tag="PK196_04785"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HON40768.1"
                     /translation="MIIYLDGRFVPEEEARVSVFDHGLLYGDGVFEGIRAYNGKIFRL
                     DEHLARLYDSAKAIDLAIPLTKAEMTEVIKETLRQNSLRDAYIRPIVTRGRGDLGLDP
                     LKCAKPTVIVIAVTWGAMYGDLYEKGLRAICVSVRRTPPESMPPNVKSLNYLNNILAK
                     IEANHRGVDEAIFFDTRGHVSEGSGDNIFVVKDGVIITPPTLNNLRGITRMVVLEIAA
                     SMGITLLERDLGYFDLYTADEVFVTGTAAEVAPIREIDGRVIGNGKPGPITRQLMAAF
                     RTATQKEGTPIEG"
     gene            10460..11650
                     /locus_tag="PK196_04790"
     CDS             10460..11650
                     /locus_tag="PK196_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HON40769.1"
                     /translation="MAGSAVKIPRATCKVFGAIKALSTVKRCAVLVHGPKGCVYHINY
                     ILGMRGDRPSEVYSTCLDEHDVIFGAEEKLREAIEEIDASLHPDLIAVLSCCVSSIIG
                     EDLNAAARAASTSARVVGIEAGGFEGDFREGYSETLCRLVEEFVRGPPGEVRDRSVNL
                     VGLLRGGPDLRELLRVLGLIGVRVNATLTANAAVDDLERLGEAALNIVLCEPAGLEAA
                     KLLEQVCGTPFIVADIPVGHAATIRFLDSVADALGIGRYPLRDEGGDELCREALAGRR
                     VAIVSGPTRAIGMTRFLADLGLAPSLIVVDFDPGNLERLSGLAGPGCEILVEPEQEVI
                     RERLLAGRIDLIFGGMLERPLAASLGIEHLDMMHGSQRTLGFEGAKHIAEALTREKPG
                     HSGR"
     gene            11740..11812
                     /locus_tag="PK196_04795"
     tRNA            11740..11812
                     /locus_tag="PK196_04795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11774..11776,aa:Glu,seq:ttc)
BASE COUNT         2117 a         3656 c         3889 g         2188 t
ORIGIN      
        1 caatcatgca ggactctcac tcctgcgact ggggttcgaa tccccatagc agcatactgt
       61 tctttaccat tgcacacccc tcgccgccac ccctgatcga gacgtttcca tgcagaagaa
      121 gcagcgatat ctcggcgaac ggggcatcat attccttatc gcgctgctct cggcgttcgt
      181 tccgctctcg accgatctct accttcccgc cctgccgggg atggcagatc tcttcggcgt
      241 ccccgccagc ctgaccaacc tgaccctgat cctcttcttc ctcttcttca gcctcggtct
      301 cctcttctgg gggcctctct ccgacaggta cggccgccgt ccggtccttc tcgccgggct
      361 tgcggtttat atcatcgcaa gcgccgggtg tgccgcgtcg tggggcatat ggccgctgat
      421 cgctttccgg atcctccagg ccgcaggagg cagcgccgct tctgctgtat cgatggcgat
      481 ggtcaaggat ctatacgagg gtagaagaca ggagtccgtg ctcgcgatcg tccagtcgat
      541 ggtcgtcata accccggcgg ttgctccgat tctcggggcg tccatgctcc cctacacctc
      601 atggcgcggt ctctttctgg cgctcgccct catcggtgct gtctcgttca tcggcggcct
      661 cctgctcgag gagactgcgt cctcccgctc gaccggcggc gtgagagagg cggtacacag
      721 gctggggatc gtcctctcga acccccggtt cacgtacctt cttgttatat tctcgcttgt
      781 aagcactgca tcgcttgcct tcgtctcggc gtcgtcttac atataccagg aggtgttcgg
      841 gctctctgaa caggtataca gtctctactt tgccctaaac gccgtcgctc tgatatcggg
      901 gccgtttctc tacctctggc tcacaaggca cgtaagccgc aggaagatcg tcgctgccgg
      961 ttttgccgtc atggcggtca gcggcgtgct tgtctgcctc ttcgggggtc tggggccgct
     1021 cgaatttgcg ctcacccttc tgcccgcctc gctcatgggg agttcggtgc ggccggcggg
     1081 tgcgttcctg atgctcgatc agcagagggg ggacaccggt tctgcatccg ccctgatcaa
     1141 ctgcgccggt ctcgtctttg gcagcgccgg tatggttctg gtcttccttg ccggaagcag
     1201 cctggtgtgg ggcctggggg cgatcaacct ggcggttggg gcggtctgtc tcgcagcctg
     1261 gatgatgatt ggaaaacggg gaggggccag agagtaaaaa ggaggggggt ccagcgctca
     1321 accgctgcca tactgcttca accgtgcctc gagggcgcgc ggcaggagca cgcggtcgat
     1381 gatatggtat ataccgtttg tgcactcgat gtcgggcttt atgacggtgg ctccgttcag
     1441 ccgtatcccg ggaggcgccc cggagatctc caggtgttca ccctgcagcg tcttgatcgc
     1501 ctccatcccc gccagttcgt atgtggtgag tttgcccagg atgacgtgga acctgacaag
     1561 gtttgcaacc ttcgccgggt cggccctgac ggagtccagc aactcttccg aaaagaggga
     1621 atacgcatcg ttcgtcggga acaatacggt gtactccccc ggcccgagga gtgtcttatc
     1681 catccctcct gcccgcagca actcaacgga tatgctcaac tgcgcgtcct cccctgcggt
     1741 ctcaacgatg ctcttcatgg ataaccctga atcagcctga ggggtatata taacttaagt
     1801 aattgggctt gccggccctt cccgggaggt aataaaggag gccgtttcac ggaattcaaa
     1861 aaaagcatcc ccggggactt agatggggag aacccctcac ttccccgcga tatactcctc
     1921 aagcagcctg agcgcctcgt gctccctggc accgccgcac ctccgggcaa gggcggcgtg
     1981 gtgctcgatc agctgtaaga gccaggggtc gtgtttcctg acgtaacggg ttgcatggac
     2041 agcggcctca ataatacttg caaacccccg gttcaccgga tgcagccgga gttccagaac
     2101 ctcttccttc acgggagtga gccggacgac aagcgcctcg gtgttcgacc gctccacctc
     2161 ggcggtgaac gcagcccagg cctccacgtc acggagacgg cagaagacct cttctccgac
     2221 cttctcggtg acaaaatcat cgtccgggag gtcgtcaaac gccgtccgga cgtagatcac
     2281 cgggtcaaag acgaagtttg ccaccacccg gcggtcccgg gcgacgttcc gggctgtatg
     2341 gctcccccgg aagaggacca tgtgcatcga accgccgcgg ttgatgatcc cgatgggcgc
     2401 agcgttgcgg caggtggtcg cgatcacctc gttgatcccc tcgctcagca gtcccactgc
     2461 cacccctccc ccagggccaa gtagatcccg gctatggtga tatcggcgat agaaccgggg
     2521 ttgatcccgg ctgagacaca gtcggcgtcg aaagccgcca ggtcgcggct gcccgcaagc
     2581 acctcggccg cctgccgcat cgtcctctcc gccgcagcca tcccgtgctt cttcgctatg
     2641 aaggtgtccg gctccgaggc caggatctcc atgaacgccg caacgatcgc cttcctcccg
     2701 cagcggtggg cgtggagcag gtcggcacac cgcctcgtca gggcaaaacc gttcgtccac
     2761 tcccgtgcaa ccatatccct cggcgccgag tgggccatga tgtcggcaag ggtcataccc
     2821 ctctcccgga tggcggcaac cgaggccggg tcgttcacgt caagttcgtc gctctttagc
     2881 accctgacgc tcgtgagagc aaacgccgcg tagaaatcta cagcgtcctg gacgtccgtc
     2941 ccggcgataa cccgccgcgc cccctcgata tccccgccga gaaccagggg gacgaggagg
     3001 atgaacgcac cgaaatgggt gtttccgccg gagtggccgt tcgtccgcat gaccgcatcg
     3061 cggatcagcc tcccgacgcg gccgccggtc ttctcggcct catcaaagac cgggcggacc
     3121 atgatggccg aggccaagaa gtgttcgagc cgggtgtcgg ggtagtcatg gcagcggtcg
     3181 acgttcccgg gcttcgggta ggcgcagacc tcaagcatca tcgccatctg cgcacgttca
     3241 gcccgaccca tctccagatt ctgcttcatc gaatatcctg tgcatgatcg tctcagagag
     3301 acggcgtttg cagcgcatgt agtcctgcgg agagaggttt atactctcca gggtcatgtt
     3361 ccggaacatg cagggggaac tggtcttgca gcaccatgca agactcccga aacatgtggt
     3421 cgcgccgccc tcaagcggcg tccccttcac cgcctcctgt ttcaggttga ggtagtcctc
     3481 cggggagaga ccgaccttct ggagggtcgg aatgagcgga cagggtttca ccggcatgca
     3541 gcagaaggtc agcgcacgga tatccccgcc ccggcagatc tgtttcgggg cgttatacca
     3601 gccgccctcc tcggccgccc gggtgatcgc ggcatccagc cccgcaagtg tccagggatc
     3661 ggacttccgg gcaaccgaga tcatatccgc gccgtgggag aaggcatcca tcacgcggtc
     3721 gaaggtggtc atggagttgt ttgcgatcaa agtcaggggg gaggcgtttc tgatctggcg
     3781 gaccttcgaa tgcccaaagt ccatcagatc gacgtgtatg atatccgccc cagccttcca
     3841 tatgccccgg gcaagagcgg catcgtctgc ggcgacaccg gccctcatct tcaccgagac
     3901 ggtgacgtcc tcggccttca gggcgcggat gatctcaaag agttttgaag ggtgctggag
     3961 gagatgctcg ccgcagccgg cctcaagcat cggctgctgc cggcagtggg catcgatctc
     4021 gtagaccaca ccgtcctcaa acgcctccgc aacaccggcg tatgactcag ggctgctgcc
     4081 gcggaggttg atcccgagaa caacgtcgct gctctggatg gcggcgatct cgcgacccag
     4141 cgcttccagg gggtcatcgt atagaaactc ttttcggccg gcggctgcca tctgccggct
     4201 tgcctccatc gtggggcggt cgatcgagta accgcctatg aaagccgcac caacatgctc
     4261 cgcccgctca agaacgtagg ccgcatccac gatcccggcc atggaggcta ttgctacagg
     4321 cgtccttacg atccgatcgt ttatgatgag ttcaaaacga tcaaaagctt ctctcatacc
     4381 tctccaaccg ttggcggtga tagaggatat tgatttgcgt cgcgtcacag ggaggcgcgg
     4441 tgaaacgtct tctgcggctg accacgcggc cgcttgaggg ggcgggcagt gcgtgatgat
     4501 ttgggcggtg gaaagccgtg ccagggacgg agagaggatc tcagggagcg ttcggtcgag
     4561 gccgcgaagg gactgccaga tcccgggaca ctgtatctat gggagaatcg cccccgcggg
     4621 ggagggagat ggaacatccc cctcacctgt ctcctgcgtt tcaaccagac actcttttcc
     4681 acgtgaggtg gaaccacccc caccacccgg ccgctccgcg gcctcctccc gccccctcca
     4741 gagggggcgg gcagtctgtg gcgatagtcc ggataccgtg cacggggaga gggaaaggat
     4801 ctttcaggag tatcaggtga gaccacgacg agactgccag atcccgggac actgtatcga
     4861 tggggtatcg cccccgtggg ggtggggctg acggggaggg gggagacgga gacggctccc
     4921 ccctcacctg tctcaacgtg tcaccgggaa cggtattcta ggcgggaatc gaaccacccc
     4981 caccacccgg ccgctccgcg gcctcctccc gccccctcaa gggggcgggc agtctgtggc
     5041 gatagtccgg ataccgtgca tggggagagg gaaaggatct cggtgaggat gatcgcatcg
     5101 aaccgcggat ctgctcatcc tgcccctggc actgtatcta ttgggaatca cccccgtggg
     5161 ggtggggctc gccgggatgg ggggagacgg agacggctcc ccccgcccct gtctcctgcg
     5221 tctcaaccag acactttttt ccacgtgagg tggaaccacc cccgcctctc caatctctcc
     5281 gcagcggcgt ctcgcaaaaa ggtgcacggc ggtgccgcca cccggaatcc gtccatggag
     5341 gggcagcctc aaccccggat gcgggcggga gcctggaaaa ccgctgctcg agaaggatca
     5401 gtaattattt taattgagta tcgtagtacc ttgtaacata tggctaaaaa gggcatgcta
     5461 ctggtcggcc atgggagcaa gctcccctac aacaaggacc tcgtggagac caccgcaaaa
     5521 ctcatcgccg agaagacgga cgaatacatc gtcaggacgg gtttcatgag cctcaacacc
     5581 cccaccgtgc aggaacagct cgaggcgttc cggaaggaga agatcgagat gctggtcgtc
     5641 gtcccgctct tcctggcaag gggtgtccac atcgagaaag acatccccgc gatcctgggg
     5701 ctgcccgagg gtgcccgggc cggcacgttc cacatgaacg gaaaggacgt cccccttgtc
     5761 tacgccagcc ccatcgggag cgacccgctg ctggccgagc tgatgctgaa gaacgcggct
     5821 gaggcaatag acgaactgga acgctgaccc ggaatgcgca tactggtgct ggacaccatc
     5881 cacggcgggg cggacctcgc cgcggcgctc cgggatgccg gtcaccgggt ggacgaggtg
     5941 gacgtctacc ggggagaggt cggcatcccc gtcgaggagg cgctcgtccg cacctacgac
     6001 cttgtcaccg cgccggtcca cctcgaccct gaccacccgc tcctccagca gcacggcccc
     6061 gcggtctcgc accacgaggt tgtggggctt ctcatcgccg gcggcgcggg gcggccgttc
     6121 atcgagatca caggggcgcg ggggaagacc accaccgccc acgccatcgc cgcgctgatg
     6181 ccggggccgg gcgtcctgca cacctcgacc ggcacctacc gctaccccga acgggaactc
     6241 ctctggaaga agagcatcac cccggcctcc cttataccgg cggcgcgcga ggcggagcgg
     6301 atcggcggct ggctggttgc cgaggagtcg ctcggtgtca ccggcgccgg tgatgcggcc
     6361 gtcctgacat cggcggagga ctaccccatc gccgcgggga agagacgggc tcttgccgcg
     6421 aaactcgggt tgctcgcccg tgcacggacg gtcgtgctcc cgcagggggt tgacctcccc
     6481 ggctgcatcc cggtcgggag cgtggcatcc gtcgaggggg aggcctgccg ctactcgggc
     6541 tacgggatcg ccgggtcgtt tcaaaacccg ctcttaagcc ttgcgggcta ccgaacggcg
     6601 ctgtcgcttg cggccgcgac cgcctgcgtc ctcgggatcg atccctcccc cctggacggg
     6661 ttcacagcca tacccgggcg gatggccgtc cgccgggacg gaagcctcct ggtggtcgac
     6721 aacgcaaaca gcgggacgaa catggcgacc acggttgaag ccgcccgcta cgcaagagaa
     6781 cttgcaggcg acgcccccct gacactcgtc atcggcgaag aggcccgcgc cgtctgcgag
     6841 ggtttccccg caaccgagat cagccgggcg gttgcggccg tccggcctga tgcaaccgtc
     6901 tatgtcggcg acggagagga ggcggcgacg cttgaggagg ggtttgccgc cgcccgcgag
     6961 atcacgccgg agggcgcgat agtgctcgcg gtaaaaacct ggagatgaga tcatggacta
     7021 cattcaacca aggccgagtt ccatcgttgc ggctctttac accgcccggg acctcggcgt
     7081 cgacgtggcc atcctccacg ggccgtcggg ctgctcgttc aaacacgccc ggctccttga
     7141 ggaggacggt atgcatgtcc ttacaacatc gcttgcagac aacgagttca tcttcggcgg
     7201 ccacgaccag ctggtgcggg tgctccgtga ggcggaagag atgtttgcgc cccggcggat
     7261 ggcggttgtc gggacgtgtg tcgcgatgat catcggtgag gacctccagt cggctatcct
     7321 ggacgccggg atagccgcac cggcgatagc ggtggatatc catgccgggt tccgggagaa
     7381 catcgatggg gtggttgcgg ccctggagcc cgcggcagcg gcgggctgga tcagcgccgg
     7441 tgaactggaa cggcagcgtt cgctcctcct gaaggcaaac gaggtggagc ggctgagggg
     7501 agcggcttcc cgggcctaca tcgagccatc ccgcggagac ctcaaacaca tcgcggcgga
     7561 gcgtctcctc gaactcgcag ggagcggcgc cacgggtgct gcggtgatga acgccaagaa
     7621 agagaccgcc tacatcttcg ccgacgagct gatagcgctc catgatgcct gccccgaggc
     7681 cgcggtcacc tacatcgcga acctggaaga ccgcgggctc ccgaaggtca gggaggacgc
     7741 cgcccgcata ctcgccggga tgagaggccg cggtctcgag cccgaactcg tcggggcgct
     7801 ggacgagtac ggggcaaacg gccaggcgat cggggagcgg atccgtgaga tcgacccgga
     7861 tttcgccctc ctggtcgggg ttccccacgc cgtccccccg gaatacacag aggggataga
     7921 ggtcttctcg atcacgaacg gcccccggca ggtcgaaccg ctccgggaat tggggcaccg
     7981 ccacgttatg gtcgagatcg acctccaccc aaagacgctg ggcgtccgtg agatcgtcga
     8041 gagcgagttc ggggcggttc tgcggagcat gcgatgaagg cattccttgt tgccggcgac
     8101 cgctcgggga gcgggaagac gagcatcacc ctggcgctct cggcgctgct ctcgaccggg
     8161 aggactgtcc agacgttcaa ggtggggatg gactacatcg acccctcata ccttgctggg
     8221 gtgaccgggc ggccctgccg gaacctggac ggctacgtga tgagcccggc ggagaaccgg
     8281 gcggtctttg cccacggctg ccggggagcg gagatcgccg tcgtcgaggg cgtccggggg
     8341 ctgtttgagg gggcagaggc tctctcagac ctggggagca cggcggcgat agcgaaactg
     8401 ctcgaccttc ccgtggtgct ggtggtgaac gcccggagca tcacccggag cgcggcagcg
     8461 atagtgaggg ggttccaggg gtttgagagc ggcatagata tccggggggt catcgcgaac
     8521 aacgtcactg gaagcaggca ccgggagaag gtcgtatccg cgatcgagca ctactgcggc
     8581 atcccggttc tcggcgccat cccccgttcg ccggagatgg aactctctat gcggcacctg
     8641 ggtcttgtcc cgtaccgcga gggccgggag caggaagagt tcatggcgcg gatcgaggcg
     8701 gtgaaacggg tggttgccgg gaacgtcgat ctcgaggcgc tgctctcgat tgcccgtgag
     8761 attgagcccc cggcggagga tgaagcggtg tttgcggttc cagaggagcc ggatgtgagg
     8821 atcggggtcg cgctggatga ggcgttcaac ttctactacg ccgacctctt cgacgtcctg
     8881 gcatcgctcg gggcggaggc tgtcccgttc tccccgatcc acggccgcct gcctgaggcg
     8941 gacggctaca tcatcggcgg cggttacccg gaggtctttg gagcggaact cgaggcgaac
     9001 accgcgatga gggaggggct cctcgaggtc tccaggaacg ggacgccgat ctacgcggag
     9061 tgcggcggtc tcatctacct gacggagcgg atggtgctca aagccgggtt tgggggccgg
     9121 gagagggagg agtcctgcga ccttgtcggg gtgttccagg gcgagacccg gatgcccgcg
     9181 aggcggatgc tcggctacgt ggtcgggagg agcgctgcag agagccctgt gggcgaggcg
     9241 gggttccggg gccacgagtt ccactacagc agtgttgacc tggcgccggg gacgcgctac
     9301 gcctacaggc tgagcagggg gagcgggatc cgcggcgggc tcgacggcgc cgtccgcgac
     9361 cggacgatcg cgagttacac gcatctccac ccggttgcaa gccgggggat gtttgcgcac
     9421 ttcgttgacg cctgccggga gggctgagaa cggggtgcac gaaacgcaag acatacaaag
     9481 aaatcgatac cacactactc ccatgataat ttaccttgac ggcagattcg tcccggagga
     9541 ggaggccagg gtctcggtct ttgaccacgg gctcctctat ggtgacggtg tttttgaggg
     9601 gatacgcgcc tacaacggta aaattttccg tcttgacgag catctcgcac ggctctacga
     9661 ctcggcaaag gcaatcgatc tcgccatccc gctcacgaag gcggagatga cggaggtcat
     9721 caaggagacg ctgcggcaaa acagtctgcg ggacgcctac atccgcccga tcgtgacgcg
     9781 gggcaggggc gatctggggc ttgacccgct gaaatgcgca aaaccgaccg tcatagtcat
     9841 cgccgtcacc tggggggcga tgtacggcga cctctacgag aaggggctcc gggcgatctg
     9901 cgtctcggtc cggcgcacgc cgccggagtc gatgccgccg aacgtgaaga gcctcaacta
     9961 cttaaacaac atcctggcaa agatcgaggc gaaccaccgg ggggtcgatg aggcgatctt
    10021 cttcgatacg aggggtcacg tctctgaggg gtccggggac aacatatttg tcgtcaagga
    10081 cggtgtgatc atcactccgc cgacactgaa caacctgcgc gggatcaccc ggatggttgt
    10141 gctggagatc gcggcatcga tggggatcac cctccttgag cgcgatctcg ggtatttcga
    10201 cctttatacg gctgacgagg tctttgtcac ggggacggcg gcggaggttg cgccgatccg
    10261 cgagatcgac gggcgggtga tcgggaacgg aaagcccgga cccatcacca ggcagctgat
    10321 ggccgccttc aggacggcca cccagaagga aggcaccccg atcgaggggt aggcttccac
    10381 tttttttcct cccggcgagg ctgcaggtat aactacctga aggctgaaga gttgatcatc
    10441 aggaggtgtg ccgcttttta tggcgggcag cgcggttaag atcccgaggg cgacgtgcaa
    10501 ggtctttggc gcgataaaag cgctttctac ggtgaagcgg tgcgctgtgc tcgttcacgg
    10561 cccgaagggc tgtgtatatc atatcaacta catcctgggg atgcggggcg accggccatc
    10621 ggaggtctac tcgacctgtc ttgatgagca tgatgtcatc ttcggcgccg aggagaaact
    10681 ccgggaggct attgaggaga ttgatgcgag ccttcacccc gacctgatcg cggtgctctc
    10741 atgctgcgtc tcaagcatca tcggcgagga tctaaacgcc gcggcgaggg ccgcctcaac
    10801 gtctgcacgg gttgtcggca ttgaggccgg cgggtttgag ggggatttca gggagggtta
    10861 cagcgagacc ctctgcaggc tggtcgagga gtttgtccgg gggccgccgg gcgaggtcag
    10921 ggatcggtcg gtcaacctgg tcggtcttct ccggggcggg ccggacctga gggaacttct
    10981 gcgggtcctg ggtctcatcg gcgtccgggt caacgcgacg ctgaccgcga acgcagcggt
    11041 cgatgacctt gagcgcctgg gggaggcggc gttaaacatc gttctctgcg agccggccgg
    11101 gcttgaggcg gcgaagttgc ttgaacaggt ctgcggcacg ccgttcatcg tcgccgatat
    11161 ccctgtgggt catgccgcca ccatccggtt cctggacagc gttgccgacg ccctggggat
    11221 tgggcgttac cctctccggg atgagggggg cgacgaactc tgccgggagg ccctggccgg
    11281 gaggcgggtg gcgatcgtaa gcgggccgac gagggcgatc gggatgaccc ggttcctggc
    11341 ggacctgggg cttgcgccgt cgctcatcgt tgtggacttc gacccgggca acctggagcg
    11401 gttgagcggc ctggccgggc cggggtgcga gatcctggtt gaaccggagc aggaggtgat
    11461 ccgggagagg cttttagccg ggaggatcga cctgatcttt ggcggcatgc tcgagcggcc
    11521 gctggccgcc tcgctcggga tcgagcacct ggatatgatg cacggcagcc agaggacgct
    11581 cgggtttgaa ggcgcaaagc atattgccga ggcgctgacg agagagaagc cgggtcacag
    11641 cggcaggtag aggcggagcg gttttgagat ccggcccggg atcgctgcgg cacaactttt
    11701 ttatttatcg gtgcccaagt tgtatggcac attgcacggg ctgctatagt gtaggggcca
    11761 atcatgtcgg cctttcacgc cgacgactgg ggttcaaatc cccatagcag catctataat
    11821 tcccacgttc tttctttatg cgcttgctca
//
LOCUS       DAOSZX010000040        11809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4603_length_11809_cov_21.316488, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000040 DAOSZX010000000
VERSION     DAOSZX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_11809_cov_21.316488"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1568
                     /locus_tag="PK196_04800"
     CDS             234..1568
                     /locus_tag="PK196_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HON40770.1"
                     /translation="MPDAIPQTMDRNAIGLLLLAGIIALGCIAAGCTGTEKAAPADST
                     PPAGEGTASPDDVAESISNVTDGNNRFALDLYRQISSDPAYAGRNIFFSPYSISSALA
                     ITYEGARGTTADEIRSVLSLPADDAVRRQGFAALNAALNRGSDNYTLHIANALWAEET
                     YRFLPDYIEAAERWYGANVTNLDFVSDAEDSRETINRWVEEKTEDRIRDLLPPGSIDS
                     ITRLVITNAIYFKGDWVREFNPDETTDEEFRVSPEKTVTVPMMHRTDEEAIYPYTETD
                     TLQVLEMPYAHGNETGISMIVLLPKDGNLTALEETLDADTIAELRNSLVEQRVRVVFP
                     KFRLETGYTLSQILADMGMPAVFSADADLSGMDGTDMLFVNEVYHKAFVDVNEEGTEA
                     AAATGVVVSLKGAPGGDTTPVFRADHPFVFLIVDKDNGTILFAGRVVNPEDQ"
     gene            complement(1707..2462)
                     /locus_tag="PK196_04805"
     CDS             complement(1707..2462)
                     /locus_tag="PK196_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40771.1"
                     /translation="MTAEVVAALRALGMNEYEARVYSTLVGLQKATARDLHEISGVPR
                     GRIYEILNDLARRGFIGVEEGSPTSYYVLDIDLVFDRLKDDQIRSLDLTREALKRLSV
                     KPRLPPDSFFILRSDWAIENHISALFRRVKKSMVILCYNPEFLRRHYAAIKPLEKKID
                     LYVVVRGKEDYSEIKLPIYEARGAVIDLLDTPPVNDPLIDLRQDECTILVDGRDFLII
                     ATAGSGRHAVIGSDMPIIGYLQKTVVERLEEAA"
     gene            2958..3377
                     /locus_tag="PK196_04810"
     CDS             2958..3377
                     /locus_tag="PK196_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40772.1"
                     /translation="MNCATTCNTGLNRGSREPPCRDPVHREVSCSEPHIEETDQVWFE
                     AGFLPPEEHRLAVALLHHLAMDEDPATPLLSSILSGMAGAPEFDRGYRIFDRSKLHRI
                     ASEYGIKQDGMSETAVAHAVTLAIINEYREEMEGDRR"
     gene            3374..5638
                     /locus_tag="PK196_04815"
     CDS             3374..5638
                     /locus_tag="PK196_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HON40773.1"
                     /translation="MKNPYEWLASAINNRTWTVAGVAAAVFILALLGLSMVSMETGED
                     TYLDKTTPRGALLDHYMETYGSDAIMLLYEADNVRDPEILRYIDNLQEDLRGERYVES
                     VSGVVDALKQANGGILPSSKAEIDAIIAQVPAETVEQMMPSGLMTIGVINLEPGVGLD
                     TQKQVLGNVRSTIAISNPPPGLTVTVTGNPAFAQDMMEDMGTETGTLILVAMVLMVVA
                     VGLLFSHVRYSLLPVAIVAIGLISTFGLMGLFGIPISMVVVGSFPVLIGIGIDYGVQF
                     HARFDDEVRQKTIPEAVWATVTQMGPSVLIAMSATALGFIAMFFAPVPMVADFGIVCT
                     IGIASCYIAALVIVPIFAIVTRYQPKNGKPTTGDATSQPSKGALMERYDRFLGRLAYT
                     VAKHPLPVIMLFAVVAAGGFYLDQSVPVSADEKTFVPDDMPALLSLEKLIRVMGSTTT
                     IPVVVSADDVLSPDTLAWIDRFGIYEQEHNDKITGVTSVATLVRDYNGGVLPSTEREV
                     DTVVARIPESTLSRYLNGNMEAVLEFSTVEMEMSVARDLVKRMQDDVAWSNPPAGVTV
                     KITGSLEMFAAVMDDISESRVYMTVIGFAFILIFLILVYRKFNAISPIIPIVFIVGWN
                     GGIMYMLGLDYTPLTAVLGSMTIGVASEYTVLIMERCEEELARGTPFLEAIQTAVQKI
                     GTAITVSGLTTVFGFAALIVSNFNIISNFGIVTVLSVAFSLLGAIVVMPAILALMYRF
                     TGRSRTPSRIAAPE"
     gene            complement(5753..6541)
                     /locus_tag="PK196_04820"
     CDS             complement(5753..6541)
                     /locus_tag="PK196_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON40774.1"
                     /translation="MNDDLKAAVIEQCRKMEIPLVGVANTERWENPPFLPWMPEDFYP
                     QSIFPGARSVIVIGLPIHLPVIETAPSIYYHELYRTVNTLLDQYTYRLASFLNDRGHP
                     SVFVPRDGYGSIEVLLKNPVAFFSHRHAAFLAGLGTFGVNNTILTPEYGPRVRFASVI
                     TTAEIPADPLFPENLCTHCMLCVHSCPVGALDELDYPDGLTDSAACAANSARLSRRHI
                     SPCGICIRVCPVGEDRRHYGRSAAAGEDPRHIRAREHVQKYGGL"
     gene            complement(6582..6860)
                     /locus_tag="PK196_04825"
     CDS             complement(6582..6860)
                     /locus_tag="PK196_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HON40775.1"
                     /translation="MRVRVKAFARFREILGGEFSIDLPDGAQMEAVLAVLRERAADEA
                     AAVFDETGILRPHIILMLNGKRANRNDIGSLTLADGDEVALFPPVAGG"
     gene            7028..7351
                     /locus_tag="PK196_04830"
     CDS             7028..7351
                     /locus_tag="PK196_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HON40776.1"
                     /translation="MKLPCQSIVWNVLPAIRAAIAEELIALGVSQLEAARLLEMTPPA
                     ISQYRTGKRGYRIVFEGDARGPVSQLARDLKDGRVEDLASRICEICIQIRGENLLEGS
                     CGTDA"
     gene            7366..8184
                     /gene="larE"
                     /locus_tag="PK196_04835"
     CDS             7366..8184
                     /gene="larE"
                     /locus_tag="PK196_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HON40777.1"
                     /translation="MEAECSREMIIETLKKHERLLIAFSGGVDSSLLAALAVQALGRE
                     RVRCALLDSPLIPRRDVEEAKETAAELGLPLEVVAFPILADPAFRENRADRCYTCKKA
                     AFRLLKEVARREGIETIADGTNLSDLGEYRPGLAASDEEGVCHPLAEAGATKDDIRRI
                     ARECGLPCWNRPAAACLATRIPYGSEITEEVLSMVEAAEDALCDRGFSGVRVRVHGSL
                     ARIEVPVDEMARLFEMRRDIAAEFHRMGFVYVTLDLAGYRSGSMDEANEHLHQP"
     gene            8270..>9228
                     /locus_tag="PK196_04840"
     CDS             8270..>9228
                     /locus_tag="PK196_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HON40778.1"
                     /translation="MPCAIVTGDVFSGHGDPGHPESQARLDAALTGVPAGTDRITPVR
                     ATPGDLALVHTERHIETIRHLCRDLPPGRALYLDPDTYITAGSFDAALYAAGGAWQAV
                     ERALEGEHCFALVRPPGHHATPDRAMGFCIFNNVAIAVEKALREVERVAIVDWDVHHG
                     NGTQAAFYTTDRVLYCSVHQAGIFPGTGWPEERGAGRGAGYNINLPVVAGSTGADYAL
                     VFRRVFAPALARFEPDALVVSAGQDALFDDPLASLLLRPEDFGALTAMLLEVYDGALA
                     LVLEGGYSVSHPAAIEAIIAALDGARFNPGPGEPKETTRQLVEA"
     assembly_gap    9229..9328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9329..9706
                     /locus_tag="PK196_04845"
     CDS             <9329..9706
                     /locus_tag="PK196_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40779.1"
                     /translation="GAFEFYLGGSAGPSLYVRLVGGRLLYEWAANGSYSGAVMEAAPT
                     PEEWARFRETADRLGVREWEPQYLSAHSCCDVTYWYLKMELDGQKIVVQGSDAYPAST
                     GPNVSKEFREFFRAVKNLVGLEP"
     gene            complement(9712..10413)
                     /locus_tag="PK196_04850"
     CDS             complement(9712..10413)
                     /locus_tag="PK196_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON40780.1"
                     /translation="MDRSLEEALDVISSLTDRAEILSDSLHSLFISEEACHSFDLQYT
                     VHAPTADINIASENERLRKAAIQVIGDLAEVCDRIGAVRLVIHPGHVWEEERRGAAQQ
                     ALDRSLDDLAAMQQEVNVRFAVENMGTWDVCFFREPDFLGRLADTGLGFALDVGHAHA
                     NGNLETFLELGGAIHVHLHDNCGNRDDHLACGEGNINFRRVLAAIPAGATKVVEPVSF
                     QKFEQSLDYLGRLTL"
     gene            complement(10922..11563)
                     /locus_tag="PK196_04855"
     CDS             complement(10922..11563)
                     /locus_tag="PK196_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HON40781.1"
                     /translation="MLFAVLALGSLICPVTGQEISGSEVWRSGVGDPVMAVALSEDGL
                     YSPVITERNIYVYDQNATVLWRYPVSHGKGVAVSSDSERIAAAGDHLLLFDREGNIIW
                     RYKPDSRIRDVALTADGRTICTATDAGLRVFSLNKERTVANVSWTFDAKDRIKSVSVD
                     GGVRVSLPAGRRVMYTSSAGMGASSGLTERVIAESRRSSPTTDRRLPQHPASR"
     gene            complement(11594..>11809)
                     /locus_tag="PK196_04860"
     CDS             complement(11594..>11809)
                     /locus_tag="PK196_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263046605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON40782.1"
                     /translation="GRVYVIDADAASRGGPRIVDALEEAAAIFHPDLFEAGSPKETTA
                     ARSPGFNVITLACALLVLLLIHERGRK"
BASE COUNT         2402 a         3545 c         3510 g         2252 t
ORIGIN      
        1 ccccacctcc cctctctcct caatgccgtc tcgcaaaaaa ccgcatgatg ctgctgtgac
       61 cagaggcata ccgcatgaag atgggggcag catcttcata cagatcccgg ccaccccccg
      121 gaatctgtcc atggatcgtc cgtggactgt ccacggagag gtttatcaaa ccagtgtggc
      181 tgttggtttg atggcctctt agaattcctt cgtcgttaac gccaggtata actatgcccg
      241 atgccatacc gcagaccatg gacagaaacg ccatcggcct gctgcttctc gcaggcatta
      301 tagcgctcgg atgcatcgcc gccggatgca ccgggacaga aaaggccgcg cctgcggatt
      361 ccacccctcc tgccggagag gggaccgcga gtccggatga tgttgcagag agcatcagca
      421 acgtcacgga tgggaacaac aggttcgcgc tcgatctcta caggcagatc tcaagcgacc
      481 cggcatacgc cggcaggaac atcttcttct ccccctacag catctcttcg gcgctcgcga
      541 tcacctacga gggtgcccgc ggcacaaccg cagacgagat caggtcggtg ctcagcctcc
      601 ccgccgacga tgccgtccgg cggcaggggt ttgccgcgct caacgccgcc ctgaaccggg
      661 gcagcgataa ctacaccctc cacattgcaa acgccctctg ggcagaggag acctatcggt
      721 tcctcccgga ctacatcgag gcggcggaac gctggtatgg ggcaaacgtc acgaacctgg
      781 acttcgtgag cgacgccgaa gattcgcggg agacgatcaa ccggtgggtg gaggagaaga
      841 ccgaagatag gatacgcgac ctgctcccgc cgggttcgat cgactcgatt acccgtctag
      901 tgatcacaaa cgcgatctac ttcaagggag actgggtcag agagttcaac ccggatgaga
      961 caactgatga agagttccgg gtttcgcctg agaagactgt gaccgtcccg atgatgcaca
     1021 ggactgatga ggaggccatc tatccctaca ccgagaccga taccctccag gtgctcgaga
     1081 tgccctacgc gcacgggaac gaaaccggga tatccatgat cgtcctcctc ccgaaggacg
     1141 gcaacctgac ggcattagag gaaactcttg acgctgatac gattgcagaa ctccggaatt
     1201 cgctggtcga gcagcgggtg agggttgtct tcccaaagtt caggcttgag accggctaca
     1261 ccctgagcca gattctcgcg gatatgggta tgccagcagt cttttccgct gatgccgacc
     1321 tctcggggat ggacggcaca gatatgctct tcgtcaatga ggtctatcac aaagccttcg
     1381 tcgatgtgaa cgaggagggc accgaggccg cggccgcgac cggtgtggtc gtgagcctca
     1441 agggtgcacc cggcggcgac accacacccg tattccgggc ggatcacccg ttcgtcttcc
     1501 tgatcgtcga taaggataac ggcacgatcc tctttgcggg ccgggtcgtg aacccggagg
     1561 atcagtagta agggcgggaa agtatggcca acctggctat aacgtccact ttttccatga
     1621 agagaaggca gcgccagggt tttgggaagg gaaatgacgt gtgaaagcat atccgggaga
     1681 ggcggcggtt tccggacgcc tctggctcac gccgcctctt ccagccgctc gacgaccgtc
     1741 ttctggaggt agccgatgat cggcatatcc gagcctatca ccgcgtgccg gccggaaccg
     1801 gcggtggcaa tgatcaggaa gtcccggccg tcgaccagga tcgtgcactc gtcctgcctt
     1861 aagtcaatca gggggtcgtt caccggcggc gtatccagaa gatcgatcac agcccccctg
     1921 gcctcgtaga tcgggagttt gatctctgag tagtcctcct ttccgcgtac aacgacatac
     1981 agatcgatct tcttctcaag cggtttgatg gccgcatagt gtctgcggag gaattccgga
     2041 ttgtaacaga ggatcaccat gctcttcttc acccggcgaa atagcgctga tatatggttc
     2101 tcgatcgccc agtcgctccg gaggatgaag aacgagtccg gagggagacg cggtttgacc
     2161 gagagtctct tcagcgcctc gcgggtcaga tcgaggctgc ggatctgatc atccttgagc
     2221 cggtcaaaga ccagatcgat atcgagaacg tagtaggatg tgggagaccc ctcttcaacc
     2281 ccgataaacc cccggcgcgc aaggtcgttt aagatctcgt agatccgccc ccgtgggacg
     2341 ccgctgatct cgtggaggtc ccgtgcggtc gctttctgaa gtccgacaag cgtggaatag
     2401 acgcgtgcct cgtattcgtt catcccgagc gcacgcagcg ccgcgacaac ctcagcggtc
     2461 attgcacctc tgttgggttg caacacactt atgagttgcg aagagagacc acgtacaccg
     2521 ccaggagggt cgatctctcc ccgccagagc atctttgatc gggcagccgt cagcacgccc
     2581 gggataaacc ctcctttcgc gaggctggct gcccggcagg cgatggatga catataacag
     2641 agcgcaggaa cagacagtat atcagatcta actccccggc cgtaccacgc ttcgagaaac
     2701 caggtgatcc acccatcgac aagatcccgt gtcacaaccc gcggcagggg cggcggcaat
     2761 gcgcgcgtct gaaaccgcag cccgaaccga tgctcattcc ggatttctgt tccccatatg
     2821 gcctaccagt aaacccctca actcccacag cagtcccttt gcacttgatc ggttcctatt
     2881 cggggttgca gcaggttcat atcccgtccc ccgaaaacat tacctccagc tgatgaacca
     2941 tttatgagag gagagacgtg aactgcgcaa ccacctgcaa cacaggactc aaccgtggtt
     3001 ccagggaacc accctgcaga gacccggtcc accgtgaggt gtcctgcagc gaaccgcata
     3061 ttgaggagac tgaccaggtc tggtttgaag ccggatttct cccgccagag gagcatcggt
     3121 tagccgtggc cctgctccac caccttgcca tggacgaaga ccccgccacc cccctgctct
     3181 catccatact gagcggaatg gcaggggccc cggaatttga tcggggttac cggatctttg
     3241 atcggtcgaa actccaccgc atcgcatcag aatacgggat caaacaagac gggatgagcg
     3301 agaccgcggt cgcccatgcg gtgacccttg cgatcataaa tgaataccgc gaggagatgg
     3361 aaggggaccg cagatgaaga acccttacga atggcttgcc agtgccatca acaaccgtac
     3421 ctggacggtt gcaggggtcg ctgcagccgt ctttatcctg gcgctcctgg gcctctcgat
     3481 ggtctcgatg gagacaggtg aggataccta tcttgacaag accacgccac gcggagcgct
     3541 cctggatcac tacatggaga cctacgggtc tgacgcgatc atgctcctct acgaggcaga
     3601 caacgtcaga gaccccgaga tcctcaggta tatagacaac ctccaggaag acctcagggg
     3661 cgagcggtat gtcgagagcg tctccggcgt tgtcgacgcc ctgaaacagg caaacggtgg
     3721 catcctgcca tcttcaaaag cagagatcga cgcgatcatt gcacaggttc ccgctgaaac
     3781 ggtcgagcag atgatgccct cagggctcat gacgatcggg gtcatcaacc ttgaacccgg
     3841 tgtcggcctg gatacacaga aacaggtcct cggaaacgtc aggtcgacca tagccatctc
     3901 aaaccccccg ccaggcctta ccgtcacggt caccggcaac ccggcattcg cccaggatat
     3961 gatggaagat atggggaccg agacgggcac gctgatcctt gtggccatgg ttctgatggt
     4021 cgttgctgtg gggctcctct tctcgcacgt ccgatacagt ctcctccccg tcgcgatcgt
     4081 ggccatcggc ctgatctcaa ccttcgggtt gatggggcta ttcggcatcc cgatcagcat
     4141 ggtggttgtc ggatcgttcc cggtgctcat agggatcggg atagactatg gcgtccagtt
     4201 ccacgcacgg ttcgatgacg aggtccgaca gaagacgatc ccggaggccg tatgggccac
     4261 cgtcacccag atgggaccct ctgtcctgat cgcaatgtcg gcaacggcgc tagggttcat
     4321 cgcgatgttc tttgcccctg tcccgatggt ggccgatttc gggattgtct gcaccatcgg
     4381 gatcgcctcc tgctacattg cggcgctcgt catcgtccct atctttgcga tcgtcacccg
     4441 ctaccagcca aagaatggga agccaaccac gggagacgcc acctcacagc catcgaaagg
     4501 ggccctcatg gagcggtatg accgcttcct cggccgtctg gcctacaccg tcgcaaaaca
     4561 tccacttcca gtaatcatgc tgtttgcggt ggttgcagcg ggagggttct accttgacca
     4621 gagcgtccct gtcagtgcgg atgagaagac cttcgtcccg gacgacatgc ccgctctcct
     4681 ctccctcgag aagctcatca gggtgatggg atcgacgacc accatccctg tcgttgtgag
     4741 cgcagatgac gtcctctcgc ctgacactct cgcctggatc gatcggttcg ggatatacga
     4801 gcaggagcac aacgacaaga tcacaggggt gaccagcgtg gccaccctgg tcagggacta
     4861 caacggtggt gtgctcccct cgacagagag ggaagtcgac acggtcgttg cccggatccc
     4921 ggagtcgacc ctgagccgct acctcaatgg gaacatggag gcggtgctcg agttctcgac
     4981 ggtcgagatg gagatgagcg tcgcccgtga ccttgtcaag aggatgcagg atgatgttgc
     5041 gtggagcaac ccgccggccg gcgtgacggt gaagatcacc ggcagccttg agatgtttgc
     5101 ggccgtaatg gacgatatca gcgagtcacg cgtgtatatg acagtgatcg ggtttgcctt
     5161 catccttatc ttcctgatcc tggtctaccg gaaattcaat gccatctcac cgatcatccc
     5221 gatcgtcttc attgtgggct ggaacggcgg gatcatgtac atgctcggcc tggattacac
     5281 cccgctgacc gccgtccttg ggtcgatgac gataggcgtt gcatcagagt acactgtcct
     5341 gataatggag cgttgcgagg aggaacttgc ccgtggcacg ccattcctcg aggctatcca
     5401 gacagccgtc cagaagatag ggacggcaat cactgtctcg gggctgacaa cggtcttcgg
     5461 gttcgccgca ctcatcgtct caaacttcaa catcatcagc aacttcggga tcgtgaccgt
     5521 cctctcggtg gcattctcgc tcctgggcgc gatcgtggtg atgccggcta tcctggcatt
     5581 gatgtaccgc ttcaccgggc gttcccgcac cccgagcagg atcgccgccc ccgagtgagt
     5641 cggaaaaaat cacctcactt tcttttatca gcgcctctga acccaggcag gtcggagacg
     5701 gcaacgagtc tccctgcgat gaagcccggg agaccgtctc gccgggaggc tgctaaagcc
     5761 caccatactt ctgcacgtgc tccctggccc ggatgtgacg cgggtcttca ccggcagcag
     5821 cgctgcgacc gtagtgccgg cggtcctctc ccaccgggca gaccctgatg cagatcccgc
     5881 agggggagat gtggcggcgg ctgagtcggg cgctgtttgc agcacaggcc gcgctgtcgg
     5941 tcagaccgtc ggggtagtcc agttcatcga gcgcacccac gggacaggag tggacgcaga
     6001 gcatgcaatg ggtgcagagg ttttctggaa acagcggatc ggccgggatc tcagctgtgg
     6061 tgatgacgga ggcaaaccgg acacgcgggc cgtattccgg cgtgaggatg gtgttgttca
     6121 cgccgaaagt tccaagcccg gcgagaaagg cggcgtggcg gtgtgagaag aatgctacgg
     6181 ggttcttcag gagcacctcg atgcttccgt aaccgtcccg cgggacgaag accgaggggt
     6241 ggccacggtc gttgagaaag gaagcaagcc ggtaggtgta ctgatcaagc agggtgttta
     6301 cggttctata gagttcgtgg tagtagatcg agggcgcggt ctcgatcacc gggaggtgga
     6361 tcggcaggcc gatcacgatc accgagcgcg ccccgggaaa gatcgactgg gggtagaagt
     6421 cttccggcat ccatgggagg aacggcgggt tctcccatcg ctccgtgttc gcgaccccga
     6481 caagggggat ctccatcttc cggcactgct cgatgacggc agccttgagg tcgtcgttca
     6541 tggctatcga acacatatct ccggccgcgg aaaaaggttc atcatccacc ggcaaccggc
     6601 gggaagaggg caacctcgtc cccatccgca agggtgagag acccgatatc gttcctgttt
     6661 gcccgctttc cgttcagcat cagtatgata tgcggccgga gtatcccggt ctcatcaaag
     6721 accgcagcgg cctcgtcagc ggcacgttcc ctgaggacgg caagaaccgc ttccatctgc
     6781 gccccatccg ggagatcgat cgagaactcg ccccccagga tctccctgaa cctggcaaaa
     6841 gccttcaccc tgaccctcat atcctcaccc cgctgccgca gagagaggcg ggcggccgcg
     6901 accttctaaa catataattt caccgcaccc cgaccacgca caaaggtatt tctcccccgg
     6961 gaacagagtt aacaattgtt aatgccatgt agagtagtac tctctaacaa ttaagagttg
     7021 tggtatcatg aaacttccct gccagtcgat tgtatggaac gttctacctg caatccgtgc
     7081 cgcaatcgcg gaagagttga ttgccctcgg tgtttcccag ctggaggccg cccgcctcct
     7141 ggagatgacg cccccggcaa tctcccagta ccgcaccggt aaacggggtt accggatcgt
     7201 ctttgagggg gatgcgagag gcccggtaag ccagcttgcc cgggacctca aggacggccg
     7261 ggttgaagac cttgcatccc gcatctgtga gatctgcata cagatccgcg gagagaacct
     7321 gctcgagggc tcgtgtggca cggatgcatg agggggagtg tgccgatgga ggctgagtgc
     7381 agcagggaga tgatcatcga gaccctgaag aagcacgaac gtctgctgat cgccttctcc
     7441 ggcggcgtcg acagctctct tcttgccgca ctggccgtcc aggctctggg tcgggagcgc
     7501 gtccgctgtg ctctcctgga ttcgccgctc atccccaggc gggatgtcga agaggcgaaa
     7561 gagacagccg cggaactcgg gcttcccctg gaggttgtgg cgttccccat ccttgcagac
     7621 cccgccttcc gggaaaaccg ggctgaccgc tgttacacct gcaagaaagc ggcattccgg
     7681 ctgctcaaag aggttgccag acgggaaggg atcgagacga tcgcagacgg gacgaacctc
     7741 tctgaccttg gcgagtaccg cccggggctt gccgccagcg atgaggaggg ggtctgccac
     7801 ccccttgccg aggctggcgc aaccaaagac gatatacgga ggatcgctcg cgagtgcggt
     7861 ctcccctgct ggaacagacc ggcggcagcc tgtctagcca cccgcatccc ctacgggagt
     7921 gagataacag aagaggtgct atccatggtt gaggcggccg aggacgcgct ctgcgaccgt
     7981 ggtttctcag gggtcagggt cagggtgcac ggcagccttg ccaggatcga ggtgccggtg
     8041 gatgagatgg cacggctatt tgagatgcgc cgcgatatag cggcggagtt tcaccggatg
     8101 gggtttgtct acgtcaccct tgacctcgcc ggctaccgca gcgggagcat ggatgaggca
     8161 aatgaacatc tgcaccagcc ctgaacgcat ccggggcgca cctggaacgc ctgatcttcc
     8221 aggcagataa gattcatatc cggggccgcc aacccaccat cgatccgcca tgccctgcgc
     8281 gatcgtgaca ggtgacgtct tctcaggcca cggcgacccc ggccatcccg agtcgcaggc
     8341 acgcctcgat gccgcactga ccggtgtgcc ggcgggaaca gaccgcatca cgccggtgag
     8401 agcgacaccc ggcgacctgg ccctggtgca cacagagcgg catatcgaga ccatacggca
     8461 cctctgccgc gatctcccgc ccggccgcgc cctctacctc gacccggaca cctacatcac
     8521 cgccgggtcg ttcgacgccg ccctgtacgc ggccgggggt gcgtggcagg cggtggaacg
     8581 ggcgctcgag ggtgagcact gttttgcttt agtccgacca ccggggcatc acgccacacc
     8641 ggatcgagca atggggttct gcatcttcaa caacgtcgct atcgccgtgg aaaaggcgct
     8701 ccgcgaggtc gagcgggttg ctatagtgga ctgggatgtg caccacggga acgggacgca
     8761 ggcggcgttt tacaccacgg atcgggtttt atactgctcg gttcaccagg cagggatctt
     8821 tcccggaacg gggtggccgg aagaacgggg cgccggccgg ggtgccgggt ataacatcaa
     8881 cctgccggtt gtggcaggtt caaccggcgc cgactacgcc ctcgtattcc ggagggtatt
     8941 tgccccggca cttgcaaggt tcgaaccgga cgctctggtg gtctcggcag gacaggacgc
     9001 gctctttgat gacccgctcg cgtcgctgct cctccggcca gaagacttcg gggcgctgac
     9061 cgcgatgctt cttgaggtat acgatggggc gctcgccctc gtgctcgagg gcggctacag
     9121 cgtctcgcac ccggcggcaa tagaggcgat catcgccgct ctcgatgggg cacgcttcaa
     9181 cccggggccg ggtgagccaa aggagaccac ccggcaactt gttgaggcnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ggcgtttgag ttctaccttg gcgggagcgc
     9361 cggcccctcc ctctatgtca gactcgttgg cggccggctc ctctacgaat gggcagccaa
     9421 cggcagttat tcaggagcgg ttatggaggc agcgcccacc ccggaggaat gggcgcggtt
     9481 ccgggagacc gccgaccgcc tgggtgtcag ggagtgggag ccgcagtacc tctccgccca
     9541 ctcctgctgc gacgtcacct actggtacct caagatggag ttagacgggc aaaagatcgt
     9601 cgtgcagggc tcagacgcgt acccggcctc aaccggaccc aacgtctcaa aggagttcag
     9661 ggagtttttt cgggcggtga aaaatctggt cgggctggag ccgtgagcgt ttcatagggt
     9721 gagacgcccc aggtaatcga ggctttgctc gaacttttga aacgagaccg gttcgacaac
     9781 tttcgtggcg cccgccggga tggccgccag cacgcgacga aaattgatgt tcccctcacc
     9841 gcatgccagg tggtcatccc ggttgccgca gttgtcgtgc aggtgaacgt ggatcgctcc
     9901 ccccagttcc aggaatgttt ccaggttgcc gtttgcgtgg gcgtggccca catcgagggc
     9961 aaaacccagt ccggtatcgg caagacggcc gaggaaatcc ggttccctga agaaacagac
    10021 atcccacgtc cccatgttct cgacggcaaa ccttacgttt acctcctgct gcatcgcggc
    10081 aaggtcgtca agcgaccgat cgagggcctg ttgtgcggca ccgcgccttt cctcctccca
    10141 gacgtgcccg ggatgaatga ccagtctcac agcgccgata cggtcgcaga cctccgcgag
    10201 gtccccgatg acctggatgg ccgccttccg gaggcgctcg ttctcggatg ctatgtttat
    10261 atcggccgta ggggcgtgaa ccgtatactg caggtcaaat gaatggcagg cttcttcaga
    10321 gatgaagagg gagtgaaggc tgtcggagag tatctccgca cgatctgtga ggctggagat
    10381 cacgtccagc gcctcctcaa gtgaccggtc catcaggcac caggttgagc agccgataga
    10441 attcagtgcg gacatagaca acaggcctac cgtctctctc ttcgccacca gatcagggca
    10501 gcagcagatg caaccacagc aaccggcgtt gccggagaga gtgccttccc ctcctgttgc
    10561 gaggttgttt gcccctgatg gggtgcgccg cgggtttgag atggttctgc ggcagcggtg
    10621 acggcgttag ataaagtatc atccggtgtc ccgcatgaga tggttgctgg ttgcagcaca
    10681 aagagcgata cactcccatc agacgcaccg gttagaaatt gcatactcgc agatggagca
    10741 gagaccgacc cgataccctc ttctgttgcg tatgtccata tgttctctcc gctccggttg
    10801 aagactgcaa cacctccgtt tgccaggaga agagtcgaac cgtctctgga gatcgcaaca
    10861 tcggcgatcc tccctggcac cgaagacttc cagaggagat ttccgtttcg attcaggagg
    10921 atcacctgct ggctggatgc tgcggcaacc gtcgatccgt cgtgggagat gaccgccttg
    10981 attccgctat tacccgttct gtaagtccag aggaggcgcc catccccgct gaggaggtat
    11041 acatcacccg ccgccccgcc ggcaacgata cccgtacccc accatcgacc gatacacttt
    11101 tgatccggtc ttttgcatca aacgtccagg agacgttggc gaccgttcgc tccttgttca
    11161 gggagaagac acggaggccg gcgtcggttg ccgtgcagat ggtccgtcca tcagcggtga
    11221 gagcaacgtc ccgaatacgg gaatccggtt tataacgcca tatgatgttc ccttcccggt
    11281 caaacagaag gaggtggtct ccggcggcag cgatgcgttc gctgtcggat gagacggcca
    11341 cacccttacc gtgggacacc gggtaacgcc agagaaccgt agcgttctgg tcatagacat
    11401 agatattccg ttccgtgatg acaggagagt atagaccatc ctctgagagc gcaaccgcca
    11461 taaccgggtc tccaaccccg gaccgccaga cctcgctgcc agagatctcc tgccctgtga
    11521 ccgggcagat gagactgccg agcgcaagaa cagcaaaaag caaggttagg ggtcgactct
    11581 gccgcacgct gcatcacttc ctgcctcttt catggatcag gagaagaaca aggagcgcac
    11641 aggcaagagt gatcacgttg aaacccggcg accgggccgc ggtggtctct ttcgggctgc
    11701 ctgcctcaaa taggtcaggg tggaagatgg ccgccgcctc ttccagcgcg tccacgatcc
    11761 gcggcccccc acgactggcg gcgtcagcat cgatgacata gacgcggcc
//
LOCUS       DAOSZX010000041        11576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4744_length_11576_cov_21.061800, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000041 DAOSZX010000000
VERSION     DAOSZX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4744_length_11576_cov_21.061800"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..755)
                     /gene="nfi"
                     /locus_tag="PK196_04865"
     CDS             complement(51..755)
                     /gene="nfi"
                     /locus_tag="PK196_04865"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866233.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HON40783.1"
                     /translation="MKIKEIHTFDVEPARAIQIQETLRGHLILSGDPGEVSLVAGADA
                     SCTKGSNEIHAAVVVLGCPDFGIVERVHLSGAIGFPYIPGLLAFREGPALIEAFRRLR
                     SEPDVIFFDGHGIAHPRGIGIASHMGLLLDRPSIGVAKSLLTGSAAKPGFMRGSMTPV
                     LRDGETIGMAVRTKEGTKPVYVSVGHRISLEAAVDLVLATVRGYRLPEPTRQAHLLSN
                     AVRKESRGATRSPSVG"
     gene            complement(864..1868)
                     /gene="fen"
                     /locus_tag="PK196_04870"
     CDS             complement(864..1868)
                     /gene="fen"
                     /locus_tag="PK196_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HON40784.1"
                     /translation="MGVAIRDILVDCREPVSWDDLSGIAAVDAHNALYQFLTIIRQPD
                     GTPLMNGKGRVTSHLSGILFRTANFLEKGVRPVFVFDGKPPELKQETINERRKHRAMA
                     DEAWRLALQEGDLEEAYRQASASARIDTYILESSRELLDLLGIPWLEAPSEGEAQAAC
                     IVRKGSASYVVSQDYDSLLFGAPVLVRNLTVSGRRKVRGRMVTVNPERIILSSLLNRL
                     GLTREQLVEIGILAGTDFNPGVRGIGAKKALKIVQKGEFESVIAEKQPDFDPAPIMDF
                     FLNPPVTDDYTLEWRAPDIEGVVEMLCGRYDFSEERVRGALERFAVKTTQKTLDSWFQ
                     "
     gene            complement(1917..2231)
                     /locus_tag="PK196_04875"
     CDS             complement(1917..2231)
                     /locus_tag="PK196_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40785.1"
                     /translation="MEIRELIVDIVLTLVMVVSTLVLVMRFWQDLIIAVAATLMMLSL
                     GGLFISLGIKILNLEQSIVQRERTMRVNMEELGRTMTARYDNTISHIEEIVSDLSRRM
                     YR"
     gene            2289..3194
                     /locus_tag="PK196_04880"
     CDS             2289..3194
                     /locus_tag="PK196_04880"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HON40786.1"
                     /translation="MLIRNWLPFLGMPLMLLFVQIVAIILVMPMQAVGLVAFEDPESV
                     ANPLIFIGLLLVFTVILLILIRLGGRRIIAVFIGFALFMTFVYIFGALFSLTHGATAA
                     TAAAALIGAGAATALLYLYPEWYVIDTLGVLISAGIASIFGISLAILPVLVLLVLLAV
                     YDAISVYRTKHMITLAEGVLETRAPIMVVVPKRKDYSFRKEGLQIAEGEERGAFVMGM
                     GDLIMPSILVVSSHVFVDAPPAIWVLSAPTLGAMIGSLAGFAVLLYFVNRGNPQAGLP
                     PLNGGAIAGFLIGAALAGSFGWLSL"
     gene            complement(3133..4113)
                     /locus_tag="PK196_04885"
     CDS             complement(3133..4113)
                     /locus_tag="PK196_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HON40787.1"
                     /translation="MEFETIPTVPTADEVLDRSLRRAAARKKLKTNVDTANEEFVRAV
                     GSAIHDKLKSVVTSFPSFERLPPFYQEMTDILVSVDRLKHSLGAVAWAADQVRVISSG
                     YARSMRKSGETDRKRRQAVARMASVVHQVGDDLLFLNEARNILRKLPHVSEDEFTVVV
                     AGFPNVGKSSFIRLVSTAEPEIAAYPFTTKGVIVGHRDIGGRKRIQFIDTPGLLERPA
                     EERNPIERQAVSAIINTADLVIFILDASEHCGYLLDDQICLLKEVRGAVDVPVVPVVN
                     KADIRGIDGYPVMSTLTGEGVNEVLDLVLTLRAKATQTSLRGPLQSETRQ"
     gene            4162..5043
                     /locus_tag="PK196_04890"
     CDS             4162..5043
                     /locus_tag="PK196_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON40788.1"
                     /translation="MELSPIQKDILITLITLYHQSSHSIKGEEIAEVLRRNPGTVRNQ
                     MQALRALGLVDGVPGPKGGYSPTARAYTELNLGDLEHQFEVPIVRDGEKVKGVRVTEL
                     GFTTLCHPDLCQAVVKIIGSVKLFNMGDMVTIGPTPVNKLLVRGEVFGMDENQEALLI
                     SVSEMISLPKRPISHYMSTPVRTLPITATFRDAIHLFNKHHIHGAPVLDDAGNLAGIV
                     TLSDIARGLDTGSTLDAPIAQVMTTDVVIAPPSIRLYELVGRFKEREIGRLVVVENGK
                     PVGIVTQSDIISVFPSF"
     gene            5146..5397
                     /locus_tag="PK196_04895"
     CDS             5146..5397
                     /locus_tag="PK196_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HON40789.1"
                     /translation="MHTSEITRERRERIMTEIPLAPVGRLIKKAGAERVSSDASEELA
                     RLMEDYAARVAKEAIKLAGHAGRKTVKAIDIRMATETLK"
     gene            5618..5875
                     /locus_tag="PK196_04900"
     CDS             5618..5875
                     /locus_tag="PK196_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HON40790.1"
                     /translation="MSKTFCRSLARKKVMSNDGMLIGTIKNIMVDLSTGQVVDLLVKP
                     DETFRTDGYRTDGDKMLVPFEAVKDIKDYVVVDRYLLKRSE"
     gene            complement(5851..6555)
                     /locus_tag="PK196_04905"
     CDS             complement(5851..6555)
                     /locus_tag="PK196_04905"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HON40791.1"
                     /translation="MLRAVATDVDGTITDLRRRINTAAVEAIRTLVDTGITVVLASGN
                     TVCFMDSLSKMVGTDGTIIGENGGVYRRGFSGPLQIVGDRQVCLEAFEILRDYFARKG
                     VELEQYSAEYRFADVAFARNIDPAEAQAVIQVQGLDVRVLDTGFAIHLQPRGVSKGAA
                     MRELAVEMGIPTSEILAIGDSENDIEMLEAAGVGVAVANATPATRMAADVVTSRPHGD
                     GFVEAVKMHYSDLFSR"
     gene            complement(6549..7523)
                     /gene="radA"
                     /locus_tag="PK196_04910"
     CDS             complement(6549..7523)
                     /gene="radA"
                     /locus_tag="PK196_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HON40792.1"
                     /translation="MSEIDLEDLPGVGTTTAEKLREAGYGTVESVATATSGDLAEAAE
                     IGEATAKKIILAARKMADIGGFKTGRDIMDKRREIKKLKILVPELDELIGGGLETQAI
                     TEVYGEFGSGKSQLVHQLAVNVQLPEELGGLHGGVIYVDTENTFRPERIEQMVKGLPE
                     GAEIGSIEEVLERIHVARAHNSDHQMLLLDSARELANDLRKSDYPIRLFVIDSLTSLF
                     RSEYAGRGTLAPRQQKLNRHMHDLFKLIDDHNAVGLVTNQVMSNPGVLFGDPTKPIGG
                     HIVGHTATFRIYLRKGKGGKRVARLVDSPNLPEGEAAFMVEQAGLRSC"
     gene            complement(7631..8914)
                     /locus_tag="PK196_04915"
     CDS             complement(7631..8914)
                     /locus_tag="PK196_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HON40793.1"
                     /translation="MKFHYRLVDDLLTEDEFERRVEEKVIESGDLLDEQTAAMLVVRD
                     LGRAHIRIRDLTAVTSLACFFAKVLSIGEPREFERPDGTKGLVASVMVGDETGRARLT
                     LWDEKAAAVSELEAGAVVEILGRPKGGGTTPDVTVLAIQEAACEITCEDTPDDPGTTV
                     AGVLEVRLISVGEPRTFTRRNGSPGEMLEAVVGNEKGVFRLVAWAPALLCGVEPGESI
                     VIKGALARDNDWGVEYSVGETASVAPSGRQINITMNRVAEVEEGGSYSLSGTVVSVQH
                     ARTFTTRSGRPSKVRNLVFADETGEIPVVIWGEKADERLVPGDRIEVYNAVGRRARSG
                     GIELHLSWGSALLILEHEEEIVEIEGTVIPAGEGISIEAGNSCYLLERVIPIGYHVRA
                     RGTVQRGVFRPDFLEYMQPDLGSLKRRLDRFTPQI"
     gene            complement(8931..10223)
                     /gene="lysA"
                     /locus_tag="PK196_04920"
     CDS             complement(8931..10223)
                     /gene="lysA"
                     /locus_tag="PK196_04920"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HON40794.1"
                     /translation="MILPPHLMVKGGHLWIGGQDTQDLAQRFGTPLYVTDEDRIRENF
                     RRLAGALTAHYPNVRILYAAKANGNLAVFRILASMGAGADVFSAGEVSLALAAGMRPE
                     SLLFNGSSKSPADLALAVEKGIKVSVDSLDELRQLNAAAAEAGKTVEIAFRVNPEIDV
                     PTHQKIATGLATSKFGIPAGQILDAYREALDAGHVNPVGIHCHIGSQILAVEPFAREV
                     EVLIRLASDLIDIGADLSFIDIGGGLGIPYRRETDRAPTPEDYAGAIMPIFLKGIRDL
                     GIEPELWIEPGRWLVGDSTILLTRVNSVKTAHKTFANVDAGFNLLIRPAMYDAYHEVV
                     AANKADTPPQREYSVAGPICETGDILARDRMLPDLEAGDLIAVLDAGAYGFAMSSQYN
                     SRPRCAEVLVAGGRAALMRRAETIDDLTATMLTPPWQE"
     gene            complement(10220..11368)
                     /locus_tag="PK196_04925"
     CDS             complement(10220..11368)
                     /locus_tag="PK196_04925"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HON40795.1"
                     /translation="MYSRRMDHLPPYLFARIDEMKEEKQRQGVDIIDLGVGDPDLPTP
                     KHIVEALCAAARDPKNHHYPSYAGMPAYRRAVAEWYQRRFGVDLEPMKETLALIGSKE
                     GIAHIAEAFVNPGEVVLVADPGYPVYKTSTLFAEGKVYEMPLSPENNFLPVLEDIPAD
                     VVKQARMIFINYPNNPTAAVAPLSFFEEVVEFAREHDIVVVHDNAYSEIAFDGYRSPS
                     FLEADGAIEVGIEMHSLSKTYNMTGWRIGMACGNADIVAGLGRVKTNVDSGAFDAIQH
                     AAIAALTGPQDCVREACSIYQERRDALFSGLREIGFDVQVPKATFYVWVPVDDCMAFA
                     AKLLDEAGIVATPGIGFGRNGEGFVRFAITRSVERINEAVERMRGLDL"
BASE COUNT         2413 a         3466 c         3270 g         2427 t
ORIGIN      
        1 caccggtcgg caaacctttc tccggcgcga atgctgataa cagtttcgct ctaacccaca
       61 gatggtgaac gggtggctcc tctcgactcc ttccggacgg cgtttgagag aagatgagcc
      121 tgccgggtcg gttcggggag cctgtagccg cggacggtcg cgagaaccag gtccaccgcc
      181 gcctcaagcg agatccggtg gcccactgat acgtagacgg gttttgtccc ctctttcgtt
      241 cgcaccgcca tcccaatcgt ctcaccgtca cggagtactg gggtcatcga acctctcata
      301 aatccaggtt ttgccgcaga acctgtaagc aggctctttg ccacccctat cgagggccga
      361 tcgagcagca gccccatatg gcttgcgatg ccgatgccgc gggggtgggc gatcccatga
      421 ccgtcgaaga agatgacatc cggctcggaa cgaagccgtc tgaacgcctc gatgagagcc
      481 ggcccctccc gaaaggccag gagaccgggg atgtagggaa acccgatcgc gccgctcaga
      541 tgaacccgct cgacaatgcc aaaatcgggg catcccagga cgacaacagc ggcatggatc
      601 tcattcgagc ccttcgtgca ggaggcgtca gcaccggcaa caagcgagac ctctcccggg
      661 tcgccagaga ggatcagatg gcccctgagg gtctcctgga tctggatcgc cctggccggt
      721 tcaacatcga aggtatggat ctcttttatc ttcatgaaga tcacgagaca cctggacgtt
      781 gttgcgtggt tatgagggga gtctatttat cctgcgaaga gggggccgag cgggaagacc
      841 ccggccaaaa ggggcggggc agttcactgg aaccaggaat caagcgtctt ctgggtggtc
      901 ttaacagcga acctctcaag tgcacccctg acccgctcct cggagaagtc atagcgaccg
      961 cagagcatct caacaacacc ctcgatatca ggggctctcc actcaagggt gtagtcatcg
     1021 gtgaccggcg ggttgaggaa aaagtccatg ataggggctg gatcaaaatc gggctgcttc
     1081 tcggctataa ccgactcaaa ctcccccttc tgaacaattt tcagggcttt tttggcacct
     1141 atgccacgga ccccggggtt gaagtccgtt cctgccagga tgccgatctc gacaagttgc
     1201 tccctggtga gacccaggcg gttgaggagg gatgagagga ttatcctctc ggggttaacc
     1261 gtgaccatcc gtcccctgac cttccgcctg ccgcttacag tgaggttgcg caccagcacc
     1321 ggggcgccaa agaggagcga gtcgtagtcc tgggacacca cgtaggaggc gcttcccttc
     1381 cgtacaatgc aggccgcctg cgcctcaccc tcgctcggcg cctcaagcca ggggatgccg
     1441 agcaggtcga ggagttcgcg ggaagactca agtatatagg tatcgatacg ggcggaggcg
     1501 cttgcctgac ggtaggcctc ctccagatca ccctcctgga gcgccagcct ccaggcttca
     1561 tcagccatcg cccggtgttt ccgccgttcg ttgatcgtct cctgctttaa ctccggcggc
     1621 ttcccgtcga agacaaagac cggtcggacg cccttctcca ggaagttggc cgtccggaac
     1681 aggatcccgg agaggtgcga tgttacccta cccttaccgt tcataagcgg cgtcccgtct
     1741 ggctggcgga ttatagtgag aaactgatag agcgcgttgt gagcgtccac tgcggctatg
     1801 ccggagaggt catcccacga taccggttcc ctgcaatcca ccaggatatc acggatagca
     1861 acgcccatgc ttaccagatc atgggagtgg gagatgccat taaagtgacg atccggtcag
     1921 cggtacatcc tccgggagag gtcgctcacg atctcctcga tgtggcttat ggtattgtcg
     1981 tatctggcag tcatcgtcct gccaagctct tccatattca cacgcattgt gcgttcgcgc
     2041 tgcacaatgc tctgctcgag gttcagaatc ttgataccaa gggagatgaa aagccccccg
     2101 agagagagca tcattaacgt cgcagcaacc gctattatca ggtcctgcca gaaccgcatc
     2161 accagcacaa gggtggagac aaccatcacg agggtgagta cgatatcgac gatgagttcg
     2221 cgaatctcca tgatttatgg tgatggggat gcattaatta atctacctat aatgaatgga
     2281 gttttgtgat gctgatacgc aactggctac cctttcttgg aatgcccctg atgctccttt
     2341 ttgtccagat cgtcgccatc atccttgtca tgcccatgca ggcggtggga cttgtggcgt
     2401 ttgaagatcc ggaatcggtg gcaaaccccc tgatctttat cgggttgctg ctcgtcttca
     2461 cggtgatcct gctcatcctg atccgtctgg gtggaaggag gataatcgcc gttttcatcg
     2521 gctttgcact gtttatgaca tttgtataca tcttcggtgc gctattttcc cttacccacg
     2581 gggcaaccgc ggcaaccgct gccgcggcgc tcatcggtgc cggagcggca accgcgctcc
     2641 tctacctgta tcctgagtgg tatgttatcg ataccctggg cgttctgatc tcagccggaa
     2701 tcgcatccat cttcgggata tccctggcga tactgccggt gctcgtgctg ctcgttctgc
     2761 tcgcggtcta cgacgccatc tccgtctacc ggacaaaaca catgatcacg cttgcagagg
     2821 gcgtcctgga gaccagggcc ccgatcatgg ttgtggtgcc aaagagaaag gattactctt
     2881 tcagaaaaga gggactgcag atcgcggagg gggaggagcg cggtgcgttc gtaatgggga
     2941 tgggcgacct catcatgccc tctatcctgg tggtctcttc acacgtcttc gtcgacgcgc
     3001 ccccggccat ctgggtttta tccgcaccga cgctcggcgc gatgattgga tcgcttgccg
     3061 ggttcgctgt actcctgtac tttgtcaacc ggggcaaccc ccaggccgga cttccgccct
     3121 taaacggggg ggctattgcc gggtttctga ttggagcggc cctcgcaggc tcgtttgggt
     3181 ggctttcgct ctgagggtga gaacgaggtc gagcacctca ttcactccct ctccggtgag
     3241 ggtggacatc accgggtagc catcgatccc ccttatatcc gccttgttta cgaccggcac
     3301 gacaggcaca tcgacagccc cgcggacctc tttgagtagg caaatctgat catcgagcaa
     3361 gtagccgcag tgttcacttg cgtcaagtat gaatatgact aaatcggccg tgtttatgat
     3421 ggcactcact gcctgccgct cgatcgggtt cctctcctcg gcggggcgct ccaggagacc
     3481 cggagtatcg atgaactgaa tccgtttgcg gcctccaata tcacggtgac cgacgatgac
     3541 cccttttgtg gtgaaggggt aggcggcgat ctccggttcc gcggttgata ccagccttat
     3601 gaaagaggat ttccccacat tcgggaaccc ggcaaccacc acggtaaact catcctcgct
     3661 cacgtgcggc agtttgcgca ggatgttcct cgcctcgttg aggaagagaa gatcatctcc
     3721 aacctggtga acgaccgaag ccatccgggc aacggcctgc cgccgtttcc ggtcggtctc
     3781 tccgctcttt cgcatgctcc gggcgtagcc ggaactgata acccggacct gatcggccgc
     3841 ccaggcaaca gcacccagcg agtgtttcaa ccgatcgacc gagacgagga tatcggtcat
     3901 ctcctggtag aatggcggga gacgctcaaa actcgggaac gaggtgacca cgctctttaa
     3961 tttatcgtgt atagcgctcc cgaccgcccg cacaaactcc tcgtttgctg tatcgacgtt
     4021 ggtcttcagt ttctttctgg ctgccgccct ccgaagactg cggtcgagaa cttcgtcagc
     4081 cgtcggaacg gtcgggatgg tttcaaattc cacgctacac acaccctaat gtatttaaac
     4141 aacatataat gactgataac tatggagctc tcccctattc agaaggatat tctgataacc
     4201 ttaatcaccc tttaccatca gtcttcccac tctataaaag gtgaggagat cgcagaggta
     4261 ctgaggcgta atcccggcac cgtccgcaac cagatgcagg cgttgagggc actcggactg
     4321 gtcgatgggg tgccgggacc aaaggggggt tacagcccaa ccgccagagc atatacggag
     4381 ttgaatctag gggatctcga gcaccagttc gaggtaccga tcgtgcgcga cggtgaaaag
     4441 gtaaaaggcg tccgcgtcac cgaactcggg tttaccaccc tctgccaccc tgacctctgc
     4501 caggcagtgg tcaagatcat cggaagcgtt aaactcttta acatgggaga tatggtcact
     4561 ataggcccaa cccccgtgaa taaactcctc gttcgaggcg aagtgttcgg gatggacgag
     4621 aaccaggagg cgctcctgat cagcgtctca gagatgatct ccctcccaaa acgtccgatc
     4681 tcgcattaca tgagcacacc ggttcggacg ctgccgatca ccgcaacctt ccgcgatgca
     4741 attcacctct tcaataaaca ccacatacat ggtgcaccgg tccttgatga tgcgggaaac
     4801 cttgcaggca ttgtgacatt gagcgatatc gcccggggac ttgacaccgg gtcaacactg
     4861 gatgcgccca tcgcgcaggt catgacaacc gacgtggtga ttgcaccacc atctatccgg
     4921 ctctacgaac tcgtgggccg gttcaaggaa cgggagatcg ggaggctggt tgtggtcgaa
     4981 aacggcaaac cggtcgggat cgtcactcag agcgacataa tcagcgtctt cccctcgttt
     5041 tgatccctct tttgcgaaac caactgcacc aacccctcca gtgagggaca agtatttata
     5101 ggtggctatc aatggtcttt ctgttaagag atttctgatc agaggatgca tacctcagag
     5161 attactagag aaaggagaga aaggatcatg actgaaatac ctcttgcacc tgttggaagg
     5221 ctcataaaga aagccggtgc ggaacgggtg agttctgatg ccagcgagga actcgcaaga
     5281 ctgatggaag attacgctgc gcgggtcgca aaagaggcga tcaaactggc gggtcatgca
     5341 ggcaggaaga cggtcaaggc aatcgatatc cggatggcga ccgagactct gaagtgaacc
     5401 atccaatttt tgactgactg tcttttcccc gccagaggaa aactccaacg gtctttaacc
     5461 cggggcaggg gcacggttag cgtggcatcc gccagaataa tggccgactg tcaacccctg
     5521 aaggaagggg tcagtctgag ggttttctac gtaaagcggg ttttaggggg tttcacccgc
     5581 ttataagatt tatatattac aaaaccacat gacttgtatg agcaaaacgt tctgccgctc
     5641 ccttgccagg aagaaagtga tgagcaacga tggcatgctc atcggaacca tcaagaatat
     5701 catggttgac ctgagcaccg ggcaggttgt cgaccttctt gtcaagccgg acgagacgtt
     5761 caggaccgat ggttacagaa cggatggaga caagatgctc gtgccgttcg aggctgtaaa
     5821 agatataaag gattacgttg ttgtagaccg ctacctgctg aagagatctg agtagtgcat
     5881 ttttaccgcc tccacgaagc cgtcaccgtg tggccttgag gtgacaacgt ccgcagccat
     5941 gcgtgtggcc ggggtggcgt ttgcaaccgc tacccctaca ccggcggcct cgagcatctc
     6001 gatgtcgttc tcggagtccc cgatagcgag aatctctgag gtggggatac ccatctcaac
     6061 ggcgagttca cgcatcgccg cccccttgct cacgccccgt ggctggaggt ggatcgcgaa
     6121 tccggtatca agcacgcgta catctaggcc ctgcacctgg atgaccgcct gcgcctcagc
     6181 gggatcgatg ttccgcgcga aggcgacgtc agcaaaccgg tattctgcac tgtactgctc
     6241 gagttcaaca ccctttctgg caaagtaatc cctgagaatc tcaaacgctt ccagacagac
     6301 ctgccggtca ccgacgatct ggagagggcc ggagaaacct ctccggtaga caccaccgtt
     6361 ctcaccgatg atagtcccat ccgtcccgac catctttgag agactatcca tgaaacagac
     6421 ggtgttgccg cttgcaagca caaccgtgat cccggtgtcg acgagggtgc ggatggcttc
     6481 gacggcggcg gtgttgatcc gccgccgcag atcggtgatg gtgccatcga cgtcggttgc
     6541 aaccgctctt aacacgatct gagccccgcc tgctcgacca tgaacgctgc ctcgccttca
     6601 ggcagattgg gactatccac caggcgggcg accctcttgc cacccttgcc tttccgcaga
     6661 tatatccgga atgttgcggt gtgcccgacg atgtggccgc cgatcggttt tgtcggatcg
     6721 ccgaagagaa cgccagggtt cgacatcacc tggttggtca caaggccaac agcattgtgg
     6781 tcgtcgatca gtttgaacag gtcatgcatg tggcggttca gtttctgctg cctgggtgcg
     6841 agcgtgccgc gccccgcata ctccgaccga aagagcgagg tcagggagtc gatgacaaag
     6901 agcctgatgg ggtagtcgga ctttcggagg tcgtttgcaa gttcacgtgc gctatcaagg
     6961 aggagcatct ggtggtcgga attgtgcgcc cgcgcgacgt ggatccgctc caggacctcc
     7021 tcgatagaac cgatctccgc cccctcaggc aggcccttaa ccatctgctc aatacgctcc
     7081 gggcggaagg tgttctcggt atcgacgtag atcacaccac cgtgcagccc cccgagttct
     7141 tcagggagct ggacgttgac cgccagctga tggacaagct ggctcttgcc ggaaccgaac
     7201 tcaccgtaaa cctcagttat cgcctgcgtc tcgagcccgc cgccgatcaa ctcgtcaagt
     7261 tcgggcacca ggatcttgag tttcttgatc tcccttctct tgtccatgat atcccggcca
     7321 gtcttgaacc ccccgatatc cgccatcttc ctggcggcaa ggattatctt cttggcggtc
     7381 gcctcgccga tctccgccgc ttcggcgaga tccccagacg ttgctgtcgc gacgctctct
     7441 actgtaccgt atccagcttc acggagtttc tcggcggtgg ttgtcccaac tccgggtaaa
     7501 tcttcaagat caatctctga catctcgatc ctcacttgat ctcttatgtg tgtccaatga
     7561 cgctacacta agactctttg actgtagaga taaaattcga ttgccgcgcg gtgtgaaagt
     7621 gaagcctgga tcagatctgg ggggtaaacc gatccaggcg gcgtttcagc gatccgagat
     7681 cgggttgcat atactccaga aagtccggcc tgaacacgcc acgctgaacc gtgcccctgg
     7741 cgcgcacgtg atagccgatg gggatcaccc tctccaggag gtagcaggaa ttgcccgcct
     7801 cgatggagat cccctcgccg gcagggatga ccgtgccctc gatctcaacg atctcttcct
     7861 catgctcaag gatgaggaga gcgcttcccc acgagagatg caactcgatg ccgccagagc
     7921 gcgcccgccg ccccaccgcg ttgtagacct cgatccgatc gccgggcacc aggcgttcat
     7981 cagccttctc gccccagatt acaaccggga tctcacccgt ctcatcagca aagacaaggt
     8041 ttcgaacctt tgatggccgc ccgctccgtg tggtaaacgt ccgggcatgc tgcacactga
     8101 ccacagttcc agagagcgag tacgacccgc cttcctccac ctccgcaact ctgttcatag
     8161 tgatgttgat ctgacggccg gagggtgcaa ctgatgcggt ttcaccgacg ctgtactcga
     8221 ctccccagtc attgtcgcgt gcgagagcgc ccttgataac gatgctctcc ccaggctcaa
     8281 ccccacagag gagcgccggc gcccaggcga caagtcggaa cacgcccttc tcgttcccga
     8341 ccaccgcctc gagcatctcc ccgggagatc cgttgcgccg ggtgaacgtc cgcggctccc
     8401 ctaccgagat cagcctgacc tcaagaacac ccgcgactgt tgttccgggg tcatcagggg
     8461 tatcctcaca agttatctcg catgccgcct cctggatggc gagaacggtg acgtcaggag
     8521 tcgttccacc gcccttcggg cgcccgagta tctctacgac ggcacctgcc tccagctcag
     8581 agactgcggc ggccttctcg tcccagagtg tcaaccgggc cctcccggtc tcatcgccca
     8641 ccatcacgct cgctacaagc cctttcgtac cgtcaggccg ctcaaactcc cggggttccc
     8701 cgatagagag cacctttgca aagaagcacg cgaggcttgt gaccgccgtc aggtcgcgta
     8761 tacggatgtg cgcccggcca aggtcacgga cgaccagcat agccgccgtt tgttcatcga
     8821 gaagatcgcc tgactctatc accttctcct caacccggcg ttcaaactca tcttccgtga
     8881 gaaggtcatc gaccagacgg taatggaact tcacgcccgc atctccgctc ctactcctgc
     8941 catggcgggg tcaacatcgt ggcggtcagg tcgtcgatcg tctcggcccg acgcatcaat
     9001 gcagcccttc cacctgcgac cagcacctca gcgcagcgtg gccgactgtt atactgcgat
     9061 gacatcgcaa acccgtatgc cccggcatcc aggacggcga tgagatcacc ggcctcaagg
     9121 tcagggagca tacggtccct ggcaaggatg tcacccgtct cacagattgg ccccgcgacc
     9181 gagtattccc gctggggggg agtgtctgct ttgtttgctg ctactacctc gtggtaggcg
     9241 tcgtacatcg ctggacggat gagcaggttg aacccggcat cgacgtttgc aaaggtctta
     9301 tgagcggtct tgactgagtt gaccctggtg aggagaatcg tcgagtcgcc gacgagccag
     9361 cggcccggtt caatccagag ttctggctcg atcccgaggt cacgtatccc cttcaggaag
     9421 atgggcatga tcgctcccgc atagtcctcg ggcgtcgggg cacggtcagt ctcccggcgg
     9481 taggggatgc cgagccctcc cccgatgtca atgaaggaga ggtctgcccc gatatcgata
     9541 aggtcgctcg cgagcctgat cagcacctcg acctcgcgcg cgaatggctc cacggccagg
     9601 atctgcgagc cgatatggca gtggataccg acagggttga catgcccggc atcaagggcc
     9661 tcccggtagg catccaggat ctgtcctgca ggaatgccga acttgcttgt cgcaagcccg
     9721 gttgcgatct tttggtgggt aggaacatcg atctcagggt tgaccctgaa agcaatctcg
     9781 accgtctttc ccgcctctgc ggccgccgca ttgagttgcc ggagttcgtc aagcgagtcc
     9841 accgagacct ttatcccctt ctcaaccgcg agggcaaggt cagcagggga tttcgagctc
     9901 ccgttgaaga ggagcgactc cggccgcatc ccggcggcaa gggcgagaga gacctcccct
     9961 gcggagaaca catcggcacc cgcgcccatc gaggcaagta tcctgaagac cgcgagattg
    10021 ccgtttgcct tggcggcgta gaggatccgg acgttcggat aatgcgcggt gagggcgcct
    10081 gcaaggcgcc tgaagttctc ccggatccgg tcctcgtcgg tgacgtagag cggagtgcca
    10141 aaccgttgcg cgagatcctg ggtatcctgg ccgccgatcc agagatgacc gcccttaacc
    10201 atgaggtgcg gcggaaggat cacaggtcaa gcccccgcat ccgctcgacc gcctcgttga
    10261 tccgctcgac cgaccgggtg atcgcgaaac ggacgaaccc ctcgccgttt cgaccgaaac
    10321 cgatccccgg ggtcgcgaca atcccggcct catcgaggag tttcgcggca aatgccatgc
    10381 agtcgtcgac cggcacccat acgtagaagg tggccttcgg cacctgaacg tcaaacccga
    10441 tctctcgaag acccgagaag agcgcgtccc ggcgctcctg gtatatcgag caagcctctc
    10501 taacacagtc ctgagggcct gttaatgcgg cgatcgcggc gtgctggatg gcatcgaacg
    10561 ccccggagtc gacgttggtc ttgacccggc caagccctgc tacaatatcg gcgttcccgc
    10621 aggccatccc gatccgccac ccggtcatgt tgtaggtctt tgagagggag tgcatctcga
    10681 taccaacctc aatcgcaccg tcggcctcga ggaatgaggg cgagcggtag ccatcgaagg
    10741 cgatctcgga gtaggcgttg tcatggacca cgacgatatc gtgctcccgt gcaaactcaa
    10801 caacctcctc aaagaatgag agtggcgcca ctgctgccgt ggggttgttc gggtagttga
    10861 tgaagatcat cctggcctgc ttaacaacat cggcggggat atcctcaagc accgggagga
    10921 aattgttctc ggggctgagc ggcatctcat agaccttgcc ctcggcaaag agcgtggagg
    10981 tcttgtagac ggggtaaccg ggatctgcga ccaggacaac ctcaccgggg ttgacaaagg
    11041 cctcggcgat gtgcgcgatc ccctcttttg aaccgattaa cgcgagggtc tccttcatcg
    11101 gctcgaggtc aaccccgaac cgacgctgat accattctgc caccgccctg cggtatgcgg
    11161 gcataccggc ataggagggg tagtgatgat tcttagggtc acgggcggcg gcgcagaggg
    11221 cctccacgat atgcttaggc gtggggagat cgggatcgcc gaccccgagg tcgattatat
    11281 cgacgccctg acgctgcttc tcctctttca tctcgtcaat gcgcgcaaaa aggtagggag
    11341 ggaggtgatc catgcgtctc gaatacatca ttcaggtatt gcacacgaaa ccctatttat
    11401 cttctcacct tacgggaaac tcatgcccgg acaacatgat cggaaaaagg gataccgttt
    11461 acgctctggc gcgcagtgga cgaaaaagga gtatcgccgc ccgggaggcg acggtgcggc
    11521 gacagtctga ggctatccgt accaatccct tcaggctgtg cccccatctg attatc
//
LOCUS       DAOSZX010000042        11435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4823_length_11435_cov_19.669069, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000042 DAOSZX010000000
VERSION     DAOSZX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4823_length_11435_cov_19.669069"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="hypD"
                     /locus_tag="PK196_04930"
     CDS             <1..798
                     /gene="hypD"
                     /locus_tag="PK196_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HON40796.1"
                     /translation="YGDLLRVPGSSGSLESSGGDVRVVQGVHKAVEIARREPEREVVF
                     ISVGFETTAPTVAATILSHPPENFSILSCHRLVPPAMAWLLDQGEAALDGFILPGHVC
                     TVMGYQEYERFPVPQVVAGFEPEDILLGLLMAVRQVREGRHEVENAYPRAVTREGNTK
                     AKRLMYEVFEPVDLEWRGFPVIPASGLRLKPEFEGYDAQKKFDIEIRHVEKHSACICD
                     RVLRGIARPADCKLFGKACTPRTPVGPCMVSHEGACRIWHLYESRRG"
     gene            852..923
                     /locus_tag="PK196_04935"
     tRNA            852..923
                     /locus_tag="PK196_04935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:885..887,aa:Arg,seq:ccg)
     gene            complement(966..2108)
                     /locus_tag="PK196_04940"
     CDS             complement(966..2108)
                     /locus_tag="PK196_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON40797.1"
                     /translation="MSEHSDHRTEREALREKIIGLGEASLHKSYYPELQARLAELERF
                     RALLDQTYDAIFLIRAKGGRIVDVNATAEVYLGYSRGELLSRSFDDLVIPEQRSGFLA
                     YTRRKHRSPAESEETYRVSLLTRDSREIPVEVAFRSVTFGDDRYIVAIARDITERIRV
                     ERDLRIKESAIESSINGILIADPEGVVTYANPSFAEMFGYSGPEALRGMNLKRFFVDP
                     SAAEEIVRDLLENRPVILETEGLRSDASPFYIEVSGSVVRNDAGDPICIMLVVMDITE
                     RIRLDRLKRESYAQLGKNIEQFAVIGDHIRNPLQVIVGCAEMIDDPLAEKILEQARLI
                     DRFIAELDRGWIESLNVRNFLRKHGEGVELPERRPISLSSQTDEDS"
     gene            complement(2092..3240)
                     /locus_tag="PK196_04945"
     CDS             complement(2092..3240)
                     /locus_tag="PK196_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HON40798.1"
                     /translation="MNQRNRLELRKFVAPEFVCGQGALRLAGRYAHNLGMRRVLLVTD
                     AGVRAAGWADAVGESLASAGASFTIFDSVSPNPRAGEVMAGAEVYRSEDCNGIVAVGG
                     GSPMDCAKGIGVVSVSGRDILAFEGVDRVSVPPPPLVCVPTTAGTSADLSQFAIIRDE
                     MGRRKVAIISKALVPDISLLDPEPLTTMPRELTVDTGIDALSHAIEAYVSNASSPITD
                     LHALEAVRLTSTALPEVIESPDDLNLRFSTLLASLHAGLAFSNASLGAVHAMSHALGG
                     VYDLAHGRCNALLLEDVIEANYEAASIRYDRIGAIIGRGQDEWMAPAEVAASFIRSLG
                     VTGTLSSHGVTRDMIPALAERAYVDPCLATNPRDLSREEIEEIYERAL"
     gene            3741..4403
                     /locus_tag="PK196_04950"
     CDS             3741..4403
                     /locus_tag="PK196_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HON40799.1"
                     /translation="MIFSLLACCFIVLPSSAQPPIGGDNGWYAVNCNVNGADVYFDSE
                     YKGTIEAGVLYVPVYTTGAPYQTFRVEKDGYTTYYGEVTSVPGKGEVFDLYATLNPVQ
                     PTAAPIIGGDIGWYTVHVNVDGATVQFDNEVKGVTSQGVLSVEVYTTGTPYRTYTVTK
                     EGYQTYTGTIDRYPGKGETVDLYVTLNPITTPTQTAPLSPAVAGGALLIAGLLLAAGR
                     KN"
     gene            4910..5632
                     /locus_tag="PK196_04955"
     CDS             4910..5632
                     /locus_tag="PK196_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40800.1"
                     /translation="MTSTESYSCPNCGAVFTREGSNRFKLSNISDTTRPAWRDYANHT
                     LSDLEWKRIAGGGVSDARQRELDLDHVITAIVEGQIQALNAAATGSIVPKRGERLVVT
                     VPGVTLSEPRSVRETHGGGGGPSFRVAKGVYIRTGAFASRSESHEEIRSIDSGTLSLT
                     DRRLVFAGQKRTKSINLGKIVSIKPFKDGISINREGKQKTEYYSGLDNAILRFSIEGR
                     DYEEPLSGAILARLIEGLLKTD"
     gene            complement(5641..5940)
                     /locus_tag="PK196_04960"
     CDS             complement(5641..5940)
                     /locus_tag="PK196_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40801.1"
                     /translation="MERSNSGADRCSAGAPGPRVDRKALLEEIAAFTHKYGDILARYH
                     RYTMDELKQIEQECWALHDKACSMGACGSAGVLAELEYLITTAKEMRARMDESRG"
     gene            complement(6151..6987)
                     /locus_tag="PK196_04965"
     CDS             complement(6151..6987)
                     /locus_tag="PK196_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON40802.1"
                     /translation="MVDSDYYKDRVCIVTGANSGIGYAISEELLKRGATVYMAGRSPE
                     KIAKAVGQLTAYGDRVKPLIMDVTVQEQVQRGIEATAEKAGKLDILFNNAGVGGTMPF
                     ERATLEDWKTIIDTNLWSVIYGVHTAVPIMLKQGFGHIVNTSSIAGIAPPPFQALYSL
                     TKYGVTGLTECLRYEYADRGLYFSTICPANIATPIFNKGIDGQAHGELRIPDDAYPVE
                     RAASLILDRVAEHKGIIVVPEHPYTELWQGYILGRPEIEETLLQMARDRREAFERGGT
                     YF"
     gene            complement(7003..7797)
                     /locus_tag="PK196_04970"
     CDS             complement(7003..7797)
                     /locus_tag="PK196_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HON40803.1"
                     /translation="MFRTHDDFTYLMPVHFGGSRFDPETLVTQRAASLAISYETGRDL
                     LENYIPEGFELLAPEVQVVFSRFTEINWLQGGHYNLINVAAPVRFHGKKDEIDGAYTL
                     VVWENRTAPILGGREQTGIPKIHADIDDLHILRPHYATTASYEGNTFLRIGFEATGPI
                     TGPDLEALKSQFVSMNTLGWKYIPKVGAPGAELSQFVLYPQGMEVETAQAGRGSLEWI
                     EQTPMQNPVQYYIINSLAALPMKRVTQSVMVEGRAILRAMGARVIE"
     gene            8217..8852
                     /locus_tag="PK196_04975"
     CDS             8217..8852
                     /locus_tag="PK196_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40804.1"
                     /translation="MVEETAGKERPGLKTRAESDLSLEVVILIVFGVFMLLFGVLLFR
                     IHTGELPYAPDSTYGLFLVIVSFQIITMGRTPFGDLRRSWAVILLGVFTAVIGMVACF
                     IPGLLAEPVRILVGILLSAGGVSLMLQLLFSRDKARLWLKAPGILRQLTLACILVYLF
                     SIVLGLVTLLPGITTDPQTAVLLIIYGISYFYLSWCIQNANRAYPPGERTA"
     gene            8858..9478
                     /locus_tag="PK196_04980"
     CDS             8858..9478
                     /locus_tag="PK196_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40805.1"
                     /translation="MDSSGPPLKPGLRFFRETSIPLSIALVIPVGVLLVLLGLLLFPV
                     NMGAIPFSPDGQLGLLLVIMAIQMLALGETPMGRYRRSHLLAAIGIAFASMGIFSCIV
                     PGVLTDVIRVLLAILNIAGGAILLAKRFLPISQGAGNAAAAVPASLMPTFKRLTATQT
                     VLNVVSISFGISMLLPGLIPGMVIAGIVVVNGLLLFALAAILMKVV"
     gene            complement(9537..9621)
                     /locus_tag="PK196_04985"
     tRNA            complement(9537..9621)
                     /locus_tag="PK196_04985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9583..9585),aa:Leu,seq:cag)
     gene            complement(9659..11356)
                     /locus_tag="PK196_04990"
     CDS             complement(9659..11356)
                     /locus_tag="PK196_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON40806.1"
                     /translation="MADVEQNRPSYEELCANFRIDVPEYYNFGFDVVDAWAKKDRNKL
                     AMIWVDQKGNEKKYTFFDMMRLSNQAVNICLKYGIKKGDRVMLMLPRVPEWWIFVIAL
                     IKLGAVYCPATTMLTPKDLKYRIQAAGIKMVITMAEHAEKVEEIRDECHSLEVMLMTD
                     GKRDGWVSYPVELDYPAPCSYKLVNLPGMHRTKSTDPLLIFFTSGTTGEPKMVLHDHS
                     YPLGHIVTAQLWHDLHPNDLHLTLSDTGWGKSAWGKLFGQWIVGACIFVYDIRGKFNP
                     TEILPLLEKYGVTTFCCPPTIYRMLILADLDKFDLAELRHCCSAGEALNPEVIRAWEE
                     GTGKTIYEGYGQTETVLCIGTLPGMKVKPGSMGRPMPGWHIELHDDDGKPVGPMEEGR
                     IAIRLNPRPVGLFRGYLDASEEENRRAFQNGFYYTGDKARMDEDGYFWFVGRDDDVIK
                     SSGYRIGPFEVESALMEHPAVQEAAVVGSPDVIRGMVVKAFIVLKPGYKPSEALVKDI
                     QRYVKRVTAPYKYPRRVEFVESLPKTISGKIRRHELRELEMKRFMENNSRNSSAREE"
BASE COUNT         2281 a         3458 c         3293 g         2403 t
ORIGIN      
        1 tacggcgacc tgctgcgtgt cccggggtcg agcggatcgc ttgagtcaag cggcggggat
       61 gtccgcgtgg tgcagggagt gcacaaggcg gtggagatcg ccaggcggga accggagcgt
      121 gaggtcgtct ttatatcggt aggattcgag accacagcac cgacggtcgc cgccacgatc
      181 ctctcccacc cgccggagaa cttctcgatc ctctcctgcc acaggcttgt ccctccggcg
      241 atggcctggc tccttgacca gggcgaggcg gcgcttgatg gatttatcct ccccggtcac
      301 gtctgcaccg ttatgggcta ccaggagtat gagcggttcc cagtcccgca ggtcgttgcc
      361 gggttcgagc ccgaggacat cctcctcggg ctgctgatgg cggtcaggca ggtccgcgag
      421 gggagacatg aagtggagaa cgcctacccg cgtgcggtca cgcgggaggg aaacaccaag
      481 gcaaaacgcc tgatgtatga ggtcttcgaa ccggtcgatc tcgagtggcg cgggttcccg
      541 gtgatcccgg cctccggcct ccggttgaaa ccggagtttg agggctacga cgcgcagaaa
      601 aagtttgata tcgagatcag gcatgtggag aagcactccg cctgcatctg cgaccgtgtc
      661 ctgcgcggaa tcgccaggcc agccgactgc aaactcttcg ggaaggcctg caccccccgc
      721 accccggtcg gcccatgcat ggtcagccac gaaggggcgt gcaggatatg gcatctctac
      781 gaatcgcgga gaggctgaaa ctcccactct ccgctggcct tttataccat atcaaacaac
      841 ctaatatggc agtcccggta gggtagtgga catcctgaaa gcctccggag ctttcgaccc
      901 gggttcaaat cccggccggg gcgtttactg tttttcttca gagcaggacg ggttccacct
      961 gctgctcagg agtcttcatc cgtctggctg gagaggctga tcggtcttct ctcgggcaac
     1021 tccacaccct ccccgtgttt ccgcagaaag ttcctgacat tcagggactc aatccagccc
     1081 cggtcaagtt ccgcgataaa ccggtcgatc agcctcgcct gctccaggat cttctccgca
     1141 agcgggtcgt cgatcatctc cgcgcagccg acgatcacct ggagagggtt ccggatatgg
     1201 tctccgataa cggcaaactg ctcgatattc ttcccaagct gcgcgtagga ctcacgtttc
     1261 agccggtcaa gcctgatgcg ctcggtgata tccatgacaa caagcattat gcagatagga
     1321 tccccggcat cgttccgcac gacactgccg gagacctcga tgtaaaacgg cgatgcgtcg
     1381 cttcgcaacc cctccgtctc cagtatgacc ggccggttct ccaggagatc gcggactatc
     1441 tcttccgcag cggatgggtc aacgaagaac ctcttcaggt tcattcccct gagcgcctcg
     1501 gggccgctgt agccgaacat ctctgcaaaa ctcgggtttg cgtaggtgac cactccctcg
     1561 gggtcggcga tcagtatccc gtttatggat gactcgatcg cgctctcctt gatcctgagg
     1621 tcgcgctcga cgcggatgcg ctcggtgatg tcccgtgcta tcgccacgat gtagcggtca
     1681 tcgccgaagg tgaccgaacg gaaagcaacc tccaccggga tctcccggct gtcacgagtg
     1741 agaagcgaga ccctgtaagt ctcctctgac tccgccggcg acctgtgttt tcgccgggta
     1801 taggcaagga agcctgaccg ctgctcaggg attacaaggt cgtcaaacga tctggagaga
     1861 agttctcccc tcgagtagcc cagatacacc tctgcggtgg cgttgacatc cacgatcctc
     1921 ccgccctttg cccggatcag gaagattgca tcgtaagtct ggtcaagcag cgccctgaac
     1981 cgctcaagtt cagcaagcct ggcctggagc tccgggtagt aactcttatg aagggaggcc
     2041 tcaccgaggc cgatgatctt ctcgcggagt gcttcccgct ccgtccggtg gtcagagtgc
     2101 tcgctcatag atctcctcaa tctcctcgcg tgagaggtcg cgggggtttg ttgccaggca
     2161 ggggtcaacg tatgcgcgtt ctgcaagtgc agggatcata tccctggtga cgccgtggct
     2221 ggaaagcgtc ccggtgacgc caagcgacct gatgaatgac gccgccacct ccgccggcgc
     2281 catccattca tcctgccccc gtccgatgat cgcaccaatc cggtcgtatc ggatggatgc
     2341 cgcctcatag ttggcctcga tcacgtcttc caggagaaga gcgttgcacc ggccgtgtgc
     2401 aaggtcatag accccgccaa gagcgtgcga catcgcgtga accgcaccga gactcgcatt
     2461 ggagaacgca agaccggcgt gaaggctggc aaggagggtt gagaaccgga ggttgaggtc
     2521 atcggggctc tcgatgacct caggcagagc ggtgctggta aggcgtaccg cctccagggc
     2581 gtgcaggtcg gtgatcgggg aactggcgtt tgagacgtat gcttcgatcg cgtgactgag
     2641 ggcgtctata ccagtatcca cggtgagttc ccgcggcatg gtcgtcaggg gttcggggtc
     2701 gagcagcgag atgtcgggta caagcgcctt ggagatgatg gccactttac gtctccccat
     2761 ctcgtccctg atgattgcaa actgggagag atcagcggat gtccctgcgg ttgtcgggac
     2821 gcatacgagc ggcggcggag gcacagagac ccggtcaaca ccctcgaacg cgaggatatc
     2881 ccgcccgctc acgctcacca ccccgatgcc cttggcacag tccatcgggc tccctccacc
     2941 gacagcgacg atcccgttgc agtcttcaga acgataaacc tccgcccctg ccatcacctc
     3001 accggcacgg gggttcgggc tgacagaatc aaagatggta aacgaggccc cggcgctggc
     3061 caggctctcc ccgacggcat cagcccatcc ggccgccctg accccggcgt cggtgacaag
     3121 aaggacacgc cgcatcccga ggttatgggc ataccggccg gcgaggcgca gcgccccctg
     3181 accgcagaca aattcggggg ccacgaactt tctaagttca agtcggtttc tctgattcat
     3241 ccatcacaca cgaccgggat tctaatcgtc atctggagac gttccagggg ttgcaggggg
     3301 aactgttatc cagtagtggt gggttgtgtt catatatatt ttggcgaacc cctgtcaggc
     3361 ggcaatatac gttacgatat atattcggcg gtaatccaga gccatttcag gatttttctg
     3421 ctggatcccg ggttcaggca tttgagacgc tcgatacgcg gcgatctcgc gcatccttga
     3481 gagataatca gacgcacggg gagggttgtg cgcaaaaatg cccccccctg ccgccgccac
     3541 gcctccctcg tgccggggtg tgggtccgcg catacgatca tccctaaaca gaagagagca
     3601 cggggttcac ggctgtggaa gaaccgcagc cgatctgcac aatctttact ccgtccaggg
     3661 gttggcggca ggatatctta agaaagattt tttgcctatt aaagtagagg ctctgcatgc
     3721 agagcgtttt acgcaacctg atgatctttt ctcttctcgc ctgctgtttt attgtcctgc
     3781 cctcgagcgc ccaaccgccc ataggcggag acaacggatg gtacgcggtg aactgcaacg
     3841 tgaatggagc agatgtctac ttcgacagcg agtataaggg aacgatcgag gccggtgtgc
     3901 tctatgtccc tgtctacacc acaggagcgc cctaccagac cttccgcgtg gagaaagacg
     3961 gctacacaac atactatgga gaggtcacct cagttcctgg caaaggggag gtgtttgacc
     4021 tctacgcgac cctcaacccc gtccagccca ccgcagcacc gataatcggc ggggatatcg
     4081 gctggtacac cgtccacgtc aatgttgacg gggcaacggt tcagttcgac aacgaggtca
     4141 agggtgtcac aagccagggc gtcctttctg tggaggtcta cacaaccgga acaccataca
     4201 ggacttacac cgtgacaaag gaagggtatc agacctacac cggcaccatc gaccgctacc
     4261 ccggcaaggg tgagacggtc gacctctacg tgacgctgaa cccgatcacg acgcccacac
     4321 agaccgcacc gctctctccg gccgttgccg gcggcgccct gcttattgcg gggctgctgc
     4381 tggctgcggg gagaaagaac tgaacaaact ctttttcata attttcgcgg gcaccaaact
     4441 cgaacctttt ccccggagcc gctcgtactg ccggcaaccg cacaggagcg caaaaggcga
     4501 ctctcaccgt ttccagaaca ggctcttccc ccataccgct ccggggcatc gccgatcgct
     4561 ctccaccagc atggtgagaa ccgttccggg cccgcccggg cgattggaaa agagatatac
     4621 agccgcacga ccactccctc tataacgctg ccccgcgggc gagaacgatc gcgactgcgg
     4681 aagataccgg gaacagaaaa accggtgccg ccagcgagag agggggctga tccagatgcg
     4741 atgtccaaac tgtggctatg aggatgaggg aaaatactgc agcaactgcg gcagccccct
     4801 cctgcaccaa cctgtcgatt ctgccccgga taagggggaa tggtatgacc gatgccctgc
     4861 atgcagagcc ggtcgcctct caaagaccgt cagcagggga ttcctgggtt tgacgagcac
     4921 agagtcgtat tcgtgcccga actgcggagc ggtcttcaca agggagggca gcaacagatt
     4981 caaactctcg aacatctccg ataccacaag acccgcgtgg cgcgactacg ccaaccatac
     5041 attgagcgat ctggagtgga agcgtatcgc cggtggcggg gtctccgatg ccagacaacg
     5101 ggaactcgac ctcgaccacg tcatcacagc aatcgttgag gggcagatcc aggcattgaa
     5161 cgctgctgct accggttcga tcgttccaaa gagaggcgaa aggcttgtgg tgaccgtccc
     5221 cggggtcacc ctgagcgagc cgcgctccgt gcgggagacg catggaggcg gtgggggacc
     5281 aagttttcgc gtcgcaaaag gggtttacat aagaaccggg gcttttgctt ccaggagcga
     5341 gtcgcacgag gagatacgat cgatcgattc aggcacgctc agcctgacag acagacgcct
     5401 ggtgtttgcg gggcagaaac ggacgaagag catcaacctg ggaaagatcg tctcgataaa
     5461 gcccttcaag gacgggatct ccatcaaccg cgaggggaaa cagaagacag agtactactc
     5521 gggcctggat aacgccatcc tgcggttcag catcgaaggt agggactacg aagaacccct
     5581 ctcgggcgcg atccttgccc gcctgatcga ggggctgctc aaaaccgact gaggcgcggg
     5641 tcagccgcgg gactcgtcca tcctggccct catctccttt gccgtggtga tgaggtactc
     5701 cagttcagcg agtaccccgg cgctcccaca ggcgcccatg ctgcacgcct tatcgtgcag
     5761 cgcccagcac tcctgctcga tctgtttgag ttcgtccatc gtgtagcgat gatacctggc
     5821 aaggatatcc ccgtatttgt gggtaaacgc ggcgatctcc tcaaggagag ccttccgatc
     5881 aacccttggc cccggcgccc cagcagaaca gcggtcagcc ccggaattgc tgcgttccat
     5941 gatgatacct ctttgccaga ggaggagtta gcctttccgg aaggggggtg agggtgctct
     6001 cttcggcaga aggcgcggga cgcgttccag gacaaaaacc cgcggctgca gtcgatcgcc
     6061 cctgattgaa gaggggtgcg gagcgtcacg ggcagcagaa ggtttgaatg acatcctcac
     6121 ggccgcatac cggggcaaaa gatgggggta tcaaaagtat gtcccgcccc gctcgaacgc
     6181 ctccctgcgg tcgcgggcca tctgcagcag cgtctcctcg atctccggtc tgccgaggat
     6241 atacccctgc cagagttcgg tgtaggggtg ttcaggcacg acgatgatcc ccttatgctc
     6301 cgcgacccgg tccaggataa gcgatgccgc cctctcgaca ggataagcgt cgtcgggaat
     6361 cctcagttcg ccgtgcgcct ggccgtcgat acccttattg aagataggag tcgctatgtt
     6421 cgccgggcag atcgtcgaga agtagagacc cctatccgca tactcgtacc tcaggcattc
     6481 ggtcagcccc gtgacaccgt actttgtgag ggagtagagc gcctggaacg gaggcggagc
     6541 gatcccggct atcgaactgg tgttcactat gtgcccgaac ccctgtttga gcatgatcgg
     6601 aaccgcggta tggacaccgt atatgacgct ccagaggttg gtatcgatga tagtcttcca
     6661 gtcctcaagg gtggccctct cgaacggcat cgttccaccc acacctgcgt tgttgaagag
     6721 gatatccagt ttcccggctt tttctgccgt cgcctcgata ccccgctgca cctgctcctg
     6781 aacggtcacg tccatgatca ggggctttac ccggtcgccg taggcggtga gctggccgac
     6841 agcctttgca atcttttccg gactgcggcc ggccatgtag acggttgccc ccctctttaa
     6901 gagttcttcg ctgatcgcat accctattcc ggagtttgca ccggtaacga tgcagaccct
     6961 gtctttataa taatcagaat caaccaaaat tctcacccta ttttactcga tgaccctcgc
     7021 gcccatcgca cggaggattg ccctgccctc taccattacc gactgcgtca cccgtttcat
     7081 gggcagggcc gcaagactgt tgatgatgta gtactggaca gggttctgca tcggggtctg
     7141 ctcgatccac tccaggctgc ctcttccggc ctgcgccgtc tccacctcca tgccctgcgg
     7201 gtagaggaca aactggctca gttccgcgcc gggagcgccg acttttggga tatacttcca
     7261 ccccagggtg ttcatggaga cgaactgcga cttcaaagcc tccaggtcag gcccggttat
     7321 cggccctgtg gcctcaaaac cgatccttag aaaggtgttc ccctcgtaac tggcggtcgt
     7381 ggcataatga ggccgcagga tgtgcaggtc atcgatatcc gcatgtatct tcgggatgcc
     7441 ggtctgttcc cgtcccccaa gtatgggtgc ggttctgttc tcccatacca caagcgtgta
     7501 tgcaccgtcg atctcgtcct tctttccgtg aaacctgacc ggcgcagcca cgttgatcag
     7561 gttgtaatgc ccgccctgca accagttgat ctcggtgaac ctgctgaaaa caacctgcac
     7621 ttcaggtgcc aggagttcaa atccttcagg gatatagttt tcaagaaggt ccctcccggt
     7681 ctcgtaactg atcgcaaggg acgccgctct ctgcgtgacc agggtctccg ggtcaaacct
     7741 gctcccgcca aagtggacag gcatcaggta ggtgaagtca tcatgtgttc ggaacatggg
     7801 catccttcca cctgccttac tccctcaggg tatatacctt gtggttcgtt gatcttgaga
     7861 gccatgtctt taaaggccaa tgctgacaga attgaccgac ccgcggccct ggtagtgaga
     7921 gaagaggtgg ttgtggtgtc cgggtcaaaa accgttcgag ccctcgcccg gggacgagag
     7981 ggagcgcagg agataattcc gcaccgggag atccatgcgg ccggaaccgg aaccgaactt
     8041 cgtcgtaaaa gatgcacctc tgcgatcggc atgcagccgg ttctgcctca aaagccatac
     8101 actctgaccg gtcacgttgc taccgcaaga cagataacca tggagatcaa cccactcttc
     8161 acccgatagc ccatacacgg gcgccggtta caggagacga gattggagcg gccgggatgg
     8221 tagaggagac cgccgggaag gagaggcccg gtttaaaaac tcgagcggag tccgacctct
     8281 ctcttgaggt ggtcatactt atagtctttg gcgtattcat gctcctcttc ggcgtgcttc
     8341 tattcaggat tcacacaggt gaacttccgt atgcccccga cagcacctac ggactgtttc
     8401 ttgttatcgt ctcgtttcag ataataacaa tgggaaggac cccattcggc gacctcaggc
     8461 gttcatgggc ggtaatactt ctgggggtct tcacggcggt catcgggatg gtcgcctgtt
     8521 tcataccggg gctgcttgct gaaccggttc gaatccttgt cggtatactg ctctctgccg
     8581 gcggggtctc tctcatgctg cagttgctct tctccaggga taaggccagg ctgtggctga
     8641 aggcccccgg tatactccgg caactcacgc tcgcctgcat cctcgtgtat ctcttctcga
     8701 tcgttcttgg tctggtcacg ctcctccccg gcatcacaac cgatccacag acggcggttc
     8761 tactcataat ctacggcatc agttacttct acctctcatg gtgcatccag aacgccaacc
     8821 gggcgtaccc ccccggagag aggactgcgt gatcaccatg gattccagcg gtccgccttt
     8881 aaaacccggg ctcagattct tcagggagac ctcgatcccc ctctcgattg cgctggtcat
     8941 cccggtcggc gtcctgctgg ttctcctcgg gctgttgctc ttccctgtaa acatgggggc
     9001 gatcccgttc tccccggacg ggcagctcgg gcttctgctg gtcatcatgg ccatccagat
     9061 gcttgcgctg ggggagacgc ccatgggccg ctacaggcgc tcgcacctgc ttgccgccat
     9121 cgggatcgcg ttcgcctcga tggggatctt ctcgtgcata gttcccggcg tcctgaccga
     9181 cgtgatccgg gtgctccttg cgatcctgaa catcgccggc ggagcgatac tccttgcaaa
     9241 acggttcctc ccgatctcgc agggtgccgg caacgctgcg gccgccgttc cggcctctct
     9301 catgccgacc ttcaagagac ttaccgccac ccagacggtg ctgaacgttg tatcgatatc
     9361 gtttggcatc agcatgctgc tgccgggcct catccccggc atggtcattg cggggattgt
     9421 cgtggtcaac ggcctgctcc tatttgcgct ggcggcaatc cttatgaagg tggtttgacc
     9481 gccgcaggcg gcatcaagac cgctccgctg ccggaagaac cgcttgagaa gacgtatgcg
     9541 aggggtgaga tttgaactca cgaactcctg cgagactgga ccctgaatcc agcgcctttg
     9601 acctggcttg gcaaccctcg cgcctgatat cgtgggagtt caaacaaaat aaatgtaatc
     9661 actcctcccg ggcagaggag ttccgggagt tgttctccat gaaccgcttc atctcgagtt
     9721 cacgcagttc gtgccgcctg atcttgccgg agatcgtctt cgggagcgac tccacaaact
     9781 cgaccctgcg gggatacttg tagggcgccg tcacccgttt gacgtagcgc tggatatcct
     9841 tcaccagcgc ctccgagggt ttgtagccgg gtttcaagac gatgaacgcc ttcacgacca
     9901 tcccccggat cacgtcgggc gacccgacaa ccgccgcctc ctggacggcg gggtgctcca
     9961 taagggcgct ctcaacctca aatggcccga tccggtatcc tgacgacttg atgacgtcgt
    10021 catcacgccc gacgaaccag aagtagccgt cctcgtccat ccgtgctttg tcgccggtgt
    10081 agtagaaccc gttctggaac gccctgcggt tctcctcctc gctggcgtcc aggtaacccc
    10141 ggaagagccc caccggccgg gggttgagcc tgatcgctat ccgcccctcc tccatgggac
    10201 cgaccggctt accatcgtcg tcgtggagtt cgatatgcca tccgggcatg ggcctcccca
    10261 tcgaaccggg tttgaccttc atgccgggga gcgtccctat acagagcaca gtctcggtct
    10321 ggccgtagcc ttcgtagatc gtcttgcccg ttccttcctc ccaggccctg ataacctcgg
    10381 ggttgagcgc ctcgccagcg ctgcagcagt ggcgcaactc ggcgagatcg aacttgtcga
    10441 ggtcggccag gatcagcatc ctgtagatgg tcggggggca gcagaacgtc gtcacgccat
    10501 acttctccag gagcggcagg atctcggtgg gattgaactt cccgcggata tcatatacaa
    10561 atatgcaggc accaacgatc cactggccga agagttttcc ccaggcgctc ttcccccagc
    10621 cggtatcgga gagtgtcagg tggaggtcgt tcgggtgcag gtcgtgccac aactgcgccg
    10681 tgacgatgtg cccgaggggg taggagtggt catggagcac catcttcggt tcgccggtgg
    10741 tgccggaggt gaagaagatc aggagagggt cggtcgattt cgtccggtgc ataccgggca
    10801 ggttcaccag tttgtaggag catggggcgg ggtagtcgag ctcgacaggg tagctgaccc
    10861 agccatcccg tttgccgtcg gtcatgagca tcacctcgag cgagtggcac tcgtcacgga
    10921 tctcctcgac cttctctgcg tgctcggcca tggttatgac catcttgatc ccggcagcct
    10981 gtatacggta tttcaggtct tttggtgtca gcatcgtcgt tgcggggcag tagaccgcgc
    11041 cgagttttat gagggctatg acaaatatcc accattctgg gacgcgtggg agcatcagca
    11101 tcacacggtc gcctttcttg atcccgtatt tcaggcagat gttgacggcc tggttcgaga
    11161 ggcgcatcat gtcaaaaaaa gtgtacttct tctcgtttcc tttctggtca acccagatca
    11221 ttgccagttt gttcctgtcc ttctttgccc aggcgtcgac cacgtcaaac ccaaagttgt
    11281 agtattcagg gacatctatc ctgaaattgg cacagagttc ttcgtaggag ggtcggtttt
    11341 gctctacatc agccataacg cgatccctta gaggttgcgc gtttatggtt taaaggattc
    11401 actaaataaa aacaccaagg ccatcaaacc aatac
//
LOCUS       DAOSZX010000043        11279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4915_length_11279_cov_20.728350, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000043 DAOSZX010000000
VERSION     DAOSZX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_11279_cov_20.728350"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="mcrG"
                     /locus_tag="PK196_04995"
     CDS             complement(<1..358)
                     /gene="mcrG"
                     /locus_tag="PK196_04995"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HON40807.1"
                     /translation="MAYKPQYGPGTSIVAENRRKQMNPDYKLERVRSVTDEDVVLILG
                     HRAPGSAYPSAHPPLAEQQEPDCPMRRLVKPTEGAKAGDRVRYIQFTDSMFNAPSQPY
                     QRTYTECYRFRGIDPGT"
     gene            complement(450..917)
                     /gene="mcrD"
                     /locus_tag="PK196_05000"
     CDS             complement(450..917)
                     /gene="mcrD"
                     /locus_tag="PK196_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HON40808.1"
                     /translation="MTDATFPQVRIVPHRLLKPVTAESLLNNLAGITGVRRILVRGPG
                     LPENVPYGPARGNPNPHHDRRTINVGDTEIALRVQVGEVILEVEDDSAITEIRSICDN
                     FFVNYPYQILMGRFMKTDPTLVDYARYGPDADPDLIGLADPRSRDGPVIIRTE"
     gene            complement(934..2238)
                     /gene="mcrB"
                     /locus_tag="PK196_05005"
     CDS             complement(934..2238)
                     /gene="mcrB"
                     /locus_tag="PK196_05005"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HON40809.1"
                     /translation="MAKYTETIDLYSDDGKLLKSGVTLDRISPLVNPATGKIIDLTKR
                     TISVNLGGIQDALRTGKLGKGKIKGRELDLPIMENKDAIVSRIKEMVQVEEGDDTEIL
                     EFNGGKLLLVQVPTKRLINASTYDAAITSVAAATTLAIVDQFNIDAFNASTVKAACWG
                     SYPHTQDMEGALVTSILTIPQNNEGIGYALRNIPVNHVVMMTNKNALQGAALSSTLET
                     AGIFEMGSAIGPFERAQLLAYAYQGLNANNLVYDLVKANGENGTIGTVVQSLVERAIE
                     DKVISPGKKGSYFQFYETKDPMLWNAYAAAGTMAATIVNCGAGRFAQAVSSTLLYFND
                     LLEHETGLPSTDYGRVMGTAVGFSFFSHSIYGGGGPGIFNGNHVVTRHAAGVAIPCVV
                     AAMALDAGTQMFSPESTSKLFAETYGKVDVFNKPIQQIANGA"
     gene            3307..3723
                     /locus_tag="PK196_05010"
     CDS             3307..3723
                     /locus_tag="PK196_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HON40810.1"
                     /translation="MANKITLNMITQRSIEEGVAMEKGKTTPEYFEACSIIELHDEDI
                     KALGLVPNQLVRVTSECGSVVVKVVKGRQTLYPGLAFIPQGVWANQIVPPRTQATGEP
                     QYSGFPVTVEPSARGERRKSALELVQGAVGMWRGEQ"
     gene            3723..5093
                     /locus_tag="PK196_05015"
     CDS             3723..5093
                     /locus_tag="PK196_05015"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HON40811.1"
                     /translation="MPKRIENVGCPYCGVCCDDLVVTVSDDGKKILEVENACAIGNQI
                     YHHATDSERVKLPRLRQPDGTYKEISYDEAIDYAARVLYNARKPLIYGFGSTNCEGMA
                     AAAKVGEKAGAILDNCASICHGSSLLAIFDNGYPSCTLGEVKNRADVILYWGANPAHA
                     HPRHMSRYSIFPRGFFTGKGHKSRKIIVIDPRYTDTARVADIYLQVKQGYDYDLFDAF
                     RTVVRGHEIPEEVAGIKREKIHEVAEIMKNARFGTIFYGMGLCHTDGRNHNIDIAISL
                     TRDLNDFTKWTIMAMRGHYNITGPNQVWSWQYGFPYCLDLSKRDIAHMNPGETSSVDL
                     AMRGEIDAFFNVATDAGAHFPIAAVKELRKHPWITIDPNICMASEISDLHIPVGVVGV
                     EVPGIVYRMDNVPIQYRKVIDPPEGVISDEELFERIYQRLCDLEAEDAAKERAKTTPE
                     GVPAED"
     gene            5097..6803
                     /locus_tag="PK196_05020"
     CDS             5097..6803
                     /locus_tag="PK196_05020"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HON40812.1"
                     /translation="MSEFLIKNGFVFDPLQGIKGDRMEIAVRDGKIVETGALRHPETI
                     DASGMTVMGGGIDIHAHVVGPKVNVGRLFRPEDKLFKSPLKRCSRMEMGFSVPSTFKA
                     GYDYARMGYVFVNEAAMPPLHSPHVHEEIRDTPIIDNAAMPVFANNWFTLEYLKNHEI
                     ENNAAYTAWLLRATRGFAIKAVNPGGTEAWGWGLNCENVHDPVPYFDITPAEIIKGLI
                     ETNEHLGLPHSVHLHCNNLGNPGNYTDTLDSLKLAEGYTPKNNFGREQVLHATHLQFH
                     SYGGDSWKNFESRAPEVIDYVNSHENITIDIGQVTLDETTTMTADGPFEHHLRALNNL
                     KWANTDVELETAAGVVPYIYSPKVKVCTIQWAVGLELALLSKDLMRTFVSTDHPNAGP
                     FTRYPRVITWLMSRAARDATMKTFKWLEKTIDATSIDSIDRELDLYEIAQMTRAGPAK
                     ALGISNTYGGLAPGMDADIAIYPLNPENMPSDPAEIEKAFSRCAWLLKDGVVTVRDGE
                     VLTEPAKRTVWVDVKVPENAQVQRDIYEHFLRYYSVNLDNYSLFEEHLFNPRVIEVDT
                     TE"
     gene            6817..>7598
                     /locus_tag="PK196_05025"
     CDS             6817..>7598
                     /locus_tag="PK196_05025"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HON40813.1"
                     /translation="MKTVTLTMTNPPELYLEGQNITPDAFAGKTAAEIAAIDVWEGKL
                     KSPLGKYFTVEGNGGATAADTKIILRGDCSRLKRVGQQMTAGEIVIEGNADMYIGGWM
                     KGGKIHVKGNVDSFCGLAMEGGELIIDGNAGHHLGSSPRGEWRGMQGGVIRVAGSVGN
                     DTATFMNGGTIIIGGDADIHVSTHAEGGTVIIKGNAKGRVGGQMVKGNIYVFGKIENP
                     LPGFQHIGEVEEEVDGTKARFIHYIGDLGERHPVSRGKPVYG"
     assembly_gap    7599..7698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7699..8697
                     /locus_tag="PK196_05030"
     CDS             <7699..8697
                     /locus_tag="PK196_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HON40814.1"
                     /translation="GFAVKSRETLGASGRNPVRLQNPKRVNTGNAISPGYDAVVMIED
                     VSGDEGGWSTVKAARPGEHIHPAGVEVRQGGLILPAGHWIRPCDIGVLLSCGVTEVEV
                     LEIRVGLIPTGSEVVPAGEIPGPGGVIESNTAVAAAMLGEAGATCTRYGIVADDPGLL
                     REAIERGIRENDLLLVSAGSSAGTRDFTADVIGDLGEVLVHGIAMKPGKPAIIGKIDG
                     KPVIGIPGYPIAAVTAIRELALPLLAAWGLAPRPPQRISARLTETVTADPGYDEFVLL
                     TVSWSGDHYTAAPLPRGSRTQMALVRANAFLHIPAGSGGVIPEGTVVEVEVTGPRV"
     gene            complement(8701..9444)
                     /locus_tag="PK196_05035"
     CDS             complement(8701..9444)
                     /locus_tag="PK196_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40815.1"
                     /translation="MQSAGRHVYMDIEFGYVYGTSREVMMPIEIGAVLHRPEDESLSY
                     AAEQFRYDLDVEIWKKVTDPCGRTAGVETTVSNLSRGECGMPYDHSYRLPDDEVETAE
                     MTAQRAFSDLRDFLESIVSREGATAIIVFAASMERRAFRAAGFSPDGCAFVDLQREIR
                     RRLKMEQFLSLDRLACMIGFSTDGATISSAHFQYPVPQGCRHLLGAHRGLGDAVRTFL
                     VAREFQEHLPYLGERIRNLVETGGGNGEE"
     gene            9646..10353
                     /locus_tag="PK196_05040"
     CDS             9646..10353
                     /locus_tag="PK196_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HON40816.1"
                     /translation="MDSPGRDRAEELRALLARPPFEVTGITDAAIPLYDRALTHRSYS
                     NGGEYPAAVAEDYERLEFLGNYILDFIIAEHLYTGYDLQPGEMNRRLQVTRNTKLAEI
                     VRLQGLGIDRAVRRRGQALTDSIIADAFEALIGAIYLDRGLDAARSVVIAIFEDEIAD
                     CDTDRNYRGRLQEHVSRKNLGYLEYTFRQTGPGSCPTWVARVRVGGILSGEGEARTKQ
                     AAAMIAAREALIRLGKG"
     gene            10356..>11279
                     /locus_tag="PK196_05045"
     CDS             10356..>11279
                     /locus_tag="PK196_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HON40817.1"
                     /translation="MLQPSGRDTRVAVVGCEGYSRSEVDAAVRRAIDLLVVLEGLFPP
                     GEKILLKPDLLHGLEPDRCVTTHPAVVAAIARLLVEHGCRVLIADSPGGGVIYSEANL
                     RRAYARAGYMAAAEETGAALNYDTGSSSVSFPEGAVMRQFSIITPAAEADGIVVVSKA
                     KTHMWTRMTGATKNLFGIIPGLEKPVFHSRFQDERTFGEMLVDLNECMRPRLQVVDAV
                     MGMEGDGPQAGTPRKIGAILAGSHYAAVDTVLARLIGIEPLEIGCIASAAERDLFNPA
                     DVRTVGDDPAALAVPDFRKPYAYAGARGGVGR"
BASE COUNT         2420 a         3215 c         3280 g         2264 t
ORIGIN      
        1 gtgtacccgg gtcgataccg cggaagcggt agcactcggt gtatgtccgc tggtatggct
       61 gtgatggcgc gttgaacatc gagtccgtga actggatgta gcggacgcgg tcaccggcct
      121 ttgcaccctc ggttggcttg acgagcctgc gcattgggca gtctggctcc tgctgttcgg
      181 caagcggcgg gtgtgcagag gggtatgcag aaccaggggc ccggtgaccc agaatcaaca
      241 caacatcttc atctgttact gagcgaaccc tctcaagttt gtagtcgggg ttcatctgtt
      301 tacgtcggtt ctcggcgaca atcgatgtcc cgggaccgta ctggggcttg tatgccattg
      361 tttcattcac ctcaaagttg gtagatacct atatcctctt ttgaatgagt tttcagccat
      421 atttccgaaa tcctgcatct taacagcagt tattcagtcc tgatgataac cgggccgtcc
      481 ctgctcctgg gatcagcgag accgattaga tcgggatcgg catctggtcc atacctcgcg
      541 tagtcaacga gcgtcggatc ggtcttcatg aacctgccca tcaggatctg gtaagggtag
      601 ttcacgaaga agttgtcaca gatcgaccgg atctcggtta ttgcggagtc gtcctcgacc
      661 tcaaggataa cctcaccaac ctgaacgcgc aacgcgatct cggtatcgcc gacgttgatc
      721 gtcctgcggt catggtgagg gttcgggttt cctcgtgccg ggccgtaagg gacgttctct
      781 ggaagacccg ggccgcggac cagaatccgg cggacaccgg taatgccggc aaggttattc
      841 agcaggcttt ctgcggtcac gggtttgagc aacctgtggg gcacaatcct gacctgtgga
      901 aatgtggcgt ctgtcattcg aacctctgtt cgttcaggct ccgtttgcga tctgctggat
      961 cggcttgttg aagacgtcga ccttaccgta ggtctcggcg aagagtttcg aggtgctctc
     1021 gggcgaaaac atctgggttc cggcatcgag cgccatcgcg gcgacgacgc acgggatggc
     1081 cacgcctgcg gcgtgtctgg tcacgacatg gttgccgttg aatataccgg ggcctccgcc
     1141 gccgtagatc gagtggctga agaacgagaa ccccacggca gtacccatca cacggccgta
     1201 gtcggtgctc gggagaccgg tctcatgctc gagcagatcg ttgaagtaga ggagcgtcga
     1261 cgagaccgcc tgcgcgaacc gtccggcgcc acagttgacg atggtggctg ccatggttcc
     1321 tgcagcggcg taggcgttcc agagcatcgg gtccttcgtc tcgtagaact ggaagtaact
     1381 gcccttcttg ccggggctga tgaccttatc ctcgatggcg cgttcgacca ggctctggac
     1441 gacggttccg atcgtcccat tctcaccatt tgcctttact aggtcgtaga ccaggttgtt
     1501 cgcgttcagg ccctggtatg cgtaggcaag caactgggcg cgctcaaacg gcccaatggc
     1561 ggatcccatc tcgaagatcc cggcggtctc gagggtggac gatagcgctg caccctggag
     1621 agcgttcttg tttgtcatca tgacgacatg gttgaccggg atgttgcgga gcgcgtagcc
     1681 gatgccctca ttgttctgcg ggatcgtgag gatggatgtg acaagcgcac cctccatgtc
     1741 ctgggtgtga ggataactgc cccagcaggc cgctttgacg gtggaagcgt taaacgcgtc
     1801 gatgttgaac tgatcaacga tcgcgagcgt tgtcgcggct gcgaccgagg tgatcgcggc
     1861 atcatatgtg gatgcgttga tcaggcgttt tgtcgggacc tggacaagca ggagtttacc
     1921 cccattgaac tccaggatct cggtgtcgtc accctcctca acctggacca tctctttgat
     1981 cctgctgacg atcgcatcct tattctccat gatcgggagg tcaagttctc ttcccttgat
     2041 cttgcccttt ccaagttttc ctgtcctgag cgcatcctgg atgccgccga ggttcacact
     2101 gatcgttctc ttggtcaggt cgatgatctt acctgtggcc gggttaacca gcgggctgat
     2161 tctgtcgagg gtaacgccgc ttttgagcag tttaccatca tcagaatata gatcgattgt
     2221 ttctgtgtat ttagccatag tatttcatcc ttttaccctt gttcaatacg ttctgttcat
     2281 gcaggatacg gcggggtaac gatccgcgcc ggatcctcca gagaccgcta ctgcacgtcc
     2341 cagaagggac tataagggta gagaggatgt gttaataaaa tttgcgaaat aaagtagata
     2401 ttacccttaa aaagtattat ccatgtaaga tttattctta tcatgatatt ttaaaagtag
     2461 gatttttgag accagaataa tctatctgaa atatttaaaa gagtttcggt cgatccggct
     2521 gttatgcggc gcaccaatat gttatgtatg ttggatacgc attaaataat attaaaatat
     2581 atattaataa caatagttca gatagtaatc acaaatagtg cttaaaagta accaatctca
     2641 taagtttgac ccttggaata tataattcta ccctccaagg gctggatttt gaagaccggg
     2701 gtatcctggc cctccgctat tgtatcaaac gtaacgcgtc tctatattat catctgtggc
     2761 tggctgaggg cgtgaacgac ctctcgcctc cagggcgggg cttttggggg ttctcgcaga
     2821 ggaacggcgg atctcgggca ggttcatccg ggaaacgaac gatcctgacc ccctctgccg
     2881 gtgaactgcc tgcaaactat aaagtagggg gcgaggtgga ggcggttttc cgctttcatc
     2941 cccgcacagt ttggtcgggg tcttcccagt tcgccgcctc gccaccgcaa gatcaatcaa
     3001 aaaaccttaa tgtaaatgtt gtaacatata tctacttaca tatgcggaat ggttaaatac
     3061 aaaccattac aagctatgtt aaaacgatcc acccggtgct ccgcccggaa gtttcgtttc
     3121 ccccaaataa gcggggcccc acgtgagggg ggaggacccg cgttccgggc acggcaaatc
     3181 ccggtaaatc ttcctggtat ccagggggcc tgatgaaaag ggcattatcg ggttcaccgt
     3241 gctccacatg gccatctcac cggaactccg ggagaccggc cgcaccaact aaatcgagga
     3301 aaaactatgg cgaacaagat tacgctgaac atgatcaccc agcgttccat cgaggaaggt
     3361 gtcgcgatgg aaaagggcaa aacaaccccg gaatactttg aggcatgctc gatcatcgag
     3421 ctgcatgatg aagacatcaa ggcgctcggc ctggttccta accagcttgt gcgggtgaca
     3481 agcgagtgcg ggagtgtggt tgtcaaggtc gttaagggac gccagactct ctatcctggt
     3541 cttgcattca tcccgcaggg tgtctgggca aaccagatcg tcccaccaag gacgcaggca
     3601 accggggaac cccagtacag cggcttccct gtgacggtcg agccatctgc caggggcgag
     3661 aggcgcaaga gcgcacttga actcgtccag ggggccgttg gaatgtggag aggtgaacag
     3721 taatgccaaa gagaatcgag aacgtcggat gcccatactg cggtgtctgc tgtgacgacc
     3781 ttgtcgtgac cgtatcggac gatggaaaga agatacttga ggtggagaat gcctgtgcca
     3841 ttggcaacca gatctaccac catgcaacag acagcgagag agtcaaattg ccccgtctcc
     3901 gccagccgga cggcacctac aaggagatct cctacgacga ggctatagac tacgcggcga
     3961 gagtactata caacgccagg aagcccctca tttacgggtt cggatcgacc aactgcgagg
     4021 gcatggccgc agcggccaag gtcggcgaga aagcgggtgc catactcgac aactgcgcct
     4081 ccatatgtca cgggagttct cttctggcca tattcgacaa cggttacccg agttgcaccc
     4141 tcggggaggt caagaaccgg gcggacgtca tcctctactg gggtgcaaac ccagcccatg
     4201 cccacccgcg acacatgtcc cgctactcga tcttcccccg tggcttcttc accggcaagg
     4261 gccacaagag ccgcaagatc atcgtcattg acccccgcta caccgacact gccagggtgg
     4321 cggacatcta cctccaggtg aagcagggct acgactacga cctcttcgat gcgttccgca
     4381 cagttgtgcg tggccacgag atccccgagg aagttgccgg gatcaagcgc gagaagatcc
     4441 acgaagtggc cgagatcatg aaaaacgccc gcttcggtac aatcttctac ggcatgggac
     4501 tctgccatac tgatggccgg aaccacaaca ttgacatcgc catcagcctg acgcgtgacc
     4561 tcaacgactt caccaagtgg acgatcatgg ccatgcgcgg tcactacaac atcaccgggc
     4621 caaaccaggt ctggtcgtgg cagtatggct tcccctactg tctggacctc tcaaagcgtg
     4681 acatcgctca catgaatccg ggtgagacca gttccgttga ccttgccatg cgcggcgaga
     4741 tagacgcatt cttcaacgtg gcgaccgacg ccggtgctca cttcccgatc gcggcggtca
     4801 aggaactaag aaagcatccc tggatcacca tcgaccccaa catatgcatg gcaagcgaga
     4861 tctctgacct tcacatcccg gtcggcgttg tcggtgtcga ggtccccggt atcgtctatc
     4921 gtatggataa tgtcccgatc cagtaccgca aggtcatcga cccacccgag ggcgttatca
     4981 gcgacgagga gctcttcgag cggatctacc agaggctctg tgacctggag gcggaagatg
     5041 ccgcgaaaga gcgggctaag accactccgg agggagtccc cgcggaggat tgaggcatga
     5101 gcgaattttt gatcaagaac ggttttgtct ttgaccccct gcaggggatc aagggcgacc
     5161 gcatggagat cgcggtcagg gacggcaaga tcgtggagac aggcgctctg cggcatccgg
     5221 agacgatcga tgcatccggt atgaccgtga tgggaggcgg tattgacatt catgcccacg
     5281 tggtcgggcc gaaggtgaac gtcggccgac tcttccgtcc ggaggataaa ctattcaaga
     5341 gtcctctgaa acgctgcagc cgcatggaga tgggtttctc cgttccctcc accttcaagg
     5401 ccggttacga ctacgcacgt atgggctatg tctttgtcaa cgaggcggcg atgccgccgc
     5461 tccactcacc ccatgtccac gaggagatcc gggatacccc gatcatcgac aacgccgcga
     5521 tgccggtctt cgctaacaac tggttcaccc ttgaatacct caagaaccat gagatcgaga
     5581 acaacgcagc gtatactgcc tggctactcc gtgcaacccg ggggttcgcc atcaaggccg
     5641 tcaatcccgg tggaaccgag gcctggggat gggggctgaa ctgtgagaac gtccacgacc
     5701 ctgttccgta cttcgatatc accccggcag agataatcaa gggtctcatc gagacaaacg
     5761 agcatctcgg tctcccacac tctgtgcacc tgcactgcaa caaccttggc aaccccggca
     5821 actataccga cacgctcgac tcactcaaac tcgccgaggg atacaccccg aagaacaact
     5881 tcggccgtga acaggtgctc cacgccactc acctccagtt ccactcctat ggcggtgatt
     5941 catggaagaa cttcgagtcg cgtgcgcctg aggtcatcga ctacgtgaac tcccacgaga
     6001 acatcaccat cgacatcggc caggtgactc tcgatgagac caccaccatg accgcggacg
     6061 gcccgtttga gcaccacctg cgcgccttaa acaacctgaa gtgggcaaac accgacgttg
     6121 aacttgagac cgctgcagga gtcgtgccct acatatacag cccgaaggtt aaggtctgca
     6181 ccatccagtg ggcggtgggc cttgagcttg cccttctctc gaaggacctg atgcggacgt
     6241 ttgtctccac cgatcacccg aacgccgggc cgtttacccg ctacccacgg gtcatcacct
     6301 ggctgatgag cagggctgca cgcgatgcga ccatgaagac gttcaagtgg cttgagaaga
     6361 cgatcgatgc aacctctatc gacagcatcg accgcgaact cgatctctac gagatcgcgc
     6421 agatgacccg tgcagggccg gcaaaggcgc tcggtatcag caacacatac ggcgggcttg
     6481 cacccggcat ggacgctgat atcgctatct accccctcaa cccggagaac atgccctctg
     6541 atccggcgga gatcgagaag gccttctccc gctgcgcctg gcttctgaaa gacggcgttg
     6601 tgacggtcag ggatggcgaa gtcctcacgg agcctgcaaa gcggacagtc tgggtcgatg
     6661 tcaaggttcc ggagaacgcg caggtgcaga gagacatcta cgagcacttc ctccgctact
     6721 acagcgtgaa ccttgacaac tactcgctct tcgaggagca cctcttcaac ccacgggtga
     6781 tcgaggtcga tacgacagaa tgaggtgatg agaagcatga agacagtaac cctcaccatg
     6841 accaaccccc ccgagctcta ccttgagggg caaaatatca ccccggacgc ctttgcaggc
     6901 aagactgcag ccgagatagc tgccatagac gtctgggagg gcaagttgaa gagcccgctc
     6961 ggcaagtact tcaccgttga gggcaacggt ggtgcaacgg cggctgatac gaagatcata
     7021 ctccgtggag actgctcccg tctcaagaga gtcggtcagc agatgactgc cggtgagata
     7081 gtcatcgaag gcaacgctga catgtacatc ggtggctgga tgaaaggcgg gaagatccat
     7141 gtcaaaggaa acgtcgactc tttctgcggc cttgccatgg aaggcggcga acttatcatc
     7201 gacgggaacg caggacacca cctcggatca tctccccgcg gtgagtggcg cggcatgcag
     7261 ggcggagtta tccgggttgc cggcagcgtc gggaacgata cggccacgtt catgaacggc
     7321 ggaacgatca tcatcggcgg cgatgccgac atccacgtat ccacccacgc cgaaggcggc
     7381 accgtcatca tcaaggggaa cgccaaaggc cgggtcggcg ggcagatggt gaaaggcaat
     7441 atctacgtct ttggaaagat cgagaatccg ctcccgggat tccagcatat cggggaggtc
     7501 gaagaggagg tcgacggtac gaaggcccgc tttatacact acatcgggga tctcggcgag
     7561 cgccaccccg tctccagagg caagcctgtg tatgggaann nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnngg ttttgccgtc aaaagccgtg agacgctcgg tgcaagcggg
     7741 agaaacccgg tgagacttca gaatcccaaa agggtgaata ccggaaacgc aatctctccc
     7801 ggctacgacg cggtcgtcat gatcgaggat gtcagcggtg acgagggggg atggagcacc
     7861 gtgaaggcgg cgcgccccgg cgaacatatc catcctgcgg gggtcgaggt ccggcagggc
     7921 ggcctcatcc tcccggcagg gcactggata cgtccctgcg acatcggcgt cctcctatcc
     7981 tgcggcgtta ccgaagtgga ggtgctggag atcagggtcg ggctcatccc gacggggagt
     8041 gaggtggttc ctgcgggtga gattccgggt cccggtggcg tcatcgagag caacaccgcc
     8101 gttgcagcgg cgatgctagg tgaggccggg gcaacatgca cccgttacgg gatcgttgct
     8161 gacgatcccg ggcttctgcg ggaggccatt gaacgcggga tacgtgaaaa cgacctgctt
     8221 ctggtctccg cagggtcatc ggcaggcacc cgcgacttca ccgcagatgt aatcggagac
     8281 cttggggagg tgctcgttca cgggatagca atgaagcctg gaaaaccggc gatcatcggc
     8341 aaaatcgatg gtaaacctgt tattgggatc ccgggctacc cgatcgccgc cgttacggcc
     8401 atacgggaac ttgccctccc cctcctcgcg gcgtggggtc tggccccccg gcccccgcag
     8461 cgtatcagcg cccgccttac agagacggtc acggcggacc cgggctacga tgagtttgtt
     8521 ctcctcaccg tctcctggag cggcgatcac tacaccgctg cacccctgcc ccgcggctcc
     8581 agaacacaga tggccctggt gcgtgcaaac gcctttctgc atatacccgc gggcagcggc
     8641 ggggtaatac ccgaaggcac ggtggtcgag gtggaggtga cagggccgcg cgtatagatt
     8701 tcactcctca ccattcccgc cgccggtctc caccaggttt ctgatacgct ccccaagata
     8761 gggcaggtgc tcctggaact cgcgtgcaac caggaaagtc cggacggcgt cccccagtcc
     8821 acggtgggcg ccgaggaggt ggcggcagcc ctgcgggact gggtactgga aatgggccga
     8881 ggagatggtg gcgccgtcgg tggagaaccc gatcatgcag gccagacggt ccagggatag
     8941 aaactgctcc atcttcaaac gtcgacggat ctcccgctgc aggtcgacaa acgcacagcc
     9001 gtcaggggaa aacccggccg cgcggaaggc ccgcctctcc atactcgctg caaagacgat
     9061 gattgctgtg gccccttccc ttgaaacgat cgattccagg aagtctcgca ggtcgctgaa
     9121 cgccctctgt gccgtcatct ctgcagtctc aacctcgtcg tcggggagac ggtaggagtg
     9181 gtcgtatggc attccgcact caccccgact caggtttgag acggtcgtct cgacaccggc
     9241 cgtcctgccg caggggtcgg tcaccttctt ccagatctcg acatcgagat cgtagcggaa
     9301 ctgctcagcc gcgtaagaaa gactctcgtc ctcaggtcgg tgcaggacag caccgatctc
     9361 gatcggcatc atcacctccc tggaggtgcc atatacgtaa ccgaactcta tgtccatgta
     9421 gacatgcctg cctgccgact gcatcaggtg caggatctct cccgcgggag agaaaaggtt
     9481 tcgcggccgg ccggtctcca gcaaaggttt atcctggtga acgaaagcca ttctatagaa
     9541 gaagagcggg attgcgcccc ccccagatag attaccatga aactctccat caactggccc
     9601 ggatctcgta tcagtagact tttctggcga acgcgccgcc tccgtatgga ctctccaggc
     9661 cgcgatcgtg cagaggaact acgtgcgctt ctcgcccgcc ctccgttcga ggtaaccgga
     9721 atcaccgatg ctgctatccc tctctatgac cgtgcgctga cccaccggtc gtattcgaac
     9781 ggcggtgagt atccggcagc ggtggctgag gactacgaga ggcttgagtt tctcgggaac
     9841 tacatccttg attttatcat cgccgagcac ctctacaccg gctacgacct ccagccaggc
     9901 gagatgaacc ggaggctcca ggtgacacgg aacacgaaac ttgccgagat cgtgcggctg
     9961 caggggctcg gtatagaccg tgctgtcagg cggcgggggc aggcgttgac cgactcgatc
    10021 atcgccgatg cttttgaggc tctgatcggt gcgatctacc ttgaccgtgg cctcgatgcg
    10081 gccaggagcg tggtgatcgc gatctttgaa gatgagatag ccgattgcga cacagaccgg
    10141 aactaccgag ggaggctcca ggaacacgtc tcaaggaaga accttggtta cctagagtat
    10201 accttccgcc agaccggtcc cgggagctgc ccgacctggg ttgcccgggt gagagtcggc
    10261 gggatcctct ccggagaagg ggaggcccgg acgaagcagg ctgcggccat gatcgcggca
    10321 agggaggcgc taatacgcct cgggaagggc tgaaagtgct gcaaccgtcc gggagggaca
    10381 cacgagtcgc tgttgtcggg tgcgagggtt acagccggtc tgaggtcgat gctgccgtca
    10441 ggcgcgcaat cgaccttttg gtggtattgg aaggtttgtt tccccccggg gagaagatcc
    10501 tccttaagcc agacctttta catgggcttg aaccggatcg gtgcgtcaca acccacccgg
    10561 cggtcgtcgc cgctatcgct cgcctcctcg tggagcacgg ttgccgtgtt ctcatcgcag
    10621 acagcccggg aggcggggta atctacagcg aagcgaacct tcgacgggct tacgcccgcg
    10681 ccgggtatat ggcggcggcg gaagagaccg gggccgccct taactatgat accggttcaa
    10741 gtagcgtctc ctttcctgag ggagcggtga tgagacagtt ctccatcatc accccggccg
    10801 cggaggctga cgggatcgtg gtcgtctcaa aggccaaaac ccatatgtgg accaggatga
    10861 ccggcgccac aaaaaacctc tttggcataa tcccgggtct cgaaaaaccg gtctttcact
    10921 ccaggttcca ggacgagcgg acattcgggg agatgcttgt cgacctcaac gagtgcatga
    10981 ggccacgact tcaggttgtg gatgccgtca tggggatgga aggagacggt ccccaggcgg
    11041 gaaccccccg aaagatcggg gcaatcctgg ccgggagcca ctatgcggcc gtggacaccg
    11101 ttcttgcgcg gcttataggg atcgagcccc ttgagatcgg ctgcatagca agcgcagccg
    11161 aacgcgacct cttcaaccct gcagacgtca ggacggtcgg cgatgaccct gccgccctcg
    11221 ctgttcccga cttccggaaa ccctatgcat atgctggcgc ccggggtggc gtcgggcgg
//
LOCUS       DAOSZX010000044        11162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_4982_length_11162_cov_23.032952, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000044 DAOSZX010000000
VERSION     DAOSZX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_11162_cov_23.032952"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..81)
                     /locus_tag="PK196_05050"
     tRNA            complement(7..81)
                     /locus_tag="PK196_05050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Ile,seq:cat)
     gene            183..1022
                     /locus_tag="PK196_05055"
     CDS             183..1022
                     /locus_tag="PK196_05055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON40818.1"
                     /translation="MHRIINWNELWKAIYINSPERLDKSRDPAAAWDRRAAAYRRVTR
                     GEKEATRLELEILNLQGGETVLDVGAGTGRLAVPIAMTAAHVTALDPSEGMLNVLRER
                     MAAEGLTNYSCVKMRWEDAVIGRDIEPHDLVIAAFSLGFYDLAAALEKLDSAATRAVH
                     LFWHAGEWRGPEEMALYRAVFGEEATMRKGYPDYIFPVNILHDAGIYPNVRIYNAVWE
                     TVYDSPEEAARTWVAMHNPRMEDLSPVRDYFARVLYRDEAGKYVETTRRPTAAVWWEK
                     EGR"
     gene            complement(1035..2036)
                     /locus_tag="PK196_05060"
     CDS             complement(1035..2036)
                     /locus_tag="PK196_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HON40819.1"
                     /translation="MEFRKVQRTGGSSYVISLPKEWVQSMNIKKNEPLPVKVQADGSL
                     LILPRPSEDKKSWEKEFQVDSIKDPVFLFRCLVGSYIAGYTAIRISASTRLSPAIRKA
                     VRDFSSMTIGQQIVEETDSFILIRDILNPAEMPFENSIRRVFVIIGTMYNDAMTALEA
                     RDPGLAEDIINRDVEVDKLQWLVSRQYHLMFRDATLPEKLHVTVEGASSYYTVSRSLE
                     RIGDHGVRIASHVPVLLKHKVGDEVVHAMKAATETAMAILDSSMRSLFSSDIVDANRT
                     IDMVGDLTRMTDGISALAHEKTGEVALSIGYIAESIRRAGEYSADISEAIINQIIRD"
     gene            2170..3033
                     /locus_tag="PK196_05065"
     CDS             2170..3033
                     /locus_tag="PK196_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HON40820.1"
                     /translation="MTGKHLSGVTAVALAGTLMVVLMAAGCLGSQPSGSPASPPQQIT
                     ATGSTTVLPIAEKAREAFEDLYPSTSVLVSGGGSSVGVRAVGEGTADIGMSSRDLKPE
                     ETGMYPGLVTHHIADDAVILIVHPENSIESLTLDQVRGIYNGTYTNWNQVGGPDLDIV
                     VVSRDSASGTREFFAEYVMNKEDFTKNQEEFNSNGGVQQKVSQTPGAIGYVGLGYTDG
                     VKPLKVVVNGDPIEPTLENITSKRYPITRPLYMLTNGEPGGAVKEFLDFIMSEEGQQI
                     VASADYIPVQG"
     gene            3068..4969
                     /gene="pstC"
                     /locus_tag="PK196_05070"
     CDS             3068..4969
                     /gene="pstC"
                     /locus_tag="PK196_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HON40821.1"
                     /translation="MKREEATAVQSGYPGWPGHSWRGPEAPFARRNRHLTDLIVGKLL
                     FIVAVLAILVVFFIIGFLLRDGYQIVLETGIWNFLGGVDWNPAGSPPSFGILPLIVGT
                     ILVTALAMAIAVPLSMATAIYIAEIAGPGTKAVVKPAVELLAGIPSVVYGFFGLILLT
                     DWIRILFDRPSGSSWLAGSILLAIMAIPTITSVAEDALSSVPREFREGSLALGATRWQ
                     TIRSVIVPGAVSGISAAVILGMGRAIGETMAVLMVTGNAAVIPDPITNVFSPVRTLTG
                     TLGIEMGEVAFGSLHYHALFGVAVVLLAITLAVNGLSRLIIRRMQRTPARSRAPGPLM
                     QAVHGLIPVSLPRLARRRIIHPKTTQKFAFLLISIGVVIVLAALGVILLEIIVNGAGA
                     ISLEFLTAPPSDLGRAGGIFPAIVGTLYLVAGALAVALPLGIATAIYLIEYTADTRLT
                     RGIRAAVDLLNGTPSIVFGLFGFAFLVLFLNFGVSLIAGQITLGLMVLPTIIRTTEES
                     LRAIPKSLREGSYALGASKWQTVWRVVLPPALPGIITGAILSIGRAAGETAPIMFTAA
                     VFSSRYLPSSLFEPVMSLPYHLFILTTSVAGADDQKFGTALVLLMMVLSIYLIAILVR
                     HRYNRATVV"
     gene            4973..5734
                     /gene="pstB"
                     /locus_tag="PK196_05075"
     CDS             4973..5734
                     /gene="pstB"
                     /locus_tag="PK196_05075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HON40822.1"
                     /translation="MDDSTILETRDLNLWYGPKQALLDISIRIPKNTVTALIGPSGCG
                     KSTLLRCFNRMNDLIDSARITGRILFEGDDIYADHTDVYTIREKIGMVFQKPNPFPKS
                     IYENVAYGPRIHGVRRKEVLDDVVETSLKRAALWEEVEDRLHEPALALSGGQQQRLCI
                     ARSLAVEPRVILMDEPCSALDPIATAKIEDLIIRLARDYTVVIVTHNMQQAARVSEYT
                     GFMYMGRLVEFDRTQRIFEDPTEELTENYITGRFG"
     gene            5743..6396
                     /gene="phoU"
                     /locus_tag="PK196_05080"
     CDS             5743..6396
                     /gene="phoU"
                     /locus_tag="PK196_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HON40823.1"
                     /translation="MVEKFRAELRSLKGEVVEMGDLALDMLQRAVKALETRDVGAVKS
                     FDRDKKRLADQDHEIEQECLRLIALYQPVAKDLRTIATALKMNTYLYRIGRYGKDIAI
                     LVPDLADAPVSSDCLDGIPVMADLVIGMVTDALTAFEREDLSVIDDILAREEEVDALR
                     YDVLRESLSRMVDDPGRITWCTDCIMIARYLERCGDHACKIAEKVHYMVTGERVEIR"
     gene            6777..8246
                     /locus_tag="PK196_05085"
     CDS             6777..8246
                     /locus_tag="PK196_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HON40824.1"
                     /translation="MQDQQPVNLKGIAWTAMQQYGFVPAFPPSVLREVERLKPKVFPA
                     IIDDPRDLRTLPWSSIDNYDSKDLDQIEVCEEGPGGEIRIRVAIADVDAYVPKGSETD
                     RHAARNGTAVYTGVTTFPMLPDRLSAGLTSLLPGQERLAVVIEYTVLPDGSVMPGDVY
                     RAVVANQAKLVYEEVGDWLEGSGPVPDMIRERPDLMRQILLQDQAATRMKSYRTERGA
                     LVLETIEPEPLVEDDRVVALVIQRQNRARCLIEEFMVAANRTLTGFLNGAGLPMIHRV
                     VRKPKYWEEIVAVAAERGTALPEEPDAEALTRFLIRQKAADPERFPDLSLTVIKLMGA
                     GEYVAFRPGEEPVGHFALAVTDYTHGTAPNRRYVDIIIQRLVKSVLAGEPEPPYAPEE
                     LDELASWLTGRDKAAQKVERFVRKAAAAVLLRDRIGETFEAFVTGASDKGTYVRLIDP
                     PAEGRVVIGERGLRVGQRIRVRLLAVDPYKGYIDFGYTR"
     gene            complement(8264..9085)
                     /locus_tag="PK196_05090"
     CDS             complement(8264..9085)
                     /locus_tag="PK196_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HON40825.1"
                     /translation="MALGDNFSLSTPASLSSITPESVVLALIVAVIGLIVVRVIRSAF
                     KTMLSRASKLSGLVVEFLVRFFSILLYVILILVVMATLGFDVSSVVLGLSAVIGLILG
                     LGLQDTITNLAAGVWVAAFRPIDKGEFAEVNGISGTVMGVGIMMTEMLKPDNTYITIP
                     NSQVWGNPVINYSRMETRRLDVTVGVAYDTDLNKAIRVATDLMKSHEGVLPSPEPVTV
                     VTELADSSVNLSLRAWTKTEDLWGVKWDLTREIVGAFRDAGIEIPFPQVDVHLDR"
     gene            complement(9213..10718)
                     /locus_tag="PK196_05095"
     CDS             complement(9213..10718)
                     /locus_tag="PK196_05095"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HON40826.1"
                     /translation="MRDAEILMNPNRLVRFLKKDPAEFTKEDIICFCEANGVEMVNFR
                     YAAEDGKLKTLNFVITSRDHLDTILSDGERVDGSNLFSFIEAGSSDLYVIPRYRTAFV
                     SPFSEVPTLEVLCSFYDVDGKPLESSPGYVLRKANEEFTRRTGYTFMTMGELEYYVIS
                     PRDDLYPATDQKGYHSSGPFVKFADLRDEAMRLIACAGGRVKYGHSEVGCFYDGDTYY
                     EQHEIEFLPMPVEQAVEQLIIAKWVLRMLGDRYGVEISFAPKITVGKAGSGMHFHMLV
                     EKDGRNLLVEEGRLSPLARKMIAGILDVSDALTAFGNTIPLSYLRLVPHQEAPTTVCW
                     GDRNRSVVIRVPLGWIGADGMTRDANPAEKGHVRDRPSKQTFEYRVADGSADLYLTVA
                     GLIVASLHGIEMPDALEAAERLYVSGNIFSPAFKERLAEFRQLPASCVESAGALEAKR
                     AIFEKYGIFPPGMIDSRIAALKAFDDLGLSERLYGNKEAIRELVNKFLHVA"
BASE COUNT         2433 a         3277 c         3262 g         2190 t
ORIGIN      
        1 aaataatggg cccggaggga tttgaaccca cgaccgctcg gttatgagcc gagcgctcta
       61 accggactaa gctacgggcc ctacaactcc cttataaaca tcgatctgtc acgataaata
      121 cgttgtgcgc accacgccga agatgcggac agtatataca atcgcagaac gagcaaaaca
      181 gtatgcaccg gatcatcaat tggaacgaac tctggaaagc aatctacata aactcacccg
      241 aacggctcga taagagccgt gatcccgctg ccgcctggga cagacgtgcc gctgcctaca
      301 gacgcgtcac ccgcggtgag aaagaagcaa ccaggctgga acttgagatc cttaacctcc
      361 agggcgggga gaccgtgctc gacgttggtg caggcacagg tagacttgcg gtgccgattg
      421 cgatgaccgc agcccacgta acggccctcg acccatccga aggcatgctc aacgtcctcc
      481 gggagcggat ggcagcagaa ggactcacga actactcctg cgttaagatg cgctgggagg
      541 atgccgtgat agggagagat atcgagcccc acgaccttgt catcgctgca ttctcgcttg
      601 ggttctacga ccttgccgcc gccctggaaa aactcgactc cgctgcaacc cgggcggtcc
      661 acctcttctg gcatgcgggg gagtggcgcg gcccggagga gatggcgctc tatcgtgcag
      721 tcttcggaga agaagctacc atgagaaagg gctaccccga ctacatcttc ccggtaaaca
      781 ttctccatga tgccggtata tacccaaacg tcagaatcta caacgccgtc tgggagaccg
      841 tctacgactc tccagaagag gccgcccgga catgggtggc gatgcacaac ccccgtatgg
      901 aggatctctc tccggtcagg gactactttg ccagggtgct ttacagagac gaggccggga
      961 agtatgtcga gacgacgcga cgccccacag cggcggtctg gtgggagaaa gagggcagat
     1021 agagggtgac ccattcaatc ccggatgatc tggttgatga tcgcttcaga gatgtcggcg
     1081 gagtactccc ctgcccgccg gatgctctca gcgatgtagc cgatcgagag ggcaacctcg
     1141 ccggtttttt cgtgcgcaag agcgcttata ccatcagtca tcctggtaag atccccaacc
     1201 atgtcgatgg tgcggttggc atcgactata tcggatgaga agagagaccg catacttgaa
     1261 tccagtatcg ccattgcggt ctccgtggcc gccttcatag cgtggacaac ctcatcaccg
     1321 accttgtgct tgagcaggac gggaacatgg gatgcaattc ggacaccgtg gtcgccgatc
     1381 cgctccaggc ttcgactgac ggtataataa cttgatgcgc cttcaacggt cacatgtagt
     1441 ttttcgggga gtgtcgcgtc cctgaacatg aggtgatact ggcgcgagac cagccactgg
     1501 agtttgtcca cctcaacatc ccggttgatg atatcctctg ccagccccgg gtccctcgcc
     1561 tcgagtgcgg tcatggcgtc gttatacatc gtcccgatta taacgaacac ccgtctgatg
     1621 ctgttctcaa acggcatctc cgccggattc aggatgtccc ggatgaggat gaatgaatca
     1681 gtctcctcga cgatctgctg gccgatggtc atcgaactga agtcacggac ggccttgcgg
     1741 atggcggggg agagcctggt ggacgcggat atgcggatgg ccgtataccc ggcgatgtag
     1801 gagcccacaa ggcaccggaa gagaaagacg gggtccttga tggagtcgac ctgaaactcc
     1861 ttctcccacg attttttatc ctccgatggg cgggggagga tgagaaggct tccgtcggcc
     1921 tggaccttaa caggaagagg ttcgtttttc ttgatgttca tagattgaac ccactctttg
     1981 gggagggaga taacgtatga agacccgccc gtcctctgga ccttccgaaa ctccataaga
     2041 gaggtgtttc tatagaagta gaaataatta actgttgtca caatattgaa tataccagta
     2101 tttaccgtat agacgttacc atatttaatg tcgaaaatca ctatactgtt ttaccatcca
     2161 cgtattatca tgacaggaaa acatctgtcg ggtgtgacgg cggttgctct cgccggcaca
     2221 cttatggtag ttctgatggc agccgggtgt ctcggcagcc agccgtccgg gtcaccggca
     2281 tcaccgccgc agcagatcac ggcaaccgga tcgacaacgg tgcttcccat cgccgagaaa
     2341 gcaagggaag ccttcgaaga cctctacccc tccaccagtg tcctggtaag cggcggcggt
     2401 tcaagcgtcg gtgtcagggc cgtgggtgaa gggaccgcgg atatagggat gtcctcgcgt
     2461 gacctgaaac ccgaagagac cgggatgtat ccgggactcg tgacgcacca catcgccgac
     2521 gatgcagtaa ttctcatcgt ccacccagaa aacagcatcg agagtctaac cctggatcag
     2581 gtgcgcggca tatacaacgg cacctacacg aactggaacc aggtgggcgg gccagacctg
     2641 gatatagtcg ttgtcagcag ggacagcgca tcaggaaccc gtgagttctt tgctgagtac
     2701 gtgatgaata aagaagactt caccaaaaat caggaggagt tcaactcaaa cggcggtgtc
     2761 cagcagaaag tctcccaaac cccgggcgcg atcggctacg tgggtcttgg ctacaccgac
     2821 ggcgtgaaac cgctgaaggt tgtggtcaac ggagatccca tcgagcccac tcttgagaac
     2881 atcaccagca agaggtatcc gatcacccga ccgctctaca tgctgacgaa cggcgagccc
     2941 gggggtgctg taaaagagtt cctggacttc atcatgagcg aggaagggca gcagatcgtt
     3001 gcgagcgccg attacatacc agtgcagggg taacctcccc ctcttaccgc cctgaccgtg
     3061 accaactatg aagagagaag aagcaacggc agttcagtcc ggctatcctg ggtggcccgg
     3121 acactcctgg agagggcctg aggctccatt tgccaggaga aaccgccacc tcaccgatct
     3181 catcgtcggt aaactcctct tcatcgtcgc ggtgctcgcg atcctcgtcg tcttcttcat
     3241 catcggtttc ctgcttcgcg acggctacca gatagttctc gagaccggta tctggaactt
     3301 cctgggcggc gtggactgga accccgccgg gtcgccgcca tcgttcggga tactcccgct
     3361 catcgtcggc accattctgg tgacggcgct cgcgatggcg atagccgtcc cgctcagcat
     3421 ggccactgcc atctacatcg ccgagatcgc cggtcccgga actaaagcgg tcgtaaaacc
     3481 cgctgtcgag ttgctggcag gcatcccctc ggttgtctac gggttcttcg ggctgatact
     3541 cctgactgac tggatccgga tcctctttga ccgcccgtcc gggtcgagct ggcttgcagg
     3601 ctcgatcctg cttgcgatca tggccattcc gaccatcaca agcgtcgcgg aggacgccct
     3661 cagttctgtc ccccgtgagt tcagggaggg cagccttgca ctcggggcaa cccggtggca
     3721 gaccatcagg agcgttatcg tgcccggcgc cgtctccggg atcagtgcgg cggtcatact
     3781 ggggatgggg cgtgccatcg gcgaaactat ggcggtactc atggtcaccg ggaacgccgc
     3841 cgtcatccct gacccgataa ccaacgtctt ctccccggtg cggacactca ctgggacgct
     3901 cgggatagag atgggcgaag tggcgttcgg aagcctgcac taccacgcgc tcttcggcgt
     3961 cgcggttgtc ctgctggcga tcacacttgc cgtcaacggc ctgtcaaggc tcatcatacg
     4021 ccggatgcag agaacaccgg cacgttccag ggcgccgggg ccgttgatgc aggccgtcca
     4081 cggcctcatc ccggtttccc tcccccgcct ggccagaagg aggattattc acccgaaaac
     4141 cacacagaag tttgcgtttc tgctgatctc aatcggggtc gtgatcgtgc ttgcggctct
     4201 cggggtgatc ctgctcgaga tcatcgtcaa cggggccggt gcgatcagtc tggagttcct
     4261 gaccgccccg ccgagcgacc tcgggagagc gggagggata ttccctgcga tcgtcgggac
     4321 gctctacctt gttgcagggg ctctggcagt agcgctcccc ctcggcatag caaccgccat
     4381 atacctgatc gagtacacgg ccgatacccg gctgacgcgg gggatccgtg ctgcggtcga
     4441 tctgctgaac gggacaccat ccatcgtgtt cgggctgttc gggtttgcgt ttctggtcct
     4501 cttcctgaac tttggggtct cgcttatcgc cgggcagata actctgggcc tgatggtcct
     4561 ccccaccata ataaggacga ccgaggagtc gctcagggcg atcccaaaat cccttcggga
     4621 agggagttac gcgctcgggg cctcgaagtg gcagactgtc tggcgggtgg tgctcccccc
     4681 tgccctcccc gggatcatca cgggggcgat cctctcgata ggccgcgccg ccggggagac
     4741 cgcgcccatc atgttcaccg cggcggtctt cagcagcagg tatcttccgt cctcgctctt
     4801 cgaaccggtg atgtcgctcc cctaccacct attcatcctg acgacgagtg tagcgggggc
     4861 ggacgaccag aagttcggga cggcgcttgt gctcctgatg atggtgctct cgatatacct
     4921 catcgcaata ctcgttcgcc acagatacaa ccgggcaacg gtggtataga caatggatga
     4981 ctcaacgatt cttgagacaa gagacctcaa cctctggtat ggcccgaaac aggccctcct
     5041 cgatatctcg atacgcatcc ctaaaaacac ggtcacggcg ctgatcggtc cttcggggtg
     5101 cggtaaatcg acacttctcc ggtgtttcaa ccggatgaac gacctgatcg actcggcacg
     5161 aattaccggc cggatcctct ttgaggggga tgacatctac gccgatcaca cggatgtata
     5221 cacgatccgc gagaagatcg gtatggtgtt ccagaaacca aatcccttcc caaagagcat
     5281 ctacgaaaac gttgcctacg gccccaggat ccatggggtg cggcgcaaag aggtgctcga
     5341 cgatgtcgtc gagaccagcc tgaaacgcgc cgcgctctgg gaggaggtgg aagaccggct
     5401 ccacgaaccc gcgctcgccc tctccggcgg gcagcaacaa aggctctgca tcgcccgatc
     5461 gctcgcggtc gaaccgcggg tcatcctgat ggatgaaccc tgttccgccc tcgaccccat
     5521 cgccacggca aagatcgagg accttatcat caggctcgcc cgggactaca ccgtcgtcat
     5581 agtcactcac aacatgcagc aggcggcgcg ggtcagcgag tatacggggt tcatgtatat
     5641 gggcaggctg gtcgaattcg ataggacgca gcggatcttt gaggacccga cggaagaact
     5701 gacggagaac tacatcaccg ggcggttcgg gtaggatgat tcatggttga gaaatttcgt
     5761 gccgaactga ggtccctgaa aggggaagtc gtggagatgg gggatctcgc acttgatatg
     5821 ctgcagcggg cggtaaaagc gctggagacc agggatgtgg gtgcagtcaa atcgtttgac
     5881 cgcgacaaga agagactcgc ggatcaggat cacgagatcg agcaggagtg cctcaggctc
     5941 atcgcgctgt atcagccggt ggcaaaagac ctgcggacga tcgcaacagc gcttaagatg
     6001 aacacctacc tctaccgtat cgggcgatac ggaaaagata tcgctatcct ggtgcccgat
     6061 ctcgccgatg ccccggtgtc atcagactgc ctcgacggca ttccagtgat ggcggatctc
     6121 gtcatcggga tggtcaccga tgcgctgacc gcgttcgaac gggaagacct ctccgtgata
     6181 gacgatatcc tggcgaggga ggaggaggtc gatgccctca ggtatgacgt cctccgggag
     6241 tctctctccc gtatggtgga tgaccctgga aggatcacat ggtgcacgga ctgtatcatg
     6301 atcgcccgct acctcgaacg ctgtggcgac cacgcctgca agatcgccga gaaggttcat
     6361 tacatggtga ccggcgagcg ggtcgagatc cggtgaacct ctcccgccga ccaccccgcc
     6421 atcaggggtg ggcggacagt aggtggcgat agccggtggg gagccgccgt ggccgggacg
     6481 cagatgcgat ctctgccgac cctaccgcga accgaagtat aaaccgtttc ctggcacttt
     6541 atcgggagaa tatcaccatt tgggaggggc tgacggggag gggtcacgaa aagaaatctc
     6601 ttgcaaaacc tgggtcccat cgagatccgg atgcgggcga gcggcccgcg gcgattgcgc
     6661 acatcccctc cggggcggtg cctgaaggaa ctcgtttgat agcctgccat aatcaagtgt
     6721 ggcctgcgag agtttcacag gccattattt ataccctgag acaaatttct caagagatgc
     6781 aggaccaaca gcccgtcaac ctcaagggca tcgcctggac ggcgatgcag cagtacggtt
     6841 tcgtcccggc cttcccccca tccgtcctcc gcgaggtcga aaggctcaaa ccgaaggtct
     6901 tcccggcgat catcgacgac cctcgtgacc tccgaacgct cccctggtca tcgatcgaca
     6961 attacgactc caaagacctg gaccagatcg aggtctgtga agaggggcct ggcggagaga
     7021 tccgtatccg ggtcgcgatc gcggatgtag acgcctacgt cccgaaggga tctgagactg
     7081 atcgccatgc cgcccggaac gggacggcgg tctacaccgg ggttacaacc ttcccgatgc
     7141 tgcccgaccg cctctctgcc ggcctgacat cgctccttcc cggccaggaa cgactggcag
     7201 tcgtcatcga gtataccgtc ctccctgacg ggagtgtgat gcccggcgat gtataccggg
     7261 cagtagttgc aaaccaggcg aagttagtct acgaagaggt cggcgactgg ctggagggga
     7321 gcggccccgt acccgatatg atccgggaga gaccagatct catgagacag atcctgctcc
     7381 aggatcaggc cgcaacacgg atgaagagtt atcggacgga gagaggggcg ctcgtgcttg
     7441 agaccatcga acccgaaccg ctcgtggagg atgatcgggt agtggctctt gtcatccagc
     7501 ggcagaaccg cgcccgctgt ctgatcgagg agtttatggt cgcggcgaac aggacgctaa
     7561 caggttttct gaatggcgct ggtctcccca tgatccacag ggtcgtccgc aaaccaaaat
     7621 actgggagga gatcgttgcc gtggcggcgg agcgcggcac ggcactgccc gaagagccgg
     7681 acgcggaggc gctcacccgt ttcctcatac ggcagaaagc ggccgaccca gaacgcttcc
     7741 ccgacctctc acttaccgtc ataaaactca tgggggcagg tgagtacgtg gcgttccggc
     7801 cgggagagga acctgtcggt cacttcgccc tcgctgtcac cgactacacc cacggcacag
     7861 ctccaaaccg gcgttacgtc gacatcatca tccagcggct ggtaaaatcg gtgctcgccg
     7921 gtgaacccga accgccctac gcacccgaag aactcgacga actcgcctcc tggctcacgg
     7981 gtcgggacaa ggcagcccag aaggtcgagc ggttcgtccg gaaggcggca gcggcggtcc
     8041 tcctcaggga tcggatcggt gagaccttcg aggcgtttgt caccggcgcc tcggataagg
     8101 ggacatatgt ccgcctgatc gatccgccgg ccgaaggaag ggtggtcatc ggggagcggg
     8161 ggctgcgggt tggccagagg atacgtgtcc gcctgctggc ggtcgacccc tataagggtt
     8221 acatcgattt cgggtatacc aggtgaagaa aagaaggttt agatcaccta tccagatgga
     8281 catccacctg tgggaatggg atctctatac cggcatccct gaaagccccg acgatctctc
     8341 gggtcagatc ccacttcact ccccagaggt cttccgtctt cgtccacgcc cgcagggaga
     8401 ggttcaccga ggagtccgcc agctcggtta cgaccgtcac gggttcaggt gagggaagca
     8461 ccccctcatg agacttcatc aggtcagtgg caaccctgat cgccttattc aggtcggtgt
     8521 cgtaggccac acccacagtg acatcaagac gccgggtctc catccggctg taattgatca
     8581 cggggttacc ccagacctgg gagttcggga ttgtgatgta ggtgttgtcg ggcttcagca
     8641 tctcggtcat catgattcct acacccatga ccgtcccgga tatcccgttg acctcggcaa
     8701 actccccctt atcgatgggg cggaatgccg ccacccagac ccccgcggcc agattggtga
     8761 tggtgtcctg gagccctaac cccagtataa gcccgatcac cgccgagaga ccgagaacca
     8821 cagaggagac atcaaacccc agcgtcgcca tgacgacgag tatgaggata acgtagagca
     8881 ggatcgagaa gaagcgtacc aggaactcaa cgaccaaccc ggatagtttg gatgcccggg
     8941 agagcatcgt tttgaaggct gaccggatca ccctcaccac gatcaaccct ataaccgcaa
     9001 cgatcaacgc gagaacaacg ctctcgggtg tgatactgga gagcgaagca ggcgtgctaa
     9061 gactaaaatt atctcctaac gccatacatc ctgacagatg gcatggggtg gtagatatat
     9121 ctgcaccctc cagggattcg gcgcaggctc cagagcaggg ccagctgaca tcaaaacaag
     9181 tttacagggg ggggaagtaa gtagaactgg tctcacgcca cgtgaagaaa cttgttgacc
     9241 agttcccgga tcgcttcttt gttcccgtaa agcctctcac tcagcccaag gtcatcgaag
     9301 gctttcagtg cggcgatacg gctatcgatc attcccggcg gaaagatgcc atatttctcg
     9361 aagatcgccc gtttcgcctc aagcgccccg gcagactcaa cgcatgaggc ggggagctgc
     9421 cggaattccg caagccgctc cttaaacgcc gggctgaaga tgttcccgct gacgtagagc
     9481 ctctccgcag cctcgagcgc atcgggcatc tcgatcccgt gcagcgacgc cacaatcaga
     9541 ccggcgacgg tcaggtaaag gtcggccgaa ccgtcagcaa cgcggtactc gaaggtctgt
     9601 tttgaggggc ggtcgcgaac atgacctttc tcagcggggt tggcgtcacg tgtcatgccg
     9661 tcggcaccga tccagccaag aggcaccctg atcacgaccg aacgattgcg gtcgccccag
     9721 cagaccgtcg taggtgcttc ctggtggggg acgagacgga gataggagag ggggatggtg
     9781 ttcccgaacg ccgtcagggc atcggatacg tcaagaattc cggcaatcat ctttctggca
     9841 agcgggctta acctgccctc ctcgaccaga aggttccggc cgtctttctc gacgagcatg
     9901 tggaaatgca tcccgctacc ggccttgcca acggtgatct tcggggcaaa actgatctcg
     9961 acaccgtagc ggtcccccag catccgtaaa acccatttgg caatgatcag ctgctcgacc
    10021 gcctgctcca ccggcatcgg caggaattcg atctcatgct gctcgtagta ggtgtcaccg
    10081 tcgtagaagc atccaacctc cgaatgaccg tattttaccc tgcccccggc acacgctatc
    10141 aaccgcatcg cctcatcccg caggtcagca aacttgacaa aaggcccgga tgagtgataa
    10201 cccttctgat cggtggcggg gtataggtca tcgcgcgggc tgatgacata gtactcaagc
    10261 tcgcccatgg tcatgaaggt gtaaccggtg cgacgggtga actcttcgtt cgccttgcga
    10321 agcacgtagc ctggcgaact ctccagtggt ttcccatcaa catcgtagaa agaacagagg
    10381 acctcaagcg tcggcacttc ggaaaacggg ctcacgaacg ccgtgcggta gcgcgggatc
    10441 acgtagaggt cgctgctccc cgcctcaata aaagagaaga ggttactgcc gtcgacgcgc
    10501 tcaccgtcag agaggatggt atcgaggtgg tctcgcgagg tgattacgaa gttgagggtc
    10561 tttagtttgc catcctctgc ggcgtagcgg aagttgacca tctcaacgcc gttcgcctcg
    10621 caaaaacaga taatatcctc ttttgtaaac tcagccggat ctttcttgag aaagcgtacc
    10681 agcctgttcg ggttcatcag gatctcagca tctctcattc ttttttatcg ttgtatttga
    10741 ccgtataaaa agaaatatca ttatgtgtga tacccgttta agggggttac agcccctgtt
    10801 ctgggcttga aagaacggga ctgcagccat tttcaaactc gcgcttaccg cagcagagta
    10861 gaaccccatg cggaacccgc acctggatca cttatcagag agatcccagc cgcccggcgc
    10921 cgctgctgca gtatcatcct ccagggcggg ggggctgtgg atggcgacac ctgtacggaa
    10981 tccaattggg gggaccacca gcgggcaggc gagaacagga gtcataatcc agagttcagg
    11041 cccccagcca ctcgttcata taagcgcgcg gatttagcag gtcgaaaagg gagggcggac
    11101 tatatatcac gtcaactacc ccaccctaaa gggtggggct tgctgccggg gccccttgcg
    11161 gg
//
LOCUS       DAOSZX010000045        10472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_5407_length_10472_cov_19.077566, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000045 DAOSZX010000000
VERSION     DAOSZX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_10472_cov_19.077566"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..912
                     /locus_tag="PK196_05100"
     CDS             187..912
                     /locus_tag="PK196_05100"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HON40827.1"
                     /translation="MKQVDLLYSGKAKSVYRTDDPGVYIMKFRDDITAFDGEKKDTLR
                     GKGIYNAEVSSFFFRYLEEHGIRTHYLESIEPGTMAVRALEMIPLEVIVRNIADGSLV
                     RKYPFREGEPLDPPVIIIDYKSDALHDPMLNDDLIFALRLAAPEELDLIKATALAINE
                     MLREYLDARGITLVDFKLEFGRDNGDIVVGDEISMDSMRLWDKETGASLDKDVYRFSK
                     GDVMETYAAVARRILPLPRGETA"
     gene            909..1151
                     /gene="purS"
                     /locus_tag="PK196_05105"
     CDS             909..1151
                     /gene="purS"
                     /locus_tag="PK196_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HON40828.1"
                     /translation="MKYTVTITIRLKEGMLNPEARAIQHALKNLGFQTEDLTTARLFR
                     IRLDAPDAAAARETARQMCDRLLANPVIHDYAIEVE"
     gene            1155..1886
                     /gene="purQ"
                     /locus_tag="PK196_05110"
     CDS             1155..1886
                     /gene="purQ"
                     /locus_tag="PK196_05110"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HON40829.1"
                     /translation="MRFAVVQFGGSNCDRDVCHVLTDVCGVDTDLVWYKDGLSRSYDA
                     VVLPGGFSYGDYLRPGAIAARTPIMADVIRHAKTGGLVLGICNGAQIASEAGLVPGVF
                     TLNAYPKFISRHVHLRVENTTSPFTTLYRKDEVIRIPIAHAEGRYVAPPLEIARLNRE
                     KRVAFRFCGEDGNVGPESNPNGSMESITGILSGGGNVLAMMPHPERSSEAVLGSEDGV
                     KIFNSMVAYIEEYGRRSPAQQENWA"
     gene            1883..2164
                     /locus_tag="PK196_05115"
     CDS             1883..2164
                     /locus_tag="PK196_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HON40830.1"
                     /translation="MTESNVEEEHNRAKEYAKEKGYILNPDERKLETVLRGLARNKER
                     FGAAYCPCRLRSGDPEKDRIIICPCIYHEQEIEDQGFCHCRLFFKKKSE"
     gene            complement(2386..4041)
                     /locus_tag="PK196_05120"
     CDS             complement(2386..4041)
                     /locus_tag="PK196_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HON40831.1"
                     /translation="MVTRFRRYRQIADVLVKYGFGILVEEVIPGAERLQALRKRPQEK
                     RTIYERIRLAIEELGPTYIKFGQIMSTRRELLPPELIEELQRLQDRVAPLPFEEIRPV
                     IMEYCGNLEDCFDLIEEEPAAAASLSQVHRAVLRDGHVVALKVQRPGIVDLIETDLLI
                     LQSLAARLESMFPDLQVYNAVGMVEEFAIQIRRELDFTQDGMNAERLRRNLRDVPGIR
                     IPRIYWSLSGPRMLVMDYIEGVRIDDLEAIRAMGVSPVEIAEIGFYAYVKQIFVDGFF
                     HGDPHAGNLLVTRQGEIAVLDYGIFGVIRPEKRKVFVDLLLSLSRTDVDGVLAALGGL
                     GVRIRREDVDSVKDDLYLMLLDYRETKLKRVNFAFAIRGLTDTLRRYHIRVPSSLMLI
                     MKVIVMVMDLGVRLDPDFNFDRRIRPYLTDIAARQRFSMETLEGAVRSLAGAADGLLA
                     LPGNVNEALRTLSEGTIKFEIDDRDLRQITGIVDRTSDKLLVGLVVAAIVVGSSLVIG
                     VADVPIPESISVVATLGYVVAVLIGFYAVYDAFRHRRTLKQQK"
     gene            complement(4069..>4536)
                     /locus_tag="PK196_05125"
     CDS             complement(4069..>4536)
                     /locus_tag="PK196_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40832.1"
                     /translation="VTANLLVSGMLVCGVVGQVVVGSLSDRYGRKDCTILGLMAAIPP
                     FLVFLAAGGAVSLVALMVFGFALWSTFAVSVAMAHEIAPANVGLVSGLMLGLSVGVGG
                     LGVAFTGLISDLTSLEVGLVTIPLAIVLSVLLFLAVPYPWKSLSRGGSRSPIH"
     assembly_gap    4537..4636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4637..5067)
                     /locus_tag="PK196_05130"
     CDS             complement(<4637..5067)
                     /locus_tag="PK196_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40833.1"
                     /translation="MADDTKPVWALSAAHLVTDLYSPVLPAILPLLIAEQGYSFLLAG
                     LIVTVYNLTSSFLQPVAGWLYDNKGFAVHISITVLISATFISIVGLLGNYCLIIASVA
                     IAAFGHALFHPSALGTVSRLTRDAVRGRVTSYFVIGGNLGYA"
     gene            complement(5075..5686)
                     /locus_tag="PK196_05135"
     CDS             complement(5075..5686)
                     /locus_tag="PK196_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HON40834.1"
                     /translation="MNLRRLEMELERLEGFERPTPRLEQYGTPAPVAARLLHHAAMQG
                     AIEGRRVVDLGCGTGILGCGAALLGASAVTGLDIDPAAVAAARRNAERLGLDIEFVAA
                     DVRDSGLDWGRFACDTVVMNPPFGAQKVHADRPFIDRALEIAGEVYGIFNQGSAEFVA
                     AYTEGRAVVAEVIRCGFPMRRTFAHHRRDRMDITVEVIHLKRI"
     gene            complement(5683..6630)
                     /gene="dph2"
                     /locus_tag="PK196_05140"
     CDS             complement(5683..6630)
                     /gene="dph2"
                     /locus_tag="PK196_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HON40835.1"
                     /translation="MIPISDIIEQLRGRGARRVALQFPAGLAREAPGASAALRDAGFE
                     VIVSGDPCYGGCDLALDALAYADVLVHFGHAPVEERPDVVYEPVRIDFDLSVLERVVP
                     LLKARRIGLVTTIQHVHLLDGMAAFLRGHGVEAVAAAGDGRTPVPGQVLGCNFAAARA
                     TGADEILFVGTGMFHPIGIRLSTRARVVALDPFTGQAEVVDADRLVRRRAALMEKARD
                     AANYGIILSSRSGQRRLDLARRLVGLSEKAFLVVMREVSPDALLDLGFEAYVNTACPR
                     LAYDDQVRFPVPVLAPPEFEILCGARSLDDYTIDEYLAP"
     gene            complement(6614..7165)
                     /gene="hpt"
                     /locus_tag="PK196_05145"
     CDS             complement(6614..7165)
                     /gene="hpt"
                     /locus_tag="PK196_05145"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HON40836.1"
                     /translation="MLERLIHSLETSPVMKRGDYNYFIHPITDGVPLLEPALLREIGC
                     AMVRVLDLSGVDKIVVCEAMGIHIGVTLSLMTDIPLVVVRKRSYGLPGEVAVHQTTAY
                     SKGELYLNGVGAGDRVVLIDDVCSTGGTLRALISALGQVGAGIADVCVVIGRGDADIG
                     RPCKVLVRVEVSPDRVRVVDTYL"
     gene            complement(7158..8255)
                     /gene="mfnA"
                     /locus_tag="PK196_05150"
     CDS             complement(7158..8255)
                     /gene="mfnA"
                     /locus_tag="PK196_05150"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HON40837.1"
                     /translation="MRDAGCSEEELFSFLSSKRQDDLGYRRILSSMCTPPHPVAVRAH
                     MIFIETNLGDPGLFPGTSALEDLLVERLGTLMHLPDAGGYATSGGTESNIQALRIAKK
                     LKPVDSPNVVMPASGHFSFTKACDMLGVEARTVPLDPEFRMNVEMAEDLIDANTVALV
                     GVVGTTEYGMIDPIARLSEIALDRDVFLHVDAAFGGMVAPFLDRPIPFDFRLPGVSSI
                     SVDPHKMGMSTIPAGCILVRDPDWFSRLSVDTPYLTVKREFTLAGTRPGAPVAAAVAV
                     LEYMGRDGMKAVVSGCMENCRRLIEGMETLGCPRAVTPDVNVATFRCERAPPGWRVSM
                     TRQGHMRIVCMPHVTRDVVEEFLADMCDMNA"
     gene            complement(8248..10470)
                     /gene="ppsA"
                     /locus_tag="PK196_05155"
     CDS             complement(8248..10470)
                     /gene="ppsA"
                     /locus_tag="PK196_05155"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HON40838.1"
                     /translation="MGGKGASLGEMAAIGLPVPRAFVVTAQAFRRFLVEAGIEDEIFR
                     RLERLDVDDTAALESVSREVQDIVLNAAMPDYIRQEIADAYARMDGGETVVAVRSSAT
                     AEDLPDASFAGQQETFLNIVGEEALLDAVQRCWASLYGARAIYYRAKQGFDDRSVNIA
                     VVVQELIRSEKSGVMFTSHPVTGEPLTIIEASWGLGEAIVSGSVSPDNYVFDLRTERV
                     VDRLIAEKDIMIVPDGRHGTKTIKTPPEKRLAPVLSDEEVARLAMLGKIAEDHYGVPQ
                     DIEWAIVGDEIYILQSRPITTIKQPGIRRTTETGKKSGPLGAVILQGQGASPGVASGR
                     VVIVHDVKDTSAVKEGDILVTTMTNPDMVPAMRRVNAIITDEGGMTCHAAIVSRELGT
                     PAVVGTKKATKTLKNGQIVTVDGEKGLVYEGAVEPPAVSRAAPATAAAAPVITGTLVK
                     VNVSLPEAAARAAATGADGVGLLRIEHLILGLGKTPSWYIEHDAEEEFVTELYTGIKT
                     VLDAFPGKPVWVRTLDAPTDEFRNMEGGEEEPIEHNPMLGWRGIRRDLESPDQFRLQV
                     EAFKRLWAAGYDNLGVMFPMVNHPDEFTRARAMMKDLGVDVDNVTLGIMIEIPSSAIL
                     VEDFIRAGIRFASFGTNDLIQYTLAIDRNNEHVAEMYQPEHPAVLRLIDYAISACRSH
                     GVECSICGQAGSDPRMVTWLVEHGISSVSANIDAVQRIREAVARKEKQILLEAARRNA
                     "
BASE COUNT         2048 a         3310 c         3232 g         1782 t
ORIGIN      
        1 attgtcgggg acgccgaact ccgcgaacgc ctctgcatat accctcagta catcgagagg
       61 ggctggtacc ctccgctgct cgaaccgctg atccggcacc tctctgagag gattgccgcc
      121 tcctcccggg gtgagggggc ataacctcat caagaatccc gctcaacaca tatcggagtt
      181 ctgcgcatga aacaggttga cctgctctac agtggaaagg ctaaatccgt ctaccgcacc
      241 gacgaccccg gggtctacat aatgaagttc cgggacgaca taacggcatt cgacggagag
      301 aagaaagaca cactccgcgg caaggggata tacaacgctg aagtctcgtc cttcttcttc
      361 agatacctgg aagagcacgg gatacggaca cactacctgg agagcatcga acccggcacc
      421 atggcggtgc gtgcgcttga gatgatcccg ctcgaggtga tcgtgaggaa catcgcggac
      481 gggtctctcg tgcgcaaata ccccttcagg gagggcgaac cgctcgaccc gccggtgatc
      541 atcatcgact acaagagcga cgccctccac gacccgatgc tgaacgacga cctgatattc
      601 gctctccgtc ttgccgcgcc cgaggaactc gacctgatca aggccacagc gcttgcaata
      661 aacgagatgc tccgggaata cctcgatgcg cgcgggatca cccttgttga tttcaaactc
      721 gagttcggcc gcgacaacgg tgatatcgtc gttggcgacg agatcagcat ggactcgatg
      781 cgcctctggg acaaagagac cggagcgtct ctcgataagg atgtttaccg gttcagcaag
      841 ggagacgtga tggagacgta tgccgctgtc gcaaggcgca tactccccct gccccgaggt
      901 gagactgcat gaagtatacc gttaccataa ccatccgact gaaagagggg atgctcaacc
      961 ctgaggcgcg tgccatccag cacgccctca agaacctggg gttccagact gaagacctga
     1021 ccacagcacg actcttccgg atcaggctcg atgctcctga tgcagcggcg gcccgggaga
     1081 cagcccggca gatgtgcgac cggctcctgg caaaccctgt aatccacgat tacgcgatcg
     1141 aggtcgagtg aggcatgagg tttgctgttg tgcagttcgg cggcagcaac tgcgaccggg
     1201 acgtatgcca tgtccttacc gatgtctgcg gggtcgatac cgacctcgtc tggtacaagg
     1261 acgggctcag ccgttcctac gacgccgtgg tgctgccggg cgggttctcc tacggcgatt
     1321 acctccggcc gggagcgatc gccgcgagaa ccccgatcat ggccgatgtc atcaggcacg
     1381 caaagaccgg aggcctggtg ctcggtatct gcaacggcgc ccagatagcc tcggaggccg
     1441 ggctggtgcc cggggtcttc acgctgaacg cctacccaaa gttcatatcg cggcacgtcc
     1501 acctccgcgt cgagaacacc acctcgccgt tcacgacgct ctaccggaag gatgaggtga
     1561 tccggatacc gatcgcacat gcggagggcc gttacgtcgc cccgccgctg gagattgcaa
     1621 gactgaaccg cgagaagcgg gtcgcgttca ggttctgcgg cgaagacggg aacgtggggc
     1681 cggagagcaa cccgaacggc tcgatggaga gcatcaccgg catactgtca gggggcggaa
     1741 acgtcctcgc gatgatgccg catcccgagc gctcaagcga ggcggtgctc gggtcagagg
     1801 acggggtcaa aatctttaac tcgatggtag cctatatcga agagtacggg agaagaagcc
     1861 ccgcgcaaca ggagaactgg gcatgactga aagcaatgtc gaagaggagc ataacagggc
     1921 aaaggaatac gcaaaggaaa agggctacat cttaaatccc gacgaaagga aacttgagac
     1981 cgttctccgc gggcttgcca ggaacaagga gcggttcggg gcggcatact gcccatgcag
     2041 gctccggagc ggagaccccg agaaagaccg gatcatcatc tgcccctgca tctaccacga
     2101 gcaggagata gaggatcagg ggttctgcca ctgcagactc ttcttcaaga agaagtccga
     2161 ataaccgtct tttccctggc gcggcggtgg tggagcaggg gttgccggcc agcctcccgc
     2221 ctgacagaag agatccctgt gccttccggg agtgcccttc cctggcacag acgggatgaa
     2281 acttcaggaa agtttcccac cggcagccgg gtgagagccc atcgcggaga gcgtctcgat
     2341 ggaggatcgc ccccagagat cccggacgat atcgaccctt aaaattcact tctgctgctt
     2401 tagcgtcctt ctgtgcctga aagcatcgta aaccgcgtag aacccgatca aaaccgcgac
     2461 gacatagcca agcgtcgcaa caaccgagat cgactcaggg atgggaacgt cggccacccc
     2521 tatcaccagc gacgagccca caacgatcgc tgccaccaca agcccgacga ggagtttgtc
     2581 gctcgtccgg tcgacgatgc cggtgatctg acggaggtcg cggtcatcta tctcgaactt
     2641 gatcgtcccc tcggagagcg tccgaagcgc ctcgttgacg ttgcccggga gggcaagcag
     2701 gccgtcagcc gcgcctgcaa gcgaccgcac cgcaccctcc agggtttcca tggagaaccg
     2761 ctgccttgcg gcaatatccg tcaggtatgg ccggatccgg cgatcgaagt tgaagtccgg
     2821 gtccagcctg accccgagat ccatcaccat aacgatcacc ttcattatca gcatcaggct
     2881 cgacggcacc cgtatatggt accgccggag ggtatcggtc agcccccgga tcgcgaacgc
     2941 aaagttcacc cgcttcagtt tcgtctcccg gtaatccagc agcatcaggt agaggtcgtc
     3001 ttttacgctg tctacgtcct cccgcctgat ccgaacgcca agacctccga gcgcggcaag
     3061 caccccgtca acatccgtcc tgctcaacga gaggaggaga tccacaaaga ccttcctctt
     3121 ctccggccgg atgacaccaa atataccgta atccaggaca gcgatctcgc cctgccgggt
     3181 caccagcagg ttgcccgcgt gggggtcgcc gtgaaagaac ccatcgacaa agatctgctt
     3241 gacgtaggcg taaaacccga tctcggcaat ctctacgggg gagacgccca tggcgcgtat
     3301 ggcctccagg tcatcgatcc tgacaccctc gatgtagtcc atcacaagca tccgggggcc
     3361 ggagaggctc cagtagatcc ggggtatcct gatgcccgga acgtctctca agttccgcct
     3421 tagccgctcc gcgttcatcc cgtcctgggt gaagtccagt tcacgccgga tctggattgc
     3481 aaactcctcc accatcccca cagcgttgta gacctggagg tccgggaaca tcgattccag
     3541 cctggccgca agcgactgca ggatcaggag gtcggtctcg atcaggtcga ctatcccggg
     3601 gcgctgcacc ttgagggcaa cgacatggcc gtccctcagg acagcgcggt gcacctgcga
     3661 gagggatgcc gccgctgccg gctcctcctc gatcaggtcg aaacagtcct ccaggttccc
     3721 gcagtactcc atgatcaccg gccggatctc ctcaaacggc agcggcgcaa cccggtcctg
     3781 gagcctctgc aactcctcga tcaactcggg cgggagcagt tcacgccggg tgctcatgat
     3841 ctggccgaac ttgatgtaag tcggccccag ctcctcgata gcaagccgga tacgctcgta
     3901 gatcgttcgt ttctcctggg gacgtttccg gagcgcctga agccgttcgg cccccggaat
     3961 aacctcctct accaggatcc cgaacccgta tttgaccagc acatctgcta tctggcggta
     4021 acgccggaac cgcgtgacca tcacggaagg ttctgcgctc cgggtagatt aatggattgg
     4081 tgagcgggag ccgccccgcg agagcgactt ccaggggtag gggacggcaa ggaacagcag
     4141 caccgagagg acgattgcaa gcgggatcgt cacaagcccc acctcgagcg aggtcagatc
     4201 ggagatcagg ccggtgaacg caacgccgag tccgcccaca ccgacagaga ggccgagcat
     4261 cagcccggag accaggccga cgtttgcagg ggcgatctcg tgcgccatcg ccacgctgac
     4321 cgcaaacgtc gaccagaggg cgaacccgaa gaccatcagc gcgacgagcg agaccgctcc
     4381 gccggccgcg aggaagacca ggaacggcgg gatggcggcc atgaggccga ggatggtgca
     4441 gtccttgcgg ccgtagcggt cagagagcga accgacgaca acctggccga cgaccccgca
     4501 taccagcatc cccgatacca ggaggttcgc ggtcacnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnngcgt aaccgaggtt cccgccgatc acaaagtagg aggtgacccg
     4681 gccgcggacg gcgtcccggg tcaggcggct gaccgtcccc agggcgctgg ggtggaagag
     4741 ggcgtgcccg aacgccgcga tggcgaccga ggcgatgatc aggcagtagt tcccgagcag
     4801 cccgacgatg gatatgaacg tcgcgctgat gaggaccgtg atgctgatat ggacggcaaa
     4861 ccccttgttg tcgtagagcc agcctgcaac cggctgcagg aaagacgagg tcaggttgta
     4921 gacggtgact atcagccctg caaggaggaa ggagtagccc tgctctgcga tcaggagagg
     4981 gaggattgca gggaggaccg gcgagtagag gtcggtcaca agatgggcgg cggagagcgc
     5041 ccagaccggt tttgtatcgt cagccacggt tcactcagat cctttttaaa tgtatgacct
     5101 caaccgtgat atccatgcgg tccctgcggt gatgggcaaa cgtccgcctc attggaaacc
     5161 cgcagcggat cacctccgcc acaaccgccc ggccctccgt atacgcggcc acaaactctg
     5221 ccgacccctg gttgaagatg ccgtagacct cgccggcaat ttcaagcgcc ctgtcgatga
     5281 acggccggtc ggcatgaacc ttctgcgccc caaacggcgg gttcataaca acggtatcgc
     5341 aggcaaaccg accccagtca agaccgctgt cgcgtacatc ggccgccacg aactcgatat
     5401 ccaggccgag cctctcggcg ttccgccggg ctgcggccac cgctgccggg tctatatcaa
     5461 gacctgtcac ggccgacgcc ccgaggagcg ccgcgccgca gcccaggatc cccgtcccgc
     5521 agccgaggtc gacgaccctc cgcccctcga tcgccccctg catggcggcg tggtggagga
     5581 gacgggccgc caccggcgcc ggagtcccgt actgctcaag ccgcggggtc ggccgctcga
     5641 acccctccag gcgttcgagc tccatctcaa ggcggcgcag gttcatggcg caagatactc
     5701 gtcgatcgtg taatcgtcca gcgatcgtgc gccgcagagg atctcaaact ccggcggcgc
     5761 cagcaccggg acggggaacc tgacctggtc atcgtaggca agacgcgggc atgcggtgtt
     5821 cacgtaggcc tcaaacccga gatcgaggag agcgtcgggc gagacctcgc gcataacgac
     5881 caggaacgcc ttctccgaga ggccgacgag ccgtcgggcc aggtcaagac gcctctggcc
     5941 gctcctgctg ctgaggatga tcccgtagtt tgcagcgtcc cgcgccttct ccataagagc
     6001 ggcgcggcga cggaccagac ggtcggcatc gaccacctct gcctggccgg tgaacgggtc
     6061 aagcgcgaca accctggcac gggtcgaaag ccggatgccg atggggtgaa acatccccgt
     6121 cccgacaaac aggatctcgt ccgcccccgt cgcccgtgcg gccgcgaagt tgcagccgag
     6181 cacctggccc gggaccggtg tccggccgtc ccccgcggcg gccaccgcct cgaccccgtg
     6241 cccccggagg aatgccgcca tcccgtcgag caggtggacg tgctggatgg tggtgacaag
     6301 accgatccgg cgggctttga ggagggggac aaccctctcg agcacgctga gatcgaaatc
     6361 gatccgaaca ggctcataga ccacgtcggg acgctcctcc acgggcgcgt ggccgaagtg
     6421 gaccaggaca tcggcatacg ccagggcgtc gagcgcgagg tcgcagccgc cgtaacaggg
     6481 gtccccgctg acgatcacct caaaccccgc atcccggagc gcggcagatg cccccggcgc
     6541 ctctctggca agtccggcgg gaaactggag ggcgaccctc cgggcgccgc ggccgcggag
     6601 ttgctcgatt atatcagaga taggtatcaa caacacgcac ccgatccggg gatacctcga
     6661 ccctgaccag caccttgcac ggccggccta tatccgcgtc gccccggccg atgacgacac
     6721 agacgtcggc gatcccggca cccacctgcc cgagagcgct gatgagggcg cggagcgttc
     6781 ccccggtgct gcagacgtcg tcgattagca caacccggtc gccggcgccg acaccgttca
     6841 ggtagagttc ccccttcgag taggccgtgg tctggtggac ggcgacctcg ccggggaggc
     6901 cgtaagatcg tttccggacg accaccagcg ggatatcggt cataagcgag agggtgacac
     6961 cgatatggat ccccatcgcc tcgcagacga cgatcttgtc gacccccgat aggtcaagga
     7021 cccgcaccat ggcgcacccg atctcacgaa ggagtgcggg ctcaaggagc ggcaccccgt
     7081 cggtgatcgg gtggatgaag tagttgtagt ctccccgctt catgacggga gacgtctcca
     7141 gggaatgaat caggcgttca agcattcata tcacacatat ctgccaggaa ctcttcgaca
     7201 acgtcgcgtg tgacgtgggg catgcagact atcctcatgt ggccctgccg ggtcattgag
     7261 acccgccagc ccggtggtgc acgctcgcag cggaaagtcg cgacgttgac gtccggggtc
     7321 acggcccggg ggcagccgag cgtctccatc ccctcgatca ggcgccggca gttctccatg
     7381 caccccgaga ccacggcctt catcccgtcc ctccccatgt actcgaggac ggcgaccgct
     7441 gcggccacag gggcgccggg ccgggtgccg gcaagggtga actcccgctt caccgtgagg
     7501 tagggggtat ccacgctgag gcgcgagaac cagtccgggt cgcgaaccag gatacagcct
     7561 gccgggatgg tgctcatccc catcttgtgg gggtcgacgg atatcgagga gacgccgggg
     7621 agacggaagt caaacgggat cggccggtcc aggaacgggg cgaccatccc gccaaacgcc
     7681 gcatcgacgt ggagaaagac gtcccggtca agggcgatct ccgagaggcg ggcgatgggg
     7741 tcgatcatgc cgtactcggt cgtccccacg acaccgacga gggcaacggt gtttgcgtcg
     7801 atcaggtctt cggccatctc gacgttcatc cggaactcag ggtcgagcgg caccgtccgt
     7861 gcctcaactc cgagcatgtc gcaggccttt gtaaacgaga agtggccgga tgccggcatc
     7921 acgacgttgg gggagtccac cggtttgagt ttctttgcta tccggagcgc ctggatgttt
     7981 gactccgtcc cgccgcttgt cgcgtatccc ccggcatccg ggaggtgcat cagagttccg
     8041 agccgctcga cgaggagatc ctcaagcgcg gatgtgccgg ggaagagccc ggggtcgccc
     8101 aggttcgtct cgatgaagat catgtgcgcc cgcaccgcga cagggtgggg cggtgtgcac
     8161 atcgaactca ggatacgccg gtagccaagg tcatcctgcc gctttgatga gagaaaggag
     8221 aatagttcct cctcggaaca ccctgcatca cgcatttctt ctcgctgctt caaggaggat
     8281 ctgtttttct ttacgcgcaa cggcctcacg gatccgctgg acagcatcga tgtttgccga
     8341 gacgcttgat attccgtgct cgacaagcca ggtgaccata cgggggtctg aacctgcctg
     8401 gccgcagatg gagcactcga ccccgtggct gcggcaggcg ctgatggcgt agtcgatcag
     8461 ccggaggacg gcggggtgtt ccggctggta catctcggcg acgtgctcgt tgttccggtc
     8521 gatggccagg gtgtactgga tgaggtcgtt cgtcccgaac gaggcgaacc tgatcccggc
     8581 cctgatgaag tcctcgacaa ggatggcgct gctcgggatc tcgatcatta taccgagtgt
     8641 gacgttgtcg acatcgaccc ccaggtcctt catcatagcc ctggcgcggg tgaactcgtc
     8701 ggggtggttt accatcggaa acatcacgcc aaggttgtca tacccggcgg cccagagccg
     8761 cttgaaggcc tccacctgga ggcggaactg gtctggggac tccaggtcgc ggcggatgcc
     8821 gcgccagcca agcatcgggt tgtgctcgat gggttcctcc tcgccgccct ccatgttcct
     8881 gaactcgtcg gtcggggcat caagcgtcct gacccagacg ggttttccgg ggaacgcgtc
     8941 gagaacggtc ttgatgccgg tgtagagttc ggtgacaaac tcttcctcgg cgtcatgctc
     9001 gatgtaccag gagggtgtct tgccaagccc caggatgagg tgctctatac ggaggaggcc
     9061 tacaccgtcc gcacccgtgg cggcggcacg cgctgcggcc tccggcaggg agacgttcac
     9121 cttcaccagg gtgccggtga tgacgggggc tgccgcggcc gtagccggtg cggccctgga
     9181 tactgccggc ggctccaccg ctccctcgta gaccaggccc ttctcgccgt cgacggtgac
     9241 tatctgtccg ttcttaaggg tctttgttgc cttcttcgtc cccacgaccg cgggggttcc
     9301 gagttcacgg ctcacgatgg ccgcgtgaca ggtcatgccg ccctcgtccg tgatgatggc
     9361 gttgacacgc cgcatcgcgg gcaccatgtc ggggttggtc atggtggtga caaggatgtc
     9421 accctctttg acggcgctgg tgtccttgac gtcgtgcacg atcaccaccc ggccgctcgc
     9481 gaccccgggt gaggcgccct ggccctgcag gatcacggcg ccgagagggc cgctcttctt
     9541 ccctgtctcg gtggtgcgtc ttatccccgg ctgtttgatc gttgttatcg gccgtgactg
     9601 gaggatgtag atctcatcgc cgacgatcgc ccactcgata tcctgcggga caccgtagtg
     9661 gtcttcggcg atcttgccga gcatcgcaag ccgtgccacc tcctcgtcgg aaaggacggg
     9721 ggcgagccgt ttctcgggcg gcgtcttgat ggtctttgtg ccgtgccggc cgtcgggcac
     9781 gatcatgata tccttctctg cgatcaggcg gtcgaccaca cgctccgtgc gaaggtcaaa
     9841 gacgtagttg tcgggggaga cgctgccgga gacgatggcc tcgccaagcc cccaggaggc
     9901 ctcgatgatg gtcaggggct ccccggtgac cgggtgggag gtgaacatga ccccggactt
     9961 ctcggaccgg atgagttcct ggacgacgac ggcgatgttc acgctgcgat cgtcgaatcc
    10021 ctgttttgcg cggtagtaga tggcacgcgc gccataaagc gacgcccagc accgctggac
    10081 ggcgtcaagg agcgcttcct caccgacgat gttcaggaag gtctcctgct ggccggcaaa
    10141 actggcgtcc gggaggtcct cggcggtggc gctcgaccgg acggcgacga ccgtctcccc
    10201 cccgtccatc ctggcgtagg cgtcagcgat ctcctggcgg atgtagtcgg gcatcgcggc
    10261 gttcaggacg atatcctgca cctctcgcga tacagattcc agcgcagcgg tgtcatcgac
    10321 gtccagacgc tccagcctgc gaaagatctc gtcctcgatc ccggcctcca caagaaacct
    10381 gcggaacgcc tgagcggtca ccacaaaagc ccggggcacc ggcagaccga tggctgccat
    10441 ctcacccagt gaagcccctt tcccccccac gg
//
LOCUS       DAOSZX010000046        10312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_5508_length_10312_cov_21.652725, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000046 DAOSZX010000000
VERSION     DAOSZX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_10312_cov_21.652725"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1180)
                     /locus_tag="PK196_05160"
     CDS             complement(35..1180)
                     /locus_tag="PK196_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40839.1"
                     /translation="MTFFDLAVRNIRRHWLRSSLAVIGIVIGVIAIATLGIMGNSIGL
                     MFSEMVTDVGDTLIVSPAAGVGGGKLTDRQVSDIARAVGSNVVIPFSTTADKVTVGEK
                     ESVVMIYAIPAEDIPLLLEKESGNYPRDTGGGCMIGSQVAANSRENGLKLGARAGIGG
                     DTLRVVGVLKERGLGFDINPDYAIIVPYSWYSNHYDEEDYDQVIVKVRDINDLDMVKE
                     AIEQKMNRREDVVNVMDTRGILESIFAATDAITVFLAGIGAVSLVVAGVSILNVMLMS
                     VTERTKEIGILRSIGTRRKEVMRMFIYEALILGLVGAVIGGVLSFCAGYVTTAVFVGN
                     PDYLFHPKSLLYIFFGMAFGVITSVASGLYPAWKAAHLNPIEALRYE"
     gene            complement(1177..1848)
                     /locus_tag="PK196_05165"
     CDS             complement(1177..1848)
                     /locus_tag="PK196_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40840.1"
                     /translation="MPVISFENVTKVYPLPAGDVVALDGVDIAIEEGEFVLIMGPSGS
                     GKSTLLNIMGSLDVPTSGEVTIAGRKISRMDDDDLTLLRRDHIGFVFQQFNLIPLLSV
                     VENVEYPLILKGRQNGARKRAEEVLTAVGIAATHFSHKPGELSGGQQQRAAIARALVN
                     DPDFLLCDEPTGNLDSKTGTAIMDLLSRMNREEGKTVVMVTHDIRMTEYADRTIHLED
                     GRVVA"
     gene            complement(1854..2990)
                     /locus_tag="PK196_05170"
     CDS             complement(1854..2990)
                     /locus_tag="PK196_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40841.1"
                     /translation="MKDLYLFILALCAALALASPAAAATADIAVVSSSLDPPVMISGD
                     TGTLTIVVQNNGADPVAIQSARLYGKGVVPTTDPYLAVGEIGGGNTRSFTFTVRADAG
                     EGTFYPTFVLDFRDGGSLRYPVLIQVEDTPLSISMVKKPDAFSEGRTAGITLMVGNPR
                     PNAVSGVQVVPEGAGFSVTPSSGFVGGLEPDASGTVTFNLTPETETDVTFRVVWRNGI
                     NTHTTDLVLPVTFGEDKKRANPVITNVEVTPISGGYRIVGDVMNAGLESARSVMIAPA
                     SPAIPIDPFRVYVVGTLDSDDISSFEVTLRVDAAVEDVPLIVEYRDDDGNLYTSTRTV
                     GLGGGTVAPVKEQESGGFPVLGALVVLLIALGVAGAIYHSWKRT"
     gene            complement(2992..3648)
                     /locus_tag="PK196_05175"
     CDS             complement(2992..3648)
                     /locus_tag="PK196_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HON40842.1"
                     /translation="METGFLQVLLNPARFFESRMQEEPSLKVPALIALVCGLIGAVSA
                     LMMTDITVAMLPAEMKGLGWLMVAFSTAVAFIGGFLAWIIYGFIFHIISMAFKGQGSL
                     NRTLEVTGYGLLPQVFGGIIGSIFSYLLLSGLTVPAMSSAEEIAAFSENLVTVLTTDP
                     LAQVAGIVGILFLIWSANIWIFGMKYARNLSTRDAALTVGIPVGIYILYTVITLAGWL
                     "
     gene            complement(3856..4527)
                     /gene="radC"
                     /locus_tag="PK196_05180"
     CDS             complement(3856..4527)
                     /gene="radC"
                     /locus_tag="PK196_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HON40843.1"
                     /translation="MRKRMRDTPNHDRPREKLASRGPQVLSDAELIALLLGRGTKERD
                     VWQVANDVRNYLKDAGGTPSYNDLLEIEGIGSAKACEIMACFELGRRYHAGDGVAGQR
                     VLSPEDILPLVAEWRNKKQEYFICITLNGAGEVIDRRVVTIGILNQSLVHPREVFVDA
                     ITDRAASVILVHNHPSGSLDPSTQDLGITRQLAEAGSILGIRVLDHIIVTKNGYASLK
                     ELGHL"
     gene            4689..6368
                     /locus_tag="PK196_05185"
     CDS             4689..6368
                     /locus_tag="PK196_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON40844.1"
                     /translation="MWCLISISRMELPWKSVEAWCRKGRAYAEQGQYNRAVECYDKAI
                     RQDSDRAVLWYYKGVALAAAGRDREAVECFDQAIRIDPTCARFWQAKGQAMYDLGEYG
                     EAAGSSGQAVKLAPDCTDAWLIRGHALRRIGRTLEAIECYDRVIKVEPDRVEAWLARG
                     IALASERRYDAALDCYDRVLTLDPGNVNAWYAKGTIQILLSRYEDAIISFDRAVSASP
                     GHADAWYARGCTFAALRRYEGAIASFDRALSIRPDDEEAWYNRGLALQNLERYEEALD
                     SFERAFRINPDFPGIWYRKAIALKKLKRYDLALACFDRAIRENAVDAEIWYQKGMLYT
                     LLKRYEDAIECFNQALRLKPDHLDAEYYRGESYYALGNYGSAIECYRAVVENDPKNVI
                     AWNNYGNALYRLDRYEEALICYERALEIEPDNLKVWSNKANVLSVLSHYDKALACYDR
                     ELRAHPENADAWYNKALALFILGRYSEAVTCCSHALEIDPARLEVWTTKGNALVLLER
                     YEEAVECYDRVLEVNPDDTGALKGKAAALINLDQPVEATKCYRRLQRLQRG"
     gene            complement(6635..7765)
                     /locus_tag="PK196_05190"
     CDS             complement(6635..7765)
                     /locus_tag="PK196_05190"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HON40845.1"
                     /translation="MRSLSEKVAGIAASATIEISNAAKRMAAEGIDVISLSIGEPDFD
                     TPEHIKEACIDALRRGETHYAPSTGIPELTAAIARKISTENGFSASQDEVLVTCGAKD
                     AIHQAMEAVLNPEDEALILDPSWVSYEPCVRLAGAGVRHHALDPVAFQVDDTLLEAVS
                     PRTKVIVVNTPSNPSGAVLDADSLRLIADICSDHDLYALSDEIYEKLVYGKEHISLAS
                     LPGMAERTITVNGFSKAYAMTGWRIGYAVAPRPIIRQMEKVQQHTVSHPTTFVMYGAL
                     AALRGSQDCVETMRREFERRRDYLIPALHDLGYTTAPADGAFYAYINVAGDDMAVARS
                     WLQDAHLAVTPGTAFGTPGWIRLSYATSMENLKEAVERIARV"
     gene            complement(7762..8169)
                     /gene="ribH"
                     /locus_tag="PK196_05195"
     CDS             complement(7762..8169)
                     /gene="ribH"
                     /locus_tag="PK196_05195"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HON40846.1"
                     /translation="MTIKLGFVVAEFNRDITYMMEIEAREHARFLEAEVLDTIYVPGA
                     YDMPLAIKKLLGRSDIDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFDKPVSLG
                     ISGPGMTRMEATERIDYAKRAVESAVKMVQRLA"
     gene            complement(8227..8688)
                     /gene="ribC"
                     /locus_tag="PK196_05200"
     CDS             complement(8227..8688)
                     /gene="ribC"
                     /locus_tag="PK196_05200"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HON40847.1"
                     /translation="MKIGIADTTFARVDMGGIAIDEIRKHASVGIERYVVPGIKDLPV
                     ACKKLIEERGCDLVMALGMPGSAEKDRVCAHEASQGLILCQLLTNKHIIEVFVHEDEA
                     RDAKELAWLMEQRTREHAVNAVRLALRPKDLEKLAGTGQRQGFEDVGPARP"
     gene            complement(8685..9815)
                     /locus_tag="PK196_05205"
     CDS             complement(8685..9815)
                     /locus_tag="PK196_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HON40848.1"
                     /translation="MEQEPLLMIPGPVPVPQRVRAAMTRQAINHRGPEFGAAYADTVR
                     VLKNLFGTTNDLYIISGSGTAAMEAGVANFARDRKIVSLVNGKFGDRFAKIGQRYGTV
                     TVLESEWGAPLDLAALERELEAGAEAVTMVHNETSVGIRNPAPEVGRLARKHDALFIM
                     DGITSIGGDDVRMDEWGIDVAVVGSQKCLAAPAGLAAIAVSDRAWERISDKRPFYLDM
                     AAYRKSGKATPMETPYTPAVPLFLALAEACKMIEEEGIPARIARHRRMADAVRAAAKC
                     WGVDLFPKIDEHHAYSNTATAMRIPEGVTDGDLRGTVKKFGIEIAGGQDHLKGRIFRI
                     GTMGGVGAQEILATLAAVQFALRKSGFAAGDGVEAAAGVLLG"
     gene            9951..>10312
                     /locus_tag="PK196_05210"
     CDS             9951..>10312
                     /locus_tag="PK196_05210"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HON40849.1"
                     /translation="MVDVAVICGSASDGPVAEKVFATLNEHRVSYDYRVISAHRDPEN
                     LDEYVKGSDARVFIAIAGLSAALPGVIASKTDRPVIGVPVSGKLLGLDALLSIVQMPK
                     GVPVACVGVDNGENAALLA"
BASE COUNT         2084 a         3160 c         3016 g         2052 t
ORIGIN      
        1 atcgggaaaa agagcggtgt atgggcctgg accctcactc gtagcgcagc gcctcgatag
       61 gatttaagtg cgccgccttc caggccggat agaggccgga ggccacactt gtgataaccc
      121 cgaacgccat cccgaagaag atgtagagga ggctttttgg atggaagagg taatccggat
      181 ttccgacaaa gaccgcagtc gtcacataac ccgcgcagaa actgagcacc ccgcctatga
      241 cggcgcccac aagccccagg atgagcgcct cgtagatgaa catccgcatc acctccttcc
      301 gccgggtgcc gatgcttcgc aggatgccga tctccttcgt ccgctcggtg accgacatca
      361 gcatcacgtt caggatcgag acaccagcta cgacgagcga taccgcgccg atcccggcta
      421 aaaagacggt gatggcgtct gtagccgcaa agatgctctc caggatcccc cgcgtgtcca
      481 tgacgttgac gacatcctca cgacggttca tcttctgctc gattgcctct ttgaccatat
      541 ccaggtcgtt tatatcccgg accttcacga tcacctggtc gtagtcttcc tcatcatagt
      601 ggttcgagta ccaggagtag ggcacgatga tagcgtagtc cgggttgata tcaaacccga
      661 gcccccgctc cttgagcacc cccacaacac ggagggtatc gccgccgatc ccggccctgg
      721 cgccgagttt cagaccgttc tcccggctgt ttgcggcaac ctggcttcct atcatgcatc
      781 cgccgccggt atcacggggg taattccccg attctttctc gaggaggagg gggatatcct
      841 cggcggggat ggcatagatc atcaccacac tctctttttc acccacagtg accttatcgg
      901 cggtggtcga gaagggtatg accacgttgg acccgaccgc cctggctata tcgctcacct
      961 gccgatcggt gagttttccg ccgccaacgc ctgccgccgg cgagacgatc agggtgtcgc
     1021 cgacatcggt gaccatctcg ctgaacatca gaccgatgct gttgcccatg atccccaggg
     1081 tggcgatagc gatgacgccg ataacgatcc cgattaccgc aagcgacgac ctgagccagt
     1141 gacgcctgat gttccgaacg gcgagatcaa agaaggtcat gccaccaccc tcccgtcctc
     1201 gaggtggatc gtccggtctg catactcggt catccggatg tcatgggtga ccatgacgac
     1261 ggtcttaccc tcctcgcggt tcatccggga gagtagatcc atgatcgcgg ttcctgtctt
     1321 cgagtcaagg ttcccggtcg gttcgtcgca gaggagaaag tcagggtcgt tgacgagagc
     1381 acgggcgatc gccgcgcgct gctgctgccc tccagagagt tcacccggtt tgtggctgaa
     1441 gtgggtcgct gcgatcccga ccgccgtcag aacctcttct gcccgtttcc tggcaccgtt
     1501 ctgccgtcct ttcaggatga gcgggtactc aacgttttcc acgaccgaaa gaagcgggat
     1561 caggttgaac tgctggaaga cgaacccgat atggtcacgc cgcagcagcg tcaggtcgtc
     1621 gtcgtccatc ctgctgatct ttctcccggc gatggtgacc tcgcccgagg tcgggacgtc
     1681 aagcgaaccc atgatgttca ggagggttga cttccccgac cccgaggggc ccatgatgag
     1741 gacgaactcc ccctcctcaa tggcgatatc gacaccgtca agcgccacca catcgcccgc
     1801 cgggagggga taaaccttgg tgacgttctc gaaacttatg accggcatcg tagttacgtc
     1861 cgcttccagg agtggtagat tgcccccgcg acgccgagcg cgatcaggag gacgaccaaa
     1921 gccccgagga cagggaaccc gccgctctcc tgctccttaa ccggtgccac cgtgccgccg
     1981 ccaagcccga ccgttctcgt gctggtgtag aggtttccat cgtcgtcccg gtactcgacg
     2041 atgagcggga catcctccac ggcagcatcg acccgaagcg tcacttcaaa tgatgagata
     2101 tcgtcggaat caagcgtccc gacaacgtag acgcggaacg ggtcgatcgg gatcgcaggc
     2161 gaggccgggg cgatcatcac cgaccgtgcc gactcgaggc cggcgttcat gacgtcgccc
     2221 acgatccggt aaccgccgct gataggcgtg acctcgacgt tggtgatgac cgggttggcc
     2281 cgtttcttgt cctcgccaaa cgtgaccggc agcaccaggt cagttgtgtg ggtgtttatc
     2341 ccgttgcgcc agaccacccg gaacgtgacg tccgtctccg tctccggcgt caggttgaag
     2401 gtgacggttc ctgaagcgtc aggctccagc ccgccgacaa aaccactgga gggggtgacg
     2461 gaaaaacccg ccccctccgg aaccacctgg acaccggaga cggcgttcgg ccgggggttg
     2521 ccgaccatga gagtgatccc ggccgtccgg ccttcggaaa aggcatccgg ctttttaacc
     2581 atggagatgc tgagcggggt gtcctcgacc tggatcagga ccgggtagcg taaactgcca
     2641 ccgtcgcgga agtcaaggac gaaagtggga taaaacgtcc cttccccggc gtcagcccgg
     2701 acggtgaagg tgaacgacct ggtgttccct ccaccgatct ccccgaccgc caggtagggg
     2761 tcggttgttg gcacaacacc tttgccgtag agcctggcac tctggatcgc gaccgggtcg
     2821 gcgccgttgt tctggacaac gatcgtgagc gtcccggtat ccccactaat catgaccggc
     2881 ggatcgaggg aggaggagac gacggcgata tccgcagtgg cggccgcggc aggtgaagcc
     2941 agtgccagtg cggcacagag tgccagtatg aaaagataga gatctttcat cttacagcca
     3001 tccagcaagg gttattaccg tatagaggat gtagatgccc acgggtatcc cgacggtgag
     3061 cgccgcatcc ctcgtcgaca ggttcctcgc gtatttcatg ccgaagatcc agatgtttgc
     3121 gctccagatc aggaagagga tacctactat acctgcaacc tgcgccagcg ggtcggttgt
     3181 aagaacggtc accaggttct ccgagaacgc cgcaatctct tcagcactgc tcatggccgg
     3241 gaccgtcagg cctgagagga ggaggtatga aaagatcgac ccgattatgc caccaaagac
     3301 ctgagggagg agtccataac ccgtgacctc cagagtccga ttgagtgaac cctgaccctt
     3361 gaaggccatg gagatgatat gaaagataaa accatatatg atccaggcca ggaatccgcc
     3421 tatgaaggca acggcggtgg agaacgccac catgagccat ccgagccctt tcatctctgc
     3481 gggcagcatg gcaacggtta tatcagtcat cattaaggcc gagaccgcac cgataagccc
     3541 gcagacaagc gctataagcg ccggcacctt aaggctcggc tcttcctgca tgcgggactc
     3601 aaagaaccgc gcgggattga gcagcacctg aagaaatcct gtttccattt taccacacct
     3661 ttataattct gaccgaacta aggcgataaa cctttgccgg gcgacaggaa tctcgacccc
     3721 tctatctccg gtttcagcgg cgaggccaaa ctttttgtgg ttctttcggg gagacccgct
     3781 gccagacgcc aatggttcat acgccgtatg catatgacgt tgtgttagaa aaatataacg
     3841 tttcccccac cgctgctaca ggtgaccgag ttccttcaaa ctcgcatacc cgttctttgt
     3901 gacaatgatg tggtcaagca cccggatacc caggattgac ccggcctcgg caagctgccg
     3961 cgtgatgccg agatcctgcg tggaggggtc gaggctgcct gaaggatggt tgtggaccag
     4021 gatgacggaa gccgcacggt cggtgatagc atcaacaaat acctcccgcg ggtggaccag
     4081 actctggttc aggatcccga tcgtgacaac ccggcgatcg atcacctcac cggcgccgtt
     4141 gagggtgatg cagatgaagt actcctgctt cttgttccgc cactccgcaa caagcgggag
     4201 gatatcctca ggagatagga cacgctgccc cgcaacccca tcgcccgcat gatagcggcg
     4261 cccgagctca aaacaggcca tgatctcaca ggccttcgcc gaacctatac cctctatctc
     4321 cagaaggtcg ttataggacg gggtgccgcc ggcatccttc agataattcc tgacatcgtt
     4381 tgcaacctgc cagacatccc gctcctttgt gccgcgcccg aggaggaggg caatcagctc
     4441 agcgtcgctg agcacctgcg ggccccgact ggccagtttc tctcgcgggc ggtcgtggtt
     4501 tggggtatca cgcatcctct ttctcatgct tacactgccg tatcggggtt cttctgtaac
     4561 ttgatctgga tgaaggagca tgtatatgtt gccaaatgat gcggcggacg gctcatcccc
     4621 gattccagcg ggggcgatct cgccgtcccg gatctgggga gcaaacgggt tctatcgata
     4681 tacttttcat gtggtgtttg atatccatca gcaggatgga acttccctgg aagagtgtcg
     4741 aggcctggtg caggaagggg cgcgcatacg ctgagcaggg gcagtacaac cgggcggtcg
     4801 agtgctacga taaagcgatc cgccaggaca gcgatagggc cgtcctctgg tactacaagg
     4861 gagttgccct ggctgcagcc gggcgagacc gggaagcggt agagtgtttc gaccaggcca
     4921 tcagaatcga cccgacctgc gccaggttct ggcaggccaa gggacaggcg atgtacgacc
     4981 tcggggagta cggcgaagcg gcaggttctt ccggtcaggc ggtgaaactc gcacctgact
     5041 gcaccgacgc ctggctcatc cgcggccatg ccctgcgaag gatcggccgg acactcgagg
     5101 cgatcgagtg ctacgaccgg gttataaagg tcgaacccga ccgtgttgag gcctggcttg
     5161 cccgtggaat cgcgcttgct tccgaacgcc gctacgatgc ggcgcttgac tgctatgatc
     5221 gcgtcctcac gctcgatccc ggtaacgtaa acgcctggta tgcaaaaggg acgatccaga
     5281 tcctcctctc ccgctacgaa gacgcgatca tctcattcga ccgggccgtt tccgccagcc
     5341 ctggacacgc ggacgcctgg tatgcccggg gctgcacgtt tgcagccctc cgtcgctacg
     5401 aaggggcaat cgcctccttt gatcgcgcac tcagcatccg gcccgatgat gaggaggcat
     5461 ggtataatcg tggcctggcg ctccagaacc tggagcgcta tgaggaggcg ctcgatagtt
     5521 tcgaacgcgc cttccggatc aacccagatt tccccgggat ctggtaccgc aaggccatcg
     5581 cccttaagaa actgaaacgc tacgaccttg cgcttgcctg ctttgatcgg gcgatccggg
     5641 agaacgccgt cgatgcagag atctggtacc agaaaggaat gctctacact ctcctgaaac
     5701 gttacgagga tgcaatagag tgtttcaacc aggctctcag gctcaagccc gatcatctcg
     5761 acgccgaata ctaccgcggc gagagttact acgccctcgg gaactacggg tcagcgatcg
     5821 agtgctaccg cgccgtcgtc gagaacgacc caaagaacgt catcgcctgg aacaactacg
     5881 gcaacgcatt atatcgcctc gatcgttacg aggaggcgct catctgctat gaacgcgccc
     5941 tggagattga acccgataac ctgaaggtct ggagcaacaa ggcaaacgtt ctctcggtcc
     6001 tctcacacta cgacaaagcc ctcgcctgct acgacaggga actgcgggcc cacccggaga
     6061 acgctgacgc ctggtacaac aaggccctcg cgctcttcat tctggggcgc tacagcgagg
     6121 cggtcacctg ctgctcgcac gcgctggaga tcgatcccgc aaggctcgag gtctggacaa
     6181 cgaagggcaa cgctctcgtg ctcctggaac gctatgagga ggcggtcgag tgctacgacc
     6241 gggttctgga ggttaaccct gacgataccg gggcgctcaa aggtaaagcg gcggctctca
     6301 tcaaccttga ccaacctgtc gaagcaacca agtgctacag gaggctgcag cggttgcagc
     6361 ggggttaacc ggtgctaacc tgggaggagg ccgtcgccgg caacctctcg atccgggacg
     6421 cttttacagc ctggtcttca gcgagcagga taatgcacgg ctgctcgcac tccgggcata
     6481 tccctgctgc ggccccctat atagcatttc tgtcacaatc ctctacacgt ccgggtaccg
     6541 ggaccgattc tgaaccttcg ccgccgactg ataacggagc ggttcatcgt gaaggcttct
     6601 cgaacagaga tctaaagaaa aatgggcggg ctcgctaaac ccgcgctatc cgctcaaccg
     6661 cctccttgag gttctccatc gaggtggcgt aggagagcct gatccagcct ggtgtgccaa
     6721 acgcggtgcc gggcgtgacg gccaggtggg cgtcctggag ccatgaccgg gcgactgcca
     6781 tatcgtcgcc agccacattg atgtaggcgt agaacgcccc gtctgcgggg gcggtggtat
     6841 agccaaggtc gtgcagcgcc gggatgagat aatcgcggcg ccgctcaaac tcacggcgca
     6901 tcgtctccac acaatcctgg ctgccccgga gggcagcgag ggcgccgtac atcacaaacg
     6961 tcgtcgggtg cgatacggtg tgctgctgca ccttctccat ctgccggatg atcggccggg
     7021 gagcgacggc gtagcctatc cgccagccgg tcatcgcgta ggccttcgag aacccgttga
     7081 ccgtgatcgt ccgctccgcc atacctggaa gtgaggcgag ggagatgtgc tccttcccgt
     7141 agaccagttt ctcatagatc tcatcggaga gcgcatacag gtcatgatca ctgcagatat
     7201 cggcgataag ccggagagag tccgcatcaa gcaccgcacc cgaagggttc gagggtgtat
     7261 tgacaacgat cacctttgtc cggggactga cggcctcaag gagtgtgtcg tcgacctgga
     7321 aggcaacagg gtcaagcgcg tggtgccgga caccggcgcc agcaagcctg acgcagggtt
     7381 cgtaggagac ccatgaaggg tccagtatga gcgcctcgtc ctccgggttc agcaccgcct
     7441 ccatcgcctg gtggatggcg tcttttgccc cgcaggtgac cagaacctca tcctgagatg
     7501 cggaaaaccc gttctcggtg gagattttcc gggctatcgc ggccgtcaac tcaggtatac
     7561 cggtgctcgg ggcgtagtgg gtctcccccc ggcggagggc atcgatgcag gcctccttta
     7621 tgtgctccgg cgtatcgaag tccggttccc cgatcgagag actgatgaca tcgataccct
     7681 ccgcggccat ccgctttgca gcgttcgaga tctcgatcgt tgcagaggcg gctatacccg
     7741 caaccttctc cgataggctc ctcatgccaa tcgctggacc atcttcacgg ccgactcaac
     7801 ggcgcgcttt gcgtaatcga tccgttcggt tgcttccata cgggtcatcc caggcccgga
     7861 gatgccgaga gagaccggtt tatcgaactc aagggagagg tcgatgatct ttcgcgcagc
     7921 atgctggacg acgatctcgt cgtgctgggt ggcgccctcg atgacacagc ctatggtgac
     7981 aaccgcatcg atatcgctgc gcccaagcaa cttcttgatc gcaagcggca tgtcgtatgc
     8041 cccggggacg tagatcgtat cgagaacctc cgcctcaagg aacctggcgt gctctctggc
     8101 ctctatctcc atcatgtagg tgatgtcgcg gttgaactcc gcgacgacaa atccaagttt
     8161 tatcgtcatc tcaatactcc tgtgttagtt ctatagcacc gttcgttggc aacttatcaa
     8221 ttcaaatcat gggcgtgcag gcccaacatc ctcgaaaccc tgccgctggc ctgttccggc
     8281 aagtttctcg agatcttttg ggcggagggc aagccgaacg gcgttcaccg catgctctct
     8341 cgtccgctgc tccatcagcc atgccagttc ctttgcgtct cttgcctcgt cctcgtggac
     8401 gaagacctcg attatgtgtt tgtttgtcag gagctggcag aggataaggc cctgtgaggc
     8461 ctcgtgagca cagaccctgt ccttctccgc actcccgggc atcccgagcg ccatcaccag
     8521 gtcgcacccg cgctcctcga tcagtttttt gcaggccacc ggaaggtcct tgatcccggg
     8581 gacgacgtat cgctcaatcc cgacgcttgc gtgcttccgg atctcatcta tggcgatccc
     8641 gcccatatcc acccgggcaa acgtggtatc ggcgatcccg atcttcatcc cagcagcacc
     8701 ccggcagccg cttccactcc gtcaccggcc gcaaaccctg actttctgag ggcaaactgg
     8761 accgcggcaa gggtagccag gatctcctgc gccccgacac cgcccatggt gccgatgcgg
     8821 aagatcctgc ccttgaggtg atcctggcca ccggcgatct cgatgccgaa cttcttgacg
     8881 gttccccgga gatctccgtc ggtgacacct tcagggatcc gcatggcagt ggcggtgttt
     8941 gaataggcgt ggtgttcgtc gatcttcggg aagaggtcga cgccccagca cttcgccgcg
     9001 gcacggacag cgtcggccat ccggcggtgg cgggcaatac gggcaggaat gccttcctcc
     9061 tcgatcatct tgcatgcctc agcaagcgca aggaatagcg ggacggccgg ggtgtagggt
     9121 gtctccatcg gggtggcctt cccgctcttg cgataggcgg ccatatccag gtagaacggc
     9181 ctcttgtccg agatgcgttc ccatgcgcgg tcgctcacgg cgatggcggc aagacccgcc
     9241 ggggcagcaa gacatttctg cgacccgaca accgctacgt caatacccca ttcatccatt
     9301 cggacgtcgt cgccaccgat ggaggttatg ccatccatga tgaagagggc gtcgtgcttc
     9361 cgggctagtc tgccaacctc gggtgcggga ttcctgatcc cgacgctcgt ctcgttgtga
     9421 accatcgtaa cagcctcagc gccggcctca agttcccgct cgagggccgc gaggtcgagc
     9481 ggcgcccccc attcggactc gagcacagtc acggtgccgt aacgctgccc gatctttgcg
     9541 aaacgatcgc cgaacttgcc gttgacgagc gagacgatct ttctatcccg cgcgaagttt
     9601 gcaacaccgg cttccatcgc cgcggttccc gaaccgctga tgatgtaaag gtcgtttgtg
     9661 gtgccaaaga ggttctttaa cacgcgcaca gtatccgcat acgccgcccc gaactcgggg
     9721 ccacggtggt tgatggcctg ccgcgtcatg gcggcgcgca cccgctgcgg gacgggcacc
     9781 ggcccgggga tcataaggag tggttcttgt tccatgagat atcagtccgt attgtctcgt
     9841 tctggacgga cgcttttcct ccaaggaagt tggatgtggt ttcatatcaa acccaccacc
     9901 gtgcaatacc gacagggaga aaaagatgcg ggggcataat gaaatgaggt atggttgacg
     9961 tcgcggtgat ctgcggttcc gcctccgacg gccccgtcgc ggagaaggta tttgcaaccc
    10021 tgaatgaaca cagagtctcc tacgactacc gggtgatctc agcgcaccgt gacccggaga
    10081 atctggacga gtacgtgaag gggagtgatg cccgggtctt catcgccatc gccggacttt
    10141 cggcggccct gccaggggtg atagcatcaa agaccgatcg cccggtgatc ggtgttcctg
    10201 tgagcgggaa acttctgggt cttgatgccc tcctctcgat cgtccagatg ccaaaaggcg
    10261 tcccggtggc ctgcgtcggt gtggacaacg gggagaacgc cgctcttctc gc
//
LOCUS       DAOSZX010000047        10246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_5559_length_10246_cov_18.568737, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000047 DAOSZX010000000
VERSION     DAOSZX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5559_length_10246_cov_18.568737"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..721
                     /locus_tag="PK196_05215"
     CDS             365..721
                     /locus_tag="PK196_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HON40850.1"
                     /translation="MITDREKAAFEAGIKLGALYHQFVGTPISRETADSVEAAIEQAV
                     SLQPCVTDVRVRLNRGMMVSNRFGYSELAGKMFDAEVTTRVGTAVCRARLHLQGDYPM
                     MEIIETHETARDTDHR"
     gene            690..1520
                     /locus_tag="PK196_05220"
     CDS             690..1520
                     /locus_tag="PK196_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HON40851.1"
                     /translation="MKPPAIPITDDHIHIDPLNGRGIEAAKDFRRSGGTHIFLVTKPS
                     WSSGVEPSSGEDYREVFERTLRVADDIREIGVVVYPVLGVHPAEISRLAGRMGLEEAA
                     GVMMAGLDLAAAYVQEGAAVALKSGRPHYETAPEVLAASNAVLYHALELGADCGCAVQ
                     LHAESGPCIDVVPMAKRAGIPIERVVKHFATPDTPLVPSLIARHEGIPALARAGLRFS
                     MESDYMDENARPGAVIGPKSVPRFTRRYLEEGLITEEDAWQIHAGTPSRTYGVEISLP
                     "
     gene            1567..1860
                     /locus_tag="PK196_05225"
     CDS             1567..1860
                     /locus_tag="PK196_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HON40852.1"
                     /translation="MYLKVHHIPGSGEVVAACDTDLMEVTLMHGEVEVRISGGFYGTQ
                     TATEDEVRGALADADNANIIGKEVVALAVSMGLIAEKDCIMIDGVPHAQIFRI"
     gene            1864..2910
                     /locus_tag="PK196_05230"
     CDS             1864..2910
                     /locus_tag="PK196_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HON40853.1"
                     /translation="MTAQTSICPRCGRPSEDGGICPACRAAEVRLLSCKPYVTAVYCP
                     VCGSQKRGSTWSDLEVSREDLIAELAISAVTIHQDARDARVTIQSEDPAPNRTACTIE
                     VEAALYGVPVRETCRTEIRWQKESCDRCSRISGGYYAGIVQVRATDRKINAYEREVAT
                     NIAEQAEESLQQSGDRLSFISDLDDRKDGLDITVGTQHLGQEIAKQVTGALGGRFTTH
                     PKLVGEKDGKGLYRITYSIRLPYYQKGDVVVSKGRYYEVREIEGQRLRVFDLQDGTPL
                     TLRTDDAERLIGNVREARSALVVFTQPGVVGLMDPETYVTREVNPVPWTTPVEGGCIR
                     VLRDDEDDRLVIVG"
     gene            2917..3786
                     /locus_tag="PK196_05235"
     CDS             2917..3786
                     /locus_tag="PK196_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HON40854.1"
                     /translation="MRVRRLPASVLPRIAGEAWVDPDRRAYLRDGIACVPVRDGCPAD
                     EDLPERRVYRGRGYHLIGDVAVVHGDAPTKEEIEAIVAHRHPRGVVWLKGCTGAMRIP
                     DAEVVYGSAGEVRHREQGCTFILDPTRVMFAQGNRVEKARIAALVRPGERIADMFAGI
                     GYFTIPAARSGASIHAMEINPVAFDYLRRGIAANSVADRVTPMMGDCRGLLSGVYDRI
                     LMGHFEAASFLPDALAHVRPGSVIHVHSIGGATAAIQERLMEAGLSAAISSRRVKKYG
                     PNAWHMVQDVRIE"
     gene            3783..4916
                     /gene="coaBC"
                     /locus_tag="PK196_05240"
     CDS             3783..4916
                     /gene="coaBC"
                     /locus_tag="PK196_05240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HON40855.1"
                     /translation="MKTLSGKTVVLAVTGSIAAVETVKLAHALRRRGAAVQAVMTEAA
                     RGIIHPDALTYATGRPAITRITGLVEHVLYCGEGGEADLLLVAPCTANTIGKIAAGID
                     DTPVTTFASTALGRGMPVVVVPAMHESIYRHPGVVENIERLRSWGIDLVEPRIEEGKA
                     KIADTDTIVLHAERAVSGRPLAGKRVLITSGACAEPLDDVRVLTTRSTGLMGRALALE
                     AFRLGADVAVVHAGSFPCVRNIRAATAAEMRQAVLSVCRDEGVDVYISAAAISDYAPE
                     RHEGKIRGGSPMTIRLNPLPKVIDEVMQTCRPAVTVAFKLGWDEEERARAMLDAGVRM
                     VVLNTPATMGAAEGSFVIMTAGGSRNVTGSKEEVAGAIWSELL"
     gene            4898..5740
                     /locus_tag="PK196_05245"
     CDS             4898..5740
                     /locus_tag="PK196_05245"
                     /EC_number="2.7.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HON40856.1"
                     /translation="MVRTAVAFSPGHISGYFRRIEGRDTATTGSIGAGVVIDEGVRST
                     VEEAGETSVRVLRDGCVSTGSPPIEYALDRIGVCARVTTECRLPIGAGFGLSAAALLA
                     TLTAANSLFDLGLSADGVAAAAHEAEVVQRTGLGDVAASLGGGVACRKGPGITAEVAR
                     FYPEEPIYAMSLGPLPTASVLASAETMARIAAAYPRRCPHNLVDFYRLSRSFAERSRL
                     IAPDLRRVLEACDAAGVPASMTMLGNGVFASGAAAEEVLARFGEVYTLAVAHRGPYLI
                     EVRP"
     gene            5737..6468
                     /locus_tag="PK196_05250"
     CDS             5737..6468
                     /locus_tag="PK196_05250"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HON40857.1"
                     /translation="MIPKDHPRYRSLVARERLARCAREGVVALEGLAAHGRGEAFDYL
                     IGERTTPSAAVAARTAAAMLLSARHAVISVNGNTAALAADEVAALQRASGADVEVNLF
                     HRTGDRVERITRLLESHGVRVLRGTAERLIPLSHDRALCLPGGIGDADLLLVPLEDGD
                     RCAALRRMGKTVITIDLNPLSRTARTANLTIVDELTRALPGITAGCSSLEPGEAERLI
                     GSLDNAYLLRAAIDEIRENLSHALE"
     gene            6455..7468
                     /locus_tag="PK196_05255"
     CDS             6455..7468
                     /locus_tag="PK196_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON40858.1"
                     /translation="MLWSEVYRPRRCEDIIGQDAVIRQMTAFADSGSIPHMLIFGPHG
                     TGKTTAVGCMTERLYGENREANTTVFSTADLLGKGKSVLESDDRFSHIYRKDLSLIAN
                     FKRIVKWYASMRPLDAEFKVMVFEDAEHLTFEAQQSLRRTMERYSATCRFVFVTVRPS
                     AIIPAIASRCLPLFFAPLDAALVRARLEEILAAEGASVPKDDLDLIVCAAHGDLRRAI
                     TYLQIAAGSGEEGFDLAEVARSETGNIAASAFEALRGGNLEAARGIVESLLIDYGLSA
                     QEAVVELREAIRRDYNHPALVIALADTDLRLTHSANDFVQLNALLARIALEVSGEEGT
                     AAL"
     gene            7443..8159
                     /locus_tag="PK196_05260"
     CDS             7443..8159
                     /locus_tag="PK196_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HON40859.1"
                     /translation="MKKVQQHYDEIADVYDERYDGRRGRCYHGHICDHVMRNLPKGGF
                     LLDVGCGTGLFVQRYVEEGGRAVGLDVSPGMVRHGRGRCPDCGFCVGTADALPFEDGS
                     FDALASLLAFSYIPDPEATLRECYRVLAPGGRIAVCTLSRTVFTSIVPFVYRMSERIG
                     LRGVGMGDFDEHYYTNAEMARLFCDAGFEDVTISRCSFAHLKLIEPIFTLARKVEPFI
                     EKRLPYLAYNVCASGKKPEN"
     gene            8357..8896
                     /locus_tag="PK196_05265"
     CDS             8357..8896
                     /locus_tag="PK196_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON40860.1"
                     /translation="MEPVDIVLTKETFEVLASGTRLTILKNLHERRMTITELADGLGL
                     AKSTVHHHLQRLADAGLVAADDDGRAWVYYALTPEGRALLQPHGSARIRIILTAGLAS
                     LAGGVVAVAAFLTAPIREVPVPPIAGGGGLPVPEGAPTELLAAGIALAVIGVILIAYA
                     CARRRGASPRTRGSRDLYR"
     gene            9015..10235
                     /locus_tag="PK196_05270"
     CDS             9015..10235
                     /locus_tag="PK196_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HON40861.1"
                     /translation="MKKLLIFSGVCVLVLALIAAGLSAAGTTRPDTPDGAGGVAAGNN
                     LFAFDLYRHLAADPAYAGENLFFSPYSIASALAITCEGARGTTADEIESVLHLPTNET
                     LRREGFADLNAALNSGSGNCTLRTANALWAEKTHSLLPGYIETAARWYGANATNLDFI
                     ENPEGSRETINRWVEEKTEDRIRDLLPPGSIDPLTRLVITNAIYFKGAWAQQFDPART
                     TEEEFRVSPNETVAVPMMHGNAVYPYAETGTLQVLEMPYFHGNGTELSMLVLLPKEDS
                     LTAAEEALDADLRESLAPQNVRVFFPKFTLETEYPLPGTLAAMGMPTAFTGDADLSGM
                     DGTRDLFVTGVFHKAFIDVNEEGTEAAAATGVAAGKGVTPVFRADHPFVFLITEKDSG
                     AILFMGRVVNPESP"
BASE COUNT         1875 a         3186 c         3439 g         1746 t
ORIGIN      
        1 gagcagggaa gggatttcgg cagggtgttc acatcgagcc gcggatctgc tcatcctgtc
       61 catggaactg tgtcgatggg gaaggctgca ccgacaaccc acaatggacg ccctgaccaa
      121 aaacctccac gataatgcac catcactccg tgattcggcc ttcatcaggg tcagggaatg
      181 ggaggatccc gccgtcatac atcccccgag gattacgctc gctacgcctc cagatggcca
      241 ccgtctgaaa cgaggcctgc agccactgca caacggtcag caagacgctt gcgcttacgc
      301 acgcaacggc acatccctgc ccccggggcg caccttctta ctcccacaac cccaacatac
      361 actcatgatc accgaccgcg agaaagcggc gtttgaagcc gggatcaagc tcggcgccct
      421 ctaccaccag ttcgtcggga caccgatctc ccgcgagacc gccgattccg ttgaggccgc
      481 catcgagcag gcggtcagtc tccagccctg cgtgacggat gtccgcgtcc ggttgaaccg
      541 cgggatgatg gtctcaaacc ggttcggcta ctcggaactt gccgggaaga tgtttgatgc
      601 ggaggtcacg acccgggtcg ggacggccgt ctgccgcgcc cggctgcacc tccagggcga
      661 ctaccccatg atggagatca tagagaccca tgaaaccgcc cgcgataccg atcaccgatg
      721 accatatcca tatcgacccc ctgaacggcc gggggatcga ggccgcgaag gacttccgcc
      781 gcagcggcgg gacacacatc ttcctggtca caaaaccctc atggtcgtct ggcgtagaac
      841 cctcctcggg agaggactac cgcgaggtct tcgagagaac cctccgggtt gctgacgata
      901 tccgggagat aggggttgtg gtctaccccg tccttggcgt ccacccggca gagatcagcc
      961 gtcttgccgg gaggatgggg cttgaggagg ccgccggcgt gatgatggcc gggctcgacc
     1021 ttgccgccgc ctacgtccag gaaggggcgg ccgtggcgct caagagcggc cggccccact
     1081 acgagactgc accagaggtg cttgcagcct caaacgccgt cctctaccac gcgcttgaac
     1141 tcggcgccga ctgcgggtgc gccgtccagc tccatgccga gagcgggccg tgcatcgacg
     1201 ttgtcccgat ggccaagcgg gccggtatcc cgatcgagcg ggtcgtcaaa cactttgcaa
     1261 cccccgacac accgctcgtg ccgtccctga tcgcgcggca cgagggtatc ccggcgctcg
     1321 cccgtgcagg gctgcggttc agcatggaga gcgactacat ggacgagaac gccaggcccg
     1381 gcgctgttat agggccaaag tcggtcccgc ggttcacccg gcgctacctt gaagaggggc
     1441 tcatcaccga ggaggatgcc tggcagatac acgccgggac gccgtcacgc acatacggcg
     1501 tcgagatctc gctcccctga ccggaacggc gcaccttttt gggatcttcc gatcaaacaa
     1561 tctacaatgt acttaaaagt gcaccacata cccggctccg gcgaggtggt ggccgcctgc
     1621 gacaccgacc tgatggaggt caccctgatg cacggcgagg tggaggtccg catcagcggc
     1681 ggattctacg gcacacagac cgccacggag gatgaggtgc ggggggcgct tgccgacgcc
     1741 gacaacgcca acatcatcgg caaagaggtc gttgccctgg ccgtctccat ggggctcatc
     1801 gccgagaagg actgcatcat gatagacggc gtcccccacg cccagatatt caggatctaa
     1861 ggtatgaccg cccagacaag catctgcccc cgctgcggga gaccgagcga agatggaggg
     1921 atctgccctg cgtgccgggc ggcagaggtg aggctgctct cctgcaaacc ctatgtgacc
     1981 gccgtctact gtccggtctg cgggtcgcag aaacgcggca gcacctggtc agaccttgag
     2041 gtctctcgcg aagacctcat cgccgaactt gccatatcgg cggtgaccat ccaccaggac
     2101 gcaagggatg cccgtgtcac catccagtct gaggatccag ccccaaacag gacagcctgc
     2161 accatcgagg tggaggcggc gctctacggc gtcccggtga gggagacctg cagaacagag
     2221 atccgctggc agaaagagtc ctgcgaccgc tgcagccgca tctccggcgg ctactacgcc
     2281 ggtatagtcc aggtgcgggc aaccgaccga aagatcaacg cctacgagcg tgaggtcgcc
     2341 acaaacatag cggaacaggc ggaggagtcg ctccagcagt cgggcgaccg cctctcgttc
     2401 atatccgacc tcgacgacag aaaagacggt ctcgacatca ccgtcgggac acagcacctc
     2461 ggccaggaga tagcaaaaca ggtcacggga gcgctcggcg gccggttcac gacccacccg
     2521 aaactggtcg gggagaagga cgggaagggg ctgtaccgga tcacatactc catccgtctc
     2581 ccctactacc agaagggcga cgtggttgta tcgaagggca gatactacga ggtgcgcgag
     2641 atcgaggggc agcggctcag ggtgtttgac ctccaggacg gcacgccgct gacgctcagg
     2701 accgacgatg cagaacgcct cataggcaac gtccgtgagg ccaggtcggc tctcgtcgtc
     2761 ttcacccagc caggtgtcgt ggggctgatg gacccggaga cctacgtgac tcgcgaggtc
     2821 aacccggtgc cctggacgac gccggtggag gggggctgca tccgggtgct ccgcgacgac
     2881 gaagacgacc ggctggttat cgtggggtga tgggctgtgc gggtgcgaag gctgccggca
     2941 tcggttctgc cgcgtatcgc gggcgaggcg tgggtcgatc ccgaccgccg ggcatacctg
     3001 cgtgacggga tcgcctgcgt cccggtcagg gatggctgcc cggcggatga ggatctcccg
     3061 gaacggaggg tctaccgcgg ccggggctac cacctcatcg gcgatgtcgc ggtcgtccac
     3121 ggcgatgcgc ctacgaaaga ggagatcgag gcaatcgtgg cgcaccgcca cccccgcggt
     3181 gtcgtatggc tgaagggctg caccggtgcc atgcggatcc cggatgcgga ggtggtctac
     3241 gggagcgccg gggaggtgcg gcaccgggag cagggctgca cattcatcct tgaccctaca
     3301 cgggttatgt ttgctcaagg aaaccgcgtg gagaaggcaa ggatcgccgc tcttgtccgg
     3361 ccgggggagc gcatcgccga tatgtttgcc gggatcggct acttcaccat cccggcggcg
     3421 cgcagcggtg caagcatcca tgcgatggag ataaaccccg tagcctttga ctacctgagg
     3481 cgcggcatcg cggcaaacag cgtcgccgac cgggtcacgc cgatgatggg cgactgccgc
     3541 ggcctcctct cgggggtcta cgaccgcata cttatggggc atttcgaggc ggcatcgttc
     3601 ctccccgacg ccctcgccca tgtccggccg ggaagcgtca tccacgtcca cagcatcggg
     3661 ggcgcgacgg cggcgatcca ggaacggctg atggaggccg ggctatcggc ggcgatatcc
     3721 tcgcgccggg tgaagaaata cgggccgaac gcctggcata tggtgcagga tgtgaggata
     3781 gaatgaagac actttcagga aagaccgtgg tgctcgccgt gaccggaagc atcgcggcgg
     3841 tcgagacggt gaaacttgcc cacgccctgc gccgccgggg ggcagcggtg caggcggtaa
     3901 tgacggaggc cgcgagaggt atcatccatc ccgacgccct gacctacgcg acagggcggc
     3961 cggcgatcac ccggatcacc gggctggtgg agcatgtcct ctactgcggc gagggcgggg
     4021 aggccgacct cctgctggtt gcaccctgca ccgcaaacac gattggaaag atcgctgccg
     4081 ggatcgatga caccccggtc acgacgtttg cctcgaccgc gctcggccgc gggatgccgg
     4141 tggtggtcgt cccggcgatg cacgagagca tctaccgcca tccgggggtc gtggagaaca
     4201 ttgaacggct ccgttcatgg gggatcgatc tcgtcgagcc ccggatcgag gaggggaagg
     4261 ctaagatcgc cgataccgat acgatcgtgc ttcacgccga acgtgccgtc tccgggagac
     4321 cgcttgcagg aaagagggtg ctgatcacga gcggggcgtg cgctgaacct ctcgacgatg
     4381 tccgggttct gacgaccagg tcgaccgggc tgatggggcg ggcgcttgct ctcgaggcgt
     4441 tccgcctggg ggcggatgtt gctgttgtcc acgcggggtc gtttccatgc gttcgaaaca
     4501 tccgggcggc aacggccgcg gagatgcgcc aggccgtcct ctcggtctgc cgggacgagg
     4561 gggtcgatgt gtatatcagc gcagcggcga tatcggatta tgctcccgag aggcatgagg
     4621 ggaagatcag gggcggatct ccgatgacga tccggctgaa cccgctcccg aaggtcatcg
     4681 atgaggtcat gcagacctgc cgcccggcgg tgacggtggc cttcaaactg ggctgggacg
     4741 aggaggagcg ggcgagggcg atgctcgacg ccggtgtgag gatggttgtt ctgaacaccc
     4801 cggcaacgat gggagcagcg gaggggtcgt ttgtgatcat gactgcaggg gggtcgcgga
     4861 acgtgaccgg gagcaaagag gaggtcgccg gggctatatg gtcagaactg ctgtagcatt
     4921 ctcgccgggt catatatccg gatacttcag gcgcatcgag ggtagagata ccgcgaccac
     4981 agggagcatc ggcgccggcg ttgtgatcga tgagggtgtg cgctcgacgg tcgaggaggc
     5041 gggggagacg agcgtccggg ttctccggga tggctgcgtg agcaccgggt cgccgccgat
     5101 cgagtacgcc cttgaccgga tcggggtctg cgcccgcgtc acgacagagt gccggcttcc
     5161 cataggcgcc gggttcgggc tctcggcggc ggcgctactt gcgacgctca cggccgcaaa
     5221 cagtctattt gatctggggc tctccgcaga cggggttgcg gccgccgccc acgaggccga
     5281 ggttgtccag cgcaccggtc ttggggacgt tgccgcaagc ctcggcggcg gggtcgcctg
     5341 ccggaaaggg cccgggatca ctgcagaggt tgcccgattc tacccggaag aacccatcta
     5401 cgccatgagt cttgggccgc tgccgacagc ctccgtcctt gcatcggctg agacgatggc
     5461 acggatcgct gccgcgtacc cccggcgatg tccgcacaac ctggtcgact tctaccggct
     5521 ctcgcggtcg tttgccgaga ggagcaggct catcgcgccg gatctccgcc gggtgctgga
     5581 ggcctgcgac gccgccggcg tcccggcaag catgacgatg cttggaaacg gtgtctttgc
     5641 cagcggtgct gcggcggagg aggtgctcgc ccggttcggg gaggtctaca ccctggcggt
     5701 cgcacaccgc ggcccatacc tgatcgaggt gagaccgtga tacctaaaga ccatccacgc
     5761 taccgctccc tcgtcgcccg cgaacgcctg gcccgctgcg cccgcgaggg tgtagtcgcc
     5821 ctggaagggc ttgccgccca cggccgcggg gaggcgtttg actaccttat aggcgagagg
     5881 acgaccccga gcgcggccgt tgccgccagg acggccgctg cgatgctcct ttccgcccgg
     5941 cacgcggtta tatcggtgaa cgggaacacc gcggcgcttg cagcggacga ggtcgcggcg
     6001 ctccagaggg cgagcggcgc cgatgtcgag gtgaacctct tccaccggac gggggaccgg
     6061 gtggaacgga tcacccggct ccttgaatcc cacggtgtcc gggttctcag ggggacggct
     6121 gaacgactca taccgctctc ccacgaccgc gccctctgcc tgccgggagg gataggggac
     6181 gccgatcttc ttcttgtgcc gcttgaggac ggcgaccgct gtgcggcgct ccggcgtatg
     6241 ggaaagaccg tcataacaat cgatctaaac ccgctgtccc ggacggcccg aaccgcgaac
     6301 ctgacgatcg tcgacgagtt gacccgtgcc ctccccggga tcaccgcggg ctgttcaagc
     6361 cttgagcccg gggaggctga acgcctgatc gggtcgctgg acaatgccta tcttctccgg
     6421 gcggcaatag atgagatcag agagaacctg agtcatgctc tggagtgagg tctaccgccc
     6481 ccggcggtgc gaggatatca tagggcagga tgcggtcatc cggcagatga cggcgttcgc
     6541 ggactccggc agcatacccc acatgctcat cttcgggccg cacgggacag ggaagaccac
     6601 cgccgtcggg tgtatgacag aacggctcta cggcgagaac cgggaggcga acacgacagt
     6661 tttcagcacg gccgacctcc tggggaaggg gaagagcgtc cttgagtcgg atgacaggtt
     6721 cagccatata taccggaaag acctgagcct gatcgccaac ttcaagcgga ttgtgaagtg
     6781 gtatgcctcg atgcggccgc tggatgccga gttcaaggtg atggtctttg aggacgccga
     6841 acacctgacg tttgaggcgc agcagtcgct ccgccggacg atggagcgct acagcgccac
     6901 atgccggttc gtctttgtca cggtgaggcc gtcggccatc ataccggcaa tcgcgtcaag
     6961 gtgtcttccg ctcttctttg cccccctgga tgcggcgctc gtccgtgccc gccttgagga
     7021 gatactggcc gcggaggggg cgagcgtccc aaaagacgat ctcgacctga tcgtctgtgc
     7081 cgcacacggc gacctgcggc gggctataac ttacctccag attgcagcag ggtcggggga
     7141 ggaggggttc gaccttgcgg aggttgcgcg gtcggagacc ggcaacatcg ccgcatcggc
     7201 gtttgaggcg ctccgcggcg gcaacctgga ggctgcccgc gggatcgttg agtcgctttt
     7261 gatcgactac gggctctcgg cgcaggaggc tgtcgttgaa ctccgggagg cgatccggcg
     7321 cgactataac cacccggccc ttgttatagc cctcgccgat acggatctgc ggctcacgca
     7381 cagcgcaaac gattttgtgc agctaaatgc attgcttgcc cggatcgcgc tggaggtgtc
     7441 tggtgaagaa ggtacagcag cactatgacg agattgcgga tgtatacgat gagcgctacg
     7501 acggcaggcg ggggaggtgc taccacgggc acatctgcga ccacgtgatg cggaatctcc
     7561 cgaaaggcgg gtttctgctg gacgtcggtt gcggaaccgg actctttgtg cagcgctacg
     7621 tagaggaggg gggccgtgcg gtcgggctcg atgtcagccc ggggatggtc cggcacggcc
     7681 gggggcgctg cccggactgc gggttctgtg tcgggacggc cgatgcgctg ccgtttgagg
     7741 acgggagttt cgatgccctg gcaagcctgc ttgccttcag ttacatcccc gacccggagg
     7801 cgacgctccg ggagtgttac cgcgtcctcg cacccggcgg ccggatagcc gtctgcacgc
     7861 tctcgcggac ggtctttaca agcatcgtcc ccttcgtcta caggatgagc gagaggatag
     7921 gcctgagggg tgtggggatg ggcgatttcg atgagcacta ctacaccaac gcggagatgg
     7981 caagactctt ttgcgatgcc gggtttgagg atgtcacgat cagccggtgc tcgtttgcgc
     8041 acctgaaact catcgaaccg atctttaccc tcgcgcggaa ggttgagccc ttcatcgaga
     8101 agagactgcc gtacctggct tacaacgtct gtgcaagcgg gaaaaaaccg gagaattagg
     8161 ccacatttat gaagggggcg cacccgggtg ttttgccaga cgttaagcgc ccggggcggc
     8221 tttgcgtccg ctcccgcggc aaacaggctc ttcggccggc cggccgccgg gaggagatgg
     8281 cgaaatgccc tggtttttgc gttacgggga cgattaacgg aacggttata tcccgcctcc
     8341 cccgaacaac tcccctatgg agccggtcga tatcgtcctg acgaaggaga cgttcgaggt
     8401 cctggcgtcc ggtaccaggc tcacgatctt gaagaacctg catgaacgca ggatgacgat
     8461 cacggagctc gcggacggcc tcggcctggc aaagtccacc gtccatcacc acctgcagag
     8521 gcttgccgac gccggtcttg tcgctgccga tgacgacggg cgcgcctggg tgtactacgc
     8581 gctcacgccg gaggggcggg ccctcctgca gccgcacggc agcgcccgga tccggattat
     8641 cctgactgcg gggctcgcaa gcctcgccgg tggggttgtt gccgtcgcgg ccttcttgac
     8701 ggcgccgatc cgtgaagtgc cggtgccccc gatcgcggga ggcggcggcc tcccggtccc
     8761 ggagggggct ccgacagaac tgctcgctgc cgggatcgcg ctcgcggtga tcggggtgat
     8821 tctcatagcg tatgcctgcg cccggcggcg gggggcttcc ccgcggacga gggggtcacg
     8881 ggacctctat aggtagtttt cggatccgtt cgaccggatt cgaacggttt cgccggtttt
     8941 atagtcttta ttgtgagata gaatccctgt ggatgccccg ccttttcctc cggtgcgatc
     9001 cacgggagat aacgatgaaa aaactgttga tattctccgg agtatgtgtc ctcgtcctgg
     9061 cgctcattgc tgcaggactg agcgccgccg gcaccacccg tcccgatacg ccggacggcg
     9121 ccggcggtgt ggcggccgga aacaacctgt tcgcgttcga cctctaccgg caccttgccg
     9181 ccgacccggc gtatgcagga gagaacctct tcttctcgcc ctacagcatc gcatcagccc
     9241 tcgcgatcac atgcgagggc gcccgcggca cgaccgccga cgagatcgaa tcggtgctgc
     9301 accttccaac gaacgagacc ctccggcggg aggggtttgc tgacctcaat gccgccctga
     9361 acagcgggag cggcaactgc accctccgca ccgcaaacgc cctctgggca gagaagaccc
     9421 attcgctcct tccggggtat attgagacgg ccgcccgctg gtatggcgca aacgccacaa
     9481 acctcgattt catcgagaac cccgaggggt cgcgggagac gatcaaccgg tgggtggagg
     9541 agaagaccga agatcggatc cgcgacctcc tgccgccggg ttcgatcgac ccgctgaccc
     9601 ggctcgtgat cacgaacgcg atctacttca aaggcgcatg ggcgcagcag ttcgaccccg
     9661 ccaggacgac ggaggaggag ttccgggttt caccgaacga gacggtggcc gtcccgatga
     9721 tgcacgggaa tgccgtctac ccctacgcgg agaccgggac actccaggtg cttgagatgc
     9781 cgtatttcca cgggaacgga acggaactct cgatgctcgt ccttctgccg aaggaagaca
     9841 gcctgacggc tgcggaggag gccctggacg cggacctgcg agagtcgctc gccccgcaga
     9901 acgtcagggt cttcttcccg aagttcacgc tggagacgga gtatcctctt ccgggaaccc
     9961 ttgcggcgat ggggatgccg acggctttca ccggcgatgc cgacctctcg gggatggacg
    10021 ggacgagaga cctcttcgtc accggagtct tccataaggc gttcatcgac gtgaacgagg
    10081 agggcaccga ggccgcggca gcgaccggcg ttgcggccgg gaagggtgtc acacccgtct
    10141 tccgtgcgga ccacccgttc gtcttcctca tcaccgagaa ggattccggc gcgatcctct
    10201 tcatggggag ggtcgtcaac cccgaaagcc cgtgaagggg ggctgt
//
LOCUS       DAOSZX010000048        10225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_5578_length_10225_cov_22.054376, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000048 DAOSZX010000000
VERSION     DAOSZX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5578_length_10225_cov_22.054376"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK196_05275"
     CDS             <1..741
                     /locus_tag="PK196_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division control protein Cdc6"
                     /protein_id="HON40862.1"
                     /translation="TRINSDLRLAKVSIIGISNDLRFTDFLDPRVLSSLSEEEIVFPP
                     YNAPQLCDILQQRAEMAFVEGVLDGAVIPLCAALAAQEHGDARRALDLLRVSGELADR
                     ENADRVTEKHVRMAQEKIETDSMVECISTLPTQSKAVLYAMLILERMGKRIFTSGEVT
                     VVYREIARIIDLDVLTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWFDAAVSKI
                     DEVVTRDPRLDDERLKELDINRLKMMFR"
     gene            747..1238
                     /locus_tag="PK196_05280"
     CDS             747..1238
                     /locus_tag="PK196_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HON40863.1"
                     /translation="MDENDRILLHLLEENCRTPLSELAVLAEMSEQDVKDRISRLEAT
                     GVIRHYGAVVDWERAGDGQVTSVIELKVSPERDFGYDRIAERIARFPQVRSLRLMTGA
                     YDLQLLVTGSSMHEIARFVAEQIAPMDRIRETATHIIMKTYKENGTTFVERDEVERLP
                     YSF"
     gene            1250..2434
                     /locus_tag="PK196_05285"
     CDS             1250..2434
                     /locus_tag="PK196_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON40864.1"
                     /translation="MRSFISERASAIPPSGIRKFFDIAQTMDDVISLGVGEPDFVTPW
                     CICEAAIYSIEQGSTAYTSNKGTPQLRGAISHYLDTRFGTAYDPEEEIIVTCGVSEAA
                     DIAIRAVVDPGDEVLVAEPCYVSYIPCVTLAGGTPVPVPCRAEDEFRLAADALAEKIT
                     PRTKILIANFPNNPTGGVMGESDWRAIADLLVDHDLLLISDEVYAELTYEGGHFSPAR
                     IPEIRDRTITLNGFSKAFAMTGWRIGYLCAPQGITAAALKIHQYVALCAPVMGQIAAY
                     EALRIGEEEKDAMVREYRLRRNLFVDGLNKLGLSCHLPKGAFYAFPSVESTGMSDTEF
                     AEALLREQHVAVVPGSVLGPSGAGHVRCAYAVSRDDLKEALSRMGAFLESHKLSEQVS
                     QN"
     gene            complement(2791..>3190)
                     /locus_tag="PK196_05290"
     CDS             complement(2791..>3190)
                     /locus_tag="PK196_05290"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HON40865.1"
                     /translation="NLVEQREFTISIPSVNYIRETDYFGIVSGRDEDKFAVTGLTPVK
                     GDLVNAPYVGEFPVVLECRLLQTVEIGVHTQFIGEILDVKVDEGVLGEDGRPDLEKIR
                     PFAYDSIRQEYYGHGKPLGKAFSAGKGIKR"
     assembly_gap    3191..3290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3293..4222)
                     /locus_tag="PK196_05295"
     CDS             complement(3293..4222)
                     /locus_tag="PK196_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HON40866.1"
                     /translation="MDWLLIILTIAGLIVFETVSSVDNAIINADVLATMSQRARRWFL
                     VWGLIIAVFVVRGLLPWLIVWMTTPSLGPIEALLATFSSDPAVVAAVEESAPILLIFG
                     GMFLILLFFHWLFAEEKNCYLRSERFFSRQAVWFYAIASILLSVIVWFALQINTMMGF
                     AAVLGSTVFFIVHGFRKNAEVQEARMMGSGMSDVSKLAYLEVLDATFSIDGVIGAFAF
                     TLSVPLILIGNGVGAFVVRELTVRGVDQIRKFCYLKNGAMYSILVLGTIMLADSFGFR
                     IPAWLSPIATFMIVGFFLFRSVQQQKKGAVGSA"
     gene            complement(4458..4925)
                     /locus_tag="PK196_05300"
     CDS             complement(4458..4925)
                     /locus_tag="PK196_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HON40867.1"
                     /translation="MEKLIVKLDFEDFPEEYTSRGANRSPPIRVEGLLPEIRSLAIMV
                     MACPEDGASRVAWVTWNLPAVPQIPEGFPPGGEVDSPLHAVQGENDFGTIGYRGPAPD
                     AGKPEAYLFRVYALDREVEIKAGSTWDEMVMAIRGSIKQVGEAIAFAEEQVAC"
     gene            5249..5716
                     /locus_tag="PK196_05305"
     CDS             5249..5716
                     /locus_tag="PK196_05305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HON40868.1"
                     /translation="MTKKGDRLEDTAMTPGADALIVYTDGASRGNPGDAAWAYVIVRG
                     GSVVAWRSGYLGKTTNNVAEYHAIINALRAARELTSGSVLVRSDSELVVRQVTGRYRI
                     RKEHLAGLAEEVWRLMRDFTEVQFESVPREHPCIKVVDRLCNETLDARPQVKE"
     gene            5724..6161
                     /gene="tsaA"
                     /locus_tag="PK196_05310"
     CDS             5724..6161
                     /gene="tsaA"
                     /locus_tag="PK196_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HON40869.1"
                     /translation="MERIECIPIGIVRSPYRNSGDAPRQGRLADTVAEIHVHDACAPG
                     LEGIERSSHLIILYWLDRAERGQLYAKPPGESRERGVFSTRSPARPNPIGFGVVDLIG
                     REGNVLRVRGLDALDGTPVLDIKPYSPEIDCVPEATGGWRIKK"
     gene            complement(6168..7967)
                     /locus_tag="PK196_05315"
     CDS             complement(6168..7967)
                     /locus_tag="PK196_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HON40870.1"
                     /translation="MNGYAEMILHANLETGEMREEPFPEGWRRAYIGGRGLGVRILQD
                     LVDPGIDPLSPENVLVIATGPLAGSGLPVGSRYGVTAKSPLTGTLSSSNSGGKFGTSL
                     KRAGYDAIVVHGRAERPVYIHIDDGEARIRDASDLWGMTTAEATATLQKEIGDPALSV
                     ACIGPAGERLVRFAAIINETSRAAGRGGLGAVMGSKNLKAVTASGNGRITVADHDRFT
                     ALKREMAEKIRKNPISGEGLPGFGTAVLVNIINENYILPTRNFQTAHFPAAEKVSGER
                     MAESILVGKMGCQGCIIRCGRDVEVEGKRTAGPEYETIWAFGPDCGIDDLAAVVEANN
                     ICNDLGLDTISTGSTIACAMELSERGYIQDEIRFGDAELMLDLVQRIGYREGIGDELA
                     EGSFRFARKHGHPELSMSVKRQELPAYDPRGLQGHGLAYATSVRGGDHVYAYLIAPEV
                     LGSPEKIDPYSSEGKAVWTKTFQDLTAFIDSSGSCLFTSFPLGAADYGAMASAVTGYD
                     IDAAEVLRIGERIWNMQKIFNLKAGCSREDDTLPPRLLREPLTEGAPSGRVWEREPLL
                     DEYYQVRGWDGEGRPTPEKLRELGIGEDGVAIP"
     gene            8141..8446
                     /locus_tag="PK196_05320"
     CDS             8141..8446
                     /locus_tag="PK196_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HON40871.1"
                     /translation="MQTTGEGGTINVRVRTFARLREVLPAEEDLVLPGGSNIGDLLDQ
                     LTARYPGAADLIFAPEGGLRRYINILKNGRNIHFSGGLETPLKDGDTVAIFPPAGGG"
     gene            8575..9477
                     /locus_tag="PK196_05325"
     CDS             8575..9477
                     /locus_tag="PK196_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HON40872.1"
                     /translation="MTQTNEPTNETCAGNCTACPSATKCDDPRNTAEPKGLPPKAEIS
                     VKHVVLVLSGKGGVGKSTVSANLAYALANRGYATGLIDLDIHGPDIPKMLGIEDARLQ
                     SYDGKVIEPVRVTGNLAVVSMAFLLPERSTPVIWRGPMKMAVIRQFLEDVNWGDLDYL
                     IVDLPPGTGDEALTIAQLAPNIAGAVIVTTPQDVAILDSSKAVEFVKKLELPVLGIVE
                     NMSGFVCPHCKAEIDIFGRGGGKREAENLGVPFLGSIPLDLEMRQAADEGRPFIIRRT
                     GAAESPTWKSFDAILQALVDQIGD"
     gene            9479..9790
                     /locus_tag="PK196_05330"
     CDS             9479..9790
                     /locus_tag="PK196_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40873.1"
                     /translation="MDYSRVLAGREAPLPIFAQIVEALEEFEEFPLLIEPIYREASKL
                     EEEDLDRLRFGLLRLQVYADIHRYEDVEVAQRMKYVGTTLERLLFGRLLLEGEVEMQQ
                     C"
BASE COUNT         2079 a         2940 c         3052 g         2054 t
ORIGIN      
        1 acccggatca actctgacct ccgacttgca aaagtcagca tcatcgggat ctcaaacgac
       61 ttgcggttca ctgattttct cgacccccgt gtcctgtcgt ccctctctga agaggagatc
      121 gtcttccccc catacaatgc cccgcaactc tgcgatatcc tccagcagag ggctgagatg
      181 gcattcgttg agggtgtgct cgatggtgcg gtcatccctc tctgcgcggc gcttgctgca
      241 caggagcacg gtgatgcccg gcgggcgctg gacctcctcc gggtctcagg cgagcttgcg
      301 gaccgcgaga atgcagaccg cgtgaccgag aagcatgtca ggatggccca ggagaagatt
      361 gagaccgata gtatggttga gtgtatctcc acacttccga cacagagtaa ggcggttctc
      421 tacgcgatgc tgatcctgga gcggatgggc aaacggatct ttacaagtgg tgaggtcacg
      481 gttgtctacc gtgagatcgc ccggatcata gatctcgatg tccttacgca ccgacggatc
      541 accgatctca tctccgagct caatatgcta ggggtcatca acaccagggt ggtctcacgt
      601 gggcgttacg gccggacgaa ggagatgtgg ttcgatgcag cggtgagcaa gatcgatgag
      661 gttgttacgc gtgacccgag gcttgatgat gagagactca aagaactcga tatcaatcgg
      721 ttgaaaatga tgttcaggtg aaacccatgg acgaaaatga tcgtatcctc cttcacctcc
      781 tggaggagaa ctgtcgcacc ccgttaagtg aacttgcggt gctggctgag atgagcgaac
      841 aggatgttaa agaccggatc agtcggctgg aagccacagg cgtcatccgg cattatgggg
      901 cggtcgttga ctgggaaagg gcaggcgatg ggcaggtcac ctcggtgatc gaactgaagg
      961 tgtcgcccga gcgggacttt ggctacgaca ggattgccga gcggatcgcg cgattccccc
     1021 aggtccgttc gctcaggttg atgaccggtg catacgatct ccagctcctg gtaacaggat
     1081 cgagcatgca tgagatcgcg cggtttgtgg ccgagcagat cgccccgatg gacaggatcc
     1141 gggagactgc gacgcacatc attatgaaga cctacaagga gaacggcaca accttcgtcg
     1201 aacgcgacga ggtcgagcgc ctgccctact ctttctgagg tgatgagcgt tgcggagttt
     1261 catctcggag cgggccagcg ccataccgcc gtcgggtatc cgtaagttct ttgacatcgc
     1321 tcagacgatg gatgatgtca tatctctggg tgtaggtgaa ccggactttg tgacgccctg
     1381 gtgtatctgc gaggctgcca tctactctat cgagcagggg tcgaccgcct acacctcgaa
     1441 caaggggacg ccacaactca ggggtgccat cagccactac ctggatacca gattcggtac
     1501 cgcctacgat ccggaggaag agatcatagt gacctgcggc gtctcggagg cggctgatat
     1561 tgccatccgg gctgtcgttg atcccggcga tgaggtgctt gtcgccgaac cctgttacgt
     1621 ctcctatatc ccctgtgtca cgcttgccgg cgggacgccg gtgcctgttc cgtgccgggc
     1681 agaggatgag ttccggctgg cggccgatgc gcttgcagag aagatcacgc cacggacgaa
     1741 gatcctgatc gcaaacttcc ctaacaaccc gaccggtggg gtgatggggg aatcggactg
     1801 gcgggccatc gccgatctcc tggttgacca tgacctgctc ctgatcagcg atgaggtcta
     1861 cgccgagtta acctatgaag gcggccactt ctcacctgcg aggatcccgg agatccgcga
     1921 ccggacgatc accctgaatg gtttctcaaa ggcttttgcc atgacagggt ggaggatcgg
     1981 ctacctctgc gccccgcagg ggatcacagc ggcggcgttg aagatccacc agtatgtcgc
     2041 gctctgcgct cctgtcatgg ggcagatcgc ggcctatgag gcgctcagga tcggggagga
     2101 ggagaaggac gcaatggtca gggagtatcg tctcaggcgc aacctctttg ttgacgggct
     2161 gaacaaactc ggtctctcct gccacctccc gaagggtgca ttctatgcct tcccctcggt
     2221 ggagagcact ggtatgagcg atacggagtt tgcagaggcg cttctgcgtg agcagcatgt
     2281 ggctgtagtt cccggaagcg ttctcggccc ttccggtgcc gggcacgtgc ggtgcgccta
     2341 cgcggtctca cgcgatgacc tcaaggaggc tctttccagg atgggagcct tcctcgaatc
     2401 acataaatta tcggaacagg tctcccaaaa ttaaaatcga aaccaattta agagagatcc
     2461 ctgtacctgc aatcgtctat ctgctgtccg acggaggaat gactcccgga gaaccccttt
     2521 atttccactg gagatgtgaa tttattccta gatcccggcc ggtggggatc gcgctgcggt
     2581 cttgcccgaa gtatgccttc aggttggaag accgcggata gaactccgat ctttcatctg
     2641 tctaccaggc gggtgatctt gctgtaattc agtctcctct tcggagatgg agatccctgt
     2701 atgcgctcgg cgccgggcgc agggaggaga tctccagtgg aaacgaaagc gtcatggcgg
     2761 cgcacgcctc ctgcggtggg aggaaaaaag ttatcgtttg atccccttcc cggcagagaa
     2821 ggcttttcca agtggtttcc catgcccata gtactcctgc cggatggagt cgtaggcaaa
     2881 tggtcggatc ttctcgagat cgggtctgcc atcctccccc aagaccccct cgtccacttt
     2941 cacgtccagg atctcgccga tgaactgggt gtgcacgccg atctcgaccg tctggagaag
     3001 gcggcattcg aggacgaccg ggaactcccc cacgtagggg gcgttcacca ggtcaccttt
     3061 aaccggtgtg aggcctgtaa cggcaaactt gtcctcgtcc cggccggaga cgataccgaa
     3121 gtagtcggtc tccctgatgt agttgacgga tgggatactg atcgtgaact cgcgctgctc
     3181 gaccaggttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctcacgcgc
     3301 tacccaccgc tcctttcttc tgttgctgca ccgagcggaa gaggaaaaac cctacgatca
     3361 taaacgttgc tataggcgag agccaggcgg ggatacggaa cccgaaactg tccgcgagca
     3421 tgatcgtccc cagcaccagg atagaataca tcgccccgtt tttgaggtaa cagaatttcc
     3481 tgatctggtc gacgcccctg acggtaagtt cccttactac gaatgccccg acaccgtttc
     3541 ctatcaggat aagagggaca gagagggtga aagcaaacgc cccgatgact ccatcgatcg
     3601 agaacgttgc atcaagtacc tcgaggtagg caagtttaga aacatcagac atgcctgacc
     3661 ccatcatacg ggcctcctgc acctcggcgt tctttcggaa accgtggacg atgaagaaga
     3721 cggtggaacc gagcacggct gcgaagccca tcatggtgtt gatctggagg gcaaaccaga
     3781 ctataacaga aagcaggatc gaggcgatgg cgtagaacca gacggcctgc ctggagaaga
     3841 aacgctcgct cctgaggtag cagttcttct cctccgcaaa gagccagtga aagaagagga
     3901 gaatgaggaa catcccacca aatatcagga ggatcggggc ggactcttcg accgcggcca
     3961 ctacggcggg atcgctcgag aaggttgcga gcagcgcttc aatgggccca agcgagggtg
     4021 tcgtcatcca gacaatcaac cacggcaaca acccgcggac aacaaagaca gcaatgatca
     4081 gaccccagac aagaaaccac ctgcgtgccc tttggctcat cgttgcgagg acgtcggcgt
     4141 ttatgattgc gttatctacg cttgagaccg tctcgaagac gatgaggcct gcaatggtaa
     4201 ggattatcag cagccagtcc attatgcggc tgtagagtct gctgctgatt gtatattaaa
     4261 gatgggataa gacgcgtata cggctaatgt cgaagcagag ttgcctgtgg gaggcggtta
     4321 ggggcgaagg ccatggtcct ggagaacagt ggatctgcgg ggccgttcag ttgatgggcg
     4381 gtgcggcgac tcaaaggagg gacccgcgcg agaggctggc cgccatcccg cttgaagatc
     4441 attttcctgt atccccctca gcaagccacc tgctcttcgg caaaggctat ggcctcgccg
     4501 acctgtttta tcgacccgcg aattgccatg accatctcat cccaggtcga gcctgctttg
     4561 atctcaacct cacggtcaag cgcatacacc cggaataggt aggcctccgg tttaccggcg
     4621 tctggcgcgg gcccccggta accgatagtg ccaaaatcgt tctcgccctg aacagcatga
     4681 agcggtgagt ccacctcacc gccgggaggg aacccttccg ggatctgcgg gaccgcaggg
     4741 aggttccagg tgacccatgc cacccgtgag gccccatcct ccgggcatgc catcaccatg
     4801 atcgcaagcg acctgatctc cgggaggagc ccctcaaccc ggatcggcgg tgaacggttt
     4861 gcccctctgc tggtgtattc ctcgggaaaa tcctcaaaat ccagtttcac aatcagtttc
     4921 tccataatat ccggctccgt tgatactctt tctggaagca ggagataaaa aaggttcgta
     4981 acacttcatc ttgagcagtg gcggcggtct cgccgaatcc ggggacgtgc ccgcgcattg
     5041 agttgatggt gtcctgcttc agagcgcctg caggcagatt cgagcatccc gtcaacctta
     5101 gacctttggg aggtagcgat gaatctcgcc acgcagcagg caggggccgc gcaggccgat
     5161 ggtcccataa tcgtttgata acctcatgtc ttctggagcg gtggagacga taccaataac
     5221 ttccgggaga tcgatgatca accggaagat gacgaaaaag ggtgacagac tggaggatac
     5281 ggccatgacc ccgggggcgg atgcgctgat agtctacacc gacggcgctt ccagggggaa
     5341 ccccggagat gccgcgtggg cttacgtgat cgtgcgtggc ggttccgtcg tggcctggcg
     5401 ttcgggctac ctgggtaaga cgaccaacaa cgttgccgag taccatgcca tcatcaacgc
     5461 tcttcgcgct gcccgtgagt taaccagcgg gagcgtcctg gtaagatcgg actcggaact
     5521 ggtggtgcgc caggtcaccg gacgctaccg catcagaaag gagcaccttg ccggtcttgc
     5581 agaagaggtg tggcggctga tgcgggattt caccgaggtc cagtttgaga gcgtcccgcg
     5641 tgaacatccc tgcatcaagg tcgtggaccg tctctgcaac gagactctcg atgcaaggcc
     5701 gcaggtaaag gagtgagcgc ggcatggaaa ggatcgagtg catcccgata gggatcgtcc
     5761 gctctcccta caggaattcg ggcgatgccc cccggcaggg acggctggcg gataccgtcg
     5821 cggagatcca cgtccatgac gcctgcgccc ccggtcttga aggcatcgag cgcagcagcc
     5881 atctcataat cctctactgg ctcgaccgcg cggagcgtgg gcaactctac gcaaagcccc
     5941 ctggagagtc ccgggaacgg ggggtcttct ccacccgctc gccagccagg ccaaacccta
     6001 tcggtttcgg ggttgtcgac ctgatcggga gggaaggaaa cgtcctccgg gtcagggggc
     6061 tcgacgccct ggatgggacg ccggtgctcg atatcaagcc ttactccccc gagattgact
     6121 gtgtcccgga ggcaacgggc gggtggcgca tcaaaaagta ggcccggtca ggggatggcc
     6181 accccgtcct cccctatgcc gagttcgcgg agtttctcgg gcgtgggacg cccctccccg
     6241 tcccatcccc tgacctggta atactcatcc aggagtggct cgcgttccca gacccggccg
     6301 ctcggagcgc cttcggtgag cggctccctg agaagccttg gcggtagcgt atcgtcctcc
     6361 cggctgcacc ctgctttcag gttgaagatc ttttgcatgt tccagatccg ttcgcctatc
     6421 cggagcacct ccgcggcatc gatatcgtaa ccggtgaccg cagaggccat cgctccataa
     6481 tcagccgcac cgagcgggaa agaggtgaag aggcagctac ccgaagagtc gatgaaagcc
     6541 gtgaggtcct ggaacgtctt tgtccagacc gccttcccct cacttgagta ggggtcgatc
     6601 ttctccggcg atcccagcac ctcgggtgcg atcaggtagg catagacgtg gtcaccgccc
     6661 ctgaccgagg tcgcgtaggc aaggccgtgc ccctgcagcc cgcgggggtc gtaggcgggg
     6721 agttcctgcc gcttgacgct catggagagt tcggggtgcc catgttttct cgcgaaccgg
     6781 aacgagcctt cggcaagttc gtcgccgata ccctcccgat aacctatcct ctggacaagg
     6841 tcgagcatca gttccgcgtc gccgaaccgg atctcatcct ggatgtagcc cctctccgag
     6901 agttccatgg cgcatgcgat cgtcgatccg gtagagatcg tgtcaagccc gagatcgttg
     6961 cagatgttgt ttgcctccac aacggcagcc aggtcgtcga tcccgcagtc cgggccgaac
     7021 gcccagattg tctcgtactc gggcccggcc gtccgcttcc cctcaacctc gacgtcgcgg
     7081 ccgcaccgga tgatacagcc ctggcacccc atcttcccga caaggatgct ctccgccatc
     7141 cgttcgccag agaccttctc cgcggcaggg aaatgcgctg tctggaagtt cctggtcggc
     7201 aggatgtagt tctcgttgat gatgttgaca aggacagcgg tcccaaaacc gggcagcccc
     7261 tctcccgaga tggggttctt gcggatcttc tccgccatct ctctcttcag ggcggtgaaa
     7321 cggtcgtggt cggcgacggt gatccggccg tttccgcttg cggtgacggc cttcaggttc
     7381 ttcgacccca tcaccgcgcc gaggcccccg cgcccggcgg cccgcgatgt ctcgttgatg
     7441 atggcggcaa accggacgag gcgttcaccg gcggggccta tacaggccac gctgagagcg
     7501 ggatcgccaa tctctttctg gagagttgcg gtggcctcgg cggttgtcat cccccacagg
     7561 tcagaggcgt cccggatccg ggcttctcca tcatcgatgt ggatgtagac cggtctctcc
     7621 gcccggccat ggacaacgat tgcatcgtag ccggcgcgtt tcaggcttgt gccgaacttt
     7681 ccaccggagt tcgagctgct cagcgtccct gtcagtggag attttgctgt gacaccgtag
     7741 cgcgacccca ccggaaggcc gctcccggca agcggtccgg tggcgatcac cagcacgttc
     7801 tccgggctga gcgggtcgat gccagggtcg accagatcct ggaggatccg gacgccgagc
     7861 cccctccctc cgatgtatgc ccgtctccat ccctctggaa acggctcctc tctcatctcc
     7921 ccggtctcca ggttggcgtg gaggatcatc tctgcgtatc cgttcatggg ttttcacaaa
     7981 ccctgatgac tctttactgg gttataaggc tgctggacgt ggaaaaatgt taactcttgc
     8041 atgaaagaaa ggagatccct cgcaccgggc tcgcgggagc gataccgata agaccaatgg
     8101 atcttctacc accattgtaa agcaggggag tggatctggt ttgcagacta cgggagaggg
     8161 aggcaccata aacgtgaggg tcaggacgtt tgcaaggctc agggaggtcc tcccggcgga
     8221 ggaggatctc gtcctgccgg gaggatcgaa tatcggcgac ctgctcgatc aactcaccgc
     8281 caggtatccc ggggccgcgg atctcatatt cgccccggaa ggcgggctca ggcggtacat
     8341 caacatcctg aagaacggca ggaacatcca tttcagtggg ggtctcgaga caccgcttaa
     8401 ggacggcgat acggttgcca tcttcccgcc cgccggtggc gggtaggcct tatttgaagc
     8461 accaccctgg cggcagaact ggaaaaattg ccagcaccta tacctttttg ttgcatttga
     8521 cgctaacagt ataaggtact tttagaccct tttcacgaag agaggatacc tggcatgact
     8581 cagacaaacg aacccactaa cgagacctgc gcgggaaact gcaccgcctg cccgtccgcg
     8641 acgaagtgcg acgacccgag gaacactgcc gaaccaaagg gtcttccccc gaaagccgag
     8701 atcagcgtca agcacgtcgt cctggtcctc tccgggaagg gcggtgtcgg gaagagcacg
     8761 gtttcggcaa acctcgccta cgcacttgcc aaccgcggct atgccacagg gcttatcgac
     8821 cttgatatcc acggtccgga catcccgaag atgctcggga tcgaagatgc cagacttcag
     8881 tcttacgacg gtaaggttat cgagcccgtc agggtcaccg gaaacctggc agttgtttcg
     8941 atggcattcc tgcttcctga gcgcagcacc cctgtgatct ggcgcggtcc catgaagatg
     9001 gcggtgatcc gccagttcct ggaggacgtc aactggggcg accttgacta cctgatcgtc
     9061 gacctcccgc cgggaaccgg tgatgaggcg ctcaccatcg cccagctcgc cccgaacata
     9121 gccggcgcgg tcatcgtcac cacgccgcag gacgtcgcga tccttgactc gagcaaggcg
     9181 gtcgagtttg ttaagaaact cgaacttccg gtgctcggga tagtcgagaa catgagcgga
     9241 tttgtctgcc cccactgcaa ggcggagata gatatctttg gcaggggcgg cgggaagaga
     9301 gaggcggaga acctgggtgt gcctttcctc gggtccatcc cgctggatct ggaaatgcgg
     9361 caggcggcag atgaggggag accgtttatc atccgcagga ccggcgcggc agagagcccg
     9421 acctggaaga gttttgatgc catcctgcag gccctggtcg accagatcgg cgattaagat
     9481 ggactactcg agggttctcg ctggacggga ggctcctctc ccgatctttg cgcagattgt
     9541 tgaggcgctc gaggagtttg aggagtttcc cttacttatc gagccgatct accgcgaggc
     9601 ctcaaaactc gaggaggaag atctcgaccg tctccggttc ggtcttctcc gcctccaggt
     9661 atacgctgat atccaccgct acgaagatgt ggaggtggcg cagcggatga agtatgtggg
     9721 cacaactctt gagcggcttc tctttggcag gcttctcctg gagggagagg ttgagatgca
     9781 gcagtgctga cgacacgaga ctcccggcac ttgaagtaac cccagacatc ctttatcagc
     9841 cggggcaatc aaaccagtaa cttctggctg tgccctggtt tgatggccac cgcgggcggt
     9901 ttcggtggcg agaaaggatt ttcatcgtgg gttcccggct ggatccgcat agatctcatc
     9961 tgcgcccccc ggcaaaggtt tgatggggcg ggaggaggcc ttgtttagcg gccgggcggc
    10021 gggggtggtc gacgaaagat gtttagcaga gggcgtgtag agacagggag tggaaacgtc
    10081 ccgttccatt tcccgtcaca gccccccgga tggcgattcc cctttgatac actgccatgg
    10141 cgcgggtttt cgcatgaaaa tccaatcgag atcggaaacg gtgtccgcag gctgttgcgt
    10201 tccagacgcg acataccagc ggcga
//
LOCUS       DAOSZX010000049        10111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_5657_length_10111_cov_18.485680, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000049 DAOSZX010000000
VERSION     DAOSZX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_10111_cov_18.485680"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1090
                     /locus_tag="PK196_05335"
     CDS             80..1090
                     /locus_tag="PK196_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HON40874.1"
                     /translation="MPQESGAAVEGRYLSPGCVLCHQGAKMVLFVTGRCNRGCWYCPL
                     SRERKGRDVIFANEHQVTSPAGAIEVAESMSALGTGVTGGEPLLEIERVVDYCRALKE
                     HFGPGHQIHLYTGLAPDEAVLRRLRGLVDEIRLHPPREVWPCILETDYARSAVIARNM
                     GFLVGIEVPSLPGIDDLAAALPLLDFLNINELEWGETCAAAMRKRGFEPDDGVHNAVK
                     GARAWAEKISADPKVHFCSSVFKDSVQLRERLKRIASNTARPFDEVTDDGTVVYGVLE
                     PDGAVDAFLASLDEDDYTVSGGRIEMAWWVLIEHGADLPGKKYVVERYPNNGMIVEVT
                     PL"
     gene            1087..1863
                     /gene="uppS"
                     /locus_tag="PK196_05340"
     CDS             1087..1863
                     /gene="uppS"
                     /locus_tag="PK196_05340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HON40875.1"
                     /translation="MRLRSGIEPLYERYLRRQVRHVPRHIAVIQDGNRRYAQKQGVDT
                     SIGHRMGADTTERVLDWAQELGVQQITLYAFSTENFRRDESELEALFTLFKEKFRAIL
                     DDERVKKKRIRVRMIGDRSLLPADLLEAIDAAEEATRQYSNYFINVAIAYGGRNEIVH
                     AARSILEKVRQGAIDPDSIDPATVERHLSRGALIPPVDLIIRTGNDYRTSNFLPWLAN
                     GHESAVYFCAPYWPAFRKIDLLRAMRVYDQRMRLKERTAG"
     assembly_gap    1865..1964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1965..2518)
                     /locus_tag="PK196_05345"
     CDS             complement(<1965..2518)
                     /locus_tag="PK196_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HON40876.1"
                     /translation="MIYRLYEKILLRNLSTLPEHICFMISEKDMLDAPDNLYRTVEWC
                     RELGIRSVTFHISTTDPARLERCLPQIRRVSSIAHLRLHYREGTEVAGEGMDVAVAIG
                     KSGREEIAACIRQMAEEGVEPDSVDEELLESYLTFRYEPDLVIKTGGDHLTDFLIWQS
                     VYSELFFLDVNWALLRKVDFLRALR"
     gene            complement(2515..2655)
                     /locus_tag="PK196_05350"
     CDS             complement(2515..2655)
                     /locus_tag="PK196_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40877.1"
                     /translation="MKLAAETDAHGSEEFFKVRCGVSGARGWWCARADVYSTPASWGW
                     AS"
     gene            2822..3439
                     /locus_tag="PK196_05355"
     CDS             2822..3439
                     /locus_tag="PK196_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HON40878.1"
                     /translation="MVEDYLEAILEIAENSGRPPTVEEIAAALKTGKETASSTIAALA
                     REGYLEQVGDAVRLTEKGAALASKVARKHTVLQCFLTEMLGIDTNSASREACTLEHGI
                     SDDTIERLSSYMSGTATMPGRMRRRRGQETVTLLDCNEGDIARVALMRGPGGHRRLLD
                     LGVFPGEIVEIRRKLPNNSVVVRVKGCDIAISPEIARSIFVESCP"
     gene            3436..5286
                     /gene="feoB"
                     /locus_tag="PK196_05360"
     CDS             3436..5286
                     /gene="feoB"
                     /locus_tag="PK196_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HON40879.1"
                     /translation="MKIVLIGNPNVGKSLIFNQLTGLGVEVSNYPGTTVELQRGNTCF
                     QREIIEIVDLPGVYSLEGNSEEEDLARRFLEQEDVDAVVVVASATRLERNLYLLLQVA
                     EYGLPMVVVLNMVDEAAKRGLEIDPGPLHDLLGVEVLLTAASQGLNIDRIIPAALTAS
                     RPAKIEIPYDHHIEAAIRSLGKIFGVDRKESLRALQGFGDSPELLEAARTIADEIESR
                     HRMTVAQIIAANRHNFARSLAELTVKEKHPPAGTDPDSILTRFIPGMPILVAILVGML
                     LFVFVVGSFLEEAIVEVFEVYAIQPFLALGLHPLLEEVGASILLALQAGLGIALPYVF
                     LFYIIISILEDSGYMTRAAFLADNAMHRLGMHGGAVIPITLAFGCNVPAIMSTRLLHS
                     RRERIIASILVTMVPCSARTVIIAGIVAGFVGLAAAFSVYAIVFVLIILTGLLLSRVV
                     PGERFGMIMEMAPLRRPVPRLVMKKAWSRLSEFLFIAMPLLLLGSVVLGLLEFFGVMT
                     VFEGIVEPYTMGLLGLPGYSATALIFGILRKEMAFETLAILAGTADLGAVLSSLQVYV
                     FAVVTVLFVPCLATITVLLREVGSRITFAITVYTVALGLLIGGFIHHLFS"
     gene            5298..6017
                     /locus_tag="PK196_05365"
     CDS             5298..6017
                     /locus_tag="PK196_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40880.1"
                     /translation="MYIYETGIPLIDGDYGGMHAATNLLLLAPPLSHAEHLAYRIASP
                     RPGEWTVALSTDERAADVVGAFRKLGAERNRLGVIDAVTKGSVPTVRDTPKVKFVTSP
                     LDLTSMGIKFSRMVEDMWKEGAMADPEGPMPPPVRFCLNSVSTILMYAQLEVTFRFLH
                     VITNRVKKLEGVGVYTLNSESFDRRTVSTIKQLMSMVIEVRTDEGRVAADHDFRVIGI
                     HGRTTPWIRYFYSDGVVTIEE"
     gene            6055..6807
                     /gene="dph5"
                     /locus_tag="PK196_05370"
     CDS             6055..6807
                     /gene="dph5"
                     /locus_tag="PK196_05370"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HON40881.1"
                     /translation="MLTFVGLGLYDLGDITLKGLTYVKSADVVFLEAYTSRLMGTDLA
                     AMEVFFGKEIRVLSRDDVEQDPAEILERAAAGRVAFLIGGDPMVSTTHADLRIRAAAA
                     GIETSIIHAASISSAVCGLSGLQNYRFGKSCSVPFPARGWFPTTPIDTITVNLGLNLH
                     TLVYLDIQDGRCMRIPEAIEIIEEMAERRGVEPPALYVGIARAGSEHPVVAAGSGSKL
                     KETDFGPPLHVLAVPADLHPVEREYLETFAGL"
     gene            6804..7373
                     /locus_tag="PK196_05375"
     CDS             6804..7373
                     /locus_tag="PK196_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HON40882.1"
                     /translation="MTLEEYGGLFEGALLRVRIAVPEERPLFRVAAEVMEMAAAYLSD
                     GAAFLEAGDRVNALAAFAYGLGWLDAGSSLGLIEPYDPHPPEAVDARILPSQTARLDE
                     KTRRYRRMLDSALEGVEEAPDVSSPLHAAARGFLSVAQRWHEEGGERLDAGDFNGALL
                     RLSYGYAWLDAGIRAGLFRVTGRRDLFTV"
     gene            7676..8716
                     /locus_tag="PK196_05380"
     CDS             7676..8716
                     /locus_tag="PK196_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HON40883.1"
                     /translation="MNRSQVKWLLVSIGFSVLVLGVVLYFTVDDTTVDYIRRINPFYL
                     LAAVVLHLLSLVFWSLRIQKMSGSLGFRVRFTHCMNLVLANMLVAAVTPSQAGGEPVR
                     IHELYRAGVPVGDATAVVIMERVLDGIVLGALGVFSVLFLGKYWRSLTTGFSGMGVLI
                     GVACAVTTLIVIFFVYSVRNPDCLKRALRWGSRWVDRRWHLKRLEHLLEAIDREVDNF
                     NQALTRFIGHGKGGLFWGLVFSLLFWLSEFVIASFLLMGLGQPPHFIESFIVQILVAI
                     IMMIPLTPGSSGIAELSATSFYSLFVPSSIVGIFVLLWRLILYYLNIILGLLPGLVIV
                     RREVIARARKHR"
     gene            8763..9209
                     /locus_tag="PK196_05385"
     CDS             8763..9209
                     /locus_tag="PK196_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HON40884.1"
                     /translation="MTAQECRVQGVFMAVSDMGAAPTVLLDTGGESMVPIYIGLWEAI
                     SISNALNSEVLPRPITHDLIIEVFRNFEIALDALHIDALEGGVFYAKLMLRQGSRTEM
                     MDCRPSDGIAIALRYPAPIMIEDTVIEAAAVKKADLPQLVDLKEYL"
     gene            complement(9221..9514)
                     /gene="hisE"
                     /locus_tag="PK196_05390"
     CDS             complement(9221..9514)
                     /gene="hisE"
                     /locus_tag="PK196_05390"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HON40885.1"
                     /translation="MRDWAILEELWEVIQDRADHPSTGSYVSSILTHRKGVDKSLEKV
                     GEEAVEFILAAKNQVPERTVSEAADLLFHLLVAFQALGIDPADVLDELAARRK"
     gene            complement(9507..9947)
                     /locus_tag="PK196_05395"
     CDS             complement(9507..9947)
                     /locus_tag="PK196_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HON40886.1"
                     /translation="MVRTISFKERRYIEELRILTRATALDCIIDERFDRIVYLIKEGD
                     MGLAIGRKGSNIRKMQRVLGKRVEMVEYSPELERFTKNVFKPAEVTGVERRDDGKITV
                     YVSKGDLGIAIGKGGCTIEKARNLLSRYFDAELGEVLAGEAAHA"
BASE COUNT         1880 a         2994 c         3035 g         2102 t
ORIGIN      
        1 gggcggagag gatcccggga tatccgtcta ccaaccgtca aacctttttc atcttgtgtg
       61 tcttataact attatcatca tgccgcagga gtccggagca gcagtggagg ggcgctacct
      121 gagcccgggg tgtgtcctct gccaccaggg ggcaaagatg gtcctctttg ttaccgggag
      181 gtgcaaccgc ggctgctggt actgcccgct atcgcgggag cgcaaagggc gggacgtcat
      241 ctttgcaaac gagcaccagg tcacctcccc tgccggtgcc atcgaggtcg cggagagcat
      301 gagcgctctt ggaaccggtg tcaccggcgg cgagcctctg ctcgagatcg agcgggttgt
      361 ggactactgc cgggcactca aagagcattt cgggcccggg caccagatcc atctctacac
      421 cggtcttgca ccggatgagg ccgtgctccg gcggctgcgg gggctggtcg acgagatccg
      481 gctgcacccc ccgcgggagg tctggccctg catcctggag accgattacg cccgttcagc
      541 agttatcgcc aggaacatgg ggttccttgt cgggatcgag gtcccatcgc tccccgggat
      601 cgatgacctt gccgccgccc tcccgctcct tgacttcctc aacatcaacg aactggagtg
      661 gggcgagacc tgtgccgctg ccatgcgcaa acgcggcttt gaacccgatg acggagtgca
      721 caacgccgtg aagggtgccc gggcctgggc ggagaagatc tctgccgacc cgaaagttca
      781 tttctgttca tcggtcttca aggactccgt ccaactgcgg gaacggctta aacggattgc
      841 ttccaacacc gcgcgtccgt ttgatgaggt gacggatgac ggaacggtcg tatacggcgt
      901 gctcgaaccc gatggagccg tcgacgcgtt ccttgcctcc ctcgacgagg atgattatac
      961 agtctccggg gggaggatcg agatggcctg gtgggtgctc atcgagcacg gcgccgacct
     1021 ccccgggaag aagtatgttg tcgaacgcta cccaaacaat gggatgatcg tcgaggtgac
     1081 gccgctctga gactccgatc ggggatcgaa cccctctatg aacgttacct gaggcggcag
     1141 gtgaggcacg tcccccgcca catcgcggtg atccaggacg gaaaccgccg gtacgcgcag
     1201 aaacagggag tggatacgtc gatcggccac cgcatggggg cggacaccac ggaacgggtg
     1261 ctcgactggg cgcaggaact tggcgtgcag cagataacgc tctacgcctt ctccaccgag
     1321 aacttccggc gggacgagag cgaacttgag gcgctcttta cactctttaa agagaagttc
     1381 agggcgatcc tggacgacga gcgggttaag aagaaacgca tccgggtgcg gatgatcggg
     1441 gatcgctcgc tcctgcccgc agacctcctt gaggccatcg atgcagcaga ggaggcgacc
     1501 aggcaataca gcaactactt catcaacgtc gccatagcct acggcggcag gaacgagatc
     1561 gtgcacgccg cccgatcgat cctggaaaag gtcaggcagg gtgccatcga cccggactcc
     1621 attgaccccg caaccgtcga gcgccacctc agccgcggcg cgctcatacc gcccgttgac
     1681 ctgatcatcc gcaccggcaa cgactaccgg acgtccaact tcctcccctg gcttgcaaac
     1741 ggccatgagt cggcggtcta cttctgcgct ccatactggc cggcgtttag aaagatcgac
     1801 cttttgcgcg cgatgcgggt ctatgaccag cgtatgaggc tcaaggagcg cacggccggg
     1861 tgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggagc gcgcgaagga
     1981 aatccacctt ccggagcagc gcccagttga cgtccaggaa gaagagttcg gagtagaccg
     2041 actgccagat caggaaatcg gtcaggtggt ctccccccgt cttgatgacc aggtccggct
     2101 cgtacctgaa ggtgagatag gactcaagca gttcttcatc gacagagtct ggctccaccc
     2161 cctcttcagc catctgccgg atacaggccg cgatctcctc ccgtccgctc ttcccgatcg
     2221 ccaccgccac gtccatgccc tcccccgcaa cctcagttcc ctcccggtag tgcagcctca
     2281 ggtgtgcgat cgaggagacc ctgcggatct gcggcaggca gcgttccagc ctggccgggt
     2341 cggtggtgct gatatggaac gtcacgctcc tgatccccag ttcccggcac cactcaacgg
     2401 tacggtagag gttgtcgggg gcatcgagca tatccttctc gctgatcatg aaacagatat
     2461 gctccgggag ggtgctgagg ttgcgaagga ggatcttctc gtagagccgg tagatcatga
     2521 tgcccacccc caggatgcgg gtgtgctgta aacgtcagcc ctggcgcacc accagccgcg
     2581 cgcccctgaa acaccgcagc gcaccttaaa aaactcctcg ctcccatgag catcggtctc
     2641 tgcagccagt ttcactcctt tcttcttcgc gcgccagata taaatagcct cgagatctgt
     2701 ggtgtgccgg tcaaagtgga tgcaatcaca ggttctggcg agaatacacc caggagcccg
     2761 ttgacaccac ccaaatccat aaactccttg aaacgaaaga gatgttaaca tgctcccttc
     2821 catggttgag gattacctcg aagcgatcct ggagatcgcg gagaacagcg gccggccgcc
     2881 gacggtggaa gagattgcgg cagccctaaa gaccgggaag gagaccgcct catcgaccat
     2941 agccgctctg gccagagaag gctacctgga gcaggtgggc gatgccgtcc ggctgacaga
     3001 gaaaggtgcg gcgctggcct cgaaggttgc ccggaaacac accgtcctcc agtgtttcct
     3061 gacagagatg ctcgggatcg atacaaactc cgcaagcagg gaagcctgca cccttgagca
     3121 cggtatatcc gatgatacaa tcgagcggct ctcctcctac atgagcggca ccgcaaccat
     3181 gccaggacgg atgaggaggc ggcgcggtca ggagaccgtc acgctgctcg actgcaacga
     3241 aggcgatatc gccagggttg cgcttatgcg cggccccggc gggcaccgga gactgcttga
     3301 cctcggggtc ttccccggcg agattgtgga gatccgccga aaactcccga ataattctgt
     3361 tgttgtcagg gtgaaaggct gtgacatcgc catcagcccc gagatagcgc gatcgatatt
     3421 cgtggagtcc tgtccatgaa gatcgttctg atcggcaacc ccaatgtggg taaatccctg
     3481 atcttcaacc agctcacagg tctcggggtt gaggtgagca actacccggg aaccaccgtc
     3541 gaacttcagc gggggaacac ctgttttcag cgcgagataa tcgagatcgt ggacctcccc
     3601 ggcgtctact cgctggaagg gaactcggaa gaggaagacc ttgcccgccg gttcctggag
     3661 caggaggatg tcgacgccgt cgtcgtggtc gcgagcgcca cccgcctgga gcgaaacctc
     3721 tacctcctcc tccaggtggc ggagtacggc ctccccatgg tcgtcgtcct gaacatggtc
     3781 gacgaagccg caaaacgggg gctcgagatc gatcccggcc cgctccacga cctcctgggt
     3841 gtcgaggtcc tcctgacggc cgcatcacag gggctgaata tcgaccggat catcccggcg
     3901 gcccttactg catcccggcc ggcaaagatc gagatcccat acgaccacca catcgaggcg
     3961 gcaatccgga gcctcggcaa gatcttcggg gttgaccgaa aagagagtct ccgggcgctc
     4021 caggggttcg gcgacagccc cgaactgctc gaggcagcaa gaacgatcgc cgacgagata
     4081 gagtcgcgcc accgaatgac ggttgcccag atcatagccg caaaccggca caacttcgcc
     4141 cgcagtctgg ccgaactcac cgtgaaggag aagcacccgc ccgccgggac cgaccctgac
     4201 agcattctga cccgtttcat ccccgggatg ccgatcctgg ttgcaatcct ggtgggaatg
     4261 ctgctctttg tcttcgtggt ggggtcgttc ctggaggagg cgatcgtgga ggtctttgag
     4321 gtctacgcga ttcagccgtt cctcgcgctc gggctgcacc cccttcttga agaggtcggc
     4381 gcctctatcc tgcttgcgct gcaggcaggc ctcggtatag cgctgcctta cgtcttcctc
     4441 ttctacatca tcatatcaat actggaagac tctggctaca tgacacgggc ggcgttcctg
     4501 gccgacaacg ccatgcacag gctcgggatg cacggcgggg cggtcatccc catcaccctg
     4561 gcgttcggct gcaacgttcc ggcaataatg agcacccggc tcctgcactc ccggcgcgag
     4621 aggatcatcg cctcgattct ggtaacgatg gtcccctgct ctgcaaggac ggtcataatc
     4681 gccgggattg ttgcaggttt tgtgggtctt gcggcggcgt tcagcgtcta tgctattgtt
     4741 tttgtcctga tcattcttac cgggcttctt ctttcgcggg ttgtgcccgg tgagcggttt
     4801 ggcatgatca tggagatggc cccgcttcgc cggcctgtgc cgcgactggt gatgaagaag
     4861 gcctggtcac gcttatcgga gttcctcttc atcgcgatgc ccctcctgct tctcgggagc
     4921 gtcgtcctcg ggctgctcga gttcttcggc gtcatgactg tctttgaggg gatcgtggag
     4981 ccgtacacga tggggctcct agggcttccc ggctactcgg cgacggcgct catattcggg
     5041 atcctcagaa aagagatggc gtttgagacc cttgcaatcc tggcgggcac cgcagacctc
     5101 ggggcggttc tatcatcgct ccaggtttat gtctttgcgg ttgtgaccgt cctcttcgtc
     5161 ccctgccttg caacgatcac ggttctcctg cgggaggtcg gttcgcggat cacgttcgcg
     5221 atcacggtct acacggtcgc gctcggcctc ctgatcggag ggtttatcca ccatctcttt
     5281 tcataactgg tgtagcgatg tacatctacg agaccggtat cccgctgatc gacggggatt
     5341 atgggggtat gcatgccgcg accaatctcc tcctccttgc ccctccacta tcccacgcag
     5401 agcacctggc ataccggatc gcaagcccaa gacccgggga atggacggtt gcactctcga
     5461 ccgacgagcg cgccgccgat gttgtcggtg cgttccgcaa actgggagcg gagaggaacc
     5521 ggctcggggt tatcgatgcg gtcacgaagg gttcggtgcc gaccgtgcgg gacaccccga
     5581 aggtgaagtt cgtcacgagc ccccttgacc tgaccagcat ggggatcaag ttttcccgga
     5641 tggtggagga tatgtggaag gagggggcga tggccgatcc cgagggtcca atgcccccgc
     5701 cggttcggtt ctgcctcaac tcggtctcga cgatccttat gtatgcgcaa ctcgaggtga
     5761 ccttcaggtt tctccatgtc atcaccaacc gggtgaagaa actggagggg gtaggtgtct
     5821 acacccttaa cagcgagtcg tttgacaggc ggacggtgtc caccatcaag cagttgatga
     5881 gcatggtgat cgaggtgagg acggacgagg ggagagtagc ggcggaccac gatttccggg
     5941 taatcgggat ccatggcagg acgactccct ggatccgcta cttctacagc gacggcgtgg
     6001 tgacgataga ggaatgatgt tcggcaaccc tctgctgcaa ggagcggata cgcggtgctg
     6061 acgtttgttg gccttggcct ctacgacctc ggggatatca cgctaaaggg tcttacatac
     6121 gttaaatccg ctgatgtcgt cttccttgag gcctacacat cgcggttgat ggggacggat
     6181 ctcgccgcga tggaggtatt cttcgggaag gagatccggg ttctctcacg ggatgatgtc
     6241 gagcaggacc ctgccgagat cctggaacgc gccgctgccg gcagggtggc gttccttatc
     6301 gggggcgacc cgatggtctc gacgacgcac gccgatctcc ggatacgggc ggccgctgcc
     6361 ggtatagaga cctccatcat acacgcagcc tccatatcaa gcgcggtctg cggtctatcc
     6421 gggctgcaga actacaggtt cgggaagtcc tgttctgtcc ccttcccggc gagggggtgg
     6481 ttcccgacga cccctatcga tacgatcacg gtgaacctcg ggctgaacct ccacacgctt
     6541 gtatacctgg atatccagga cggccgctgc atgcgtatcc cggaggcgat tgagatcatc
     6601 gaggagatgg cggagaggcg cggggtcgaa ccgcccgcgc tgtatgtggg gatcgcccgg
     6661 gcgggttcgg agcacccggt ggttgctgcc gggagcggca gcaagctcaa ggagaccgat
     6721 tttggccccc cgctccatgt cctggccgtc ccggcggacc tccacccggt ggagcgggag
     6781 tacctggaga cctttgctgg cctatgaccc tggaggagta tgggggcctc tttgagggcg
     6841 ctctcctgcg ggtgcggatc gccgtcccgg aggaaagacc gctctttcgg gtggcggcgg
     6901 aggtgatgga gatggctgcg gcctacctga gcgacggggc ggcgtttctt gaggccggcg
     6961 accgggtgaa tgcgcttgcc gcctttgcct acgggcttgg ctggcttgat gccggttcga
     7021 gcctcgggct gatcgaaccg tatgatcccc acccgccgga agcggtggac gcacgcatcc
     7081 ttccctcgca gaccgcccgc ctggacgaga agacccggcg gtaccgccgt atgctcgatt
     7141 ccgcgcttga gggggtcgaa gaggcaccgg acgtctcaag cccgctccac gctgcagcga
     7201 gggggtttct gtcggtggcg cagaggtggc atgaggaggg cggagagcgt ctcgatgcgg
     7261 gtgatttcaa cggcgccctt ctccggctga gctacgggta tgcctggctt gatgccggca
     7321 tccgggcggg gctcttccgg gtgaccggtc gccgggatct atttaccgta tagacggtct
     7381 tttgaatgga caggtggtag ggaatcacca ccgggggttg ctggaggatc ttccccatct
     7441 gtctcttcgt atcagccgga tgctctcttt caaacggccg gcccccgggc gggtatttac
     7501 gccatacacc agcggtgaac caccaccgca agttaaaata gtatccagat aataccctat
     7561 tggttcaatt tcgggtgttt cggcccggga ttatccaaaa atccacaaat cgatctgtgg
     7621 atctcggtgg ggttccgggc caggtctcca gagcccgccc gtggagcaat ggagcatgaa
     7681 taggtctcag gtaaagtggc tgcttgtctc gatcggtttt agcgttcttg ttctgggcgt
     7741 tgtcctctac tttacagttg atgataccac ggttgattac atccgtcgga taaacccgtt
     7801 ctacctcctc gccgcggtcg tcctccacct cctctccctt gtcttctggt cgttgcggat
     7861 ccagaagatg tcggggtcgc tcgggtttcg ggtgcggttt acacactgca tgaacctggt
     7921 ccttgcaaac atgctcgtag ctgcggtaac accatcgcag gctggcggcg agcccgtccg
     7981 gattcatgaa ctctaccgcg cgggcgtccc ggtgggggac gccaccgccg ttgtcatcat
     8041 ggaacgcgtc ctcgacggga tcgtcctcgg cgcccttggt gtcttctcgg ttctctttct
     8101 cggaaaatac tggcgcagcc tcaccacggg gttcagcggt atgggtgtcc tgattggcgt
     8161 tgcctgtgct gtcacaaccc tcatcgtgat ttttttcgtc tactcggtca ggaaccccga
     8221 ctgccttaaa cgggcgctca ggtggggctc acgctgggtc gatcgccgct ggcaccttaa
     8281 gcggctcgaa caccttcttg aagcgatcga ccgcgaggtg gacaacttca accaggcgct
     8341 taccaggttt atcggccacg gcaagggcgg tcttttctgg ggtctggtct ttagcctgct
     8401 cttctggctt tcggagtttg tcatcgcatc gttcctcctc atgggccttg gccagccgcc
     8461 gcacttcatc gagtcgttca tcgtccagat cctcgttgcc atcatcatga tgatcccgct
     8521 caccccgggg agttcgggta ttgcggaact cagcgccacg tcgttctaca gcctctttgt
     8581 tccctcctcg atcgtgggta tcttcgtcct cctctggcgg ctgatactct actacctcaa
     8641 catcatcctg ggcttactcc cgggtttggt gattgtccgg cgtgaggtca tcgcccgggc
     8701 cagaaaacac cgttgataga cgtcaagata taaacgtaag gagagtgatc tcagataagc
     8761 agatgacagc tcaagaatgc agggtgcagg gggtattcat ggcggtgagc gatatgggag
     8821 cggcgccgac cgttctcctt gataccggcg gtgagagcat ggtccccatc tacatcgggc
     8881 tctgggaggc catctccatc agcaacgcgc tcaacagcga agttcttcca cggccgatca
     8941 cccatgacct catcatagag gtcttccgga acttcgagat agccctcgac gccctccaca
     9001 tcgatgcctt ggagggaggc gtcttctacg ccaaactcat gctgcggcag ggttcacgca
     9061 ccgagatgat ggactgccgg ccaagtgacg ggatagccat cgctcttcga tacccggcgc
     9121 cgatcatgat cgaagacacg gtcattgagg cggcggccgt aaaaaaagcc gacctaccac
     9181 aactggtcga tctgaaggaa tatctctgac cgctccggtc tcacttccgg cgtgcggcaa
     9241 gttcgtcgag cacgtcagcg ggatcgatgc ccaacgcctg gaacgctaca agcaggtgga
     9301 agaggagatc ggcggcttcg gataccgttc tctcaggtac ctggttcttt gcggccagta
     9361 tgaactcgac cgcctcttcg ccgaccttct ccagggattt gtcgacccct ttgcggtggg
     9421 tcaggatgga actgacgtaa ctgccggtcg aggggtggtc agcccggtcc tggatgacct
     9481 cccagagttc ctcgaggatc gcccagtcac gcatgggccg cctctcctgc gagcacttct
     9541 ccgagttcgg catcgaagta gcgggagagg agattccgcg ccttctcgat ggtgcacccg
     9601 cccttgccta tggcgatgcc gaggtcgccc ttgctgacgt agaccgtgat cttgccgtcg
     9661 tctcttcgtt cgacaccggt gacctcggcc ggtttgaaga cattctttgt gaatcgctcg
     9721 agctcgggtg agtattcaac catctcaaca cgttttccaa gaacccgctg catcttgcgg
     9781 atgttgctcc ccttcctgcc tatggcaaga cccatgtctc cttcttttat gaggtatact
     9841 atgcggtcga agcgctcgtc gattatgcag tccagagcgg tcgcccgggt gagtattctt
     9901 aactcttcga tgtatcgtcg ctctttaaag gatatggttc gtaccattga attagtcccc
     9961 aatgggaatg atgcggtaag ctatagtcac ttgggggtta tatacctgag tagcaggtca
    10021 ggcacgcctg ccggatctca tgtaaaaaag ggtctccggt atacaggacg gttttacttc
    10081 gtatttcgtg ccgggaagaa gggtgtctgg c
//
LOCUS       DAOSZX010000050         9448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6192_length_9448_cov_19.385926, whole genome shotgun sequence.
ACCESSION   DAOSZX010000050 DAOSZX010000000
VERSION     DAOSZX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_9448_cov_19.385926"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..2094
                     /gene="acsB"
                     /locus_tag="PK196_05400"
     CDS             58..2094
                     /gene="acsB"
                     /locus_tag="PK196_05400"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HON40887.1"
                     /translation="MNKIETDSLDAAIERGRTRLDARMQTLSGELGYPDTAYALPVTY
                     AITGIAVRDADEAREAYRQSGNNLLVACECLMAAAGAVEPPYTGFIPDTVLRQLGYTL
                     VDGSVIGLGLIVGKPESPDAVAAICREMQEKYLLTFLAGGVVEALSDAGVRLGLEYRL
                     VPLGPESARGIHFADILARIAMMFGGVKPGDAPALLSYAAERAKAFVIVFPGLTDEEI
                     AFVDSLRVLGMPILTVDGGYQDGEWIQVTSGEAVQTGMDLRGIRVTVTAIPIPMACSP
                     AFEGKSIRKEEMYVEFGGGRTPAFELLRMRPREEVEDGKVTVIGPEVDNVPAGSALPL
                     AILVDVAGKRMKPDYEPVLERRIHTFINYGEGSWHVAQRDIIWVRLSKDAVAKGVRIR
                     DIGILLAHKLKMEFPDHIEAIQVTLVTDRAKVEELLPQAREIYRRRDERIAGMKDDNV
                     EVFYSCTLCQTFAPNHVCIITPERPALCGAITWLDARIAHEITPSGANQPVEKGGVVD
                     ALRGEFAGVNRFVKKASHGETERVSLYSIMDYPMTACGCFECIAAIVPEVNGILIVNR
                     DHPGETPLGMTFSTLAGTIGGGAQTPGFLGIAKNYILSERFLQAEGGIARVVWMPTSL
                     KEELGDRLRERLAAAGIPEMYEKIADEVTAPTIEDLIALLERVEHPALAMKPLI"
     gene            2109..3020
                     /locus_tag="PK196_05405"
     CDS             2109..3020
                     /locus_tag="PK196_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HON40888.1"
                     /translation="MSKNSSDKPAIREVRLGATRAEGGTREVSYVIGGEEDLPFCGSD
                     PGRRLVALEICDDPAIWPPVVRNYVGDLANNPEDWAEAAERRYGADLVRLNFTSAKKR
                     GFDAFAEIASTTERVLAATTRPLIIEGSNDPQVDSEVFRRCGEAGEGERLLLGTAEAD
                     RYRSVAAAALAYGHLVIAQSPIDVNLAKQLNILLRETGVPEDRIVIDPYTGALGYGFE
                     YSYSVMERIRLAALAGDGDLAMPMISAPTDTLTIREVREAVPEEQDAMAVAWEFYTAY
                     SAFAAGASIVCVRHPLTVERLKKVLEA"
     gene            3031..4359
                     /gene="acsC"
                     /locus_tag="PK196_05410"
     CDS             3031..4359
                     /gene="acsC"
                     /locus_tag="PK196_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HON40889.1"
                     /translation="MKALDIYKHLPKTNCKKCGYPTCLAFAMKLAVGKADIEACPDID
                     PQAKALLGGVTRPPVRAVTIGAGERSVTVGEEFVMFRHEKTFYHPPGIMVEVPDTWPD
                     DEVRSVVEEVRETVVERVGQDLVLNGVAVRNESGDADRFAETVNLAGGGTSLPLVLIA
                     DDPPAFEAALAAAGQYRPLIHAATAENWEPLAALAKRYGSPLVVRAGTPADLAALAGN
                     CAGAGVADLLLDPAPGSLHGYIATATGIRRSAIERTEPALGYPIYLDAGTFKDTDAAV
                     AAGILKYAGVIVTPRLSRASREAALTLRQNIYTDPQHPIQVAPGVYPVNDPGREAPVL
                     LTVNFSLTYFTILGYLEASRMPCHLLIVDTEGLSVLTSVAGGKLTETMVADALKKFGV
                     ANLVDHRVLIIPGYAAPLSGKIEDATGWKVMVGPRDAADLPEYLEKEWKT"
     gene            4359..6281
                     /locus_tag="PK196_05415"
     CDS             4359..6281
                     /locus_tag="PK196_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HON40890.1"
                     /translation="MPTVTFLPGYRKVTVPEGASLLDAARAAGLAMNVVCGGQGKCGK
                     CQVFIKSGNVSFEREKFRKFFSDTEIARGACLACQATVLGDVLVDVPEGSLIQEQKIL
                     VEIPGREEIPLNPAVRKYEVYLSPPSLDDTTPDLTRLLEGVEAVGGPPPNRVYAPLGV
                     LRYLPQVLRRSDWHVTATVALVPGGYRLSSVDDGDTTDRIYGAAVDLGTTTIVVYLWS
                     LVDGQILSTASNYNRQISCGEDILSRVNFSRKGGLARLQQLAAESINEALKNAADAAG
                     IDLEEIFEVMVAGNTVMTHLFLGIDPGYMIAEPYIPVVQRMLSTAARRVGIIIHENAG
                     IYTFPAVSNFIGGDVIADILAAGMTEREEVTLLIDIGTNFEAVLGSRDWMLSCSGAAG
                     PALEGGEVLFGMRANPGAIERVQIDPYTLTPTYTTINGIAPRGICGSGLIDLLAELLR
                     ACVIDRTGGINLEIDHPRIRKGRYAPEFVVVWKEETPIGKDIVITENDIKNLIMSKAA
                     IHGACMTLLDAAGLTPRDIEKICLTGAFGNYINKENAITIGLIPDVPLERVENIGNGA
                     ITGANIALVNRKRRKELDGLARKITYIELNADPSFMDRYTSSCFLPHTDLSLFPRVQQ
                     MLEACRLRQGVNSRQG"
     gene            6242..7303
                     /locus_tag="PK196_05420"
     CDS             6242..7303
                     /locus_tag="PK196_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40891.1"
                     /translation="MQAEAGGELTAGMIPSPELNATGVGALPHRDPEEACRAVLAVFP
                     RIPYVPTLPNRGLLEGIVFADSEHLPGGVIRDGRLVLDRSIDPSEAMERILLDYIERN
                     AEPYAVGRDYGSGFHAMMAHDLSDALLVKCQATGPVTFGMQVVDEARRPILYDPEYAD
                     LLGKLLGLRARWCEIAMRERMMARETLVVLNEPYLASLGSAVVPVDAEVAASAFQDIA
                     ALLDGGLGIHCCANTDWSFVLGLRPALISIDAWTYAREFLLYLDEIAAYMEDGGVVAW
                     GIVPADYTVFAAEGQDSFFSRFQAIRARATGIIDPDLFDRQSMITPTCGIRTAGEREA
                     VAIMEAAASLSRRLRGEEA"
     gene            7300..>8022
                     /locus_tag="PK196_05425"
     CDS             7300..>8022
                     /locus_tag="PK196_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON40892.1"
                     /translation="MTRPCTVVISGKGGTGKTTISALLIDALVRAGERPVLAVDADPN
                     ANLHEALGITLNETLGGMREEAFTRSIPPGMDRTGYIKYRFRRALVEAEGYDLLAMGR
                     PEGSGCYCFPNALLKECIETLERGYRYVVVDSEAGMEHIARGTIRRPDILLIASDPGA
                     RGIRTAGRIRDLAESLGLEREKIFLVVNRDHGDAVIESDLPLIARIPEDPAVDEADLA
                     GTPIAEIPDESPARVAVRDLAAR"
     assembly_gap    8023..8122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8207..8599)
                     /locus_tag="PK196_05430"
     CDS             complement(8207..8599)
                     /locus_tag="PK196_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON40893.1"
                     /translation="MVELQDISRLIEEGNTYLPGQKSGTRSAYYSNPDWSGVRLLDLA
                     SGADTGGAFSSHLVRVQKGCEVPDHLHENQWEWNAVLAGQGKMILDGREILFGPGDTF
                     TTPPGVRHTVVADKEDIALLAVFVPGTM"
     gene            complement(8636..>9448)
                     /locus_tag="PK196_05435"
     CDS             complement(8636..>9448)
                     /locus_tag="PK196_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HON40894.1"
                     /translation="QATKKVFMSTCITCPILRYNPGVVAQTFATLRQMYPKRVGVAVG
                     AGEAMNEVPVTGKWPSVAERQEMTVEAVQVMRKLWESDEPVTFKGKYFVLDKAFLYTK
                     PEDKTVPLYFSGMGPKGAKLAGMYGDHLMTVAADPATLKNVTIPKFEEGAREAGKDPS
                     KMEHAMLIWYSVDPDPDKAVEGNRFWAGCLVPSMFKYKVYDPKEVQLHANLVHCDTIK
                     ENYICATDAEGLIKGIERFKEAGIDHFCLGNSSPNVNYGIDIFKEVIPAVTE"
BASE COUNT         1795 a         2805 c         2969 g         1779 t
ORIGIN      
        1 cgccgctggg aacaaccttt tatgggcgcg taaggagtaa ctcatcctga tggcaccatg
       61 aataaaatag agacggactc gcttgatgcg gctatagaac gcggccgcac cagactcgat
      121 gcacggatgc agaccctctc cggcgaactt ggatacccgg ataccgcata cgccctcccc
      181 gtgacctatg ccatcaccgg gattgccgtt cgtgatgccg acgaagcgcg tgaagcatac
      241 cggcagtccg gcaacaacct tcttgtcgcc tgcgaatgcc tgatggccgc tgcaggcgcg
      301 gttgaaccgc catacacagg cttcatcccg gacacggttc tcaggcagct cggctacacc
      361 cttgtcgacg ggagtgtcat aggtcttggc ctcatcgtcg ggaagcctga gagccccgat
      421 gcagtcgccg ccatctgccg ggagatgcag gagaagtacc tcctcacctt ccttgccggc
      481 ggcgtggtgg aggctctctc cgatgccggt gtccggctgg gactcgagta tcggctggtg
      541 ccgctcgggc cggagagcgc tcgcgggatc catttcgccg acatccttgc caggattgcc
      601 atgatgtttg gtggggtgaa gcccggcgat gccccggccc tcctctctta cgccgcagag
      661 agggcaaagg catttgttat agtcttcccc ggtctcacag atgaggagat agcgtttgtc
      721 gactcactcc gggttctcgg gatgcctatc ctgactgtcg acggcggtta tcaggacggg
      781 gagtggatcc aggtcacctc cggtgaggcc gtgcagaccg gcatggatct ccgcggtata
      841 cgggtcacgg ttaccgcgat cccgatcccg atggcctgct cccctgcgtt tgagggaaag
      901 agcatccgca aagaggagat gtacgttgag ttcggaggcg gccggacgcc tgctttcgaa
      961 cttctccgga tgcggccgcg ggaggaggtc gaggacggga aggtaaccgt catcggaccg
     1021 gaggttgaca acgtcccggc aggctccgcc ctcccgcttg cgatcctggt ggatgttgcc
     1081 ggcaaaagga tgaagcccga ctacgaaccc gtgcttgaga ggcggatcca taccttcatc
     1141 aactacggtg agggctcctg gcatgtcgcc cagcgcgaca tcatctgggt caggctctcg
     1201 aaggatgcgg tggcaaaagg tgtcaggatc cgcgacatcg gtatactgct tgcccacaaa
     1261 ctcaagatgg agttcccgga tcacatcgaa gcgatacagg tgacgcttgt aaccgacaga
     1321 gcaaaggttg aggagctgct cccgcaggcg cgcgagatct acaggagacg cgacgaacgg
     1381 attgccggca tgaaggatga caacgtcgag gtcttctact cctgcaccct atgccagacg
     1441 tttgctccaa accacgtctg catcatcacc cccgaacgcc cggcactatg cggcgcgatc
     1501 acatggctcg atgccaggat tgcccacgag atcacgccct ccggggcaaa ccagcctgtt
     1561 gaaaaggggg gagtggtcga cgctctccgc ggcgagtttg caggtgtgaa ccggtttgtg
     1621 aagaaggcgt ctcacggcga gaccgaacgg gtctcgctct acagcataat ggactacccg
     1681 atgaccgcct gcgggtgttt tgagtgcatc gcggctatcg tccccgaggt gaacggtatc
     1741 ctgatcgtga accgtgacca ccccggggag acgccgctcg gtatgacctt ctcgaccctt
     1801 gccgggacaa tcggcggcgg tgcgcagacc cccgggttcc tcgggatagc aaagaactac
     1861 atcctgagcg agaggttcct gcaggccgag ggcggtatcg cgcgggtggt ctggatgccg
     1921 acctccttaa aggaggagct cggcgaccgg ctgcgggaga ggcttgcagc agcagggatc
     1981 ccggagatgt atgagaagat cgcggacgag gtcacggcgc cgacgatcga agacctgatc
     2041 gcgctcctcg agcgggtcga gcacccggcg cttgccatga aaccgctgat ctgaggaggg
     2101 agttatttgt gagtaaaaac agttctgata aaccagccat ccgggaggtg cgcctgggcg
     2161 caacacgcgc cgagggcgga acccgggagg tatcctacgt tataggcggc gaagaggatc
     2221 tgccgttctg cggcagcgac cccggccgac gccttgtggc actagagatc tgcgacgatc
     2281 cggcgatctg gccgccggtc gtccgcaact acgtgggcga ccttgcaaac aaccccgagg
     2341 attgggcaga ggcagcggag cgcagatacg gtgcagacct tgtgcggttg aacttcacca
     2401 gcgcaaaaaa gagagggttt gatgcgtttg ccgagatcgc ctcgacaaca gaacgcgtgc
     2461 ttgcggcaac gaccagaccc ctgataatcg agggcagcaa cgacccccag gttgacagcg
     2521 aggtcttccg ccgctgcggt gaggcgggtg agggcgagcg tctcctcctc gggacagcgg
     2581 aggccgacag ataccggagc gtcgccgcgg cggcgctggc atacgggcat ctggtgatcg
     2641 cacagtcgcc catcgatgtc aacctggcaa aacagctaaa catccttctc cgggagacgg
     2701 gcgtgcccga ggaccggatc gtgatcgacc catacacagg ggcgctcggc tacgggtttg
     2761 agtactccta ctcggtgatg gagcggatac ggcttgccgc gctcgccggc gacggcgacc
     2821 tggcgatgcc gatgatcagc gctcccaccg acaccctcac catccgggag gtgcgggagg
     2881 ccgtaccgga ggagcaggat gccatggcgg ttgcatggga gttctatacc gcatactccg
     2941 cctttgccgc cggggcttcg atcgtctgtg tccgccatcc gctgacggtg gaaaggctca
     3001 aaaaggtgct ggaggcctga tccgatggca atgaaagcgc ttgatatcta taagcacctg
     3061 ccgaagacga actgcaagaa gtgcggttac ccgacctgtc tggccttcgc catgaaactt
     3121 gccgtcggaa aggcggatat cgaggcctgc cccgacatcg acccacaggc aaaggcgctt
     3181 cttgggggag tgaccagacc gccggtgagg gcggtcacga tcggtgcggg cgagcgatcg
     3241 gtcacggtcg gcgaggagtt tgtcatgttc cggcacgaga agaccttcta ccacccgccc
     3301 gggatcatgg tggaggtccc cgacacctgg ccggatgatg aggtgcgatc cgtggtggag
     3361 gaggtgaggg agacggtcgt cgagcgtgtg ggccaggacc tcgtcctcaa cggtgtggcg
     3421 gtgagaaacg agagcgggga cgccgaccgg ttcgcagaga ccgtgaacct cgccggcggc
     3481 ggaaccagtc ttccgcttgt gctgatagcc gacgaccctc cagcctttga ggcggccctt
     3541 gcggcagcag ggcagtatcg gccgctgatc cacgcggcga ccgccgagaa ctgggaaccg
     3601 ctggccgcgc ttgctaaacg gtatggctcc cctcttgtcg tccgggcggg gacgccggcc
     3661 gacctcgccg cgcttgccgg aaactgcgcc ggcgcggggg tcgccgacct gctgctcgac
     3721 cccgcaccgg ggtcgctcca tggttatatc gccaccgcga ccgggatccg gaggagtgcc
     3781 atcgagcgga cggagcccgc gctcggctac cccatctacc tggacgcagg aacattcaag
     3841 gataccgatg cagcagtcgc tgcgggcatc ctcaagtatg cgggggtcat cgtcaccccg
     3901 cggctatccc gcgcatcgag ggaggcggcg ctcaccctgc gtcagaacat atacaccgac
     3961 ccgcagcacc ccatccaggt ggcacccggg gtctacccgg tcaacgatcc cggacgggag
     4021 gcgcccgtcc tcctcacggt gaacttctcg ctcacctact tcacgatcct tggctacctc
     4081 gaggcgtccc ggatgccctg ccacctcctg atcgtggata cggaagggct ttcggttcta
     4141 acatcggtcg ccggcgggaa actgacggag acgatggttg ccgatgctct caagaagttc
     4201 ggcgtcgcca acctggttga ccaccgtgtc ctcataatcc ccggttacgc cgctccgctc
     4261 tccgggaaga tcgaggacgc aaccggctgg aaggttatgg tcggaccccg cgatgcagcc
     4321 gatctccctg agtaccttga gaaggagtgg aagacctgat gccgacggtg acgttcctcc
     4381 ccggctaccg gaaggtgacc gtccccgagg gggcgagcct tcttgatgcg gcacgcgcgg
     4441 ccggtcttgc gatgaacgtg gtctgcggcg ggcaggggaa gtgtgggaag tgtcaggtct
     4501 tcataaagag cgggaatgtc tcgtttgaac gcgaaaaatt ccgcaaattc ttctccgaca
     4561 cagagatcgc gcggggtgcc tgccttgcct gccaggcgac cgttctcggt gacgtcctgg
     4621 tggatgtccc ggaaggatcg ctcatccagg agcagaagat cctggtggag atccctggca
     4681 gggaggagat cccgctcaac cctgccgtca ggaagtacga ggtctacctc agtccaccgt
     4741 cgctcgatga caccacccct gaccttacca ggctgctgga aggggttgaa gcggtgggcg
     4801 gaccgccgcc aaaccgcgtc tacgcgccgc ttggtgtcct ccgctacctc ccgcaggtgc
     4861 tccggcggag tgactggcac gtgaccgcca cggtcgcgct cgtgcccggc gggtatcgac
     4921 tgagcagtgt ggatgacggc gacaccaccg atcggatata cggcgcggcg gtcgacctcg
     4981 ggacgacgac gatcgttgtc tacctctgga gcctggtcga tggccagatc ctctccacgg
     5041 cgtctaacta caaccggcag atctcctgcg gcgaggatat cctctcaagg gtgaacttct
     5101 ccaggaaggg cggtcttgca cggctgcagc aactcgcagc ggagagtatc aatgaggcgc
     5161 tgaagaacgc cgcagatgcc gccgggatag atcttgagga gatctttgag gtgatggttg
     5221 ccggaaacac cgtgatgacc catctctttc tcgggatcga ccctggctac atgatcgccg
     5281 agccctacat cccggttgtg cagcggatgc tctcgacggc cgcccgccgg gtcggtatca
     5341 ttatacacga gaatgccggg atatacacat tccccgcggt ctcgaacttc atcggcggcg
     5401 atgtaatcgc cgatatcctg gccgccggca tgacagaacg cgaggaggtc accctcctga
     5461 tagatatagg cactaacttc gaggccgtgc tcggcagccg tgactggatg ctctcctgct
     5521 cgggtgctgc cggcccagcc ctcgagggcg gagaggtgct gtttggtatg cgcgcaaacc
     5581 ccggtgcgat cgagcgcgtc cagatcgatc cctatacgct cacaccaacc tacacaacca
     5641 tcaacggcat agccccccgg ggtatctgcg gttctggact tatcgatctc ctcgcagaac
     5701 tgctccgtgc ctgtgtcatc gaccgaacag gcgggatcaa tcttgagatt gatcaccccc
     5761 ggatccggaa aggacggtat gcccctgagt ttgttgtggt ctggaaggag gagacaccga
     5821 tagggaagga tatcgtcatc accgagaacg acatcaaaaa cctcatcatg agcaaggccg
     5881 cgatccacgg tgcctgcatg acgctcctcg atgcagcggg gctgacgccg cgcgatatcg
     5941 agaagatctg cctgacgggg gcgttcggga actacatcaa caaggagaac gccatcacga
     6001 tcgggttgat ccctgatgtg ccccttgagc gtgtggagaa tatcgggaac ggcgcgatca
     6061 cgggtgcaaa catcgccctg gtcaaccgca aacggcgtaa ggaactcgac ggccttgcaa
     6121 ggaagatcac atacatcgaa ctgaatgccg acccctcctt catggaccgc tacacatcga
     6181 gctgtttcct gccgcacacc gacctttcgc tattcccgcg cgtgcagcag atgcttgagg
     6241 cgtgcaggct gaggcagggg gtgaactcac ggcagggatg ataccctccc ccgaactcaa
     6301 tgcaacgggt gtcggggcgc tgccccaccg cgacccggaa gaggcctgcc gggcagtgct
     6361 tgcggtcttt ccccgtatcc cctatgtgcc cactctgccg aaccgggggc tcctggaggg
     6421 gatagtcttt gcagactccg agcacctccc cggcggcgtg atcagggacg gcagactcgt
     6481 tctcgaccgg agcatcgatc cctctgaggc gatggagcgg atcctgctcg actacattga
     6541 gaggaacgca gagccctacg cggtcggcag ggactacggt tcggggtttc atgcgatgat
     6601 ggcccacgac ctctcggacg cgctcctggt caagtgtcag gcgacaggtc cggtcacgtt
     6661 cgggatgcag gttgtggatg aggcgcgccg gcccatcctc tacgacccgg agtacgccga
     6721 cctgcttgga aaactcctgg gtctgcgggc gcggtggtgt gagatcgcga tgcgcgagcg
     6781 gatgatggcg cgtgagacac tggtggtgct caatgaaccc taccttgcct cgctcgggtc
     6841 agcggtcgtc ccggtggatg cggaggtcgc agcgtctgcg ttccaggata tcgccgctct
     6901 cctggatgga gggctcggga tccactgctg cgcaaacacc gactggagtt ttgtcctggg
     6961 tctccggccg gccttaatct cgatcgacgc ctggacgtat gcacgtgagt ttctgctcta
     7021 cctcgatgag attgcggcat acatggagga tggcggggtt gttgcctggg ggatcgttcc
     7081 ggccgattac accgtcttcg ccgcggaagg tcaggactcg ttcttctcga ggttccaggc
     7141 gatccgcgcc cgtgcaaccg ggatcatcga tcccgacctc tttgaccggc agtcgatgat
     7201 cacaccgacc tgcgggatcc ggaccgccgg cgagcgtgag gctgtcgcga tcatggaggc
     7261 ggccgcttcg ctctcccgcc gcctgcgggg tgaggaggca tgacccggcc gtgcaccgtt
     7321 gtcatatccg ggaaaggggg gactgggaag accacgatat cggcgctcct gatcgatgcc
     7381 ctggtcaggg caggcgaacg tccagtcctt gctgtggatg ccgatccaaa cgcgaacctt
     7441 cacgaggctc tcgggatcac gctcaatgag acgctggggg gcatgcgtga ggaggccttc
     7501 acccgctcca tcccgccagg catggacagg acgggctaca tcaaataccg cttccgccgg
     7561 gcgctggtgg aggcggaggg ctacgacctt cttgccatgg ggcggcctga ggggtcggga
     7621 tgctactgtt tcccaaacgc gctcttaaag gagtgcatag agacgctcga acgcggctac
     7681 cggtatgtcg tcgtggactc cgaggcgggt atggagcaca tagcccgagg gaccatcagg
     7741 agaccggata tcctcctgat agcaagcgat cctggagccc gcgggatccg gaccgccggc
     7801 cgcatacgcg accttgcgga gtcgctcggt cttgagcgtg agaagatatt cctggtcgtc
     7861 aaccgcgacc acggggatgc tgttatcgag agcgatctcc cgcttatcgc ccggatcccg
     7921 gaggacccgg ctgtcgatga ggcagacctt gcgggaacgc cgatcgcgga gatacctgac
     7981 gagagtccgg cgcgggtggc ggttcgtgac cttgccgcgc ggnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnaatgccga tatttctggc agagaggtat tgaagctgcg
     8161 ggatttctgt gttatatggg gaatgagacg gattttatcg ggcttctcac attgtgccgg
     8221 gaacgaagac cgccagcagg gcgatatcct ccttatcagc gacgacggta tgccggacgc
     8281 ccggcggtgt ggtgaacgta tctccgggcc caaagaggat ctccctccca tcgaggatca
     8341 tctttccctg ccccgcgagg acggcgttcc actcccactg attctcgtgg agatgatcgg
     8401 gaacctcaca acccttctga accctgacaa ggtgagatga gaatgcgcct cccgtatccg
     8461 cccctgatgc gagatccagc agtctgacac cgctccagtc ggggtttgag tagtatgctg
     8521 accgggtgcc gcttttctgt ccgggtaagt atgtgttgcc ctcctcaatg agcctggaaa
     8581 tatcctgaag ttctaccata atcctccaaa ataaattttt gggggcgagc cccgttcact
     8641 ccgtgacagc agggataact tccttgaaga tatcgatgcc gtagttcacg ttcgggctgg
     8701 agtttccaag gcagaagtgg tcgatgccag cctccttgaa acgctcgatc cccttgatca
     8761 acccttcggc gtccgtggcg cagatgtagt tctccttgat ggtgtcgcag tggacaaggt
     8821 tcgcgtggag ctggacctcc ttcgggtcgt agaccttgta cttaaacatc gaggggacaa
     8881 gacatcctgc ccagaaccgg ttgccctcga ccgccttatc ggggtcgggg tcgaccgagt
     8941 accagatgag catggcgtgc tccatcttgc tcgggtcctt ccccgcctcg cgggctcctt
     9001 cctcgaactt tggaatggtg acattcttga gcgtggctgg atcagccgca acggtcatga
     9061 ggtggtcacc atacatgccg gcaagctttg cacccttggg gcccatgccg ctgaaataga
     9121 gcgggacggt tttatcctcg ggtttggtgt acaggaacgc cttgtcaagg acgaagtact
     9181 tgcccttgaa ggtgaccggt tcgtcgcttt cccagagttt gcgcataacc tggacagctt
     9241 cgaccgtcat ctcctggcgt tctgcaacgc ttggccactt gccggtgaca ggtacctcgt
     9301 tcatcgcctc accggcgcct acagcaacac ctacacgttt ggggtacatc tgtcggagcg
     9361 ttgcaaacgt ctgtgcaacg acaccagggt tgtatctcag gatcgggcag gtgatgcagg
     9421 ttgacatgaa caccttcttg gtggcctg
//
LOCUS       DAOSZX010000051         9338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6277_length_9338_cov_21.897986, whole genome shotgun sequence.
ACCESSION   DAOSZX010000051 DAOSZX010000000
VERSION     DAOSZX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_9338_cov_21.897986"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="PK196_05440"
     CDS             <1..1960
                     /locus_tag="PK196_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON40895.1"
                     /translation="HSGKHYMQKIEIKSHDYPIRVIPAVRCLSTATAALPAPVAEKPA
                     ILSTAGGGLLMRSSIQAGILAASSLAVIAITIACLANGITTVFTHIFYIPIVYAAYYY
                     LRRGVAVSAGLGAIYLALVLILTPATAGIIVAALIRVAVFIAIAVIVAALAARLRKAV
                     ATGLEEEKRLQELNREIHKREGWYRAIYEQSPIAIGVYDAEGKLTHANPAALDLLGVR
                     DLDSLIGFPLFEDPNFPEDQKKNLLEGRTTHYQAQFDFEMVRSHNLYPTSRRGVIWLD
                     RLITPIRDDGGSIIGYLVHAYETTARKQAEDALRESEEMFRTVAQSAYDAIVMVDSEG
                     RATFWNRAAVKMFGYSEDEVRGRPVHDLIAPADVLNAAEEGVRNFALTGTGPVIGRPL
                     ERTARRRDGKEFPVELTISPVIIGGAWHAVAVIRDVSERKEAEAALRKAHRQLQILTG
                     ITRHDILNSITAADGYMELYRDADPAARETYLEKAHQAIHKIQRQIEFTRQYEAIGTL
                     EPGWQNLEILIQGLEVPSSISFQTSGLKREIYADPMVKQVFENLLDNTLRHGGDGVSR
                     ITLTAEVSDGGDLLVTWEDDGVGVSGEDKKRIFERGFGKHTGLGLFLSREILGLTGIT
                     ITENGIPGSGARFVIRVPAGKWRDLEQG"
     gene            complement(2012..4117)
                     /locus_tag="PK196_05445"
     CDS             complement(2012..4117)
                     /locus_tag="PK196_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HON40896.1"
                     /translation="MAEEINVEVTDNVGEWTKFLKKQYRRELAEISREYPHRRSLVID
                     YRKILHNRLAFELLRSPGKVIGDIRDAIVQNKLLKLKDGQEPDHLNIRFTNLPQKTNI
                     RDIRADQINTFVSTEGIIRKTTEVRPRIVSAVFRCRSCNNLTDPIPQGYGRFDEPEFC
                     PKCERKTRLDLVINRCRFVDAQKLRIQESPEGLRGGEQPQTLDIDVIDDLTGLVAPGD
                     RVVVNGILRSVQRINYGQKSTLFDIYLEGNSLEVAEKEFEEVAITEEDEAKIMALARD
                     PMVYRKIVRSIAPTIYGSDDVKEAIALQLFGGIPKEMPDGSRLRGDIHVLLVGDPGIA
                     KSQILRYVAKLSPRGIYTSGKSSTSAGLTATAVKDEFGDGRWTLEAGALVLADMGVAA
                     VDEMDKMAKEDRSALHEAMEQQSVSVAKAGITATLKCRCALLGAANPKLGRFDQFIPI
                     AEQINMPPSLLSRFDLIFVMTDQPEAERDAAIAQHIIKTHAVGELIKQHEHEPLPGVD
                     DEYIRRALAPVTPDIDPVLLRKYIAYAKRTCFPILSDGAKEALIGYYMRLRNLASGDK
                     PVPVTARQLEALVRLAEASARMRLSNTIDTEDTDRILKIVDACLRQVAYDAESGSFDI
                     DKVVTGVTKSQRDIIRSIKEAIRNLSGESGGQARIEDVIDVLVQQGFARDKIEYTLDH
                     LKRGGEVLEPRRGLVKLIG"
     gene            complement(4131..5099)
                     /locus_tag="PK196_05450"
     CDS             complement(4131..5099)
                     /locus_tag="PK196_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HON40897.1"
                     /translation="MEGNHTIWIEKYRPRRLDDIVGQNDIVVRLKSYVKTGNLPHLLF
                     AGSAGVGKTTAAVALAREFFGDSWQTNFREMNASDERGIDVVRTQIKEFARTSPLGDA
                     QFKILFLDEADALTTDAQAALRRTMETYARTCRFILSCNYSSKIIDPIQSRCAIYRFR
                     PLDREAVIEEIRRIAAAEGLTLTEGALDAIVYVAAGDMRKAINALQGAAIISPEIDEK
                     MIYAITATARPDEIDELLDLSIGGRFDEAEKALSDLTRVRGIAPNEVLNQCYRALVQR
                     DIDRALKVKLIDAIGEADFRLSEGANSDIQMEALIARFVLAAAEHR"
     gene            5229..5927
                     /locus_tag="PK196_05455"
     CDS             5229..5927
                     /locus_tag="PK196_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40898.1"
                     /translation="MVPVERVFAREFSAARQTADGRILVTPSGACCRRLFIVGALTER
                     LGSGDLVHARVADPTGVFLVTADWHNPDALDTLRCIDPPAFVAVTGRPVFSGSGDGIC
                     ATLDAEAVRVADRGARDTWVLATAAATLDRLEALRDGRGEPAAILEMVRAAVETVRET
                     PSPVGITPRTLPEVMLDALQRMGGGSRGIGVDDAVAAGVRSGLTAGEARAALEELLAD
                     GECYQPVDGTIKLI"
     gene            5936..6673
                     /locus_tag="PK196_05460"
     CDS             5936..6673
                     /locus_tag="PK196_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON40899.1"
                     /translation="MRLHFIENGPALLVEQEQRVLVVADLHMGIESGLERCGVHITSR
                     SDARAKRVIACIEETRPDLLLLLGDLKHNVPVTTRQEYRELPRILQSFRELVPIAVTP
                     GNHDGGIDRFLQEGELLPAGGALIDGVGYLHGHTHPAPSLLGRLIILGHHHPVVSLMD
                     SVGCAARAQPAYLYSVFEEPTGERSRLLFVPAFNELAGGIDVGLLRESGLGPLSRCID
                     DKNAEVFLADGTYVGSPATLCPPDADG"
     gene            6618..>9338
                     /locus_tag="PK196_05465"
     CDS             6618..>9338
                     /locus_tag="PK196_05465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON40900.1"
                     /translation="MARTSAPRPRSAPQTPTAELLDPRVREVVRKRGFTEFSEAQEQS
                     IPRLLAGDNLILVAPTGTGKTESAMLPVFDRLLETPGPGFKALYITPLRSLNRDILAR
                     MEWWCRELSLTVGVRHGDTPQNERRKQALNAPDLLITTPESLQALFMGKRLREHLKNV
                     RYVIIDEIHDLAESKRGAQLAVALERLAEYAGEFQRIGLSATVGNPDEIGRFLCGARP
                     FSVVEVPVASNLDITVRFAGEDFGDQVRTVERCLDAPGSTLVFVNTRVTAEALGHSLY
                     SRGDVEVHHGSLSRDVRVDAEERFRNGEIRALIATSSMELGIDIGHIEHVLQFGSPRE
                     VSRLVQRVGRAGHRLDAVSRGTVLATGFDDLLESLVIARRAKANECERVVPPAGAADV
                     LANQVAAIAVEYGEIEISRLRKIVERSSIFAGCGSLLDDVCRQMAEHRLIRVEGTRVI
                     RTGRARRYLVGNLSMIHDERKMEVFDIVSRRTVGTLDESFVLGWMQTGAVFITKGQLW
                     RVLDMEGGRITVEPAARVRGEVPSWEGEQIPVPFAVAREVGALRRMRAFGRYSDVPGV
                     IGYAERFMARMDAGNHPVPSDRLITLENADEGVVCNVCGGHKANETLARALSILISAR
                     YGTTVGIEVGAYRFLLRLPGNVRALEVQEAMAGIEPEHLAGILRLALKRTALFKWKLV
                     QVAKKFGAIDADADYERFSIHRLIDLFDGTVIAAEAYRELFSRYMDVEAAEEILAGIR
                     DGRIAVTTGRLSPIGSEGLSSSRDTVPPPMIDQAVIGTLMRRLEKEEVVLFCMNCRKW
                     KSRTVVERVPDRPQCPLCGARLIAALKPWDEELIPAVTKKKKSEEERAIEVRFLRNAN
                     IVLSSGKKAVTALAAKGVGPETASRILATLSEGDAFYREILKAERNYVKTH"
BASE COUNT         1696 a         2926 c         2939 g         1777 t
ORIGIN      
        1 gcacagcgga aaacactata tgcaaaaaat agagataaaa agccatgact acccgatccg
       61 ggtcatcccc gccgtacgtt gtctctcaac cgcaaccgct gcgttgccgg cgcccgtcgc
      121 cgaaaaaccc gccatattat ccaccgctgg aggggggttg ttgatgcgga gcagcatcca
      181 ggccgggata cttgcagcct caagccttgc cgtgatcgcc atcacgatcg cctgcctggc
      241 aaatggtatc accaccgtct ttacgcatat cttctacatt cccatagtct acgccgccta
      301 ctactacctg cggcgcgggg ttgccgtatc cgccgggctt ggagcgatct acctggcgct
      361 tgttctcatc ctcaccccgg cgaccgccgg gatcatcgtt gcggctctca tccgggtggc
      421 ggtcttcatc gccattgctg tgatcgtggc cgcccttgcc gcccgcctcc ggaaagcggt
      481 cgccaccggg ctggaggagg agaagcgtct ccaggagttg aacagggaga tccataagag
      541 ggaaggatgg taccgggcga tatacgagca gtcgccgatc gccatcgggg tgtatgatgc
      601 agagggtaaa ctcactcatg ccaaccccgc cgcccttgac ctgctgggcg ttagggatct
      661 ggattcgctc atcgggtttc ccctgtttga agacccgaat tttccagagg accagaagaa
      721 aaacctcctt gaagggagaa caacccacta ccaggcccaa ttcgattttg agatggtcag
      781 gtcgcataac ctctatccca cctcacgcag aggggtcatc tggctggatc gcctcatcac
      841 ccccataagg gatgatggcg gctcgatcat aggttacctc gtccacgcct acgagaccac
      901 cgcacgcaaa caggcggaag acgctcttag agagagcgag gagatgttca gaaccgtcgc
      961 ccagtcggcg tatgacgcca tagtcatggt cgacagcgag ggacgggcga ctttctggaa
     1021 cagggcggcg gtgaagatgt ttgggtattc ggaggatgag gtgcgcggga gaccggtgca
     1081 cgacctcatc gccccggcag acgttctaaa cgcagcagaa gagggggtgc gcaacttcgc
     1141 cctcaccggc accggtcctg tcatcggcag gccgctcgag aggacggctc gcagaagaga
     1201 cggaaaggag tttcccgttg aactcaccat ctcccccgtg atcatcggcg gggcatggca
     1261 cgctgtggct gtcatccggg acgtcagcga gcgaaaagag gcggaagcag cgctccggaa
     1321 ggctcaccgg cagttgcaga tcctcaccgg gatcacccgc cacgacatcc tgaacagcat
     1381 cacagcggct gacggttata tggagttgta cagggacgcc gacccggccg cccgagagac
     1441 ataccttgag aaggctcacc aggcaatcca caagatccag cgtcagatcg agttcacccg
     1501 ccagtacgag gccatcggca cactggaacc gggatggcag aacctcgaaa tcctgatcca
     1561 gggcctggaa gtgccgtcat cgatctcgtt ccagacctcc gggctcaaac gcgagatcta
     1621 cgcggatccc atggtaaagc aggtctttga gaacctcctc gacaacaccc tgaggcacgg
     1681 cggcgacggc gtcagcagga tcaccctcac cgccgaggtc tccgatggcg gtgacctgct
     1741 ggtgacctgg gaagacgatg gcgtgggtgt ttcaggggag gataagaaac ggatattcga
     1801 gcgcgggttt gggaagcaca ccggtttggg cctctttctc tcgcgcgaga tcctggggct
     1861 gaccgggatc acgatcaccg agaacggcat ccccggcagc ggagcaaggt tcgtgatccg
     1921 cgttcccgcc gggaaatggc gggatctgga gcaggggtga ttggcccgcc accctcccgg
     1981 aaacgggatc tcaagggatc gcctcccacc cctacccgat caacttcacc agcccgcgcc
     2041 ggggctcgag cacctcgccg ccccgtttca ggtggtccag ggtatactcg atcttgtcgc
     2101 gggcaaaccc ctgctggacc aggacgtcga taacatcctc gatcctcgcc tggccgccgg
     2161 actcgccgga gaggttccgg atcgcctcct ttatagaccg gatgatgtcg cgctgcgact
     2221 ttgtgacccc cgtcacaacc ttgtcgatat caaaactccc gctctccgca tcgtatgcaa
     2281 cctgccgcag acaggcgtcc acgatcttga ggatccggtc agtgtcctcc gtatcgatgg
     2341 tgtttgagag acgcatcctg gcgctcgcct ccgcaagccg caccagcgcc tcgagctgtc
     2401 tggcggtgac cgggacaggc ttgtccccgc tggcaaggtt gcgcagacgc atgtagtatc
     2461 caatcagcgc ctccttcgcc ccatcggata gtattgggaa gcacgtcctc ttagcatagg
     2521 cgatgtactt gcgcagcagc accggatcga tatccggtgt gaccggcgca agcgcccgcc
     2581 ggatatactc gtcatcaaca cccggcagcg gttcgtgctc gtgctgcttg atcagttcgc
     2641 caacagcgtg ggtcttgatt atgtgctggg cgatcgctgc atcccgttcg gcctccggtt
     2701 gatccgtcat gacaaagatc aggtcaaacc gcgagagaag cgatggcggc atgttgatct
     2761 gctcggcgat agggatgaac tggtcaaacc ggccgagttt cgggtttgca gcgccaagga
     2821 gcgcgcaccg gcatttcagg gtcgctgtta tcccggcttt ggcaaccgag acggattgct
     2881 gctccatcgc ctcgtgcagc gcgctccgat cctcctttgc catcttgtcc atctcatcga
     2941 ctgccgcgac ccccatatcc gcaagaacca gcgcacccgc ctccagcgtc cagcggccgt
     3001 cgccgaactc gtccttcacc gccgttgccg tcagccctgc ggacgtcgac gacttcccgc
     3061 ttgtgtagat cccgcggggc gagagtttcg caacgtatcg cagtatctgg ctcttcgcta
     3121 tacccggatc cccaaccagc aggacatgta tatcgccgcg gagacgggag ccgtccggca
     3181 tctcctttgg tataccgccg aagagctgga gggcgatagc ctccttcaca tcgtcggagc
     3241 catagatggt cggcgcgatc gaacgcacaa tcttcctgta gaccatcggg tcgcgtgcga
     3301 gggccatgat cttcgcctcg tcctcctcgg tgatcgcaac ctcctcaaac tccttctcgg
     3361 cgacctcgag ggagttgccc tccaggtaga tgtcaaagag ggtgctcttc tggccgtagt
     3421 tgatccgctg caccgaccgc aggataccgt tcacaaccac ccggtccccc ggcgcgacca
     3481 gcccggtgag gtcgtcgatg acgtcgatat cgagcgtctg gggctgctcg cccccgcgga
     3541 gcccctccgg cgactcctgg atcctgagtt tctgggcgtc gacaaaccgg catcggttga
     3601 tgacaagatc gagtcgcgtc ttgcgctcgc atttcgggca gaactcaggt tcatcaaacc
     3661 ggccgtaacc ctgcgggatc ggatccgtca ggttgttgca ggaacggcac cggaatacag
     3721 cgctgacgat ccggggacgc acctccgtcg tctttctgat gatcccctcg gtgctgacga
     3781 atgtgttgat ctggtcggcc cggatgtccc ttatgttggt cttctgcggc aggttcgtga
     3841 accggatgtt gaggtggtcc ggttcctggc cgtccttgag tttgagcagt ttgttctgga
     3901 cgatcgcgtc ccgtatatcc cctataacct ttcccgggct gcgcaggagt tcaaaagcga
     3961 gccggttgtg aaggatcttg cggtaatcga tgactagcga acgccgatgg gggtactcgc
     4021 gcgatatctc ggcaagctcc cggcgatact gtttctttaa gaacttcgtc cactcaccga
     4081 cgttgtcggt gacctcgacg tttatctcct cggccacacc agacctcctt tcagcggtgc
     4141 tccgcggcgg caaggacgaa acgggcaatc agcgcttcca tctggatatc gctgttcgcc
     4201 ccctctgaga gccggaaatc ggcctcgccg atggcatcta taagtttcac cttgagcgcc
     4261 cggtctatat cgcgctggac gagcgcccga tagcactggt tgagaacctc gtttggggct
     4321 atgccacgta cacgggtcag gtcggagagg gccttctccg cctcatcgaa cctcccgccg
     4381 atcgagagat ccaggagttc atcgatctcg tccggtcttg cggtcgcggt gatcgcgtag
     4441 atcatcttct catcgatctc ggggctgatg atggcagcgc cctgcagagc gttgatcgcc
     4501 tttctcatat ccccggcggc gacgtagact atggcatcaa gcgccccctc cgtgagtgtg
     4561 agcccctcgg ccgcggcaat cctcctgatc tcctctatga ccgcctcccg gtcaagcggc
     4621 ctgaaccggt atatggcgca cctgctctgg atagggtcga tgatctttga ggagtagttg
     4681 caggagagga tgaaccggca tgtgcgggca taggtctcca tcgtcctcct gagagccgcc
     4741 tgcgcatcgg tggtgagcgc gtccgcctcg tccaggaaga gtatcttgaa ctgcgcatcg
     4801 ccaagcggcg acgtcctggc gaactccttg atctgggtcc tgacgacgtc gataccgcgt
     4861 tcgtcggagg cgttcatctc ccggaagttc gtctgccagg agtcgccgaa gaactccctg
     4921 gcaagcgcca cggcggcggt ggtcttcccg acccctgcgc tgcctgcaaa caggaggtgc
     4981 ggcaggttgc cggtcttaac gtaagacttg aggcgcacca cgatatcatt ctgcccgact
     5041 atatcgtcaa gccgtctggg ccggtacttc tctatccaga tggtgtggtt gccttccatt
     5101 tgcataggta taggtgccag tccatcgtaa aagattcggg ttcacggtga tcccttttag
     5161 tgggggaaaa acagataata ggtacagacg cgttatatca caaccgcttt ctcaaacgga
     5221 ttgattgcat ggtgcctgta gagcgtgtat ttgcgcggga gttctccgcc gcgcggcaga
     5281 ccgccgacgg ccgtatcctg gtgacgccgt caggtgcatg ctgccgccgc ctcttcatag
     5341 tcggtgctct caccgaacgc cttggaagcg gggatctcgt ccatgcccgt gtagccgatc
     5401 caaccggtgt cttcctggtc acggcggact ggcataaccc cgacgcgctc gatacgctca
     5461 gatgcatcga cccgcccgcg ttcgtcgccg tcaccggccg accggtcttc agcggttcgg
     5521 gagacggtat ctgcgcaacg ctggatgctg aggccgtccg cgtggccgac cgcggcgccc
     5581 gcgacacctg ggtgctcgca accgccgccg caacgctcga ccggctcgag gcgctccggg
     5641 atgggagggg tgagcctgcc gcaatcctgg agatggtaag ggcggcggtt gagaccgtcc
     5701 gggaaacccc ctccccggtc gggatcaccc cccggactct cccggaggtc atgcttgacg
     5761 ctctccagcg tatgggcggc ggctccaggg ggatcggggt cgacgatgcg gtcgcggccg
     5821 gggtgcgttc cggcctcacc gccggggagg cacgcgccgc gcttgaagag cttcttgccg
     5881 atggagagtg ctaccagccc gtggatggca caataaaact gatctgagac gattaatgcg
     5941 cctacatttc attgagaacg gccccgccct cctggtcgag caggagcagc gcgtcctggt
     6001 cgtcgccgac ctccacatgg ggatcgagtc cggtctcgaa cgctgcggag ttcatatcac
     6061 gagccggagc gacgcccgtg ccaaacgcgt gatcgcctgc atcgaggaga cccgacccga
     6121 tctcctgctc ctcctcggcg atctcaagca caacgtcccg gtgacaaccc ggcaggagta
     6181 ccgcgaactt ccgcgcatac tccagtcgtt tcgggaactc gtccctatcg ccgtcacccc
     6241 gggcaaccat gacggcggga tcgaccggtt cctccaggag ggcgaactcc tgcccgcggg
     6301 aggcgcgctc atcgacggcg tcgggtatct ccacggccac acccatcctg ccccctctct
     6361 tctcggccgc ctcataatcc ttggccacca ccacccggtc gtctcgctca tggactccgt
     6421 cggctgcgcc gcccgcgctc agccggccta cctctactcg gtatttgagg agccaacagg
     6481 ggagcgcagc cggctcctgt ttgtcccggc cttcaacgag ctcgcgggcg ggatcgatgt
     6541 cgggctgctg cgggagagcg ggcttggccc cctctcgcgc tgcatagacg ataagaacgc
     6601 ggaggtgttc ctggcagatg gcacgtacgt cggctccccg gccacgctct gccccccaga
     6661 cgccgacggc tgaactcctc gacccgcggg tgcgggaggt cgtccggaag cgcgggttca
     6721 cagagttttc tgaggcgcag gagcagtcca taccgcggct tctcgcgggg gacaacctga
     6781 tcctggtggc gccgacgggc accgggaaga ccgagagcgc gatgctcccc gtattcgacc
     6841 ggctcctcga gacgccgggt cccgggttca aggccctcta cataaccccg ctgcgctccc
     6901 tgaaccgcga tatcctggcg cggatggagt ggtggtgccg ggagctctcg ctcacggtcg
     6961 gcgtccgcca cggggacaca ccgcagaacg agcggcgtaa acaggcgctg aacgcccctg
     7021 acctgctcat aaccacgccg gagtcgctgc aggcgctctt catgggcaaa cgcctccgcg
     7081 aacacctcaa gaacgtcagg tatgtcataa tcgacgagat ccacgacctc gccgagagca
     7141 aacggggggc gcagcttgcg gtggctctcg aacgtctggc cgagtatgcc ggggagttcc
     7201 agaggatcgg cctctcggcg accgtgggga acccggacga gatcggccgg ttcctctgcg
     7261 gcgcacggcc gttctcggtc gtggaggttc cggtcgcaag caacctggat ataaccgtcc
     7321 ggtttgccgg cgaggacttc ggagaccagg tcaggacggt tgagaggtgc ctggacgccc
     7381 cgggctccac cctggtcttt gtcaacacac gcgtgaccgc ggaggccctc ggccacagcc
     7441 tctactccag gggggatgtc gaggttcacc acggttcgct ctcgcgggat gtccgggtgg
     7501 acgccgagga gcggttcagg aatggggaga tccgggcgct gatcgcgacc tcctcgatgg
     7561 agcttggcat cgatatcggg catatcgagc acgtcctcca gtttggctcc ccacgggagg
     7621 tgtcgcgcct ggtgcagcgg gtcggccgcg ccggccaccg tctcgacgcg gtatcgcgcg
     7681 gaaccgttct tgcgaccggg tttgacgacc tcctggagtc gcttgttata gcgcgccggg
     7741 cgaaggctaa cgagtgcgag agggtcgtcc cgcccgccgg cgccgccgat gtgcttgcaa
     7801 accaggtggc ggcgatcgcg gtcgagtacg gcgagatcga gatctcccgt ctccgaaaga
     7861 ttgtggagcg gtcgagcatc tttgccggct gcgggtctct cctggacgac gtctgccgtc
     7921 agatggcgga gcacaggctg atccgggtcg aggggaccag ggtcatccgg acggggcgtg
     7981 caaggcgcta ccttgtcggg aacctctcta tgatccatga cgagcggaag atggaggtct
     8041 ttgatatagt gtcgcgcagg acggtcggaa ccctggacga gtcgtttgtc ctgggctgga
     8101 tgcagaccgg cgcggtcttt atcacgaagg gccagctctg gcgggtgctc gatatggagg
     8161 gcgggaggat cacggtcgaa ccggccgcga gggtgagggg ggaggtgccc tcctgggagg
     8221 gggagcagat accggtgccg tttgcggtcg cccgcgaggt cggggcgctg cggcggatgc
     8281 gggcgttcgg ccgctactcg gacgttccgg gggtgatcgg gtatgcggag cggttcatgg
     8341 cccggatgga cgcaggaaac catcctgtcc catccgatcg gctcatcact ctggagaacg
     8401 ctgacgaggg cgtggtctgc aacgtctgcg gcggccataa ggcgaacgag acgctggcac
     8461 gggctctctc gatacttatt tcggcccgct acgggacgac cgtcgggatc gaggtcggcg
     8521 cgtaccggtt cctgctccgc ctgcccggga acgtccgggc gcttgaggtg caggaggcga
     8581 tggcggggat cgagcccgag caccttgccg ggatactgag acttgccctg aagcggacgg
     8641 cgctcttcaa gtggaaactt gtgcaggtgg ccaagaagtt cggcgccatc gatgcggacg
     8701 ccgactacga gcggttcagc atccatcgtc tgatcgacct cttcgacggg acggtcatag
     8761 cggcggaggc ctaccgggaa ctcttcagca gatacatgga tgtggaggcg gcagaggaga
     8821 tcctggccgg catacgtgac ggccggattg cggtcaccac aggccgtctc agcccgatcg
     8881 ggagcgaggg gctctcgtcg tcccgggata ctgtcccgcc gccgatgatc gaccaggcgg
     8941 tgatcgggac gctcatgcgc agactggaga aggaggaggt cgtcctcttc tgcatgaact
     9001 gccgtaagtg gaagagccgg acggtcgttg agcgcgtccc cgaccggccg cagtgccccc
     9061 tctgcggcgc tcgcctgatc gccgcgctga agccctggga cgaggaactc atcccggcgg
     9121 tgacgaagaa gaagaaatcg gaggaggagc gggcgatcga ggtccggttc ctccggaacg
     9181 ctaacattgt tctctcgagc gggaagaaag cggtgaccgc actcgcggca aagggtgtcg
     9241 gcccggagac ggcgtcgcgg atcctggcaa ccctctctga gggggatgcc ttttaccgtg
     9301 agatcctgaa ggctgagcgc aactacgtta agactcac
//
LOCUS       DAOSZX010000052         9303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6300_length_9303_cov_18.811743, whole genome shotgun sequence.
ACCESSION   DAOSZX010000052 DAOSZX010000000
VERSION     DAOSZX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6300_length_9303_cov_18.811743"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1812
                     /locus_tag="PK196_05470"
     CDS             454..1812
                     /locus_tag="PK196_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HON40901.1"
                     /translation="MQTKILLDEGEMPKRWYNIQADLPTPMDPPLHPGTGKPATPDDL
                     RHIFPMELIRQEMSTERYIDIPGEVRDILTLWRPSPLYRARRLEAALKTPAKIYYKWE
                     GVSPPGSHKPNTAIAQAYYNREEGIERLATETGAGQWGSALAFATSLFDMDCTVYMVR
                     ASYDQKPYRKSMMQVYGAECIPSPSDRTQAGRAVLAHDPDTPGSLGIAISEAVEDAAN
                     HDNTNYSLGSVLNHVCLHQTVIGQEAEEQLAIADDYPDVVIGCVGGGSNYAGLSFPFA
                     GAKLTGKHPDTELIAVEPAACPTLTKGLYTYDFGDVAGLTPLLRMFTLGHDFIPPAIH
                     AGGLRYHGASPLVSRLVHDGVIRAVAYHQNEVFEAALTFARTEGIIVAPEAAHAVKAA
                     IDQALLCRKTGEEKVILFNNSGHGNFDFSAYEAYLAGKLPDYEYPLELIKESLSRLPV
                     RG"
     gene            1865..3313
                     /locus_tag="PK196_05475"
     CDS             1865..3313
                     /locus_tag="PK196_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HON40902.1"
                     /translation="MEAATDRPLVVPVFLDVPLPPSSPADIYASLRDGPGFLLESLEG
                     SEKIARYSFICTAPTAMIAVAPDGALTVSGDPCIREVAAGIEAEDPVDAVRQFMERFR
                     VVPSTLPRFSGGLAGYFSYDLVSSIYPVRSVEETGDPVARFMLAQGCLALDHRRGRLA
                     VIENLLLTDESDLEEEYLRALTAIKNRITGLCHLPSSSSVEAFSAGAVTSSCTREEFS
                     NAVLRIKEHIAAGDIFQAVLSRRLACRFEGEPFGVYRRLREKNPAPYTYFLDFGDLVV
                     AGSSPEMLLRVEDGRVTTVPIAGTRPRGRTAAEDDQLAAELLADEKELAEHVMLVDLA
                     RNDIGAVSAYGSVSVTDFMGIERFSHVQHIVSTVSGRLREGYDRFDALRSCFPAGTVS
                     GAPKVRAMQIIGEVEGLRRGIYAGALGHIAFSGTMDLAITIRTVIVRDGVASVQVGAG
                     IVADSDPGREFAETGSKARAMLAALGREEEVD"
     gene            3313..3894
                     /locus_tag="PK196_05480"
     CDS             3313..3894
                     /locus_tag="PK196_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HON40903.1"
                     /translation="MRVLVIDAYDSFTFNLCQQIGVLGAEPVVVKSDTPFARIRSLRF
                     DRVVLSPGPGHPGECALYREVIETVSRTVPTLGVCLGHQAIGLAFGADVVRAERLMHG
                     KRSVIHHDGAGIYEGVPDPIIATRYHSLLIDPETVPDCLEVTARSDDDGAIMGVRHRE
                     FPIEGVQFHPESILTPDGDRLIANFLSGRGGAA"
     gene            3891..4907
                     /gene="trpD"
                     /locus_tag="PK196_05485"
     CDS             3891..4907
                     /gene="trpD"
                     /locus_tag="PK196_05485"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HON40904.1"
                     /translation="MIREAIARVSSGSDLTAVEAEGVMEEIMRGAATPAQIGGILTAL
                     RMKGEAVAEIAAFARVMRAAAVPVPLPHAGMRVDTCGTGGDGAGTFNISTTAAFVAAG
                     AGVPIVKHGNRGVSSRCGSADVLEALGVAVAIPPERVAAVLSAAGIAFLFAPAYHPAM
                     QYARAARQEIGIRTVFNLLGPLTNPAGAAGQLLGVYDPHLVVPLARVLGDLGVQRAMV
                     VHGAGLDEITTTGETTVAELMEGEVVSYTLDCTKFGIPRSPPAAIRGGGPEENARILL
                     SVLAGEDGPARDIVLLNAGAAIYIGGKADDLAGGIECAEASIDSGAALDRLHRLARVS
                     GGGA"
     gene            4904..5662
                     /locus_tag="PK196_05490"
     CDS             4904..5662
                     /locus_tag="PK196_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HON40905.1"
                     /translation="MILDDIVRAAGERLAGLDQTKAAGTGGMPHRSLEAAILSCRERH
                     AIIAEVKYASPSRGRIHDGATPAAIAREFADAGAVALSVLTEPTYFGGSLENLARVRG
                     AVSLPILRKDFIIDERQVAETRAFGADAVLLIARLLGDRLSAFVDAALEIGLEPLVEV
                     HNRGEMERALATGANLIGINNRNLETMTVDLSTTVRLARAARDAGRTVVSESGIAWPY
                     DVRRLSRHCDAFLIGSALMSAKDRQKRLEGFVFA"
     gene            5674..6231
                     /locus_tag="PK196_05495"
     CDS             5674..6231
                     /locus_tag="PK196_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HON40906.1"
                     /translation="MTNVRDALVAAEAGADAIGVVLASPSPRSVRPEEAREIFAAVPP
                     SVTTVAVTSTGRPEDLPAILSLRPDAVQVPADLEVPSRAGLRVIRMLAPGDPLRDDCD
                     AVIIDASHGRGRAFDPGYARRCVASSSVPVILAGGLNPWNVAAAVRAVRPYAVDVCSG
                     VEAMPGLKDGRLVRAFVKACRMMDP"
     gene            6228..7403
                     /gene="trpB"
                     /locus_tag="PK196_05500"
     CDS             6228..7403
                     /gene="trpB"
                     /locus_tag="PK196_05500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HON40907.1"
                     /translation="MKAGRYGIYGGQYVPETLMSALIELEETYTRVRDDPDFSGRLSW
                     YLHEYAGRETPLTFCENLSRDLGCRVYLKREDLLHGGAHKLNNTLGQGLMAKYMGKRR
                     LIAETGAGQHGVATAIAGAVLGLPVEVYMGEVDTRRQALNVFRMELLGARVIPVTSGT
                     RTLKDAINAAMRDWVANLRDTHYLLGSCVGPHPFPRIVRDFQSVIGEETRRQILEREG
                     SLPDMIVACVGGGSNAIGMFYPFVGDADVALVGVEAGGEGPESCRHGASLSRGSVGVF
                     QGSLSYLLQDDDGQTLETHSIAAGLDHPAVGPEHAMLKDSGRIRYEMATDTEALQAFR
                     CLSRTEGIIPALESAHAVAFALRAADELDRDGILVINLSGRGDKDVVEVAKHHGGLG"
     gene            7400..8206
                     /gene="trpA"
                     /locus_tag="PK196_05505"
     CDS             7400..8206
                     /gene="trpA"
                     /locus_tag="PK196_05505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HON40908.1"
                     /translation="MSRIERVFARGGPVFIGFTVAGDPDMERSFFAATAMIDAGADML
                     EIAVPYSDPVADGPVIERAHHRALRAGTRPVDVFLLLRRLRDYAPDLPLVLFTYYNIV
                     YRRGVDRFFSEAAAAGADGLLIVDLPVEESEEVAPHAARYGIDRIALIAPTTSPERQH
                     AILREASGFVYLISLEGVTGERDHLPPGLAGLVGAVREKTDLPLAVGFGVSRPEHVRT
                     VLDAGADGVIVGSALVRLIEEHMDDGPRLCEALRAAIVSLRDGFSSSSRT"
     gene            8237..>9303
                     /locus_tag="PK196_05510"
     CDS             8237..>9303
                     /locus_tag="PK196_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HON40909.1"
                     /translation="MRSIGFLVNPVAGMGGAVGLAGTDGKVDEAIRRGAVPRAADRAL
                     QALSPLRGEEIRWYTSAAPMGRDVLSSAGIERCTVLHHPAAPTTAADTRAACRAFLEA
                     GVDLILFCGGDGTARDVLDAVGREVPILGIPAGVKMYSAVFAVNPAAAADLIRRAGEI
                     PCRESEVMDVDEEAYRSGRLTTHLYGYARVPFIPERIQGGKQVFEQQDEERAKDDIAA
                     FISEVMLPETLYIIGAGSTTARIMERLGLVPTLLGVDVVRNGEILARNADERTLLALL
                     DKHPRAKVILSPIGAQGFVLGRGNQQISPAVLREAGLSNLIVVATPGKLAATPLLYVD
                     SGDPELDREVGDSLQVISGYR"
BASE COUNT         1567 a         2887 c         3115 g         1734 t
ORIGIN      
        1 ttcaccgccg tggttatgga gggcatcaca ccatgagcaa actggtttta tagttcccgt
       61 cgcgtaatac cgggaagaaa gggggacttt atatcacaga accgttgatt tgatggcctc
      121 atataaaccg ggtgatggcg gtcacagctt cccccgccag caggcaggca aaaatcaggt
      181 gattggggag agcagatcct gcattcgccc acgcacctcg cgaggatgat tatagtgggg
      241 tggtctgtcc ctttcaggcc gtggtcgatc gccgcgctaa cgatgctgat gggaggacca
      301 tcgtgttgtg ggtgaagaag gcagatcgct gcgcggtggt ggaatgggta taggccattc
      361 ctctgtcttc tgactggttt tccggataat cagatcatca tagatcaaca gtggataata
      421 atgtatatat gtgtgcaaag ttcatgttga tctatgcaga ccaagatcct ccttgacgag
      481 ggggagatgc caaagcggtg gtacaacatc caggcggatc tcccgacgcc aatggatccg
      541 ccccttcacc cggggacggg taaaccggcg acgccggatg acctccgcca catctttccc
      601 atggagctga tccggcagga gatgagcacg gagcgctaca tcgatatccc tggcgaggtc
      661 cgggatatcc tcaccctctg gaggccaagc cccctctacc gtgcaaggag gcttgaggcg
      721 gccttgaaga ccccggcgaa gatctactac aagtgggagg gtgtgagccc gccgggctcg
      781 cacaagccca ataccgccat agcccaggcc tattacaacc gggaggaagg gatcgagcgg
      841 cttgcaaccg agaccggggc ggggcagtgg ggttctgcac ttgcgttcgc aacgagcctc
      901 tttgatatgg attgcaccgt ctacatggtc cgggcatcat acgaccagaa accctaccgc
      961 aagagcatga tgcaggtcta cggtgccgag tgcatcccga gcccctctga taggacgcag
     1021 gccggcagag cggtgcttgc ccacgacccg gatacgccgg gctccctcgg tatcgccata
     1081 tccgaggcgg ttgaggacgc cgcgaaccac gataacacaa actactcgct tggttccgtc
     1141 ctcaatcatg tctgcctcca ccagacggtc atcggccagg aggcagaaga gcagcttgcg
     1201 attgcagacg attacccgga tgtggtgatc gggtgcgtcg gcgggggaag caactatgcg
     1261 gggctttctt tcccgtttgc gggcgcgaag ctgaccggaa agcacccgga taccgaactc
     1321 attgcggttg agcccgccgc ctgcccgacc ctgacaaagg ggctctacac ctacgatttc
     1381 ggggatgtcg cagggctgac accgctcctg cggatgttca ctctcgggca cgactttatc
     1441 ccgccggcga tccacgctgg agggctccgc tatcacgggg catctcccct ggtatcgagg
     1501 ctcgtccacg acggggtgat cagggcggtt gcctaccatc agaacgaggt ctttgaggca
     1561 gccttgacct tcgcccggac ggagggcatc attgtcgcgc ccgaggccgc ccacgcggtg
     1621 aaggccgcga tcgaccaggc gctcctctgc cgtaagaccg gtgaagagaa ggttatactc
     1681 tttaacaact ccgggcacgg taatttcgat ttctccgctt acgaggccta ccttgcggga
     1741 aagcttcccg actacgaata ccccctggag ttgatcaaag agtctctctc caggctgccg
     1801 gtgagggggt gatgccggtg gactggactg cgctcgaaga gagctgaccc caatccaggg
     1861 attcatggag gccgcgaccg accggcctct cgtagtcccg gtatttcttg atgttccact
     1921 ccctccctct tctccggcag atatctatgc gtccctccgg gacggccccg ggttcctgct
     1981 ggagtcgctc gaggggagcg agaagatcgc tcgctactct tttatctgca ccgctccaac
     2041 cgctatgatc gccgtcgccc cggacggggc gctgacggtc tccggcgatc cctgtatccg
     2101 cgaggttgcc gccggaatcg aggctgaaga cccggtcgat gccgtccgcc agttcatgga
     2161 acggttccgg gtcgtgccgt cgacgttgcc gcggttctcc ggcgggcttg cgggctactt
     2221 ctcctacgac ctggtctcgt ctatctaccc cgtccgctct gtggaggaga ccggagaccc
     2281 tgtcgcccgg ttcatgctgg cgcagggctg cctcgccctc gaccaccggc ggggtcgact
     2341 tgcggtgatc gagaacctcc tcctcaccga tgagtccgat ctggaggagg agtacctccg
     2401 cgccctgacg gctataaaga accggatcac ggggctctgc cacctccctt catcctcttc
     2461 agtcgaggcg ttcagtgccg gtgccgtgac gtcgtcctgc acccgggaag agttctcgaa
     2521 cgctgtttta aggatcaagg agcatatcgc ggcaggcgac atattccagg ccgttctatc
     2581 ccggcggctt gcctgccggt tcgagggcga accgttcggt gtctaccggc gactccggga
     2641 gaagaatccc gccccctaca cctacttcct ggacttcggc gacctggtgg ttgccgggag
     2701 cagtcccgag atgctgctgc gggtggagga cggccgtgtg acgaccgtcc ccatcgccgg
     2761 cacccggccc cggggacgga cagcggcgga ggacgaccag ctcgcggccg aactcctggc
     2821 ggacgagaag gaacttgcag agcatgtcat gctcgtcgac ctggcgagga acgatatcgg
     2881 ggcggtatcg gcctatggga gcgtctcggt gaccgacttc atgggtatcg aacggttctc
     2941 gcacgttcag cacatcgtct cgaccgtctc tgggaggctc cgggagggct acgaccggtt
     3001 cgacgcgctc cggtcatgtt tcccggcggg aaccgtctcc ggcgccccga aggtcagggc
     3061 gatgcagatc ataggcgagg tggagggtct ccggcgcggg atctacgcag gtgccctcgg
     3121 gcatatcgcg ttctccggca cgatggacct cgcgataacc atccggacgg tcatcgtgcg
     3181 ggacggtgtc gcctccgtcc aggttggggc gggtattgtg gctgattccg accccggtcg
     3241 cgagtttgcc gagaccggca gcaaggccag ggcgatgctt gcggcgctcg gcagagagga
     3301 ggaggtggac tgatgcgggt gcttgtgatc gatgcctacg acagtttcac tttcaacctc
     3361 tgtcagcaga tcggggtgct cggggccgaa ccggtcgtgg tgaagagcga taccccgttt
     3421 gcgcggatca ggtcgctgag attcgaccgc gtcgtcctct cgccgggacc cggccacccg
     3481 ggcgagtgtg ccctctaccg ggaggtcatc gagactgtca gcaggacggt cccgacgcta
     3541 ggggtctgtc tcggccacca ggcgataggg cttgcattcg gcgcggacgt ggtccgggca
     3601 gagcggttga tgcacggaaa gcggtcggtc atccaccacg acggtgccgg tatctacgaa
     3661 ggggtgcccg acccgatcat cgctacccgt taccactcgc tcctcatcga ccccgagacc
     3721 gtccccgact gcctcgaagt gactgccaga agcgacgacg atggggcgat catgggcgtc
     3781 cggcaccgtg agttcccgat cgagggggtg cagttccacc cggagagtat cctcaccccc
     3841 gatggggaca ggctgattgc aaacttcctc tccggcagag gcggtgcagc atgatccgcg
     3901 aggcgatagc ccgtgtatcc tcgggcagtg acctcaccgc tgtcgaggcg gaaggggtga
     3961 tggaggagat catgcgcggc gccgcgactc ctgcccagat cggcgggatc ctgacagcgc
     4021 taaggatgaa aggggaggcc gttgcggaga tcgcagcatt tgcccgggtg atgcgggcgg
     4081 cggccgtccc tgtccctctc ccccacgcag ggatgcgggt ggatacctgc gggacggggg
     4141 gcgacggcgc ggggacgttt aacataagca ccacggccgc ttttgtggcg gcaggggcgg
     4201 gcgttcccat cgtgaagcac gggaaccgtg gggtgtcgag caggtgcggt tcggccgacg
     4261 ttctcgaggc gctcggcgtc gctgtcgcga tcccgcccga gcgggtggcg gccgtcctct
     4321 ctgctgccgg gatcgccttc ctcttcgccc ccgcctacca ccctgcgatg cagtatgcca
     4381 gggcggcccg ccaggagatc gggatcagga cggtattcaa cctccttggc ccgctcacca
     4441 acccggcggg ggcggcaggt cagctcctgg gggtctatga tcctcatctt gtcgtcccgc
     4501 ttgcccgggt tctcggcgat ctcggggttc agcgggcaat ggtggtccac ggtgcagggc
     4561 tcgatgagat caccacgacc ggggagacga cggtcgcaga actcatggag ggggaggtgg
     4621 tctcgtatac gctagactgc acaaaattcg gtatcccgcg ttcgccgcct gccgccatca
     4681 ggggtggcgg cccggaggag aacgccagga tcctcctctc ggtcctcgcc ggtgaagacg
     4741 gcccggcgcg ggatatcgtc ctcctcaacg ccggggcggc gatctacatc ggtgggaagg
     4801 ccgacgacct tgccggaggc atcgagtgcg cggaggcgtc gatcgactca ggggcggcgc
     4861 ttgaccggct ccacaggctt gccagggtga gcgggggcgg ggcatgatac tcgatgatat
     4921 cgtccgtgcg gcaggtgagc ggctggcggg tcttgaccag acgaaggccg ccgggaccgg
     4981 cggaatgcct caccggagcc tcgaagcggc cattctttcc tgccgggagc ggcacgcgat
     5041 catcgccgag gtgaagtacg cctccccctc ccgcggcagg atccatgacg gtgccacacc
     5101 ggcggcgatt gcccgggagt tcgccgatgc cggggctgta gcgctctcgg tgctcaccga
     5161 acccacctac ttcgggggga gtctcgagaa ccttgcccgg gtgcgtggtg cggtctccct
     5221 ccccatcctt cgcaaggact tcatcatcga cgaacgccag gtcgctgaga cccgggcgtt
     5281 cggcgccgac gctgtcctcc tgatagcccg tctgctcggc gatcggctct ccgcgttcgt
     5341 cgatgcggcg cttgagatcg ggcttgagcc gcttgttgag gttcacaacc ggggcgagat
     5401 ggagcgtgct ctcgcgacag gtgcaaacct gatcgggatc aacaaccgca acctggagac
     5461 gatgacggtc gatctctcga cgaccgtccg cctggcgcgg gcggcccggg atgccgggag
     5521 gaccgtggtc tctgagagcg ggatcgcctg gccctacgat gtcaggcgtc tatcgcggca
     5581 ctgtgacgcc ttcctgatcg gttcggcact gatgtcggca aaagatcggc agaaacggct
     5641 ggaggggttt gtattcgcgt aaagatctgc gggatgacga acgttcgcga cgcacttgtc
     5701 gccgctgagg ccggtgccga cgcgataggt gtggtgcttg cgagcccctc ccccaggtct
     5761 gtgaggccgg aagaggcgcg tgagatattt gcggcggtcc cgccgtctgt gaccaccgtc
     5821 gccgttacat cgaccggaag accggaagac ctcccggcca tcctctcctt acggccggac
     5881 gcggtccagg tgccggctga cctggaggtg ccctcccgtg cgggtctccg ggtcatccgg
     5941 atgcttgctc ccggcgaccc tctgcgggac gactgtgacg ctgttataat cgatgcaagc
     6001 catggcagag ggcgagcgtt cgaccccggt tatgcccggc gttgcgtggc ctcctccagc
     6061 gtccctgtaa tcctggcggg cggcctgaac ccctggaacg tggccgcggc cgtccgggcg
     6121 gtgcggccgt atgccgtcga tgtttgttca ggtgtcgagg cgatgccggg tctgaaggac
     6181 gggcggcttg tgcgtgcgtt tgtgaaggca tgcaggatga tggatccatg aaagcaggac
     6241 gatacggtat atacggcggg cagtacgtgc ccgagacgct gatgagtgcg ctgatcgaac
     6301 ttgaggagac ctacaccagg gtgcgggatg acccggattt ctccggccga ctctcctggt
     6361 atcttcacga gtacgccggc cgggagacac cgctcacatt ctgcgagaac ctctcccgtg
     6421 acctgggttg ccgggtctac ctgaaacggg aggacctcct ccatgggggt gcgcacaaac
     6481 tgaacaacac cctgggtcag gggctcatgg caaagtacat ggggaagaga cggctcatcg
     6541 ccgagaccgg cgccgggcag cacggtgtcg cgaccgcgat cgcgggggca gtccttgggc
     6601 ttcccgtgga ggtctacatg ggggaggtgg ataccaggcg ccaggcgcta aacgtctttc
     6661 ggatggaact ccttggcgcc agggtcatcc ctgtcacctc ggggacgcgg acgctcaaag
     6721 acgccatcaa cgcggcgatg cgggactggg tggcaaacct ccgggacacc cactatctgc
     6781 tcggctcctg tgtcggcccg cacccattcc cccggatcgt cagggacttc cagtcggtca
     6841 tcggcgagga gacgcggcgg cagatccttg agcgggaggg aagcctcccg gacatgatag
     6901 ttgcctgtgt cggcggtgga tcgaacgcaa tcggtatgtt ctacccgttt gtgggtgatg
     6961 ccgacgtggc tctcgtgggc gtggaggccg gcggcgaggg gccggagtcc tgcaggcacg
     7021 gcgcttctct ctcgcggggg tcggtcgggg tcttccaggg ctctctctcc tacctgctac
     7081 aggacgacga cggccagacc cttgagacgc actcgatcgc cgccgggctc gaccatcctg
     7141 ccgtcggccc ggaacacgcc atgctgaagg attccggccg gatcaggtat gagatggcca
     7201 ccgataccga agcgctccag gccttccgct gcctctcccg cacggagggc atcatcccgg
     7261 cgctcgagtc ggcccacgcc gtcgcctttg ccctccgcgc ggcggatgaa cttgaccgtg
     7321 acgggatcct ggtaatcaac ctctcgggga ggggcgacaa ggatgtcgtc gaggtcgcga
     7381 agcatcacgg aggtctggga tgagccggat cgagagggtc tttgccaggg gcggcccggt
     7441 cttcatcggg tttaccgttg ccggtgaccc tgatatggag agatcgttct ttgcggcaac
     7501 agccatgatc gacgcgggag cggatatgct tgagatcgct gtaccttact ccgaccccgt
     7561 tgccgacggc ccggtgatcg agcgggcgca ccaccgcgct ctgcgggccg ggaccagacc
     7621 tgtagatgtc tttctccttc tcaggaggct ccgggattat gcacccgatc tccccctcgt
     7681 cctcttcacc tactacaaca tcgtctaccg gcgtggagtc gaccggttct tttccgaggc
     7741 cgcggccgcc ggcgcggacg gtctcctaat agtcgacctt cccgtcgagg agtcagagga
     7801 ggtcgcgcct cacgccgcca gatacggcat cgaccggatc gccctcatcg ccccaacgac
     7861 ctcgccggag cggcagcatg caatcctccg ggaggcatcg gggtttgtct acctcatctc
     7921 gcttgagggg gtgacgggcg agcgcgacca cctcccgccc ggtctcgccg ggctggtcgg
     7981 cgcggtgcgg gagaagaccg atctcccgct cgccgtcggg ttcggggtct cgcgcccgga
     8041 gcatgtcagg acggttctgg atgcgggggc ggacggcgtc atcgtgggga gtgcgctcgt
     8101 caggctgatc gaagagcata tggacgatgg gccccggttg tgcgaggcgc tccgggcggc
     8161 tatagtctcc ctgcgggatg ggttttcatc ctcatcccga acctgatatg ccctgacgcg
     8221 aaagactaca tggatgatgc ggagtatagg tttcctggta aacccggttg ccgggatggg
     8281 cggggcggtt ggccttgcgg ggacggacgg caaggtcgat gaggcgatcc ggcggggtgc
     8341 ggttccgcgt gctgctgatc gggcgctcca ggccctctcc ccactccgcg gcgaggagat
     8401 ccggtggtac acgtctgcgg cgccgatggg ccgggatgtc ctgtcgtcgg cggggatcga
     8461 gcgctgcacc gtcctccacc accccgccgc cccgaccact gccgccgata caagggcggc
     8521 ctgccgggcg tttcttgagg ccggggtcga cctgatcctc ttctgcggcg gggacgggac
     8581 ggcccgggac gtcctggatg ccgtgggccg ggaggttccg atcctgggga tcccggccgg
     8641 tgtgaagatg tactcggcgg tctttgcggt caacccggcg gccgcggccg acctcatccg
     8701 ccgggcgggc gagatcccct gccgcgaatc cgaggtgatg gacgtcgatg aggaggccta
     8761 ccgctcaggc cgtctcacca cccatctcta cggctacgcc cgcgtcccct tcatcccgga
     8821 gcggatccag ggcgggaaac aggtctttga gcagcaggat gaggagcggg caaaggacga
     8881 tattgcggcg tttataagcg aagtgatgct ccctgagacg ctctacatca tcggcgccgg
     8941 gagcacgacg gcgcgtatca tggagcggct cggtcttgtc ccgacgctgc tcggggtgga
     9001 cgttgtccga aacggcgaga tcctcgccag gaacgccgat gagcggactc ttcttgccct
     9061 tctggacaaa cacccgcggg cgaaggtcat cctcagcccc atcggtgccc aggggttcgt
     9121 ccttggccgg ggaaatcagc agatcagccc ggccgtcctc cgggaggccg ggttatcgaa
     9181 cctgatcgtg gtagccacac ctggaaagct tgccgcaacg cctcttctat acgtcgattc
     9241 cggggacccg gagcttgacc gtgaggtcgg ggactctctc caggtcatct ccggctaccg
     9301 gat
//
LOCUS       DAOSZX010000053         9286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6324_length_9286_cov_16.219045, whole genome shotgun sequence.
ACCESSION   DAOSZX010000053 DAOSZX010000000
VERSION     DAOSZX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_9286_cov_16.219045"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..746
                     /locus_tag="PK196_05515"
     CDS             354..746
                     /locus_tag="PK196_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40910.1"
                     /translation="MKMKLIGKIFPALTLALMCGALVSLVGAWEIDPINEPIAANISV
                     DDGMHPLWTGYPYPGIGDADVHNDLAKVAGLKMGLPVEYYSWLSKLAGQPDDPNFDDY
                     TLHPSETHYGSHSSFHEERPGGCRVLCE"
     gene            706..1146
                     /locus_tag="PK196_05520"
     CDS             706..1146
                     /locus_tag="PK196_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40911.1"
                     /translation="MKNAPEVAEYYANKARDYIRNGDPNNGYIQLAYAMHFMSDMGCP
                     YHYTYEGLANHPKYEGFVGDNWHTGHYFYRDILDADYYYSISDVSDAANNLANAAHQY
                     QSYFDSQIWHNSDWKIDPKLIEDTRTVLIYTERYDRGLVDYVNR"
     gene            1180..2070
                     /locus_tag="PK196_05525"
     CDS             1180..2070
                     /locus_tag="PK196_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40912.1"
                     /translation="MTKQPSQRTLTALLGLAVFLTISVPCSLLIVLGTGCPGALAAAL
                     TLLLGGILMLLILQRYRKYRYRPGPALLAVVIGCAGALLAVLGVLIFGLAWADVNPAT
                     YTYRVTVTGLDNYTGGPVTDILVPLPLREGKMIFSEEDLEGQRVGAWKTLFAETPKGR
                     MLVFQTMERDLSDISVTFGSPVEKYFLIDDITRESFSPVVTEAPAGYTRWAFGTGDVQ
                     NFTTLVRLDEGIRPKTADAGPIVIHLWITAGGGRLHGITGEKYQVEVYEAIPPGVTGF
                     VPVRAQVARFGDVGIVPVRE"
     gene            2237..3028
                     /locus_tag="PK196_05530"
     CDS             2237..3028
                     /locus_tag="PK196_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40913.1"
                     /translation="MKTPETRTITLLIAILLPLAYLTYWFLRMSSLPLIADVVLAAGV
                     IAAVALGIIARRRGAAVRWLTASFVVGLVLCGLIFLGMGIWAGWYEHRPGLVGYQVWV
                     DGLEGRTGGLITTILVPLPVEDGEVIMPASEFENRTFDGWTTTIVETEDGKMLAFQNR
                     NNTLTDIWAHFTRYEETIEGTRRLPKEHLSPVIERTTDDHYTTVIFVDEGISPPGELR
                     VTLRLTAGCDLFHGMAEDTYRTEVKETIPAGTAGRIVVDAMVGKY"
     gene            complement(3459..4037)
                     /locus_tag="PK196_05535"
     CDS             complement(3459..4037)
                     /locus_tag="PK196_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HON40914.1"
                     /translation="MSMLRLAPDCPSPYCDDVRMIRAFIRDTDEDGHRAEIPIGWWCP
                     RCGWLVNDITGEKIIAIHDTLIALHDRPEDRGWEGLKDPGCVETWVYLWNNYWVCQQN
                     IYRKAAWFLEVVTNGHGFWQGNKRTAYTATDVLILRANGYIFDADAEEVDRFMVTLAD
                     LDDDGNSRYTLDEVEEWIRANTKRIDLFSVLA"
     gene            complement(4077..4433)
                     /locus_tag="PK196_05540"
     CDS             complement(4077..4433)
                     /locus_tag="PK196_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HON40915.1"
                     /translation="MTYRVVITARPVTTSRSIPRPIAVRIGEELAALAGEADPKRHLK
                     KLKGSDNPAFYSLRVGDYRTILSVIDDLLVIHVVAVGPSGVLTSPGTPSPPFRVRATI
                     GSPGEPILLSTKPTRD"
     gene            complement(4430..4667)
                     /locus_tag="PK196_05545"
                     /pseudo
     CDS             complement(4430..4667)
                     /locus_tag="PK196_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4859..5326)
                     /locus_tag="PK196_05550"
     CDS             complement(4859..5326)
                     /locus_tag="PK196_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HON40916.1"
                     /translation="MTGASRGRLRYHLDMLIREGKVAAVDYRNRFRHFARNQRHTALE
                     KRIIASLRDETPGAILAHLLRLPGATRNEIAERLGLSGPTVSWQMQQFEDEGIVTVDR
                     EERTFCYRLSTGALEFLNRYMGDLPVTGHPGAGGDLPARGHDVLSGLDMGMFS"
     gene            complement(5556..6146)
                     /locus_tag="PK196_05555"
     CDS             complement(5556..6146)
                     /locus_tag="PK196_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40917.1"
                     /translation="MKQSHVGGIALLVCLIVVVAIALVSCAQDGTGSPAVGDNTPPLV
                     IGDTMTVLVTGAASLSDLAAAGIPAPEELQIPKGIKRYDVVAFDHAALSREIRDGFPL
                     SIHGMAYRAEQHGMNFEQIDDGINTYEGVIVGVDESDILLTIGNNVLVGSIVFGNETF
                     WIIPVEPRARAEQSASPLHVIYSSKDVPAGPLVPID"
     gene            complement(6382..8760)
                     /gene="lon"
                     /locus_tag="PK196_05560"
     CDS             complement(6382..8760)
                     /gene="lon"
                     /locus_tag="PK196_05560"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HON40918.1"
                     /translation="MYSKDTSNEHIVIPLSEVVVYPETRTKFLVGKTVGEALIAATKS
                     GSSASAVGLTAKSDAEAVENPVDAFYTVGTLLSIMRMQPADEGYIVFAHATSRVRAVA
                     LSEIEGVYHASCEPLPDIDDLDEEGREEMLRDVRAAIHEISSNFQGSEQFTRPVDGME
                     SVDQIMAFVMPFMPVDVATKQSLLETVSVKERYNAFLSLLVDFNERLNLRIEVARKAT
                     EKVGKANREALLREQLRVIQEELSEGEGSTGDDDYRERIERSAMPDEVRKKALSELKK
                     LEMGGSQHHESQGIRNYLDLLLDLPWTVGETKEIDIVEARRVLDSNHNGLEKVKERIV
                     QHLAVMKLKQEKQGSILLLVGPPGTGKTSLGRSIAEALGRKYVRISLGGVRDEAEIRG
                     HRRTYVGALPGRIIQGIRRAGTKNPVFVLDEIDKVGSSYLGDPASALLEVLDPEQNST
                     FSDHYLEVPYDLSDVFFITTANTLSTIPAPLLDRMEVIEISGYTKNEKFAIAKDHLLP
                     ETLAEHGLDEEQLQIDDGAIEAIIDRYTREAGVRGLKKQLARIARFVSTKIVSGTADL
                     PIVVTADMLPEILGRETVRQDVARKENPPGVVTGLAWTPVGGDILFIEGTFMPGKGKL
                     TLTGQLGDVMKESAQISLSLIRSRLAGMPSRFDFFSSDIHIHVPSGATPKDGPSAGVT
                     IFTALASLVTGRAVDPTIAMTGEVTLSGAVLPVGGIKEKVLAAHRAGIKTVILPRENE
                     RDLEDVPEEVRRELAFVTADTIEDVLRETLGLDLAGPVMPYAERRCVPVHDL"
     gene            complement(8819..9238)
                     /locus_tag="PK196_05565"
     CDS             complement(8819..9238)
                     /locus_tag="PK196_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HON40919.1"
                     /translation="MSARGERLFEVFERLFAIRDECSCEILSECGLGDVTAKQISYLK
                     VIDRHGDVTFSRLAEITRNSKPTVTEMVNRFVRMECAYRQKSPDDGRVTYIRLTEKGR
                     MLANADRKALQRMVERMVQALDEKEVDLLVEVLRKVG"
BASE COUNT         1857 a         2766 c         2664 g         1999 t
ORIGIN      
        1 atcattcctc gtgacgcccg gccccgagtg cgcaccctgc tgcacaccag tctttggggg
       61 aagcaccgga gtatcaggtt ccgatacctg cactcccgct cttttccctg aacttgaggt
      121 ctctgagcgc ccggatgaat cggcgttcgt ccgagaaacg gtataaagaa ggacagcgaa
      181 gtctcgatcg gggaagccgc acgcggtatc ccgagggggc cgggtgtctc gattggcatc
      241 gtgccccgac ccccacagcg tgcccggagg gcaatagggc tgttggctgt catcggggat
      301 agccgttgtg gttctgcggt ctcccagagc acgcatgaat gaggtgaaaa ccaatgaaga
      361 tgaaattgat tggaaagata tttcccgctc ttacgttagc actcatgtgc ggtgctctcg
      421 tttcgcttgt cggggcatgg gagatagacc ctattaatga accgattgct gcgaatatct
      481 cggtcgatga tggtatgcat cctttgtgga cgggataccc atacccaggt attggggatg
      541 ctgacgttca taacgacttg gcaaaggttg cagggctaaa gatgggactt ccagttgagt
      601 actacagttg gctgagtaaa ctggcaggac aaccagatga tcctaacttc gatgactata
      661 cgctccatcc gagtgaaacg cactatggct cacactcttc ctttcatgaa gaacgccccg
      721 gaggttgcag agtactatgc gaataaagca agggactaca tcagaaatgg tgaccctaac
      781 aacgggtata ttcagttggc gtatgccatg catttcatgt cggatatggg ctgcccgtat
      841 cattacacct acgaaggtct agccaatcat ccaaaatatg aaggatttgt cggggacaat
      901 tggcataccg ggcattattt ttacagagat atcttagatg ccgattatta ttactcgata
      961 tctgatgtaa gcgatgctgc aaacaatctc gcaaacgctg cacatcagta ccagagttat
     1021 tttgatagtc agatctggca caactcagac tggaaaatcg atccgaaact cattgaagat
     1081 acaaggactg tccttatata taccgaaagg tacgatcgag gccttgtcga ttatgtaaac
     1141 agatagatat cttcccattt tctccggaaa ctgattgtca tgacaaaaca acccagccaa
     1201 agaaccctta ccgccctcct gggcctggcc gtcttcctta ccatctcggt cccgtgcagt
     1261 ctcctcatcg ttctcggaac cgggtgtcct ggtgccctgg cagcagcgct caccctcctc
     1321 ctgggaggga ttctcatgct cctgatcctc cagagatacc ggaagtacag gtatcggccg
     1381 ggccccgctc tgctcgcggt cgtcatcggg tgtgccggtg cgctcctggc agtcctcggc
     1441 gttcttatct tcggccttgc ctgggctgat gtcaacccag ccacctacac ctaccgggtc
     1501 acggtcaccg gactcgataa ctataccggc ggtcctgtca ccgacatcct ggttccactc
     1561 ccgcttcgtg agggaaagat gatcttctcc gaagaagacc ttgaagggca gcgcgttggc
     1621 gcctggaaga ctctctttgc agagacaccg aagggaagga tgcttgtctt ccagaccatg
     1681 gagcgcgatc tctcggatat cagcgtgacc tttgggagtc cggtggagaa gtacttcctc
     1741 atcgatgata taaccagaga atccttcagc ccggtcgtca ccgaggcacc ggccgggtat
     1801 accaggtggg cctttgggac cggggatgtt cagaacttta ccaccctggt caggctcgat
     1861 gaggggatca ggcctaagac tgctgatgcc gggccgatcg tcatccacct ctggattaca
     1921 gcaggtggcg gacgtctcca tggcatcacc ggggagaagt accaggttga ggtttatgag
     1981 gcgattcctc ccggtgtcac cggctttgtc ccggtgaggg cccaggtggc caggttcggg
     2041 gatgtgggga tcgtaccggt gagagaatga ggtgagatgt gtgcactaca gccaaccatc
     2101 gcagattggg gagagggggt ggggagagtt caacgcgctg gagggaaaca ttatggggcg
     2161 caacgccgag gtctcaaccg ggaaagccac gtgagcccgg gacctcccgc tcagcctcgc
     2221 acaccctcat gatcccatga agacccctga aacccgtacc attaccctgc tcatcgccat
     2281 cctcctcccg ctggcctacc tgacctactg gttcctccgc atgagttcgc tccccctcat
     2341 cgctgacgta gtgctcgccg ctggtgtcat cgccgccgtg gccctgggaa tcattgcgcg
     2401 tcggagggga gcggcggtcc gctggctcac ggcctcgttc gtcgtcggtc tcgttctctg
     2461 cggcctgatc ttcctcggca tgggcatctg ggccgggtgg tatgagcacc gtcccggcct
     2521 tgtcggttac caggtctggg tagacggtct tgaaggccgg accggagggc tcataacaac
     2581 gatcctcgtc cccctcccgg tggaggatgg cgaggtgatc atgccggcgt ccgagtttga
     2641 gaaccggacg ttcgatggct ggacaacgac catcgtcgag acagaggatg gaaagatgct
     2701 tgcgttccag aaccggaaca acaccctgac cgacatctgg gctcacttca cgcgctacga
     2761 ggagaccata gaagggacaa ggcgcctccc gaaagagcac ctctcaccgg tgatcgagag
     2821 gaccacggac gaccactaca caacggtcat cttcgtggac gagggcatca gcccgcccgg
     2881 agaactgagg gtcaccctca ggttgacagc ggggtgcgac ctcttccacg ggatggcgga
     2941 ggacacctac cggacagagg tgaaggaaac tattcccgcc gggacagcgg ggcggatcgt
     3001 cgtcgatgcg atggtcggga agtattgatc acccggaccc tggaggggga gatttctgga
     3061 tcgtggaggt ggcgttgttg agcctctggc gggcacgggc cacatagata taccaggtga
     3121 catccggtca gaacccccgg tacaccctgc ccacggggcc tcttcgggtc gccttcaccg
     3181 gatcagggag taatctctca acctcccggg acatgggccg agttcactct gccgccgctc
     3241 tcgaaaagtt aatccgatat tacaagtatt ataggtatta taggtattac aagtaataca
     3301 gatacggata ctacagggaa gggatcaaca tggtgtatgc gccagataac cggcaccaga
     3361 aggagaggtc cagggaatcc atccgcatgg gactgactcc gtttgagatg cgtgcactcg
     3421 aggcttctat caagaagaac cggaaagcgc tggaactctt aagccagcac tgaaaagaga
     3481 tctatccttt ttgtatttgc gcggatccat tcttcgacct cgtccagggt atacctgctg
     3541 ttgccgtcgt cgtcgaggtc ggccagcgtg accatgaacc ggtccacctc ttctgcgtct
     3601 gcatcaaaaa tgtacccgtt tgcgcgcagt ataagtacgt cagtggccgt atacgcagtt
     3661 cgcttattcc cctgccagaa cccatgtccg tttgtgacga cctcaaggaa ccaggcggcc
     3721 ttcctgtaga tgttctgctg acatacccag taattattcc agagatagac ccaggtctcg
     3781 acacaaccgg gatccttcag cccctcccat ccccggtctt caggccggtc gtgtaacgca
     3841 ataagggtgt cgtggattgc gataatcttc tctccggtga tgtcattcac gagccagccg
     3901 caccgcggac accaccaacc gatcgggatc tcggccctgt ggccgtcttc atctgtatcg
     3961 cggatgaagg cccggatcat cctgacatca tcacaataag ggcttggaca gtcgggggcg
     4021 agacgcagca tgctcatcag atcctattga aaatggcaac gcccgggtag atgaatttaa
     4081 tccctggtgg gcttcgtgga caagagtatc ggctcgccag gagaaccgat ggtcgcccgg
     4141 accctgaagg ggggagacgg tgtcccaggc gatgtcagaa cccccgaggg gccgaccgcg
     4201 acgacatgga tcaccaggag gtcgtcgatg accgagagga ttgtccggta atccccgacc
     4261 ctgagcgagt agaacgcggg gttgtctgac cctttcagtt ttttgagatg cctcttcggg
     4321 tcagcctcac cggcgagtgc tgcgagttcc tcaccaatcc gcactgctat gggtcgtgga
     4381 atgctcctcg aggttgtgac gggcctcgcg gtgatgacca cgcggtaggt cattcctcct
     4441 tcagatccgc catggcctca tccagagtgt agacccttcc ggccttaaga tcctcgagcg
     4501 accgttcgag gtccctcccg tattgtctct tcactgagcg gctccttgtt gactgccata
     4561 accaccagac gctcgattac ctcgctgtac gactcccggg gatgaatctt cagggcgttg
     4621 agtcgtccct tgagttcggt gtcgatcttg atggtggtgc tagtcatact gaggtacact
     4681 tcggtttacc caatggaggt ttgcattggc ggatgccgtg cggtatcctg cggtggtccg
     4741 ggtcggtcat ggcgacagtg ccctgccccc accacgtccg catctacccc ggatgcgata
     4801 agcgaccggt caaatgatgc tgtttgaaaa tctccaggat cgctctgggt agttgtattc
     4861 aggaaaacat gcccatgtct aaccccgaca ggacatcgtg gccccgggcg gggaggtccc
     4921 ctccggcgcc agggtgcccg gtgacgggga ggtctcccat ataccggttg aggaactcca
     4981 gggcaccagt ggagagcctg tagcagaagg ttctctcctc cctatcgacg gtgacgattc
     5041 cttcgtcctc gaactgctgc atctgccagg agaccgtcgg gccggagagc ccgagtcgtt
     5101 ctgcgatctc gtttcgggtt gctcccggca acctgagaag gtgtgccagt atcgcccccg
     5161 gggtctcgtc ccggaggctg gcgatgatgc gcttctcgag ggcggtatgc cgctggttcc
     5221 gcgcaaagtg ccggaagcgg ttccggtagt ccacggcagc caccttgcct tccctgatga
     5281 gcatatcgag atggtagcgg agccgccctc gcgatgcgcc cgtcaacttc aggagttcac
     5341 aggtgctggc accggagtgg atagtgatct gccgctagag gtcgcgcctg aagtcgtcgt
     5401 tgaggatgtt atggcgcgtc acccaccgga acccgagcgg caggaggagg ctgagcgcga
     5461 cggcggcgac gccgatgaac gcaaaaaccc cacgaaaatc aggcgtttgt tcccagactc
     5521 catacgatca ccacaccgga gtattctgcg ggctcctaat ctatcggaac gaggggcccc
     5581 gcgggcacat cctttgaact gtatatgacg tgcaacgggg acgccgactg ctcggcacgc
     5641 gcacggggct cgaccgggat aatccagaac gtctcgttgc cgaagactat gctcccgacg
     5701 aggacgttgt tcccgatcgt caggaggata tcgctttcgt caacgccgac gatcacccct
     5761 tcgtaggtgt tgataccgtc atcgatctgt tcgaagttca tcccgtgctg ttcggcccgg
     5821 tacgccatac catggataga aagcgggaat ccatcccgga tctctcgcga gagggcagcg
     5881 tggtcgaacg ccacaacgtc gtaccgcttg atgcccttcg ggatctgcag ttcttccggt
     5941 gccgggatcc cggcggccgc gagatcggag agcgacgcgg cgcccgtgac gagcacggtc
     6001 atggtgtcgc cgatgaccag aggcggggtg ttgtcaccca cggctggaga tcccgtccca
     6061 tcctgagcgc atgaaaccag cgcgatcgcc actacaacga tcaggcatac caggagggcg
     6121 atgcctccca cgtgagattg cttcattctt tttcctctgg tgcatctccc gggacaggtg
     6181 cacccgggtt ttccacgtgg gagaggtcga cctcctccta taagcgatct ctgtgacacg
     6241 cgtctacatg tcgggcccct ccgatcactc ccagaccaaa aacctcctgt acaaaccccg
     6301 gaacgggagg cgcgccgaac ggcgggagta actcgtgcag ggaaccgcca gaaaaatcag
     6361 gagaacgtga ggagaggggc gttagaggtc gtgcacgggc acgcatctcc tctccgcata
     6421 cggcatgacc ggcccggcga ggtcgagccc gagcgtctca cgcaggacat cctcgatggt
     6481 atccgcggtc acgaacgcaa gttcgcgccg cacctcctcg ggcacgtcct caagatcacg
     6541 ctcgttctcc cgcggcagga tgaccgtctt gatcccggcc cggtgtgccg cgaggacctt
     6601 ctccttgatc ccgcccaccg gcagcacggc gccagagagg gtcacctccc ctgtcatcgc
     6661 gatcgtagga tcgaccgctc tgccggtgac gagggaggca agcgccgtaa agatggtcac
     6721 accggccgat ggcccgtcct tcggggtcgc ccccgagggc acgtggatgt ggatgtcgct
     6781 tgagaagaaa tcaaaccggc tcggcatgcc tgccagtcta gagcggatca ggctgagcga
     6841 gatctgggca gactccttca tcacgtcccc gagttgaccc gtaagtgtga gtttcccctt
     6901 accgggcatg aacgtcccct caataaagag gatgtccccg cctaccggtg tccaggcgag
     6961 ccccgtcacg acgccgggcg ggttttcctt ccttgctaca tcctgcctga cggtctctct
     7021 accgagtatc tccgggagca tatccgccgt tacgacgatc ggaaggtcgg ccgtccccga
     7081 gacgatcttc gtggagacga accgcgcgat cctggccagt tgtttcttga gaccacgcac
     7141 acctgcttcg cgggtgtagc ggtcgatgat cgcctcgatg gcgccgtcat cgatctggag
     7201 ttgctcctca tccaggccat gctccgccag cgtctccggc aggaggtgat cttttgcaat
     7261 cgcgaacttc tcgttcttcg tgtagcccga gatctcgatc acctccatcc ggtcaaggag
     7321 cggcgccggt atggtggaga gcgtgtttgc cgtggtgatg aagaagacat ccgagagatc
     7381 gtaggggacc tccaggtagt ggtccgagaa cgtgctgttc tgctccgggt cgaggacctc
     7441 caggagggcg cttgccgggt cccccaggta cgaagaaccg accttgtcga tctcgtcaag
     7501 gacaaagaca gggttcttcg ttccggccct ccggatcccc tggatgatcc ggccgggaag
     7561 cgccccgaca tacgtccgcc ggtgacccct gatctctgcc tcgtccctca caccgccaag
     7621 actgatcctg acatacttcc ggccgagagc ctccgcgatg ctccgtccga ggcttgtctt
     7681 gcccgtgccg ggcggcccca ccaggaggag gatcgagccc tgcttctcct gtttgagctt
     7741 catgaccgcc aggtgctgga ctatccgctc tttgaccttc tcgaggccgt tgtggttgct
     7801 gtccaggacg cggcgagcct ccacgatatc gatctccttc gtctccccaa ctgtccaggg
     7861 caggtcgagc aggaggtcga ggtagttcct tatcccctgg ctctcgtggt gctggctccc
     7921 gcccatctca agttttttga gttcggaaag tgccttcttc cgcacctcat ccggcatcgc
     7981 cgagcgctcg atccgctccc ggtagtcatc atcgccggtg gaaccctcgc cttcgcttag
     8041 ttcttcctgg atcacgcgaa gctgctccct gagcagcgcc tcgcgatttg ccttccccac
     8101 cttctcggtg gccttccttg caacctcgat ccggaggttt agacgctcgt taaagtccac
     8161 cagtaaactg aggaacgcgt tgtagcgctc ttttacagat acggtctcaa gcagcgactg
     8221 tttcgttgca acgtccaccg gcatgaaagg catgacgaaa gccatgatct ggtcgacgga
     8281 ctccataccg tcaacggggc gggtgaactg ctcggagccc tggaagttgc tgctgatctc
     8341 gtggatggcc gccctgacgt cgcgaagcat ctcctcccgc ccctcctcat cgaggtcgtc
     8401 tatgtccggg agaggttcgc aggaagcgtg atacacccct tcgatctccg agagtgccac
     8461 cgcccgcaca cgcgaggttg catgcgcaaa gactatgtaa ccctcgtctg caggctgcat
     8521 gcgcatgatg gataggagtg tcccgaccgt gtagaacgcg tcaaccggat tctcaacggc
     8581 ctccgcatcg ctctttgcgg taagtccaac cgcggatgcc gagcttccgc tcttcgttgc
     8641 ggcgatcaga gcttccccga cggttttccc gaccaggaac tttgtccggg tctccgggta
     8701 gaccacaacc tcggagagcg gtatgacaat gtgttcgttg ctggtgtctt ttgaatacat
     8761 gatgattttt tcctgattat ctggttcgga attactttgc acgaaggtcg actcgagatc
     8821 agccgacctt gcggaggacc tccaccagga gatccacctc cttctcatcg agcgcctgca
     8881 ccatcctctc gaccatccgc tgcagcgctt ttcggtcagc gtttgccagc atccgcccct
     8941 tctcggtcaa ccggatgtag gttactctcc cgtcatcggg ggatttctga cggtaggcgc
     9001 actccatccg gacgaaccga ttaaccatct ccgtcacggt aggctttgag tttctggtga
     9061 tctcggcaag cctgctgaac gtcacatcgc cgtgcctgtc gatgaccttc aagtaggaga
     9121 tctgtttcgc cgtcacatcg ccaagcccgc actcggagag gatctcacag gagcactcgt
     9181 ccctgatggc aaagaggcgt tcaaacacct cgaacagacg ctcacctctt gccgacatat
     9241 cagatgctta gttagtttgg atagacctaa ctataaaggt tgtgat
//
LOCUS       DAOSZX010000054         9254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6363_length_9254_cov_20.719426, whole genome shotgun sequence.
ACCESSION   DAOSZX010000054 DAOSZX010000000
VERSION     DAOSZX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6363_length_9254_cov_20.719426"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PK196_05570"
     CDS             complement(<1..332)
                     /locus_tag="PK196_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40920.1"
                     /translation="MRPDEQNDIIDAAIRILLEEDRCINVGFSPDGVSIRFPTTRKLA
                     EFLGIPHYYVLPRFGEMERDGLIRREERVGISTTPAGTQRLLSIMAERYSERAEEVLG
                     RDIFAVLR"
     gene            complement(386..622)
                     /locus_tag="PK196_05575"
     CDS             complement(386..622)
                     /locus_tag="PK196_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40921.1"
                     /translation="MKIYVRERQKVGTGVKQPRFRIVAILEEQGEQKHLKVEGTHFRK
                     VEIEQIAEDVGAEVVYLETMPEEERGEMKKAEAA"
     gene            711..1514
                     /locus_tag="PK196_05580"
     CDS             711..1514
                     /locus_tag="PK196_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HON40922.1"
                     /translation="MAERFEIESRLRGESLVRRGMLRRTGGSTIRIMPDLNVIKIGGH
                     GVIDYGREIVYPLIEEIGELSRRHKILVATGGGARVRHILDLGIDLGMPTGVLAELAG
                     KISEQNAIMISILFSKYNGVRIHTEDLLSLPSLISLGMLPVVQGTPPYGLYEHPPKLG
                     TIPPHRTDTGAFLMAEVVGAKNCILGKNVDGLYTENPFENPDAELIPEITAEELLEME
                     MEDMVLEPMAVELLRDAVYVREIKVVNAHTPGNITKAVNGERVGTLIRA"
     gene            1558..2259
                     /locus_tag="PK196_05585"
     CDS             1558..2259
                     /locus_tag="PK196_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON40923.1"
                     /translation="MTIYIGIDDTDTRESRGTGRLARAIAAELSRSYGIFGVTRHQLF
                     LHPSIPYTSHNSAAVIHILERENGVTADDLFETAKELLLADFIDGSDPGICIAADRQM
                     GQEVTRFGFAAKREVVTQEEARTFARQAGILLEGLGGTEDGVIGALAGVCLAASGNDG
                     RFVQKGTTRSLHGTSTVAAILASGVDRVMTRDGAAVSEGLVCLKKFPKPAFVGGEAIL
                     YVEGENGVYRDIVVG"
     gene            2400..3329
                     /locus_tag="PK196_05590"
     CDS             2400..3329
                     /locus_tag="PK196_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HON40924.1"
                     /translation="MMKKRIIFAVAAVMVAILLICGCTGTETEQPPAEKQQLRIATTT
                     SLYDTGLLDHLKPMFEDEYNAEVLIISAGTGKAIEYGQRGDVDVLMVHDRAREDAFIA
                     DGYGINRRVFAYNYFIIVGPESDPAGIKGMEPEEAFTTIREKGMAGEPGVIFVSRGDA
                     SGTHSKEQAIWKAAGFNYSTEIQGSGDWYREAGTGMGATLEMANELQGYTLSDIGTFL
                     AYQRDLDLVTLVDEGDILLNVYSVMQINPEKYPEINSTLAKAWINFLISDEVQEEIAS
                     FGVAEYGQPLFYAAKDAWEKIGVTEAEVKDPVL"
     gene            3452..4162
                     /locus_tag="PK196_05595"
     CDS             3452..4162
                     /locus_tag="PK196_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON40925.1"
                     /translation="MYEIIEGFIEAIRLIVTLNPNVMAIAARSIIISLTATIIASLIS
                     LPLGAAINFGNFRGRKGLINLIQTLYSLPTVIVGLLIFLLISRAGPLGFLRLLFTPTA
                     MIIAQTVLIIPIMTGLTISALSGVDPVIRDTLTSLGATRFQFLINIMKEARFAILAAV
                     AVGFGRAISEVGAAILVGGNIAASSFMSSTRVLTTAISLETSMGNIPQSIALGIVLLI
                     IALGVNLVISTLQQGRNP"
     gene            4159..5247
                     /locus_tag="PK196_05600"
     CDS             4159..5247
                     /locus_tag="PK196_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON40926.1"
                     /translation="MIELDGVSKEFDGKRVLDGVTGRVDDGEIFAVIGPSGAGKSTLL
                     RLIGLLDTPTAGTIRINGIDIHASKEQSLRIRRTIGMVFQKPAVFNTTVAENIAIGLR
                     FRGVERETIMKKVRGALDMVGLAGYEERRARTLSGGEMQRVALARAMVIEPEILLMDE
                     PTANLDPVSVEKIEELVIRINRELGTTILFSTHDMYQGQRLAHRIAVLMNGRFAQVGT
                     PREVFTLPATRDLARFVGIENILEGVVMGREDGGIASIDVGSTRVRARSSFGPGERVA
                     LCIRPEDMQIAPAERGVGNPGRNTLQGTIISIVPRGPFSRVTVDCGFMLSSILSWKKV
                     DDLELQEGSLVVVSFAPESVHVVSAGES"
     gene            complement(5285..6679)
                     /locus_tag="PK196_05605"
     CDS             complement(5285..6679)
                     /locus_tag="PK196_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HON40927.1"
                     /translation="MSGTEGSWQAEAGRIAAALDGGRARVDLERLRPPVNTYLVRSLD
                     KTALRFSLPVLLTPPPGTASHPGADGAVWAVIEAVKAAVPVEPALGPIAGLGTEHPVH
                     CQQNVEPVTLIASPGAIGTDLWRPGDDNRIDSRGLHLVVRGALPYPGPPGRGTEREAA
                     DRLGELLEAVDRVARRVPAAEIAAACALSLDQKALRRALPGVGLVAFIADGTRPARRF
                     TRLRGHHRIAGPKEGVHVPFRCPRELDPIEVELEGSGRVVTGLGLRRREVFAVAGSNA
                     EGKSTLLQAIVAGQDDHAAGDGRELLVSVNGVAGAEANEQELVGADVSLFFQSLPPGL
                     SGDPRAAYGRGSGSLVMAEKIQAALRAAAPILIIDEDRAATNLLVPGCLQSGEVTPLS
                     TLLATSRQAIGDTTILFAASSLDILIAQADRILLFSGHEAQALDPREFRRRLDRHLAG
                     VRELLAAQERMDDC"
     gene            complement(7053..7967)
                     /locus_tag="PK196_05610"
     CDS             complement(7053..7967)
                     /locus_tag="PK196_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HON40928.1"
                     /translation="MGEERWCLVVSGQEAEERRRRLLEAGLLDRRYRPRQEGGTVLLP
                     LTGEFPGAVRREFEAVPERPNLPRHEIVGGIAIMQENDPAGAGRVLASRPSLHTVLFP
                     ETPVEGEYRTRRFAVLAGVPATRTRVTEYGLRFDVDLSLAYFSARLSTERQRILAAME
                     KGERVLDMFAGVGPFAITLARKADIVVAADINPAAVHLLAGNISLNRMSNVVPVLADA
                     GHLPRYGFRPFDRVVMNLPMAAPRYLPEAAALCRDGGTIHLYALQEREGEHLPLIRGV
                     TRGEVLERRVRTYSPGKWHAVYDITLER"
     gene            8015..9223
                     /gene="hmgA"
                     /locus_tag="PK196_05615"
     CDS             8015..9223
                     /gene="hmgA"
                     /locus_tag="PK196_05615"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HON40929.1"
                     /translation="MDEYIERLKDGSLKLYALEKELPPEEAVRVRRAFIEGETGTPLP
                     AIGSFTIGIDRVVKRNIENMIGTVQVPVGVAGPLMLHGEYASGPYYIPLATTEGALVA
                     SVNRGCSLITRAGGADVRIMRDGMTRAPVFATRDIIHAKETADWVETHTAEIREAAES
                     TTKHGEFLKARTYIAGTSVFVRLEFDTKDAMGMNMVTIASQKVGELIERETGARLVSL
                     SGNMCTDKKPAAINLVQGRGKTVVAGVRLSEALIADLLKTDAETLLEVNYRKNLVGSA
                     RAASFGFNAQAANVVAGIFIACGQDAAHVVEGSTAITTVDRVEGGVYVSVSIPSLPVG
                     TVGGGTGVETQHECLAMLGVAGGGDPPGANAKAFGEIVAAAVLAGELSLLGALAAQHL
                     ARAHQNLGRG"
BASE COUNT         1925 a         2881 c         2785 g         1663 t
ORIGIN      
        1 tgccgcagca ccgcgaagat atcccggcca agtacctctt ctgcacgctc gctgtatcgc
       61 tccgccatga tagagaggag acgctgcgtc cccgctggtg tggtggagat cccgacccgc
      121 tcctcccggc gtataaggcc gtcccgctcc atctccccga accttggaag gacgtagtaa
      181 tgtgggatcc ccaggaactc tgcaagttta cgggtcgtgg gaaaacgtat ggagacgccg
      241 tccggggaaa acccgacgtt tatgcagcga tcctcctcaa gaaggatcct tatggccgca
      301 tcgatgatat cgttctgttc gtctggtctc atccgatcgc accattgtct atcacgaccc
      361 tgcaaaaaag atgacggccg gagagttacg ccgcctctgc tttcttcatc tccccgcgtt
      421 cttcttcggg catcgtctca aggtagacaa cctccgcccc cacatcctct gcaatctgct
      481 ctatctcaac ctttcggaag tgcgtcccct caaccttcag gtgcttctgc tcgccctgct
      541 cttcaaggat agcaacgatg cggaaccttg gctgtttcac accggtgcca accttctggc
      601 gttcacgtac atagatcttc atctctctca ccccatacca actggtatag cagtagatat
      661 acgccaaaga tacttaacct tttcccatca tacagatagg aggaaagatc atggcggaac
      721 ggtttgagat tgaatcaagg ctgcgcggcg aaagtttggt caggcgagga atgctccgca
      781 ggacaggggg ttccacgata cggatcatgc ccgacctgaa cgtgatcaag atcggcggcc
      841 acggagtcat cgattacggc cgggagattg tatacccgct gatcgaggag atcggagaac
      901 tttccaggcg ccataagatc cttgtagcca ccggcggcgg cgcccgcgtc cgccacatcc
      961 tcgacctcgg catcgacctc ggtatgccca cgggggtgct tgcggaactt gcgggcaaga
     1021 tcagcgagca gaatgcgatc atgatctcca tcctcttctc gaaatacaac ggcgttcgga
     1081 tccataccga agacctcctg agtctgccct cactgatctc gctcgggatg ctcccggtcg
     1141 ttcagggcac accgccatac gggctctacg aacacccccc aaaacttgga accatcccgc
     1201 cgcaccggac ggataccggc gccttcctta tggcggaggt ggtcggcgcg aagaactgca
     1261 tccttgggaa gaacgtggac ggcctctaca ccgagaaccc ctttgagaat cctgatgccg
     1321 aactcatccc ggagatcacg gccgaagaac tcctggagat ggagatggag gatatggtgc
     1381 tcgaaccgat ggcggtcgaa cttctccggg acgccgttta cgtcagagag atcaaagtgg
     1441 tgaacgccca tacccccggg aacatcacaa aggccgtgaa cggggagcgg gtcggcacgc
     1501 tgatccgggc gtagcctccc gggttaaacc gaccggcagg gcagacgagc gataacaatg
     1561 accatctata tagggattga cgacaccgac acccgcgagt cccggggaac cgggcgactc
     1621 gcacgcgcga ttgcagctga actctcccgc tcctatggga tcttcggggt gacacggcac
     1681 cagctctttc tccatccttc catcccctac acctcacaca acagcgccgc ggtcatacac
     1741 atactggaga gagagaatgg agttacggcc gatgatctgt tcgagacagc aaaggaactc
     1801 ctgctcgccg atttcatcga tgggagcgac cccggcatct gcatcgccgc cgaccggcag
     1861 atgggacagg aggtcacccg ctttggtttc gcagcaaaga gagaagtcgt gacgcaggaa
     1921 gaggcgcgca cgttcgcccg tcaggcaggg atactcctcg aaggacttgg cggcaccgaa
     1981 gacggggtta tcggcgcgct ggcaggtgtc tgcctcgcgg catcggggaa cgacggtcgg
     2041 tttgtccaga aggggactac acgaagcctg cacggaacct caaccgtggc cgcaatcctc
     2101 gcctccggcg tcgaccgggt aatgacccgg gatggtgctg ccgtcagcga aggcctcgtc
     2161 tgcctgaaaa agttcccaaa acccgccttt gtcggaggtg aagcgatcct ctatgtggaa
     2221 ggggagaatg gggtctaccg cgatatcgtg gtgggatgag atcaggcggc ctgccgacga
     2281 gggtaacagg atcacgttta ctcagcctga gataaaacaa agtcaactaa attgaacaca
     2341 cattaacaca aaacacggct atttaaatgc aggcagcaca ataccagttc ttgatagaca
     2401 tgatgaaaaa gagaatcatc ttcgccgttg cggctgtcat ggtcgccatc ctgctgatct
     2461 gcggctgtac cgggacagaa accgagcagc cccccgcaga gaagcagcaa ctccgtatcg
     2521 ccactacgac aagtctctac gacaccgggc tcctcgatca cctcaaaccc atgtttgaag
     2581 atgagtacaa cgccgaggtc ctgatcatct ccgccggtac cggcaaggcg atcgagtacg
     2641 ggcagcgggg cgacgtggac gtcctgatgg tgcacgaccg tgcacgtgag gatgccttca
     2701 ttgccgatgg ctacggcatc aaccggcgcg tctttgccta caactacttc atcatcgtag
     2761 gcccggagtc cgacccggca ggcataaagg gcatggaacc cgaagaagcg tttacaacca
     2821 tacgcgagaa gggtatggcc ggtgaaccgg gtgtgatctt tgtctcgcgc ggcgatgcgt
     2881 caggcacaca ctcaaaggag caggccatct ggaaggccgc cgggttcaac tactcgacag
     2941 agatccaggg ctccggcgac tggtaccgcg aggccgggac cggcatgggt gcaacccttg
     3001 agatggcaaa cgaactgcag ggctacaccc tctcagacat aggcaccttc cttgcctacc
     3061 agcgtgacct cgacctcgtg acactcgtgg acgagggcga tatactgctc aacgtctact
     3121 ccgtcatgca gatcaacccc gagaaatacc ctgagatcaa cagcacgctg gcaaaagcct
     3181 ggatcaactt cctgatctcc gatgaggtgc aggaagagat cgcctccttc ggcgtcgcgg
     3241 agtacggcca gccgctcttc tacgctgcaa aggacgcctg ggagaagatc ggtgtgaccg
     3301 aggccgaagt gaaagacccg gttctctgat ccggtcaacc catccccttt ttgcatgaga
     3361 ttgaacccct atcttcacca agatctctct gacccgaccg gggcatcatc tggttttttt
     3421 gccgcttagg tagcacgatc aggtgcaggg catgtacgag atcatcgagg ggttcatcga
     3481 ggcgatcagg ctcatcgtca cactcaatcc caacgttatg gccatcgccg cacgaagcat
     3541 catcatatca ctcaccgcaa ccatcatcgc ctcactgata tctctcccgc tcggggcagc
     3601 gatcaacttc gggaatttca ggggcagaaa gggtctcatc aacctgatcc agacgctata
     3661 ctccctcccg acggtcatcg tagggctcct gatcttcctg ctcatctccc gcgccgggcc
     3721 gctcgggttt ttaagactgc tcttcacccc gaccgccatg atcatcgcac agacagtcct
     3781 catcataccg ataatgaccg gtcttacaat atccgcgctc tctggggtcg atccggtcat
     3841 cagggacacc ctgacctcgc taggggcgac acggttccag ttccttataa acatcatgaa
     3901 agaagcccgt ttcgcgatcc tggcggccgt cgcggtcggg ttcggccggg cgatatccga
     3961 ggtaggggca gccatcctcg tcggcggcaa catagcggcg tcatctttta tgagttcgac
     4021 ccgcgtcctg accaccgcga tatcgcttga gacgtcgatg ggcaacatcc cgcagtccat
     4081 cgctctcggg atcgtcctgc ttatcatcgc tctcggggtc aatctcgtca tatcaacgct
     4141 gcaacagggc aggaatccat gattgaactt gacggagtct caaaagagtt cgacggcaag
     4201 agggtgctcg acggagtcac cggcagggtt gatgacgggg agatctttgc cgtcatcggg
     4261 ccgagcgggg ccgggaaatc aaccctgctg cgcctgatcg gcctcctgga cactccgaca
     4321 gcagggacca tccggatcaa cgggatcgat atccacgcca gcaaggagca gagcctcagg
     4381 atccgccgga cgatcgggat ggtctttcag aaacctgcag tctttaacac gacggtcgcc
     4441 gagaacatcg ccatcggact caggtttcgg ggggtggaga gggaaacgat catgaagaag
     4501 gtcagggggg ctctcgatat ggtcgggctt gctggatacg aggagcggcg ggcacggaca
     4561 ctctccggcg gcgagatgca gcgggtggca ctcgccaggg cgatggtgat cgaaccggag
     4621 attctcctca tggatgaacc gaccgcaaac ctggatccgg tctcggtgga gaagatagag
     4681 gaactggtca tccggatcaa ccgcgaactc ggcacaacga tactcttctc gacccacgac
     4741 atgtaccagg ggcagcggct cgctcaccgg atagcggtgc tgatgaacgg caggttcgcg
     4801 caggtcggga cgccaagaga ggtcttcacc ctcccggcaa ccagggatct cgccagattc
     4861 gttggcatag agaacatact ggaaggtgtg gtcatgggca gggaagacgg agggatagcc
     4921 tccatcgatg tcggcagcac tcgggtgaga gcgcggtcgt cgtttggccc gggggagagg
     4981 gtcgccctct gcatcaggcc ggaggatatg cagatcgcac cggcggagag gggggtgggc
     5041 aaccccggca ggaacacgct ccagggcacc atcatatcca tcgtgccgcg cggtccgttc
     5101 agcagggtta cggtcgactg cgggtttatg ctctcatcta tcctctcctg gaagaaggtg
     5161 gatgacctgg aacttcagga aggcagcctt gtggtggtct cgtttgcgcc ggaatcggtt
     5221 catgtcgtca gcgctggcga gtcctgaata cccgaatacg gcctttccgc cggcaaaccg
     5281 ggtatcagca gtcgtccatt ctctcctgcg ccgccagaag ttctcgcacc ccggcaaggt
     5341 gcctgtccag tcgccgccgg aactcccggg gatcgagcgc ctgcgcttca tgaccggaga
     5401 agagcaggat ccgatcagcc tgcgctatca gtatatcgag cgaggaggcg gcaaacaaga
     5461 tcgtcgtatc cccgatcgcc tggcggctgg tggcaagcag cgtggagagc ggtgtcacct
     5521 caccgctctg gaggcacccg gggaccagca ggttggtcgc ggcgcggtcc tcatcgatga
     5581 tgaggatggg cgccgcagca cggagcgctg cctggatctt ctctgccatc acgagggatc
     5641 cgctcccgcg gccgtaggcc gcccggggat cgcccgatag gccgggcggg aggctctgga
     5701 agaagaggct gacatccgca cccaccagtt cctgctcgtt tgcctccgcc cccgcaaccc
     5761 cgttgacact gaccaggagt tcacgcccgt cgccggcggc gtggtcatcc tggccggcaa
     5821 cgatcgcctg gaggagggtg ctcttcccct cagcgtttga accggcaacc gcgaagacct
     5881 ccctgcgccg gagtccaagc cccgtcacca ccctgccgct cccctcaagt tcgacctcga
     5941 tcgggtcgag ttcccgcgga cagcggaaag gaacgtgaac accctccttt gggcctgcaa
     6001 tccggtggtg accccggagc ctggtgaacc ggcgggccgg gcgcgtgcca tcggcaatga
     6061 acgcgacaag ccccaccccc ggcagggcgc ggcggagggc tttctggtca agggaaagag
     6121 cacaggccgc ggcaatctcc gccgcaggca cccggcgcgc tacgcggtcg acggcctcaa
     6181 ggagctcacc caggcgatca gcggcctccc gttccgtgcc ccgcccgggc ggccccgggt
     6241 aagggagtgc acccctgacg accaggtgaa gaccccgcga gtcgatccgg ttatcatcgc
     6301 ccggtcgcca gagatccgtt ccgatggcgc ccggtgaggc gatcagcgtg acaggttcca
     6361 cattctgctg gcaatggacg gggtgctccg tcccgagacc tgcgatgggg ccgagtgcgg
     6421 gttcgaccgg gaccgccgcc ttgacggcct cgatcaccgc ccagaccgcc ccgtcagcac
     6481 cggggtgact ggccgtgccg ggcggcggtg tgagcaggac gggaagactg aaccggagcg
     6541 ccgttttatc gagcgagcgg acgaggtagg tgttgacagg aggcctcagc ctctccaggt
     6601 cgacacgcgc ccggcccccg tcaagagccg cggcaatccg gccagcctca gcctgccagg
     6661 agccctccgt tccgctcata cacactcatc tgatagttct gccccggagc ccatctctct
     6721 tttcacgcga ctgcactccc tggtgaacgc cttacgctga acatgaacac ccccatctcc
     6781 tcaggcttcc gggacgccat gccctttcag tatccttgaa cgccacgatc gcacggccgc
     6841 tccccctccg ggggaagtgt gtggcgattg gccctggaaa gccgtgcccg gcgcgcagat
     6901 ggatctcctg cggttctaac cgggaaccga ctaaaaaccc atttccggac gctgcagcaa
     6961 gggagaatca caccaccacc ccggaatccg tccgaagaga ggcttatcaa acccggcaga
     7021 tcagcctgaa ggtactggtt tgatagcctc ccttacctct ccagggttat atcgtaaacc
     7081 gcatgccact ttcccggaga gtatgtcctg acccgccgct cgaggacctc accccgggta
     7141 accccgcgga tcagagggag gtgctcccct tcccgctcct gcagggcata gagatggatc
     7201 gttcctccgt cccgacagag cgctgcggcc tcagggaggt accgcggcgc ggccatgggg
     7261 aggttcatga caacccggtc aaacgggcga aacccatacc ggggaaggtg accggcatcc
     7321 gcaagcaccg gaacgacatt gctcatccgg ttgaggctga tgttgccggc caggagatgg
     7381 acggcagcag ggttaatgtc cgccgcaacc acaatatccg ctttacgggc cagggtgatc
     7441 gcaaaaggcc ccacaccggc gaacatatca agaacccgct cacccttctc catcgccgca
     7501 agaatccgtt gccgctcggt ggagagacgg gcagagaagt aggcgaggga taggtctaca
     7561 tcaaaacgga gtccgtactc cgtcaccctg gtgcgggtgg cggggacacc ggcaagaacg
     7621 gcaaacctgc gggtccggta ctcaccctcg acgggggtct cggggaagag cacggtgtgc
     7681 agcgacggtc ttgaggcgag cacccgcccg gcgccagcgg gatcgttctc ctgcatgatg
     7741 gcgatcccgc cgacgatctc gtggcgtgga aggttggggc gttccggcac cgcctcaaac
     7801 tcccggcgca ccgcaccagg aaactcaccg gtaaggggga gaaggacggt accgccctcc
     7861 tggcgcggcc tgtaccgccg atcaaggagc cctgcctcaa gcagtcggcg ccttctctct
     7921 tcggcctcct gccctgacac caccaggcac caccgttctt cacccacgga ctccaccacc
     7981 cgccatcttt acgtataaga tctcatactt attcatggat gagtatatcg agaggctgaa
     8041 agacgggtct ctcaagctct atgcgctcga gaaggaactc cccccggaag aggccgtaag
     8101 ggtgcggcgg gcgttcatcg agggcgagac cgggacacct cttcccgcga ttggatcgtt
     8161 cacgatcggg atcgatcggg tcgtgaaacg caacatcgag aacatgatag ggactgtcca
     8221 ggttccggtc ggtgttgcag gaccgctcat gttgcacggc gagtacgcca gcgggccata
     8281 ctacatcccc ctcgcgacga ccgaaggagc acttgtcgcc tcggtgaacc gcggttgttc
     8341 gctgataacc cgggcgggtg gtgcggatgt ccggatcatg cgcgacggga tgacccgggc
     8401 accggtcttt gccacccgtg atatcattca cgcgaaagag acggcagact gggtggagac
     8461 gcacaccgcc gagatccggg aggccgctga gagcaccaca aagcacgggg agttcctcaa
     8521 agccaggacc tacatcgccg gcacgagcgt cttcgtcagg cttgagttcg acacgaagga
     8581 cgcgatgggc atgaacatgg tgacgatcgc cagccagaag gtgggcgaac tcatcgagcg
     8641 cgagaccggt gcaaggctcg tctccctctc gggaaacatg tgcaccgaca agaaacccgc
     8701 ggccatcaac ctcgtccagg gcaggggtaa gacggtggtt gctggggtgc ggctcagcga
     8761 ggcgctgatc gcggatctcc tgaagaccga tgcggagaca ctgctcgagg tcaactaccg
     8821 caagaacctg gtgggctccg cgcgggcggc ctccttcggg ttcaacgccc aggcggcaaa
     8881 cgtggttgcc gggatattca tcgcctgcgg tcaggacgcg gcccacgtcg tcgagggcag
     8941 caccgcaatc acaaccgtcg accgggttga gggtggtgtt tacgtctcgg tcagcatacc
     9001 ctcgctcccc gtcgggactg tcggtggagg aaccggtgtt gagacccagc acgagtgtct
     9061 ggcgatgctt ggtgtcgcgg gcggcgggga cccgccggga gccaatgcaa aggcttttgg
     9121 agagatcgtt gccgctgcgg tccttgccgg cgaactctca ctcctcggcg ccctggcagc
     9181 ccagcacctg gcccgggcgc accagaacct ggggcggggg tagtagagag aacgtccagg
     9241 caccctggcg ggag
//
LOCUS       DAOSZX010000055         9185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6418_length_9185_cov_19.569003, whole genome shotgun sequence.
ACCESSION   DAOSZX010000055 DAOSZX010000000
VERSION     DAOSZX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6418_length_9185_cov_19.569003"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1254)
                     /locus_tag="PK196_05620"
     CDS             complement(58..1254)
                     /locus_tag="PK196_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HON40930.1"
                     /translation="MTKWQGLVLSSVLTLVFIGLAPLLQPPWLLTLFLVLFSLLLSLI
                     RDTRYVSLSIIALAVLYGLGWLSTFVFTCTLGIVVFGEVAFRLTGGEHASYLYHMLAA
                     IVVSIAVMFYLHYTVALVALMGVVAAVLLRAVLGGRDDALMVEALGVAMTMFLFEELK
                     YEVDLSILIAAAVIAFGFGYTSYRLRVADISGLFSGAMIGIILIVFADVRWFLIMLTF
                     FIIGAAATRYRYADKERFGVAQEHGGVRGYFNVFANGLVATGGAILYGITGNPAFAAL
                     FMSSVASAAADTAASEIGVTGKTPYLITTFKPVPRGTNGGVTLRGEVAAILASIIVAA
                     VALVMGVADPKLAIVTVIAGFIGTNVDSVVGATLENSGRIGNSGTNLTATFFGGVAGM
                     LLYMLW"
     gene            1420..4017
                     /locus_tag="PK196_05625"
     CDS             1420..4017
                     /locus_tag="PK196_05625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HON40931.1"
                     /translation="MSPSHQLPKNYDIEEVERRWQSTWRDEDNYFDPSSPEPRFIIDT
                     PPPYPTGNFHIGNALNWCYIDFIARYKRMRGYNVMFPQGWDCHGLPTEVKVEETHGIT
                     KNDVSRETFREMCRDLTLGNIEKMRATMRRLGFSIDWSNEYITMLPDYYRKTQASFLQ
                     MLKAGDIYQSEHPVNFCSRCETAIAFAEVNYASRTTQLNYFDFDGIEIATTRPELLAA
                     CVAVAVHPEDERYAWLRGKKLSVPIFGHDVPVIEDPAVDPSFGSGAVMVCTFGDKTDV
                     YWWKEHNLDLRKAIDRKGVMTEIAGPYAGMTSEACREAILADMKRAGILKRQEEIEQR
                     VGTCWRCKTPIEILSERQWFVRVHHDDVLEAARKITWIPEHMFTRMENWVNSMEWDWC
                     ISRQRIFATPIPVWFCTTCGGMVLPAEEELPIDPTVDKPRHPCPNCGGNEFAGEKDVL
                     DTWMDSSISVLHVTGWDGSNKKPPYFPAQLRPQGHDIIRTWAFYTILRAKALVGSHPW
                     EQILINGMVLGEDGFKMSKSRSNIISPETIIDQYGADAFRQWGAGGAATGSDIMFNWN
                     DVVAASRFQTKLWNIFRFVMGHLERGADPAAPVTELSDRWLLVRLSGTVAEVTAAMEG
                     YQFDRALRAVREFTWNTLADDYIEIVKGRLYEDDEEGRASACSALRITMDVLCRLLAP
                     IIPHFSEECYHTLTGRSVHKETWPEFEYTDEDAQRKGNLLVKTVAELRRYKHDHGMAL
                     NAPLGHVTIYTPEPLDDAGDAGRALVADLHWVTGTPQLEEVVTGVEFNMAVIGPALRG
                     LARGFMEAVKALPPEALKNPPATVTVDGKEIPVPPGAFTPKVTYRVGGEDVDLLTPGD
                     LVVTIGRKP"
     gene            complement(3974..4591)
                     /gene="tmk"
                     /locus_tag="PK196_05630"
     CDS             complement(3974..4591)
                     /gene="tmk"
                     /locus_tag="PK196_05630"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HON40932.1"
                     /translation="MLITVEGIDGSGKTTLLARLRGLLADLDPIFTREPGATWVGDAV
                     RRAIAERMDPITEALLFCADHAAHIDTLIRPALDEGRIVISDRYADSRFAYQPVVLEG
                     VLPEPREWLRRIHEGWSIRPDRTFLLVLPVDDAISRLDPETKREYFENAGILERVQEN
                     YLEIAASDPARFVIVDALLEKETVARFVADEIRAFARSSRRDPRA"
     gene            complement(4585..5265)
                     /locus_tag="PK196_05635"
     CDS             complement(4585..5265)
                     /locus_tag="PK196_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HON40933.1"
                     /translation="MELILALDLAGGVVVHGKAGNRSAYRPLTWGLAPSAEPGAYLSA
                     LSPRYLYIADLDSIQGGRPQDGLVRRCAAMVERCYLDRGCRSPADSAAVDGVIPVVGT
                     ETAAAAIDDLAAYPGGYLSIDIRNGRVLPWGITPAEMLRFASELAFEGCIVLNIGAVG
                     TERGLLRGGLEEMRGCYPGRLLYGGGVSGVDDIHLLADTGFDGAIVATAVHRGTMPLE
                     WIRRGQPC"
     gene            5310..5999
                     /locus_tag="PK196_05640"
     CDS             5310..5999
                     /locus_tag="PK196_05640"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HON40934.1"
                     /translation="MQLLVSPSSIEEAKASLPADIIDVKRPAEGSLGANFPWIIREIK
                     EIAGSKPVSAAIGDNEYKPGTAALSAYGAAHAGADYIKVGLMFDGVDRAREVVEAVTT
                     AVKREFPDRYVVIAAYADFNRLGTISPFSITPLIADAGADVAMIDTGIKDGKGLFAFM
                     DEETLTLFVRWNRELGLRTALAGSLRFEDLDALKRINPDIIGVRGMVCGGDRSSTIRA
                     ELVERAKMMIG"
     gene            6003..7469
                     /gene="guaB"
                     /locus_tag="PK196_05645"
     CDS             6003..7469
                     /gene="guaB"
                     /locus_tag="PK196_05645"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HON40935.1"
                     /translation="MYIEKLKMETTFTFDDVLLEPAESWVEPDEADVRSRFSRNIPLN
                     IPLVSAAMDTVTESAMAIAIAREGGIGVIHRNMTPESEAAEVRVVKQAEDLIEREVVA
                     VGPDTTVTEVERIMRQYGIGGVPVIENDRVIGIVSRRDIRAILPKHGDAKISDYMTKK
                     LITAPEDITAENALETMYANKVERLPVVDGDRRLVGIITMRDILEKRQYPRATRDSNG
                     KLRVAAAVGPFDFNRAVMLADAGADTLVVDCAHGHNMNVVRAVGEIKASVTVDVVAGN
                     IATKQAALALLDTVDGLKVGIGPGSICTTRIVAGVGVPQISAIANVAEAAGEADVPVI
                     ADGGIRYSGDIAKAIAAGADCIMAGSLFAGTDEAPGRITTIKGRRYKQYRGMGSLGVM
                     SSGESSDRYFQKKEIGRTKFVPEGVEGVTPYVGRVSDVIYQLVGGLKSAMGYTGSKTV
                     ADLKRNGRFIRITPAGYGESHPHNIMITDEAPNYRLFE"
     gene            7507..8124
                     /locus_tag="PK196_05650"
     CDS             7507..8124
                     /locus_tag="PK196_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HON40936.1"
                     /translation="MLERGDVSRLLDILGNRNRRRIIELLRQKPCFVTEISERLLLSP
                     KAVIEHLQLMEREQILGSYQDERRRKYYYLSHDINLIINLQKQDDAVLPGVEEDRQAR
                     ITRDLVLLRRMIRVHDELLENLEHLEDEIESRFTDLMREGIFTDEGELKIILALVQSD
                     LSAGELKDVCGLSADELKKRLGSLVKMGIVEKTNNRYRISVTHGG"
     gene            8114..8533
                     /locus_tag="PK196_05655"
     CDS             8114..8533
                     /locus_tag="PK196_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40937.1"
                     /translation="MADNPYDEMFRKIERLMERILSDLPDCDPKIIGFAIISGPTGEG
                     FYPCISDDNYDDPEVEVIEGDDCIYITTEVNVLADGAPYVTFHKKSVTLCTGGEREMI
                     VDLNCEIDLVHSSYNVQHGVIDVVCRKREMPGEIRRS"
     gene            complement(8599..8940)
                     /locus_tag="PK196_05660"
     CDS             complement(8599..8940)
                     /locus_tag="PK196_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HON40938.1"
                     /translation="MSDLEEEALSVIQSHREGVLQSDLWKLLDIDSRKCSRIVKRLLD
                     AGLIERIEFRSDGIKTYLLRAKKRAVDPGLILAGEEVLPCIGCDRECDPEVCTILLDW
                     IYQLALEGYQE"
     gene            complement(8946..9182)
                     /locus_tag="PK196_05665"
     CDS             complement(8946..9182)
                     /locus_tag="PK196_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoprotein"
                     /protein_id="HON40939.1"
                     /translation="MVNGIVLPVKKVFSLVDSKIVVEIKDDGRKLKGRLVAVDEHLNL
                     HMDETTEYTGDQRGRTLGTVVIRGNNILTIAPLL"
BASE COUNT         2109 a         2637 c         2739 g         1700 t
ORIGIN      
        1 acacttaaga atctcttccc ggttcgggat ccggcataga cctcccctga ctggaactta
       61 ccacagcata tatagaagca tccccgccac cccgccaaag aacgtggcgg tcaggtttgt
      121 gccggagttg ccgatcctgc cgctgttctc gagcgttgcc ccaaccacgc tgtcgacatt
      181 ggtgccgatg aaacccgcaa tgaccgtcac aatggcaagt ttcgggtcag caacgcccat
      241 cacaagggca acggcagcaa caatgatcga tgccaggatg gccgcaactt cgccgcgcag
      301 tgtcaccccc ccgtttgtac cccgtggtac gggtttgaag gtggtgatca ggtatggcgt
      361 ctttcccgtg acaccaatct cgcttgccgc ggtatcggca gcggccgagg caacactact
      421 catgaatagt gccgcaaacg ccggattccc ggttataccg tagaggatgg cgccgcctgt
      481 tgccacaaga ccgtttgcaa agacgttgaa gtaaccgcgc accccgccgt gctcctgcgc
      541 gacgccgaac cgttccttgt cagcatagcg gtaacgggtt gccgctgcac cgatgatgaa
      601 gaatgtgagc atgatgagga accacctgac gtccgcaaat actatcagta tgatcccgat
      661 catcgccccg gagaataggc cgctgatgtc ggcaaccctc agacggtagg aggtgtatcc
      721 gaacccgaag gctatcacgg ccgcagcgat gaggatgctc aggtcgacct catacttgag
      781 ctcctcaaag aggaacatgg tcatcgcgac gccgagcgcc tccaccatca gcgcatcgtc
      841 ccgcccgccg agaaccgccc tgaggaggac cgcagcaacc acccccatca gggcgacaag
      901 ggccacggtg tagtggaggt aaaacatcac cgcaatcgac acaacgattg cagcaagcat
      961 gtggtagaga tacgaggcat gctcccctcc ggtgaggcgg aacgccacct ctccgaagac
     1021 aacgatgccg agcgtgcagg taaaaacaaa cgtcgagagc cagccaagcc cgtagagcac
     1081 ggcaagtgcg attatagata gagatacgta gcgggtatcc ctgatgagag agagaagaag
     1141 cgagaataaa acgaggaata acgtgagcag ccagggcggc tggagcagag gggcaaggcc
     1201 tatgaagacg agggtgagca ccgatgaaag aaccagcccc tgccactttg tcattcttat
     1261 atggttgaaa ctacatgaac aaagccttta gggtcgacac ccggtgtatc ccctgcattt
     1321 tgcccctaac ccctcctgaa tgcaggatcc gcaggcggcc ggaggaaagt cacgtcgaac
     1381 attatatttc tgagggaaga gagagattat acgctgaaaa tgtcgccgtc acatcaacta
     1441 cccaaaaact acgatatcga agaggtggag aggcgctggc agtcaacctg gcgggatgaa
     1501 gacaactatt tcgacccctc ctcgccagaa cccaggttta tcatcgacac tccaccgcct
     1561 tacccaaccg gcaacttcca tatcggcaac gcattaaact ggtgttatat cgacttcatc
     1621 gcgcgctaca aacggatgcg cggctacaac gtaatgttcc cgcaggggtg ggactgccac
     1681 ggcctgccca ccgaggtgaa ggtcgaagag acgcacggga tcaccaaaaa cgacgtatcc
     1741 cgtgagacgt tccgggagat gtgccgcgac ctcaccctcg ggaacatcga gaagatgcgg
     1801 gcaaccatgc ggcgtctcgg gttctccatc gactggagca acgagtacat caccatgctc
     1861 ccggattatt acagaaagac ccaggcgtcg ttcctgcaga tgctcaaagc cggcgacatc
     1921 taccagagcg agcatccagt taacttctgc agccgctgcg agacggccat cgcgttcgcc
     1981 gaggtaaact atgcatcccg caccacccaa ctcaactact ttgacttcga cggcatcgag
     2041 atagccacca cccggccgga actgctcgcg gcctgcgttg cggtggccgt ccatccagaa
     2101 gacgagcgtt acgcctggct tcgggggaag aaactcagcg tcccgatctt cggccacgac
     2161 gtcccggtga tcgaggatcc cgctgtcgat ccctcgtttg gcagcggggc ggtgatggtg
     2221 tgcacattcg gcgataagac cgacgtctac tggtggaaag agcataacct cgacctccgt
     2281 aaagcaatcg accggaaagg ggtcatgacc gagatcgcag ggccgtatgc aggcatgaca
     2341 tcggaggcct gcagggaggc aatccttgcc gacatgaaga gagccgggat cctgaaacgg
     2401 caggaagaga tcgagcagcg tgtcgggaca tgctggcgct gcaagacccc gatcgagatc
     2461 ctctccgagc ggcagtggtt tgtccgcgtc caccatgacg atgttcttga agcggcgcgg
     2521 aagataacct ggatcccgga gcacatgttc acgcgtatgg agaactgggt aaactccatg
     2581 gaatgggact ggtgcatctc ccggcagcgg atattcgcga cccccatccc ggtctggttc
     2641 tgcaccacct gcggcgggat ggttcttcca gccgaagagg aactcccgat cgacccgacg
     2701 gtcgacaaac cccggcaccc ctgcccgaac tgcggcggga acgagttcgc cggcgagaag
     2761 gacgtgctcg atacctggat ggattcatct atatcagtgc tccacgtcac cgggtgggac
     2821 gggtccaaca aaaaacctcc atacttcccg gcgcaactgc gcccccaggg ccacgatatc
     2881 atacggacat gggcatttta caccatcctc cgggcgaagg cgctggtcgg gagccacccc
     2941 tgggagcaga tcctcataaa cggaatggtg ctcggtgaag acggtttcaa gatgagcaag
     3001 agccgcagca acataatctc ccccgagacg atcatcgacc aatacggcgc ggacgccttc
     3061 cgacaatggg gagctggcgg cgcggcgacc ggttcagaca tcatgttcaa ctggaacgat
     3121 gtcgtcgccg catccaggtt ccagaccaaa ctctggaaca tcttccggtt tgtcatggga
     3181 caccttgaac gcggcgccga ccctgcagcg ccggtgaccg aactctccga ccgctggctg
     3241 ctcgtccggc tctccgggac ggtcgcggag gtcaccgctg caatggaggg ctaccagttc
     3301 gaccgggcgc tcagggctgt acgggagttc acctggaaca cccttgccga cgactacatc
     3361 gagatcgtga aaggccgtct ttatgaggat gatgaagagg gaagagcaag cgcctgcagt
     3421 gcgctcagga ttaccatgga cgttctctgc cgcctcctcg cccccataat acctcacttc
     3481 tccgaagagt gctaccatac cctcaccggg aggagcgtgc ataaagagac ctggccggag
     3541 tttgaataca ccgatgaaga tgctcagcgc aagggcaacc tcctggtgaa gactgttgcg
     3601 gaactgcggc gctacaagca cgaccacggc atggccctga acgcccccct cggccacgtg
     3661 acaatctaca cgccggaacc gctcgacgat gccggtgatg ccggacgggc gctcgtcgcc
     3721 gatctacact gggtaaccgg gacgccgcaa ctcgaggagg tcgtgaccgg cgtcgagttc
     3781 aacatggcgg tcatcgggcc ggccctccgc ggtctcgcca gagggttcat ggaggccgtt
     3841 aaggctcttc caccggaggc tctcaaaaac ccgcctgcaa ccgtcacggt cgatggcaaa
     3901 gagatcccgg tgccacccgg cgcgttcacg ccgaaggtca cctaccgggt ggggggagag
     3961 gatgtggatc tcctcacgcc cggggatctc gtcgtgacga tcgggcgaaa gccctgatct
     4021 cgtctgcgac aaacctggca accgtctctt tttcaagcag cgcgtcgact atcacaaacc
     4081 gcgcagggtc agatgcggcg atctccagat agttctcctg cacacgttca aggatcccgg
     4141 cgttctcaaa gtattctctc ttcgtttcag gatcgagcct tgagatggca tcatccacgg
     4201 ggagtaccag caggaacgtc cggtcaggcc ggatcgacca cccctcgtgg atccggcgca
     4261 accactcccg tggctcgggc agcacacctt ccaggacgac cggctggtag gcaaacctgg
     4321 agtcggcgta acggtctgag atgacgatcc tcccctcatc gagcgcaggc cggatcagcg
     4381 tatcgatatg cgcggcatgg tcggcgcaga agagcagcgc ctcggtgata gggtccatcc
     4441 gctcggcgat cgcccgccgc acagcatcac cgacccaggt tgcaccgggc tcacgggtga
     4501 agatgggatc caggtcagcc aggaggccgc ggagacgggc aaggagtgtg gtcttcccgc
     4561 tgccatcgat cccctcgacg gtgatcagca gggctgcccc ctccggatcc actcaagcgg
     4621 catcgtgccg cgatggacgg cggtcgcgac tatagccccg tcaaacccgg tatcggcaag
     4681 gagatggatg tcatcgaccc cggagactcc gccgccgtag aggaggcggc cggggtagca
     4741 tccccgcatc tcctcaaggc ccccccggag gaggccgcgt tccgtcccca cggcaccgat
     4801 gttcaggacg atgcagccct caaacgctaa ctccgatgcg aaacgcagca tctccgccgg
     4861 agttattccc caggggagca cccggccgtt cctgatgtcg atgctgagat agccgccagg
     4921 gtaggccgca aggtcatcga tggccgctgc cgctgtctcg gtgccgacga ccggtatcac
     4981 accatcgacc gccgcggagt ctgcgggcga ccggcaaccg cggtccaggt aacaccgctc
     5041 gaccattgca gcacagcgcc tgaccaggcc gtcctgcggt ctcccgccct ggatactgtc
     5101 caggtcggcg atgtagaggt accggggcga aagagcggag aggtaggccc cgggttcggc
     5161 cgatggcgca agcccccagg tcagcggccg gtatgcagaa cggttgcctg ccttaccatg
     5221 caccacaaca cccccggcaa gatccaatgc aagaatcagt tccatgtcct caacgtaatc
     5281 actattaggg tgaaggatca ttaatttcta tgcaattact cgtcagccca agcagcattg
     5341 aagaggcgaa agcctcgctt cccgctgaca taatagacgt taaaagacct gctgaaggct
     5401 ctctgggcgc taattttcca tggatcatac gtgagatcaa ggagatcgcc ggcagtaagc
     5461 ccgtcagtgc tgcaataggg gacaatgagt ataaacctgg aaccgcagcc ctttcggcat
     5521 acggtgccgc acacgccggc gccgattaca tcaaggtcgg cctgatgttc gacggcgttg
     5581 atcgtgcccg tgaggtcgtc gaggcggtaa ccaccgcggt gaaacgggag tttcccgata
     5641 gatatgttgt catcgcggcg tatgcagatt ttaacaggct tggcacgatc tcgccgtttt
     5701 cgatcactcc gctgatcgcg gatgcagggg ctgacgtggc catgatcgac acgggtatta
     5761 aagacggaaa ggggctgttt gcgttcatgg acgaggagac gcttaccctg tttgtcaggt
     5821 ggaaccgtga actcgggttg cggaccgccc ttgccggttc gctgaggttt gaagaccttg
     5881 atgccctgaa acggatcaac ccggatatca tcggtgtccg ggggatggtc tgcggtgggg
     5941 atcggtcgag cacgatcagg gcggaactgg ttgagagagc aaagatgatg attgggtgat
     6001 gtatgtacat cgagaaactg aagatggaaa caacatttac attcgatgac gtgctgctcg
     6061 agcccgcgga gtcgtgggtc gagcccgacg aagccgatgt caggtcacgg ttctcgcgga
     6121 acattcctct taatatacct ctcgtgagcg ctgccatgga tacggttacc gagtcggcga
     6181 tggcgatcgc catcgcccgg gaagggggta ttggcgtcat ccaccggaac atgaccccgg
     6241 agagcgaggc tgctgaggtc agggtagtca aacaggcaga ggaccttatc gagcgcgagg
     6301 tggtggcggt cggcccggat accacggtca ccgaggtgga gcggatcatg cggcagtacg
     6361 gcatcggcgg tgtccctgtc atcgagaacg acagggtgat cggtatcgtc agccgcaggg
     6421 atataagggc gatccttccc aaacacggcg atgcgaagat cagcgactac atgacgaaga
     6481 agttgatcac cgcaccagag gatatcaccg cggagaacgc cctcgagacg atgtatgcaa
     6541 acaaggtcga gcgtctcccc gtcgttgatg gggataggcg cctggtcggg atcataacga
     6601 tgcgggacat actggagaag aggcagtacc cccgcgcaac ccgcgattcg aatggtaaac
     6661 tccgtgtcgc cgccgccgta gggccatttg atttcaatcg tgctgtgatg ctggccgatg
     6721 cgggtgccga caccctggtg gtggactgtg cccatggcca caacatgaac gtcgtcaggg
     6781 cggtcggcga gatcaaggcg agcgtcacag tggatgtggt tgccggaaac atcgcaacaa
     6841 aacaggcggc cctggccctt ctggataccg tcgacgggct gaaggtaggg atagggccag
     6901 gctcaatctg cacaaccagg atcgttgcgg gggtgggcgt accgcagatc tcggcgatag
     6961 caaacgttgc ggaggcggcg ggagaggccg atgtgccggt catcgccgat ggcggcatcc
     7021 gctactccgg ggatatcgca aaggcgatcg ccgcgggtgc ggactgtatc atggcaggca
     7081 gcctctttgc cggaacggac gaggcgccgg ggaggattac aacgatcaag ggccggcgct
     7141 acaagcagta ccgcgggatg ggatcgcttg gcgtcatgag cagcggcgag tcgagcgacc
     7201 gttacttcca gaaaaaggag attggaagga ccaaattcgt tccagagggt gtcgaaggcg
     7261 tcacccctta cgttggccgg gtctctgatg tcatctacca gcttgttggc ggcctgaagt
     7321 cggcgatggg ctacacaggc tcaaagacgg tagcggacct gaagagaaac ggtagattca
     7381 tcaggataac ccctgccggt tatggggaga gccatcccca taacatcatg atcacagatg
     7441 aggcgccgaa ttaccgtctc tttgaatgat aaatttttat gctgtgatca ctaacactaa
     7501 gttagtatgc ttgagagggg cgacgtttcc cggctcctgg atatcctggg aaaccggaat
     7561 agaagacgga taatcgaact gctgaggcag aaaccgtgtt tcgtgacaga gatctccgaa
     7621 cgcctgctgc tcagtccgaa agcggtaatt gagcatctgc aactgatgga gcgggagcag
     7681 atccttggat cataccagga tgagcgaagg cgaaagtatt actacctctc gcatgacatc
     7741 aatctcatca taaacctgca gaagcaggat gatgctgtcc tccccggggt tgaggaggac
     7801 cggcaggcac ggatcacaag agacctcgtg ctgctcagga ggatgatcag ggttcacgat
     7861 gaacttctgg aaaaccttga acacctggaa gacgagattg agtcacggtt caccgatctc
     7921 atgcgcgagg gaatcttcac agatgaagga gagttgaaga tcatcctcgc cctcgtgcag
     7981 tctgacctga gtgccgggga actcaaggat gtgtgcggcc tgtccgcgga tgagctgaaa
     8041 aaaaggctgg gcagcctcgt gaagatgggg attgtcgaga agacgaacaa ccgataccgg
     8101 ataagtgtta cacatggcgg ataaccctta cgacgagatg tttcgaaaga tcgagcgact
     8161 gatggagcgg atccttagcg atctgcccga ttgcgacccg aagatcatcg ggttcgccat
     8221 catcagcgga ccgaccggag agggttttta cccctgcatt tccgacgaca actacgatga
     8281 ccccgaggtt gaggtgatcg agggggatga ctgcatctac ataacaacgg aggtaaacgt
     8341 cctggcggac ggtgcaccct acgtcacgtt ccataaaaaa tctgtgaccc tctgcaccgg
     8401 tggtgagagg gagatgatag tggacctcaa ttgcgagata gacctggtgc acagttccta
     8461 caacgtgcag cacggtgtga tcgatgttgt ctgccggaaa agggagatgc cgggagagat
     8521 ccgcaggtct tgaaccgcgt tagtgtgggg ggcaaacccc caaaagaacc tgttttcgtg
     8581 atggcacgcg aagagtggtc actcctgata cccttcaagg gcgagctggt atatccagtc
     8641 tagaagaatg gtgcacactt ccgggtcgca ttcccggtcg cagccgatgc aggggagaac
     8701 ctcctcgccg gcaaggatga gacccgggtc aacggcgcgt ttcttcgccc gcaggagata
     8761 tgtcttgatc ccgtcactgc gaaactcgat ccgttcgatc aaaccagcat ccagcaaccg
     8821 cttcacaatc cgggagcact tcctgctgtc aatatcaagg agtttccaga gatcgctctg
     8881 gagaactcct tcacggtgcg actggatgac gctgagcgct tcttcctcca ggtcagacat
     8941 ggtaatcaga gcaggggcgc aatggtcagt atattgttgc cgcgtatgac aaccgttccg
     9001 agagtgcggc cccgctgatc tccggtgtac tctgtcgtct catccatgtg caggttcagg
     9061 tgctcgtcga ccgccacgag ccttcctttg agtttcctgc cgtcatcctt gatctcgacg
     9121 acgattttag agtccacaag cgaaaaaacc ttctttacgg ggagtacaat gccattaacc
     9181 atctg
//
LOCUS       DAOSZX010000056         9127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6476_length_9127_cov_21.698743, whole genome shotgun sequence.
ACCESSION   DAOSZX010000056 DAOSZX010000000
VERSION     DAOSZX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6476_length_9127_cov_21.698743"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..614
                     /locus_tag="PK196_05670"
     CDS             54..614
                     /locus_tag="PK196_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HON40940.1"
                     /translation="MYQGERDDRAFTGLEAAIVLIAIVVVASVFAYVVLGAGMATSQK
                     SQQTMYAALGEAASGLRTGDSVIVKLDNDRGYVRFVEFDLETVTDIAAIDPGRLRYTI
                     ATEETVVTIPPGDPRATITWRYRRDDGDLLEAGEVIAVRLNTADAAIDRGDTFSIEIT
                     AADGATISLSRRVPAGADKNVYLELF"
     gene            592..1173
                     /locus_tag="PK196_05675"
     CDS             592..1173
                     /locus_tag="PK196_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HON40941.1"
                     /translation="MSTLNYSEAGFTGLEAAIVLMAFVVVAATFAHAVLGTGLTFAGE
                     SQSVVHQSVRQTGSGLIAAGSIHGISSRPERIDTLVIPIGLTAGSEPIDINTVSIRLV
                     GSAHMEEVCRNNPLIGVFPENGHWSVGERINSDDDHLLEPGERYILYITPMNCLDCRP
                     SRTFALEIKPAGRVALRVERTVPGSITPLTRLE"
     gene            complement(1148..1828)
                     /locus_tag="PK196_05680"
     CDS             complement(1148..1828)
                     /locus_tag="PK196_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HON40942.1"
                     /translation="MFLACHLFFGLVLGLALAGRTGDRRLIGFAALGALIPDLIDKPV
                     GHILFAETLDSGRLFGHGLLFVGILLAAGIISYRRRDSFAILAVAAGVFSHQILDAMW
                     AMPVTWYFPLLGRYEPQGYVNYFGGALLAEVSSLSEWIFLAASAGIVLAVSRGLSAAV
                     IRVSAVILGLLAILSLALFAAGSPESVLMAGAGSVDYLLLAVVAAAGVIVVSRLHPGG
                     LIQDGSAG"
     gene            1924..2115
                     /locus_tag="PK196_05685"
     CDS             1924..2115
                     /locus_tag="PK196_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40943.1"
                     /translation="MEPTLRSEILILFLSFLIGSAAGWWSRIHWSGAIPTVAATLVST
                     VAAYLMITGILRAAGRYPG"
     gene            complement(2112..2813)
                     /locus_tag="PK196_05690"
     CDS             complement(2112..2813)
                     /locus_tag="PK196_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HON40944.1"
                     /translation="MEQRTGCLICGLAIVYSEEARDLACAICGEVVSTTAACEAGHFV
                     CDRCHGLEAVDLIEQFCITTELDDPIAIACILMRNPAVRMHGPEHHFLVPASLIAAYC
                     NHRGEPGRKGELLARARDRASRVPGGFCGTHGTCGAAIGTGIFVSLITGSTPLKKQEW
                     SLSNQMTARSLMAIARHGGPRCCKRNSWLAIRTAVTFLEERFSVSLPVEGAVRCGFSD
                     INRECLLKECPFYAG"
     gene            complement(2878..3105)
                     /locus_tag="PK196_05695"
     CDS             complement(2878..3105)
                     /locus_tag="PK196_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HON40945.1"
                     /translation="MKKLTRSRTNRWIAGVCGGVGEYLDIDPNIIRAVYIVFTVLTGF
                     VAGLIIYILLWIIIPEGAEASPVVEAPPVDV"
     gene            complement(3364..4584)
                     /locus_tag="PK196_05700"
     CDS             complement(3364..4584)
                     /locus_tag="PK196_05700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HON40946.1"
                     /translation="MDRLHLILERYFGYQAFYPCQREIIQDLLAGRDVLAVLATGGGK
                     SLCYQVPAVLGDGVTVVVSPLIALMKDQVDDLQARGICAAALNSSGTYAARRRTLAEL
                     EEGLLQILYVSPERAVSEVFLDLVASLPLNLIAVDEAHCISMWGHQFRPEYRALAVLK
                     ERFPRVPVVALTATATPDVRQDIVRQLRLADPAVYVGSFNRENLRYVVTPKGGDAYRR
                     LRDYLLMWQGESGIVYLATREGARELAASLRADGIPAEPYHAGMTASARAKTHDRFIG
                     GEAVVVCATSAFGMGIDRPDVRFVVHYDMPETLEAYYQESGRAGRDGKPADCILFYGE
                     GEARRLRSLVDRNLASDFQRGVARSKLQSMVDYCTSTVCRRKVILDYFGERFDAPCGG
                     CDICAPTGDRCFTQ"
     gene            complement(4653..5444)
                     /locus_tag="PK196_05705"
     CDS             complement(4653..5444)
                     /locus_tag="PK196_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HON40947.1"
                     /translation="MIDRYPFLISIPHGGTSVPPEVRGLVNLSHKEIIYNSDPQTRFI
                     YGFDASVEAVIDFEVSRVFVDTNRPPYAYPPRSQDGVVKVITQDGTPVFRNGQAPGRE
                     LISALLKNYYHPFHARLAGALENHPIGIAFDCHSMLPRSPPVQRDAGRERPIICLGNR
                     GDRNGRPGAKNRPVTCPPAWLQALARSFEEEFAGEGRVAMNDPFRGGFIAAAHHRRTR
                     VPWVQIEVNRALYETEDGLVDQERLVELRDRIFAAITRFWESVLD"
     gene            complement(5516..8686)
                     /locus_tag="PK196_05710"
     CDS             complement(5516..8686)
                     /locus_tag="PK196_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON40948.1"
                     /translation="MDLFGNILGSGKGANPAVKQGLSQFEAGNYTEAVKSFEKAFRVE
                     PDNRLVWQMMGQALACLGRDTEAAEWLRRVIEVSPDDTGMLRTLGHLLARTGEYEEAA
                     ACFARILEENPGDADASYWQAEMLERLGRYADAAAAYAEALGANPEDVLLREKCATML
                     ERSGNYRQAAISFEKVYRSNPDRSDALARAGAAFLAMGDYQSAVETFDRLLKTGQHKL
                     DALYGKARALEYLGLFQDAAGCWEEILALDPGNVAGWYQRGSVLLRAGRPAEAIECFE
                     KVTIADPDHIAARYAMGLACEALGRHDRAVKNFDQILKHDQNQVQVWYARGMALMHLS
                     RYEEAIQSFDRVLEGQASAGTRLISGGADLALFEREDLAENRKRISLKFDAPGRVLHT
                     CRGTALLHLGRYAEAEEEFDCVLESDPGDVPVMAQSGIALLHLGRYDAAVRRLETVLE
                     TEPGNTAVRSMLAEALANLGRYDEALAYLDENGNLCRKGEMLLHLARYAGAVESFDTF
                     LERCPRDPVAEIGRGEALMHLSRYDEAAVCYEAVLESDPENRAALFGHGMALFCQGRY
                     QEALESVNRVVEADAGALLLKARLQEALGRCAAAAECYETLLEAEPDAAGYQVNLGFV
                     LAALGRFEEAATWFGKAAKARPGDLFAWYGRGQALERLGRHAAAAECYITVVRERPDD
                     TAVVSALAAILAHLGRHREAIECCEKVLATEPSNLAAARLRAGILEALGEHEEAAKAY
                     ERYLDLVPGDRDARMAFGMTLERGGRFDDAIRQYALLLKKDEGDTGAWYALESALMHM
                     GRYDEALQCSEKIVEANPENVAAWQRRGEILMWLGRYADAVECLERVTAADPENLVSL
                     RRLGEAYEKAGRYQDALSVYTALLEREPASIETLYARASALVHLGRYGEAVRSIDRII
                     AIQEENPAALFMRGAVLERVGRYDEALATYEKALEIDQENAAVWMAAGILMDAVGRHN
                     DAVRAFDRAIDLGGGGVHAWLCRGIALDRLGKPDQAIACYDKVLEADPHQARAWYLKG
                     RALDRLGRFADAVACFNSALENEQDA"
BASE COUNT         1790 a         2943 c         2699 g         1695 t
ORIGIN      
        1 ggcaagttca tatcagcagc cttttatcga gttgagcgca aaattttttg tctatgtacc
       61 agggagagcg ggacgatcgg gcgtttaccg gactggaagc ggcaatcgtt ctgatcgcca
      121 tcgtcgtcgt cgcatctgtt tttgcatacg tcgttctcgg ggcgggcatg gccacatcgc
      181 agaagagcca gcagacaatg tatgccgctc tcggtgaggc cgcctcgggc ctgcgcacgg
      241 gagactctgt catcgtcaaa ctggacaatg atcggggtta tgtcaggttt gtcgagttcg
      301 atcttgagac cgtgaccgat atcgccgcaa tcgatccagg gagactgaga tacaccatag
      361 caacagagga gaccgtggtc acgattcctc ccggtgatcc ccgtgcaacc ataacctggc
      421 gctaccggag agacgatgga gaccttcttg aggctggaga ggttatcgcg gtgagactga
      481 acacggctga tgctgccatc gaccgcggcg atacgttcag catcgagata accgccgcag
      541 atggagcgac catttcgctg agccggagag ttccagcggg tgctgacaag aatgtctacc
      601 ttgaactatt ctgaggccgg attcacaggg cttgaggctg ccatagtgct catggcgttt
      661 gtcgtcgttg cagcaacctt tgctcatgct gtccttggaa caggtctcac ctttgccggg
      721 gagagccagt ctgtcgtcca ccagagcgtc cggcagacgg ggtcaggcct cattgcggca
      781 ggttccatcc acggtatcag cagcagacca gagaggatcg ataccctcgt catccccata
      841 ggtctcaccg caggttcgga accgatcgat atcaatacag tctcgatccg tcttgtgggt
      901 tctgcgcaca tggaagaggt ctgcaggaac aaccccctga tcggtgtttt cccagaaaat
      961 gggcactgga gcgtcgggga gcggatcaac agcgacgacg accatctcct ggagcctgga
     1021 gagcgctaca ttctctacat aacaccgatg aactgcctgg actgcagacc ctcccgaacg
     1081 tttgctctcg agatcaaacc ggcagggagg gtggcgctcc gggtcgagcg gacggtgccg
     1141 ggcagcatca ccccgctgac ccgtcttgaa tgagaccacc aggatggagc cgggagacca
     1201 ctatcacacc ggctgccgcc accaccgcaa gcagcaggta gtccacagac cccgccccgg
     1261 ccatcaggac gctctcgggg ctgcctgcgg caaagagagc gagtgagagg atggcaagga
     1321 gtccaagaat cacggcggat acgcggatga ctgcagcgga gagacctctg gagactgcaa
     1381 ggactatacc tgccgatgct gccaggaata tccattccga gagcgaggag acctctgcca
     1441 ggagggcgcc gccaaagtag tttacgtatc cctggggctc gtagcgccca agcagcggaa
     1501 aataccaggt gacgggcatc gcccacatag catccaggat ctggtgcgag aacacgcctg
     1561 ccgcaaccgc caggatcgca aacgaatccc gtcttcggta acttatgatc cccgccgcga
     1621 gcaggatgcc aacgaagagc agcccgtgtc caaagagacg cccggagtca agggtctccg
     1681 caaacaggat gtggccgacc ggcttatcga taaggtcggg gattagagcc ccgagagcag
     1741 cgaagccgat gagtcgccga tcgcccgtgc gaccggcaag cgcgagaccc agcacgaggc
     1801 cgaaaaagag gtgacaggca aggaacatct ctaattaatg tgccgctgcc gggttataac
     1861 cgctgctgat gtgcgatcac aaaaacggtt aatcccttcc aggtgcgata ccttaccact
     1921 cccatggaac cgactctacg gtcagagatc ctgatcctct ttctctcctt cctgatcggc
     1981 agcgcagcgg ggtggtggag ccgtatccac tggagtggcg ccattccaac cgttgcggcg
     2041 acacttgtca gcacggttgc cgcctacctt atgatcaccg gcatcctgag ggcggcgggc
     2101 cggtatcctg gttaaccggc gtagaagggg cactccttga gcaggcactc gcggttgata
     2161 tcgctgaacc cgcaacgcac cgccccctcc accgggaggc ttacactgaa acgttcctca
     2221 aggaatgtga ccgcggtgcg gatcgcaagc catgagttgc gtttgcagca tctcggcccg
     2281 ccgtgccggg cgatcgccat caggctgcgg gctgtcatct ggtttgatag cgaccactcc
     2341 tgcttcttca acggggttga cccggtgatc aggctgacaa atatgcccgt cccgatcgcc
     2401 gcaccgcagg tgccgtgggt cccacagaaa cccccgggaa ccctgctggc tctatcgcgg
     2461 gcgcgggcga ggagttcgcc cttccgcccg ggttcaccgc ggtggttgca gtaagcggct
     2521 atgagagacg ctggcacaag aaaatggtgt tccgggccgt gcatccttac cgccgggttc
     2581 cgcatcagta tacaggcgat ggcaatcggg tcgtcaagtt cggttgtgat acagaactgc
     2641 tcgatcaggt caaccgcttc aaggccgtga cagcggtcgc atacaaagtg cccggcctcg
     2701 caggcggcgg tggtgctcac aacttcgccg cagatggcgc acgcaagatc gcgtgcctct
     2761 tcgctgtata cgattgccag accgcagatc aggcatcctg tcctctgctc catgaaagta
     2821 tggttgcgtc accacctccc caataacctt gcggccggtt cgtaaggagg tctcttccta
     2881 tacatcaacc ggaggcgctt ccaccaccgg actcgcctct gcaccctcag ggatgattat
     2941 ccagagcagg atatagatga taagccctgc aacaaacccg gtgagcacgg tgaagactat
     3001 gtagaccgcc cggatgatgt tcgggtcaat atccaggtat tctccaacgc cgccacatac
     3061 cccggcgatc caccggttgg tccttgaacg ggtcagcttc ttcatgatct ctccggagat
     3121 atctcttcac cccaaccctt aaaaaggtgt ttcctgccgg ggggggcgtc gcatcacccg
     3181 accccccctc tcttgcagcc tctggaaacg gtatatgcct tcgccgggac acccccacct
     3241 cgcggggcac cgcatctatg gagtatctgc ggcggcgatg gacgctcccc ggaatccgtc
     3301 cctgcagagg tcttgcggga tcatcagaac ccgggcgcag ccagatggag ccggtttgac
     3361 aggttattgc gtgaaacaac gatcaccggt cggagcgcag atgtcgcagc ctccgcaggg
     3421 cgcatcgaac cgctcaccaa agtagtccag gataactttc ctccggcaga ccgtgctggt
     3481 gcagtaatcc accatgctct gcagcttaga acgtgcgacc ccgcgctgga agtcagaagc
     3541 caggttgcgg tcgacgagcg acctgaggcg gcgtgcctcc ccttcgccgt agaagaggat
     3601 gcagtcagcc ggcttaccgt ccctcccggc ccggccgctc tcctggtagt aggcctcaag
     3661 cgtctccggc atatcgtagt ggacgacaaa ccgcacatcc ggtctgtcta tgcccatgcc
     3721 aaacgcgctt gtcgcgcaga cgaccaccgc ctccccgccg atgaaccggt catgcgtctt
     3781 cgcacgggcg gacgctgtca tcccggcgtg atacggttcc gccgggatac cgtcggcacg
     3841 gaggctggcc gcgagttccc gcgccccctc gcgggttgcc aggtatacta ttccgctttc
     3901 cccctgccac ataagcaggt agtcgcggag ccgtctgtaa gcatccccac ccttcggggt
     3961 gacaacgtaa cggaggttct cgcggttaaa actcccaaca tagacggcgg ggtccgccag
     4021 ccttagctgg cgcacgatat cctgacggac atccggcgtt gcggtcgccg tcagggcaac
     4081 caccggcacg cgggggaaac gctccttaag gacggcaagc gcccggtact ccgggcggaa
     4141 ctggtgcccc cacatcgata tgcagtgcgc ctcatcgacc gcgatcaggt tgagtggaag
     4201 agatgcgacg agatcgagaa aaacctcgct gaccgctctc tccggcgata cgtagaggat
     4261 ctggagaagc ccctcctcga gttcggcaag cgtccgccgc ctcgccgcgt aggtgccaga
     4321 ggagttcagg gctgccgcac agatcccccg ggcctggagg tcgtcgacct gatccttcat
     4381 cagggcgatg agaggagaga ccaccacggt taccccgtcc ccgagaactg cagggacctg
     4441 gtagcagagc gatttgccgc ctccggtcgc gagcacagcc agaacgtcgc gccccgcaag
     4501 gagatcctgt atgatctcgc gctggcaggg gtaaaacgcc tgatacccga agtagcgttc
     4561 caggatcagg tgcagccgat ccatcaaccc gtagatgggc gctcaaccgg catagcatct
     4621 tccccggaga cccagagcgg ggtgaagggg catcagtcca ggacgctctc ccagaaccgc
     4681 gttatggcgg caaagatccg atcccggagt tcaacgagcc tctcctgatc aaccaagccg
     4741 tcctccgtct cgtagagtgc acgattcacc tcgatctgaa cccaggggac gcgcgtccgc
     4801 cggtggtgtg cagccgctat gaacccaccc cggaacggat cgttcatcgc gaccctgccc
     4861 tctcccgcaa actcttcctc aaacgaccgg gcaagcgcct ggagccacgc cgggggacag
     4921 gtcacaggcc ggttcttcgc ccccggcctc ccgttccggt cgccgcggtt gccaaggcag
     4981 atgatcgggc gttcacgtcc ggcgtcccgc tgaaccggcg gcgatctcgg gagcatgctg
     5041 tggcagtcaa aggcaatccc tatggggtgg ttctcgagcg ccccggccag tcttgcatgg
     5101 aaggggtggt aatagttctt caggagggca gaaatcaact ccctccccgg tgcctggcca
     5161 ttccgaaaga ccggcgtccc gtcctgggtg atgaccttga caacgccgtc ctggctccgc
     5221 ggcgggtaag cgtaaggcgg acggttggtg tcgacgaaga cccgggagac ctcaaaatcg
     5281 atcaccgcct caacggaggc gtcgaacccg tagatgaaac gagtttgggg gtcgctgttg
     5341 tagataatct ccttatggct gaggttgact agaccgcgga cctccggcgg aacgctggtg
     5401 cccccgtgcg ggatcgatat aaggaaggga taccggtcga tcatctgtcc tgcaggccat
     5461 attgttcgct catcacaaaa gtgcagcggt cgggaggggc gggaaaaccc gccactcacg
     5521 catcctgctc gttttcaagc gcagagttaa aacacgccac cgcatcggca aacctgccca
     5581 ggcggtcgag cgcccggcct ttgaggtacc acgcccgggc ctggtgcgga tcagcctcaa
     5641 gaaccttatc gtagcaggcg atggcctgat cgggtttacc caggcggtcg agggcgatac
     5701 ccctgcagag ccaggcgtgg acgccgccac cgccgaggtc gatcgcccgg tcaaatgccc
     5761 tgacggcatc attgtgccgc cccactgcat ccatgaggat cccggcagcc atccagacgg
     5821 ccgcgttctc ctggtcgatc tcgagcgctt tttcgtaggt tgcaagagcc tcgtcatacc
     5881 tcccgaccct ctcaagcacc gccccccgca tgaagagcgc ggcagggttc tcctcctgga
     5941 ttgcgattat cctgtcaatc gacctgaccg cctcgccgta acgcccgaga tggacgaggg
     6001 cggaggcgcg ggcatacagt gtctcgatgc tggcaggttc cctctccagc aacgccgtgt
     6061 agaccgaaag agcgtcctgg tatctgccgg ccttctcgta agcctcgccg agtcggcgca
     6121 ggcttacgag gttttccgga tcggccgcgg tcaccctctc gaggcactcc accgcatcgg
     6181 cataacgacc gagccacatc aggatctccc cccgccgttg ccaggcggcc acgttctcgg
     6241 ggttcgcctc gacgatcttt tcagagcact ggagcgcctc atcatacctg cccatgtgca
     6301 tcagagcgct ctccagggca taccacgccc ccgtatcgcc ttcgtctttc ttgagcagaa
     6361 gcgcatactg ccggatcgcg tcatcaaacc tgccgccgcg ctcaagcgtc atcccgaacg
     6421 ccatcctggc gtcgcggtca ccgggaacga ggtcaaggta acgctcgtag gccttcgccg
     6481 cctcctcgtg ctctccgagg gcctctaaga tcccggccct gagacgggcg gccgcaagat
     6541 ttgagggttc ggtggcgaga accttctcac agcactcgat cgcctcccgg tgcctgccga
     6601 gatgtgcgag aatggcggca agcgcggaga cgacagcggt atcatccggc cgctcgcgga
     6661 cgaccgtgat gtagcattcc gcagccgcgg cgtggcgtcc cagcctctcc agggcctgcc
     6721 ccctgccgta ccaggcaaag agatccccgg gcctggcttt tgcggccttc ccaaaccacg
     6781 tggcggcctc ctcgaagcgc ccgagcgcgg caagaacgaa ccccaggttc acctggtagc
     6841 ccgcggcgtc aggttcggcc tcaagaagcg tctcgtagca ttccgcagcc gcggcgcagc
     6901 gccccagagc ctcctggagc ctggctttca gcaggagcgc tccggcatcg gcctccacca
     6961 cacggttgac tgactcaagc gcctcctggt agcgcccctg acagaagagc gccatgccat
     7021 gcccaaacag cgccgcccgg ttctcagggt cggattccag caccgcctca taacacacgg
     7081 cagcctcatc gtagcgggag agatgcatca gcgcctcgcc cctgccgatc tccgccaccg
     7141 gatcgcgggg gcacctctca aggaaggtat caaaagattc caccgccccg gcataccgcg
     7201 caagatgcag cagcatctcg cccttgcggc agaggtttcc gttctcgtcc aggtacgcaa
     7261 gcgcctcatc atacctcccc aggttggcga gcgcttctgc cagcatcgac cggacggcag
     7321 tgttgccggg ctcggtctca agaacagtct ccaggcggcg gaccgctgcg tcatacctgc
     7381 cgaggtggag gagcgcgatg ccgctctgcg ccatgacggg aacgtcgccg ggatcggact
     7441 caagaacgca gtcaaattcc tcctctgcct cagcgtagcg gcctaggtgc agcagcgccg
     7501 tcccccggca ggtgtggaga acgcgacccg gtgcatcgaa cttcagggag atgcgttttc
     7561 ggttctcggc caggtcttcg cgctcaaaga gcgcaaggtc ggcgcctccc gagatcaacc
     7621 tcgtcccggc agacgcctgg ccctcgagca cgcggtcaaa cgactgtatg gcctcctcgt
     7681 agcgggagag gtgcatgagc gccataccgc gggcgtacca tacctgaacc tggttctggt
     7741 cgtgcttgag gatctggtcg aagttcttga ccgcgcggtc gtggcggccg agcgcctcac
     7801 aggccagccc catggcgtaa cgggccgcta tatggtctgg atcggcgatg gtcaccttct
     7861 cgaagcactc gattgcttct gccggtcttc ctgcgcggag gagaaccgag ccgcgttgat
     7921 accagccggc gacgttcccg ggatcgaggg ccaggatctc ttcccagcag ccggcggcat
     7981 cctggaatag ccccaggtac tcaagcgccc gggccttgcc gtagagtgcg tcgagtttat
     8041 gctgccctgt tttgagcaga cggtcaaaag tctccaccgc gctttgataa tcccccatgg
     8101 caaggaacgc agcccccgca cgcgccagag cgtcgctgcg gtcagggttt gaccggtaga
     8161 ccttctcaaa ggagatcgcc gcctgccggt agttcccgga ccgctcgagc atcgtcgcgc
     8221 acttctcccg caggagaacg tcttccgggt ttgcacccag cgcctctgcg tatgctgctg
     8281 ccgcatcggc ataccggccg agcctctcga gcatctctgc ctgccagtat gaggcgtcgg
     8341 cgtctccagg gttctcctcg aggatccgtg caaaacaggc ggccgcctcc tcatactcgc
     8401 cggtgcgtgc aaggagatgg ccgagcgtgc gcagcatccc ggtatcgtcc ggagatacct
     8461 cgatgaccct tcgaagccac tccgcggcct cggtatctct gccaaggcag gcaagcgcct
     8521 ggcccatcat ctgccagacc aggcggttat cgggctcaac cctgaacgcc ttctcgaaac
     8581 ttttcaccgc ctcggtgtag ttcccggcct caaactgcga gaggccctgt tttaccgcgg
     8641 ggtttgcacc cttcccggaa ccaaggatat taccgaagag atccatcagg catcatccga
     8701 tgctctatct tttgcatgga gagtattcac aactttggat catccgccgg ccgcgatttg
     8761 accccttaaa atcggtgatc tgaggtataa aaaatgcagc attcgcccgg tgaacgtctt
     8821 ttatcgacca cgcgctcaga gcccggcatc ccggttgaaa cgatcctcgg cgatacggat
     8881 tccgccggcg ggatctctca tcatcgtgag gcggatcaaa cccgggcaca gcccccggaa
     8941 aactggtctg atggccgtgg ttttagcggc tccgctcttg gagggatggt tcactgcagt
     9001 ctggacggca gaggttttgt ggctgctccc cctcctctcg tcgggggcat cctgctttct
     9061 gttcttcgta aaaaaagatg ctgcagcaga ccgtaaggcc gcaccgacct cgggcggcga
     9121 ggtcgcg
//
LOCUS       DAOSZX010000057         8886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6692_length_8886_cov_20.864908, whole genome shotgun sequence.
ACCESSION   DAOSZX010000057 DAOSZX010000000
VERSION     DAOSZX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_8886_cov_20.864908"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..989
                     /locus_tag="PK196_05715"
     CDS             237..989
                     /locus_tag="PK196_05715"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HON40949.1"
                     /translation="MEIQVFGVRGLPLIRQGDDLPGMICERVAFEDGDILTVASSVYS
                     KAKGFTRDLESIVPGADAVRIAGKTGEDPRFVQAVLDSSAAILLEDPFILSELPFGHI
                     GVRAGVDHSNIEDGRVIILPPDPMAAAAEMRDAIHRISGKDVRVIITDTCGRAFRRGQ
                     TGIAIGWAGMTAIDDYRGDTDLFGHVLKITEEAVVDEIAAFANFVMGESHQGVPAVVF
                     RNCRTWKGHDAVYFTPEEDIIRAALLKEHKKD"
     gene            complement(986..1144)
                     /locus_tag="PK196_05720"
     CDS             complement(986..1144)
                     /locus_tag="PK196_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HON40950.1"
                     /translation="MAKKSGGRLVSSAGLVNYYDSDDHRAIHISPTAVLVIALATGVA
                     VYILNLLF"
     gene            1190..2236
                     /locus_tag="PK196_05725"
     CDS             1190..2236
                     /locus_tag="PK196_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON40951.1"
                     /translation="MRQPEDCVITGRALLGEELLEEDVSITVSGGIVTSIEPVSRTPG
                     LWIVPAFFNAHTHLGDTVAMDIPARGSLAGLVKPPNGLKHRILAATPRADLVRGMRAS
                     IITMMATGTAGFADFREGGADGVAALHEAAAGLDCSPVILGRDGGETVGDGAGISSVH
                     DVKNAEEIVRCARAAGKLVAFHAGEKSPDDIDEALAFEPDLLVHCTHATDAQLRRIVD
                     MGIPVAVCPRSNWVLGVAASPAHPPIARIIELGGTLYLGTDNVMFVQPDMLQEMAFTA
                     TVYRASPEEILRAAIAGARLAGVSGYLEAGQKARFIVVNPLKSNLSFSRDIRATIVKR
                     LDSSSIGQNILTLQ"
     gene            2273..2701
                     /locus_tag="PK196_05730"
     CDS             2273..2701
                     /locus_tag="PK196_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HON40952.1"
                     /translation="MFRKILVAIDGSEQADRAFEAALAEAGVWKAEAHVVYVVESGLF
                     SSLPMDSTLEIIYSVLHKEGEEILESARKKAETAGIPLTTHLCQGHVGSQIVSMAKEL
                     GADLIVLGSYGKSGVDRLLLGSATDYVVRNSPVTTMVVRS"
     gene            2734..3576
                     /locus_tag="PK196_05735"
     CDS             2734..3576
                     /locus_tag="PK196_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON40953.1"
                     /translation="MVTDVVCVEIPGNRDDVLRILKRTGISGVPVLKEGELVGIITRK
                     DLLRKAEETQLGLLMTPDPVVIRPDAPISEAAQLLVRHNIRRLPVIEDGALVGILSVA
                     DLINAIAQMRITIPIKDNFVSKTYALWEETPLTLVGRIMEISGYDAIPILMEDGTLSG
                     IISERDLIRHSRIEDGIEVSDFSNGTDDDEWTWESIRDLHTISYGISRIQLLPIPVKK
                     AMVRNVLAVPLNAEVSECALKMRRARIDQLPVINGNRRLVGMLFDRELIKVLLPERQD
                     LRKS"
     gene            3724..4368
                     /gene="psmB"
                     /locus_tag="PK196_05740"
     CDS             3724..4368
                     /gene="psmB"
                     /locus_tag="PK196_05740"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HON40954.1"
                     /translation="MLDISHEIMKGTTTVGLIFDRGVVLATEMRATMGNMIASKRAKK
                     IYQITPRIGLTTAGGVGDAQQLVRIMQVECNLFEMRRGKTMSVGAASTLLSNYLNQNR
                     YYPYYVQLLMGGFDDEGPSIYSVDALGGATREDDIVATGSGSPFAYGVLEDQYRPDMK
                     EDAAKDLAVRAVRSAMRRDSASGEDIMVVVITKNKYDERILGGLYEPSEARPAA"
     gene            4390..>5989
                     /locus_tag="PK196_05745"
     CDS             4390..>5989
                     /locus_tag="PK196_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HON40955.1"
                     /translation="MVIEDKLQELKEQINRIVPGGITVSDVEFEGPELVIYTDDPKQF
                     ADQADLIKILARDLRKRIVVRPNILEDPERAAQEIRAVVPENAGITEIFFDPETGEVL
                     IEAEKPGVVIGKNGATLREITKQIGWTPKVVRTPPIESSTVKQIRAYLRSVKDERKVF
                     LRKIGRRIHREVTSKDQWLRVTTLGCCREVGRAAFLISTPESKVLVDCGEKPGSTTNG
                     TPYLYVPEIYPLDALDAVVVTHAHLDHCALVPLLYKYGYDGPVYSTPPTRDLAAMLQL
                     DYIDVVRKETDRIPYSSNEVKAYIKHSITLNYGSVTDIAPDIKLTFHNAGHILGSAIA
                     HFHVGDGLYNIAFTGDFNYQKTRLFSPAVPSFPRLEALFMESTYGGSNDIQPQRKEAE
                     EKLYEKVSETIGRGGKVIIPAFAVGRSQEVMLALEEGIRRQKLPPVKIYLDGMIKEAT
                     AIHTTYPEYLNSDLRNQIFREGLNPFLSEAFVQVDSPDLRERVITGEPCVIITTSGML
                     NGGPVMEYLKALGPDERNSLIFVGY"
     assembly_gap    5990..6089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6090..6437
                     /locus_tag="PK196_05750"
     CDS             <6090..6437
                     /locus_tag="PK196_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HON40956.1"
                     /translation="YLKALGPDERNSLIFVGYQAEGTLGRRIQKGWREIPLGWRDTIV
                     INLEIVTVDGFSGHSDRKQLMNYVAHLQPRPEKIFTIHGDENKTLDLASSIYKRYRIE
                     THSPLNLETYRMI"
     gene            6439..7506
                     /locus_tag="PK196_05755"
     CDS             6439..7506
                     /locus_tag="PK196_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON40957.1"
                     /translation="MLQRFTLLLGVFVVMALSNAVVPVLPDFAEGTAFQGAVYSAYFL
                     GAFVSVLPAGYASDRIGSVPLIRAGLLLTLLSGTLILVFPSGLPLILSRVIEGIGGGI
                     FLSATLAWTNSQPSAARESGYVMAALNLGLFVGLIAAGWLDSVFESHLAGIAVFTALA
                     VPAAAMSAAVRESERAGFVRADVAGIVRNYFWLFAAVMVLVGVTGAVAAIYPGYTGND
                     STVIALQLATINIATVGAVLVAPRFNLRPVPAIRASALLMAPAVAVGCVTPYAFPVIG
                     ALSGVTIVAVLEFLAATGIQQGVMTGLFNTASYAGFTILSALAGGVAGWFGFLPAFLL
                     LAVMVAAAAVPIGRCRCEYSG"
     gene            7547..>8886
                     /locus_tag="PK196_05760"
     CDS             7547..>8886
                     /locus_tag="PK196_05760"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HON40958.1"
                     /translation="MKMLIDGEWCGSVSGKVFPVHNPATGEVVDQAPLGVEDDLRAAV
                     EAAGEAFSDWAAKEPRVRAKILFSAAEEVRRRNAELGALLTAEQGKPIREAIDEINGF
                     ANILEYYYALSAGLQGEFMDIRGYGRATVIRRPLGTCGAIIPWNMPAIIMGWKIGAAL
                     TAGNTMVVKPARTAPLTCMRLAEILEEAGLPPGVLNVVTGSGETVGREIARNPAIRKV
                     SFTGAVETGREVAMDAAPAMKHLTLELGGSDPMIVCGDADIPAAIEGVIRGRFYNCGQ
                     VCTAVKRLYIFDSIADEFIRRLKTRVERIVVGNGIDRGVDMGPMNNREGLERIAGLVD
                     AVRERGEGEIVTGGQAPRGEALRDGNFYLPTLLTGLPDDSPLFTEEVFGPVLPIAAVR
                     DLDEAIERANSSRYGLGASVWTRNPDVIARAAEDVEAGIVWVNQHLRIPPEVPFG"
BASE COUNT         1810 a         2509 c         2703 g         1764 t
ORIGIN      
        1 tctgctgtca acgtgaagac cctaccgaaa tcctgttcct gctccccgtg cacggcatcc
       61 atcttgcggc aaccgttacg tactgccgcg ttcggggatg aactcacctg agggaaggag
      121 agcgtgcccg cccccccggg atcgggcgtg cagcagaccc cggaaagatg gcatcaagct
      181 gcatggaacc cgagcctttt tctggaagcg gagacgacca ctgatacgtg aattcgatgg
      241 agatccaggt attcggtgtt cggggtcttc ccctgatccg gcagggcgac gacctgccag
      301 gcatgatctg tgagcgggtc gcgtttgagg atggcgatat cctgacggtt gcctcatcgg
      361 tctactccaa ggcaaaaggc ttcacccgtg accttgaatc cattgtcccc ggtgctgatg
      421 ccgtgcggat agccggaaag acaggagagg acccgcgctt tgtgcaggcg gtgctggact
      481 cctctgctgc gatactgctc gaagacccgt tcatcctctc cgaactgcct ttcggccaca
      541 tcggtgtgcg ggccggcgtc gaccacagca acatcgagga tggccgggtg atcatcctcc
      601 cccccgatcc gatggccgcc gccgccgaga tgagggatgc gatccatcgg atcagcggaa
      661 aagacgtccg cgtgataatc accgatacct gcggccgggc gttccgccgc ggccagaccg
      721 gcatcgccat cggctgggcc gggatgacag ccattgacga ctaccggggc gacaccgacc
      781 tctttgggca tgtcctcaag atcacagaag aagcggtggt cgacgagatt gctgccttcg
      841 caaactttgt catgggcgag agccaccagg gtgtacccgc ggtggtcttc agaaactgcc
      901 gcacctggaa agggcacgat gctgtatact tcaccccgga ggaagacatc atcagggctg
      961 cgctgctaaa ggagcataaa aaagattaga agaggagatt tagtatataa actgccactc
     1021 cggtcgcgag ggctatcaca agaacggctg tcgggctgat atggatggcc cggtggtcgt
     1081 cgctgtcgta gtaattgacc aggccggcag aggataccag tctcccgcca gatttttttg
     1141 ccataccaga taattatcat ttgcaatata taaaaccgag gttgaaccaa tgcggcagcc
     1201 tgaagattgc gtcataaccg gccgggcgct cctcggcgaa gaactcctgg aagaggacgt
     1261 cagtataacc gtctcgggcg gcatcgtcac ctcgatagag cccgtctccc ggacgcccgg
     1321 gctctggatc gtgccggcct tcttcaacgc ccacacccat ctcggggaca ccgtcgcgat
     1381 ggacatcccc gcccgtggaa gcctggccgg cctggtcaaa ccgccgaacg gtttgaagca
     1441 ccggatcctt gcagcaaccc cgcgcgccga cctggtcagg gggatgcgcg cgagcatcat
     1501 caccatgatg gcaaccggga cggccgggtt tgccgatttc agggaaggcg gggctgatgg
     1561 cgttgcagcg ctgcatgagg cggcagcggg gcttgactgt tccccggtca tcctgggccg
     1621 cgatggagga gagacggtcg gtgacggggc ggggatcagc agtgttcatg acgtgaaaaa
     1681 cgctgaagag atcgtgagat gtgcgcgtgc cgcagggaaa cttgtcgcat tccatgccgg
     1741 ggagaagagc ccggacgaca tcgatgaggc gctcgcgttt gaacccgacc tgctcgttca
     1801 ctgcacccat gcaacagatg cgcaactgcg ccggatcgtc gatatgggaa taccggtcgc
     1861 cgtctgcccc aggtcgaact gggtgctcgg ggtcgcggca tcacccgccc acccaccgat
     1921 cgcgcgcatc atcgaactcg gcggcaccct ttacctcggg acggacaacg tgatgtttgt
     1981 tcagccggat atgctccagg agatggcgtt tactgcaacc gtctatcgcg catccccgga
     2041 agagatcctg cgtgccgcga tcgcgggcgc gaggcttgcc ggggtctccg gataccttga
     2101 agcggggcag aaggcgcgtt tcatcgttgt aaatccgtta aaaagcaatc tgtctttcag
     2161 cagggacatc cgtgcgacca ttgtaaaacg gctggattcg tcatcgatcg gccaaaatat
     2221 tttaaccctg caatagaaat gagatacgca gtatctgttc tgcgtggtgt gtatgttccg
     2281 taagatactt gttgctatag atggctcaga acaggctgac cgggcttttg aggcggctct
     2341 cgcggaggcc ggcgtctgga aagctgaggc tcatgttgtg tatgtggttg aatcggggct
     2401 cttttcatct cttcccatgg acagcacact cgagataata tacagtgttc tgcataaaga
     2461 aggtgaggag atactcgaat cggccagaaa gaaagcagag acagcaggta ttccgttgac
     2521 cacccacctt tgccaggggc atgtgggttc tcagatagtc tccatggcaa aggaactggg
     2581 tgctgacctc atcgtccttg gttcatacgg gaaaagcggg gttgatcgtc tcctgctcgg
     2641 gagcgcgacc gactacgtgg tgcgaaacag tcccgttacg acgatggtgg tgagatcata
     2701 gcctcgcccg agcaaattct tgtccgggac ttcatggtga ccgatgttgt ctgcgtcgag
     2761 atcccgggca accgcgatga tgtcctgcgt atcttaaaac gcaccggcat cagcggtgtc
     2821 cctgtcttaa aggaaggtga acttgtcggc attatcaccc gcaaagacct cctccgtaag
     2881 gctgaagaga cacagttagg gctcctgatg acacccgacc cggttgttat caggccggac
     2941 gcccccatct cagaggcggc gcaactgctt gtgcgccaca acatcaggag gctcccggtc
     3001 atcgaggatg gggcgcttgt cgggattctc agcgtcgccg acctgatcaa cgccatagca
     3061 cagatgcgta tcaccatacc catcaaggac aacttcgtaa gcaagacgta tgcgctctgg
     3121 gaagagaccc ccctcacgct tgtcgggagg atcatggaga tctcaggcta cgacgccata
     3181 cccatcctta tggaggatgg cacgctcagc ggaatcatat cggagcgcga cctcatccgc
     3241 cattctcgca tcgaggatgg tatagaggtc agtgacttct caaacggcac cgacgatgac
     3301 gaatggacat gggagagtat acgcgatctg cacaccatca gttacggtat ctcaaggatt
     3361 cagctgctcc cgattcctgt taaaaaggcg atggtcagga acgttcttgc cgttcccctc
     3421 aacgccgagg tcagcgaatg cgccctcaag atgagacggg caaggatcga ccagctcccg
     3481 gtgatcaacg ggaacaggcg gcttgtcggg atgctgtttg accgtgagtt gatcaaggtc
     3541 ctgctgcccg agcggcagga cctgcgaaag agttaaagta taacgtccat ataccttatg
     3601 gatgcctgta tctgttgcat ctgttttatg atgcggcgat aagtttatga tttaacgtat
     3661 caccatacgc ttaaccgctt ccaggcgaga taaagcgttt taatggtttt aaggggtgtt
     3721 tcaatgcttg atatttccca cgagattatg aaaggtacaa caactgtagg actgatcttc
     3781 gaccgtggcg tcgtgcttgc aaccgagatg cgggcgacga tgggcaacat gatagcaagc
     3841 aagcgggcca agaagatcta ccagatcacg ccaagaatcg gtctgaccac cgctggaggc
     3901 gtcggcgatg cacagcagct cgtccggata atgcaggttg agtgcaacct ctttgagatg
     3961 cgccgcggaa agaccatgtc tgtaggtgca gcctcgacgc tgctatcaaa ctacctgaac
     4021 cagaaccgct actacccata ttacgtccag ctcctgatgg gcgggttcga tgatgagggg
     4081 ccgagcatct actcggtcga tgcccttggc ggggcgacca gagaggacga tatcgttgcc
     4141 acaggttcgg ggtctccatt cgcctacggt gtgctcgagg accagtatcg tccggacatg
     4201 aaggaggatg cggcaaaaga cctggcagtt cgtgccgttc gttctgctat gcgccgggac
     4261 tcggcatctg gtgaggatat tatggtggtc gtgatcacga agaacaaata cgatgaacgc
     4321 atcctgggcg ggctgtatga accctctgaa gcccgccctg cagcctgaca ttttttcagg
     4381 acggtcttaa tggttattga agataaactc caggagctca aggagcagat taacaggatt
     4441 gttcctggtg gaattacggt ctctgacgtc gagttcgagg gtcccgaact cgtcatatat
     4501 accgatgatc cgaaacagtt cgccgaccag gcggacctga taaagatact ggcacgggat
     4561 ctcaggaaac gtatagtcgt gcgcccgaat atccttgaag acccagagcg ggcggcgcag
     4621 gagatccggg cggttgtgcc ggagaacgca gggatcaccg agatcttctt tgacccggag
     4681 accggcgagg tgctgatcga ggctgagaaa ccgggtgttg tcatcgggaa gaacggggca
     4741 accctccggg agatcacaaa acagatcggc tggacgccca aggtcgtccg gacaccgccc
     4801 atcgagagtt cgacggtcaa gcagatccgt gcctatctcc gctcggttaa ggatgagcgg
     4861 aaagtgttcc tccgaaagat cgggcgccgc atccaccgtg aggtcacgag caaggaccag
     4921 tggctgcggg tcaccaccct cggctgctgc cgtgaggtcg gccgtgccgc gttcctgatc
     4981 tcgacgccgg agagcaaggt cctggttgac tgcggggaga agcccggcag caccacaaac
     5041 gggacgccct acctctacgt gcccgagatc tacccccttg acgcgcttga tgctgtggtg
     5101 gtcacacacg cgcaccttga ccactgtgcg ctggtgcccc tcctctacaa gtacggctac
     5161 gatgggccgg tctactcgac cccgccgaca agggacctgg cggcgatgct ccagctcgat
     5221 tacattgacg tcgtcaggaa ggagacggac aggatccctt actcctcaaa cgaggtgaag
     5281 gcctacatca agcactccat aaccctcaac tacggcagcg tgaccgatat cgctccagac
     5341 atcaagctca ccttccacaa cgccgggcat atcctgggtt cggcgatcgc gcacttccat
     5401 gttggtgacg gactctacaa catagcgttc accggggatt tcaactacca gaagaccagg
     5461 ctcttctctc ccgcggtccc gtcattcccg cgtcttgagg cactattcat ggagagcacc
     5521 tacggcgggt caaacgatat ccagccccag aggaaggagg cagaagagaa actctatgag
     5581 aaggtctccg agaccatagg ccgcggcggc aaggtgatca tcccggcgtt cgccgtcgga
     5641 cggtcgcagg aggtcatgct cgccctggag gaggggatcc ggcggcagaa actcccgccg
     5701 gtgaagatct atcttgacgg tatgatcaag gaggcgacgg cgatccacac gacatacccg
     5761 gagtacctca acagcgatct ccggaaccag atcttccggg aggggctgaa ccccttcctc
     5821 tcggaggcct ttgtccaggt ggactccccc gacctccgcg aacgggtgat caccggtgaa
     5881 ccctgcgtca tcatcaccac aagcggcatg ctgaacggcg ggccggtgat ggagtacctc
     5941 aaggcgcttg gccctgatga gcgcaactcg cttatcttcg tcggttaccn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acctcaaggc gcttggccct gatgagcgca
     6121 actcgcttat cttcgtcggt taccaggcgg aagggacgct cgggcggcgg atccagaagg
     6181 gatggcgcga gatcccgctt ggctggcgcg atacgatagt gatcaacctt gagatagtca
     6241 cggtcgacgg gttctccggc cactccgacc gcaagcagct gatgaactat gtcgcccacc
     6301 tccagccgcg gccggagaag atcttcacca tacacggtga cgagaacaag accctcgatc
     6361 ttgcaagttc catctacaag cgctaccgca tcgagactca ttctccattg aacctcgaga
     6421 cataccgcat gatctaggat gctgcagaga ttcaccctcc tgctcggtgt atttgtggtg
     6481 atggcgctct caaacgctgt cgttcccgtc ctccccgatt tcgccgaagg gacagcgttt
     6541 cagggtgcgg tctactctgc atacttcctt ggggcgtttg tctccgtgct gccggcaggt
     6601 tacgcaagcg accggatagg gagcgtgccg ctcatccgtg ctggacttct cctgacgctt
     6661 ctaagcggca ccctcatcct ggtttttccc tccggcctgc cgctcatcct ctcccgggtt
     6721 atcgagggga tcggcggcgg aatcttcctc tcggcaacgc ttgcatggac aaattcgcag
     6781 ccctcggctg cccgtgagag cggttacgtc atggcggccc tgaacctggg gctgttcgtc
     6841 gggcttattg cagcgggctg gctcgattcg gtgtttgaga gccatcttgc cgggattgcg
     6901 gtctttacag cgcttgctgt cccagcagcc gcgatgagcg ccgcggtcag ggagagcgaa
     6961 cgcgctggtt tcgtgagggc agacgttgca gggatcgtgc ggaactactt ctggctcttt
     7021 gctgcggtga tggtcctggt gggggtgacg ggggcggtgg ctgccatata tcccggctac
     7081 acggggaacg actcaacggt gattgccctc cagcttgcaa ccatcaacat cgcaaccgtc
     7141 ggtgccgttc tcgtcgcacc gcgcttcaac ttacggccgg ttccggcaat ccgggcgtcc
     7201 gcgctgttga tggcgccggc ggtcgcagtg ggctgcgtca cgccatatgc cttcccggtc
     7261 atcggcgctc tctcgggggt gaccatagtc gcggttcttg agttcctcgc agcgaccggg
     7321 atccagcagg gggtgatgac cggactcttc aacaccgcct cctacgcagg gtttaccatc
     7381 ctttccgccc ttgccggcgg agttgccgga tggttcgggt ttttgccggc gttcctccta
     7441 ctggcagtga tggtggcggc agcggccgtc cctatcggca ggtgccggtg cgaatacagt
     7501 ggttgacccg gagcgcatat taccgctcct gccgatagga tcacgtatga agatgttgat
     7561 cgacggcgag tggtgcggct cggtctccgg gaaggtcttt ccggtgcaca accctgccac
     7621 gggagaggtt gtcgaccagg caccgctcgg cgtggaggat gatctccggg ccgcggtgga
     7681 ggcggccggg gaggcgtttt cagactgggc cgcaaaagag cccagggttc gggcaaagat
     7741 tctcttctct gcggcggagg aggttcgccg aaggaatgcc gagctcgggg cactcctgac
     7801 cgccgaacag ggaaaaccca tccgcgaggc gatcgatgag atcaacgggt ttgcaaacat
     7861 cctggagtat tactatgctc tttcagcggg gctgcaggga gagttcatgg acatcagggg
     7921 atacggccgc gccactgtca tcagaaggcc gctcggcacc tgcggcgcga tcatcccctg
     7981 gaacatgccg gcgatcatca tgggctggaa gatcggcgcc gcgctgacgg caggaaacac
     8041 catggtcgta aaaccagccc gcacggctcc gctgacctgc atgcgactcg ctgagatcct
     8101 ggaggaggcc ggtctgccgc cgggggtttt aaacgtcgtc acgggttcgg gggagacggt
     8161 cggccgggag atcgcgagaa acccggcaat ccggaaggtc tcgttcaccg gcgcggtcga
     8221 gacagggcgt gaggtcgcga tggatgccgc gccggcgatg aaacacctca ccctggaact
     8281 cggggggagc gacccgatga tcgtctgcgg cgatgccgat attcctgcgg ccatcgaggg
     8341 tgtcatcagg ggacggttct acaactgcgg ccaggtctgc acggcggtaa agaggctata
     8401 catctttgac tcgatcgcgg atgagttcat ccgccgattg aagacgaggg tcgagaggat
     8461 cgttgtcggg aacgggatcg accgcggtgt cgatatgggc ccgatgaaca accgcgaggg
     8521 tcttgagagg atcgccggtc tggtggatgc cgtgcgggaa cgcggtgagg gcgagatcgt
     8581 caccggtggt caggcgcccc ggggagaggc gttgagagat ggcaacttct acctgccgac
     8641 acttctcacc ggtcttccag acgactcacc cctcttcacg gaggaggtct ttggcccggt
     8701 gctgccgatc gctgctgtcc gtgacctgga cgaggcgatt gagcgcgcaa acagcagtcg
     8761 ttacggcctc ggtgcatcgg tgtggacgcg gaacccggat gtgatcgcca gggcggcgga
     8821 ggacgtcgag gccggaatag tctgggtcaa ccagcatctc aggattccac cggaggtgcc
     8881 gtttgg
//
LOCUS       DAOSZX010000058         8844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6738_length_8844_cov_20.386733, whole genome shotgun sequence.
ACCESSION   DAOSZX010000058 DAOSZX010000000
VERSION     DAOSZX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_8844_cov_20.386733"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..682)
                     /locus_tag="PK196_05765"
     CDS             complement(68..682)
                     /locus_tag="PK196_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HON40959.1"
                     /translation="MSALEDLITKAKALQAEGHTPDQISDELGLSMETVTWLLTQQKG
                     IDSPKDVHIDWTAVGSHGALLSDMAMVMLKRFLYLTDDGSLDRDEERAGTVVGIAQSG
                     VPLATLIAAEEGLRLAVYLPAKYSRGETPTGSLWGTFSTIAGQRCIIVDDVITTGMTL
                     SETIAFLRRHGATPVAVWSLFDKRGIREVEGVPVHSLFVISRLG"
     gene            complement(704..1159)
                     /locus_tag="PK196_05770"
     CDS             complement(704..1159)
                     /locus_tag="PK196_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HON40960.1"
                     /translation="MTLVLDASAFFVDVPVDGPAWTTPSVVDELVDFHAKCRFTALSA
                     AGLSVREPRREDLLQVDEAARQTGDAGVLSVTDREILALALELGAVLVTDDFAVQNVA
                     HHLGIETRSILQRPARPVQWRYRCTGCGRYWREPGDCPVCGAAIKRKLK"
     gene            complement(1156..2139)
                     /gene="rtcA"
                     /locus_tag="PK196_05775"
     CDS             complement(1156..2139)
                     /gene="rtcA"
                     /locus_tag="PK196_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HON40961.1"
                     /translation="MLTIDGSHLEGGGGIIRMAVALSALSATPIRVIGIRQNRPNPGL
                     APQHVAAVLAAAGTCDAECTGLSQGSGEITFTPGRVRRTDLRIETGTAGSIPLVIQAW
                     LPAALATGGSITVSGGTEVAWSPTIDYLDRVFAPVLRRAGVSLEVEVMRRGYYPVGGG
                     MARVRVEASCPGLIAILAEEDSCGLVSCSANLPAHVAERQAAAARELLELRAGLSCTA
                     EIDSRGHGLSVGSSITVWKGAKGAVALGRRGLPAEEVGRAAARALLEECRAPGVVDVH
                     LADQLLIYLACYGGEYTTHTLSMHAKTACWLLESFGYPVRCRENDTVEFSA"
     gene            complement(2126..2983)
                     /locus_tag="PK196_05780"
     CDS             complement(2126..2983)
                     /locus_tag="PK196_05780"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HON40962.1"
                     /translation="MRIISTERSQVLAARVAEKLGVPLVETIFRRFPDGELYLKCGEL
                     DDETLIISSIVDNDMFVQTLLAIDAADRSMNTLVVPYLGYSRQDKRFSPGEPISARTI
                     ARALSTGVGRVYVINIHDRSVLRYFGVPSKEITIAPAVGSYVGDLRLKNPLVLAPDEG
                     AIGLASEVAAVGGWDCDHLVKTRLSGEEVRIAPKTVDVAGRDVVIVDDIISTGGTLAT
                     AARMLRDQGANSIHAACVHGVLTSGAYTRLRAAGVSSVVSSDTCESASSLISAANAIA
                     TAIGEDAHH"
     gene            3119..5005
                     /gene="lonB"
                     /locus_tag="PK196_05785"
     CDS             3119..5005
                     /gene="lonB"
                     /locus_tag="PK196_05785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HON40963.1"
                     /translation="MEETDLLAGLEVSTSSDIDVPPKLIDQVIGQEHAVEVIKKAAVQ
                     RRHVMMIGTPGTGKSMLAKAMAELLPKEELQDILVYPNPEDGNNPIIRTVPAGRGKQI
                     VNAHKAEARKKIQMRSTLIMLLIIGIIVYAIITYQWLMGIIAAAFIFMALRYSMPRED
                     AMVPKLLVSRDSNNTAPFVDATGSHAGALLGDVRHDPFQSGGLETPAHDRVEAGAIHR
                     AHGGVLFIDEINTLTPHSQQNLLTALQEGSFPITGQSERSSGAMVRTEPVPCQFVMVA
                     AGNVDAIQGMHPALRSRIRGYGYEVYMRETMEDTPENRKKFLRFIAQEVKNDGKIPHF
                     DRSAMLEVLREARRRSNRKGHLTLKLRDMGGLIRVAGDLARQEGAELTTARHVLAAKS
                     ASRSIEDQISDEYIRRTRDYDITIVEGTRVGRVNGLAVMGSDSGTVLPVMAEVTPSQG
                     ANGTVIATGMLKEIAQESIKNVSALIKKFTGRDIRNMDIHIQFIGTYGGVEGDSASVT
                     VATAVISAIENIPVRQDVAMTGSLSVRGDVLPIGGVTYKIEAAAKAGIKKVIIPRSNL
                     DDVLIEDRYRSMIEIVPVDHIEEVLQNALVPENREGFLSKLRKLAANSAGGILDPNLG
                     HPAA"
     gene            5011..6339
                     /locus_tag="PK196_05790"
     CDS             5011..6339
                     /locus_tag="PK196_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON40964.1"
                     /translation="MSDVRYYDIRRVRGEVTRIDIDNGVVESAATSFFDRTVIRALAS
                     NGWGILTRDHVDLDSKHEIETLVREAARLAAVTKDRLDLADAPRGALPIPPLGEDPRE
                     IDIEEKTRLLAEIEGAARLPGVTNTRANYTEGIDSVRFLDSEGNEYSYQSVRSGFSVV
                     AIASRNGVMQMGSEREHTIRGFNLRGRHDLGRRAGEVAVSLLDAKIPKGGMQRAVLDP
                     ELAGVFTHEAVGHASEGDLVREGASVLGGRIGEHIGCDGLVIVDDPALHEFGFMPVDA
                     EGVAVQRTEIIRDGILASYLHNRETLAAVGDGMPGHARAEHGAPPIVRMSNTFIENGD
                     ATREEILAECREGILLIGSRGGQVDPGRGVFQFNAEYGYLIEGGETTGMLRDVSLSGE
                     ILRTLHNITLIGDDRRMNPGYCGKGGQVVPVSDGAPHLLLEDATIGGRGE"
     gene            6336..7628
                     /locus_tag="PK196_05795"
     CDS             6336..7628
                     /locus_tag="PK196_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HON40965.1"
                     /translation="MNGEDLIEMVLREGERLADEVEVFSARGQSVSTEIKRGIIGVAE
                     ESEAWSMAIRTVRNGRIGFSSTGDPDRWRDCLDAALASGRLATPQRWEGFPGPAAFSG
                     VPSSADRGLEVAIEDAARMVDDLLAGAAEHPVDVIGGSADLGRSHLSIANSNGVSYEM
                     ERTVVGVSLETIREQSTGYEFDASPFRRDIDARAVGERAAMLAARSLGGRDIETGRYD
                     IVLSPVAAASLLGQVLVPALSGRNVKAGRSPLADRVGEQVFDGSLSVYDDPFAPGPGS
                     TAWDAEGVPTRRLLFIEQGVVRGFAYDLKTGYRYGEESTGSAVRGGNEPPAIGVHNLF
                     INGAREKEPGYGRAVWIHDVVGAHTANPFSGDFSVEVSNPFWMEDGELIEPIRAAMLS
                     GNVFEMLGEIVGFGNDARRVGRLTIPSIRLNNQQIIGK"
     gene            7629..7892
                     /locus_tag="PK196_05800"
     CDS             7629..7892
                     /locus_tag="PK196_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HON40966.1"
                     /translation="MMEEIFAILLAVAIAAALYYLMKKALTLVINAIAGLITLWALNY
                     FSVLAWFGAPDIQISLVTILICALGGLPGALILVLLHLIGITI"
     gene            complement(7932..8597)
                     /locus_tag="PK196_05805"
     CDS             complement(7932..8597)
                     /locus_tag="PK196_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HON40967.1"
                     /translation="MGLDTMLSGGLIENSICALVGTYGTGKTMLALQFAYEGLSRGEK
                     VIYISLEEREELLRATMAQKGWDPGAFGDRFYLLKLDPTDFNLAINSIKSELPALIES
                     TGASRVVIDPISLFEGLFEDESERRREMFRFVEVMRDEACTLVLTSETCPGNPEESRY
                     GIIEYLADTVILLRYIRSPGLTEVHLALEVVKMRRSAHSREIKPFEILQDGILVHSEA
                     SLF"
     gene            complement(8627..>8844)
                     /locus_tag="PK196_05810"
     CDS             complement(8627..>8844)
                     /locus_tag="PK196_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40968.1"
                     /translation="DRSRELEIADCVDATFIFQWEESGAQRRQRVMYFGKFRGVMTRL
                     EDKNLVKFAVSISAKGGFEVRNIRMVV"
BASE COUNT         1830 a         2763 c         2663 g         1588 t
ORIGIN      
        1 gcaacataga tcataactgg ctggataccg gaggctgaag taaattcaat cgacttttca
       61 ggccatctca accgagcctg gatatcacaa acagcgagtg taccggaacg ccttctacct
      121 ccctgatgcc ccgtttgtca aagagcgacc agaccgccac cggcgtggcc ccatgccgcc
      181 ggaggaaggc gatcgtctcg gagagtgtca tcccagtcgt gataacgtcg tcgacaatga
      241 tacagcgctg cccggcaata gtggagaatg tcccccagag ggacccggta ggcgtctcac
      301 cccgactgta tttcgccggg aggtatactg caagcctcag cccctcctcc gcggcgataa
      361 gggttgcgag cgggacgcct gactgcgcga tacctacgac cgtcccggcc ctctcctcat
      421 cgcggtccag gcttccatcg tccgtcaggt agaggaagcg tttgagcatc accatcgcca
      481 tatcagatag gagcgcaccg tgacttccta cagccgtcca gtcgatgtgg acatcctttg
      541 gagagtcaat acccttttgc tgggtgagca gccaggtgac cgtctccata gagagaccga
      601 gctcgtcgga gatctggtcg ggggtgtgtc cttccgcctg tagcgctttt gccttcgtga
      661 tcaggtcttc gagcgcggac atgtagaaga gatctccgag cgattattta agttttcttt
      721 tgattgccgc accacagacc ggacagtctc ccggttcccg ccagtaacgc ccgcacccgg
      781 tgcagcggta gcgccactgg accggccggg ccgggcgctg caggatgctc cgcgtctcga
      841 tgccaaggtg atgggcgacg ttctggacgg caaaatcgtc tgtgacaagg actgcgccga
      901 gttccagtgc aagcgccagg atctcgcggt cggtcacaga gagcacccct gcatcccccg
      961 tctgacgcgc agcctcatcc acctgtagca ggtcttccct gcgcggctcc ctgacactga
     1021 gccccgcggc agagagagcc gtgaagcggc actttgcatg aaaatcgaca agttcatcca
     1081 ctaccgaggg tgttgtccag gcggggccat cgaccggcac atccacaaag aaggccgatg
     1141 cgtcaagtac cagcgtcatg ccgagaactc cactgtatca ttctcgcggc acctgaccgg
     1201 gtaaccaaat gattcaagca gccagcaggc ggtcttcgca tgcatcgata gagtatgcgt
     1261 cgtatactcc ccaccgtagc aggcaaggta gatcaggagc tggtcggcca ggtggacgtc
     1321 aacaacaccc ggcgcgcggc actcctccag gagggcgcgg gcagccgccc gaccaacctc
     1381 ctcagccggg agaccccgtc tcccgagagc gacggcgcct ttagcacctt tccatacggt
     1441 gattgaactc ccgacgctca acccgtgccc gcgggaatcg atctccgccg tgcaggatag
     1501 acccgcccgc aactccagga gttccctggc ggctgcagcc tggcgctccg cgacgtgtgc
     1561 aggaagattg gctgaacagg aaaccagccc gcaggagtct tcctctgcca ggatcgcgat
     1621 cagaccaggg caggatgcct ccacacggac acgcgccatt ccaccaccca ccggatagta
     1681 gccccgcctc atcacctcaa cctccagaga gactcctgca cggcggagca ccggcgcaaa
     1741 tacacgatca agataatcga tggtcgggct ccacgccacc tctgtccccc cggagaccgt
     1801 gatgctcccg ccggtggcaa gggcagcggg gagccaggcc tggatgacca gcgggatgct
     1861 ccctgcggtc ccggtctcga tccgcagatc tgtcctcctg acccgccctg gagtaaaggt
     1921 gatctcgcca ctcccctgag aaagacccgt gcactctgca tcgcaggttc cggcggccgc
     1981 cagcaccgca gcgacatgtt gcggggcaag cccggggttc ggccgattct ggcgtatccc
     2041 gatcactctg atcggcgtgg cggagagggc cgagagggcc acggccatgc ggatgatccc
     2101 tccccctcct tcaaggtgag agccgtcaat ggtgagcatc ttccccgatc gcggtggcga
     2161 tggcattggc ggccgatatc aggcttgaag cgctctcgca ggtatcgctg gagaccaccg
     2221 atgagacccc cgccgcccgc agacgggtgt aggcaccgct tgtgagcacc ccgtggacgc
     2281 aggcggcgtg gatggagttg gccccctgat ccctgagcat ccgggcagcg gttgccagag
     2341 tcccgccggt cgatatgatg tcgtccacta tcacgacgtc ccgaccggcc acatcgacgg
     2401 tctttggagc aatcctgacc tcttcaccgg aaagccgggt ttttacaagg tgatcgcagt
     2461 cccatccccc caccgctgca acctcagagg cgagaccgat cgcaccctca tccggggcga
     2521 gaaccagggg gtttttcagg cgcagatcgc caacgtaact cccgactgcg ggggcgattg
     2581 tgatctcctt cgacgggacg ccgaagtacc tcagcacgct tcgatcgtgg atgttgatga
     2641 catagacacg cccgacacct gtcgagaggg ctcgcgcgat cgtcctggcg ctgatcggct
     2701 ctcccggaga gaaacgctta tcctgccggg aatacccaag gtaaggcacc accagcgtgt
     2761 tcatcgacct gtcggcagca tcgattgcaa gaagggtctg cacaaacata tcgttgtcga
     2821 cgatgctgct gatgatcagg gtctcatcgt ccagttctcc gcatttcaga tacagttccc
     2881 cgtcgggaaa ccggcgaaat attgtctcaa ccagcggtac ccccagtttt tcggcaacgc
     2941 gggctgcgag aacctgggac cgctctgtgc tgatgattct catgggtcac ctttgctaga
     3001 aagtatttga acgaggatga ataaattata taactaccac tgcatcaaag aggtaaactc
     3061 ggcaatggat tcaacaagac caacagaaat ttcagacata gacctcaccg acgaggagat
     3121 ggaagagacg gatctcctcg ccggtcttga ggtgagcaca tcttcagata tcgatgtacc
     3181 cccgaaactg atagatcagg tcatcggcca ggagcatgcc gtcgaggtga tcaagaaggc
     3241 tgcggtccag cgccggcacg tgatgatgat cggcacccct ggaaccggca aatcgatgct
     3301 ggcgaaggcg atggccgaac tcctcccaaa agaggaactc caggacatac tggtctaccc
     3361 gaacccggaa gatggcaaca acccgatcat acgaaccgtg cccgctggcc gcggcaagca
     3421 gatagtcaac gcccacaaag ccgaggccag gaaaaagatc cagatgcgga gcactctcat
     3481 catgctcctg atcatcggga tcatcgtcta cgcgataatc acctaccagt ggcttatggg
     3541 cataatagcc gccgccttca tcttcatggc gttgagatac tcgatgcccc gcgaagatgc
     3601 aatggtccca aaactcctgg tatcgcgcga ctcgaataac acggcaccct tcgtcgatgc
     3661 caccgggtca cacgcagggg cgctgctcgg cgacgtccgg cacgacccct tccagagcgg
     3721 ggggctcgag acaccggcac acgaccgtgt agaggccggg gcaatccacc gggcgcacgg
     3781 cggcgtgctc ttcatagacg agatcaatac cctcacaccc cactcacagc agaacctgct
     3841 gacggcgctc caggagggat ccttcccgat caccggccag agcgaacgct caagcggtgc
     3901 gatggtcagg accgagccgg tcccatgcca gttcgtgatg gttgctgcag gaaacgtcga
     3961 tgccatccag gggatgcacc ccgcactccg gtcgcgtatc cggggatacg gctatgaggt
     4021 atatatgcgc gagacgatgg aagacacccc cgaaaaccgg aagaagttcc tccggttcat
     4081 agcccaggag gtaaagaacg acggcaagat ccctcacttt gaccggagcg ccatgcttga
     4141 ggttctccgc gaggctcgcc ggcgctcgaa ccggaagggg cacctgaccc tgaagctccg
     4201 tgacatgggc ggtctcatcc gcgtggcagg tgacctcgcc cggcaggagg gcgcggagtt
     4261 aaccacggcc agacacgttc tcgcggcgaa atccgcctcg cgttcgatcg aggaccagat
     4321 ctccgacgaa tacatccgga gaacccgcga ctacgatatc acgattgttg agggcacacg
     4381 ggttggccgg gtgaacgggc ttgcggtgat ggggagcgac tccggcaccg tgctccctgt
     4441 gatggccgag gtgacgccga gccagggggc aaacggaacg gtcatcgcca ccgggatgct
     4501 caaggagatt gcccaggagt cgatcaagaa cgtcagcgcc ctgatcaaga agttcactgg
     4561 cagggatatc cgcaacatgg atatccacat ccagttcatc ggcacctacg ggggtgttga
     4621 gggggattca gcctccgtga ccgtggcaac ggcggtgatc agcgcgatag agaacatccc
     4681 ggtgcgccag gacgtcgcga tgacaggttc actatcggtc aggggcgacg tcctccccat
     4741 agggggggtc acctacaaga tcgaggcggc cgcaaaagcc gggatcaaga aggtgatcat
     4801 accgcggtca aacctggacg atgtccttat tgaggatcgc taccgctcga tgatcgagat
     4861 tgtgccggtt gaccacatag aggaggttct ccagaacgcg ctcgtgcccg agaaccgtga
     4921 aggcttcctc tcgaaactcc ggaaactggc ggccaactcg gccggcggca tcctggaccc
     4981 gaacctcgga cacccggcgg cttgatcctg atgtctgacg ttcgctacta cgatatccgg
     5041 cgcgtacgcg gtgaggtcac ccggatcgat atcgacaacg gggtggtcga gtccgcagcc
     5101 acctcattct ttgatagaac ggttatccgg gcgcttgcat cgaacggctg gggtattctc
     5161 acccgggatc acgtcgacct cgactcaaaa cacgagatcg agaccctcgt cagggaggca
     5221 gcccgtcttg ccgccgtcac gaaagaccgc ctggaccttg cagacgcccc gcgcggcgca
     5281 ctccccatcc cccctctcgg agaggacccg cgggagatcg acatcgagga gaagaccagg
     5341 ctgcttgctg agatcgaggg ggcggcgcgg ctgcccgggg tgacgaacac acgggcgaac
     5401 tacaccgaag ggatcgactc ggtcaggttt cttgactctg aagggaacga atactcttat
     5461 cagtccgtcc gctccgggtt ctccgttgtc gcgatagcat ccagaaacgg ggttatgcag
     5521 atgggcagcg aacgcgaaca taccatcaga gggttcaacc tccggggcag acacgacctc
     5581 ggcaggaggg ctggcgaggt tgcggtctcg ctgctcgatg caaaaatccc gaagggcggg
     5641 atgcagcgtg ctgtcctcga cccggaactt gccggtgtct tcacccatga ggctgtcggc
     5701 cacgcgagcg agggcgacct cgttcgcgag ggggcgtcgg ttctcggcgg gaggatcggc
     5761 gagcatatcg gttgcgacgg gcttgtgata gttgacgatc cggcgctgca cgagttcggg
     5821 ttcatgccgg ttgacgccga gggcgtggcg gtgcagcgga cggagatcat ccgtgacggt
     5881 atactcgcat cctatctcca caaccgcgag accctggcgg cggtcgggga tggcatgccc
     5941 ggccacgcgc gcgccgaaca cggggcgccg ccaatagtca ggatgagcaa caccttcatc
     6001 gagaacggcg acgccacgcg cgaggagatc ctggcagagt gccgggaggg catcctgctg
     6061 atcggttcga ggggcggcca ggtcgacccc ggccgcggtg tcttccagtt caacgcggag
     6121 tacggatacc tcattgaggg cggcgagacg accggtatgc tccgtgacgt ctcgctctcg
     6181 ggcgagatcc tcagaacgct ccacaacatc accctgatcg gggatgaccg gaggatgaat
     6241 ccgggctact gcggcaaagg cgggcaggtc gtgccggtca gtgacggtgc gccccatctc
     6301 ctgcttgagg acgcgaccat cggggggcgc ggcgaatgaa cggcgaagac ctgatagaga
     6361 tggttctccg ggaaggtgag cgcctggcag atgaggttga ggtcttctcc gcccgcgggc
     6421 agagcgtcag caccgagatc aagaggggga tcatcggggt cgccgaggag tcggaggcct
     6481 ggagcatggc tatccggaca gtcaggaacg ggcggatcgg gttctcgtcc accggcgacc
     6541 ctgaccgctg gagggactgc ctggatgcgg cgctcgcaag cggccgactc gccacaccac
     6601 agcggtggga gggattccct ggaccggctg ctttctcagg cgtcccctcc tccgcagaca
     6661 gaggcctcga agtcgcgatc gaggacgcag ccaggatggt cgatgacctc ctcgccggcg
     6721 ccgcggagca ccccgtggat gtcatcggag ggtctgcgga tctcgggcgg tcgcacctct
     6781 cgatcgctaa ctcaaacggc gtctcctacg agatggaacg gacggtggtg ggagtctccc
     6841 tggagacgat ccgggaacag tccaccggct acgagttcga cgcctcaccg ttccgcaggg
     6901 atatcgacgc ccgcgccgtc ggagagcggg cggcgatgct tgccgcacgc tctcttggcg
     6961 ggcgcgatat agagaccggg cgatacgaca tagtcctctc ccctgttgcg gccgcaagcc
     7021 tcctcggcca ggttctcgtc ccggcacttt ccgggcggaa cgtgaaggcc gggcgatcgc
     7081 ccctggcaga cagggtgggc gagcaggtct ttgacgggtc actatcagtc tacgacgacc
     7141 catttgcccc cgggccggga agcaccgcct gggatgctga aggtgttccc acccgacgcc
     7201 ttctcttcat cgagcagggt gtggtcaggg ggtttgccta cgacctcaag accggctacc
     7261 gctacggtga ggagagcact ggaagcgccg tccgcggcgg gaacgaaccc ccggcgatcg
     7321 gggtgcacaa cctcttcatc aacggggccc gggagaaaga acctggttat gggcgtgcag
     7381 tctggatcca cgacgttgtc ggggcccaca ccgcaaaccc attctccggc gatttctcgg
     7441 ttgaggtctc aaaccccttc tggatggagg atggggaact gatcgaaccg atccgggcgg
     7501 cgatgctgtc aggaaacgtc tttgagatgc tcggagagat cgtggggttc gggaacgatg
     7561 caaggcgcgt cggacggctc accattccat caatacggtt aaataaccag cagatcattg
     7621 gtaaatagat gatggaagag atctttgcca tccttcttgc agttgcgata gccgcagcgc
     7681 tttactacct gatgaagaag gcgctgacgc ttgtcatcaa cgccatcgcc ggactcatca
     7741 cgctctgggc cctgaactac ttcagcgtcc tcgcctggtt tggcgcgccc gatatccaga
     7801 tctcgctcgt cacaatcctc atatgcgctc ttggagggct gcccggcgcc ctcatcctgg
     7861 tgctccttca ccttatcggg atcacgatct gagagcggct ttcctgcagc ctccagacgg
     7921 cacaccgcag gtcaaaagag gcttgcctcc gagtggacga ggataccgtc ctggaggatc
     7981 tcaaacggtt tgatctcacg cgaatgagcc gagcggcgca tcttcacgac ctcaagggcg
     8041 aggtggacct cggtgagccc cggagagcgg atgtagcgca gaagtatcac cgtatcggcc
     8101 aggtactcga tgatgccgta cctgctctcc tccgggtttc cgggacaggt ctcactcgtc
     8161 agcaccaggg tgcaggcctc atcccgcatc acctccacaa acctgaacat ctcccgccgc
     8221 cgctcggact catcctcaaa aagcccctca aacagggata tcgggtcgat gaccactctt
     8281 gaagcccccg tgctctcgat gagagccggg agttcgctct tgatgctgtt gatcgcaagg
     8341 ttgaaatcag tcggatcaag tttgaggagg taaaatcggt cgccaaacgc cccgggatcc
     8401 catcctttct gcgccatcgt ggcgcggagg agttcctccc gctcttcaag gctgatgtag
     8461 atcaccttct cgccgcggga gagaccctca taggcgaact ggagtgcaag catcgtcttc
     8521 ccggtgccgt acgtcccgac aagcgcacat atgctattct caataagccc ccccgagagc
     8581 atcgtgtcga gccccacaac accgaacctc gcccttagcg ccgttgttat accaccatcc
     8641 tgatgttcct gacctcaaag cctcccttcg ccgagataga gaccgcaaac tttaccaggt
     8701 tcttatcctc aagcctggtc atcacgcccc ggaacttccc aaagtacatg acccgctgcc
     8761 ggcgctgtgc gccgctctcc tcccactgga aaatgaacgt ggcatcgaca cagtcagcaa
     8821 tctcgagttc ccgcgaccta tcaa
//
LOCUS       DAOSZX010000059         8710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6876_length_8710_cov_19.893588, whole genome shotgun sequence.
ACCESSION   DAOSZX010000059 DAOSZX010000000
VERSION     DAOSZX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_8710_cov_19.893588"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PK196_05815"
     CDS             complement(<1..1724)
                     /locus_tag="PK196_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HON40969.1"
                     /translation="MRIAVVHRDRCHPVKCGTECILYCPRVRTGDETVIIGEDQKAVI
                     SEELCVGCGICVKKCPFDAIDIVNLPEHLDQPIHRYGRNGFVLYGLPIPIEGKVTGIL
                     GPNGIGKSTSVKILSGMLVPNLGRSDEEVSWDDVLDLYQGTELFDYLKLLSQKSVKVA
                     VKPQYVDQIPRVFSGSVRELLATTDERGRLDYYLGRLSLTNLLDRPITTLSGGELQRV
                     ALAACFARDADFYFLDEITPYLDVYQRMAAANLVRELAMERPVVIVEHDLAILDMLAD
                     TVHIAYGEPAVFGVITHPKGVRVGINQYLEGFLPEENVRFRAYAVAFETRAHTAAANR
                     EVLLEFPAMTKRFDRFSLTVEGGEIRKGEVLGVLGANGIGKSTFAGLLAGVIEPDTGA
                     MDKRVRISYKPQYLKGDTEATVEETLRQATTKFDTSYYQHEILEPLSLSQLLQAPVNT
                     LSGGELQRVAIAVCLSRNADLYILDEPSAHLDVEQRVRISRMIRKHAEAREASTLVID
                     HDIYVIDMISDRILVFEGNPGAEGRAAGPFDMAPGMNRFLKALGITFRRDKSGRPRIN
                     KPGSFLDR"
     gene            complement(1731..2096)
                     /locus_tag="PK196_05820"
     CDS             complement(1731..2096)
                     /locus_tag="PK196_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40970.1"
                     /translation="MSEKPGADEADKIRPQTRIEKQVEHRDRIKRTLVACFMGILVGG
                     LSFYLSGTPDPATGLQENAIIGILLLAAGVVFQKYVFMFIGVDYMELSTKDWFYQSFM
                     TFALWFMTWTIFLTTTVLL"
     assembly_gap    2117..2216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2218..3246)
                     /locus_tag="PK196_05825"
     CDS             complement(2218..3246)
                     /locus_tag="PK196_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HON40971.1"
                     /translation="MKAEVREMKKQFGEIRLFPETLDDLWHLSHLIRPGDLVFATTFR
                     GVETATDKIRPEKVEKRPVRLGIRVEKVEFHQHTNRLRVSGVIESGVDVSAHHTLNIE
                     TGFEISVIKRWRAVDCERIRRAVAASEYGVVHVVSIEEGEAQVYRLRQFGPEWVTTVT
                     VGSSKGAGTGSSRSALFEKTLEAVSAVTGPLVVAGPGFVKDEFVDYVKTRDPDLAGRT
                     VTVETRRIGRGAVQEVIGQGVIERLLGDLHLGREVTLMEEVLLRIATGGAVAYGIEDV
                     RKAVTYGAAETVLVADTLLRDEEAATLIEQAEQINTTIVVMSTAFEPGERLEAIGGMA
                     ALLRYKIE"
     gene            complement(3243..5141)
                     /gene="rqcH"
                     /locus_tag="PK196_05830"
     CDS             complement(3243..5141)
                     /gene="rqcH"
                     /locus_tag="PK196_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HON40972.1"
                     /translation="MATLQGMSGIDLRALVGEASERLPLWVGKIYQFDAKTLGIRLNG
                     EDRAKYQLLAEAGRRVHFTAEFPTPPKHPPGFAMLLRKHLDGGKVLGIHQFGLERAIC
                     LDIGKRETTYHLIFELFDEGNVVLCDEGYTIIKPLWHHRFRSREVVPGAVYATGGPDC
                     STLSPDEFRAMLAASDRDIVRTLAVDCLLGGAYAEAVCRMAGVDKSAPAADVDAGAVR
                     EALGHLIADVEGHREPVITATGCWPVVLPGEEVIERFSTFSEALDKFYPPAAGEKEET
                     APGKPRLSREEVIRQRQAEAIRGFEKKIERYERVVEAIYENYTTVAGILATLDEASRT
                     RSWQEIEAILRSNRGNPAAKMVCAVHPAEAAVELDLQGERVKVYVHETIEQNLGRCYD
                     QIKKFKKKRAGAVAAMERAVPERPRPKRDIPLQKKRWYHRFRWFFTSDGVLVIGGRDA
                     SQNEELVRKYMEGGDLFVHADVHGGSVVIVKGRTEHLDEAAQFAASYSNAWKAGHFTA
                     DVYAARPEQVSKTAESGEYVARGAFVVRGERQYFRDVPLGVAIGLQVSPEVAVIGGPP
                     AAVAGRARAWVELRPGQFEPNDTAKKVVRALRDKLPEDEWKGLKNALNTEAVAAFVPP
                     GGSDLIES"
     assembly_gap    5546..5645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5646..6038)
                     /locus_tag="PK196_05835"
     CDS             complement(<5646..6038)
                     /locus_tag="PK196_05835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON40973.1"
                     /translation="MALQEGDFVRIRYTGRIGGDVFDTTDEEIAKEEDVYNQMAEYGP
                     VTIRLGSRHILAGLEEELIGKEVGTEGEVEIPPEKAFGEHDENEVMSFPVTQFREKPE
                     PGMRVSIEGREGMVISVIGRRAVVDFNHP"
     gene            6189..6716
                     /gene="cyaB"
                     /locus_tag="PK196_05840"
     CDS             6189..6716
                     /gene="cyaB"
                     /locus_tag="PK196_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HON40974.1"
                     /translation="MLEVEAKFAVRDLDAIRSRLESMGARKGRRQQEQDVYYNAPHRD
                     FGETDEALRVRYDDTGVTLTYKGPKVRVGGAKAREEHNLAVNSGETLKAILSCLGFRR
                     AATVSKTREFYYMGDATVTLDTVEGLGTFIEIEILTEDGRDDAAARIGAIAKEVGVDG
                     PPIYTSYLEMLLFKR"
     gene            complement(6706..7347)
                     /locus_tag="PK196_05845"
     CDS             complement(6706..7347)
                     /locus_tag="PK196_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HON40975.1"
                     /translation="MMDVLLLADLHGNYGKVDAFLGYDYDAVIIAGDITNFGPLEPVE
                     SLLSSFEVPCFAIPGNCDPREIVEVLERSDAVCLHNSWISIGKVTFAGLGGSNKTPFD
                     TPFELTEEEIDKELGRIVRYMERNVHNVLISHAPPYKTLDAVGDNHVGSQSIRKHMTG
                     FDLVCCAHIHEARGITKADGVTIVNPGPASDGYGALINFGNEPKEIRIDLISV"
     gene            complement(7351..8463)
                     /locus_tag="PK196_05850"
     CDS             complement(7351..8463)
                     /locus_tag="PK196_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HON40976.1"
                     /translation="MRVYWRYIPQARNTLAALAAACEVHGYELDVADGPRPDVTIYSL
                     NSINERVYRDEIAGADCITIVGGPHASACYREVAEYADYVVVGEGEYTLPALLAAIED
                     GRNAPPGVATSAGYTPAQHTVLLDGYPPFSRIKGFVEITRGCPFSCGYCQTPRLFGRC
                     MRHRSIDEIVRYASRYRDIRFVAPNAFAYGSDGIHLRLDRVERLLRQLDGRRVFFGTF
                     PGEVRPECVSRESLELVTGHCANTRLHFGAQSGSDAVLRRLHRGHTVQDVLSAVDLCR
                     EYGLVPVVDFILGLPFETDDDQRATLDLVREVTRAGKAHIHYFMPLPGTPLSNARPRP
                     LLPETESVLGKLALDGRITGSWMDHEIRFFRHTSHL"
     gene            complement(8502..>8710)
                     /locus_tag="PK196_05855"
     CDS             complement(8502..>8710)
                     /locus_tag="PK196_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HON40977.1"
                     /translation="TLMDWKGLRVYLKLKDCATSGKKLEAYKREVAAALGEDAAKMMH
                     ATLTFEVTEVRPIVDMGQGWEKSV"
BASE COUNT         1589 a         2681 c         2519 g         1721 t
ORIGIN      
        1 tcgcggtcaa ggaacgatcc cggcttgttg atccgcggcc tcccgctctt atcccggcgg
       61 aacgtgatcc cgagcgcctt aaggaacctg ttcatgcccg gcgccatatc aaacggcccg
      121 gcggccctgc cctccgcccc ggggttgccc tcaaagacca ggatcctgtc gctgatcata
      181 tcgatgacgt agatgtcgtg atcgatcacg agcgtgctcg cctcccgggc ctcggcgtgc
      241 ttacggatca tccgggagat cctcacccgc tgctccacgt ccaggtgggc gctcggctcg
      301 tcaagtatgt agaggtcggc gttgcgggag aggcagactg ctatcgccac ccgctggagt
      361 tcgccgccgg agagggtgtt caccggcgcc tggagcagtt gcgagaggga gagaggctcc
      421 aggatctcgt gctggtagta ggatgtatca aacttcgtcg tcgcctgccg gagggtctcc
      481 tcgaccgtcg cctccgtatc ccccttgagg tactgcggct tgtaggatat ccgcaccctt
      541 ttgtccatcg ccccggtgtc gggctcgatc accccggcca ggagccctgc aaacgtgctc
      601 ttccctatac cgtttgcccc gagcactccc aggacctccc ctttgcggat ctcgccgccc
      661 tcaaccgtta gtgagaaccg gtcgaaccgt ttggtcatcg ccgggaactc aagcagcacc
      721 tcccggttgg ccgcggcggt gtgcgcccgg gtttcaaacg ccacggcgta tgcgcggaac
      781 ctgacgttct cctccggcag gaacccctca agatactggt tgatcccgac ccggacaccc
      841 ttcgggtggg tgatgacacc aaagaccgcc ggttcaccgt aggcgatgtg cacggtgtcg
      901 gcgagcatat cgagtatggc aaggtcgtgc tcgactatca caaccgggcg ctccatcgcg
      961 agctcccgga caaggttcgc ggcagccatc cgctggtaga cgtcaagata cggtgtgatc
     1021 tcatccagga agtagaaatc agcatcgcgg gcgaaacagg cggcaagcgc cacccgctgg
     1081 agttcgccgc cggagagggt cgtgatcggc cggtcgagga gattcgtgag cgacagccgc
     1141 ccgagataat agtccagtct gccgcgctcg tcggtcgtcg ccaggagttc acgaaccgaa
     1201 cccgagaaga ccctcgggat ctggtcaacg tactgcggct tcaccgcaac cttcacactc
     1261 ttctgcgaga gcagtttgag gtagtcgaag agttcggtcc cctggtagag gtcgaggacg
     1321 tcgtcccagg agacctcttc atcgctcctc ccgaggtttg gaaccagcat cccggagagt
     1381 atcttgaccg atgtgctctt ccctataccg tttggcccga ggatgcccgt gaccttaccc
     1441 tcgatcggta tcgggaggcc gtagaggacg aacccgttcc tgccgtagcg gtggatcggc
     1501 tggtcaaggt gttcggggag gttgactata tcgatggcat caaaagggca cttcttcacg
     1561 cagatgccgc agccaacaca gagctcctcg gatatgacgg ctttctggtc ttcgcctatg
     1621 atgacggtct catcccccgt cctgacgcgc gggcagtaga ggatgcactc tgtcccgcac
     1681 ttgacaggat ggcatctatc gcggtgtaca acggcaatac gcatgggtta tcacagaagg
     1741 acagttgtcg taaggaagat cgtccaggtc ataaaccaga gtgcaaacgt cataaaactc
     1801 tggtagaacc aatctttggt ggagagttcc atgtaatcga cgccgatgaa catgaagaca
     1861 tacttctgga agacaacgcc cgctgcaagg agaagtatac cgatgatagc gttctcctga
     1921 agcccggtcg ccgggtcggg cgtgcctgag aggtagaatg agagaccccc gaccaggatg
     1981 cccatgaagc aggctacaag cgtccttttt atccgatccc tgtgctcgac ctgtttctcg
     2041 atacgggtct gcggccgtat cttatcggcc tcgtctgctc ccggtttttc gctcatcgtg
     2101 acacccgtgc tgcgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca
     2221 ctcgatcttg tagcgcagga gcgccgccat accaccgatc gcctccaggc gctctcccgg
     2281 ctcaaacgcg gtgctcataa ccactatcgt ggtgttgatc tgctcggcct gttcgatgag
     2341 tgttgcggcc tcctcatccc ggagcagggt gtcggcgacc agcaccgtct ccgcggcgcc
     2401 gtatgtgacc gctttgcgga catcctctat gccgtaggcg acggcgcccc cggtggcgat
     2461 ccggagcagg acctcctcca tcagcgtgac ctcccttcca aggtggagat cgccgagcag
     2521 cctctcgatg acgccctggc cgatgacctc ctggacggcg ccccgcccga tccggcgtgt
     2581 ctcgacggtg accgtccgcc cggcgaggtc gggatcgcgg gtttttacat aatcgacgaa
     2641 ctcgtccttc acaaacccgg gccccgcgac gacaagaggc ccggtgaccg ctgagaccgc
     2701 ctcgagcgtc ttctcaaaca gcgccgatcg gctgctgccc gtcccggcgc ccttgctgct
     2761 gccgactgtc acggtggtca cccactccgg gccgaactgg cggaggcggt agacctgggc
     2821 ctcgccctcc tcgatgctca cgacgtgaac cacaccgtac tcggaggcgg cgacggcgcg
     2881 ccggatccgt tcacagtcga cggcgcgcca gcgttttatg accgagatct caaacccagt
     2941 ctcgatattc agtgtatggt gtgcggatac gtccaccccg ctctcgatga cgccggagac
     3001 cctgagacgg tttgtgtgct ggtggaactc caccttctcg acgcggatcc cgagcctgac
     3061 cggtcgtttc tcgaccttct cggggcggat cttatcggtc gccgtctcca cgccgcggaa
     3121 tgtcgtcgca aagacgagat cccccggccg gatcaggtgg gagaggtgcc agaggtcgtc
     3181 gagcgtctca ggaaagagac ggatctcgcc aaactgcttc ttcatctcac ggacttcggc
     3241 cttcatgact ctatgagatc ggatcccccc ggcgggacaa acgcagcaac cgcctcggtg
     3301 ttcagggcgt tcttgagccc tttccactcg tcctccggga gtttgtccct gagcgcccgg
     3361 acaaccttct ttgccgtgtc gttgggctca aactgccctg gacggagttc aacccacgcc
     3421 ctggcacgcc cggcgacggc agcgggcgga ccgccgatga cggcgacctc cggcgagacc
     3481 tgaagaccga tggccacacc gagggggacg tccctgaagt actgccgttc gccccggacg
     3541 acgaaagcgc ctctcgccac gtactccccg gactcggcgg tcttgctaac ctgttcgggc
     3601 ctggcggcat agacgtcggc ggtgaaatgc ccggccttcc aggcgtttga gtaggatgcg
     3661 gcgaactgcg cggcctcgtc cagatgttct gtcctgccct tcacgatgac cacgctcccg
     3721 ccatggacat cggcgtggac gaagaggtcg ccgccctcca tgtacttcct gacgagttcc
     3781 tcgttctggg aggcgtcccg gccgccgatg acaaggacgc cgtcgctggt gaagaaccag
     3841 cggaaccggt ggtaccaccg cttcttctgg agagggatat cccgtttcgg ccgcggtctc
     3901 tccgggaccg cccgctccat cgccgcaact gcacccgccc tcttcttctt gaacttcttg
     3961 atctggtcgt agcagcggcc gaggttctgc tcgatagtct cgtggacgta gaccttcacc
     4021 cgctccccct ggaggtcgag ttccaccgct gcctcggcgg gatgcaccgc gcagaccatc
     4081 ttcgctgccg ggttgccgcg gtttgacctg agtatagcct cgatctcctg ccaggaacgg
     4141 gttctgcttg cctcatcgag cgtcgccagg atcccggcaa ccgttgtgta gttctcgtag
     4201 atcgcctcca ctacgcgttc gtagcgctca atcttcttct caaaccccct gatcgcctcg
     4261 gcctggcgct gccggatcac ctcttcccgg gagaggcggg gttttccggg tgcagtctct
     4321 tctttctcgc cggctgccgg cgggtagaac ttatccagcg cctcgctgaa tgtcgagaac
     4381 ctctcgataa cctcttctcc tggtagcacc accggccagc atcccgtggc cgtgatcaca
     4441 ggctcccggt ggccttcgac atcggctatg aggtgtccga gcgcctccct gacagccccg
     4501 gcatcgacat ctgcagcagg ggcgctctta tcaacccctg ccatccggca gaccgcttcg
     4561 gcgtatgcgc cgccgaggag gcagtcgaca gcgagcgtcc ggacgatatc gcggtcggac
     4621 gccgcaagca tcgcccggaa ctcatcgggg gagagggttg agcagtcagg tcccccggtg
     4681 gcatataccg cgccggggac cacctcccgg ctcctgaacc ggtggtgcca gagcggtttg
     4741 atgatggtat acccttcgtc gcagaggaca acgttcccct catcaaacaa ctcaaagatg
     4801 aggtgatagg tcgtctccct cttccctata tcgaggcata tcgcgcgttc aagcccgaac
     4861 tgatgtatgc caagcacctt tccgccgtcc aggtgctttc gaagcagcat cgcaaacccg
     4921 ggagggtgtt ttgggggtgt cgggaactcc gcggtaaaat gaacccgtct cccggcctcc
     4981 gcaagcaact ggtatttcgc ccggtcctcg ccgttcaacc tgatgcccag ggtctttgcg
     5041 tcgaactggt agatcttgcc gacccagaga gggaggcgtt cggaggcctc cccaacgaga
     5101 gcacggaggt cgatcccgct cataccctgt agtgtcgcca ttgattcaga gtacatacgg
     5161 cccgccacct aaataaggac acggccgggc cgggaggcag gggttgccaa aacccgtgcc
     5221 ttcagaccgg gctctggttt gatgaaccct gccatggacg ggctacgggt tcgggggtct
     5281 accccctccc ggcgaagaga gtgtcctggc gacagggtga aacgtgggcg gggacgtccc
     5341 gctggcgata ctccatcgac gcagtgtccg tggaggcgat aatccctgca ttcctgttcc
     5401 aggcacggcc ccccgccgat caccgcgcac ttgccaaaac ccccctcgca ctttggtcgg
     5461 cgaaagaggt tcaacacgag tgatccgggg cgccggggaa ctggtttggc aactcccccg
     5521 cttaatacct ggaaacgtgg agagtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnngggat ggttgaagtc aacgaccgcg cgcctcccta tgacgctgat gaccataccc
     5701 tcgcggcctt cgatgctgac ccgcatcccg ggttcgggct tctcgcggaa ctgcgtgacc
     5761 gggaaagaca tcacctcgtt ctcgtcgtgc tctccgaacg ccttctctgg cgggatctcg
     5821 acctctcctt ccgtgccaac ctcctttccg atcagctcct cttcaagacc ggcaaggatg
     5881 tggcggcttc caaggcggat ggttaccggg ccgtattccg ccatctgatt gtagacatcc
     5941 tcttcctttg caatctcctc atctgtggta tcaaaaacgt ctccaccgat acggccggtg
     6001 tatctgatcc tgacaaaatc tccttcctga agtgccattt ccattcacca tggttagttg
     6061 gtgcgcagca gtctcccgct gcggcattcc cgcagcggcg caccgcgcac ccctgtcaca
     6121 ttgctccatt tcattaggat ggaatggtat ttatcatcat tccagagagt ctatctgatg
     6181 cgagggttat gctagaggtg gaggcaaagt ttgcggtccg ggatctcgat gccatcaggt
     6241 cacggctaga gagtatggga gcgcgaaagg gcaggagaca acaggagcag gacgtctact
     6301 acaacgctcc gcaccgtgat ttcggagaga ccgatgaagc gctgcgggtc aggtatgatg
     6361 ataccggagt caccctgacc tacaaaggcc cgaaggttcg tgttggcggc gcaaaggccc
     6421 gggaagagca taacctggcg gtgaactccg gggagaccct caaggcgatc ttatcctgcc
     6481 tggggttccg gcgcgcagcc accgtctcca agacgcgcga gttctactat atgggagatg
     6541 cgactgtcac actcgacacc gtcgaggggc tcgggacgtt catcgagatc gagatcctga
     6601 ccgaagacgg tagagacgat gccgccgcaa ggattggtgc gattgcaaaa gaggtgggtg
     6661 tagatggtcc accgatctac acctcgtatc ttgagatgct gctgttcaaa cgctgatcag
     6721 atcaatacgg atctcttttg gctcattccc gaaattgatg agagcgccgt atccatccga
     6781 tgcaggtccg gggtttacaa tggtgacgcc gtctgccttg gtgatccccc gtgcctcatg
     6841 gatgtgggcg cagcagacca ggtcgaaacc ggtcatgtgc tttcttatgc tctggctgcc
     6901 gacgtgatta tcgccgacgg catccagcgt cttgtatggc ggggcatggc tgatcaggac
     6961 gttgtgaacg ttccgctcca tgtacctgac aatccgcccc agttctttat caatctcttc
     7021 ttccgtcagt tcaaacggtg tgtcaaaggg ggtcttgttc gagcccccca ggccggcgaa
     7081 tgttaccttc ccaatggaga tccaggagtt atgcaggcag accgcatccg aacgctcgag
     7141 cacctcgacg atctcgcggg gatcgcagtt gcctggtatt gcaaaacatg gcacctcgaa
     7201 ggaggagagt aatgactcta caggttcaag tggaccgaaa ttggttatgt caccagcgat
     7261 tatgaccgca tcgtagtcgt agccgagaaa agcgtcaact tttccgtagt ttccgtgcag
     7321 atctgctagg aggagcacat ccatcatgct ctatagatga gaagtgtgtc taaaaaacct
     7381 tatctcatga tccatccacg agccggttat tctgccatcg agcgccagtt ttccaaggac
     7441 gctctcggtc tccggaagga gcggccgggg gcgggcgttt gagagcggtg tccccggcag
     7501 cggcatgaag tagtggatat gcgccttccc ggcccgcgtc acctccctca ccaggtcaag
     7561 cgttgcgcgc tggtcatcat cggtctcaaa tggcagcccc aggatgaaat caacgaccgg
     7621 aacgagcccg tattcgcggc agaggtcaac ggcggaaagg acgtcctgga ccgtatgccc
     7681 ccggtgcaga cggcgcagca ccgcatcact ccccgactgc gccccaaagt gaagacgggt
     7741 gttggcgcag tggccggtca cgagttcaag ggactctcgc gagacgcact ccgggcgcac
     7801 ctcgcccggg aatgtgccaa agaagacccg ccgaccatcg agttgccgga ggagccgttc
     7861 taccctgtca agccgcagat ggatgccgtc agagccgtag gcaaaggcgt tcggggctac
     7921 gaaccggata tcccggtagc gcgaggcgta gcggacgatc tcgtcgatgg agcggtggcg
     7981 catacaccgc ccgaagaggc gcggcgtctg gcagtagccg caggagaatg ggcatcccct
     8041 cgttatctcg acaaaaccct tgatacgcga gaagggcggg taaccgtcga ggagaacggt
     8101 gtgttgtgca ggggtgtagc ccgcagaggt ggcaacccct ggaggagcgt tccgcccatc
     8161 ctcgatggcg gcaagaagcg ccgggagggt gtactcgccc tccccgacga cgacgtaatc
     8221 ggcgtattcg gccacctccc ggtagcaggc ggaggcatga ggcccgccga caatcgttat
     8281 acagtctgcg ccggcgattt catcacggta gacgcgctcg ttgatagagt tcaggctgta
     8341 gatggtgacg tccggccggg ggccgtccgc cacatcgagt tcgtagccgt gcacctcgca
     8401 cgccgcagca agcgcggcga gggtgttcct cgcctggggg atgtatcgcc agtataccct
     8461 catatcacgg gttttgcggg cgtgccgggt gctcaaagcg ttcagactga cttctcccag
     8521 ccctggccca tgtcaacgat cgggcggacc tccgtgacct caaacgtcag cgtcgcgtgc
     8581 atcatcttcg ccgcatcctc gcccagtgcc gctgcgacct ccctcttgta ggcctcaagc
     8641 ttcttacctg aggtcgcgca gtccttcagt ttcaggtaga cccgcagacc cttccagtcc
     8701 atgagggtct
//
LOCUS       DAOSZX010000060         8662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_6935_length_8662_cov_21.453003, whole genome shotgun sequence.
ACCESSION   DAOSZX010000060 DAOSZX010000000
VERSION     DAOSZX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6935_length_8662_cov_21.453003"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..463)
                     /locus_tag="PK196_05860"
     CDS             complement(77..463)
                     /locus_tag="PK196_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HON40978.1"
                     /translation="MEKVKRYRCKYCNYIYSPLRGEPHRGIPEGTAFEDLPDDYVCPV
                     CGATGKGPVGKWGFEPWEPTKYVCKICGYIYDKKRGEPLRGIPAGTAFEELPDDYVCP
                     VCGLDPKISSHYGPVGKTQFEPILDV"
     gene            complement(1236..1637)
                     /locus_tag="PK196_05865"
     CDS             complement(1236..1637)
                     /locus_tag="PK196_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HON40979.1"
                     /translation="MQGRGISRGRGTGELLVSSEPISFLSGVDPETGVVVERGHPLEG
                     QSVTGKVLVFPHGKGSTVGSYVIYALKQNGVAPAAIINQEAEPIIAVGAIIAGIPMVD
                     RLPPEFYKLPPGRKVTVNGDTGEVCLDGETG"
     gene            1691..2860
                     /locus_tag="PK196_05870"
     CDS             1691..2860
                     /locus_tag="PK196_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HON40980.1"
                     /translation="MDETIGNTSSSSSEHDMLTLQIQELKAQVLDYKLKNDLLEKEVR
                     QLRKENAQLKRVPLFVAAVVDVLENGEVYLRQQGNNQEYLTVVNDKLHSTLKPGMKVA
                     VNNTLSIVKIIGNIYDARVRVMELDEQPNVTFDQIGGLREEIEEVREAVEYPLTKPEI
                     YKRIGVEPPKGILLYGPPGTGKTLIAKAVARQSKACFIRMSGSELVHKYIGEGAQLVR
                     ELFTLARERAPAIVFIDEIDAIGSMRTNDGTSGSAEVQRTLMQLLAEMDGFGNRGNIR
                     IMAATNRIDMLDPALLRPGRFDRIIEIPLPDAEARIEILKIHSAKMTLSRNVDLPVVA
                     ELAGKTTGADLQAICREAGMMAIRRNADAVGQEDFIAAIRKVKREAPAPDSRMYI"
     gene            3107..3595
                     /locus_tag="PK196_05875"
     CDS             3107..3595
                     /locus_tag="PK196_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HON40981.1"
                     /translation="MKLEMKDQPGQLVAALQPISAVGGNIIAVIHQREPHAATEVLDV
                     QIVLELPEGRLEKLLALLRQQGVKVVRIGEERLLYECTLIMIGHLMHTDISDTVDQID
                     TTGSAEVTELSLVMPAIDSPSSARITIRSTTRTEMERAIRILRRVAEQKNILIIEPLE
                     SV"
     gene            3595..4581
                     /locus_tag="PK196_05880"
     CDS             3595..4581
                     /locus_tag="PK196_05880"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HON40982.1"
                     /translation="MRIAILGFGSVGRGIARVILAKNLDILVTGLADSRSGIIDPAGI
                     DIEAALARKEEGAPCGSPDIRPIDVVTGADYDILVEVTPTNVENGGPALNHIRTALQR
                     KKHVVTSNKGPIALAYPELRALAEENGVSLRYEATVCGAIPLIHAMQEGLAGNTISRL
                     YGVFNGTCNYILTRMAAEGLTYAQALAEARELGYAEADPTYDVEGIDAAIKLVILANT
                     VLDMQTTLDRVDRTGISLLTPEALHLAEEQDCTIRLIGEIVPAAGALRVSPRIITKTH
                     PLVVEGTLNAVTLETDLAGDLTFIGKGAGSIETASAVIGDILYIRDRYVQGS"
     gene            4565..5695
                     /locus_tag="PK196_05885"
     CDS             4565..5695
                     /locus_tag="PK196_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON40983.1"
                     /translation="MSRVLERRKQLMRLMRQATLDNGYFTVAGIAEATGIPRSTIQDW
                     VNRLIEEGCVALLEEQRGRHAARYVASSVMPESACRRVFTTIDGEEVEIYHECMSGGC
                     AAFCEFHHARAGGALQSVWRDGTLLRERAHLGRQEVAVGLDPAPAVGIIGVFHEDGRI
                     RQQIRCIGGPAYSLTDMMSFAEGVCGVTVHREGPLVEGEVVTRALAYVAIGIDDTDTA
                     AEGATFALALALLQHLTKLDGVMPIGHRVAMLNPHLEPRTAGNSCSCIEVAVEPSMIP
                     RIEEAAVRFVAGEAASPEWGIALREGFVVPRDLRAYGKGAREAVIEREEAEATAERFG
                     AHLHGGRGVIGALAAVSLIGLPHEVLLDPGMDVCTDRDPEHQ"
     gene            5840..6487
                     /locus_tag="PK196_05890"
     CDS             5840..6487
                     /locus_tag="PK196_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HON40984.1"
                     /translation="MRVELEEEIEKLSSLARERGAEPRRIAAHEIVVAEWVRFKCRYG
                     CKGYGKHMSCPPYAPAPAETRQMIGEYSTGLLLKFEGIPGHPDLKPEEIPLDFHPFLR
                     DLILWVNSTVHFLEKTAFYDDFPKAFGFGGYPCIYCEHLHCVAEEGEGVVDESIRRLC
                     RHMDLVRPSMEAAGIDVFSTARRAGWDIHIVPCRNLEYGKIEHGNIRSVGLVLIE"
     gene            complement(6709..7812)
                     /locus_tag="PK196_05895"
     CDS             complement(6709..7812)
                     /locus_tag="PK196_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HON40985.1"
                     /translation="MDSIRIAIVGVGNCASSLLQGIEYYRGKNEKDTTGLMHWDLGGY
                     RPFDIEVAAAFDIDARKVGKDVSEAIFSPPNCTMVFCPDVPRTGVTVRMGRVLDGFPE
                     HMLDYKEDCRFVLADEEEASREDIVRELEDSGAEILLNYLPVGSEKAARFYAECALEA
                     GVGFINNMPVFIASDPAWAERFQERGLPIIGDDIKSQIGATITHRVLADLFRQRGVVL
                     ERTYQLNTGGNTDFLNMLNRDRLASKRRSKTEAVQSVLKEPLDADNIHIGPSDYVCWQ
                     KDNKVCFLRMEGRIFGDVPMYLDLRLSVEDSPNSGGIVIDAIRCCRLALDRGIGGPLI
                     SPSAYFMKHPPVQFRDDDAYHMTEEFIQGIRDS"
     gene            8137..8640
                     /gene="fae"
                     /locus_tag="PK196_05900"
     CDS             8137..8640
                     /gene="fae"
                     /locus_tag="PK196_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HON40986.1"
                     /translation="MVDFEHALIGEALVGEGPEVAHIDLVIGKKGSSVEQAFVTALAS
                     PVKGHTPLLAVLTPNVPAKPSTLMVNKVTIKNAAQALLIFGPAQAAVAKAVVDSVAEG
                     IIPEAEADNLLIIASVFIEWDAKDKKKIYDWNYEATKTAIHRAIRGEPKMKDVLAQKD
                     TAKHPFA"
BASE COUNT         1866 a         2504 c         2562 g         1730 t
ORIGIN      
        1 aaaagatgct gcagcagacc gtaaggccgc accgacctcg ggcggcgagg tcgcgggggc
       61 ctgccagcct caaccttcag acatccagga tcggttcaaa ctgcgtcttg cctacaggtc
      121 cgtagtggct cgatatcttt gggtccagcc cgcatacggg acatacgtag tcgtcaggca
      181 gctcctcaaa cgcggttcca gccgggatgc cacgcagcgg ttcgccgcgc ttcttgtcgt
      241 agatgtaacc gcagatcttg cagacgtact tcgtcggttc ccagggctcg aatccccatt
      301 tccctaccgg accttttccg gtcgcaccgc agacaggaca tacatagtca tcaggcaggt
      361 cctcaaacgc ggtaccttca gggatgccgc ggtggggctc ccctctcaga ggggagtaga
      421 tgtagttgca gtacttgcag cggtatcgtt ttaccttctc catggtcata acgttcacct
      481 cgccgggtat ctcccggcca taaacggttc ctcaggaggt aggaaggcag ccccggcaga
      541 taaccttttc catggtccgg ttcaggaggc aggcagggca tcaaaccaac acctcaggcc
      601 gtggcacagg tccatgaact ttgcaaaaga actctaatct ggaggcgttt tcttctggtg
      661 tttacaaaca accgcctcat ctctgaattg tgtccgggcc tgaccaaccc cggtttgaga
      721 agcctctctg gacgactagc ggggggtttt ccatagaata cggtgccagg ggatctggca
      781 gccccttcgg ggtctcatct gacaatcctg agagatcctg cctctgtccc gtgcacggct
      841 atccagggtc aatcgccaga cactcgcccg ccccctctgg aggggggcgg aggccgcgaa
      901 gcggccgggc ggcgggggtg atccagcgag aggcattttt cagtgccacg ttgagacagg
      961 gaagggggaa gacgggtgga ggaagacagg acttcgatta tcggtaaacc tttatgagat
     1021 ctatgtttcg ccccccccga tacggccacc gggtcaattg ccgcacactg cccgccggcc
     1081 atcccgccgg gcggccctgg atagatagga tccggggggg cgcctcaaac gatcccaaaa
     1141 gcgcctgatt gctcttgcgt caggcaactc ccgctaccgc aaccactcaa aactgcccac
     1201 cggcaagaat caggtcagat aaaacgcttc aacccctatc cagtctcccc gtccaggcag
     1261 acctcccccg tatccccgtt caccgtcacc ttcctgccag gtgggagttt gtagaactcc
     1321 ggcggcagac ggtcgaccat cggtatcccg gcgatgatag cgccgaccgc gatgataggt
     1381 tcggcctcct ggttgattat agcggccggt gcaaccccgt tctgcttcaa cgcgtagatc
     1441 acgtatgacc ctaccgtcga tcccttgccg tggggaaaga ccagcacctt tccggtgacc
     1501 gactgaccct cgagcgggtg gccgcgctca accactacgc cggtctcggg gtcgacgccg
     1561 gagaggaagg atatgggctc agatgatact aggagttctc ctgttccgcg acccctggat
     1621 atgcctctgc cctgcatgat gacctgcacc acagtaataa tgtaggtgga gacttaacta
     1681 tatgattgat atggacgaaa ccattggcaa cacctccagc agcagcagcg agcacgatat
     1741 gctcacactc cagatccagg agttgaaggc gcaggtcctg gactacaagc tcaagaacga
     1801 tttgctggag aaagaggtcc ggcagctccg caaagaaaat gctcagttaa agcgggtgcc
     1861 gctgtttgtt gccgctgtgg tcgatgtgct cgagaacggc gaagtctacc tccggcagca
     1921 gggcaacaat caggagtacc tcacggtagt caacgataaa ctgcacagca ccctcaaacc
     1981 cgggatgaag gtggcggtga acaacaccct ctcgatcgtc aagattatcg gaaacatata
     2041 cgatgcgcgt gtcagggtca tggaactgga cgagcagcca aacgtgacgt ttgaccagat
     2101 cggagggctg agagaggaga tcgaagaggt ccgggaggcg gttgagtacc cgctcacaaa
     2161 acccgagatc tacaagcgta taggcgttga accgccaaag ggcatcctcc tctacggccc
     2221 acccgggacc gggaagaccc tgatcgccaa ggcggttgcc cgccagtcca aggcttgttt
     2281 catcaggatg tcgggaagcg aactcgtcca caagtacatc ggtgaaggcg ctcagctcgt
     2341 gcgcgaactt ttcacactcg ccagggagcg cgctccggcc atcgtcttca tcgacgagat
     2401 cgacgctata gggagcatgc gtacaaacga cggcacctcc ggcagtgccg aggtccagcg
     2461 gacgctgatg caactcctgg ccgagatgga cggcttcggg aaccgcggca acatcaggat
     2521 catggcggca acgaaccgga tcgatatgct tgaccccgcg ctcctccgcc ccgggcggtt
     2581 tgaccggatc atcgagatcc cgctcccgga tgccgaggcg cgcatagaga tcctgaagat
     2641 ccacagcgca aagatgacac tctcccggaa cgtggatctg cccgttgttg cggaactcgc
     2701 tggaaaaacc accggtgcag atctgcaggc gatctgccgg gaggccggga tgatggcaat
     2761 ccggcggaac gccgacgccg tcggccagga agatttcatc gcggctatcc ggaaagtgaa
     2821 gcgcgaggcg ccagcgccag actcccggat gtacatataa tctgggccat ccccccgatt
     2881 ttttcacccc actgatccag ccggcgtgtg atttatcctg ctggggtgaa gtaggcggag
     2941 cgggaagttc ccgcccggaa ggtgcgaggt agttcaccgc gctcgatgca atggtccagt
     3001 cgtaatggaa agtggtatca ccaccacacg agggcctgct gtgtggtaga aggattatct
     3061 ccacggcaga tcaacaattc agcaggtggt ttttttgcgg gtatccatga aactcgagat
     3121 gaaagaccag cctggccagc tggtcgcagc cctccagccg atctcagcag tcggcggaaa
     3181 catcatcgcg gtcattcacc agagagaacc ccacgcggct acagaggtgc ttgatgtaca
     3241 gatcgtgctg gaacttccgg aggggcgcct cgagaaactt cttgccctgc taaggcagca
     3301 gggtgtcaaa gtcgtgcgta taggggaaga acgcctcctc tacgagtgta cgctgatcat
     3361 gatcgggcac ctgatgcaca cggacatatc cgacacggtc gaccagatcg acacaacagg
     3421 ttcggccgag gtgacggaac tctcgcttgt catgccggcg atcgactcgc catcgtctgc
     3481 gcgcatcacc atccgttcaa caacccggac ggagatggag agggcaatcc ggatcctgcg
     3541 cagggtcgcg gagcagaaga acattctcat aatcgagcct ctggagagcg tatgatacgg
     3601 atcgccatac tcggcttcgg gtccgtcggt cgcggcatag cccgtgtgat acttgcaaaa
     3661 aaccttgata tccttgtcac cggtcttgct gactcacgaa gtggaataat cgatcctgcc
     3721 gggatcgaca ttgaagcagc acttgcccga aaggaagagg gtgctccatg tggcagtccg
     3781 gatattcggc cgatagatgt ggtgaccggg gcagactatg atatcctggt agaggtgaca
     3841 cctacaaacg tggagaacgg tgggcctgca ctcaaccata tccggacggc tttgcagcgg
     3901 aagaaacacg ttgtcacgtc aaataagggc ccaatcgcac tcgcataccc ggaactgcga
     3961 gcgcttgccg aggagaatgg cgtcagcctc aggtacgagg caacggtctg cggtgcgatc
     4021 ccgctcatac atgccatgca ggaagggctt gccggcaaca ccatctcccg gctctacgga
     4081 gtatttaacg gcacctgcaa ctacatcctt acccggatgg ctgcagaagg cctcacctac
     4141 gcacaggctc tggccgaagc ccgtgaactc gggtatgcag aggcagaccc cacatacgat
     4201 gttgaaggga tcgacgcggc gatcaaactg gtaatcctgg caaacaccgt cctcgatatg
     4261 cagaccacac ttgatagagt ggataggacg gggatatctc ttctgacgcc cgaggccctc
     4321 cacctggcag aagagcagga ctgtaccatc cggctgatag gtgagatcgt cccggcggct
     4381 ggagcgcttc gtgtatcgcc acgtataatc accaagacgc acccgctcgt cgtcgagggg
     4441 acactcaacg ccgttacgct ggagacggat ctcgccggtg acctcacgtt tatcggcaag
     4501 ggcgcgggct ccatcgagac cgccagtgct gtgatcggcg atatactata catccgtgac
     4561 aggtatgtcc agggttcttg agcggagaaa gcaactgatg cggctgatgc gtcaggcaac
     4621 gctggataac ggttacttca cggtcgccgg tatcgctgaa gcaaccggca tcccccggag
     4681 caccatccag gactgggtca accgtctcat cgaggagggc tgcgtcgctc tgctggaaga
     4741 gcagcggggt cggcacgctg cgcggtatgt ggcgagcagc gtgatgccgg agagcgcttg
     4801 ccgccgggta ttcaccacga ttgatggtga ggaggtcgag atctatcacg agtgcatgag
     4861 cggtgggtgc gctgcgttct gcgaattcca tcacgcccgc gcgggtggtg ctctccaatc
     4921 tgtctggcga gacgggacgc tgctccgtga gcgggcgcac ctcggacggc aggaggtcgc
     4981 tgtgggtctt gaccctgcgc cggcagtggg gattatcggc gtcttccacg aagatggccg
     5041 tatccgtcag cagatcaggt gtatcggcgg cccggcttac tctctcaccg atatgatgtc
     5101 ctttgcagag ggtgtatgtg gcgtaaccgt ccaccgcgag gggcctctgg tcgaggggga
     5161 ggtggtcacc cgggcgcttg cttacgtcgc tatcggtatc gatgacaccg atactgccgc
     5221 ggagggcgca accttcgccc tggccctcgc cctccttcag catctcacga aactcgatgg
     5281 cgtcatgccg atcggtcacc gcgtcgcgat gcttaacccc cacctcgagc ccaggactgc
     5341 cggaaactcc tgcagttgca tcgaggttgc ggtggaaccc tcaatgatcc ctcggataga
     5401 ggaggccgcg gtgcggtttg tcgccgggga ggcggcgtcc ccggagtggg gaatcgctct
     5461 ccgggagggg tttgtggtac ctcgtgacct ccgcgcttac ggtaagggcg caagagaggc
     5521 ggtgatcgag cgggaggagg ccgaggcaac tgctgaacgg tttggggcgc accttcatgg
     5581 tggccggggc gtcatcgggg cgctcgcggc ggtctcgctc atcggtctcc cgcacgaggt
     5641 gctgctcgat ccgggaatgg acgtctgcac agaccgggat ccagagcacc agtgagtctc
     5701 aatttcgccc atgacggcat agttcgatcc ccgatcctcg cgttttatct ctcccttgcc
     5761 tgagggatct cagacccggt tctcaagtaa cctataaata gtgggatatg gatgatacac
     5821 atcatcgagg cgaggagacg tgagggtcga acttgaggag gagatcgaga aactctcatc
     5881 cctggcgcga gagcgggggg cggagccgcg gcggatagcg gcccacgaga tcgtggtcgc
     5941 agagtgggtg cggttcaagt gccgctacgg ttgcaagggc tacgggaagc atatgtcctg
     6001 cccaccatac gctcccgcac ccgccgagac gcggcagatg atcggtgagt acagcaccgg
     6061 actgctcctc aagtttgagg gtataccggg gcatcccgac ctcaaacctg aagagatacc
     6121 gcttgacttt cacccgttcc tgcgcgacct gatcctgtgg gtgaactcaa cggtgcattt
     6181 cctggagaag acggctttct acgatgattt cccgaaggcc ttcgggttcg gaggataccc
     6241 ctgcatctac tgcgagcacc tccactgtgt cgcagaggaa ggggagggtg ttgttgacga
     6301 gagcatccgc aggctctgcc ggcacatgga cctcgtccgg ccgagcatgg aggctgccgg
     6361 gatagacgtc ttttccaccg ccagaagggc aggctgggat atccatatag tcccgtgcag
     6421 gaaccttgaa tacggcaaga tcgagcacgg caacatcaga tccgtggggc tcgtcctcat
     6481 cgagtgaggc gcctggaatc cctttttttt gcgctcatcc ccgggcgtgc gatttctcca
     6541 tcgatgcagt gccggcggag gagatttttg ccagttgtta tcgctgaaac gtccatggga
     6601 atcccgtttc cagcactttc acggcctcta cgtgaacacc atcacgcact gcccgccacg
     6661 ccgggggaag atggggtggt cgatgaagga gagacgttct ccagcgtttc aggaatcccg
     6721 gatcccctgg ataaactctt cagtcatatg gtaggcgtcg tcgtccctga actgcaccgg
     6781 agggtgcttc atgaagtagg ctgaaggaga gatcagaggc ccgccgatac cccggtcaag
     6841 ggcaagcctg cagcagcgga tggcgtcgat gactatcccg cctgagtttg gggagtcctc
     6901 aacggagagg cggaggtcga ggtacatggg cacgtcaccg aagatcctcc cctccatcct
     6961 gaggaaacag accttgttgt ccttctgcca gcagacgtaa tcgctcgggc cgatatggat
     7021 gttgtcagca tcaaggggct ctttcaggac tgactgaacc gcctcggtct tagatctcct
     7081 ttttgaggca aggcggtcgc ggttgagcat attcagaaaa tcggtgttgc cgccggtgtt
     7141 cagttggtat gtccgttcaa gcacaacacc ccgctggcgg aagagatcgg caagaacccg
     7201 gtgcgtgatc gtcgcgccga tctggctctt gatatcatcg ccgatgattg gaagtccgcg
     7261 ctcctggaac ctctctgccc aggcaggatc gctcgcgata aagaccggca tgttattgat
     7321 aaaccctaca cctgcctcca gcgcacactc ggcgtagaac cttgcggcct tctccgagcc
     7381 tacaggaagg tagttgagca gtatctcggc acccgagtcc tccagttccc ggactatatc
     7441 ctcacgtgac gcctcctcct cgtcagcgag aacaaacctg caatcttcct tgtagtcgag
     7501 catgtgctcc ggaaagccat ccagtacccg acccatccgg acggtcaccc ctgttcttgg
     7561 cacatccgga cagaaaacca tggtgcagtt tgggggggag aagatggcct ctgagacatc
     7621 ttttccaacc ttccttgcat cgatatcaaa cgcggcggcc acttcaatat caaaaggtcg
     7681 gtagccgccg agatcccagt gcatcagtcc ggttgtgtcc ttctcgttct ttccccggta
     7741 gtattcaatc ccctgaagca gggaactggc acagtttccg acacccacga ttgcgatcct
     7801 gatcgagtcc acaacagccc tccatcagct ggtatgtttt ctggcacaga tctttttctg
     7861 ctacctgcgc cagacacagc agaggttgca aagacacgcc tcttttcaac acctctgatt
     7921 tatgagtaat ttgcttcagc gatgaatata agggtatggt ctttgggcat gatgatggtt
     7981 ttgtggcctg agaaaggtga gtcgtctgaa cgtccagggc cgcaccgtgt ggaaacaccc
     8041 cccatcatcg cccctctgtc tctgattgcc ccctccacaa ccttttttgc tctactggtc
     8101 cagatcgaca cgaccatatt ctggaggtag taaaccatgg ttgattttga acacgcattg
     8161 attggagaag cgcttgttgg cgaaggcccg gaggttgctc atatcgatct tgtcataggc
     8221 aagaaaggga gcagcgttga gcaggcgttt gtcacggcgc tcgcatcgcc tgtaaagggg
     8281 cacaccccgc ttcttgcggt gctcacaccg aacgttccgg caaaaccatc gacgctgatg
     8341 gtcaacaagg taaccatcaa gaacgcagct caggctctcc tgatcttcgg gcctgcgcag
     8401 gcagccgtcg cgaaggcggt agtggacagc gttgctgaag ggatcatccc ggaggcagag
     8461 gccgataacc tgttgatcat agcatcggtc ttcatagagt gggatgcaaa ggacaagaag
     8521 aagatctacg actggaacta cgaggcgacg aagaccgcaa ttcaccgggc gataagaggc
     8581 gagccgaaga tgaaggacgt gctcgcacag aaggataccg caaagcaccc atttgcgtga
     8641 taaaggtccc gggacgtttt cc
//
LOCUS       DAOSZX010000061         8549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7063_length_8549_cov_19.396162, whole genome shotgun sequence.
ACCESSION   DAOSZX010000061 DAOSZX010000000
VERSION     DAOSZX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_8549_cov_19.396162"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="gatD"
                     /locus_tag="PK196_05905"
     CDS             <1..1035
                     /gene="gatD"
                     /locus_tag="PK196_05905"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HON40987.1"
                     /translation="QNPDLPELAILSTGGTIASRVDYRTGAVMSQFSAADILRAIPEL
                     GGIARYRDRQIASILSEDMQPALWQNLARAIYDEIRAGVEGVIVTHGTDTMAYSAAAV
                     RFMLRTPVPIVFVGAQRSADRPSSDNAMNALCSAAAATSDLGEVAVVMHATTNDDRCA
                     IHRATRVRKMHTSRRDAFQSIGIEPLGCVDYPSLSVSLSDEAVRRGAQEPELQDSLEE
                     RCALLQFYPGMPASVLEAFEGYAGLVISGTGLGHVSSGLVPALREMIEDGTTVVMTSQ
                     CLNGRVCDRVYNTGRDLLAAGVIEGEDMLPEAALVKLMWVLGNESDPERVRRLVQTDL
                     AGEMQRRSIP"
     gene            1032..>2023
                     /gene="gatE"
                     /locus_tag="PK196_05910"
     CDS             1032..>2023
                     /gene="gatE"
                     /locus_tag="PK196_05910"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HON40988.1"
                     /translation="MRDYDALGLKAGIEIHQQLDTAEKLFCHCPTVLRDVSERTGEFQ
                     RYLRATGSELGEIDRAAREEMRLVRKFCYYTYDTVCLVEHDEEPPTPMNPEALEIALT
                     IAKMLGMTPVEHVHTMRKVVIDGSNTSGFQRTALIALSGALPGGCRVETICLEEEAAQ
                     RVEGDTFSLDRLGIPLVEITTAPCMHNPEEVARVAAYIGMVLRSTGRVKRGLGTIRQD
                     INVSIANGARVEIKGVQDLDLIAEVVRREVERQINLLEIRDALRERGARVDQEIVDVT
                     DLFSRTNSSILKKARSILALRLCGFAGLVGREIQPDRRLGSEFSDYAKKCGVGG"
     assembly_gap    2024..2123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2124..2641
                     /locus_tag="PK196_05915"
     CDS             <2124..2641
                     /locus_tag="PK196_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HON40989.1"
                     /translation="EFGLSEDLARQVAFSERLPLFETAVAAGVRPALAARTLLATCRE
                     LARDGVEIDRVTGDEMLALLSAVEAGRAAKEAIPEILTELALTPQESGVTPEERVDAA
                     IRRVAPAVSEADVEAVVRRVVAEREGFARERGMAALGPLMGVVMKELRGSVDGKVVSE
                     ALRRELERLIS"
     gene            2697..3005
                     /locus_tag="PK196_05920"
     CDS             2697..3005
                     /locus_tag="PK196_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HON40990.1"
                     /translation="MGKTGSVQWAQVKGVKGQIRLVPASEATVKKPGPNQRFKSAAVI
                     QKRASREGQDPRRGGRGGRGGRGGRGSRGVGGPTADPRVRRAIRRSKASMLGTKQKSR
                     "
     gene            3057..3413
                     /locus_tag="PK196_05925"
     CDS             3057..3413
                     /locus_tag="PK196_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40991.1"
                     /translation="MDLKTAIRTYQFAERAKSELIIGSHLTTALAEFPIQEKPGGKRM
                     LLMLLEAVRTELEFAQRDTGHKEFKKAIDHLNEAISLVESMDLGAAAERVGRAISAAT
                     TAAQEAWDTLKGHGLL"
     gene            3400..3927
                     /locus_tag="PK196_05930"
     CDS             3400..3927
                     /locus_tag="PK196_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON40992.1"
                     /translation="MDSSEFLRFLRTRSSVRDYYGQPLEAEDIDYILACASTAPSAGN
                     REAWDVVVVTDDDIRLELSSAALDQVHVREAPAVFVVCANYVRSISRYGERGILYAVQ
                     DATIACTYMMLAAHARGLHSCWTGAFDEGEVREILDLPQHIRPIALLTVGKGRPPLEP
                     MERMPVEEHLHRETW"
     gene            3954..4292
                     /locus_tag="PK196_05935"
     CDS             3954..4292
                     /locus_tag="PK196_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HON40993.1"
                     /translation="MPDYLVTLESAWIIKDVRSMEDAVSIAISEAGKRLNPSAKFVEI
                     EAGVTECPFCEGELNTALVVAGTALVGLVLDMKVFRAESEEHAARIAKSVVGKALHDV
                     PLQVVEVQEI"
     gene            4289..5179
                     /locus_tag="PK196_05940"
     CDS             4289..5179
                     /locus_tag="PK196_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HON40994.1"
                     /translation="MIAVVGHTAIDHLFRVPKLPGRHNSTYITEHSVYFGGGAANIAA
                     GIATLGERCRLVSLVGDDFPGSDYDLWLQRLGVEQDVAVVKGARTATAYVFTDDAGDQ
                     ETFFEWGASAAFSGLEAPALDFVHMATADPDFNVRVAQKSRFASFDPGQDLLRYTPEQ
                     LGIILADIDILFSNNHEMDRMCGMLGLEREDLIASVPMTVITRGAEGSILCMDGEEHH
                     VPAVRVEAVDPTGAGDGYRAGFLTAFRRGCDPLDCCRVGAVVASFVVERPGTQTNLPD
                     RDRMLARYRKVFGEPGEIFV"
     gene            5266..7005
                     /locus_tag="PK196_05945"
     CDS             5266..7005
                     /locus_tag="PK196_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HON40995.1"
                     /translation="MDSSQYVRFEGLTRYLFSAPSWPRSLAITVVLGLLIDGAAYRAG
                     SGFFPVGTMGFTIPALVAFLLTPPLVRVSGRQITWNRSALLALACTVLGVIVSLSPTL
                     VAGPSLFPTLYAIALGLTFCIRLLVLVAVADYRQSRMALPAFTQGAAGIAVGAWLFTP
                     GFVSYALLLQVVFGLVAILLIWLIERPLKRGFHVSGLNFLNTFIAHLTDGSKRMEDFF
                     REIGEEVYVPQVSLFFSRDVGEDALFTVPNVHPGPMGDVGGGNLPRNLHDSFPEETLV
                     AHGCATHDFNLVSESEIEKIVRAVEESRKGVVFSDTASRPIRVASGSVSILCQRFGDA
                     LLMVSTRSPERTEDLDCAIGMIIMAEGRCGFSEVAFVDAHNCMTGVGSPVLPATRIAT
                     EYLDAAREGFRAARDLPLEPLSVGVSHIRVPFTREQGFGSLGVQALVTEVAGTRAAYV
                     LIDGNNVAQGVRERLRGVALLHVDEAEIMTTDTHTVNTVSGKNPVGYAVPADEIVPYI
                     EQAVREAVSDLAPARVGAATAWCEGITVFGSQRVSQLASTVNTMLSYIAPLSFMILVL
                     AFLLSVLAYIVLQ"
     assembly_gap    7068..7167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7217..8395
                     /locus_tag="PK196_05950"
     CDS             7217..8395
                     /locus_tag="PK196_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HON40996.1"
                     /translation="MNYRFAGRMKRVPESFLGELFRVSAVPGVISFAGGLPASDCIDV
                     DGIAEAARQVFAEEGRTALQYSTTDGYLPLREFIADRYRRRLGLPAAADEIQIVNGSQ
                     QSLDLAAKIFIDPGDAVGMERPGYLGAIEAFSFYEPVFHAVPMEEDGPDLAVFESVIR
                     DNAPRLFYGIPNSQNPSGTTYSDRKRRAVAEILEGSGTVFYEDDAFGELFFDGRPRMP
                     VKRYIPEQTLISGSFSKIIAPGMRIGWIYAPAPILREFNLAKQAADLHSNFLCQVILN
                     RYLRTHDLDGHVRRVAAVYGEHCRLMCDLLDDLMPAGMTHTRPEGGMFLTAALPRGVS
                     SMEVFHEGVREGVAVLPGVPFYVDGGGKETIRLNFSAVGAEEIVEGMHRLARVMHRLV
                     "
     gene            8462..8546
                     /locus_tag="PK196_05955"
     tRNA            8462..8546
                     /locus_tag="PK196_05955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8498..8500,aa:Leu,seq:taa)
BASE COUNT         1482 a         2477 c         2774 g         1616 t
ORIGIN      
        1 cagaaccccg atctcccgga acttgccatc ctctccaccg gcgggacgat cgcaagcagg
       61 gtggactacc ggactggcgc ggtgatgagc cagttctcag cagccgatat actgcgcgct
      121 atccccgaac tcggcgggat cgcccgctac cgcgaccgcc agatcgcaag catactctcc
      181 gaggatatgc agccggcgct ctggcagaac cttgcccgcg cgatctacga cgagatccgg
      241 gccggagttg aaggggtgat cgtcacccac ggcaccgaca cgatggccta ctcggccgcc
      301 gccgtcaggt tcatgctcag gacgccggtg ccgatcgtct ttgtcggggc gcagcggtcg
      361 gccgaccgcc cgagctccga taacgccatg aacgccctct gcagcgccgc cgctgccaca
      421 agcgaccttg gcgaggtggc ggtggtgatg cacgcgacca caaacgatga ccgctgcgcc
      481 atccaccggg caaccagggt caggaagatg catacctccc ggcgcgacgc cttccagagc
      541 atcgggatag aaccgctcgg ctgcgtcgac tacccctcgc tctccgtcag cctctctgac
      601 gaggctgtcc ggagaggggc gcaggagccc gaactccagg acagtctcga ggagcgatgc
      661 gccctcctcc agttctaccc cggtatgccg gcatccgtcc tcgaggcctt cgagggttac
      721 gccggtcttg tcatctccgg gacggggctc ggccacgtct caagcggcct cgtcccggca
      781 ctccgtgaga tgatagagga tgggacgacc gtcgtcatga catcccagtg tctcaacggc
      841 cgcgtatgcg accgtgtcta caacaccggc agagacctcc tcgcggccgg ggtcatagag
      901 ggtgaggaca tgctccctga ggctgcgctt gtgaaactga tgtgggtgct cgggaacgag
      961 tccgaccccg aacgcgtccg gaggctggtt cagaccgacc ttgccgggga gatgcagcgg
     1021 aggtcgatcc catgagggat tacgacgccc tgggcctcaa ggccgggatc gagatccacc
     1081 agcagctcga caccgccgag aaactcttct gccactgtcc gacggttctc agggacgtct
     1141 ctgagcggac gggggagttc cagcgctacc tccgggcaac cgggagcgaa cttggcgaga
     1201 tcgaccgtgc ggcgcgtgag gagatgcggc tcgtccggaa gttctgctac tacacatacg
     1261 atacggtctg cctggtggag cacgacgaag agccccccac cccgatgaac cccgaagcgc
     1321 tcgagatcgc cctcacgatc gcgaagatgc tcggcatgac cccggtcgag catgttcata
     1381 ccatgagaaa ggtcgtcatc gacggttcga acaccagcgg gttccagcga acggcgctaa
     1441 ttgcgctctc cggcgcgctt cccgggggtt gcagggtcga gacgatatgc ctggaggagg
     1501 aggcagcgca gcgggtggag ggggatacct tctcactcga ccgtctgggg atcccgctcg
     1561 ttgagatcac caccgccccc tgcatgcaca accccgagga ggttgcgaga gtcgcggcct
     1621 acatcgggat ggttcttcgc tctacagggc gggtgaagcg cggcctcgga acgataaggc
     1681 aggacatcaa cgtctcaatt gcgaatggcg cccgggtgga gatcaagggc gtccaggacc
     1741 tcgatctcat cgccgaggtg gtgcggcgag aggtcgagcg gcagataaac ctccttgaga
     1801 tccgcgacgc cctgcgtgag cggggcgccc gggttgacca ggagatcgtc gatgtgacag
     1861 acctcttctc ccgcacgaac tcctccatcc tgaagaaggc aagatccatc cttgccctcc
     1921 ggctctgcgg gtttgcgggg cttgtcggcc gggagatcca gccagaccgg cggcttggaa
     1981 gcgagttctc cgactacgca aagaagtgcg gggtgggcgg gatnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnngcgagtt cgggctctcc gaggatctgg cacgccaggt
     2161 ggcgttctcc gagcgcctcc cgctctttga gacggccgtc gccgccggtg tccggcctgc
     2221 ccttgcggcg cggacgctcc ttgccacatg cagggaactt gcacgcgacg gcgtcgagat
     2281 cgatcgcgtc accggggacg agatgcttgc gctgctatcg gcggtggagg ctggccgggc
     2341 cgcaaaggag gcgatccctg agatcctcac agaacttgca cttacaccgc aggagagcgg
     2401 ggtgacgccg gaagagcggg tggacgcagc catacggagg gttgcgcccg cggtctcaga
     2461 ggccgatgtt gaggcggtcg tccgcagggt tgtggccgaa cgcgaggggt tcgcccgcga
     2521 gaggggtatg gccgcgctcg gaccccttat gggtgtggtc atgaaagaac tgcgtggcag
     2581 cgtcgatggg aaggtggtca gcgaggccct ccgacgggag ctggagcggt tgatatcctg
     2641 atcctgcggg ttacttttat cagcggatca ggtcataaat ataaaggagt tttatcatgg
     2701 gaaagacagg aagcgttcag tgggcccagg tcaagggtgt gaaggggcag atccggctcg
     2761 tccctgcaag tgaagccacc gtcaagaagc ccggcccgaa ccagcggttc aagtcggcag
     2821 cggttatcca gaagcgggcg agcagggaag ggcaggaccc gaggcgaggc ggccgtggcg
     2881 gccgtggggg ccgcggcggc cgcggcagca ggggtgtggg gggtcctacg gcagatccgc
     2941 gggtccgccg ggcaatccgc cgctcgaagg cctcgatgct tggaaccaaa cagaaatcaa
     3001 gataaccctg ccttctcctt tcaatacttt ttataactgc gagtgtcagt attggtatgg
     3061 atcttaaaac cgccatcagg acgtaccagt ttgccgagcg ggcaaaatcc gagttgatca
     3121 tcgggtcgca cctgacgaca gccctggcag agttcccgat ccaggagaaa cctggcggaa
     3181 aaaggatgct tctgatgctg cttgaggctg tccgcacaga actcgagttc gcgcagcggg
     3241 acaccgggca taaggagttt aagaaggcta tcgatcacct caacgaagcg atcagtctgg
     3301 ttgagagtat ggacctgggc gccgctgcgg agcgggtcgg cagggcgatc agcgccgcaa
     3361 cgaccgcggc ccaggaagca tgggacacgc tcaaagggca tggactcctc tgagtttctc
     3421 cgttttctga ggacgcgctc ttcggtgcgg gattactacg ggcagccgct cgaggcggag
     3481 gatattgact acatcctggc ctgcgcaagc accgcgccga gcgcgggcaa ccgtgaggcc
     3541 tgggacgttg tcgttgtcac agacgacgat ataaggctgg aactttcatc ggcggccctc
     3601 gatcaggttc atgtccggga ggcccccgcg gtgtttgtgg tctgcgcaaa ctatgtccgc
     3661 tcgatctcgc gctacgggga acggggtatc ctctacgccg tccaggacgc aacgatcgcc
     3721 tgcacctaca tgatgcttgc cgcccacgcg cggggccttc attcctgctg gacgggggcg
     3781 tttgatgagg gcgaggtgcg cgagatcctc gatctcccgc agcacatccg tccaatcgcc
     3841 ctcctcaccg ttggaaaggg gagaccgccg cttgaaccga tggagcggat gccggtagag
     3901 gagcatctgc accgcgagac ctggtagata aatactgcat tttgggagag agtatgcctg
     3961 attatttggt tacgcttgaa tctgcctgga taattaagga tgtcaggtcg atggaggatg
     4021 ccgtgagcat cgcgatcagc gaggcgggaa agcggctcaa cccctcggcc aagttcgtgg
     4081 agatcgaggc cggggtgacc gagtgcccct tctgcgaggg tgagttgaac acagcgcttg
     4141 tggttgccgg taccgcgctt gtcgggcttg tgctggatat gaaggtcttc cgtgctgagt
     4201 cagaagagca tgcggccagg atagcaaaat cggtcgtcgg gaaggcgctc cacgatgtgc
     4261 cgctccaggt tgtggaagtg caggagatat gatcgccgtt gtcgggcata ccgcaatcga
     4321 tcacctcttc cgggtgccta aactcccggg acggcataac tcaacctaca taaccgaaca
     4381 ctcggtctac ttcggcggcg gggcggcaaa catagcggca gggattgcga cgctcgggga
     4441 gcgctgccgg ctggtctccc tcgtgggtga cgactttccg ggcagcgatt acgacctctg
     4501 gctgcagcgc ctcggtgtcg agcaggatgt tgccgtggta aagggcgcca ggacggcgac
     4561 cgcctacgtc ttcaccgacg atgccggtga ccaggagacc ttctttgaat ggggcgcgtc
     4621 agccgcgttc agcggcctgg aggctcccgc tctcgatttt gtccacatgg caaccgctga
     4681 cccggacttt aacgttcggg tggcgcagaa gagcaggttt gcctctttcg acccggggca
     4741 ggatctactc cgctacaccc cggaacagct tgggatcata cttgcagaca tagatatcct
     4801 gttctcgaac aaccacgaga tggaccggat gtgcggtatg ctggggctgg aacgcgaaga
     4861 tcttattgcg tctgtcccga tgacggtcat aacccgtggg gctgagggaa gcatcctctg
     4921 catggacggc gaggagcatc acgtcccggc cgtccgtgtg gaggctgttg acccgaccgg
     4981 tgccggtgac ggttaccggg caggtttcct cacggcgttc aggaggggct gcgacccgct
     5041 cgactgctgc cgtgttggcg cggtggttgc gtcgtttgtc gttgagcggc cgggcaccca
     5101 gacaaacctt ccggaccgcg atcggatgct tgcacgctac cggaaggtct ttggcgaacc
     5161 tggagagatt tttgtctgag atatggggaa cgctctgcag attgccgaga caaggctgtc
     5221 tgcttttaac tctgctccgg gagcggcacc cggtggtgca tcctgatgga ctccagccag
     5281 tacgtgcggt ttgaggggct cacccgctac ctcttctcag ctccttcctg gccgcggtcg
     5341 cttgccatca ccgtggtgct cgggctcctc atcgacgggg cggcctaccg ggctggcagc
     5401 gggttctttc ctgtcggcac catgggtttc acgattccgg cgctggttgc attcctgctg
     5461 acgccgccgc tggtgcgggt ctccggcagg cagatcacct ggaaccggtc ggctcttctg
     5521 gcacttgcct gcacggttct tggggtgatc gtgagcctct caccgacgct ggttgccggc
     5581 ccctcgctct ttcccacgct gtatgcgata gcgctagggc tgaccttttg catccggctg
     5641 ctcgtccttg ttgccgtggc tgattaccgg cagagccgga tggctcttcc cgcgttcaca
     5701 cagggtgctg ccggcatagc ggtgggtgca tggctattta caccggggtt tgtttcctac
     5761 gccctgctgc tccaggtggt cttcgggctg gttgccatcc ttctgatctg gttgatcgag
     5821 cggccgttga aacgggggtt ccatgtcagc gggttaaact tcctcaacac cttcattgcg
     5881 cacctcaccg acggctcaaa gaggatggag gacttcttcc gcgagatcgg cgaggaggtc
     5941 tacgtcccgc aggtctcgct cttcttctcc cgcgatgttg gagaggatgc cctctttacc
     6001 gtcccgaacg tccaccccgg gccgatgggc gatgtcggcg gcggaaacct cccccggaac
     6061 ctgcatgact cgtttccgga ggagacgctg gttgctcacg gctgcgccac ccatgatttc
     6121 aatctggtct cggagagtga gatagaaaag atcgtccgcg ctgtggagga gtcgcggaag
     6181 ggggttgtat tctccgatac agcgagccgg ccaatcaggg ttgcctcggg ctcggtctcg
     6241 atactctgcc agaggtttgg ggacgctctc ctgatggtga gcacaaggtc gccggagcga
     6301 acggaggacc ttgactgtgc gatagggatg attatcatgg ccgagggccg ctgcgggttt
     6361 tcggaggttg cgttcgtcga cgcccacaac tgtatgaccg gcgtggggtc accggtactc
     6421 cctgcaaccc ggattgcaac ggagtacctg gatgccgcac gcgaggggtt cagggcggcg
     6481 cgggatctgc cgctggagcc tctttcagtg ggggtgtcac acatccgggt tccgtttaca
     6541 cgggagcagg ggttcgggtc gctcggggtg caggcgctgg tgacggaggt tgcggggacg
     6601 cgggcggcct acgtcctgat cgatgggaac aatgtggcac agggcgtccg ggagcgcctg
     6661 cgcggggttg cccttctgca tgtggatgag gcggagatca tgaccacgga tacgcacact
     6721 gtaaacacgg tcagcgggaa gaacccggtc ggatacgcgg tgccggcgga tgagatcgtc
     6781 ccctacatcg agcaggcggt gcgggaggcg gtctctgatc tcgcgcccgc ccgcgtgggg
     6841 gcggcaacgg cctggtgtga ggggataacg gtctttgggt cgcagcgggt ctcgcagctt
     6901 gcaagcacgg tgaacacgat gctctcgtac atcgcaccgc tgagtttcat gatacttgtc
     6961 ctggcgttcc tgctctcggt cctggcctac atcgtgctgc agtaagttgt tgctcagggg
     7021 tttttccccc tctctcttgg gcgttgtcag ccggagcgtc ttttcagnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gccggccatc gggtgggttt ttctctctgt
     7201 acgtccagga attgtcatga actatcggtt cgccggccgg atgaaacgcg tcccggagtc
     7261 gttcctcggg gaactcttcc gggtctcggc tgttcccggc gtgatatcgt ttgccggagg
     7321 cctcccggcc tcggactgca tcgatgtcga tgggatcgcg gaggctgccc gtcaggtctt
     7381 tgccgaagag gggcggacgg cgcttcagta cagcacgacg gacggttacc tccccctccg
     7441 ggagttcatc gccgaccgct accggagaag gctcggcctc ccggcagccg cggacgagat
     7501 ccagatcgtg aacggttcgc agcagtctct tgacctggcc gcaaagatct tcatcgaccc
     7561 cggggatgcc gtcgggatgg agcgccccgg ctacctcggc gcgatcgagg ccttctcgtt
     7621 ctacgagccg gtctttcacg cggttcccat ggaggaggac gggccggacc tcgctgtgtt
     7681 cgaatcggtc atccgcgaca acgccccgag gctcttctac ggcatcccca actcgcagaa
     7741 cccttccggg acgacgtact ctgaccggaa acgccgggct gtcgcggaga tccttgaggg
     7801 gagtgggact gtcttctacg aggacgacgc cttcggggaa ctcttcttcg acggcaggcc
     7861 gcggatgccg gtgaaacgct acatcccgga gcagacgctt atctcgggct cgttctcaaa
     7921 gatcatcgcc ccggggatgc ggataggctg gatatacgcc ccggcaccca tccttcgcga
     7981 gttcaacctg gcaaaacagg ctgccgatct ccactcaaac ttcctctgcc aggtgatact
     8041 caaccgttac ctgagaaccc acgacctgga tggccacgtc cggagggttg cagcggtcta
     8101 cggcgagcac tgccggctga tgtgcgacct cctcgacgac ctgatgccgg cggggatgac
     8161 gcacaccaga ccggagggcg ggatgttcct gaccgccgcc ctgcctcgtg gcgtctcctc
     8221 catggaggtc ttccacgagg gggtgcggga gggtgttgcg gttctgccgg gggtgccgtt
     8281 ctacgtcgac ggcggcggga aagagacgat acggctgaac ttctcggcgg tgggagcgga
     8341 agagatcgtt gaggggatgc accggctggc acgggtgatg caccggctgg tgtgagggtg
     8401 gcgaccggcg gcgggcttaa tacatctcct cttcaaatac catatggacg attttgggaa
     8461 agcgagggta ggcaagcctg gccaaaaccg gcggacttaa gatccgttct cgcaggagtt
     8521 cgtgggttcg aatcccaccc ctcgcatct
//
LOCUS       DAOSZX010000062         8446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7164_length_8446_cov_17.938863, whole genome shotgun sequence.
ACCESSION   DAOSZX010000062 DAOSZX010000000
VERSION     DAOSZX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_8446_cov_17.938863"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..837)
                     /locus_tag="PK196_05960"
     CDS             complement(1..837)
                     /locus_tag="PK196_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON40997.1"
                     /translation="MALKSVTDAVALLGRHPILWLIGLVMGVLAVLDLVVPLEVGTFY
                     AQPLLLLQVLVMPFVAGGVYGVIKEKNFSGSEFVRSGKHYYFRILLPSFVIYFAIMLT
                     VFLLAIPLALIGGGIAANLAALILGVALSIAFFTFFYDTAAVFEGANVFESIRRSVEF
                     VMNNLWSVLAFYLINILVLVVLGFLALFAWSALLVDKLEPLVSMSPAELQTLMPEDLL
                     ALIGTEGVWITAIVYAVVIFIFSVFHYTYKASFFSNRASGAARLQELQGEYDEKGRWY
                     KY"
     gene            complement(840..1430)
                     /locus_tag="PK196_05965"
     CDS             complement(840..1430)
                     /locus_tag="PK196_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HON40998.1"
                     /translation="MGFVTRNTYYFDSPGAENTHDAAHFAVERAQELGIKKIVVASTT
                     GRTALLFRDAMKGTGLELIVVTHVVGFSKPGEWEFSPEAAAALRADGVKIVTGTHALS
                     GLERAISRSQRLGGSSRTEAIAEALRRIVAVGLKVAVECVLIAADQGVVGVDEEVVAA
                     GGTASGADTVCVIRPAHTSSFFDLQVREIVAMPRNR"
     gene            1544..2713
                     /locus_tag="PK196_05970"
     CDS             1544..2713
                     /locus_tag="PK196_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HON40999.1"
                     /translation="MITLAIAGKPNCGKSSFFKAATLAQAEIASYPFTTIDANHGVAY
                     VRTVCPCKEMHVPCGNCVDGIRFVPVGLIDVAGLVPDAHLGRGLGNQFLDHLRQADAI
                     LHIVDASGATDAEGNPVEIGSRDPIKDIEFLQYEISMWMYGILSRNWAKLQRQAQARE
                     FSLATAIAEVFAGLGITYEHVHDATKTVGVDLRTAGEEEMVEFCRELMRISKPMLIVG
                     NKADQAPKKCLDRLAGRDLIFASAAGELALRMAAEGKFIRYLPGDRSFEILPGANLSA
                     AQRAALQKIAVFMEKFDGTGVQKALDTAVFKLLDRIVVFPVEDEHKLTDSKGRVLPDA
                     FLMKRGSTPRDLAYQVHSDIGEGFLYAIDARTGMRIKDTYELKNGDIIKIVSVRR"
     gene            2805..3125
                     /locus_tag="PK196_05975"
     CDS             2805..3125
                     /locus_tag="PK196_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41000.1"
                     /translation="MGGEVYQAQVLRNFFDTITGADRNLTRIYMCVVSLAKLRMESPE
                     KMTFLIDQLRKSRQKRELSVDIIDYMCDVARDLELTTVQTAFGVRDISRVADEFSGIS
                     LDSL"
     gene            3219..3839
                     /locus_tag="PK196_05980"
     CDS             3219..3839
                     /locus_tag="PK196_05980"
                     /inference="COORDINATES: protein motif:HMM:NF039541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HON41001.1"
                     /translation="MVRRDTARIPAAGGKRVSRDLFYSCGGGVGRTRDAPKWRCSRRR
                     SLEEPLSIFPAHRYAMNVLLIQSEGVDLYTTLLASETSRRALRFYRPDRLPYGIRVRT
                     ASLGSALALVSELRWYIQRYVQDVLFEPEDGIFYSSALARQVYERDVRPSHPWSHRCL
                     YRVAGDRLIDLIWMEEGTRPESYSDRTERGDTLLEVWCTDEESPGD"
     gene            complement(3850..4485)
                     /locus_tag="PK196_05985"
     CDS             complement(3850..4485)
                     /locus_tag="PK196_05985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON41002.1"
                     /translation="MTADPSPWDEDYRRRGSLWGGAPAPLPEIPADARVLEIGCGNGK
                     TLAALARRSLHVIAVEISREALLLARRHPATAGAEFVLGDARHLPFLDGVFDMVFLVH
                     LAGHLPARDRLTLASEVCRVLAAGGTLIFRAFSVEDMRAGKGVEVEPKTFRRGSGIIT
                     HYFTETEALELFAPLIPVSLRTDRWQMRVRGTDLPRAEVEGVFLKETERAP"
     gene            5597..6754
                     /locus_tag="PK196_05990"
     CDS             5597..6754
                     /locus_tag="PK196_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41003.1"
                     /translation="MDLTDAAAMMLALLIFAGIVLGPIMAETAWPEAPEPEEPEDLAA
                     VLTGLNERILSGKPINLTPLTMPDTAAALIAPGENETAIHGENSTPLMPEYDDDELIG
                     LVENRSVSLMLLAMQNAHALYAWDEKTVREHAAALQNLARSTLKDSAGFDVSSDLEDV
                     KTNFTLALESYAAYADIVQQNNKINETQVDLAFQELERGSRHLREALDGLDQQTLHVP
                     AEVVDVNLSVVQPSAVPADALVLMQRYVYDDRIGANEISLTLESAGIIHAYCLKGEEI
                     TAEPGRAFLVVEVKVMNLGHKGERRVYTIRTPDVSAFTLHYRGTTYSPIELVPGTTLG
                     VPYAGVTLNRYEKKHGYIVFDVPDAINLSECFIRVNLGEAGSPVWVLRKAG"
     gene            6950..8014
                     /locus_tag="PK196_05995"
     CDS             6950..8014
                     /locus_tag="PK196_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41004.1"
                     /translation="MRRSRAAMVVVACCIGAACLIFALVPSISAEVFPHLRAEGQRPD
                     SVNESDLTAAIVPPTDDERLESLIAGGSVRLLELSVGQIGALHAGNDSLLRSQAAGMV
                     SLAEQLRLDAAALWVSPENESVRSQFIAALDEFAAAGSMLENGIPTDRSVRDDALEHL
                     ARGTEVLSETLQGYTPTPTVEPEGSTLPADLAAEADPEFPGALRPGERFIYDDSSGDN
                     TASIIIGGFTLSNGFETIGAKPVEYTPGPGNVYLLVTVQATHLGYRGEGTSSRIRTPA
                     ESAFTLYYNGETYRPLQAPGPTSRGGSYSSLVLERHESRAGYLFFEVPEDFDPANAYL
                     QVRVGSNSPVWHLDPAKAAG"
BASE COUNT         1775 a         2494 c         2589 g         1588 t
ORIGIN      
        1 tcagtattta taccaccgcc ccttctcatc gtattccccc tgcaactcct gcaaccgcgc
       61 cgcaccagag gcgcggttgc tgaaaaaact cgccttgtat gtgtagtgga ataccgaaaa
      121 aatgaagatc acaacggcat agacgattgc agttatccat accccctctg tcccgatgag
      181 ggccaggaga tcctccggca tcagcgtctg aagttctgcc gggctcatgc tgacgagcgg
      241 ttcaagttta tccacgagga gggccgacca ggcaaagagg gccagaaacc cgagaacgac
      301 cagaacgagt atgtttatca ggtagaaggc caggacgctc cagaggttgt tcatcacaaa
      361 ctcaacgctc ctccggatcg actcaaagac gttcgccccc tcaaagaccg ctgcggtgtc
      421 gtagaagaac gtgaagaaag caatcgagag agccacgccg aggatgagtg cagccaggtt
      481 tgccgcgatg cccccaccga tgagcgcgag cgggatcgca agcagaaaga ccgtcagcat
      541 gatggcaaag tagatcacaa acgatggcag gagtatacgg aagtagtagt gcttccccga
      601 tcgcacaaac tccgagcctg agaagttctt ctccttgatc acaccgtaaa ccccgccggc
      661 aacgaatggc atcacgagaa cctgcaggag cagcagcggt tgcgcataga acgtcccgac
      721 ctcgagcgga acgacaagat cgaggacggc gagaacgccc atgacaagac cgatcaacca
      781 gagtatgggg tgccgcccaa gtagtgcgac agcatcggtg accgatttga gcgccatgtt
      841 caccgattcc ttggcatggc aacgatctcg cgcacctgca gatcgaagaa ggatgaggtg
      901 tgtgcgggcc gaatgacaca gactgtatca gcaccgctgg cggtgccgcc tgccgcgacc
      961 acctcttcat cgacgccgac cacaccctgg tcggccgcga taagaacgca ctcaaccgcg
     1021 accttcaggc cgaccgcgac gatgcgccgc agcgcctcgg cgatcgcctc ggtgcgggaa
     1081 gatcccccaa gccgctgtga acgtgatatg gcgcgttcaa gcccggagag agcatgcgtc
     1141 cccgtgacga tcttaacacc gtccgcacgg agagccgcgg ccgcctcagg tgagaactcc
     1201 cactcaccgg gttttgagaa accgaccaca tgggtgacaa cgatgagttc aagacccgtt
     1261 cccttcatgg catcccggaa gagaagcgct gtgcggccgg tggtgctcgc gacaacgatc
     1321 tttttgatcc cgagttcctg tgcccgctcg acggcaaaat gggctgcatc gtgcgtgttc
     1381 tccgcgccgg gtgagtcaaa gtagtatgta ttccgcgtta caaagcccat gcactcatgt
     1441 tgtcattcct cctaattgaa tctccccccg gggccggcgg gaaagaaggt gataaatggt
     1501 ggatgcccca acactactct caacgctaac aggagacagc ctcatgatca cactggccat
     1561 tgcaggaaaa ccgaactgcg ggaaatcatc gttttttaag gcggcaaccc tggctcaggc
     1621 tgagattgcc agttacccgt tcacaaccat cgatgccaac cacggcgtcg cgtatgtaag
     1681 gaccgtctgc ccctgcaagg agatgcatgt cccgtgtggg aactgtgtgg acggtatccg
     1741 atttgttcct gtcgggctga tcgatgtggc aggtcttgtc cccgatgctc acctggggag
     1801 ggggcttgga aaccagttcc tggaccatct caggcaggca gatgcgattc tgcacatagt
     1861 ggatgccagc ggtgcgacgg acgctgaagg aaacccggtc gagatcgggt cgcgggaccc
     1921 cataaaggac atcgagttcc tgcagtatga gatctcgatg tggatgtacg gcatcctctc
     1981 gcgaaactgg gcgaaactcc agcgtcaggc gcaggcacgg gaattctcac tggcaacagc
     2041 gattgcggag gtgtttgccg gtcttggtat aacctatgaa cacgttcacg atgccaccaa
     2101 aacggtcggg gtggacctgc gaacggcggg agaggaggag atggtggagt tctgccgtga
     2161 gttgatgagg atcagcaaac caatgctgat cgtcgggaac aaggccgatc aggcgccgaa
     2221 aaagtgcctt gaccggctgg cagggcgcga tctcatcttt gcaagcgcgg ccggtgaact
     2281 tgccctcagg atggccgcag aggggaagtt tatccgctat ctcccgggtg atcggagttt
     2341 tgagattctt cccggcgcaa acctcagcgc tgcccagcgt gccgccctgc agaagatagc
     2401 cgttttcatg gagaagtttg atgggacagg cgttcagaag gcgctggata cggcggtctt
     2461 caaacttctg gaccggatcg tggtcttccc ggtcgaggat gagcataaac tcaccgacag
     2521 caaggggagg gtgcttccag atgcgttcct gatgaagcgc gggtccaccc ctcgcgacct
     2581 tgcctaccag gttcactcgg atatcggtga ggggttcctc tacgctatcg acgcgaggac
     2641 cgggatgcgg atcaaggaca cctacgaact caagaacggt gatatcatca agatcgtcag
     2701 tgtccgcagg tgaacggccg ggttcggcga gcatgataca gatggttctt gccgcgcgat
     2761 caaaccgata agcatttatg caaaattgag gataaacgat tgtcatgggc ggcgaggtct
     2821 accaggccca ggttctccgg aacttctttg acaccatcac cggggcggac aggaacctga
     2881 cgaggatcta catgtgcgtg gttagccttg ccaaacttcg gatggaatcc cctgagaaga
     2941 tgacgtttct catagaccag ctccgcaagt cgagacagaa acgagagctc tctgtcgata
     3001 tcatcgatta tatgtgcgac gtggcgcgtg accttgaact caccaccgtc cagacggcgt
     3061 tcggcgtcag ggacataagc agggtggcgg atgagttcag cgggataagc ctcgactcgc
     3121 tctgatcttt ttccccctga tccggcaggt atgacacggt ctttttttga tggaggctga
     3181 gcaatggcgg cgcgggtata gccgcccgat atacatacat ggtgcggaga gataccgcca
     3241 ggattccggc ggcgggtggg aagcgggtca gcagggatct tttctattcc tgcgggggcg
     3301 gggtggggcg gacgcgggat gcccccaaat ggcggtgcag cagacgaaga tctcttgagg
     3361 aacccttatc gatcttcccc gcgcacaggt atgccatgaa tgtcctcctc atacagtccg
     3421 agggggtcga cctctatacg acgctgcttg cctccgagac cagcaggcga gcccttcgtt
     3481 tttaccgtcc tgaccgactc ccatatggta tccgcgtccg gacggcgtcg ctcgggagcg
     3541 cacttgctct tgtctccgaa ctccggtggt atatccagcg ttacgttcag gatgtcctct
     3601 ttgagcccga ggacggcatc ttctactcgt ccgcgcttgc ccggcaggta tacgagcgcg
     3661 acgtacggcc ttcacaccca tggagtcacc gctgcctcta cagggttgcc ggtgaccggt
     3721 tgatcgatct catatggatg gaggagggga caaggccgga gtcatacagc gaccgcaccg
     3781 agcgggggga tacgctgctt gaggtctggt gcaccgatga ggagtccccg ggggattagc
     3841 cccccgggtc tacggcgcac gctctgtttc tttcaggaag accccttcaa cctcggcccg
     3901 ggggaggtcg gtgcccctga cccgcatctg ccagcggtct gtccggagcg agacggggat
     3961 gagcggcgca aagagttcaa gggcctcggt ctctgtgaag tagtgggtta ttatcccgct
     4021 cccccgccgg aacgtcttcg gttctacctc cacccccttc ccggcgcgca tgtcctcgac
     4081 ggagaaagcc cggaatatga gcgtgccccc ggctgcgagc acccggcaga cctccgatgc
     4141 cagcgtgaga cggtcgcggg cgggcaggtg gccggcgaga tgaaccagga agaccatatc
     4201 aaacacccca tccaggaagg ggaggtggcg ggcatcgccc aggacaaact ctgcccccgc
     4261 cgtcgcgggg tgccggcggg cgaggaggag ggcctccctg gagatctcga ccgcaatcac
     4321 gtgcaaagag cgtcgggcga gcgccgccag ggtctttccg ttcccgcagc cgatttcaag
     4381 gactctggca tcggcgggga tctccgggag cggcgccggg gctcctcccc agaggctccc
     4441 ccgcctgcgg tagtcctcgt cccacgggga tggatcggcg gtcacggatc agaggtcgac
     4501 gctcgccggc ataacgtttc ggacgtgcag accatggcgg ggtgttcgga gacggcattg
     4561 cggagaggtt agagagacgg gggatcgacg aaggggagtg tcggggcgag atgccgaaga
     4621 cgggggcggt atctggcagt ctcgccgcgg tctcacctga caccccacaa agatccttct
     4681 tcagcccaga gcactgtatc cgggccaatc gccacgcact gcccgccccc tcggaagggg
     4741 gcggccgggc ggtgggggtg gtcaacgtaa accgttcaac cagagacaca gcaacagggg
     4801 agggtgatgt tccacccccc tccccgtcag ccccactccc agggggcgat tggccattga
     4861 cacactgccc gggaggagat cgtctcgccg cggtctcacc tgacactcct gaaagatact
     4921 gttcccgtcc caaggcacgg ccatcgagcc aatcgcccct cactgcccgc cccctcggaa
     4981 gggggcggga ggaggccgcg gagcggccgg gaggtggggg tggtcaacgt aaaccgttca
     5041 accagagaca cagcaacagg ggagggtgat gttccacccc cctccccgtc agccccaccc
     5101 ccagggggcg attccccatc gacacatttt ccgggggaga tcctctctct gccgtcgatg
     5161 tgaacaccct gccgaaatcc tgtctcgtcc ccgtgcactg tatccgggcc aatcgccacg
     5221 cactgcccgc cccctcgaaa gggggcggga ggaggccgcg gagcggccgg gaggtggggg
     5281 tgatcgactg ctcgcatgga aagacgtctt cggtgacacg cgagcgacag gggaggggga
     5341 tgttccaccc cctcacccgg ttcccggtga aaaacccccc gaagaatcct gtctcgtccc
     5401 cgtgcactgt atccgggcct ataaccacag actgcccgcc cccactgtga aggggtggtc
     5461 tgcctcacgc gggaaagagt gtccaccgcg gcggtgattc gagtgatcaa accatgaatt
     5521 gacctggttt gatgggcccg cgctgagaca gggaaacccg gtaaatcttt ttaactcaga
     5581 cgatcgacct accagcgtgg acctgacgga tgccgccgcg atgatgctcg ccttattgat
     5641 atttgcgggt atcgttctcg ggcccatcat ggcagagaca gcctggcccg aagcccccga
     5701 accggaggaa ccggaggatc tcgccgccgt cttaaccggc ctcaacgaga ggatcctcag
     5761 cggtaaaccc atcaacctga cccctctaac catgccggac acggcagccg cgctgatagc
     5821 ccctggcgag aacgagacag ccatccatgg tgagaacagc acgccactca tgcccgaata
     5881 cgatgacgac gaactgatcg gtctggtcga gaaccgcagc gtctcgctca tgctgcttgc
     5941 catgcagaac gctcacgccc tctacgcctg ggatgaaaaa acggtcaggg agcacgccgc
     6001 agcactccag aatctcgcga ggagcaccct caaagattca gccggttttg acgtctcaag
     6061 cgacctggaa gacgtgaaga ccaatttcac ccttgccctt gagtcttatg cggcatatgc
     6121 cgatatcgtg cagcaaaaca acaaaattaa cgaaacgcag gtggacctgg cgtttcagga
     6181 acttgaacga ggttcacggc atctgcgcga ggctcttgat ggccttgacc agcagaccct
     6241 gcacgtgccg gcagaggttg tggatgtcaa tctctcggtg gtgcagccct ctgccgtccc
     6301 tgccgatgca ctggtgctga tgcagcggta tgtctacgac gaccggatcg gggcaaacga
     6361 gatatcgctc accctggagt cggcaggtat aatccatgcc tactgcctga aaggggagga
     6421 gatcacagca gaaccggggc gggcgttcct ggtggttgag gtgaaggtca tgaacctcgg
     6481 ccataaggga gagcggcgcg tatacacgat ccggacgcct gatgtcagtg cattcacgct
     6541 ccattaccgc ggaaccacct actcaccgat agaactggtg cccgggacaa cgcttggcgt
     6601 gccgtatgcc ggggtgacgc tgaatagata cgagaagaag catggctaca tcgtatttga
     6661 tgtccccgac gcgatcaacc tgagtgagtg cttcatacgg gtcaacctgg gtgaggcggg
     6721 ctcacccgtc tgggtgctga gaaaggctgg ctgaggtgcg gtatttgaaa tgacgcccct
     6781 gccgccccct ctcagcaccg ctctgccccg ccgccatatc tcaggcatgg ccttgatggt
     6841 tgagtttgca gccggcaagg ggcagtgtat gggaacggcc ggaagatggc tctctcccac
     6901 tggacacaaa gaatattcct gttgcggggc ataggttttt ccagtggcta tgagacgctc
     6961 gcgcgcggca atggtcgttg tggcatgctg tattggagca gcctgtctca tatttgccct
     7021 cgtcccctcg atcagtgcgg aggtctttcc tcatctccgg gcagaggggc agcgtcccga
     7081 cagcgttaat gagagcgatc ttacagccgc aatcgtgcct ccaaccgatg acgagaggct
     7141 ggagtcgctg atcgccgggg ggagcgtcag gcttcttgaa ctctccgtcg gccagatagg
     7201 tgccctgcat gccggtaacg attcccttct ccgcagccag gctgccggga tggtctctct
     7261 tgcggagcag ctccgtctcg acgctgccgc tctttgggtc tcgcctgaaa acgagtcggt
     7321 gcgatcgcag ttcatcgccg cgctggatga gtttgcagca gccggcagca tgcttgaaaa
     7381 cggcatcccc actgaccgaa gcgtgaggga cgatgcgctg gagcatcttg cccgcgggac
     7441 agaggttctc tctgagacgc tgcagggcta cacccccact ccaaccgtgg agccggaggg
     7501 ttccacccta cctgcagacc tcgccgctga ggccgatcca gagtttcctg gcgctctgag
     7561 gcctggcgaa cggttcattt atgacgattc aagcggggac aacaccgcct cgataatcat
     7621 cggggggttt accctgtcga acggttttga gacgattgga gcaaaaccgg tggagtatac
     7681 gcctgggcca ggaaatgtct atctcctggt caccgttcag gcgacgcacc ttgggtacag
     7741 gggtgagggc acaagcagcc gtataaggac accggcggag agcgctttca ccctctacta
     7801 caacggtgag acctatcgcc cattgcaggc gcccgggccg acatcccggg gagggtcgta
     7861 ctcgagcctg gttcttgaga ggcacgagtc gagggctgga tacctcttct ttgaggtgcc
     7921 ggaagacttt gacccggcaa acgcctacct acaggtcagg gtcggcagta acagcccggt
     7981 ctggcacctt gaccctgcga aggccgcagg gtagctcccg ctcccatcga tccgcaccgt
     8041 tcgggttgaa tcatctttgc tggatcatcc ctcacgcggc cggcggtgcc gctcaacggt
     8101 gcgggcattg tgtggcgatc gccgcacgga aagccgcgct tgggggggtg aacccggaat
     8161 tcggtgagtc ttcagcgcga cccgctgaga cgatccttgc tccatggaac tgtatcgagg
     8221 tagaatcgca gaaaggtggg atccagcata ctcaacccta cattcaaata attgcgtccc
     8281 tacaccctca tctagaatgc ctgagcggag tgcggacagg atggggcagg cgcggcggga
     8341 tcttgccatg gccaggcagg ccagggatac cgggttcttt gaatgggcgt gcttcatctt
     8401 cgtataggca ggagacccca gccttcagac tggggaggaa tgccgt
//
LOCUS       DAOSZX010000063         8353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7278_length_8353_cov_20.523500, whole genome shotgun sequence.
ACCESSION   DAOSZX010000063 DAOSZX010000000
VERSION     DAOSZX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7278_length_8353_cov_20.523500"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1473
                     /locus_tag="PK196_06000"
     CDS             184..1473
                     /locus_tag="PK196_06000"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HON41005.1"
                     /translation="MAMPTLQVALDLLDLDRAVEIAAEAVQGGADWIEVGTPLIKSEG
                     MRAVRTLRERFPDHEIVADMKIADTGAIEVEMAAKSGAGIVCILADADDTVIAEAVRS
                     ARKYGVRLMADLINVSDPVARAREVAELGVDYINAHVGIDQQMIGRSSLDLLHRLTGE
                     VNAPIAVAGGLDAETASAAVAAGATIVIVGSNIIKAADVTAATRRVREAIDRPEIRPR
                     EKESPDAVIRRLFEAVSAPNVTDAMHRKGAMAGIISICGRVRAVGPAVTVRTVSGDWA
                     KPVEAIDIARPGDVLVISNDGRKDISPWGELATHSAKNRGLAGIVIDGAVRDVDDIRE
                     MGFPVFATATVPNAGEPVGLGEINTEILCCGQEVRPGDWIVADESGVVVVPRDRAYEI
                     ARRAMEVRKTEERIREEIRRGRTLSEVSELLKWEKRR"
     gene            1518..2204
                     /locus_tag="PK196_06005"
     CDS             1518..2204
                     /locus_tag="PK196_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HON41006.1"
                     /translation="MQNIREEVVGVLQAVFPIVVVIILLEFFVLQATPADLIQFLLGS
                     GMVILGIALFLTGVKTGLLPIGEAIGSELPARGSPTLLIAGSFLFGFLAAIAEPNLRV
                     LTTTLDTISGGIPSGPLLFVIAVSVGLILTAATLRIVFGFPLAYLFAAAYGAIIVLAP
                     FTAPDFLAVAVDSGAITTGLMTIPVILALGTGVSSVLTGRSPLTDGFGLVGLASIAPI
                     ISIMLLGVIY"
     gene            2228..2932
                     /locus_tag="PK196_06010"
     CDS             2228..2932
                     /locus_tag="PK196_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HON41007.1"
                     /translation="MDQVIFEAAQALLPLLIFFGVFQLLYLKLPRVYVVNLAKGILLA
                     FLGMIFFLQGVDVAFLPAGREIGEAIAAFDQRWLLIPLGFFLGFLVTYAEPEVRVLGY
                     QVERSSDGYIRESLIVYTLSFAVAVAVSLGMARLVYNIPLLGIIIPGYLLVIILLIFT
                     DRDFVGIAFDSGGVATGPMAVTFLLSLAIGVATGIEGLNPITDGFGLIALIALAPILS
                     MLLLGTLYRRTKGETT"
     gene            2929..3273
                     /locus_tag="PK196_06015"
     CDS             2929..3273
                     /locus_tag="PK196_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HON41008.1"
                     /translation="MNNDYGNELIVTIVKRGWSHTVVDAARKAGAEGATILFGRGSGI
                     NEVKTLLGITIEPEKEIILTVVGAERTDAVLDAILAAGELDRPGMGMAFVVPIRRVAG
                     RVHMFRKNGGDK"
     gene            complement(3373..3807)
                     /locus_tag="PK196_06020"
     CDS             complement(3373..3807)
                     /locus_tag="PK196_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HON41009.1"
                     /translation="MEKSSYIIKQISVFSENRPGRLAAIAAALRDARINILAFSIAEA
                     NGFGVVRALVDRPDDAYRTLTDLGFRVSFTDVIGVKMRDEPGGLSEIASVLGDAGINI
                     EYAYAYSGKDAAVLILRVDKVEDAVREILDHGGKLLKIPHSP"
     gene            complement(3807..>4073)
                     /locus_tag="PK196_06025"
     CDS             complement(3807..>4073)
                     /locus_tag="PK196_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HON41010.1"
                     /translation="TLLEIPEVGKHFQIVVDRKGALDSMLVRVEVSEEAFSDRIPDLM
                     EIKRTVEHRLRSSLNVSVEVELVEPGTLPRFEGKSRKVIDRRSL"
     assembly_gap    4074..4173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4174..4910)
                     /locus_tag="PK196_06030"
     CDS             complement(<4174..4910)
                     /locus_tag="PK196_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON41011.1"
                     /translation="MPPWNPRIEEMPIDELQRLQFKLLKSQVYRLYSFNEFYRRRMKE
                     HGVHPDDIRNLEDLAKLPFMHKSDLRDGYPDRIFTAERSELVRYHVSSGTTGKPTVVG
                     YTERDIANWSESLARAFSSCGLGRGDVLQVSYGYGLFTGGLGAHYGAERLGATVLPTS
                     VGNTERQVELMQDLHVTAIACTPSYLLHIAEVAEKMGVSIRDDTDLQMGFLGAETWSE
                     QMRTRIQDWLGIRAYDIYGTSELSGPM"
     gene            complement(4912..6204)
                     /locus_tag="PK196_06035"
     CDS             complement(4912..6204)
                     /locus_tag="PK196_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HON41012.1"
                     /translation="MFWNRAMETIRGDDLLDLQIKRLKWSLHQAGKTGLYQRRMKEAG
                     VSPDDIKTLEDVERLPFTTKKDLQAGYPFGLFAVPMKEVVRIHTTSGTTGKPTVVGYT
                     RQDLENWSELIARNMTMIGLGEDDIFQNAVNYGLFTGGLGFHYGAEKIGMTVIPSATG
                     NTRRQIEMIEDFGVTAIHCTPSYALHLAEVAESMGKSLDTLKTGIFGAEPWSESMRGE
                     LERRLGLKAYDSYGLSEMYGPGVAFECPERDGLHIWHDSYLVEVIDPATGERLGPGER
                     GELVITPLVKEAMPLIRYRTGDITRLMDEDGCACGRGQKIERITGRCDDMLVIRGINV
                     FPSQIEHVLRTLPEVGDQFMVYIDRVKHLDEMTIEVEMSKVSFSGELQDLTRIQKKIS
                     AELQNTLGLRTAVRLVEPGSLPRFEGKARRVIDRRGNA"
     gene            complement(6361..7386)
                     /gene="wtpA"
                     /locus_tag="PK196_06040"
     CDS             complement(6361..7386)
                     /gene="wtpA"
                     /locus_tag="PK196_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HON41013.1"
                     /translation="MSRSAAFLAVLGLLIIFAAAAGCTWSEQERTPLKVVPAGSLLYP
                     FEQIEAEFENQHPEIDVQLEGHGSIQAIRQVTDLRRPIDVVAVADASLIPDMMFIPMG
                     EGEENYTDWYTPFAENEIVIAYTGRSRGADEITPENWYRTLARPEVRVGFSNPMLDSC
                     GYRALMVTALAEDHYNEPGLFEEIIGGAFDPPIRPQLQDGMITINLPERMRPATDKVA
                     IRDGSIYLLSLLDAGGIDYAFEYRSVAEEHGLHWIDLPPEINLGSAEHADYYGKVRVN
                     LGFQRFQSIGSERIGQPIVYAITVPRNAPHPEEAQNFVEFVIEAFRKERPGWPDPLSP
                     EMRAVSV"
     gene            complement(7383..7955)
                     /locus_tag="PK196_06045"
     CDS             complement(7383..7955)
                     /locus_tag="PK196_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HON41014.1"
                     /translation="MIPVRRRAYPALLILVSIGLLALAAGCLGTTETDDYPPWNLTLL
                     GDTEIVLSLDEIREMDAVEGYGYAVSTVGIRYGPNRYRGVLLTDLLDMVGGVGKDDLV
                     YVSAPDGYMWVFDAAQVRGEELFTFDENLHEIPSPPLQVILAYDCDGKPLAHDNGGPL
                     RLVVISETPDVITEGSPWVKWVDKIEVRRR"
     gene            complement(7952..>8353)
                     /locus_tag="PK196_06050"
     CDS             complement(7952..>8353)
                     /locus_tag="PK196_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HON41015.1"
                     /translation="WYNGGDPEVGERQGTGYVPDYYTGMRLVFFADNSTNPEGLHVYG
                     NWDMHETLPDAAQHFYDGLYPSTSGLTVKWVDEIRVYTGGYQGEKGALAGSLTTPEGT
                     AAEPTEAPLSLLVTVLAVAGAAILCARRGGA"
BASE COUNT         1557 a         2517 c         2414 g         1765 t
ORIGIN      
        1 ggcataccgc atgaagatga aggcagcatc ttcatacaga tcccggccac ccccccggaa
       61 tctgtccatg gactggacac ggagaggttt atcaaaccag tgaggcattg gtttgatggc
      121 ctcgttctga ccgctcaaca acatcatcgc tgaataaata agatcccttc catactatac
      181 tgcatggcaa tgcctactct tcaggtggcg ctcgacctcc ttgacctcga tcgtgcggta
      241 gagatcgcgg ctgaggccgt ccagggcggt gcggactgga tcgaggtcgg gacgcccctg
      301 atcaagagcg aggggatgcg ggctgtacgg acgctccggg aacgcttccc cgaccacgag
      361 atcgtcgctg acatgaagat cgccgacacc ggtgcgatcg aggtggagat ggccgcaaag
      421 tccggcgcag gtatcgtctg tatccttgcc gacgctgacg acaccgtgat tgcagaggcc
      481 gtccgctcag cccgcaagta cggcgtccgg ctcatggccg acctcatcaa cgtcagcgac
      541 cccgtcgccc gggcccgtga agttgcggag ctcggcgtcg actacatcaa cgcccacgtc
      601 ggcatcgatc agcagatgat cggcagatca tcacttgacc tcctccaccg ccttaccggc
      661 gaggtgaacg cccccatcgc cgttgccggg gggctcgatg cagagaccgc atccgccgcg
      721 gttgccgccg gggccacgat tgtcatcgtg ggcagcaaca tcatcaaggc cgccgacgtg
      781 accgcagcga cccgccgggt cagggaggcg atcgaccgcc ctgagattcg gccgcgggag
      841 aaggagagcc ccgacgccgt catccgccgt ctctttgagg cggtctctgc gccaaacgtc
      901 accgatgcca tgcaccggaa gggcgcgatg gcgggcataa tctcaatctg cggcagggtc
      961 agggccgtcg gcccggccgt caccgtccgg acagtttctg gagactgggc aaaacctgtc
     1021 gaggcgatcg atatcgccag gcccggcgat gtcctggtca taagtaacga cggccgaaag
     1081 gatatctcac cctggggtga acttgccacc cactccgcga agaaccgggg tcttgcaggc
     1141 atcgtcatcg acggcgcagt gagggacgtc gacgatatcc gcgagatggg gttccctgtc
     1201 tttgccacag caaccgtccc gaatgccggg gagcccgtgg gtcttgggga gatcaacacc
     1261 gagatcctct gctgcggcca ggaggtccga cctggcgact ggatcgtcgc tgacgagagc
     1321 ggtgtcgtgg tagtcccccg cgatcgggcc tacgagatcg cccgtcgggc gatggaggtc
     1381 cggaagaccg aagagcggat ccgggaggag atcaggcgcg gccggacgct ctcagaggtc
     1441 tcagaactcc ttaaatggga gaaacggcgc tgaaatgccc gtgatgagat aaccgtaaac
     1501 acgaggaatg tattcagatg cagaatataa gagaagaggt agttggggtt ttgcaggcgg
     1561 tctttccaat agtcgtcgtc atcatccttc ttgagttctt cgtgctccag gccacacctg
     1621 ccgatcttat ccagtttctg ctcggcagcg gtatggtgat cctggggatc gccctctttc
     1681 tcactggcgt caaaaccggg ctcctcccaa tcggcgaggc gatcggctcg gaactgcctg
     1741 ccaggggatc tcccacactg ctcatcgcgg ggtcgttcct ttttggtttc ctggcggcca
     1801 tagcagagcc aaaccttcgt gtcctcacaa ccactctcga caccatctca ggaggtatcc
     1861 cgtcaggtcc cctgcttttt gtgatcgcag tcagcgtagg gctcatactc accgccgcga
     1921 ccctccggat agtgtttggg tttcctctgg cctatctttt tgccgccgct tatggtgcta
     1981 tcattgtcct tgcaccgttc acggcacccg atttccttgc cgtcgcggtc gactccggcg
     2041 cgataacaac tggactgatg acgatcccgg taatcctggc gctcgggaca ggtgtcagta
     2101 gtgttctcac cgggcgttca cccctcacgg atggttttgg gcttgtcggt cttgcctcga
     2161 ttgctccaat catcagcatc atgctgctgg gggtgatata ctgatcgtag tggcgctctt
     2221 tgaggggatg gatcaggtaa tctttgaggc tgcccaggcc cttcttcccc tgcttatctt
     2281 ctttggggtg ttccagttgc tttacctgaa actcccccgc gtatatgtcg tgaaccttgc
     2341 aaaaggcatc ctgcttgcgt ttctaggcat gatcttcttc cttcagggcg tcgatgtcgc
     2401 gtttctaccg gcgggccggg agatcgggga agcaatcgcg gcgttcgacc agcggtggct
     2461 gctcatcccg ttaggatttt tcctgggttt tcttgtcacc tacgcagagc ctgaggtcag
     2521 ggttctcggc tatcaggttg agagatcatc ggacggatac atcagagaat cactcatcgt
     2581 ctacaccctt tcctttgcgg tcgcagtagc cgtctcgctc gggatggcac gtctcgttta
     2641 caacatccca ctgctgggca taatcatccc gggctacctc cttgtcatca tccttctcat
     2701 cttcaccgat cgggattttg tcgggatagc gtttgattca gggggtgtgg ccaccggccc
     2761 gatggcggtc actttcctgc tatctcttgc aatcggggtt gcgacgggga tcgaagggct
     2821 aaatcctatc acggacggtt tcggcctgat cgctctgata gcgcttgccc ccatcctctc
     2881 gatgctgctg cttggcaccc tctacaggag aacaaaaggt gaaaccacat gaacaatgat
     2941 tatggcaatg aactgatcgt cacgattgta aaacgtggct ggtcgcatac ggtggtcgat
     3001 gctgcccgca aagccggggc agaaggtgcg accattctct ttggccgtgg atccggcatc
     3061 aatgaggtaa agacccttct cgggatcaca attgaacctg agaaagagat cattttaacg
     3121 gtcgtaggcg ccgagcggac agatgcggtg ctcgatgcga tcctcgccgc cggcgaactg
     3181 gacaggccgg gaatggggat ggcgtttgtc gtcccgatcc ggcgtgttgc agggagggtc
     3241 catatgtttc gcaaaaatgg gggtgacaaa taaaacccgt aaaatcagcg atcttttccc
     3301 ggtagtatac gcaaaagacc atcaaaccag aaaatctggt ttggatagtc caaccgggct
     3361 atcccacgct cttcacggtg aatggggaat tttaagcagt tttccgccgt ggtccagtat
     3421 ctcacggaca gcatcctcga ccttgtccac gcgaaggatc aggacggccg catctttacc
     3481 ggagtatgca taggcatact ctatgttgat cccggcatcc ccgagcaccg aggctatctc
     3541 cgagagtcct ccgggctcat cgcgcatctt caccccgatg acatcggtga acgagacgcg
     3601 gaaaccgaga tcggtcaacg tccggtaggc atcgtccggc cggtctacaa gcgcccttac
     3661 aaccccgaac ccgttcgcct cggctatgct gaacgccagg atgttaatcc tggcgtcgcg
     3721 cagcgcagcg gcgattgccg caagccgtcc cggtcggttc tccgagaaga ccgagatctg
     3781 tttgatgata tacgacgact tctccattac aatgacctcc tgtctataac ctttctggat
     3841 ttgccctcaa accgcggaag agtcccgggt tcgaccagtt caacctccac gctcacgttg
     3901 agcgaactcc ggaggcggtg ctctaccgtc ctcttgatct ccatgagatc ggggatcctg
     3961 tcagagaaag cttcctcgct cacctccacc cgcacgagca tcgagtcgag cgcacccttc
     4021 cggtcgacga cgatctggaa atgcttcccg acctcgggta tctcaagcag cgtnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaacatcg ggcctgagag ttcgcttgtc
     4201 ccgtagatgt cgtaagcacg gatcccgagc cagtcctgga tccgcgttcg catctgctca
     4261 gaccaggtct cggcacccag gaaacccatc tggaggtcgg tgtcgtcccg tatcgagacg
     4321 cccatctttt ccgccacctc cgcgatgtgg aggaggtagg agggggtgca ggcgatggcg
     4381 gttacgtgga gatcctgcat cagttccacc tggcgctcgg tgttccccac gcttgtcggg
     4441 aggactgtcg ccccgagacg ctcggcgccg tagtgtgcac cgagccctcc ggtgaagagc
     4501 ccgtagccgt aactcacctg gagaacatcc ccccggccaa gaccgcagga ggagaacgcc
     4561 cgtgcaagag actcgctcca gtttgcgatg tcgcgctcgg tatacccgac gacggtcggc
     4621 tttcctgttg tcccggagga gacgtggtac ctgaccagtt cgctccgttc ggcggtgaat
     4681 atccggtcag gataaccgtc gcgcaggtct gacttgtgca taaacggaag tttcgcgaga
     4741 tcctcaagat tccttatatc atcggggtgg acgccgtgct ccttcatccg gcgccggtag
     4801 aactcgttaa aactgtagag ccggtatacc tgggatttga ggagcttgaa ctggagccgc
     4861 tggagttcat caatcggcat ctcctcgatc cgagggttcc atggcggcat ctcaggcatt
     4921 cccccgccgg tctatgacac gtcttgcctt cccctcgaac cgcggcagag accccggctc
     4981 caccagtcgc accgctgtcc gcagcccgag cgtgttctga agttcagccg atatcttctt
     5041 ctggatgcgg gtgaggtcct gaagttcgcc ggagaacgag accttgctca tctctacctc
     5101 gatcgtcatc tcgtcaagat gttttacacg gtcgatatat accatgaact gatccccgac
     5161 ctcgggaagg gtacgcagta catgctcaat ctgggagggg aagacgttga tcccgcggat
     5221 gaccagcata tcatcgcacc tgccggtgat ccgctcgatc ttctgcccgc ggccgcaggc
     5281 gcagccgtct tcatccatca atcgagtaat gtcgccggtg cggtagcgga tgagcggcat
     5341 cgcctccttg acaagcggtg tgatgacaag ttcaccgcgt tcccccggac cgaggcgttc
     5401 cccggtcgcg gggtcgatga cctccaccag gtaagagtcg tgccagatgt ggagcccgtc
     5461 cctctccggg cactcaaacg ccacaccggg cccgtacatc tccgagagcc cgtaactgtc
     5521 gtaggctttc agcccaagac ggcgttcgag ctcgcccctc atgctctctg accagggttc
     5581 agccccgaat attcctgttt tgagggtatc caggctcttt cccatcgatt ctgccacctc
     5641 ggcgagatgg agggcgtaac tcggggtgca gtggatggcg gttactccga agtcctcgat
     5701 catctcgatc tggcggcggg tgttcccggt cgcactcggg atcacggtca tcccgatctt
     5761 ctcggcgccg tagtggaacc cgagccctcc ggtaaagagc ccgtaattga cagcgttctg
     5821 aaagatatcg tcttctccaa gcccgatcat cgtcatgttt cgcgcgatca gttccgacca
     5881 gttctccagg tcctgccggg tgtagccgac aacagtcggc ttcccggtcg tcccggatgt
     5941 ggtgtggatc cggacaacct ctttcatggg gacggcaaag agcccgaacg ggtatccggc
     6001 ctggagatcc ttttttgtgg tgaagggaag tctctccacg tcctcaagcg tctttatgtc
     6061 gtcaggcgag acgccagcct ctttcatcct tctctggtat agcccggtct tcccggcctg
     6121 gtgcagtgac cacttaagcc gtttaatctg gagatccaga aggtcgtcgc cccggatcgt
     6181 ctccatcgcc ctgttccaga acatagttgg tatcccatcg gtcttttgcc ggcagaaggg
     6241 gcactctccc atctcccggc ggcgctactc cccggcggga tgccagagcc gcaccgccgg
     6301 gcgcagtctc tcttttaggt ttgggggcag tgcggaaaag gtttgtcaat ggatggcacg
     6361 ctacacgctg acagccctca tctccgggct gagggggtcg ggccatccgg gtctctcctt
     6421 gcggaacgcc tcaataacaa attctacaaa gttctgcgct tcctctgggt gcggggcgtt
     6481 gcgaggcacg gtgatcgcgt agactatcgg ctggccgatc cgctcgctgc cgatggactg
     6541 gaaccgctgg aaccccaggt tcacgcgcac ctttccgtaa tagtcggcgt gttccgctga
     6601 accgaggttt atctcggggg gcaggtcgat ccagtggagc ccgtgctcct cggcaacgct
     6661 ccggtactca aaagcgtagt cgatcccgcc ggcatcaagg agggagagga ggtagatgct
     6721 cccgtcccgt atggcaacct tatctgttgc gggtctcatc cgttcgggga ggtttattgt
     6781 gatcatcccg tcctggagtt gaggcctgat cgggggatcg aatgcaccac cgatgatctc
     6841 ctcaaagagc cctggctcgt tgtagtggtc ctctgcaagc gctgtcacca taagcgcccg
     6901 ataaccgcag gagtcgagca tcgggtttga gaagcccacc cgcacctccg gcctggccag
     6961 ggtgcgatac cagttctcgg gcgtgatctc gtctgcaccc ctgctacggc cggtgtaggc
     7021 gatcacgatc tcgttctcgg caaagggggt gtaccagtcg gtgtagttct cctcgccctc
     7081 gcccatcggg atgaacatca tgtccgggat gagggacgcg tcagcgactg cgacgacatc
     7141 gatggggcgc cggaggtcgg tcacctgccg gatggcctgg atactcccgt gcccctcgag
     7201 ctggacgtcg atctcggggt gctggttctc gaactccgcc tctatctgct caaacgggta
     7261 tagaaggctc ccggcgggga caaccttgag aggggtgcgt tcctgttccg accacgtaca
     7321 gcccgcagcg gctgcaaaga tgatgagaag gccaagcacc gccaggaagg ctgcactccg
     7381 gctcatcttc ttctgacctc gatcttatcg acccacttca cccaggggct cccttcggtg
     7441 atgacgtcag gcgtctctga tatgacaaca agcctgagcg ggccgccgtt gtcgtgggcc
     7501 aggggtttcc cgtcacagtc gtaggccagg atgacctgga gcggcggtga ggggatctcg
     7561 tgcaggttct catcgaaagt aaagagttcc tcacccctca cctgcgcagc atcaaagacc
     7621 cacatgtagc cgtccggcgc tgagacgtag acgagatcgt cctttccgac cccgcccacc
     7681 atatcgagca ggtcggtcag gagcaccccc cggtaacggt tcgggccata cctgatcccg
     7741 accgtggaga ccgcataccc gtaaccttcg acggcgtcca tctccctgat ctcatcgagg
     7801 ctgagcacaa tctcggtatc gccaagaagc gtcaggttcc agggcgggta gtcgtcagtc
     7861 tcggtcgtcc cgaggcaccc ggccgcgagg gccagaaggc cgatggagac cagtatgagg
     7921 agggcggggt atgcccggcg gcgcaccggg atcacgcccc tcctctcctg gcgcagagga
     7981 tcgctgctcc agcgaccgcg agaaccgtca ccagcaggga gagcggggcc tctgttggtt
     8041 cggcagccgt cccctccggg gtggtgaggg atcccgccag ggctcctttc tcaccctggt
     8101 agccaccggt gtaaaccctg atctcatcca cccacttcac cgtaagaccg cttgtcgagg
     8161 ggtagagacc gtcgtaaaag tgctgggcgg catcagggag cgtctcgtgc atgtcccagt
     8221 ttccatagac gtggagccct tcagggttgg tggagttgtc cgcgaagaag acaagcctca
     8281 tcccggtgta gtagtccgga acgtagcctg tcccctgcct ctcgccgacc tcaggatcgc
     8341 cgccgttgta cca
//
LOCUS       DAOSZX010000064         8353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7279_length_8353_cov_19.941432, whole genome shotgun sequence.
ACCESSION   DAOSZX010000064 DAOSZX010000000
VERSION     DAOSZX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7279_length_8353_cov_19.941432"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..901)
                     /locus_tag="PK196_06055"
     CDS             complement(257..901)
                     /locus_tag="PK196_06055"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HON41016.1"
                     /translation="MEPEEQNRMPVIGEPAPEFEALTTHGPLKLTDFRGKWVILFSHP
                     ADFTPVCTTEFIAFTAIADELARLNVQLIGLSIDSVHSHLAWVRSIKEKMGVEIPFPV
                     IADLDMKVARRYGMIHGKEGTTATIRTVFFIDEKGILRAMIYYPASNGRSMPEILRLI
                     KALQTSDAHGVATPANWQPGDKVVVPAPKTPEEIERRLKEGYECKDWYLCFKQI"
     gene            complement(905..1255)
                     /locus_tag="PK196_06060"
     CDS             complement(905..1255)
                     /locus_tag="PK196_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HON41017.1"
                     /translation="MDEKKLEEKIGKVPVIFKDLKETDPDLYAKVMGLDQVIWDDGAL
                     SRQTKKVIAIAIAAALRDGHAVRAQMAGAANLGVSKEEIEEGLRVAFLLAGMPAYVHG
                     KTALEEYFGKRAQK"
     gene            complement(1261..1386)
                     /locus_tag="PK196_06065"
     CDS             complement(1261..1386)
                     /locus_tag="PK196_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HON41018.1"
                     /translation="MVNVSAEGQVYRCEICGNVVQVLEVGGGELVCCGEPMVLEE"
     gene            complement(1398..1640)
                     /locus_tag="PK196_06070"
     CDS             complement(1398..1640)
                     /locus_tag="PK196_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41019.1"
                     /translation="MAEPETKKTAKAGEKPGPGRYVCIDCGRELKLDSTDQDLVKCPT
                     CACEMYNCLPMTHIRPDIKTPEDVMNPPERKKPGKS"
     gene            1745..2356
                     /locus_tag="PK196_06075"
     CDS             1745..2356
                     /locus_tag="PK196_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON41020.1"
                     /translation="MPGKVVALLGSPLLDGNTAYLLDEAIRGAEEAGCEVEKIEVAHL
                     DIMPCMEYFQCMGSENCLIEDGMERYFKMFREMDGLIIATPVMTMGIPGRLKSFMDRF
                     QVFYMAKYHRGRSFITPERRKKRRMLFISIAGMNLPSVFDGAKATARAFGEIIDCPYR
                     DEVLQNDMDTIRDIRTRPEVVEAAYRKGFELGRLLGEESEGSM"
     gene            complement(2296..>3131)
                     /locus_tag="PK196_06080"
     CDS             complement(2296..>3131)
                     /locus_tag="PK196_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HON41021.1"
                     /translation="AKAVFGFLAGSASMVADAVHSGFDSFSNVVGITALYFAGKPPDT
                     GHPYGHGKIETLGTQVIGLMLLLTAAGIIYGGVQRLVEPVIPDITVVTVSVMFGTLII
                     NIGVSTYERKKGLEYGSQILVADSRHTQSDVFVSLAVLGGFLAVRLGYPQADPIIALG
                     IGLLIARMGFRVLYEAAEVLTDTMNIPCDPELIRTVVMETPGVTGYHEFRCRGKPDEI
                     LADIHVTVDPETPVFQAHEISAEVERRLKEAVPGLADVVVHIEPSDSSPSSRPSSKPF
                     R"
     assembly_gap    3132..3231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3545..4045
                     /locus_tag="PK196_06085"
     CDS             3545..4045
                     /locus_tag="PK196_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HON41022.1"
                     /translation="MSTEENAQKAFAGESQANRKYSAFAEKAGAEGYSTVAKIFKAAS
                     EAEAIHAKRILFLLDAVGTTEENLKTAMEGENYEFTEMYPAFVAEAKKERKNEASIVF
                     THAMRAEEVHANIYLQALEAVRDGKDLDAEKVFLCPVCGNIELGTAPEKCPICGVPQR
                     MFREIQ"
     gene            4127..5308
                     /locus_tag="PK196_06090"
     CDS             4127..5308
                     /locus_tag="PK196_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON41023.1"
                     /translation="MAGVKVYTTESCPYCRMVQAFLRKYGIDYEVVDVGKDREAAREM
                     IRISGQRGVPVTVVGDEVIVGFDAKRLREVFGTAAIDRVYDTVIVGAGPAGLTAAVYS
                     ARKLLKTVVVSEDIGGQAAWSWMVENYMGFTAITGEELVRKFEEQVRVFDVSLELDSV
                     QSIARESDLFLVRTASGNVFKCRTVILAPGKEPMTLGLPGETRLMGRGISICATCDAP
                     LFRGMPVAVVGGGNTAAQTAIEMAKIASSVAMIVRSDLRCDEVYVNRAKELGVQIYLH
                     HEVTALHGQDALTGITIRDRETRKETVLNVAGLFLAIGLMPKTGFLGGLVALNEMGEI
                     LIDVNTHTNVPGIFAAGDATCVRAKQIIVAAGEGAKAAIEAHDHILQQGLSSNNNVQG
                     R"
     gene            5342..6250
                     /locus_tag="PK196_06095"
     CDS             5342..6250
                     /locus_tag="PK196_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41024.1"
                     /translation="MTLEEALRVRGDRDLPSIRAAFKEAGMPAQVRDVATILDEMIFV
                     MGTIGCIKSLLKERLAQLEEEDEDTGSLQTTNLEIVEKVAGIIAGIMERYKPGDPLPG
                     TGFAGMSWETGRQTPWKDEMQWFIAISTLKGNGLAREEDGRVILRKRADPESLVMALP
                     EDIIEGIDPEILKDRGITANMTVSSVPEYRLVFEPEAILEADLENIDDLAVQLDLDPD
                     TYAAFREAVFLKQLVAARVQEIIEERGTLTPEEVAEILKKSVVEDPDGIWTISLNLST
                     EFVKGVLEDLRKIGLLSKKGTGFRAV"
     gene            6446..>6642
                     /locus_tag="PK196_06100"
     CDS             6446..>6642
                     /locus_tag="PK196_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HON41025.1"
                     /translation="MIEDTLRGMTNGMLARTAGLISRTGVTPNAITVAGFIGMAAAGL
                     LCARGSFAAAGLLVAASSVFDA"
     assembly_gap    6643..6742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6743..7203
                     /locus_tag="PK196_06105"
     CDS             <6743..7203
                     /locus_tag="PK196_06105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HON41026.1"
                     /translation="GSFAAAGLLVAASSVFDALDGALARTTGATTPFGAFLDSFLDRY
                     AEAAVYAGLLVYYAGASTPWGVEAAFAAAIGSLMVSYARARAEGIGVDCRAGLFARAE
                     RIVVLIVGLLTGLVLPALVILAIATNATAIERLILVRRATLAPPRPPRSP"
     gene            7334..8344
                     /locus_tag="PK196_06110"
     CDS             7334..8344
                     /locus_tag="PK196_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HON41027.1"
                     /translation="MSCSIIVGGFFGDEGKGKIVAHIAHHDRPSIISRGGVGPNAGHT
                     VKIGEKEYGVRMVPSGFVYPDATLMIGSGVLVDPRVFLHEIDLVGVRDRIFIDGRCGI
                     IEEEHVARDRGSEHLRDRIGSTGTGCGPANADRVLRLGRQAKDLPELSDFITDVAERI
                     NLALDNDEVVLLEGTQGFGISLYFGTYPFVTSKDTSASQIAADNGVGPTRIDDVIVVF
                     KAYPTRVGEGPFPTEMSMERSHKLGIEEFGTVTHRQRRIGTWDGKMARYSAMINGCTQ
                     AAITGIDRVDPACFGVTDYSKLTKPAMEFIEQAEEDIGRPVTLISTGPELSQIIDLRG
                     EV"
BASE COUNT         1738 a         2320 c         2476 g         1619 t
ORIGIN      
        1 tctcaccccg gaaccctctt tcctgcgtga gcgcggacgg cgggatgcga gaaccgtcgc
       61 ggaaatcccg ggggtgacag agaaagagga gttgagagga tggaggagtc acctcgaaaa
      121 ggtggcgctc gaagaaaact caaaacaagt cgtgtaaaac gccctgtttc ggcaaccacg
      181 gcagataacg gggctgagcc tggtgggttt tctgtctcga tatagacctg gacgaatgct
      241 acaaagaagt gggtttttag atctgcttaa agcagaggta ccagtccttg cactcgtaac
      301 cttccttcaa ccgtctctcg atctcttcgg gggtctttgg agccggcacg acaaccttgt
      361 ctcccggctg ccagttcgcc ggtgtcgcga caccgtgcgc gtccgaggtc tggagggctt
      421 ttatgaggcg gaggatctct ggcatggacc ggccgttgga cgcagggtag tagatcattg
      481 cacgaaggat ccctttctcg tcgatgaaga agaccgtcct gatggtcgcg gtggtgccct
      541 ctttgccatg gatcatcccg taacgccggg cgaccttcat gtcgaggtcg gcgatgaccg
      601 ggaacgggat ctcgaccccc atcttctcct tgatgctccg aacccacgca aggtgcgagt
      661 ggacgctgtc gatggagaga ccaatgagct ggacgttcag cctcgcaagt tcgtcggcga
      721 tagcggtgaa cgctatgaac tcggttgtgc agaccggcgt aaagtcggcc gggtgagaga
      781 agagtatgac ccattttccc ctgaaatcgg tgagtttcag ggggccatgg gttgtcagcg
      841 cctcaaactc cggcgccggt tcgccgatca caggcatcct gttctgttct tcaggctcca
      901 taactcactt ctgtgcgcgt ttgccgaaat actcctcgag ggcggtcttg ccgtggacgt
      961 aggccggcat ccctgccagc aggaacgcaa cccgcagtcc ctcctcgatc tcctccttcg
     1021 atacaccaag gtttgccgcc ccggccatct gagcgcggac agcgtgccca tcccggagcg
     1081 ccgctgcaat cgcgatcgct atgaccttct ttgtctgcct tgagagggcg ccatcatccc
     1141 agatcacctg gtcaagcccc ataacctttg catagaggtc tggatcggtc tccttgagat
     1201 ccttaaagat cacgggaacc tttcctatct tctcctcgag tttcttctca tccatctcaa
     1261 tcactcctca agcaccatag gttcgccgca acagaccagc tcaccgccgc cgacttccag
     1321 cacctggacg acgtttccgc agatctcgca gcggtaaacc tgaccctccg ccgatacgtt
     1381 caccattcat ctcaccttca ggatttccct ggcttcttcc tctcaggagg attcatgaca
     1441 tcctccggcg tcttgatgtc cggccttatg tgggtcatcg ggaggcagtt gtacatctca
     1501 caggcgcagg tggggcactt gacgagatcc tggtctgtgc tgtcgagctt gagctcgcgc
     1561 ccgcagtcta tgcagacata cctgcccggg cctggcttct ctcctgcctt tgctgtcttt
     1621 ttcgtctctg gttcagccat tgatattcac cagagcgata attaccgcgc aaggtattta
     1681 gcagttcccc tgctcatggg ggcatccgca tcctattaat aggctcgcgc gcaaccctcc
     1741 actcatgcca gggaaggtag tcgcactgct gggaagcccg ctccttgatg gaaacaccgc
     1801 ctatctcctc gatgaagcca tcaggggcgc agaggaggcg ggttgcgagg tcgagaagat
     1861 cgaggtcgct cacctcgata tcatgccatg catggagtac ttccagtgca tggggagtga
     1921 aaactgcctg atcgaggatg gtatggagag atacttcaag atgttccggg agatggacgg
     1981 gctgataata gccacccccg ttatgacgat gggcatcccg gggagactca agtcgttcat
     2041 ggacaggttc caggtcttct acatggcgaa gtaccaccgt ggccgctctt tcatcacgcc
     2101 cgaacggcgg aaaaagcgca ggatgctctt catctcaatc gccgggatga acctgccgtc
     2161 cgtctttgac ggcgcaaaag caaccgcacg ggcgtttgga gagatcatcg actgtcccta
     2221 ccgggacgag gtgctccaga acgacatgga cacgatccgg gacatacgga cacgcccaga
     2281 ggttgttgag gcggcctacc ggaagggttt tgaactcggc cggctgctgg gggaggagtc
     2341 tgaaggctcg atgtgaacga caacgtcggc aagccccggc acagcctctt tgagtcggcg
     2401 ctcaacctcg gccgagatct cgtgcgcctg aaagaccggt gtctcggggt ccacggttac
     2461 gtggatgtcg gcaaggatct cgtcgggttt gccacggcac cggaattcgt ggtagccggt
     2521 gacgccgggc gtctccatca ccaccgtccg gatgagttcg ggatcgcagg ggatgttcat
     2581 cgtgtcggtg agaacctctg cggcctcgta gaggaccctg aaccccatcc tggcgatcag
     2641 gagaccgata ccgagagcga tgatgggatc ggcctgcggg tagccaagcc tgacagccag
     2701 gaacccgcca agaaccgcca gcgaaacaaa gacatcactc tgggtgtgtc gcgagtcggc
     2761 gaccaggatc tggctcccgt actcaaggcc cttcttccgt tcgtaggtgg agacaccgat
     2821 attgatgatg agcgtcccga acatgacaga gacggtgacc actgttatat ccgggatgac
     2881 gggctcgacg agtctctgga cgcccccgta gatgatgccg gcggcggtca ggaggagcat
     2941 caggccgatc acctgggtgc cgagcgtctc gatcttgccg tggccgtagg gatgcccggt
     3001 gtcagggggt ttcccggcga agtagagtgc ggttatcccg accacgtttg agaaagagtc
     3061 aaacccggag tgaacggcat ccgccaccat gctcgccgaa ccggcgagga agccaaagac
     3121 agcttttgca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcatccgcc
     3241 accatgctcg ccgaaccggc gaggaagcca aagacagctt ttgcaaacgc aactgtgaga
     3301 ttcagaacaa gggtgaggat taatatccgc cgcacctgga tctggacctc cggtcttttg
     3361 cctcctggcc gctggcgggt cgcatcctcc cccatacacc atgataagta tgcgagcggt
     3421 atagggtttc cgaaaagacc gcatgaatcc accgggctac aggcttaccg atctggagat
     3481 caaatgttat tcccatataa cgagggtgaa agatttaata agcgcgtcca gaaaagagtt
     3541 catcatgtca acagaagaaa acgctcagaa agcgttcgca ggcgagtccc aggcaaaccg
     3601 gaagtactca gccttcgctg agaaggcagg cgccgagggc tactcaacgg tggcaaagat
     3661 cttcaaggcg gcaagcgagg ctgaagccat ccatgcaaaa cggatcctct tcctcctgga
     3721 cgccgtggga accacagagg agaacctgaa gaccgcgatg gagggtgaga actacgagtt
     3781 cacggagatg taccccgcgt ttgtagccga ggcaaagaag gaacgcaaaa acgaggcctc
     3841 gatcgtcttc acccacgcga tgcgtgccga agaggtgcac gcaaacatct acctccaggc
     3901 gctcgaggcc gtccgggacg gaaaagacct ggatgcggag aaggtcttcc tctgccccgt
     3961 ctgcggcaac atcgaactcg gcacggcacc ggagaagtgc cccatctgcg gtgttccgca
     4021 acggatgttc cgcgagatcc agtaacccct cttcttcttt tcaaccagat ctcacaccgc
     4081 tctctcccac gcaagttaaa tactctttca gtgttatttc ttcaccatgg caggcgtgaa
     4141 ggtctacacg actgagagct gtccctactg ccggatggtt caggcgttcc tccggaagta
     4201 cggcatcgat tatgaggtcg tagatgtagg gaaggaccgc gaggcggcgc gggagatgat
     4261 ccggatctcg gggcagcgag gggtgccggt cacggtggtc ggcgatgagg tgatcgtagg
     4321 gttcgatgca aagaggctcc gcgaggtctt cgggacggca gcgatcgacc gggtctacga
     4381 tacggtcatc gtgggtgccg gccccgccgg gctgacggcc gcggtctact ctgcccgcaa
     4441 actcctcaag acggtcgtgg tctcggagga tatcggcggg caggctgcct ggagctggat
     4501 ggtcgagaac tacatgggct ttacggcaat cacgggcgag gaactcgtcc gtaagttcga
     4561 agagcaggtc agggtgttcg atgtcagcct tgaactcgac agcgtccaga gtatcgcaag
     4621 agagagcgat ctctttctcg tcaggacagc ctccgggaac gtattcaagt gccggacggt
     4681 catcctggcc cctggaaaag agcctatgac gctcggcctc cctggtgaaa caagactgat
     4741 gggacgcgga atctcgatct gcgcgacctg tgacgctccg ctcttccggg gtatgcctgt
     4801 tgccgtcgtc ggcggcggga acactgccgc ccagaccgct atcgagatgg cgaagatagc
     4861 aagttccgtg gcgatgatcg tcaggagtga cctccggtgc gacgaggtct acgtcaaccg
     4921 cgcaaaggaa ctcggtgtgc agatctacct ccaccacgag gtgacggcac tccacgggca
     4981 ggatgccctg accgggatca cgatccgcga cagggagacc aggaaggaga ccgttctcaa
     5041 cgtcgcaggg ctgttcctgg ccatagggct catgccaaag acagggttcc ttggcggcct
     5101 ggtggcgttg aatgagatgg gcgagatcct gatagacgtt aacacccaca caaacgttcc
     5161 cgggatcttt gccgccggag atgcaacctg cgtccgggcc aagcagatca tcgtcgccgc
     5221 cggtgagggg gcgaaggcgg cgatcgaggc gcatgaccac atcctgcagc agggactctc
     5281 cagcaacaac aacgtgcagg gcagatgaac cgcaccgcca atgtgggtgg agaaaaaaaa
     5341 gatgacgctt gaagaggcac tccgtgtccg cggggatcgt gaccttccca gtatccgtgc
     5401 ggcctttaag gaggccggta tgccggcaca ggtcagggat gtggccacca tcctggatga
     5461 gatgatcttt gtaatgggga ctataggatg catcaaatcg cttttgaaag aacggctcgc
     5521 gcaactcgag gaagaagatg aggacaccgg aagcctgcaa acgaccaacc ttgagatcgt
     5581 ggagaaggtc gccgggatca tcgccgggat aatggaacga tataagccgg gcgatcctct
     5641 acccgggacg ggttttgccg ggatgagctg ggagacagga cgacagactc cctggaagga
     5701 tgagatgcag tggttcatag caatctcaac gctgaagggg aacggcctgg ccagagaaga
     5761 ggacggcagg gtgatcctgc ggaagcgtgc agacccggag agcctggtga tggctctgcc
     5821 ggaggatatc atagagggta tcgaccccga gatcctcaaa gaccgcggaa tcactgcgaa
     5881 catgaccgtc tcgtcggtgc ccgaataccg gctggtcttt gagcctgaag cgatacttga
     5941 agcggatctc gagaatatag acgatcttgc agtgcaactc gatctcgatc ctgacaccta
     6001 cgcggcgttc cgtgaggcgg tcttcctgaa acaacttgtt gccgcgaggg tgcaggagat
     6061 catcgaggaa cgcggaaccc tgactccgga ggaggttgca gagatcctga aaaagagcgt
     6121 tgtagaagat cccgacggaa tatggacgat atcgctgaat ctctccacgg agtttgtgaa
     6181 aggcgtcctt gaggacctca ggaagattgg cctgctaagt aagaagggca ccggattccg
     6241 cgcagtctag acctgccggc gccatgaccg aaatttcgtt acagccccac ctgactatag
     6301 agtccaccca cgaatggctc tacgatacgc atcagggagc ggcaagactc aacaggcacc
     6361 ttttttttat caccagggag cgaagatctc tcctcatgtt gcggccgttg agacgccctg
     6421 atcgcttcat gggggtgagg tgagcgtgat cgaggatacc cttcgcggaa tgacgaacgg
     6481 catgctggca cggacggcgg gcctaatcag ccggacgggg gttaccccga acgctatcac
     6541 ggtggcgggg tttataggga tggcggccgc gggtctcctc tgtgcgcggg gatcgttcgc
     6601 cgccgcgggc ctcctcgttg ctgcatcctc tgtctttgac gcnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnggggatcg ttcgccgccg cgggcctcct cgttgctgca
     6781 tcctctgtct ttgacgccct ggacggggcg cttgcccgga ctaccggcgc aaccaccccc
     6841 ttcggcgcct tccttgactc gttcctggac cgctacgctg aggctgcggt ctatgccggg
     6901 ctgcttgtct actacgccgg cgcctcaact ccctgggggg ttgaggctgc gtttgccgcc
     6961 gctataggct cgctgatggt gagttacgcc cgcgccagag cggaggggat cggtgtagac
     7021 tgccgggccg gtctcttcgc ccgtgcggag cggatcgtgg tcctgatcgt cgggctcctg
     7081 accggtcttg tcctcccggc tctcgtaatt ctggcgatcg caacgaacgc caccgcgatc
     7141 gagcggctca tccttgtgag gcgggctacg ctcgccccgc cacgaccgcc gaggtcaccc
     7201 tgagcgtttc aggtaatttt ctccagccgt gaaagactct cgtaaacgcg aaaaatgcaa
     7261 ttaagcacca cgttgaccgg gcacattcga tgtttttatc agcatgtatc gctctaccct
     7321 acttgataga agaatgagtt gcagtattat cgtcggcggg ttctttggtg acgagggcaa
     7381 aggaaagatc gtggcccaca tcgctcatca cgatagacca tccatcatct ccagaggggg
     7441 tgtaggtcca aacgctggac ataccgttaa aattggggag aaggagtacg gtgtcaggat
     7501 ggtcccatcg gggttcgtat acccggatgc aaccctcatg atcgggagcg gcgtgctcgt
     7561 cgacccccgg gtcttccttc atgagatcga cctggttggt gtccgtgaca ggatcttcat
     7621 cgacggtcgc tgcggcatca tcgaggagga gcatgttgca cgcgacaggg ggagcgagca
     7681 cctcagggac aggatcggga gcaccgggac aggttgtggc ccggcaaacg ccgaccgcgt
     7741 cctccgtctg ggcaggcagg caaaagatct cccggaactc tctgatttta tcaccgatgt
     7801 tgcagagagg atcaacctgg ctctggacaa cgatgaggtg gttctcctgg aagggacaca
     7861 gggcttcggg atctctctct acttcgggac ttacccgttt gtaacgagca aggacacctc
     7921 cgcctcgcag atcgccgccg acaacggtgt cggtccgacc cggatcgacg atgtcatcgt
     7981 cgtcttcaag gcctacccga cccgggtcgg tgaggggccg ttccccaccg agatgtcgat
     8041 ggaacggtcg cacaaactcg ggatcgagga gttcggcaca gtgacacacc gccagcggcg
     8101 tatcggcacc tgggatggga agatggcccg ctactctgcg atgatcaacg gctgcaccca
     8161 ggcggcgatc accggcatag accgggtcga ccctgcctgt tttggcgtaa cggactacag
     8221 caagctgaca aaaccggcga tggagttcat cgagcaggca gaagaggaca taggcaggcc
     8281 tgtaaccctg atctcgaccg ggcccgaact ctcccagatc atcgatctga ggggtgaagt
     8341 atgagaaaaa acc
//
LOCUS       DAOSZX010000065         8211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7431_length_8211_cov_19.028077, whole genome shotgun sequence.
ACCESSION   DAOSZX010000065 DAOSZX010000000
VERSION     DAOSZX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7431_length_8211_cov_19.028077"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3056
                     /locus_tag="PK196_06115"
     CDS             <1..3056
                     /locus_tag="PK196_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HON41028.1"
                     /translation="NVVALAEDDEVVFCYGSDPTPETATALITITVGLEDSDETPLEP
                     ATWSVSLVGARTEEVDQDYFEDGVNCGHDATYTDDNGDVWSGMPLWYLVGLVDDDLRH
                     GSGAFNDDLAAEGYSIKVTSSDDYSINFDSASVARNDGIIVANTLNGEPLPATIGDKN
                     KPCWPLRIVGPSVSSGQLIGGVTTIELIDLPEASEGWEITLVGAFNRTLTQAEFEEGA
                     ECHGKSYTDDKDQVWTGIPLWYLAAVVDDVATGNHWTLNDTRATAGYTVRVSAADGFN
                     ATFSSADIARNDSFIVANKMNAAPLTEDNGAPLKLVGTGVTSGKQKIGKIATITLEGL
                     PGEVTDSEWTLRLEGPKITDILTKEEFEECGHHVATYNDGVSTWSGVPLKVLCGWVDD
                     DVMHGSGAFNNALALAGYTVIVSSGGDNPYSKEFQSQDILASPNNYIVANKVNGTPIT
                     GSAYPLQLVGAGAAGSLSVKNVQRIQLVDFQEPEEPPSIRVVRYASDGVTVVNETTKT
                     IQWMEENLEVYGAPDGVRLRFQGPTFDPDDLWNPAEDINVDPAKVDDVVKGTAIRDLC
                     DLVGGAPEGGEIELIASDGYKVKLNYTNIYEPLGRQGEAIVAWWAEGRGYVPDYRDGP
                     RLFFNAPDGIFGNDDMRTCLAEDYWHYYWDGGIQYPSAAGLANKNIATIAIKPGARED
                     WTLLLRGAITDTITRSYFESGKACTMGGHGATWTDDEGHVWSGMPLWFLCGWVDDGNK
                     HDYGTNPFRDDLAAAGYNVTVIDYGADGTKGTDDDFSATFNSSFVARNNNIIVADEID
                     GAPLPKDGKTWPLKLVGSALTSNKQRVGSIDEIVLTGVPIVEEPPEANATIDLEEGWN
                     FVSTPKRLMDGSDTFETIYGNVDTAGHSILLYDGLTQEWTAVTSNETFKPLDGVWVYA
                     NETCTVPLTFAPAGPETPPTKNLGEGWNAIGFTDTVPESAANTLLSLGDHWTTLIGFD
                     AGSQEYEVSIVRGATGRHGEERTMEPMQGYWVYMTGADMLAAIGA"
     gene            3068..>3816
                     /locus_tag="PK196_06120"
     CDS             3068..>3816
                     /locus_tag="PK196_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41029.1"
                     /translation="MRLKQHGIVLLLLLGLVAGAAAATEGSPPVLPHEFYGNVMIGGS
                     AAPAGTEITATIDGVGCGSVVTTEAGRYGSPDPSLGNRLIVTAAADQEGAPITFLVNG
                     RAAKETANFTSDAVTVLDLSVAAEATPTTPPGGSTGGGGGGGRGTSGSPGSEVVYEPE
                     APTAPVIFTKEATLTASTAGVVLEPVVVRTEDEVAALTIPEGTTALDAGGNPLTSVTC
                     TKVDPAEVPPAPPGASLAIALSCGPDGATFDP"
     assembly_gap    3817..3916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3917..6780
                     /locus_tag="PK196_06125"
     CDS             <3917..6780
                     /locus_tag="PK196_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HON41030.1"
                     /translation="ALCLLLALAAPAAMAATTELHVVRYAADGVTILNETTVDYRWLE
                     SNLPVQGDGVTHYYHQGPVFEGDKWNPEEDTNVLEKDMGAVKGTDLKDICDLVGGMKE
                     GETVRIKASDGLSRVFPYRNVYEPEPRQGPMVITWYHDEDGYVPDYYSGMRLVFFADT
                     STNPYGVHAFGVWDMHECFDEEYWYFYGGEYPTTTGLSVQYISEILIYSEEEPTGGIH
                     VNSTPSGATIILDGEDTGYETPYTLTGIESGSHSILVEKGGYVQPDERWVTVGANQIT
                     EVEFNLTPITGKIAVSSVPINASIILDGEATGLYTDTVMEEVPVGEHTIELVLPGYRN
                     ATRTVVVEEDEYSILDLVLIPVNESVPGTLPEASMNATPGNATLFSLLGPFEFHGRVD
                     VLPANISTTPSGSVVSYRLPDGAKGRGYIYIDNRSAGEPDFEGFNPDRRYTAAAAATF
                     AANLTGENSTTFTAHCPPGVIPGGLLLTVEVDENASSIICWIGEGLLIPDGERPLVFE
                     PAPDLSRISAARLLIVGTGNITARFNGHSITGIPQGEPAVTEYDVLACLNESSNELRL
                     EGEDAVIRNVILSLTTTDGMPEPSPTPVEARKSLIERLLGLLAGIFGFSTEEPEQNTT
                     RDLPEVEETAVPEETRPTPAVTPTVNRAVKNHSGGVYITSYPPGMTIIVDNKKLSRQT
                     PRVIYGLREGLHTIQVEASTTYPGQEEPGYRFETVQAWVYPDAITPVHLDGVAPSSRK
                     TVRVESEAYRGERFTLNGIFRAGTIPGEGEIEGGAAWVTVLHEGRYLSSSLPTPVEDG
                     MRFVVEPWTGETVSLSVRSNPPGALVFIDGFPTDERTPCRMDGLSPGRHRILVSMPGY
                     LPAEEVITIPEGSRNGGVITCTLQEYTSGGLLVESNVPDARIYLYGRYTGEKTPHTFT
                     GMSIGTYEVRVVSENDSRTVEDVLVKAGATTRCLVTLER"
     gene            6788..7963
                     /locus_tag="PK196_06130"
     CDS             6788..7963
                     /locus_tag="PK196_06130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HON41031.1"
                     /translation="MKRYCTLLATATAALLLIQAASAGIVWGPYVTNTTSNSTVVSWK
                     ATGDTGGWVEYAAVEGGETNRIPSSGTEGGIQHAHLSDLSPATTYRYRVVSGNETTGD
                     CRFRTFGDLEFTCIIYGDTRGQKPLFTQADRHRLVAERIAEEEEFLFLLHTGDFVCDD
                     SEWDEFFSVVGEALNKTTLVPVAGNHDGTAEAFSRIFAVPTDYSFDAGSLHVTVLDSN
                     DRAWADMANRTAWLKEDLRSPLPRKIVAFHHPPFSADRKRPGGDLGIRAEWAEIFSEN
                     GVDAVFSAHTHAYERYSVNGTDYFVIGCGGAPFYPLSDEKPEGYRMGCEETLGYLRAR
                     VSPESIIIEMIPVAKVIDDEVVLYPRGFVIETVRLPAGWWSVFPSVAGVTRLPELWR"
     gene            7960..>8211
                     /locus_tag="PK196_06135"
     CDS             7960..>8211
                     /locus_tag="PK196_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HON41032.1"
                     /translation="MMDLHAEIRRILLALLCVSILVIGAVSAAPTTELRIVKIGADGV
                     TVLNETTVDYRWMEENLPVLGDGVTHYYHQGPVFEGDKWD"
BASE COUNT         1745 a         2505 c         2457 g         1404 t
ORIGIN      
        1 tcaacgtcgt cgcccttgcc gaagatgatg aggttgtctt ctgttacggc agtgacccga
       61 cgccggagac cgcaacagcg ctgatcacga tcaccgtggg tctcgaggac tccgatgaga
      121 cacctctcga gccagccacc tggagcgtca gcctcgtcgg tgcacgaacg gaggaggtcg
      181 accaggacta ctttgaggac ggggtgaact gcggacatga cgccacatac accgacgata
      241 acggtgatgt ctggagcggg atgcccttgt ggtacctcgt cggccttgtc gatgacgatc
      301 tgaggcacgg ctccggcgct ttcaacgacg acctcgccgc agagggctac tcgatcaagg
      361 tcacctcaag cgatgactac tccatcaact ttgatagcgc aagcgttgcg aggaacgatg
      421 gcatcatcgt cgcaaacacc ttaaacggcg agccgctccc ggcgaccatc ggcgataaga
      481 acaaaccctg ctggccgctc cggatagtcg gtccgagcgt cagttcgggg cagctgatcg
      541 gcggcgtcac cacgatcgaa ctcatagatc tccccgaagc ctccgaaggc tgggagatca
      601 cgctcgtggg cgctttcaac cggacgctta cccaggccga gttcgaggag ggtgctgaat
      661 gccacggcaa gagttacacc gatgacaaag atcaggtctg gacgggtatc ccgctctggt
      721 atcttgcagc cgttgtggat gacgtggcga ccgggaatca ctggacgctg aacgacacac
      781 gggcaaccgc gggctacacc gtgcgcgtga gcgcggccga cgggttcaac gccactttca
      841 gcagcgccga tatcgcccgg aacgactctt tcatcgttgc aaacaagatg aacgcagccc
      901 cgctcaccga ggataacggc gccccgctga aacttgtcgg gactggcgtg acctccggaa
      961 aacagaagat cggcaagatt gccaccatca ccctagaggg cctccctggc gaggtgacgg
     1021 actctgagtg gacgctccgc ctggaggggc caaagatcac cgatatcctg accaaggagg
     1081 agttcgagga gtgcggccat cacgtggcga cgtataacga cggcgtgagc acctggagcg
     1141 gcgtcccgtt gaaggtcctc tgcggctggg tcgatgatga cgtcatgcac ggttcagggg
     1201 ccttcaacaa cgcccttgca ctggccggct acacggtgat cgtctcatcc ggcggggaca
     1261 acccctacag caaggagttc caaagccagg atatcctggc aagcccgaac aactacatcg
     1321 ttgcaaacaa ggtcaacggc accccgatca ccgggagcgc ctatccgctc cagctcgtcg
     1381 gtgcgggagc ggctggaagc ctgagcgtga agaacgtcca gcgtatccag ctcgtcgact
     1441 tccaggagcc ggaggagccc ccgtccatcc gggtcgtccg ctacgcctcc gacggtgtga
     1501 ccgttgtaaa cgagacgaca aagaccatcc agtggatgga ggagaacctc gaggtctacg
     1561 gtgcaccaga cggtgttcga ctcaggttcc agggcccaac ctttgatcca gacgacctct
     1621 ggaaccctgc agaagacatc aacgtagatc ccgccaaagt cgatgacgtc gtgaagggaa
     1681 cggcgatccg cgacctctgc gaccttgtgg gcggagcccc cgagggcggt gagatcgaac
     1741 tcatagcctc cgacggctac aaggtcaaac tcaactacac caacatctac gaaccgctcg
     1801 gacgccaggg cgaggccata gtcgcctggt gggcggaggg gcggggctac gtccccgact
     1861 accgtgacgg cccgcgcctc ttcttcaatg cgcccgatgg catctttgga aacgacgaca
     1921 tgcggacatg cctggctgaa gactactggc actactactg ggacggcggc atccagtacc
     1981 cctcggctgc cggtcttgcc aacaagaaca tcgcaaccat cgcgatcaaa ccaggggcac
     2041 gggaggactg gacgcttcta ctgagaggcg ccatcaccga taccatcacc cgctcctact
     2101 tcgagtcggg caaagcctgc acgatgggcg gacacggtgc cacctggacg gatgatgagg
     2161 ggcatgtctg gtcggggatg ccgctctggt tcctatgcgg ctgggttgac gatggaaaca
     2221 agcacgatta cggcacaaac ccattccgtg acgatctcgc ggcggcgggc tacaacgtga
     2281 ccgtcatcga ttacggcgct gacggcacaa agggcaccga tgacgacttc tctgccacat
     2341 tcaacagctc gtttgttgcg cggaacaaca acatcatcgt cgccgacgag atcgacggcg
     2401 ccccgcttcc aaaggatggc aagacctggc cgctcaaact tgtcggctcc gccctgacct
     2461 ccaacaagca gagggtcggg agcatcgatg agatcgtgct caccggtgtg ccgatcgtcg
     2521 aggagccccc agaagccaac gccacgatcg accttgagga gggctggaac ttcgtctcca
     2581 cgccgaaacg gctcatggac ggctcagaca cattcgagac catctacggc aacgtcgata
     2641 ccgccggcca ctcgatcctg ctctacgacg ggctcacaca ggagtggacg gctgtaacct
     2701 caaatgagac attcaaaccg ctcgatggcg tctgggtcta tgcaaacgag acctgtacag
     2761 tccccctgac atttgctccg gccggacctg aaaccccgcc aacaaagaac ctgggcgagg
     2821 gctggaacgc gatcgggttc accgacaccg ttcccgagtc cgctgcaaac accctgctct
     2881 ctctgggcga tcactggaca accctgatcg ggtttgatgc aggaagccag gaatacgagg
     2941 tctcgatcgt caggggcgcc accggccggc acggtgagga acgcacgatg gagcctatgc
     3001 agggctactg ggtctacatg acaggagctg acatgcttgc tgcaataggt gcctgaggat
     3061 gaagtgtatg aggttgaaac aacacggtat cgtgctcctg ctcctcctcg ggcttgtcgc
     3121 cggggcagcg gcagcaacgg agggatctcc ccccgttctg ccgcatgagt tctacggtaa
     3181 cgtgatgata ggcggatccg cggcaccggc agggaccgag atcaccgcca cgatcgatgg
     3241 ggtggggtgc ggttctgtcg tgaccacgga ggcgggacgc tacggcagtc ccgacccgag
     3301 cctcgggaac cgcctgattg tgacggcagc cgcggaccaa gagggggcgc cgataacctt
     3361 tctggtcaac gggagggctg ccaaagagac cgcaaacttc acctccgatg cggtaacggt
     3421 cctcgacctg agcgttgccg cggaggccac accgaccacg ccaccgggcg gcagcaccgg
     3481 tggcggtgga ggtggaggta ggggcacctc cggttccccc ggatcagagg ttgtctatga
     3541 acccgaggcc cccaccgccc cggtgatctt cacaaaagag gccaccctga ccgcatcgac
     3601 cgctggcgtg gtgctcgaac cggtggtggt caggacagag gacgaggttg ctgcgctgac
     3661 gatacctgag ggcaccaccg cccttgatgc cggaggcaac cctctcacct ctgtcacatg
     3721 cacaaaggtc gaccccgcgg aagtcccgcc ggcgccaccc ggcgcgagtc tcgccatagc
     3781 cctttcatgc ggccctgacg gcgcaacctt cgacccnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnttgc gctctgcctg ctgcttgcgc tggcagcacc tgctgccatg
     3961 gcagcgacaa ccgaactcca cgtcgtcagg tatgcagcag acggagtgac catcttaaac
     4021 gagacgacgg tcgactaccg ctggcttgag tcaaacctcc ctgtccaggg ggacggtgtg
     4081 acacactact accaccaggg ccccgtattc gagggggata agtggaaccc cgaagaggat
     4141 acaaacgtcc ttgaaaagga tatgggcgcg gtgaagggga ccgacctcaa ggatatctgc
     4201 gaccttgtcg gcgggatgaa ggagggcgag acagtcagga tcaaggcatc cgacggactc
     4261 tcaagggttt ttccctaccg caacgtctac gaacccgaac cccgccaggg gccgatggtc
     4321 ataacgtggt accacgacga agacggatac gtccccgact actacagtgg gatgaggctt
     4381 gtcttctttg cggatacctc cacaaacccc tacggtgtcc acgctttcgg cgtatgggac
     4441 atgcacgagt gctttgacga ggaatactgg tacttctacg gcggtgagta tccaacaaca
     4501 accgggctct ccgtccagta cataagcgag atcctcatct acagcgagga agagcccacg
     4561 ggcggcatcc acgtgaactc aaccccctcc ggggcaacca tcatcctgga tggtgaagat
     4621 accgggtatg agacaccata caccctcacc ggcatcgagt cagggagtca ctccatcctg
     4681 gtcgagaagg ggggttacgt ccagcccgat gagaggtggg tcacggttgg ggcaaaccag
     4741 atcacggagg tcgagttcaa ccttaccccc atcacaggga agatcgccgt ctcctccgtc
     4801 ccgatcaacg ccagcatcat cctcgacggc gaggcgaccg ggctctacac cgacaccgtc
     4861 atggaggagg tgccggtcgg agagcacacg atcgaacttg tgctgccggg ttaccggaac
     4921 gccacccgga ctgttgtggt cgaggaggac gagtatagca ttctggatct cgtcctcatc
     4981 cccgtaaacg agagcgtgcc tggaaccctc ccggaggctt cgatgaatgc aacgccgggc
     5041 aacgccacac tgttttcgct gctcgggccg tttgagtttc atgggagggt tgacgttctc
     5101 ccggcaaaca tctccaccac ccccagcggg agcgtcgtga gttaccgcct ccctgacggg
     5161 gcgaaggggc gcggttacat ctacatcgat aacaggagcg ccggtgaacc ggatttcgaa
     5221 gggttcaacc ctgaccggcg atacaccgcc gcggctgccg cgacgtttgc cgcaaacctt
     5281 accggtgaga acagcaccac gtttacagcg cattgtccgc caggtgtcat ccccggcggt
     5341 ctcctcctga cagtggaggt ggacgagaac gcatcctcca tcatatgctg gataggcgag
     5401 gggctcctca tcccggatgg agagaggccc ctggtgtttg aaccggcgcc cgacctttcc
     5461 cggatctctg ccgccaggct gctgatcgtg ggtaccggta atatcaccgc caggttcaac
     5521 ggccacagca taaccgggat cccgcaaggc gaacccgcgg tcacggagta tgacgtgctg
     5581 gcctgcctga atgaatcatc aaacgaactc cgcctggaag gagaggacgc cgtgatcagg
     5641 aacgtcatcc tcagcctgac caccaccgac ggtatgccgg aaccctcgcc aacaccggta
     5701 gaggcccgga agtctcttat cgagaggcta ctcgggctcc tcgccgggat cttcgggttc
     5761 tcaaccgaag aaccggaaca gaataccacg agagatctcc ccgaggttga ggagaccgcc
     5821 gtccccgaag agacacgacc caccccggcc gtgacaccca cagtgaatcg ggccgtgaag
     5881 aaccactcgg gcggggttta catcacctca tatccgccgg ggatgacgat catcgtggac
     5941 aacaaaaaac tctcacgcca gacaccccgc gttatctacg gcctccgtga ggggctgcat
     6001 accatccagg tcgaggcgag cacaacgtat ccagggcagg aggagcccgg ttaccggttt
     6061 gagaccgtcc aggcctgggt ctacccggac gcgatcacac ccgtccatct cgatggcgtg
     6121 gctccctcaa gccgaaagac cgtccgggtc gagtcggagg cctacagggg tgagaggttc
     6181 accttaaacg ggatctttcg cgccggcacc atacctggcg agggggagat agagggcgga
     6241 gcggcatggg tcaccgtcct gcacgaggga cgctacctct cctcctccct gcccacgccg
     6301 gttgaagatg gcatgagatt cgtggtcgaa ccctggactg gtgagaccgt atccctcagt
     6361 gtcaggtcga acccccccgg cgcgcttgtc ttcatcgatg gtttccccac agatgagaga
     6421 accccctgcc gtatggatgg actctcgccc gggcggcacc ggattctggt ctcgatgccg
     6481 ggctatctcc ccgccgaaga ggtgatcacg ataccggaag gctcgaggaa cggcggcgtc
     6541 ataacctgca ccctccagga atacaccagc ggtggcctcc tggtcgagag caacgttcct
     6601 gacgccagga tataccttta cgggcgctac accggcgaga agaccccgca caccttcacc
     6661 gggatgagca tcggcacata cgaagtccgt gtggtctctg agaacgattc caggaccgtc
     6721 gaagatgtgc ttgtgaaagc gggggcgacg acccggtgcc tggtgaccct ggagcggtga
     6781 gtgagaaatg aaacgttact gcaccctact tgcaactgcg acggctgcgc tgctgctcat
     6841 tcaggcagcg tcagccggca tcgtgtgggg gccctatgtt accaacacca ccagcaactc
     6901 gacggtggtc tcctggaagg cgaccggcga taccggcgga tgggtggagt acgccgccgt
     6961 cgaaggcggc gagaccaacc gtataccctc atccgggacg gaggggggta tccagcacgc
     7021 ccacctcagc gacctctccc cggcaacaac ctaccgctac cgtgtggtca gcgggaacga
     7081 gacaacagga gactgcaggt tcaggacgtt cggcgacctg gagtttacgt gtatcatata
     7141 cggcgacacc cgcggccaga aaccactctt cacccaggcc gaccggcacc gccttgtggc
     7201 agagaggatc gctgaagagg aagagttcct ctttctactc cacaccgggg actttgtctg
     7261 cgacgactcc gagtgggacg agttcttctc tgtggtagga gaggcgctca ataagacaac
     7321 cctggttccg gtggcaggca accacgacgg caccgcagag gcgttctcca ggatctttgc
     7381 cgtccccaca gactattcct ttgatgccgg gagcctccac gtgaccgtcc tcgacagcaa
     7441 cgaccgcgcc tgggcggata tggcgaaccg gactgcctgg ctcaaggaag acctcagatc
     7501 ccctctgcca cgaaagatcg tggcgttcca ccacccgccg ttcagcgccg atcggaaacg
     7561 cccgggcggt gacctgggga tcagggccga gtgggctgag atcttctcag agaacggtgt
     7621 cgacgcggtc tttagcgcgc acacccatgc ttacgagcgc tacagcgtga acggcacaga
     7681 ctactttgtt atcggttgcg gtggcgcccc gttctacccg ctttcggatg agaagcctga
     7741 gggatatcga atggggtgcg aggagacgct cggctacctc cgggcgagag tcagccctga
     7801 atcgatcatc atagagatga taccggttgc aaaggttatt gacgatgagg ttgtgctcta
     7861 cccgcggggg tttgtcattg agacagtccg cctcccggcg gggtggtgga gcgtcttccc
     7921 ctcagttgca ggtgtaacaa gactgccaga attatggaga tgatggattt gcatgcagag
     7981 atcagacgga ttctgctcgc gctcctctgc gtgagcatac tggtgatcgg cgccgtatcg
     8041 gcggcgccaa cgaccgaact gcggatagta aagatcggtg ccgacggggt gaccgtctta
     8101 aacgagacaa cggtcgacta ccgctggatg gaagagaacc tgcccgtcct cggggacggt
     8161 gtgacccact actaccacca gggccccgtg tttgaggggg acaaatggga c
//
LOCUS       DAOSZX010000066         8028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7649_length_8028_cov_20.663866, whole genome shotgun sequence.
ACCESSION   DAOSZX010000066 DAOSZX010000000
VERSION     DAOSZX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_8028_cov_20.663866"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /gene="purL"
                     /locus_tag="PK196_06140"
     CDS             <1..1763
                     /gene="purL"
                     /locus_tag="PK196_06140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HON41033.1"
                     /translation="MGACPIALMDPLYFGPLDSEKNRYIFEHVVAGVGGYGNCIGVPV
                     VRGELVFESSYSGNPLVNVVCVGIVDPDRYITARVKKPGNHLVLIGSSTGRDGLGGAS
                     FASRDLAEDAEAADRPSVQIGDPYTEKLLIDAILAMAATGKVLSCRDLGAAGLAGASS
                     EMASTFGAVIHADRVHLRETGMTPREIMLAESQERMLVEVAPEDVTLIGSIAERYDLA
                     WSDVGEVIAEPRYIVRYLGETVADLPIDLLVGGVPPCSWEKKPYSAERPFSRPEAPVK
                     DLALSVLAHPDVARKDWVYEQYDKDVQVRSVSLVHDAAVLRLEDKALVLSCGCNPSQI
                     FLKPYDGAANAVIENAGNLACLGAEPLCIVDCLNFASPEHPEVAWQIEQCILGMGEMA
                     RKMGIPIVGGNVSLYNESDEFDTRIMPTPSIGMLGRGEVRRWTAPNEGDLIALVGRTL
                     PDFGGSVLDAVTGCGGPAPAMADPAVVAIVRGLVASGSVTAATDLSRGGLLAALAKVA
                     PRAEVALAGDPLEELFSETCGRFLLAVRDEAALAGVKHRIIGTVGGDTLTIRLEGESI
                     VIPPEELDAALSTTTRTMRY"
     assembly_gap    1818..1917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2044..3033)
                     /gene="ilvC"
                     /locus_tag="PK196_06145"
     CDS             complement(2044..3033)
                     /gene="ilvC"
                     /locus_tag="PK196_06145"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HON41034.1"
                     /translation="MVQKYYESDADPRILDGRTIAVIGYGSQGRGQALNLRDSGCRVI
                     IGLRPGNSWQRASEDGFEVYPVAEAARRADVVQILLPDENQAAVYRAEISPNLKENTC
                     LMFSHGFNIHYGQIVPPPTVDVVMVAPKGPGHMVRRMYEEGKGVPALIAVQQDATGDA
                     HAIALAYARGIGATRAVVLETTFAEETETDLFGEQAVLCGGVTSLIKAGFETLVNAGY
                     APEMAYLEVLHEMKLIVDLIYEGGFTKMRDSISNTAQYGDLTRGPRVIGPEAYAAMEE
                     ILEEIQSGEFAREWMLENLVNRPVFNALTKADEEHLIEQVGAEIRRFMPQFQK"
     gene            3168..4109
                     /gene="mptA"
                     /locus_tag="PK196_06150"
     CDS             3168..4109
                     /gene="mptA"
                     /locus_tag="PK196_06150"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HON41035.1"
                     /translation="MELPDVQSSLPEVRISLTRVGVKNVMKLVEVARSGKRPVIFISN
                     FDVFVDLPGSLKGANLSRNFEVIDDVLQQAIDGEVKGIEEVCSAVARKLLDKHEYAER
                     TEVRMRSQFMVRRETPVSETTCHEVVNVHARAIAQRNDGNPIIRKSIGAEVVGMTACP
                     CAQNIMKDHALHVLEQLGVEEDKIARFFEEVPMATHNQRGRGFLCIEIDDDQQISLEK
                     IIKILKDSMSARIYELLKRGDESYVVMEAHKNPRFVEDCVREMARKVVAQFGDLPGDT
                     VVTIKQTNEESIHQHDAYAERKATIAELVSELERGAL"
     gene            4336..5703
                     /gene="frhA"
                     /locus_tag="PK196_06155"
     CDS             4336..5703
                     /gene="frhA"
                     /locus_tag="PK196_06155"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HON41036.1"
                     /translation="MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVE
                     KLAIGKTMEQVPKIASRVCGICPIAHTLAGVEAMEASIGCEIPEDAKLLRYILQCANR
                     MHSHAIHNILSLPDMYIPGTDTKINPFTKEEPVRSVALRIQRLREIGQTIGEIVGGEA
                     IHPSNPRVGGMYTNITPRAKAKIYDLAKEARVLAQQQMEFMIAVFRNYQKRDWAEVGG
                     VEVPIPKTLGYHNQGYMATAPVYGSSSLDENPTWFPERFTEVRPWDWYMGEVEITEAD
                     PNYPIGGTTPVGNKAWPQMEACTGVPLYDGQPVEVGPRARQAIFKNYDEKGTIGLQIA
                     RQMEFPETAYGIIDAVDALNTSGPVLADEIPQGDGSLGWAANEAPRGTDVHLARVKDG
                     RVQYYSMLVPTTWNFPTCSRALEGAPWRLAEVVMRGYDPCVSCATHMIVIDEDKRLVA
                     QKLIQ"
     gene            5714..6274
                     /gene="frhD"
                     /locus_tag="PK196_06160"
     CDS             5714..6274
                     /gene="frhD"
                     /locus_tag="PK196_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HON41037.1"
                     /translation="MLFREIVIAGCGNPLFGDDGFGPAVVEELKKLQLPDNVKVIDAG
                     LGAPHFLFTLIEDAEVPVKKLVIIDIADFGGNPGDVTKLRPEDLPPGAYRDAHSWDLS
                     EPLQRLKDAIDITIIGCQPKRVASHEFELGLTEEVEGAIPKTVRIVLEEIGVEYGATI
                     NHQGTHLWASPGEAGGDAGENPGEQT"
     gene            6351..7112
                     /gene="frhG"
                     /locus_tag="PK196_06165"
     CDS             6351..7112
                     /gene="frhG"
                     /locus_tag="PK196_06165"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HON41038.1"
                     /translation="MAEKITIGELHLSGCTGCLVTLADNYAGLFKLLDDYADLVYALT
                     LVDVRHVPEMDVCLVEGSCCLDDKLSVEELKEARAKSKVLVAYGGCAAYGNITRFCRG
                     GQWNQPGHESYVPISEVVDVDLYIPSCPPCPQEVRNVAVMAYLLLRGNAEQKKLATAY
                     LTPLMQLAQRGDTACGCDLMYEVINQGLCMGCGTCAGTCPVRAITMEYGKPNVNRDQC
                     IKCGACYAQCPRSWLNFDVINNYEGIIGAIKGAMQ"
     gene            7123..8010
                     /gene="frhB"
                     /locus_tag="PK196_06170"
     CDS             7123..8010
                     /gene="frhB"
                     /locus_tag="PK196_06170"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HON41039.1"
                     /translation="MDVLGNYKSVVSARSTDKEILKHSQDGGIITTLFAYALEEGIID
                     GAIVAGPGSEPWKPEPVVATTKAELLAAAGTRYNISPNLCLIKEVTRSYGLDKVGIVG
                     TPCQMQAVRKAQLYPIGLRDVDDKIALALGIFCMENFSYQGLEAMVEDHCNQKMESVK
                     KMDIGKGKFTVYTERGAVSQMPLKLIHKYVQPGCHVCLDYVANLADISSGSVGSPDGW
                     STVFVRSTIGNGVWDGAIAAGLFETKPIDKVKPGLDMVTKLATEKITKNQKNVDARKT
                     VGLKADGTPKGLRNPYEGP"
BASE COUNT         1689 a         2289 c         2412 g         1538 t
ORIGIN      
        1 cgatgggtgc ctgccccata gccctgatgg acccgctcta cttcgggccg ctcgactccg
       61 agaagaatcg ctacatcttc gagcacgtcg ttgccggtgt tgggggttac ggcaactgta
      121 taggggtgcc ggtagttcgg ggggaactcg tcttcgagtc ttcctactca ggaaacccgc
      181 tcgtgaacgt cgtctgtgtc gggatagtgg atccagaccg ctacatcacc gccagggtga
      241 agaaaccggg caaccatctc gtcctgatcg ggtcatcgac cggccgtgac gggcttggag
      301 gggcatcgtt tgcatcccgc gacctcgctg aggacgcaga ggcggccgac cgtccgagcg
      361 ttcagatcgg tgacccctac accgagaaac tcctcatcga tgcaatcctc gctatggccg
      421 cgaccgggaa ggtcctctcc tgccgcgacc tgggggcggc gggacttgcc ggggcttcga
      481 gcgagatggc aagcacattc ggtgcggtca tccacgctga ccgtgtccac ctccgggaga
      541 ccgggatgac accgcgggag ataatgcttg ccgagtcgca ggagcgtatg ctcgtagagg
      601 tggccccgga ggatgtcacc ctgataggat caatcgccga gaggtatgac ctcgcctgga
      661 gcgacgtcgg cgaggtgatc gctgaacccc gctacatcgt gagatacctg ggtgagacgg
      721 tcgccgatct cccgatcgac ctgctcgtcg gcggcgtccc gccctgctcc tgggagaaga
      781 aaccctactc ggcggagaga ccgttctcca gaccggaggc gccggtaaaa gacctggcac
      841 tttccgtgct tgcgcatcct gacgtggcgc gcaaggactg ggtctacgaa cagtatgata
      901 aagatgtcca ggtcaggtcg gtctcgctcg tccacgacgc tgccgtcctc cgcctcgagg
      961 ataaggcgct cgtcctatca tgcggctgca acccctccca gatcttctta aaaccttacg
     1021 acggggcggc aaacgccgtc atagagaacg caggcaacct cgcctgcctt ggcgctgaac
     1081 cgctatgcat cgtcgactgc ctcaattttg caagcccgga gcacccggag gttgcgtggc
     1141 agatcgagca gtgcatcctc ggcatggggg agatggcacg gaagatgggg atccccatcg
     1201 ttggcggtaa cgtctcgctc tacaacgaga gcgacgagtt cgataccagg atcatgccca
     1261 caccctcgat cgggatgctc gggcgcggag aggtcaggag atggacagcc ccgaatgaag
     1321 gcgatctcat cgccctggtg ggccggacgc tgcctgattt cgggggttcc gtccttgacg
     1381 cggtcacggg atgcggcggt ccggcgcccg caatggccga tcctgcagtt gttgcaatcg
     1441 tccgtggcct ggtcgcctcc ggttcggtca cggcggcaac cgacctctca aggggcgggt
     1501 tgctggcggc gcttgcaaag gttgccccgc gtgcggaagt tgcactcgca ggtgatcctc
     1561 ttgaggaact cttctccgag acctgtggcc ggttccttct cgccgtcagg gatgaagcgg
     1621 cgctcgccgg tgtaaaacac cggatcatag gcacggtcgg cggcgatacc ctcaccatcc
     1681 gactggaggg ggagagcatc gtaatcccac ccgaagaact tgatgccgca ctctccacga
     1741 ccacccggac gatgcgttac tgagcaatca atccttcttt tctgacctgc ggacgagatc
     1801 ggccagggat tctatcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     1921 gcagcgagcc gttccgcaac agatcgggtg ttcccggtct cagagtggta tatgatgcag
     1981 agagacatac ccgagagtgg cgcgggccgg gtataaaccg gtatcggcca ctaaaaaatg
     2041 gtgttacttc tggaactgtg gcatgaacct gcggatctcc gcacccacct gctcgatcag
     2101 gtgctcctcg tctgcttttg tcagcgcgtt gaagacgggg cggttcacca ggttctcaag
     2161 catccactcc cgcgcgaact caccgctctg gatctcttcc aggatctcct ccatcgcggc
     2221 gtaggcctcc ggcccgatta cacgtgggcc gcgggtcagg tcgccgtact gggcggtgtt
     2281 gctgatcgag tcgcgcatct ttgtgaaccc tccctcatag atcaggtcga cgatgagctt
     2341 catctcgtgc aggacctcga ggtaggccat ctcgggggcg tagccagcgt tcaccagcgt
     2401 ctcaaatcca gcttttatga gggaggtcac accgccgcag aggaccgcct gctcgccgaa
     2461 gaggtcggtc tcggtctcct cggcaaacgt cgtctcgagc acgaccgcgc gtgtcgcgcc
     2521 gattccacgg gcataggcaa gggcgatggc atgcgcatcc ccggtggcgt cctgctgaac
     2581 agcgatgagg gccggcacgc cctttccctc ctcgtacatc cggcggacca tgtggccggg
     2641 gcctttcggg gcgaccatga ccacgtcaac tgttggcggc gggacgatct ggccgtaatg
     2701 aatgttgaaa ccgtgtgaga acatcagaca ggtgttctct ttgaggtttg ggctgatctc
     2761 ggcgcggtat acggccgcct ggttctcgtc ggggaggagg atctggacga catcggcgcg
     2821 cctggccgct tcagcgacag ggtagacctc aaaaccatcc tcagacgctc tctgccagct
     2881 gttccctggc cgcaggccga tgatgacccg gcacccggag tcacgcaggt tcagcgcctg
     2941 tccccggccc tgggagccgt atccaatgac ggcgattgtc ctgccatcca gtatgcgtgg
     3001 gtccgcatcg gattcatagt atttttgcac catagtttct cacttcccct ttggagagca
     3061 aacctgataa atattctatc ggtgaacctc tctggtgcac tgggggttat tttaagattg
     3121 atggggaaga aactttattc ttcataacag gcgattgagg atttataatg gaactgccag
     3181 atgtccagtc cagcctgccg gaggttcgga tcagcctgac ccgggtgggc gtgaagaacg
     3241 tcatgaaact tgttgaggtt gcccggtcgg gtaagcggcc ggtcatcttc atctccaact
     3301 ttgacgtctt cgttgacctg ccagggagcc tcaagggtgc gaacctctca cggaacttcg
     3361 aggtgatcga tgacgtgctg cagcaggcca tcgacggtga ggtgaagggg attgaggaag
     3421 tctgcagcgc ggtggccagg aaacttcttg ataaacatga gtacgcggaa cggacggaag
     3481 tccggatgcg gagccagttc atggtacggc gtgagacgcc ggtcagcgag acaacctgcc
     3541 acgaggtcgt gaacgtccac gcgcgagcga tagcgcagag gaatgatgga aacccgatca
     3601 tcagaaagag cataggtgcc gaggtggtcg ggatgaccgc ctgcccctgt gctcagaata
     3661 tcatgaagga ccatgccctc cacgtcctcg agcaacttgg tgttgaggag gataagatcg
     3721 ccaggttctt tgaagaggtg ccgatggcca cccacaacca gcgtgggcgg ggtttcctct
     3781 gcatagagat cgacgacgac cagcagatca gcctcgagaa gatcatcaag atcctgaagg
     3841 actccatgag tgcacgtatc tatgagctcc tcaagcgggg cgacgagagt tatgtggtga
     3901 tggaggctca caagaacccg aggtttgttg aggactgcgt ccgtgagatg gcccgtaaag
     3961 tggttgcgca gttcggggat ctccccggcg acaccgtggt caccattaaa cagacaaacg
     4021 aggagagcat ccaccagcac gatgcctacg cggagcggaa agcaacgata gccgaactgg
     4081 tctcggagct ggaaagaggc gcactatagg ctccagcctc aattccatca ttttttatac
     4141 ttgcctgccc tcccggtgct tggtgttact gttgctctat tacgtaacac tcagaaacat
     4201 cccaaagcga aagatataaa ctctgtttcc ttcataatcc atgttgacgt actcgtgtac
     4261 gttttcggta aagaattgtg tggtaacacc agtatcgtgt tcagaacacc acattatcgg
     4321 aatgaggcga tatatttgtc gaaagttgta gagatttccc caacaacgag gcatgaaggc
     4381 cactcaaagc tcgtcctgaa ggtcaatgat gaaggaatca tcgagcgtgg agactggctc
     4441 agcatcaccc cggtgagggg agttgagaaa cttgccatag ggaagacgat ggagcaggtt
     4501 ccaaagatag catcacgcgt ctgcggtatc tgccccatcg cccacaccct ggcgggtgtt
     4561 gaggcgatgg aagcttccat cgggtgcgag atccccgagg acgcaaaact cctgcgctac
     4621 atcctgcagt gtgccaacag gatgcacagc cacgccatcc acaacatcct gtccctcccg
     4681 gatatgtaca tccccggaac agacacgaag atcaacccgt tcacaaagga agagcctgta
     4741 aggagcgtcg ctctccggat ccagcggctc cgtgagatcg gccagaccat cggcgagata
     4801 gtcggcgggg aggcgatcca cccgagcaac ccccgcgtcg gcggtatgta cacgaacata
     4861 actccgcggg cgaaagcgaa gatctacgac ctcgcgaagg aggcccgtgt gctggctcag
     4921 caacagatgg agttcatgat tgcggtcttc cggaactacc agaagcgtga ctgggctgaa
     4981 gtcggcggcg tcgaggtccc gatcccgaag actcttggct accacaacca gggctacatg
     5041 gccactgcac cggtctacgg cagttcaagc cttgatgaga accctacctg gttccccgag
     5101 cggttcaccg aggtccgccc ctgggactgg tacatgggtg aggttgagat caccgaggcc
     5161 gacccgaact acccgatcgg cggcaccacc cccgtaggga ataaggcctg gccccagatg
     5221 gaggcctgca cgggtgtccc gctctacgac ggccagcctg tagaggtcgg acctcgcgca
     5281 cgccaggcga tcttcaagaa ctacgacgag aagggcacca tcggcctgca gatcgcgcgg
     5341 cagatggagt tccccgagac cgcctatggc atcatcgacg ccgtggacgc gctcaacacc
     5401 tccgggccgg tgctcgccga cgagatcccg cagggcgacg gatccctcgg ctgggccgca
     5461 aacgaggctc cccgtggtac tgacgtccac cttgcccggg tcaaggatgg ccgggtgcag
     5521 tactactcga tgctggttcc gaccacctgg aacttcccga cctgcagccg tgcccttgag
     5581 ggtgcaccct ggcggcttgc ggaagtcgtc atgcgtggat acgacccctg cgtctcctgc
     5641 gcgacgcaca tgatcgtgat cgacgaagac aagaggttag tggcccagaa actcattcag
     5701 tgagcgtaca cgcatgctat tccgtgagat cgtgattgca ggatgcggca accctctctt
     5761 tggagatgac ggattcggcc ctgctgtagt tgaggaactc aagaaactgc aactgcccga
     5821 caacgtcaag gtgatcgatg ccggccttgg cgcccctcat ttcctcttta cgctgattga
     5881 ggatgcggag gtgccggtga agaagctcgt catcatcgat atcgccgatt tcggcggtaa
     5941 tcctggtgat gtgacgaaac tccggcccga agacctgcca ccgggcgcct accgtgatgc
     6001 ccattcgtgg gatctctctg agccgctgca gcggttgaag gatgccatcg atatcacgat
     6061 catcggatgc cagccaaagc gtgtcgcgag ccatgagttt gaactggggc tcactgagga
     6121 ggttgaaggg gccattccca aaacagtacg gatcgtactg gaagagattg gggtagaata
     6181 tggggctact atcaaccatc aaggaacgca tctttgggcg tcgccgggag aagccggagg
     6241 agatgccggg gagaaccccg gagagcaaac ctgaggcgaa gcccccggtg aagccctctg
     6301 atgtgattgt ggaagaaaag gtggaagttg tacaaaagga ggaaaagcct gtggcagaaa
     6361 agattacgat aggtgaatta catctgagcg gatgtacggg atgcctcgtg acgcttgcgg
     6421 acaattacgc gggtctcttc aagctgctcg atgattacgc ggatctggta tacgcactca
     6481 ccctggtcga cgtgcgccat gtccccgaga tggatgtgtg cctggtcgag ggttcgtgct
     6541 gcctggatga taaactctcg gtagaagaac tgaaagaagc aagagcaaag tcgaaagtac
     6601 tcgttgccta cggcggttgc gcagcctacg gaaacatcac ccggttctgc cgtggcggac
     6661 agtggaacca gcccggccac gagtcgtatg tgccgatcag cgaggtcgtc gatgttgacc
     6721 tctacatccc gtcctgtccg ccgtgcccgc aggaggtcag gaacgttgcc gtcatggcct
     6781 acctgctgct tcggggcaac gccgaacaga agaaactcgc gaccgcatac ctgaccccgc
     6841 tgatgcagct tgcgcagcgc ggtgacacgg cctgcggctg cgacctgatg tatgaagtca
     6901 tcaaccaggg tctctgcatg ggatgcggaa cctgtgccgg cacctgccct gtccgtgcaa
     6961 tcaccatgga gtacggcaag ccgaacgtga accgtgacca gtgcatcaag tgcggcgcct
     7021 gctatgccca gtgcccgcgc agctggctta acttcgacgt cataaacaac tacgagggca
     7081 tcatcggtgc catcaaggga gccatgcagt gaggtgatga agatggacgt actcggtaac
     7141 tacaagtcag tggtctcggc gcggtcgacc gacaaggaga tcctgaagca ttcccaggac
     7201 ggcggaatca taaccacgct ctttgcctac gcgctcgaag agggcatcat cgatggtgcc
     7261 atcgttgcgg gccctggcag cgagccctgg aagccggagc cggttgtcgc gaccacgaag
     7321 gccgaacttc ttgccgccgc cggaacgcgc tacaacatca gcccgaacct ctgcctgatc
     7381 aaggaggtga cccggagtta cggccttgat aaagtcggca ttgtgggtac accctgccag
     7441 atgcaggccg tccggaaggc tcagctctat ccgatcggtc tgcgtgatgt cgacgacaag
     7501 atcgctcttg cgctgggcat cttctgcatg gagaacttct cctaccaggg gctcgaggcg
     7561 atggtcgagg accactgcaa ccagaagatg gagtccgtca agaagatgga catcggcaag
     7621 ggcaagttca ccgtctacac cgaacgcggt gcggtcagcc agatgccgct caaactgatc
     7681 cacaagtacg tgcagcctgg ctgccacgtc tgcctggatt acgtcgcgaa cctggctgat
     7741 atctcaagcg gctccgttgg cagccctgac ggctggagca cggtcttcgt gcgcagcacc
     7801 attggcaacg gcgtctggga cggcgctatc gcggcaggtc tctttgagac aaagccgatc
     7861 gacaaggtca agccaggcct tgacatggtg acgaaacttg ccaccgagaa gatcacaaag
     7921 aaccagaaga acgtggatgc acgcaagacc gtgggcctga aggcggatgg aaccccgaag
     7981 ggtctccgga acccctatga ggggccctga aaaacactct tttttctt
//
LOCUS       DAOSZX010000067         8019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7669_length_8019_cov_22.876570, whole genome shotgun sequence.
ACCESSION   DAOSZX010000067 DAOSZX010000000
VERSION     DAOSZX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7669_length_8019_cov_22.876570"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..442
                     /locus_tag="PK196_06175"
     CDS             5..442
                     /locus_tag="PK196_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41040.1"
                     /translation="MVALLLVFSFSAGCTGSEESNGPTPTETAQATAAPTETPPSLIP
                     EPTQTLPPGKEVTFEVSEGLTPTITDDLRIVFSGGKGQDFVKSIDVVVIKSTGEVVTG
                     SMKPIKGEELIISDAKGENRVQITVSLVTGGSYKVVDKIVEVK"
     gene            complement(500..1180)
                     /locus_tag="PK196_06180"
     CDS             complement(500..1180)
                     /locus_tag="PK196_06180"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HON41041.1"
                     /translation="MRPFVFVNVAMSADGKISTAERRQVRISGGDDYSRVDRIKAESD
                     AIMVGIGTVLSDNPSLTVKSPDLRAWRREAGKDEDPIRVVVDSRGRTPTDADILHKGA
                     GKRIIAVSRAASPEALEILRKHATVIITGDETVDLSALLEELYRLGVRRLMVEGGGTL
                     IWALMERGLVDELQTFIGSIIIGGKDAPTPVDGTGFFREEDFPRLALIEAGRLDEGLL
                     IRWRVKRA"
     gene            1506..3197
                     /locus_tag="PK196_06185"
     CDS             1506..3197
                     /locus_tag="PK196_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HON41042.1"
                     /translation="MLTAVAPVNLSTPCTLVYCVPILLAALWFPLQALHVTVILVTGF
                     VLGQTSLSLLGYRVAPLLVGLHALLFFWVFGAVTLFSQDSYLTASRCRKIIENSRDAK
                     FLCEPGSLRVVCASKLFAAMLGYSPTEVVGISLEKLWPDESDRASFIQEMNKEGYIVN
                     IETIFYTKDGESRRVLLSGRPLAGEDLFECSVVDCGRFRDENGDLLRSDSRLNEFVRQ
                     TNDMFFMQDTSGRILHFFWARAPEYGLDPDKLAGRDLGAILPEDLARQHMAQVRKVID
                     ERKDVRYSLDLMIGGLRHTFSVSLCPYTAADGVLRGVIGSARDVTESRRQRLACRQLS
                     WEVDRWKHLTTTISHEMRTPLQPLIGYLQLVLDDPEYYGITPETESILKVCLDCAIQE
                     QAVVERAVELSVFATDYIDLNIQDIALRDLVDSVIASGGYEREALIRNEIPDDVHMPG
                     DARRLAVALESLISNAVRYNDPPKEVWIRYRESPENHYIMVCDNGRGIPKEKIRSIFD
                     PFYIRDACDQDKKGGGVGLSLSIANRYVRLHGGEITVASQVGEGSTFTIRIPKEV"
     gene            complement(3261..3917)
                     /locus_tag="PK196_06190"
     CDS             complement(3261..3917)
                     /locus_tag="PK196_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HON41043.1"
                     /translation="MRIKFCGTASLADLRCAVAAECDAVGFIMGVIHRSSDVVTPAEA
                     ARMIRQLPPFIEPVAVTHLQETQALIDLVRESHCTTLQVQNDIDPSDLEVIRQALPCV
                     KIVKAVHVMDRSSIAAARRYEPYADAILLDTRTEERIGGTGIPHDWNISAEIVANSTV
                     PVILAGGLTPENVRDAIQKVRPYAVDVHTGVKKDGARNLEKTLAFARAARTAKGKTAV
                     "
     gene            complement(3960..4033)
                     /locus_tag="PK196_06195"
     tRNA            complement(3960..4033)
                     /locus_tag="PK196_06195"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3997..3999),aa:Phe,seq:gaa)
     gene            4146..5123
                     /locus_tag="PK196_06200"
     CDS             4146..5123
                     /locus_tag="PK196_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HON41044.1"
                     /translation="MDGIDRKPEAHPGNLMQALSSRTESVVHLTHNDLDAVGSDAIHR
                     RVLGDDVFTVWSSVGRFLSLLDAVAASPGRGDLLSISDIGYQDGVEQRLARARSNGWR
                     IEWRDHHRWTKDEIRTVDKRTDILHIDVATCATGIVARDLAPGDPVAEEIARVVCDYD
                     LWKHLDPRSKMLGQVVMRKGLRDYVRDNFVRGIILDERIENEYAEILREMEHDIRRSI
                     RHATVIQGGRYRIVFAPLYGYPSETAHAIRQELGSDIEVIVSSNGRISIRSVPPISHL
                     VARQFSGGGHPHAAGGTFTFSLLDRFLFWLIKRNRHYRDLAAVAESIEE"
     gene            complement(5110..5898)
                     /locus_tag="PK196_06205"
     CDS             complement(5110..5898)
                     /locus_tag="PK196_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41045.1"
                     /translation="MARQIRIPVRSFGSEVGRPGVPELAEWLKGKTGTEVDLTTYRLE
                     RSLDAQDGVSIPAAGGLFYAERLREVFTSMKDGVLIDEPGVEPAAVTADALFVRERCK
                     DARIALPAPHIMGFRDDYIGDREEFSETISDLYAGLMRDMRDQGLAGHVLIADTPDLI
                     ELERLAGRKVLFFPRNPARFDLELLLEYQDSLILPATDLDRLPDLMERFRLRKLILLN
                     PEGGEVVAAAGIVDADSLEVGGYCADTACPDYWKRLLDRAFIPR"
     gene            5941..7935
                     /gene="metG"
                     /locus_tag="PK196_06210"
     CDS             5941..7935
                     /gene="metG"
                     /locus_tag="PK196_06210"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HON41046.1"
                     /translation="MSGKPLLVTCGLPYTNGPCHIGHLRTYVPADFYVRYMRRSGEEV
                     AFICGSDNHGTPIVISAEQEGSTPRALSERYHEHFYDTFRRMDVVFDHFGMTDDSMNH
                     ETTRSIVQRLIDNGYVYEKTVSQSYCPQCERFLPDRYVEGVCPHCGAIARGDECDQGC
                     GQHLEPGELRDAVCKICGSKAEYREQEHYFFRLSAFRDFLLDYLPTLRGTATARNYAL
                     GWVKEGLRDWCITRTLDWGVKFPGRDDLVVYVWVDAPIGYISFTKEWANAAGVDWKDY
                     WCGDTTRVTHFIGGDIIYHHCIFWPALLKGAGYGLPHAVVASGMVTIDGRKFSKSRGY
                     VVWTNDDYLDQGLPADYLRYYLLSYTSHTKELDFSWKVYAERVNNEIVGTLGNFIYRT
                     MYFAEKEFSGVPDVAPQPGIIEEIERSLAAIDARMRDYDFKGAVDGMMSLASFGNAYI
                     QNNAPWKLIKEDQAAAKQVIADCLQMVKALTVVFQPLMPGSMQRAWTMLGYGDDLAAH
                     PIAEATAPIGARALGKPVPLFKKIEKEQVEALEKVLEARVAKASEAAGAKAPTVSIDE
                     FSKLDIRIARVVAAEPIDGSKKLYKLILDVGGEKRQVVSGIAQFYTPKDLVGREVAVV
                     VNLAPAKIFGVESRGMILAAGDEASLLVPLRPVSPGTKVR"
BASE COUNT         1666 a         2308 c         2316 g         1729 t
ORIGIN      
        1 cctactggtt gcgctcctgc tcgtcttctc attcagcgca ggatgcacag gctctgaaga
       61 gtcaaacggc ccgaccccga ctgaaaccgc ccaggcaaca gccgcgccaa cggaaacacc
      121 gccatcattg attcctgaac cgacacagac cctgccgccc ggaaaagagg ttacattcga
      181 ggtctcggaa ggcctgaccc caacgataac agatgacctg cggatcgtct tcagcggcgg
      241 taaaggccag gattttgtga agagcatcga cgttgtcgtg atcaagtcga ccggagaggt
      301 tgtcaccggt tcgatgaagc caatcaaggg cgaagaactc atcatcagtg atgctaaagg
      361 tgagaatcgc gtccagataa ccgtatcact tgtcacagga ggctcctaca aggtcgttga
      421 caagatcgta gaagtcaaat aatcccacct ctctcttttt gtcgccatcg gcgtgcattc
      481 atccactcga gccctatcat cacgctctct tcaccctcca ccttatcagc agaccctcat
      541 ccagccggcc ggcttcgatg agcgcgagcc gcgggaagtc ctcctcgcgg aaaaaaccgg
      601 tgccgtcgac aggggtgggg gcatcttttc cgccgattat gatgctgccg atgaaggtct
      661 gaagttcgtc gaccagcccc cgctccataa gcgcccagat taaagtccct cctccttcaa
      721 ccatcaggcg ccggacaccc agccggtaga gttcttcgag cagggctgaa agatcgactg
      781 tctcatcgcc ggttatgatg acggtggcgt gcttgcggag aatctccagc gcttctggcg
      841 atgctgcccg cgagacggct atgatccgct ttcccgcccc cttgtgcagg atgtctgcat
      901 ccgtgggtgt cctccctctg ctgtccacaa cgacacggat cgggtcttca tcctttccgg
      961 cctctctccg ccatgccctg agatcgggcg acttgaccgt gagcgagggg ttgtcggaga
     1021 gcaccgtgcc gatcccgacc atgatggcgt cgctctctgc cttgatccgg tcgacccgcg
     1081 agtagtcatc accgcctgag atcctcacct gccggcgctc agcggttgag atcttcccat
     1141 cagcgctcat ggcaacattt acaaagacga aaggacgcat atttctgggt tgaacgacga
     1201 ccttcataat gctaccctcg gttgcggcag gaatcatcat cgggcaggct gcaccacctc
     1261 tccgtgagat ctggggaggt tcatgagaaa atccggaaat acccgggtat accggattat
     1321 ttttgtaaac atcattttaa aatcgtgtga cgttttcgga gcctataaat atagataatg
     1381 gtgagatctc tctgttggac atgcccgaga accggcatct cttgagttgc ctatgccagg
     1441 atcgcttcac cgcgacagat ctcgtcaaaa tgatcgttct atcatcattc gcaggggtat
     1501 cgcttatgct cacggcggtt gcgcctgtga atctctccac accctgcacc ctggtctact
     1561 gcgtcccgat cctccttgcc gctctctggt tccctttgca ggcgctccat gtgaccgtga
     1621 tcctcgtcac cggtttcgtg ctcggccaga ccagcctctc cctccttgga tacagggtcg
     1681 cccctcttct tgtggggtta cacgcactcc tctttttctg ggtcttcggg gccgtaactc
     1741 ttttctcaca ggactcctat ctgactgcgt cccgctgcag gaagatcatc gagaacagcc
     1801 gcgatgcaaa attcctctgc gaacccgggt cgcttcgcgt tgtctgtgcc agtaagctgt
     1861 ttgccgccat gctgggctac tcgcccacgg aagttgttgg catctccctg gagaaacttt
     1921 ggccggatga gagcgataga gcttcgttca tccaggagat gaacaaggag ggctatatcg
     1981 tgaatatcga gacgatcttt tacacgaaag acggagagtc gcggcgggtt ctcctctcag
     2041 gccggccact ggccggcgag gatctcttcg agtgcagtgt tgtggactgc gggaggttca
     2101 gggatgagaa cggcgatctt ctgcggtcag acagccgcct gaatgagttt gtccgtcaga
     2161 caaacgatat gtttttcatg caggacacct cgggccgcat cctccacttc ttctgggccc
     2221 gtgccccgga gtatggtctt gatcccgaca aactggccgg acgggatctg ggggcaatcc
     2281 tgccggagga ccttgcccgt caacacatgg cgcaggtcag gaaggtgatc gatgagcgaa
     2341 aagacgtccg ttacagcctc gacctgatga tagggggtct ccggcacacc ttttccgtca
     2401 gtctatgccc ctataccgcg gctgacggcg ttctcagggg tgtcattgga tcggcacgcg
     2461 atgtcaccga gtcgcgccgg cagaggcttg cctgcaggca actctcatgg gaggttgacc
     2521 gctggaaaca cctcaccacc accatctctc atgagatgcg cacaccgctg cagccgctca
     2581 tcgggtatct ccagctggtc ctggatgatc cggagtatta cggcataact ccggagaccg
     2641 aatccatcct caaggtctgc cttgactgtg caatccagga gcaggcggtt gtggagaggg
     2701 cggtcgagtt gagcgtcttt gcgaccgatt atatcgacct gaacatccag gatatagcgc
     2761 ttcgcgacct ggtcgactcc gttatagcca gtggaggcta tgagcgtgaa gcattgattc
     2821 gaaacgagat acccgatgac gttcacatgc cgggcgatgc caggaggctt gccgttgcgc
     2881 tggagagcct gatatcaaac gcggtcaggt ataacgaccc tccgaaggag gtgtggatca
     2941 ggtacaggga gtcgcctgaa aatcattaca tcatggtctg cgataacggc agaggcatcc
     3001 cgaaagagaa gatcaggtcg atcttcgatc cgttctacat tagagatgca tgcgatcagg
     3061 ataaaaaagg cggcggggtg ggcctgagcc tctcgatcgc taacaggtat gtccggttgc
     3121 atggagggga gataacggtg gcaagccagg ttggcgaggg gagcaccttc acgatcagga
     3181 taccgaaaga ggtatgagag cctgaaggga tcatataatc atcaggcggg atctggcctt
     3241 atttctgcgc tgcctaaagt ttatacggcg gtcttaccct tggcggttct tgccgcccgg
     3301 gcaaacgcaa gcgtcttttc caggttgcgg gcaccgtctt tcttgacgcc ggtatgcaca
     3361 tcgacagcgt aggggcgaac cttctgtatc gcgtctctga cattctccgg ggtgagtcct
     3421 cctgcaagta tcacgggaac cgttgagttt gccactatct ctgcacttat attccagtca
     3481 tgagggatcc ccgtcccccc gatcctctct tccgtcctgg tatcaaggag gatggcatcg
     3541 gcatagggct cgtagcgtct tgccgccgca atagatgatc tatccatcac atggacggcc
     3601 ttcactatct ttacgcaggg gagagcctgg cggatgacct ccaggtcgga tggatcgatg
     3661 tcattctgga cctgcagcgt cgtgcagtgc gattctctca ccaggtcgat cagtgcctgc
     3721 gtttcctgga ggtgggtgac cgcgaccggt tcgatgaacg gtgggagttg acgtatcatc
     3781 cttgcggctt ctgccggggt tacaacatcg ctgctccggt ggatcacacc catgatgaat
     3841 cccacggcat cgcattccgc agcgactgca caccgcagat cagcaaggct ggcggttcca
     3901 cagaatttaa tgcgcatcag aagatctgat gcagcaagac cagaaaaagg catcgggagt
     3961 gcccccgccc ggatttgaac cggggacaac cagatcttca gtctggcgct ctcccagtct
     4021 gagctacagg ggcccctgcc taggagtgtt ggcagtagtt cttaataaat tctgtgttgc
     4081 ccccccggga gtggacggga tagcaaaccg cctgtttatc tcatcataga accgatgttt
     4141 gaatgatgga cggaatcgat cgaaaacctg aagcccatcc agggaacttg atgcaggccc
     4201 tctcctcaag gacggaaagc gtcgtgcacc tgacgcacaa cgacctcgac gccgtcggga
     4261 gcgacgccat tcaccgtaga gtcctgggtg acgacgtctt caccgtctgg tcttcggtag
     4321 ggaggttcct ctctctcctt gatgccgttg ccgcctcccc cggccgtggg gatctcctca
     4381 gcatatcgga tatcggttat caggacgggg ttgagcagcg gctggccagg gcgcgttcaa
     4441 acggctggcg gatcgagtgg cgcgatcatc accgctggac gaaggacgag atccgcacag
     4501 tcgataagag gacggacatc ctccatatcg acgttgccac ctgtgcaacc gggatcgtcg
     4561 cccgcgacct tgctccaggc gatcccgtgg cagaagagat tgcgcgtgtc gtctgcgact
     4621 acgacctctg gaagcacctg gacccaaggt cgaagatgct cggccaggtg gtgatgcgga
     4681 agggtctcag ggattacgtc cgcgacaact ttgtccgggg catcatcctc gatgagcgta
     4741 tcgagaacga atatgctgag atcctccggg agatggagca tgacatcaga aggagcatcc
     4801 ggcatgccac cgtcatccag ggcggccgct acaggattgt tttcgctcct ctctatggct
     4861 acccaagtga gactgcccat gccatccgcc aggagctggg gagcgatatc gaggtgatcg
     4921 tctcttcaaa cggccgcatc tccattcgtt ccgtcccgcc gataagccat cttgtcgccc
     4981 ggcagttctc cggtgggggt catcctcacg cggcaggggg gacgttcacc ttcagcctgc
     5041 ttgaccgctt tctattctgg ctgatcaagc ggaaccgcca ttaccgcgac cttgcggcgg
     5101 ttgcggagtc tatcgaggaa tgaacgctct gtccagaagt ctcttccagt agtcagggca
     5161 ggcggtatct gcacagtagc cacccacctc gaggctatca gcgtcgacta tcccggccgc
     5221 cgccacaact tcccctccct ccgggttgag caggattaac ttccggagac ggaaccgttc
     5281 cataaggtcg ggaaggcggt cgagatcagt cgccgggagg atgagagagt cctggtactc
     5341 gagcaggagt tcaaggtcga acctcgccgg gttgcgaggg aagaagagga cttttctgcc
     5401 agccagccgc tcgagttcga tcagatcagg tgtatccgct atcagaacgt gccccgcgag
     5461 accctggtcg cgcatatccc gcatgagtcc ggcgtagagg tctgagatag tctcggagaa
     5521 ctcttcccgg tccccgatgt agtcgtcccg gaaccccatg atgtgcggtg caggaagagc
     5581 aatcctggcg tctttacatc tctcccgcac aaacagggca tctgcggtca cggccgccgg
     5641 ttcgacaccg ggttcgtcga tcagcactcc gtccttcatg ctggtaaaga cctcccgtaa
     5701 ccgttcggca tagaagaggc cgccagcagc cggaattgag acaccgtcct gggcgtcaag
     5761 cgagcgttcg agccggtagg tggtaagatc cacctctgtc ccggttttcc ctttcagcca
     5821 ttccgcaagt tccgggacgc caggcctccc gacctctgag ccaaacgatc tcaccggtat
     5881 tctgatttgt cgtgccatgc gctggtatct ttattagcat cgcaacgaga gtagataagg
     5941 atgagtggta agccgttgct ggtaacgtgc gggctcccgt acacgaatgg tccctgccac
     6001 attgggcacc tgcggaccta cgtgccggcg gacttttacg tccgctatat gcgccggtcg
     6061 ggagaggagg tcgcttttat atgtggttct gataaccatg gaacgccgat cgtgatcagc
     6121 gccgagcagg agggctcgac gcccagggca ctctcggagc gctatcacga gcatttctat
     6181 gatacgttcc gacggatgga cgtcgtcttc gaccactttg gcatgaccga cgactccatg
     6241 aaccatgaga ccacccgctc gatcgtgcag cggctgatag ataacggcta tgtctacgaa
     6301 aagaccgtca gccagagtta ctgcccgcag tgcgagcggt ttctcccgga ccgctatgtg
     6361 gagggtgtct gccctcactg cggggcgatt gcgaggggcg atgagtgtga ccagggctgc
     6421 ggacagcacc tggagcctgg tgaactcagg gatgcggtct gcaagatctg tggcagcaag
     6481 gctgaatatc gcgaacagga gcactatttc ttcaggcttt cggcattccg ggacttcctc
     6541 ctggactacc tgcccaccct tcggggcacg gccaccgcca ggaactacgc tctcggctgg
     6601 gtgaaggaag ggctgcgcga ctggtgcatt acccggacgc ttgactgggg cgttaaattt
     6661 ccggggcgcg acgaccttgt cgtctacgtc tgggtggacg cgccaatcgg ttacatctcg
     6721 ttcacgaagg agtgggcaaa cgccgccggg gtcgactgga aagactactg gtgcggcgat
     6781 actacaaggg ttactcactt catcggcggg gatatcatct accaccactg catcttctgg
     6841 ccggcgctcc tcaaaggggc aggatacggt ctcccgcacg cggtggttgc aagcggcatg
     6901 gtcacaatcg atggcaggaa gttctcgaag tctcgcgggt atgtcgtctg gacaaacgat
     6961 gactacctgg accaggggct ccctgcggac tacctccgct actacctcct ctcctacacc
     7021 agccacacga aggagctgga cttctcgtgg aaggtctacg ccgagcgggt gaataatgag
     7081 atcgtgggga cgctcgggaa cttcatatac cggacgatgt acttcgccga gaaggaattc
     7141 tctggtgtgc cggacgttgc tccacaaccg ggtatcatcg aggagatcga gcgctcgctt
     7201 gccgcgatag atgcccggat gcgcgattac gacttcaagg gtgccgtcga tggtatgatg
     7261 agcctggcct cgttcgggaa cgcttacatc cagaacaacg ccccctggaa actgatcaag
     7321 gaggatcagg ccgctgcaaa gcaggttatc gctgactgtc tccagatggt caaggccctt
     7381 acagtggtct tccagcccct gatgccaggc tccatgcagc gggcgtggac gatgctcggc
     7441 tacggcgacg atctcgcggc acatccgatc gctgaggcta cagcgccgat tggggcgaga
     7501 gcacttggaa aacccgttcc actgtttaaa aagatcgaga aagaacaggt cgaggcgctt
     7561 gagaaggtcc tggaggcgcg ggttgcgaag gccagtgagg ctgcaggggc aaaagcaccg
     7621 acagtctcca tcgatgagtt ttcaaaactc gacatccgca tagcaagggt tgttgcggct
     7681 gaaccgatcg atggatcaaa gaaactctac aaactcatcc tcgacgtcgg tggcgagaaa
     7741 cgccaggtgg tcagcgggat cgcgcagttc tacaccccaa aagaccttgt gggcagagag
     7801 gttgctgtgg tcgtaaacct cgccccggca aagatctttg gggtcgagag cagggggatg
     7861 atacttgccg ccggtgacga ggcctcgctt cttgtccccc tccgtccagt cagcccgggg
     7921 acgaaggtgc ggtgagaggg tcaggactga tacaatcatt tttccgaggc cgtcaaatca
     7981 acggttctgt gatataaacc ccccatcttt tcccgttat
//
LOCUS       DAOSZX010000068         8007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7682_length_8007_cov_21.868209, whole genome shotgun sequence.
ACCESSION   DAOSZX010000068 DAOSZX010000000
VERSION     DAOSZX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7682_length_8007_cov_21.868209"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1091)
                     /locus_tag="PK196_06215"
     CDS             complement(3..1091)
                     /locus_tag="PK196_06215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HON41047.1"
                     /translation="MSREEMDARAKQRLVGIAREWYEQMRDGVVPYIRLPTRTKRNLE
                     YDDDSEVWKYGDRESTRSAATEKSAIHLLKMAYVIGFLKQQLVENRSSTLRELYYISE
                     GWKKAKFAAQDESNLLIEDLEIITEMQREAFHLRPEEDGASIFGPLRLRETTRRGVKE
                     IHCQEDVGEAGYPIPNNVDNLEFIDHDAKFVIAIETGGMYARLMENGFDQDYGAILVH
                     LKGQPARSTRRVLRRLNESLGLPVVVFTDGDPWSYRIYASVAYGSIKSAHISELLATP
                     QAQFVGVQPSDISDYNLPADRLTEQDINALKAELTDPRFATDYWHNQINLQLDMRLKS
                     EQQAFASRGLDFVTKEYLPTRLSEMGVI"
     gene            complement(1084..2886)
                     /locus_tag="PK196_06220"
     CDS             complement(1084..2886)
                     /locus_tag="PK196_06220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HON41048.1"
                     /translation="MVIAEDLAREQRSISVAEFFEKNKHLLGFDSPTRGIITTVKEAV
                     DNALDACEEAEVLPDIFVGVKRVEDEVYRITVEDNGPGIVPENVPPVFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTTGTPARITSRTGAKTPAHRFEVMIRTETNEPEVIS
                     HEKIEWDRTHGTRIEIQFRSTLAAKKRLLDYLRLTAIVNPHARITADIDGEITVFERA
                     SDEVPPCPKPVLPHPHGIEFGALKRLAAASTGTVEEFLVGSFSRVGKKTAEEMIGLAG
                     LKPSRKANKLDSNELKRLLEAMQAVRVPAPPAQQCLSPIGEDLICRGLEKEIHLDFIK
                     ARTRPSAVYGGHSFIVEAAIGYGGRIPPEGNARILRFANRVPLLYQQGACAITDCVSQ
                     VNWKNYGLAQQGLPVGPVLIMVHVASTNVPFTSESKDAIASIPEIEREIVLVLQELGR
                     ELKRYLSRREKKKLQDDRARAVCSIIPEIAAKVSEIVELPPVDTTPIEGKIMRKVVAK
                     KRTVDGKVSIEVNNYTGKEVEIVIYSLSRDAAEDAVPRPDFVDSIDGEYNKVWRCTLG
                     AGEAWRALYTGTGEGTLDLRGVDDGMKVVVDLDV"
     gene            complement(2873..4105)
                     /gene="hisS"
                     /locus_tag="PK196_06225"
     CDS             complement(2873..4105)
                     /gene="hisS"
                     /locus_tag="PK196_06225"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HON41049.1"
                     /translation="MLQKPRGTRDFLPEEMEQRRSIEQRMREVARRWGYREVCTPDFE
                     HLELFTMKSGEGIIQEMYVFEDKSGRKMTLRPEVTAAVLRMYVNEGRVLPKPIRWCYF
                     ADCFRYERPQKGRYRQFWQFGAELIGADTALAEAEVIMLADDILRSTGLTFDLHVGHL
                     APMKHILSGLDPADQGVIMACLDKHDRVGLEAVLAARRLTHLAEPLAALSDCSSVEEV
                     FEVAGNVPERARIEEMFAILDSQEIRYRPDFGIARGLDYYTGTVFEGFARNLGAENQI
                     LGGGAYRLAHLFGGDDVASCGFAIGFDRVMVSLGGVELPHESVVGVVCTPGARGRALQ
                     VARAFREAGIRAEADLMERGLAAQVAHAAKTAEFVVIIGEREVEAGTVTLKDLRTGEQ
                     QEMELEDAIAGVAGHGHR"
     gene            complement(4122..4454)
                     /locus_tag="PK196_06230"
     CDS             complement(4122..4454)
                     /locus_tag="PK196_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41050.1"
                     /translation="MSYLVYIAWFGVAVTVFLWLRDLRIFYRTGLPSYRKAAYHGVPY
                     TALALLGAFITMYAEGWDLLGLALILLALYLQDRVERENIWHGKSAVERFFGRAERRN
                     VKSSEKRP"
     gene            4503..5276
                     /gene="larB"
                     /locus_tag="PK196_06235"
     CDS             4503..5276
                     /gene="larB"
                     /locus_tag="PK196_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HON41051.1"
                     /translation="MLPNATIREILRMYRDGEVSEDEAADAIEGLRIDMIDGIARFDT
                     GRGVRCGVPEVVLAEGKDPEAFAGIMIAQVRAAGRCIATRVSPAHLEALRARLPPGVR
                     LEHREAARGVILSTGEDPAPRGGTVAILTAGTSDIPAAEEARLMAEEMGCSVKTAYDV
                     GVAGIHRLFSALRDLIPADVFIVAAGREGTLPAIVAGLVDSPVIGLPVSTGYGYMGGG
                     EAALASMLQACSVLAVVNIDAGFTAGAFAAQVARGRARE"
     gene            5273..5779
                     /locus_tag="PK196_06240"
     CDS             5273..5779
                     /locus_tag="PK196_06240"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HON41052.1"
                     /translation="MSRGFYIGRFQPYHNGHHSVLERIARSVDEIVIGVGSAQLSHTL
                     DNPFTAGERVLMLTRSLTDLDCPFYVIPIEDIQRNALWVAHVRSMTPPFETVYSSNPL
                     VMQLFAEAGMAVQSPDMYERLTHSGTAIRRRMLDGEPWEHLVPPAVVEVIREIRGVER
                     LQRIARSD"
     gene            5823..7076
                     /locus_tag="PK196_06245"
     CDS             5823..7076
                     /locus_tag="PK196_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41053.1"
                     /translation="MFKQLRDLFHRAEPEEEHPELSFDGIPSWLDSREEEIARGLEAA
                     TASSRDAISATLVSLREAVARMETAETDDDTHPRLRDISRKTLPGFTRSMSQILSREP
                     SGDPETFYAAAAEILKGVLKTMKGQGKYLSSVYPGEVRELRDAVRDLGREVNAMTEAI
                     GRARERREQVEGVREAYEAIVRIWDDYAAVSANLRDHAAAIGELEDAIRKAGEGLAAL
                     RDHPDYARKIELEERIREIRAEEEMVGREIGSLRSTAAHLLRKAGKVAERSGDRAAAA
                     AISLAVDAEVGIEAAMPVVLDMVRRGDLALKNQEEIRLFSDPETLPAALRDAIERGRG
                     LEMERKKEEAALAALQVVVEEERLRAALPDLQKRHEAAVAAKARAENRLESLRAEYAR
                     ECENLRSRMTGFEARVTIADLPPQP"
     gene            7141..7989
                     /locus_tag="PK196_06250"
     CDS             7141..7989
                     /locus_tag="PK196_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41054.1"
                     /translation="MTTNTITPGPASRLWMAVPAVSFGGIGVGLLLMEAVGFSYGFWA
                     GIAGCVIASCLLFYQAYSKPRRDLVSLFTPLYAVLIFLIPNEVGSMVIVQVVFAATIS
                     LLSVRVEKLFNVKKTEKKTMKQMLNEYIMRIEPLLSRVDEETGHLVAQALLRFKFGLY
                     ESVIDNCNKALDRLKAIEPYPGVLERALLILRERASGLAVSRVVTYPEHVFTEEDSEY
                     LAIHLPESLVDDPAALDLDNALILLYAVGIETSPLDEQALEEHQRFIIQILESYKEKL
                     AEAAAT"
BASE COUNT         1445 a         2451 c         2511 g         1600 t
ORIGIN      
        1 ctctagataa cccccatctc cgacagcctt gtcggcaggt attccttcgt cacaaagtca
       61 agccccctgc ttgcaaacgc ctgctgttcc gacttcagtc tcatatcgag ctgcaggttg
      121 atctggttat gccagtaatc ggtcgcaaac ctcggatcgg tgagttccgc tttaagggca
      181 ttgatatcct gttctgtgag cctgtcggcg gggaggttgt agtctgagat gtctgagggc
      241 tgaaccccga caaactgcgc ctgaggcgtc gccaggagtt ccgagatgtg ggcgctcttg
      301 atcgaaccgt aggcaacgct tgcatatatc ctgtatgacc acggatcgcc gtcggtgaag
      361 accaccaccg gcagaccaag cgactcgttg agacgcctta gcaccctgcg cgtcgaacgc
      421 gccggctgac ccttgaggtg gacaaggatc gccccgtagt cctggtcaaa cccgttctcc
      481 atcaaccggg catacatacc gccggtctct atggcgatga caaacttcgc gtcgtgatcg
      541 atgaactcga ggttgtcgac gttgttcggg atggggtatc ccgcctcccc gacatcctcc
      601 tggcagtgga tctccttcac cccccggcgg gtggtctccc gaagcctgag cggcccgaat
      661 atcgaggccc cgtcctcctc cggacggagg tggaacgcct cccgctgcat ctcggtgatg
      721 atctcaagat cctctatcag gaggttgctc tcatcctggg ccgcaaactt cgccttcttc
      781 cagccttcgg agatgtaata gagctctctc agcgtcgacg accggttctc cacgagctgc
      841 tgcttcagga acccgatgac gtaggccatc ttcaggagat ggatggcgct cttctccgtc
      901 gccgccgacc gcgtgctctc ccggtcgccg tacttccaga cctcgctatc gtcatcatac
      961 tcaaggttcc gcttcgtccg cgtcggcagc cggatatacg gcacaacgcc gtcccgcatc
     1021 tgttcatacc actcgcgtgc gataccgaca agccgctgct ttgcacgcgc atccatctct
     1081 tccctagaca tcgagatcca ccaccacctt catcccgtca tcaacccccc gcaggtcaag
     1141 cgtcccctca cccgtcccgg tgtagagggc gcgccaggcc tcgccggcac cgagggtgca
     1201 ccgccatacc ttgttgtatt cgccgtcgat gctgtccaca aaatccggcc ggggcaccgc
     1261 gtcctccgcc gcatcgcggg agaggctgta gataacgatc tcaacctcct ttccggtgta
     1321 gttgttcacc tcgatagaga ccttcccatc gaccgtcctt ttctttgcaa cgacttttcg
     1381 cataattttg ccctcaatcg gcgttgtatc gaccggcggg agttcaacga tctcgctaac
     1441 ctttgctgct atctccggga tgatcgagca taccgcacgg gcacggtcgt cctgcagttt
     1501 cttcttctcc cgccgggaaa gataccgctt cagttcccgc cccagttcct ggaggacaag
     1561 gactatctcg cgctcgatct ccgggattga cgcgatcgcg tccttgctct cgctcgtgaa
     1621 gggaacgttt gtggaggcca catggaccat gatcagaacc gggcccacgg gaagaccctg
     1681 ctgtgcgagc ccgtagttct tccagttcac ctgagagacg cagtctgtga tggcgcaggc
     1741 gccctgctgg tagaggaggg ggacgcggtt tgcaaaccgg aggatcctgg cgttcccttc
     1801 tggcgggatc ctgcctccgt agcctatcgc cgcctcgact atgaacgagt gcccgccgta
     1861 gaccgcgctc ggccgcgtcc gcgccttgat gaagtcgagg tggatctcct tctcaagccc
     1921 cctgcagatc aggtcctccc ctatcggtga gaggcactgc tgcgccggcg gggccggaac
     1981 cctcacggcc tgcatcgcct ccaggaggcg cttgagttcg tttgagtcga gtttgtttgc
     2041 cttccgggag ggtttgagcc cggcaaggcc gatcatctcc tccgcggtct tctttcctac
     2101 acgtgagaaa ctgccgacca ggaactcctc caccgtcccc gtgctcgcgg ccgccagcct
     2161 tttcagtgca ccgaactcga tcccgtgcgg gtgggggagg acgggtttcg ggcacggcgg
     2221 gacctcgtcg cttgcccgct caaagaccgt gatctcaccg tcgatatccg cggtgatcct
     2281 tgcatgcggg tttacgatcg ccgtcagcct caggtagtcg agcagccgtt tcttcgcggc
     2341 aagggtgctc ctgaactgga tctcgatcct tgtcccgtgc gtccggtccc actcgatctt
     2401 ctcgtggctg atgacctcgg gctcgttcgt ctccgtcctg atcatcacct cgaaccggtg
     2461 ggcaggcgtc tttgcccccg tccgggaggt gatccgggcc ggggtgccgg tggtgagctg
     2521 cgcatacagc acagcagctg atatcccgat accctgctgc ccgcggctct ggcggatctg
     2581 gtggaacctg gaaccgtaga ggagtttccc aaagaccggg gggacgttct ccggcacgat
     2641 cccgggaccg ttgtcctcga cggttatacg gtagacctcg tcctcgaccc tctttacgcc
     2701 gacaaatatg tccggcagga cttcagcctc ctcgcaggcg tcgagggcgt tgtccaccgc
     2761 ctctttgacg gtggttataa tcccgcgtgt cggggagtca aaacccagga ggtgtttgtt
     2821 cttctcgaag aactccgcaa cgctgatgct tcgctgctct ctggccaggt cctcagcgat
     2881 gaccatgccc tgccaccccg gcgatggcgt cctcgagttc catctcctgc tgctccccgg
     2941 tgcggaggtc cttcagggtc accgtcccgg cctcaacctc gcgttcgccg atgatcacga
     3001 caaactccgc ggtctttgcg gcgtgagcaa cctgggcggc gagcccccgc tccataaggt
     3061 cggcctcagc ccggatcccg gcctcccgga aggcgcgggc aacctggagc gcccggccgc
     3121 gggccccggg cgtgcagaca accccgacaa cggactcatg ggggagttca accccgccaa
     3181 gcgagaccat gacacggtca aaccctatcg caaacccgca agaggcgaca tcgtcgccgc
     3241 caaaaaggtg ggcgagcctg taggcgccgc cgccaagtat ctggttctcc gccccaaggt
     3301 tcctggcaaa cccctcaaag accgtcccgg tgtagtagtc aagcccgcgg gcgatcccga
     3361 agtccggccg gtagcggatc tcctgggagt caaggatcgc aaacatctcc tcgatccggg
     3421 cgcgttcggg aacgttcccc gcgacctcaa acacctcctc cacgctgctg caatcggaga
     3481 gagcggcaag cggttcggca aggtgggtga gacgccgggc ggcgagcacc gcctcaagcc
     3541 ccacccggtc atgcttatcg agacaggcca tgatcacccc ctggtcagcg gggtcaagcc
     3601 cggagaggat gtgcttcatc ggggcaagat ggccgacgtg gaggtcaaat gtgagaccgg
     3661 ttgacctgag tatatcgtcg gcgagcatga tcacctcggc ttcggcaagc gcggtgtccg
     3721 cacctatgag ttcggcgccg aactgccaga actgcctgta gcggcctttc tgggggcgct
     3781 cgtagcggaa acagtcggca aagtagcacc agcggatggg ttttgggaga accctgccct
     3841 cgttgacgta catccggagc accgctgccg tcacctcagg ccggagagtc atcttcctgc
     3901 cgctcttgtc ctcaaagaca tacatctcct ggattatccc ctcgccggac ttcatcgtga
     3961 agagttcgag gtgctcgaaa tcgggtgtgc agacctcccg gtacccccac cgccgggcca
     4021 cctcccgcat ccgctgctcg atcgaccgcc gctgttccat ctcttcgggc aaaaagtccc
     4081 gtgtgccccg tggtttctga agcattcagt attctccctt gttatggtct cttctccgaa
     4141 ctcttaacat ttctgcgctc cgcccggccg aagaaccgct cgacggcact tttgccatgc
     4201 catatgttct cccgttcaac cctgtcctgg aggtagagtg cgagcaggat cagcgcaaga
     4261 ccgagaaggt cccagccttc tgcatacatg gtgatgaacg ccccaaggag tgcaagcgcc
     4321 gtatatggaa ccccgtgata cgccgctttc cggtaactgg gaagacccgt acggtagaat
     4381 atccgcaggt cgcgcagcca tagaaaaacg gtgacggcaa cgccgaacca tgcaatatag
     4441 acaaggtagg acatgatgtg atgtaatatt atacctccac cccatatttt tgttatcaaa
     4501 gtatgctgcc gaatgccaca atcagggaga tcctccggat gtatcgtgat ggcgaggttt
     4561 ccgaggacga agcggctgac gctatcgagg ggctcaggat cgatatgatc gacggcattg
     4621 cccggttcga tacggggcgg ggtgtgcgct gcggggtgcc agaggttgtg cttgccgagg
     4681 gcaaggatcc ggaagcgttt gcggggatca tgattgcgca ggtgagggcg gcgggccggt
     4741 gcatcgcgac aagggtctcg ccggcgcacc ttgaggcgct ccgggcccgg ctgccgccgg
     4801 gtgtccggct ggagcaccgg gaggcggcac gcggcgtcat cctctcgacg ggtgaggacc
     4861 ccgccccccg cggggggacg gtcgccatcc tcacggcagg gacgtccgat atccctgccg
     4921 ccgaggaggc gaggctgatg gccgaggaga tgggatgcag tgtcaagacc gcctacgatg
     4981 tcggggtggc ggggatccac cgcctcttct cggctctccg cgaccttata cctgcggacg
     5041 tcttcatagt ggccgccggc cgggagggga cgctcccggc gatcgtggcg gggctggtcg
     5101 acagtccggt gatcggtctt cctgtgagca ccggttacgg ctacatgggc ggcggcgagg
     5161 cggctcttgc aagcatgctc caggcgtgct cggtcctggc ggtcgtgaac atagatgccg
     5221 ggtttacggc gggagcgttt gccgctcagg tcgcacgcgg gagggcgcgg gaatgagccg
     5281 cgggttctac atcggccggt tccagcccta ccacaacggt catcactcgg tgctggagag
     5341 gattgcccgc tccgtggacg agattgtcat aggggtgggg agcgcccagc tctcccatac
     5401 gctggacaac ccctttacgg ccggtgaacg ggtgctgatg ctgacgcggt cgctcacgga
     5461 tctcgattgc ccattctacg taataccgat cgaagatatc cagcgcaacg ccctctgggt
     5521 ggctcatgtc cggtcgatga caccgccgtt tgagacggtc tactcgagca acccgctggt
     5581 catgcagctc tttgccgagg ccgggatggc ggttcagtct ccggatatgt atgaacggct
     5641 gacgcactcg ggcaccgcca tccgccggag gatgcttgac ggcgagccct gggagcacct
     5701 tgtccctcct gcggtcgtgg aggtcatccg ggagatccgg ggtgttgagc gcctccagcg
     5761 gatcgcaagg agcgattgaa gaaaagactg atggtgaccc ggatcatagt ctgagaagag
     5821 aaatgttcaa acaactgcgc gacctctttc accgggctga accggaggaa gagcacccgg
     5881 aactctcgtt tgacggcatc ccgtcctggc tggactcccg cgaggaggag atcgcccgcg
     5941 gccttgaggc tgcgacggca tcgtcacggg acgccatctc cgccacgctg gtctcgctcc
     6001 gggaggcggt cgcccggatg gagacggcag agaccgatga tgatactcac ccccggctca
     6061 gggatatatc gcgaaagacg ctccccgggt tcaccaggtc gatgtcacag atcctttcac
     6121 gcgagccgtc cggcgacccg gagaccttct acgcggcggc cgcggagatc ctgaaggggg
     6181 tgctcaagac gatgaagggg caggggaagt atctctcgtc cgtttacccc ggggaggtca
     6241 gggagttgag ggatgctgtc cgcgaccttg ggcgcgaggt caacgcgatg acggaggcga
     6301 tcgggcgggc gcgtgagcgc cgggagcagg tggagggggt gcgggaggcg tatgaggcca
     6361 ttgtccggat ctgggatgat tatgctgccg taagcgcaaa tctccgagac cacgccgctg
     6421 cgattggaga actggaagac gcgatccgga aggccgggga agggcttgcc gccctgagag
     6481 atcacccgga ttacgcccgg aagatagaac tggaggagag gatccgcgag atcagggcgg
     6541 aagaggagat ggtcggaaga gagatcgggt cgctccggtc cacggccgcc catcttctgc
     6601 gaaaggccgg gaaggtggca gagaggagcg gagaccgggc tgctgctgcg gcgatctccc
     6661 tggcggttga tgcggaggtg gggatcgagg cggcgatgcc ggtggtcctg gatatggtcc
     6721 ggcggggcga tctcgccctc aagaaccagg aggagatccg tctcttctcc gacccggaga
     6781 ctctgcccgc tgcgctgagg gacgctattg aacgcgggcg tggccttgag atggagcgaa
     6841 agaaggagga ggcggccctg gcggcgctgc aggttgtggt tgaggaggag cgtctcagag
     6901 cggcgctccc ggacctgcag aagaggcatg aagccgcggt cgcggcgaag gcgcgcgcgg
     6961 agaaccgcct tgagagcctg cgggccgagt atgcgcggga gtgcgagaac ctgcggtcac
     7021 gcatgacggg atttgaggca agggttacga tcgccgacct tccgcctcaa ccctgagcac
     7081 gcggtagaga atggaaacca tgtttatctc cggcggcgaa atgacttgtg agaggagtcg
     7141 atgaccacaa acaccataac acccgggccg gcgtcacgtc tctggatggc ggttcctgca
     7201 gtctcatttg ggggtatcgg ggtcggtctg ctcttgatgg aggctgtggg gttttcctac
     7261 ggtttctggg cggggattgc cggctgtgtg atcgcttcgt gccttctctt ttaccaggcc
     7321 tactccaagc cgcggcggga tcttgtctcg ctctttacgc cactttacgc tgtcctcatc
     7381 tttcttattc caaatgaggt cgggtcgatg gtgatcgtgc aggtggtctt tgcggcgacg
     7441 atcagccttc tttctgtccg ggtggagaag ttgtttaacg tcaaaaaaac ggagaagaag
     7501 acgatgaaac agatgttgaa tgagtatatc atgcggatcg agccgctcct ttcgcgggtc
     7561 gatgaggaga ccggtcacct tgttgcgcag gctctgttga ggtttaagtt tggactgtat
     7621 gagtccgtga ttgacaactg caacaaagcg cttgaccggc tcaaggcgat cgagccatac
     7681 cccggggtgc ttgagcgtgc gctcctgatc ctgcgtgagc gtgcgtcggg tcttgccgtc
     7741 tcccgggtgg tgacataccc cgagcacgtc ttcacggagg aggacagcga gtatcttgcc
     7801 atccacctcc cggagagcct ggtcgacgat cctgccgcgc ttgacctgga caacgctctc
     7861 atcctgctct acgcagtcgg gatcgagaca tcaccccttg atgagcaggc gctggaggag
     7921 caccagcggt ttatcatcca gatccttgag tcgtacaagg agaagctggc cgaagcggcc
     7981 gcgacgtgag cggggggctg gcggcgg
//
LOCUS       DAOSZX010000069         7900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7829_length_7900_cov_24.328362, whole genome shotgun sequence.
ACCESSION   DAOSZX010000069 DAOSZX010000000
VERSION     DAOSZX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_7900_cov_24.328362"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1085)
                     /locus_tag="PK196_06255"
     CDS             complement(42..1085)
                     /locus_tag="PK196_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HON41055.1"
                     /translation="MTDVFAFRSNDYYLSLIDWDDPADPIRRLVIPAIEELEPWGRLD
                     PSSEQSYTRVPGLQHKYRQTALLLVSDLCGGFCRYCFRKRLFIEDAREVNRDVSAGIA
                     YIREHPEITNVLLSGGDPLILETDKLREIVRQVREIDHVRIIRIGTKMPAYNPCRIIN
                     DPALLEMIRDYSLDEKRIYIMAQFSHPRELTDAACTAVSLLKEAGAVIMNQTPLIRGI
                     NDDPEVLADLFDKLSFVGIVPYYVFQCRPTVGNRPFAVPVEESYRIFERARSLCSGLA
                     KQARFVMSHATGKIEVLGMTDDYTYFKYNQAAKPEDIGRFMVYRSNPAAYWFDDYTEL
                     VDEGRVREPEDLM"
     gene            complement(1550..1924)
                     /locus_tag="PK196_06260"
     CDS             complement(1550..1924)
                     /locus_tag="PK196_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HON41056.1"
                     /translation="MPHKCTQCGREFEDGSTKILKGCPSCGGKKFLYIREAERHEDVM
                     KEKTPAEIAQEKGEEVLEVRDDRRKKEIEVYDRIESIRILGPGSYELNIEKLARSGEV
                     VLGLEKEGKYVVDILSMAKKKK"
     gene            complement(1945..2313)
                     /locus_tag="PK196_06265"
     CDS             complement(1945..2313)
                     /locus_tag="PK196_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HON41057.1"
                     /translation="MIQGVQIDLISAERLDKLTVMEKIHLILDEVMEGNIVVLEKGLA
                     PEEQSKLIEVTMREISPDGFAGIEMETYPVKETSGGFLGKLFGGRRPEARLTVIGPAN
                     QLKTLRKDKDLISAWVSSSR"
     gene            complement(2319..2960)
                     /locus_tag="PK196_06270"
     CDS             complement(2319..2960)
                     /locus_tag="PK196_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HON41058.1"
                     /translation="MAFFVNTRKKISRFLKAFFRKRTSRIGIYGPPNAGKTTLANRIV
                     RDWVGDAVGPVSEIPHETRRVRRKENITITGQNGSSITFDIVDTPGVTTKIDYNEFME
                     YGLEKEEAIKRAREATEGVAEAMHWLREDIDGVIYVLDATQDPFQQVNIMLVGIIESR
                     NLPVLIVANKTDLPDASPARIKSAFPQHPVVPISSLEGTNMEELYEKMTEYFG"
     gene            3063..3452
                     /locus_tag="PK196_06275"
     CDS             3063..3452
                     /locus_tag="PK196_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON41059.1"
                     /translation="MGRSGKSPVLPPGMKVEPPFLAVMYIIPGIFYYSYLSYKAESAG
                     TNFIQIDPRNTTQMCSNCGSIVKKDLSVRVHNCPDCRFAADRDYNAAVNIHRVGTEQP
                     FGPVEPGPLHHISVVQVPAMEQEAPHR"
     gene            3578..4141
                     /locus_tag="PK196_06280"
     CDS             3578..4141
                     /locus_tag="PK196_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41060.1"
                     /translation="MMNNSNAIRFETRIPLREVMQTHPATIDVGETVARAAQTMCRAG
                     VGSCIVLQNSLPTGIVTEEDINCKVVAKNLKPGEVRVSEVMSTPLITIGADKLVEDAA
                     AMMVKHRVRRLPVVEDQMVIGIVTVRDILTVATEVNELLADLIEINREEVYAMGVCDR
                     CGNMSDALSRVDNLMLCPACREEEQLL"
     gene            4138..4896
                     /locus_tag="PK196_06285"
     CDS             4138..4896
                     /locus_tag="PK196_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41061.1"
                     /translation="MIVAADLMVEIPALRYDDHVTKARSILRDDVFREVYIVDDRDKL
                     LGYIDISDVLRVVDTKSNITVEGFVREAAQVTPDTPLTEVSSAILNARTNSAAVVDKD
                     AAYLGGVLFSEIFPVLLSQRVNDGRVKDAMSHHPVTCSPGDPVHRIYTLIVASGFNSF
                     PVVHKDRVIGIISRRDLLRAGKVRTSVKNQADTTVERVMTTPVITVTPDDSLATAGRL
                     MVEHDVSMLPVVDEKKRLVGVIDRHDILGYTRAK"
     gene            4935..5882
                     /locus_tag="PK196_06290"
     CDS             4935..5882
                     /locus_tag="PK196_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41062.1"
                     /translation="MQPNRSNSEKRPADRLLKMPGKHDRGPIDFKTRIAEHEGEIMAI
                     ATRDVVVAHQTTTIIQAVEIMTREGFRRLPIVDAGTHRLRGIVTGSDIIDLMGGGDKY
                     NLVQVKHGGNFLAAVNEELREIMTEHPVTMPVTGSITDAVDIIVNRKIGGIPITDNDG
                     ELKGIVTERDVMMVLATEHSRCRVEDIMTSSVRVTTPDTPISKVCEKMVKCRFRRLPV
                     VTDDVLCGIVTATDIMRYLGEGKAFEHLTTGDAAEVMATPVRLLLSGELYTTTPYKNI
                     HEVALEMLRRQVGALPVIEDSHLVGLVTEYDLVKAFSRE"
     gene            5890..6729
                     /locus_tag="PK196_06295"
     CDS             5890..6729
                     /locus_tag="PK196_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41063.1"
                     /translation="MRAVDLMSSPVYVVTTTDNVAYARNVMLKHRISRLPVMEGEELR
                     GIITKKDIAYRLRQAEPIWRRRPIDRIPVEVLMVPDPITITPETSTRDIAAIMLDKNI
                     SGVPVVENGTVAGIVTKRDIMRSAYLRGLKATVGEVMGEAVTVNRYHSLDHIIETMKS
                     KNDKIIVVNDDGSLAGIITETNLAFYDYLNERMELPRKEVKLLRRSEPAGQKRYRYVV
                     EVSAVAEDVMSRPVITVAPDAPLQDAVGLMLEKEINSLVVVEDGEIRGLLKRDDIIKE
                     VAK"
     gene            6726..7508
                     /locus_tag="PK196_06300"
     CDS             6726..7508
                     /locus_tag="PK196_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41064.1"
                     /translation="MSEKFQLLVKDVMAKPVTIAKSAFVSEALNKMLDEGVDPLIVTN
                     NGMVIGTTSRAAIAESLGSRKTQSLKATSIHVANTVKEDFTSVYPDQSIDVLVPLLQH
                     YKVVVVFDSDHRLIGQVTAGDLLKVLRPAGGILDVMEPVYTIQGDERAVHLRRRMLDE
                     GIDRFIVEEGDTVIGIVTETDVARAFHALKDLVEGTHQDYRVRNLLVRDIMSTPLITV
                     DADADVSDVIDLMLKKNISSVPVKQNGRIVGIVTRNSLVHAL"
BASE COUNT         1838 a         2282 c         2131 g         1649 t
ORIGIN      
        1 cgtaaaaaga gagtccagac cgagcaatgc ggccatgggg cctacatcag gtcttccggc
       61 tctctcaccc tcccttcatc caccagttcg gtgtagtcat cgaaccagta ggccgccggg
      121 ttgctcctgt agaccatgaa ccgcccgata tcctccggtt ttgccgcctg gttgtacttg
      181 aagtaggtat agtcatcggt catccccaga acctcaatct ttccggtcgc gtgcgacatc
      241 acaaacctgg cctgcttcgc aagccccgaa cagagcgacc gcgcccgctc aaagatccgg
      301 taagactcct ccaccggcac cgcaaacggc cggttcccga ccgttggacg gcactggaag
      361 acgtaatagg ggacgatccc gacaaacgag agtttatcga agagatcggc aaggacctcg
      421 gggtcgtcat tgatcccgcg gatcagtggc gtctggttca tgatgaccgc cccggcctcc
      481 ttaaggagag agaccgccgt acacgccgcg tcggtcagtt cccgcggatg gctgaactgc
      541 gccatgatgt agatccgctt ctcatccaga ctgtaatcgc ggatcatctc cagcagcgcc
      601 gggtcgttta tgatcctgca aggattgtat gccggcatct tcgtccctat ccgtatgatc
      661 cgcacatgat cgatctcacg gacctgacgg acaatctcac ggagtttatc cgtctccagt
      721 atcagcggat cgccgccgct caagaggacg ttggtgatct cgggatgttc acggatgtag
      781 gcaatccccg ccgagacatc cctgttcacc tcacgcgcat cctcgatgaa gaggcgtttc
      841 ctgaaacagt agcggcagaa cccaccgcag agatcgctga caagcagcag agcggtctgc
      901 cggtacttgt gctgcagccc cggcacccgg gtgtaactct gttccgatga cgggtcaagc
      961 cgaccccagg gctcaagttc ttcgatcgcc ggtatgacca gcctgcggat gggatccgcc
     1021 gggtcatccc agtctatcag cgagaggtag tagtcgtttg aacggaacgc aaagacgtca
     1081 gtcacctccg caagacgggc acactcctcc gggctgatac caggaacctc atcaagcgat
     1141 gtgatgtaaa tcgggtttct ggtgttcgcg gttggatttt gaagatcaga tttcgaagtc
     1201 gtaaaacacc tccagagtga cactctccaa tgcaggatgt ggtctgaaat atcggggaca
     1261 ggatgcacga gagcagggat cgcaaggctg cgatctaccc aaaggctgct acaggcgtat
     1321 catcgaactc atgcaggaaa tcgtcgcacc ggatcagtca tcgagtgtcg gattatgttc
     1381 tatagattca ggggggctaa tggtatttat acgttgggct gaaaccgcaa cccctatgat
     1441 aaatacccac gtttcaccca ataaaaacga tacaacgacc aatacaagcc gctttccaga
     1501 gactcagcag cgagcaaata tgaaaaaatt ttgggcgaac actatcgcac tacttctttt
     1561 ttttggccat ggaaaggata tccacaacat atttcccttc cttttccagc ccgagaacca
     1621 cctcgcctga cctggccagt ttctcgatat tcaactcgta cgaccccggg ccaaggatac
     1681 ggatgctctc gatccgatca tagacctcga tctccttctt ccgccgatca tccctgactt
     1741 caagaacctc ctcccccttc tcctgggcaa tctccgccgg cgtcttctcc ttcatcacat
     1801 cctcgtgacg ttcagcctcg cggatgtaga ggaacttctt tccgccgcag cttgggcacc
     1861 ctttcagtat ctttgtcgaa ccgtcctcaa actcccgccc gcattgagta catttatgag
     1921 gcatatctca cctgcaattc tcattcacct cgacgacgaa acccaggcgc tgatgagatc
     1981 cttatccttc ctgagcgtct tgagctgatt ggcaggcccg atcaccgtca aacgggcctc
     2041 ggggcgtctc ccgccgaaaa gtttccccag aaacccacct gacgtctcct ttacaggata
     2101 ggtctccatc tcgatcccgg caaacccatc cggcgatatc tcacgcatcg tgacctctat
     2161 gagtttgctc tgctcctcag gagcaagacc cttctccagg acaacaatgt tcccctccat
     2221 cacctcatcc aggatcaggt gaatcttctc catcacggtg agtttatcaa ggcgttccgc
     2281 cgatatcagg tcaatctgta caccctgtat catctccatc acccgaagta ttccgtcatc
     2341 ttctcataga gttcttccat gttggtaccc tcaagactgg atatagggac cacagggtgc
     2401 tggggaaacg cgctcttgat ccgcgcgggg gaagcatccg gtagatctgt cttgttcgcg
     2461 actatcagga cggggaggtt cctgctctcg attatcccga caagcattat gttcacctgc
     2521 tggaatgggt cctgcgtcgc atcaagcaca tatataacgc cgtcaatatc ctcacggagc
     2581 cagtgcatcg cctcggccac gccctccgta gcctcccgcg cccgcttgat cgcttcctcc
     2641 ttctcaagac catactccat gaactcgttg taatcgatct tggtcgtcac accaggggta
     2701 tcgacgatat cgaaggtgat ggaactcccg ttctgaccgg tgatggttat attctcttta
     2761 cgccgcacac gcctggtttc atgagggatc tcactgaccg gcccgaccgc atcaccaacc
     2821 cagtcccgca caatccggtt tgcgagcgtc gttttaccgg cattgggagg accgtagatc
     2881 ccgatgcggc tggtccgctt cctgaagaac gccttcaaga accgtgaaat cttttttcgt
     2941 gtattaacaa agaacgccat caagctccct caggtagaca ggtttgcctg tacttacgtg
     3001 aggtgtggaa ctcacaaata aatatagtta tatgttcctt ccagcgcgtt tgcgggtgtg
     3061 aaatggggcg gagcgggaag tccccggtcc tgccgccggg gatgaaagtg gagccgccct
     3121 tccttgctgt gatgtatatc atcccgggta tattctatta ttcttacctc tcgtacaagg
     3181 ctgaaagtgc tggtacgaac ttcattcaaa tcgatccccg gaatacgacg cagatgtgct
     3241 caaactgtgg gagtatcgtg aagaaggatc tctcagtgag agttcacaat tgcccagact
     3301 gcaggtttgc tgccgataga gattacaatg ctgcggtgaa cattcaccgc gtgggtacgg
     3361 agcagccctt cgggcctgtg gagccaggac ctctgcatca catctctgtt gtgcaagtgc
     3421 cggccatgga gcaggaagcc ccgcaccgat gagcgcgggg tggttcactg tcaatcacct
     3481 cccgcgccgg ttaagagaat tcttttatac tgttgaatcc tagatagact tgcaccagga
     3541 gaattattta ttatagcggc cagatcagga gaatcccatg atgaacaata gcaatgccat
     3601 ccgcttcgag acacgcatac ctctaaggga ggttatgcag actcatccag cgaccattga
     3661 tgtgggagag actgtcgccc gggcggcaca gaccatgtgc cgcgccgggg tcgggagttg
     3721 catcgtactg caaaacagcc tcccgaccgg gatagttacg gaggaggaca tcaactgcaa
     3781 ggttgttgca aagaacctga aaccaggtga ggtccgcgta agcgaggtca tgagcacccc
     3841 gctgatcaca atcggcgccg ataaactggt cgaggatgcc gcggcaatga tggtaaagca
     3901 tcgcgtccgc aggctccctg tcgtcgaaga ccagatggtc atcgggattg ttaccgtgag
     3961 ggacatcctc accgtcgcga cagaagtaaa cgaactgctg gccgatctca ttgaaattaa
     4021 ccgtgaagag gtttatgcca tgggcgtctg cgaccgctgc ggcaacatgt cagacgcact
     4081 atcacgggtc gacaacctca tgctctgtcc tgcctgccgt gaggaggagc agttgctatg
     4141 atagtggccg cagacctgat ggtggagata cccgcgctgc gctacgatga ccatgtcacg
     4201 aaagcgcgca gcatactccg ggacgatgtc tttcgtgagg tctacatagt ggacgacaga
     4261 gacaaacttc tgggctacat cgacatctcc gacgtcctgc gggttgtcga caccaagtca
     4321 aacatcactg tggaggggtt tgtccgggag gccgcgcagg tcaccccgga cacgccgctt
     4381 acagaggtca gcagcgccat actgaacgcc cgcacaaaca gcgcagctgt cgtcgataaa
     4441 gacgctgcct acctgggggg ggtgctattc tcagagatct ttccagtcct cctctcccag
     4501 cgcgtcaacg acggcagggt aaaggatgcc atgtcgcatc accccgtcac ctgctcaccc
     4561 ggcgacccgg tgcaccgcat atacaccctc atcgtcgcga gcggctttaa ctcgtttccc
     4621 gtggtacata aagatagggt catcggcatc atatcccgcc gggatctcct gcgcgccggc
     4681 aaggtgcgca catccgtaaa gaaccaggca gacaccaccg tggagcgggt tatgacaaca
     4741 cccgttatca ccgtgacacc ggacgattca ctcgcaacgg cgggccggct gatggtcgag
     4801 cacgacgtca gcatgctgcc ggtcgttgac gagaagaaga ggcttgtcgg tgtcatcgac
     4861 cgtcacgata tcctgggtta cacccgggct aaataaccta tttcaggaaa taactccaga
     4921 aaggagggaa tatcatgcaa ccgaaccgca gtaattcaga gaagagacca gccgacagac
     4981 tcctcaagat gccgggcaag cacgaccgcg gcccgatcga cttcaagacc aggatcgccg
     5041 aacacgaagg cgagattatg gcaattgcca ccagggacgt tgtagttgcg caccagacca
     5101 caactatcat ccaggcggtt gagatcatga cccgggaagg gtttcggagg ctgccgatcg
     5161 tcgatgccgg gacgcaccgc cttcggggga tagtcaccgg cagcgatatc atagatctca
     5221 tgggcggcgg cgacaagtat aacctcgtcc aggttaaaca cggcggaaat ttccttgccg
     5281 ccgtcaacga ggagcttcgc gagatcatga cagagcaccc ggtcaccatg ccggttacag
     5341 ggagcatcac cgatgcggtc gatataattg tcaaccgcaa aataggcggg atacccatca
     5401 ccgacaatga cggagagttg aagggcatcg tgaccgagcg cgacgtgatg atggttctcg
     5461 caaccgagca ctcgcgctgc agggttgagg acatcatgac ctcctcagtc cgtgtaacca
     5521 cacccgatac ccctatcagc aaggtctgtg agaagatggt gaagtgccgg ttccggcgac
     5581 tcccggttgt cacagatgat gtcctctgcg ggatcgtcac cgccacagac atcatgcgct
     5641 acctggggga gggcaaagcc tttgagcacc tgacaacagg agacgctgcc gaggtgatgg
     5701 ccacaccggt gcgcctcctc ctctccggcg aactctacac aacaacacca tataaaaaca
     5761 tacatgaggt cgctctcgag atgctccgcc ggcaggtggg agcgcttcct gtcatcgagg
     5821 attcgcatct tgtgggactc gtgaccgaat atgatctggt aaaagcattt tccagggagt
     5881 gatgaaggca tgcgtgctgt tgatttgatg tcatccccgg tctacgtcgt tacaacgacc
     5941 gataacgttg cctacgcgcg aaacgttatg ctcaagcacc gcatatccag gctgccagtt
     6001 atggagggag aggaactcag gggtatcatc accaaaaagg atatcgccta caggctcagg
     6061 caggcagagc ccatctggcg gcggcggccg attgacagga tccctgttga agtcctgatg
     6121 gtgcccgacc cgatcaccat aacccctgaa accagcaccc gcgatattgc ggccatcatg
     6181 ctggataaga acatctccgg ggttcctgtc gttgaaaacg ggactgtggc aggcatcgtc
     6241 accaaacggg atatcatgcg atcggcgtat ctgcgggggc tcaaggccac ggtcggcgag
     6301 gtgatggggg aagcggtcac ggtcaaccgc taccactcgc ttgatcatat catcgagacg
     6361 atgaaatcaa aaaacgataa gattattgtt gtcaacgacg acgggagcct tgccggcata
     6421 attacagaga ccaatctcgc attttacgac tacctgaacg agcggatgga actacccaga
     6481 aaagaggtta agctcctgcg gagaagcgag ccggcagggc agaaacgtta caggtacgtc
     6541 gttgaggtat cggcggttgc agaagacgtc atgagcagac cggtcatcac cgtcgctccc
     6601 gatgcacctc tgcaggatgc agtcgggctg atgctggaga aagagatcaa cagtcttgtc
     6661 gtcgtggagg acggcgagat ccgggggctg ctcaagagag atgatatcat aaaggaagtg
     6721 gcaaaatgag cgagaaattt cagttactcg taaaagatgt gatggcaaaa ccagttacca
     6781 tcgcaaaatc tgcctttgtc agcgaagcgc tcaataagat gctcgatgag ggtgtcgacc
     6841 cgctcatcgt gaccaacaat ggcatggtca tcggcaccac atcccgtgcg gcaatcgccg
     6901 agtcgctcgg gagcaggaag actcagtcgc tgaaggccac atccatccat gttgcaaaca
     6961 ccgtcaagga ggacttcacc tccgtctatc ccgaccagag tatcgacgtt ctggtaccgc
     7021 tgctccagca ctacaaggtt gtcgtggtct ttgactccga ccaccgcctc atcggccagg
     7081 tgacggcggg cgacctcctg aaggtgctcc ggccggcggg aggcatcctt gacgtcatgg
     7141 aaccggtata caccatccag ggggacgaac gcgccgtcca cctccgccgc aggatgcttg
     7201 atgaggggat cgaccgtttc atcgtcgagg agggggatac cgtcataggc atagtcacag
     7261 agaccgatgt cgcaagagcg ttccatgcac tcaaggatct tgttgaagga acccaccagg
     7321 actacagggt caggaacctc ctggtgcggg acatcatgag cacgccgctg atcacggtgg
     7381 acgcggatgc agacgtctcc gatgtcatcg atctgatgct caagaagaac atcagctccg
     7441 tgcccgtcaa gcagaacggc aggatcgtgg gtatcgtcac ccggaactct ctcgtgcacg
     7501 ccctctgatc tggcaggcac cacccttttt tttgctgcct tctcccggcg aaacaacgtc
     7561 gccccgcgtg gagaaaaggc cggagtttca cgaagcggcg tgatcaacaa gcctcgtgaa
     7621 gaccctccgg ggatgggctg acggggccag gggggaggaa cttcccagac cccttgccca
     7681 tgcatgtcac caggaggcca tcaaatcaac ggttctgtga tataaactcc ccgtttcttc
     7741 ccggtattac gcgacgggga ctataaaacc agtttgctca tggtgtgatg ccccccataa
     7801 ccacggcggt gaagcgtctt ttgcggctga ccagccgccg cctggggggg ggaggggacg
     7861 ggacgtccct tccccctgtc tccgcgtgtc accgggaacg
//
LOCUS       DAOSZX010000070         7824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_7935_length_7824_cov_12.716695, whole genome shotgun sequence.
ACCESSION   DAOSZX010000070 DAOSZX010000000
VERSION     DAOSZX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7935_length_7824_cov_12.716695"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..3305
                     /locus_tag="PK196_06305"
     CDS             381..3305
                     /locus_tag="PK196_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON41065.1"
                     /translation="MTNGTPAAGVGGVRVELSVNGTDWSITPAEVVTGADGKATARFS
                     PGTKSGTARITATVLGLDEPLNDSVEQRIDHAAPYRLAGLWYDPEVNVSETTDIVLRM
                     EDRYGNPVDSRRVAENVTFMVGSPAGGAKFVGSGSDKATVGVDGTGNATARLRVDTVA
                     GENIVYIQPPASAGSGRYITITAAGGEPAGIYQTVSPDAEPLHYPELPLGGEFTITYA
                     LYDGYGNPAGDRKIKVDIRPISGSLLAGDLVVKTNSQGKAQIIYRSLDRAGVAEIVAT
                     AVDNNSVSCTKKVGFYSSTPVEMLITAVPGTIASRDVKEDIVSVIRAKVIDSKGNGVR
                     NETVSFKITDVNNTFPMSRVPEISNGTLNSTKDGTPIVATTDEEGIATINFHPGAFTK
                     DHDMATGNVTIQATWRGNNADTTVEFRNYPYLSINVTVDPEVVKVNDSVTVTIQLIGD
                     GYALQKRPIDVVLCTDRSGSMLQNTTTNPNYNRSNYEWVQQESVDDRMVHAMQAAKVF
                     VSQMESSKDRIGPVSFGQSGETDLDDYIYKFWAGNDYNWTSSEWVDDSSDDTDYITAH
                     YKNPQTYSASATRDLVLTYTYTNVNTTIEKWLPSGGTPMREGLYQAVQMIVENSRGKG
                     DPVKAIVLLTDGEWNTGGNPEGGWGAEWLSNVRTGSVIDYAKNKGIKIFTIALGNEPN
                     HDELSSYANKTGGKFYSATAGDNLTQVYEDIATKLQEAAGVNTTMNLVFGTVKVNSIP
                     TSDVFEYEYLNPVSTRMIKYWTDNKTTIWGPVCENQGTDWQGDGDKILSFDVGDIYLD
                     QTWETTFRLRVLKEGNIDIFGNGSVIRFNGTEGKSEVGLPHTFITARLNLTETDMSAA
                     QIWLEWKSVDIPEDKPGVLIPSWDLSYTGNRSVTQKILYQFSPDDIFWSGWHEADTKH
                     HPAGTNINGTYSSSIPLQENGGYYKVRVRAEENIPDGSSAQITSETLKVDKRDVAYIK
                     IE"
     gene            complement(4346..4417)
                     /locus_tag="PK196_06310"
     tRNA            complement(4346..4417)
                     /locus_tag="PK196_06310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4382..4384),aa:Arg,seq:tcg)
     gene            4473..4898
                     /locus_tag="PK196_06315"
     CDS             4473..4898
                     /locus_tag="PK196_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HON41066.1"
                     /translation="MILSSSEIVRRLHGGELVIDPYDAASQQPASYDLRACETTILPR
                     GECTLVASIERVELPEDLAGTLRCRSSFARRGVLLGGGFVDPGFRGHLTLCLTNAGRE
                     EIQVQEGDRVVQIVLHEVLNADRAYAGRYQDSVGVVHTR"
     gene            4895..5863
                     /locus_tag="PK196_06320"
     CDS             4895..5863
                     /locus_tag="PK196_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HON41067.1"
                     /translation="MTPSIRSERKYLEILRILAESHEPLGAKRLSEKMAERGFILSDR
                     AVQYYLQYLDETGLTAKVGNRGRLLTEAGIAESKRALVEEQIGFIISKLERLAFRSTF
                     DPVTGKGTVAYNLSLVREDDLPGVTAAFDEVASAGYGFLNTYRVVETDPRIPDGHIGI
                     MTACSITLDGVLQKMGIPTRLEYAGRIAVDENGSASFLDLIGYRGTSVDPLHLFVSAG
                     LTSINRLVTAGAGVALANVRAVPSAARSRVEEAVGLMEERGFNFPARRGIGEFNLPEH
                     PYRLFIVAFTGMNMVGNAVEKGYTVKTEIGAGTTPFEDLVGATGSR"
     gene            complement(5847..>6634)
                     /locus_tag="PK196_06325"
     CDS             complement(5847..>6634)
                     /locus_tag="PK196_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HON41068.1"
                     /translation="AGLEMRSGDYPVRNTAYVGIIVVLSGLSNIPRVGEIQDIRADYM
                     LECEEEASEDLEGDDHAPSFEVPSPDEAGYTGDETMMEKDEMIDMPAGAGTRQKDETI
                     DISPKPGKREEDGDLVLPPREGGREIDLAGSASVTSSVPAPKNSTFDLKGVRVEKTAP
                     RDDGMSGSASFKPVQRPKEGALSGEHVTFGQGQRPRDDLFDEAGVRMHGGVPAPKDRL
                     PDGAARRGPPRPKEVEPGRGKLRVLDAASREEEDPDDGITWIL"
     assembly_gap    6635..6734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6735..7774)
                     /locus_tag="PK196_06330"
     CDS             complement(<6735..7774)
                     /locus_tag="PK196_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HON41069.1"
                     /translation="MRVLTIGLGGAGSRVVDKLYDHDRRSGVHCMNAVVIDVDPNSLL
                     QLRHLPNDARIFFPSVNITDHAGAADVIDIDEVMTRLQRMDTIEIDSILICCGLGGSV
                     IDIAPFIIEKIRVSYVEPIFALAILPCLGEGKRVSAKAADDLEMLQELVDGVILFDNE
                     TWSRKILACERPESAGGSGRRRQPLTAPDPRTHYNMLNERIARQVGLLLRAGEFNEGG
                     VEVAEVVLDAGEVLNTLKEGGLVAVGYATERLPTGLTNLLRRRRSLKDLIQGSHERTA
                     RIISLAKRAVYEDISIPCDLTSADKALVLIAGPSAELSMKGFHAVRKWIDRSIAGLEM
                     RSGDYPVRNTAYV"
BASE COUNT         1716 a         2301 c         2212 g         1495 t
ORIGIN      
        1 tccagccagc gcaagggaag atcacgcggc ggccgcgaca gcctctagac cttccggtct
       61 tttttttacc ggccagggtt ttgcgggctc atctccgggg tttgagcacc gcaggactct
      121 gatgaccggt gtctgttttt caccccgccg ggggtgcgct gaaagaaact tcacccaatt
      181 ggcaaccttt atctctggat aatgaaacag gaaaagatgc agtgtgttgc atgaagaggt
      241 gggtgtatgc atgcctggtg atcgggctgg tcttgctggc ggcgccggtc gcggggcaga
      301 ttccggatgc agttgccgtc agcacggata ccgagtggct gacggcgggg agcggtgaga
      361 ccgctgccat cacggtgaac gtgacaaacg gcaccccggc ggccggggtg gggggcgtca
      421 gggttgagtt gtcggtgaac ggcactgatt ggagcatcac tcccgcagag gtggtgacag
      481 gcgcggatgg gaaggcaacg gccaggttca gccccggaac gaagagcggg acggcaagga
      541 tcacggcaac ggttttgggg ctggatgaac cgctgaatga cagcgtcgag cagcgtatcg
      601 accacgcggc gccctacagg cttgccggcc tctggtacga cccggaggtg aatgttagcg
      661 agacgacgga tatcgttctc cggatggagg accgctacgg gaaccctgtg gacagcaggc
      721 gggttgccga gaacgtgacc ttcatggtgg gctcgcctgc cgggggggcg aagtttgtag
      781 gaagcggcag cgataaggcc acggtggggg tggatgggac cgggaatgcc accgcgaggc
      841 ttcgtgtcga tacggtggcc ggggagaata tagtctacat ccagccgcct gcatcggctg
      901 gatcgggcag gtacatcacc atcaccgctg ccgggggtga gccggccggg atctatcaga
      961 cggtctctcc tgatgcagaa ccactacatt accccgaact gccgcttgga ggagagttca
     1021 ccatcaccta cgccctctat gacgggtatg gtaatcctgc cggtgatcgg aagataaagg
     1081 tggacataag gccgatctcc ggctccctgc tcgcggggga tctggtggtc aaaacaaaca
     1141 gccaggggaa ggcacagatc atctacagat cgcttgaccg ggccggggtc gccgagatag
     1201 tcgcaaccgc cgtcgataac aacagtgttt catgtacgaa gaaagtgggt ttttacagca
     1261 gcaccccggt tgaaatgctc atcaccgcag tccctggaac catcgcaagc agggatgtta
     1321 aagaagatat agtctctgtg atcagagcaa aagtaataga ctcaaagggc aatggcgttc
     1381 gaaatgaaac ggtttcgttt aagataactg atgtcaacaa tacattcccg atgagcaggg
     1441 tccctgagat atcaaacggg actcttaact ccactaaaga tggtacaccc attgttgcca
     1501 cgactgatga agaggggatt gccacgataa atttccaccc cggcgccttc acaaaagatc
     1561 atgatatggc cacaggaaat gtgacaatcc aggccacttg gcggggcaat aatgctgata
     1621 ccacggtaga gttcaggaac tacccctatc tcagcatcaa tgtcacggta gaccccgagg
     1681 ttgtgaaggt aaacgacagc gtgacagtaa cgatacagtt gatcggtgat gggtatgcgc
     1741 tgcagaaaag gccgatcgac gtggtgctct gcaccgaccg gtcggggagt atgctgcaga
     1801 ataccactac caatcctaac tataaccgta gtaattatga atgggttcaa caggaatctg
     1861 tcgatgaccg gatggtccat gcaatgcagg cggctaaggt ttttgtatcc cagatggaat
     1921 cttcaaagga tagaataggg ccggtatcgt ttggtcagtc gggggagaca gatcttgatg
     1981 attatattta taaattttgg gcggggaacg actataattg gacatctagt gagtgggttg
     2041 atgactcgag cgatgataca gactacatca ccgcacacta caaaaaccca caaacctact
     2101 ccgcttctgc cacccgtgac cttgtgttga catatacgta tacaaatgtc aacacaacga
     2161 ttgagaagtg gcttccaagt ggcgggacgc ctatgcggga agggctttat caggcagttc
     2221 agatgatcgt ggagaattcg agagggaaag gagacccggt aaaggcaatt gtcctcctca
     2281 ccgatggaga gtggaacaca ggtggaaacc ccgagggggg ctggggtgct gagtggttgt
     2341 ccaatgtgag aacgggaagt gttatcgatt acgcaaaaaa taaaggcatc aagatcttta
     2401 cgatcgcact cggcaacgag ccaaatcatg atgaactcag cagttatgcc aacaagaccg
     2461 gcggcaagtt ctacagtgcc accgcaggtg ataatctgac gcaggtctat gaagacatcg
     2521 ctacgaaact ccaggaagcg gccggcgtca acaccacgat gaacctggtg tttggtacgg
     2581 tcaaggtcaa ctccataccc accagcgatg tctttgagta tgaataccta aacccggtct
     2641 cgaccaggat gatcaagtac tggactgaca acaaaacgac catctgggga cctgtgtgcg
     2701 agaaccaggg cacagactgg cagggcgatg gcgataaaat actctcattt gacgtcgggg
     2761 acatatacct agaccagacc tgggagacca cgttccgcct gagggtctta aaagagggca
     2821 acatcgatat cttcgggaat ggatctgtca tccgattcaa tggcaccgag ggtaagagtg
     2881 aagttggtct acctcacacc ttcatcactg cccggctgaa tctcaccgag accgacatga
     2941 gtgcggcgca gatctggttg gagtggaaat cggtcgatat accggaggac aagcccggtg
     3001 ttcttatccc ttcctgggac cttagctaca ccggaaacag atcagtaaca cagaagatct
     3061 tgtaccagtt cagccccgat gacatctttt ggagcggctg gcatgaggcc gatacaaaac
     3121 accatcctgc aggcaccaat attaacggaa cctattcctc cagcatccct ctccaggaga
     3181 atggaggata ttataaagta cgggtccgcg cggaggaaaa tataccagat ggttcgagtg
     3241 cccagataac gtcagaaaca ctgaaggtag ataagagaga cgtggcgtat atcaagatag
     3301 aataaacttc tctcttcttt agagtttttt tagcgggaac gaacaacacg aacaaccagg
     3361 tcgtctgaga gtgcgttcat aaacgtttaa gcaagggctt ggctgctgat catcatgtac
     3421 ttccatgtgg aagagagagg tttaacaggt gttcctgaaa cagagcgagt aaacctggaa
     3481 cccatcagat caacaattcc gtgatataaa cctctccacc tcttcccggt agcatgtgcg
     3541 agttcaccga tgctctcgaa ccactcccgg tgagcgtctc ttccggtgac aacccccacc
     3601 gcccggccac taccgcggcc tcctcccgcc ccctcaagag ggggcgggca gtgagtggcg
     3661 attgacccgg atacagtgcc ccggcgtgga ggcaggatct tcggtgagtt tctcgcccga
     3721 aaccggctga agaaatctcc gccatggcag tgtatcgctg gagaatcacc cccgcggggg
     3781 tggggctgac ggggaggcgg agacggctcc cccctcccct gtctcctgca tctctggttg
     3841 aacggcattc atagaccccc accgcccggc cgcttgcgcg gcctcctccc gccccctcaa
     3901 gagggggcgg gcagtgcgtg gcgattgacc cggatacagt gccaggggcg gagacaggat
     3961 cttcggtgag tttctcgctt gaaaccggct gaagaaatct ccgccatggc agtgtatcga
     4021 tggagaatca cccccgcggg ggtggggctg acggggaggg gggaggcgga gacggctccc
     4081 ccctcacctg tctcggcgtg tcatccgaaa tcctctcttg cgtaaccccc atctctcgcc
     4141 gggataatga atccctcacg ccgccccccc cccgcatccc ttcatcaaat gccttcacca
     4201 gaccagcgca gccgtctcat aacctcgtgg aagaccggct ttgcaggccc ccccgcctga
     4261 acgatggcgt cacggcgtcc tgcagggagg gtgcgccttc cacgccggag tgaggatgaa
     4321 ccggcgcgaa aacggcaaaa aacagcggac ccagcgggag ttgaacccgc gtccttgggt
     4381 tcgaagccca aaaggatgtc ccctacccca tgggtcccct catcgatttc tatcataaga
     4441 tattaagcgt ttttatagca ctatcctata cgatgatcct ctcgtccagc gaaatcgttc
     4501 gacggcttca tggcggcgaa ctcgtcatcg atccctacga cgctgcgtcc cagcaaccgg
     4561 catcctacga cctgcgcgcc tgcgagacaa ccatcctccc gcgcggcgaa tgcaccctgg
     4621 tggcctcgat cgagcgcgtg gaactccccg aagacctggc ggggacactc cggtgccgct
     4681 cctcgttcgc ccgccgcggc gtcctcctcg gcggcgggtt tgtcgacccc gggttccgcg
     4741 gccacctgac gctctgcctt acaaacgccg gcagagaaga gatccaggtc caggagggtg
     4801 accgcgtcgt ccagatcgtc ctccatgagg tcctgaacgc cgaccgggcc tatgccggcc
     4861 gctaccagga cagcgttggc gtggtgcaca cccgatgacc ccctccatcc gctctgaacg
     4921 gaagtacctc gagatcctgc ggatcctggc cgagtcgcac gaacccctgg gcgcaaagag
     4981 gctgagcgag aagatggccg aacgcgggtt catcctgagc gaccgcgccg tccagtacta
     5041 cctccagtac ctcgacgaga cggggctcac cgcgaaggtc gggaaccgcg gccgcctcct
     5101 caccgaggcc gggatcgccg agagcaagcg ggcgcttgtc gaggagcaga tcgggttcat
     5161 catatcaaaa ctcgaacggc tcgccttcag gagcaccttt gaccctgtga ccggcaaagg
     5221 aaccgtcgcc tacaacctct ccctcgtccg tgaggatgac ctccccggcg tcacagcggc
     5281 tttcgacgag gtggcgtcag cgggttacgg gttcctgaac acctaccggg tcgtcgagac
     5341 cgaccccagg atacctgacg gccatatcgg gatcatgacg gcatgcagca tcaccctgga
     5401 cggcgtcctc cagaagatgg gcatccccac aaggctcgag tatgccggca ggatcgccgt
     5461 cgacgagaac ggttcggcaa gtttcctcga cctcatcggc taccgcggaa cgtcagtcga
     5521 ccccctccac ctcttcgtat ccgccgggct cacctccatc aaccggctcg tgacggccgg
     5581 agcgggtgtg gcgcttgcaa acgtccgtgc agtcccctcg gccgcacgct caagggttga
     5641 agaggctgtc ggcctgatgg aagagcgggg gttcaacttc ccggccagga gaggcatcgg
     5701 tgagttcaac ctccccgaac acccctaccg gctattcatc gtcgcgttca cagggatgaa
     5761 catggtggga aacgccgtcg agaagggcta caccgtcaaa acagagatcg gcgccggaac
     5821 gacgccattc gaagacctgg ttggcgctac aggatccagg tgatcccgtc gtctggatct
     5881 tcctcttcgc gggaggctgc atcaagcacc cggagtttcc cccgtcccgg ttcgacctct
     5941 tttggcctgg ggggcccgcg cctggcggcg ccatccggca gcctgtcctt cggtgcaggc
     6001 acccctccat gcatccgcac ccccgcctca tcaaagaggt cgtcccgcgg ccgctggccc
     6061 tggccgaacg tgacgtgctc accgcttaaa gccccctcct tcggccgctg caccggctta
     6121 aaggaggccg aaccgctcat accatcatcc cttggcgccg tcttctcaac acgaacaccc
     6181 ttcaggtcaa atgtgctgtt cttcggcgcc ggcaccgagg acgtcacaga ggccgacccc
     6241 gcaaggtcga tctcccgtcc accctccctg ggcgggagga cgaggtctcc atcctcctct
     6301 ctcttccccg gttttgggga gatatcgatc gtctcatctt tttgcctggt gccggcaccg
     6361 gccggcatat cgatcatctc atccttctcc atcatcgtct catcacccgt atatccagcc
     6421 tcatccggag agggaacctc aaaagagggg gcatgatcat ccccctcgag atcctcggat
     6481 gcctcctcct cgcactcaag catataatcc gcccggatgt cctggatctc tccaaccctc
     6541 ggtatgttag agagccccga gagcacgact atgatcccaa catacgccgt gttccgcacc
     6601 gggtagtcgc cggaacgcat ctcgagcccc gcgannnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnacatac gccgtgttcc gcaccgggta gtcgccggaa cgcatctcga
     6781 gccccgcgat gctcctgtca atccatttgc ggaccgcatg gaaccccttc atcgagagtt
     6841 cagccgaagg gccggcgatc agaaccagcg ccttatcagc gctcgtgagg tcgcagggga
     6901 tcgatatatc ctcatagacc gctcgctttg caagcgagat tatccgtgcg gttctctcgt
     6961 gcgagccctg gatcagatcc ttcaacgacc gcctccggcg gaggaggttc gtcaaccccg
     7021 tcggcagacg ctccgtggcg taaccgaccg cgacaagccc cccctccttc agggtgttga
     7081 ggacctcccc cgcgtccagg acgacctcgg cgacctcaac cccgccctcg ttgaactccc
     7141 cggcacgcag gagaagacct acctgacggg caatccgttc attgagcatg ttgtagtgcg
     7201 ttctcgggtc tggtgccgtg agaggctggc gccgccgccc ggaaccgcca gcgctctccg
     7261 gtctctcgca ggccagaatc ttcctcgacc aggtctcgtt atcaaagagg atgaccccgt
     7321 caaccagttc ctggagcatc tcaaggtcat cagcggcttt ggccgagacc cgtttaccct
     7381 cgccgaggca gggtaggatg gcaagggcaa agataggctc cacataggat acccggatct
     7441 tctcgattat gaacggcgcg atatcgatga cgctgccccc cagcccgcag cagatcagga
     7501 tcgagtcgat ctcgatcgta tccatgcgct gaaggcgggt catcacctca tcgatatcta
     7561 taacatctgc tgcacccgca tgatcggtta tgttgacgct cggaaagaag atccgggcat
     7621 cgttcgggag gtggcggagc tggagcaggg agttgggatc aacatcgata accaccgcgt
     7681 tcatgcaatg gaccccactc cgccggtcgt ggtcatagag tttgtccacc accctcgacc
     7741 cggcgccgcc aagcccgatt gtcagaacgc gcataccgat tatacccctt acctggcacc
     7801 ttccatgtac agccgaagag attc
//
LOCUS       DAOSZX010000071         7492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_8373_length_7492_cov_20.438080, whole genome shotgun sequence.
ACCESSION   DAOSZX010000071 DAOSZX010000000
VERSION     DAOSZX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_7492_cov_20.438080"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..751)
                     /locus_tag="PK196_06335"
     CDS             complement(119..751)
                     /locus_tag="PK196_06335"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HON41070.1"
                     /translation="MTVEKMTTRPFKKYELPPLPYAADALEPQISKEQLSIHHDRHHQ
                     GYVTGANANIERLEKARQEGAEVDMKALLKELSFNIGGHVLHTLFWPTMAPAGRGGGG
                     TPGGALADLIEKEWGSFDRFKAEFTKAAASVEGSGWAALACCPMTDRPIIMQIEKHNI
                     NVYPSFKILMVLDVWEHAYYVDYRNNRGQFIDAFWSVVNWDMVNKRLETL"
     gene            1036..1566
                     /locus_tag="PK196_06340"
     CDS             1036..1566
                     /locus_tag="PK196_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41071.1"
                     /translation="MSLERVNSPGRPAAGDAYITLHGRSTWALVNAYHAAVRERGLRP
                     ERVVIVTEEPYAEAASTAEAAIGMVSEGYGLSPEIQVEVLPEADFVGAGQMIRSLAGG
                     FIQQGYDVAIDITSGRKVTVAGALIALSVAGLDIRHIYYLAMKTADDIAKPYMMIPHQ
                     VQRIHDLIEDSAALSA"
     gene            1563..2570
                     /locus_tag="PK196_06345"
     CDS             1563..2570
                     /locus_tag="PK196_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41072.1"
                     /translation="MNDLIIREDELQVLINSLEEVHVSYPLYAGEIIVACPEGTGFSL
                     QMPAARETFRDWVAAYEPIAAELPSYADLQECMFAAGIARYTNQAAFDAMLVSYRNLK
                     KSVYFGLDTNLFYHGFASNNPGIDASTYLIVETVRDEISYAVNRKYSAAAIEEMTALA
                     PECRDYLADLENKRMKRSRKAAYLALKEYRSIRDRATEVPAPGPHTHLAEENDRNIVR
                     ALRKFEEERYALPVLLTADIYMVDLCMTEGLEYFYFDRPYRLEATTCTATAFRRLLFN
                     LAAVFGFVDCSGFTIFGEYGGKANDLDELKIRFRDEDIYRECRRELGICRRLAELGIT
                     R"
     gene            2534..3232
                     /locus_tag="PK196_06350"
     CDS             2534..3232
                     /locus_tag="PK196_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HON41073.1"
                     /translation="MQETCRTRNYAVEAVLCDMDNTLFDFVRAKREACRSVIDYLGRG
                     DPGTLFSQFLRGVHGFEDHENISDYLNDLGVYDGETFEVCCRIYEDVKLDLVEAYPGV
                     EETLRALQESGIGLAVVTDAESFQARRRLEKTGLIDYFETVVTPDLSGRCKPEPDSLL
                     YALRRLGAKPEEAMMVGDSAARDIAAGRRLGMVTAFAAYGDWRQSRVTDVGADIVLAE
                     FAQLRRYLSIPGKG"
     gene            complement(3239..4096)
                     /locus_tag="PK196_06355"
     CDS             complement(3239..4096)
                     /locus_tag="PK196_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HON41074.1"
                     /translation="MNEHDEHVIEAIGRCRVVVRNGQVVEVGRPVIRDCPLAKRFACP
                     VEDMTPEAIKRNIEARIRSFGMCTPAREVLAGPDFVIFGASELLSSAVRRGDLDAAVI
                     VSDGAGTVVATNPALIQGIGGRMSGLVKTSPILEVIARIQENGGAVLDTATAAIDQAA
                     GVALARGLGHRRVAVTTADAAEAAAIRERFPETVIVAVHTTGVSREDAALLAVTADLI
                     TACASRHIRDAAAKNALLQAGTSIPVFAMTPAGKGIILGKVAETDQQILVQGARLPVE
                     GPHLPSPLC"
     gene            4229..4387
                     /locus_tag="PK196_06360"
     CDS             4229..4387
                     /locus_tag="PK196_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41075.1"
                     /translation="MGRICSPFIVIECSRQCGFSRIYNEPTEEQIRDITAMTTCPDCG
                     APVRRRSF"
     gene            4398..5147
                     /locus_tag="PK196_06365"
     CDS             4398..5147
                     /locus_tag="PK196_06365"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HON41076.1"
                     /translation="MPGTRYWEIDLARGVAVVTMIIFHSAFDLNFFGILPLDVSGGFL
                     WLLARLTASTFIFLVGLSFTISYARARRRLTARGLALRYARRGLAIFGCGMVITGVTW
                     LFLPSVCIVFGILHFIGLSILLAPLFVRLDARRLIVASVACFIAGYAVTLVNGPWHLL
                     WLGIRPASFASLDYVPLFPWFGIVLAGMACGHTFYPDGRRGFRLGDPGPAVLRPFSFL
                     GRHSLLIYFLHQPVILFMIAVLAPGAVRFFG"
     assembly_gap    5298..5397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5398..6932)
                     /gene="ade"
                     /locus_tag="PK196_06370"
     CDS             complement(<5398..6932)
                     /gene="ade"
                     /locus_tag="PK196_06370"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HON41077.1"
                     /translation="MDFAVKDGCIIGIGDYQAERDHDLSGTYVVPGLIDAHVHIESSL
                     LAPAEYARLVLSHGTTTVIADPHEIANVCGTAGIEYMLASGAQTPLDILIMLPSCVPA
                     TPLDMGGARLTASDLARFRGRERVIGLAEVMNVPGVLAGDEDLRAKMDLFEVIDGHAP
                     SLSGKDLNAYIYAGVQSDHESTTLAEARERLLRGIYVMIREGSTEQNLRGLLPLVDRC
                     TAPRCCFATDDRHAGTLVRDGHIDDCIRKAVSYGLDVELALRMATLSAAERFRLHDRG
                     AIAPGRLADFCVIDDPDRFCVLRTFKRGVEVLDPGYRPPEVLKTPMQARVPGRDEIRI
                     TGRGVARVIGIVPGHITTRCLHSQVDAAAIPDVERDILKAVVTDRYRGSGSGVGLVHG
                     FGLEEGAIAGSVSHDSHNIVAVGVDDADIIRAIAEVVRLDGGLVVVSGNEVTTLPLEC
                     AGLISALPCDEVVRRLAALDEHAGRLGAIENPFMYLSFLALTVIPELRVTERGVFDVG
                     AFS"
     gene            complement(7031..7447)
                     /locus_tag="PK196_06375"
     CDS             complement(7031..7447)
                     /locus_tag="PK196_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41078.1"
                     /translation="MDEGVSGEPAEHRFSRGISARYTGILLLMAALAVLAASAGCLAT
                     AIGEVTYDGEALQVAVENSGEPVENAVLQVKIMEVDLFEQQKVFSQARHINLDSGENN
                     YSIEVDLQPGTYRAFLNIFIGDERGASVIRDLEVAV"
BASE COUNT         1363 a         2279 c         2265 g         1485 t
ORIGIN      
        1 tcgagagagt ggtcagccgc aaaagacgct cagcgcggcg gtgatttttg ggcatcaaac
       61 cgtgagcaaa ccggttcact tggctccatg cgggcgcctg tgaaaaagag tggttttttt
      121 agagggtctc cagccgtttg ttcaccatat cccagttcac gacgctccag aacgcatcga
      181 tgaactgccc ccggttgttc cggtaatcga cgtagtaggc gtgctcccag acatcgagca
      241 ccatcaggat cttgaatgag gggtagacgt tgatgttgtg cttctcgatc tgcattatga
      301 tgggccgatc ggtcatgggg cagcaggcga gcgcggccca gcctgaaccc tcgacgcttg
      361 cggccgcttt tgtaaactcg gccttgaacc ggtcgaagga gccccattct ttctctataa
      421 gatcagcaag ggcgccgccg ggtgtcccgc cgcctcccct gccggccggg gccatcgtgg
      481 gccagaagag ggtgtgcagg acatgaccgc cgatgttgaa ggagagttct tttagaagcg
      541 ctttcatgtc aacttcggcg ccttcctgcc gcgctttctc cagcctctca atgttggcgt
      601 tcgctcccgt cacgtagccc tggtggtgtc tgtcgtggtg gatggagagt tgctcctttg
      661 agatctgcgg ttcgagtgcg tcggccgcat agggcagcgg cgggagttca tacttcttga
      721 acggtctggt tgtcatcttc tctacagtca tggttatcga tcgttagatg gaggcggatc
      781 tatttcatgg tgtcggtgct ctcgctccag ggggctggtt gctcttttcc gctggatggc
      841 gcggcccgga tccataccgc taacttaacc tggatgccct ctcgctaatt cgccggagat
      901 ccggatggcg tgtccagaca tctccgcatg gctccacaag cctgcagcca ccagggtaaa
      961 catcgtcacg caccttccgg gtaatgggag atcaatcatc ggcccgggcc caacaaagcc
     1021 tatatagcag cggcgttgag tcttgagaga gtgaactctc ccgggagacc cgccgccggc
     1081 gatgcctaca taacacttca cggacgctcg acatgggcgc ttgtaaacgc ataccacgca
     1141 gcagtgcggg agagagggct gcgccccgaa cgggtcgtta tcgtcaccga agagccgtat
     1201 gcagaggcgg catccaccgc ggaggcagcg atcgggatgg tctcggaggg gtatggcctc
     1261 tccccggaga ttcaggtgga ggttctgccg gaggcagact tcgtcggcgc cgggcagatg
     1321 atccgctctc tcgcgggcgg cttcatccag caggggtatg acgttgcgat agacataacc
     1381 tctggcagga aggtcacggt cgccggggcg ttaatcgcgc tctcggttgc ggggcttgat
     1441 atccggcaca tctactacct ggccatgaag accgccgatg acatagcaaa gccctacatg
     1501 atgatcccgc accaggttca gaggatccac gaccttatcg aggactcagc ggcactttca
     1561 gcatgaacga ccttataata agggaggatg aactccaggt tctcatcaac tctctcgaag
     1621 aggttcatgt ctcatacccg ctctacgcgg gtgagatcat tgttgcgtgc ccggaaggaa
     1681 ccgggttctc gctccagatg cctgcggcgc gcgagacgtt ccgcgactgg gtcgccgcat
     1741 acgaaccgat tgcagcggag ctcccttcgt atgccgatct ccaggagtgc atgtttgcag
     1801 ccggcatcgc ccgctacaca aaccaggccg ccttcgatgc gatgcttgta tcctacagga
     1861 acctcaagaa atctgtctac ttcgggctgg atacaaacct cttctaccac gggtttgcat
     1921 cgaacaaccc cgggatcgac gcctccacct accttatcgt cgagaccgtc cgcgacgaga
     1981 tctcctacgc cgtcaaccgg aagtattcgg cggcggcaat cgaggagatg acggcactgg
     2041 cgcctgagtg ccgggattac ctcgcagacc tcgagaacaa acggatgaaa cgctcacgga
     2101 aagccgccta tcttgcactg aaagagtacc gctccatccg tgaccgggca acggaggtcc
     2161 cggcgcccgg gccgcacacc caccttgcag aggagaacga tcgcaacatc gtccgggcgc
     2221 tacggaagtt cgaggaggaa cgctacgccc tccccgttct cctgaccgcc gacatctaca
     2281 tggtcgacct ctgcatgaca gaggggcttg aatatttcta ctttgaccgt ccatacagac
     2341 ttgaggcaac aacctgcacg gcaacggcgt tccggcggct gctcttcaac cttgctgccg
     2401 tcttcgggtt tgtggactgc agcgggttca caatcttcgg tgagtacggc gggaaggcta
     2461 acgacctcga cgaactgaag atcaggttcc gggacgagga tatctaccgt gaatgcaggc
     2521 gggaacttgg tatatgcagg agacttgcag aactcggaat tacgcggtag aggctgtcct
     2581 ctgcgatatg gacaacaccc tctttgactt tgttcgagca aagcgggagg cgtgccgcag
     2641 tgtgatcgac tacctgggga gaggcgaccc cgggacgctc ttctcgcagt ttcttcgggg
     2701 ggttcacggg tttgaggacc atgagaacat aagtgactat ctcaacgatc tcggggtcta
     2761 cgatggggag acgtttgagg tctgctgccg gatctacgag gatgtcaaac tcgacctggt
     2821 cgaggcctac ccgggcgtcg aggagacgct ccgggctctg caggagagcg ggattgggct
     2881 tgcggtcgtg accgacgcgg agtcgttcca ggcgcggcgg cggcttgaga agaccgggct
     2941 catagactac ttcgagacgg tggtgacacc ggacctctcg gggaggtgta aacccgagcc
     3001 ggactcactt ctctacgccc tccgccgact cggtgcgaag ccggaggagg cgatgatggt
     3061 cggtgacagc gccgcccgcg acatcgcggc cggacggcgg ctcgggatgg tgacagcgtt
     3121 tgccgcctac ggcgactggc gccagagccg cgtgacggat gtcggggcgg atatagtcct
     3181 ggcggagttt gcgcaactca gaagatacct cagcataccc gggaaagggt aggccccttc
     3241 agcagagcgg agagggcagg tgtggcccct ccaccgggag gcgggccccc tgcaccagga
     3301 tctgctggtc ggtctccgcc accttcccga ggatgatgcc ctttcccgcc ggtgtcatcg
     3361 caaagaccgg gatcgaggtc cctgcctgca ggagcgcgtt ctttgccgcc gcatcacgta
     3421 tatgccgcga tgcgcaggcg gtgatcaggt cggcggtcac ggccagcagg gcggcgtcct
     3481 cacgtgatac cccggtggtg tggacggcga ctatcacggt ctccgggaac cgttcccgga
     3541 tggccgccgc ctctgcggca tcggcggtgg tcacagcgac ccggcggtgg ccgaggcctc
     3601 tcgccagcgc gacaccggcg gcctggtcta tcgcggcggt cgcggtgtcc aggaccgcac
     3661 ctccgttctc ctgtatccgc gcgatcacct ccaggatggg ggatgtcttc acgagtccgg
     3721 acatccggcc gccgatcccc tgtatgaggg cggggttcgt cgcaacgacc gtccccgcgc
     3781 cgtcggagac tatcaccgcg gcgtccaggt ctcccctccg cacggcgctg ctaaggagct
     3841 ccgaggcccc gaagatgacg aagtccggcc cggcgagaac ctcccgcgcc ggggtgcaca
     3901 tgccaaatga cctgatccgc gcctcgatgt tgcgctttat cgcttccggc gtcatatcct
     3961 cgaccgggca ggcaaaccgc ttcgccagcg gacagtcccg gatcacgggt cttccgacct
     4021 cgacaacctg gccgttcctg accacgaccc ggcacctccc gatggcctct atgacgtgtt
     4081 cgtcatgctc gttcatacgg agatctgggt ctggcgtggg aataatgctg cctcagcggc
     4141 gggtcaaatc acttatcggg taaaacctct tcatcttgat gcaggaaaca tatgatgcct
     4201 ctgcagcagg aagggatggt gatgggacgt gggcaggatc tgtagcccgt tcatcgtgat
     4261 cgagtgcagc cggcagtgcg gcttctcccg gatctacaac gagccgacag aggagcagat
     4321 ccgcgatata accgcgatga cgacctgtcc tgactgcggc gcgcctgtcc gacggaggtc
     4381 gttctgaggg gggcgccatg ccggggacgc gctactggga gatcgatctc gcccgcgggg
     4441 ttgccgtcgt gactatgatc atctttcatt cggccttcga cctcaacttc ttcggcatcc
     4501 tgccgctgga tgtctccgga gggttcctct ggctcctcgc ccgcctgacc gcatcgacct
     4561 tcatcttcct ggtggggctc tcctttacaa tcagttatgc ccgtgccagg cgacgactca
     4621 cggcgcgcgg gctcgcgctc aggtatgccc gccgcggtct tgccatcttc ggttgcggta
     4681 tggtcataac cggcgtaacc tggctgttcc tcccctcggt ctgcatcgtc tttggtatcc
     4741 tgcacttcat cggtctctcg atcctgctcg caccgctctt tgtccggctg gacgcaagga
     4801 gactcatcgt tgcaagtgtg gcgtgtttca tcgcgggtta cgccgtaact ctcgtcaacg
     4861 gcccctggca cctgctctgg cttggcatcc gtccggcatc gtttgcaagc ctggactacg
     4921 tcccgctgtt tccatggttc ggcatcgtcc ttgccgggat ggcctgcggg catacattct
     4981 acccggatgg aagacgcggg tttcgcctcg gagacccggg gccggcggtc ttgcggccgt
     5041 tcagtttcct gggccggcac tcactcctca tctacttcct tcaccagccc gtgatcctgt
     5101 ttatgatcgc ggttctggca cccggggcgg tgaggttctt cgggtgaggg gacgcggaga
     5161 gcggcatgat gcggcgggca gtttttcccg aagaaccggg ggagatgtgg ttgaccgccg
     5221 cccggcagtg gcgattgccc ctggaaaacc gtgtactgga cgcagatgag gtctcttgtg
     5281 ttttccaggc gggaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc
     5401 gaaaacgccc cgacatcaaa gaccccccgc tccgtgaccc ggagttcggg gatgactgtc
     5461 agcgccagga acgagagata catgaacggg ttctcgatag cgccgagcct tcccgcatgc
     5521 tcatcgagcg ccgccagccg ccggaccacc tcatcgcagg ggagggcgga gatcagtccg
     5581 gcgcattcca ggggcagcgt cgtcacctcg tttccggaga cgaccacaag ccccccgtcg
     5641 agcctgacca cttcggcgat ggcacggata atatcggcgt catcgacccc gacagcgacg
     5701 atgttgtggg agtcgtggga gaccgacccg gcaatcgccc cctcctccag cccgaacccg
     5761 tggacaagcc ccacacctga cccgctccca cggtaacggt cggtgacgac cgctttgagg
     5821 atatcgcgct cgacatccgg gatcgcggca gcatcgacct gagagtggag gcagcgcgtc
     5881 gtgatatgcc cgggcacgat cccgatcacc ctggccacac cccggccggt tatccggatc
     5941 tcgtcacgcc ccggcacgcg ggcttgcatc ggagtcttga gaacctctgg cggtcgataa
     6001 ccgggatcga gcacctcgac gccgcgtttg aacgtccgca gaacgcagaa acggtcgggg
     6061 tcgtctatga cgcagaaatc ggcgagcctt ccgggtgcaa tcgcgccccg gtcatggagc
     6121 cggaaccttt cggcggcgga gagcgtcgcc atccggagcg ccaattcgac atcaagaccg
     6181 taagagaccg cttttctgat acagtcgtct atatgcccat cccggacaag cgtcccggca
     6241 tgccggtcgt cggttgcaaa acagcatcgc ggcgccgtgc acctgtcaac aagcggcagg
     6301 aggccgcgga ggttctgctc ggtcgacccc tccctgatca tgacgtagat cccccggagg
     6361 agtctctcac gcgcctcggc aagcgtcgtt gactcgtggt cactctggac gccggcatag
     6421 atgtaggcgt tcaggtcttt tccggagagg gacggtgcat gcccatcgat aacctcgaag
     6481 agatccatct ttgcccggag gtcctcatcg ccggccagga cgccggggac gttcatcacc
     6541 tcggcaagcc ctatgacgcg ctcccggccg cggaaccggg caaggtcgga ggccgtgagc
     6601 ctggcgccgc ccatatcgag cggtgttgcc gggacgcatg aggggagcat gatcaggata
     6661 tcgagcggtg tttgagcccc gctggcgagc atgtactcga tccccgccgt cccgcagacg
     6721 tttgctatct cgtgggggtc ggcgatcacc gttgttgtgc cgtgcgaaag gacgagccgg
     6781 gcatactcgg ccggtgcgag gagcgaactc tcgatgtgga cgtgggcgtc gatcagcccg
     6841 ggcaccacgt atgtgccgga gaggtcgtgg tcccgctccg cctggtagtc gccgatgcct
     6901 atgatgcagc cgtcttttac cgcaaagtcc acactctcaa atgtgcagga aaatgggttg
     6961 aagacctcag caccgctaaa gacggcatcc gcgggttcaa gcccacgtgc cgctgccagt
     7021 cccgtatagc tcagactgct acctccaggt cgcggatgac agacgcgccg cgctcatcac
     7081 ctatgaatat attcagaaag gccctgtatg tgccgggctg gaggtcaacc tcgatcgagt
     7141 agttattctc cccactatca aggttgatat gccgcgcctg ggagaagacc ttttgctgct
     7201 caaagaggtc tacttccatt atcttcacct ggagcacggc gttctcgaca ggctcaccgc
     7261 tgttctccac cgcaacctgc agggcctcgc cgtcgtaggt cacctccccg atcgccgtgg
     7321 caagacagcc ggccgaggcg gcgaggaccg caagggccgc catcagcagc aggatgcctg
     7381 tatatctggc tgatatcccc ctggagaacc ggtgttctgc cggttctcct gacacacctt
     7441 catccatttt attcacaatt ctggcttttg ttataaaaat attgtgaaaa aa
//
LOCUS       DAOSZX010000072         7417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_8483_length_7417_cov_18.189487, whole genome shotgun sequence.
ACCESSION   DAOSZX010000072 DAOSZX010000000
VERSION     DAOSZX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8483_length_7417_cov_18.189487"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1639
                     /locus_tag="PK196_06380"
     CDS             143..1639
                     /locus_tag="PK196_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HON41079.1"
                     /translation="MCARFLERFFKPKPHIVQSPPPPSIMHGAGAGVPEYRVKPYFIV
                     ASVEMGNTTTKCILTGTNLETGRTYIINKTVTMSRDVRPPKPGETVFGETLDGTKLTR
                     ESVTDLVRDTLIQSHKEARLSIKDDLDFVVRSTGVVAAMDSPDQVGDFVIALANGCLV
                     AGVPPRKMTPPMSIENLPPKLRKYSFADRVVFVGAVAGVLPPRGSTGVEMVANEMEGE
                     LAMAGIKEGAKWTPVDFRNPCISIDFGTTLDGRITSDVARDDPNPFARTIGNFCGLAG
                     AVPDAIVRGTGLVESRTGTALDVFGDHPVTASFGGRKRPIVDEYVNRCHEHIDIRVVP
                     SDRTRFGRVPISADVAEKSGVALIGCDVGVNGSEIPALEEIGHEIYENYGLGMLNEVI
                     DRVCARMALRLVDVAIGRGLVPPNSSIGFTGRAAISGRKPEYILEGITERQLYENPID
                     HVVFVDDGLARGAALMARCMNSLGKPKEPLGGVRGGPCIMARRIKIGK"
     gene            1653..1889
                     /locus_tag="PK196_06385"
     CDS             1653..1889
                     /locus_tag="PK196_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41080.1"
                     /translation="MAEEEQLYDLIIPPGVPRKMIYDVVEKFDVEVVQRQERLAFANM
                     EGDARDLLAFRGRKEVVEEVQDYLLARLREFIGE"
     gene            2144..2548
                     /locus_tag="PK196_06390"
     CDS             2144..2548
                     /locus_tag="PK196_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HON41081.1"
                     /translation="MVALSGEIKELFNRTKIMPLATASKSGVPNVAPMGSMRLAADDT
                     IWIMDNYMQKTLKNLKENPAAALSFHDPETKRCFQVKGSTEIRTSGPDYEKARDWMRS
                     KSDKYPAKSLIIMKITEVFECTPGKNAGKKVL"
     gene            complement(2561..3748)
                     /locus_tag="PK196_06395"
     CDS             complement(2561..3748)
                     /locus_tag="PK196_06395"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HON41082.1"
                     /translation="MDKGKVVLAFSGGLDTSICIPLLREHYGFDEIITVAVDVGQPPE
                     EIARATEKGRMLADKHYTIDAKEKFVSDCIFPSIRANGSYEGYPMGTALARPLIAAEV
                     VKVAEKEGASKVAHGCTGKGNDQLRFDFIFRTAGYDIIAPMREMNLTREWEIAYAQEH
                     NIPVSAVKEKPYSIDENCWSRSIEGGKLEDPAFHPPEDIYAWTVSPKDAPDAVEEISI
                     EFERGVPVALNGERLAGLDLIRELNRIAGRNGVGRNDMIEDRILGLKAREVYEHPAAT
                     VLLTAHTDLEHLVLTRSEIAFKRIVDDKWSELAYMGLVHEPLFHALTAFINTTQERLN
                     GTVDVGLYKGSATVLGRRSDAALYSSDLVSFDSTTLDQKHAVGYSNYFGLQARMLKNL
                     EKR"
     gene            4077..5336
                     /locus_tag="PK196_06400"
     CDS             4077..5336
                     /locus_tag="PK196_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HON41083.1"
                     /translation="MSPEESIILEQVTEEETAPAEPMIRVRSLTRVFGEEPEKAIELY
                     RAGRSKQEIFKETGSTVALIDISFDVMRGETFVLMGLSGSGKSTLLRCINRIIEPTSG
                     EVWIDGKDIVPMGEEELREVRRRKLGMIFQNFALLPHRTVLENVAFGLEIQGVPLEER
                     HRKAGEMLQMVGLGGYEDSMPDELSGGMKQRVGLARALTSDPDILLMDEAFSALDPLI
                     RREMQDELLDLQQRLGKTIVFVTHDLDEALKLGDRIALMKDGAIVQIGTPEEILTSPE
                     NEYVEKFVADVDLTRVLTAGVVMRRAEPVAQWTAGPRVALHLMEEHDISCIFVVSRQR
                     QLRGRVTLDDAVEAVRKGKRLEDILITDVPKVTPTTSLCDIISLVVKNPYPVAVVDDA
                     GRLVGSISRGAVLAALARKGEDTNATA"
     gene            5323..6168
                     /locus_tag="PK196_06405"
     CDS             5323..6168
                     /locus_tag="PK196_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HON41084.1"
                     /translation="MQLPKLPVDYGVEVLVDWIEHHFGWLLDAISAALGFLIGGLQDL
                     LLAIPVPILILIAAVVVWVVTRRNIKLAAVTAIGLILIWDLNLWSLSMMTLALVLVST
                     IAALALSIPLGIAAARSDTMNSALRPILDFMQTMPSFVYLIPAVIFFGLGNVPGAIAT
                     VVFAMPPALRLTNLGIRQVPTELIEVADAFGSTPWQKLVKVQVPVALPTIMAGVNQCI
                     MLALSMTVIASMIGAGGLGYQVLVGIQRVDVGMGFEAGLAIVIIAIILDRITQNLVPQ
                     RGERV"
     gene            6550..>7417
                     /locus_tag="PK196_06410"
     CDS             6550..>7417
                     /locus_tag="PK196_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HON41085.1"
                     /translation="MVRKMGSLLALLGIVLVLCLVSAGCTGTEEPKKTIRIGYVTWDC
                     AIASSNVMKQVFEEAGYDVEMIAVDAGPLFQGLASGEVDFTTTGWLPHTHAHYWEEYG
                     DQIDYVNENIPGAARIGLVVPAYVTIDSIEEMNDVVDKFGGRIVGIDPGAGIMTRTEQ
                     AIEEYDLDYELVASSSASMAAELSRAIESGEWVVVTGWSPHWKFGRWDLKFLDDPKGV
                     YGGAEDIVTLARKDLKTDDPEAYAILQRFRWTNEDIASVMTDIADGMPEEEAAKKWVE
                     NNRDKVDLWLGKA"
BASE COUNT         1580 a         2023 c         2286 g         1528 t
ORIGIN      
        1 gaggcggggc tggtcaagcc gcaaaagacg tctaagcgcg ctggtggttt ttcggggcat
       61 caaaccatga gcgaaccggt ttgccgggct ccatacagtc agatggaaag atatatgcag
      121 gcaggcggaa aaaacgatta ctatgtgtgc ccgctttctg gagcgctttt tcaaaccaaa
      181 gccgcatatc gtccagagcc cgccaccgcc gtcgatcatg cacggagcgg gcgcgggcgt
      241 tcccgaatat cgtgttaagc cttacttcat cgtggcatcc gttgagatgg gcaacaccac
      301 aacaaaatgt atccttaccg gcacaaacct ggagaccggc aggacgtata tcataaacaa
      361 gactgttacc atgagccggg atgtccggcc gccaaaaccg ggcgagaccg tattcgggga
      421 gaccctggac ggcacgaaac tgacgcggga gtcggttacg gacctggtgc gcgacaccct
      481 catccagagc cacaaagagg cacgcctgag catcaaagac gacctggact ttgttgtccg
      541 gagcaccggg gttgtggcgg cgatggactc ccccgaccag gtcggcgact ttgtcattgc
      601 ccttgcaaac ggctgcctgg tagcaggcgt cccacccaga aagatgacac cgccgatgtc
      661 gatcgagaac ctgcccccaa aactccggaa atactcgttt gccgaccggg tggtctttgt
      721 cggcgcggtg gccggcgtcc tgccgccgag ggggtccacc ggtgtcgaga tggtggcaaa
      781 cgagatggaa ggggaacttg cgatggcggg cataaaggag ggcgccaagt ggacgccggt
      841 tgatttccgg aacccctgta tatccatcga tttcggaacg acgctcgatg gccggatcac
      901 aagcgatgtc gcccgggatg acccgaaccc ctttgccagg acaattggaa acttctgtgg
      961 gcttgcggga gcggtgccgg acgcaatcgt tcggggcaca gggcttgtag agagtcggac
     1021 gggaacggct cttgacgtat tcggtgacca ccctgttacg gcgtcatttg gcggcaggaa
     1081 gcgccccatc gtcgacgaat acgtaaaccg ctgccacgag catatcgata tccgggtcgt
     1141 cccctctgac aggacaaggt ttggccgtgt cccgatcagt gctgatgtcg ccgagaagtc
     1201 cggtgttgcc ctcatcgggt gtgacgtggg cgttaacggc agcgagatcc cggcgctcga
     1261 ggagataggg cacgagatct acgagaacta cggtctgggg atgttgaacg aggtgatcga
     1321 ccgggtctgt gccaggatgg cgctccggct tgtcgatgtt gccatcggga gggggctggt
     1381 cccgccgaac tcatcgatcg ggttcaccgg gcgggcagcg atctcggggc gaaaacccga
     1441 gtacatcctg gagggcataa cggagcggca actctatgag aacccgatcg accacgtggt
     1501 cttcgtcgac gacggtcttg ccaggggggc ggcactgatg gcgcggtgca tgaactcgct
     1561 cggaaaacca aaggagccgc ttggcggggt gcggggaggg ccatgcatta tggcccgccg
     1621 tataaaaata ggaaagtagg tactggtaaa ccatggctga agaagaacaa ctctatgatc
     1681 tgatcatccc gccgggcgtg ccccggaaga tgatctacga tgttgtcgag aagttcgacg
     1741 ttgaggttgt ccagcggcag gagagactgg cgtttgcgaa catggaaggg gacgcacgcg
     1801 acctgcttgc gttccgcggc aggaaagagg ttgtggagga ggtccaggac tacctccttg
     1861 cgcgactgcg ggagttcatc ggggagtaat ccatctttcc attgcttttt tgagtcggta
     1921 tgccgttgta gtataagagc ggcaggactg gcgtttttag atcccccggt agtggatggg
     1981 cggaggagcc tcctcatctc ctgcatcccc ccggttaagc atccttttcg tcatcactcc
     2041 cgccgctcgc cgtatcgctt atataccggt ggcacggata acctgactgc ctgacgggcg
     2101 gccccgcgcc gtatctcgtt tcaggcagga ggtggaaacc cgtatggttg cgctctcagg
     2161 tgagattaaa gagttattta acagaacaaa aattatgccg cttgccaccg cctcaaagag
     2221 cggtgtcccc aacgtggcgc cgatgggatc gatgcggctc gccgccgacg acaccatctg
     2281 gatcatggac aactacatgc aaaagaccct caagaacctc aaggagaacc cggctgccgc
     2341 cctctccttc catgacccgg agacaaaacg ctgttttcag gttaagggca gcacagagat
     2401 caggacgtca ggtcccgatt acgagaaggc ccgggactgg atgagatcaa agagtgataa
     2461 atacccggca aaatcgctca tcatcatgaa gatcaccgag gtttttgagt gcacgccggg
     2521 aaaaaacgcg gggaagaagg tgctgtaggc accccctccc tcatcgcttc tccaggtttt
     2581 tgagcatgcg cgcctggaga ccgaagtagt tagagtaacc gacggcgtgc ttctggtcaa
     2641 gcgtcgtcga gtcaaaggat acaaggtcgc tggagtagag cgccgcatcc gacctccggc
     2701 caagcaccgt tgcgctgcct ttatagagcc cgacgtccac cgtgccgttg agccgttcct
     2761 gagtggtgtt gatgaacgcc gtcagcgcgt ggaagagcgg ttcgtgcaca agccccatgt
     2821 aggcgagttc cgaccactta tcgtcgacga tccgcttgaa cgctatctct gaacgggtca
     2881 gcacaaggtg ctcgaggtca gtgtgcgcgg tgaggaggac ggttgcggcc gggtgctcgt
     2941 agacctcacg ggccttcagc cccaggatcc ggtcctcgat catgtcgttg cggccgacac
     3001 cgttccttcc tgcgatccgg ttcagttccc ggatcaggtc aagccccgca agcctctctc
     3061 cgttgagggc gaccgggaca ccccgctcaa actctatgct gatctcttcc accgcatccg
     3121 gggcatcttt gggggatacc gtccaggcgt agatatcctc cggcgggtgg aaggccgggt
     3181 cctccagttt accgccctcg atgctcctgc tccagcagtt ctcatcgatg ctgtatggtt
     3241 tttccttcac cgcagagaca gggatgttgt gctcctgagc gtaggcgatc tcccactccc
     3301 gtgtcaggtt catctcccgc atcggcgcga tgatatcgta gcccgctgtc ctgaagatga
     3361 aatcaaaccg gagctggtcg tttcctttcc cggtgcagcc gtgcgcgacc tttgatgccc
     3421 cctccttctc ggcgaccttt acaacctctg cggcgatcag cggtcgggcg agcgccgtgc
     3481 ccatggggta gccctcgtat gagccgtttg ccctgattga ggggaagatg cagtccgata
     3541 caaacttctc ctttgcatcg atggtgtagt gtttatccgc aagcatccgc cctttctccg
     3601 ttgcccgggc gatctcctca gggggctggc cgacgtcgac cgcgacggtg atgatctcgt
     3661 caaacccgta atgttcgcga agcaggggta tacagatgga ggtgtcaaga ccccctgaaa
     3721 acgccagaac gacttttccc ttgtccatgg tggtggtctc cgtcttatag actctgtgga
     3781 ttgatctaca tggatatgag ttctcagcca ataaaagaaa cggtatggcc ggggagcagc
     3841 ggcctgccgg ttttgccggg agcgggcatt tgcggccgga tgctttggag cggcggatcc
     3901 tggaagaatg agcagtatgg gctgagtaaa ctgtaaagtg tccgtaaatt ctcagataaa
     3961 gattcgtgat ctctgccagg ggtgaaaccg ccctggagcg tcctgaaaat caaaactcga
     4021 gactaaaatg aagatttttc aaaacccgaa attataaacc agatgaaata gaaattgtga
     4081 gcccggagga gtccatcatt ttagagcagg taactgaaga agagacagca cccgcggaac
     4141 cgatgatccg tgtcaggagc ctgacccggg tattcgggga ggagccagaa aaggccatag
     4201 aactttatcg tgcgggccgc tcaaaacagg agatctttaa agagacgggc tccacggtgg
     4261 ctctcatcga tatctcgttc gatgtaatgc ggggtgagac atttgtcctg atgggcctct
     4321 ccgggagcgg gaaatcgacg ctcctgcggt gcatcaaccg gatcatcgag cccacgtcgg
     4381 gcgaggtatg gatcgacggc aaagatatcg tccccatggg cgaggaggaa ctccgggagg
     4441 tcaggagacg caaactgggc atgatattcc agaacttcgc gctcctgccg caccggacgg
     4501 ttcttgagaa cgtcgccttc ggcctcgaga tccagggcgt tcccctggag gagcggcaca
     4561 gaaaggccgg agagatgctc cagatggtgg ggcttggcgg ctacgaggac agcatgccgg
     4621 atgaactgtc tggagggatg aaacagcggg tcgggcttgc ccgtgcgctc acaagcgacc
     4681 cggatatcct cctgatggat gaggctttca gcgcgctcga ccccctgata cgccgggaga
     4741 tgcaggacga actcctggat cttcagcagc ggctcggcaa gacgatcgtc tttgtcaccc
     4801 acgatctcga cgaagccctg aaactgggtg acaggatagc gctgatgaag gatggcgcga
     4861 tcgtgcagat cggcactccg gaggagatcc tgaccagccc cgagaacgag tatgtggaga
     4921 agtttgttgc ggatgtcgac ctgaccaggg ttcttacggc aggcgtcgtg atgcgccggg
     4981 ccgaacccgt cgcccagtgg acggcggggc cgcgggttgc cctccacctg atggaggagc
     5041 acgatatatc ctgcatcttt gttgtcagcc gtcagcggca gctccgcggc agggtgacac
     5101 ttgacgatgc cgttgaggct gtcaggaagg gaaaacgact tgaagatatc ctcatcacag
     5161 atgttccgaa ggtgactccc accacatctc tgtgcgatat catatcactt gtcgttaaga
     5221 acccataccc tgttgcggtg gtggacgatg ccggcaggct ggtgggcagc atatcgcgcg
     5281 gcgccgtact tgccgccctg gcacgaaagg gggaggatac gaatgcaact gcctaaactg
     5341 cccgtggact acggagtcga ggtgctggtg gactggatag agcaccactt cggctggctg
     5401 cttgatgcca tcagcgcggc gcttgggttc ctgatcgggg ggctgcagga tctgctgctt
     5461 gccataccgg taccaatcct gatactcatt gcggccgtgg tggtctgggt tgtcacccgg
     5521 cgcaacataa aactggcggc agtcaccgca atcgggctta tcctgatctg ggatctcaac
     5581 ctctggagcc tctccatgat gacgctggcg cttgttctgg tctcgacgat agccgcgctg
     5641 gcgctatcga tccctctcgg gatcgctgct gcaaggagcg ataccatgaa ctccgcgtta
     5701 aggccgatcc tcgacttcat gcagaccatg ccctcgtttg tctacctcat ccctgccgtg
     5761 atcttctttg gtcttggaaa cgtgccgggc gccatcgcaa cggttgtgtt cgcgatgcca
     5821 ccggccctgc gtctcaccaa tcttgggatc aggcaggttc cgaccgagtt gatcgaggtt
     5881 gcggatgctt tcgggtccac gccctggcag aaactcgtca aggtgcaggt ccctgttgcg
     5941 cttccaacga tcatggcagg ggttaaccag tgtatcatgc ttgctctctc aatgaccgtc
     6001 atcgcatcga tgatcggtgc cgggggtctt ggctaccagg tgcttgtggg gatccagcgg
     6061 gtggatgtcg gtatggggtt cgaagcgggg ctcgccatcg tgatcatcgc gatcatcctc
     6121 gaccggatca cccagaacct ggtgccacaa cgtggtgaga gggtgtaagc aggtctcgaa
     6181 aagcccatct taccccgcat tctctcttct cttgctgaga tcctggcgat gccgggatga
     6241 cgcttcggtg tgcgccccag gagcgatttc aggggtgcac accacttcaa atagggtttc
     6301 agaaacgctc cgttcttaaa aaggaaccag aaaaacctaa ataaggtgct cttcataatt
     6361 tggaggggtc aaatactggc cgttttgctg tggggtgcaa aagcgccagg agtggcagat
     6421 gctgccacct ctgctctatg ccgggatcgc agatcctgga gggctggaaa gacccggaaa
     6481 ccccacacaa cctggatcta taatgcatgc cgccgtgttg tgcggtaaaa aactgagaag
     6541 gtaaattata tggttcgaaa gatgggaagc ctgcttgcac tgctgggtat tgttctggtg
     6601 ctctgtcttg tatctgccgg ctgcaccgga acagaggagc cgaaaaagac tattcgcatc
     6661 ggttacgtca cctgggactg cgccatcgcg agcagcaacg tgatgaaaca ggtctttgag
     6721 gaggcaggct acgatgttga gatgatcgct gttgacgcgg gcccgctatt ccaggggctt
     6781 gccagcggtg aagttgattt cacgacgacc ggatggctcc cgcacaccca tgcgcactac
     6841 tgggaggagt atggcgacca gatcgactac gtcaacgaga acatacccgg tgcggcccgc
     6901 atagggcttg tggttccggc ctatgtgacc atagactcga tcgaggagat gaacgatgtc
     6961 gtcgataagt tcggtggtcg gatagtcggg atcgaccccg gtgcgggcat catgacccgg
     7021 actgagcagg ccatcgagga gtacgacctc gactacgaac tggtcgcaag cagcagtgcc
     7081 agcatggccg ccgaactgag ccgtgcaatc gagagcggtg agtgggttgt ggtgacgggc
     7141 tggtcgccgc actggaagtt cggtcgctgg gacctcaagt tccttgacga cccgaaaggt
     7201 gtctatggcg gcgccgagga tattgtgacc ctggcgcgca aggatctgaa gaccgacgac
     7261 cctgaggctt atgcaatcct ccagcggttc cgctggacca atgaggatat cgcctcggtc
     7321 atgaccgata tcgcggatgg catgccagaa gaggaagccg caaagaaatg ggtcgagaac
     7381 aaccgcgata aggtcgatct ctggctcggg aaagcat
//
LOCUS       DAOSZX010000073         7253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_8729_length_7253_cov_20.440817, whole genome shotgun sequence.
ACCESSION   DAOSZX010000073 DAOSZX010000000
VERSION     DAOSZX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8729_length_7253_cov_20.440817"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /gene="glmM"
                     /locus_tag="PK196_06415"
     CDS             complement(<1..1358)
                     /gene="glmM"
                     /locus_tag="PK196_06415"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HON41086.1"
                     /translation="MQKTKRMFGTNGVRGVTGETMTPVLVYKIGAALGSMRKGTIAVG
                     RDTRTSGEALSHALKAGLLTTGCDVLDLGILPTPALQYIIRTGRFSGGAMITASHNPP
                     EYNGVKVIEPDGTEMSDEETINLEERFFAEDFDLAAWDAVGRESAAPDRLEDYIEAVA
                     SHFPPGIGEGMTVVVDPGSGPAALTTPIILSRLGCRVHTINARLDGTFPGRMPEPSPE
                     GLQPLAEMVVATGADFGVAHDGDADRAVFIDDRGRYVEENCEFGLIEDYICSRKRGGI
                     IVTPVATSSLIREIAERYGCSVDYTPVGSIYVARRMIELIAAGKDVVFGGEGNGGLIY
                     PDHQFCRDGGMTAAMMVAVLASHKGRRLSGIIDELPAYNLIKEKYRTTEPHAVVRAVE
                     EAFSGERIEKIDGIKILRENAWALVRPSGTEPMVRIMVEAKDPRVADALYQEIAQVLA
                     KRV"
     gene            complement(1367..2074)
                     /gene="mobB"
                     /locus_tag="PK196_06420"
     CDS             complement(1367..2074)
                     /gene="mobB"
                     /locus_tag="PK196_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HON41087.1"
                     /translation="MKVIQVVGRSNAGKTTFIKSLIVALSPHGPVGVVKHLGHHGFFI
                     EPGKDTTAYYESNAAISCGVDEEKSVFISQETDLNSALDTLCNAGVRYAILEGFKARV
                     FPRIVIGDLECEKTLLRDPSVGEVIGSLDDFEDYYTMEGLVRELRHDCGVSHAGSILT
                     FSGIIREQMGEKRIDDPAERVQGEIESLPGILGARVYHRRDIVHIAILAEEGQEAFAA
                     AVSAIERLRQELKLPEE"
     gene            complement(2071..2643)
                     /locus_tag="PK196_06425"
     CDS             complement(2071..2643)
                     /locus_tag="PK196_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HON41088.1"
                     /translation="MDDPENLKDPNRYQKFKKVDGATYKRVNQFLRKHTYITAREWAI
                     ARLCADFKTSSGSEMTFIGENLPKLCPFMVDTYTPQAVNQARSSFKKKVKKSGATFFY
                     GAMCGFFTAEELDEILFEASEVARFLLEVEGTSLNLDDEIDIEDRIAEVMRGVAEAAS
                     VILKTRPGQEGEKNQEGDRGAQNDEEEEMR"
     gene            3972..4889
                     /locus_tag="PK196_06430"
     CDS             3972..4889
                     /locus_tag="PK196_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HON41089.1"
                     /translation="MENVSFSEWIDRFSGYLRMRNYSPRTIEKYIQTIRRFARYAWVR
                     ENLDPDGAGFDEAMLDNAPLDAGVNVSTALVTDFFSYLAERRDYRPRTLHRMISTLSS
                     FYRYLYVQGAVAADPMIGVERPRIKNQELKYLKHSQVIRLINTIENERDRLIVRLIYA
                     TGVRVSELCSINIEDIDFEEHTIRVRGKGDKIRTVFVDDETLEEIERFIGNKIAGPLF
                     PGQQGKHLSPRTVQHIFRQYAPPGITPHKIRHSYASELYRRSKNLRVVQENLGHSSIK
                     TTEIYLHTDIDERKRVYQQYFPLSNGGDE"
     gene            5009..5944
                     /locus_tag="PK196_06435"
     CDS             5009..5944
                     /locus_tag="PK196_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON41090.1"
                     /translation="MRYIVTGGAGFIGSGLADRLAADGHEVLIIDNLSSGRRENIEHL
                     LDNPCVTFVEGSVTNLDLLLDLSLGADGIFHQAAIASVPRSVADPLETNEVNVTGTLN
                     LLWAAKECGVPAVVSASTSAIYGDDPVFPKEESMPPAPLSPYAVSKLAGEYYGRVFSD
                     LYGVRTVFLRYFNVYGPRQDPNSDYAAVIPKFITRLIEDRPPIIYGDGEQTRDFIFVS
                     DVVRANIMAMESSASGVFNIAGGSRINLNQLASILSAITGVNRRPVYDQPRPGDVRDS
                     VAAISRAREAFGFAPGYTLEEGLRETVAWFRKMVG"
     gene            6055..>7253
                     /locus_tag="PK196_06440"
     CDS             6055..>7253
                     /locus_tag="PK196_06440"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HON41091.1"
                     /translation="MKTIILAGGSGTRLFPLSREHYPKQFIPLLDDESLFQNTVKRAL
                     LLSTPQEITIVTNAEHRFLVRDHLAAIGCDCRVLVEPVGRNTLPAIYYGVKEITRENG
                     PDTVAVLPSDHLITATGEFQEAFRRAERFAKDYLVVFGVRPTTPHTGYGYIRPGEPLG
                     DGGARVDAFVEKPDLETARSYVADGYLWNSGMFCFDAGLFLSECEVCAPEVVRAFEQP
                     LEDAYNATPALSIDYGVMEKTRRAAVVPLESDWSDVGNFDAVYAALPKNGDGNAVRCE
                     HIGIDSSGNLIIADSLVATVGVHDIAIVETKDAILVAARDEAQRVGEIVKALRDRGDT
                     RALFHTQVHRPWGSYTNLEEGRSYKIKRVTVPPKRRLSLQMHHHRSEHWVVVTGCAEV
                     TIGDKTFL"
BASE COUNT         1435 a         2149 c         2051 g         1618 t
ORIGIN      
        1 actctcttcg ccagaacctg cgcgatctcc tggtagagag cgtctgcaac gcgcggatcc
       61 tttgcctcga ccattatcct gaccatcggt tctgtgccag atggccgcac cagcgcccac
      121 gcgttctctc tcaggatctt tatcccgtcg atcttctcga tcctctcccc agagaatgcc
      181 tcctcgacgg cgcgaacgac tgcgtggggc tccgttgtgc ggtacttctc cttgatcagg
      241 ttgtatgcag ggagttcgtc gattatccct gagagtcttc ttcctttatg actggctaga
      301 actgcgacca tcatggcggc ggtcataccg ccgtcccggc agaactggtg atcagggtag
      361 atcagacctc cgtttccctc gcccccgaag acgacatcct ttccggcggc tatcagttcg
      421 atcatccgcc ttgcaacata gatgctcccc accggtgtgt aatcgacgct gcaaccgtac
      481 ctctctgcga tctccctgat caggctggac gtggcaaccg gggtgacgat gatcccgcct
      541 ctcttcctcg agcagatgta atcctcgatc agcccgaact cacagttctc ctcgacgtat
      601 cgccctctgt cgtcgataaa taccgcccgg tcggcatccc cgtcgtgggc gaccccgaaa
      661 tcggcgccgg tggcaacgac catctctgcg agtggctgca gcccttcagg gctcggttcg
      721 ggcattcggc ccgggaacgt gccatcgaga cgggcgttga tcgtatgaac cctgcagccc
      781 agtctggaga ggatgatcgg ggtggtgagc gccgccggcc ctgaacccgg atcgacgaca
      841 acggtcatcc cctccccgat gccgggagga aaatgggatg caacggcctc gatgtagtcc
      901 tcaagcctat cgggggcggc gctttccctg ccgaccgcgt cccaggcggc aagatcgaaa
      961 tcttcggcaa agaacctctc ctcgaggttg atggtctcct catcggacat ctcggtgccg
     1021 tcgggctcga tcacctttac accgttatac tctggcgggt tgtgcgatgc ggtgatcatg
     1081 gcgccgccgg agaaccggcc ggtccttatg atgtactgta gcgccggggt cgggaggatg
     1141 ccgagatcca gcacgtcaca gccagtcgtc aggagcccgg ccttgagggc gtggctcaga
     1201 gcctcgccgg acgtcctcgt gtccctgccg accgcgatcg tcccctttct catcgatcca
     1261 agcgcggcac cgatcttata gacgagcacg ggcgtcatcg tctctccggt cactccgcgc
     1321 acaccgtttg tcccaaacat gcgtttcgtc ttttgcatct catccatcac tcctcaggta
     1381 gtttcagttc ttgtctcagc cgttcgatgg cactcactgc cgctgcaaac gcctcctgcc
     1441 cctcttctgc aagtatagcg atatggacta tatctctcct gtgataaacc cgggcgccaa
     1501 ggatcccggg gaggctctcg atctctccct ggactctctc cgcagggtca tcaatgcgct
     1561 tctcgcccat ctgctccctg atgataccgc tgaacgtgag gattgacccc gcatgcgaga
     1621 ccccgcagtc gtgcctgagc tccctgacca gcccttccat ggtgtagtaa tcctcaaaat
     1681 cgtcgagcga gccgataacc tcgccgacag aggggtcgcg cagcagggtc ttctcgcact
     1741 caaggtcgcc tatcacgatc cgcgggaaca cccgggcctt gaacccctcc aggatggcat
     1801 acctgacacc ggcgttgcag agggtatcga gggcgctgtt gagatcggtc tcctgcgaga
     1861 tgaagaccga cttctcctcg tcgacgccgc aggatatagc agcatttgat tcatagtatg
     1921 ctgtggtgtc cttgccgggt tcgataaaga aaccgtgatg cccgaggtgt ttgaccacgc
     1981 ccacgggccc gtggggagag agcgccacaa tcaggctctt tataaacgtt gttttaccgg
     2041 cgtttgatct gccaactacc tgtatgacct tcatctcatc tcctcctctt catcgttctg
     2101 tgcacctcga tcaccttcct ggttcttctc gccttcctgg ccgggcctgg tcttcaggat
     2161 gaccgaggcc gcctcagcca cgccacgcat cacttccgcg atcctgtcct ctatatcgat
     2221 ctcgtcgtcg aggttcaggc ttgttccctc cacctccagc aggaaccggg caacctcgct
     2281 tgcttcaaat aggatctcgt cgagctcttc ggcggtgaaa aaaccgcaca tagcgccata
     2341 gaagaatgtg gcgccggatt tcttcacctt cttcttgaac gaactccgtg cctggttgac
     2401 cgcctgcggg gtgtaagtgt ctaccatgaa cgggcagagt ttggggaggt tctcgccgat
     2461 gaatgtcatc tccgagccgc tggaggtctt gaaatccgcg cagaggcgcg cgatagccca
     2521 ctcgcgggcg gtgatgtagg tgtgcttgcg caggaactga ttgacccgct tataggtggc
     2581 accgtctacc ttcttgaatt tctggtagcg gttagggtcc ttgaggtttt cagggtcatc
     2641 cattttacta tcctcttgtt ggttttgcta tttcaataag gctggtctgg acaggggggg
     2701 cgggagttct attcttccgc cggttcttcc agacaaacca gtgcagggga gagatcggga
     2761 cttcccgtgt cgggtctggc cgtggttcca tgagaaaacc tggttttcaa ccaggatatg
     2821 gtttatgaat ggttggttct cacattatgg agaccatcaa accgggtcgc tcgcggtttg
     2881 acacccacaa ataaccatcg cgcgaaacgt cttttgcagc tgacctcccg cccccttcga
     2941 gagggggcgg gcagtgtggg gtgatatctc cggtggccgt gccagggagc gaaacggaaa
     3001 ttttcggaag gattcgctga aaatctgtgt gagtgaagtc aacttctggc actgtattga
     3061 tctggaattg ccagtaagga ggtttcggtg agagcctgag aaataattct cttccaaaaa
     3121 atcccgtctg caccccccaa cctccacctc gcgctctttt tcccgcaccg cccctggccg
     3181 accatcatca cccggaccaa tgtatagcaa catatggttt atgaccctcc tcccaaggca
     3241 ctgttctgca gggttgatgg ggggcggcct ggcgcgttat atttggtttc tggcgttctg
     3301 ggggtgtttt ggttatacat gcttttcagc gctggaagac gccgggtttc aggtgctcga
     3361 accattgatg ccggcaaaat cctttgcagg ggggaactga cccgttactg tgttccacag
     3421 gcccagggct gcaaaacctg gcgggattat ccgtcatcaa tgagaagagg gcattttccc
     3481 gtgatcgtcg atcccggaca ccttcatatc gcgcttttaa gccaggatcg cgccctttgt
     3541 tgaatagtgg gttaccaggg gaagcgatct ggcgtccatg ctcatctctg gaggttgttt
     3601 atatacaagg acggtctgcg gctggggagg gtggcatgtt cacaccactt tttactttca
     3661 tgccgcgcgg tatggggttc atatacttac agggggatgt attcatcagg gttctgctcc
     3721 cctccgtcca cccgggggtg gggcggaccc gcatcatacc tcctaactcc gatccaatca
     3781 gcatctcccc aaaccttttt tgggaccccg gatagtccag ggcgtctggc tgtggatcgg
     3841 ggtttgcatc acagtttggg gagcatctta agatcgtgta tcaggtcacc cccccgccgg
     3901 gtatggataa gttgcgggga ataggcattt accgcaattt tgatatgctt tgatatttaa
     3961 ttcattctcc aatggaaaac gtctctttct cagaatggat tgatcggttc agtggttacc
     4021 tccggatgag gaactactcc cccagaacca tcgagaaata catacagacc atccggcgtt
     4081 ttgcgcgcta tgcctgggtc cgtgagaacc tggatcccga cggcgccggg ttcgatgagg
     4141 ccatgctcga caacgctccg ctggatgcag gcgtcaacgt atcaaccgcg cttgtaaccg
     4201 atttcttctc ataccttgcc gagaggcggg attaccggcc gcggacgctc caccggatga
     4261 tctcgacgct ctcctcgttc taccgctacc tctacgtcca gggcgcggtt gccgccgacc
     4321 ccatgatcgg ggtggaacgt ccccggatca agaatcagga gttgaaatac ctcaaacaca
     4381 gtcaggttat ccggctgata aacacgatcg agaacgagcg agaccggctg atagttcgcc
     4441 tgatatacgc aacgggtgtc cgtgtatcgg aactctgttc cataaatata gaggatatcg
     4501 actttgagga gcacacgatc cgggtcaggg gaaaagggga caagatccgg acggtattcg
     4561 tcgacgacga gacgcttgag gagatcgagc gttttatcgg caacaagatc gcagggccgc
     4621 tattcccggg tcagcaggga aagcacctct cgccacggac ggtccagcac atattcaggc
     4681 aatacgctcc tccagggata acgccgcata agatccgtca ttcctacgca agcgaactct
     4741 atcggcgttc aaaaaatctc cgtgtcgtgc aggagaacct ggggcactca tccatcaaga
     4801 caaccgagat ctacctgcat accgacatag acgaacgtaa acgcgtctat cagcagtatt
     4861 tcccgctctc aaacggggga gatgagtaga ggatataccc ttcctgatgg gatgataaac
     4921 catatgatgc tatatgtata caaggtggga gggcttatgg ccgcacaccg gcaacgctca
     4981 aatcaaagga acaccaatct gattttgcat gcgctacatc gtcaccggcg gggcggggtt
     5041 catcggttcc ggtctcgcgg atcggctggc agccgatggt catgaggttt tgataataga
     5101 taacctctct tccgggcggc gcgagaacat cgagcacctc ctcgacaacc cctgcgtgac
     5161 gtttgtggag gggagcgtca ccaacctgga tctgctcctg gatctctctc tcggtgcgga
     5221 cggaatattc catcaggcgg ccatcgcatc cgtcccccgc tctgttgcag acccgctgga
     5281 gacaaacgag gttaacgtga ccgggacgct gaaccttctg tgggccgcga aggagtgcgg
     5341 tgtgccggcg gtcgtgtccg cctccacatc ggcgatatac ggcgacgatc cggtatttcc
     5401 caaagaagag agcatgcctc ccgcgccact ctctccttac gctgtctcaa aactcgctgg
     5461 tgaatactac ggcagagtct tttccgatct ctacggtgtc aggacggtct tcctgcgcta
     5521 cttcaacgtc tatggaccca ggcaggaccc gaactccgat tacgccgcgg tgatcccgaa
     5581 gttcatcacc cggctgatcg aggacaggcc gccgataatc tacggcgacg gcgagcagac
     5641 acgggacttc atcttcgtct ccgatgtggt gcgggcaaac atcatggcga tggagagcag
     5701 cgcatctggg gtctttaaca tcgccggcgg gagcaggatc aacctcaatc aacttgcatc
     5761 catcctttcg gcgatcaccg gtgtaaaccg ccggccggtg tatgatcagc ctcgaccggg
     5821 agacgtgcgc gattcggtcg ccgcgatatc acgtgcccgg gaggcttttg gcttcgctcc
     5881 cggctacacc ctggaggagg gtctccggga gacggtggcc tggttccgga agatggtcgg
     5941 atgaggggat aatcggttct cgtctccctg ccccggacat acctcaggtc tttatctcca
     6001 gcaggcacaa aatagactac aacaaccggt gccgtgcggc acggggtcga tttcatgaag
     6061 actatcatac ttgcaggcgg gagcggcacc cgcctcttcc cgctctcacg agaacactac
     6121 ccaaaacagt tcatccctct gctcgacgat gagtccctct tccagaatac cgtgaagaga
     6181 gcgctcctcc tctcaacccc gcaggagatc acgatagtca ccaacgccga acaccggttc
     6241 ctggtcaggg accacctcgc cgccataggg tgtgactgcc gcgtcctggt ggaaccggtg
     6301 ggcaggaaca ccctcccggc gatctactac ggggttaagg agataacccg ggagaacggt
     6361 ccggataccg tcgcggtcct cccatcggat cacctgatca ccgcgacagg ggagttccag
     6421 gaggcgttcc gccgggcgga gcggtttgca aaggactacc ttgtggtctt cggggtgcgg
     6481 ccgaccacgc cgcacaccgg atacggctat atccgaccgg gagagcccct cggcgacggc
     6541 ggtgcgaggg tggatgcctt tgtcgagaag cccgatcttg agaccgccag gagttacgtc
     6601 gccgatggtt acctctggaa ctccgggatg ttctgttttg atgcagggct ctttctctcc
     6661 gagtgcgagg tctgtgcacc ggaggttgtc cgggcgtttg agcaaccgct ggaggatgcc
     6721 tacaacgcga cgcctgccct ctccatcgac tacggggtga tggagaagac gcggcgggcg
     6781 gcggttgtgc cgctcgaatc cgactggagc gatgttggga acttcgacgc agtatacgcc
     6841 gcccttccta agaacggcga tggtaacgct gtccggtgtg agcatatcgg gatcgactca
     6901 tccgggaacc tgataatcgc cgacagcctt gtggccaccg tcggtgtcca tgacattgcc
     6961 atcgttgaga cgaaggatgc aatcctggtt gccgcacgcg acgaggcgca gcgggtcggc
     7021 gagatcgtga aagccctccg cgataggggg gatacgcgtg cgctctttca tacgcaggtt
     7081 caccgcccct ggggttccta cacgaacctg gaggaggggc ggtcgtacaa gatcaagcgg
     7141 gtcaccgtcc cgccgaaacg gcggctatcg ctccagatgc accaccaccg gtcggagcac
     7201 tgggtggtgg tcaccggatg cgccgaggtg acgatcgggg ataagacctt cct
//
LOCUS       DAOSZX010000074         7217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_8790_length_7217_cov_21.645350, whole genome shotgun sequence.
ACCESSION   DAOSZX010000074 DAOSZX010000000
VERSION     DAOSZX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8790_length_7217_cov_21.645350"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..458)
                     /locus_tag="PK196_06445"
     tRNA            complement(386..458)
                     /locus_tag="PK196_06445"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Asn,seq:gtt)
     gene            complement(498..797)
                     /locus_tag="PK196_06450"
     CDS             complement(498..797)
                     /locus_tag="PK196_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41092.1"
                     /translation="MLSKIKSEIELVSRHLEVIRVVVEHQPIGIMRLSEILGLPYHRV
                     RYSLRILEQEGYIRASPAGAVATPLGADLLSSLDDEVDELIELLQAIRKNNSRNV"
     gene            complement(829..1062)
                     /locus_tag="PK196_06455"
     CDS             complement(829..1062)
                     /locus_tag="PK196_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41093.1"
                     /translation="MDEATKKAFKTRFATLTIMLNIIILCFAIGIFVLLRFAPEGTPG
                     LVIGLLLLAAGVIVSISFRKRYTRMKVWLSEQP"
     gene            1097..2251
                     /locus_tag="PK196_06460"
     CDS             1097..2251
                     /locus_tag="PK196_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HON41094.1"
                     /translation="MVSTETDRTNWLDSAIQDAGAAAYVAYGSSADANVRYLTRFRTT
                     DPVVYVHKPGERGLIVVSQMEYERAVRESTAAVVTRADVGFLDCIRAGESRWRATAHV
                     IADMAGGPLLAPSFFPFALARHLESFQPVILDEKGVVERMRAVKTPEEIQWIRGAQHV
                     TEAAMDRGIALIRSSVPKDGLLYRNGALLTSEAVRTEMHTTLLAHGCRGIDTIVSCGP
                     DTALPHHQGGGPLLEDEPIVIDIFPQDERTGYHADMTRTVVKGEPSPAIREMYDAVRA
                     AKERAISMLRAGAHGADLYREVVEFFRDRGYESNMRGFTHSLGHGVGLEVHEDPSLGP
                     EGGELCAGNVVTVEPGLYYPGIGGVRLEDMGMVTETGFDRFTQYEERLTV"
     gene            2248..3129
                     /gene="map"
                     /locus_tag="PK196_06465"
     CDS             2248..3129
                     /gene="map"
                     /locus_tag="PK196_06465"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HON41095.1"
                     /translation="MSMDDRVYDAYREAGALARKVLRRGAGLVKEGVSLLEMVETTEG
                     MVVEEGAQLAFPLNVSFNEAAAHDTAMPGDERTFARGDLIKVDLGVHVDGYIADTAMT
                     VDLGSHGPLVEASRAALDAAIAAVRPGIAAGDLGAIIQSKIEEHGYKPVANLTGHGLE
                     RYDLHAKPMIPNIAMNGGAILVEGMVFAIEPFATTGSGRVVEAPRVEIYKQVAARPAR
                     LPSAKRILETARPRRGLPFSRRWVPGDKVDLGLSTLVRTGVIHPFPVLHDIQGSFVSQ
                     AEHTLIVTADGCEVTTR"
     gene            3174..4460
                     /locus_tag="PK196_06470"
     CDS             3174..4460
                     /locus_tag="PK196_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON41096.1"
                     /translation="MTGDTGMRDLLAIMELLLTAEIFNRNENLDINDLHPLTREFFGV
                     GVGGSPEVKRPLIVSEGAIKKVLGVPETVCQAVRANPFVGYEEFGQRLRLLSLDTAAG
                     WFIKRGGKDRIRENPVLAFYFEGMDSIDLRYQDVRAKNPRFEDTKEYIEAKVSRVVGE
                     SEEMREARDLIIISAPAEVEYNLEKLVCTPRQEEVIKKIGVALEHRDFLKQHGIYEFG
                     RLLFVGPPGTGKTSLALAMSPELHMPVLEVRLAMVTSQYLGETSKNIDRIFELAKRLA
                     PCILFIDEFDFVAKTRVSDDHGAMKRAVNMLLKNIDQISFVRNGVLLIGATNHPRILD
                     EAAWRRFDEVVEFPLPDRKMRQAILEKVAATIECDCDFADIAARTEGFSGSDLRMMMK
                     EAMMSALMEGRHRIGPVDIEQGLLRVEERNLIRAGH"
     gene            4462..5187
                     /locus_tag="PK196_06475"
     CDS             4462..5187
                     /locus_tag="PK196_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON41097.1"
                     /translation="MRITLLGTGDAIGTPKVGCDCKNCREMVARGWSRLRTSFIIEIG
                     GKHILVESSPDLRQQLLRAGSPHIDAVIWSHGHYDHFIGFGEFYRVQKVPPVYAAPPV
                     MDYCSDYLHFLSFEKHAVPVFESFGLFGLTFTFFPVNHPPVYTCGLLIEHDDMTIAYT
                     SDTREDIPAESRNLLKKTDIDLLFVDAIAPEGYNISKHMNYVEAVRFAHDVGARDYRC
                     VHMSHLVPPGMPHAAYDMETFCW"
     gene            complement(5293..6027)
                     /locus_tag="PK196_06480"
     CDS             complement(5293..6027)
                     /locus_tag="PK196_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41098.1"
                     /translation="MEERRLTAVFVILMLMVSVCPGFASANPIADERGIDIEHGERTN
                     SVELLKASLNKREPVTITLGGPPSSKGGQNASYSDLENFKTATWDRIQERYLYPNGSV
                     IGFGYDVDGYLRVGLWNGPPPPENVSSVDSLHTMLDERAKSMGIESIPVKFTVFISPP
                     ELVLDGDRLNPPERTGPDAQSPENCPAKVTSLGVLTVALHEPEVIRLLENESIEVITF
                     SKGTYQKGVMTPRWSSAQRTLTPATA"
     gene            6784..>7217
                     /locus_tag="PK196_06485"
     CDS             6784..>7217
                     /locus_tag="PK196_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41099.1"
                     /translation="MKKERSWLKKVVSVPLLSLAILGCALLMAGCTSEGGPDSATPVP
                     TPPTFQPDILDKPIINAPAGKEGAPPFLFEFDTGNSSGFEEVRADRPGAPTLILGSNA
                     SATLPIIVSSEADTSVDVRVVRTDGLPYDVCVSYVPDSFTLR"
BASE COUNT         1510 a         2023 c         2155 g         1529 t
ORIGIN      
        1 gatagacctc gacgtttacg aacgtttcca ttcgagatcg ccgcgagaga ccggggcggg
       61 ttcgggttcg tgggtggttt acagttgttt gcgcaagggc gggcatgttg cgggtatagg
      121 agatcggccg ccccatcctg atgtctgtct gcgtctcccc ttgccaatga tgctctggca
      181 aaggttcccg tcacaaccct ggacgcggtt gtgcaccctg tagtgcagaa ttcttaaagg
      241 ggggcctccg atgcacaaac aatggcgaag gaacgttgat ccggatactt tgctccgata
      301 gactgcaggg ttgtacgggt ctacagggtg gtttctccag aaccaatgga ggtgccacaa
      361 caggcaaaga taactaaaag gaaagcgccg ccaacaggac tcgaacctgt gacatgctgg
      421 ttaacagcca gccactctac caactgagtt atggcggcac cggaacagtt gccctataca
      481 gtcttgactg aatcatatta aacgttacgg gaattattct tccggatggc ctgcaggagc
      541 tcaatcagtt cgtctacctc atcatcaagg ctggaaagaa gatccgcccc caggggtgtt
      601 gccaccgcac cggccggcga agcgcggatg tagccttcct gttccaggat ccgcagagag
      661 tagcgaaccc ggtggtaggg gagacccagg atttcagaga gcctcatgat tccgatgggc
      721 tgatgctcca cgaccacccg gataacctca aggtgccttg agacgagctc gatctcggat
      781 ttgatctttg acagcatggt tgccgcgcgg gcgaaatacc cacgcccctt atggttgttc
      841 gctcagccag accttcatcc gggtataccg cttccggaac gatatggaga cgatcactcc
      901 tgcagccagg agaagaaggc ctatcacaag cccgggcgtc ccttcgggcg caaaccggag
      961 gagtacaaag atgcctatcg cgaaacagag tatgatgatg ttgagcatga ttgtcagcgt
     1021 ggcgaacctg gtcttgaaag ccttcttcgt tgcctcatcc atcactcata cttgagcgcg
     1081 tggaataaaa gtacttgtgg tatccacaga gactgataga acgaactggc tggattcggc
     1141 catacaggat gctggtgcgg cggcatacgt ggcttacggc tcgtctgcag acgccaatgt
     1201 gcgttacctg acccggtttc gcaccaccga ccctgtggtg tatgtccaca aacccgggga
     1261 gcgcgggttg atcgtggtct cgcagatgga gtatgaacgt gcggtgcgcg aatcgacggc
     1321 agcggtggtc acccgtgccg acgtcgggtt cctcgactgc atcagggcag gggagtcccg
     1381 ctggcgggcg acagcgcacg tgatcgcaga catggccggc ggccctctcc tcgccccctc
     1441 tttcttcccg tttgccctcg ccaggcacct ggagtcgttt caaccggtca tactcgacga
     1501 gaaaggtgtt gttgagagga tgagggcggt caagacgccg gaagagatcc agtggatacg
     1561 cggtgcccag cacgttaccg aggctgcgat ggacaggggt atagcgctta tccgttcgtc
     1621 cgtcccgaaa gatggcctcc tttatcggaa tggcgcactc ctcacctccg aagccgtcag
     1681 aacggagatg cacaccactc tccttgctca tggctgccgt gggatcgata ccatcgtctc
     1741 ctgcggtcct gacaccgcgc ttccccacca ccagggcggg gggccgctcc tggaggatga
     1801 acccattgtc atagatatct tcccccagga cgaaagaacc ggttatcatg ccgacatgac
     1861 acgcacggtc gtgaaaggcg aaccatctcc ggcgatcagg gagatgtatg acgccgtcag
     1921 ggctgcaaaa gaacgtgcga tatcgatgct tcgtgccggt gcgcacggcg ccgacctgta
     1981 tcgtgaggtg gtggagttct tccgcgaccg cggatacgag agcaacatgc gggggttcac
     2041 ccacagcctc ggtcacggcg tagggcttga ggtgcacgaa gatccatccc taggtcccga
     2101 gggcggggaa ctttgcgcag gcaacgtggt caccgttgag cccgggctat actacccggg
     2161 catcggcggt gtccgccttg aggatatggg catggttaca gagaccgggt ttgaccggtt
     2221 cacccaatac gaagagaggc ttacagtatg agcatggatg acagagtcta cgacgcctac
     2281 cgggaagccg gggcgctggc aaggaaggtc ctccgcaggg gggcggggct cgtgaaagag
     2341 ggtgtctctc tccttgaaat ggttgaaacg acggagggaa tggtggttga ggagggtgca
     2401 caacttgcct tccccttaaa cgtctccttc aacgaggccg cggcacacga caccgccatg
     2461 ccgggcgatg aacggacctt tgccagggga gacctgatca aggttgatct cggtgtccac
     2521 gtcgacggct atatcgccga caccgcgatg accgtcgacc tcggcagtca cggcccgctc
     2581 gtcgaggcat cccgtgcggc cctcgatgca gcgatcgccg ccgttcgacc ggggattgcc
     2641 gccggtgacc tgggggcgat catccagtcg aagatcgagg agcatggcta caaaccggtt
     2701 gcaaacctca cagggcacgg tcttgaacgc tacgatctac acgcaaaacc catgatacct
     2761 aacattgcca tgaacggcgg ggcgatcctt gtggagggga tggtcttcgc aatcgaacct
     2821 tttgccacaa cgggatcggg gagggttgta gaggcgccca gggtggagat ctataaacag
     2881 gttgcggccc gccccgcacg attgccgtcg gccaaacgga tcctcgagac cgcccggccg
     2941 cggcgaggac tcccgttctc gcggcgctgg gtgccggggg acaaggtcga tctggggtta
     3001 tccacgctgg tgcgaaccgg ggtcatccac ccgttccctg ttctgcacga tatccagggt
     3061 tcgttcgtct ctcaggccga acataccctg atagttacgg cggatgggtg tgaggtcacc
     3121 acccgctgag ttatacttat tgatgtcgcg tgcaaatatg tcagttcaca gatatgaccg
     3181 gcgacactgg tatgcgcgat ctccttgcta tcatggaact cctgctgacc gctgagatat
     3241 tcaatcggaa cgagaacctc gacatcaacg atctccaccc gctcaccagg gagttttttg
     3301 gggtcggggt cggcgggagc cccgaggtga aaagacccct gatcgttagc gaaggggcga
     3361 tcaagaaggt tcttggcgtc ccggagaccg tctgccaggc tgttcgcgcc aaccccttcg
     3421 tcggttacga ggagttcggg cagcgcctcc gcctgctctc gctcgatacg gcagcgggat
     3481 ggttcatcaa aaggggcggg aaggatcgga tcagggagaa tcccgtgctt gcattctatt
     3541 ttgaagggat ggacagcatc gatctccgct accaggacgt tcgggcgaag aacccacggt
     3601 ttgaggatac aaaggagtac atcgaggcga aggtctcccg cgtcgtcggg gagagtgaag
     3661 agatgcggga ggcccgcgat ctcatcatca tcagcgctcc ggcggaggtt gagtacaatc
     3721 ttgaaaaact ggtctgcacc ccccgacagg aggaggtcat caagaagatc ggtgtcgccc
     3781 tcgagcaccg tgatttctta aagcagcatg ggatctatga gttcggccgc ctcctcttcg
     3841 tcggtccgcc tggcaccggg aagacgtcgc ttgcgcttgc gatgtccccg gaactccata
     3901 tgccggttct tgaggtccgc ctggcaatgg tcacctccca gtaccttggt gagacctcaa
     3961 agaacatcga ccggatcttt gaacttgcaa agaggctcgc tccctgtatt ctcttcattg
     4021 acgaattcga tttcgtcgca aagaccaggg tcagcgacga tcacggcgcg atgaagcgcg
     4081 cggtgaatat gctcttaaag aacatcgacc agatcagttt cgtgaggaat ggtgttcttc
     4141 tcatcggcgc aacaaaccat ccccgcatcc ttgacgaggc ggcctggcgg cggttcgacg
     4201 aggttgtgga gttcccgctt cctgaccgga agatgaggca ggcgatcctg gagaaggttg
     4261 ctgccacgat cgagtgcgac tgcgattttg cggacatcgc cgccaggacg gaggggttct
     4321 caggatccga tctgcggatg atgatgaagg aggctatgat gtcggcgctg atggaaggcc
     4381 ggcaccggat cggcccggtg gatatcgagc aggggcttct ccgggttgag gagcgcaacc
     4441 tcatccgggc cgggcattga gatgcggatc acgctcctcg ggaccgggga tgccatcggg
     4501 acgccgaagg tgggttgcga ctgcaagaac tgcagggaga tggtcgcaag agggtggtcg
     4561 cgactgcgga catccttcat catcgagatc ggaggcaagc acatccttgt tgagtcgtcg
     4621 ccggacctgc ggcagcagtt actccgggca ggttcacctc acatcgatgc agtcatctgg
     4681 tcgcacggtc attacgacca ttttatcggt ttcggagagt tttacagggt tcaaaaggtg
     4741 ccgccggtat acgcggcgcc gcctgtgatg gactactgct cggattacct ccactttctc
     4801 tcgtttgaga agcatgccgt cccggtcttt gagtcattcg gcctctttgg tctcaccttt
     4861 acgttctttc ctgtgaacca cccgccggtt tacacctgtg ggctcctcat cgagcatgat
     4921 gacatgacga tcgcctacac cagcgatacg cgggaggata taccggcaga gagccggaat
     4981 ctcctcaaga agaccgatat cgatctcctc ttcgtcgacg ccatcgctcc ggaggggtat
     5041 aatatcagca agcacatgaa ctacgtcgag gctgtaaggt tcgcccatga cgtcggggcg
     5101 cgggactatc ggtgcgtcca catgagccac ctggttccac cggggatgcc gcacgccgcg
     5161 tatgatatgg aaacgttctg ctggtgacta tccggctctc cccctgcagt ccgggatata
     5221 cgacggatag gtctcatagg cggcagaaac ccttcacgaa tcgttaacca gggaccacga
     5281 tcatcggagc ggtcatgcag tcgccggggt cagagtcctc tgggcggaag accacctggg
     5341 tgtcattacc cccttctgat aggttccttt tgagaacgtg atcacctcga tactctcgtt
     5401 ctccagcaac ctgatgacct ctggctcgtg aagcgccacg gtcaacacac cgagggaagt
     5461 gactttcgcc gggcagttct ccggcgattg tgcatcgggg ccggtacgtt ccggggggtt
     5521 caaccggtcg ccatcaagga ccaactccgg cggggagatg aataccgtga acttcaccgg
     5581 gatactctca atacccatgc tctttgcccg ctcatcaagc atcgtgtgca gtgaatccac
     5641 ggaagagacg ttctccggtg gaggcgggcc gttccagaga cccacccgga gatacccgtc
     5701 aacgtcatag ccaaaaccga tgacggaccc gttgggatag aggtagcgct cctgtatccg
     5761 gtcccatgtg gcggttttga agttctcgag gtcggaatat gatgcgtttt gcccgccctt
     5821 tgaggaggga ggtcctccga gtgtgatggt gacgggctct cgcttgttca ggctggcctt
     5881 caggagctca acgctgttcg ttcgttctcc atgttcgata tctatgccac gctcatcggc
     5941 aatcggattc gcggaagcaa accccgggca gaccgatacc atcagcatga gtataacaaa
     6001 cactgctgtg agccgccttt cctccatttc ttcacctcaa tcacgattga aatccgggcc
     6061 ctttgccggc atgacgcccc ccttcattgg catcactgcc atgaacataa gagtgcccgc
     6121 atcctcgtgt ttccgttcag atactctctt ctaggtgccc cttgaagaga ggagaggcac
     6181 atttacgtat gtaagttgtg tgcgcccctt ttgcctcggg gcatgcggga cgttcacccg
     6241 gacgtttggt aaaccggctg ggtgaacctc cgcaccgttg caggggtcgc ctgcggcacc
     6301 cacaatcgtc cacctggacg ttttgggatg aactgtttat gggacaaaca ccagatcgaa
     6361 cccgcctctt ctcaaaaaaa gcactccagg tgccctgaga tcaccgtaga tgatatcccc
     6421 tgtggctgat cggtctcgaa ggtaattctc ctcctggatg gtaaaagcag cgcggaatga
     6481 gcagaaaagg ctccagcagg tccggtgcag gggcgctctc gccgttcgcc gcgcgggtca
     6541 cccctggtgt caacggaatt atatttgggt agcggaacag aggatttcag agtggaaacg
     6601 cgtcccatta gggaagattg gacaaggatc ttagacggat cttggtatga accacgtacg
     6661 accttcaggt ggtataagtg cacaccggat gaacaatttg tctcaatttc tgcacaatgg
     6721 acagttgatg gtcgaattga gacgacgcac gtaggcggat cctctgtctg aggtgaagag
     6781 acgatgaaaa aggaaagaag ttggcttaaa aaggtagtgt cggtcccttt gctctctctt
     6841 gctattctgg ggtgtgccct tcttatggcc gggtgtacga gcgagggagg acctgacagt
     6901 gcgactccag taccgacccc tcccacgttc cagccagaca tcttggataa gccgatcatc
     6961 aacgctcctg ctggaaagga aggggctcct cctttcctct ttgagttcga tacggggaac
     7021 tcctcgggct tcgaggaagt gagggccgat cgcccgggag cgccgacact cattctggga
     7081 tcgaatgcat cggcgaccct cccgatcatc gtctcctcgg aggccgatac cagcgttgat
     7141 gtcagggtcg ttcggaccga cgggctgccc tatgatgtat gcgtctccta tgtcccggac
     7201 tccttcaccc ttcggat
//
LOCUS       DAOSZX010000075         7102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_8960_length_7102_cov_23.597985, whole genome shotgun sequence.
ACCESSION   DAOSZX010000075 DAOSZX010000000
VERSION     DAOSZX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_7102_cov_23.597985"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1625)
                     /locus_tag="PK196_06490"
     CDS             complement(153..1625)
                     /locus_tag="PK196_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HON41100.1"
                     /translation="MSLIVLGTASHVGKSVTVAALCRALYRRGIPVAPFKSQNMSLNS
                     YVTADGSEIGIAQAAQAFAAGIEPEADMNPILLKPKGEAVSQVVLLGRPYKDVQIRDY
                     YMETDKLLAEAVAAFERLQRRFGNVVVEGAGGAAEVNLYDRDIANIRLALALRLPVLL
                     VADIERGGVFAQVYGTLALLPEEIRSLVKGVIINKFRGDPALFESGVARLEELTGVPV
                     LGVVPYTDIRIPSEDSLSIYDGRRGVAGEAIRIAVIRLPRISNFTDFELLERHAVVEY
                     VTPGESLAGYDCIILPGTKNTVEDLNVLRGHGVEKELALSRERGVPIIGICGGYQILG
                     KAIIDSGIESGDDPAEYTGFGLLDVVTTFTGYRKTTVQVRRRATGPGPILQAMGEVEG
                     YEIHMGETERRGLPEAFAGEGASTPDGLVFGTYMHGLFQNPGAVNALLAYISKHRGLP
                     FEPITEADGGILGASAAYDDLARHFEAHIDMDAILKCFSG"
     gene            1673..3331
                     /locus_tag="PK196_06495"
     CDS             1673..3331
                     /locus_tag="PK196_06495"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HON41101.1"
                     /translation="MKGNMELTPAARLLLERRYLLPGESPEGLFWRAASAVGGTERSR
                     EFFSLLSDLLFLPNSPTLMNAGTPGGQLSACFVLPVEDSIEGIFTTLAHMALIHQSGG
                     GTGFSFSRLRPRGDIVNGVRGAATGPVSFMRVFDAATAAVRQGGRRRGANMGVLAASH
                     PDIEEFVTCKREGGLTNFNISVGVDEQFLRSLEGGLDYDLVNPRDGSVWKSINARSLW
                     RIIAESAWISGEPGVIFLDEINRRNTTPHLGLIEATNPCGEQPLYPYESCNLGSINLA
                     RCIRKSEIDDDLLVRVVRCGVEFLDSVIDVNHFPLPQIREKTLATRKIGLGVMGFAEA
                     LIRLDIPYESEEALRFAENLMEKVQTTARERSEELGVEKGSFPAIEGSVFHGDMRNAT
                     VTTIAPTGSIHLIAGTTSGIEPVFSFAYERTIDGQPVRIVSDLITEFLPPTAGGKDVV
                     EHVRRHGTVAGLPLDDHTLDLFKTALEITPEHHVRMQAAFQKYVDNAVSKTVNLPERA
                     TVGEISRIYSLAHELRCKGITVYRYRSRPDQVLSLGCDVCQIDG"
     gene            3374..3679
                     /gene="yciH"
                     /locus_tag="PK196_06500"
     CDS             3374..3679
                     /gene="yciH"
                     /locus_tag="PK196_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HON41102.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRITVKINKRRYGKEVTVI
                     EGLDPYDIDLDDLSKFLKAKLACGGTVKDSSIELQGNHRERVKALLAQKGYNLENIS"
     gene            3823..4152
                     /locus_tag="PK196_06505"
     CDS             3823..4152
                     /locus_tag="PK196_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HON41103.1"
                     /translation="MRCSFALDPDLMEQIDQFAREHSLDRNEAILELIEAGLSCKDAG
                     EAPVRQPRSFEEFNRLQQELEEVKNALAELKKEVRLVHHTIETDWSRETKGVPFQTKH
                     PWEFWRK"
     gene            complement(4157..4420)
                     /locus_tag="PK196_06510"
     CDS             complement(4157..4420)
                     /locus_tag="PK196_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41104.1"
                     /translation="MNEHERRLPVIGGRVACTQNEAVPVERCGLCVHSSRVVIAGREL
                     PSPARAYCSLCRGAPDIDMAKVEAVLCDDQRGEGFRSIANVIS"
     gene            complement(4413..4910)
                     /locus_tag="PK196_06515"
     CDS             complement(4413..4910)
                     /locus_tag="PK196_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HON41105.1"
                     /translation="MTEFECRLCGKCCMNAGDALIEIDRKLNSREYLCRQKIVGGTFR
                     ARVEERFLDAFRDTSEKDANPSWCPFLRPMPGRKGKYICTIHNTRPPICRSYICCSMR
                     IYDSSGEEAGKVKGRRSLVTEDPALRQCWEGEVAPLAIDDDNAWKKEVAAILGRAGYR
                     VEAYE"
     gene            5078..6241
                     /locus_tag="PK196_06520"
     CDS             5078..6241
                     /locus_tag="PK196_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HON41106.1"
                     /translation="MCGGEGTRLRPLTFGRPKPCIPIVNKPSIQHLVSHLSNLGFNDV
                     VVTLGYMSEAIEGALGNGSLFGVNITYVHEKTKLGTAGSVKNAQKYLDGQPFLVVGGD
                     HVVDLNLLELYREHLANDSITTIGLISIDDPTGYGIAEIDADCRIKRFREKPGPGEIF
                     SNLASTGIYACDPAIFDHIPAGEKFDFARNLFPDLMEEGYVLKGWPVRGNWSDVGNPR
                     SLREAERWKLQGISFTSISGDLAIRGGRILGPVHIGGFVSIGTNSRVIGPVSIGAGTA
                     IEENVIIGPYTSIGDGCIIKESAKIFSSSLYNRVVIGRGTTVSGSIIDNDTVIGNKCS
                     IEHDTVIGPQVVLKNDVVVHSGTRLWPEVIIPEGTVVKEHVLNEKYDARIEGS"
     gene            complement(6238..7026)
                     /locus_tag="PK196_06525"
     CDS             complement(6238..7026)
                     /locus_tag="PK196_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HON41107.1"
                     /translation="MDKKKVKDYMTYDVVTVDAHGTVKDVIDTIKKTHHDGFPVVENS
                     NRVVGYVSARDILFAHPSTPVEQVMSRHLIVADPEMSVNDAARVIFRSGIQKLPVLNE
                     KNELIGIISNADVIRSQIEHVSPEKVFKFMETLKKLYGVEPTLRRGSVPIDELLPTQS
                     KIYEDELEGRIYEIKKGLAEPLIVVRRPGRWILVDGHHRAIAAKRLGIKNLDAYIIEI
                     RDNIELGMERTARALNLNSLDDIKVLDYARHPLVALTHRQVGHG"
BASE COUNT         1494 a         2019 c         2081 g         1508 t
ORIGIN      
        1 ttttgagagc cggatctgtg caggcactgt ccggatagcg taaacaaatt gctgtaaatt
       61 ccataagccc tgatctctaa gggataaacg gagaacaggg atatggaaat aggaggtaag
      121 aggtcaggta tacctgacct cttacccatg cctcagcctg agaaacattt caggatcgcg
      181 tccatatcaa tatgcgcctc aaaatgcctg gccaggtcat cgtatgctgc ggatgcgccc
      241 agtatgccgc cgtccgcctc cgttataggc tcaaaaggca gcccccggtg tttcgagata
      301 taggcgagga gggcgtttac agctccgggg ttctggaaga gtccatgcat gtatgtcccg
      361 aagaccaggc cgtcaggcgt cgaagcgcct tcgccggcga acgcctctgg cagtcctcgc
      421 cgctctgtct cgcccatatg tatctcgtag ccctccacct cccccatagc ctggaggatg
      481 gggccagggc ctgttgcccg ccgcctgacc tgcaccgtcg tcttccggta gccggtaaat
      541 gtcgtgacga cgtcgaggag cccgaaaccc gtatactcgg cagggtcgtc tccagactcg
      601 ataccgctat cgatgatagc cttcccgagt atctggtatc ccccgcagat cccgatgatc
      661 gggacacccc tctccctgga aagtgcgagt tccttctcca cgccgtgccc gcgaaggacg
      721 ttgagatcct ccacggtgtt cttcgtcccg ggaaggatga tgcagtcgta gccggcaagc
      781 gattctcctg gcgtgacata ctccaccaca gcgtgccgct caaggagctc gaagtcggtg
      841 aagtttgaga tccgtgggag ccggatgacg gcgatcctga ttgcttctcc tgccacccct
      901 cttctcccgt cgtagatcga gagagagtcc tcgcttggaa tacgtatatc ggtgtatggc
      961 accacaccga ggaccggcac acctgtgagt tcttcgagtc tggcaacacc tgattcaaac
     1021 agcgcgggat cgccccggaa cttgttgatg atgactccct ttacaagcga tcggatctct
     1081 tcggggagga gggcaagagt gccgtagacc tgggcaaaga ccccgccccg ctctatatct
     1141 gcgaccagga gaactggaag gcggagcgcc agggcgagcc ttatgtttgc aatgtcacgg
     1201 tcgtagaggt tcacctcagc cgcccctccg gcaccctcca ccacaacgtt tccgaaccgt
     1261 ctctgcaacc gttcgaaagc ggcaaccgcc tctgcaagaa gtttatctgt ctccatgtag
     1321 tagtcccgga tctgcacatc cttgtaaggc cggccgagca gaaccacctg cgagaccgcc
     1381 tccccctttg gtttgaggag gatcgggttc atatcggcct ctggttcgat cccggccgcg
     1441 aaggcctggg cagcctgagc aatcccgatc tcactcccgt cggcggtgac gtaggagttg
     1501 aggctcatgt tctgggattt gaaaggcgca accgggatcc cgcgccggta gagcgcacgg
     1561 cagagtgctg caacggtcac gcttttgccg acgtgggatg cggttccgag cacgataaga
     1621 gacatcgcca gcagaagagt tggacacaac ggcttattaa ggccggggtt ccatgaaggg
     1681 taatatggaa ctcactccag cagcccgtct cctcctggaa cgccgctacc tccttccagg
     1741 cgagagtcct gagggtcttt tctggcgtgc cgccagcgcc gttggtggaa cggaacgatc
     1801 gcgcgagttc ttctcactcc tctcggatct ccttttcctg ccgaactcac ctaccctgat
     1861 gaacgctggc actcccggag gacaactctc cgcgtgcttt gtcctccctg tggaggactc
     1921 gattgagggt atctttacaa ccctcgcaca catggcgctc atccaccagt ccggcggggg
     1981 gaccgggttc tcgttctcgc gcctcaggcc tcgcggggac atagtcaacg gtgtgagggg
     2041 tgccgcaacc gggccggtct cgttcatgcg ggtctttgat gcagcgacag cggcggtacg
     2101 ccagggcggg cggaggaggg gggcaaacat gggcgtgctc gctgcctctc accccgacat
     2161 cgaggagttt gttacctgca aacgtgaggg cgggcttaca aacttcaaca tctctgtcgg
     2221 ggttgatgag cagttcctcc ggagcctgga gggtggtctc gactacgacc ttgtaaaccc
     2281 gcgtgacggc agcgtctgga agagtatcaa cgctcgctcg ctctggcgca tcattgctga
     2341 atcggcctgg atctcaggtg aacctggtgt aatctttctc gacgagatca accggcggaa
     2401 caccacaccg caccttggtc tcatcgaggc taccaaccca tgcggtgagc agcccctcta
     2461 cccctatgag agctgcaacc tggggagcat taacctggcc aggtgcatca ggaagagcga
     2521 gatcgatgat gacctgctgg tcagggtggt ccggtgcggc gtcgagtttc tcgactcggt
     2581 catcgatgtt aaccatttcc ctctccctca gatccgcgag aagaccctgg ccacaagaaa
     2641 gatcgggctc ggggtgatgg ggtttgccga agcgctcata cgcctcgata tcccatacga
     2701 atcggaggaa gcgctccggt ttgccgagaa cctgatggag aaggttcaga ccacggcgcg
     2761 ggagcgctca gaggaactgg gcgtggagaa gggctcgttt ccggcgatcg agggttcggt
     2821 cttccacggg gacatgcgca acgccaccgt cacaaccatt gctccaacgg ggtcgatcca
     2881 tcttatagcc gggacgacga gcggtatcga accggtcttc tcttttgcct acgagcggac
     2941 gatcgacggc cagcctgtga ggattgtaag cgatctgatc actgagttcc tgccccccac
     3001 tgccggcgga aaggacgtgg tcgaacacgt ccgccgccat ggaacggttg ctggcctccc
     3061 tctggacgac catacccttg acctattcaa gacggccctt gagatcaccc ccgagcacca
     3121 cgtccggatg caggccgcat tccagaagta tgtggataat gcggtctcaa agacggttaa
     3181 cctcccggag cgcgcaaccg tgggggagat cagtcggatc tattcccttg ctcatgaact
     3241 ccgctgtaaa gggattacag tctatcgtta tcgcagcagg ccagaccagg tgctatcact
     3301 cggctgtgac gtgtgccaga tcgatggatg accatgccaa ataatatata acgagagctg
     3361 aaaggtacat gcaatgaacg gtgggatctg tccgacatgc ggactgccaa aagaactgtg
     3421 catctgtgag gaagttgcaa aggaacaaca gagaattact gtaaagatca ataaacgtcg
     3481 gtatggaaaa gaggtcacgg tcatcgaggg cctcgaccca tatgacatag atctcgatga
     3541 cctctcaaag ttcctgaagg caaaactggc ctgtggcggt acggtcaagg actcttcgat
     3601 cgaactgcag ggcaaccatc gcgaacgggt gaaggccttg ctcgcgcaga aaggttataa
     3661 cctggaaaat atcagttgac ctgccccctt tcatggagag gcaggggaga attcattttt
     3721 tgcgagttgc tatatggtga gcggatcgtc tctcagagtt ctcggcagac agtataatca
     3781 gcaaagaggt tgtataatcg ggggtgttga cgacccatgg tgatgagatg ttcgtttgca
     3841 ctcgatcccg acctgatgga gcagatcgac cagtttgcga gagagcattc gctcgacaga
     3901 aatgaggcca tcctggagtt gatcgaggcc ggtctctcct gcaaggatgc tggagaggct
     3961 ccggtgcggc aaccgcgctc gtttgaagag tttaaccggc tgcagcagga acttgaggag
     4021 gtcaagaatg cccttgccga actcaagaag gaggtccggc tggttcatca cacgatcgag
     4081 accgactgga gccgcgagac aaaaggggtt ccgttccaga cgaaacaccc ctgggagttc
     4141 tggcggaagt gacctgctaa cttatgacgt ttgctatgct gcggaagccc tcgcccctct
     4201 ggtcgtcgca gaggacggcc tcaacctttg ccatatcgat atctggcgcg ccacggcaga
     4261 ggctgcagta agcccgcgcc ggtgacggca gttctcttcc tgcgatcacg acccgcgagg
     4321 aatggacaca gagcccgcat ctctccacgg gaaccgcctc gttctgcgta cacgcgaccc
     4381 ggcccccgat gaccgggagg cgacgttcat gctcattcat aggcctctac ccgataccct
     4441 gcacggccga ggatggcggc aacctccttc ttccaggcgt tatcgtcgtc gatcgcaagc
     4501 ggtgccacct ccccctccca gcactgccgc agtgcaggat cctccgtgac aaggcttcgc
     4561 ctgcccttca cctttcctgc ctcctctccg gaggagtcgt agattcgcat gctgcagcag
     4621 atgtatgacc tgcagatcgg tggacgggtg ttgtggatgg tgcagatgta cttccctttg
     4681 cggcccggca tgggccgcag gaaggggcac caggatgggt ttgcatcttt ctctgaggtg
     4741 tccctgaaag catcgaggaa ccgctcctcg acgcgggcgc gaaacgtccc gccgacgatc
     4801 ttctgccgac agagatactc acggctgttc agttttctat cgatctcgat gagagcgtct
     4861 ccggcgttca tacagcactt gccgcagagc ctgcactcaa actctgtcat tcaaggtgag
     4921 tctcttgcgc aggggaggat aaagattcgg aaagacccgc ccctggggtg cggggcggtt
     4981 ttcagcatgc ggcctgtatc aatgtgaccc gtgctgattc aatctcaata gggttaaatc
     5041 gatacgcaca caatggagtg gcatgaaggt gtgcataatg tgtggagggg aggggacacg
     5101 gctccgcccc ctcacattcg ggcgtccaaa accatgcatt ccaatcgtca acaaaccctc
     5161 gatccagcac ctggtctcac acctctcaaa cctggggttc aacgatgtgg tggtcaccct
     5221 cggttacatg agcgaggcca tcgagggggc gcttggcaac gggtcgctct ttggtgtcaa
     5281 tataacctac gtccacgaga agacaaaact agggacggct ggaagcgtca agaatgccca
     5341 gaaatacctt gatggccagc cgtttctggt cgttggcggc gatcacgtgg tggacttgaa
     5401 cctgcttgaa ctataccgcg aacaccttgc caacgactcg atcaccacca tcgggctgat
     5461 cagcatcgac gatcccacgg gttatgggat agccgagatc gatgcagact gccggataaa
     5521 acggttcagg gagaagcccg gcccagggga gatcttctcg aacctggcaa gcaccgggat
     5581 atatgcctgc gacccggcga tattcgatca catcccggca ggggagaagt ttgactttgc
     5641 ccggaacctt tttccagact tgatggaaga gggttatgtc cttaagggct ggcccgtccg
     5701 gggcaactgg tctgacgtgg ggaacccgag gtcgctgcgc gaggccgagc gctggaaact
     5761 gcaggggatc agtttcacca gcatctccgg agatcttgcc atcaggggcg gccgaatcct
     5821 tggtccggtg catattggcg ggtttgtctc gataggtaca aactccaggg tgatcgggcc
     5881 ggtctcgatc ggtgccggga ctgcaatcga ggagaacgtc atcatcggcc cttataccag
     5941 tatcggggat ggttgcatca tcaaggagag cgcgaagatc ttctcctcgt ccctctacaa
     6001 ccgggtggtg atcgggagag gcaccacggt gagcgggagc atcatcgaca acgatacagt
     6061 aatcggcaat aaatgcagca tcgagcacga cacggtgatc gggccgcagg tggtgctgaa
     6121 gaacgacgtg gttgtccatt ccgggacccg gctctggccg gaggtgatca tcccggaggg
     6181 gacggtggtc aaagagcacg tcttgaacga gaagtatgat gcccggatcg aggggtccta
     6241 gccgtgcccc acctgccggt gggtcagcgc gacaagcgga tggcgggcgt aatcgagcac
     6301 cttgatgtcg tcgagcgagt tgaggttgag cgccctggcc gtcctctcca tcccgagttc
     6361 aatgttatcc cggatctcga tgatgtaggc gtcaaggttc ttgatgccga gccttttcgc
     6421 tgctattgcc cggtgatggc cgtcgacaag gatccagcgg cccggccgcc ggacgacgat
     6481 cagcggttcg gcaagcccct tcttgatctc gtagatcctc ccttcgagtt catcctcgta
     6541 gatctttgac tgcgtcggca ggagttcgtc gatcgggacc gatccgcgcc tcaatgttgg
     6601 ttcgaccccg tagagtttct ttagagtctc catgaacttg aagaccttct ccggagatac
     6661 gtgctcgatc tgggatcgga taacgtctgc gttcgatatg atcccgatga gttcgttctt
     6721 ttcgttgagg accggcagtt tctgaatgcc ggagcgaaag atgacccggg cagcatcatt
     6781 cacgctcatc tccgggtcgg cgactatgag gtggcgggac ataacctgct cgaccggcgt
     6841 tgaggggtgg gcaaagagga tgtcccgcgc tgagacgtag cctacaaccc tgtttgagtt
     6901 ctccaccacc gggaaaccat cgtgatgcgt cttttttatt gtatcaatga cgtctttgac
     6961 cgttccgtga gcgtcgacgg tgacgacatc gtaggtcatg taatccttga ccttcttttt
     7021 gtccatcatg atagtctcac gctgggcggt catgaaccat tcggttatac ctggatgatg
     7081 ctcagaatcc atcagatcaa ca
//
LOCUS       DAOSZX010000076         6601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_9886_length_6601_cov_19.907424, whole genome shotgun sequence.
ACCESSION   DAOSZX010000076 DAOSZX010000000
VERSION     DAOSZX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_6601_cov_19.907424"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK196_06530"
     CDS             complement(<1..522)
                     /locus_tag="PK196_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HON41108.1"
                     /translation="MIGSAIGIDPELFSLVAVPLFIFCARICDVTIGTMRIIFVSRGV
                     KTVAPVLGFIEIFIWIVAISQIFQNLTNPINYLAYAGGFATGNYIGMHIEERLAMGLA
                     LVRIITQRDATNLIDYLRAAGYGVTVVDAEGRVGPSKVIFTVVRRRNLRDVENAIHQF
                     NPRAFYSIEDVRRA"
     gene            complement(604..1245)
                     /locus_tag="PK196_06535"
     CDS             complement(604..1245)
                     /locus_tag="PK196_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON41109.1"
                     /translation="MANEVVVLQPGDERAQKIARAMASQTANAIIQEFRSGPMTSSEV
                     ARKMKIPITTAAYHIDNLLEAGLLEVVNTRWSEKGREVKVYGLTDQVLIIAPPVSDLR
                     SVLKKYAMLFALIVLATLGLRVFLPAVLPPSWGAEPLLQRAGTGEKVSTFGVPGVDTA
                     GAPPVHGLVMGFFFGACVVLLALLVYEVFYWWRTSRRYRERLESEHDQSDETR"
     gene            complement(1235..3982)
                     /gene="alaS"
                     /locus_tag="PK196_06540"
     CDS             complement(1235..3982)
                     /gene="alaS"
                     /locus_tag="PK196_06540"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HON41110.1"
                     /translation="MLDDEYTLDYFRSQGFVRKVCKACGSAFWTRDPEQEFCGDSPCV
                     TYNFIGNPVFKPHTTDEMREAFLSFFERHGHTRLERYPVAARWRDDIYLTIASIADFQ
                     PFVTSGVVPPPANPLTISQPCIRLNDLDSVGRSGRHLTLFEMMAHHAFNTPEKQIYWK
                     DETVALCDEFITSIGGDPARVTYKEHPWYGGGNAGASVEVLIGGLEVATLVFMNLGRQ
                     KTDKPPVEVGGELYYPMRLRIVDTGYGLERFVWASRGSPTIYDAVFPDMVNRLMHSAH
                     LENLLDNPEFTKIMGLSARFAGVMDISGTNLHKLRRKVAEAIDVPVERLERIIVPIEK
                     VYAIADHTRCLAYMLGDCIVPSNVREGYLARLVLRRTLRMMNDLSMDEELADLVEAQM
                     EVVGIDNFEQDADVIREIISNEVARYASTLERGTRIVQKIAKSYRAKSSRIPLQEVIT
                     LYDSHGIPPEVVKEVAAAEGAVVEIPDNFYSLIAQMHSEAQKEASEDDHLAACRERAA
                     GLPPTKKLYYEFQNKVEFEAVVLDYFDGMAVLDQTLFYPEGGGQPSDTGTLVTPDNVV
                     RVEEVTKVGEVILHRVRGGPLKRGERVKGLVDEERRFSLMRHHTATHVLLYAAKKVLG
                     AHVHQAGAQKGGEVSRLDIRHYRHVTPEELRKIEIEANRMVMSNLPVQISVEERTKAE
                     QKYGFGLYQGGVPPGREIRIVRVGNDVQACAGTHVRSTGEIGLIRVMGVEHVQDGVER
                     LVFSAGLAAVRAVQQMEELLRASADVLSVQPENLPSTAARFFSEWKEQRKEIERLRSR
                     VVELERQNLEGEAVDGVRVVVRRVDAPQKELVALATSVADEGGVALIASVDGGARLVA
                     ASGTPAVNAADLVRDVCSLLGGKGGGKPNLAQGAGPDASRLEDALEQGRKMIIKALHG
                     E"
     gene            complement(4046..4363)
                     /locus_tag="PK196_06545"
     CDS             complement(4046..4363)
                     /locus_tag="PK196_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HON41111.1"
                     /translation="MNDVWQEIERKGALTPELRTSIERAYGERGRKALMAVDEGLVKR
                     YRDFFVVVGRSSEYVVESDFCTCSDFLFRGRECWHILAVRIAERTGLYESYDIWYQDT
                     WKP"
     gene            complement(4360..5070)
                     /locus_tag="PK196_06550"
     CDS             complement(4360..5070)
                     /locus_tag="PK196_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HON41112.1"
                     /translation="MDIKDFIAAMEEAAPPDLAEEYDEGRIGLIVEGRSEIEEVCCAL
                     DATERVVNAAVHAGADMLVVHHTPLWRPVTAIRGATSRLLSVILSLRMNLYVIHTNFD
                     RAEGGVNDVLASRLGLSRTERMTTGIVGDCSLDPGEIARLLSGGGIRVYGRVGVIRRL
                     AVVGGAGFDPDLIQEAANLGAEAFLSAELKHSVARASPIPCLEATHYALEAPAMEALA
                     SRMGWHYIADPPDVAIIP"
     gene            5277..6566
                     /locus_tag="PK196_06555"
     CDS             5277..6566
                     /locus_tag="PK196_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HON41113.1"
                     /translation="MRSFIIGMALLLLCAAIPAVSAQIGGDEGWYRVHCNIDGADVYF
                     DGRYQGTTYGGSLDLPVYTTGSPYSTIRVEKSGYHTYTGSLPSSPAAGETVDVYVTLQ
                     PVETAGSIHVTSSPSSAAIYLNGQYKGVTPLTIPDLSPGTYSLEAEHQGYQSDSSTVT
                     VRSGQQSSIQFTLTPIEQYGSIKVTSSPSGAYVYMDGKYRGQTPLTLTSVSAGKHTIE
                     LDLAGYYDWTSALSVSPGVTSSISAHLSPIPSETTGAIDVVSYPAGADVFLDEKYQGR
                     TPSAGVYTISNVQVGGHTVRVALSGYQDYTTSVIVSAATTSHVTATLQPGQPSTTGSI
                     SVTSSPAGAQVYIDNVYRGITPLTLDGIPTGTHQVRLALAGYSDYSTTVEVGAGSTSS
                     ASASLSPIQTPGTGMAPFAAVGALGLIGLLIAMRRRD"
BASE COUNT         1295 a         2110 c         1863 g         1333 t
ORIGIN      
        1 cgcccgccgg acgtcctcga tcgagtagaa cgccctgggg ttgaactgat ggatggcgtt
       61 ctccacgtcg cggaggtttc tcctcctgac caccgtgaat atgaccttgc ttggaccaac
      121 tcttccctcg gcgtctacga ccgtaacccc gtaaccggcg gcacggaggt agtcgatgag
      181 gttggttgca tcccgctggg tgatgatccg gacaagcgca agccccatcg ccagtcgttc
      241 ttcaatatgc atcccgatgt agttcccggt agcaaacccc cctgcgtagg caaggtagtt
      301 gatcgggttc gtgagattct gaaagatctg gctgatggcg acgatccaga tgaagatctc
      361 gataaacccc agaaccggcg caaccgtctt cacccctcgt gagacgaata tgatccgcat
      421 cgtccctatt gtgacatcac agatgcgtgc gcaaaaaatg aaaagaggga ctgcaaccag
      481 ggaaaaaagt tccgggtcga tcccgatggc gctaccgatc atgtatcatg atttgataag
      541 gtggtgatat agtcgtatcc atatttccag caatctgcaa agatgcggcg gccggtcacg
      601 ccctcaccgg gtttcgtctg actggtcgtg ctcactctcc agccgctcac ggtagcgccg
      661 ggatgttcgc caccagtaga acacctcgta gaccagcagc gcgagcagaa ccacgcaggc
      721 gccgaagaag aatcccatca caaggccgtg gacaggcggc gcccccgcgg tatcgacgcc
      781 cggcaccccg aacgtcgata ccttctcgcc cgtcccggcc ctctggagga ggggttctgc
      841 gccccaggac ggcggcaaca ccgccgggag gaagacccgg agccccagtg tagcaaggac
      901 gatcagcgca aacagcatcg cgtacttctt taagaccgac cggaggtcgc tcaccggcgg
      961 agcaatgatg agcacctggt ccgtaagccc gtagaccttc acctcgcgcc ccttctcgct
     1021 ccatcgggtg ttgacgacct cgaggagccc cgcttcaaga aggttatcga tgtggtatgc
     1081 ggcggtggtg atcgggatct tcatcttcct ggcgacctct gacgaggtca ttggccccga
     1141 cctgaactcc tgtatgatcg cgttagccgt ctggcttgcc attgcccggg cgatcttctg
     1201 cgccctctca tcgcccggct gaagaactac aacttcattc gccatgtaat gcttttataa
     1261 tcattttgcg gccttgctca agtgcatctt cgagcctgga ggcgtcaggc cccgccccct
     1321 gtgcaaggtt tggtttgccg ccgcctttcc cgccgaggag gctgcaaaca tcccggacaa
     1381 gatcggcggc gttcaccgcc ggcgttccgg aggccgccac cagccttgca ccaccgtcga
     1441 ccgacgcgat aagcgccacc ccgccctcat cggccaccga agttgcaagc gcgacgagtt
     1501 ccttttgggg tgcatcaacc ctccggacaa cgacccggac gccgtccacc gcctcgccct
     1561 caaggttctg cctctcaagt tcaaccactc tgctgcggag acgctcgatc tccttcctct
     1621 gctccttcca ctccgagaag aatcttgccg ctgtcgatgg caggttctcg ggctggacgc
     1681 tcaggacgtc tgcagacgcc cggaggagtt cttccatctg ctgcacagcg cgcacggcgg
     1741 cgagtccggc cgagaatacc agccgctcga caccgtcctg gacatgctcg acacccatga
     1801 cacggatcag cccgatctca ccggtcgagc ggacatgtgt tcccgcacac gcctgcacgt
     1861 cattcccgac ccggactatc cggatctccc ggccaggcgg gacacccccc tgatagaggc
     1921 caaacccgta cttctgctcg gcctttgtcc gctcctccac gcttatctgg acaggcaggt
     1981 tgctcatgac catacggttt gcctcgatct caatcttccg gagttcctcc ggcgtgacat
     2041 gcctgtagtg ccggatgtcc aggcgcgaga cctcaccacc cttctgggca ccggcctggt
     2101 ggacgtgggc gccaagcacc ttctttgcgg catacagaag gacgtgcgtt gcggtgtggt
     2161 ggcgcatcag cgagaaccgg cgctcctcgt ccaccaggcc cttcacccgt tcgccgcgct
     2221 tgagcgggcc gcccctgacc cgatggagga tcacctcgcc gacctttgtc acctcctcca
     2281 cccgcaccac gttatcaggc gtcaccagtg tcccggtatc ggatggctga cctcctcctt
     2341 cggggtagaa gagagtctgg tcgagcaccg ccatcccatc gaagtaatca agcaccaccg
     2401 cctcgaactc gaccttgttc tggaactcat aatacagttt ctttgtgggg ggcagacccg
     2461 ctgcccgctc gcggcaggcc gcgaggtggt catcttcgct ggcctctttc tgcgcttcgg
     2521 agtgcatctg ggcgatcagc gagtagaaat tgtcagggat ctcgaccaca gcgccctcgg
     2581 cggcagcaac ctccttgaca acctcgggcg ggatgccgtg cgagtcatac agcgtgatga
     2641 cctcctggag aggtatgcgt gaactctttg ccctgtaact ctttgcgatc ttctggacta
     2701 tcctggtccc gcgttcaagg gtgcttgcgt agcgtgccac ctcgtttgat atgatctccc
     2761 tgatcacgtc cgcgtcctgt tcaaagttat ctatacccac gacctccatc tgggcctcca
     2821 ccaggtcggc gagttcctcg tccatcgaga ggtcgttcat catccggagc gttcggcgaa
     2881 gcaccagccg cgcaaggtaa ccctcacgaa cgttcgaggg gacgatgcag tcgccaagca
     2941 tgtaggcgag acagcgggtg tggtcggcga tggcatagac cttctcgatc gggacgatta
     3001 tccgctccag ccgctccacc gggacatcga tggcctcggc gaccttccgg cggagtttgt
     3061 ggaggtttgt ccccgagata tccatcaccc cggcaaacct ggcgctgagc cccatgatct
     3121 ttgtgaactc cgggttgtcg aggaggttct cgaggtgcgc cgagtgcatc agtcggttca
     3181 ccatatccgg gaagaccgca tcgtagatcg ttggggaacc cctggacgcc catacaaatc
     3241 gctcaagacc gtagcctgta tccacgatcc tgagccgcat cgggtagtaa agttccccac
     3301 ccacctcgac gggcggtttg tcggtcttct gccgtccgag gttcatgaag accagcgtcg
     3361 cgacctcaag cccgccgatg agcacctcaa cagacgcccc ggcgtttccg ccgccgtacc
     3421 aggggtgctc tttgtatgtg acgcgggccg ggtcgccgcc gattgaggtg atgaactcgt
     3481 cgcagagggc gacggtctcg tccttccagt agatctgctt ctccggggtg ttgaaggcgt
     3541 ggtgtgccat catctcaaac agcgtcaggt gacgcccgga ccttccgaca gagtcgaggt
     3601 cgtttaaacg gatgcagggc tgggagattg tcagcgggtt tgccggcggg ggcacgaccc
     3661 cgcttgtgac aaacggctgg aaatcggcta tggaggcgat ggtcaggtag atgtcgtccc
     3721 tccagcgtgc ggcgacgggg tatcgttcga ggcgtgtgtg gccatgccgc tcgaagaacg
     3781 agaggaatgc ttcccgcatc tcatcggtgg tatggggttt gaagaccggg ttgccgatga
     3841 agttgtaagt cacgcagggg gagtctccac agaattcctg ttccggatcg cgtgtccaga
     3901 aagccgatcc gcaggccttg cagaccttcc gcacaaaccc ctgtgacctg aaatagtcga
     3961 gcgtatattc gtcatcgagc attgaaaatc acgcagcgta gataggtagg gttgtgaccg
     4021 atatagttgt tcgcacccgc cctgttcaag gtttccatgt atcctgatac cagatgtcgt
     4081 atgattcata cagtccagtt ctttcggcga tccgcaccgc aagtatgtgc cagcactccc
     4141 gtcctctgaa gaggaagtcg ctgcatgtgc agaaatcgct ctcaacaacg tactcgctgc
     4201 tgcggccgac cacaacgaag aagtcgcggt agcgttttac caggccctcg tcgacggcca
     4261 taagtgcttt tctcccccgt tccccatagg cacgctcgat ggaggtgcgc agttctggcg
     4321 tcagcgcccc cttccgctcg atctcctgcc agacatcatt catgggatta tcgctacatc
     4381 tggtggatcc gcaatataat gccagcccat ccgggatgca agcgcctcca tggccggggc
     4441 ctcgagggcg taatgggtgg cctcaaggca ggggatgggt gaagcacggg caacgctgtg
     4501 tttcaactcc gccgagagga aggcttcagc ccccagattt gcggcttcct ggatcaggtc
     4561 agggtcaaac cctgcaccac ccacgaccgc gaggcgccgg atcacgccca ccctgccgta
     4621 gacccggatc cccccgccgg agaggaggcg ggcgatctct cccgggtcaa gagagcagtc
     4681 gccgacgatg ccggtggtca tccgttctgt cctggaaagc ccgagccgtg acgccaggac
     4741 atcgtttaca ccgccttcgg cacggtcaaa gtttgtatgg atcacgtaga ggttcatcct
     4801 gagagagagg atgaccgaaa gcaggcgtga tgtcgccccc cggatagccg tgaccggtct
     4861 ccagagcggg gtgtggtgga cgaccagcat atccgcaccg gcgtgtactg cggcattcac
     4921 aaccctctcc gtggcgtcga gcgcacagca gacctcttcg atctcgcttc tcccctcgac
     4981 gatgagaccg atcctgcctt catcatactc ttcggcaagg tcgggtggtg cagcctcctc
     5041 cattgcggcg atgaagtcct tgatatccat attatatccc tacgatctca ggggataaaa
     5101 ggctgaacga gatgggggag acccaccttg aaaaatttgg ccgcctgcag gttattcccg
     5161 atcagagggg gtatctgccg tatatcgcgg aaaccacgga gtagaggccg gataaaatag
     5221 gaaaggttat ccgcagaaac gctgagagac gctcatcatc catcggagac ggtataatga
     5281 gatcttttat cattggaatg gcgctccttc ttctatgcgc cgccatccct gcggtatctg
     5341 cccagatcgg cggggatgaa ggatggtatc gcgtccactg caacatcgac ggtgccgacg
     5401 tctactttga cggccgctac cagggcacca catacggggg atcgcttgat ctcccggtct
     5461 acacaaccgg gtcgccctac agcacaatcc gggtcgagaa gagcggctac catacctaca
     5521 ccggcagcct cccatcgagt cctgcggcag gggagacggt ggacgtctac gtgacacttc
     5581 agcccgtcga gaccgccggt tcgatccacg tcacgtcgag cccttcaagc gctgcgatct
     5641 acttaaacgg gcagtataag ggtgtaaccc cgctcacgat ccccgatctg tctcccggca
     5701 cctactccct tgaggccgag caccagggtt accagtctga ttcctccact gttacggtca
     5761 ggtcggggca gcagtcgagc attcaattca ctctgacgcc gatcgagcag tatggctcga
     5821 tcaaggtcac atccagccca tctggcgcct atgtctatat ggatggaaaa tacaggggtc
     5881 aaacaccact gaccctcacg agcgtttctg ccgggaaaca caccatcgaa ctcgatctgg
     5941 ccggctacta tgactggacg tcggccctga gcgtgagtcc gggcgtgacc agttcaatca
     6001 gcgcccacct ctcgccaatc ccatccgaga ccaccggggc aattgacgtg gtctcctacc
     6061 ccgcaggggc ggatgtcttc ctggacgaga agtaccaggg cagaacaccg tcggcgggtg
     6121 tctacacgat atcgaacgtc caggtcggcg gccacaccgt gagggtggcc ctctccggat
     6181 accaggacta caccacgtcg gtcatcgtca gtgctgcaac cacaagccac gtcaccgcga
     6241 cgctccagcc aggtcaaccc tccacaacgg gctcgatctc ggtcacctcc tcgcccgcgg
     6301 gcgctcaggt ctacatcgat aacgtctacc ggggcatcac ccccctgaca ctcgatggta
     6361 ttcccaccgg aacccaccag gtcagactgg cgctcgcagg ctacagcgac tactcgacaa
     6421 ccgtggaggt cggtgcgggg agcacctctt cagcatcggc ctctctctca cccatccaga
     6481 cgccaggtac cgggatggcg ccgtttgctg cggtaggcgc gctcggcctc atcggtctcc
     6541 tgatcgccat gcgccggcgt gactgaggac caccccggca ccttttttcc acgaatcagc
     6601 g
//
LOCUS       DAOSZX010000077         6553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_9985_length_6553_cov_17.733303, whole genome shotgun sequence.
ACCESSION   DAOSZX010000077 DAOSZX010000000
VERSION     DAOSZX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9985_length_6553_cov_17.733303"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..226
                     /locus_tag="PK196_06560"
     CDS             59..226
                     /locus_tag="PK196_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41114.1"
                     /translation="MCKEEVEIARLVLVDTVTGTRIRHRIRNMKQALKQQEWYETALG
                     RRVRIEKEFRR"
     gene            706..1221
                     /locus_tag="PK196_06565"
     CDS             706..1221
                     /locus_tag="PK196_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41115.1"
                     /translation="MRGSPADRLICLILLLMLVPAAAWGATVTRTLSTAEPGAGEVFE
                     VSLSIEGITLGGVVETLPVGYSFVGTNHADDRILARDGQVAFALINETLITYQVRAPS
                     SGSGDITGIWEDFLGESNGTIPASHLAVDGIETEEENTPQERPTQEAAPPFALLTAIV
                     ALVIARRGGQR"
     gene            1218..2438
                     /locus_tag="PK196_06570"
     CDS             1218..2438
                     /locus_tag="PK196_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41116.1"
                     /translation="MNRRIILILVLPMLLAVQASAVEGGIPGDTDGDDVLTFSEYTAA
                     ALSYLDAAYMGGAGEIDRDDILDAAWVYARWDGKPVVVTDSSGRDLTLYRPLRRVVTF
                     SGEALETLRSVGFDMEKVVAVDKYTHEKSTFFPECQDKPDIGSIWSPDMERIISLRPD
                     AVFLYASISTSACDDIQRRLEASSPGIRVFRFDCFKPATYPEEIRKIAAIIDEPGQGE
                     EFASFYESTMDRIRKGTADIPADERVRVYFEYWTDYKTVAAGSGYNEKIEIAGGLNPF
                     AGESGEYPIIDPEAVIVMDPQVIVKLAGQGLAVGGYAHHDPETLAEIRSALLERPGWS
                     RISAVKDGRVYVIHSDILGGAQHFIGTAYLAKWFYPDRFADLDPHAIHQRYLSEFQGI
                     DFDLASMGSFVYPE"
     gene            2451..3503
                     /locus_tag="PK196_06575"
     CDS             2451..3503
                     /locus_tag="PK196_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON41117.1"
                     /translation="MEQSISLYEGYARLKRTRAAFMLALIFGLILLIGIAVTLGSADL
                     GVAESYLAILAGLFPGRFSAPELAITIVWDYRLHRVLFAVCAGFGLAVAGSVMQGILR
                     NPLASPFTLGIASAAATGASIAIVLGAGVLGGEYLIIGNAFLFALLAAFAIYGMARYR
                     GVGSETMVLAGIALMYLFSAVTSLLQYVGSSEELQAVVFWMFGSVDKSTWPKLGIVAL
                     VLACTVPYLVYRAWDLNALAEGDEVAAGLGVPVERSMTVMMLIASLITAVIICFTGTI
                     GFIGLVAPHITRMIIGSDFRTLLPASGLIGALLLLAADSIARSVLAPQVIPVGIVTAF
                     LGVPFFIYLFLHRRDA"
     gene            3503..4291
                     /locus_tag="PK196_06580"
     CDS             3503..4291
                     /locus_tag="PK196_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41118.1"
                     /translation="MPLISVEDLSFAYRDREVLHEITFPVERGEVLGLVGPNGCGKTT
                     LIRCINGMLSPRRGRVVFDDRDISNMHRREIARAMAYVPQSAGNRTAATVFETVLMGR
                     RPYLNWKVSPADEDKVVEALTLLDLEELALRKVAELSGGERQRVMIARALVQETDAIF
                     LDEPTSNLDLCHQMGVMEILRDLADKKGLAIIIALHDLNLAATYCHRLLVLEGGSIYG
                     CGAPAEVLTEDMLRSVYGIEAVVKRDLPAPYIVPLRPGCLESGV"
     gene            4403..4723
                     /locus_tag="PK196_06585"
     CDS             4403..4723
                     /locus_tag="PK196_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41119.1"
                     /translation="MDYIITGCVNPLFMGYTVRLDLPRILEVTGELQHFLELGTARLE
                     AEGPLSEEESEALIFSLADQLEDHLRTMRERQGSASIRDLQRWTRAWIEEQQEDLGGR
                     EDAG"
     gene            complement(4751..4915)
                     /locus_tag="PK196_06590"
     CDS             complement(4751..4915)
                     /locus_tag="PK196_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41120.1"
                     /translation="MAGRGKILCRLGLHRWGRKRGYDDYSSNVIEWEQRCERCGKKKR
                     WIEVKRDRRR"
     gene            complement(4975..6093)
                     /locus_tag="PK196_06595"
     CDS             complement(4975..6093)
                     /locus_tag="PK196_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HON41121.1"
                     /translation="MYYHLILTDACNLCCTYCRGKAFVVDSPDQPGITIDEDLPVDLD
                     IDLDDLYRFLAKDPDPVLILYGGEPLLAPDLVSEIARKAPVSALILHTNGTLLDGLEP
                     AIANRFDTILVSIDGPESLTDAHRGEGTFDRVMRNLRGLVAGGFGGELIARMTVAEDT
                     DIVEAVRYLTENERFSFPAVHWQMDANFWNDYGMRDYAAWVSESYNPGIRSLVAFWVE
                     TMRREARVLRWYPFIDPMEDMLLGRPGKLRCGCGHANYSIMTDGHIAPCPIMVGMKDY
                     YAGHIATADPLHLPVTAVGSPCTECGILEFCGGRCLYSNILRPWPEEGRRIVCSTVEN
                     LRAALSAALPHVRSLIRDGRIRMEDFSHRRYNSCEIIP"
     gene            6205..6540
                     /locus_tag="PK196_06600"
     CDS             6205..6540
                     /locus_tag="PK196_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON41122.1"
                     /translation="MSETETERLTERVIDPRGLVAYQPGSIVSRMLINTPAGTITIFA
                     FDADEGLSEHTAPYDAVLEVLEGEALISIAGTDYSLTAGEMIIMPAGKPHAVQAVTQF
                     KMMLTMIHA"
BASE COUNT         1273 a         1966 c         2001 g         1313 t
ORIGIN      
        1 cccggtctct cgcggcgatc tcgaatggaa acgttcgtaa acgtcgaggt ctatcttgat
       61 gtgtaaggag gaggtcgaga tagcgagact ggtgctggtc gacacggtga caggaacgcg
      121 gatcaggcac cgtatcagga atatgaagca ggccttgaag cagcaggagt ggtacgagac
      181 agccctcggg cgccgtgttc ggatcgagaa ggagttcagg cgataggtag gcaaagcggg
      241 ctcagagggg atatgatacc ttcagcagac gcattgaaga acctgctgca tgatacgccg
      301 ggatctctct cacgcaggga tgttgaagaa cctggcggtg gggttgcagg gctccctcct
      361 cttcaacggc ctctgaaaag gcctacagcg gccagtttgc aaaagggatc ctggtgctgc
      421 ttgggagcgg tttggcctct tcttcatgat tctatcaggt ctgctgacct gggcggtgac
      481 gataatctcg ctatcggcgg cagcgatgat gctcttcgtc gcaatggggt ttgaatggat
      541 gctgcaggcg ggggatgttg tatgtcaggc ggccccgggg tcatactacg tcacccgtca
      601 gccccaacaa gaccggagaa accctgatat acaccaatac actgaatttc tgatttgtta
      661 gcacaatagg aaacattcat attactcgat cgacaacaaa accttatgag aggctccccg
      721 gcagatcggc tcatctgcct tatcctgctg ctaatgctcg ttccggccgc agcatggggc
      781 gccacggtca cccggacgct ctccaccgcc gagccggggg cgggcgaggt ctttgaggtc
      841 tcactctcta tcgagggtat caccctcggc ggcgtcgtgg agactcttcc ggtcggttac
      901 tcctttgtgg gaaccaacca cgcggatgac cgcatcctgg ccagggacgg gcaggtcgcg
      961 tttgcgctga taaacgagac cctgataacc taccaggtca gggcgccctc ctccggcagc
     1021 ggggatataa ccggcatctg ggaggacttc ctgggcgagt caaacggcac catccccgcc
     1081 tcacatctcg cggtggacgg catcgagaca gaagaagaga acaccccgca ggagaggccg
     1141 acacaggagg ccgccccacc gtttgccctc cttacagcga tagttgctct cgtcatcgcc
     1201 cggcggggtg gtcagcgatg aaccggcgta tcatcctcat ccttgttctc cccatgctcc
     1261 tcgctgtcca ggcatcggcg gttgagggtg gcatcccggg ggacaccgat ggtgatgacg
     1321 tcctcacctt cagcgagtac accgcggcag ccctctccta tctcgacgcc gcctacatgg
     1381 gcggtgcggg agagatagac cgcgacgaca tcctggacgc cgcctgggtc tacgcccgct
     1441 gggatggaaa accggttgtg gtcaccgact catcggggag agacctgacg ctctaccgcc
     1501 ccctgcgccg ggttgtgacc ttcagcggtg aggctctcga gacgctgcgg tcggtcgggt
     1561 tcgatatgga gaaggttgtc gccgtcgaca agtacaccca cgaaaagagc acgttcttcc
     1621 ccgaatgcca ggataaaccg gacataggct cgatctggtc gccagatatg gagaggatca
     1681 tctccctccg gccggacgcc gtattcctct acgcgagtat cagcacatcg gcctgcgatg
     1741 atatccagcg caggcttgag gcgagcagtc ctggtatccg ggttttcagg ttcgactgct
     1801 ttaagcccgc aacctatccg gaggagatcc gaaagatcgc cgcgatcata gacgaacccg
     1861 ggcagggaga agagtttgcc tcattctacg agtcgacgat ggacaggatc cggaaaggca
     1921 cggcagatat accggccgat gagcgcgtcc gggtatactt cgagtactgg acggactaca
     1981 agaccgtcgc ggccggatcc gggtataacg agaagatcga gatcgccggt gggctgaacc
     2041 cctttgccgg cgagagcggg gagtatccca tcatcgaccc tgaggcggtc atcgtcatgg
     2101 accctcaggt gatcgtgaaa ctggccgggc agggcctggc cgtcggcggc tacgcccacc
     2161 acgaccctga aacgctggcc gagatcaggt cggcgctgct tgaacgcccc ggctggtcca
     2221 ggatctccgc cgtgaaggac ggtcgggtct acgtcatcca ttcggatatc cttggcggtg
     2281 cccagcactt catcgggacg gcttacctgg cgaagtggtt ctaccccgac aggttcgccg
     2341 acctcgaccc gcacgccatc caccagcgtt acttaagcga gttccaggga atcgatttcg
     2401 atctcgcgtc tatgggaagt ttcgtctacc ccgaatagga gccatcatcg atggagcaga
     2461 gtatcagtct ttacgagggc tacgcgcggc tgaagaggac acgcgccgcc tttatgcttg
     2521 ccctcatctt tggactcatc ctcctcatcg ggatcgcggt cacgctcggg tcggccgatc
     2581 tcggggtggc cgagtcctat ctcgcgatcc tggccggtct cttccccggc aggttcagtg
     2641 ctccggaact tgcaatcacc atcgtctggg actaccggct ccatcgggtg ctcttcgcgg
     2701 tctgcgccgg gttcgggctt gcggtggcgg gttcggtgat gcaggggatc ctccgaaacc
     2761 cgcttgcaag ccccttcacg ctcggtatcg cctcggccgc agccaccggc gcatccatcg
     2821 cgatagtcct tggcgccggg gttctcggcg gggagtacct catcataggc aacgccttcc
     2881 tctttgccct gcttgctgcg tttgccatct acgggatggc ccgatacagg ggagtgggct
     2941 cggagacgat ggtccttgcc gggatcgcgc tcatgtacct cttctcggcg gtgacctcgc
     3001 tcctccagta cgtcggcagc agcgaagagc tccaggcagt ggtattctgg atgttcgggt
     3061 cggttgataa gtcaacctgg ccgaaactcg ggatcgttgc gcttgtcctt gcctgcaccg
     3121 tcccttacct ggtataccgt gcctgggacc tcaacgccct ggcagagggt gacgaggtgg
     3181 cggccggtct cggggtgccg gtcgagcgtt cgatgacagt aatgatgctt atcgcctcgc
     3241 ttatcacagc ggtgatcatc tgtttcaccg ggacgatcgg gttcatcggg cttgttgcgc
     3301 cgcacatcac acgcatgatc ataggcagcg atttcaggac gctccttcct gcatccggtc
     3361 ttatcggtgc cctccttctc ctggcggcag acagtatcgc ccgaagcgtc cttgcaccgc
     3421 aggtcatccc cgtcggtatc gtgaccgcgt tcctcggggt gccgttcttc atctacctct
     3481 tcctgcaccg gagggatgcc tgatgccgct gatatcggtc gaagatctct cgtttgccta
     3541 ccgtgaccgg gaggttctcc acgaaatcac cttcccggtg gagagggggg aggttctcgg
     3601 gcttgttggg ccaaacggct gcgggaagac aacgctcatc cggtgcatca acgggatgct
     3661 ctctccaaga cgcgggaggg tggtgttcga tgatcgggat atcagcaaca tgcaccggcg
     3721 cgagattgcg cgggcgatgg cctacgtgcc gcagtccgcc gggaaccgga ccgctgcaac
     3781 tgtgtttgag accgtcctga tggggaggcg gccatacctg aactggaagg tgagccctgc
     3841 tgacgaggat aaggtggttg aggcgcttac cctcctcgat ctcgaagaac tcgcgctccg
     3901 gaaggttgcc gaactctccg gcggggagcg ccagcgggtt atgatcgcac gggcgcttgt
     3961 gcaggagaca gacgctatct tcctcgacga accgaccagc aacctggatc tctgccacca
     4021 gatgggggtg atggagatcc tgcgcgacct cgccgataag aaggggcttg ctatcatcat
     4081 cgcgctccac gacctcaatc tcgcggccac atactgccac cgtcttctgg tgctcgaagg
     4141 cgggtcgata tacggctgcg gggctccggc ggaagtgctc accgaagata tgctcaggtc
     4201 ggtctacggg attgaggcgg tggtaaaacg ggacctcccg gcgccttaca tcgtccccct
     4261 ccgccccggg tgcctggaaa gcggcgtata agcccacacg tccccaaaac gagggtgtgg
     4321 cgcggtatac cacccccggt ccgcgatacg accgggacga tatgaggggt acccgtggaa
     4381 gagtcagggg agccagaacg aaatggatta tattattaca gggtgcgtca accccctctt
     4441 catggggtat acagtcagac tggatctccc caggatcctg gaggtgacag gcgagttaca
     4501 acactttctc gagctcggga ccgcccgcct tgaggcggaa ggcccgttat cagaagagga
     4561 gtccgaagcg ctgatcttct cccttgcaga ccaacttgaa gaccacctga gaacgatgcg
     4621 cgagcggcag gggtctgcca gcatacgcga tctccagagg tggaccaggg cctggatcga
     4681 ggagcagcag gaggatctcg ggggaagaga ggacgcagga taaccgccgc tggccgatgc
     4741 gaatgattcc tcatctccgc cggtcccgct tcacctcgat ccagcgcttc ttcttcccgc
     4801 agcgttcgca ccgctgctcc cactcgatga cgtttgagga gtagtcatcg tagccgcgct
     4861 tccggcccca ccgatgaagc ccgagcctgc aaaggatctt tccgcgtccc gccatctttt
     4921 cactccagtt gttccccttt catccgaatg aatcttcgcc tggaggggtc ttcctcaggg
     4981 tatgatctca caactgttgt agcgccggtg ggaaaagtcc tccatcctga ttctgccgtc
     5041 ccggatgagc gatctgacat gcggcagcgc cgccgagaga gcggcacgga ggttctccac
     5101 cgtgctgcag acgatccgcc gcccctcctc cggccagggt cggaggatgt ttgagtaaag
     5161 gcagcggccg ccgcagaact caaggatacc acactccgtg caggggctcc caacagcggt
     5221 caccgggagg tggagaggat ccgccgtcgc tatgtgcccg gcgtagtagt ccttcatccc
     5281 gaccattata gggcatggag cgatatgccc atcggtcatg atgctgtagt tcgcgtggcc
     5341 gcaaccgcac cggagtttgc ccggccggcc gagcagcatg tcctccatcg ggtcaatgaa
     5401 ggggtaccac cggagcaccc gtgcctcccg ccgcatcgtc tcgacccaga aggccacaag
     5461 cgagcggatg ccggggttgt aactttctga tacccatgca gcgtagtctc gcatcccgta
     5521 gtcgttccag aaatttgcgt ccatctgcca gtggacggcc gggaaggaga acctctcgtt
     5581 ctccgtaaga tagcggaccg cctcaacgat atcagtatcc tcggccacgg tcatcctggc
     5641 gatcagttcg cctccaaacc cgccggccac gagaccgcgg aggtttctca tgacccgatc
     5701 aaacgtcccc tcgccccggt gggcgtcggt gaggctctcc gggccgtcga tcgagaccag
     5761 gatggtgtcg aaccggtttg cgatggcggg ttcgagacca tcaaggagcg tgccgttggt
     5821 gtgcaggatc agcgccgata caggcgcttt gcgggcgatc tcgctgacca gatcgggtgc
     5881 gagcaggggt tccccgccat ataagatgag taccggatcg gggtcttttg ccaggaaccg
     5941 gtagaggtca tcgagatcga tatcaaggtc aacaggcaga tcttcgtcga tggtgatccc
     6001 gggttggtcg ggggagtcga cgacaaacgc ctttccgcgg cagtaggtgc agcagaggtt
     6061 acaggcgtcg gtcaggatga gatggtagta cacgaaaggt ctcttattat tcagggtgta
     6121 tacgggaaaa aggtgaggtt tgatccgtcc tgctccccat tccatgccgc aaagcagaag
     6181 agcctgcaga ataaaacggc tcttatgtct gagactgaaa ccgagagact taccgaacgc
     6241 gtcatcgacc cccgcggcct ggtcgcctat cagccaggct cgatcgtcag ccggatgctc
     6301 ataaacaccc ctgcaggcac cataacgatc tttgccttcg atgcagacga aggcctctcg
     6361 gagcacaccg ctccctatga cgccgtcctg gaggttcttg agggagaggc tctcatcagc
     6421 atcgcgggca cggattactc cctcacagcg ggcgagatga tcataatgcc cgccgggaag
     6481 ccccacgctg tccaggccgt cacgcagttc aagatgatgc tgacgatgat ccatgcctga
     6541 cccactcccc cgg
//
LOCUS       DAOSZX010000078         6466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10143_length_6466_cov_20.457651, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000078 DAOSZX010000000
VERSION     DAOSZX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10143_length_6466_cov_20.457651"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..391
                     /locus_tag="PK196_06605"
     CDS             53..391
                     /locus_tag="PK196_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON41123.1"
                     /translation="MKIVDVSKVPVNENPHHVDARKVYDTEHATAVVITLAPGEALKK
                     HITPVDVFFYVLEGTGVVEIGDERATVGRDHLVESPAKIPHRWINESDRPFRVLVVKV
                     PRPASGTRVL"
     gene            402..1160
                     /locus_tag="PK196_06610"
     CDS             402..1160
                     /locus_tag="PK196_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41124.1"
                     /translation="MPSGSITMIAGVVYAYLAFAVLALLWYSRRFTRRRALVFLAASA
                     LLGFLVFAPVFPFMVQEVVLGNLPVLIGLVVFIVLVFLAGRIICGQVCAVGAVQELVY
                     LIPVKKHRRTANLRQAAIRAAVFIVILVAGAGFSVNLLALLGLRAFFNLRVASIPFVI
                     FLAILLVSVFFYRPFCRYICPYGALLAPVSSRAVYRLRRTDACINCRKCERACPTGAA
                     DPDARFGECYLCGRCTEACPVEGALVYARKGGKE"
     gene            1181..1516
                     /locus_tag="PK196_06615"
     CDS             1181..1516
                     /locus_tag="PK196_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON41125.1"
                     /translation="MPDPKRAELRGKVLRLADLVAYQEGTVASRMIINKPAGSITIFS
                     FDENEGLSEHTAPYDAVVTILDGECEVWVAGETHQMREGDTIIFPANVPHALSAITPF
                     KMSLTMIRE"
     gene            1524..1799
                     /locus_tag="PK196_06620"
     CDS             1524..1799
                     /locus_tag="PK196_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HON41126.1"
                     /translation="MTNEKEGDYCTICGGIRPDAIKIRTIPVDGKDTGINQLDFIMDG
                     VRKLNLKDDAAIRAELLRWAGEFNYIPTKKREAYGDALLAEYRKTKE"
     gene            complement(1818..2285)
                     /locus_tag="PK196_06625"
     CDS             complement(1818..2285)
                     /locus_tag="PK196_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HON41127.1"
                     /translation="MSLEIAVAAPFKHMRKERLQKSEFIFYVAIDRKWMNKEQANLLL
                     ERAMEEGLVEMEGGTIRPLFDLAAVEIPLGFKPTSDLLTGAATPCDELIARIASAAGK
                     PVREIAAEINQVIDSGFDGNLRLEAAAVILAKRYGVQFEDKLPALEKSVAKKG"
     gene            complement(2282..2704)
                     /locus_tag="PK196_06630"
     CDS             complement(2282..2704)
                     /locus_tag="PK196_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41128.1"
                     /translation="MRRIYYRFPQSIDLDFEIARPFRDVLACIARLNNTHATRRGPGG
                     LIKERVVIQIAEGRTRFGDLDDLSPLEETVAETADFRVDLQKTLLTPEKRLPISENVF
                     YLRIVDKGAVTECYVAKEGLHGDLDAIDLRTILKGACE"
     gene            complement(2917..3294)
                     /locus_tag="PK196_06635"
     CDS             complement(2917..3294)
                     /locus_tag="PK196_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HON41129.1"
                     /translation="MLWQGRSVRKPSGGRYHTCRGKKRSEIGRAPAETHIGEDRRRVV
                     RTYGGNRKVRALLLEYASVADPATGEIRKAKIETVEENPANPNYVRRNLLTRGAIIRT
                     EAGRARIVSRPSQDGVVNAVLLS"
     gene            complement(3404..4051)
                     /gene="hypB"
                     /locus_tag="PK196_06640"
     CDS             complement(3404..4051)
                     /gene="hypB"
                     /locus_tag="PK196_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HON41130.1"
                     /translation="MHQIDIHLEKDIYEANNRLADANAAHLQDHGVRAFDLLGAIGSG
                     KTSLIERLVPLIRERGLRAGAIAGDVYGDDDFQRIVALGIPAYNANTGKECHLDAHLV
                     EHALEQMPLDDIDILFIENVGNMVCPTDFRLGAEKRIVVVSSTEGDDVVNKHPMMFRG
                     GDIGVINKIDLAPFVGANLDRMESDIRRYNPGMKVFRTNLKTGDGLEGLLDTILE"
     gene            4108..4386
                     /locus_tag="PK196_06645"
     CDS             4108..4386
                     /locus_tag="PK196_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HON41131.1"
                     /translation="MSAERILYPCYFDASLRRREGRRVARNLAVKSPDTSAIATVLRK
                     MNLPHRLEGHHHPARWVEREGRVVVEWKGSKEDLIRKVAAGLSGRRGA"
     gene            4386..5030
                     /locus_tag="PK196_06650"
     CDS             4386..5030
                     /locus_tag="PK196_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HON41132.1"
                     /translation="MYDLHTHTILSDGELLPIELVRRAAVLGYKTIAITDHADTSNLA
                     CLVEAVSRCREPAECYGVNLLVGVEITHLPPSMIPAAAREAKRLGADIVVVHGESVLE
                     PVAPGTNRTACNCDDVDVLAHPGLVTLDDARAAAEHGVALEITARTGHNRTNGHVVRV
                     AQKAGCRLVVNSDAHNPSDLMSREVRWALTVGAGLTEEESRRILSLDVLEFLRK"
     gene            5369..6382
                     /locus_tag="PK196_06655"
     CDS             5369..6382
                     /locus_tag="PK196_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HON41133.1"
                     /translation="MAEVEKLKQLQLQREALKKREEQKVKETEKTRPEESVESVCPEC
                     GSHQLVHDYERAELVCQSCGLVLDEEFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHD
                     KGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMA
                     SALGLPRNVRETAAVVYRDAVEKNLIRGRSIEGVAAAALYAACRQCSVPRTLDEIAEV
                     SRVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGE
                     RELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIE
                     IIL"
     gene            6394..6465
                     /locus_tag="PK196_06660"
     tRNA            6394..6465
                     /locus_tag="PK196_06660"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6426..6428,aa:Ala,seq:cgc)
BASE COUNT         1314 a         1914 c         1953 g         1285 t
ORIGIN      
        1 gcaaactact ggacaaatta taaaggttga acaccatcag gagatgatga agatgaagat
       61 tgttgacgta tcaaaagtgc ctgtaaacga aaacccccac catgtcgacg ccaggaaggt
      121 ctacgacacc gagcacgcga cagcggtggt gataacgctt gcgcccggcg aggcgttaaa
      181 gaagcacata accccggtgg atgtcttctt ctacgtcctc gaagggacag gggtcgtcga
      241 gataggcgac gagcgggcaa ccgtcggccg cgaccacctt gtcgagagcc cggcaaagat
      301 cccgcaccgc tggatcaacg agagcgaccg tccattccgg gtgctcgtcg taaaggtccc
      361 tcggccggcg agcggaacca gggtcctgtg aggcagcagg tatgccttcc ggatcaatca
      421 cgatgatcgc gggggtggtc tacgcctacc ttgccttcgc cgtcctcgcc ctgctctggt
      481 actccaggcg gttcacaagg cggcgagcgc tggtcttcct ggcagcatcg gcgcttctcg
      541 ggttcctggt ctttgcaccg gtcttcccat tcatggtgca ggaggtcgtc ctcgggaacc
      601 tgccggtgct catagggctg gttgtattca tcgtccttgt cttcctcgcg ggccggataa
      661 tctgcggcca ggtctgcgca gtgggtgccg tccaggaact cgtctacctg atcccggtta
      721 agaaacaccg ccgcaccgca aacctgaggc aggcggcgat cagggccgcg gtctttatcg
      781 tcatcctggt cgccggagcg gggttctcgg tgaacctcct cgcgctcctg ggtctccggg
      841 ccttcttcaa cctccgggtc gcaagcattc ccttcgtcat cttccttgcc atcctgctcg
      901 tctcggtatt cttctaccgg ccgttctgcc gctacatctg cccctacggc gccctgctcg
      961 ccccggtctc gtcacgggcg gtctaccggc tgcggcggac ggatgcctgc atcaattgca
     1021 ggaagtgcga acgggcgtgc cccacagggg cggccgaccc ggacgccaga ttcggcgagt
     1081 gctacctctg cggcaggtgc accgaggcct gcccggtgga gggggcgctt gtctacgccc
     1141 ggaaaggcgg caaagagtag aatcacgggg tgaaaaaaga gtgccagatc ctaaacgcgc
     1201 agagttgcgg gggaaggtcc tccgcctggc tgaccttgtc gcataccagg aaggaaccgt
     1261 tgcaagcagg atgatcatca acaaaccggc ggggagcatc accatattct cgttcgacga
     1321 gaacgaaggg ctctcggagc acaccgcacc gtatgacgcg gtggtgacga tccttgacgg
     1381 cgagtgcgag gtctgggtcg cgggcgagac gcaccagatg cgggagggcg atacgatcat
     1441 cttcccggca aacgtccccc atgcgctctc ggccataacc ccgttcaaga tgtcgctcac
     1501 catgatcagg gagtgaacag gagatgacaa acgagaaaga gggtgattac tgcaccatct
     1561 gcggcgggat ccggcccgac gccataaaga tcaggacgat cccggtggac ggcaaggata
     1621 cggggataaa ccagctcgac ttcatcatgg atggcgtgcg aaaactcaac ctcaaggacg
     1681 acgccgcgat ccgcgccgaa ctcctccggt gggcgggcga gttcaactac ataccgacaa
     1741 agaagcgaga ggcatacggc gacgcgctgc tggcggagta caggaaaacg aaggagtgat
     1801 gcggagagac cggccggcta ccccttcttt gcaaccgact tctcaagcgc gggcagtttg
     1861 tcctcaaact gcaccccgta cctcttcgcg agtatgaccg ccgccgcctc aaggcggagg
     1921 ttgccgtcaa agccgctgtc gatgacctgg ttgatctctg cggcaatctc ccggaccggc
     1981 ttgccggccg cggaagcgat ccgggctatc agttcgtcgc agggggtcgc cgcccctgtc
     2041 aggagatcgg aagtgggctt gaacccgagc ggtatctcga ccgcagcgag atcgaagagc
     2101 ggccggatgg ttcccccttc catctcgacc agcccttctt ccatcgcccg ctcaaggagc
     2161 aggttcgcct gctccttgtt catccatttc cggtcgatgg caacgtagaa gataaactcg
     2221 ctcttctgca gccgctcctt gcgcatatgc ttgaacggcg cggcaaccgc aatctcaaga
     2281 ctcactcgca ggcacccttg agtatcgttc gcagatcgat tgcatcaagg tcgccgtgca
     2341 acccctcttt tgcaacgtag cactcggtca ccgcgccttt atcgacgatc cggaggtaga
     2401 agacgttctc tgagatcggc agccgttttt ccggcgttag cagggtcttc tggaggtcga
     2461 ccctgaagtc cgcggtctcg gcgaccgtct cctcaagcgg cgataggtca tcgaggtccc
     2521 caaaccgcgt ccgaccttca gcgatctgga tgacgacccg ctccttgatg agaccccccg
     2581 ggccgcgccg ggtggcatgg gtgttgttca gacgcgctat gcaggccagg acatcccgga
     2641 acggccgggc gatctcaaag tccaggtcaa ttgattgggg aaaccggtaa tatatcctgc
     2701 gcatcactag aactgacgtc cgcggaacgt aaaaaggatc tgatcccttg cagtgcaccc
     2761 ccggagggcg gcgggggtgt tccgcaaaag acgttcaatc agagatggag agaggaggaa
     2821 acctcctttc ccccgatacg cgtcacccca ttcccgggca cggccccaca caggcaggag
     2881 tgtcctgcag cctgccagag caagtgaaaa agtatcttat gagagcagga cagcgttgac
     2941 gacgccgtcc tggcttggcc tgctgacgat ccgcgcgcgg ccggcctctg tcctgatgat
     3001 tgcacccctg gtcaggaggt tccgccggac gtagttgggg ttggcggggt tctcctcgac
     3061 cgtctcgatc tttgcttttc tgatctcgcc ggttgccggg tctgccacac tcgcgtattc
     3121 cagcaggagc gcccggactt tccggttgcc accataagtc cggacaaccc ttctgcggtc
     3181 ttcacctatg tgcgtctctg ccggagctct cccgatctcc gacctcttct tgccccggca
     3241 ggtatggtaa cgcccgcccg atggctttct tactgatctt ccctgccaca acatgtgacc
     3301 accgaaatac tttctgttga ggacgcggtt ccccgggtca ggggcagcag tctctcacta
     3361 aagtgctata aatgtacgag agcaggatat ttaactctgc tgcttactcc aggatcgtgt
     3421 ccagcagccc ctccagcccg tcccccgtct tcaggttcgt ccggaagacc ttcatccccg
     3481 ggttgtagcg gcggatatcc gactccatcc ggtcaaggtt ggcgccgaca aacggtgcga
     3541 gatcgatctt gttgatgaca cctatatctc cgccccgaaa catcatagga tgtttgttca
     3601 ccacgtcatc gccctcggtc gaactgacaa caacgatcct cttctcagcg ccaagccgga
     3661 aatcggtcgg gcagaccatg ttgccgacat tctctatgaa gaggatgtcg atatcgtcaa
     3721 gcggcatctg ttcaagggcg tgttcgacca ggtgggcatc aagatggcac tctttcccgg
     3781 tgtttgcgtt gtaggccggg atcccgaggg cgacgatccg ctggaaatcg tcgtcaccat
     3841 agacatcacc ggcgatcgca cctgcacgga gaccgcgttc gcggatcagc ggcaccagcc
     3901 gttcgatcag ggaggtcttc cccgatccga tggcgccgag aaggtcaaac gcccggacgc
     3961 cgtggtcctg aaggtgagcg gcgttggcat ctgccagacg gttgttggcc tcgtatatat
     4021 ccttctccag gtggatgtca atctgatgca tggtacatta ctgtgatcat gatctgttta
     4081 taggttaacc gcgaagatca tagagcaatg agcgctgaac gcatcctgta tccctgttac
     4141 tttgacgcgt cactccggcg gcgcgagggg cgccgggtcg ccagaaacct cgcagtgaag
     4201 tcacccgaca cctctgccat cgcgaccgtt ctgcggaaga tgaacctgcc gcaccgactt
     4261 gaggggcacc accaccccgc tcgctgggtg gagcgtgaag gccgggtcgt tgtggaatgg
     4321 aaagggagca aagaggacct tatacggaag gttgcagccg gtctatccgg gcggaggggt
     4381 gcgtgatgta cgaccttcat acccatacca tcctctctga cggcgaactc ctccccatag
     4441 aactcgtccg ccgcgccgcc gtcctcggct ataagaccat cgccatcacc gaccacgcgg
     4501 acacctccaa cctggcatgc ctggtggagg ctgtgagcag gtgccgggaa ccggcggagt
     4561 gttacggcgt gaaccttctt gtgggggtgg agatcacgca cctcccgcca tccatgatcc
     4621 cggcggccgc acgagaagca aaacggcttg gcgccgatat cgtcgttgtt cacggcgagt
     4681 ctgtcctgga gccggtcgcc ccggggacga accgcaccgc ctgcaactgc gatgatgtcg
     4741 atgtgctcgc ccacccgggg ctcgtgacgc tcgacgatgc gcgcgcggcc gcggagcacg
     4801 gggttgcgct cgagataacc gcacgcacgg ggcacaaccg caccaacggc cacgtggtga
     4861 gggtggcgca gaaggcaggg tgccggcttg tggtaaattc tgatgcgcat aatccctccg
     4921 atctgatgtc aagggaggta cggtgggcgc ttacggttgg cgcggggttg acggaagagg
     4981 aatcccggcg gattctctcc ctggacgtac ttgagttcct tcgaaaataa gatattgtat
     5041 ttataattta gcaaaacttt tttatagatc tatgataaat atatatacgt tactaaatat
     5101 ttctagcaat attactgtcc agaggtagca ggttgcggtt aatcggtcac tctgtaggtg
     5161 tttgcagcaa tcgcttgtta atcttgcgat gtgatgctgc acaactgccc cgcctctacg
     5221 gtgaggttgt ggatcgccgg ttaaaacccc tgggaaaggt agtcgagata ttcgggaata
     5281 tatcatcgcc atatgctgta gtcctctgct acaatggctg ccctgtgcag actggcgaga
     5341 aagtctttgc gaaataggtg tataatcaat ggctgaagtg gaaaagttaa aacaactgca
     5401 gttgcagcgc gaggccctta aaaaaaggga ggagcagaaa gtcaaggaga ccgagaagac
     5461 gcgccccgaa gagagtgtgg agtccgtctg cccggagtgc ggcagccacc agcttgtcca
     5521 tgactacgag cgtgccgaac tggtatgtca gagttgtggt cttgtcctcg acgaggagtt
     5581 catcgaccgc ggtcctgagt ggcgggcgtt cgaccacgac cagcgcatga agcggtcacg
     5641 tgtcggtgcg ccgatgacct tcaccatcca cgacaagggt ctctcgacga tgatcgactg
     5701 gaggaaccgg gattcatatg gccgcgctat ctcaagcaag aaccgggcgc agctctaccg
     5761 cctcaggaag tggcagcggc gtatccgggt ctcaaacgcc acagagcgga acctggcgtt
     5821 cgcgctctcg gagctggacc ggatggcctc agctcttggt ctgccgcgga acgtgcgcga
     5881 gaccgccgcg gtcgtctacc gtgacgccgt ggagaagaac ctgatccgcg gccggagtat
     5941 agagggcgtt gcggcggcgg cgctgtatgc ggcgtgccgc cagtgcagcg ttcccaggac
     6001 actcgatgag atcgccgagg tctcccgtgt ctcgaggaag gagatagggc gtacctacag
     6061 gttcatctca cgcgagctcg gccttaaact cctgccgacg tcgccgatcg actacgtacc
     6121 gcgcttctgc tcggggctga acctgaaggg cgaggtccag agccgggcgg tcgagatcct
     6181 ccggcaggca ggcgaacgcg aacttacgag cggcaggggc ccgacgggtg ttgcagcggc
     6241 cgcaatttac atctcctcga tcctgggcgg cgagaggcgc acccagcgcg aggtggccga
     6301 ggtggccggc gtgaccgagg tcacgatcag gaacagatat aaggaactgg cagagaagtt
     6361 agatattgag atcatactct gatctcatat cacgggcccg tagatcaggg gtagatcgtt
     6421 gcgttcgcaa cgcaaaggcc gcgggttcaa atcccgccgg gtccat
//
LOCUS       DAOSZX010000079         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10190_length_6445_cov_22.317840, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000079 DAOSZX010000000
VERSION     DAOSZX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_6445_cov_22.317840"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK196_06665"
     CDS             complement(<1..182)
                     /locus_tag="PK196_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HON41134.1"
                     /translation="MAAKGDMLYAWTTSPDIAGVAECGGAVTGLLKYALENDIVDAVL
                     AVKKGVDIYDAVPTLIT"
     gene            complement(184..2253)
                     /gene="fdhF"
                     /locus_tag="PK196_06670"
     CDS             complement(184..2253)
                     /gene="fdhF"
                     /locus_tag="PK196_06670"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HON41135.1"
                     /translation="MTDTSEKLRYVHTTCPYCGVGCGLNLVVSDGKLVGVEPYKRTPI
                     NEGKLCPKGMTCWEFVQSPDRLTKPLIKKNGEFVEATWDEALDLVASKFKETYDNYGP
                     KSLGFQVSCRTPNEECYIMQKLARVAFKTNNVDNCARICHGPSVAGLSLSFGSGAATN
                     PFEDVLNADVIFMIGANTIEAHPLAGRRIVQAKKAGKTIIVCDPRYTPTARLADHYVR
                     YNPSTNIALINSIMYWIIQQNLHDREFIEKRTTGFEELKKTVEKYADVESITGVPTER
                     IKEIAQIYGSANNAVIIYCLGITELTTGTDNVRSLGNLAMLTGNVGRPGTGVNPLRGQ
                     NNVQGACDMGAYPNVYSGYQKCEDDATRKRMEELWGVTGLPGEYGATLIEQINQCGDP
                     IRAMYIFALNPVVSYPDSNHVMRSLEKLDFLVVQDIFMTETAKYADVILPGVSFAEKD
                     GTFTSGERRVNRVRKAVEPPGEARPDWDIFVDLAHRLGLKGFDFNSPEDIWNDVRRVT
                     PSMAGISYERIEKPESLHWPCPTVEHPGTPILHREKFSSADGLGHFFGIEYRPPAEVA
                     DEEYPFTLMTGRLLFHYHTRTQTGRAKILHHEVPEAYVQINTEDAARLNIKNGEKIRL
                     RSRRGEAEALARVTDEVGPGVLMMTMHFSGKGSVNQLTNTALDPMSKMPELKHSAVAV
                     EKITEVQ"
     gene            2545..3204
                     /locus_tag="PK196_06675"
     CDS             2545..3204
                     /locus_tag="PK196_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HON41136.1"
                     /translation="MIKHSYVQPRLNHTWEDIEARLREKGTSPELIENFRRCIEFHTY
                     PAPGLLVGVYMVDYALELLKPPEGKKIYAVCETTKCLPDALQVMAHCTTGNHRLRVIP
                     IGKFAITLNGPADAPYVNGVRVFVDGDKMQRYPTFALWYTKDPLFDPRTRGTALIDEI
                     IDAGRDLLSHEQVRVKVPQKLPWKSAVCSICGEIVPDNMLVNGVCSDCRSQSYYEKVT
                     C"
     gene            3261..4076
                     /gene="tsaA"
                     /locus_tag="PK196_06680"
     CDS             3261..4076
                     /gene="tsaA"
                     /locus_tag="PK196_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HON41137.1"
                     /translation="MELSPIGLVHSSIRSRSDMPIQGVSAEVEIFPEYVAGLEGVEEN
                     SHLILVCWLHQADRSVLKAVARKISDELPEKGVFSLRSPARPNPLSISVVRLFGVREG
                     RYLLLEHLDLIDGTPVIDIKPYQPGWDCVFSAAGHDRTEKIRRMKPGDYRASLIREAV
                     NYHGDVCCGVAIGMRIAEAATRILDCDLRHPGVVVAPGADPCILDALIGITGATPGNR
                     RLRCSEGRRYAVSSSEKEVVFRLLAAPQSVDDIFAAEETSLFECTVHNRPQPK"
     gene            4100..5938
                     /locus_tag="PK196_06685"
     CDS             4100..5938
                     /locus_tag="PK196_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HON41138.1"
                     /translation="MTHRYLNLTPLSRALAVMRQKFPPPGRAESVPLQLAVGRVTAEP
                     IYAVYSVPMVDTAKFDGYAVRSGETLGAQDQRPLALTNYSRINTGEVLPQAFDAVIMV
                     EDTWEENDRVWIRKSAARGQNIRRAGEDIRAGELVLPKGHQIRPFDIGAVATYGITSV
                     MVRAVRVGIIPTGSDLVPPGTLPRPGQTVETNTLMAEAYLSGMGATCRRYGVVPDEPH
                     RIREAVAEAVAENDLVLLSAGTSTGSRDFSREAVAELGEILFHGIAMRPGKPVLLAGL
                     DGKPVFGMPGYPVSAQTVLREVVANLLVWWGLAPPPVAEMDARLASRLASDLGFDEFV
                     PVSVGRVNGTCWATPHPRGGGIQMAVVRANGYLHVPAWREGIEAGEVVRVSLTAPSAS
                     IARTIVCVGKRDPALEKLGNRLQDSGYLLHCCNASTMGAVLALRANTCHAASIVLPDT
                     ESAWNDQVFRYLPGVNLLRLPLARTELGIASTGELDPERLALLRVANRPKSQAAQVLL
                     DAWLEREGIGYPHQDQDLAAGAKVRVCAGAEAREARLSFIPIGYESCNLVIRGEVASE
                     GGIAALIRTTRSQEFRAYLRTIGREPAGGAGDEPLPARDAAPARNQ"
     gene            5964..6368
                     /locus_tag="PK196_06690"
     CDS             5964..6368
                     /locus_tag="PK196_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HON41139.1"
                     /translation="MRVSEQTRDQVMAVLRRLLEAAGRKDAGGILALTDADFRGFWSV
                     PGEKVIGKEALRRHLERGFTQVDAVTLEFSSVHIGAEGTVAWVMADLTCNLVRGGAGQ
                     TMDGLVTAVLRGTGHAWIFAQIHCSFPAIRGR"
BASE COUNT         1254 a         1876 c         1957 g         1358 t
ORIGIN      
        1 gtgatcaggg tcgggacggc gtcgtagatg tccacacctt tcttcaccgc cagcacggca
       61 tcgacgatat cgttctccag ggcatacttg agcagcccgg tcaccgcacc gccgcactca
      121 gcgacgccgg caatatcggg gctcgtcgtc caggcgtata gcatatctcc cttggctgcc
      181 atctcactgc acctccgtta tcttctcgac ggcaaccgca ctgtgcttta actccggcat
      241 cttcgacatc ggatcgagcg cggtgttcgt cagctggttc acagacccct tcccgctgaa
      301 gtgcatcgtc atcatcagca ccccgggtcc gacctcgtcg gtcaccctgg caagggcctc
      361 cgcctctccg cgcctgctgc ggagccggat cttttctccg ttcttgatgt taagccgcgc
      421 tgcatcctct gtgttgatct gcacataggc ttcgggcacc tcgtggtgga ggatcttcgc
      481 ccgtccggtc tgcgtccggg tgtggtagtg gaagagcagc cgcccggtca tcagggtgaa
      541 cggatactcc tcgtcagcaa cctccgcggg cggccgatac tcgataccga agaagtgtcc
      601 aaggccgtct gccgacgaga acttctcgcg gtgcaggatc ggtgttcccg ggtgctccac
      661 ggtcgggcag ggccagtgca gtgactcggg cttttctatc ctctcgtagg agatgccggc
      721 catcgagggg gtgacccggc gcacatcgtt ccagatatcc tccggcgagt tgaaatcgaa
      781 tcccttgagc ccgagtctgt gggcgaggtc gacaaagata tcccagtcgg gccttgcctc
      841 gcccgggggt tccacggcct tgcggacacg gttgacacgc cgctcaccgc tggtaaacgt
      901 cccgtccttc tcggcaaagg agacgcctgg cagaatcacg tcggcatact tcgcagtctc
      961 ggtcatgaat atgtcctgca caaccaggaa atcgagtttc tcaagcgagc gcatcacatg
     1021 gttcgagtcc gggtaggaga cgacggggtt cagcgcaaag atgtacatcg cgcggatcgg
     1081 gtcgccgcac tggttgatct gctcgatcag ggttgcaccg tattcgcctg gaagcccggt
     1141 gacgccccag agctcttcca tccgcttgcg tgtggcgtcg tcctcgcact tctggtagcc
     1201 ggagtagacg ttcgggtaag cacccatgtc acaggcgccc tggacgttgt tctgaccacg
     1261 aagcgggttg acaccggttc ccggcctgcc gacgttgccg gtgagcatgg cgaggttccc
     1321 gagcgacctg acgttgtcag tcccggttgt aagttccgtg atgccgaggc agtagatgat
     1381 cacggcgtta tttgcgctgc cgtagatctg tgcgatctcc ttgatcctct cggttggaac
     1441 accggtgatc gactcgacat ccgcgtactt ctcgacggtc tttttcagtt cctcaaatcc
     1501 agtcgtcctc ttctcgatga actccctgtc gtggagattc tgctggatga tccagtacat
     1561 gatcgagttg atcagggcga tgtttgtcga tgggttatag cgtacgtaat ggtcggccag
     1621 acgcgccgtg ggcgtgtagc gcgggtcgca gacgatgatg gtcttccctg ctttcttggc
     1681 ctgaacaatc cggcgtccgg cgagcgggtg tgcttcaatg gtgttcgccc cgatcatgaa
     1741 gatgacatcg gcgttcagga cgtcctcaaa tgggtttgtc gcggcaccgg agccaaacga
     1801 gagggagagc ccggcaacgg acggcccatg gcagatgcgc gcgcagttgt caacgttgtt
     1861 cgtcttgaag gccaccctgg cgagtttctg catgatgtag cattcctcgt ttggtgtgcg
     1921 gcacgagacc tggaaaccga gggatttcgg cccgtagtta tcgtaggtct ccttgaactt
     1981 cgaggccacg aggtcgagcg cctcatccca ggtcgcttca acaaattcac cattcttctt
     2041 gatgagcggc tttgtcagcc tatcagggct ctggacgaac tcccagcagg tcattccttt
     2101 gggacagagt ttcccctcgt tgatgggggt tctcttgtag ggttcgaccc caacaagctt
     2161 gccgtcgctc accacaaggt tcagcccgca cccaacacca cagtaggggc aggttgtgtg
     2221 aacgtagcgc aatttttcac tggtatcagt cattcacatt ctcccggttg ttgcattcaa
     2281 cgtgggttgc caagcatgcc agacatgctc acaataggtt tactcgactc ggtagtatat
     2341 aagtttaaac atattgtttt aacatacctg tttgttttat gttgtatagt tgattctatg
     2401 cggtctgaat ccactatatc tgacattcac agcaggatcc accggattgc gccacaaaat
     2461 aacatctgac tggtatgata tgatggcact tctgaggagg tatacttgat ccagtttact
     2521 catacaaaat atttacaact ggtgatgatc aaacactcct acgtgcaacc aagactgaat
     2581 catacatggg aagacatcga ggcgcgtctc agggagaagg gtacatctcc ggagttgatt
     2641 gagaacttca ggcgatgcat agagtttcac acataccctg ctccgggtct cctggtcggt
     2701 gtctacatgg tggactacgc cctggaactc ctgaagccgc ctgagggcaa gaagatctac
     2761 gccgtctgcg agactacaaa gtgcctgcct gacgctctgc aggtgatggc tcactgcacg
     2821 accggcaacc atcgcctccg cgtgatccct atcgggaaat ttgctatcac cttaaacggc
     2881 ccggccgacg ccccctacgt gaacggtgtc cgggtcttcg tcgacggcga taagatgcag
     2941 cggtatccta cgtttgccct ctggtacacg aaggacccgc tctttgaccc cagaacacgg
     3001 ggtaccgcgc tgatagacga gatcatcgat gcggggcggg atctcctttc acatgagcag
     3061 gtgcgcgtga aggttcccca gaaattgcca tggaaatctg cggtctgctc catctgcggc
     3121 gagatagttc ccgacaacat gcttgtcaac ggtgtctgca gcgactgccg gtcacaatcc
     3181 tactatgaga aggtcacctg ctgagatttg gcattccaaa cgatggaaga cctgaatccc
     3241 gtcggcacag aaggcatcac atggaactct cacctatagg gctcgtgcat tcaagcatcc
     3301 gatccaggag cgatatgccc atccagggcg tcagtgcgga ggttgagatc tttccagagt
     3361 atgtggccgg gctcgagggt gtcgaggaga actcgcacct gatactggtc tgctggctgc
     3421 accaggcgga caggagcgtc ctgaaggcgg tcgcacggaa gatctcagac gaattaccgg
     3481 agaagggcgt tttctctctc cgttcgccgg cacggccaaa ccccctctcg atctccgtgg
     3541 tgcgactgtt cggtgtacgc gaaggccgat accttctgct tgaacacctc gaccttatcg
     3601 atgggacacc tgttatcgat atcaaacctt accagccggg gtgggactgt gtcttttccg
     3661 cagccgggca tgaccgaacg gaaaagatcc ggaggatgaa gcccggtgac tatcgcgcca
     3721 gcctcatccg cgaggccgtg aactaccacg gagatgtctg ctgtggtgtg gctatcggga
     3781 tgcggatcgc agaagcggcg acgcgcatac tagactgcga cctgcggcac cctggtgtag
     3841 tcgtcgcgcc aggcgccgac ccctgcatcc tcgacgcgct catcggtatc accggcgcga
     3901 cgccgggaaa ccggcggctg aggtgttcgg aaggtagacg ttacgctgtt tcctcttccg
     3961 aaaaggaggt tgtcttccgt ctcctggccg ctcctcagag tgtcgatgat atatttgcag
     4021 ccgaggaaac gtcgctcttt gagtgcacgg ttcacaaccg gccgcaaccg aaataaccag
     4081 taaccaacgg agtccagaga tgacccacag gtacctgaac cttacaccgc tttcaagggc
     4141 gcttgcggtc atgcggcaga agttcccacc gcccgggcgc gcggagagcg tgccgctgca
     4201 actcgccgta ggaagggtaa ccgccgaacc gatctacgcc gtctattccg tcccaatggt
     4261 cgacaccgcg aagtttgatg gctacgcggt gaggagtggc gagacgctcg gagcgcagga
     4321 tcagcggccc ctggcactta ccaattattc ccgcataaac accggcgagg tgctccccca
     4381 ggcgtttgac gccgtgatca tggtcgagga tacctgggaa gaaaacgacc gggtatggat
     4441 ccggaaatct gccgccaggg ggcagaacat ccgtcgggcg ggcgaagata tccgggcggg
     4501 cgaactcgta ctcccgaagg gccaccagat ccggcctttc gacataggtg cagtcgcgac
     4561 ctacggtata accagtgtta tggtgcgggc ggtcagggtc ggtatcatcc cgacggggag
     4621 cgaccttgtc ccgcccggca cgctgccacg tccggggcag acggtcgaga caaacaccct
     4681 gatggcagag gcctatctca gcggtatggg tgccacctgc cgccgctacg gggttgtgcc
     4741 ggacgagccg caccggatcc gggaagcggt ggctgaagcg gtcgccgaga acgatctcgt
     4801 cctcctctca gcagggactt cgacgggcag tcgcgacttc tcccgtgaag ccgtcgccga
     4861 actcggcgag atactcttcc acggcatcgc catgaggcca ggaaaaccgg tgctgctcgc
     4921 gggtctcgac ggaaaaccgg tgttcgggat gccaggatat cctgtttcgg cgcagaccgt
     4981 tctccgggag gttgtcgcga acctgcttgt gtggtggggg cttgcgcccc cgcccgttgc
     5041 ggagatggat gcccggctgg ccagcaggct ggcatcagat ctcgggttcg atgagttcgt
     5101 accggtctcg gtcggccggg tgaacggcac atgctgggca acaccgcacc caaggggtgg
     5161 cggcatccag atggcggtgg tccgggcaaa cggttatctc catgtccctg cctggcgcga
     5221 ggggatcgag gcaggggagg tggtgcgtgt cagtctcacc gctccatctg cctcgatcgc
     5281 caggacaatt gtctgtgtcg ggaagcggga tccagccctc gaaaaactgg gaaaccggtt
     5341 acaggattct ggctacctgc tccactgctg caacgcctcg acgatgggag cggtgctggc
     5401 cctgcgggcg aacacctgtc atgcggcatc gattgttctc ccggatacgg aatccgcctg
     5461 gaacgaccag gtgttccgct atctacccgg cgtgaacctc ctgcggctgc cgctcgccag
     5521 gacggaactc gggatcgcct caactggtga attagatccg gaaaggcttg ctttgctgag
     5581 ggttgccaac cgcccaaaga gccaggcggc acaggttctc cttgatgcct ggcttgagag
     5641 ggaggggatt ggttatcccc atcaggacca ggacctggca gccggtgcaa aggtgcgggt
     5701 ctgcgcgggc gcggaggcgc gggaggcgag gctcagtttt atcccgatcg gttatgagtc
     5761 ttgcaatctg gtgatcaggg gggaggttgc ctcagagggg ggcattgcgg ccctcatccg
     5821 cacaacaaga tcacaggaat tccgcgcgta ccttcggaca atcgggcggg aaccggcagg
     5881 gggcgccggc gatgaaccgc tccctgcccg ggacgctgca cctgctcgaa accaataact
     5941 gttttttgac atgaccctac ctcatgcgag taagcgaaca gacacgtgac caggtcatgg
     6001 cggttctccg gcgactgctg gaggctgccg gcaggaaaga cgccggcggg atcctggcgc
     6061 tcaccgacgc cgatttccgt ggtttctgga gtgtccccgg cgaaaaggtg atcggtaaag
     6121 aggcgttacg ccggcacctt gaacgtggtt ttacgcaggt ggatgcggtc acgcttgagt
     6181 tctccagcgt ccatatcggc gccgagggga cggtcgcctg ggtcatggcc gatctgacct
     6241 gcaacctggt cagaggtgga gccgggcaga ccatggatgg gctggtgacc gcggtccttc
     6301 gggggacggg gcacgcctgg atctttgctc agatacactg ctcattcccg gcgatccggg
     6361 gcaggtgaga cgtcaaaccg gccccggacc aaatgcagga tgttcacgac tcctggtcga
     6421 tcggagcctg cgcaaagaag agcag
//
LOCUS       DAOSZX010000080         6442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10198_length_6442_cov_18.700798, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000080 DAOSZX010000000
VERSION     DAOSZX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10198_length_6442_cov_18.700798"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..2845
                     /locus_tag="PK196_06695"
     CDS             50..2845
                     /locus_tag="PK196_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HON41140.1"
                     /translation="MPRILAVDDEPAFLELTRAYLERDGDIVVETTPSPLQALDMLAE
                     TPYDVIVADYEMPEMNGIALLQEVRRRGLSIPFIIFSGRGREEAIIEALNSGADFYLQ
                     KGGSPSARFAELRNFILQAARRRRAEVEAQRAGDLYRSIFEYTGSATIIIEGDMTISL
                     ANSECERLTGYSRKDIEGKMPFTAFVAPEDRERMENFHRQRRIDPRSAPRTYEFRLID
                     REGRRREIHLTVGIIPGTDRSVASLIDITDRKRFEEELSAAHEEMTAAFEEATASQEA
                     LEAQCREMEDYQATLRGIIDFLPDPTFALDRNGRIIIWNRAMQELTGVRMEQIIGSGA
                     DAISRVIPGLAPPSLAERILRGEVAETPGREVYITSPRTGKEVCLWAKASPLYDAAGK
                     LAGAIESMRDITKSKEMADQIRRRIALEQLVRSISTRFIGLDPSDLDDALQETLELLG
                     SFLGVDRSYILRFSTDLTHAENTNEWCAEGVESQIDVLFNLPEDVITWGLENVRARQT
                     ICIPDLAALPDDEAEIRDFLLDYGISSIILVPIASAAEPLGIIGFETAGRKRRWSDDD
                     ITLLEIVGNLFADLFSRVRAQEQLLENEERFRALIESSHDCYIRVTGTPPVIDYISPS
                     IERLSGYTPEEILGDPDFFERWVHPDDRETLGALLGDLGGCTSRECTLRVRRKDGRYI
                     WIEVSAVPVYGNDGRPVAFHYAIHDIDAWKQAEAALMRANKKLNLMNNIVRHDILNQV
                     TVVLGHLALLREQPLDPAVTGALDRMEVAAEIIKSQIGFTRDYQELGVRAPQWFSVRA
                     AVEEAVKTLRPKGVSVSTDLDDLRIYADPLLSSVFYSLLDNSMRHGRTVTAIRVTAAP
                     DDGGARITWEDDGIGIPAEEKPRIFDRGFGSHTGLGLFLAKEVLAITGITIQETGVPG
                     KGARFEILVPKGAARFG"
     gene            complement(2859..4268)
                     /locus_tag="PK196_06700"
     CDS             complement(2859..4268)
                     /locus_tag="PK196_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HON41141.1"
                     /translation="MTIREIIPVFLLSLALVTVAAGAAVQTDIRCDFPGEVVEAGETA
                     VFDLEITNRGDTGTCLLNYWTYRGTDDWKIRFEDGDREVYRMLIPAGETKTVRLVVET
                     SGRAAVGEYPLRVGIGEGTIWVYVRITKTHSGETGTLEATVVDKDGNVVKGADVGVYD
                     GNTRVEGMLTTAEGKVSIGAPMGTYTVRVSKPGYRSWEKDDVEIRIGQTVDLGIVPLD
                     KENYFAEVLVKTPSRVATVGTNPQYEITVRNIGRNDDTYTLAVPDLPEQWYARFTESS
                     STTEEVPEIYIPAGEEKTIYLEIIPPYSVDVGDYNFTLVAGSSAREYEENLSLRLRGS
                     YDLRTYTKTYRHEINRGETLNFDITITNAGVGGSLTNIGVNMSAPEGWRASVDPATVA
                     SLEPGERATVQVAVVPPADIVAGEYRFIALVRSDQDEQEDEFRVVVNERSYVALLGLL
                     VMVGVAGGLWYIFRKYGRR"
     gene            complement(4274..5374)
                     /locus_tag="PK196_06705"
     CDS             complement(4274..5374)
                     /locus_tag="PK196_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HON41142.1"
                     /translation="MALDRLLNVARKEFSDQITSRRFIIILALFLAISAIGIHTGIEN
                     YNTLLDSYNQQLQYMQDSEAPYAGWMPEKPSIMLVFSAMMSYMVSLGSVLAIATGFDL
                     VSREKETRSLKTLLSHPVYRDEIINGKALGGIAALGFAMVLALAISFAMLLIFSIVPT
                     VEESLAILVFGAVSLLFLLGYFAVALTMSTVAPESGSALIYTLVIFVLVSSLIPVLGM
                     AIGNVFVGDPPQPPQSTPMVVRSDGGGGYFTSTSSQSVVHVGADDPAWQQYEEDRKRY
                     VERQALVYDISYILSPQMNYAVVSMAVTNPRVAPMISSPYTYDYTQASTAETPGLADA
                     LAQVWKNLAALIVFPSVFFAAAYVKFMRMDIR"
     gene            complement(5381..6286)
                     /locus_tag="PK196_06710"
     CDS             complement(5381..6286)
                     /locus_tag="PK196_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41143.1"
                     /translation="MIRTENLTKVYNGKPAVDGLNLEVGEGEIFGFLGPNGAGKSTTI
                     LMLTGMIEPTAGRCFVDGIEVANDPLAVKKIIGYLPEDVGFYGNLTGEQNLDYFARFY
                     DMNAADRKERIAEILRLVHLDGVTQKAGEYSRGMKQRLGLAQALINDPKVLFLDEPTA
                     NLDPEGVREYRDLVTRLAGEGKTVFVSSHILSEVRAVCRTVGLLAAGRLVAQGTIDEV
                     ARTLASPGGETFRIVVETAEDLPDLSDPDIIEFERDGNRAVIQAKSDIRERLAAAIAE
                     EGLHIREIRLEEPSIEDAFMALYRG"
BASE COUNT         1240 a         1960 c         1988 g         1254 t
ORIGIN      
        1 ctaactcaaa aaggcttatc gtttctgcta ctgagtcatt gtttaactta tgcccaggat
       61 acttgcagtc gatgacgaac cggcgtttct cgaactgacc cgcgcatacc tggagcggga
      121 tggagatata gtggtcgaga caacgccatc gccgctgcag gctctcgata tgctcgcaga
      181 gaccccatac gatgtcatcg tcgccgacta cgagatgccg gagatgaacg gcatcgcgct
      241 cctccaggag gttcggcgca gaggactctc gataccgttc atcatcttct ccggccgcgg
      301 gcgtgaggag gcaatcatcg aggcgctcaa cagcggcgca gacttctacc tccagaaagg
      361 cggcagtcct tccgccaggt ttgccgaact ccgcaacttc atcctgcagg cggccaggcg
      421 acggcgtgcc gaggtggagg cgcagcgtgc cggcgacctc taccggagca tctttgagta
      481 caccggctct gccaccatca taattgaagg agatatgact atctcgcttg caaacagtga
      541 gtgcgaacgg ctcaccggct actcccgtaa ggatatcgag gggaagatgc cctttacggc
      601 cttcgtcgcg cctgaagacc gcgaacgcat ggagaacttc caccgccagc gaaggataga
      661 cccgagatcg gcgccgcgga catacgagtt caggttgatc gaccgcgagg ggcggcggag
      721 agagatccac ctgacggtcg ggatcatccc gggaaccgat cgctccgttg catctctgat
      781 cgatatcacc gaccggaaac ggttcgagga agaactgagc gcggcgcacg aagagatgac
      841 cgcggcgttc gaggaggcga cggcaagcca ggaggcgctt gaggcgcagt gccgcgagat
      901 ggaagactac caggcaaccc tccgcggcat catcgacttt ctccccgacc ccacgtttgc
      961 acttgaccgt aacggcagga tcatcatctg gaaccgggcg atgcaggaac tgaccggtgt
     1021 cagaatggag cagataatcg gttccggggc ggatgccata tcacgtgtga tcccggggct
     1081 ggcaccgccg tcgcttgcgg agaggatcct cagaggtgag gttgcagaga ctcccggccg
     1141 cgaggtctac atcacctccc cgaggacagg gaaagaggtc tgcctctggg cgaaggcatc
     1201 gccgctctac gacgcagcag ggaaactcgc cggcgcgatc gagtcgatgc gcgacatcac
     1261 aaagtcaaaa gagatggcag accagatccg gcggcgtatc gccctcgagc aactggtgag
     1321 gtcgatctcc acccggttca tcggcctcga cccctctgac ctcgatgacg ccctccagga
     1381 gacgctggag ttgctcgggt cgttcctcgg cgtcgaccgg agttacatcc tccgcttctc
     1441 aacagacctc acccatgccg agaacaccaa cgagtggtgc gccgaagggg tcgaatcgca
     1501 gatcgacgtc ctttttaacc tcccggagga cgtgatcacc tgggggctcg agaacgtgag
     1561 ggcgcgccag acgatctgca tcccggatct tgccgcgctc cccgatgacg aggcggagat
     1621 acgcgacttc ctgctggatt acgggatctc ctcgatcatc cttgtgccga tcgcaagcgc
     1681 ggcggagccc ctcggcatca tcgggtttga gaccgccggg agaaaacgaa gatggtctga
     1741 tgacgacata acgctgcttg agatcgtcgg caacctcttt gccgacctct tctccagggt
     1801 ccgtgcacag gagcaactcc ttgagaatga ggagcggttc agggcgctta tagagagttc
     1861 gcatgactgt tacatccggg tgaccgggac accgccggtg atcgactaca tcagcccctc
     1921 aatcgagaga ctgagcggct acaccccgga agagatcctg ggcgacccgg acttcttcga
     1981 gcggtgggtc cacccggatg accgggagac gctcggggcg ctgcttggag acctgggcgg
     2041 ttgcacctcc agggagtgca cgctccgggt tcggcggaaa gacggccgct acatatggat
     2101 agaggtctca gcggtccccg tctacggtaa cgacggccgg ccggttgcgt ttcattacgc
     2161 gatccacgac atcgacgcct ggaaacaggc cgaggcagcg ctcatgcggg ccaacaagaa
     2221 actcaacctg atgaacaaca tcgtccgcca cgacatcttg aaccaggtca cggtggtgct
     2281 ggggcatctt gccctcctgc gcgaacagcc gctcgatccc gccgtcacag gtgctctcga
     2341 ccgtatggag gttgccgcgg agatcatcaa gtcgcagatc gggttcaccc gggattacca
     2401 ggaactcggc gtccgcgcac cacaatggtt ctccgtcagg gcggcggtcg aggaggcggt
     2461 aaaaacgctc cggccgaagg gggtcagcgt ctcaaccgat ctcgatgacc tccgcatcta
     2521 cgccgacccg ctcctctcct cggtctttta tagcctcctc gataacagca tgcggcacgg
     2581 caggaccgtg accgcgatcc gggtcacggc cgcaccggac gacggcggtg cacggatcac
     2641 ctgggaggat gacggcatcg gcatccccgc cgaggagaaa ccgcggatat tcgatcgcgg
     2701 tttcgggagc cacaccgggc tcgggctctt cctggcaaaa gaggtgcttg cgatcacggg
     2761 gatcacgatc caggagaccg gggttccagg aaaaggggcg cggttcgaga tcctggtccc
     2821 gaaaggcgcc gcacggtttg ggtgagaccg aacggatctc accgccgccc gtacttcctg
     2881 aagatgtacc agagcccgcc ggcaaccccc accatcacca gcagacccag cagggcaacg
     2941 taagaccgtt cgttcaccac gacccggaac tcgtcctcct gctcgtcctg gtcgctcctg
     3001 acaagggcta tgaacctgta ctccccggcg actatatccg ccggcgggac cacggcaacc
     3061 tgcaccgtcg cccgttctcc gggctcaagg ctcgcgaccg ttgccggatc gacgcttgcg
     3121 cgccagcctt ccggcgcgct catgttgacg cctatgtttg tcaggctgcc gccaacacct
     3181 gcgtttgtga tcgtgatatc gaagttgagg gtctcgccgc ggttgatctc gtggcggtag
     3241 gtctttgtgt aggtccgaag gtcgtaggag ccgcggagcc ggaggctcag gttctcctcg
     3301 tactcgcggg ccgacgaacc tgccacgagc gtgaagttgt agtcgcccac atccaccgag
     3361 tagggcggga ttatctcaag gtagatggtc ttctcctcgc cggcggggat gtagatctcc
     3421 gggacctcct cggtggtgct gctgctctcc gtgaatcggg cataccactg ctccgggagg
     3481 tcggggacgg caagggtgta ggtatcgtcg ttcctcccga tgttccttac ggttatctca
     3541 tactgcgggt ttgtgccgac cgtcgccaca cgtgaggggg tcttcaccag gacctcggca
     3601 aagtagttct ccttatcgag cgggactatc ccgaggtcga ccgtctggcc gatccggatc
     3661 tcgacgtcat ccttctccca ggagcggtag ccgggtttgc tgaccctgac ggtgtaggtg
     3721 cccatgggtg cgccgatgct caccttcccc tcggcggtcg tgagcatccc ttcgaccctg
     3781 gtattgccgt cgtatacgcc gacgtcagca cccttgacga cgtttccatc tttatcgacc
     3841 acagtcgcct ccagggtgcc ggtctcgccg ctgtgggtct ttgtgatcct gacgtagacc
     3901 cagatcgtgc cttcgccgat acctacccgg agggggtact ccccgaccgc tgcacgcccg
     3961 gaggtctcaa caaccaggcg gacggtcttc gtctcgccgg cggggatgag catccggtag
     4021 acctcgcggt caccgtcctc gaaccggatc ttccagtcgt cggtcccccg gtatgtccag
     4081 tagttgagga ggcacgtccc ggtatccccg cggttcgtga tctcgagatc gaagaccgcg
     4141 gtctcaccgg cctcgaccac ctcgccgggg aagtcgcacc ggatatctgt ctggacggct
     4201 gcgcccgccg caaccgtgac cagggcgagg gagaggagga agacaggtat gatctctcgg
     4261 atggtcaatc gtatcaccgt atatccatcc tcatgaactt cacataggcc gcggcgaaga
     4321 agaccgatgg gaagactatg agggcggcga ggttcttcca gacctgggca agggcatcgg
     4381 caaggcccgg ggtctccgcg gtggaggcct gggtgtagtc gtaggtgtag ggagaggaga
     4441 tcatcggagc gaccctcggg tttgtgaccg ccatggaaac cacggcgtag ttcatctgcg
     4501 gcgagagtat gtatgatatg tcgtatacga gggcctgccg ttcgacatac cgtttcctgt
     4561 cctcttcata ctgctgccag gcggggtcat cggccccaac atggaccaca ctctgggagg
     4621 acgtgctggt gaagtatcca cccccgccgt cgctcctgac caccatcggt gttgactgcg
     4681 gcggttgggg cgggtcgccc acaaagacgt tcccgatcgc catcccgagg acggggatga
     4741 gcgaggagac caacacaaag atcacaaggg tgtagatcag agcgctcccg ctctccgggg
     4801 cgaccgtcga catcgtgagc gcgacggcga agtagccaag caggaataga agcgagacgg
     4861 ctccaaagac caggatggcg agagactcct caaccgtcgg gacgatcgag aatatgagga
     4921 gcatcgcaaa cgatatggcg agcgcgagca ccatcgcaaa cccgagcgcg gcgatgccgc
     4981 cgagcgcctt gccgttgatg atctcgtcgc ggtagaccgg gtgtgagagg agtgtcttta
     5041 gcgatcgggt ctccttctct cttgatacca ggtcgaaacc ggttgcgatg gcaaggacgc
     5101 tgccaagcga gaccatgtaa ctcatcatgg cgctgaagac gagcatgatc gagggtttct
     5161 cgggcatcca gccggcgtat ggcgcttcag agtcctgcat gtactggagc tgctggttgt
     5221 aggagtcgag gagggtgttg tagttctcga tcccggtgtg gatgccgatc gccgatatcg
     5281 ccaggaagag cgccaggatg atgatgaacc gtctgctggt gatctggtcg gagaactctt
     5341 ttcgtgcgac gttgagcagt cgatcgagtg ccatgctgta tcacccccgg tagagcgcca
     5401 tgaaggcgtc ctcgatgcta ggttcttcga gccgtatctc ccggatatgc agcccctctt
     5461 cggcgatggc cgcggcaagc ctctcccgga tatcgctctt tgcctggatg accgcgcggt
     5521 tcccgtcccg ctcgaactcg atgatatcgg ggtcgctgag gtcaggcagg tcttctgcgg
     5581 tctcgacgac gatccggaac gtctcgccgc cgggggaggc aagcgtccgg gcgacttcgt
     5641 cgatggttcc ctgcgcaaca agccttcctg ccgcaagcag ccctaccgtc cggcagaccg
     5701 cccggacctc agagaggatg tgggaggaga caaagacagt ctttccctct ccggcaagcc
     5761 gcgtgacgag gtcgcggtac tccctgacgc cctcgggatc gaggtttgcc gtgggttcgt
     5821 cgaggaagag cactttcgga tcgttgatca gcgcctgggc aagtccgagc cgctgtttca
     5881 tcccgcggga gtactcccct gccttctggg tgacgccgtc gaggtggacg agtctgagga
     5941 tctcggcgat ccgttccttc cggtctgctg cgttcatgtc gtagaaccgg gcgaagtagt
     6001 ccaggttctg ctcgccggtc aggttcccgt agaacccgac atcttcgggg aggtagccta
     6061 tgatcttctt cacggcgagc gggtcgtttg cgacctcgat cccgtcgaca aaacaccggc
     6121 cggcggttgg ctcgatcatg ccggtgagca tcaggatcgt tgtgctcttc ccggcaccgt
     6181 tcgggccgag gaaaccgaag atctcgcctt ccccgacctc caggttcagg ccgtctacgg
     6241 ccggttttcc gttatacacc tttgtaaggt tctctgttct tatcatttca cactccgcca
     6301 tgaccgtttc ctgcggcgtc ttgaggcaag cttcgacctc agtctattaa cgttttctct
     6361 gtcaatcatc tacacgtcgt gcggccaatc aagatcagcg aacctggaat atggactttc
     6421 catcatttcg gggcatgggg at
//
LOCUS       DAOSZX010000081         6329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10417_length_6329_cov_19.218999, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000081 DAOSZX010000000
VERSION     DAOSZX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10417_length_6329_cov_19.218999"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1935
                     /gene="polX"
                     /locus_tag="PK196_06715"
     CDS             211..1935
                     /gene="polX"
                     /locus_tag="PK196_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HON41144.1"
                     /translation="MAGVTNLEVAAILREVADLLEIKGVRFKPQAYRRAALAVETMPE
                     DIADVAAAGKLEEIPGVGKAIAKKIQEIVETGKLGYLESLRQELPEGVLHLVGISGIG
                     PKKAIALSQALGVRTVDDLEAAAKAGRIRGLPGFGEKTERNILESIQMARRARERRLL
                     GQILPLARDIERRLAAHPSVRRVSLAGSIRRRKETVGDVDILAASTGPAEVMEVFASL
                     PGVERVLARGLTKTSVVLSAGIQVDLRVVEDTQFGAALQYFTGSKEHNIALRRLAIAR
                     NWRLNEYGLVDLSTGRQIAGEDEEGVYQALGLPWIEPELRENRGEIEAARAGRLPDLV
                     GYDEIRGDLHVHTTWSEGAHSIEEMAEAARALGYEYIAICDHAEALRIARGLSPERIA
                     EQVREIERINRESGSGFTVLAGIECNIGPDGGLDLPDSVLADLDVVVASVHSGLKQPE
                     EEMTKRLITAMHNDNVDIIGHPTGRILLEREPSRIDLAAVFEAAANLGVMLEINAYPS
                     RLDLSDVNARAAREAGVRLSLGSDAHSRENLRFMEFGVATARRGWLSADDIANTRPLP
                     ELKKILRR"
     gene            complement(2059..2496)
                     /locus_tag="PK196_06720"
     CDS             complement(2059..2496)
                     /locus_tag="PK196_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON41145.1"
                     /translation="MAQAREGDTVRVHYTGRLEDGTVFDTSENQEPLEFTIGNGEVIP
                     GFERAVAGMEPGEVKTATIQPKDAYGPRLDDMTITIDRDRFPADIEPEPGQQLQIQQP
                     DGWAAIVTVTRVTESGVTLDANHPLAGQPLTFEIRLIEIVETE"
     gene            complement(2537..3178)
                     /locus_tag="PK196_06725"
     CDS             complement(2537..3178)
                     /locus_tag="PK196_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HON41146.1"
                     /translation="MHRKDAPLLTTDDGLTFRRMPPEADAIYLVGEYRFDDIQKDDLV
                     IDIGANVGGFCIRAARRSDHVLAVEPVLAEALRENIAMNRLNVTVVEGALGTGECTRI
                     TWGGRTVCLTTRPLSDYIGMAGGCDFLKCDCEGAEWTIQPSDLDGVRRIEMELHAPPI
                     GGPVNRALLDYIGRHYEFEIDRTPISGTQGVMGILHAARKTGRRRLTPGHRHA"
     gene            complement(3240..4817)
                     /locus_tag="PK196_06730"
     CDS             complement(3240..4817)
                     /locus_tag="PK196_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON41147.1"
                     /translation="MDKNIRFQDFNISPETLRAIEEMGFEEPTPIQVSAIPALLEGRD
                     VTGQAQTGTGKTAAFGIPAIERIDPRSRKTQVLVLSPTRELAIQIAEEFARLAACHDG
                     ISILPIYGGQPIERQFRALERGAQIVVGTPGRILDHLDRGTLSFDSVRLVVLDEADQM
                     LDMGFRDDIVAILDETPADRQTVLFSATLPKPILEISKRFQKDPAFISVARREVTVPQ
                     IEQLYLEVRSRDRLEILTRVLDIYDPDLTLIFTNTKKGVDELTSHLQARGYFAEGLHG
                     DMKQVLRDRVMAKFRSGTIDILVATDVAARGIDVDNVDLVINYDVPQDIDYYIHRIGR
                     TARAGRTGRAITFVGPKDYFKLRMIQDYTRIKIARIPLPTPGDVEESRTRKLIERVKH
                     TINEGDLERYINIVERIVAEDYTSLEVAAALLKQEIAGAAQGQPQDLRPGTALLRIPA
                     GRLHQIRPKDIVGALAGETGIPGNAIGAINIYETHSTVEVPERLAGQVMEGMKGKSIG
                     RVRLDQPIEIAGTSSGQ"
     gene            complement(5172..5705)
                     /locus_tag="PK196_06735"
     CDS             complement(5172..5705)
                     /locus_tag="PK196_06735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HON41148.1"
                     /translation="MKPTRIRTERLDMIPATLDTLRSDREKLARLLDAAVPGSWPPPL
                     LDDDTRDAFTLMIAEKGDPFFAAWYWVRDDPAEGGRVLIGCAAIATPPTADDGGDTVL
                     IGYSVLDEFQHRGYATEAVRHLIPLIFSVPGIRRITATTYPELGAAIRVLEKNGFSPA
                     GEGFEEGTIAYVLEKDD"
     gene            complement(5732..>6329)
                     /locus_tag="PK196_06740"
     CDS             complement(5732..>6329)
                     /locus_tag="PK196_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON41149.1"
                     /translation="PHMPPMHPHLRTERLDLIPATLETLRSGRQTLASLLNAAVPPSW
                     PPPLLDDEALAGFIRMMTEKTDPRFAVYYWVRNDPAAGERLLIGLGGIASAPAPGTVF
                     IGYTVVRECQGRGYATEAVRQIVSAAFSLPGIRWIMATTHPDHAASIRVLEKNGFRRA
                     GRGFEKGTIAFLLEKEEHPPGLTRPGTPSDSTSDNNLP"
BASE COUNT         1058 a         1810 c         2186 g         1275 t
ORIGIN      
        1 gggaaaagga actttccctc catctctcca atctcccggg aatggcgtat cgcaaaaccc
       61 cgcggccacc cccccaggat caccataacg ttcaagtaat ccgcaggctg aagactccct
      121 taaccgtgaa actcgcaact ctcataacgc tcgcgtccac cggccgcccc gccatcgcag
      181 aaagagcata tctgccgggg gtgtgaccac ctggcgggtg ttaccaacct cgaggtcgcg
      241 gcgatccttc gcgaggttgc cgatctgctc gagatcaagg gtgtccggtt caaaccccag
      301 gcctaccgca gggcggcgct ggcggtcgag acgatgccgg aggatatcgc cgacgtggcg
      361 gcggcaggga aacttgaaga gatcccgggc gtgggaaagg cgattgcaaa aaagatccag
      421 gagatcgtcg agaccggcaa actcggctac ctcgagagcc tgcgccagga actcccggag
      481 ggggtgcttc acctggtcgg gatcagcggg atcgggccga agaaggcgat tgccctctct
      541 caggcgctcg gtgtccggac ggtcgatgat cttgaggcgg cggcgaaggc cggccggatc
      601 cgcggcctcc cggggtttgg cgagaagacc gaacgaaaca tccttgagag cattcagatg
      661 gcccggcggg cccgggagcg ccggctcctc gggcagatcc tgcccctcgc tcgtgatatc
      721 gagcgtcggc tggcggcgca tccttcggtt cgcagggtga gccttgccgg gtcgatccgc
      781 cggcgaaaag agactgtcgg ggacgtcgat atcctggcgg cctcgaccgg tcccgcggag
      841 gttatggagg tctttgccag cctccccggg gttgagcgcg tcctcgcccg cgggttgaca
      901 aagacatcgg tggtgctctc ggccggcatc caggtcgatc tccgcgtggt ggaggatacg
      961 cagtttggtg cggcgctgca gtacttcacc ggctcaaagg agcacaacat agcgctccgg
     1021 aggcttgcca tcgccaggaa ctggcggttg aacgagtacg ggcttgtgga tctctcaacc
     1081 ggccgtcaga tcgccggcga ggacgaggag ggggtctacc aggccctcgg tctcccctgg
     1141 atcgagccgg aactgaggga gaaccggggc gagatcgagg ctgccagggc cgggagactg
     1201 cccgacctgg tcggctacga cgagatcagg ggcgacctcc acgttcacac cacctggagc
     1261 gagggcgctc actccatcga ggagatggcg gaggctgccc gggcgctcgg gtatgaatac
     1321 atcgcgatct gcgaccacgc ggaggcgctc cgtatagccc gcggtctctc gccggaacgt
     1381 attgccgaac aggtgcgaga gatcgagcgg atcaacaggg agagcggcag cgggttcacc
     1441 gttctcgcgg gtatcgagtg caacataggc cccgacggcg gtcttgacct ccccgacagc
     1501 gtcctcgcag atctcgatgt ggtggtggca agcgtccact cggggctgaa gcagccggag
     1561 gaggagatga caaaacggct gatcacggcg atgcataacg ataatgtcga tatcataggt
     1621 cacccgacgg gccggatcct gcttgagcgc gagccctcca ggatcgatct cgctgcggtc
     1681 tttgaggcgg cggcaaacct cggggttatg ctggagataa acgcttaccc ttccagactg
     1741 gatctctccg acgtcaacgc ccgtgcggcc cgcgaggcgg gtgtccggct atcgctcggc
     1801 tcagatgctc acagccgcga aaacctgcgg ttcatggagt tcggggttgc aacggcccgg
     1861 cggggatggc tctcggcgga tgatatcgcc aatacccggc cgctaccaga gttaaaaaag
     1921 atcctacggc ggtgagacct cgctggactt ttcggcgcag cgtcggtaag aaaactgaga
     1981 aatgagggta tctgcatact ctctccggat cttgaagagg cagagcggga agaccccctc
     2041 gtgcaaaggt ggggtagttc actccgtctc gactatctcg ataagccgga tctcaaacgt
     2101 caggggctgc cctgccaggg ggtggttggc gtcgagggtc acacccgatt ccgttacccg
     2161 ggtgacggtc acgatggccg cccagccgtc gggctgctgg atctgtaact gctgccccgg
     2221 ttcgggctcg atatcagcag ggaaccggtc ccggtcgatg gttatggtca tatcgtcgag
     2281 gcgggggccg tatgcatctt tgggctggat tgtggcggtc tttacctctc ccggttccat
     2341 gccggcgacg gcgcgctcaa acccggggat gacctctccg ttgccgatgg taaattcgag
     2401 gggctcctgg ttctcggatg tgtcaaagac cgtcccatct tccagtctcc cggtgtaatg
     2461 caccctgacg gtgtcgcctt ctcttgcctg tgccatactt tctatcacca ccgggagcgt
     2521 tgtcgccgat ctctatttat gcgtgccggt gaccgggtgt caggcgccgc ctcccggttt
     2581 ttcgggcggc gtgcaggatc cccatcacac cctgtgtgcc gctgatggga gtccgatcga
     2641 tctcaaactc gtagtgtctg ccgatgtagt ccaggagcgc ccggttcacc ggccccccga
     2701 tggggggtgc gtggagttcc atctcgatcc ggcggacacc atcaaggtcg gacggctgga
     2761 tggtccactc cgcaccctcg cagtcgcact tgaggaagtc gcagccgccg gccatgccga
     2821 tgtagtcgga taggggtctg gtggtcagac agaccgtacg cccgccccag gtgatccggg
     2881 tgcactcgcc ggtgccgagc gctccctcca ccaccgtgac gttgagacgg ttcatggcga
     2941 tgttctcgcg gagggcttct gcaaggacgg gttcgacggc caggacgtgg tctgagcgcc
     3001 tggcggcccg gatgcagaac ccgccgacgt ttgccccgat gtcaatgacg agatcatctt
     3061 tctggatgtc gtcaaaacgg tactctccga cgaggtagat ggcgtcggcc tctggcggca
     3121 tcctcctgaa cgtcaggcca tcgtctgtgg tgaggagcgg ggcgtctttt cggtgcatcg
     3181 caacggttca tttagggttt gggtttttcc agggtgccgg ccgggtgggg gagggttgat
     3241 cactgcccgc tcgatgttcc cgcgatctcg atgggctggt ccagtctgac cctgccgatg
     3301 ctctttccct tcatcccctc catgacctgg ccggcgagcc tctcggggac ctcgacggtt
     3361 gagtgggtct cgtagatgtt gatagcccct atggcgttcc cggggatccc ggtctcgccg
     3421 gcgagcgccc cgacgatgtc ttttggccgg atctggtgca gtcttcccgc gggtatccgg
     3481 aggagggcgg ttccgggacg gaggtcctgc ggctgaccct gggctgcccc ggcgatctcc
     3541 tgtttaagga gggcggcggc gacctcgagg gaggtgtagt cctcggcgac gatccgttcg
     3601 acgatattga tgtagcgttc gagatcgcct tcgtttatgg tgtgtttcac cctctcgatg
     3661 agtttgcgcg tccggctctc ttcgacgtcg ccgggggtgg gaagcgggat gcgtgcgatc
     3721 ttgatcctgg tgtagtcctg gatcatccgg agtttgaagt agtctttcgg gccgacgaag
     3781 gtgatggcac ggccggtcct ccctgcccgt gccgtcctgc cgatgcggtg gatgtagtag
     3841 tcgatgtcct gggggacgtc gtagttgatg accaggtcga cgttgtcgac gtcgatcccc
     3901 ctggcggcga cgtccgtcgc gacaaggatg tcgatcgttc cggaacggaa ctttgccatg
     3961 acgcggtcac gcagcacctg tttcatgtcg ccgtggagcc cttcggcaaa gtagccccgg
     4021 gcctggaggt gggaggtgag ttcgtcgacg ccttttttgg tgtttgtgaa gatcagggtg
     4081 aggtcggggt cgtatatgtc gaggacgcgt gtgaggatct cgagcctgtc tcggctccgc
     4141 acctcaaggt agagctgctc gatctggggg acggtcacct cccggcgtgc gaccgatatg
     4201 aacgcggggt ctttctggaa ccgttttgag atctcaagta tgggttttgg gagggtggcc
     4261 gagaagagga cggtctggcg gtctgccggg gtctcgtcca ggatggccac gatatcgtcc
     4321 ctgaaaccca tatcgagcat ctggtcggct tcgtccagga cgacgagcct tacggagtca
     4381 aaggagagtg tgccgcggtc gaggtggtcg aggatccggc ccggcgttcc gacaacgatc
     4441 tgggcgccgc gttccagcgc ccggaactgc cgttcgattg gctggccgcc gtagatcggg
     4501 aggatgctga tcccatcgtg gcaggctgcc agccgggcga actcttctgc gatctggatg
     4561 gcgagttcgc gggtggggga gaggaccaga acctgggtct tccggctccg ggggtcgatg
     4621 cgctcgatcg cggggatgcc aaacgccgct gtcttgccgg tgcctgtctg ggcctggccg
     4681 gtgacgtcgc ggccttcgag caatgccggg atggcgctga cctggatggg ggtcggctct
     4741 tcaaacccca tctcttcgat ggcgcggagg gtttctggcg agatattgaa gtcctggaat
     4801 ctgatattct tatccatttg agagatattg gtcttctgat ctctttatat gtcgcatcgc
     4861 cacacatttc atctgatcag gtgggcatca aaccgatacc cgccacggtt tggtgaacct
     4921 ctccatggac ggattccggg gggatggcgg catcgccgtg cactgccggc ccatccggat
     4981 ccggggcggc cggggttttt gtgagatgcc gctcccggga gattggagag acgggcgatt
     5041 ctatcaaacc atgagtgaac cggtgctgcc ggggagggtg gaggggttat gccacaggac
     5101 cggtgatctg atggcctcag ccggtgggaa ggaactgttg cgatcctgta tgcgggaaaa
     5161 aggagcaggg ctcagtcgtc cttttccaga acgtatgcga tcgttccctc ttcaaaccct
     5221 tctccggcgg gggaaaaccc gttcttctcg agcacccgga tggctgcgcc gagttcgggg
     5281 taggttgttg cggttatccg cctgatgccg gggacggaga agatcaaggg gatgaggtgg
     5341 cggacggcct cggtcgcgta gccacggtgc tggaactcgt cgaggacgga gtagccgatg
     5401 aggacggtgt ctccgccgtc atcggccgtt ggcggggtgg cgatcgcggc acagcctatg
     5461 aggactctgc cgccctctgc ggggtcgtcc cgcacccagt accaggcggc gaagaagggg
     5521 tcgcctttct cggcgatcat gagggtgaaa gcgtcgcggg tgtcgtcgtc gaggagcggc
     5581 ggcggccagg atcctggaac ggcggcatcg agcagcctgg cgagtttctc gcggtcactc
     5641 ctgagggtat cgagggttgc cgggatcatg tccaggcgtt cggtgcgtat gcgggtgggt
     5701 ttcattcgtt tcacaaccag agtagggtta tctatgggag gttattatca cttgtggaat
     5761 ccgagggggt tcccgggcgg gtcagacccg gcgggtgctc ctccttctca aggaggaagg
     5821 caatcgttcc cttctcaaac cctcttcctg cgcggcgaaa cccgttcttc tcgagcaccc
     5881 ggattgacgc agcgtggtcg gggtgtgtgg ttgccatgat ccagcggatg ccggggaggg
     5941 agaaggcggc actgacgatc tggcggacgg cctcggttgc atacccccgg ccctggcact
     6001 ccctgaccac ggtgtagccg atgaagaccg tgccgggggc gggtgcagag gcgatcccgc
     6061 cgaggccgat gaggagacgt tctccggcgg cggggtcgtt tcgcacccag tagtagacgg
     6121 caaagcgggg gtcggtcttc tctgtcatca tcctgatgaa cccggcgagc gcctcgtcgt
     6181 ccaggagcgg cggcggccag gatgggggaa cggcggcatt gaggaggctg gcaagggtct
     6241 ggcggccgct cctgagggtc tcaagggttg ccgggatcag gtcaaggcgt tcggtgcgga
     6301 ggtgggggtg catggggggc atgtgcggg
//
LOCUS       DAOSZX010000082         6318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10436_length_6318_cov_21.619671, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000082 DAOSZX010000000
VERSION     DAOSZX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_6318_cov_21.619671"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK196_06745"
     CDS             <1..278
                     /locus_tag="PK196_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON41150.1"
                     /translation="SAGTNFIQIDPRNTTQMCSNCGSIVKKTLSERVHECPYCGFVAD
                     RDYNAAVNIHRVGMEQPFEPVEPRPLHHISVVQVLSMKQEAPPFRAG"
     gene            380..1018
                     /locus_tag="PK196_06750"
     CDS             380..1018
                     /locus_tag="PK196_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HON41151.1"
                     /translation="MGIKEWVVPQDKVFFDLFDRMAQTVVSAADMLVEIVENFAKIEN
                     VEEQCRAMKEIEHQGDEITHEIYEQLNLTFITPLEPEEISRLASALDDILDYIDGTMQ
                     QMYSYGITETDNSMVELAKLVQLSVIEIEKAVNGIRTIKNPTQIEERCIEVNRLENIA
                     DNVLGRAIMDLFKTKDAITIIKLKDIYENLEIATDKCEDAANVLSDIAIRHT"
     gene            1024..>2151
                     /locus_tag="PK196_06755"
     CDS             1024..>2151
                     /locus_tag="PK196_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HON41152.1"
                     /translation="MDPIIVLGIILALLFNFANGLNDAANSIATIIATKALTPLQAVL
                     LAGVFNLLGPLFFTTAIAATIGKGIVDPAFLTPLLILMAMVGAVLWVLITSFFGIPVS
                     SSHALIGGLVGAGIAAAGTGAVLWPPVALVVQTVLYILIGVFLGAAVAAVATYRRDEF
                     KLRNLTIGGLIGVTVIIPLAIATGFLKIGGLLAVIIFIVISPMLGFLSAFVLATLITS
                     IFRNYPPRRLSSLFKNLQIFSGSFQAIGHGSNDAQNAMGIITAMLLAGGLITEFHVPL
                     WVIFASSLAISLGTLFGGWRVIDRMANKITRIRPYQGFSASTAAGGVLSLMTAFGVPV
                     STTHVTTGAIMGVGTTRGYSAVKWGVVREILIAWILTIPASA"
     assembly_gap    2152..2251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2252..2715)
                     /gene="nth"
                     /locus_tag="PK196_06760"
     CDS             complement(<2252..2715)
                     /gene="nth"
                     /locus_tag="PK196_06760"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HON41153.1"
                     /translation="MNRKTACEVYRRLLARYPIVDGRRHFIEFHNPFEALILTILSAQ
                     TTDRAVNAIRDELFSRYPTPEALASAEPEEVEPLIRSIGFHHMKARYIVGAARKIVSG
                     FGGEVPKTMEELLSLPGVGRKTANIVLSHAFGINVGIAVDTHVRRVSKRLGFT"
     gene            complement(2722..2796)
                     /locus_tag="PK196_06765"
     tRNA            complement(2722..2796)
                     /locus_tag="PK196_06765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2758..2760),aa:Pro,seq:tgg)
     gene            complement(2969..4150)
                     /locus_tag="PK196_06770"
     CDS             complement(2969..4150)
                     /locus_tag="PK196_06770"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HON41154.1"
                     /translation="MYLIGEALVGDGAELAHVDLIMGNKEGPVGQAFANALSQLSKGH
                     TPLLAVVRPNLPAKPSTIIIPKVTLKKEYQVNQIFGPVQSAVAKAVADLVEEGAFEGI
                     DIEDIVIMASVFVHPTAEDYNKLYRFNYGATKLAIRRALDRFPDVDTLLHEKDRATHA
                     VMGFKVQRLWNPPYLQVAMDLVDRNHMRKVLEELPESDHLILEAGTPLIKKFGLSIIS
                     EIHEIRPGSFIVADLKTLDTGNLEARMAADAGADAVVISGLAPLSTIEKAIQEARKTG
                     IYSVLDTLNVSDPVAVIGQLKVKPDVVELHRAIDIEDTDYAWGDIPAIRKAGGERMLI
                     ATAGGIRQHVVKEALRAGADILVVGRAITASKNIKHAAEEFLEELNTEEVDQFRIMTD
                     F"
     gene            complement(4458..4611)
                     /locus_tag="PK196_06775"
     tRNA            complement(join(4458..4493,4574..4611))
                     /locus_tag="PK196_06775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4575..4577),aa:Trp,seq:cca)
     gene            4706..>6318
                     /locus_tag="PK196_06780"
     CDS             4706..>6318
                     /locus_tag="PK196_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON41155.1"
                     /translation="MVEGSYACGTSQIPLLGITIGEMLNRIAAAHPDNEALVAVHQGI
                     RWTYAEFLDRVNTLARALMALDVERGDRVAIWALNYAEWVLVQFATAKIGAIMVNINP
                     AYRTYEFEYAMKQSEVQTLFIQGRFKTSDYVGMFYESCPEAFEAKPGRIDSDRFPFLK
                     NVIFLGDIPYNGMYTWDDLMKMADLISPDELREREESLDFDDPVNIQYTSGTTGFPKG
                     VVLTHHNVLNNGFFIGEGMEFTHHDRLCIPVPFYHCFGMVLSNMASVTHGATMVLPSP
                     VFDAEAVLKAVQDERCTALHGVPTMFIAELAHPNFENYRLDSLRTGIMAGSPCPTEVM
                     REVNKRMHISEIVIVYGQTETSPGITMTTTDDPLERRVSTVGRPFPHTEVKIIDPNTK
                     RIVPRGETGEICARGYCVMRCYYNNPNATRATIDENGWNHTGDLGVMDEENYVKIVGR
                     LKDMVIRGGENIYPREIEEFLHTHPKIADAYVIGVPDRKYGEELMAWIRVDNGVRLTE
                     EDVREFCRGRIAHFKIPRYIKFVDDFPMTV"
BASE COUNT         1363 a         1733 c         1745 g         1377 t
ORIGIN      
        1 aaagtgctgg tacgaacttc attcaaatcg atccccggaa tacgacgcag atgtgttcga
       61 actgcggaag catcgtgaaa aagacgctct ccgagagagt ccacgaatgc ccctactgcg
      121 ggtttgttgc cgatagagat tacaatgctg cggtgaatat tcaccgtgtg gggatggaac
      181 agccctttga gcctgtggag ccaagacctc tacatcacat ctccgtggtg caagtgttgt
      241 ccatgaagca ggaagccccg ccattcaggg cggggtagtt cacgagcact ggtcaggaga
      301 actgattgta tcaagactcc aatcctgagg tgaccgatca ctattatagt gtctgacgga
      361 aaaaactata ctgatcattg tgggcatcaa agagtgggtg gttccgcagg ataaggtgtt
      421 ttttgatctt ttcgacagga tggcccagac ggtcgtctcc gcggccgata tgctcgttga
      481 gatcgtcgag aacttcgcaa agatcgagaa cgtggaagaa cagtgtcgtg cgatgaaaga
      541 gatcgaacac cagggagacg agatcaccca tgagatctac gagcagctga acctgacatt
      601 catcacaccg ctcgaacctg aagagatctc ccgccttgca tcagcgctcg atgacatact
      661 ggactacatc gacggcacca tgcaacagat gtacagctac ggtatcaccg agaccgacaa
      721 ctcaatggtt gaacttgcaa agctcgtcca gcttagcgtc atcgagatcg agaaggccgt
      781 aaacggcatc aggacgatca agaaccctac tcagatcgag gaacggtgca ttgaggtgaa
      841 ccgactggag aacatcgcgg acaacgtcct cggccgtgct atcatggacc tcttcaagac
      901 gaaagatgcg ataacaataa tcaagctcaa agacatctac gaaaaccttg agatagcgac
      961 cgataagtgt gaggacgccg caaatgtctt gagtgacata gccatcagac acacctgact
     1021 gctatggatc cgatcatcgt tcttggcatt atccttgcgt tactctttaa tttcgcaaat
     1081 ggtctgaacg acgcagcaaa ctcgatcgcc accatcatcg caacaaaagc cttaactccc
     1141 ctgcaggcgg tcctcctggc cggggtgttc aacctcctgg gccccctctt cttcaccact
     1201 gccattgcgg cgaccatagg caagggaata gtcgatccgg ccttcctcac gccgctgctg
     1261 atcctcatgg caatggtcgg cgcggtgctc tgggttctga taacatcttt ttttgggatc
     1321 ccggtatcga gcagtcatgc cctgatcggc gggctggtcg gggccgggat tgccgcagcc
     1381 gggactggag cggtcctctg gccaccggtt gcgctggttg ttcaaactgt cctttatatc
     1441 cttatcggtg tgtttctcgg tgcggcagtc gctgccgtag ccacataccg gagggatgag
     1501 tttaaattgc ggaacctcac cattggaggg ctcatcgggg tgacggtgat aatcccgctc
     1561 gccattgcaa ccggtttttt gaaaattggc ggccttcttg cggttatcat cttcattgtc
     1621 atctctccaa tgcttggttt tctctccgcg tttgtccttg ccacccttat aacctccatc
     1681 ttccgtaact atcccccaag gcgtctctcc agcctcttca agaacctcca gatcttttcc
     1741 ggatcgtttc aggcgatcgg ccacggcagc aacgatgctc aaaacgcgat gggcatcatc
     1801 acagccatgc tccttgcggg agggctgatc accgagtttc acgtcccgct ctgggtaatt
     1861 tttgcatcat cccttgccat atcacttgga acgctatttg gtgggtggcg ggtcattgac
     1921 aggatggcca acaagatcac gcggatccgg ccataccagg ggttctccgc atcgaccgct
     1981 gccggaggcg tcctttcact gatgaccgcc ttcggcgtac cggtctccac gactcatgtg
     2041 acaaccggtg caataatggg tgttggcaca acccgcggct actcagcggt caagtggggt
     2101 gttgtccgcg agatcctgat cgcgtggatc ctgaccatcc cggcatcggc gnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgaaccca agccgctttg agacccggcg
     2281 gacgtgtgta tcgacggcta taccaacgtt gataccgaac gcatgggaga ggacgatgtt
     2341 tgcggtcttc ctgccaacgc cgggcagcga cagtagttcc tccattgttt tggggacctc
     2401 gccgccgaac ccggagacaa tctttcttgc cgcgccaact atgtagcggg ctttcatgtg
     2461 atggaacccg atgctcctta tgagtggttc tacttcctcc ggttcggcgc tggcgagcgc
     2521 ctctggagtc gggtagcggg agaagagttc gtcgcgaatg gcgttgactg cgcggtcggt
     2581 agtctgcgcg gataggatag tcaggatcaa agcctcaaag gggttatgga actcgataaa
     2641 atgacgcctg ccgtcgacga tggggtagcg cgcaaggagg cggcggtaga cctcgcatgc
     2701 ggtcttgcgg ttcatgactg atggggcagg gcagatttga actgccgtca aagcgtccca
     2761 aacgctctag gatggaccag gctaccctac tgcccctgtt tacctgcata cattggggac
     2821 caagacaaaa atagttgccg ctccgtctct gtcggcacct gcacctgtat cgaccgctct
     2881 aacaatcgat cctcgaggag atgcacattc tctgatgtta accttccagg aaccgttgcc
     2941 ggaggggaaa aatggttata acctgacctt agaaatcggt catgatcctg aactggtcaa
     3001 cctcttcggt gttaagttct tccaggaact cttcagctgc atgtttgata ttcttgcttg
     3061 cggtgatggc cctgccaaca accaggatat cagcgccagc cctgagtgcc tctttcacaa
     3121 catgctgccg gatgccgccc gcggtcgcga tcagcatcct ctcaccgcct gccttccgga
     3181 ttgccggaat atcgccccag gcgtaatcgg tatcctcgat gtcgatagcc cggtggagct
     3241 caacaacatc cggcttcacc ttcagttgcc cgatgacggc aaccgggtca ctgacgttga
     3301 gcgtgtcaag cacgctgtag ataccggtct tccgggcttc ctggatggcc ttctcaatcg
     3361 tcgatagcgg ggcgaggccg gagatcacaa cggcgtcagc accggcgtca gcagccatgc
     3421 gggcctccag gttccccgta tccagcgtct tgaggtcggc gactatgaag gagcctggcc
     3481 tgatctcatg gatctcggag atgatcgaga gtccaaactt cttgataaga ggtgttccgg
     3541 cctcgaggat caggtggtcg ctctccggca gctcctcaag gaccttccgc atatggttcc
     3601 tgtcaacaag gtccatcgca acctgcaggt atggggggtt ccagagacgc tgaaccttga
     3661 atcccattac ggcatgggtt gcccgatctt tttcgtgaag gagcgtatcg acgtcaggga
     3721 aacgatcgag cgcccggcgg atcgcgagtt ttgtcgcgcc gtagttgaat cggtataact
     3781 tgttgtagtc ctctgcagtc gggtggacaa agacgcttgc catgatgacg atatcctcga
     3841 tatcgattcc ctcaaaggca ccctcctcca ccaggtcggc gacggccttt gccacagctg
     3901 actgcacagg cccgaagatc tggttcacct gatactcttt cttcagggtg accttcggga
     3961 tgatgatggt cgagggtttt gcaggcaggt ttggccttac taccgcaaga agcggggtat
     4021 ggcctttgga gagctgggag agggcgtttg caaacgcctg ccccacaggt ccttctttgt
     4081 ttcccatgat cagatctacg tgcgcaagtt ctgcgccgtc tccaaccagt gcttcgccga
     4141 tgagatacat atggatgccc tcagataact gctgaaatgt tggtggacct ggtttaaaga
     4201 cctcccggtt ggatcgtctc tcccctgggc agaaagcccc tgcagcgagg ggggctgatt
     4261 gctgtgccgg gtcagtttta tgcgcgcctc catcttcgga atgagtccag acgagcgcgc
     4321 gggttagacg aggtgggcct ggagaatgat tccatccatc tgatgattgc gcccgggata
     4381 actaccatca ttctggtgcg ttatggccca aacgagaaga gtgcaggctc gcctgcccgt
     4441 taagagttgc aagaatgtgg ggtcggtgag atttgaactc acgatcgacg ggtctctccg
     4501 aacatgcatc agtgctccaa cgggtcatca tccgtcaatc aggagaccca ttgttcatca
     4561 tgtgcaaaga ccgctggagc ccgtcgccat tccggactag gccacgaccc cattgtcctc
     4621 taatacattg atggcaatgc agtataatga ttgcgcctga aaaaaccagt aatcatataa
     4681 cgccgccgac acccatttat atagaatggt tgagggcagt tacgcgtgtg gaacctcgca
     4741 gatacctctg ctcggcatca ccatcggtga aatgctgaac cgtatagccg cggcgcaccc
     4801 ggacaacgag gcactcgtag ccgtccacca gggtatccgg tggacgtatg cggagtttct
     4861 cgatcgtgtc aacaccctgg cacgcgccct gatggcgctt gacgtggagc gcggcgatcg
     4921 ggttgccata tgggcgctga actacgcgga gtgggtgctt gttcagtttg ccacagcgaa
     4981 gatcggcgct atcatggtga atatcaaccc cgcatacagg acatacgagt tcgagtatgc
     5041 catgaagcag tctgaggtgc agacactctt catccagggc aggttcaaga cctccgacta
     5101 tgtcgggatg ttctatgagt cgtgccccga ggcttttgag gctaagccag gccggataga
     5161 cagcgacagg ttcccgttct tgaagaacgt catattcctt ggggatatcc catacaacgg
     5221 gatgtacacc tgggacgatc tcatgaagat ggccgacctt atcagccccg atgaactccg
     5281 ggagcgtgag gagtcgctcg acttcgacga cccggtcaac atccagtaca ccagcgggac
     5341 caccggtttt cctaaaggcg tggtcctgac ccatcacaac gtcctgaaca acggcttctt
     5401 cataggggaa ggtatggagt tcacccacca cgaccgactc tgcatccccg tgccgttcta
     5461 ccactgtttc ggtatggttc tctccaacat ggccagcgtc acacacggtg ccacgatggt
     5521 gctgccatcg ccagtctttg atgcggaggc tgtcctcaaa gccgtgcagg acgagcggtg
     5581 caccgccctc cacggcgtgc cgacgatgtt catcgccgaa cttgcgcacc cgaacttcga
     5641 gaactatcgg cttgactcgc tcaggacggg gatcatggca gggtcgccat gtccgacaga
     5701 ggtgatgcgg gaggtcaaca agaggatgca catctccgag atcgtgatcg tctacggcca
     5761 gaccgagacc tcaccgggga tcacgatgac cacgaccgat gatcccctgg aacggcgcgt
     5821 ctcgacggtt ggaagaccct ttcctcacac ggaggtcaag atcatcgacc cgaacacaaa
     5881 gaggatcgtc ccccgcggcg agaccggtga gatctgtgcc cgcgggtact gcgtgatgcg
     5941 gtgctactac aacaacccga acgccacccg ggcaactatc gacgagaacg gctggaacca
     6001 tacgggggat ctcggggtga tggacgagga gaactatgtc aagatagtcg gccgcctgaa
     6061 ggatatggtg atccgcggcg gtgagaacat ctacccgcgc gagatcgagg agttcctcca
     6121 cacccacccg aagatcgctg acgcctatgt gatcggggtg ccggacagga agtacggcga
     6181 ggaacttatg gcgtggatca gggtcgataa cggtgtgaga ctgacggagg aggatgtgcg
     6241 ggagttctgc cgcggcagga tcgcgcactt caagatcccg cgctacatca agttcgtcga
     6301 cgacttcccg atgacggt
//
LOCUS       DAOSZX010000083         6290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10500_length_6290_cov_28.963593, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000083 DAOSZX010000000
VERSION     DAOSZX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10500_length_6290_cov_28.963593"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1295)
                     /gene="mtrH"
                     /locus_tag="PK196_06785"
     CDS             complement(258..1295)
                     /gene="mtrH"
                     /locus_tag="PK196_06785"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HON41156.1"
                     /translation="MFKFEKEQVVHDFNGTKLGGQPGEYPTVLGASIFYNKHEIVLDD
                     KTGKIDKETAEALWNRCQELSDITGIPHFIQIIAEYGEAFESYIDWFCGIDDKTAFLM
                     DSSVPAALAHACEYVTQSGVADRAIYNSINGSISPENMEALAKSDVNAAIVLAFNPAD
                     PSVAGREKVLVEGGVAGQELGMLEIAEKCGITRPILDTAATPLGLGSGGAYREILACK
                     AIHGLPTGGAYHNMTVSWTWLKRWKGTKKNPSQQLAGLEGKDALVKQLLHHYLGGLDG
                     VRQAAWSAPDIGCNMIASTLGADLIMYGPIENVEAMITAQAYTDIVIMEAARDLGIEP
                     KAESHPFFKLV"
     gene            complement(1311..2048)
                     /gene="mtrA"
                     /locus_tag="PK196_06790"
     CDS             complement(1311..2048)
                     /gene="mtrA"
                     /locus_tag="PK196_06790"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HON41157.1"
                     /translation="MVEKKSPASGWPIVKGDFHAGDPQSCVAVVTMGSHLDEQAICDA
                     GAAIAGSCKTENLGLEKVIANVISNPNIRFVLCCGTEVKGHLAGQSFIALHANGVSGG
                     KIVGAQGAIPFIENLPEEAIKRFQEQVEIVNIMETEDLATIKAKIAELKARDPGAFPA
                     EPMIVEVKEAGAAAEVAVAGVNPQFLEIEERLDAIEEKIEFVDSEVAQRVGRKIGRDI
                     GILYGLVAGLIVFMMLLVLLPKLVEYL"
     gene            complement(2050..2262)
                     /locus_tag="PK196_06795"
     CDS             complement(2050..2262)
                     /locus_tag="PK196_06795"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit F"
                     /protein_id="HON41158.1"
                     /translation="MAEETTKATGPIRMTAINRMMESIRYKAQILARTNKLDSGIMDS
                     GIIGFAIGLALVMIFILVPALLLGGI"
     gene            complement(2268..>2527)
                     /locus_tag="PK196_06800"
     CDS             complement(2268..>2527)
                     /locus_tag="PK196_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HON41159.1"
                     /translation="GAFPAEPMIVEVKEAGAAAEEVTGEVQPLSGELALIHARMKVIE
                     RMVTDIGYRNRFAAGVYAGKIEGLMIGLIVSFVILGFILLG"
     assembly_gap    2528..2627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2628..3145)
                     /gene="mtrA"
                     /locus_tag="PK196_06805"
     CDS             complement(<2628..3145)
                     /gene="mtrA"
                     /locus_tag="PK196_06805"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HON41160.1"
                     /translation="MVEKKSPASGWPIVKGDFHAGDPQSCVAVVTMGSHLDEQAICDA
                     GAAIAGSCKTENLGLEKVIANVISNPNIRFVLCCGTEVKGHLAGQSFIALHANGVSGG
                     KIVGAQGAIPFIENLPEEAIKRFQEQVEIVNIMETEDLATIKAKIAELKARDPGAFPA
                     EPMIVEVKEAGAA"
     gene            complement(3157..3441)
                     /gene="mtrB"
                     /locus_tag="PK196_06810"
     CDS             complement(3157..3441)
                     /gene="mtrB"
                     /locus_tag="PK196_06810"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HON41161.1"
                     /translation="MAYVHVLPEYGLVVDPMIGVVTTVGVSYTPVLEQVAELEKITED
                     LVDMLSGEGSFLSSFPNREGVLKIAGGVTAFWYGMAIGLLIAGVVVLGLL"
     gene            complement(3444..4295)
                     /gene="mtrC"
                     /locus_tag="PK196_06815"
     CDS             complement(3444..4295)
                     /gene="mtrC"
                     /locus_tag="PK196_06815"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HON41162.1"
                     /translation="MTVKVEVGAGGVPHNKIMIYGLVGSLVCLYLTYLNTYLQAPYLA
                     FFGGLAAVAALVWGADTIKHLCSYGLGTGVPSAGMIALGSGVIAALFGATTGPLTPIV
                     TLIVAAIIGAIAGLLANHVVRMDIPAMVSSLTELAVIGAITVLGLVTMACGTFEFLGM
                     ITGTITFLGFTMQTYEMSVIGGGVIAAIFMLGAIAIQHAFNACLGPGEKQDRTLMLAA
                     ECGFLSMITVAIISFAFIATYAALLAFVISVIGWLYTYKQYIALSKRDAYAWLDAKPI
                     IEPKGGA"
     gene            complement(4296..5147)
                     /gene="mtrD"
                     /locus_tag="PK196_06820"
     CDS             complement(4296..5147)
                     /gene="mtrD"
                     /locus_tag="PK196_06820"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit D"
                     /protein_id="HON41163.1"
                     /translation="MTALGGPKQTAGVQPPTAMGIVLSIVLIVIALALAYLLLQMAGL
                     VALIGIIIGGALVAFGVHFVPVGGAPAAMGQSPGIATGVAMLAAGAGLAGLFAGAWAA
                     PLGLAIAVASGAIGGGLLMAITCTMVNIIYVFGMGIPAASGKVAKDPITGDTFPEYKS
                     QGTEGHGLPFVSWVGGVIGGALGGLGGTLIYLELLGVYQAQLPVILGATVEQIAPVAI
                     SLAGIFAVGMFLVNAVLAAYNITGTIEGPHDPKFKRFPRAIIASAVASAVAGIVALGL
                     FQLLGVF"
     gene            complement(5144..6043)
                     /gene="mtrE"
                     /locus_tag="PK196_06825"
     CDS             complement(5144..6043)
                     /gene="mtrE"
                     /locus_tag="PK196_06825"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit E"
                     /protein_id="HON41164.1"
                     /translation="MEEIIFGIGITALAGALATIAGAAEDTESDIGSQGDPNSQVQLA
                     PQMGYVHRIFNKAIAGEPPAYGLWVALGAGLAWAFMAMQINPILAIVLGSALAVFVQG
                     VYATTAYLGRTASLSKFEQPVYVDILKSMTTVTMAHAFVAIFATVTVCHLMNGALGHP
                     FPLPLLGLIWGIALGAAGSATGNPFYGKERQYQNQAFGAGVPIAASGNIVRYAEAGQR
                     NSIDNGFFSAKLGGPASGICFGLIVFFELWRTVVFEPVAAGWGAILVGVVVILIFAII
                     DRYIEVWARKTFGPYVAPEEASS"
BASE COUNT         1407 a         1851 c         1668 g         1264 t
ORIGIN      
        1 catactaaag cgtattcgct gaaaatacaa agatcttgca gttttggaat attctgagag
       61 gtgtagaggt taatgcggac gatcagatca cgccttgccc acccattgcc cggctattcc
      121 accagttcat ccaagaggag atcatatgaa gggctgtcgt gtttcatacg tgcaagcacc
      181 acactctctg cccgatggac tgtaccagcc acagactcaa ccgcaggaac taccctaccc
      241 agagaaaaaa agtggattta gactagtttg aagaatggat ggctctcagc cttgggctcg
      301 atccctaggt cacgcgctgc ttccatgatg acaatgtcag tgtaggcctg ggcggtgatc
      361 atcgcctcga cgttctcgat cgggccgtac atgatcaggt ccgcaccgag tgtgcttgcg
      421 atcatgttgc agcctatatc gggcgcagac caggccgcct gacgcactcc atccagtcca
      481 ccaagatagt ggtgcaggag ctgcttgacc agcgcatcct tgccctcgag gccggccagc
      541 tgctgcgagg ggttcttctt cgtacccttc cagcgcttca gccacgtcca ggaaacggtc
      601 atgttgtggt aggcaccacc ggtcggaaga ccgtggatcg ccttgcaggc aaggatctca
      661 cggtacgcgc cgcctgaccc aaggccgagc ggggttgctg cggtatcaag gatcgggcgg
      721 gtgatcccgc acttctctgc gatctcgagc atcccgagtt cctggccggc gacgccgccc
      781 tcgaccagaa ccttctcacg gccggcaacc gatggatcgg cagggttgaa ggcaaggaca
      841 atggctgcgt tcacatcact ctttgcaagc gcctccatgt tctccggcga gatcgaaccg
      901 ttgatggagt tgtagatcgc gcgatccgca acgccggact gtgtgacata ttcgcacgcg
      961 tgtgcaagcg ccgccgggac ggacgagtcc atcaggaatg cggtcttgtc atctatgcca
     1021 cagaaccagt cgatgtagct ctcaaacgcc tcaccgtact ccgcgatgat ctggatgaag
     1081 tgtggaatcc cggtgatgtc cgagagttcc tggcagcggt tccagagagc ctctgccgtc
     1141 tctttatcga tctttccggt tttgtcatcc agaacaatct cgtgcttgtt gtagaagatg
     1201 gatgcaccga gcacggtggg gtactctcca ggctgcccgc cgagcttggt accgttgaag
     1261 tcgtgtacca cctgttcttt ttcgaacttg aacatattcg tcaatcctcc ttacagatac
     1321 tcaaccaatt tgggcagtaa taccagcaac atcatgaaca caatcaaacc tgcgaccagt
     1381 ccgtaaagga taccgatgtc gcgcccgatc tttcttccga cgcgctgggc aacctccgaa
     1441 tccacgaact cgatcttctc ctcgatggcg tcgagcctct cctcgatctc gaggaactgc
     1501 gggtttacac ctgcaacagc cacttccgcc gcagcgcccg cctctttgac ctcgacgatc
     1561 atgggctctg cggggaaggc acccggatct ctcgccttaa gttctgcgat cttggccttg
     1621 atggttgcca ggtcctccgt ctccatgatg ttgacgatct caacctgttc ctggaaacgc
     1681 ttgatcgcct cttcaggaag gttctcgatg aacggaatgg caccctgagc accgacgatc
     1741 tttccgccgg agacaccatt cgcatgcaga gcaatgaaac tctgcccggc aagatgccct
     1801 ttcacctccg taccgcagca gaggacaaac ctgatgttgg gattggagat gacgtttgcg
     1861 atcaccttct caagaccaag gttctccgtc ttacaagacc cggcgattgc cgctccggca
     1921 tcgcagatgg cctgctcgtc gagatgcgaa cccatggtga cgacggcaac gcaactctgc
     1981 ggatcccccg catggaagtc gcccttgacg atcggccagc cactggccgg tgatttcttc
     2041 tcaaccatgt tagatccctc ccagcagcag tgcaggaacc aggataaaga tcatgacaag
     2101 ggcaagcccg atcgcaaacc cgattatccc cgagtccatt atgcctgaat ccagcttgtt
     2161 cgttctggca aggatctgtg ccttgtaccg gatggattcc atcatcctgt tgattgcagt
     2221 catccggatg gggccggttg cctttgtagt ttcttctgcc atttatatca ccccagcaag
     2281 atgaacccca ggatcacaaa cgagacgatc agacctatca tgagcccctc gatcttacct
     2341 gcatagacac ctgccgcaaa cctgttgcga tagcctatgt cggtgaccat ccgctcgata
     2401 accttcatcc gtgcgtgtat cagtgccagt tcgccagaaa gcggctggac ctcgccggtc
     2461 acttcctctg cagcagcgcc cgcctctttg acctcgacga tcatgggctc tgcggggaag
     2521 gcacccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca gcgcccgcct
     2641 ctttgacctc gacgatcatg ggctctgcgg ggaaggcacc cggatctctc gccttaagtt
     2701 ctgcgatctt ggccttgatg gttgccaggt cctccgtctc catgatgttg acgatctcaa
     2761 cctgttcctg gaaacgcttg atcgcctctt caggaaggtt ctcgatgaac ggaatggcac
     2821 cctgagcacc gacgatcttt ccgccggaga caccattcgc atgcagagca atgaaactct
     2881 gcccggcaag atgccctttc acctccgtac cgcagcagag gacaaacctg atgttgggat
     2941 tggagatgac gtttgcgatc accttctcaa gaccaaggtt ctccgtctta caagacccgg
     3001 cgattgccgc tccggcatcg cagatggcct gctcgtcgag atgcgaaccc atggtgacga
     3061 cggcaacgca actctgcgga tcccccgcat ggaagtcgcc cttgacgatc ggccagccac
     3121 tggccggtga tttcttctca accatgttct ttcacctcac agcagcccta atacaacgac
     3181 accggcaatc agaagaccaa ttgccatgcc ataccagaat gcggtcacgc ctccagcgat
     3241 cttgagaaca ccttccctgt tcgggaacga agacaggaaa ctgccctctc ccgagagcat
     3301 atctaccagg tcctcggtga tcttctcgag ttcagccacc tgctcgagca cgggggtgta
     3361 tgagacaccg actgtggtga cgacaccgat catcgggtcg acaaccagtc catactctgg
     3421 cagcacatga acgtaagcca ttctcaggca cctccctttg gttcgatgat aggcttggcg
     3481 tcaagccagg cgtatgcgtc acgtttcgag agggcgatgt attgcttgta ggtgtagagc
     3541 cacccgatga cagagatcac aaacgccagg agagcggcgt aggtcgcgat gaacgcaaac
     3601 gagattatcg cgacggttat catgctcagg aacccgcatt ccgcggcgag catgagtgtc
     3661 ctgtcctgct tctccccagg tccgaggcag gcgttgaacg cgtgctgaat ggcgatggcg
     3721 ccaagcatga agatcgcggc gatgacgccg ccgccgatga cagacatctc gtaggtctgc
     3781 atcgtgaacc cgaggaacgt gatcgtgccg gtgatcatac cgaggaactc aaacgtacca
     3841 caggccatcg tcacaagacc gagcacggtg atcgcgccga taaccgcgag ttcggtcagc
     3901 gaagatacca ttgccgggat atccatcctg acaacatgat tggccagcaa tccggcgatc
     3961 gctccgatga tcgcggcgac gatgagtgta acgatgggcg tcagtggccc ggtcgttgca
     4021 ccgaaaagcg cggcgattac acctgagccg agcgcgatca tacccgcgga cgggacaccg
     4081 gtacccagac cataactgca gagatgcttg atcgtgtcag caccccagac gagcgcagcg
     4141 acagcagcaa gcccgccgaa aaacgcgagg tacggcgctt gcaagtaggt gttgaggtac
     4201 gtcaggtaga gacagacgag cgaacccaca agaccgtaga tcatgatctt gttgtgaggg
     4261 acgccgcctg ccccgacttc aacctttact gtcatttaga atacccccag tagttggaac
     4321 agcccgagtg caacgatgcc tgcaacagcg gatgccacgg cggatgctat gatcgccctc
     4381 gggaaccgct tgaacttggg gtcatgcggc ccttcaatgg taccggtgat gttgtatgcc
     4441 gcaagcacag cgttgaccag gaacatgcct acagcgaaga tgcctgcaag cgagatcgca
     4501 acaggcgcga tctgctcgac cgttgcgccc agaattaccg gcagctgagc ctggtaaacc
     4561 cccagaagct caagatagat gagggtcccg ccaagaccgc caagcgcacc gccgatgaca
     4621 ccgccaaccc aggagacaaa cggcaggccg tgcccctccg tgccctggct cttgtactcg
     4681 gggaaggtgt cgcccgtgat tggatccttt gcgaccttgc ccgaagcagc cgggataccc
     4741 atcccaaaga cgtagatgat gttgaccatc gtacaggtga tcgccatcag aagcccgcca
     4801 ccgatcgcac cgctggcaac agcgatagca agtccgagcg gggcagccca tgcaccagca
     4861 aagagtccgg ccaggccggc gccggcagca agcatcgcga cacctgttgc gatgccgggt
     4921 gattgcccca ttgcagcagg cgcgccgccg accgggacga agtggacgcc gaacgcgacc
     4981 agggcgccgc cgatgatgat gccgataaga gctaccagcc cggccatctg cagcaggagg
     5041 taggcaagtg caagcgcaat gacgatgaga actatactga gaacgatacc catggctgtc
     5101 ggtggctgaa cacccgccgt ctgtttgggc ccaccaagtg cggtcatgac gatgcctcct
     5161 ctggcgccac gtatggaccg aaggtctttc ttgcccagac ctcgatgtag cggtcgatta
     5221 tggcaaagat gagaatcaca acaacgccga ccagtatcgc accccacccg gcagcgaccg
     5281 gttcaaatac cacagtgcgc cagagttcga agaagacgat cagcccaaaa cagatgcccg
     5341 atgccggacc gccgagtttg gcgctgaaga aaccgttgtc aatcgagttc cgctgaccgg
     5401 cctcggcgta gcggacaatg ttgccggacg cggcaatcgg aacaccagca ccgaaagcct
     5461 gattctggta ctgccgctcc tttccataga acgggttacc cgtcgcagat ccggccgcgc
     5521 caagcgcgat accccatata agacccagca gcggaagcgg gaaggggtga ccgagcgcac
     5581 cgttcatcag gtgacacact gttacagtag cgaagattgc tacgaatgcg tgcgccatgg
     5641 tcacggtcgt catcgatttc aagatatcga cgtagaccgg ctgttcgaac ttggagagac
     5701 ttgcagtacg cccaaggtat gctgttgtcg catagacgcc ctgcacgaag accgcgagag
     5761 cgcttccgag cacgattgca agtatcgggt ttatctgcat cgccatgaat gcccaggcaa
     5821 ggcctgcacc cagagcaacc cagaggccgt acgcgggagg ttcaccggca attgccttgt
     5881 taaatatgcg gtgaacgtat cccatctgcg gagccagctg aacctgcgag ttcgggtcac
     5941 cctgtgatcc tatatcagat tcagtgtctt ccgcagcgcc agctatggtt gcaagagcgc
     6001 ctgccaatgc ggtaatgccg atgccaaata tgatctcttc cattcagcat cctcctccgg
     6061 tgcccatgca ccgaagcata agttcaaaac ctttttggtt catttaacag ttgtttatga
     6121 tttaattaaa ggtttcgaaa agtttcgcca atatcgcgac aaaacgtaaa aattcgcccg
     6181 gatcgttctg attactccgt agtagttact ctaaagaaaa aaaagttgat ttagatttac
     6241 cgggccggta tgatgagcga acgctcgccg gcaggcatga actcgcggat
//
LOCUS       DAOSZX010000084         6247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10595_length_6247_cov_18.631621, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000084 DAOSZX010000000
VERSION     DAOSZX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10595_length_6247_cov_18.631621"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1553
                     /locus_tag="PK196_06830"
     CDS             45..1553
                     /locus_tag="PK196_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HON41165.1"
                     /translation="MEKSLDLINARIREGSARVVTAEEMPAIVDELGEEGALEEVDVV
                     TTGTFGAMCSSGAFFNFGHADPPIRMERVWLNDVEAYAGIAAVDAYLGATQEAETRDR
                     PYGGAHVLEEFVSGGTVELRATSHGTDCYPRRSITTELLLEDMNQAIMVNPRNAYQRY
                     NAATNTTDRTLHTYMGTLLPRCGNVSYSGAGTLSPIANDPGFRVIGSGVPIFLAGARG
                     MIVGEGTQHSAGTGFGTLMVTGDLKEMRQEFLRAAVMSGYGVTMYIGVGVPIPVLDTG
                     VVRSTAVRDEDIITDIIDYGTPRRDRPSLARVSYADLKSGSVEIGGETVRTSPLSSYR
                     RARAVARELKTWIEAGKMELALPTRRIDPAKRSKPMRETAVTPRVREIMDRRVISITE
                     DEDIRVAAKKLLKGETNHLPVVDASGLLVGIVTTYDVSKAVVSNGRFRQVKDIMTRNV
                     IRTTPDEPVDIAAQKLEQNNISALPVVDAGNRVVGILSAIDLGKLLPGRRWR"
     gene            1550..1948
                     /locus_tag="PK196_06835"
     CDS             1550..1948
                     /locus_tag="PK196_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41166.1"
                     /translation="MKLLLTFSRKGEHDPGREPVIARVVKETGVLINVEKANIDSMAG
                     EVLVDVPDGDADLIQRRFEEMGVSVRAVVDAIIRDEEECVDCGACISVCPQEVFSFDP
                     EWRLCVNAERCVLCGRCIQACPHGALSQQE"
     gene            1945..>2594
                     /locus_tag="PK196_06840"
     CDS             1945..>2594
                     /locus_tag="PK196_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HON41167.1"
                     /translation="MIRGHFEFRQTIATVLADDPGHIEAAKAGMLAARREVEDQIGRD
                     PFFAATFEPYIPPNAANTPTRMARAAAEAGVGPMAAVAGAIAGAGVEAMAAAGASFGL
                     IDNGGDIALVSDRQVTIGIYAGASPLSGRIAFRVPPQDEILGVCTSSATVGPSISFGI
                     ADAVTVFSPDVALADAWATAVCNQITAKDTSVLDRLEGSGVAGVLAIIGDTVIRWG"
     assembly_gap    2595..2694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2695..2962
                     /locus_tag="PK196_06845"
     CDS             <2695..2962
                     /locus_tag="PK196_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HON41168.1"
                     /translation="EDLDWAVYHGIPQTGSIAVEDLIAATGFEPDAVMASLERLEHYL
                     LIQRSGEMIRLLSIQESLIACRCRHSRVDLPFVIENGVIRAKKE"
     gene            2968..3492
                     /locus_tag="PK196_06850"
     CDS             2968..3492
                     /locus_tag="PK196_06850"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HON41169.1"
                     /translation="MPEAAVLRIGHRPERDQRVTTHVGLAARALGARGMYLAADDRGV
                     VASIQDVVSRWGGDFFVEDGVKWRQCIRDWKAGGGIVVHLTMYGLPMTDLVDEIRSLD
                     RVLIVVGAEKVPGEVYGLADYNVSVTTQPHSEISSLALFLDRLFEGKELNREYPDAKI
                     RIEPAKAGKKTVER"
     gene            3489..4577
                     /locus_tag="PK196_06855"
     CDS             3489..4577
                     /locus_tag="PK196_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HON41170.1"
                     /translation="MKPRVLVAGFATRHVAQSAYRAGYTVYAVDHYCDRDLSWYTRDS
                     LAFEELADLPDKIVELASRHPVDALVVTSGAETVGTAIPLYGTPPAKVERFLDKLEIQ
                     RFFEGLGVPVPPLADGRFPAMVKPRRGAGGWRNAVVMTEEELRRWEETWPDVPYIAQT
                     VVDGVPSSVSCIADGRCARAIAVNRQILRGEGERAHGFAGSITPFAHPLARKMIEAAE
                     RIAAASGCVGSVGIDFVAGDRPWAIEINPRFQATLDTVETAIGESVFEMHMNACRGVI
                     PAMRPPARQVAIRRILFAERDMRLDADLSGLAPRVADIPWPGTEFEEGQAILSVYGRG
                     GSEDEAYADLERSLALARKRSGEQGKGC"
     gene            4657..5178
                     /locus_tag="PK196_06860"
     CDS             4657..5178
                     /locus_tag="PK196_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HON41171.1"
                     /translation="MVSVAELLDDPAIKAYIRRMIGDEGIEVLRRFPVDGEHSDEELA
                     EITGISLNTVRHTLYTLYEKRLAEYRRLKNIETGWLTYLWHLQLDRIYDVIEEDLRDA
                     VEKLDARLSYEEKNDFYMCRDCSVIYTFADAADWNFACPNCDATLEHFDNELLVAALR
                     KRVEKIKESLGSA"
     gene            5171..5656
                     /locus_tag="PK196_06865"
     CDS             5171..5656
                     /locus_tag="PK196_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HON41172.1"
                     /translation="MREEDCIDLLRRAGCSAGVIAHCRAVRDLALTYASDPLIDRGLV
                     EAGALLHDIGRGVTHDIRHAEVGGEICRSFGLDEEVIAIVERHIGAGLTADECSLLNL
                     LPRDCMPRTAEEKIVAHADNLVKGTRVITLDERLLYAIALPERQRRRIYRLGVEVELF
                     R"
     gene            complement(5649..6152)
                     /locus_tag="PK196_06870"
     CDS             complement(5649..6152)
                     /locus_tag="PK196_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HON41173.1"
                     /translation="MWADISQEFADSPSQGRVVRFLLENGFGINEDGRIVCNGIEIPA
                     THIGRAIDTDRRVVDATARRILEKPELREIFTRMRAVPDLSGVAEALGLSVITLYPRD
                     AHQKGIVGAAVGVLVDHDLPIRQIYVTDPYLTEEPRLVMIVDEPRIPASVYERLRALP
                     QVKKLVI"
BASE COUNT         1193 a         1728 c         2053 g         1173 t
ORIGIN      
        1 gtaataaaca acacatataa atattccaga atggatatac gttcatggaa aagtccctcg
       61 atctcatcaa tgcccggata cgcgaaggta gtgcacgggt tgtcaccgcc gaagagatgc
      121 cggcgatagt cgacgaactg ggggaggagg gggcactgga ggaggtcgat gtcgtcacga
      181 ccgggacgtt cggggcgatg tgctcctccg gcgccttctt caacttcggg cacgccgacc
      241 cccccataag gatggagcgc gtctggctaa acgacgtgga agcatacgcc ggtatagcgg
      301 cggtcgacgc ctaccttggc gcaacccagg aggccgagac ccgcgaccgc ccctacggcg
      361 gtgcgcatgt cctggaggag tttgtctccg gtggcaccgt ggagttgcgg gcaacctctc
      421 acggtaccga ctgctacccc cgcaggagca taaccacgga gcttctcctc gaggacatga
      481 accaggcgat catggtgaac ccgcgaaacg cctaccagcg ctacaacgcg gcgacgaaca
      541 ccaccgaccg gacgctccac acctacatgg gcaccctcct cccgcggtgc ggcaacgtct
      601 cctactccgg tgcaggaacc ctctcaccaa tcgccaacga cccgggtttc agggtcatcg
      661 gaagcggcgt cccgatcttc cttgcaggtg cgagggggat gatcgtcggc gagggcacac
      721 agcactcggc gggcacaggg tttggaaccc tgatggtgac cggggactta aaagagatgc
      781 gccaggagtt cctgcgtgcc gcggtcatga gcggttacgg cgtgacgatg tatatcggtg
      841 tcggcgtgcc gataccagtt ctcgatacgg gggttgtccg gagcaccgcc gtccgcgatg
      901 aggacatcat tacggatatc atcgattacg gcacaccccg gcgcgaccgg ccgtcgcttg
      961 caagggtgag ttacgcagac ctgaagagcg ggtcggtcga gatcggtggt gagaccgtca
     1021 ggacatcacc tctatcgagt taccggcgtg cacgggcggt ggcccgtgaa ctcaagacct
     1081 ggatcgaggc cgggaagatg gaacttgcgc ttcccacccg caggatcgac ccggcgaaac
     1141 gctcaaaacc catgcgtgag acggccgtga ccccccgtgt ccgcgagatc atggaccggc
     1201 gggtgatcag catcaccgag gatgaggaca tcagggttgc agcaaaaaaa ctcctcaaag
     1261 gggagacaaa ccatctcccg gtcgtggacg cgagcggtct gctggtcggg atagtcacca
     1321 cctacgacgt ctcgaaggct gtggtgagca acggccggtt ccgtcaggtg aaggatatca
     1381 tgacgcggaa cgtgatcagg accacgccgg acgaacccgt ggatatagcc gcgcagaaac
     1441 ttgagcagaa caacatcagc gcgctgccgg tggtggatgc cgggaacagg gttgtcggca
     1501 tactgtctgc gatcgatctc ggtaaactgc tccccgggag gcggtggcga tgaaactcct
     1561 gctgacgttc tcccgcaagg gcgagcacga ccccggcagg gaaccggtta tcgcccgcgt
     1621 ggtgaaggag accggtgtcc tcatcaacgt cgagaaggcc aacatcgact cgatggccgg
     1681 cgaggtgctg gtggacgtcc cggacggcga tgctgacctc atccagcggc gttttgagga
     1741 gatgggggtg tctgtccggg cggtggttga tgccatcatc cgcgacgagg aggagtgtgt
     1801 cgactgcggt gcctgcataa gcgtatgccc gcaggaggtc ttctccttcg atcctgagtg
     1861 gcggctatgc gtgaacgctg aacgatgcgt cctctgcggc agatgcatcc aggcatgccc
     1921 gcacggcgct ctctcacagc aggaatgatc cgggggcatt ttgagtttcg ccagacgatc
     1981 gcgacggtcc tcgccgacga cccggggcat atcgaggcag cgaaggccgg gatgctggcc
     2041 gcccgacggg aggtcgagga tcagatcggc cgcgaccctt ttttcgcggc gacgttcgag
     2101 ccgtatatcc cccctaacgc cgcaaatacc ccgacgagga tggcccgcgc cgccgctgag
     2161 gcgggtgtcg ggccgatggc agcggtggca ggcgccatag ccggagccgg cgtggaggcg
     2221 atggccgcgg cgggggcgtc gtttggtctc atcgacaacg gcggggatat agccctggtg
     2281 agcgaccgcc aggtcacgat cgggatctat gcaggggcat cgcccttgag cgggcggatc
     2341 gcgtttcggg tcccgccgca ggacgagatc ctgggggtct gcacctcgtc ggcgacggtg
     2401 gggccgtcca tcagtttcgg tatcgccgat gccgtgacgg tcttttcccc ggacgttgct
     2461 ctcgcagacg cgtgggcaac ggcggtctgc aaccagatca cggcaaagga cacctccgtc
     2521 ctggacagac tggagggttc cggtgttgcc ggggtgcttg ccataatcgg ggatacggtg
     2581 atccgctggg gcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggagga
     2701 tctggattgg gccgtgtatc acgggattcc acaaaccggc agcatcgcgg tcgaagatct
     2761 tatagcagcc accggttttg aacccgatgc ggtcatggca tcgctcgagc gtctcgagca
     2821 ctacctcctg atacagcgct cgggtgagat gatacgtctt ctctccatcc aggaatcgct
     2881 gatcgcgtgt cggtgccgcc acagcagggt tgacctcccg tttgtcatcg agaacggtgt
     2941 aatccgggca aagaaggagt gaacgggatg cccgaggcag cagttctcag gataggccac
     3001 cgcccggaac gcgaccagcg ggtgacgacc cacgtagggc ttgcggcgcg ggcgcttgga
     3061 gcccggggga tgtacctcgc ggcggatgac cgcggggtgg ttgcgagcat acaggacgtg
     3121 gtctcccgct gggggggcga cttcttcgtg gaggacggtg taaagtggcg ccagtgcatc
     3181 cgggactgga aggccggcgg cggcattgtt gtccacctga cgatgtacgg gcttccaatg
     3241 accgatctcg tggacgagat ccgcagcctt gaccgggtgc ttatcgtggt cggtgctgag
     3301 aaggttccgg gcgaggtcta cgggcttgcc gactacaacg tctcggtgac cacacagcct
     3361 cactccgaga tctcgagtct tgctctgttc ctggaccgcc tcttcgaagg caaggaactc
     3421 aaccgggaat acccggacgc gaagatccgg attgaacctg cgaaagcggg taagaagacg
     3481 gtggaacggt gaagccgcgg gtgcttgttg ccgggtttgc aacacggcat gtggcgcagt
     3541 cggcatacag ggcggggtat accgtctacg ccgtcgacca ttactgtgac cgcgaccttt
     3601 cctggtacac gagagattcg cttgcgttcg aggaactcgc cgacctccct gataagatcg
     3661 ttgaacttgc atcccggcac ccggttgacg ctctggtggt cacctcgggt gccgagaccg
     3721 tcgggacggc gatacccctg tacggcaccc ctcctgcaaa ggtggagcgg ttccttgaca
     3781 aacttgagat ccagcggttc ttcgaggggc tgggtgtgcc cgttccaccg cttgccgatg
     3841 ggcggttccc ggcgatggtc aagccgcgcc ggggtgcggg gggctggagg aacgcggttg
     3901 tgatgacgga ggaggagctt cgccgctggg aggagacctg gccggacgtc ccctacatcg
     3961 cccagacggt tgtcgacggt gtcccttcaa gcgtctcctg catcgcggac ggccggtgtg
     4021 cgcgggcgat cgcggtcaac cgtcagatcc tgcggggtga gggagagagg gcgcacgggt
     4081 ttgccgggtc gatcactccg tttgcccatc cgcttgcgcg gaagatgata gaagcagcgg
     4141 agcggatcgc tgccgcaagc ggctgtgtcg gttcggtcgg gatcgatttt gtggccgggg
     4201 ataggccgtg ggcgatcgag atcaatccca ggttccaggc aacccttgat accgttgaga
     4261 cggcgatcgg agagagcgtc tttgagatgc acatgaacgc ctgccgcggg gttataccgg
     4321 cgatgcggcc tcctgcgcgg caggttgcaa tcaggcggat cctctttgcg gaacgggaca
     4381 tgaggcttga tgccgacctc tccgggcttg caccgcgcgt tgccgacatc ccctggcccg
     4441 gaaccgagtt tgaggagggt caggccatac tgagcgtcta cggccgcggt ggatcggagg
     4501 atgaggcgta tgccgacctt gagagaagcc tcgcgctcgc caggaaacga agcggggagc
     4561 aggggaaagg gtgttaaagg atggagaaga gataatgcag ggtatcgata ctttcgtgta
     4621 tgcacttata gggttaaacc gggagagatt ttgagtatgg tatctgtcgc tgaactgctg
     4681 gatgatcctg caatcaaggc ttacatcagg cgcatgatcg gggatgaggg gatcgaggtt
     4741 ctcaggaggt ttccggtgga tggggagcac agtgatgagg aacttgctga gattaccggg
     4801 atcagcctca acaccgtccg ccacaccctc tacaccctct acgagaaacg tcttgccgag
     4861 taccggcggc tgaagaacat cgagaccggc tggctaacct acctctggca tctccagctg
     4921 gaccgcattt acgatgtgat cgaggaagat ctccgtgatg cggtcgagaa actcgacgcc
     4981 agactctcct acgaggagaa gaacgacttt tacatgtgca gggactgcag cgtcatctac
     5041 acctttgcag acgcggcgga ctggaacttt gcatgcccga actgcgatgc cacgcttgaa
     5101 cacttcgaca acgaacttct tgtcgccgct ctcagaaaga gggtcgagaa gatcaaggag
     5161 agcctcggga gtgcgtgaag aggactgtat cgatctcctc cgaagggccg gttgttctgc
     5221 cggggtcatc gcccactgcc gggcggtgcg cgaccttgcg ctcacctacg cgtccgatcc
     5281 cctgatagat cgtggcctgg tcgaggccgg tgctctgctt cacgatatcg gccgcggggt
     5341 gacgcacgat atccgccatg ctgaagtggg tggcgagatc tgccgatcgt ttggacttga
     5401 tgaggaggtc atcgccatag tcgagcggca tataggtgcc gggttgacgg ccgatgaatg
     5461 ttcgttgttg aaccttcttc cgcgcgattg catgccccgg acggccgagg agaagatcgt
     5521 tgcccatgcg gacaacctgg tgaaggggac gcgtgttata acacttgatg agcggttgct
     5581 gtacgcgatt gcgcttcctg agaggcagag aaggcgtatc tacaggcttg gagttgaggt
     5641 agaactcttc agataaccag ttttttcacc tgcggaagcg cacgcagccg ttcgtagacc
     5701 gatgccggga tccgcggttc atccacgatc ataacgagtc ttggctcttc agtgaggtag
     5761 gggtcggtga cgtatatctg ccggatgggc agatcgtgat cgaccaggac gccgaccgct
     5821 gcgccgacaa tacctttctg gtgcgcgtct ctcgggtaga gcgttatgac agagagtccg
     5881 agcgcctcgg cgaccccgga gaggtcgggc acggcccgca tcctggtgaa gatctcccgg
     5941 agttccggtt tttcaaggat gcgccgggcg gtggcgtcga cgaccctgcg gtcagtatcg
     6001 atcgctcttc ctatgtgagt ggccgggatc tcgatgccgt tgcagacgat gcggccatct
     6061 tcgtttatgc caaacccgtt ctccagcagg aagcgaacca ccctgccctg cgacggtgaa
     6121 tcggcaaact cttgtgagat gtcagcccac atgggatgat cttggatcct ggtgcatata
     6181 tacacaccga tggcgatcat ggtgcgccgg ggtacatgtt cgttcaggcg tggttgcgtg
     6241 gtttatg
//
LOCUS       DAOSZX010000085         6145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10790_length_6145_cov_22.031527, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000085 DAOSZX010000000
VERSION     DAOSZX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10790_length_6145_cov_22.031527"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK196_06875"
     CDS             <1..585
                     /locus_tag="PK196_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HON41174.1"
                     /translation="PELCIGCRHCEIACAIEHSESRRLFDAIHEEKRSLPRIHVEVGV
                     NCLTFPNRCRHCDPAPCMQVCPTGSLCRDPVTGSVVVRYDRCIGCAVCAMACPFGIIH
                     FQRVHNLDTDREVNAKCDNCIERQKEGRIPACAEACKTGALEFGDANDLIRTSRRDFT
                     LRLTASEGTDAEVPKIPANVGAFRAILEEMANLG"
     gene            604..2493
                     /gene="cooS"
                     /locus_tag="PK196_06880"
     CDS             604..2493
                     /gene="cooS"
                     /locus_tag="PK196_06880"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HON41175.1"
                     /translation="MYDAFRSKRLAEQRALDAADQEIIAKALEDGVETAWDRFIDQQP
                     QCGFGQLGICCNRCAMGPCRIEPFGKKPDRGVCGATADTIVARNLLDDLSTGAAAHSD
                     HGREVLEVLLETAVGKSQGYQITDETKLKAIAAEYGINTENRKKEEIAEDVARAMLEE
                     YGTLKNRIQMAERAPEKTKDTWKRLGIVPRSVDREIVESMHRIHMGVGADYVNILLHA
                     MRTSLADGWGGSMMATEGSDILFGTPAPLRSRANLATLKQDMVNIVLHGHNPILSDMI
                     VKAADDPQLKERAAGKGAKGINIVGMCCTGNELLMRHGIPIAGTFLDQELAIATGAVE
                     VMVVDYQCIFPSIAQTAACYHTRVVSTSQKAKIPGTTYLEFRPETALDTAREIITVAI
                     DNFPNRNPGRVRIPQKPVDLMAGFSEEAIRKALGGTYKPLIDAIVGGRIRGAVGIVGC
                     NNPKIKQDYGHVTLAKELIKRDILVLETGCAAIACGKAGLLVPEAADLAGDGLGSVCK
                     ALGIPPVLHMGSCVDCSRILMLAANIAKELGVGIGDLPIGGAAPEWYSQKAISIGTYF
                     VSSGVYVVLGIPPKIFGSPNVTSLLAAQLTDLVNASFAVEPDPVKAADLLEAEIDRKR
                     KALGI"
     gene            2507..2992
                     /locus_tag="PK196_06885"
     CDS             2507..2992
                     /locus_tag="PK196_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HON41176.1"
                     /translation="MDERTLDAIIARHDAPAGKLLGILGDIQNQEGYIPREALEMLSD
                     KLGVRLSHLYSLATFYSYFSLKPVGDHVITVCMGTACHVKGAPRILEALEGMLGVECE
                     TAEDGRFSVTTDDNKFTLEIARCFGACSMAPVLRIDGNLYGYAAAEDLPAILAEYGWK
                     G"
     assembly_gap    3003..3102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3103..4533
                     /locus_tag="PK196_06890"
     CDS             <3103..4533
                     /locus_tag="PK196_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HON41177.1"
                     /translation="RVTADDAEEIVRAVEEGGVPEGKALCRIPSWDHVTGCTLTYGEG
                     FERIIPAYKDVPFFSHQKKVVLRNAGLINPEDIDEYIAVGGYAAAQKALTGMTPDAII
                     EEVEKSGLRGRGGAGFPTGLKWRITRDAKTDKKYIICNADEGDPGAYMNRNEMESDPH
                     MLIEGMIIGAYAMGAAEGLIYIRAEYPLAIRRLGIAIGQAREYGLLGEEVFGTGFSFD
                     IGIATGAGAFVCGESTALVASIEGRAGRPRPRPPRLTDIGGGLFGYPTDLNNVETWCN
                     VPVIISNGSDWYARIGAKGNSGTKVFSLVGKVDRVGLVEVPLGTSLNTIVFEIGNGGA
                     GGKKVKAVQTGGPSGGCIPVRLFDTPVDYESLNRVGSIMGSGGLVVMDEDTDMVDIAR
                     YFINFATTESCGKCVPCREGLKHTLLLLNKILAKKATRKDLETLADLSETIAATSLCG
                     LGQTAPNPVLTTLAYFREEYDALVGR"
     gene            4530..5336
                     /locus_tag="PK196_06895"
     CDS             4530..5336
                     /locus_tag="PK196_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HON41178.1"
                     /translation="MILEVTINGKRYRAEEGETILSVAIREKIEIPHLCYEASLDPYG
                     ACRLCMVEAVRRGRSEMTTACTLRAAEGLEIVTDTPQIVKHRKILFELYLAEAPRSEV
                     IKKMAARYGVTKTRFFKRVDPADPLGNRCVLCGLCVRACNEIMGAGAINFINRGPYTV
                     VNTPYFEPSADCLGCGACVNVCPTAAIDMEDIGGERVMRSWSGTRVPLAECESCGKHY
                     APKALAESIIARLETPLREETAKICPECRGKRIAREEILAKRGGGIEHAR"
     gene            5326..>6145
                     /locus_tag="PK196_06900"
     CDS             5326..>6145
                     /locus_tag="PK196_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON41179.1"
                     /translation="MPDDGARERKILDAAHKIQQNTRKATEKGFRIIISGKGGVGKTT
                     ITALLARIFAEKGFTTLAVDEDPQMNLPFAAGIPWDERGRIVPLTQNLDYVEEKTGAR
                     PGAGWGLMLTLNPDVSDVVERFGVRAPGGVNILVMGTVVQAASGCLCPENTLLESVIR
                     YINLREGEVILMDTPAGVEHFGRSIAKGFHQAVIVTDPSFNSVQVAERLARLAKELGI
                     PSVHLVINRVRSKDDTLKVREILGESMNLFSEQFFLPYEEDVIRCEPGVEPLLER"
BASE COUNT         1331 a         1797 c         1908 g         1009 t
ORIGIN      
        1 cctgaactct gtatcgggtg caggcactgc gagatcgcgt gcgcgatcga gcactcggag
       61 agcaggagat tattcgatgc aatccacgaa gagaagagat cgctgccgcg catccacgtg
      121 gaagtgggag tcaactgcct gacgtttccc aaccggtgca ggcactgcga ccctgcaccg
      181 tgcatgcagg tctgcccgac gggatcactc tgccgcgacc ccgtcacagg ttccgtcgtt
      241 gtccggtatg accggtgcat cgggtgtgcc gtctgtgcga tggcgtgccc gtttggcatc
      301 atccatttcc agagagtgca caacctcgac accgaccgcg aagtcaatgc aaaatgcgac
      361 aactgcatcg agcggcagaa agaaggcagg atcccggcct gtgccgaggc ctgcaagaca
      421 ggcgcactcg agtttgggga cgcaaacgac ctcatccgca caagcaggag ggatttcacc
      481 ctccggctga ccgcgtctga aggaaccgac gcggaagtcc cgaagatccc ggcgaatgta
      541 ggagcgttcc gtgcaatact ggaagagatg gcgaacctgg gatgaagaag gagtaagata
      601 ccaatgtatg atgcattcag atcaaagaga ctcgcagagc agagagcgct tgatgccgcc
      661 gaccaggaga tcatcgcaaa agccctggaa gacggcgtag agaccgcatg ggaccggttt
      721 atcgaccagc agccgcaatg cggcttcggg cagcttggca tctgctgcaa ccggtgtgcc
      781 atggggccct gccggatcga accgttcggc aagaaaccag accgcggcgt ctgcggggcc
      841 accgcggata ccatcgtggc gcggaacctg ctcgacgacc tctcaacagg ggctgcagcc
      901 cattcagacc atgggcgcga agtcctggaa gtcctcctcg agaccgcggt aggaaagagc
      961 cagggctacc agatcaccga cgagaccaaa ttaaaggcga ttgccgcaga atacggcatc
     1021 aacacagaga acaggaagaa ggaggagatc gcagaagacg ttgcccgtgc aatgctcgag
     1081 gaatacggga ctctcaagaa caggatccag atggcagaac gggctccgga gaagacaaaa
     1141 gatacatgga agagactggg catcgtcccc cgcagcgtcg atcgcgagat cgtggagtcc
     1201 atgcaccgga tccacatggg tgtcggcgca gactacgtca acatcctcct gcatgcgatg
     1261 agaacctcgc ttgccgacgg ctggggcggt tcgatgatgg caacggaagg ctccgatatc
     1321 ctctttggca caccggcgcc gcttcgcagc cgcgctaacc ttgccacgct caaacaggat
     1381 atggtcaaca tcgtcctcca cggccacaac cccatcctct ccgatatgat cgtgaaagcc
     1441 gcagacgacc cgcagttgaa agagcgtgcc gccgggaagg gtgcaaaggg catcaacatc
     1501 gtgggcatgt gctgcaccgg aaatgaactc ctcatgcggc acggcatccc gatcgcgggg
     1561 acgttcctcg accaggaact cgcgattgca accggtgcgg tcgaagtgat ggtcgtcgat
     1621 taccagtgca tattcccatc gatcgcacag accgccgcct gctaccacac cagggtcgtg
     1681 agcacgagcc agaaggccaa gatccccggg accacctacc tggagttcag gccggagacc
     1741 gcccttgata cagcgagaga gatcatcacc gtcgcaatcg acaacttccc gaaccgcaac
     1801 cccggccgtg tcaggatccc gcaaaaaccc gtcgacctta tggccgggtt ctcggaagag
     1861 gcaatcagaa aagcgctggg cggcacctac aagccgctga tcgatgcgat cgttggaggc
     1921 aggatcaggg gcgccgtggg catcgtcggc tgcaacaacc cgaagatcaa gcaggactac
     1981 ggccacgtga cgcttgcaaa agagttgatc aaacgggaca tactcgtcct cgagaccggg
     2041 tgcgccgcaa tcgcctgcgg aaaagcgggc ctgctcgtcc ccgaggctgc cgacctcgca
     2101 ggtgacggcc tcgggtccgt ctgcaaggcg ctcggcatcc cgcccgtcct gcacatgggt
     2161 tcctgcgtcg actgctcgcg catcctcatg ctggccgcaa acatcgcaaa ggaactcggc
     2221 gtcgggatag gcgacctccc catcggtggt gcggcacccg aatggtattc ccagaaagcc
     2281 atctccatcg gcacctactt cgtctcctcg ggcgtctacg tcgtcctcgg gatcccgccg
     2341 aagatcttcg ggagcccgaa cgtcaccagc ctcctcgccg cccaactcac ggatctcgtg
     2401 aacgcctcgt ttgccgttga gccagacccc gtcaaggccg cagacctgct cgaagccgag
     2461 atcgaccgga agagaaaggc gctcggcata taaacggaaa cccaccatgg acgaaagaac
     2521 gctcgatgca atcattgcgc ggcatgacgc ccctgccggg aaactcctcg ggatcctcgg
     2581 ggatatccag aaccaggagg gctacatccc gagggaggcg ctcgagatgc tctcggataa
     2641 actgggcgtc cgcctctccc acctctacag cctcgccacg ttctattcct acttcagctt
     2701 aaagccggtc ggcgaccatg tcatcacggt ctgcatggga accgcgtgcc acgtgaaggg
     2761 tgcgccccgg atcctggagg ccctcgaagg gatgctcggg gtagagtgcg agaccgcgga
     2821 ggatggcagg ttctccgtca ccaccgacga taacaaattc acgctggaga tagcccggtg
     2881 ttttggcgcc tgcagcatgg cgcccgtcct ccggattgac ggcaaccttt acgggtatgc
     2941 agcggcggaa gacctcccgg cgatccttgc cgaatacggg tggaagggat gaagatcaga
     3001 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagggtcac cgcggatgat
     3121 gcagaagaga tcgtccgtgc ggtcgaagag ggcggggtgc ccgaaggaaa ggccctatgc
     3181 cgcatcccgt catgggacca cgtgacgggg tgcaccctca cttacggcga ggggtttgag
     3241 cggatcatcc ccgcctacaa ggatgtcccg ttcttttcgc accagaagaa agtggtgctc
     3301 cggaacgccg gcctgatcaa ccccgaggat atcgacgagt atatcgcggt cggggggtat
     3361 gccgccgcac aaaaagctct gaccggcatg accccggatg ccatcataga agaggtggag
     3421 aagtccgggc tccgcggcag gggcggcgcc gggtttccca ccggcctgaa gtggcggatc
     3481 acaagggatg caaagaccga taagaagtac atcatctgca atgccgacga gggcgacccc
     3541 ggggcctaca tgaaccggaa cgagatggag agcgaccccc acatgctcat cgagggcatg
     3601 atcataggtg cgtatgccat gggggctgcc gaaggcctga tctatatccg cgccgagtac
     3661 ccgctcgcca tacgccgcct cgggatcgcc atcggccagg ccagggaata cggcctcctc
     3721 ggcgaggagg tcttcggcac gggtttctcg tttgatatag ggatcgcgac gggtgccggt
     3781 gcgttcgtct gcggtgaatc gacagcactt gtcgcctcca tcgaggggag ggccggccgg
     3841 cctcgccccc gcccgccgag gctgaccgat atcggcggcg ggctctttgg gtatccaaca
     3901 gacctcaaca acgtcgagac ctggtgcaac gtgcctgtga tcatcagcaa cgggagcgac
     3961 tggtatgcac ggatcggggc gaagggcaac tccggcacca aggtcttctc actcgtcggg
     4021 aaagttgacc gggtcggcct cgtagaggtg ccgctcggga cgtccctcaa cacaatagtg
     4081 tttgagatcg gcaacggcgg tgccgggggc aaaaaggtta aagccgtcca gacggggggg
     4141 ccgtcgggcg gctgcatccc ggtgaggctc tttgacaccc ccgtggatta cgagagcctg
     4201 aaccgggtcg gctcgatcat gggttcgggg ggcctggtcg tcatggacga ggatacggac
     4261 atggtggaca tcgcccgcta cttcatcaac ttcgcgacga ccgagtcctg cgggaaatgc
     4321 gtcccctgcc gggaggggct caagcacacg ctcctcctcc tcaataagat cctggcaaag
     4381 aaagcgacaa gaaaagacct cgagaccctt gccgacctct ccgagaccat cgcggcgacg
     4441 tcgctctgcg ggctcggcca gacagcgcca aaccccgtgc tgacgaccct ggcttatttc
     4501 cgggaagaat acgatgcgct ggtgggacga tgattcttga ggtcaccatc aacgggaaga
     4561 gatacagggc ggaggagggg gagaccatcc tctcggttgc gatacgggag aagatcgaga
     4621 tcccgcacct ctgctacgag gcttcgcttg acccctacgg cgcctgccgg ctctgcatgg
     4681 tcgaggccgt caggaggggg aggagcgaga tgaccacggc ctgcacgctg cgggcagccg
     4741 aagggctgga gatcgtgacc gataccccac agatcgtaaa gcaccgcaag atcctctttg
     4801 agctctacct tgcagaggcg cccagatcag aggtcatcaa aaagatggca gcccgctacg
     4861 gggtgacaaa gacacggttt ttcaagaggg tcgatcccgc cgacccgctc gggaacaggt
     4921 gcgtcctttg cgggctctgc gtccgggcat gcaacgagat catgggggcg ggtgcgatca
     4981 acttcatcaa ccgcggccct tacacggtgg tcaatacgcc gtatttcgag ccgagcgcgg
     5041 actgtctcgg ctgcggtgcg tgcgtgaacg tctgccccac cgctgcgatc gatatggaag
     5101 atatcggtgg cgagcgggtc atgagatcct ggagcggcac aagagtcccg ctggcggagt
     5161 gtgaatcctg cgggaagcat tacgcgccga aagcgctcgc ggagtccatc atcgcccggc
     5221 tggagactcc cttaagagag gagacagcaa agatctgccc ggagtgccgg ggaaaacgga
     5281 tcgcacgcga agagatcctt gcaaagcgcg ggggtggaat agagcatgcc cgatgacggg
     5341 gccagggaga gaaagattct tgatgccgcc cataaaatcc agcaaaacac acggaaagcg
     5401 accgagaaag ggtttcggat catcatcagc gggaaagggg gtgtcgggaa gaccacgatc
     5461 accgccctcc tggcccgcat ttttgcagag aagggattca ccacccttgc cgttgatgaa
     5521 gacccccaga tgaacctccc gttcgcagca gggataccct gggacgagag gggccggatc
     5581 gtcccgctga cacaaaacct ggattacgtg gaggaaaaaa ccggtgcacg ccccggcgct
     5641 ggctggggtc tcatgctgac gctgaacccc gacgtctccg acgtggttga gcggttcggg
     5701 gtgagagcgc ccggcggggt gaacatactt gttatgggga cggtggtgca ggcggcaagc
     5761 ggctgcctct gcccggagaa caccctcctt gagtccgtca taaggtatat caacctccgc
     5821 gagggggagg tcatcctcat ggacacccct gccggggtgg agcatttcgg gcggtcgatt
     5881 gcaaagggtt ttcaccaggc ggtgattgtc accgacccga gcttcaactc ggtgcaggtg
     5941 gcggagcgcc tggcgcgtct ggcaaaagaa ctgggcattc cttcggttca tctcgtgatc
     6001 aaccgggtgc gttcgaaaga tgatacactg aaggtccggg agatcctggg cgagagcatg
     6061 aacctctttt cggagcagtt cttcctcccc tacgaggagg acgtgatccg gtgcgaaccc
     6121 ggtgttgagc cgctgctgga gagag
//
LOCUS       DAOSZX010000086         6073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_10966_length_6073_cov_21.451147, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000086 DAOSZX010000000
VERSION     DAOSZX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10966_length_6073_cov_21.451147"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            578..1630
                     /locus_tag="PK196_06905"
     CDS             578..1630
                     /locus_tag="PK196_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HON41180.1"
                     /translation="MCGIFGVMDKRRQLMDGSGIQQALSMMNERGSGEGAGYAAYGIY
                     PDYQNCYALHVFFDNAPESKAVVDATLERWGTVEHDEAIPTYNQPNLRATSTPWRYFF
                     RPDTSLAPDSASPEKDLITSLVMQINTGSNGARVISSGKNVGVFKAVGWPEDVANFYR
                     IEDYEGYIWLAHNRYATKDPGKWERPDPFNLHDWSVAEDGKITSYGTNRRYIESFGYA
                     CSMSTDSEIITYLIDILVRRHGLDINLAIRALAPPFWEDIDRMPKPEGDLNRALRLAY
                     GSAMMNGPFTVVMANPDMMVGFTDRGKLRPMVVGECGDRLYISSEEAAIRAMEPNVES
                     ITMPAAGEPVIGRVAP"
     gene            1627..3144
                     /locus_tag="PK196_06910"
     CDS             1627..3144
                     /locus_tag="PK196_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HON41181.1"
                     /translation="MSSNLGSMPLRYRVTIDRERCMECERCIENCSYGVFWKDGNRIM
                     INSRKCTACHRCLAYCPRDAISIEEHPCDYRSHPVWTRQIREAIYNQARTGKIILAAM
                     GSVADLPVIFDHLMLDASQVTTPPTDPLREPVELRTYLGKKPSKLDIRRNSRGDIELA
                     TDLAPNLMLETPIMLGHMSYGAISLNAQISMARAAKEMGTYMGTGEGGLHAGLYPYQD
                     RMIVQVASGRFGVNIDYLERGAAIEIKLGQGAKPGIGGHLTGEKVSKDVSRTRMIPEG
                     SDAISPAPHHDIYGIEDLSQLVRAIKEATEWKKPVFVKIAAVNNTAENVAAVARSSAD
                     AIVIDGFRGGTGAAPRVFRDHVGIPIEVAIASADRELCNQGLRNEVSLIACGSIRESA
                     DAIKAIALGADAVYIGTAALAAMGCRVCGNCYQGLCPWGIATQRQDLVQRLDPDVASK
                     QVANLIHAWTMEITELMGAAGINSIESLRGNRERLRGYMLDENLLNVLDVKPIGA"
     gene            3151..3888
                     /locus_tag="PK196_06915"
     CDS             3151..3888
                     /locus_tag="PK196_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41182.1"
                     /translation="MRQLVTIDAKGMHYTPLNREIRKAVEDGAQEVVVKNVLGQRFIG
                     DGLRGNATIHIYGVPGGDLAMFMSGPTIIVHGNADHAPGNTMDAGKVVIHGSAGDAVA
                     HSMRGGKVFVRGDIGYRGGIHMKQYETQRPVLVVGGAARTFLGEYMAGGLLIVLDLNG
                     TMKAREIGSGIHGGEIIIRGDVDDACLAAGAKTVPLTDEDRERIVPVIREFSGDFGLD
                     PEPLINADYTRIVPASARPFAGKYTWE"
     gene            3894..4913
                     /locus_tag="PK196_06920"
     CDS             3894..4913
                     /locus_tag="PK196_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HON41183.1"
                     /translation="MTTKTFKDLEAEVWAMDLCSGCGACVAVCPADALRFAPGNTDAP
                     VNTGYCKSENDSVPCGACYAACPRVDLASQGRMLGRYQDIVAARSVFPVERKQSGGAV
                     TAILVNALDEGLIDAVVTVTSDPWTMKPSSAVITNSEALVQHAGSRYSWWVPLLASLK
                     EAVVTRKYRRIAVVGVPCVTRATKAIRESDHELLRPYAKAIRIVIGLFCTETFDYAKL
                     VEGKLQSERGIDPWEIRRMDIKGKLDVYLQDERRVSIPLAELQGAVRPGCLVCTDFTA
                     VEADISAGAVGSPEGYTTLVIRNDIGRGFVNRAIWQGKLATGESVDIAAIERLAAKKA
                     ERLTK"
     gene            4970..5116
                     /locus_tag="PK196_06925"
     CDS             4970..5116
                     /locus_tag="PK196_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41184.1"
                     /translation="MQHQYYEIGAAISGVTRDAPDDREAEAVSGIPYVMNAYRDAAEA
                     ILDS"
     gene            5222..5971
                     /locus_tag="PK196_06930"
     CDS             5222..5971
                     /locus_tag="PK196_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41185.1"
                     /translation="MRSINIRTWEVRLGIALVLSSIIVYTVKYLLLGDPENTYHYIFN
                     ALGFLPINVLLVTLILNHLLSVREKRKRLEKLNMVIGTFFSEVGTGLLAFLSDRDPAL
                     PEIRHDLIVTDAWTPEKFSTVRERSRRRHSRLNIDAADLQELCRYLTEKRGFLLQLLE
                     NPVLLEHESFTELLRAVFHLTEELERRGDFAALPASDVGHLAGDVDRVYDRLIGEWLA
                     YMEYLQRNYPYLFSLAMRSNPFDEAASPIVR"
BASE COUNT         1380 a         1730 c         1823 g         1140 t
ORIGIN      
        1 agagcctgtg catcctgcgc tgaatgagaa gacgagcagg agcgcaacca gtaggggtag
       61 caacctgaag ttcatatgcc atgagtgggt atcaatgtat ttcacctttt cgccgcgccc
      121 cgctatgggc cgatagccat gcaagttacc tgatcttaca accgccagac accgggtatc
      181 tccgaaaaac tacatgatgc ccacaaggcc acaggcatac cagatgaaaa tatctagagg
      241 ggccgttttc atacgaaaca aatgagcaac acagtccctc cgttcatggg attatgacag
      301 tcgattggaa aaataaaccg caccgccgtg tagtccggca cccccaggga gcagcatatc
      361 aacctgccca accaggataa ctacgtcttt tggcgtaccc acaatgtcgg cgaactggag
      421 gcgtggggat cagacactat cctggtgaat acggtaaagc cgtcatcaga cacatggaag
      481 tctgcttcca tcagagggta taaatatcta ttgttacaaa cctgaacaag gaaaaatata
      541 ggatacagca gtagaacact gatctaccga ggttacaatg tgcggcatat ttggtgtaat
      601 ggataagagg cgccagttga tggatggatc tggcatccaa caggccctct ccatgatgaa
      661 cgagcgcggt agtggtgagg gggcgggcta cgcggcatac ggcatatacc ccgactacca
      721 gaactgctac gcgcttcacg tcttcttcga caacgccccc gagagcaagg cagtcgtcga
      781 tgcaacactg gaacggtggg ggacggtcga gcacgacgaa gcgatcccca cctacaacca
      841 gccaaacctc cgggccacca gcaccccctg gcgttacttt ttcaggcctg acacctccct
      901 cgcgcccgac agcgcatcac cggagaagga tctcatcaca agccttgtta tgcagataaa
      961 cactggctca aacggggcgc gggttatctc atccgggaag aacgtcggtg tcttcaaggc
     1021 agtcggctgg ccggaagacg tggctaactt ctaccggata gaggactatg agggctacat
     1081 ctggcttgca cacaaccgat acgcaactaa agacccggga aagtgggaac ggcccgaccc
     1141 gttcaacctc cacgattgga gcgttgcgga ggacggaaag atcacttcct acgggaccaa
     1201 ccgacgctac atcgagagtt tcggttacgc ctgctccatg tccaccgaca gcgagatcat
     1261 cacatactta attgacatcc tggtgaggcg gcacggcctt gacatcaacc tcgccatcag
     1321 ggcgcttgcc ccgccattct gggaggacat cgaccggatg ccaaaacctg aaggagatct
     1381 gaatcgcgcc ctccggctcg cctacgggtc agcgatgatg aacggaccgt tcaccgtcgt
     1441 gatggcgaat cctgatatga tggtcgggtt taccgaccgg ggcaaactcc ggccgatggt
     1501 tgtcggtgaa tgcggagatc gtctctatat atcgagcgaa gaggcagcga tccgggctat
     1561 ggagccgaat gtcgagtcga tcaccatgcc ggctgcagga gagccggtga tcgggagggt
     1621 tgcaccatga gcagtaacct cggcagcatg ccacttcgtt accgggtcac aatcgatcgg
     1681 gagcggtgta tggaatgcga gcggtgcatt gagaactgct cctacggtgt attctggaag
     1741 gatggcaacc ggatcatgat caactcccgg aagtgtaccg cctgtcaccg gtgtcttgct
     1801 tattgcccca gggacgcaat cagcatcgag gagcatccct gcgactaccg gagtcacccg
     1861 gtctggaccc ggcagatcag ggaggcgatc tataatcagg cccggacagg caagataatc
     1921 cttgctgcaa tggggagcgt cgccgatctt ccggtcatct ttgatcatct gatgctggac
     1981 gcaagccagg tcaccacacc cccaaccgat ccgctccgcg aacccgtcga actccggacg
     2041 tacctcggga agaagccgtc aaaactggat atccggagaa attcaagagg ggatatcgaa
     2101 ctcgccacag acctcgcccc gaacctcatg ctcgagaccc cgatcatgct tggccacatg
     2161 agttatggag cgatcagcct caacgcccag atctccatgg ccagggcagc aaaggagatg
     2221 ggcacctata tgggtaccgg ggagggtggg ctccatgccg ggctctaccc ctaccaggat
     2281 cgtatgatcg tccaggtggc atcgggccga ttcggcgtga acatcgatta cctggaacgc
     2341 ggggctgcca tcgagatcaa actcggtcag ggcgcgaaac ctggcatcgg cgggcacctc
     2401 actggcgaaa aggtcagcaa agacgtctcc cgtacccgga tgatcccgga aggaagcgat
     2461 gccatcagtc cagcaccgca ccatgatatc tacggcatag aagacctctc acagctcgtc
     2521 agggccatca aggaagcaac ggagtggaag aaaccggtat ttgtcaagat cgcggccgtc
     2581 aacaacacag ccgagaatgt ggctgctgtt gcccgatcat cggcagatgc catagtcatc
     2641 gacggattcc ggggcggtac aggcgcagca ccgcgggtct tccgcgacca cgtcggcata
     2701 ccgatcgagg tcgcgatcgc gagcgctgac cgcgaacttt gcaaccaggg actcaggaac
     2761 gaggtctccc tcatcgcctg cggcagcatc agggagagcg cagatgccat aaaggcaatc
     2821 gccctcggcg cggacgcggt ctatatcggc accgcggcgc tcgctgcaat gggatgccgg
     2881 gtctgcggga actgttacca gggtctctgc ccatggggga tcgccaccca gcgtcaggac
     2941 ctggtgcagc gtctggaccc cgatgtggca tcaaagcagg tggcaaacct gatccatgca
     3001 tggacaatgg agatcaccga actcatgggc gctgcgggga taaacagcat cgagagcctg
     3061 cggggcaacc gcgagcggct caggggctat atgcttgatg agaacctgct caatgttctt
     3121 gatgttaaac cgataggggc gtgaacgatc atgagacaat tggtcacaat cgatgcgaag
     3181 gggatgcact acacgcccct gaaccgggag atacggaagg ctgttgagga cggtgcccag
     3241 gaggttgtcg tcaagaacgt cctcggacag cggttcatcg gcgatggtct ccgggggaat
     3301 gccaccatcc atatctacgg tgtcccgggc ggtgacctcg cgatgtttat gagcgggccg
     3361 acgatcatcg tccacggaaa cgctgaccat gcgcccggca acacgatgga tgccggaaag
     3421 gtggtgatcc acggaagcgc aggtgatgcg gtggcgcaca gcatgcgggg cggcaaggtc
     3481 tttgtccggg gtgatatcgg ctaccgcggc gggatccaca tgaaacagta cgagacccag
     3541 cggccagtcc tggttgtcgg gggggccgcc cggacgttcc ttggtgaata tatggccggt
     3601 ggcctcctga tcgtcctcga tctcaacggg accatgaagg cacgggagat cgggagcggg
     3661 atccacggcg gtgagatcat catccgcggc gatgtagacg atgcctgcct cgcggcaggg
     3721 gcaaagacgg tgccgctcac cgacgaggac cgggaaagga ttgtcccggt gatcagagag
     3781 ttttcaggtg acttcgggct cgatccagaa ccgctgatca acgctgacta cacccggatc
     3841 gttcccgcga gcgcaaggcc ctttgcaggg aagtatacgt gggagtgatt gggatgacta
     3901 caaaaacttt caaggatctg gaggctgagg tctgggccat ggatctctgc tccggatgcg
     3961 gggcgtgcgt ggcagtctgc ccggcagacg ccctcaggtt cgcaccgggg aacactgatg
     4021 ctccggtgaa cactggctac tgtaaatccg agaacgacag cgtgccctgc ggggcgtgct
     4081 acgcggcctg cccgagggtt gacctggcgt cacaggggag gatgctcgga cggtatcagg
     4141 atatcgttgc ggcccggtcg gtattcccgg ttgagcggaa gcagagtggt ggcgcggtga
     4201 cggcaatcct cgtcaacgct ctcgatgagg ggctgatcga cgcggtggtc acggtaacga
     4261 gcgacccctg gacgatgaaa ccttcgtctg cggtgatcac caactcggag gcgcttgtcc
     4321 agcatgccgg aagccgctac tcctggtggg tgccgctcct cgcctccctc aaggaggcgg
     4381 tcgtcacccg gaagtaccgg cggatcgccg tcgttggcgt gccctgcgtg acgcgggcga
     4441 caaaggcgat ccgggagagc gaccacgaac tcctcagacc ctacgcaaag gcgatccgga
     4501 tcgtgatcgg cctgttctgc acggaaacct tcgattacgc gaaactggtc gagggtaaac
     4561 tgcagtcaga gcgcgggatc gatccatggg agatccgccg catggacatc aaagggaaac
     4621 tggacgtcta cctccaggac gagcggcggg tatccatccc actcgctgaa ctgcaggggg
     4681 cggtcagacc gggatgcctg gtctgcaccg actttacggc agtggaggca gatatatcgg
     4741 ccggagcggt tggatcacct gaagggtata caacgctggt catcaggaac gatataggta
     4801 ggggtttcgt caaccgggcg atctggcagg gaaaacttgc gaccggcgag agcgtcgaca
     4861 tagcggccat cgaacgcttg gcagcgaaga aggcagaacg gctgactaaa taattttttt
     4921 tacgaaataa gcaggcagaa ggccccccac tgatactacc gtgaccagaa tgcagcatca
     4981 atattatgag attggggctg caatctcagg ggtaacccgg gatgcccccg acgataggga
     5041 ggcggaagcg gtctctggta tcccctacgt gatgaacgcc tatcgcgatg ctgccgaagc
     5101 aatcctggat tcgtgatcgg gagacctcca ccctgccggc ggtgaactct gccctacccc
     5161 cttgtgaggc attgaggggg ccggccaaca ctttttctgc gcaaaagatc gatggcataa
     5221 tatgcgcagc ataaacatcc ggacctggga ggtgcggctg ggtattgctc ttgttctttc
     5281 atccatcatc gtctataccg tgaagtacct cctgctcggc gacccggaga acacctacca
     5341 ctacatcttc aacgccctgg ggtttctgcc gataaacgtt ctccttgtca ccctgatcct
     5401 gaaccacctc ctgagcgtca gggagaagcg caagcggctg gaaaaactga acatggtcat
     5461 cggcaccttc ttctcggagg tgggtaccgg cctcctggcg ttcctttctg accgcgatcc
     5521 ggcccttccg gagatccggc atgacctcat cgtgacagac gcctggacac cggagaagtt
     5581 ctccacagtt cgcgaacgta gccggaggcg tcactccagg ctcaacatag atgcagcaga
     5641 cctgcaggaa ctctgccgct acctgacaga gaaacgcggt tttctcctcc aactgctgga
     5701 gaaccccgtg ctgcttgagc acgagtcgtt cacagaactc ctccgggcgg tctttcacct
     5761 taccgaagaa cttgagcgcc ggggggactt tgccgctctc ccggcaagcg acgtggggca
     5821 cctcgccggg gatgtggacc gggtttacga tcgcctgatc ggggaatggc tcgcttacat
     5881 ggagtacctc cagcgcaact atccctatct cttctcactt gcgatgcgga gcaacccctt
     5941 cgatgaggcg gcgtccccga tcgtgaggtg aagacatgag gttgagagcc gcaaacaaca
     6001 gaggctctct ctcacgcttt tttttgcggt ggtgtacggc ctgcggtatt tttgccaccc
     6061 cgtctcagac acc
//
LOCUS       DAOSZX010000087         6056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11008_length_6056_cov_21.955507, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000087 DAOSZX010000000
VERSION     DAOSZX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11008_length_6056_cov_21.955507"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..757)
                     /gene="pyrF"
                     /locus_tag="PK196_06935"
     CDS             complement(122..757)
                     /gene="pyrF"
                     /locus_tag="PK196_06935"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HON41186.1"
                     /translation="MTELILALDVSDRKEALNIAESCAPFIDAIKVGYPLVLSTGLSI
                     TEDLAGPGLPLIADFKVADIPNTNRLICEAVFSAGFDAVIAHGFVGADAARACIEVAH
                     SHSGAAYIVAEMSHPGATEFFHDGVAERIAALAVRCRADGIIAPATRPERVTRLREIV
                     GDKAIYSPGIGAQGGDLDAVARLVDGVIVGRSIYAADDPAAAAERLSRIRR"
     gene            complement(754..1689)
                     /locus_tag="PK196_06940"
     CDS             complement(754..1689)
                     /locus_tag="PK196_06940"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HON41187.1"
                     /translation="MTSRYGDAVEQARVRPGMTVGELVDELGKAGAYNGGSLWQAVNI
                     YEQMLRDDKALKFFGLSGAMVPGGMGGIVADLIRRRHIDLLVSTGANLTHDVIEAIGC
                     HHYHGNAQVSDTELYEEGINRIYDIFLPNEAFIRFEEFLQDVYSSLPEGATISIADLL
                     NLIGSRLKTGILAEAAMAGVPVYCPAIQDSMIGLQYWLFSQTHNVTVSAFADMSGMLD
                     RCFEAQRAGAILVGGGVPKNYILQSKLMTETGFDYAVQLTGDRPDLGGLSGATLEEAR
                     SWGKLTGEATAVTVYGDATITLPLLVAATLERLER"
     gene            complement(2126..3433)
                     /locus_tag="PK196_06945"
     CDS             complement(2126..3433)
                     /locus_tag="PK196_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HON41188.1"
                     /translation="MWIGIDDTDSPAGMCTTYIGAVLVRQLEHAGIRVADTRLVRLNP
                     NVIHKTRGNAAVCIEAVGDLEEAFRLATVCVEELAEFGDPQTNPGVAVSPVRPPPDFY
                     YAALRDFCTVDEAVRVLESAGALYRGYKNRRGLIGATAAIASEFPDKTYELLAYRKRE
                     AWGTPRRVDAGSLFHAEEMTYPHTWDTVDRENGMVVCVPHTPDPVLFGIRGESPAWVD
                     MARLFVRSEATACEQVYATNQGTDAHLIAGSIGTLREGRSYLVGGRVTGSPKTGRGGH
                     VSIILEDDGAEVRCMAYEPTKGFRDVVRVLLPGDIVMAAGSYKGGSLNLEKIGVVRAA
                     NAVRVRPPRCRACGKRMTSAGRGKGYKCRTCVERSHNPEVEQIERHIRPGWYEVPPSA
                     RRHLARPLARGLPLWEENLFQSIRKGGIPSLQVNQMIFTKKDL"
     gene            3482..4396
                     /locus_tag="PK196_06950"
     CDS             3482..4396
                     /locus_tag="PK196_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HON41189.1"
                     /translation="MSQDRLSQMVISIMLLAGFDVSERCNIRPRSFDLIAKKGDTLVT
                     IKVVSHIDSITADTAWDLNLIARCLEATPLIIGERARDTDLERGVVYIRYGLFAISPE
                     TLYDYLVEGNSPMVYASPGGLYVKIKGDLLREMRERSGMSLGDLASHLGVSRRTISKY
                     ESGMGTTLEIAIRLEEVFNAPLVETIDLIGYHSPEPVKPPEPVSGDILSDLERIGMEI
                     YAMRQAPFQALALFNRHTILTAYGTSQKVMKRASFIGNISQLTETFAVCIATDYKKQK
                     KIGRTLLIGEEHLHSLEDGSELIDMINK"
     gene            4453..>6056
                     /gene="thsA"
                     /locus_tag="PK196_06955"
     CDS             4453..>6056
                     /gene="thsA"
                     /locus_tag="PK196_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HON41190.1"
                     /translation="MSSLGGQPILILKEGSQRTRGRDAQAANIAAAKAVANAVRTTLG
                     PKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDDEVGDGTTTAVV
                     IAGELLKYAEGLLEQDVHPTVIAHGYRMAADKADEILEGIAVDVRPDDLEMLQKIADT
                     AMTGKGAEAAKGKLTELVVKAITMVADADGSVDTDFVKVEKRVGGSIEDSEIVEGMII
                     DKERVHPAMPRVVKGAKILLLNAPVEFKKTEVDAEISITSPDQLQMFLDEEERMIKGI
                     VDKIVASGANVLFCQKGIDDIAQHYLAKAGVLAVRRVKKSDMEKLARATGASIVSSID
                     AIAPEELGKAGTVEEKKVSGEEMIFVTECDNPKAVSIIIRGGTEHVVDELNRAMEDAL
                     RVVSVAIEDKKFVAGGGAPEIELSLRLRDYAASVGGRAQLAIEAFANALEIIPRTLAE
                     NAGLDPIDMLVSLRASHEQGGATGKYMGLDVYNAVAGNMLDAGVVEPLRVKTQAVASA
                     AEAAIMILRIDDVIAAARSAAPQGGPG"
BASE COUNT         1262 a         1798 c         1767 g         1229 t
ORIGIN      
        1 gagggcatca aaccatgagc gaactggttt gatgggctcg atgagggctc gaggccgtat
       61 ctgcctcaga tcactcatag ggttcgtcat ctccccggcg gggatcagat cggagaactc
      121 atcatcggcg gatgcgggag agacgctcgg ctgcggccgc agggtcgtct gccgcgtaga
      181 tgctccggcc cacgatgacc ccatcgacca gcctggctac cgcatcgagg tcgccgccct
      241 gcgccccgat gcccggtgag tatatcgctt tgtcacccac gatctccctg agcctggtga
      301 ccctttccgg cctcgtggcg ggtgcgataa tgccgtctgc ccggcatctg actgccagtg
      361 ccgcgatgcg ttcggccaca ccatcgtgaa agaactcggt cgcccctgga tggctcatct
      421 cggcgacgat gtatgctgcg ccgctgtggc tgtgcgccac ttcgatacag gccctcgcgg
      481 catcggcccc cacgaacccg tgtgcgatca cggcatcaaa cccggcggag aagaccgcct
      541 cgcatatgag tcggttggtg ttcgggatgt ccgcgacctt gaagtcggcg atgagcggga
      601 ggccagggcc ggcgaggtct tctgtgatcg agagaccggt cgagagaacg agggggtatc
      661 cgaccttgat cgcgtcaatg aacggcgcac aggactcagc gatatttagc gcctctttcc
      721 ggtcagaaac atcaagcgca agtatgagtt cggtcatctc tccaacctct ccagggtagc
      781 ggccacaagc agcggcaggg tgatggtggc atcgccgtag acggtcacgg cggtggcctc
      841 gccggtgagt ttgccccacg atcgtgcctc ttcgagcgtt gcacccgata ggccgccgag
      901 gtcggggcgg tcgccggtga gctgcaccgc atagtcaaac ccggtctcgg tcatcagttt
      961 gctctgcagg atgtagttct tcggaacacc gccgccaaca aggatcgccc cggcccgctg
     1021 tgcttcaaag caccggtcaa gcatccccga catatcggca aacgcgctga cggtgacatt
     1081 gtgcgtctgg gagaagagcc agtactgcag cccgatcatc gagtcctgga tggcagggca
     1141 gtagaccggc acccctgcca ttgccgcttc agccaggatc ccggtcttta gcctgctccc
     1201 gataaggtta aggagatcgg cgatcgagat ggtcgccccc tccgggagag aggagtaaac
     1261 gtcctgcaga aactcctcaa accggataaa tgcttcgttc ggcaggaaga tatcgtagat
     1321 ccggttgatt ccttcttcgt agagctcggt atcggagacc tgcgcgtttc cgtggtagtg
     1381 gtggcagccg atagcctcga tgacgtcgtg tgtcaggttc gcccctgttg agacaagcag
     1441 atcgatgtga cggcgcctta tgaggtcggc aacgattcca cccatcccgc cgggcaccat
     1501 ggcgcccgag agcccaaaaa acttcaacgc cttatcgtcg cggagcattt gctcgtagat
     1561 gttgacagcc tgccagagcg acccaccgtt ataggctcct gccttcccga gttcgtcgac
     1621 gagttcgccg accgtcatcc cggggcgcac ccgtgcctgt tctaccgcat ccccgtagcg
     1681 tgaggtcatg tttgtgttac accaatccct gtagtgctat gaaatgtgtt ggtgctaatg
     1741 cacaaataat ggatcgccat ctctcagaat gtgagtttgc aaaaatgacg tttgggaacc
     1801 ccgatggggc cccgctgcca gacaggggat gactggttat gttatattcg acacgggatg
     1861 atcccactcg tcccaccgtg tcggatgccc gggataaccg gacagtctcg ttagagagtg
     1921 gactttcggg ccggaaacgg aaaggccccc tccggggtta cctttgtcct cgttccgtgc
     1981 ataatcggcc gcgaacttgc acgacccgcc tctgaaaccc gttcgccagg gcggcaactg
     2041 ttgtgggtgg acaggtcagg gcatgcatgc cttcgctgca gacagtcggc acgggatcac
     2101 cagaaggtag gttaactcac aaacattata aatctttttt tgtaaagatc atttgattta
     2161 cttgcaatga ggggatgccg cctttgcgaa tggattgaaa caggttctct tcccacaggg
     2221 ggagaccgcg cgccagaggg cgtgccaggt gcctgcgggc ggatggaggg acctcgtacc
     2281 accccggtct gatatgccgc tcgatctgct ctacctcagg gttgtggcta cgctccacgc
     2341 aggtcctgca cttgtagccc ttccctctcc ctgcagaagt catccttttc ccgcatgccc
     2401 ggcagcgggg agggcggaca cggacggcgt tggcagcgcg tacgactccg atcttctcaa
     2461 ggttcaggct cccgcccttg taactcccgg ccgccatcac gatgtcgccc gggaggagca
     2521 ctctcacaac gtcccggaac ccttttgtcg gttcgtaggc catacagcgg acctcagcac
     2581 cgtcgtcctc aaggatgatc gagacatggc caccccgtcc ggttttaggg ctcccggtaa
     2641 cccgcccgcc taccaggtat gaccgtcctt cccggagggt tccgatcgat ccggcgatca
     2701 ggtgagcatc cgttccctgg ttggttgcat aaacctgctc gcatgccgtc gcctcggatc
     2761 ggacgaacaa acgtgccata tcgacccacg cagggctctc cccacggata ccgaagagaa
     2821 ccgggtcagg ggtgtgtggc acgcagacca ccataccgtt ctcccggtca accgtgtccc
     2881 aggtgtgggg gtatgtcatc tcctcggcgt gaaagaggct tcccgcatcc acacgccgtg
     2941 gagtgcccca ggcctcccgc ttacggtatg caagtaactc ataggtttta tcaggaaatt
     3001 cgcttgcaat cgctgccgtc gcacctataa gtccgcgacg gttcttgtag cctctataga
     3061 gcgcacctgc tgattccaga acacgaacag cctcgtccac ggtgcagaag tcccggagtg
     3121 ctgcgtagta aaaatcaggt ggcggccgga caggtgagac tgccacgcca ggattggtct
     3181 gcgggtctcc gaactccgca agttcttcca cacacactgt agcgagtctg aacgcttctt
     3241 ccagatcccc cacggcctcg atacataccg cagcattacc acgtgtcttg tggatgacgt
     3301 tggggttcag cctgaccagc ctggtatcgg cgacacggat accggcgtgc tccagttgcc
     3361 gcacaagcac cgctccgatg tatgtggtgc acatccctgc aggggaatcc gtgtcgtcga
     3421 tcccgatcca cataacccta tagtttatat tattctccga ctatatcgat tcttaccggc
     3481 catgtcacag gatcgccttt ctcagatggt catcagcatc atgcttcttg ctggcttcga
     3541 cgtctcggag cggtgcaaca tccgcccgag gagttttgac cttatcgcga aaaaaggcga
     3601 cacccttgtc accatcaagg tggtctccca catcgatagc ataaccgccg atacagcctg
     3661 ggatctcaac ctgatcgcca ggtgcctgga ggccaccccc cttatcatag gggagcgggc
     3721 gcgtgatacc gacctggagc gtggcgttgt ctacatccgc tatggactct ttgccatcag
     3781 ccccgagacc ctgtatgatt acctcgtgga aggaaactcg ccgatggtct acgcctctcc
     3841 cggtggcctc tacgtgaaga tcaagggcga tctgctccgg gagatgcggg agcgttccgg
     3901 gatgtctctc ggggatctcg cctcgcacct cggtgtctcc cgcaggacga taagcaagta
     3961 cgagagcggg atgggcacca cccttgagat cgctatcagg ctcgaagagg tcttcaacgc
     4021 tcctctcgtg gagacgatcg atctcattgg ctaccactcg ccagagcccg tgaaaccccc
     4081 agaaccagtg tcaggggata tactctccga tctcgagcgt atcgggatgg agatttatgc
     4141 gatgcggcag gcaccgtttc aggcgctggc gctctttaac aggcacacta tcctgacggc
     4201 atacggcacg tcccagaagg tcatgaagcg cgcttccttc atcggcaaca tctcccaact
     4261 cacggagacg tttgctgtgt gtatcgcgac ggattacaaa aagcagaaaa aaatcggtag
     4321 aacgcttctt attggagagg agcacctcca ttcacttgaa gatggttcgg aactcataga
     4381 catgataaat aagtgaatag atttatatag aactacaaac cacctttgta tcgcttaaca
     4441 acggtgatac ccatgtcaag tcttggagga caaccaatcc tgattctgaa agaaggtagc
     4501 cagcgtaccc gtggtcgtga cgcacaggca gccaacattg cagccgcaaa ggctgtggca
     4561 aacgctgtaa ggacgacact cgggccgaag ggcatggaca agatgcttgt cgacaccatc
     4621 ggcgacgtcg tcattacaaa cgatggtgtg accatcctga aagagatgga tatcgagcac
     4681 cccgccgcga agatgatggt cgagatcgcc aagacccagg acgacgaggt tggcgatggt
     4741 accacgacgg ctgtggtgat cgcgggcgaa ctcctgaagt atgccgaggg tctcctggag
     4801 caggacgtgc accccacggt cattgcacat ggctatcgca tggccgctga caaggcagac
     4861 gagatcctgg aggggatcgc cgtcgacgtc aggcccgatg atctggagat gctccagaag
     4921 attgccgata ccgccatgac cggcaagggc gctgaggccg cgaaagggaa actgaccgag
     4981 ctcgttgtca aagcgatcac gatggtcgct gatgctgacg gcagcgttga caccgatttt
     5041 gtgaaggtgg agaagagggt cggcggctcc atcgaggact ccgagatcgt tgagggcatg
     5101 atcatcgaca aggagcgcgt gcaccccgcc atgcctcgtg tcgtaaaggg tgcgaagatc
     5161 ctgctcctga acgcccctgt tgagttcaag aagaccgaag ttgacgctga gatcagcatc
     5221 acgagccccg accagctcca gatgttcctc gatgaggagg agcggatgat caagggcatc
     5281 gtagacaaga tcgtcgcaag tggtgcaaac gtcctcttct gccagaaggg catcgatgat
     5341 atcgcccagc actacctggc aaaggctggc gtccttgcgg tgcgccgcgt caagaagagc
     5401 gacatggaga aactcgcacg cgccaccggt gcgtcgatcg tcagctccat cgacgccata
     5461 gcccccgagg aactcggcaa ggccggcacc gttgaggaga agaaggtctc cggcgaggag
     5521 atgatcttcg tcaccgagtg cgataacccg aaggctgtct cgatcatcat ccgtggcggc
     5581 accgagcacg ttgtcgatga actcaaccgc gccatggagg atgcgctcag ggttgtaagc
     5641 gttgctatcg aggacaaaaa atttgttgct ggcggcggtg cacccgagat cgagctctcg
     5701 ctccggctac gcgactacgc tgccagcgtt ggcggacgcg ctcagctcgc catcgaggcg
     5761 tttgcaaacg cgctcgagat catcccgaga acccttgccg agaatgcggg tctcgatccg
     5821 atcgatatgc tcgtctctct ccgtgcttcc cacgagcagg gtggcgcgac tggaaaatac
     5881 atgggtctcg acgtttacaa tgctgtcgcc gggaacatgc tcgatgctgg cgtcgttgag
     5941 cctctccggg tgaagaccca ggcagtcgcg agcgccgccg aggctgctat catgatcctc
     6001 cggatcgatg atgtcatcgc tgcagccagg tccgctgcac cccagggcgg cccggg
//
LOCUS       DAOSZX010000088         5990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11154_length_5990_cov_17.693850, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000088 DAOSZX010000000
VERSION     DAOSZX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11154_length_5990_cov_17.693850"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PK196_06960"
     CDS             complement(<1..223)
                     /locus_tag="PK196_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HON41191.1"
                     /translation="MPDFGQPFSGNHMERKLDQGEIVRTIRFVIAAEYEAIQLYTQIA
                     ESTDNQLVKMVMLDIADEEKVHAGEFLRLL"
     gene            complement(302..1207)
                     /locus_tag="PK196_06965"
     CDS             complement(302..1207)
                     /locus_tag="PK196_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HON41192.1"
                     /translation="MQCSKCRREAIVYQRYSGLHLCEQHFNRDFEAKAKRAIREHRWI
                     ESGDTVAVALSGGKDSSAVLFFLHGLLGERRDVRLMAITVDEGIAGYRDPDRARRISE
                     GMGVPWVKASFRDAYGITLDEIVARKGTGLSCTYCGVLRRALMNRVAREEGVTKFAYG
                     FNLDDEAQSVLMNVLRGDAAHLTRPVREVEGLVPRIRPFLYIPEREVALYAFLHVEGF
                     DPAGCPYAVGALRRDVRGILDEYTYRHPATKYSLVRLGEALRRTDAAAGSGFGVCERC
                     GEPMPVKEGKGICRACQVLDELRGA"
     gene            1257..2117
                     /locus_tag="PK196_06970"
     CDS             1257..2117
                     /locus_tag="PK196_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HON41193.1"
                     /translation="MPVPAEYVQSLHPYELRILLALERLMRRYTWVPFEILKSATGFS
                     ESELTYRLGRLINMDMVRYEKTPYDGYALIFDGYDTLALHTLTKRGTIQALGTEIGVG
                     KESEVYEAMGLGVVVLKFHRVGQRSFQAVRLKRGYMPDTGHCPWIFASSNSAKMEYEA
                     LTALHPAVSVPLPIDQNRHVVVMSFIPGVNLTQATLTEPGPILDAILDNISEAYGLGI
                     IHGDLSEFNVMVDQEGQCWLIDWPQWVETAHPNADEILARDITNILQYFKRKYGIDYA
                     FDEALARVVE"
     gene            2114..4063
                     /locus_tag="PK196_06975"
     CDS             2114..4063
                     /locus_tag="PK196_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HON41194.1"
                     /translation="MKVFGIDIIKGSVRSRSRRPVYALCRMEDGEMLDVQEVTAFRLQ
                     RLLTAEQPEILAVDSLQEIAADQHELYAFLQSLPPSTKLVQVTGGERTESLGKVAARY
                     NINFNRFDPYAEARTIARVASLGAGVQVIAFENTTDIVVSRHRSPGPGGWSQNRYARK
                     IHGAVMQKGREIEARLRGAGLDYEKKETKAFGGCSRVAFRVAAPREMVPVHPSRGADV
                     QVRVTGRRLDRIRFEPLSGRPRYLIVGLDPGTTTGIAAVDLDGNLVLLTSSRQMTMSE
                     IVEEIYRAGKPLIVASDVQPMPYSVEKVRRAFNAIAYTPKQSLPVETKYELTALFTYT
                     NDHERDALSAALDAYRSLQSKFRNITKRVGPGFDLDEVRARVLRGQPLDTVIEDMQGA
                     PLPITEAEPAAPAPERSVEDERVMALDGMVKRLRSYVQELQDTLRERDREVEQLRQSL
                     RRARSATERRIRRDAELAAKDAAIESLREQLRGERRRSRRLKKRLERMQAVERLESAE
                     DSTPLKVLDSLTREGVRGLQEDMGIAGGDVLYVPRAHGWGRGVIKDLAAAGVRALLVG
                     GEPPDPNLVRVAREAGLPLIPAEPLSPVLRGKTGVVGSRALDEAIAAWKEEQKEFLRE
                     REAERVEYLVREYRSEREKEVRRGG"
     gene            4056..4922
                     /gene="thiL"
                     /locus_tag="PK196_06980"
     CDS             4056..4922
                     /gene="thiL"
                     /locus_tag="PK196_06980"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HON41195.1"
                     /translation="MDERGLHRLIGTIIAPEHLEDDCAVIPCADRIVVASTDMLHETT
                     DFPAGMTDWQAGWMAVAVTLSDVASMGAAPGQVLLAVGLDRPERLRGIMEGARDCCAA
                     FGAELVGGDLDAHSELTLVSTGIGWVEPGYLVRRRGARPGDAVAVTGRLGEAQAALNG
                     WEQHRRELLEPQPRVREGQALARAGVSAMMDISDGLALSLYDLLAVNDCGFAIDTARL
                     PLPAGVPVEEGRELALYGGGDFELLFCAPQGVLPVAGVEAHVIGEVIDERVVLADGEP
                     LEPRGYLHEWSG"
     gene            5058..>5990
                     /locus_tag="PK196_06985"
     CDS             5058..>5990
                     /locus_tag="PK196_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HON41196.1"
                     /translation="MNQREVEPVPQGGGRFSNNFDFMRFAAATLVVAAHAYALHTGYS
                     QIGLHDPVMLAGRAALAALLATSGYLIAASWESTASPLRFAWKRFLRVVPGLIPVILV
                     TLFILGPLVTSLPQDDYFATLLSPEGFAAVPFFENGGVLGLFQDNPVTYVNGSLWTIP
                     LEAALYGVVAALGVAGLLHRRGALPALIALNVLVWFCWFDDVRMAKVRFSLYFLVGAL
                     LYVRRERVTLRPAIAGGLLILLVASAMTPLAAIAGVVAIPYLTIYAAHIPLPLLNTFG
                     RHGDFSYGIYLYHYPLQQTIIQVTANTVAIPALFG"
BASE COUNT         1064 a         1889 c         1914 g         1123 t
ORIGIN      
        1 gcagaaggcg caggaactca cctgcgtgaa ccttctcctc gtctgcgata tcgagcatga
       61 ccatctttac aagctggttg tcggtcgatt cagcgatctg ggtgtagagc tggatcgcct
      121 catactcggc ggcgatcaca aacctgatcg tcctgacgat ctcgccctgg tcaagtttgc
      181 gctccatgtg gttcccggag aacggttgtc caaaatcagg cataatcaat tacctccagt
      241 cgttcatggg cgaggggatt atttataccc accgccggac ggagagcgac ccccctgtcc
      301 atcaggcccc tctcagttca tcaagaacct ggcacgcccg gcagatccct ttaccctctt
      361 ttaccggcat cggttcgccg cagcgttcac agacgccaaa cccgcttcct gcggctgcgt
      421 cagtcctccg gagggcctca cccagcctca ccagcgagta cttcgttgca gggtggcggt
      481 aggtatactc gtccaggatc ccccgcacat cgcgcctgag ggcgccgacc gcgtaggggc
      541 agcccgcggg gtcgaacccc tcgacgtgga ggaaggcgta gagcgccacc tcccgttccg
      601 gaatgtagag aaacggccgg atccgcggga ccagcccctc gacctcccgc accggtcggg
      661 tgaggtgcgc cgcatccccc cgcaggacgt tcatcagcac ggactgggcc tcgtcgtcca
      721 ggttaaaccc gtatgcaaac ttcgtgaccc cctcctccct ggcaaccctg ttcatcagcg
      781 cccgccggag cacgccgcag taggtgcagg atagcccggt gcccttcctg gccacgatct
      841 catcgagtgt tatcccgtag gcgtcgcgaa acgaggcctt aacccaggga acacccatac
      901 cctcagatat cctcctcgcc cggtcagggt cgcggtaacc tgcgatacct tcatccaccg
      961 tgatcgccat caaccggacg tcccgccgct cgccaaggag cccgtggaga aagaagagaa
     1021 cggcgctcga atccttgccg cccgagagcg ccacagccac cgtgtccccg gactcgatcc
     1081 agcggtgctc cctgatcgcc cgtttcgctt tcgcctcaaa atcgcggttg aaatgctgtt
     1141 cgcagaggtg cagccccgag tagcgctggt agacgatcgc ctcacgccgg catttgctgc
     1201 actgcatcct ctctcgatca tacttttatc accttaccac ataaattctg tagacgatgc
     1261 ctgttcctgc agaatacgtg caatccctgc acccctacga actccggatc ctgcttgccc
     1321 tcgagcgtct catgcggcgc tacacctggg tgccgtttga gatactcaaa tctgccacag
     1381 ggttctcgga gtccgaactc acctaccgtc tgggcaggct gataaacatg gacatggtcc
     1441 gttacgaaaa gaccccttac gacggctacg ccctgatctt tgatggctac gacaccctcg
     1501 ccctccatac cctgacaaag cgcggcacca tccaggcgct cggaacagag atcggtgtcg
     1561 ggaaggagtc ggaggtctac gaggcgatgg gtctcggggt cgtggttctg aagttccacc
     1621 gcgtcggcca gcggtctttc caggcggtac ggctcaagag agggtatatg ccggataccg
     1681 ggcactgccc ctggatattt gcctcgagca actcggcaaa gatggagtat gaagccttaa
     1741 cggccctcca ccccgccgtc tccgtcccgc tccccatcga ccagaaccgg cacgtggtcg
     1801 tgatgtcttt catccccggt gtcaacctca cgcaggcaac cctcaccgag cccgggccga
     1861 tcctggacgc gatcctggat aacatcagtg aggcctacgg tctcgggatc atccacggcg
     1921 acctgagcga gttcaacgtc atggtcgatc aggaggggca gtgctggctg atcgactggc
     1981 cgcagtgggt tgagaccgcc cacccgaacg ctgacgagat cctggcacgg gacatcacaa
     2041 acatcctcca gtacttcaaa cgaaaatacg gcatcgacta cgccttcgac gaagcactgg
     2101 cacgggtggt ggagtgaagg tcttcgggat agatataatc aagggctcgg tacgctcgcg
     2161 cagccgccga ccggtctacg ccctctgccg tatggaggat ggcgagatgc tggacgtcca
     2221 ggaggtcacg gcgttccggc tccagcgtct ccttaccgcc gaacagcccg agatcctggc
     2281 ggtggacagt ctccaggaga tcgctgccga tcagcacgaa ctctacgcgt tcctccagtc
     2341 gctcccgccc tcaacaaaac tcgtccaggt gaccggcggc gaacgcacgg agagtctcgg
     2401 gaaggttgcc gcccgctaca acatcaattt caaccggttc gacccctacg ccgaggcccg
     2461 gacgatcgcc cgtgtggcct ctctcggtgc gggcgtccag gtgatcgcgt ttgagaacac
     2521 caccgatatc gttgtgagca ggcaccgctc gccaggcccc ggcggctgga gccagaaccg
     2581 ctacgcaagg aagatccacg gtgcggtgat gcagaagggg agagagatcg aggccagact
     2641 ccgtggtgcc gggctcgatt acgagaagaa ggagacgaag gcctttggcg gctgctcccg
     2701 tgtcgcgttc agggtcgccg ctcccaggga gatggtcccc gtccacccct cacgcggcgc
     2761 cgatgtccag gtccgggtga cggggaggcg ccttgaccgg atccggtttg aaccgctctc
     2821 cggccggccg cgttacctga tcgtggggct cgatcccggg acgacgaccg ggatagctgc
     2881 cgttgacctc gacggcaacc ttgtcctact cacgagttcc cggcagatga cgatgtcgga
     2941 gatagtcgaa gagatttacc gtgctggaaa accgctcatc gttgcatccg acgtccagcc
     3001 gatgccgtac tcggtcgaga aggttcgacg ggcgttcaac gccatcgcct acaccccgaa
     3061 acagtcgctc ccggtggaga cgaagtatga actgaccgca ctgtttacat acacaaacga
     3121 tcacgagcgt gacgctctct ctgcggcgct ggacgcctac cgcagtctcc agagcaagtt
     3181 tcggaacata acaaagcggg tggggccggg gttcgacctt gacgaggtga gggcccgggt
     3241 tctgcggggt cagccgctcg ataccgtcat tgaggatatg cagggcgcgc ctctgcctat
     3301 aaccgaggct gaaccggcgg cccctgcccc ggaacggtcg gtcgaggatg agcgggtgat
     3361 ggcgcttgac gggatggtca agcggctccg cagttatgtg caggagttgc aggacactct
     3421 ccgggagcgc gaccgtgagg tggagcagtt gcgccagagc ctgcgccgcg cacgctccgc
     3481 gacggagcgc aggatccgcc gggatgcgga actggcggca aaggacgcgg ccatcgagag
     3541 cctgcgggaa cagctgcggg gcgaacggcg gcggtcgcgg cggttgaaga aacggctgga
     3601 acggatgcag gcggtcgaga ggcttgagtc agctgaagat tcaacacccc tcaaggtgct
     3661 tgactcgctc acgcgggagg gggtgcgggg tcttcaggag gacatgggca tcgctggagg
     3721 tgacgtcctc tacgttccac gggcgcatgg ctggggccgg ggcgtgataa aggacctggc
     3781 tgcggcgggg gtgcgggcgc tcctcgtcgg cggggagccg cccgacccca atctggtcag
     3841 ggttgcccgg gaggcgggtc tccccctcat cccggcagag cctctcagcc cggttctccg
     3901 ggggaagacc ggtgttgtgg ggtcacgggc gctcgatgag gcgatagcgg catggaaaga
     3961 ggagcagaag gagttcctgc gggagcggga ggctgaacgc gtggagtatc ttgtcaggga
     4021 gtaccggagc gaacgggaga aggaggtgcg ccgcggtgga tgaacggggg ttgcaccggc
     4081 tgatcgggac gataatcgcc ccggaacacc tggaggacga ctgtgcggtc attccctgcg
     4141 ctgaccgcat cgtggtcgcg agcaccgata tgctccacga gacgaccgat tttcccgccg
     4201 ggatgaccga ctggcaggcg ggctggatgg cggtggcggt cacgctctct gatgttgcaa
     4261 gcatgggcgc ggcgccgggg caggtgctcc tggcggtagg tctcgaccgg ccggagcgtc
     4321 tccgcgggat catggagggc gccagggact gctgcgccgc attcggcgcc gaacttgtgg
     4381 gcggcgacct tgacgcccac agcgagctga cgctcgtcag cacggggatc gggtgggttg
     4441 agcccggata tcttgtccgg cggcgggggg cgcggccggg ggacgccgtc gcggtgaccg
     4501 ggcggctcgg ggaggcgcag gccgccctga acggttggga gcagcaccgc agggaactcc
     4561 tggaaccgca gccgcgtgtc cgcgaggggc aggcgctcgc ccgtgccggg gtctcggcga
     4621 tgatggatat atccgatggg ctggctctct cgctttatga tctcctcgcc gtgaacgatt
     4681 gcgggtttgc catcgatacc gcaaggctcc ccctccccgc cggggtgccg gtggaggagg
     4741 ggcgtgaact cgcgctctac ggcggcgggg attttgaact cctcttctgc gccccgcagg
     4801 gtgtccttcc agtcgccggt gttgaggcgc acgtcatagg cgaggtcata gacgagcggg
     4861 tcgtccttgc agacggggaa ccgcttgaac cccgcggtta cctgcatgag tggagcgggt
     4921 aggtctcctc ttctggatct ccaggtcact ggaggggcgg gcgccggcgg tgatcaggag
     4981 aagttataaa gcctgctgat ccaggtagtc tcgatagacg gctggctatc ttttacgttt
     5041 gatcatcaac tcgaggcatg aatcagagag aggtggaacc ggttccgcag ggcggcggcc
     5101 ggttctcgaa caacttcgat ttcatgcggt ttgccgcggc aacgctggtt gtcgctgctc
     5161 acgcatacgc gctccatacc gggtattcgc agatagggct gcatgacccg gtcatgctcg
     5221 ccggacgggc agcgcttgcc gccctccttg ccaccagcgg ctacctgatc gcggcgagct
     5281 gggagtcgac cgcatctcca ctcaggttcg cctggaaacg gtttctccgg gtcgtcccgg
     5341 gtcttatccc ggttatactg gtcaccctct ttatcctggg cccgctggtg acgtccctcc
     5401 cgcaggatga ctactttgcg accctcctct cgccggaggg gtttgccgcc gtgccgttct
     5461 ttgagaacgg cggggttctg ggtctcttcc aggacaaccc tgtaacctac gtcaacgggt
     5521 cgctctggac gataccgctg gaggctgcgc tgtatggggt tgttgcggcg ctgggggtcg
     5581 ccgggctcct gcaccgccgg ggtgcgctcc ccgccctcat cgctcttaac gttctggtct
     5641 ggttctgctg gttcgatgac gtccggatgg caaaggtccg gttctcgctc tacttccttg
     5701 tcggggcgct tctctacgtt cgccgtgaac gggtcacgtt aaggccggca atcgcgggtg
     5761 ggctgctcat actccttgtg gcatcggcca tgacgccgct tgcggccatc gccggggtgg
     5821 ttgccatacc ctacctcacc atctacgccg cgcacatccc tctccccctc ctaaacacgt
     5881 tcgggagaca cggggacttc tcgtacggga tctacctcta ccactatccg ctgcagcaga
     5941 cgatcatcca ggtcacggca aacaccgttg ccatcccggc gctcttcggg
//
LOCUS       DAOSZX010000089         5935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11293_length_5935_cov_15.533673, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000089 DAOSZX010000000
VERSION     DAOSZX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11293_length_5935_cov_15.533673"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK196_06990"
     CDS             <1..182
                     /locus_tag="PK196_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HON41197.1"
                     /translation="EVSLMPGRTAEKKEALFRAIVGSLGRSPGIRPEDLCIVLYEPPV
                     ENWAEHGERFAAPPG"
     gene            800..1255
                     /locus_tag="PK196_06995"
     CDS             800..1255
                     /locus_tag="PK196_06995"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON41198.1"
                     /translation="MNGSPTDGSILLTRPRQLVNRFGTIVFEDLNITNIQKNHHLEKS
                     IADAAWSMFIKITRSKAEEAGSHAILVNPRNTSQMCSRCGALVAKTLSDRVHSCPYCG
                     LVMDRDQNAGVQYYEIGAAISGVTSQDAPDERRGSSHICDWKKEGGWRV"
     gene            complement(1337..2764)
                     /locus_tag="PK196_07000"
     CDS             complement(1337..2764)
                     /locus_tag="PK196_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HON41199.1"
                     /translation="MNETFSNFVKEVKAIVGDANATDDRAMCTSYSRDQHWHFVPAKN
                     PACVVRPGNKEEVRAVLMLANKEKIPVVPYSTGINVRGLTIPVHDGSILLDLSRMNRI
                     LEINPDMKTATVEPGVTFGMLFDKTKEYNLRPAFPDAPLTVSVLANYYLRGIYQTSAT
                     DGHDHTISYEMLLPNGETLKTGSRALSDMPYFRYGVGPDFAGMFCAHPGTCGVVTELT
                     AKLYRLYDNRKGYFIGFDDVGTPTKFIYNLMNDELAASIRLVDNQSWLKGIFGTFDYP
                     YEDLPKFVVCVLVEGVDGIFDAKDNLVREYMNESGGKVLELPAEFARTFEEESLGGAE
                     RSCMVFGLKGKGNYHCIGLYGPLTRASEYYEVHEKTALEVGIPDDHIHFYVNPIHGFH
                     GQVSYFELDVFYDGSDAEFGEKLRNYNEKQFHRLLDIGIYGWFRPYAGIIEPTVERIG
                     YLAEVWKKFLHVLDPNEIMNRGKLF"
     gene            complement(2770..3897)
                     /locus_tag="PK196_07005"
     CDS             complement(2770..3897)
                     /locus_tag="PK196_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HON41200.1"
                     /translation="MEPKRKKEVLSAIHHCVKCKLCSISSFHPNAEWLPLCPSGQYYG
                     YQAFYAPGKMEIFRAILEGEITEPSGGLLNSVYACTVCGACYEKCKQITSVELGAPEL
                     FEEMRCELAGKGWILDAHKSIAEKIRETKNAYGEKYEYKAESTGGNADVLYYIGCTAR
                     YRVPEIADAMLEIFDKLGLKYQVMGEEWCCGSVLFRTGQVEAAKELVAHNIEGIKRSG
                     AKTVVFTCPGCLKTFKKNYPDMGVELVHSTQFLTRLGSMNLKKQDMKAAYHDPCHLGR
                     DLGIYEEPRAVLNEVTNIKAMKREREQAWCCGAGGGVKSAFGDFALWSAAERLKEAKE
                     AGAEALVSACPFCKRNLKEAAKKEELEVYDVVEIVNRCLEG"
     gene            4059..4208
                     /locus_tag="PK196_07010"
     CDS             4059..4208
                     /locus_tag="PK196_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41201.1"
                     /translation="MFEYGYTRDNPSLPRINLSLIMDRDTGDVSTTPAFRTGSYPCSA
                     VMKSS"
     gene            complement(4200..4589)
                     /locus_tag="PK196_07015"
     CDS             complement(4200..4589)
                     /locus_tag="PK196_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41202.1"
                     /translation="MRQDTFGAYPPEPLDGGGRKPHDPSRFRPVLHRSPDGLTGDLRD
                     EEDAKRRYHSLSDRLNEEVEPLEKTAALYDAAKADLAAILKERGVETPEDYREIERLP
                     RERQKLEESVRDAEVRDQEDQRGGALR"
     gene            4709..5293
                     /locus_tag="PK196_07020"
     CDS             4709..5293
                     /locus_tag="PK196_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HON41203.1"
                     /translation="MSPLTYPIGEEFKPVPAYRTYLYTSLLISVVVLILPWLIPTVIF
                     APRVVGLGLGIPTVAVIIFVAWWIPLYCESITYRLTPTEITWERGVWFRQTGIVPYNR
                     ITNVDISQGPLMRLFSFSSLRVQTAGYSGQARSEIVLTGIADPKDLQETIMGFVRKSG
                     PAAAEGGPERAAEIGAVVEELRAIRNLLEAGQRR"
     gene            complement(5328..5801)
                     /locus_tag="PK196_07025"
     CDS             complement(5328..5801)
                     /locus_tag="PK196_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HON41204.1"
                     /translation="MFERILFPTDFSEPSMKVLDYIPALHEAGTREIILVHVIDSKDI
                     GLGASGGQGFLGTVSNQETEAHRQLQTEVQHRIIDTRRALEKQGFEVTVLTPVGNPGK
                     EIVAAADAEGASLIVLGSHGRSNIRDRLLGTVSEYVIKNANQPVLVIKRGKETGR"
BASE COUNT         1320 a         1778 c         1392 g         1445 t
ORIGIN      
        1 tcgaggtcag ccttatgccg gggcgcaccg ccgagaagaa ggaggccctc ttccgcgcga
       61 tcgtcgggag ccttggccgt agccccggga tccgccctga agatctctgc atagtcctct
      121 acgaaccgcc cgtcgagaac tgggccgaac acggcgagcg gtttgccgcg cctccgggat
      181 gagcctcacc cccaccgggg cttcataccg atctgccggt cggctgtgtg cgaggagcct
      241 tgccatctct ccgggatggc ggggctggct ggaggtgtcc ggatagagat ataatttttt
      301 atcaggccgt gaagatattc aaagtcgtga atacaaatat tcccggaaac tgttcttttc
      361 ccgaagagtt agggtttctg gactctgcaa gcgctcacac cggacgcact atcatgttta
      421 gagaactttt gctgctcgaa gttactccac atgagatctc attcgatgag atccgcagga
      481 acctacattc ccggaaacac ataacgagtt caataatcgg ccttattatc atcctttaaa
      541 tcctcatcgc tcttcaaaat caaagtaata ggtttagcca tcgttatgtg ttaacacata
      601 aagagaacag ttgcatttgt caacggaatc taccccgttt aggagtcctt tgccaccctc
      661 tcaaacgggg agaagatcga gaacccccgg ttcttccgca ccgatgagaa ggctcttgca
      721 aaggcgcaga gaaggctttc aaaagcagag aaaggcactc ctgaacggaa gaaagcccgg
      781 aagatcgttg cacacgtcca tgaacggatc gccaacagac ggctcgattt tgctcaccag
      841 accccgacag ttggtgaatc ggttcggtac gattgtgttc gaggatctga atatcacaaa
      901 cattcagaag aaccatcatc tggaaaaaag cattgcagat gccgcctgga gtatgttcat
      961 caagatcacc aggagcaaag ctgaagaggc tggctctcac gcgatcctgg tcaaccccag
     1021 aaacacatcg cagatgtgct ccagatgtgg cgcactcgtc gccaagacgc tctctgatcg
     1081 cgtccattcc tgcccgtatt gcgggctggt gatggaccgc gaccagaacg cgggcgttca
     1141 atattatgag attggggctg caatctcagg ggtaacatcc caggatgccc ccgacgagag
     1201 gagggggagc agtcacatat gcgattggaa aaaagagggc gggtggaggg tatagcccct
     1261 gaatcatgct gtgtttgggt tactaagagc atataacgga tttttcatcc ctcatggcag
     1321 tctcgtacag cagtcttcag aacagcttcc ccctgttcat tatctcgttt ggatccagaa
     1381 catggaggaa cttcttccag acttcggcaa ggtaccctat tctttccact gtcggctcta
     1441 ttatacccgc gtatggtctg aaccagccgt agatgcctat gtcaagcagc ctgtgaaatt
     1501 gcttctcgtt ataattcctc aacttctcac cgaattccgc atcacttccg tcgtaaaaca
     1561 catcaagctc gaaatagctg acctgcccgt ggaaaccgtg tatcgggtta acatagaaat
     1621 gaatgtggtc atcgggtata ccaacttcca gtgccgtctt ctcatgcact tcataatact
     1681 ctgatgcccg tgtcaatggt ccatagagcc cgatgcaatg atagttcccc ttccccttca
     1741 agccgaacac catacaactc ctctctgcac cgcccaagct ctcctcctcg aacgttcgcg
     1801 caaactcagc cggtaattca agcacctttc cacctgattc gttcatatac tcacgaacca
     1861 ggttatcttt cgcatcaaat ataccgtcca caccttctac cagcacacag accacgaact
     1921 tcggcagatc ttcataagga taatcaaaag tgccaaagat gcctttcaac cagctttgat
     1981 tgtccactaa ccggatactt gccgctaatt catcgttcat taaattgtaa ataaactttg
     2041 ttggcgtgcc cacatcgtca aaaccaatga agtagccttt cctgttgtca tacagccggt
     2101 aaagtttcgc tgttaattcg gtcaccacac cgcacgtacc tggatgtgcg cagaacatac
     2161 ctgcaaagtc cggtcccaca ccgtaccgga aataaggcat atcagaaagc gctcgtgagc
     2221 cggtttttaa tgtctcgccg ttcggtagca gcatctcata cgagatcgta tggtcatggc
     2281 cgtccgtagc cgaagtttga taaatcccac gcaaatagta attcgccagt acgcttaccg
     2341 taagcggcgc gtccgggaat gcgggtcgga ggttgtattc ctttgtctta tcaaataaca
     2401 tcccgaaggt cacacctggc tctactgttg cagtcttcat atctggattt atctctaaga
     2461 tgcggttcat ccttgacagg tctaacaaaa tactgccgtc gtgaaccggg atggtcaagc
     2521 cacgcacatt tattcccgtg ctgtacggca ccaccgggat tttctcctta tttgcaagca
     2581 tcaaaaccgc tcgcacttct tccttatttc ctggtcgcac gacacaagcc ggatttttcg
     2641 cgggcacaaa atgccagtgc tgatctctcg aatacgatgt gcacatcgcc ctgtcatcgg
     2701 ttgcgttcgc atctcctact atcgccttca cctcctttac aaaatttgag aacgtttcgt
     2761 tcatcttaat cacccctcta agcacctatt tacaatctcc acaacatcgt acacttccag
     2821 ttcttctttc tttgcagctt cctttaaatt ccgtttacag aacgggcatg cactcaccag
     2881 tgcttcagca cccgcttctt tcgcctcttt caaccgttca gcagcactcc atagtgcaaa
     2941 gtcaccaaat gccgacttca caccgccacc ggcgccacag caccatgctt gctccctttc
     3001 acgcttcatc gcctttatat tggttacttc gttcaaaacc gctcgcggct cttcatatat
     3061 acccaaatcc ctgcctaaat ggcatgggtc gtgatacgct gctttcatat cctgcttctt
     3121 caggttcata cttcccaatc gtgttaaaaa ctgcgtagaa tgcaccagtt caacacccat
     3181 atcgggataa ttcttcttga atgtcttcag acaaccggga caggtaaaaa caacggtctt
     3241 tgcgccactc cgctttatac cttcaatgtt gtgtgccaca agttccttcg ccgcttctac
     3301 ctgtcctgtt ctgaataata ccgagccaca gcaccattcc tcacccatca cctgatattt
     3361 cagccctaat ttatcgaaga tttcaagcat ggcatctgca atctccggca cacggtagcg
     3421 tgcggtacag ccaatgtaat agaggacatc cgcattcccg ccggttgatt ccgccttgta
     3481 ttcatatttc tcaccatacg cgttcttcgt ctctcttatc ttttccgcaa tggatttatg
     3541 tgcatcgaga atccagcctt tacctgccag ttcgcaccgc atctcttcaa acagttctgg
     3601 tgctcccagt tccacacttg ttatctgctt acacttctcg tagcacgcgc cgcatactgt
     3661 acaggcatag accgaattca atagcccccc cgaaggttct gttatctcgc cttctaatat
     3721 cgctcggaag atctccatct tacccggtgc gtaaaacgct tgatagccgt aatactgtcc
     3781 gctggggcat aacggcaacc attcagcgtt tggatggaaa ctcgaaatgc tgcagagctt
     3841 gcatttcaca cagtggtgaa ttgcgctcag tacctctttt ttccttttcg gctccatgtt
     3901 tattacacca ccctctgctc ggaactataa ataccttcgt cgttggcagg agcaacaccc
     3961 cggatctcct ggtggccggg cttcttgcgg agagcaaaac ccaacaaacc ctggtgcatg
     4021 acatcaccag cctctccagt tgctcatatc tgatgcaaat gttcgagtat ggttataccc
     4081 gggacaaccc gagtctgccc cggatcaacc tctccctgat catggacagg gacaccggcg
     4141 atgtgtcaac tacaccggct ttcaggacag ggtcgtaccc ctgcagcgcc gtgatgaagt
     4201 catcgtagcg ctcctccccg ttgatcctcc tgatcgcgga cttcagcatc ccggacgctc
     4261 tcctcaagtt tctgtctctc ccgtggcagc cgctcgatct cgcggtaatc ctccggtgtc
     4321 tccacgccac gttccttcag aatggcggcg agatccgctt ttgctgcatc atacagagcg
     4381 gccgtcttct ccagcggttc cacctcctcg ttcaaccgat cactgagcga atggtatcgt
     4441 ctcttcgcgt cctcctcatc gcggagatcg ccggttaaac catcggggct cctgtgcaga
     4501 accggccgaa aacgggatgg gtcatgaggc ttcctgccgc cgccatcgag gggttcaggg
     4561 ggataggcgc cgaaggtgtc ttgcctcacg gccgcaccgg agaaccgcgg cgccacgagc
     4621 agcgattcac ggccggccgc tgcattcccc ggagatgccc tggcgatcgg aaaggcttaa
     4681 ccctgctggg ttgaaaactt ttcataccat gtcccctctc acctacccta taggcgaaga
     4741 gttcaaaccg gtgccggcgt accgcaccta cctctacact tccctcctga tcagtgtcgt
     4801 cgtcctgatc ctgccctggc ttatcccgac cgtgatcttc gccccgcgtg tggtcggcct
     4861 cggccttggc ataccaacgg ttgccgtcat aatctttgtc gcctggtgga tacctctcta
     4921 ctgcgagagc ataacctacc gcctcacccc gaccgagatc acctgggagc gtggtgtctg
     4981 gttccggcag accgggatag tcccgtacaa ccggatcaca aacgtcgata tctcccaggg
     5041 gccgttgatg cggttattct ctttctcatc cctccgggtg cagaccgccg gctactcggg
     5101 gcaggcgcgc tcagagatcg tcctgaccgg gatcgccgac ccaaaagacc tccaggagac
     5161 gatcatgggg tttgtccgga aaagcggccc cgctgcagcc gaaggcggac ccgaaagggc
     5221 ggcagagatc ggtgcggtgg tggaggaact gcgggccatc cggaacctgc ttgaggccgg
     5281 acagaggaga taagagagcc tccaggcggg gaaaagggtg cccccgccta tctccctgtc
     5341 tccttccccc gcttgatgac caggacgggc tgattggcgt tcttgataac gtactccgag
     5401 accgtcccga gcaggcggtc ccggatgttt gaccggccgt gagagccaag gactataagc
     5461 gatgctccct cggcatcggc ggcggccaca atctcttttc cggggttgcc gacgggcgtc
     5521 aggacggtca cctcaaaccc ctgcttctca agcgcccggc gggtatcgat tatccggtgc
     5581 tggacctcgg tctgcagctg ccggtgggcc tcggtctcct ggtttgatac cgtcccaagg
     5641 aacccctgcc cgccggaggc gccaagcccg atatcctttg agtcgattac gtggacaagg
     5701 atgatctcac gggttcccgc ctcgtgcagt gcggggatgt agtccagaac cttcatcgag
     5761 ggctctgaga agtccgttgg aaacaggatg cgttcaaaca tactctggca ctctctccag
     5821 gtgtagtctt ttctatcagg ggtataaaat ggattctgta cacccctttc agccgcgtct
     5881 cctatcccgg atgagcaccc tccaccggtg cagcatccgg ccgacccctc ccgtc
//
LOCUS       DAOSZX010000090         5770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11680_length_5770_cov_19.716010, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000090 DAOSZX010000000
VERSION     DAOSZX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11680_length_5770_cov_19.716010"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK196_07030"
     CDS             <1..181
                     /locus_tag="PK196_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41205.1"
                     /translation="GYVNEIGTAGWYGSLAFFVVHAAVYLASILLILKSDPTLYDDTR
                     SAVGQVVSRIRRRAG"
     gene            complement(389..1555)
                     /locus_tag="PK196_07035"
     CDS             complement(389..1555)
                     /locus_tag="PK196_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HON41206.1"
                     /translation="MDVARLASSLIRIRSENPPGNTTEIVAFIAEFLDSLGVKNRIVS
                     NPDGRDNLVTTEPGPQLLLCGHVDVVPAIPDDWLYDPFSGTIADGYVWGRGATDMKGG
                     CAALLVACRDLIESGVEPNVQFAFVCDEETGGEHGIRSLLAEGLLKPRECLIAEPTPH
                     TSPAIGQKGLYRLDISFRGTPGHSSLYPLVGRSAIMAAFDLLGYLREVHAHQFPVDED
                     LQPLISQSAALFREIFGIEGGDSILTRVMFNPGRIEGGEKANIVAEQCRMDLDIRVPW
                     GCLLDDLRRGIAEHAPDAVVREVDVAEPTLTPPGARVVRTVCAEVERVHGTAAPFLQW
                     AASDAKYLRDEGFDVVEYGPGEITTLHAVDERVSIEQLERAVDVYRGVIRAYSG"
     gene            complement(1568..2077)
                     /locus_tag="PK196_07040"
     CDS             complement(1568..2077)
                     /locus_tag="PK196_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41207.1"
                     /translation="MLETNEYVRVMEKLYNKSLILESPSDFHPVLHFYFTDALAHIDF
                     TATVLAYNPMSPRNIMSMEYMRWRLDQEKVGDRVHFPGFINWLKTEHPEVYEELPMLW
                     TGIYDTDDPAQYRSFRIVLNPDERGPIPSEYLSMFIDEFFDPAFIKQLYKGSSLARLF
                     DEYAAARSE"
     gene            complement(2172..3047)
                     /gene="moaA"
                     /locus_tag="PK196_07045"
     CDS             complement(2172..3047)
                     /gene="moaA"
                     /locus_tag="PK196_07045"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HON41208.1"
                     /translation="MLKDPYNRTVTNLRISLTSRCNLRCIYCHAEGEVNPKEQMSADE
                     IAELMRIGAQFGIRSVKFTGGEPMLRRDLVDIIRSVPPGVESSMTTNGTLLAPKAAEL
                     KEAGLARVNVSLDTLRPERYSRITGKDCLEDALEGIDAALQAGLTPVKINMVLLDGIN
                     EDEIDDFMAFVRGKRDLVLQVIELMEFNECRFHGDVDSVEQELNERATRVVTRRMHHR
                     KKYCLDGAEVEVVRPLHNTEFCAFCNRLRVTSDGNLKPCLLRTDNLVNIRGKHGKELE
                     DAFREAVSRRRPYFT"
     gene            complement(3028..3639)
                     /locus_tag="PK196_07050"
     CDS             complement(3028..3639)
                     /locus_tag="PK196_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HON41209.1"
                     /translation="MGSQWTKDSVYRKAMQSGYRARAAYKLLEIQQRNGVIRADDNVV
                     DLGAAPGSWLQVLRDLTDGRIIGVDLNPIAPMEGVITLVGDFTDPPVQERIRQEAGGT
                     VNVVVSDASPKLSGQKSYDQARAIGLGEDALAFACTILKPGGNMVLKSFQGEFFGELV
                     AEARKHFYSVRSYRTKASRKGSAEIYIIAKNFKGACNDAEGPL"
     assembly_gap    3806..3905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4023..4766
                     /locus_tag="PK196_07055"
     CDS             4023..4766
                     /locus_tag="PK196_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HON41210.1"
                     /translation="MLKATIDAEIFRESIDAIAALVTECRLHTQEDMIWTRSVDTANV
                     AMVTLELQSTAFTSFSATGGELGLDVAKLKNILAMIGKGDALTLQLQDNDRKLELSFG
                     GYRYSITLLDVNTIRKDPNPPAVNLPGKAVIPGETLNNAIKAASVISDKIALGIDPES
                     MTFYMEAEGDTDHIKLALSEDELIALNPVKARSLFSIDYLKDMGRVMARAEKVEIHLG
                     INHPVRFVFEIADGNGRVDYLLAPRVEAD"
     gene            4766..5770
                     /gene="priL"
                     /locus_tag="PK196_07060"
     CDS             4766..5770
                     /gene="priL"
                     /locus_tag="PK196_07060"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HON41211.1"
                     /translation="MDIVLDRKELTKYPFLKESQQLASQHVESLEAFLASSAGNVALE
                     RAKERIIAALRFRRDFQDDAARGPAPELEIASYALARIIVSCIGDRSLIDRLARYESD
                     RSSFFLAAEEPEVRRFVALRLQIDPDARSMPVTDYVELAARLRDNRWRLVNRDVNQGV
                     VGVEPGEIDELIRERIRTIVADNLPLRVPGGICETLAAVASEITALQQQQALEEFGEI
                     REEAFPPCIASLLQALTAGTNLTHMGRFAITSFLHTIGMNIAQIAEIFAQAPDFDPDM
                     TMYQVEHISGRGGTEYTPPSCITMRTFGLCVNRDKECERVSHPLSYYRLKKSGAKKQV
                     "
BASE COUNT         1257 a         1719 c         1503 g         1191 t
ORIGIN      
        1 gggttacgtt aatgaaatag ggacagccgg gtggtatggt tcgctcgcgt tctttgtagt
       61 ccatgctgca gtctatctgg catcgatcct tctcatacta aagtcagatc caaccctata
      121 tgatgacacc agatctgcag ttgggcaggt agtatcaagg atcaggaggc gcgcgggata
      181 gtccaaaacc tggtgaataa acgatctaca ggtggtagat cgtgtcagct tgatcaccca
      241 aaataaagga cagtgttact gaaatgtgtc ggaacatcca tttggtgcac tcataaactc
      301 accgggaacg acgcgcactc agggtgtacc cccctggcag tttcctcttc tatcatcaat
      361 aatcacccgc gccactcccc aatctccctc accccgagta tgcccggata accccacggt
      421 atacatccac cgcccgctca agctgttcaa ttgacacccg ctcatccacc gcgtgcagcg
      481 tcgtgatctc ccccgggccg tactccacaa catcgaaccc ctcgtccctg aggtacttcg
      541 catcgcttgc agcccactgc aggaacggcg ccgccgtccc atggacgcgt tccacctcag
      601 cacagaccgt ccggacaaca cgggcgccag gcggcgtcag ggttggttcc gccacgtcca
      661 cctcgcgtac cacggcatca ggagcatgtt cggcgatacc gcgccggagg tcatcgagaa
      721 ggcaccccca cggcacccgt atatcaaggt ccatccggca ctgctccgcc acgatgtttg
      781 ccttctcacc accctcgatc cgccccgggt tgaacatcac ccgtgtcagg atgctatccc
      841 ctccctcgat cccgaagatc tcgcgaaaga gcgcggcaga ctggcttatt agcggctgca
      901 ggtcttcatc gaccggaaac tgatgggcat gaacctcccg caggtagccc aggagatcga
      961 acgccgccat gatggcgctc ctcccgacca gcgggtagag ggaactgtgg ccgggtgtgc
     1021 cgcgaaaaga gatatcaagc cggtagagac ccttctgccc gatcgccggg ctggtgtgcg
     1081 gtgtcggttc tgcgatcagg cactcacgcg gcttgagcag accctctgca agcagcgacc
     1141 ggatgccatg ctcacccccg gtctcctcgt cgcagacaaa ggcaaactgg acattcggct
     1201 ccacaccgct ctcgatgaga tcccggcagg caacaaggag tgcggcacaa ccgcccttca
     1261 tatccgtagc gcccctcccc cagacatatc catcggcaat cgtgccgctg aagggatcgt
     1321 agagccagtc atccggaatg gccggaacaa catctacgtg accgcagagg aggagttgtg
     1381 ggccgggttc ggtagtgacc aggttgtccc gcccatcagg gttggagacg atccggttct
     1441 tcaccccgag cgagtcaaga aactccgcga tgaacgcaac gatctcggtc gtgttccccg
     1501 gcgggttctc gctccggatc cggatcaacg atgaggcgag ccgggcgaca tccatgcagt
     1561 gctcccctta ctccgacctg gctgccgcat attcatcaaa gagcctcgcc agcgacgagc
     1621 ccttataaag ctgtttgata aacgccggat cgaagaactc atcgatgaac atcgagaggt
     1681 actcagatgg gatcggcccc ctctcatcgg ggttgagcac gatccggaaa ctcctgtact
     1741 gggcgggatc gtctgtatcg tatatacctg tccagagcat cgggagctct tcatagacct
     1801 caggatgctc ggtcttaagc cagttgatga accccgggaa atgtacccgg tcaccgacct
     1861 tctcctggtc cagtcgccac cgcatgtact ccatgctcat gatgttcctc ggcgacatgg
     1921 ggttgtaggc gagaacggtt gcagtgaaat cgatgtgagc gagggcatcg gtgaaataaa
     1981 agtgcagaac cggatgaaaa tcgctggggc tctccaggat cagcgatttg ttgtagagtt
     2041 tctccattac gcggacatac tcgttcgttt ccagcatacg ttcacccgtt ggctagcgtg
     2101 gttcaaataa tattcctttg atgcagcagg gcagcaacgg cattcagcgg cctggacgtg
     2161 gtatgtcgca cctatgtgaa atacggcctg cggcggctga ccgcctcacg aaaggcatct
     2221 tcaagttcct tgccgtgctt gccccggatg tttaccaggt tatccgtccg gaggaggcag
     2281 ggcttgaggt tgccatcaga ggttacccgc agcctgttac agaaggcgca gaactctgtg
     2341 ttatggagtg gcctgaccac ctcaacctcg gcgccgtcga ggcagtactt cttccggtgg
     2401 tgcatcctcc tggtgacgac ccgcgttgcc cgctcgttta attcctgctc gacgctgtcg
     2461 acatccccgt ggaacctgca ctcgttgaac tccatcagtt caataacctg caggaccagg
     2521 tcacgttttc cccggacaaa cgccataaag tcgtctatct catcctcatt gatcccgtca
     2581 agcaggacca tgttgatctt gaccggtgtc aacccggcct ggagcgccgc atcgatccct
     2641 tccagagcat cctccaggca gtcctttccg gtgatccggc tgtagcgctc gggtcggagc
     2701 gtatccagac tgacattcac ccgtgcaaga ccggcttcct tgagttccgc ggcctttgga
     2761 gcaagcagcg tcccgttagt cgtcatggac gactcgactc ccggcggcac cgagcggatg
     2821 atatcgacaa gatcccggcg tagcatgggt tccccgccgg tgaatttgac gctcctgatg
     2881 ccaaactgtg caccgatcct catcagttca gcgatctcgt ctgcgctcat ctgctccttc
     2941 gggttcacct cgccttcggc atgacagtag atacatcgca gattacaccg cgatgtaagg
     3001 ctgatccgga ggttggtcac ggttcgatta taggggtcct tcagcatcgt tgcatgctcc
     3061 tttgaaattc tttgcaatga tatagatctc agcactcccc ttccgcgatg cttttgtcct
     3121 gtaactccgg acggaataga aatgcttcct ggcttcggcc acaagctccc cgaagaactc
     3181 accctggaac gatttcaaaa ccatatttcc accgggcttc aagattgtgc aggcgaacgc
     3241 gagtgcgtcc tcaccaagcc cgatcgcacg cgcctgatca taactcttct ggccagagag
     3301 ttttggcgac gcgtctgata ccacaacgtt gaccgtgcca cctgcctctt gccggatacg
     3361 ttcctggaca ggaggatcgg tgaagtctcc gacgagggtg atgacacctt ccatgggtgc
     3421 aattgggttt aaatccacgc ctattatccg gccgtctgtc aggtcgcgaa gcacctgcag
     3481 ccagctcccc ggcgccgccc ccaggtcaac aacgttgtcg tcggccctga taacaccatt
     3541 gcgctgctgg atctccagca gtttgtaggc ggcccgcgcc ctgtagcctg actgcatcgc
     3601 cttgcgatag acgctatcct tcgtccactg cgaacccatt gataaactgc ccccacctga
     3661 ctctgactgt cggttgactg caccgcctgc attatcaagg agactatgta tagtatttga
     3721 tgcagttaac tatgaatgat gtggtctaat aatcacgaac atctccctca ccccgtgcga
     3781 tgaagtccac cacagatttt gatcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnaatca cgaacatctc cctcaccccg tgcgatgaag tccaccacag attttgatcg
     3961 taaaggacta ttatgagtga aggagactat gtactctcaa cgagtgttat aaaggggtca
     4021 caatgttaaa ggcaacgatt gatgcagaaa ttttccggga gtctatcgac gcgatcgccg
     4081 cgctggtgac agagtgccgg ttgcacacgc aagaggatat gatctggaca cgctctgtcg
     4141 ataccgcaaa cgtggccatg gtcaccctgg aactccagag cacagcgttc acatcgttct
     4201 cggcgacagg cggagaactg ggcctggatg tcgcgaagtt gaagaacatc ctggcgatga
     4261 taggtaaggg cgatgctctg acactccaac tgcaagacaa cgaccggaaa ctggaactca
     4321 gtttcggcgg ctaccgttac tctatcacac ttcttgatgt taacaccatc cggaaagacc
     4381 caaacccacc ggcagtcaac ctcccgggga aggccgtaat ccccggagag acgctgaaca
     4441 acgccatcaa ggctgcaagc gtcatatccg acaagatcgc gctcggcatc gaccctgaat
     4501 ccatgacttt ctacatggag gccgaggggg acaccgatca catcaaactc gcgctcagcg
     4561 aggacgaact catcgcctta aaccctgtca aggcccgctc cctcttctcg atcgattact
     4621 taaaggatat gggcagggtc atggcgcgcg cagagaaggt tgagatccac ctcggcatca
     4681 atcatcccgt gcggtttgtc tttgagattg cagacggtaa cggccgtgtc gattacctcc
     4741 ttgcccccag ggtcgaggca gattgatgga tatcgtactc gatcgcaaag aactcaccaa
     4801 atatccattt ttaaaagagt cgcagcagct ggccagccag cacgttgaat ccctcgaggc
     4861 gttcctcgcc agcagtgccg gaaacgtggc gcttgaacgt gcaaaggaac gcatcatcgc
     4921 tgcgctaagg ttcaggcgag atttccagga tgacgccgcg cggggcccgg cccccgaact
     4981 cgagatagcc agttatgcgc ttgcccgtat aattgtctcc tgtataggcg accgatcgct
     5041 tatcgaccgc cttgcacgct acgaatctga ccggtcatca ttcttcctgg cagccgaaga
     5101 acccgaggtg cgtcgtttcg ttgccctgcg gctccagatc gaccccgacg ccagaagcat
     5161 gccggtaact gactacgtgg aactggcagc ccgcctgcgt gataatcgct ggaggctcgt
     5221 caacagggat gtcaaccagg gagttgtggg tgtcgagccc ggcgagatcg acgaactgat
     5281 ccgggaacgg atacgaacga tcgtcgccga taacctcccg ctacgggtac ccggcgggat
     5341 atgcgaaacc ctggcggcgg tggccagcga gatcaccgca ctccagcagc agcaggcact
     5401 cgaagaattc ggggagatcc gggaggaggc gtttccgcca tgcattgcca gtctcctcca
     5461 ggcgctcaca gcaggaacga acctgacgca catgggacgg tttgccatca catcgttcct
     5521 tcacactatc ggtatgaaca tagcccagat agcggaaatt ttcgcgcagg cacccgactt
     5581 tgacccggat atgaccatgt accaggtgga gcacatctcc ggccgtggcg gcaccgagta
     5641 caccccgccg tcatgcatca cgatgcgcac cttcggcctc tgtgtcaacc gggataaaga
     5701 atgcgagcgg gtcagccatc ccttgagtta ctaccggctc aagaaaagcg gtgcaaaaaa
     5761 acaggtttaa
//
LOCUS       DAOSZX010000091         5730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11769_length_5730_cov_21.279824, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000091 DAOSZX010000000
VERSION     DAOSZX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_5730_cov_21.279824"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(698..1450)
                     /locus_tag="PK196_07065"
     CDS             complement(698..1450)
                     /locus_tag="PK196_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HON41212.1"
                     /translation="MDEEPVEILVGMAERGEVDPWNIDIVDLTDRFLAELERRKRLDL
                     RISGRTLFYAACLLRLKSEYLDARDDDVEEAFDDEEGESWGYGGFDFDDAAGDVEPVE
                     RLEREIQRRLGRKNLRKRLPVTLYELIKQLKTAEKEQRRRQRRRAPLATEPDLDLSAG
                     DVVSVAHDEGYQGAVAAVMEGFRCAARRSGGVVTLERLSSEMGESRRDVYIPLLFLML
                     EGKLEIWQDEFFGEVYVGERAAGDGPGDADRA"
     gene            complement(1443..4886)
                     /gene="smc"
                     /locus_tag="PK196_07070"
     CDS             complement(1443..4886)
                     /gene="smc"
                     /locus_tag="PK196_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HON41213.1"
                     /translation="MHITHLEIDNFKSFARKTKIPFFEGFTVISGPNGSGKSNIIDSI
                     LFVLALSGARGLRAEKLTDLINVNSGKNTAEVTITFSDGTTIRRRIKRTPTGYYSYNY
                     LNNRPCKQSDVIEFLARIGIKPEGYNVVMQGDVTRIMEMSDTERRKIIDEIAGVAEFD
                     QKREQAFSELEVVRERLDREEILLNEVAARLDTLQREREQALEYRRRQDELEHLNRCR
                     GAALVRQKEQEIATLHDLMRDHRAAIERTAAEIEAVRARAEEARTRQQGIDEEINRKS
                     GPEYLELVGRLEEARSAIRLGEKSIERLKASLEENAGAVQRVYMDSRRAEAKVQECTE
                     SLRSLSIDRSNLAMELAAKRDEMKGIEERIAAESREVEGVKDRLFARLQDLESRKELR
                     AQILREKDLLIEKSRMRTSERERLDARISQIEEELGNKQAQIADYSSSLAGCMEQKRR
                     VERDLSETESTLFGRRSALDGLKKEIRENEQALMRLEAQQQAHGDAGGKAMDYVLGMD
                     GVHGTVAHLGRAPPEYATALDVAAMGRLRWVVVDNDAVAAEAIRYLKENRLGRVTFLP
                     LNRLRPPVLQPLGAEPGIIGYAVDLLEFDPAFERAFQVVFGSTVVVDTLDRARRLMGR
                     YKMVTLDGETVERSGAMTGGFQAKKVRGFGVAVDDEVARIRARLAELEAEAGEIEASI
                     GRFALAAEAKRAERSSIEEQIARYRMLIEEFQKRVEVLAGEKHTLEEKRREVLEGAKT
                     GGEELARLEADLERTSAEIAQISAEVDGLKKKLDDTEVPALTERYENYRRTVEDIERR
                     LRNKDADITDIRRERQHYSNRIEELAAERSRLEEKNREIESEIKAAGEQIEENRRLIV
                     QLEARQKEFSNELAGLRETRDRILEEIRTLDQEVFNLSSEMERSRMQVEALEERERTL
                     FQELADLREQAGGVETDLDLAAIDAGIAAAERALKKIGAVNMLAIEECERVAARVEER
                     TQKKEVISREREMLLERIERCEKMKYDAFMTAFKAIDANFQEIFARLTDGSGRLVIEN
                     EEDPFSGGLTFAVQPRGKKVHLLSSLSGGEKSLTTLAFIFSIQKYMPAPFYALDEVDM
                     FLDGSNVGRIADMMSELSGNAQSIIVSLRKPMIERADRIVGVTTRADKSTYVTGVQDN
                     G"
     gene            complement(4901..5515)
                     /locus_tag="PK196_07075"
     CDS             complement(4901..5515)
                     /locus_tag="PK196_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41214.1"
                     /translation="MAQKDARIHFLERELAEREKEMQMMKEREQLPVAGADDERLREL
                     ERKVRELDALVKGLMEEVLDVKSVAMKLARETDERRERRPVQPAAAKPAQELRAEPRT
                     VEPRGETRPAGKRPSKPVPEEKTPDEGDFEMIMQNDGTLKPEPKRPSSEYIVASTGMK
                     DLPQKPVKKGGKSSDRKVFVEQRKRPVDDLIQAEEDDTVDLDHR"
BASE COUNT         1037 a         1993 c         1466 g         1234 t
ORIGIN      
        1 ccgctcgacc tccccaagtt cactgaaagc atccccgatc tcggccttaa gctctgtcac
       61 tggcggtgta aaattcccca ctttcggcgg gataaatttc cccacccgac aaccttcatg
      121 caaccgtgtg agggcagtgg caatgctgaa gatggaggaa cttctcgtga tacgagatct
      181 attcaatcaa ggaccgaaca aaagtcagat tgcccgaagt gtcaacggcg gcgtaacttt
      241 gtgcactttc ggcggtctaa aagtgtacac ctgctaaccc tctcaaggac gcagttgagg
      301 ggcgcagcaa ttgttcactg tggaggaatt cctcatgatc cgcgacttgc atcatcaggg
      361 gctgaacatc agccagatct ccagggagac gggatatcat cgaaatacag tgcgaaaata
      421 ccttgctgcc caaaccatgc caataccggc ttctcgaagg acacggccga gccaaactcg
      481 atccttataa tgagtatatc cagaatcgcc tttcagaata ccccttgagt ggagcaaggg
      541 ttcacctgag gctacacaat tctcaaggaa tacgtccgaa agatccgtcc tgctggagga
      601 gcaccggcac tacttcggta cgagaccaaa cccggggtcc acgcccgggt ggactgggga
      661 gaatgtgatc gcatcgaagc ggatggcagg acacggatca tgcccgatca gcatcccccg
      721 ggccgtcgcc agcggcccgc tccccgacat acacctcgcc gaagaactca tcctgccaga
      781 tctcaagttt cccttcgagc atcagaaaga gcagcgggat gtagacatcg cgccgcgact
      841 ctcccatctc gctcgaaagc cgctcaagcg tgacaacacc cccactccgg cgggcggcac
      901 accgaaaccc ctccatcacc gccgccacgg cgccctgata accctcatcg tgcgccaccg
      961 agacaacatc ccccgcgctg aggtcaagat ccggctccgt cgccagaggc gcccgtctcc
     1021 tctgccgccg gcgctgctcc ttctccgccg tcttcaactg cttgatcagc tcatacagcg
     1081 tgaccggaag acgttttcgc aggtttttcc gccccagacg gcgctggatc tcacgctcca
     1141 ggcgttcaac gggttcgaca tcccctgcag catcatcgaa atcgaacccg ccataccccc
     1201 acgactcacc ctcctcatca tcaaacgcct cctcaacatc gtcgtcccgg gcgtcgagat
     1261 actccgattt gaggcgcagc aggcacgctg cgtagaaaag cgtccttccc gagatcctca
     1321 gatcaagcct cttgcgacgc tcaagctcag cgaggaaccg gtcggtcaga tcaactatat
     1381 cgatgttcca ggggtcgacc tcgccgcgct cggccatacc aaccaggatc tcgacaggct
     1441 cctcatccat tatcctgcac accggtcacg taggtgctct tgtcggcgcg ggtcgtcacg
     1501 ccgacgatac ggtcggcgcg ctcgatcatc ggtttcctga gcgagacgat gatcgactgt
     1561 gcgttccccg agagttcgct catcatgtca gcgatccggc cgacgttgct gccgtcaagg
     1621 aacatatcca cctcatccag cgcgtagaac ggcgctggca tgtacttctg gattgagaag
     1681 atgaaagcca gcgttgttag cgacttctca cccccggata gagaagagag gaggtgaacc
     1741 ttcttcccgc gcggctgcac cgcaaacgtc agccccccgg agaatggatc ctcctcgttc
     1801 tcgataacca gcctcccgct cccgtcagtc aggcgcgcaa agatctcctg gaaattagca
     1861 tcgatcgcct taaacgccgt catgaaggcg tcgtatttca tcttttcgca cctctcgatc
     1921 cgctcaagga gcatctcgcg ctcccgcgag atgacctctt tcttttgtgt ccgctcctca
     1981 acgcgcgccg caacccgctc gcactcctcg atcgcgagca tgttgaccgc gccgatcttc
     2041 tttagtgcgc gttcagccgc cgcgatgccc gcatcgattg cggcaaggtc gagatcggtc
     2101 tcaacaccgc cggcctgttc cctgaggtca gcaagttcct ggaagagcgt gcgttcccgc
     2161 tcctcgagcg cctcaacctg catccggctg cgctccatct cgcttgagag gttgaagacc
     2221 tcctgatcga gtgtccggat ctcttcaagg atccgatcgc gcgtctcgcg cagccccgcg
     2281 agttcattgg agaactcctt ctgccgggcc tccagttgca cgatcagccg gcggttctcc
     2341 tctatctgct cgccggcggc ctttatctcg ctctcgatct cccggttttt ctcctccagg
     2401 cggctccgct ccgccgcaag ctcctcgata cggtttgagt agtgctggcg ctcgcgccgg
     2461 atatcggtta tatcggcatc cttgttccga agccggcgct cgatatcctc aacagtccgc
     2521 cggtagttct cgtaacgctc ggtgagcgcc ggaacctccg tatcgtcaag tttcttcttg
     2581 aggccgtcaa cctcagccga gatctgcgcg atctcggcgg atgtccgctc gagatcggcc
     2641 tccagccgcg caagttcttc gccgccggtc ttcgcgccct caagcacctc gcgccgcttc
     2701 tcctcgagcg tatgcttctc accggcaagc acctcgaccc gcttctggaa ctcctcgatg
     2761 agcatacggt atcgtgcaat ctgctcctcg atcgagctcc gctccgcccg ttttgcctcc
     2821 gcggccaggg caaatcgacc gatggacgcc tcgatctccc cggcctccgc ctccagttcg
     2881 gcaagcctcg cgcggatgcg tgcgacctca tcgtcgaccg ccacaccgaa cccgcgcacc
     2941 ttctttgcct ggaaaccccc ggtcatggcg ccgctcctct cgacagtctc accatccagc
     3001 gtgaccatct tgtagcggcc catcaacctg cgtgcgcggt caagggtatc caccacaacc
     3061 gtcgagccaa acacaacctg gaacgcgcgc tcaaacgccg ggtcaaactc caggagatcg
     3121 acggcatacc ctatgatccc gggctccgcc ccgagcggct ggagaaccgg cggccggagc
     3181 ctgttgagcg gcaggaaggt gacccggcca agacggttct ccttcaggta cctgatagcc
     3241 tcggccgcca ccgcgtcgtt gtccacaacg acccagcgga gtcggcccat cgccgccaca
     3301 tccagcgcgg tcgcgtactc cggtggagcg cgcccgaggt gcgccaccgt cccgtgaacg
     3361 ccgtccatgc caaggacgta gtccatcgcc ttccccccgg catcgccgtg agcctgctgc
     3421 tgcgcctcaa gccgcatgag ggcctgctcg ttctcccgga tctctttctt cagcccatca
     3481 agcgctgaac ggcgcccaaa tagagtgctc tccgtctcag agagatcgcg ctcgacccgc
     3541 cgtttctgct ccatgcaccc ggcaagactg gaggagtagt ccgcgatctg ggcctgtttg
     3601 ttcccaagtt cttcctcgat ctggctgatc cgggcgtcca gacgctcccg ctccgacgtc
     3661 cgcatccggc tcttctcgat gaggagatcc ttctcgcgga ggatctgcgc ccggagttcc
     3721 tttctgcttt cgaggtcctg caaccgcgca aagagccgat ccttcacccc ctccacctcc
     3781 cggctctcgg ccgctatacg ctcctcgatc cccttcatct catcgcgttt cgccgccagt
     3841 tccatcgcca ggttcgacct gtcgatcgag aggctgcgca ggctctcggt gcactcctgg
     3901 accttcgcct cggcgcgcct gctgtccatg tagacccgct ggaccgcccc ggcattctcc
     3961 tccaggctcg ccttcaaccg ctctatgctc ttctccccga gcctgatcgc gctccgcgcc
     4021 tcttcaagcc tcccgacaag ttcgagatat tcggggccgc tctttcggtt gatctcctcg
     4081 tcgatcccct gctgccgggt tcgcgcctcc tcggcccggg cccgcaccgc ctcaatctcc
     4141 gcggcggtgc gctcgatcgc cgcccggtga tcgcgcatca ggtcatggag ggtggcgatc
     4201 tcctgctcct tctgccggac gagcgccgcc ccccggcagc ggttgaggtg ctcgagttcg
     4261 tcctgccgcc gccggtactc aagcgcctgc tcgcgctcgc gctgaagcgt atccagccgc
     4321 gctgcaacct cgttcaggag gatctcttcg cgatcgagcc gctcacgcac cacctcaagt
     4381 tccgagaacg cctgctcccg cttctgatcg aactcggcga cacccgctat ctcatcgatg
     4441 atcttccgcc gctcggtgtc gctcatctcc atgatacggg tgacgtcgcc ctgcatcacg
     4501 acgttgtagc cctccggctt tatcccgatc cttgccagga actcgatgac gtcggactgc
     4561 ttgcagggac ggttgttgag gtagttgtaa ctgtagtagc ccgtcggcgt ccgctttatc
     4621 cggcggcgga tggtcgtgcc gtctgagaag gtgatcgtca cctcggcggt gttcttcccg
     4681 gagttgacgt tgatgagatc ggtcagcttc tccgcccgca gcccccgcgc gcccgataga
     4741 gcaaggacaa agagtatgct atctatgatg ttgctcttac cggagccgtt cgggcccgat
     4801 atgactgtaa atccctcaaa aaagggaatt ttagtctttc tggcgaaaga cttgaagttg
     4861 tcgatctcaa ggtgcgttat gtgcaagaat tgggcccctt tcacctgtga tccaggtcaa
     4921 ccgtatcatc ctcttcggcc tggatgagat catcgaccgg gcgcttcctc tgctcaacaa
     4981 agaccttccg gtcagacgac ttgccgccct tcttcacagg cttttgcggc aggtccttca
     5041 tcccggtgct ggcaacgatg tactccgatg aagggcgttt tggctccggt ttcagtgtcc
     5101 cgtcgttctg catgatcatc tcaaagtccc cctcatcggg agttttttcc tccggaaccg
     5161 gctttgacgg ccgcttcccc gccggacgcg tctcgccccg gggttcaacg gtgcgcggct
     5221 ccgctctgag ctcctgcgcg ggctttgccg cggcaggctg cacgggcctc ctctcccggc
     5281 gttcatccgt ctcccttgca agtttcatcg ccacggactt gacgtccagg acctcttcca
     5341 tcaggccttt caccagtgca tcgagctcgc gcactttccg ctccagttcg cgcaggcgtt
     5401 catcatccgc ccccgccacc ggcaactgct cccgttcctt catcatctgc atctccttct
     5461 cgcgctccgc aagttcccgc tcaagaaaat gtatccttgc atccttctgc gccattatct
     5521 ccctcaaact aatccttcat aataattatg taataatata actatggatt cctgaattga
     5581 agaattatct cgcggcttga agccagcatc tccaggaagc cccatgggcc ggcgtcaggt
     5641 cgaaaccgat gccatgtcgt gccgcaagac aaaaagaggc cgtcaaatca acggttctgt
     5701 gatataaact ccccgtttct tcccggtatt
//
LOCUS       DAOSZX010000092         5700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_11874_length_5700_cov_12.242693, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000092 DAOSZX010000000
VERSION     DAOSZX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11874_length_5700_cov_12.242693"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..3873
                     /locus_tag="PK196_07080"
     CDS             181..3873
                     /locus_tag="PK196_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HON41215.1"
                     /translation="MFHHDQQRTGYVPEEGPQTNAILWIAETAEWADGSPAVYNGKVF
                     VPTWPDMNFGEASPMGLVCYDAVTGAEAWTNELGGASVGSVSGVAVADGCVYLGGTDG
                     RLYCIDEETGETLWVSDRIDTTGYFGLSSSPLVYEGKIYALSASDGVLHAFNAGGTEA
                     WRFETGNGVMYFTSPAGYDGKIYCAGNRSQIFCIDPAAQTAIWTFNQGGSVKSSPVIG
                     ADGTVYFTTSSRCYALDGSTGKERWNSSVTGTMSTPVIADGYIYAGAYDGLHCLDAST
                     GEEEWHFQAKEVNVAPVVAGNLVYAATNEETGTLYAVDAETGEPVWSYSLEAPGDGTF
                     SAFYTSSPAVSGGVLYIGAENNRFYAFGEGEVPEPAFTGWSGTVSLADGQTFEVTPFN
                     NESAVYSINRTSAFGALDAAAMKGGFNYTIQETAWGLFLYSIGGIAYNETTWDSWLYS
                     VNGVPAEVGAADYSLREGDVVTYWYGPWGSSPETARAAVNITVSIPLPPASVTTLWNG
                     TVALIEGETFQFVPSNNESASYTINRTTDLGALDAAAVSGNFTFNASDTWYATYGSFL
                     LEDIAGIANEDWTEENARSWSIFINGAAASAGLGANILADGDTLAFYYCPADPDTYAP
                     LIDQAEYAVIIDVTLRGFAWGGSVSLTDGQTFEVTPFNNESAVYTINRTSALGALDAA
                     ATKGGFNYTIQETAWGPFLYSIGGIAYNETTWDSWLYSVNGVPAEVGAADYSLREGDV
                     VTYWYGPWGSSPETARAAVNITVSIQASGGGSGGDGGGGGDSSPSWVSVTLAAGTFNI
                     TATNSGKTYTVDRQTALGALDATGIAYTINDAYYQEYGSLFIDSIRGRANEGTAGWMY
                     QVNAESPGVGANAYAVQNGDEVVFFWSEGMSSTPATSRDTIFIRVVTPGSSSGGSGSG
                     SSGSSSGGSGSTSTTATTGTGEPGEVLSFPLGLPAGATVKLGEWGQSFSIDLTAGDRS
                     TEQVNVSGNTVTINREGVRLVITARNIDKKGGLAAGLVERVTASVGPVRANFSTTGVI
                     AASVNLNLTGIPGDGQIRISLNETPDAETAREFHLASAENGKDVTAVAYSMTVTRINL
                     ENGRDVAGAVITMAINPAWVDEHGGPDAIRIARSAEDGTCTILDTRPAGSDADGNLIF
                     EAVSPNGLSTFGLLAVGVVPAAGAGGEEETGVIAPTTAPGSLATTPEMHNTAANLPLS
                     SPLLIIGVCTILLVGAAYLIIKGRRT"
     gene            3873..>5700
                     /locus_tag="PK196_07085"
     CDS             3873..>5700
                     /locus_tag="PK196_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HON41216.1"
                     /translation="MKRIGLTLFILLLCVVSCGAYPLNTDDPGIQASVDYIRGCQKSD
                     GGFGEIGRESSPGTTSWVIMAAVAAGEDPRNWANGGNSTLDYLRSMNDEILAKGGTVD
                     IARTILTLVAIGEDPRAFCGTDHVTELKSRVKPDGQAGDHVYTTIWTVIALASVGEDT
                     DSSAEWLIARQNTDGGFPWTPGAESDPDDTGAALEALAAAGVSSDATVVQNALAYLKG
                     MQQEDGGFHYGGTSATNAASDAWVIQGIVAAGENPAEWVVGERGVVEHLLGLQNPDGS
                     FRYTAYVTDSPCRMTASAIPALLGMPYPITSAQVQQPQSSTPEPRATTVPLATTPTAT
                     PTVATASVVTDNSQRSVTVTDDFGEIITIRGTPQRIVSLAPSNTEILYALDLDDRVVG
                     VTDYCNYPPEAADKPRVGGYSTVNIEKVIAAEPDLVLAALGNTEDVVNRLRSLGLTVV
                     SLNPLTVEDVLHDIELLGVVTGQEEEASTLVGELRARVEAVTGKTETLEEKPSVTHVV
                     WYDPIWVSGRDTFQDEVIRMAGGVNVFDQVDGWGIVSLEEFIVMDPDYILVSSGDGMS
                     ERGRDIVYDYFMNEPRMQELTAVRNGRVYVIDADAASRGGPRI"
BASE COUNT         1198 a         1683 c         1724 g         1095 t
ORIGIN      
        1 gccaggttta ggtttggtta cacaagcaaa ttatatgtag ttacataaaa ttaaatttgt
       61 aaagttttat taatttagat aaagtgtact tgtactatga gacctaaacg gttgctcccc
      121 tttgcgggat ctattatctt tttgatactg ctcgttggag tggcgagcgc gtcatatcca
      181 atgttccacc acgatcagca gcggaccggc tacgttcccg aagaggggcc gcagacgaac
      241 gccatcctct ggattgcgga gaccgctgaa tgggctgatg gatcgcctgc ggtgtataac
      301 ggaaaagtct tcgtccccac atggcctgat atgaacttcg gcgaagccag cccgatgggg
      361 cttgtctgct atgacgccgt caccggggcg gaggcatgga cgaatgaact cggcggcgcc
      421 agcgtcggtt ctgtctcggg ggtcgccgtc gctgacggtt gtgtctacct cggcgggact
      481 gatgggcgac tctactgcat cgatgaggag acgggggaga cactctgggt gagcgatcgg
      541 atcgacacca ccggctattt tgggctctcg tcatcgcctc tcgtctacga aggaaagatt
      601 tacgctctct ccgcttcaga tggcgtgctg cacgccttca acgctggtgg taccgaagcc
      661 tggagatttg aaacgggtaa cggtgtgatg tacttcacct ctcctgcggg ctacgatgga
      721 aagatttact gcgcaggaaa ccggagccag atcttctgca tcgatcctgc agcgcagacc
      781 gccatctgga cattcaatca ggggggatcg gtaaagtctt cgccggtcat cggagcggac
      841 ggaacagttt acttcaccac ctcgtcgcgg tgctacgccc tcgacggcag cacaggcaag
      901 gagcgctgga acagttccgt gaccgggacg atgtcgaccc ctgtcattgc tgatggttat
      961 atctatgcag gggcttatga tggcctccac tgcctggacg cctcgaccgg tgaggaggaa
     1021 tggcatttcc aggcaaaaga ggtaaacgtt gcaccggttg ttgccggcaa cctcgtctac
     1081 gccgcgacaa acgaggagac aggaaccctg tatgcggtgg atgccgagac cggtgaaccg
     1141 gtctggtcat actccctcga agccccgggc gacgggacgt tctctgcgtt ctacacatca
     1201 tctcccgctg tatcgggtgg tgttctctac atcggagccg agaacaaccg cttctacgcc
     1261 ttcggggaag gagaggtacc ggaacctgcg tttaccggat ggagcggaac agtcagcctg
     1321 gcagacggcc agaccttcga ggtcacgccg ttcaacaacg agagtgccgt ctacagcatc
     1381 aaccggacat cagcgttcgg ggctctcgat gccgccgcaa tgaaaggcgg gtttaactac
     1441 acaatccagg agaccgcctg gggtcttttc ctctactcca tcggcggtat cgcctacaac
     1501 gagactacat gggactcctg gctctattcg gtcaacgggg tgcctgcaga ggtcggtgcc
     1561 gccgattact ctctcaggga gggggacgtc gtcacatact ggtatggtcc ctggggttca
     1621 tcgccggaaa ccgcccgtgc ggcggtcaac atcaccgtct ccatcccgct gcctcctgca
     1681 tcggtcacca cgctatggaa cgggaccgtg gccttgatag aaggagagac cttccagttc
     1741 gtcccctcga acaacgaaag tgcctcatac acgattaacc gcaccactga cctcggggct
     1801 ctcgatgccg ccgcagttag cgggaacttc accttcaacg cctccgacac ctggtacgcc
     1861 acctacggtt cgttcctgct cgaggatatc gccggcatcg caaacgagga ctggacagag
     1921 gagaacgcac gctcatggtc gatcttcatc aacggtgcgg cggcatccgc agggctcgga
     1981 gcgaacatcc tcgcagatgg cgacacgctc gccttctact actgccccgc ggatccagac
     2041 acgtatgccc cgctcataga ccaggccgag tacgccgtca ttatcgacgt caccctccgc
     2101 ggctttgcgt gggggggatc ggtcagcctg acagacggcc agaccttcga ggtcacgccg
     2161 ttcaacaacg agagtgccgt ctacaccatc aaccggacat cagcgctcgg ggctctcgat
     2221 gccgccgcaa cgaaaggcgg gttcaattac acaatccagg agaccgcctg gggtccgttc
     2281 ctctattcca tcggcggtat cgcctacaac gagactacat gggactcctg gctctattcg
     2341 gtcaacgggg tgcctgcaga ggtcggtgcc gccgattact ctctcaggga gggggacgtc
     2401 gtcacatact ggtatggtcc ctggggttca tcgccggaaa ccgcccgtgc ggcagtcaac
     2461 attaccgtct ccatccaggc atcgggtggc ggcagcggcg gagatggcgg tggcggcggc
     2521 gattcgtcac cctcctgggt cagcgtcacc cttgcagcgg gcacattcaa catcaccgct
     2581 acgaacagtg gaaagaccta cacggtcgat cgacagacgg cgctgggggc cctcgatgca
     2641 acggggatcg catacaccat caacgacgct tattaccagg agtatggctc gctcttcatc
     2701 gactccatcc ggggcagggc gaacgaaggg accgcgggct ggatgtacca ggtgaacgct
     2761 gaaagccctg gcgtcggtgc aaacgcatat gccgtccaga acggcgatga ggttgtcttc
     2821 ttctggagcg agggtatgag ttcaaccccg gcaacctcgc gggataccat cttcatcagg
     2881 gtcgtgaccc cggggtcgtc gtccggtggt tccggatcgg gttcatcagg aagcagttcc
     2941 ggcggttcgg gttccacatc gaccacggcg accacgggaa ccggtgaacc aggagaggtc
     3001 ctctcgttcc ccctcgggtt accggcaggg gccacagtta aactcggaga atggggtcag
     3061 agtttctcga ttgatctcac cgcaggagat agatccacgg aacaggtaaa cgtatcagga
     3121 aacaccgtca ccatcaaccg ggaaggtgta agactggtca tcaccgcccg gaacatcgat
     3181 aagaaaggcg gcctggccgc gggcctggta gagcgtgtca cggcatcggt cggccccgtc
     3241 agggccaatt tcagcactac gggtgttatt gccgcatcag taaacctgaa cctcacaggc
     3301 atccccgggg acggccagat caggatctca ctgaatgaga cccccgatgc agagacagcg
     3361 cgcgaattcc atctcgcctc ggcagagaac ggcaaagatg tcaccgcagt tgcctacagc
     3421 atgaccgtta cgcggatcaa cctggagaac ggcagagatg tcgcgggagc ggtgatcacg
     3481 atggccataa accctgcgtg ggtggatgag cacggcggtc cggatgccat aaggatcgcg
     3541 cggtctgcgg aggacggtac ctgcaccata cttgataccc ggccagccgg gagcgatgca
     3601 gatggcaacc tgatcttcga agcagtatct cccaacggtc tatctacttt cggccttctg
     3661 gcggttgggg tggtcccggc cgccggggcc ggaggagagg aggagaccgg ggtgatcgct
     3721 ccgaccaccg ccccgggcag tcttgccacc acaccagaga tgcacaacac agcggcaaac
     3781 ctgcccctct catcaccgct tctcattatc ggtgtgtgca cgatcctgct cgtgggagcg
     3841 gcatacctca tcatcaaggg gaggagaacc tgatgaagcg gatcggtctc accctcttca
     3901 tcctcctcct ctgtgtcgtc tcgtgcggcg cttatccgct gaacacggat gaccccggca
     3961 tccaggcctc ggttgattat atccgtggtt gccagaagag tgacggcggt ttcggggaga
     4021 tcgggcgcga gagcagccca ggcacgacat cgtgggtgat aatggcggcc gtcgccgctg
     4081 gcgaagatcc gcggaactgg gcgaacggag gaaactcgac ccttgactac ctccgctcga
     4141 tgaacgacga gatcctggca aaaggaggaa ccgtagatat cgcccgcacc atcctgactc
     4201 tggtcgcgat cggagaagat ccccgcgctt tttgcgggac tgaccacgtc acggaactga
     4261 aatcccgcgt aaaacctgat ggccaggcag gagatcacgt ttacacgacc atctggacgg
     4321 tcatagccct ggcttcggtc ggcgaagata ccgattcgtc ggccgaatgg ctgatagcca
     4381 ggcagaacac cgacggtgga tttccctgga cgccgggtgc tgaaagcgac ccggacgata
     4441 ccggtgccgc gctggaagcc ctcgcagcgg caggcgtctc cagcgatgcg actgtggtgc
     4501 agaacgccct cgcctacctg aaaggaatgc agcaggagga cggcgggttc cattacggcg
     4561 ggacgagtgc tacaaacgct gcctccgatg cctgggttat ccagggtatc gtcgccgcag
     4621 gggagaaccc tgccgagtgg gtggtggggg agaggggtgt ggttgagcac ctcctcgggc
     4681 tccagaaccc cgacggatcg tttcgctaca ccgcttacgt gaccgacagt ccctgccgga
     4741 tgacggcaag cgcgatacct gcactccttg gaatgcccta ccccatcaca tctgcacagg
     4801 ttcagcagcc gcaatcctcc accccggaac cgcgcgcaac gactgttccc ctggccacga
     4861 ccccaaccgc gaccccaacc gtggcaaccg cttcggtggt gacggataac agccagagat
     4921 ccgttacagt gaccgatgac ttcggggaga tcatcaccat ccggggaacc ccgcagagga
     4981 tcgtatcgct tgccccatca aacaccgaga tcctgtatgc cctcgatctg gatgaccggg
     5041 tggtcggcgt caccgattac tgcaactacc ctcccgaagc ggccgataaa ccccgggtcg
     5101 gcggctacag caccgtcaac atcgagaagg tgatcgcagc ggaaccggat ctggtcctcg
     5161 ccgctctcgg gaatacagag gatgttgtca accgtctgcg gtctctgggg ctaactgttg
     5221 tctccctcaa cccgctgacg gtcgaagacg tcctgcacga catcgaactt ctcggtgtgg
     5281 tgaccggcca ggaggaagag gcctcgacgc tcgttgggga actccgggcc cgtgtagagg
     5341 cggtgaccgg gaagactgag acactggagg aaaaaccatc ggtcacccat gtcgtctggt
     5401 acgacccgat ctgggtcagc ggccgggata cgttccagga cgaggtgatc aggatggccg
     5461 ggggtgttaa cgtattcgat caggtcgatg gatggggtat cgtcagcctt gaagagttta
     5521 tcgtcatgga tccggattac atcctggtca gttccggaga cggtatgagt gagagggggc
     5581 gcgatatcgt ctatgactac ttcatgaatg aaccacggat gcaggaactc accgcagtca
     5641 ggaacggccg cgtctatgtc atcgatgctg acgccgccag tcgtgggggg ccgcggatcg
//
LOCUS       DAOSZX010000093         5436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_12620_length_5436_cov_17.986991, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000093 DAOSZX010000000
VERSION     DAOSZX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12620_length_5436_cov_17.986991"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..151)
                     /locus_tag="PK196_07090"
     tRNA            complement(65..151)
                     /locus_tag="PK196_07090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Ser,seq:gga)
     gene            complement(193..1104)
                     /locus_tag="PK196_07095"
     CDS             complement(193..1104)
                     /locus_tag="PK196_07095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HON41217.1"
                     /translation="MAGQESSSIPESGIVIIDKPRGPSSHQVTAWVGEILGRRVGHAG
                     TLDPQVSGVLIVMFGPAVRLAPVLLSHNKEYICLMRLHADASREAVERAAAEFVGRVY
                     QRPPRKSAVKRSLRIRKIHEIEILEMEGRLVLFRVRCDAGTYIRTLCVHLGYAIGTCA
                     HMVELRRTRSGRFSETDAVTLHELADAAVFAREGDPEPLQRMIHPPESAVADLPKVII
                     RDTAVDAVCRGAVLAGVGVREKAGGFAEGETVAIMTERGELVGLGRSLLGSSALRPGE
                     PGFAVAPTTVLMPPGTYPRGWKTHAGR"
     gene            complement(1111..1791)
                     /locus_tag="PK196_07100"
     CDS             complement(1111..1791)
                     /locus_tag="PK196_07100"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HON41218.1"
                     /translation="MKIAITRLENKAAGDRALCAAYGHDCYTVSPLRAELREERIDAF
                     IEAVHRGEFDCLFFASALPAALIAPRLERWPRVIAIGPKTAEALRASGIDAEVLPSFY
                     SRAFAPYLGDWLRGRTVGIPRADLPNPELIEAIVAAGGRVREERVYALVPTGTELEVE
                     DADALLFTSAMSYREARWQPRDGLLLIAIGTTTADAMRAGGHPPAVVGDGSLAGTLEA
                     LNRCVEGR"
     gene            1884..3095
                     /locus_tag="PK196_07105"
     CDS             1884..3095
                     /locus_tag="PK196_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HON41219.1"
                     /translation="MISSILLGTVLGILCGAVSGLVPGIHANTMAGLLLSLQALLLAW
                     FDPVVIASAMFATLVTHTFLDNVPATFLGIPDAETSLAVLPAHALCLDGRGEEAVRIS
                     ALGSAAGVALSLPLALGFVLVLPALQPAIDWGIGLIILAVGGYLVVASESPGWAFAVF
                     AVSGILGVFALDCSYLAWPAGGESGVLMPLLSGLFGVSVLLEGSQGKMPEQQFQGLDI
                     PPRALRRGSILGSAAGALVGWLPGLSSATANALLTSVVGYDSNPREYILATSAANTVD
                     AFLGLAAFYAISRTRNGVMAAISALEDMPPATAILLAGALAAVGAYLLTILFSRAAGL
                     FSGVNVTALNRAVIIFIVLLSFVLCGPFGILVLLLATAVGCVPALVNIRRVYCMGAIM
                     LPVMVYSFGLA"
     gene            3131..3994
                     /locus_tag="PK196_07110"
     CDS             3131..3994
                     /locus_tag="PK196_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HON41220.1"
                     /translation="MLQRYWFGDIDEDGCCRAAGTDPAALAERVSSLSTDMESYTPIN
                     WEVARDCGVVRTREEYVALLRSVCMILARQKIARSYQERDVALLQMVRMLDEVDNVIN
                     LLSERAAEWYQVTDPSFSRKYRSLPAKKMLDMIRRGADGGLAEVAAEIERLTEVRRRL
                     MREVSAMADEVMPNVSALIGGLVAARLLSRAGGLPALARMPGSTIQVLGSEQALFSHL
                     RGGSPPPKHGIIFQHRRVHNAPKEVRGRVARVLAAKLAIAARLDHYRGEAVPSFIEEA
                     QARIDEAGRRA"
     gene            3991..4590
                     /locus_tag="PK196_07115"
     CDS             3991..4590
                     /locus_tag="PK196_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HON41221.1"
                     /translation="MIWIGNVLVSPGEAGVYGERTLDGYRVWDPYRSKLAALYALGGG
                     VELEPWMRVLYLGAANGTTVSHVADYVETVYAVEFAPRPMQDLLEVARRRKKIVPIMA
                     DASRPEGYAPLMESVDLVYQDVAQPDQVEIAKRNLVFLKPGGWLILMLKTRSVDVRRD
                     PAGVLEETLAALDSRLEVVEARWLEPYHHDHAAIVCSRP"
     gene            4622..5356
                     /locus_tag="PK196_07120"
     CDS             4622..5356
                     /locus_tag="PK196_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HON41222.1"
                     /translation="MDRVVFNPFSPLLIIIFLGLIGLFIFAVLIFPLIFVTAVGATFT
                     RLGFSWQQALLILFLTLAGSFINIPIKTLESRPAAPEYDRYISIYGRLYHISRPVQRT
                     VLAVNVGGALIPIVISLYLLYKSVQIAGGFEILWLALLGVAIVTVVTKLVARPVPGLG
                     IATPFFIPPLAALLAALILPLLAGGVPGSSVIIAYVSGTLGTLIGADIMNLNHIAELG
                     APMASIGGAGTFDGIFLTGVIAALLA"
BASE COUNT          860 a         1727 c         1808 g         1041 t
ORIGIN      
        1 tgatgagggg gtcagaaagg cggtattcac ctattggggt gaagaattga acttgatttc
       61 caaacgctga gggggagatt tgaactcccg aggggctcaa cgccccacgg gctttccagg
      121 cccgcgcctt accggtctag actacctcag cagagatggt gcatcactcg ttggcggtgt
      181 attctattaa tattacctcc cggcatgcgt cttccagccg cggggatagg tgccgggcgg
      241 cataaggacg gttgtgggcg caacggcaaa cccgggttct ccgggcctca gcgccgacga
      301 tccgagaaga gagcgcccga gaccgacgag ttcgccgcgc tctgtcatga tggcaaccgt
      361 ctcgccctct gcaaaccctc cagccttctc cctgacaccg accccggcca ggacggcgcc
      421 gcggcagacg gcgtcgaccg cggtgtcgcg gatgataacc ttcgggaggt cggcaacggc
      481 cgattccggg ggatggatca tcctctggag gggttcgggg tcgccctccc gtgcaaacac
      541 agcggcgtcg gcaagttcgt ggagcgtgac ggcatcggtc tcgctgaacc gccctgaacg
      601 tgtccgccgg agttccacca tgtgggcgca cgtcccgatc gcgtagccca ggtgcacgca
      661 gagcgtccgg atgtatgtcc cggcgtcgca acggacccgg aagaggacaa gcctcccctc
      721 catctccagg atctctatct catggatctt cctgatccgc aggctccgct tcacagcact
      781 cttccggggc ggccgctggt acacccggcc gacgaactcc gctgccgcac gctctaccgc
      841 ctccctcgag gcgtcggcgt ggagcctcat cagacatatg tactccttat tgtgcgagag
      901 aaggacgggg gcaagcctga ccgcagggcc gaacatgact atgagcacac ccgagacctg
      961 ggggtcgagc gtcccggcgt gacccacccg gcgtcccagg atctcaccga cccaggcggt
     1021 cacctgatgg ctgctcgggc cgcggggttt gtctatgatg acgatcccgg attccgggat
     1081 ggaagaagac tcctggccgg ccatacgcga tcaccgccct tcgacgcagc ggttcagggc
     1141 ttcaagcgtc cctgcgagcg atccgtcccc gacgaccgca ggagggtgtc cgcctgcccg
     1201 cattgcatcc gcggtggtcg tgccgatggc gatgaggaga aggccgtcac ggggctgcca
     1261 ccgcgcctcc cggtaagaca tggcgcttgt aaagaggagg gcgtcggcat cctcaacctc
     1321 gagttcggtt cccgtgggga cgagggcgta gacccgctcc tcccgaaccc tcccgccggc
     1381 ggcgacgatc gcctcaatga gctccgggtt cgggagatcg gcccgcggga tcccgaccgt
     1441 ccggccgcgg agccagtcgc ccaggtaggg ggcaaacgcc cgggagtaga acgaggggag
     1501 aacctctgca tctatcccgg aggcgcggag ggcctcggcg gtctttgggc cgatcgcgat
     1561 caccctgggc caccgttcca gcctcggcgc gatcagcgcg gccgggaggg cgcttgcgaa
     1621 gaagaggcag tcaaactcgc cgcggtggac ggcctctatg aaggcatcga tcctctcctc
     1681 gcgcaactca gcccgcagcg gggagacggt gtagcagtcg tgaccgtatg cggcgcagag
     1741 ggcgcggtcg cccgcggcct tgttctcgag ccgggtgatg gcgatcttca ttggctacat
     1801 ccttcgtccc ggtggaatat gactgtatcg ggaaggacaa aatccggttg gtttatccct
     1861 gcccaatccg accatggata agcgtgatct ccagtatcct gctcggaaca gtccttggca
     1921 tactctgcgg cgccgtgagc ggtcttgtcc ccggcatcca tgcaaacacg atggcgggtc
     1981 tcctcctctc gctccaggcg ctcctgcttg cctggttcga ccccgtggtc atcgcctccg
     2041 ccatgtttgc cacgctcgtc acgcatactt tcctcgacaa cgtccccgcc acgttcctcg
     2101 gtatcccgga tgccgagacg tcgcttgccg tcctcccggc ccatgcactc tgtctcgatg
     2161 ggcgggggga ggaggccgtg aggatatccg ccctggggag cgctgcgggg gttgctctct
     2221 cccttccgct ggcgctgggg tttgtcctcg tccttcccgc gctccagccg gcgatcgact
     2281 gggggatcgg gctcatcata ctggcggtgg ggggctacct cgtcgtggcc tcggaatcgc
     2341 cgggctgggc gttcgcggtc tttgcggtct cggggatcct gggggtcttt gcgctggact
     2401 gctcctacct ggcctggccg gcgggcggcg agtcgggggt gctgatgccc ctcctctcgg
     2461 gcctcttcgg ggtatccgtc ctccttgagg ggtcgcaggg gaagatgccg gagcagcagt
     2521 tccaggggct tgacattccg cctcgcgctc tacgccgcgg gtctatactt ggttcggccg
     2581 ccggcgctct cgtcggctgg cttccggggc tctcgagcgc caccgcaaac gccctcctga
     2641 cctcggtcgt cggctacgac tcgaacccgc gggagtacat cctggcgacg agcgctgcaa
     2701 acaccgtgga cgcttttctc gggcttgcgg cgttctacgc catatcccgg acacggaacg
     2761 gggttatggc ggctatctcg gcgcttgagg atatgccgcc ggcaaccgcc atcctccttg
     2821 cgggggcgct cgccgcggtc ggggcatacc tcctgacgat cctcttctcg cgtgccgccg
     2881 gactcttctc cggcgtgaac gtcacggcgc tgaaccgcgc tgtcatcatc ttcatcgtcc
     2941 tgctctcgtt cgtcctctgc gggccgttcg ggatccttgt cctcctgctg gcgacagcgg
     3001 tcggctgcgt cccggcgctt gtcaacatcc gccgggtcta ctgcatgggt gcgataatgc
     3061 tcccggtgat ggtctactcc ttcggtctgg cgtgagcctc ttatacgaca ggggcgcaag
     3121 tattcctgca atgctgcagc gctactggtt cggggatatc gatgaggatg ggtgctgccg
     3181 ggctgcaggc accgatccgg ccgcactcgc cgagcgggtc tccagcctca gcaccgatat
     3241 ggagtcgtat accccgatca actgggaggt tgccagggac tgcggggttg tccggacgcg
     3301 ggaggagtat gttgccctgc tgcgttcggt ctgcatgatt cttgcccggc agaagatcgc
     3361 ccggtcatac caggagcggg atgtcgccct cctccagatg gtgcggatgc tcgatgaggt
     3421 cgataatgtc atcaacctcc tctcggagcg tgccgctgag tggtaccagg tcaccgaccc
     3481 gtcgttctcc cggaagtacc gctccctccc ggcaaaaaag atgcttgata tgatccggag
     3541 aggggcagac ggggggctcg cggaggttgc agccgagatc gagcggctca cggaggtgag
     3601 aagacggctg atgcgggagg tctccgcaat ggccgatgag gtgatgccga atgtgagcgc
     3661 cctcatcggc gggctggtcg cggcccgtct cctctcccgg gcaggggggc ttcccgcact
     3721 tgccaggatg ccgggaagca ccatacaggt gctcggctcg gagcaggcgc tcttttcgca
     3781 cctccggggc gggtcgccgc cgccaaaaca cggtataatc tttcagcacc gcagggttca
     3841 caacgcccca aaagaggtga gggggcgggt ggcccgggtg cttgcggcga aactcgccat
     3901 cgcggccagg ctcgaccact accggggtga ggcggtgcca tcgttcatcg aggaggcgca
     3961 ggcccggatc gacgaggcag ggaggagggc atgatctgga tcgggaacgt cctggtctcg
     4021 cccggcgagg ccggggtgta tggtgagcgg acgctcgatg gctacagggt ctgggatcca
     4081 taccggagca aacttgcagc gctctacgct ctcggcggcg gggttgaact cgaaccctgg
     4141 atgcgggtgc tctacctggg ggcggcgaac gggaccaccg tctcccacgt cgccgactac
     4201 gtcgagacgg tctacgccgt ggagtttgca ccccggccga tgcaggacct cctggaggtt
     4261 gcccgccgcc ggaagaagat agtgccgata atggctgatg cgagccggcc ggagggctac
     4321 gcgccgttga tggagtcggt cgacctggtc taccaggacg tggcgcagcc cgaccaggtc
     4381 gagatagcga aacggaacct ggtattctta aaaccggggg ggtggctcat cctgatgctg
     4441 aagacacgga gcgtggatgt tcgaagggac ccggccgggg tgctcgagga gactctggcc
     4501 gcgctcgata gccggcttga ggtcgtggag gcccgctggc tcgagcccta ccaccacgac
     4561 cacgccgcga tcgtctgctc ccggccgtag catatatcag cctggagggt gagagattca
     4621 catggaccgg gtcgtattca accccttctc gccgctgctg atcataatat tcctcgggct
     4681 gatcgggctc ttcatatttg cagttctcat cttcccgcta atcttcgtga cggcggtcgg
     4741 ggcgacgttc acgcggctcg ggttctcctg gcagcaggcg ctcctcatcc tcttcctgac
     4801 gcttgcgggg agtttcatca acataccgat aaagacgctc gagagccggc cggccgcacc
     4861 ggagtacgac cgctacatat cgatctacgg ccggctctac cacatctcgc ggccggtgca
     4921 gcggacggtc ctggcggtga acgtcggcgg cgcccttatc ccgatcgtga tatcgctcta
     4981 cctgctctat aagtcggtcc agatcgccgg cgggttcgag atcctctggc tcgctctcct
     5041 cggggtcgcg atcgtgacgg tcgtgacaaa acttgttgcc cggccggtgc cggggctcgg
     5101 gatcgcgacg ccgttcttca tcccgccgct cgcggcgctg cttgcggcgc tgatcctccc
     5161 gctcctcgcg gggggcgtgc cggggtcgtc tgtgataatc gcctacgtga gcgggacgct
     5221 cggcaccctt atcggggcgg acattatgaa tctcaatcat atagcggaac tcggggcacc
     5281 gatggcaagc atcgggggtg cggggacgtt tgatgggata ttcctgacag gggtaatcgc
     5341 ggcgctcctg gcgtaaaggg gtagggcgtc gggaggaatc cggcggcctg acccgggttg
     5401 cggcgaagcc tgagggatct actcgttccg ggcttg
//
LOCUS       DAOSZX010000094         5349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_12872_length_5349_cov_22.006611, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000094 DAOSZX010000000
VERSION     DAOSZX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12872_length_5349_cov_22.006611"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1510
                     /locus_tag="PK196_07125"
     CDS             8..1510
                     /locus_tag="PK196_07125"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HON41223.1"
                     /translation="MRFFDTTLRDGEQTPGVSLTPAEKLEIAAHLAGIGVHVIEAGSA
                     AASAGERESIRLIADAGLKAETCTYVRALPADIDLAADAGADSVHLVIPVSDLHISKK
                     LRKTREQVCEMAWSSVEYAKERGLIVELSGEDASRADQTFLAEIFREGVERGADRLCF
                     CDTVGLLTPERAAEIIPPLLFAPLSIHCHDDLGFALANTVAALRAGASAAHVTVNGLG
                     ERAGNTALEELVMALEVLYGVDTGIVTSELYPLSTHVARLTGVPLPTNKPIVGEMAFT
                     HESGIHAHGVMRDASTYEPLKPERVGRRRRIVLGKHSGSAAVEAALHDMGYNPNPAQL
                     AEIVARIKRMGDTGTRITDADIMTIADTVMEIEFKPCLELRQFTIVSGSNAMPTASVT
                     MLVNGEEITGAAVGNGPVDAAIRALQRSVADVGDVQLDEYRVDAITGGTDALVDVSVK
                     LRKDGKSVTSRGARTDIIMASVEAVITGMNRLLREEHEDRSQDSDRSAAA"
     gene            1467..3143
                     /gene="ilvB"
                     /locus_tag="PK196_07130"
     CDS             1467..3143
                     /gene="ilvB"
                     /locus_tag="PK196_07130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HON41224.1"
                     /translation="MKTGARTLIEALQREGVDTIFGYPGGVVLPIYDELYDSSIRHIL
                     VRHEQAAAHAADGYARASGRVGVCLATSGPGACNLVTGIATAYMDSIPIVALTGQVPT
                     ALLGNDAFQESDITGITMPITKHNYLVKNAADIDRVVKEAFYIAGTGRPGPVLIDLPK
                     DVSMGEVDEASSPPAAISLRGYQPTYEGHVRQIDKALDLIAEAERPLVYAGGGVILSG
                     ASAELREFIETAAIPVTTTLMGLGAIPGDHPLNLGMLGMHGTAAANFAVTECDLLVAI
                     GVRFDDRVTGKIEAFAPNARIIHIDIDPAEIGKNKKVDVPIVGDVGRVLRAMLARMKK
                     RGDTANWVNRAAAWKAQFPPSYRDDDRLRPQYIIQQLSDLMKGEGIVTSEVGQNQMWT
                     ALYYCFRKPRSWITSGGLGTMGYGFPAAIGAHFARPDETVVDVAGDGSFQMNIQELAT
                     VAHNNIPVKVVILNNMYLGMVRQWQELFYDRRYAYTELSPVDFVKVANAYGVEGIRVE
                     EKADVRGALETALSHDGPFVLDFRIEREENVFPMVPAGAAINEMIGVRNP"
     gene            3140..3643
                     /gene="ilvN"
                     /locus_tag="PK196_07135"
     CDS             3140..3643
                     /gene="ilvN"
                     /locus_tag="PK196_07135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HON41225.1"
                     /translation="MTSHTLSVLVENRAGVLSRVAGMFSRRGFNIESLAVGTCEEPGM
                     SRITIVVNGDDAVVEQVMKQTNKIIDVIKVSDLTERESVERELALIKVAAETGAARAE
                     IMQIANIFRAQIVDVGTRTLVLQVAGDTDKIEALEKLLRQYGIKELVRTGKVALLRGS
                     KTVKTSK"
     gene            3838..>5349
                     /locus_tag="PK196_07140"
     CDS             3838..>5349
                     /locus_tag="PK196_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HON41226.1"
                     /translation="MADLATVALIGIYFFVLLVIGTWASHKIRNTEDYILAGRSLGFW
                     IFTILIVCSICSGMTLLGVSGFGYTSGWPGIWEQIFVPLAASFCIIFFGVKLNAVGKE
                     RGYMTVQDYLAERFESPRGMRALSAVSGILVSLIYLIGQYAAISIVLVWIFAIPHWQA
                     LLISGVIIIAYTVIGGLYAVSWTTLFQGGILIVGVLAMAPFVIRSAGGLTHINTVLAG
                     IDPNFVEPWFPSPAYASYAYATPEFLFSFAILLMVGLACAPHVINNVLAAKESRYFKW
                     SPLVAFGIYAVVMFLVKFTGFATRVLVEEGAVALPDVVNAQDYAFIVGVEYAMPNMFL
                     WGIFAVIVLAAVMSTTDRLMLTIGTSFSWDIYKNLFRPAASEKEVLMVSRVAVVVGAV
                     GTLILAINPPPMLAWLIWMGIGVMLATFAVPLLAGLYWRGATGAGGIASMATGLVAAG
                     VFGYWHQFVAPLPMHFSFFALLLSLLAMVVVSLLTRKNSPEILDGTRTGWFIQS"
BASE COUNT         1008 a         1540 c         1751 g         1050 t
ORIGIN      
        1 tgaaccgatc cgcttttttg ataccacgtt acgggacggc gagcagacac cgggtgtcag
       61 cctgacgccg gccgagaagc ttgagattgc agcccacctt gccggtatcg gtgtccacgt
      121 catcgaagca ggctccgcag ccgcatccgc cggagagcgt gaatccatcc gccttatagc
      181 agacgccggg ctcaaagccg agacctgcac ctacgtccgg gcgctgccgg cggacatcga
      241 cctcgccgcc gacgccggtg cagactccgt ccacctggta atcccggtga gcgacctcca
      301 catcagcaag aaactccgca agacccgcga acaggtctgc gagatggcct ggagcagcgt
      361 cgagtacgca aaagagcgcg gactcatcgt cgaactctcc ggagaagatg catcccgcgc
      421 cgaccagacg ttcctggcag agatcttccg ggaaggagtc gaacgcggcg cagaccgtct
      481 ctgcttctgc gacaccgtcg ggctcctcac gcccgaaagg gcggccgaga tcatcccgcc
      541 gctactgttt gccccgctct ccatccactg ccacgacgac ctcgggtttg cgcttgcaaa
      601 caccgtcgcc gcgctgcgtg ccggagcatc cgccgcccac gtcaccgtca acggcctcgg
      661 cgaacgggcg gggaacaccg ccctcgaaga actggtgatg gcgctcgagg tcctctacgg
      721 cgtcgacacc gggatcgtga ccagcgaact ctaccccctc tcgacccacg tcgcccgtct
      781 gaccggggtg ccgctgccga ccaacaaacc catcgtaggc gagatggcgt tcacccacga
      841 gagcgggatc cacgcccacg gcgttatgcg ggacgcaagc acctacgaac ccctcaaacc
      901 cgagagggtg ggaagaagga ggcgcatagt cctcgggaaa cactcggggt ctgcagcggt
      961 cgaggccgcg ctccacgaca tgggctataa ccccaatccc gcgcagcttg ccgagatcgt
     1021 cgcaaggatc aaacggatgg gtgacaccgg aacacggatc accgacgccg acatcatgac
     1081 gatcgccgat accgtcatgg agatcgagtt caaaccctgc ctcgaactgc ggcagttcac
     1141 catagtctcc gggagcaacg ccatgcccac cgcctccgtc acgatgctcg tcaacggcga
     1201 ggagatcacc ggtgccgcgg tcgggaacgg ccccgtggat gcggccatcc gcgcgctaca
     1261 gcgatcggtc gcggatgtcg gggatgtgca gcttgatgaa taccgtgtcg acgccataac
     1321 cgggggcaca gacgccctcg ttgacgtctc cgtgaaactg aggaaggacg gaaagagcgt
     1381 cacctcccgg ggcgcccgga cagacataat catggcaagc gtcgaagcgg ttatcactgg
     1441 tatgaacaga ttactcaggg aagagcatga agaccggagc caggactctg atagaagcgc
     1501 tgcagcgtga aggggtggat accatattcg gctaccccgg cggcgttgtg ttgccgatct
     1561 acgatgaact ctacgactcg tcgatccggc acattctggt aaggcatgag caggcagcgg
     1621 cgcacgcggc cgatggctac gcgcgtgcaa gcggccgcgt aggtgtatgc cttgccacct
     1681 ccggccccgg cgcctgcaac cttgtaaccg gtatagcgac ggcctacatg gactccatcc
     1741 cgatcgtcgc cctcaccggc caggtgccca cagcactcct gggtaacgac gctttccagg
     1801 agtcggacat caccgggatc acgatgccga tcacgaagca caactacctg gtgaagaacg
     1861 ccgccgatat cgaccgcgtt gtaaaggagg cgttctacat cgccggcaca ggccggccag
     1921 gcccggtgct gatcgatctc ccaaaagacg tcagcatggg cgaggtggat gaggcatcct
     1981 cgccgccggc ggcgatctcg cttcggggct accagcccac gtatgagggg cacgtccgcc
     2041 agatagacaa ggcgcttgac ctgatcgcgg aggcggagcg gccgctcgtc tacgccggcg
     2101 gaggcgtgat cctctccggc gcctcggccg aactccgtga gtttatcgag accgccgcca
     2161 taccggtgac aaccaccctt atggggcttg gcgccatccc gggagaccac cccctcaacc
     2221 tggggatgct cggtatgcac ggcaccgcgg ccgcaaactt cgctgtcacg gagtgcgatc
     2281 tcctggtggc aatcggggtg cggttcgatg accgggttac aggcaagatc gaggccttcg
     2341 ccccgaacgc ccggatcatc catatcgata tcgaccccgc cgagattggg aagaacaaga
     2401 aggtcgatgt accgatcgtc ggggatgtcg ggagggtgct ccgggcgatg cttgcgcgga
     2461 tgaagaagcg cggcgatacg gcgaactggg tgaaccgggc ggccgcctgg aaggcgcagt
     2521 tcccgccgag ttaccgcgac gacgatcgcc tgcggccgca gtacatcatc cagcagctct
     2581 ccgacctgat gaagggcgag gggatcgtca caagcgaggt cggccagaac cagatgtgga
     2641 cggcgctcta ctactgtttc aggaaaccgc ggtcctggat aacctcgggc ggtctcggga
     2701 cgatgggcta cgggttcccg gcggccatag gcgcccactt tgcccggcct gatgagacgg
     2761 tcgtggatgt cgcgggtgac gggagtttcc agatgaacat acaggagctc gcgacggttg
     2821 cgcacaacaa catcccggta aaggttgtca tactgaacaa catgtacctc gggatggtcc
     2881 ggcagtggca ggagctcttc tacgaccggc gttacgccta caccgagctc tcgccggtgg
     2941 actttgtgaa ggttgcaaac gcctacggtg tcgaggggat acgggtcgag gagaaggccg
     3001 atgttcgcgg ggcgctcgag acggctctct cacacgatgg gccgtttgtc ctggatttca
     3061 ggatagagcg ggaggagaac gtctttccca tggtcccggc cggtgctgcg atcaacgaga
     3121 tgataggggt gcgcaaccca tgacatcgca tacgctgagc gtcctggtcg agaacagggc
     3181 gggtgtcctc tcccgggttg ccgggatgtt ctcacgccgg gggttcaaca tcgagagcct
     3241 tgccgtgggg acgtgcgagg agcccgggat gagccggatc acgatcgttg tgaacgggga
     3301 tgatgctgtg gtcgagcagg tgatgaagca gaccaacaag atcatagacg ttatcaaggt
     3361 ctccgacctg acggagcggg agagcgttga acgagagctt gcgctgatca aggtggccgc
     3421 ggagaccggg gccgcccggg cggagatcat gcagatcgca aacatattcc gggcgcagat
     3481 cgtggatgtg gggacgagga cgctggtgct ccaggttgcg ggggatactg ataagatcga
     3541 ggccctggag aaactcctgc ggcagtatgg catcaaggag ctcgtcagga cgggcaaggt
     3601 tgccctcctc cgcgggtcaa agactgttaa aacctctaaa taactttttg tgcggtgtga
     3661 atggcgatcg gagcgatctc cgatcgattg tgttatatgt tagcatgcca cacgctgtca
     3721 cagcatgacg atacttggtt taccactcca cacgatactc tatgtgggcg caaccgtcgt
     3781 cctggccttt gtgttgttga tcatatgggg tctgcggttc caggaggatg agtcctgatg
     3841 gcggatctcg cgacggtcgc cctgatcggg atttacttct ttgtgctcct ggttataggc
     3901 acctgggcgt cgcacaagat ccggaacacc gaggactaca tccttgcggg gaggtcgctc
     3961 gggttctgga tcttcacgat cctgatcgtc tgttcgatat gcagcgggat gacgctgctc
     4021 ggggtcagcg ggtttgggta tacttcgggc tggccgggga tctgggagca gatctttgtg
     4081 ccgcttgcgg catcgttctg tatcatcttc ttcggggtga agctgaacgc tgtggggaag
     4141 gagcggggct acatgaccgt ccaggactac ctggccgaga ggtttgagag tccgcggggg
     4201 atgcgggctc tctcggcggt ctcggggatc ctggtctcgc tgatatacct tatagggcag
     4261 tatgctgcga tcagcatcgt gctggtctgg atatttgcga taccgcactg gcaggcgctg
     4321 ctgatctcgg gtgtcataat catagcctac acggtgatcg gggggttata cgcggtgtcc
     4381 tggacgacgc tcttccaggg cgggatcctg attgtggggg tgctcgcgat ggcgcctttt
     4441 gtcatcagga gcgcgggggg gcttacccac atcaacacgg ttcttgcggg gatcgatccg
     4501 aactttgttg agccctggtt cccgagcccg gcatacgcgt cttacgccta cgcgacgccg
     4561 gagttcctct tctcgtttgc catactgctg atggtggggc ttgcgtgtgc gccgcacgtc
     4621 atcaacaacg ttctggcggc gaaggagtcg cgctacttta agtggtcgcc gcttgtggcg
     4681 ttcgggatat acgcggttgt gatgttcctg gtgaagttta cggggtttgc aaccagggtg
     4741 cttgtggagg agggggcggt ggcccttccc gatgttgtga acgcccagga ttatgcgttc
     4801 attgtgggtg tggagtatgc gatgccaaac atgtttttat ggggcatttt tgctgtgatt
     4861 gtgcttgcgg cggtgatgtc gacgacggat cggctgatgc tcacgatagg gacgtcgttt
     4921 agctgggaca tctacaagaa cctcttccga ccggcggctt cggagaagga ggttctcatg
     4981 gtctccaggg tcgcggttgt ggtgggggcg gtggggacgc ttatcctggc catcaatccg
     5041 ccgccgatgc tggcctggct gatctggatg ggtatcgggg ttatgctggc gacgtttgct
     5101 gttccgctgc tggccggcct ctactggagg ggggcgacgg gtgcgggcgg tattgcgagc
     5161 atggcgaccg ggcttgtggc ggccggggtc tttggctact ggcaccagtt tgtggcgccg
     5221 cttccgatgc acttcagttt ctttgcgttg ctgctctcgc tgctggcgat ggttgttgtg
     5281 agtcttctga cgcgaaagaa ctctcccgaa atactggatg gcacccggac gggctggttt
     5341 atccagtcc
//
LOCUS       DAOSZX010000095         5338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_12898_length_5338_cov_12.492902, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000095 DAOSZX010000000
VERSION     DAOSZX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_5338_cov_12.492902"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK196_07145"
     CDS             complement(<1..1016)
                     /locus_tag="PK196_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HON41227.1"
                     /translation="MNGKKYELTKEILDSLRDVEGFPIGKDEDIIALSDPPYYTACPN
                     PWIGEFIERYGRPYDPEERYEREPFAADVREGKNDPIYNAHSYHTKVPHKAIMRYILH
                     YTDPGDIVFDGFCGTGMTGVAAQMCGNPDPEFRERIEREMPGVEWGARRAILCDLSPA
                     ATFIAYNYNNPVDPEEFEREAKRILAEVEKECGWMYETNHLVDGKVQFKADLDGTRTP
                     VKGRINYTVWSDVFVCPHCSGEIVFWEAAVDQKEGKVRDEFPCPHCGAMMTKRSLERA
                     WEYVHDEAIGETVRRAKQVPVLINYSVGKKRFEKVPDENDLALIQRIEGGRIPYWYPT
                     DELP"
     gene            complement(1019..4699)
                     /locus_tag="PK196_07150"
     CDS             complement(1019..4699)
                     /locus_tag="PK196_07150"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HON41228.1"
                     /translation="MKYGDLVRFDPIEDVVQLREADKKDEARRLVETYVISDGMAKNL
                     IEVLIPHLELNTTLDKKGLLVVGNYGTGKSHLMSVVSAVAEHADLVEHLQSDDIKEPV
                     KKIAGQFKVIRTEIGAVRQSLRDIVCGELERNLAKIGVDYSFPPADRLVNHKDAFIAM
                     MNAFNEKYPDKGLLLVVDELLDYLRSRQEQELILDLNFMREIGESCRLSRFRFMAGIQ
                     EALFDNPRFEFVAESMRRVQKRFESVRIVRTDVSYVVAERLLKKTPEQKAMIRHHLGK
                     FTALYGDMNERLDEYVALYPIHPAYLEIFEKLFVVEKREILKTLSRDVKKLIEEEVPT
                     DSPGLLSYDHYWEPLSTDTSLRTNQDIREVIEKGQILEGILNRSFQRKQYLPSAIRIV
                     HALCVHRLTTGDVSSPIGLTPEEIRDDLCIYLPIPEQDSEFLLTTIESILREIGKTVS
                     GQFISHNDENDMYYIDVRKDIDYDAKIEERADSLDKGILDRYYFEVLAGVMKTPGSTY
                     VPGYRIWEHEVIWRERRAGRSGYLFFGAPNERSTAQPPRDFYIYFLQPYDPPLFTDEL
                     RSDEVFFRLSRMNETFETALRLYAGAREMFTNSSGSNRSIYGAKASEHLKTITKWLRE
                     NIITAYDVTYQGVTRPLVELAKEGGINTSWDISDTINDISAYCLAPHFEEIAPDYPHF
                     SQVIRSKEREEAMKDAIKCISGVRVQRGIAVLDGLNLLEDGSINTENSVYARHINDLL
                     RSKGVGQVLNRSEIIGGQRGVEYDLRFRLEPEFVVVVIAAMVYHGDVVVAYPGKKITA
                     ANFEELTRMSVADLTAFKHLELPKEPPIEVLAELFSLLEISPALVRNSATHREAVKQL
                     QERVAGLIEEVVEAKEVLKSSLTLWGQSVLDESRKEDAARKLDDLKVFLESLQRFKTE
                     GQLRNFGSSRADVRKQKKNLEVMREIGDLAKFCLRVAPVTQYLAEAERVLPDDNPFLQ
                     SLVEQKKALLEKIRDPETQKTKEFQREFERELNRLKKEYIDEYLRLHGAARLGVEGDN
                     RKKRLMKSPALKAIRELSSIEIMPKGDLTGFEAELTRLKPCFALDSGALENSVTCPYC
                     QFRPADEEISAPLENILSALEDRLDDLYDRWTQTLLENLSDPAVSTSLEALKPDERKL
                     VEDFMAAKELPEKISVEFVKAMQEVLSGLVKVTITRDALMDGLKGNGSPCTVDEFRNR
                     FEGYIKSLTRGRDERKIRIVVE"
     gene            complement(4696..5178)
                     /gene="brxF"
                     /locus_tag="PK196_07155"
     CDS             complement(4696..5178)
                     /gene="brxF"
                     /locus_tag="PK196_07155"
                     /inference="COORDINATES: protein motif:HMM:NF033453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HON41229.1"
                     /translation="MIREALDSVSLKNTRLIILTGRSGREISAVLRNCEDQFGFPRIN
                     VNTELSRGLLDLPPCDRPIMAERLLHEIIMKVEGEAVLLDRTEVLFSRDLALDPFRLL
                     SNLGRYKTVIAAWNGDYDGSELVYAYTGHDDYRRYGRNHLGDTPILSVSSNGRGCVVA
                     "
BASE COUNT         1172 a         1633 c         1267 g         1266 t
ORIGIN      
        1 tcaggcaact catccgtcgg gtaccagtac gggatcctcc ccccctcgat cctctggatc
       61 agggcaaggt cgttctcgtc cggcaccttc tcaaaccgtt tcttcccgac cgagtagttg
      121 atcaagaccg gcacctgctt tgcccgccgc accgtctcgc cgatggcctc gtcatggacg
      181 tactcccatg cccgctcgag cgagcgcttc gtcatcatcg cgccgcagtg cgggcagggg
      241 aactcgtcgc ggaccttccc ctccttctgg tccaccgccg cctcccagaa gacgatctcc
      301 cccgaacaat gcgggcagac aaagacatcg ctccagaccg tgtagttgat ccgtcccttc
      361 accggggttc tggtgccgtc caggtcggct ttaaactgga ccttcccatc cacgagatgg
      421 ttcgtctcgt acatccagcc gcactccttc tccacctcgg caaggatccg tttcgcctcc
      481 cgctcgaact cctccgggtc caccgggttg ttgtagttgt aggcgatgaa ggttgccgcc
      541 ggcgagaggt cgcagaggat cgcccggcgg gcaccccact ccacaccggg catctcccgc
      601 tcaatcctct ccctgaactc cgggtctgga ttgccgcaca tctgtgctgc aacacccgtc
      661 atccccgtcc cgcagaaacc gtcaaagacg atatcgccgg gatcggtgta gtgaagaatg
      721 tagcgcatga tcgccttgtg cggcacctta gtgtggtagg aatgagcgtt gtagatcgga
      781 tcgttcttcc cctccctcac gtcagccgca aacggctccc gctcatacct ctcctccggg
      841 tcatacggcc gcccgtagcg ctcgatgaac tccccgatcc agggattcgg gcacgcagtg
      901 tagtagggcg ggtcggaaag cgcaatgata tcttcgtcct ttccaattgg gaacccctca
      961 acatcacgca gagagtcaag aatctccttt gtcaactcgt attttttacc gttcatccct
     1021 actccaccac aatacggatc ttccgctcgt ccctgcctct tgtcagggat ttgatgtaac
     1081 cctcaaaccg gttccggaac tcgtccaccg tgcagggcga cccattcccc ttcaggccat
     1141 ccatcagagc atccctggtg attgtcacct tcacaagccc cgaaagaacc tcctgcatcg
     1201 ccttcacaaa ctcgacagat atcttctccg gaagctcttt tgccgccata aaatcctcaa
     1261 ccagtttcct ctcatcgggt ttcagggctt caaggcttgt gctgaccgcc gggtcgctga
     1321 ggttctccag cagtgtttgc gtccacctgt cgtagagatc atccagcctg tcctcaagcg
     1381 ccgaaagaat gttctccagt ggcgcggata tctcctcatc cgccggcctg aactgacagt
     1441 acgggcaggt cactgagttc tcaagagcgc cactatccag cgcaaagcac ggcttcaggc
     1501 gtgtcagttc cgcctcaaaa ccggtcagat cacccttcgg catgatctcg atgctcgaga
     1561 gctcccggat tgccttgagg gcaggactct tcattaacct cttcttccgg ttgtccccct
     1621 cgacacccag gcgcgccgca ccatggagac gcaggtactc gtcgatgtac tccttcttca
     1681 accggttcag ttcccgttcg aactccctct ggaactcttt ggtcttctga gtctccggat
     1741 ctctgatctt ctccaacagg gcttttttct gctcaaccag cgattgcaga aaagggttat
     1801 cgtcaggcag gaccctctct gcctctgcga ggtattgagt aaccggcgcg acacgcagac
     1861 agaactttgc cagatccccg atctcccgca taacctccag gttctttttc tgcttgcgca
     1921 catcagcccg gctgctgccg aagttccgga gttgcccctc cgtcttgaac cgctggagcg
     1981 actcgaggaa gaccttaagg tcatcaagtt tccgggcagc atcctctttt ctgctctcgt
     2041 caagaacgct ctgcccccat agcgtcagag aactcttcaa cacctcctta gcctcaacaa
     2101 cctcttcgat caacccggcc acgcgttcct gcaactgctt caccgcttcc cggtgggttg
     2161 cggagttccg gacaagtgct ggtgatatct cgaggagaga gaagagttcg gcaagcacct
     2221 cgatcggcgg ttcttttggc agttcaaggt gtttaaacgc tgtcaggtca gcaacactca
     2281 tcctggtgag ttcctcaaaa tttgcggctg taatcttctt ccccggatac gccaccacca
     2341 catccccatg gtataccatg gcggcaataa caacgacaac aaactcaggc tcaagccgga
     2401 accgcagatc atactcgaca cccctctggc cgccgatgat ctcgctccgg ttcagcacct
     2461 gaccaacccc cttgctccgg aggagatcat tgatgtggcg ggcatatacg gaattctcag
     2521 tattgataga accgtcttca agcaggttga gaccgtcaag aactgcaatg ccgcgctgta
     2581 cgcggacacc gcttatacac ttgatggcat ccttcatcgc ctcttcccgc tctttagacc
     2641 gtataacctg cgagaaatgg gggtaatccg gggcgatctc ctcaaagtgg ggcgcaagac
     2701 agtaggctga gatgtcattg atggtatccg atatatccca cgaagtgttt atccccccct
     2761 ctttcgccag ctcaaccagg ggccgggtca ccccctggta cgtcacgtcg tatgcggtga
     2821 tgatgttctc ccgcagccac tttgtgatcg tcttgaggtg ctcggacgcc ttggctccgt
     2881 atatgctgcg gttcgacccc gaagagttag tgaacatctc cctcgcgccg gcatataacc
     2941 ttagagcagt ctcgaaagtc tcgttcatcc tgcttaacct gaagaagacc tcatccgacc
     3001 gcagttcgtc cgtgaagagc ggcggatcgt atggctggag gaagtagatg taaaagtcgc
     3061 gtggcggctg ggcggtcgac cgttcgttgg gcgctccaaa gaagaggtag ccagacctcc
     3121 cggctcgccg ctccctccag atgacctcat gctcccagat ccggtagcct ggcacatagg
     3181 tcgatcccgg tgtcttcatc accccggcca ggacctcgaa atagtagcga tccagtatcc
     3241 ctttatccag ggaatcggcc cgctcttcga tctttgcgtc ataatcaatg tccttccgaa
     3301 catcgatgta atacatatcg ttctcatcat tatgcgatat gaactgcccg ctgacggtct
     3361 ttccgatctc acggagtatc gactcgattg tggtgagcag gaactccgaa tcctgctccg
     3421 gtatagggag atagatacag agatcatccc ggatctcttc cggcgtcaac ccaatcgggg
     3481 aactcacatc gcctgtcgtg agacgatgga cgcagagggc atgaacaatc ctgatcgcag
     3541 atgggaggta ttgtttcctc tgaaacgatc ggttcaggat cccttcaagg atctgacctt
     3601 tttcgataac ctcccggata tcctgatttg tccgcaacga cgtatcggtg ctgagaggtt
     3661 cccagtagtg gtcgtaagag agcaggcccg gactgtctgt tgggacctcc tcttcaatga
     3721 gttttttaac atccctgctc aacgtcttga ggatctccct tttctcaaca acaaaaagtt
     3781 tctcgaagat ctccaggtat gccgggtgga tggggtagag agcaacgtat tcatcaagcc
     3841 gttcgttcat atccccatac aatgcggtga actttccaag atgatgccgg atcattgcct
     3901 tttgttctgg cgtcttcttc agcaaccgtt cagccacaac atacgagaca tccgtccgga
     3961 ctatccgcac cgactcaaac cgcttctgga ccctcctcat tgactcagca acgaactcaa
     4021 accgcgggtt gtcaaagagc gcctcctgga tcccggccat aaaccggaac ctgctcagac
     4081 gacaggattc accgatctca cgcataaaat tgagatccag tatcaactcc tgctcctgcc
     4141 gtgaacgcag atagtcaagg agttcgtcaa cgacgagaag caaccctttg tcggggtatt
     4201 tctcgttgaa tgcattcatc atcgcgataa aggcatcctt gtggttcacc aatctgtctg
     4261 caggaggaaa cgagtagtcc acaccaatct ttgccaggtt tctctcgagt tcaccacaga
     4321 cgatatcacg aagcgattgc cgaacagctc cgatctctgt cctgataacc ttaaactgac
     4381 ctgcaatctt ctttacaggc tcctttatgt catcgctctg gagatgctcc acaagatccg
     4441 cgtgctctgc aacggcactg acaaccgaca tcaggtgcga cttaccggta ccataattcc
     4501 caacaaccag cagccctttc ttgtcgagcg ttgtgttcag ttcaaggtga ggtataagaa
     4561 cctcgatgag attcttcgcc atcccatccg agatgacata tgtctccaca aggcgccggg
     4621 cctcatcctt tttatccgcc tctcgcaact gaactacatc ttcaatagga tcaaaccgaa
     4681 cgagatcgcc gtatttcatg ccacaacaca ccccctccca tttgatgata ccgataggat
     4741 cggggtatca ccaagatgat tcctgccata cctccggtag tcatcatggc cggtataagc
     4801 gtacacgagt tccgatccat cataatctcc gttccatgcg gcgattacgg ttttgtaacg
     4861 cccgagattg ctcagtaaac ggaatggatc gagagccagg tcgcgggaga acaacacctc
     4921 ggttctatcg agcagcactg cctcaccctc taccttcatg ataatctcgt gaagtaagcg
     4981 ttcggccata ataggacggt cgcaaggagg taaatcaagg agtcctcgac tcagctcagt
     5041 gttcacgttg atgcgtggaa agccaaactg atcttcgcag ttgcgcaaca cagcggaaat
     5101 ctctcttcct gaccgaccgg tcaggatgat taatctggta tttttaagcg aaaccgagtc
     5161 gagagcctcc cgaatcatca tacaatgcat gagaataaga accgataaat gtatctttaa
     5221 aatttaaatg tcgatacccc ccaggtgagt ttgtggctct ttggccagtg atcctggaat
     5281 caaaaactct gctcagaaag atctattgaa cagatctctc ccgacgatgg agaggggg
//
LOCUS       DAOSZX010000096         5219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13272_length_5219_cov_19.909566, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000096 DAOSZX010000000
VERSION     DAOSZX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13272_length_5219_cov_19.909566"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..3926
                     /locus_tag="PK196_07160"
     CDS             369..3926
                     /locus_tag="PK196_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41230.1"
                     /translation="MDKSIPIEERVKSAVTSGRPAELEEALREISTTRTRKGKRPLYA
                     QVNAAITRAAFERGDPGILNLITEPTGEEVIEASRCAMARYIDTADEAWFSAVFALVG
                     KINRKGHQSALLARISRDLVLLAVDTGKKQYIETAQKTLEAISIRKYRSEFLSETIPL
                     LIRYGEEHRNIEILHGVLAMLPEIKTLSERSRLRAGLARAIAGVGMVSEDPDLLIQGL
                     SVAAGIDQKVQRLSTISGIVNTAWRSPLKAEIADIENILDSLRETRQERLAEIVARLT
                     GELLDHHQDREDAYRQIVELAAKKPWTARIIVHELLARAERSGDGWFFEKVFEFGARN
                     ENSTLPPIEGIVSAGITIATRSGNPDVLHNILPLVDDCSDKATAATLYQRISETLYQV
                     GDFRQAVLVLKKAGNPHYTPALFQTSIKLIIEGIHHNETGFIRENLLAGEVNGIADSL
                     IQQAVTGFCRECSLDEAAGHMPAIKELAVVHQSQDRLLLDCGSILLERGFIQEKGPAA
                     LLDILNQIVDPGLRDEAFSKIAVEMARIGAARNDRRLLQDSTALACEIMEQKRRAGAL
                     ADIVNHVAALAIREDDPDLLQGMRILSTSLLAPDQCMIAIENIVQGLVTYGVKHRSLQ
                     ALDEAARILVQNPETHLQHLRETLIEGYIRVGCTHIVDCRSSVIRGSFEGILEPFETA
                     LTLLKTGAPPSEIQIRITDCIDIMLKQMQDSRDAVLVIPMALFSLEIENEHERTAMIQ
                     RILTPFTEQTQEFDSADPYEATAYLLEGIDGATASLPVLDLMHRLFKHTADLYSRYSG
                     VYRVASAYLVLGEAERAESIIRMLHETIDEIPDPAVRAIMLSDLAGLMAGIEPGAARD
                     YLAEAERMVGSAGEEREGLVRKHLVYAFREICAATDGKKDLDWAIEQGRRIEDPVDRV
                     DALSAVYDTADEPAVRKEVVSMICQAAAGIPSVYARLPMLFYAARFVGASGDEDAIER
                     ILENMERTTGFIRIPFITAITQLRMAGMLYHLYRTTGSISAMERATAIASAIEDERIR
                     YILMVQNDNITPDPWDGTVYGRILDSRAGFRRGDYTTKDVVALDRAIGAAPDRMKRAI
                     YYTEAYVFARDAGQHQIAGRMLDCALKEAGGIRPLLRRAIALGDMACRLHAACYDDRA
                     GDLLDLAVNEVLNIRDRATRESIYDELDMSIRIIQEYWL"
     gene            3923..4201
                     /locus_tag="PK196_07165"
     CDS             3923..4201
                     /locus_tag="PK196_07165"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HON41231.1"
                     /translation="MKTIEISVAGRVQGIGFRACVKRIATNLGVVGEVMNLPDGRVRI
                     IATAEPGILDKFVSMLYSCPHVIIREIVQREIPLINFTEFSIQRSGYL"
     gene            complement(4192..5130)
                     /gene="cas1"
                     /locus_tag="PK196_07170"
     CDS             complement(4192..5130)
                     /gene="cas1"
                     /locus_tag="PK196_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HON41232.1"
                     /translation="MTEAGTWLPVFGYGGHIKATTQEVIISRGSKTSRYPLEKVKHLL
                     VVGGHTLHTSAVTNLLKSGAAITFFDIDGTPVGCLYPYGYRPDEAVRLAQERAAPHRF
                     AQPLSLAALQSRLLLLGEFSDRGGGEAIFYAGELEILHQVREELEISVTMEELRRLSR
                     LTADMYYEILARTIPPELGFRRRTTPPHTDPVNTMFSLGYAMLYGNCCVSVVGAHLNP
                     DRGMLHEGPGGLVYDLIEPQKASMVDRVVLRFAREEVSTDDYECGEKRCYLDPAIVNR
                     LTEALRDSIDQDRLDAQVRILRDALLSNAGFQVLYR"
BASE COUNT         1171 a         1474 c         1546 g         1028 t
ORIGIN      
        1 cccccgtatc tctattctcc gtgggatgcc gtctcgcaaa aaccccggcc accccggatc
       61 cggatgggcg cgacgattcg cggcgaagtt accaccccga atccttccat gcagggattt
      121 atcaaaccag gaagcaggct actggtttga tgccctcatt tcggcagcag ttatattggg
      181 aaaaaatatt ttactattat gatatactgg tcattaattt ttatgcgaac cgggcatttt
      241 agttactaaa ttgcttgcaa cgcgaaatat tacagaatgt caggaaataa aatttatata
      301 ggggtgcgac aatcctattg cttggtgatg gcgtatgcag ggtataccac ggggggccgc
      361 tggataagat ggataagagc ataccgatag aggagagggt aaaatccgcc gtaacctccg
      421 gccgccccgc cgaactcgaa gaagctctcc gcgagatctc gacgacacgc acaaggaaag
      481 gaaagcggcc gctctatgcg caggtgaacg ccgctatcac cagagcggct tttgagagag
      541 gagatccagg tatactgaac ctgatcaccg aaccaacagg agaagaggtt atcgaggcat
      601 cccgctgcgc catggcccgg tacatcgata ccgcagacga agcctggttt tctgccgtat
      661 ttgccctggt cggcaagatc aaccgcaaag gccaccaatc agcactgctc gccaggatct
      721 cacgtgacct tgttcttctc gcagtggata cggggaagaa gcagtatatt gagacagcgc
      781 aaaaaaccct cgaagccatc agcatccgca aataccgctc ggagttctta tccgagacca
      841 tccccctgct gatccgctac ggcgaggagc accgaaacat cgagatcctg catggcgttc
      901 ttgcaatgct ccccgagatc aaaacccttt cagagcgatc caggctccgc gctggattgg
      961 cccgggcgat tgccggggtt ggtatggtgt cagaggaccc tgacctcctg atccaggggc
     1021 tatctgttgc cgccggaata gaccagaagg tccagcgcct cagcaccatc tccggtatcg
     1081 tgaacaccgc ctggagatct cccctcaaag cagagatcgc tgatatagag aatatcctgg
     1141 actcgcttcg cgagacccgg caggaacggc tggcagagat cgtggcgaga ctgaccggag
     1201 aactcctcga ccaccaccag gacagggaag atgcatacag acagatcgtg gaacttgccg
     1261 ctaaaaaacc ctggacagcc aggataatcg tacatgaact cctggccagg gcggagcgga
     1321 gtggcgatgg atggttcttt gagaaggtgt ttgagtttgg tgcccgcaat gagaacagca
     1381 ccctgccacc catcgaaggt atcgtcagcg ccgggatcac catcgccacc aggagcggca
     1441 acccggatgt tctccataac atcctgccgc tggtagatga ctgcagcgat aaggcaacgg
     1501 ccgccacgct ttaccagcgg atcagcgaga ccctatacca ggtgggcgac ttccggcagg
     1561 cggttctcgt cctgaagaag gcgggtaacc cgcattacac cccggcgttg ttccagacca
     1621 gcatcaaact gatcatagaa ggtatccacc acaacgagac cgggttcata cgcgagaacc
     1681 tcctcgctgg agaggtgaac gggatcgccg actcactgat ccagcaggcg gtgaccgggt
     1741 tctgcaggga gtgcagtctc gatgaggctg ccgggcatat gccggccata aaggaactcg
     1801 cagtggtgca ccaatcacag gacaggcttc tcctcgattg cggcagcatt ctgcttgagc
     1861 gcggtttcat acaggagaaa ggacctgctg cactgctgga catcctgaac cagatcgtgg
     1921 atccgggtct gcgtgacgag gcgttctcaa agatcgcagt cgagatggca cggatcggcg
     1981 ccgccaggaa cgaccgccgc cttctccagg actcaaccgc tctcgcctgc gagatcatgg
     2041 aacagaagag gcgggcgggt gcgcttgccg atatcgtgaa ccacgtggcc gcgcttgcca
     2101 tccgggaaga cgaccccgac ctgctccagg gtatgcggat cctctcgaca tctctccttg
     2161 cacctgatca gtgtatgatc gcgattgaga acatcgtcca gggactggtc acctatggcg
     2221 tcaagcaccg ttcactccag gcgcttgatg aagcggcccg gatcctggtt caaaaccctg
     2281 aaacccacct gcagcacctc cgcgagaccc tcatcgaggg ttacatccgg gtcggttgca
     2341 cccatatcgt ggactgccgc tccagtgtca tacgggggtc atttgagggt atccttgaac
     2401 cctttgagac cgcgctcacc ctcctgaaga ccggggcacc gccgtcggag atccagatca
     2461 ggataacaga ctgcatcgat atcatgctca aacagatgca ggactccagg gacgccgtcc
     2521 ttgtgatccc tatggctcta ttctccctgg agatcgagaa cgagcatgag cggaccgcga
     2581 tgatccagag gatcctcacg cccttcacag agcagacaca ggagtttgac tccgccgacc
     2641 cctacgaggc gacggcatac ctccttgagg ggatcgatgg ggctacggct tcgctccccg
     2701 tgctcgacct tatgcaccgc ctcttcaagc acacagccga tctctattcg cgctactccg
     2761 gcgtctaccg ggttgcaagc gcctacctgg tcctcggcga ggcagagcgg gcggagagta
     2821 tcataagaat gcttcatgag accatcgatg agatccccga cccggcggtt cgtgcgatta
     2881 tgctctccga cctggcaggc cttatggccg gtatcgagcc cggagcagcg agagactacc
     2941 tcgctgaagc ggaaaggatg gttgggtctg cgggagagga gagggaaggt ctcgtcagaa
     3001 agcatctggt ctatgccttc agggagatct gtgctgcaac cgatgggaag aaagacctcg
     3061 attgggccat cgaacagggc cggaggatcg aagacccagt cgaccgcgtg gatgcgcttt
     3121 cggcggtcta tgatacggcc gatgaacctg cggtgagaaa ggaggtcgtc tcgatgatct
     3181 gccaggccgc agcaggcata ccctctgttt atgccagact cccgatgctc ttttatgcgg
     3241 cgcggtttgt cggggcatcc ggcgatgagg atgcgatcga acggatcctg gagaacatgg
     3301 agagaaccac cggtttcatc aggattccgt tcataacagc cataacgcag ttgcggatgg
     3361 ccggtatgct ttaccacctc taccgcacaa ccgggagcat atccgccatg gaacgggcaa
     3421 ccgctatcgc ctccgccatt gaggatgagc ggatccgcta tatcctgatg gtccagaacg
     3481 ataatatcac gcccgaccca tgggatggta cggtctatgg caggatcctg gactcccgcg
     3541 cggggttccg acgcggcgac tacaccacaa aagatgttgt cgcactcgac cgtgcgatag
     3601 gcgcagcacc cgaccggatg aagagggcga tctactacac tgaggcctac gtcttcgccc
     3661 gcgacgccgg gcagcaccag atcgcgggga ggatgctgga ttgtgcgtta aaggaggctg
     3721 gcgggatcag gcctctcttg cggcgtgcga tcgcgctcgg agacatggcc tgccgtctcc
     3781 atgcagcgtg ttacgatgac cgcgcaggcg atcttctgga tctggcggtg aacgaggtgc
     3841 tgaacatccg tgaccgggca acaagagaat cgatatatga cgaactggat atgtccataa
     3901 ggatcattca ggagtactgg ctgtgaagac gatcgagatc agcgttgcag gacgggttca
     3961 gggcatcggt tttcgcgcat gcgtcaaaag gatcgccacc aatctcggtg tcgtgggtga
     4021 ggtgatgaac ctcccggacg gcagggtgcg aattatcgca accgccgaac cgggcatcct
     4081 ggataagttc gtctccatgc tctacagttg cccgcatgtc ataatcaggg agatcgtcca
     4141 gcgggagatc ccgctgatca acttcacaga gttcagcatc cagcggagcg gttacctgta
     4201 gagcacctga aacccggcat ttgagaggag agcgtcgcgc aggattctga cctgggcatc
     4261 aagacgatcc tggtcgatgg aatcgcggag tgcttcggtc agccggttca caatagccgg
     4321 atcgaggtag cacctcttct ccccgcactc gtaatcgtca gtggatacct cctcccttgc
     4381 aaaccgaagg acgacccggt ctaccattga ggctttctgc ggttctatga ggtcatacac
     4441 cagaccgccg gggccttcat ggagcatgcc ccggtcggga ttgaggtgcg ctccgaccac
     4501 cgagacacag cagtttccat acagcatagc ataaccgagc gagaacattg tgttgacggg
     4561 atcggtgtgc gggggggtcg tcctgcgccg gaacccgagt tcaggcggga tggtgcgggc
     4621 caggatctcg tagtacatgt cggcggtgag gcgcgagagc ctccgcagtt cctccatcgt
     4681 gaccgagatc tcgagctcct ccctgacctg gtggaggatc tcgagttccc ctgcataaaa
     4741 tatggcctca ccgccgccgc ggtcagagaa ttctccaagg aggagaagcc tggactggag
     4801 agcggccaga gagagcggct gggcaaacct gtgtggtgcg gcccgctcct gggcaagcct
     4861 tacagcctcg tccggccggt agccgtaggg gtagagacag ccgacgggcg tgccgtcgat
     4921 atcgaaaaat gtgattgcag cgccggattt gagaaggttt gtcaccgcgg aggtgtggag
     4981 ggtatgcccg ccgactacca ggaggtgttt aaccttctcg agagggtaac gagaggtctt
     5041 gctcccgcgt gatatgatga cctcctgtgt cgttgccttg atgtgtcccc catacccgaa
     5101 tacagggagc caggttcctg cctctgtcat cgacgcaccc cggaaaggag tattggggag
     5161 ttttgggtga ttaatgcatc gattggccgg atatggaagg tgtttatggt gcccggcgg
//
LOCUS       DAOSZX010000097         5193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13354_length_5193_cov_22.221098, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000097 DAOSZX010000000
VERSION     DAOSZX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13354_length_5193_cov_22.221098"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..562
                     /locus_tag="PK196_07175"
     CDS             104..562
                     /locus_tag="PK196_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HON41233.1"
                     /translation="MRVMVGGTFDPLHAGHRKLLSRSFDLAGPAGEVVIGLTTDEFAG
                     TKEHPVRPYRERLNNVTAFILEKGYTASWAVEPLSDRYGSAVDADFDILVVSEETFHV
                     ALDINELRRQNGLAKVDLYVVSCVLAEDERRISSTRIYRGEIDPHGRLIR"
     gene            complement(555..1022)
                     /gene="pyrI"
                     /locus_tag="PK196_07180"
     CDS             complement(555..1022)
                     /gene="pyrI"
                     /locus_tag="PK196_07180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HON41234.1"
                     /translation="MSRKDTAHGLLVSPIRNGTVIDHITAGEALNVLRILGITGSTNE
                     CLSIATNVESRRLGKKDIVKIENRELRKEEVDRIALLAPQARINIIREYEVVEKKGVE
                     IPRVIKGVVRCPNPGCITNTNEPVESTFEVLDKGLHCLYCDWLIKGNIANHII"
     gene            complement(1019..1915)
                     /gene="pyrB"
                     /locus_tag="PK196_07185"
     CDS             complement(1019..1915)
                     /gene="pyrB"
                     /locus_tag="PK196_07185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HON41235.1"
                     /translation="MYHIISIRDFEREDLDHLLDRAEVFETGRYTPGLLKDRLVALLF
                     FEPSTRTRMSFAAALARLGGQSISVDSVEASSIVKGETLADTIRVVSGYVDAIVLRHP
                     KEGAARLAAEFSSVPVINAGDGAGQHPSQTLLDLYTIRKSMPLDGIDAGLLGDLRYGR
                     TAHSLALALSLYGVTLHTIAPTGLEMPVNIAVELRERGVEVVEHASVEEAIRELDVLY
                     VTRIQRERFPDTASYYNVASSYRITTQLLEGVRDHLMILHPLPRAGEIDPAVDRTPYA
                     RYFEQARNGVPVRMALLHEVMR"
     gene            2070..2261
                     /locus_tag="PK196_07190"
     CDS             2070..2261
                     /locus_tag="PK196_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41236.1"
                     /translation="MVPGEKEGYAAETCSHCGGLGCTYCSGKGSVMVEQPSRRCRHCG
                     GDGCIYCGYTGWEHPLKEY"
     gene            complement(2267..2992)
                     /locus_tag="PK196_07195"
     CDS             complement(2267..2992)
                     /locus_tag="PK196_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HON41237.1"
                     /translation="MIETGERLLLVGEDREYFVTAGEGQFSTDRGTIDLSALPGMNPG
                     GEVRTHLGVPFIVLRPRPTDFFVHAKRSGAPMLPKDIGLVIAYTGMNRDDTVLDAGTG
                     SGVAAIYFGKIARRVKTCEVRPEFAALAEKNIRNARLDNVEVMAGDMLQATGEFDVVH
                     LDMTITPAHVEHAFSLLRPGGYLACYTPFLEHTFTALDAAAPIFREVHCYECIERELT
                     RSARGTRPSTRVAHSGYITIARR"
     gene            complement(2989..3330)
                     /locus_tag="PK196_07200"
     CDS             complement(2989..3330)
                     /locus_tag="PK196_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HON41238.1"
                     /translation="MYPGKINPKKMKQMMKQMGMEMEELEGVERVIIQTSAGDYIFDE
                     AQVVATRMQGLTTYQITGEPRFEEAAPEIPDEDVALVASQTGATEEAALAALIETHGD
                     IAEAILKLAEQ"
     gene            complement(3312..3812)
                     /locus_tag="PK196_07205"
     CDS             complement(3312..3812)
                     /locus_tag="PK196_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HON41239.1"
                     /translation="MASTGYSKDSLLQRVRTIGDFQFGRGAGEAIFPDGCTFQLSTTG
                     RIRQVRLDGERLATVRAQDGRLTLGIAGAVRLAAHIPPPGYRVAVQEDVAAFIADGRN
                     AMAKHVVSADEGIRAGDEVLVVTGDDNLIATGAAILSGREMLAFNYGVAVKIRQGRGS
                     ECIQER"
     gene            complement(3754..5151)
                     /locus_tag="PK196_07210"
     CDS             complement(3754..5151)
                     /locus_tag="PK196_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HON41240.1"
                     /translation="MNLERDLRRAAEIISDADSVTIVSHIDADGISTEAILAQALSRE
                     GIPVESVFVRQLEPMAMRHIPDDDSLKLFADLGAGQQALLESRGLTAEDVLILDHHVS
                     QPCTTAYPMINCLDHGITRMSAAGLAYLVAKTLDPTNIDLAKLAVIGNVGDMMARENC
                     GLVGPAREIVQDGVEYGNIIVQSRDLNCYGLSTRPVHICLGYCDEPYIDGISNNTNGA
                     LRFLERLGVELKNPRGGWLVWEELPFSDRRRIVSALAQHLIAHGQDTGRLLAETYIFP
                     DEPERTPLRNASEYATLLNACGRWAKPRVGSSICRGMRGEAYREAEYMLGHHRSVIRD
                     LLHYILDTGVTELSHLQYIHTGDRFPDTIVGIGAGMALSRLNWRKPIMVLSAMADEPD
                     LTKVSMRTNEWVLSRGVDLQQALVEASASVGGAGGGHRIAAGAFIPRETEEEFVDGVN
                     RVLKRQFAAAGQDDR"
BASE COUNT          879 a         1627 c         1630 g         1057 t
ORIGIN      
        1 ggctcgatgc aaaccgttca gccagagatt caggagagca accccagcgc cacccgctcc
       61 gccgaccact acccataata cccatcagtg caaacgcctc ttgatgagag tgatggtcgg
      121 ggggacgttt gaccccctcc atgccgggca ccggaaactc ctctcccgct cctttgacct
      181 tgccgggccc gccggcgagg ttgtgatcgg gctgacaacc gatgagttcg ccggcacgaa
      241 ggagcacccg gtccggccgt accgggaacg actgaacaac gtcacggcat tcatcctgga
      301 gaagggttac accgcctcct gggcggttga acctctctcc gaccgctatg ggagcgccgt
      361 cgatgctgat ttcgatatcc ttgtcgtctc cgaggagaca ttccatgttg ccctggatat
      421 caacgaactc cggcggcaga acggcctggc aaaggtcgac ctatatgttg tctcgtgcgt
      481 cctcgccgag gacgaacggc ggatctcgag cacccgtata taccggggcg agatcgaccc
      541 gcacgggcgc ctgatcagat gatgtggttt gctatattgc ccttgatcaa ccagtcgcag
      601 tagaggcagt gcagcccctt atcaagcacc tcaaaggtgc tctccaccgg ttcgtttgtg
      661 ttcgtgatgc agccggggtt cgggcacctg accacgccct tgatgacgcg ggggatctcc
      721 acacccttct tctccacgac ctcatactcc cggatgatgt tgatccttgc ctgcggcgca
      781 agcagggcga tccggtcgac ctcctccttg cggagttccc ggttctcgat cttcacgata
      841 tccttctttc caagccgcct gctctcgacg tttgtcgcga tgctcaggca ctcgtttgtc
      901 gaccccgtga tgccaaggat ccgcaggacg ttcagcgcct cgccggccgt gatatggtct
      961 atgacggtgc cgttccggat ggggctgacg agcagcccgt gtgcggtatc tttcctgctc
     1021 atctcatcac ctcgtgaagg agcgccatcc tgaccggaac accgttgcgc gcctgctcaa
     1081 agtagcgcgc atacggcgtc ctatccacag caggatcgat ctccccggcg cgcggcaggg
     1141 ggtggaggat catcaggtga tcccgcacac cctccagcag ttgtgtcgtt atcctgtaac
     1201 tcgaggctac gttgtagtat gacgccgtgt ccgggaaacg ttcgcgctgg atcctcgtga
     1261 catacagcac gtcgagttcc ctgatcgcct cctcaacgct cgcgtgctcg accacctcca
     1321 caccgcgctc ccgcagttca accgctatgt tcaccggcat ctcaagcccc gtaggcgcga
     1381 tcgtgtgcag ggtgacgccg tagagggaga gtgcaagcgc cagcgagtgc gccgtcctgc
     1441 cgtaacggag atccccgagg agccccgcat cgataccgtc aagcggcatg gattttcgga
     1501 tggtgtagag gtcaagcagc gtctggctcg ggtgctgccc cgcaccgtcg ccggcgttga
     1561 tcaccgggac ggaggagaac tcggctgcaa ggcgtgccgc cccctcttta gggtggcgga
     1621 gcacgatggc gtccacgtag ccgctcacca cccggatggt gtccgcgagc gtctccccct
     1681 tgacgatgga actcgcctcc acagaatcga cgctgatcga ctgcccgccg agccgggcca
     1741 gggcggccgc aaacgacatc cgtgtgcggg tgctgggttc aaagaagaga agcgccacga
     1801 gtctgtcctt gagcagcccc ggcgtgtaac gcccggtctc gaagacctct gccctatcta
     1861 gcaggtgatc gaggtcttcc ctttcaaaat cgcggataga gataatgtgg tacatcgtgc
     1921 ggctaccttc cggggctcgc tttcaacctt tccgccccat cctctcatgg atactggttc
     1981 accgcgcaga aagaaaatac cttttcgagt gatcggatga gaacctttct catccccggc
     2041 cgccccatac ttactcaacg gaggatagaa tggttccagg cgagaaagaa gggtatgccg
     2101 ccgagacctg ctcccattgc ggggggctcg gctgcaccta ctgcagcgga aagggcagcg
     2161 tgatggtgga gcagccatcg cgcaggtgca ggcactgcgg cggcgacggc tgcatctact
     2221 gcggctacac cggctgggag cacccgctga aagagtatta gcctctctac cggcgcgcga
     2281 tggtgatgta gccgctgtgg gcaacccggg tcgacggcct ggttcccctg gcggaacgcg
     2341 tcagttcacg ctcgatgcac tcgtagcagt ggacctcgcg aaagatcggt gccgcggcat
     2401 cgagcgcggt gaacgtatgc tcgaggaacg gggtgtagca cgccaggtag ccgccgggcc
     2461 tgaggagcga gaaggcgtgc tccacgtgtg ccggggttat cgtcatgtcc aggtggacca
     2521 catcgaactc tccggttgcc tggagcatat cgcccgccat cacctcgacg ttatcgagcc
     2581 tggcgtttct gatgttcttc tccgcaagcg ctgcaaactc cgggcgcacc tcgcaggtct
     2641 tcacccgccg ggcaatcttt ccaaagtaga tcgcggcgac accgctcccc gtccccgcgt
     2701 cgaggacggt gtcgtcccgg ttcatcccgg tgtaggctat aaccagccct atgtcttttg
     2761 gaagcatggg agcgccgctg cgctttgcgt ggacaaaaaa atcggttggc cgcgggcgca
     2821 gcacgatgaa cgggacgccg aggtgtgtcc tgacctcgcc gccggggttc atcccgggga
     2881 gcgccgagag gtcgatcgtc ccgcggtcgg tggagaactg cccctccccc gccgtcacga
     2941 agtactcccg gtcctctccg acgagcagga ggcgttcgcc ggtctcgatc attgctcggc
     3001 gagtttcagt atggcttcgg cgatatcgcc gtgggtctcg atgagcgcgg ccagcgcggc
     3061 ctcttctgtt gctccggtct gggatgctac gagcgcgacg tcctcgtccg ggatctcagg
     3121 ggcggcctcc tcgaaccggg gttcgccggt gatctggtag gtggttaacc cctgcatcct
     3181 ggtcgcgacg acctgggcct cgtcaaagat gtagtccccg gcggacgtct ggatgatgac
     3241 cctctcaacg ccctcgagtt cctccatctc catgcccatc tgcttcatca tctgcttcat
     3301 ctttttaggg tttatctttc ctggatacat tcggatcctc tcccctgcct tatttttacg
     3361 gctacaccgt aattaaatgc cagcatctcc cggccggaga ggatggcggc gccggtggca
     3421 atgaggttat catccccggt caccaccagg acctcgtccc ctgcgcggat gccttcgtcg
     3481 gcagagacca cgtgttttgc catagcgttc ctgccgtcgg cgatgaacgc cgctacatcc
     3541 tcctgcaccg ccaccctgta gcccggcggc ggtatgtggg cggcaagacg aaccgctccg
     3601 gcgatcccga gggtgaggcg gccgtcctgc gcacgaacgg tcgcaagcct ctcgccgtcg
     3661 agacgcacct ggcggatacg ccctgtcgtg gataactgga acgtgcatcc gtcggggaat
     3721 atggcttccc ctgcccctct gccgaactgg aagtcaccta tcgtcctgac ccgctgcagc
     3781 aaactgtctt ttgagtaccc ggttgacgcc atcaacaaac tcctcttctg tttcgcgggg
     3841 gatgaacgcc ccggcggcga tacggtgccc gccgccggcg ccgcccaccg atgccgaggc
     3901 ctcgacgagc gcctgctgga ggtcgacccc gcggctgagc acccattcgt tcgtccgcat
     3961 cgagaccttg gtgaggtctg gttcgtccgc cattgcgctg agaaccatga taggtttacg
     4021 ccagttcagt cttgagagag ccatgcccgc tcctatcccg acgatggtgt cggggaaccg
     4081 gtcgccggtg tggatgtact ggaggtggga tagttcggtg acgcctgtgt caaggatgta
     4141 gtggagcagg tcgcgtatga cggagcggtg gtggccgagc atgtactccg cctcgcggta
     4201 ggcctcgccg cgcatacccc ggcagatgct gctcccaacc cgtggtttcg cccagcggcc
     4261 gcaggcgttg aggagggtgg catactctga ggcgttccgg agcggtgtgc gttccggttc
     4321 gtccgggaat atgtatgtct cggcgaggag gcggccggta tcctggccgt gggcgatgag
     4381 gtgctgggcc agggcgctga cgatcctgcg ccggtcgctg aacgggagtt cttcccagac
     4441 gagccagccg ccgcgggggt tcttcagttc gacgccgagc cgttcgagga accggagcgc
     4501 gccgtttgtg ttgttggaga tcccgtcgat gtagggttcg tcgcagtagc caagacagat
     4561 gtggacgggg cgggtggaga ggccgtagca gttgaggtcg cggctctgga cgatgatgtt
     4621 gccatactcg acgccgtcct ggacgatctc gcgggcgggg ccgaccaggc cgcagttttc
     4681 gcgggccatc atgtcgccga cgttcccgat cacggcgagt ttggcaaggt ctatgtttgt
     4741 cgggtcaagc gtctttgcga ccaggtaggc gaggccggcg gcgctcatcc gggtgatgcc
     4801 gtggtcgagg cagttgatca tggggtaggc cgttgtgcag ggctggctga cgtggtgatc
     4861 caggatcagg acgtcctcgg cggtgagacc gcggctctcg aggagggcct gctgcccggc
     4921 gcccaggtct gcaaatagtt tgagggagtc gtcgtccggg atgtgccgca tcgccatcgg
     4981 ttcgagctgg cgaacgaaga ccgattcgac cggtatgccc tcgcggctga gcgcctgcgc
     5041 caggatcgcc tccgtgctga tgccgtcggc gtcgatgtgg gagacgattg tcaccgagtc
     5101 agcgtcagat atgatctccg ccgctctccg cagatcgcgc tcgaggttca tcttccatat
     5161 ctatgggtgg tgcagccact taacttgtgg ggg
//
LOCUS       DAOSZX010000098         5181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13389_length_5181_cov_20.595981, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000098 DAOSZX010000000
VERSION     DAOSZX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13389_length_5181_cov_20.595981"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1684
                     /locus_tag="PK196_07215"
     CDS             167..1684
                     /locus_tag="PK196_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON41241.1"
                     /translation="MNKYSFIARMPDRPGALHRAAEIIKSYSGNIIRVQYDRRIDPAT
                     VFFEVAAAPEGYARMKEDLHAIGYLQESLPTPGFLKFSVYLPHEPGSLFDLLTYITGA
                     GANIAHIDFDERRQDPGRVTISLSVEESEVVDSLLDRLKSRYRLEILEYDTTGEKLDD
                     TVFYVRFAQALRGLIGSAEDEFLLSLLQDINHIVQELNNLGQDPKEVFDCILRTGKTL
                     RETTGDRFYADVQRIPLSDAVEVLCFQMPGGGNIFMLNAPEESVMIDTGYGIYHQDAL
                     RMFQHYGIGDRGRIRRVYITHADADHCGAAGFFDAKHFTHPWSVEIIRRANRAYGSRS
                     ESSILEEVYTTVINLFSHFTPPENPETFPTERLASRSIFPVIARFAVHDLEFEVLESL
                     GGHMHGQVYFLCPTHGIIFTSDTLINFGSLDDDRKRYNSLADFLVTSVNVDSERARQE
                     RTALLGLIRDLDEELAPTGRRCLVAGGHGAVSVLSPEGRLEAVGPIERYPPGEAR"
     gene            complement(1689..3002)
                     /locus_tag="PK196_07220"
     CDS             complement(1689..3002)
                     /locus_tag="PK196_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HON41242.1"
                     /translation="MADGERDASYPGTNLRRELGLAAVVLSGVGIILGAGIYALLGEA
                     AGMAGNAVWLTFLVAAAVAGLSALSYAELSSIYPKASAEFEYVSRAFGGRLAFVVGWL
                     IILSGILGAATVALGFAGYFSDLVGLPLLPSAVMIILILSGILIAGIRETAGVAISMT
                     LIEVAGIVMVILIGLPYLGRVDYLEMPLGIPGLLQASALVFFAYMGFEEMVKLSEETR
                     NPERTIPVALLIALAISTLLYVLVSVAAVSVVGWEQLSTSRAPFAEVAAVALGPTAFT
                     LISVIALFATANTALMMMLASSRIIYGMASSFSLPPSLARVHPRTQTPWTATIVVAIA
                     TIAFLFAGEIDFVANVTNFTLFATFIVINASVILLRYRAPDLPRPFRIPGSIGSLPVL
                     PVIGIASTLILLAHLEPLVLLLGGFMVVLAAAIALLWEERWRGAA"
     gene            3210..4982
                     /locus_tag="PK196_07225"
     CDS             3210..4982
                     /locus_tag="PK196_07225"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HON41243.1"
                     /translation="MNFLITSGRTIAQGNELYYKDRENYSVETGRCFVNPFDLLELDA
                     VAGDSILLSTGTGEEVFTATPCEDVASGTIFLPCGPHANAILHAATRGTGAPDYKWIE
                     GSARRVETLPRSGREILAEEGGAPLPLPEVQSGERAGEAVAIHDVVCPLCGCLCDDLV
                     VRVEGGGVTSVDNACSLGAAKFLSQSRLRKPLVRDGGSWSETSYEEAIRSAADILAEA
                     ERPLLFGWSGTSAEAQCIGIHIAEAIGGVIDNCSSICHGPSLLGIQEAGHPGCTLGTV
                     KNRADLVIYWGSNPIESHPRHLSRYTTFVNGRYREEGHHDRTLVVVDIRKTDTAKIAD
                     EFIQIRPGGDYAAFAALRAVLRGHAAILPATVAGIRRERLIWLADLCRNAQFGSLFTG
                     IGLTQSPGRYKNVRSAIQFVDELNRYTKFTLTPMRGHWNVNGTNQTFSYLTGYPYGVD
                     FSRGIPCYNPGETTAVDVLSRGEVDACLVIGADPGAHLPRRCNEHLAKIPTIVIDPFV
                     SLTSSFATLHIPVACVGIDCEGTGYRMDSVPIRLRKVLDLGLPTDEEVLSALLERILE
                     RGQAGSAVIDERGAATTTRAPVIM"
BASE COUNT         1111 a         1548 c         1589 g          933 t
ORIGIN      
        1 cacggagagg tttatcaaac cagtgaggca ttggtttgat ggcctcctgg agagttgctc
       61 actctcattg ttcattttat ctccagggcg ccattcgtgc cgtaaccgcc cgaccgaatg
      121 gtattaaggc agattcttac ctataaacaa tccgcaggtg aaccgggtga ataaatactc
      181 tttcatagcc aggatgcccg accggcccgg ggcgctgcac cgcgcggccg agatcatcaa
      241 atcttactcg ggaaacatca tccgcgtgca gtatgaccgc cggatcgacc ccgccacggt
      301 cttcttcgag gtggctgcag caccagaggg ctacgccagg atgaaggaag acctccacgc
      361 gatcggctac ctccaggaat cgctgccgac accagggttc ctcaagttct ccgtatacct
      421 cccgcacgaa cccggctccc tctttgacct cctcacctac atcaccgggg cgggggcaaa
      481 catcgcccac atcgactttg acgagcggcg gcaagacccc ggcagggtca ccatcagttt
      541 gagcgtcgag gagagcgagg tcgtcgatag cctgctcgac cggctgaagt cacgctaccg
      601 cctggagatc ctggagtacg acacaaccgg cgagaaactc gacgacaccg tcttttacgt
      661 caggtttgcc caggcgctgc gggggctgat agggtcggcc gaggacgagt tcctcctctc
      721 actcctccag gacatcaatc acatcgtcca ggaactcaac aacctgggcc aggacccaaa
      781 agaggtcttc gactgcatcc tgcggaccgg aaagacgctc cgtgagacca caggcgaccg
      841 gttctacgcg gacgtccagc gcatcccgct gagcgatgcg gttgaagtcc tctgtttcca
      901 gatgcccggc ggcgggaaca tcttcatgct caacgccccg gaggagagcg tcatgatcga
      961 taccggttac ggtatatacc accaggacgc cctccggatg ttccagcact acggtatcgg
     1021 tgaccggggg cggatccggc gggtctacat cacccacgcc gacgccgacc actgcggggc
     1081 ggcaggattc ttcgatgcga aacactttac ccacccctgg tcggttgaga tcatccggcg
     1141 cgcgaaccgc gcctacgggt cgcgcagcga gtcctcgatc ctggaagagg tctacacgac
     1201 ggtcataaac ctcttctccc acttcacccc gcccgaaaac ccggagacct tcccgacgga
     1261 gaggcttgcc agccgctcga tatttcccgt catagcgcgg tttgctgtcc atgacctgga
     1321 gtttgaggtc ctcgaatccc ttggcgggca catgcacggc caggtctact tcctctgccc
     1381 cacccacggt ataatcttca cctccgatac cctgatcaac tttggaagcc tggatgacga
     1441 caggaaacgc tacaactccc tggccgactt cctggtgacg tcggtcaacg tcgacagcga
     1501 acgcgcacgc caggagcgca cggcgctgct cggactgatc cgggacctgg atgaagaact
     1561 tgccccgacc ggccgcaggt gcctggtcgc gggcggacac ggcgctgtat ctgtcctctc
     1621 cccggaaggg aggctggagg cggtcgggcc gattgagcgt tacccgccag gagaggcgcg
     1681 gtagggcatc acgccgcccc gcgccaccgc tcttcccaga gaagcgcgat cgcggccgcg
     1741 agaaccacca tgaacccgcc gagcagcagc accaggggct ccaggtgggc aagaaggatg
     1801 agtgtcgagg ctatcccgat gacgggcagc accgggaggg agccgatgct cccgggtatc
     1861 cggaacggcc gtgggagatc cggcgcacgg tagcgcagca ggatcacgga ggcgttgatg
     1921 acgatgaaag tcgcaaagag ggtgaagttg gtgacgtttg cgacaaagtc gatctcgccg
     1981 gcgaagagga aggcgatggt ggcgatcgca acgacgattg ttgccgtcca gggcgtctgc
     2041 gtccggggat gaacccgggc aagagatgga ggaagcgaga aggaggatgc catgccgtag
     2101 atgatcctcg atgatgccag catcatcatg agggcggtgt ttgcggtggc aaagagcgct
     2161 attacggaga tcagggtgaa ggccgtgggc ccgagcgcga cggcagcaac ctcggcaaac
     2221 ggcgccctgg aagtggagag ttgttcccag ccaacgacag agaccgctgc aaccgagaca
     2281 aggacgtaga ggagggtgga tatggcaagc gcgatcagca gagcgaccgg gatagtccgt
     2341 tccgggtttc tggtctcctc cgagagtttc accatctctt caaaccccat gtaggcaaag
     2401 aagacaagcg cagacgcctg gagtagaccg ggtataccaa gcggcatctc aaggtaatcc
     2461 acccgtccga ggtatggaag gccgatcagg atcaccatga ctatcccggc gacctcgatc
     2521 agggtcatgc tgatggcaac ccccgctgtc tccctgatcc cggcgatgag gatgccggaa
     2581 aggatcagga tgatcatgac ggcggacggg aggagcggca gacccacaag gtcggagaag
     2641 tagccggcaa acccgagcgc gacggttgca gcgccaagga tgccggataa gattatcagc
     2701 cagcccacaa cgaacgcaag ccttcccccg aacgccctgg agacgtactc gaactcggca
     2761 ctcgccttcg ggtagatcga tgagagttcg gcgtaactca gggcgctcag gccggcgacc
     2821 gcggctgcaa cgagaaaggt cagccagacg gcgttgccgg ccatcccggc cgcctcgccg
     2881 aggagggcgt atatccctgc cccgagtatg atcccgactc cggagaggac gacggcggcc
     2941 agtccgagct cgcgccggag gtttgtgccg ggatacgaag cgtcacgctc cccatctgcc
     3001 atacgatatc actctgtctc tgctgcattt atggttgagc cttcaggatg ccggaaaccg
     3061 gttgctccgg gtgatgtctt ttgaagaggt ggatgagacg gcggcagcgt aacaggtgat
     3121 gtgcgagtac actatttttg gtttacttct gttgtatgta aatatcagat ccataacagt
     3181 tatcgtcgtt gactccagat agacctccga tgaactttct catcacgagc gggaggacga
     3241 ttgcccaggg aaacgaactc tactacaaag atagagagaa ctactcggtt gaaacggggc
     3301 gctgttttgt caacccgttc gatctccttg aactcgatgc tgtggcggga gactccatcc
     3361 tcctctcgac cgggacgggg gaggaggtct tcaccgcaac accctgtgag gacgtcgcat
     3421 cgggaacgat ctttctcccc tgcggaccgc acgcaaatgc aatcctgcac gcagccaccc
     3481 gcggaaccgg tgcaccggac tacaagtgga ttgaggggtc ggcaagaagg gtggagaccc
     3541 tgccgaggtc tggccgggag atcctggcag aggagggcgg ggcccccctc cctctgccag
     3601 aggtgcaaag cggggagagg gccggtgagg ctgtagccat ccatgacgtt gtctgcccgc
     3661 tctgcggctg tctctgtgac gatcttgtcg tcagggtcga gggcgggggt gtgacgtcgg
     3721 tcgataacgc ctgttcgctc ggcgcagcaa agttcctctc gcagagcagg ctcaggaaac
     3781 cgcttgtgag ggacggcggg tcatggagtg agacctccta cgaggaggcg atccggtcag
     3841 cagcagacat cctggccgag gctgaacggc cgctcctctt tgggtggagc gggacatccg
     3901 cagaggcgca gtgcatcggc atccacatcg ccgaggcgat cgggggcgtc atagacaact
     3961 gttcgtcgat ctgccacggc ccgtcgctcc tcggcataca ggaggcggga cacccgggat
     4021 gcacgctcgg cacggtgaag aaccgcgccg atcttgtgat ctactggggc tcaaacccta
     4081 tcgagtcgca cccccgtcac ctctcccgct acaccacctt cgtgaacggg aggtaccgcg
     4141 aggaagggca ccatgaccgg acgcttgtcg tcgtggatat aagaaagacc gatacggcaa
     4201 agattgccga cgagttcata cagatcaggc ccggcgggga ctatgctgcc ttcgcggctc
     4261 tccgcgcggt tctccggggg catgcggcta ttcttcctgc aaccgtcgca ggcatccgcc
     4321 gtgagaggtt gatctggctt gcagacctct gcaggaacgc tcaattcggg tcgctcttca
     4381 ccgggatagg tctgacgcag tcgcccggta gatacaaaaa cgtcaggagc gccatccagt
     4441 tcgtcgacga actcaaccgc tacacaaagt tcacgctgac accgatgagg gggcactgga
     4501 acgtcaatgg gaccaaccag acgttctcat acctgaccgg ctatccctat ggtgttgatt
     4561 tctcgcgcgg cataccctgc tacaaccctg gggagaccac ggccgttgac gtcctgagcc
     4621 ggggcgaggt cgatgcgtgc ctggtcatcg gcgccgatcc cggagcgcac ctccccagga
     4681 gatgcaacga gcatctcgca aagatcccga ccattgtcat agacccgttt gtctcactaa
     4741 cctcgtcgtt tgccaccctt catatcccgg ttgcctgcgt cgggatagac tgcgagggga
     4801 caggctaccg gatggactcc gtcccgatcc ggttgaggaa ggtcctggac ctgggcctcc
     4861 cgaccgacga ggaggtgctc tcagcgctgc ttgagcgtat actagagagg gggcaggccg
     4921 gcagtgcggt catagatgag aggggggctg caacgacaac cagggctcct gtgattatgt
     4981 aaaccagatc agatcgacca atagcaaaag ttaaatcctt ataaataaaa ataagtatgt
     5041 gccacgatag tgggtacata cttgagatcc aattcgatct gaaaacctca gcactatcat
     5101 cacccggcca ggaggaatgg agcgcccggg catcgtgccg gatgcacggg gatggggtgg
     5161 tacatgcctc cgctctgacc c
//
LOCUS       DAOSZX010000099         5142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13498_length_5142_cov_16.068803, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000099 DAOSZX010000000
VERSION     DAOSZX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13498_length_5142_cov_16.068803"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..3751
                     /locus_tag="PK196_07230"
     CDS             254..3751
                     /locus_tag="PK196_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HON41244.1"
                     /translation="MALFDRLRTDVEGLRQAKDYPGLIALLDGDDPAGRTEAARALCS
                     LGIGAIPNLLRSLERARPASQTRMLSALVSVGTPSIPLLLALILRADPALQTAIASAI
                     ASAGEPMFEALLPALQHNQPAIRRATVIALQGAGKKAIPHLTPALRDSNPAVRKEAAR
                     VLAGLRWTPTETSEQVEFYYLLEDWGELAKLQGAAVPILLQALESKDPRIRSESAGAL
                     GKIRDFRAFPALAKAAKDRNENVRMRAVEALGYIGDPQAMPILVDALNDPRHEVRMEA
                     AWALDRLGWVPQSNLQRVDYLIAKGRWSELATMGRTAIPSLIRALKIDYSGVRIGATE
                     ALRRMGQPAISALKTEASSKDLGRRERAKTALRYIQQRQEEETLTRPVEEDPSRYERE
                     LKEGLAIQKRFEKLFGRPRYTPVTEGSGKPQKREAEPPKQESPAAPAPVNPKKEMNLD
                     EIIRESRIAEEAWARVKERWKADRSAPEGAIPLEHLIPHDFEEAIVRMDEAGGEEGGS
                     VLLSDEEDREPQPLEIPKIAPETPEEPKPPEPAAHKSPLERYLEALRSPDEAVRAAAV
                     AALQSMGKEAVIYLIQVLNDPYPAVRVAAAEALGQIGDADAVDPLIQATHDEREDVRI
                     AAASALARIGDRKSIQPLIRLFADRYHGVRVAAADAVAVFGRAALEALEEALSDPVTV
                     VRVTAARAIGLIGVIESIPVLIDHLGDPAPEVRWSVARALGEFGPPVIDPLSIVMSRG
                     SRDMRLAAIDALWEIEDERAVELLRRALDDEDEEVREKAAAALKKRQVIDVWRSALGS
                     QVLEEVQAPKKKKKPIRLEDRKAFERAGPQEVGKLISALKEKDWQMQLNVATRLIMMG
                     KPAVDGLIRALRTEDPEIQAAAAGILGEMRETAVEPLMDALDDSDRFVRIVAAQNLGK
                     IGNKRAIEALIGALHRETDPEVRTAVAEGLGYMGSRQAIEPLTLALQDRDERVQVAAA
                     RSLGYIGDLRALEPLIHALRDVDDRVRHAALEALKYPDETMRSSLIGALRSGDEDFRA
                     GVAEALEAGGWVPETTEQRVLYLMAMNRWSEVEEIGADALPVLAEALDDPLVEVRANA
                     VRAINRIGGEESVPLLIHALRDETFMVRKRAERGLVEMGEAALPGLEEALEETEDEER
                     ETLQRIIDEIRARNVVEEA"
     gene            complement(3748..4422)
                     /locus_tag="PK196_07235"
     CDS             complement(3748..4422)
                     /locus_tag="PK196_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HON41245.1"
                     /translation="MKIIGINGSPRGPESRTRKLVQAVLDGAKSAGADVELVDVSRLQ
                     IEYCNGCHICYEQGKCINDDDFTELYESMLESDGIVLGSPNYVNSVTAQMKTMLDRIT
                     DAIHCQMFLGKYGCAVSTAGGSGADEVAAYLNRVLLSLGANTVGGVDAVVGTETDPAA
                     MEAAEKKAYDLGRRLVQAIKNRETYPEQEKIHADMIEHMVALVTANKDRWPHQYSHWK
                     EAGRIP"
     gene            complement(4489..4953)
                     /locus_tag="PK196_07240"
     CDS             complement(4489..4953)
                     /locus_tag="PK196_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HON41246.1"
                     /translation="MGGLSIPVKSLMSLLLLGCLLWAAVSAGCMETSPTPATPTPTPA
                     PASTYTTYSPVGPKPAFSSSITSLQKRMRSDGSCYWTVAGSVTNEGDGTARNAAIRFM
                     LIDDEASMIRATETVFVPRFPAGETKAFTVDALPGECDRQYHAEIEATCDIP"
BASE COUNT          981 a         1589 c         1677 g          895 t
ORIGIN      
        1 ccccccgaag aatcctgtct ctgtcccctg gcacggccac cgccggccgg gtggcggggc
       61 gggcagccgc aaaagaggtc tcagcgccgt gtgatctggg gcattacacc atgagcgacc
      121 tggctttatg ggctttcata cccgctcctc tcggagaacc gccgaaccgg tctctcacag
      181 gaggcggcat agtagtttta ttggttgcgg tgacgaacat tcctccgaac aaacgaatat
      241 ccagccagat gtcatggcac tctttgacag gttaaggaca gatgttgaag gtctgcgtca
      301 ggcgaaggat taccccggtc tgattgcact cctcgacggc gatgaccccg ccggccgcac
      361 cgaggcagca cgcgctctct gcagcctggg catcggcgcc atcccaaacc tcctccggag
      421 cctggagaga gcccgccccg cctcacagac ccggatgctc agcgcgctcg tctcggtcgg
      481 cacaccctcc atccccctcc tccttgccct catcctccgc gccgaccccg cgctccagac
      541 cgcaatcgcc agtgccatcg catctgcggg ggaaccgatg tttgaggcct tgttgcccgc
      601 cctccagcac aatcagccgg cgatccggcg tgcaacggtg atcgctcttc agggagcggg
      661 caaaaaggca atcccccacc tcaccccggc gctccgcgac agcaaccccg ccgtgcggaa
      721 agaggccgcc cgcgtgcttg ccgggctgcg gtggacgccc acagagacct ccgagcaggt
      781 ggagttctac tacctccttg aagactgggg agaactcgca aaactccagg gagcggccgt
      841 tccgatcctc cttcaggccc tcgagagcaa agacccgcgt atacgctctg agtcggcagg
      901 ggcactcggc aagatccgcg atttccgggc atttcctgcc cttgccaaag cagcgaagga
      961 ccgcaacgag aacgtccgca tgcgggccgt cgaggcgctc ggctatatag gcgatcccca
     1021 ggcgatgccc atcctggtcg acgcgctcaa cgacccccgg cacgaggtgc ggatggaggc
     1081 ggcgtgggcg cttgaccgtc tgggctgggt gccgcagagc aacctccagc gggtggacta
     1141 cctgatcgcc aaagggcgct ggagcgagct tgccaccatg gggagaaccg ccatcccctc
     1201 cctcatccgg gcgctcaaga tcgattactc gggcgtgcgc atcggtgcga cggaagcgct
     1261 gcgcaggatg gggcagcccg cgataagcgc cctgaagacg gaagcctcct caaaagatct
     1321 cggccgccga gagcgggcaa agaccgctct ccgctacatc cagcagcgcc aggaagagga
     1381 gacccttacc cggccggtcg aggaagaccc ctcccgctac gagcgtgaac tcaaagaagg
     1441 gcttgccatc cagaaacggt ttgagaagct gttcgggcgg ccaagataca cccccgtcac
     1501 agagggttcc ggaaaaccac agaaacgtga ggcagaaccc cccaaacagg agagtcccgc
     1561 ggcccccgcc ccggtgaacc ccaaaaaaga gatgaacctt gatgagataa tcagggagag
     1621 caggatagca gaagaggcgt gggcacgggt caaagagcgg tggaaggccg ataggtcggc
     1681 ccctgaaggc gccatcccgc tggaacacct catcccccac gatttcgagg aggcgattgt
     1741 caggatggac gaggccgggg gcgaggaggg tgggagcgtt ctcctctccg atgaggaaga
     1801 ccgtgaaccc cagcccctcg agatccccaa gatcgctcca gagacacctg aggaacccaa
     1861 accccccgaa ccagccgccc ataagtcgcc gctcgaacgg tatcttgaag ccctccggag
     1921 ccctgacgag gccgtcaggg cagcggcggt cgcagcgctg cagagtatgg gcaaagaggc
     1981 ggtcatatac ctcatccagg tgctcaacga cccgtatcct gcggtacgcg tagccgccgc
     2041 ggaggcgctc ggccagatcg gggatgcgga tgccgtcgac ccgctgatcc aggccaccca
     2101 cgacgagaga gaggacgtgc ggatcgcggc agcctctgcc ctcgcgcgta tcggcgaccg
     2161 gaagtcgatc cagccgctga tccgcctctt tgccgaccgc taccacggtg taagggtcgc
     2221 cgcggcggac gcggtcgcgg ttttcgggcg tgccgcgctc gaagcgctcg aggaggctct
     2281 ctccgatccc gtgaccgtcg tccgggtgac ggcggcgcgg gctatcgggc tgatcggagt
     2341 gatagagtct atcccggtct tgatagatca cctgggcgac ccggcgccgg aggtcaggtg
     2401 gagcgttgcc cgtgccctcg gcgagtttgg gccgccggtt atcgatcctc tctccattgt
     2461 gatgagcagg gggagcaggg atatgcgtct tgcggcgatc gatgccctct gggagatcga
     2521 ggatgagcgt gcggtcgaac tcctgcgccg ggcactcgat gatgaggatg aagaggtgag
     2581 ggagaaggcg gcagccgccc tcaagaaacg gcaggtgatc gatgtctggc ggagcgcgct
     2641 cggcagccag gttctggagg aggtgcaggc tccaaagaag aagaaaaaac cgatccggct
     2701 ggaggacagg aaagcgtttg agcgtgccgg gcctcaggag gtcgggaagc tcatctcggc
     2761 gctcaaggag aaggactggc agatgcagct caacgttgcc acgcgtctta tcatgatggg
     2821 aaaacctgct gttgacggcc tcatccgggc tctcaggacc gaggacccgg agatccaggc
     2881 cgccgctgca ggtatactcg gggagatgcg cgagaccgcg gtcgaaccgc tcatggacgc
     2941 cctggacgac agcgaccgct ttgtccggat cgttgccgcc cagaacctgg gcaagatcgg
     3001 gaacaaacgt gccatcgagg ccctgatcgg cgccctgcac agggagaccg atcctgaggt
     3061 caggacagcg gtggcggagg gcctgggcta catggggagc agacaggcta tagagccgct
     3121 cacacttgcg ctccaggacc gtgacgaacg cgtccaggtc gccgccgcga ggtcgcttgg
     3181 ctacatcggc gacctccgtg ccctggagcc gctcatccat gcgctccgtg atgttgatga
     3241 ccgggtcagg catgccgcgc tcgaagcgct aaagtatccc gatgagacga tgcgcagcag
     3301 cctgatcggt gccctccggt cgggtgacga ggacttcagg gcaggggttg ccgaggcgct
     3361 ggaggcgggc ggctgggtgc cggagacaac agagcagcgc gttctctacc tgatggccat
     3421 gaaccgctgg agcgaggtgg aggagatcgg tgccgatgcg ctcccggtcc tcgcggaggc
     3481 gctcgatgac ccgctggttg aggtcagggc aaacgcggtt cgggccataa acaggatcgg
     3541 gggggaggag tctgtccccc tcctcatcca cgcgctccgg gacgagacgt tcatggtcag
     3601 gaaacgggca gaacgcggtc ttgtcgagat gggagaggcg gcactgcctg gacttgaaga
     3661 ggcgctcgag gaaacggagg atgaggagcg ggagacgctg cagcggatca tcgatgagat
     3721 ccgcgctaga aatgtggtgg aggaggctta gggtatccgc cccgcctctt tccagtggct
     3781 gtactggtgg ggccagcggt ccttgttcgc cgtgacgagc gccaccatgt gctcgatcat
     3841 atcggcatgg atcttctcct gttcagggta ggtctcgcgg ttcttgatgg cctgcaccag
     3901 tctcctgccg aggtcgtaag ccttcttctc ggcggcctcc atcgctgccg ggtcggtctc
     3961 tgtgccgacg acggcgtcca cgccgccaac ggtgtttgcg ccaaggctca gcaggacgcg
     4021 gttgaggtat gccgcgacct catccgcccc tgaaccgccg gcggtggaga cggcgcagcc
     4081 gtacttcccg aggaacatct ggcagtggat ggcatccgtt atccggtcga gcatggtctt
     4141 catctgggcg gtgacagagt ttacgtagtt gggcgagccc aggacgattc cgtcgctctc
     4201 gagcatactc tcgtagagtt cggtgaaatc gtcgtcgttt atgcacttcc cctgttcgta
     4261 gcagatgtgg cagccgttgc agtactcgat ctggagtctg gagacgtcca cgagttccac
     4321 atccgccccg gcgctctttg ccccgtccag gacagcctgg acgagttttc gggtcctgct
     4381 ctccggaccc ctggggcttc cgttaattcc tattatcttc ataacatctc acctctttgc
     4441 aacatccatg cgcccgccac cccggctgcg cgccagacag cacaggcctt acgggatgtc
     4501 gcaggtggcc tcgatctcgg cgtggtattg ccggtcacac tcgccgggga gcgcatcgac
     4561 cgtgaatgcc ttcgtctcgc ctgccgggaa ccgggggacg aaaacggtct cggtcgcgcg
     4621 gatcatgcta gcctcgtcgt cgatcagcat gaacctgatg gcagcgtttc ttgcagttcc
     4681 atccccctcg ttggtcacgc ttccggcaac cgtccagtag caggatccgt cgctgcgcat
     4741 gcgtttctgg agcgaggtga tgctggacga gaatgcgggt ttcggcccaa cgggtgagta
     4801 ggtggtgtag gtcgaggccg gtgcaggtgt cggggtcggc gtcgccggcg tgggggaggt
     4861 ctccatgcag cctgcagata cagcagccca gagaaggcac cccaggagca gaaggctcat
     4921 gaggctcttc accgggattg agaggcctcc catgatgaag catttcggcc ggggaagtat
     4981 ataagggata tggagacggg agcaggactt ggatagcgtt ataacctctt cctggatgga
     5041 ttccggtgga tctccatcgc tgcaggccgc tctccaccta tgatggtgga ggccatcaaa
     5101 tcaacggttc tgtgatataa actccccgtt tcttcccggt at
//
LOCUS       DAOSZX010000100         5141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13504_length_5141_cov_24.685804, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000100 DAOSZX010000000
VERSION     DAOSZX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13504_length_5141_cov_24.685804"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PK196_07245"
     CDS             complement(<1..1529)
                     /locus_tag="PK196_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON41247.1"
                     /translation="MEIADLPLPAQLAASYTDHGIRELYPPQAACVEAGLFSGKNLLI
                     AIPTASGKTLVAEMAMHHQVANGGKCLYIVPLRALASEKFEEFSGKGLRVGIATGDFD
                     RRDEYLGRNDIIVATSEKVDSLLRNRTPWLSEISLLVIDEVHLIDDPSRGATLEMVIT
                     KLRHKNEGVQIIALSATIGNPGDLAGWLDAELVESDWRPVELREGVFLLDTIHFTDSI
                     RQIERRSKHEDLDLVLDTVAEGGQCLVFVSSRKNAEAFAKRAASGLKLSNPILAGYAD
                     RIRSNAATDMGKTLAACVAAGAAFHHAGLPREERQIVEQGFREGQIKVIASTPTLAAG
                     LNLPARRVIVRDYMRFNAGEGMVPIPVREYRQMAGRAGRPHLDPYGEAVLIAKSDDMV
                     DELFDCYIEAPPEDVRSRCANEAILCTHILSLIATGFARERDEVMGFMDGTFYAHQGE
                     NPLALKRVVDRVLDFLLEAEMITDVGGWLDTTDYGSLVSRLYIDPRSAELIVTAMAGQ
                     K"
     gene            complement(1614..>2021)
                     /locus_tag="PK196_07250"
     CDS             complement(1614..>2021)
                     /locus_tag="PK196_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HON41248.1"
                     /translation="IEGDDPVGVVTAANVVSAIGRGFSPERAFRLLDEEDMILVLIDL
                     TDLAGTTRHLERLRGRIIGKSGTSRAQIEDMTGTEISVYGKTVAIIGLPEQVEIARKA
                     VEMLIQGVPHETVYAFLDRKKKEARQEMLEYYS"
     assembly_gap    2022..2121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2122..2781)
                     /locus_tag="PK196_07255"
     CDS             complement(<2122..2781)
                     /locus_tag="PK196_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HON41249.1"
                     /translation="MSRNEEYERFDREIAEMGVKIKDADSLKVREDVFDEVTLIALYK
                     LVHRKLISSIGGPISTGKEANVFYGERGAQGIAIKIYRIQTGNFKAMTDYLAGDRRFS
                     SVRGTRKGIIFAWTKKEFSNLARAHEAGVPTPEPLAFDRNILLMEFLGEDEIPYPQLR
                     NAEVEDYGAVYNEILAYVDLLYREARLVHADLSEYNILYHEKPYLIDMGQAVTLDHPQ
                     AR"
     gene            complement(2789..3736)
                     /locus_tag="PK196_07260"
     CDS             complement(2789..3736)
                     /locus_tag="PK196_07260"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HON41250.1"
                     /translation="MDPYKLATRNTVEVVTGEELRALLDRPVRRVYTGYEPSGEIHLG
                     HMVTVNKLMDLQQAGFEVTVLIADLHAFLNRKGTMEEIREIAEYNRRCFEGLGLSGAR
                     YVLGSEVQLTSDYELAVLELSQRVTLNRAKRSMDEVGRQMENPMVSQMIYPIMQMVDI
                     ATLEVDAAVGGIDQRKIHMLAREHLPSIGYPAPVCIHTPIINGLDGKKMSSSAGNVIS
                     VADSDEEIQRKIKRAFCPPEIENNPVLEILRYHVFPRSGKITIRRPAKFGGDREFYTY
                     EDLERAYAAGEIHPMDLKAATASHLIDILAPVHDYVFSR"
     gene            complement(3793..4818)
                     /locus_tag="PK196_07265"
     CDS             complement(3793..4818)
                     /locus_tag="PK196_07265"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HON41251.1"
                     /translation="MIKVAINGYGTIGKRVADAVAAQPDMEVIGVSKTSVSAEAYIAK
                     ERGYPLYIADISRRPAFEKAGLEVAGDVETMIRAADIVVDATPGGVGEKNRPIYERLG
                     KKAIFQGGEEHEVAGFSFNAHANYREAEGKQFARVVSCNSTGLIRLIHALDQAFGVER
                     VMAVMVRRAADPDDIKRGPVDAIVLNPATIPSHHGPDVQTVLPHIEIVTLAMIVPTTF
                     MHMHTVQMRLKSKTTREEVLKVFERHNRIGLIRKATGIVSNAQLREYTQDIGRPRTDL
                     WENGVFEESVAVLNGKDFYCFQAIHQEADVIPENIDCIRAMLGTVKDPEESIRMTNEA
                     LGLVPIG"
BASE COUNT          916 a         1496 c         1471 g         1158 t
ORIGIN      
        1 tccttctggc cggccatcgc cgtcacgatc aactcagcgc tcctcgggtc gatgtagagc
       61 cgggagacga gagaaccata gtcggtggta tcgagccacc ctccaacatc ggtgatcatc
      121 tcggcttcca gcaggaagtc gagcacccga tcgacgaccc tctttaatgc gagtgggttc
      181 tcgccctggt gtgcgtagaa agtcccgtcc atgaaaccca tgacctcgtc acgctcgcgt
      241 gcaaaacctg tcgcgatgag tgagagtatg tgggtgcaga ggatagcctc gttggcgcag
      301 cggctccgga catcctccgg tggcgcctcg atgtagcaat caaagagttc gtcgaccata
      361 tcgtcggact tcgctataag gacagcctcg ccatatgggt cgaggtgtgg ccgcccggca
      421 cgtccggcca tctgccgata ctccctgaca gggatgggga ccatcccctc gccggcgttg
      481 aaccgcatgt agtcgcggac gataaccctt cttgcaggga gattcagccc ggcggcgagg
      541 gttggggtgg aggcgatcac cttgatctgc ccctccctga acccctgctc gacgatctgg
      601 cgctcttccc gtggaagccc agcgtgatgg aatgctgcac ccgctgcaac gcaggcggcc
      661 agggtctttc ccatatcggt cgcggcattc gagcggatcc tgtctgcgta tccggcaagg
      721 atggggtttg aaagtttaag tcccgatgct gcgcgttttg caaacgcctc cgcgttcttg
      781 cgggaactga caaagaccag acactgcccc ccctcagcga ccgtgtcgag gacaaggtcc
      841 aggtcctcgt gtttgctcct gcgctcgatc tggcggatgc tgtcggtgaa gtggatagtg
      901 tcgagaagga agaccccctc ccggagttcg accggccgcc agtcgctctc tacgagttcg
      961 gcgtccagcc atccggcgag atcccccggg tttccgatgg tcgccgagag ggcgatgatc
     1021 tgcaccccct cgtttttatg ccggagtttt gtaataacca tctcaagcgt cgctccccgg
     1081 gaggggtcgt cgatcaggtg aacctcatcg ataaccagga ggctgatctc cgagagccag
     1141 ggtgttcggt tacgcaggag cgagtcgacc ttctcgcttg ttgcaactat gatatcgttt
     1201 cgccccaggt actcgtcccg ccggtcgaag tcccctgtgg cgatcccgac acggagccct
     1261 ttccccgaga actcctcgaa cttctcgctt gccagagccc ggagtggaac tatgtagagg
     1321 catttaccgc catttgccac ctggtggtgc atcgccatct cggctaccag ggtctttccg
     1381 ctcgccgtgg ggatggcgat gaggaggttt ttgcccgaga aaagccctgc ctcgacgcag
     1441 gccgcctgcg gcgggtataa ctctctgatc ccgtggtcgg tgtaactggc tgcaagttgc
     1501 gccgggagag ggaggtcggc tatctccata tacacccctt catttccact gcaaactgta
     1561 taactccctg tatcgttaca accccggatg gagaggggtt cgccgctcca gtatcacgag
     1621 taatactcca gcatctcctg cctggcttcc ttctttttcc ggtcgaggaa agcgtagaca
     1681 gtctcgtggg gcacgccctg gatcagcatt tccactgctt tcctcgcgat ctcaacctgc
     1741 tccgggaggc cgatgatcgc tacggtcttc ccgtagaccg agatctctgt cccggtcata
     1801 tcttcgatct gggcacgaga tgtgccggat ttccctataa tcctgccccg gagccgttcg
     1861 aggtgccggg ttgtccccgc caggtcggtg agatcgatga gaacgaggat catgtcctcc
     1921 tcatcgagga gcctgaatgc acgctcaggc gagaaccccc tgccgatcgc ggatacgacg
     1981 tttgctgccg tcacgacccc gaccgggtca tccccctcga tnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn ngcgtgcctg cgggtgatcc agggttaccg cctgccccat
     2161 atcgatcagg tatggtttct cgtggtagag gatattgtat tcggataggt cggcgtgaac
     2221 caggcgggcc tccctgtata gtagatccac ataagcgagt atctcgttgt agaccgcccc
     2281 gtagtcctca acttcggcgt tccggagttg cgggtatggg atctcgtctt cacccaggaa
     2341 ctccatcagg agtatgtttc ggtcgaacgc cagcggttca ggggttggaa ccccggcctc
     2401 gtgtgccctg gccaggttgc tgaactcctt cttcgtccag gcgaagatta tacccttgcg
     2461 cgtcccacgg acgcttgaga accgccggtc tcctgccagg tagtcggtca tcgccttgaa
     2521 gttcccggtc tggatccggt agatcttgat cgctatcccc tgcgcgccac gttcaccgta
     2581 gaagacgttt gcctctttcc cggtgctgat aggcccgccg atgcttgaga tcagtttcct
     2641 gtggacgagt ttgtagagcg cgataagggt gacctcgtcg aagacgtctt cgcgtacctt
     2701 cagggagtct gcgtctttta ttttgactcc catctccgcg atctcgcggt cgaaccgttc
     2761 atactcttcg ttgcggctca cggccgaatc acctgctgaa gacgtaatcg tgcactgggg
     2821 cgaggatatc tatgagatgg ctggcggtgg ctgccttaag gtccattgga tggatctccc
     2881 cggcggcgta ggcgcgttcg aggtcttcgt aggtgtaaaa ctcccgatca ccgccgaact
     2941 tcgcgggccg gcggatggtg atctttccgc tccgcgggaa gacgtggtag cgcaggatct
     3001 caagcaccgg attgttctcg atctccggcg ggcagaacgc tctcttaatc tttcgctgga
     3061 tctcctcatc agaatcggcg accgatatca cgttcccggc tgaggatgac atcttcttac
     3121 catcaaggcc gtttatgatc ggtgtatgga tacatactgg tgccggatac ccgatcgagg
     3181 ggaggtgctc ccgcgcgagc atatggatct tccgctggtc gatcccgccc acagcggcgt
     3241 ccacctccag cgtcgctata tcgaccatct gcatgatggg gtatatcatc tgggagacca
     3301 tcgggttctc catctgccgc ccgacctcgt ccatgctcct ctttgcgcgg ttgagggtga
     3361 ctctctggga gagttcgagg acggcaagtt cgtagtccga tgttaactgc acctccgagc
     3421 ccaggacgta tcttgcgcct gaaagcccga gcccctcaaa acatcggcgg ttgtactcgg
     3481 ctatctcccg gatctcctcc atcgtcccct tgcggttcag gaacgcatgg aggtcggcga
     3541 taagcacggt cacctcgaac cccgcctgct gcaggtccat cagtttgttc accgtcacca
     3601 tgtggccgag gtgaatctcc ccgctcggct cgtagccggt gtagacccgc ctgacagggc
     3661 ggtcgaggag cgcccgaagt tcctccccgg ttacaacctc aacagtatta cgggtcgcca
     3721 gtttgtatgg atccattaga aaagattggt ctgtcgggga gttaatcctt cttcctcccc
     3781 ctgtcctctc gttcagccta tgggaacgag accgagcgct tcgttggtca tccggatgga
     3841 ctcttccgga tccttcaccg ttccgagcat cgcgcggatg caatcgatgt tttcggggat
     3901 gacatcggcc tcctggtgga tcgcctggaa gcagtagaaa tccttcccgt tcaggacggc
     3961 aaccgactct tcaaagacgc cgttctccca gaggtcggtt ctcggccggc cgatgtcctg
     4021 ggtatactca cggagctggg cgttgcttac gatcccggtt gccttgcgga tgagcccgat
     4081 ccggttgtgc ctctcaaaga ccttcagcac ctcttcgcgc gtggtcttgg atttcagtct
     4141 catctgtact gtgtgcatgt gcataaacgt cgtggggacg atcatcgcca gtgtgactat
     4201 ttcgatgtgc ggtaggacgg tctggacatc gggcccgtga tggcttggga tggtggccgg
     4261 gttgaggaca atggcatcga ccggtcccct tttaatatcg tcggggtccg cagctctccg
     4321 aaccatcacc gccatgaccc gctcgactcc gaaggcctga tccagggcgt ggatgagccg
     4381 tatgagtccg gtggagttgc acgagacaac ccgcgcgaac tgttttcctt cagcctcgcg
     4441 gtaatttgcg tgggcgttga aggagaagcc ggcaacctcg tgctcctcgc cgccctggaa
     4501 gattgctttt ttaccgagac gttcatagat cggccgattc ttctctccta cccctccggg
     4561 tgtggcatcc acgacgatat cggcggccct gatcatggtc tcgacatcgc cggcaacctc
     4621 aagcccggcc ttctcgaatg ccggtcttct gctgatgtct gcgatgtaga ggggataacc
     4681 cctctctttt gcaatgtatg cctcggcgga tacgctggtc ttagataccc cgataacctc
     4741 catatcgggc tgcgcggcga cagcatcggc gacccgtttg ccgatggtgc cgtaaccgtt
     4801 gatagcgact ttgatcatgc aagagggaat acagaacaag aattaataaa ggttgctggt
     4861 ccgcaaggag gaagatgggg aggccatcaa accaacagcc acactggttt gataaacctc
     4921 tccgtggaca gtccacggac gatccatgga cagattccgg ggggtggccg ggatctgtat
     4981 gaagatgctg cccccatctt catgcggtat gcctctggtc acagcggcat catgcggttt
     5041 tttgcgagac ggcattgagg ggaggtggga gagggaggcg ggggttggtc gactgctcgc
     5101 atggaaagac gtcttcggtg agacacagga gacaggggcg g
//
LOCUS       DAOSZX010000101         5067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13766_length_5067_cov_22.577015, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000101 DAOSZX010000000
VERSION     DAOSZX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13766_length_5067_cov_22.577015"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PK196_07270"
     CDS             complement(<1..1121)
                     /locus_tag="PK196_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41252.1"
                     /translation="MLQMISWLDGNFRSLQPTRATIMRALRHLRPADRKNLFSSDIPQ
                     MRTAEGRWFEAIVYEMFLELSLQTDLIQAVVARGADGPTKVRRAQLGQNGLFYSNIGD
                     IKVRGNGQDLAEVDLVLVDHTGSLTFGEIVTSPADLKELEEEIHYKKHLFGYLYGQPI
                     VPFLLISSVDISKTAVVRRLMKEPDNVILTTPTCEELKTLIRQSDLKRSPVRRVRHDK
                     LISISDIPPRRPFDYKKLHDERLQSIIATVTSKSGARELGRPDEIPPIVKKVIFGGLY
                     PSAVRMLDAQYPIQIRGETYDPDMIQKRFSKVVLAVNIPEYRPIIYLRTRNKREYLKM
                     VPSKTGGFRFESRRTPHMAGFFLWLESIQPSVGAELTRDL"
     gene            1590..3212
                     /locus_tag="PK196_07275"
     CDS             1590..3212
                     /locus_tag="PK196_07275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HON41253.1"
                     /translation="MGQNKIYIIGHKQPDTDSICSVIGYAELCNHSEPGKYIPARCGE
                     VGPEARFALEKFNVEPPIYIASVEPTVSDIPLDTRCARQDLPAIDVAAMMDTYDMRNM
                     PITDDRGRLVGLVSEYGLARAYVRKNPREQLSLVPMSIETLARILDARVVVQAREMLG
                     EGRVYTVIDALHVTLSRMTPDDIAIVGDNEPAQLALISAGVAALIIAEGAPVGERVIE
                     AARARGASVFATTLDAFSVGKMISLSLPARMIMETDVPTVRLEDSLDYAKDVVSNSKF
                     RTACVVGEDGEHIGLVSRTTLMQDVQKSVILLDHNEYSQAVDGIEKADILEIIDHHRL
                     GAISTLKPVKFLNDPVGSISTIIANKFIEAGLEPTPSTAGVLLAGILSDTMVMRLSTT
                     TPADIRAADYLAEIAGVDPAVFGAELIKKGMDLDSLPKDELLTRDMKRYNLFGKSVMV
                     SQVMTSSFDFSQNHADEIRAELERLRTAFGVDCFFALFTNIFENASDLFAAGDDGCVS
                     RLGLRDQPLRLEGVMSRKNDFIPKFGRMLRQV"
     gene            complement(3228..4553)
                     /locus_tag="PK196_07280"
     CDS             complement(3228..4553)
                     /locus_tag="PK196_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON41254.1"
                     /translation="MIIVIGGGPAGRLGAMHLAQAGREVRLVEQRKIGGQCLHDRCMT
                     ICALNDVARLIEQTRTQKDLGILDCVPTISYPMIWRRMREVQERLSSVLDAETREAGV
                     EIVYGAESRLEGGRVFIDDEEVRAEAVLAATGSRAAIPDIPGTDLPGVYTYQTLPAMS
                     DLPRRMAVIGGGVVAAEFAYIFNAFGVEVELIARHGLLKDLDPRMRSAALRDLAGVGI
                     REQTTVASIEGGGRVRSVILANGEEVEAGAVLLATGLVPRSESLQGLLKRPDGAVIVD
                     QRMQTSVENVYAAGDVIGPPYLTPAARREGLVAAENILGHEAVMDYRCIPQAISLRYD
                     LAFASTDGDDGALTLSAPAPSGSGSFWDVATGWTGLARIQVDPESGRLVGAAAAVPGA
                     SSLFSYLAYLMKQGITVADFADIIEVHPSTDGIYWLARYAGDILQQKKR"
     gene            complement(4562..>5067)
                     /locus_tag="PK196_07285"
     CDS             complement(4562..>5067)
                     /locus_tag="PK196_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41255.1"
                     /translation="EKRACKIAGIQGTAKCRFIPYWSYNYVSRGEREVKNRAISFDAE
                     GRGAINAINGLKMEIDPGEIKRGPIPAGSEIVPPRLMREDVEEEVVQEVTELLTQRVR
                     FRYEKGDTIFYEEKTLKPDRNNIRVELETVYVPVWQVRGGSKIIEVNAFSGEILSMPM
                     DEGVELL"
BASE COUNT         1004 a         1549 c         1452 g         1062 t
ORIGIN      
        1 aggtcccgcg tcagttcggc cccgaccgag ggctggattg actcgagcca gaggaagaat
       61 cccgccatgt ggggcgtccg ccgactctcg aatctaaatc cgccggtctt gctcggcacc
      121 atcttgaggt actccctctt attccttgtc cgcaggtaga tgatcggccg gtactccggg
      181 atgttcaccg cgaggacaac ctttgagaac cttttctgga tcatatcagg gtcgtaggtc
      241 tctcccctga tctggatggg gtattgggcg tcgagcatcc ggacggcgga ggggtagagc
      301 cccccgaaga tgaccttctt cacgatcggc gggatctcgt cgggtctgcc gagttcccgc
      361 gcaccgctct ttgatgtgac cgtcgctatg atgctctgca gccgctcgtc atgcagtttt
      421 ttgtaatcaa acggacggcg gggaggaata tcactgatgg agatcaactt atcgtgccgg
      481 actcgccgta ccgggctgcg tttcaggtca ctctgcctga tcagggtctt taactcctcg
      541 caggtcggcg tcgttaggat gacgttgtcc ggctctttca tgaggcgacg gacgacagcc
      601 gtcttggaga tatcgacaga ggatatgaga agaaacggca cgatcggctg cccgtagagg
      661 tatccgaaga ggtgcttttt gtagtggatc tcctcctcaa gttcttttaa gtcggcaggt
      721 gaggtgacta tctccccgaa tgtcaacgat cccgtatggt ccacgagcac gaggtccacc
      781 tcggcgagat cctggccgtt gccgcggacc ttgatgtccc cgatgttcga gtagaacagt
      841 ccgttctggc cgagttgcgc gcgcctgact tttgtgggtc catcagcccc ccgcgccaca
      901 acggcctgga tcaggtcagt ctgcaacgac agctccagaa acatctcata gacgatcgcc
      961 tcaaaccagc ggccttcggc agtccgcatc tggggtatgt cgctggagaa gaggttcttt
     1021 cgatctgcag ggcgcaggtg gcgcagcgct ctcatgatgg tcgcacgcgt tggttgaagc
     1081 gacctgaaat ttccgtcaag ccacgaaatc atctgtaaca tctgttccac acagcctggg
     1141 tattgttatc gtattgaggg ccgaatgagg gttggagcac attccatttc tggcgtcgat
     1201 caccatggac gtgctcatat gagtttccgg tttcctgcac tatgaaatta cgcatgaccc
     1261 ccgttgcaga tgggaggctc tcaaaccagt ggcatcaggc tttaccctgg tttgattgac
     1321 ttcttcgggg atggatctgc cggcggctgc gcaatcgccg cacaccgcct gcccgcatct
     1381 gtggtagggg gttgacagaa gagactttga cgggagacgg ttttatgccc cccgcgcata
     1441 tgctgccggc agaaggcgga gagattgtat cctggtcgtt gacctgagga tctcagggtg
     1501 tgccccccct ggacaaaaga tttgatagca gaggttgata cctctaagag ataatcgttc
     1561 tccggccggc ccggggatcc gaggggagta tgggacagaa taagatctac atcatcggtc
     1621 acaaacagcc cgacaccgac agcatctgca gcgtcatcgg ttacgccgaa ctttgcaacc
     1681 actctgaacc cgggaagtac atccccgccc gctgcgggga ggtggggccg gaagcgcggt
     1741 tcgctctcga gaagttcaac gttgagcccc ccatatacat agcgagcgtc gagcccaccg
     1801 tctcggacat cccgctcgat acccggtgcg cccgccagga tctcccggcg atcgatgtgg
     1861 ctgccatgat ggatacttac gatatgcgca acatgccgat cacggacgat agaggaagac
     1921 tggtcggtct ggtcagcgag tatggtcttg cacgggccta cgtccggaag aacccgcggg
     1981 aacaactctc gctggtcccg atgtctattg agactctcgc ccgtatcctg gatgcgcgtg
     2041 tggtcgtcca ggcgcgcgag atgctcgggg aaggccgggt ctacaccgtc atcgacgccc
     2101 tgcacgtcac cctctcgcgg atgactcccg acgacatcgc gatcgtcggg gacaacgagc
     2161 ccgcccagct tgctctgata tcggccgggg ttgccgcgct cataatcgcc gaaggggcgc
     2221 cggtgggtga acgggttatt gaggccgcca gggcgcgggg ggcatcagtc ttcgcgacaa
     2281 cgcttgacgc tttcagcgtc ggcaagatga tcagcctctc gcttcccgcg cgcatgatca
     2341 tggagaccga cgtccccacg gtgcggcttg aagactcact tgattacgca aaagatgtcg
     2401 tctcaaactc caagttcagg acggcctgtg tcgtcgggga agatggagag cacataggac
     2461 tcgtgtcgcg gacgacgctg atgcaggatg tccagaagtc ggtgatcctg ctcgaccaca
     2521 acgagtactc ccaggccgta gacgggatcg agaaggcgga catccttgag atcatagacc
     2581 accaccgtct cggtgcgatc tccaccttaa aaccagtgaa gttcctcaac gatccggtgg
     2641 ggtcgatctc gacgatcatc gcgaacaagt ttatcgaggc tggattggag cccacaccgt
     2701 caaccgccgg ggtcctgctt gctggaatcc tctcggacac aatggtgatg aggctctcaa
     2761 ccacaacccc tgcggatatc agagcagctg actaccttgc ggagatcgca ggggttgatc
     2821 ctgccgtttt cggcgctgaa cttatcaaga agggtatgga tctcgactca ctcccgaaag
     2881 atgaactcct cacgcgggac atgaagcgct acaacctctt cgggaagagc gtcatggtct
     2941 cgcaggtgat gacatcatcg ttcgatttct cacagaacca tgcagatgag atccgtgctg
     3001 aacttgagcg cctgagaacg gcgtttggag tcgactgctt cttcgccctt tttacaaaca
     3061 tctttgagaa cgcaagcgat ctctttgccg ccggcgatga tggttgtgtc tccagactgg
     3121 gactgcgcga ccaacccctc aggctcgagg gtgtcatgtc ccggaaaaac gatttcatcc
     3181 cgaaattcgg gcggatgctc cggcaggtct agaagacccc ctcaaactca cctcttcttc
     3241 tgctgcagta tatccccggc gtatcgggca agccagtaga tcccgtcggt cgagggatgc
     3301 acctcgatga tatcggcaaa atcggccacc gtgatgccct gcttcatcag ataggctaga
     3361 tacgagaaga gggacgatgc cccgggaacg gcggctgccg ccccgacgag cctgccgctc
     3421 tcaggatcaa cctggatacg tgcaagtcct gtccacccgg ttgccacatc ccagaatgag
     3481 cccgacccgg acggcgccgg agcagatagg gtgagcgctc catcatctcc gtcggtcgag
     3541 gcgaaagcga ggtcgtagcg gagagatatt gcctgcggga tgcatctgta atccattaca
     3601 gcctcgtgac caaggatgtt ctcggccgca accagcccct cccgccgggc ggcgggtgtg
     3661 aggtatggcg gacctatgac gtcacctgcg gcatatacat tctcaacgct cgtttgcatc
     3721 ctctgatcca caatgaccgc gccgtccggc cgcttcaaaa gaccctggag cgactccgaa
     3781 cggggtacaa gacccgtcgc cagcaggact gccccggcct cgacctcttc gccgttagca
     3841 aggatgaccg agcggacgcg tccgcccccc tcgatgctcg cgacagtggt ctgctcccga
     3901 atcccgaccc cggcaaggtc gcgcagcgct gcagaccgca tcctgggatc tagatccttg
     3961 agcagtccat gccgggcgat caactcgacc tcaactccga aggcattgaa tatgtaggca
     4021 aactcagccg cgaccacccc gccgcctatc accgccatgc ggcgggggag gtctgacatt
     4081 gcgggaagcg tctgataggt gtataccccc gggagatcgg tgcccggaat gtcggggatc
     4141 gccgcccgcg accccgtcgc cgccaggacg gcctccgccc tgacctcttc atcgtcgatg
     4201 aagacccggc ccccctcaag ccgactttca gccccatata cgatctcgac ccccgcctcc
     4261 cgcgtctcgg cgtcgaggac ggaggagagt ctctcctgaa cctcgcgcat ccgtctccag
     4321 atcatggggt aggagatggt cggaacgcag tcaaggatcc cgagatcctt ctgtgtcctg
     4381 gtctgctcta tcagccgtgc cacgtcgttt aaagcgcaga tcgtcatgca ccggtcgtgg
     4441 agacactgcc ctccgatctt gcgctgctcg acaagccgga cctccctgcc agcctgcgca
     4501 aggtgcatcg caccgagcct gcctgcgggc cctccaccga tgacgatgat catgggttta
     4561 cctacagaag ttcaactccc tcgtccatcg gcatagagag gatctcgccg ctgaacgcgt
     4621 tgacctcgat aatcttgctc ccgcccctga cctgccagac gggcacgtag acggtctcaa
     4681 gttcgaccct gatattgtta cggtccggtt tgagggtctt ctcctcgtaa aatattgtat
     4741 cccccttctc gtagcggaac cggacccgct gggttaagag ttcggtgacc tcctgcacga
     4801 cctcctcctc cacatcctca cgcatgaggc ggggaggcac gatctcggag ccagctggga
     4861 ttggaccacg cttgatctcg ccagggtcga tctccatctt caggccgttg atggcgttga
     4921 tcgcgcccct cccctcagca tcaaaggaga tcgcccggtt cttgacctcg cgctcgcccc
     4981 tgctcacgta gttgtaactc cagtatggga tgaaccggca tttcgctgtc ccctgtatcc
     5041 ctgcgatttt acaggcgcgc ttctccg
//
LOCUS       DAOSZX010000102         5041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_13852_length_5041_cov_21.895708, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000102 DAOSZX010000000
VERSION     DAOSZX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_5041_cov_21.895708"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /gene="nrdD"
                     /locus_tag="PK196_07290"
     CDS             complement(<1..555)
                     /gene="nrdD"
                     /locus_tag="PK196_07290"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HON41256.1"
                     /translation="MRLSRKSTQTTLFGEFVPTFPKVRTSRGYLLDWDRNRIVRQIVE
                     ETRLVEVFYGYKGADEETAKEIARLVEKKIQMLGLQSLSGPLIREIVNMTLLERGLIP
                     YRNVCTRVGTPVFDAHLIDVGRGFEAHDNANLQENAETSHKKKADKISKEQYLLQLPP
                     DLADHHLQGDLHIHDLEYFGTRPFC"
     gene            complement(552..821)
                     /locus_tag="PK196_07295"
     CDS             complement(552..821)
                     /locus_tag="PK196_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HON41257.1"
                     /translation="MSNTPQFIVYTLESCPNCEILKEFLASRGVPFIECDMASAEALT
                     ELRMNGVFVQEAPVLQKGEMFYTSADLFPRGVFREDLVAGLIAEA"
     gene            1027..1569
                     /gene="thpR"
                     /locus_tag="PK196_07300"
     CDS             1027..1569
                     /gene="thpR"
                     /locus_tag="PK196_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HON41258.1"
                     /translation="MVRTFVAIDLTEEIRQRARESQEILREAPGRLAIVEPENLHLTL
                     KFLGEVDQAQIEPIIGALRAVRADPFNLTVGYAVCNHPQRPRVIWCDVTDSGKSAALA
                     RQVDDLLEPLGFPREKRPFRPHVTLARVKDLHPALTKEVERRIPREPLGRCLVSAVKL
                     KKSTLTPGGPIYEDLAEVTL"
     gene            1593..2942
                     /gene="cca"
                     /locus_tag="PK196_07305"
     CDS             1593..2942
                     /gene="cca"
                     /locus_tag="PK196_07305"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HON41259.1"
                     /translation="MLRRIRPTPEERTYIRAMGERLIEAVERSGKAKAMMVGSVARDT
                     FVRGDRDLDIFILFDPSLSRQELQEEGLSLARRIAEEFGATWREKYAEHPYLNATIDT
                     LDIDLVPCYAVASATEIKSAVDRTPFHTRYVLAHIDGYVDDVLLLKQFAKASGVYGSD
                     HMTGGFSGYLCEILTIYYGGFHPLLEAAASWKPGEVIDIEGHGTKEFDDPLVVIDPVD
                     PGRNVAAALSLSRMFEFVEVARGYLEEPSEAFFCRPPSPALTPVVFSRLLSARGTYLY
                     SITFKTPDLTPDTVVPQLRKSAESIRDLLERSGFPVNRTDVCMREKRCMLLFELMAET
                     VPVMRRHIGPPLWSRENSRKFLEKYVRKEVFAGPYIEEGRYVVEVARRFTRAIDLLRS
                     NVILDIALGKHVRRSMEEGWSVGTGAECWDEEFAVFLSRFLDRASPLARIKRMAARR"
     gene            complement(3051..3383)
                     /locus_tag="PK196_07310"
     CDS             complement(3051..3383)
                     /locus_tag="PK196_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41260.1"
                     /translation="MNSAVDSFESRTQIPGPGGMLLGTVYVDISDEEWAVAIAYGRAH
                     HPGIRGPEPVYEVRYAYRPEGEGAIKRLDTRKGTPSTVTVGPLHSVDEFVVLALDYEK
                     KRIGGTGP"
     gene            3711..5039
                     /gene="glnA"
                     /locus_tag="PK196_07315"
     CDS             3711..5039
                     /gene="glnA"
                     /locus_tag="PK196_07315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HON41261.1"
                     /translation="MSRDAISTMLDRIEQDKVRFLRLQFTDLLGMPKNVAIPVKQAEK
                     ALTGGIGFDGSSIEGFVRIEESDMILKPDLSTYTLLPWQPADYAEARFICDVYKANGR
                     PFEGDPRYVLRRAMEDAAKDGFVFNTGPELEFFLFKMIDGRPTTLFQDVGGYFDLAPT
                     DLAEDVRREIILALTEMGFEIEASHHEVAESQHEIDFKYSDALHTADNVITFKFAAKT
                     IALMRGLHASFMAKPIYGICGSGMHTNCSLAKDGKNAFFDPDAPIQISETCLHFIGGL
                     LKHARAITRLANPTINSYKRLVPGYEAPCYVSWSASNRSALVRVPAPRGNSTRVEFRS
                     PDPTCNPYLAFAAMLTAGMDGVRNKIEPPNGVDKNIYQMTPAERGHAGIDTLPCDLYE
                     AHRALLADNLICEALGPHVLEALTSVTNAEWDAYRTAVHPWELDRYLATY"
BASE COUNT         1031 a         1527 c         1468 g         1015 t
ORIGIN      
        1 acaaaacggc cgggtaccaa aatactccag gtcgtggatg tggagatccc cctggaggtg
       61 gtggtctgca aggtcggggg ggagctgcag gaggtactgc tccttgctta tcttgtcagc
      121 cttcttcttg tgtgaagtct cggcgttctc ctggagattg gcgttgtcgt gcgcctcaaa
      181 tccccgccct acatcgatga ggtgggcatc gaagaccggg gttcccaccc tggtgcagac
      241 gttacggtac gggatcaggc cgcgctcaag gagcgtcatg ttgacgatct cgcggatcag
      301 gggccctgag agggactgga gtccaagcat ctggatcttc ttctcgacca gtcgggcaat
      361 ctcctttgct gtctcctcat cggccccctt gtagccgtag aagacctcaa caagccgggt
      421 ctcctccacg atctgtctga cgatccggtt cctgtcccag tcaaggaggt agccccgcga
      481 cgtcctgacc ttcgggaatg tagggacaaa ctccccaaac agcgtcgtct gtgttgactt
      541 acggctcaac ctcatgcctc cgcgatcaga ccggcaacca ggtcctcccg gaagacgccc
      601 cggggaaaga ggtcagctga tgtatagaac atctcaccct tctgcagcac cggcgcctcc
      661 tgaacgaata ccccgttcat gcgaagctcg gtcagcgctt cagcggatgc catgtcgcac
      721 tcgatgaagg gcacgcctct cgaggccaga aattctttca ggatctcgca attcgggcag
      781 gattccagtg tatatacgat gaattgcggt gtattggata tggtaatacc cccggatcat
      841 tcggtcatta tggtttcgtt ctggagcgtt ataacaggta tcattctttt ttgagcagtg
      901 cgggatggac ggcaaaaccc ggtatcgtga acgcccgtgg acttgcagcc tcccatcctc
      961 cttcaagccg gttttccggg tagacaatca ccttttgtat ggtgaggcaa gataatacgg
     1021 tacagtatgg tcaggacgtt cgttgcaatc gatctgacgg aggagatccg ccagcgcgcc
     1081 cgcgagtcgc aggagatcct gagagaggcg cccggccgac tcgccatagt cgagcccgaa
     1141 aatctccatc tcacgctcaa gttccttgga gaggtcgatc aggcgcagat cgagccgatc
     1201 atcggggcgc tccgggctgt cagggccgac cccttcaacc tgacggtcgg ttacgcggtc
     1261 tgcaaccacc cgcagagacc gcgggtgatc tggtgcgatg tcaccgactc ggggaagagc
     1321 gctgcgctgg cccggcaggt ggacgacctc ctggagccgc tcgggttccc cagggagaaa
     1381 cgtccattcc gcccccatgt aacacttgca cgcgtaaaag atctccaccc cgcgctcaca
     1441 aaggaggtgg agcgccgtat accccgcgaa ccgctcggga ggtgtctggt gagcgccgta
     1501 aaactcaaga aaagcacgct gacacccggc ggtccgatct acgaggacct tgcggaggtc
     1561 acgctttgac gcggttcccc tgtgaggagg aggtgctcag gaggatccgt ccaacacctg
     1621 aggagcggac ctacatccgg gcgatggggg agcgcctgat cgaggcggtt gaacgatcgg
     1681 ggaaggcgaa agcgatgatg gtgggttcgg tcgccaggga taccttcgtc cggggtgacc
     1741 gtgacctgga catcttcatt ctctttgacc cctccctctc ccgccaggaa ctgcaggagg
     1801 aggggctatc gctcgcacgc cggatcgcgg aagagtttgg cgcaacgtgg cgggagaagt
     1861 acgcagaaca cccgtacctc aacgcaacga tagacactct ggatatcgat ctcgtcccct
     1921 gctacgcggt agcgagcgcg accgagatca agagcgccgt tgaccgaacg ccgttccata
     1981 cacgctatgt ccttgctcat atcgatggct acgtcgacga cgtcctccta ttaaagcagt
     2041 ttgcaaaggc cagcggggtc tacggttcag accatatgac cggcgggttc tccggttacc
     2101 tctgcgagat cctgactatc tactacggcg ggttccaccc tctcctcgag gccgcagcca
     2161 gctggaaacc gggggaggtg atagatatcg aggggcacgg gacgaaggag ttcgacgacc
     2221 ccctcgtggt gatcgatccg gtcgacccgg ggagaaacgt cgcggcggcg ctatcgcttt
     2281 cgcggatgtt tgagttcgtg gaggttgcac gcggctacct cgaggagccc tcggaggcgt
     2341 tcttctgccg cccgccctca ccggcgctaa ccccggtggt cttctcccgc ctcctctctg
     2401 ccagggggac atacctgtat tctatcacct tcaaaacacc cgatctcacc ccagatacgg
     2461 tggtgcctca actacggaaa tctgccgagt ccatacgcga cctcctggag aggagcgggt
     2521 ttccggtgaa ccgtaccgac gtctgcatga gggagaagcg gtgcatgctg ctctttgaac
     2581 tgatggcaga aaccgtgccg gttatgcgca ggcatatcgg cccgccgctc tggtcgaggg
     2641 agaactcccg gaagttcctg gagaagtatg tcaggaaaga ggtctttgcc ggaccataca
     2701 tcgaggaggg gcgatacgtg gttgaggtgg cccgcaggtt tacccgggcc atcgatctgc
     2761 tgcggtcaaa cgtcatcctt gacatcgcgc ttggaaagca cgtccggcgc tctatggaag
     2821 agggatggag cgtggggacc ggcgctgagt gctgggatga ggagtttgcc gtcttcctct
     2881 cacggttcct ggaccgcgca tcgcctcttg ccaggataaa gaggatggca gcacggaggt
     2941 gagagactcc cctccctgcc atcccgaacc ggcgggttct ccagaggggc acaccccgaa
     3001 gacgccaaga tcctcgatgg tgcgcggcct atgccgcgca ggagattgct ttacgggccc
     3061 gttccgccga tccgcttctt ctcgtagtca agagccagga ccacaaactc gtccactgag
     3121 tggagggggc cgaccgtaac ggtggacgga gtacccttcc gcgtgtcaag cctctttatc
     3181 gcgccttccc cttccggccg gtaggcgtac ctgacctcat agacgggttc aggaccccga
     3241 atgccggggt ggtgcgctct gccataagcg attgcaacag cccactcttc gtcgcttatg
     3301 tccacgtaga ccgtccctaa cagcatcccg ccggggccag gaatctgcgt tctcgattca
     3361 aatgagtcca cggcactatt cacggtgcct ttcggagagt atccataata acgtgccatt
     3421 cttaacctta cctccggttg tgtctttgcc ggtgacgtta cagatcccac ggccgcccat
     3481 cacccccatg ccggagcatt atcctgcgga gccacaggag gggagcggtg gccggtgtgt
     3541 aacacaagta tattggtatt agatctattt aatttgatca cattgaaacc gctacgctac
     3601 agcaggaggc tatctaacaa tatcatctga ctgctgtaac ctggtctggt tcttcgtggt
     3661 cctgtggggt caacatgtat ttactccatc gggtagatgt tgatttccac atgtcccgtg
     3721 atgccatctc cacgatgctc gatcgcatag aacaggataa agtccgattc ctccgccttc
     3781 agtttaccga cctcctgggc atgcccaaga acgtggctat cccggtaaaa caggcggaga
     3841 aggcgctgac aggcggtata gggtttgacg ggtcgtcgat cgaggggttc gtcaggatag
     3901 aggagtccga catgatcctc aagccagacc tctcaaccta caccctgctg ccctggcagc
     3961 cagctgatta cgcggaggcg cgtttcatct gcgatgtcta caaggccaac ggcagaccgt
     4021 ttgagggcga cccgcgctac gtgctccgcc gggcaatgga ggacgcggcg aaggacggtt
     4081 tcgtcttcaa cacaggcccg gaactggagt tcttcctctt caagatgatc gatggcagac
     4141 cgaccacgct gttccaggat gtcggcggct acttcgacct cgcgccgacc gaccttgccg
     4201 aggatgtcag gcgcgagata atcctcgccc tgaccgagat ggggtttgag atcgaggcct
     4261 cgcaccacga ggtcgccgag agccagcacg agatcgactt caagtacagc gacgccctcc
     4321 acacagcaga caacgtcatc acattcaagt tcgccgcaaa gacgattgca cttatgcgcg
     4381 ggctgcacgc ctccttcatg gcaaagccca tatacgggat ctgcgggagc gggatgcaca
     4441 ccaactgctc gcttgcaaaa gacggcaaaa acgcgttctt tgacccggac gctccaatcc
     4501 agatctcaga gacctgtctc cacttcatcg gcgggctgct aaaacatgcc cgtgccatca
     4561 cccggctcgc gaaccccacg atcaactcct acaagaggct cgtcccgggc tacgaggcgc
     4621 catgctacgt cagctggagc gcaagcaacc gctcggccct ggtccgcgtc ccggcgccgc
     4681 gcggcaacag cacccgggtc gagttccgca gccccgaccc tacctgcaac ccctacctcg
     4741 ccttcgccgc gatgctgacc gccggtatgg atggtgtgcg caacaagatc gaacccccga
     4801 acggtgttga taagaacatc taccagatga cacccgctga gcggggtcat gccgggatcg
     4861 atactctgcc atgcgatctc tatgaagcgc accgggcgct gctcgccgac aacctgatct
     4921 gtgaggctct tggccctcac gttctggagg ccttaacgag cgtcaccaac gccgagtggg
     4981 acgcatacag gactgcagtc cacccctggg aacttgaccg gtacctggcg acatactgaa
     5041 a
//
LOCUS       DAOSZX010000103         4863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_14470_length_4863_cov_20.936980, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000103 DAOSZX010000000
VERSION     DAOSZX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14470_length_4863_cov_20.936980"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1244)
                     /locus_tag="PK196_07320"
     CDS             complement(165..1244)
                     /locus_tag="PK196_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HON41262.1"
                     /translation="MPERIIVPFGPQHPVLPEPIHIDLVLEDEKVVEAIPSIGYIHRG
                     LEQLVQKREYPEFVYVAERICGICSFIHSLCYCQGIEKIMEIEVPDRARYLRTIWSEY
                     SRLHSHLLWLGLFADSMGFENLFMRSWQLREKVLDVLEDTTGGRVIQGTCKVGGVRRD
                     IVQEKLDEMHRGLESFEEECRDLGHVFLNDNTVNYRLRGLGVLSKAEAYDLGAVGPTA
                     RASGVEMDHRETGYAAYGDLGFKPVIETAGDCYARCAVRVGEVYASIDLIRRAIEEMP
                     EGPIDVKVRGTPDGEYFSRAEQPRGEVVHYLRGNGTKHLVRDRIRTPTLTNIPSLVKM
                     LPGVQLADVPVVVLSIDPCISCTER"
     gene            complement(1229..1609)
                     /locus_tag="PK196_07325"
     CDS             complement(1229..1609)
                     /locus_tag="PK196_07325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HON41263.1"
                     /translation="MTIQEEQTIIEVALPDLLREVERLHADGYRLVQIGCTGIGGAYE
                     INYSFDKGYHLRNLRLNVGPDTEVPSISGIYWGAFVYENEMHDLFGIPVSGINIDFKG
                     TFIRTAKKYPFSVARKGEDACQNE"
     gene            complement(1606..2070)
                     /locus_tag="PK196_07330"
     CDS             complement(1606..2070)
                     /locus_tag="PK196_07330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HON41264.1"
                     /translation="MAFLKRSPWILHYDASSCNGCDIEVLACLTPLYDVERFGIINTG
                     NPKHADILLITGGVNEQNRQVVKNLYEQMPEPRVVVAVGICAASGGIFRECYNIVGGV
                     DRVIPVDVYVPGCAARPEQIIDGVVRALSILEEKRAKMKETAQHSEEAGETA"
     gene            complement(2076..2942)
                     /locus_tag="PK196_07335"
     CDS             complement(2076..2942)
                     /locus_tag="PK196_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HON41265.1"
                     /translation="MTWAWLIGAVLFLILAPLAGGLIAGIDRKITARMQGRVGPPLLQ
                     PFYDVGKLFEKERVVVTTAQNFYVLAYLVFIALAGALFFAGGDLLLTIFAFTLAHVFL
                     VLGAYAAHSPYSHIGAERELIQVMAYEPMVILAAVGLYMVANSFYVADIAAVAVPAIL
                     YLPGVFLGLAIILTIKLRKSPFDISTSHHAHQEIVKGITTEFSGPTLGTIEIAHWYEN
                     IFLLGLVYLFFAWNPVVGVIAVVLTYLVEIFVDNTTARVRWQAALKTGWFAALIGIVN
                     LAILSYMMIGGV"
     gene            complement(2939..4843)
                     /locus_tag="PK196_07340"
     CDS             complement(2939..4843)
                     /locus_tag="PK196_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HON41266.1"
                     /translation="MQTLLFLILFPIIAACLLLFVKRTALRYVVVVISALAIGGATIY
                     LLLRHISAGAVYFAVPSGTVDPLMVVIEMGLALFIIYMGVKFKNYLAIALAAVQAALV
                     VYLETTYSGSLHVVNNLFVDQFSIIMALIIGIIGSLIAVYSVSYMETYHHHHPEVRDR
                     QRTFFFVIFIFLAAMFGLVFSNNLMWVFFFWEITTITSFLLIGYAETEEATKNAFLAL
                     VMNLLGGIAFVAALIILAATEPLSGGIDLAGVLSSGDAAVILLPAALIGFAGITKAAL
                     MPFSSWLLGAMVAPTPVSALLHSSTMVKAGVYILVRFAPVFAGTLTGFSIGLVGGLTF
                     VLASAIAISQSNAKRVLAYSTIANLGLIAACAGVGTYMLVWAAILLIIFHAIAKALLF
                     LGTGMIEHRIGSRDIEDMEGLIVRMPRMAVMMFIGMAGMFLAPFGMLISKWAAIEAFV
                     QAPFGLIFIAILAYGSAVTVFFWAKWMGKLVAVTREQERVEKGILEEPWAPLYILTGL
                     VVAATLLFPLISSVLIEPYVLAIYGATARMAQANVAIMLLMMALLLLLPISFLLFKRS
                     AKHLPAYMGGRPATEDRRFAGSLGLTREAQTRNYYLNDYFGEAKLFRPGLAVCTALII
                     ISWILAGVAL"
BASE COUNT         1088 a         1459 c         1406 g          910 t
ORIGIN      
        1 ggagcagagt ccacaggaac ggcacgtggc cgggttgaag acgacgtgcc cccgggtgat
       61 aggactcttc cttgccggta cggcggggta acgccgggtg gccgggcctc ctgcaagatt
      121 tcggagaacc gtccgggtca tctgaaatag actcatcctt gcactcacct ctccgtgcag
      181 ctgatacagg gatcgatcga gaggacaacc accgggacat ccgcaagctg gacgcccggg
      241 agcatcttta cgagcgatgg tatgtttgtc agcgtcggtg tcctgatacg gtcgcgaacc
      301 aggtgttttg tcccgttgcc ccgaaggtag tgaacgacct cgccgcgggg ctgctcagca
      361 cgggagaaat actctccatc cggcgttccc ctgaccttca catcgatcgg gccctccggc
      421 atctcctcga tcgcgcgcct gatcaggtcg attgaggcat agacctctcc gacccttacg
      481 gcacaccggg cgtagcagtc gcctgcggtc tcgatgaccg gtttgaaacc gagatcaccg
      541 tatgcagcgt agccggtctc ccgatgatcc atctcgacac cgcttgccct cgcggtcggg
      601 ccgacagcgc caaggtcata ggcctccgcc tttgacagga caccaagccc gcgaaggcgg
      661 tagttcactg tattgtcgtt taagaagaca tgaccgaggt cccggcactc ctcctcgaaa
      721 gactcaagtc cgcggtgcat ctcgtcaagt ttctcctgca ctatatcccg ccggacaccg
      781 ccgaccttac aggtgccctg gatgacccgg ccgccggtcg tgtcctcaag cacgtctagc
      841 accttctccc ggagctgcca tgagcgcatg aagagattct caaagcccat cgagtcggca
      901 aagagcccga gccagaggag gtgagagtga agacgggagt actctgacca gatcgtccgg
      961 aggtatcgcg cccggtcggg aacctcgatc tccatgatct tctcgatgcc ctggcagtaa
     1021 cagaggctgt ggatgaaact gcagatcccg cagatccttt ctgccacata gacgaactcc
     1081 ggatactcgc gcttctgcac gagctgctca agtccacgat ggatgtagcc gatggacggg
     1141 atcgcctcca ccaccttctc gtcctcaagg acgagatcga tgtggatcgg ctcaggcagc
     1201 acagggtgct gcggcccgaa cggcacgatt attcgttctg gcatgcgtcc tcccccttcc
     1261 ttgcgacgct gaacggatac ttcttagccg tcctgatgaa agtcccctta aagtcgatgt
     1321 tgatcccgga aacagggatc ccaaagaggt cgtgcatctc gttctcgtag acgaacgccc
     1381 cccagtagat cccggagatg cttggaacct ccgtgtctgg cccgacattc agccggaggt
     1441 ttctgaggtg gtagccctta tcaaacgagt agttgatctc atacgccccg ccgatgccgg
     1501 tgcacccgat ctggacaagg cggtatccat cggcatgaag cctctcgacc tcccggagta
     1561 gatccggaag cgcgacctcg atgatcgtct gctcctcctg tatcgtcatg cggtctcacc
     1621 cgcctcttct gaatgctgcg ctgtctcttt catcttcgct cgcttctcct ccaggatcga
     1681 gagagccctc acgacaccat cgatgatctg ctccggcctt gccgcgcagc cggggacgta
     1741 gacgtccacc gggatcactc tgtcgactcc gccaacgatg ttgtagcact cccggaatat
     1801 gccgcccgat gcagcgcata taccgaccgc aacgacaact ctgggctccg gcatctgctc
     1861 atagaggttc ttcacaacct gccggttctg ctcgttgacc ccgccggtga tcagcaggat
     1921 gtccgcgtgc ttggggttcc cggtgttgat gatgccgaac cgctccacat cgtagagcgg
     1981 ggtgaggcac gccagcacct cgatatcgca gccgttacag ctcgacgcat cgtagtgaag
     2041 gatccatggt gatcgcttca ggaatgccat gctactcata cacctccaat catcatatag
     2101 gagaggatcg ccaggttcac gatcccgatc agcgccgcga accacccggt cttaagcgct
     2161 gcctgccacc gcacgcgtgc cgtggtgtta tccacaaaga tctcaacaag atacgtgagc
     2221 acgaccgcga tcaccccgac caccgggttc caggcgaaga agaggtagac caggccgagg
     2281 aggaatatgt tctcgtacca gtgtgcgatc tcgatcgtcc cgagtgtcgg gccagagaac
     2341 tccgtcgtga tcccctttac tatctcctgg tgggcgtggt gcgatgtcga tatgtcgaac
     2401 ggagacttcc tgagcttgat tgtgaggatg atggcaagcc cgaggaagac cccgggaagg
     2461 tagaggatag ccggaacggc cactgcggcg atatcggcaa cgtagaaact gtttgcgacc
     2521 atgtagaggc cgaccgccgc aaggatgacc atcggctcgt aggccatcac ctggatcagc
     2581 tcgcgctccg ccccgatgtg gctgtagggt gagtgagcag catatgcccc gagaacaagg
     2641 aagacgtggg cgagtgtaaa ggcaaagatg gtcagcagca ggtcgccccc cgcaaagaag
     2701 agcgcgccgg caagggctat gaagaccagg taagcaagaa cgtagaagtt ctgcgccgtg
     2761 gtgacgacga cgcgctcctt ctcgaaaagt ttgccgacat cgtagaacgg ctgcaggaga
     2821 ggcgggccga ctctcccctg catcctggcg gttatcttac ggtcaattcc cgcgatgaga
     2881 ccgcccgcca ggggcgccag gataaggaat aagaccgctc ctataagcca tgcccatgtc
     2941 atagcgccac ccccgcgagg atccaggaga tgattataag cgctgtgcag accgccaatc
     3001 ctggacggaa gagttttgcc tcgccgaagt agtcgttcag gtagtagttc ctggtctggg
     3061 cctcccgggt gaggccaaga gagcccgcaa agcggcggtc ctccgtcgcc ggtctcccgc
     3121 ccatgtaagc ggggaggtgt tttgcgctcc tcttgaagag caggaacgag atcggcagca
     3181 ggagaagcag ggccatcatc aggagcatga tcgcgacgtt cgcctgcgcc atgcgtgccg
     3241 tagccccgta gatggcaagg acgtatggtt caatcagcac agatgagatc agcgggaata
     3301 ggagggtggc cgctaccacg agacccgtga ggatgtagag gggtgcccag ggctcctcca
     3361 ggataccttt ctccacccgc tcctgctcac gggtgaccgc aacaagtttg cccatccact
     3421 tcgcccagaa aaagactgtg acagcgcttc cgtaggcaag gatggctatg aagatgagcc
     3481 cgaacggggc ctggacaaaa gcctcgatcg cagcccattt cgagatcagc atcccgaaag
     3541 gcgcaaggaa cataccggcc atcccgatga acatcatcac agccatacgg ggcatccgga
     3601 cgatcagacc ctccatatcc tcgatatccc tgctgccgat cctgtgctcg atcatccctg
     3661 taccgaggaa gaggagcgcc tttgcgatgg cgtggaatat gatcaggagt atcgcagccc
     3721 atacaagcat gtaggttcca acacccgcac atgcggctat aaggccgagg tttgcgatcg
     3781 tcgagtaggc gagaacccgt ttcgcgttgc tctgcgatat cgcgatcgca gacgcgagga
     3841 cgaatgtcag gccgcccacg agccctatgg agaaccctgt aagcgttccc gcaaagaccg
     3901 gtgcgaacct gaccaggatg tatacaccgg ccttgaccat ggtgctcgag tggaggaggg
     3961 ccgagacggg ggtcggcgcc accatcgccc cgagcagcca ggaggagaac ggcatcagtg
     4021 cggcctttgt gatcccggca aacccgatca gcgccgccgg gagcaggatg accgccgcat
     4081 cgccggatga gagcaccccg gcaaggtcga tacccccact caaaggttcg gtggcggcaa
     4141 ggatgatgag cgctgcaacg aacgctattc cgccaagcag gttcataacc agcgccagaa
     4201 aagcgttctt agttgcctcc tcggtctccg cgtagccgat cagcaggaac gacgttatcg
     4261 ttgtgatctc ccagaagaag aagacccaca tcaggttgtt ggagaagacc aggccaaaca
     4321 tcgcagccag gaagatgaat atgacaaaga agaacgtcct ctgccggtca cgcacctccg
     4381 gatggtgatg gtggtaagtc tccatgtaac tgaccgagta gacggctatg agactcccga
     4441 tgatcccgat gatcagggcc atgatgatgg agaactggtc gacgaagagg ttgttgacca
     4501 catgaaggct cccagaatac gtcgtttcaa gatataccac cagagccgcc tggacggctg
     4561 ccagcgcgat tgccaggtag ttcttgaact ttacacccat gtagatgatg aagagcgcaa
     4621 gccccatctc gatgaccacc atgagcgggt cgaccgtccc tgagggcacg gcaaagtaga
     4681 ccgcgcccgc agagatatgc cgcaacagca ggtatatcgt cgccccgccg atggcaagag
     4741 cggagatgac caccaccacg tagcgcaatg ccgttcgttt tacgaacaac aaaagacatg
     4801 cggcgatgat gggaaataag ataaggaaga gcagcgtctg cactattgtc cctcgcaaag
     4861 gaa
//
LOCUS       DAOSZX010000104         4734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_14932_length_4734_cov_20.807224, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000104 DAOSZX010000000
VERSION     DAOSZX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14932_length_4734_cov_20.807224"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2007
                     /locus_tag="PK196_07345"
     CDS             64..2007
                     /locus_tag="PK196_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HON41267.1"
                     /translation="MEMSKQTIGLIQTAVSEDPDHNLKHALDMAGSAISKGARIICLP
                     ELYRAPYFPQYEDADASAYAETIPGASTEAFSALAREHGAVIILPIYERAASGEHYNS
                     AVVIDADGRVLFPAYRKVHIHQDPPIYDKKYYHAGDRYRLYDTRYGRIAVLISYDQWF
                     PEAARAVALRGAEMIFYPSAIGRISGESSSGIDWREAWETVQRGHAIANGLHIAAVNR
                     VGEDGCIRFFGSSFIADAFGNVIARASEADEEVLIAEVDLSMNEAARETWNLFRNRRP
                     ETYRALVRKFPPGKTPQALGYRMPAEWEPHDAVWLAWPYDREMLPDLAAVEWNYVEII
                     AALRRSETVNLLVTGEQMRTRVGGILLEGGIDASGVRFHILDYADVWLRDYGPTFVVN
                     RRTESLAMVHWNFNAWGEKYPDLKKDSEVPLMMNRGLGLPIFSPGIVLEGGSVEVNGA
                     GTAIATESCLLNPNRNPRITKEDIEAYLEAYLGVSHVIWLKEGIAGDETGGHVDNIAR
                     FVNPTTVLCVIEDDRDDENYDPLKENYEILRSSTDQDGKPLTVIPLPTPGRVGGEVRL
                     PASYANFYIGNTVVLVPTFKHPNDAAALARIRQAFPGRKVIGIDCTALVEGMGGVHCI
                     TLQQPSISSGVTGHRAESPSRKK"
     gene            complement(2104..2976)
                     /locus_tag="PK196_07350"
     CDS             complement(2104..2976)
                     /locus_tag="PK196_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON41268.1"
                     /translation="MTPDRSAAGLITPVQNTWCPGCGNFSIQHMMRSAIAEISEEEGI
                     PIEKFVLLGGIGCHGKLMDYLNVNSLYTIHGRSIPAATGIRLANRDLRVICHVGDGDI
                     YAEGLDHLIFAAKRNTDITVIVHDNRVYGLTTGQYTPTSPTGFRGRSTPRGVAEHPMN
                     PLEVMLAAGATHIARGYTRKTRHLKDVFKEAIMHRGFSFVDVLQICATYSNLTDYYDA
                     HVYEMQDGDLDTGRFESALQKIREWDYDRDAPIAIGTFYSIERPLYEEGFSVPPSGLE
                     ERRGRVRRLVEGWR"
     gene            complement(2973..4631)
                     /locus_tag="PK196_07355"
     CDS             complement(2973..4631)
                     /locus_tag="PK196_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HON41269.1"
                     /translation="MQEYSLLIGGKAGEGINTAGLSIAGLFSRLGYRVYMYFDYPSLI
                     RGGHNFAIIRAADRPIGAHRTRVDVLLALDRKTIETHKERIDGKTTVIYDSSQVREET
                     GYGLPLDDIIAEEKAPPITRNSAMLGALARVAGIDREMLEDVLRAAVPEKHLDSNLRV
                     AARGYSAVENVFSVKPLDAPALPVLTGNEAAGLGLVYGGLDTYVAYPMTPSSGVLHFL
                     AGRAEDLGIRVIHPENEISVILMALGLAYAGKRAAVGTSGGGFCLMTEGLSFAGMSEM
                     PVTIVLSQRPGPSTGVPTYTAQSDLHFALNAGQGEFPRLVVAPGDPHEACAWSAAALM
                     LSWRYQVPAIILMDKTLAEGACSFDLDASTPPRDHEPLLEDGEGEYRRYRRTEDGVSP
                     LAFPGMEGVTVKANSYAHDERGFTTEKAEEIRALQEKLLRKAGSLKAELGGYQTVKTY
                     GAPDAEKTIICWGSQKWVCIEAAAGVDARVVQPVVLAPFPVEAWSKAMAGAGEVVCVE
                     NNATGQAARLLREHGFDPGRPILKYDGRPFAVDELTARLEEVFV"
BASE COUNT          929 a         1409 c         1446 g          950 t
ORIGIN      
        1 gataaacgct gtgttgcggc gattgttata agacgctgga gcgtcctacg gtatgaggag
       61 atgatggaga tgagcaagca aacgatcggc ctcatccaga cagcggtaag cgaagacccc
      121 gatcacaacc tcaagcacgc cctggatatg gcaggctcgg cgatctcaaa gggtgcgcgg
      181 atcatctgcc ttccggaact ctatcgtgcc ccttacttcc ctcagtatga ggatgcggac
      241 gcctcggcct acgccgagac gatccccggc gcttcgacag aggcgttctc ggcacttgcg
      301 cgggagcatg gtgcggtgat cattctgccg atctacgagc gggccgcgtc cggagaacac
      361 tacaactccg cagtggtgat cgatgctgac ggtcgggtgc tcttccctgc ctaccggaag
      421 gtccacatcc atcaggaccc gcccatctac gataaaaaat actaccacgc cggcgaccgc
      481 tacaggctct atgacacccg atatggtcgg attgccgtgc tcatctcata tgaccagtgg
      541 ttccccgagg ctgcaagggc ggttgcgctg cggggcgccg agatgatctt ctacccgtct
      601 gcaatcggcc ggatctccgg tgagagttct tctggcatcg actggcgtga ggcttgggaa
      661 accgtccagc gcggtcatgc gatagcaaac ggtcttcaca tcgccgctgt aaaccgggtg
      721 ggcgaggatg ggtgtatccg gttcttcggg agttcgttca tcgcagatgc gtttggaaac
      781 gtcattgccc gcgcgagcga ggccgatgag gaggttctca tcgcagaggt tgacctctcg
      841 atgaatgagg ccgcccggga aacctggaat ttattccgga accggaggcc ggagacctac
      901 agggcgctcg tccggaagtt cccgccgggc aagacgccgc aggcgctggg ttaccggatg
      961 cctgcagagt gggagccgca cgatgctgtc tggctcgcct ggccttatga ccgggagatg
     1021 ctccccgacc ttgccgcggt ggagtggaac tatgttgaga tcattgcagc gctccgccgc
     1081 tcggagactg tcaacctcct tgtaaccggg gagcagatgc gtacccgtgt tggggggata
     1141 ctccttgagg gcggcatcga tgcgtccggt gtgcggttcc acatactcga ttatgccgat
     1201 gtctggctcc gcgattatgg gccgacattt gtggtcaacc gcaggacaga gagccttgcg
     1261 atggtgcact ggaacttcaa cgcctgggga gagaagtatc ctgacctcaa gaaggattca
     1321 gaggtcccgc tcatgatgaa ccgcggcctg ggtcttccaa tattctcccc cggcatcgtg
     1381 cttgaagggg gttcggttga ggtgaacggc gccgggacgg cgatcgcgac ggagtcgtgc
     1441 ctcttaaatc cgaaccgtaa cccgcgcatt acaaaggagg atatcgaggc ctacctggag
     1501 gcctacctcg gtgtcagcca tgttatatgg ctgaaagagg ggatcgccgg cgacgaaacc
     1561 ggtggtcatg tcgataacat cgcgcgtttc gtgaacccga caaccgttct ctgtgtcatc
     1621 gaagacgaca gagatgacga gaactacgat ccgctgaagg agaactacga gatcctgcgg
     1681 tcgtcaaccg accaggacgg gaaaccctta acggtaatcc ccctcccaac accaggcagg
     1741 gtcggcggtg aagtgcggtt gccggccagt tacgcgaact tctacatagg aaacaccgtt
     1801 gtgctcgtgc cgaccttcaa acaccccaac gacgctgcag ccctggcacg gattcggcag
     1861 gctttcccgg gcaggaaggt catcgggata gactgcacgg cactggtcga ggggatgggc
     1921 ggagttcact gtataactct ccagcagcca tcaatttcgt ccggtgtcac cggccatagg
     1981 gcagaaagcc cctcccgaaa gaagtaagcg ggtcatggga gattgaagtt gtaagtcgcg
     2041 gaaagaattg gctttgatgg cagtttgccg gaaaagccgg cgggggtgta aaatgacccc
     2101 ccctcacctc cagccctcga ccaacctccg gacgcgcccg cgccgctctt caagcccgct
     2161 cggcggtacg ctgaaaccct cttcgtagag gggccgctca atcgaataga acgtcccgat
     2221 cgcgataggc gcatcccggt cgtagtccca ctccctgatc ttttgaagag cgctctcgaa
     2281 ccgaccggta tcgagatcgc catcctgcat ctcgtagaca tgggcgtcat agtagtcggt
     2341 caggttggag taggttgcac agatctggag gacatcgacg aaagagaatc cgcggtgcat
     2401 tatcgcctct ttgaagacat ccttgaggtg ccgggtcttc cgggtgtagc cccttgcgat
     2461 gtgggtggcc ccggcggcga gcatcacctc gagcgggttc atggggtgct ccgccacccc
     2521 ccgcggcgtc gaacgccccc ggaacccggt gggcgacgtc ggggtgtact gtccggtcgt
     2581 caggccgtag acccggttgt cgtggacgat cacggttata tcggtgttcc gcttggccgc
     2641 aaagatgagg tggtcaagcc cctcggcgta gatgtcaccg tcgccgacgt gacagattac
     2701 ccgcaggtca cggtttgcca ggcgtatccc ggtggctgcc gggattgaac ggccgtggat
     2761 ggtgtagagg ctgttgacgt tcaggtaatc catcagtttt ccgtggcacc cgataccgcc
     2821 aaggaggacg aacttctcga tgggtatacc ctcctcctcc gagatttctg cgatggccga
     2881 tctcatcatg tgctggatcg agaagttacc gcaaccagga caccaggtgt tctgcaccgg
     2941 agttataagt ccagcggcag accgatccgg ggtcatacaa acacctcctc gagccttgca
     3001 gtgagttcat cgaccgcaaa cggtcgacca tcgtacttca ggatgggtct gcctggatcg
     3061 aatccatgct ccctgagaag acgggcggcc tgacccgtgg cgttgttctc gacgcagacc
     3121 acctctcccg cgccggccat cgccttgctc caggcctcaa ccgggaacgg ggcaagcaca
     3181 acaggctgaa caacccgcgc gtcaaccccg gcggcggcct cgatacacac ccatttctgc
     3241 gagccccagc atatgatggt cttctccgcg tcaggtgcac cgtaggtctt cacggtctgg
     3301 tagcccccaa gttccgcttt caggcttccg gctttcctga gcagtttctc ctgcagtgca
     3361 cggatctcct ctgctttctc ggttgtaaac cccctctcgt catgggcgta gctgtttgcc
     3421 ttgaccgtca ccccttccat tcccgggaag gcaagcgggg atactccatc ctcagtccgg
     3481 cggtaacgcc ggtattcccc ctctccatcc tcgagcagcg gttcgtggtc ccgcggcgga
     3541 gttgatgcat cgagatcgaa ggagcaggca ccttcagcga gcgtcttatc catcaggatg
     3601 atcgccggga cctggtagcg ccaggagagc atcagcgccg ccgctgacca ggcgcaggct
     3661 tcatgcggat cccccggggc gacgacgagc cttggaaact ctccctggcc ggcgttcagg
     3721 gcgaagtgga ggtcgctctg cgcggtgtag gtgggcacac cggtgctcgg gccgggccgc
     3781 tgcgacagca ctatcgtcac cggcatctcc gacatcccgg caaacgaaag cccttcggtc
     3841 atcaggcaga accctccacc ggaggttccg accgctgctc ttttccctgc gtaggcaagc
     3901 ccgagagcca tcagaatgac gcttatctcg ttttccgggt ggatcaccct gatcccgaga
     3961 tcttcggcgc ggccggccag aaaatggaga acccccgatg agggtgtcat ggggtaggcg
     4021 acgtaggtat caagcccgcc gtagaccagt ccgagcccgg cggcctcgtt acccgtcagg
     4081 acagggagtg ccggggcatc cagcggtttt acggaaaaga cgttctccac cgcgctgtag
     4141 ccccgtgcag caacccggag gtttgaatcc aggtgttttt ccgggacggc tgcgcggagg
     4201 acatcttcga gcatctcccg gtcgatccct gcaacacggg caagcgctcc gagcatcgcg
     4261 gagtttctgg ttatcggggg cgctttctct tctgcaatga tatcatcaag ggggaggccg
     4321 tagcccgtct cctctctcac ctgtgaggag tcgtagatga ccgttgtctt cccgtcgatc
     4381 cgctccttat gggtctcaat ggttttacgg tcaagggcca gcagaacgtc gaccctggtg
     4441 cggtgcgccc cgattggcct gtccgcggcg cgaatgattg caaagttgtg cccgccccgg
     4501 atgagcgagg ggtaatcgaa gtacatgtag acgcggtaac cgaggcggga gaagagaccg
     4561 gcgatggaga ggcctgccgt gttgataccc tcgccagcct ttccaccgat tagcaacgag
     4621 tattcttgca tggtgttcag acctcgcata agtccgctca tggataaata ggttcctgca
     4681 ggcgtcggga gacggtgtct gagacggggt ggcaaaaata ccgcaggccg taca
//
LOCUS       DAOSZX010000105         4697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_15061_length_4697_cov_22.662430, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000105 DAOSZX010000000
VERSION     DAOSZX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_4697_cov_22.662430"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..459)
                     /gene="albA"
                     /locus_tag="PK196_07360"
     CDS             complement(184..459)
                     /gene="albA"
                     /locus_tag="PK196_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HON41270.1"
                     /translation="MIKDNTVFVGNKPVMNYVLAVVTQFNNGAEEVAIKARGKAISRA
                     VDTAEIALNRFLANVVKKEILTSTEMIDTDTGKTNVSSIEIILAHQK"
     gene            complement(473..1489)
                     /gene="asd"
                     /locus_tag="PK196_07365"
     CDS             complement(473..1489)
                     /gene="asd"
                     /locus_tag="PK196_07365"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HON41271.1"
                     /translation="MINVGVLGATGAVGQRFVQLLADHPWFNLQTLTASERSAGKPYG
                     RVVNWRLDAPYPDNVSDIVVTPTTVESLKDCDLVFSALPPEVAASLETEIADAGIAVC
                     SNARSHRMDSDVPLVIPEVNPDHLGLIDVQRDRGRDGFIVTNPNCSTIVFVLALAPLR
                     AFEFHDAHVATMQAISGAGFAGIPAMEIYDNVVPYIGSEEEKMETETLKIMGKFDGAE
                     VKPALLRVSASCHRVPVIDGHTMAVWVDVKEPVAEVKKAYATFEPPFSNLPLQPAKTI
                     ELFDQPDRPQPRLDRNRGRGMTVSIGRVREGLRFVALGHNTIRGAAGASVLNAELISS
                     RNYL"
     gene            complement(1536..1709)
                     /locus_tag="PK196_07370"
     CDS             complement(1536..1709)
                     /locus_tag="PK196_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41272.1"
                     /translation="MPAVVDTEKCTACETCVDVCPSAAITMVDGKAKVDPDLCVECET
                     CIDECPSEAISME"
     gene            complement(1773..2534)
                     /gene="dapB"
                     /locus_tag="PK196_07375"
     CDS             complement(1773..2534)
                     /gene="dapB"
                     /locus_tag="PK196_07375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HON41273.1"
                     /translation="MAIKAVISGALGRMGGTVGRIVSEAHDLELVGGIDVKKGSLFGT
                     EVVPATEIDAFLKEKKPDVLIDFSVAAAAVENIKAAARNNVALVVGTTGFSPAQRKEI
                     ESAVEGKVPAVISSNFSVGVNIFWKLVREAARQLGDYDIEVTEAHHRYKKDAPSGTAK
                     TILDILDQELGEREKVYGRVGATERKDEIGVHVIRGGDIVGDHSVLFAGNFECIEISH
                     RAYDRAVFAQGAIRATRWVVGREPRIYTMQDVLGL"
     gene            complement(2524..>3270)
                     /gene="dapA"
                     /locus_tag="PK196_07380"
     CDS             complement(2524..>3270)
                     /gene="dapA"
                     /locus_tag="PK196_07380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HON41274.1"
                     /translation="GSTGESATLTFEEHETVIAKTVEVVDGRVPVLAGTGSNSTAEAM
                     RLTRSAKDAGADGALIISPYYNKPNRSGLIKHFTKLADLDLPIVIYNVPGRTAQNLQP
                     DLVAELAEHPNIVGIKEASGDITQISRIIEETQDEDFSVLSGDDAMTLPILAIGGAGV
                     ISVAANIVPGLMVEMYESFSAGDLAHAQDLHYILSPLMRAMFIDTNPIPVKKAVELLG
                     MAAGPVRLPLDELDAARTELLKGVLANYGH"
     assembly_gap    3271..3370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3371..3785)
                     /locus_tag="PK196_07385"
     CDS             complement(<3371..3785)
                     /locus_tag="PK196_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HON41275.1"
                     /translation="MTQEEREKVIKRLGEAVGLLAERMDARLIPEVGMNIVYALPDAR
                     SREDVAGVMGRIVRLGDRVHPVGEIAFGASDHMARVVLTAMRFDPKIRSAANIRFSDE
                     IVSKLNDLLFEVCSFDRGKEPPGVQTMDWGVASCCR"
     gene            complement(3823..4446)
                     /locus_tag="PK196_07390"
     CDS             complement(3823..4446)
                     /locus_tag="PK196_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON41276.1"
                     /translation="MHVYLYPCSRARPTPLSRATDNFSRMEREEAIDLLKRYVTSQSH
                     IVHGHATAAIMRKVAEHLGEDADMWEIIGLLHDIDYDLVDGEMERHGIEGYQILIENG
                     VPRDIAEIVRRHNHMLTSGYERPIEVALQAADSASGLIIACALVKGGAIDDVTPRTVK
                     KKFKEKSFAAGCERERILMIESLMDIGTFYHLAIEGLKEARADLGLA"
     gene            4464..4646
                     /locus_tag="PK196_07395"
     CDS             4464..4646
                     /locus_tag="PK196_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41277.1"
                     /translation="MNLRVLEVLAAVVCFVLFVLLLVVLPDLMVGMEGFAYVAALAVF
                     ISSLGIAGYLIDRMIV"
BASE COUNT          913 a         1358 c         1231 g         1095 t
ORIGIN      
        1 cctggtgatt ccacccggtc cgctaccgca ggggagatcg cctcaacgga ttcacggtga
       61 gtcattggca gaggggcatg cgtgtccaac cgcagcagtc aggtatgtta cctgctcccg
      121 acggcatcca ccctcagtct gcatgccata caccgctggc gtgcaaaaaa tgttttctgt
      181 gtctcacttc tggtgagcga ggattatctc gatgctggat acgtttgtct tgccggtatc
      241 ggtgtcgatc atctcggtcg atgtgaggat ctctttcttg accacgtttg caaggaaccg
      301 gttgagtgcg atctccgccg tatcgaccgc acgtgatata gcctttccgc gtgccttgat
      361 cgcgacttct tccgctccat tgttgaactg agtgaccaca gcaagcacgt agttcatgac
      421 cggtttgtta ccaacgaata ctgtgttgtc ttttatcatt gagtaagcct ccttagaggt
      481 aattccttga agatatcagt tctgcgttga ggactgaggc acctgcagct ccacgtatgg
      541 tgttgtggcc gagcgccaca aaacgcagcc cttcgcgaac cctgccgatg gagaccgtca
      601 tgccgcgccc gcggttacgg tcaagccgcg gctgtggacg gtctggctgg tcgaaaagtt
      661 cgatagtctt tgctggctgc agcggcaggt tgctgaaggg tggctcgaat gttgcatagg
      721 ctttctttac ctcagccacc ggttccttaa cgtcgaccca tacggccatg gtatgcccgt
      781 cgattacagg aaccctgtgg cagctggcac tcaccctgag aagtgccggc ttcacctcgg
      841 cgccgtcgaa cttacccatg atcttcaacg tctcggtctc catcttctcc tcttcggagc
      901 cgatgtatgg gacaacgttg tcataaatct ccatggcggg gataccggca aacccggcac
      961 ctgagatcgc ctgcatggtt gccacatgcg catcatggaa ctcaaacgct ctcaggggtg
     1021 caagagccag cacaaagaca atcgtcgaac agttagggtt ggtcacgata aatccatcac
     1081 gcccccgatc acgctgaacg tcaatcagtc ccaggtgatc cgggttaacc tccgggatca
     1141 caagaggtac atcagaatcc atacggtgtg accgggcgtt gctgcagaca gcgatgccgg
     1201 cgtcggcgat ctcggtctcg agtgatgctg ccacctccgg aggcagcgcc gaaaagacaa
     1261 ggtcacagtc tttcaggctc tcgacggttg ttggagtgac gacgatatcg ctgacgttgt
     1321 ccgggtacgg cgcatcaagc cgccagttga ccaccctgcc ataaggtttt cctgcacttc
     1381 gttcagatgc tgtgagggtt tgaaggttaa accagggatg atccgccagc aactgaacaa
     1441 aacgctgtcc tactgcccct gtggcaccaa gcactcctac attaatcatg gataggagta
     1501 tctctggtgc tggtgatatt aaaagagttg gttttttatt ccatggatat agcttcagac
     1561 gggcactcgt cgatgcaggt ctcacactca acgcagagat caggatcgac ttttgccttc
     1621 ccgtctacca tggttatggc agcagaaggg cagacatcca cacaggtctc acaggcggtg
     1681 catttctcgg tatcaactac tgctggcaaa ctcagttcct ccaaatgtac ttattttcta
     1741 cgatgccacc caaaataaac ttcactcacc catcacagcc ccaggacatc ctgcatagtg
     1801 tatatgcgcg gttcccttcc gacaacccag cgtgtggcgc ggatagctcc ctgtgcgaag
     1861 acagcacgat cgtaggcccg gtgcgagatc tcgatacact caaagtttcc cgcaaagaga
     1921 acggaatgat caccgacaat atccccgccg cggattacat gaacgccgat ctcgtctttc
     1981 cgttcggttg caccgacgcg accgtagacc ttctcgcgtt cccccagttc ctggtccagg
     2041 atatcgagga tggtctttgc cgtaccgctc ggcgcgtctt tcttgtagcg gtgatgcgct
     2101 tccgtgacct caatatcgta gtcgcccagc tggcgggcgg cttcacgcac aagtttccag
     2161 aagatattga cgccgacgga gaagttgctc gatatcaccg ccggcacctt cccctcaacg
     2221 gcgctctcga tctcctttcg ctgtgccggc gaaaacccgg ttgtcccaac taccagtgcc
     2281 acattatttc ttgctgctgc tttgatgttc tcaaccgcag cggccgcaac ggagaagtca
     2341 atgagaacgt cgggtttctt ctccttgagg aatgcatcaa tctctgttgc cgggaccacc
     2401 tccgtcccga aaaggcttcc cttcttgacg tctatcccgc cgaccagctc gaggtcatgg
     2461 gcctcgctga ctatacggcc gaccgtgccg cccatgcgcc cgagggcgcc ggatataacc
     2521 gccttaatgg ccatagtttg caagcacccc cttcaggagt tctgtcctgg ccgcatccag
     2581 ttcgtccagc ggcagcctga ccggacctgc agccatcccg aggagttcca ccgccttctt
     2641 taccgggatt gggtttgtgt cgatgaacat tgcgcgcatg agtggcgaga ggatgtagtg
     2701 caggtcctgt gcgtgagcaa ggtcaccggc gctgaacgat tcgtacatct ccaccatcaa
     2761 ccctggcaca atgttcgcgg cgaccgatat cacgcctgcg cctccaatgg ccaggatggg
     2821 aagggtcatc gcatcgtctc cggagagaac actgaaatcc tcgtcctgcg tctcctcgat
     2881 gatacgggag atctgggtta tatcgccgct tgcctctttg atcccgacga tgtttggatg
     2941 ctctgcaagt tctgccacca ggtctggctg aaggttctgc gctgtccggc ccggaacgtt
     3001 gtatatgacg atcggtagat caaggtcagc gagttttgtg aaatgcttga tcaaccctga
     3061 acggtttggc ttgttgtagt aagggctgat gataagcgcg ccatctgcgc cagcatcctt
     3121 tgctgatcgg gtcaggcgca tggcctctgc cgtgctgtta gatcccgttc ccgcgagcac
     3181 gggcaccctg ccgtcgacaa cctcgaccgt ctttgcgata accgtctcgt gctcctcgaa
     3241 cgtgagtgtt gctgactcgc cggttgagcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn ccctgcagca ggaggctaca ccccagtcca tcgtctggac gccaggcggc
     3421 tccttccccc ggtcaaaaga gcagacctca aaaaggaggt cattgagttt ggaaacaatc
     3481 tcgtccgaga aacggatgtt tgccgcgcta cgtatcttcg gatcgaagcg catggccgtg
     3541 aggacaaccc ttgccatgtg atcgcttgca ccgaacgcga tctccccgac ggggtgaacc
     3601 cggtcaccaa gcctgacgat gcgtcccata acacctgcta catcctctct gctccgcgcg
     3661 tctggcagag catagactat attcattcca acctcgggga taagccgcgc gtccatgcgt
     3721 tcggcgagca gccctacggc ctctcccaat ctcttgataa ccttctcgcg ttcttcctgt
     3781 gtcatatcga gatcaccaat atgaatcttg tctcgattgc gttcatgcaa gcccgagatc
     3841 agcacgcgcc tccttgagcc cctcgatcgc gaggtggtag aaggtcccaa tgtccatgag
     3901 cgactcgatc atcaggatcc gttcgcgctc gcaaccggcg gcaaaagact tctctttgaa
     3961 cttcttcttg accgtcctcg gtgtgacatc gtcgatcgcc ccgcctttta caagggcgca
     4021 ggcgatgatg aggcccgagg cgctgtcagc agcctggagc gcaacctcga tggggcgttc
     4081 gtagcctgat gtaagcatgt ggttgtggcg cctcacaatc tccgctatat ccctggggac
     4141 gccgttctcg atcaggatct ggtagccctc gatcccatgg cgctccatct ccccatcaac
     4201 aagatcgtag tcgatgtcgt gcaggagacc gatgatctcc cacatatccg catcttcacc
     4261 gaggtgctcc gcgaccttcc tcatgatggc tgccgtggcg tggccgtgta cgatgtgtga
     4321 ctgcgacgtc acgtagcgct tgagcaggtc aatggcctcc tctctctcca tgcgggagaa
     4381 gttgtcggtc gccctggata agggtgtggg tcgcgcccgg ctgcagggat agagatatac
     4441 atgcatctgt tccacctcaa tacatgaatc tacgggttct tgaagtgctg gcggcagtgg
     4501 tgtgctttgt gctctttgtc ctgctgctgg tggtgctgcc cgatcttatg gtgggaatgg
     4561 aagggtttgc ctatgttgcg gcgctggcgg tctttatcag ttcactcggt attgcaggct
     4621 accttatcga cagaatgatc gtgtgaacta ccccgcgctc atcggtgcgg ggcttcctgc
     4681 ttcatggcca gcacctg
//
LOCUS       DAOSZX010000106         4618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_15353_length_4618_cov_14.212579, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000106 DAOSZX010000000
VERSION     DAOSZX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15353_length_4618_cov_14.212579"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK196_07400"
     CDS             complement(<1..259)
                     /locus_tag="PK196_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843697.1"
                     /note="allows the formation of correctly charged
                     Gln-tRNA(Gln) through the transamidation of misacylated
                     Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA
                     synthetase; forms a heterotetramer of GatD2E2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit D"
                     /protein_id="HON41278.1"
                     /translation="MIDTLRTGDLVRYTVNGKTALTGVYITERDGMAVIKLESGYNIG
                     ASPEKIEFIERPAARPPAAAAAVAQNPDLPELAILSTGGTIA"
     gene            complement(256..1734)
                     /gene="argH"
                     /locus_tag="PK196_07405"
     CDS             complement(256..1734)
                     /gene="argH"
                     /locus_tag="PK196_07405"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HON41279.1"
                     /translation="MRTDQIREGRLAGERSHDIERFLASMDADRWIARADLLVDMAHL
                     LGLRRQGIIEDAPARALMAALLDFYDHGLPEAAFDERFEDVHAGKEACLIERVGEDVG
                     GRLHIGRSRNDEVATCIRIRLKEEILGLLSSLTDLRRTLLDIAAAHTESVMPGFTHLQ
                     HAQPTTLAHHLLGYEQGFSRDTSRLREAYARLDVSPLGSAAFASTGYPLDREYTAGLL
                     GFPRPAPNSMDAVASRDFVIEILADTAACMTTVSRLCEDLVLWSTSFFGFVRLDDAYC
                     SSSSIMPQKKNPDVAEIMRAKAGTVAGSLAAAITITKALPMSYNRDLQEVTPHLWRGI
                     EAARESVPLLSGMIATAVFDTERMAAEAGRGFSTATDLADALVRDYGLPFRTAHRIVG
                     RAVRHGSLDLDTLEAAAREAAGISVLEMGLTQEKIDTVLDPRHAVAARKIIGGPAPET
                     AAAQIEVQRETLALEAAWVNERETALSRAFEDLVLEARRFAE"
     assembly_gap    1768..1867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1941..2921
                     /locus_tag="PK196_07410"
     CDS             1941..2921
                     /locus_tag="PK196_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON41280.1"
                     /translation="MRSHAAVILAVAMVAAFVLIAGCTGTGDATHLRIGYQPSTHQIA
                     HVTAMEKGWWQEDLAPLGITEVTDYEFGTGAPEMQAMLAGDLDIAYVGAAPFVAAVSS
                     GLDAKIVAAVQTQGSDLVLRNEIPYSGPADLVGRKIATYPPGTIQDTLLRTWLEENGV
                     DPESVEIVPMDPGPATTAISAGQVDGVFLPHPSPAIIAASGAGRTVVKSGEMRKDHAC
                     CVLVASGPLIRDHPEIVEQVVKTHIRATEYNLEHQDEAAAIYAARSGQNVDIVKASFE
                     DWDGTWTADPHLIASSVVEYTELQYELGYIKRALTEDDLFDFTFYDEAVA"
     gene            3059..3838
                     /locus_tag="PK196_07415"
     CDS             3059..3838
                     /locus_tag="PK196_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41281.1"
                     /translation="MKRQKQKQLLGIAALIVAAAIWQFVAEYIVGRSFILPSVTDVAA
                     AFVNLLASGTLLVDIGVSFKHFGIGLLAAIFLGVPIGILMGWFRVADHLLDPIIEILR
                     PIPPLAWIPFAIIWFGLTAGAAGFVIFVGAFFPILTATYTAFRSVPKVYVEAGKVLGC
                     DTSFELIRHVALPSAIPSIASGIRISMGVGWMCLVAAEMFGVSRNGLGYQLWHNYYLH
                     QMPEVVVYMLILGFAGLIIDHIFRHYVDQRLLRWHEGEVAS"
     gene            3835..4596
                     /locus_tag="PK196_07420"
     CDS             3835..4596
                     /locus_tag="PK196_07420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41282.1"
                     /translation="MSGVIIENLSKSFPKEDGDVIWALKNINLEIQDKEFVCLVGPSG
                     CGKTTLLRIIAGLETPTAGRVIVDGNEVTGPDPRRGMVFQEYSLFPWRKVIDNIAFGL
                     EMRGVEKEERRKTAERYLEMVGLSQFRDAYPYELSGGMRQRVAIARALANDPDVLLMD
                     EPFGALDAQTRNRMQGELLSIWEKTKKTIIFITHSVDEAVYLSDRIVVLTPRPGSIRE
                     IVEIPWPRPRDRTSSEFAEVRRKVLRMIEDTGNSQ"
BASE COUNT          855 a         1387 c         1398 g          878 t
ORIGIN      
        1 ttgcgatcgt cccgccggtg gagaggatgg caagttccgg gagatcgggg ttctgcgcga
       61 cggccgcggc ggctgccgga ggcctggcgg ccgggcgctc gataaactcg atcttttcag
      121 gcgacgctcc tatgttgtag ccgctctcca gttttatgac ggccatgccg tccctttcgg
      181 tgatatagac ccctgtgagc gcggtttttc cgtttaccgt ataccgcacc aggtctcctg
      241 tcctgagagt atcgatcatt ctgcaaacct ccgtgcctca aggacgaggt cttcaaaagc
      301 acgcgataac gccgtctctc tctcattcac ccacgccgcc tccagggcca gggtctcacg
      361 ctgcacctct atctgcgccg cggcagtctc cggcgccggg ccgccgatga tctttctggc
      421 tgcaacggcg tggcggggat cgaggacggt atcgatcttc tcctgcgtga gccccatctc
      481 cagaaccgat atcccggccg cttcccttgc ggcggcctcg agcgtatcga ggtcgagcga
      541 accgtgcctg accgcccggc cgacgatccg gtgggcggtc ctgaacggga ggccgtagtc
      601 cctgacaagg gcgtcggcaa ggtccgtggc ggtggagaac cctctccccg cctccgctgc
      661 catcctctcc gtatcaaaga ccgcggtggc tatcatcccg gagaggagcg ggacgctctc
      721 cctcgcggcc tcaataccgc gccagaggtg cggcgtcacc tcctggaggt cgcggttgta
      781 gctcattggg agggcctttg tgatcgtgat cgccgctgca agcgaacccg ctaccgttcc
      841 ggcctttgcc cgcatgatct cggcgacgtc ggggttcttc ttctggggca tgatcgatga
      901 ggaggagcag taggcatcgt cgagccggac gaacccgaaa aacgaggtgc tccagaggac
      961 gagatcctcg cagagacggc ttaccgtcgt catgcaggcg gcggtgtcgg cgagaatctc
     1021 gatcacgaag tcccgcgagg cgacggcgtc catgctgttt ggcgcggggc gcgggaaccc
     1081 caggagtccg gcggtgtatt cgcggtcaag ggggtagccg gtcgatgcaa acgccgctga
     1141 accgagcggc gagacgtcca gccgggcata cgcctcccgg agcctggagg tgtccctgga
     1201 gaaaccctgc tcgtagccga gaaggtggtg ggcgagggtg gtgggctggg cgtgctggag
     1261 gtgtgtgaat cccggcatca cgctctcggt gtgagcggcg gcaatatcga gcagtgtccg
     1321 ccgcaggtcg gtgagagagg agagaaggcc caggatctcc tctttgagcc gaatccttat
     1381 gcaggtggct acctcgtcgt ttcgagaccg gccgatgtgg agacgcccgc cgacatcctc
     1441 gcccacgcgc tcgatgaggc aggcctcttt gccggcatgg acgtcctcga accgttcgtc
     1501 gaacgccgcc tccgggagac cgtgatcgta gaaatccagg agcgctgcca tcagcgcacg
     1561 cgccggggcg tcctcgatta tcccctgccg ccgcagcccg agcaggtgcg ccatatccac
     1621 aagcaggtct gcccttgcta tccagcggtc agcgtccatc gatgcaagga agcgctcgat
     1681 atcgtgcgac cgttcgcctg caagacgacc ctctcgaatc tgatccgtcc gcatcctatc
     1741 aacagtcctg tctgtagcgt ttggttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnttg tgcgattttc gacaatacta tatacagaca gcgtgaagag tacccatcca
     1921 gtatacggat ggaggttgat atgagatctc atgcagcagt tatcctggcc gtggccatgg
     1981 ttgcggcttt cgtgcttatc gccgggtgca ccggcaccgg cgacgcaacg caccttcgta
     2041 taggctacca gccgagcacc caccagatcg cccacgtcac cgcgatggag aagggatggt
     2101 ggcaggaaga cctggcacca ctcggcatca cagaggtgac cgactacgag ttcgggacag
     2161 gcgcacccga gatgcaggcg atgcttgccg gcgacctgga tatcgcctac gtcggcgccg
     2221 ccccgtttgt tgccgccgtc agcagcgggc ttgacgcaaa gatcgttgcc gctgtccaga
     2281 cccagggctc tgacctcgtg ctcaggaacg agatccccta ctctggcccc gccgaccttg
     2341 tcggcaggaa gattgccacc tacccaccag gaacgatcca ggacaccctt ctgcgaacct
     2401 ggcttgaaga gaacggcgtc gaccccgaga gtgtcgaaat tgtccccatg gacccgggtc
     2461 ctgccaccac cgccatctcc gccggccagg ttgacggtgt cttcctgccc cacccctcgc
     2521 cagcgatcat agcggcgagc ggcgcgggga gaaccgtggt gaagtcgggc gagatgcgaa
     2581 aggaccatgc atgctgtgtc ctggttgcaa gcggcccgct gatccgggat caccccgaga
     2641 tcgtcgagca ggtcgtaaag acgcacatca gggcgacaga atacaacctc gaacaccagg
     2701 acgaggctgc agccatctac gcagcaagga gcggacagaa cgtcgatata gtgaaggcgt
     2761 cctttgagga ctgggacggc acctggacgg ccgacccgca cctgatcgca tcctcggttg
     2821 tcgagtacac ggaactccag tatgaactcg gctacatcaa gagagcgctc acagaggatg
     2881 acctcttcga cttcacgttc tacgatgagg ctgtggcata aacccggccc cctaccacct
     2941 ttttccttcc aaaagtatct tctatcgacg tgccgatagg atgagggata aagacgtctg
     3001 agcgggacgc catttgcggc cccgccggca cgaccatcct gcgaggcgga acagagccat
     3061 gaaacggcag aaacagaaac aactccttgg catcgcagcg ctcatcgtcg ctgcggcgat
     3121 ctggcagttt gtcgccgagt acatcgtggg gcgatcattc atccttccaa gtgtcaccga
     3181 tgtcgctgcg gcgttcgtaa acctcctcgc ctccgggacg ctcctggttg acatcggggt
     3241 gagttttaag cattttggaa taggactcct tgcggcgatc ttccttggcg ttcccatcgg
     3301 gatcctgatg ggatggtttc gggtggctga ccacctgctt gacccgatca tcgagatcct
     3361 ccgacccatc ccgccgcttg cctggatccc gtttgccatc atatggttcg ggctgaccgc
     3421 cggggcagcc ggttttgtca tctttgtggg ggcgttcttc cccatcctga cagcgaccta
     3481 caccgctttc cggtcggtcc cgaaggtgta tgttgaggcg ggcaaggtgc tcggctgcga
     3541 cacatcgttt gagttgatcc gtcacgtcgc cctcccatcg gcgatcccat ccatagcctc
     3601 cgggatccgg atatcgatgg gggttggctg gatgtgtctt gtggcggcag agatgtttgg
     3661 agtctccaga aacggcctcg gctaccagct ctggcacaac tattaccttc accagatgcc
     3721 ggaggtcgtt gtttacatgc tgatcctcgg gtttgccggg ctcatcatag accacatctt
     3781 caggcactac gttgaccagc ggctcctgcg gtggcatgaa ggcgaggtgg cctcatgagt
     3841 ggagtcatca tagagaacct cagcaagtcc ttcccgaagg aggacggcga cgtcatatgg
     3901 gcgctaaaga atatcaacct ggagatccag gataaggagt ttgtctgcct ggtggggccg
     3961 tcaggctgcg ggaaaacgac gcttctgcgg atcatcgccg ggctcgagac cccgaccgcc
     4021 gggagggtta tcgtcgacgg caacgaggtc accggccctg acccgaggcg tggcatggta
     4081 ttccaggagt actcgctctt cccctggcgt aaggtcatcg acaacatcgc cttcggcctt
     4141 gagatgaggg gcgtggaaaa ggaggaacgg cggaagacag cggaacgcta ccttgagatg
     4201 gtcgggctct cgcagttccg ggacgcctac ccctacgaac tctccggcgg gatgcggcag
     4261 cgtgtggcga tagcgcgggc gcttgcaaac gaccccgacg ttctcctgat ggacgaaccg
     4321 ttcggggcgc tggatgccca gacccggaac cggatgcagg gagaactcct ctccatatgg
     4381 gagaagacaa agaagacgat catctttatc acccacagcg tggatgaggc ggtctacctc
     4441 tctgaccgga tcgtagtcct cacaccccgc cccgggtcga tccgggagat agtcgagatc
     4501 ccctggccgc gaccgcgtga ccgcaccagc agcgagtttg ccgaggttcg ccggaaggtg
     4561 ctccggatga tcgaggatac aggaaacagc cagtaaggct atctgtgacc cggtctgg
//
LOCUS       DAOSZX010000107         4573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_15546_length_4573_cov_13.208499, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000107 DAOSZX010000000
VERSION     DAOSZX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15546_length_4573_cov_13.208499"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK196_07425"
     CDS             complement(<1..594)
                     /locus_tag="PK196_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41283.1"
                     /translation="MDAVLLIISITFLYTSMDCSRNGRTRILQVTKRWEKLCYGSAVN
                     GERRYDQSDRRVSSSRAALIKVMPVSGNWNSGTPWCMSRGSQTSRPGRGSGICGDANA
                     VLWCPGRGGIVNEGFCPATMALTGQEEVWATVKLVFIPDRQHLLLRTGEAETFSLLSH
                     ANVIRGQAPSMDRGVFGMRTAERDVRHALERLKTVEGF"
     gene            complement(903..4031)
                     /locus_tag="PK196_07430"
     CDS             complement(903..4031)
                     /locus_tag="PK196_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HON41284.1"
                     /translation="MPDQVACPDAIRLLVERFDYHRQAYHRGQYNETQLRREFIDPFF
                     RALGWDVDNQKGHSEAYKEVAHEDPIRIRGQTNFLDYSFRIGGVRKFIVEAKKPSVKI
                     RDDTESALQLRRYAWNAGLKLSILTNFEEFAVYDCTKPIRKGDTAGTARIGYITYREY
                     PEQWGWIAKIFSRECILKGAFDKFADSETDKKGTMGVDEAFLADIEAWRDILARNIAL
                     RNPEIAVDELNLAVQRTIDRIIFLRICEDRGIEPYGTLQELLSGDRVYQRLCDLFRHA
                     DDRYNSGLFHFEEEPGRNEPPDTLTLSLAIDDDVLKTIIRRLYYPESPYEFSAIPAEI
                     LGQIYEQFLGKVIRLTEGHRAKVEEKPEVRKAGGVFYTPTYIVDYIVRQTVGEVVRGK
                     TPKEVSDITILDPACGSGSFLLGAYQFLLNWHRDWYIEHLVPVIKEKGATSSEVRALL
                     PPAREGAQRRGRRGASGQHLPIYKAANGTDSRVRSDWRLTTAERKRILLNNIYGVDID
                     QQAVEVTKLSLLLKVLEDESEETVSRQLTLFAERALPSLHDNIKCGNSLVASDIYDNL
                     EVLPGIEERKRINAFDWEREFAGVMQRGGFDVVIGNPPYVRQETLGHEFKEYAKQHYA
                     VYHGVADLYTYFIEKGVSLLRPGGQFSYIVANKWLRANYGKPLRRWLKERRIEEIVDF
                     GDLPVFQGVTTYPCIIRVSHGKPRETFPVTEVASLDFSDLSEYVRESSFLVRQDDLDE
                     EGWSLVDEQTGALLKKILGAGVPLGEYVQGKIYRGVLTGLNKAFVIDAETRDRLIAED
                     PRSAEVIKPFLAGRDVKRYAPLEADKFLIFTRRGIEINDYPAIKEYLKQFKKELMPRP
                     KGWKGGKWEGRKPGSYQWYEIQDTVDYYEGFEGPKIIFPDIAERPQFTIDAEGGFYNA
                     NTCYNVFINDTFLLGILNSSLINFYYRNVAAVYRGGYLRFFSQYVEMLPIRPIDPSDP
                     ADVARHDRMVSLVEKMLDLNRRLAGARMGQDKIFISREIEATDARIDALVYELYGLSE
                     EEIALIERAVRS"
BASE COUNT          945 a         1453 c         1178 g          997 t
ORIGIN      
        1 gaatccctcg acggtcttaa gccgctcaag tgcgtgccgg acgtcccgct ccgctgttct
       61 catcccgaaa actcccctgt ccatagatgg ggcctgtccc ctaataacgt ttgcatggga
      121 gagcaggctg aatgtttctg cctcgccggt gcggaggaga agatgctggc ggtcagggat
      181 gaagaccaac ttcaccgtcg cccagacttc ctcctgcccg gtcaatgcca tggttgccgg
      241 gcagaacccc tcgttaacga taccgccacg accgggacac cacagcactg cgttggcatc
      301 tccacaaatc cctgaaccgc gcccgggccg actcgtttgc gaacccctgc tcatgcacca
      361 gggcgtccca gagttccaat tgccggaaac cggcataacc tttatgagtg cggctctgga
      421 ggaggacact ctcctatcag actggtcgta tcgcctctct ccattgactg cagagccata
      481 acagagcttc tcccatctct ttgttacttg caggattctg gtccttccgt ttctggagca
      541 atccatggac gtgtaaagga atgtgataga aattataagt agaacagcgt ccatcccatt
      601 gtgtgtatgc ttccttgaag cgtctgcaag aattcgtggg gtctccccat cctctggttg
      661 acggccggat gatagagccc ctgcatgccc ggtgtgttag cgcggagttt gtgccggtga
      721 tgagtgcagc atcggtaaac gctgaaaata gtcatctttt gtctgactac agagttgcag
      781 atctctcaaa tcgtgatccg atggttctcc ctctcaggaa aatccatgtt catgcctacc
      841 ggtacacgat ccctccacca ggccggcatc gcttctctcc gtgtcccctc gacataccgg
      901 cttcatgacc gaacagcccg ttcaatcagt gcgatctctt cctctgagag tccgtagagc
      961 tcgtagacca gcgcgtcaat cctcgcatcg gtcgcctcga tctcgcggct gatgaagatt
     1021 ttatcctgcc ccatcctcgc cccggcaagc ctccggttca ggtcgagcat cttctcaacg
     1081 agggagacca tgcggtcgtg gcgggcgacg tcggcagggt cggaggggtc gatggggcgg
     1141 attggcagca tttccacgta ctgtgagaaa aagcggagat accctcctcg atacaccgcc
     1201 gccacattcc gatagtagaa gttgatcaaa cttgaattga ggatgccaag caggaacgtg
     1261 tcattgatga atacattata gcaggtattg gcgttgtaga acccgccctc tgcatcgatg
     1321 gtgaactggg ggcgctcggc aatgtcaggg aaaataatct tgggcccctc gaaaccttca
     1381 taataatcaa ccgtatcctg aatctcatac cactggtacg accccggttt cctcccttcc
     1441 cacttgccgc ctttccatcc ttttggtctt ggcatcagtt ccttcttgaa ctgcttgagg
     1501 tactccttaa tcgcaggata atcgttgatc tcgatgcccc ttcgtgtaaa tatcaagaac
     1561 ttatccgcct ccagcggcgc ataccgcttc acatcccgcc cggcgaggaa cggcttgatc
     1621 acctccgcgc tcctcgggtc ctctgcaatc agcctatccc gggtctccgc atcgatgacg
     1681 aacgccttat tcagcccggt aaggacgcca cggtagatct tcccctgcac gtactcccca
     1741 agcggcaccc cggcgcccag gatcttcttc agcagagcgc cggtctgctc atccacaagc
     1801 gaccacccct cctcatccag gtcgtcctgc ctgaccagga aactcgactc cctgacatac
     1861 tccgagaggt cagagaaatc cagcgacgca acctccgtga ccgggaacgt ctcacgcggt
     1921 tttccgtgcg ataccctgat gatacagggg tacgtcgtca ccccctgaaa caccggcaga
     1981 tccccgaagt ccacgatctc ctcgatcctc ctctccttca gccaccggcg gagaggtttc
     2041 ccatagttcg cccgcaacca tttgttcgcg acgatgtagc tgaactgccc gccgggccga
     2101 agcagggaga cacccttctc gatgaagtag gtatagagat cggcaacccc gtggtagacc
     2161 gcatagtgct gcttcgcata ctccttgaac tcatgcccca gcgtctcctg cctgacgtaa
     2221 ggcgggtttc cgatcacaac atcgaacccg ccgcgctgca taaccccggc aaactccctc
     2281 tcccagtcaa acgcattgat ccgcttgcgt tcctcgatcc cgggcagaac ctcaagatta
     2341 tcgtagatat cagaagcgac aagcgagttc ccgcacttga tgttgtcatg caggctcggg
     2401 agcgcacgct cggcaaacag ggtcagctgc cttgataccg tctcctcgct ctcgtcctca
     2461 aggaccttaa ggaggaggga gagtttcgtc acctcgaccg cctgctggtc gatatccacc
     2521 ccatagatgt tattgaggag gatccgtttc cgctcggccg tggtcagtct ccagtcgctc
     2581 ctcacccgtg agtcggtgcc gttcgccgcc ttatagatcg ggaggtgctg cccggatgcg
     2641 ccccgccgcc cgcgccgctg cgccccctcc cgtgcaggcg gaagaagcgc cctgacctcg
     2701 gacgaggtcg cccccttctc cttgatcacc gggaccagat gctcgatgta ccagtccctg
     2761 tgccagttca gcaagaactg gtatgcgcca agcaggaaag agccagaacc gcaagcagga
     2821 tccagtatcg tgatatctga aacctccttc ggcgtcttcc cgcgcaccac ctcacccacc
     2881 gtctgcctaa cgatgtaatc gacgatataa gtcggcgtgt agaagacgcc ccccgccttg
     2941 cgcacctccg gtttctcctc caccttcgca cgatgcccct ctgtcaggcg gatgacctta
     3001 ccgaggaact gctcatagat ctgccccagg atctccgcgg ggatagccga gaactcatac
     3061 gggctctcgg gatagtagag gcggcggatg atcgtcttca gcacatcatc atcgatggca
     3121 agcgagagcg tcagcgtatc aggcggctcg ttccgcccgg gctcctcctc aaagtggaac
     3181 aaccccgagt tataccggtc gtcggcatga cggaagagat cacagagacg ctgataaacc
     3241 cggtcgccgg aaagcagttc ctgcaacgtc ccatacggct cgatcccacg gtcctcacag
     3301 atccgcagaa agatgatccg gtcgatcgtc cgctggacag cgaggttcag ttcgtccaca
     3361 gcgatctcgg ggttgcgaag cgcgatattt cgggcgagaa tgtctcgcca ggcctcgata
     3421 tcggcgagaa acgcctcgtc gacacccatg gttcccttct tatcggtctc agaatcggca
     3481 aatttgtcga acgcaccctt gaggatacac tcccgggaga agatcttcgc gatccatccc
     3541 cactgctccg ggtactcccg gtaggtgatg tagcctatcc gggccgtccc cgctgtatcc
     3601 cccttccgga taggttttgt gcagtcatag acagcaaact cctcaaagtt cgtaagaatg
     3661 ctcagtttca gtccggcgtt ccaggcatac ctgcgcagct ggagcgccga ctccgtatcg
     3721 tccctgatct tcacggacgg cttcttcgcc tcgacgataa acttccgaac cccgcctatc
     3781 cggaacgagt agtcaaggaa gttcgtctgc ccacgtatcc tgatcggatc ctcgtgcgca
     3841 acctctttat acgcctcgga atgccctttc tggttatcga catcccaccc cagcgccctg
     3901 aagaaaggat cgatgaactc ccggcgcagc tgcgtctcgt tgtactgccc ccggtggtat
     3961 gcctggcggt gataatcaaa gcgttcgaca agcaacctga tggcgtcggg gcatgctacc
     4021 tgatcgggca ttcaagcatc gggctccctt gttaaacctt cgagcccgat gctcataaat
     4081 aggttgtttt ttttgtcgat atgggtcgca aggtccatgc aattttggca aacagtgttt
     4141 tatagggaag aacccagata ccaaaaaaaa cagatgcgag gggagcgatt tgaacgctcg
     4201 acctcctgtg aggcaaagcc cctcgcccct gcgtctatac aagtgcctgc aacggggaga
     4261 gatcggtccg aatccccggt cgcaaaggaa ctgcgggaag cagagcactc tctcacagag
     4321 ttaagtgggc aatcaaccag tttcacgccc ttcgtgccgc cgccggtatg tcgtcgtggc
     4381 tccccgtgtc gacgaagccc ctctcccggc atcggtactc taagacttca acttcgccgt
     4441 gcgcatgcgg gaaggcgcgg tttatggccg cgtttactcg gaggccaacg ccgtggggcg
     4501 gtgatggcaa cccccctccc ggattcaccg gagccgggcc gtgtattctc ccgccctaga
     4561 gcagcagcgt cgt
//
LOCUS       DAOSZX010000108         4529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_15699_length_4529_cov_18.082253, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000108 DAOSZX010000000
VERSION     DAOSZX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15699_length_4529_cov_18.082253"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..616
                     /gene="hxlB"
                     /locus_tag="PK196_07435"
     CDS             17..616
                     /gene="hxlB"
                     /locus_tag="PK196_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HON41285.1"
                     /translation="MTVHPVKKMMQMMAARIMIISDGMSDEEIDTFISEILNANRVYT
                     LGAGRSGLVAKAFAMRLMHLGFRAYVVGETVTPAMKPGDALIVFSGSGATRTVADIAE
                     TAKNIGGKVCLITSKRDSRIARIADCVVIIESQRDRVADESAEFEIRQMMGEHRSFAP
                     LGTIFETTAMVFADAIISRLMEIMSCRPEDLQCRHANIE"
     gene            626..1735
                     /locus_tag="PK196_07440"
     CDS             626..1735
                     /locus_tag="PK196_07440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HON41286.1"
                     /translation="MKEHRYADRVLGVEMSGIRRLFDAGGPDAINLGIGQPDFDTPDH
                     IKMAAIAAIREGRTGYTPNAGIPELREAICGKFARENNLTYLPEQILVTAGGSEALHL
                     AMEALVNPGDRVLVPDPGFLSYAALAVIAGGRPVGVPLDSNLHVDVEQAKELMDGARL
                     LVLNSPANPTGAVESEESIRALVEYANDRGVTVVSDEVYEHFVYEKKHVSAARFGDDV
                     ITVNAASKTYAMTGWRLGYLAAPSEDYIPQLLKVHQYAQACATSISQYAAVAAYTGDQ
                     TPVARMREEYRARRDLLYEGLTGLGFDFPRPEGAFYMFVPMGQNLIQKAIAHGVIIVP
                     GDAFGSRAPDYARISYATSRQNLTRAIARLGELMR"
     gene            1743..2780
                     /locus_tag="PK196_07445"
     CDS             1743..2780
                     /locus_tag="PK196_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HON41287.1"
                     /translation="MVKELLRKLSDAHGVSGCEGSIRDIIRAEVAGSVDEIREDSMGN
                     LIAVKRGDGFSIMLAAHMDEIGLMVQYIDEKGFIRVVPIGGWFGPVLYTQRVILHGKN
                     GPVMGVLGAKPPHMLKEEERKKEIKIEDMFVDVGAASEEEVKSLGIEIGTPITIDREF
                     ADLAGNRVTGKALDNRVGVAMLIRALQRMESPHTVYAVFTVQEEVGLKGAKVSAYSLN
                     PDCAIATDVTIPGDHPGIEKKDASVEMGRGPVVSIVNASGRGLISDPRMVAWLRETAE
                     RKKIPCQVEVGTGGNTDATIIHLERGGIPSVPLSIAARYIHSPVEVVDMGDIEAAIQL
                     LVEALKTKPAL"
     gene            complement(3050..4528)
                     /locus_tag="PK196_07450"
     CDS             complement(3050..4528)
                     /locus_tag="PK196_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HON41288.1"
                     /translation="MFRPSAYVGRVMRIDPVRRQSVISLGSTLALTAIGFISTMFFAH
                     TVGPAILGAYFLFVAYYSVFNLIGDGGFGGAAVKRISEGVDQNAYFTAFIFLRVMLLV
                     ASVGLLLLLRPLLVDLTSSGAFPWLLVALVVSVFTNVAANGVYGTGKVGINQIGNLID
                     TLVRITIQVIAVILGYGIAGLAGGFVAGLIACGLFTFRFLDLSPAPFGLPHLRSLFSF
                     SVWTFLSSSGYLVFSYADTIMIGYFMTDVDVGIYRVALQLTSIATFVTLALHTTLFPK
                     ISYWGKQGELSQVEHAIARGFTYSLLLAVPVVFGGWLLGERLLYFFYGAAFSVGAGAL
                     AILLPVQIAHVFMFLQTMSLNALNRPQDSFRVTAIAVCANILLNLGLIPLYGITGAAM
                     ATLITMALNATLAGRSLSRCIRVRVESRPVANIILAALVMSAAVAAFSYAVPLTNVFV
                     LLGAIALGGLAYMLVLLKLDRGIRDELKDLAEGLGIPWPEML"
BASE COUNT         1004 a         1244 c         1487 g          794 t
ORIGIN      
        1 taactccgga ggtagtatga cagttcaccc ggttaagaag atgatgcaga tgatggcggc
       61 caggatcatg atcatctcag acggcatgtc tgatgaagag atcgatacgt ttatctctga
      121 gatcctgaac gcaaaccgtg tctatactct gggcgccggc cgttcagggc tcgtggcaaa
      181 agcatttgcc atgcgcctga tgcatctcgg gttcagggcg tatgtcgttg gggagacggt
      241 gacccctgcc atgaagccgg gggacgccct catcgtcttc tcggggtctg gcgcgaccag
      301 gacggttgcg gatatcgctg agaccgcaaa gaacatcggc gggaaggtct gcctcatcac
      361 ctcaaagaga gactcacgaa tcgcccgaat cgctgattgc gtcgtcatca tcgagagcca
      421 gcgcgacagg gtggctgacg agtctgcaga gttcgagatc cggcagatga tgggggagca
      481 tagatcgttt gcgccgcttg gcaccatctt cgagacgacc gccatggtct ttgcagatgc
      541 gatcatctcc aggttgatgg agatcatgag ctgccggccg gaggatctcc agtgccggca
      601 cgcaaacatt gagtgaggta agtgtatgaa agaacacagg tatgccgacc gggtgctggg
      661 ggtggagatg tccgggatcc gcaggctctt tgatgccggt gggccggacg caatcaatct
      721 tggcatcggg cagccggact ttgacacgcc cgaccacatc aagatggcag caatcgccgc
      781 cataagagag ggtagaacgg gttacacgcc gaacgccggg atcccggaac tccgggaggc
      841 gatctgcggg aagtttgcac gcgagaacaa tctcacttac ctgccggagc agatcctggt
      901 cacggcagga gggagcgagg cgctccacct ggccatggag gcgcttgtaa acccgggcga
      961 ccgtgtcctg gtccctgacc cgggtttcct ctcctacgct gcccttgcgg tcatcgccgg
     1021 cgggcgcccc gttggtgttc cactcgattc aaaccttcat gtggacgttg agcaggcgaa
     1081 agaactgatg gacggggcga ggctccttgt cctgaactcg ccggcaaacc cgacgggcgc
     1141 cgtcgagagc gaggagtcta tccgcgccct tgtggagtac gcaaacgacc gcggggttac
     1201 cgtcgtctcg gacgaggtct acgaacactt cgtttacgag aagaaacacg tcagcgcggc
     1261 ccgttttggt gacgatgtca tcaccgtcaa cgccgcaagc aagacctacg ccatgaccgg
     1321 ctggaggctc ggctacctgg cagccccctc agaagactac atcccgcagt tgctcaaggt
     1381 gcaccagtat gctcaggcgt gcgcgacatc aatatcgcag tatgccgcgg tcgctgcata
     1441 cacaggcgac cagacgccgg ttgcacggat gcgggaggag taccgtgcgc ggcgcgacct
     1501 gctctacgag gggctcaccg ggctcgggtt tgatttcccg cggcccgagg gtgcgttcta
     1561 tatgtttgtt cctatggggc agaacctgat acagaaggcc attgcacacg gtgtcataat
     1621 cgtgccgggc gatgcctttg gctccagggc gccagattac gcgcgtatca gttacgcgac
     1681 atcgagacag aacctcacga gggcgatcgc gcgtctcggg gagttgatga ggtgagcgaa
     1741 gaatggtaaa agagttgttg cggaagttgt ccgacgccca tggcgtctcg ggctgcgagg
     1801 ggagcattag ggatatcata agggctgagg ttgccgggtc ggtcgacgag atccgggagg
     1861 acagtatggg caacctgatc gctgtaaaac gcggcgatgg gttctcgatc atgcttgccg
     1921 cccacatgga cgagataggg ctgatggtcc agtatatcga tgagaagggg ttcatcaggg
     1981 tcgtcccgat cgggggatgg ttcgggccgg tgctctacac gcagcgggtg atcctgcacg
     2041 gcaaaaacgg cccggtcatg ggcgttctcg gcgcgaaacc gccgcacatg ctgaaagagg
     2101 aggagcgcaa gaaggagatc aagatcgagg atatgtttgt cgatgtgggt gctgcaagcg
     2161 aggaagaggt aaagagcctc gggatcgaga tcggcacccc catcaccatc gaccgcgagt
     2221 ttgcagacct tgccggcaac cgcgtcaccg gaaaagcgct cgacaaccgg gttggtgttg
     2281 cgatgctcat acgggcgctg cagcggatgg agtcgcccca caccgtctac gccgtcttca
     2341 ccgtccagga ggaggttgga ctcaagggtg cgaaggtgag cgcatactcc ctgaatcccg
     2401 actgtgccat cgcaaccgat gtcaccatcc ccggcgatca tcccgggatc gagaagaagg
     2461 atgcaagcgt cgagatgggc agggggcctg tcgtgagcat cgtcaacgcg agcgggcgcg
     2521 ggctcatatc cgatcccagg atggtcgcct ggctgcgtga gacggcggag aggaagaaga
     2581 tcccatgcca ggtcgaggtg gggacaggcg gaaacaccga tgcgacgata atccatcttg
     2641 agcgcggggg tatcccgagc gttccgctat ctattgccgc gcgttacatc cactccccgg
     2701 tcgaggtggt ggacatgggt gacatcgagg cggctatcca actcctggtt gaggcgctga
     2761 agaccaaacc cgcgctctga tattttttgc ccgggtggca cggggaaggg tgacgggggg
     2821 cggagcggga agaccccacc cttcagggtg tggatgtatc gtggagcgcc ttcttcacga
     2881 ttttgatacc tcctcgaagt gtagcaggtg tggagacgca ccgtggaccc gcctcaataa
     2941 agggggagac gggatagttc acggccacag gctgccggaa tactggttta gccactcccc
     3001 ctttgaaagc agcgtggcag agcaggtggt ccttctattt gcttgggtct cagagcatct
     3061 ccggccaggg gatgccaaga ccctctgcca ggtccttcag ctcgtcacgg atgccgcggt
     3121 cgagtttgag gagaaccagc atatacgcaa gcccgccgag tgcgatcgcg cccagaagga
     3181 caaagacgtt tgtgagcggg acggcgtatg aaaacgccgc aacagcagcg ctcatgacaa
     3241 gagcggcaag gatgatgttt gccaccggcc gggactccac ccgcacgcgg atgcaccttg
     3301 agaggctgcg gccggcaagg gtcgcgttca gcgccatggt gatgagcgtc gccatcgccg
     3361 ccccggtgat gccgtataac gggatgaggc ccaggttcag gagaatgttt gcacagaccg
     3421 ctatagccgt cacccggaac gagtcctgcg gccggttgag ggcgttgagg gacatcgtct
     3481 ggaggaacat aaagacatgg gctatctgga cgggcaggag tatggcgaga gcgcccgccc
     3541 cgaccgagaa cgccgccccg tagaagaagt agaggagtcg ttcacccagg agccagccgc
     3601 caaagaccac cggaaccgcg agcaggagag agtatgtgaa ccccctggcg atggcgtgct
     3661 ccacctggga gagttctccc tgtttccccc agtaactgat cttcgggaag agggtggtat
     3721 ggagtgcaag cgtgacaaac gtcgcgatcg aggtcaactg gagcgccaca cggtatatcc
     3781 ccacatcgac gtcggtcatg aagtagccga tcatgatcgt atccgcatac gagaagacaa
     3841 ggtagccgct cgagctgagg aacgtccaga ccgagaagga gaagaggctg cggagatgcg
     3901 gcagcccgaa cggtgcaggc gagaggtcca ggaaccggaa ggtgaagaga ccgcaggcga
     3961 tgagcccggc gacaaacccg ccggcgagac ctgcaatccc gtagcccagg atgaccgcaa
     4021 tgacctggat ggttatcctg accagggtgt cgatcaggtt accgatctgg ttgatgccga
     4081 ccttcccggt tccatatacg ccatttgctg caacattggt aaagacgctg acaaccagtg
     4141 cgacaagaag ccagggaaag gcccctgatg aggtgagatc gacgaggagc gggcgcagca
     4201 ggaggaggag acccaccgag gcaacaagca gcatcacccg caggaagata aacgcagtga
     4261 agtatgcgtt ctggtccaca ccctcgctga tccgtttgac cgcggcaccc ccgaaccccc
     4321 cgtccccgat caggttgaag acgctgtagt aggcgacgaa gaggaagtaa gcaccaagga
     4381 tagcgggacc cacggtatgg gcaaagaaca tagtggagat gaacccgatc gcggtcagcg
     4441 cgagcgttga gccaagactg atcacgctct ggcgcctcac ggggtcgatg cgcatgacgc
     4501 ggccgacgta agcggagggg cgaaacaca
//
LOCUS       DAOSZX010000109         4478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_15917_length_4478_cov_19.182455, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000109 DAOSZX010000000
VERSION     DAOSZX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15917_length_4478_cov_19.182455"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..756
                     /locus_tag="PK196_07455"
     CDS             52..756
                     /locus_tag="PK196_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HON41289.1"
                     /translation="MFGRYRGTFRRESGEILVEVEEDDGLLTYRRRCGEDKFERILVA
                     GMAEVIINPVEPVNLPKGITNFLMIEFPPVMIEPGASRTVYLKFPVEFGVFVESARDI
                     EAVDIFAPTTQKYTLYGTPTRGLIARWYGSEVYTEIPEVDLYREGVMEFTITNATREW
                     VEVSRAVFESVDMKIYYGDFVASTATMRVVGPNIAETDFIDAPPRPGMVKSIELYTAR
                     KISALGRGYLMEWGLS"
     gene            756..>1404
                     /locus_tag="PK196_07460"
     CDS             756..>1404
                     /locus_tag="PK196_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HON41290.1"
                     /translation="MFNMTAVLETPLGVGGITIESLLFFTLILVIGVLIARIVSINVR
                     RALSERLPVNERELLTKLVYYGIVILAFLAALPQLNVDLSGLLVAGGIAGIVIGFASQ
                     SVISNLISGLFLMFERPIQIGDNINVSDVTGSVEDIRVLSTVIKTFDGIYIRIPNEKV
                     FTSNITNYVHNVARRFEYLISIRYQDDADLAIRIAKEVIETHPFALRNPSPSVFVD"
     assembly_gap    1405..1504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1505..2106)
                     /locus_tag="PK196_07465"
     CDS             complement(<1505..2106)
                     /locus_tag="PK196_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON41291.1"
                     /translation="MFTITEVYNNIPYREGLTTDWGFSCLVEEAGLLFDTGAKGDILI
                     TNMQALEIDPAKIRSVVLSHDHHDHVGGLASILDANPRLEVFVHSGFSRETLERISDL
                     TIPQVVRGWVEIADGVAVTGPLGATIQEQSLALETPAGFLIITGCAHPHIARIIERVS
                     QEGRVWGVIGGLHSLSRRDLKELARLTSISASHCTENIAHL"
     gene            complement(2176..>2593)
                     /locus_tag="PK196_07470"
     CDS             complement(2176..>2593)
                     /locus_tag="PK196_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844608.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HON41292.1"
                     /translation="SGLITSKVRYYRVVNTLQVTLNDVGRAGEVVDLAVENGANRVNS
                     LAFTLSDEKQQEYRSQALTAAVAQARGDADAVAAALGKSIVDVKEVNVGGSYVPMAYD
                     NRYVMEQAAGAGVKTTPLEVGEIELTASVYITYTLS"
     assembly_gap    2594..2693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2694..3691)
                     /locus_tag="PK196_07475"
     CDS             complement(<2694..3691)
                     /locus_tag="PK196_07475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HON41293.1"
                     /translation="MRPEAAALLLVSVMLLALIIPPADAAGPPEHANASSRAVEAVKE
                     ALNRGPADAPGQNKDPDAEEERTESAAPMPGIWAAVLVLVLLAGGSAILLRRLKKREP
                     DACRTVVSRPPGFPPELADRYDRAAFIGRGGLGEVFSARRRDDGRVVAVKVPLARDEV
                     TGRAFMNEMRFLEDLNHPNILAVHSVNILPVPFVEMEYLPRTLKDLDKPLPPETAARI
                     AAGIAAGLAFAHERGVVHLDIKPGNILLADDLTPKIADWGMGRMVETGRDTGVTGYTL
                     AYAAPEQIAPGRYGRPDARTDIYQTGAVFYELVTGRPPFADGNLAGLAEAILNDQPL"
     gene            complement(3681..>4478)
                     /locus_tag="PK196_07480"
     CDS             complement(3681..>4478)
                     /locus_tag="PK196_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HON41294.1"
                     /translation="DDDARTLVLDMEPDDLEELSEYLDVLSSSTRLKILKTIERSPKD
                     IRQISAAIETSYENTKKHLDRLMKIGVVRKEAGLSRPTARGVRPVWKYSLVPGGLEAI
                     TRSLGLFANLRLTLTDEVLAERLASVRETVSGEFGGLSSPAVILLGGPEDGKVFPLDH
                     DRIAIGRLDPAAGAPRGQAIVLSEGYAAVTRVSRPHARITRHRDDGWFIEDCGSTGGT
                     FVNGRLLEEGRRHELHDGDMIDLAKGASGATLIFVAPVGRVMVPDET"
BASE COUNT          773 a         1276 c         1332 g          897 t
ORIGIN      
        1 cctttcagcc ttcccgtagt ttaatatttg agtattcgca gatcattgct gatgttcggg
       61 cgctacagag gcacgttcag gcgtgagtcc ggggagattc tggtcgaggt tgaggaggat
      121 gacggtctcc tgacctacag gcgcaggtgc ggcgaggata agttcgagcg gatccttgta
      181 gccgggatgg ccgaggttat cataaacccg gtggagcctg tcaacctacc aaaaggtatc
      241 acaaacttcc tgatgataga gttccccccg gtgatgatcg agcccggtgc ctcccggacg
      301 gtatacctga agtttccagt tgagttcggc gtctttgtgg agtctgcgcg ggatatcgag
      361 gcagtggata tctttgcccc caccacccag aagtacaccc tatacggcac accgacccgc
      421 gggctcatag cacgctggta tggaagcgag gtctacaccg agattccaga ggtcgatctc
      481 taccgcgagg gggtgatgga gtttacaatc acaaacgcca cccgcgagtg ggtggaggtc
      541 tccagggccg tctttgagag cgtggatatg aagatctact acggcgactt tgtggcctcg
      601 accgcgacga tgcgggttgt tggcccgaac attgcggaga ccgattttat agacgcgccg
      661 ccgcggccag gtatggtgaa gtcgattgaa ctttacaccg ctcgaaagat ctcggcgctc
      721 ggccggggct acctgatgga gtgggggctc tcgtaatgtt caacatgacc gcggtcctgg
      781 agacgcccct cggcgtgggc ggtatcacca ttgagagcct cctcttcttc accctgatcc
      841 tggttatcgg cgtgcttatc gcgaggatcg tctcgataaa cgtcaggagg gccctctcgg
      901 aacgattgcc tgtgaacgaa cgcgaactgc tcacgaaact ggtctactac ggtatcgtca
      961 tcttggcgtt tctggccgct ctcccccagc tcaacgttga cctctccggt ctcctggtgg
     1021 ccgggggtat cgccggtata gttatcgggt ttgcaagcca gagcgtcata tcgaacctga
     1081 tctccgggct cttcctgatg tttgagcgcc cgatccagat cggcgataac ataaacgtct
     1141 cggatgtcac agggtctgtg gaggatatca gggtactctc gacggtcata aagacctttg
     1201 atgggatata catccggatc ccgaacgaga aggtcttcac ctcaaatata acaaactacg
     1261 tccataacgt tgcccggcgg tttgagtatc tgatcagcat ccgataccag gacgatgctg
     1321 atctggcgat ccggattgca aaggaggtca tcgagaccca cccgtttgcg ctcaggaacc
     1381 cttcgccctc ggtctttgta gacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnaggtgc gcaatatttt cggtgcagtg cgatgccgag atcgaggtca gcctggcaag
     1561 ctctttgaga tcccggcgag agaggctgtg caacccgccg atgacgcccc ataccctccc
     1621 ctcctgcgag accctctcga tgatccgggc gatgtgagga tgggcgcagc cggttattat
     1681 caggaagccg gcaggcgttt cgagtgcaag cgactgctcc tggattgtcg ccccgagcgg
     1741 gccggtcacg gcaacgccgt ctgcgatctc cacccagcca cggacgacct gcgggattgt
     1801 caggtcgctg atccgctcaa gggtctctct ggagaagccg ctgtggacga agacctccag
     1861 ccgcgggttt gcatcgagga ttgacgcaag cccgccaacg tggtcgtggt ggtcgtgcga
     1921 gagcacaaca gagcgtatct tcgcaggatc gatctcgagc gcctgcatgt tcgtgatgag
     1981 gatatcccct ttcgccccgg tatcaaagag cagccccgcc tcctccacaa ggcaggagaa
     2041 cccccagtcg gttgtgagcc cctcacggta ggggatgttg ttgtagacct cggttatggt
     2101 gaacatgaat caggttcacc tccgcggcgg gattatcctt cccgatgggg cagtggagaa
     2161 aaaagggggt tttgtctaag agagggtgta tgtgatgtag acgcttgcgg tcagttcgat
     2221 ctcgccgacc tcaagcgggg tggtctttac gccggcaccc gctgcctgct ccataacgta
     2281 gcggttgtcg taggccatgg gcacgtagct tcccccgacg ttgacctcct tgacatcaac
     2341 gatactcttt ccaagcgccg cggcgaccgc gtcagcatcg ccccgcgcct gcgccacggc
     2401 ggcggtcagc gcctgggagc ggtactcctg ctgtttttcg tcgctcagtg tgaaggccag
     2461 ggagttaacc ctgtttgctc cgttctcgac cgccaggtcg acaacctcac ccgcgcggcc
     2521 gacgtcgttc aacgtaacct ggagggtgtt gaccacccgg tagtacctga cctttgatgt
     2581 tatcaggccg ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggggct
     2701 gatcattcag gatggcctct gcaagaccgg cgaggttacc gtctgcaaac ggcggtctgc
     2761 cggttaccag ttcgtagaag acggcgcctg tctggtatat gtcggtcctg gcatcgggtc
     2821 ggccgtaacg gccgggggcg atctgttcgg gggcggcgta ggccagggtg tagccggtaa
     2881 ccccggtatc ccggccggtc tcaaccattc tgcccatccc ccagtcggcg atcttagggg
     2941 tgaggtcgtc ggcaagaagg atgttccccg gctttatatc gaggtggaca acccctctct
     3001 catgggcaaa ggcaagtccc gcggctatcc cggcggctat ccgtgcggcg gtctccggcg
     3061 ggaggggttt atcaaggtct ttcaacgtcc gggggaggta ctccatctcg acgaagggca
     3121 ccgggaggat gttgaccgag tggacggcca ggatgttggg gtggttgagg tcttccagga
     3181 accgcatctc gttcatgaac gctctcccgg taacctcgtc gcgggcgagc gggaccttca
     3241 cggccaccac ccggccgtcg tctcgccttc gtgccgagaa gacctctcca agtccgcctc
     3301 tccctatgaa ggcggcccgg tcgtagcggt cagcaagttc tggcgggaac cccggcggcc
     3361 gggagaccac cgtccggcag gcatcgggct ccctcttctt caggcggcgg aggagtatgg
     3421 cagacccgcc ggcgagaaga acgagaacca ggacggctgc ccagatgccc ggcatcggtg
     3481 ccgcgctctc cgttctctct tcctctgcat ccgggtcttt gttctgtccg ggcgcatcgg
     3541 ccggacccct gttcagcgcc tctttgaccg cctcgaccgc ccgggacgag gcgttcgcgt
     3601 gttcaggcgg ccctgctgcg tcggcgggag ggatgatgag ggcaaggagc atgacggaga
     3661 ccaggagcag ggcggccgct tcaggtctca tccggcacca taaccctccc caccggggcg
     3721 acaaagataa gtgtcgcccc cgaagcaccc tttgcaagat cgatcatgtc gccatcgtgg
     3781 agttcgtgtc gcctgccctc ctcaagcagt ctgccgttca cgaaggtgcc gccggtgctg
     3841 ccgcagtcct cgatgaacca gccgtcgtcg cgatgccggg tgatccgggc gtgcggccgc
     3901 gagacgcggg tgaccgctgc atacccctcg ctgaggacaa tcgcctggcc ccgtggggcg
     3961 cctgcggccg ggtcgagccg gccaatggcg atacggtcgt ggtcgagggg gaagaccttg
     4021 ccgtcctccg ggccgccgag gaggatgacg gccggggagg agagtccgcc aaactctccg
     4081 gagacggtct cgcggacact ggccagcctc tctgccagaa cctcgtctgt gagagtcagc
     4141 cgcaggtttg caaagagccc gagactcctt gtgatggcct caagcccgcc gggtacgagc
     4201 gaatacttcc agaccggccg gacacccctg gcggtggggc gtgatagacc ggcctctttt
     4261 cggacgaccc cgatcttcat cagtctgtcc aggtgcttct ttgtgttctc gtaacttgtc
     4321 tcgatcgccg ccgagatctg gcggatgtct tttggactcc gctcgatggt tttcaggatc
     4381 ttcagcctgg tgctgctgct gagaacgtcc aggtattcgg agagctcctc caggtcgtcg
     4441 ggctccatat ccaggacaag cgtccttgcg tcatcgtc
//
LOCUS       DAOSZX010000110         4453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_16017_length_4453_cov_20.622101, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000110 DAOSZX010000000
VERSION     DAOSZX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16017_length_4453_cov_20.622101"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..584
                     /gene="bcp"
                     /locus_tag="PK196_07485"
     CDS             96..584
                     /gene="bcp"
                     /locus_tag="PK196_07485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HON41295.1"
                     /translation="MTELSPGMPAPDFTLPDADGQPVSLQDMRGAYVVVYFYPKDNTS
                     ACTLEARSFTEEMEEFERLNARVIGISPDPPRSHKRFAEKHNLSVHLLSDPDHDVIAA
                     YGAWVRKKRCGREYEGVDRSTFIIDPEGKVAAVWRNVKVDGHVDEVKAKLEELAAASA
                     AS"
     gene            complement(579..1238)
                     /locus_tag="PK196_07490"
     CDS             complement(579..1238)
                     /locus_tag="PK196_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41296.1"
                     /translation="MADDMEIIRSALRRAREDGADAAGALPAANLLDCPSAAADGWRG
                     ARDKRGAYIVIGLYHDPGKPELDLWEPGLGTPGDRILAGIGKDVVRWLQENHGTSARI
                     IPYQIEDGGIYLKDAACLAGIGRMGRNNLVIVPRYGPRVRFRAVWADLDCPEPDQPGA
                     EDPCRDCPGCCISACPMDAFASGRYERERCMARMIADKASGRGRIDHCRACELACREG
                     L"
     gene            1336..3345
                     /locus_tag="PK196_07495"
     CDS             1336..3345
                     /locus_tag="PK196_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HON41297.1"
                     /translation="MQRFGARGDHGGGGVSTTREASTSCSICRKGVCATCGPVLDRRE
                     IAVFILAGLLLLFGMLAVYATTWTLAGNLLLLAAAVVSGRDVLKTAAVSLIRFRFSIA
                     VLIAIAAAGAFLIGNPAEGATVLYLYAIAEFLEEYAAGRAERSIASLLHLAPQTARVR
                     RDGAEVTVGVEEVGVGETVIVRPGDIIPLDGVVVAGGSSVDQAAITGESVPVAKGAGD
                     DVYAGTQCIEGYLEVRVTKPVKESTIARVAALVEEAQAHPSTMETFIERFSRYYTPGV
                     ILAATVLMIVPPVAFGVPFLEAIYRALVLLVIACPCALAISTPVSMVSGITTAAHNGV
                     LIKGRDALEAVGRARVMVFDKTGTLTTGRLEVEDVIGFGVPEADVLAIAASLESRSGH
                     PIAEAIRRRAGRECIVLDEVEEFVSTAGRGVRGRIGGSVYTVGNSAPSSGEWRSVYER
                     LEAEGKTVVLVGTVSGVIGLIALSDVIKEDAEATVAALRARRIRTVMLTGDNERVGMA
                     VARRIGIDECYAGLLPEEKVAMVERLMERYGIVVMVGDGVNDAPALARANVGVAMGAI
                     GSDAAIEAADVVVMEDEISRVAYLVALSEKTVSVVRQNVTSAVVVKLGIAALAVPGLV
                     SLWMAVAVGDMGLSLAVIANALRVGRPGGAGRQASGDWPDGRARR"
     gene            3627..4448
                     /locus_tag="PK196_07500"
     CDS             3627..4448
                     /locus_tag="PK196_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41298.1"
                     /translation="MKKNAATQISMLLIAVALLAPAVQAALVVDAGDDLSVLAGEPVT
                     VLAACNDTDGIDLLNLSAFIDWGTVRTPAEILADDEQNATIQATHRYPVAGIYTVTVA
                     VENNATGNVTTDSLNVTVNPQTAAVGVAPGVLNQKSRGLMTVFVSVSEWLGFAEADEA
                     NVTIADPSIFTIGNATPAKVNYCMKDGGTFILKYRRADVAPLAAEGNLTVNGSAVTED
                     GNVTVIGRGAVSLIEPGNGKGPSPGNPEGHPLAERVKERTKNENAFRDRERSSGT"
BASE COUNT          814 a         1312 c         1475 g          852 t
ORIGIN      
        1 tcgagggaga acccttcata ccaactgcgg cttacaggta tacctgacct gttaccctgg
       61 atattcttat atcctcaagg gtatctctca ctgctatgac agaactctca cccggcatgc
      121 cggcgcccga cttcaccctc ccggatgctg acggccagcc cgtatcgctc caggatatgc
      181 ggggtgccta tgtggtggtc tacttctacc cgaaggacaa cacatcggcc tgcaccctgg
      241 aagccaggtc gttcacagag gagatggagg agtttgaacg cctgaacgcc agggttatag
      301 gtatcagccc ggacccgccg cggagccaca aacggtttgc cgagaagcac aacctctccg
      361 ttcatctcct atccgacccc gaccacgacg tgatcgcggc atacggcgcc tgggtcagga
      421 agaagcgttg cggcagggag tatgagggtg tcgaccggag caccttcatc atcgatcccg
      481 agggaaaggt cgctgcagta tggaggaacg tgaaggtgga tgggcacgtg gatgaggtga
      541 aggcaaaact tgaggaactg gcggcagcgt ccgccgcctc atagcccttc cctgcaggcg
      601 agttcgcacg cccggcagtg atcgatccgc cccctccctg aggctttgtc ggcgatcatc
      661 cgcgccatgc accgctcccg ctcataccgg ccggaagcga aggcatccat cggacaggcg
      721 ctgatgcagc agcccgggca gtctctgcaa gggtcttcgg ccccgggctg atccggttcc
      781 gggcagtcca ggtcggccca taccgcccgg aaccgtaccc tggggccgta acgggggacg
      841 atgacaaggt tgttcctgcc catccgcccg attccggcga ggcaggccgc gtccttgagg
      901 tagatcccgc catcctctat ctggtagggg atgatacgtg cgctggtgcc gtggttctcc
      961 tgcagccagc ggacaacgtc cttcccgatc cccgccagga tccgatcccc gggcgtcccc
     1021 agaccgggtt cccagaggtc gagttcgggt tttccggggt cgtggtagag gccaatgacg
     1081 atataggccc cccgtttgtc ccgcgctccc cgccatccat ccgcggccgc cgaggggcag
     1141 tcgaggaggt ttgcggccgg cagagcgccg gccgcgtccg ccccgtcttc acgtgccctc
     1201 cggagcgccg atcttataat ctccatgtcg tctgccatcc ctggagggtt atggtgcggc
     1261 cggtaaaaaa cagacctttt ggggcacacc cttcaaatca tattttacgg ctgtgataca
     1321 gaagataggg taaccatgca gcggttcggt gcgagagggg atcatggcgg aggtggggtc
     1381 tccacgacac gagaggcatc aacgtcctgc agcatctgcc gcaagggggt ctgcgcgacc
     1441 tgcggcccgg ttctcgaccg ccgggagatc gcggtcttca tcctcgcggg tctcctcctc
     1501 ctcttcggga tgcttgccgt ctatgccacc acatggacgc ttgccggaaa cctgctgctg
     1561 ctcgctgcag cggtcgtatc cggccgcgat gtcctcaaga ccgcggcggt ctctctcatc
     1621 cggttccggt tcagcatcgc cgtgctcatc gccatcgccg cagcgggcgc gtttttgata
     1681 gggaatcctg ccgaaggggc gaccgtgctc tacctctacg ccatcgccga gttcctggag
     1741 gagtacgccg ccgggagggc tgagcgctcg atagcctcgc tcctccacct ggcgccacag
     1801 acggccaggg tgaggcggga cggtgccgag gtgacggtcg gcgttgagga ggttggtgtt
     1861 ggcgagaccg tgatcgtgag accgggggat atcatcccgc tcgacggggt cgtcgttgcc
     1921 ggaggctcgt cggtcgacca ggcggctatc accggggaga gcgtcccggt ggcgaagggc
     1981 gcgggggacg atgtatacgc tggaacgcag tgcatcgagg gctaccttga ggtcagggtg
     2041 acgaaaccgg tgaaggagag caccatagcc cgtgttgcgg cgctggtcga ggaggcgcag
     2101 gcccacccct ccaccatgga aaccttcatc gagcggttct cccgctacta cacgccgggt
     2161 gtgatactcg ccgctacggt cctgatgatc gttcctccgg ttgctttcgg cgtgccgttc
     2221 cttgaggcga tctacagggc gctcgtcctg ctggtcatcg cctgcccctg cgcgcttgcc
     2281 atatccaccc cggtctcgat ggtatcgggg atcacgacgg cggcgcataa cggtgtgctg
     2341 atcaagggtc gtgacgccct ggaggctgtg ggtcgcgcaa gggtgatggt ctttgacaag
     2401 acagggacgc tcacaaccgg gaggcttgag gtggaggatg ttatagggtt tggcgtcccg
     2461 gaggctgatg tgcttgccat agccgcgtcg cttgaatccc gatccggcca cccgatcgcg
     2521 gaggcgatca ggcggcgtgc cgggagggag tgtatcgttc tggatgaagt cgaggagttc
     2581 gtctcgaccg cggggagggg tgttaggggg aggatcggcg gcagcgtcta cacggttgga
     2641 aactctgcac catccagcgg tgaatggcga tcggtctatg aacggctgga ggctgaggga
     2701 aagaccgtcg ttctcgtcgg gacagtctca ggggttatcg ggcttatagc gctctcggac
     2761 gtcatcaagg aggatgcaga ggcaacggtt gccgctctca gggcgcggcg gatccggacg
     2821 gtgatgctca cgggggataa cgagcgcgtc ggcatggcgg tggcacggcg catcgggatc
     2881 gatgagtgtt atgcgggtct cctccccgag gagaaggtgg cgatggtcga gcggctgatg
     2941 gagcgctacg gcatcgtggt catggtcggc gacggcgtga acgacgcccc cgcacttgcc
     3001 cgggcaaacg tcggcgtggc gatgggggcg ataggctcgg atgctgcgat agaggctgcg
     3061 gatgtcgttg tgatggagga tgagatctca cgtgttgcct accttgtggc gctctcggag
     3121 aagacggtct ctgtcgtccg gcagaacgtc acatcggcgg tcgtcgttaa actcgggatc
     3181 gccgcgctcg ccgtacccgg tctggtctca ctctggatgg cggtggcggt cggggatatg
     3241 gggctctcgc ttgcggtcat agcaaacgct ctccgggtag gccggccggg tggtgcaggc
     3301 aggcaggcct ccggcgattg gcccgatggc cgtgcccgga gatgagattt cgggaatcgg
     3361 tttcacagaa tcgtcagaga gaggataacc tccagggcac cctatcgatg gggaatcgcc
     3421 cgtgagggac ggcacgtccc ccgcccctct ctcctgcatg ccaccggcaa acgcccttta
     3481 tttcagagca ccccaacctc ccatttatgt ggcgttgaag aacccatctc gtctttctgg
     3541 cagcagctgg ttcactgatc taacccggga gctcaatgcg ctcaaatagg ggatggagga
     3601 gagtacctgg ctgaggtgat gtatccatga agaagaatgc tgccactcag atcagcatgc
     3661 tgctgatcgc tgttgccctg ctggcccctg cggtgcaggc cgctctggtg gtcgatgcag
     3721 gtgatgacct cagcgtgctt gccggggaac cggtaaccgt tctcgcggcc tgcaacgaca
     3781 cggacggcat cgatctgctg aacctctctg ccttcatcga ctggggcaca gtgagaacgc
     3841 cagcagagat tctggcggac gatgagcaga acgcaaccat ccaggcaacc cacagatacc
     3901 cggttgcagg catctacacg gtcactgtgg cggttgagaa caacgctacc ggcaacgtga
     3961 ccaccgattc cctgaacgtg acggtgaacc cgcagacagc ggcggtcggg gtggctcctg
     4021 gtgttctgaa ccagaagagc aggggtctca tgaccgtctt tgtcagtgtt tccgaatggc
     4081 tcgggtttgc cgaagccgat gaggcaaacg tgacgatagc ggatccctcg atctttacaa
     4141 tcggaaacgc aacccccgcg aaggtcaact actgcatgaa ggatggcggc acgttcatcc
     4201 tgaagtatcg gcgggcggac gttgctccgc tggcggctga gggtaatctg accgttaacg
     4261 gcagtgcagt gacagaggac gggaatgtca ctgtcatcgg cagaggtgcc gtctctctca
     4321 tcgagcccgg caacgggaag gggccgtctc ccggcaaccc tgaggggcat cccctggcag
     4381 agagggtgaa ggagagaacg aagaacgaga acgcattccg tgaccgcgaa cggagttccg
     4441 gcacctgatc aaa
//
LOCUS       DAOSZX010000111         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_16236_length_4406_cov_21.043668, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000111 DAOSZX010000000
VERSION     DAOSZX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_4406_cov_21.043668"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..698
                     /locus_tag="PK196_07505"
     CDS             108..698
                     /locus_tag="PK196_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HON41299.1"
                     /translation="MAKSMYAYVREAWKQPDTSEVKDLLWERLQVWRREGSVVRVERP
                     TRIDRARSLGYKAKQGIIVVRVKIRRGGRRKPRYVRGRRTARMGMHRMTPAKSLQRMA
                     EERASRRYPNMEALNSYWVGEDGRHKWFEVILVDGHHPAIQSDRNLSWLADPVHRGRA
                     ERGKTSAGVKGRGMRKRGSGTEKTRPSIRSHANRGK"
     gene            718..1365
                     /locus_tag="PK196_07510"
     CDS             718..1365
                     /locus_tag="PK196_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HON41300.1"
                     /translation="MKITDACIFPYPDGDSTVSRMAIEAGELGFDSLVVIGDAGHRLS
                     SGVEILQGAIITARSIKEVLRDLRDPAVRRADLVCVDAGDLSFNRALASTKEVNVISK
                     IHATRQNAFDHVAARTAAERGVAVEISIAPLIHLRGMKRQQALNRYADILALHRRYGF
                     MLTLSSAASSILDQRSVREIRGLCGLFGMTGAEVNEALSSVGRMIEPSGPVGAVR"
     gene            1362..1844
                     /locus_tag="PK196_07515"
     CDS             1362..1844
                     /locus_tag="PK196_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HON41301.1"
                     /translation="MRPRPPTMRTKRRYILARILPYGAAVDQKQLYLSVIEAAASLLG
                     DAAVGLAQPAVVYCDAGYAVIRCRRGAERDIAAALATVTAVGDDRVSLRTVAASGTIR
                     ALRRRMRPIRPIPEDEEITIGERAYAVCRYPGQKVDLIEKGIKHQKRFFFSELDLEER
                     "
     gene            1844..2581
                     /gene="psmA"
                     /locus_tag="PK196_07520"
     CDS             1844..2581
                     /gene="psmA"
                     /locus_tag="PK196_07520"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HON41302.1"
                     /translation="MQPQYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGLKCS
                     EGVVLIVDKRVTSRLLEAASIEKIFKIDAHIGVASSGLVGDARSLVDRARVEAQINRV
                     SYNEPIDVETLAKKLCDHMQIYTQFGGARPYGTALLIAGISDGKGRLFETDPSGTLLE
                     YRATGIGTGRPAVMQVFEEEYREDATFSEAIALGIKALHAATEGKLDVSAIEIGVVSI
                     ETGGFRKMERDEIQSYVDKFEESVPSA"
     gene            2578..3282
                     /locus_tag="PK196_07525"
     CDS             2578..3282
                     /locus_tag="PK196_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HON41303.1"
                     /translation="MIPLDRAVVARFESHGERFEVLVDPDQAMRIRQGEAIDLEDVVA
                     ADSVFSNAAHGERASDEALMKVFKTTEFEEIAPKIIQKGEIHLTAEQRRHIVADKRNR
                     VITFIARNAINPQSGYPHPPQRIELAMEEAKVNIDPFKSVEEQVKEVVKALRPILPIR
                     FEEIRIAVRFPPEHAARAYGELQAAAAIEQNEWQKDGSWIAVVRIPAGIQEEFYNLVN
                     KISRGSAETRIVGRVS"
     gene            3339..3629
                     /locus_tag="PK196_07530"
     CDS             3339..3629
                     /locus_tag="PK196_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HON41304.1"
                     /translation="MAGRKQSAKGRVVGSSGRYGPRYGRFIRKRVNQIEQISRAAHVC
                     PRCDREAVRRVGTGIWECRKCGFRFAGGSYVPETPVMRVAARSIERSLQKEG"
     gene            3636..3767
                     /locus_tag="PK196_07535"
     CDS             3636..3767
                     /locus_tag="PK196_07535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HON41305.1"
                     /translation="MVAYKCARCKQKVEIDINIRCPYCGHRILFKERGAAIKDLKAR"
     gene            3764..4174
                     /locus_tag="PK196_07540"
     CDS             3764..4174
                     /locus_tag="PK196_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41306.1"
                     /translation="MTVVTTSRKPVPEVRSLARDLAFAIGAEYATRGKMGMGDLLSSS
                     HDGLILIVSRSGQSFLIQVFSGGEAVAGIRVLSFAVEEREGAISRGLAVSDRALASAL
                     KGLADMVFFVDGLPEDRIVFDGRQRRRYTLRVAP"
     gene            4171..>4406
                     /locus_tag="PK196_07545"
     CDS             4171..>4406
                     /locus_tag="PK196_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HON41307.1"
                     /translation="MRHSAVFRFTAPDARAIYLSVCQEEGDVGERSSVRVRLEDEETL
                     ILEVSAADIPALRAALNTWLRLINIAVEVREAALS"
BASE COUNT          879 a         1228 c         1489 g          810 t
ORIGIN      
        1 gggagacgga atcctttatc agcgcccccg tcgatctata tggagccaac gggatacgtc
       61 ccggaaagga atgtggcgga aacgctgaac ctgaggtgat tgagagcatg gcaaaatcga
      121 tgtatgccta cgtacgtgag gcctggaaac agcctgacac gtccgaggtg aaagacctcc
      181 tctgggagcg tctccaggtc tggcggcgcg aggggagtgt cgtgcgcgtt gagcgcccca
      241 caaggatcga ccgcgcccgt tcgcttggct acaaggccaa gcagggcata atcgtcgtgc
      301 gggtcaagat ccgccgcggc ggccggagga agccccgtta cgttcgcggc cgcagaacag
      361 cgcgcatggg tatgcaccgg atgaccccgg caaagagcct ccagcgcatg gccgaggagc
      421 gcgcatcccg caggtacccg aacatggagg ccctgaactc ctactgggtc ggcgaggacg
      481 ggcgccacaa gtggtttgag gtgatcctgg tcgacggcca ccaccctgcc atccagagcg
      541 accggaacct ctcctggctg gccgaccccg ttcaccgggg ccgggcggag cgcggcaaga
      601 cctccgccgg cgtgaagggg cgcgggatgc ggaagcgcgg gagcggaacc gagaagactc
      661 ggcccagcat ccggtcgcat gcaaaccgcg gcaaataaac acttttttta aacgacgatg
      721 aagatcacag atgcctgtat attcccctat cctgacggcg actccacggt ttcgcggatg
      781 gcgatcgagg cgggagaact cgggttcgat agcctcgtcg tcatagggga tgccgggcat
      841 cggttgtcca gtggggttga gatcctccag ggcgctatca tcaccgcccg gtccataaaa
      901 gaggttctgc gcgacctgcg cgaccccgcc gtccggcgcg ccgatctggt ctgcgtcgat
      961 gccggagatc tctcgttcaa ccgcgctctt gcaagcacca aggaggtgaa cgtcatcagt
     1021 aagatccatg ccacccggca gaacgccttt gaccacgtcg ccgcacggac ggcggcggag
     1081 aggggtgtgg cggtggagat ctctattgcc cccctgatcc acctccgcgg gatgaaacgc
     1141 cagcaggcgc tgaaccggta tgcggatatc ctcgcgctgc accggcgtta cgggtttatg
     1201 ctcaccctat cgagcgccgc cagttcgata ctcgaccagc gttcggttcg agagatccgg
     1261 gggctctgcg ggctcttcgg catgaccggg gccgaggtga acgaggctct atcgtctgtg
     1321 ggccggatga tcgagccctc cggccccgtg ggggcggtca gatgagaccc aggcccccga
     1381 cgatgcggac gaaacggcgc tacatactcg caaggatcct tccatacggt gcggcagttg
     1441 accagaaaca actctacctc tccgtcatcg aggccgcggc atccctcctc ggcgatgcgg
     1501 ccgtcggtct cgcccagccc gccgtcgtct actgcgatgc ggggtatgcc gtcatccggt
     1561 gccggcgcgg ggcggaaagg gacatcgccg cagcgcttgc aaccgtcacc gcggtcggcg
     1621 atgaccgggt ttcgctccgg acggtcgccg catccgggac gatccgtgcg ctgcggcgcc
     1681 ggatgagacc tatccggccc attccggagg acgaggagat tacgatcggg gagagggctt
     1741 atgcggtctg ccggtatccg ggtcaaaagg ttgatttgat tgaaaagggt attaagcatc
     1801 agaaaagatt ctttttcagc gaactcgatt tggaggaacg ataatgcagc cacaatatca
     1861 gatgggatac gaccgggcta tcacggtctt cagccccgat ggaagactct atcaggttga
     1921 gtatgcaagg gaagcggtaa aacgcggcac gacggccgtt gggctcaagt gcagcgaggg
     1981 tgtcgtgctg atagtcgaca aaagggtgac gtcccgcctc ctcgaagcgg cctccatcga
     2041 gaagatcttc aagatcgatg cgcacatagg tgtggcctcc tccggtcttg tgggggatgc
     2101 acgctccctc gtggatcggg cgcgggtcga ggcccagatc aacagggtct cctacaacga
     2161 gcctatcgat gtcgagaccc tggccaagaa actctgcgac catatgcaga tctataccca
     2221 gttcggcggt gcgcgtccct acggcacggc gctcctgatc gccgggatca gcgacggtaa
     2281 gggcagactc tttgagaccg acccgagcgg cacgctgctc gagtacaggg caaccgggat
     2341 cgggacaggc cggccggcgg tcatgcaggt ctttgaggag gagtatcgtg aggatgcgac
     2401 cttctcggag gcgatcgctc tcggcatcaa ggcgctccat gcagcgaccg agggaaaact
     2461 ggacgtgagc gcaatcgaga tcggtgttgt ctccatcgag acgggggggt tccggaagat
     2521 ggagagggat gagatccagt cctacgttga taagtttgag gagagcgtgc catcagcatg
     2581 atcccgctcg accgtgcggt tgtggcgagg ttcgagagtc acggggaacg gtttgaggtg
     2641 ctggtcgacc ccgaccaggc gatgcgcatc cggcaggggg aagcgataga ccttgaggac
     2701 gtcgttgcgg ccgactccgt cttctcgaac gccgctcatg gtgagagggc ctcggacgag
     2761 gctctcatga aggtcttcaa gacgaccgag tttgaagaga ttgcgccgaa gatcatccag
     2821 aagggcgaga tccatctgac ggcagagcag cggcgccata tcgtcgcgga caaacggaac
     2881 cgggtcataa cgttcatagc aaggaacgct atcaacccgc agtcgggcta cccacacccc
     2941 ccgcagcgga tcgagctcgc gatggaggag gcgaaggtga acatcgatcc cttcaagtcg
     3001 gtcgaggagc aggtgaagga ggttgtcaag gccctccgcc cgatccttcc catcaggttt
     3061 gaggagatcc ggatcgccgt caggttcccg cctgaacatg ctgcaagggc ttacggggaa
     3121 ctccaggccg cagcagcgat cgagcagaac gagtggcaga aggacggctc ctggatcgcg
     3181 gtggtcagga ttccggccgg tatccaggag gagttctaca acctggtgaa caagatctcg
     3241 agagggagcg ccgagaccag gatcgtcggg cgtgtatcgt gagtaactaa tattagttga
     3301 gaccacagaa gttagaggac atacaagagg taggcgaaat ggcaggccgc aaacagagtg
     3361 caaaaggcag ggttgtcggg agttccggtc gatacggccc caggtacggc aggtttatca
     3421 ggaagagagt gaaccagatc gagcagatct cccgcgcggc tcatgtctgc ccccggtgtg
     3481 accgggaagc ggtcaggcgt gtggggacgg ggatctggga gtgccgcaag tgcgggttca
     3541 ggttcgcagg cgggagttac gtcccggaga cgccggttat gcgtgtcgcc gcgcggagca
     3601 tcgagcggtc gctgcagaag gagggttaac cggccgtggt tgcgtataag tgcgctcggt
     3661 gtaagcagaa ggttgagata gatatcaata tacgctgtcc ctactgtggg caccgcatcc
     3721 tcttcaagga gcgcggggcc gcgataaaag acctgaaggc tcgatgacgg tcgtcacgac
     3781 gtcgcgcaaa ccggttccgg aggtgcggtc actcgcacgg gatcttgcgt ttgcgatcgg
     3841 cgccgaatac gccacccgcg ggaagatggg catgggcgac ctcctgtcgt cgtcccacga
     3901 tgggctgatt ctgatcgtct cgaggtcagg tcaatctttt cttattcagg tcttttcagg
     3961 gggtgaggcg gttgccggga tccgtgttct ctcgtttgcg gtggaggagc gggagggtgc
     4021 gatctcccgc ggtcttgcgg tatctgaccg cgcccttgcc agtgcgctca agggtctggc
     4081 tgatatggtc ttttttgtgg acggtctccc ggaagaccgg atcgtgtttg acgggcggca
     4141 gcggcggcgc tatacgctga gggtggcgcc atgaggcatt cggcggtatt ccggtttacg
     4201 gctcctgatg cccgtgcaat ctacctctcc gtctgccagg aggaggggga tgtgggtgag
     4261 cgttcgtctg tgagggtcag gcttgaggac gaggagaccc ttatcctgga ggtgagcgct
     4321 gcggatatac cggcgcttcg tgcggccctt aacacctggc ttcggctgat caatatagcg
     4381 gttgaggtgc gggaggctgc tctctc
//
LOCUS       DAOSZX010000112         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_16287_length_4394_cov_21.159023, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000112 DAOSZX010000000
VERSION     DAOSZX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16287_length_4394_cov_21.159023"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..815
                     /locus_tag="PK196_07550"
     CDS             324..815
                     /locus_tag="PK196_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HON41308.1"
                     /translation="MESGTVVGSRVFIAENATVIGDVTLADDVSVWFGAVIRGDRDRI
                     TVGAGSNIQDNATVHTTPGHPVSIGSQVSVGHGAILHGCTIRDRVLVGMGAIVLNGAV
                     VGEGSIIGAGAVVTEGKEIPAASLVLGVPGKVVRGTTAEEEAGIVQNADTYVRLAGGY
                     RRG"
     gene            808..2082
                     /locus_tag="PK196_07555"
     CDS             808..2082
                     /locus_tag="PK196_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HON41309.1"
                     /translation="MADAVVIGAGVAGIQAALDLAGHNIHVHLIEREPSIGGHMAQLD
                     KTFPTNDCSMCILSPKMVDVARNPNITIHTCSEVESVEGEVGNFRVRVRKHPRYILEN
                     ECNGCGDCTLICPVEVYNRFDAGVGVRKAIYKPHTQAVPDIVVKDPEHCIECGLCYDV
                     CGRDAILHEDEERTIDLEAASIIVATGYATFDARNKTQLRYLLIPDVITSLEFERLIN
                     ASGPTGGKIRRLSNGRTPRSVAFVQCVGSRDLSVDRPYCSGVCCMYAMKNAMLIREKS
                     PEIEVTVFYNDIRAYGKGYEEYFERAKSLGVRFVRGFPGEVLEENDRLTLIAENTETG
                     EVETVHPDLVVLSVGLEPAGGAEEIARMLGIPRDESGFFGVADQKLGPVLTVKPGIYV
                     AGTATAPRDIPDSVAMGGAAAMRAYLDVIRAG"
     gene            2085..3131
                     /gene="mtnA"
                     /locus_tag="PK196_07560"
     CDS             2085..3131
                     /gene="mtnA"
                     /locus_tag="PK196_07560"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HON41310.1"
                     /translation="MKGETPYTIAWDAENNCIVYIDQTLLPGRYEVARCRTVDELAGA
                     IRRLAIRGAPALGIAGAMGVALAAVRSGERDPVRFRQDLHRAAEILRSTRPTAVNLGW
                     GIDRVLGAADVAGSVEAAKEAAVREASAVAGEDEVTCRRIGAFGAELLPERCTVLTHC
                     NAGALACRCWGTALGVVRSAVEAGKDVRVIACETRPLNQGSRLTCWELARDGIDVTLI
                     TDSAAAYLMRKGVIDAVIVGADRITRDAVFNKIGTYMHAISARHHGIPFYVAAPVSTF
                     DPARSEVDVVVEERDRGELARCGDRMLAPDGASVLNYAFDATPLDLVDAIVTEVGVLR
                     PPYAESFRLIEGKR"
     gene            3128..3832
                     /locus_tag="PK196_07565"
     CDS             3128..3832
                     /locus_tag="PK196_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HON41311.1"
                     /translation="MSLLATGAWGQVMTLIGEVTFFVILGMLLFAVALAFITVASIRR
                     GKFYLPQILIPGMVALEGLVKAFCKLLGLDDRDLITFFITLRNSMNQKSFSEIPVEER
                     AIFLPQCLRSAQCPANLTPEGLKCRNCGRCQVGENILWLEKLGYRVFIVPGSTFIKRM
                     ARKYRPRAIIGVGCLVEVKDGIDMADRMGITAIGVVNLKDGCVETIANWAALRDAALL
                     GLDAASGGVDLHGPAQ"
     gene            complement(3797..4090)
                     /locus_tag="PK196_07570"
     CDS             complement(3797..4090)
                     /locus_tag="PK196_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41312.1"
                     /translation="MVVSGRLELGPGSTVGGFVEAESAIIGHDARIKGPLRVIERATI
                     CDNACLHSIQAGGDVILRPGVKAGVVTSEETIYVYGKVSTEQLLGRAVKVHAP"
BASE COUNT          807 a         1303 c         1481 g          803 t
ORIGIN      
        1 caccacccgg ccgctccgcg gcctcctccc gccccctccc gagggggcgg gcagtctgtg
       61 gcgatagcac cggataccgt gcatggtgag caggaacagg atttcagtca agggtgttca
      121 catcgagccg cggatctgct catcctggcc gggacactgt atcgatggga tatcgccccg
      181 cgggggaggg gctgacgggg agggggacgg aatatccccc gcccctgtcg ctgcgtgtca
      241 ctgaaaacct atctcgctgg aataaccccc gccgccggcg atggagaagg ttttgtatcg
      301 ccagaacaaa tactagactt actatggaga gcggaacggt tgtggggagc cgcgtcttta
      361 tcgcagagaa tgcaacggtc atcggggacg tgacgctcgc cgacgacgtt agtgtctggt
      421 tcggcgctgt tatccgcggt gaccgggaca ggatcacggt tggagcgggc tcaaacatcc
      481 aggacaacgc caccgtccac accacccccg gccacccggt cagcatcggg agccaggtct
      541 ctgtcggcca cggcgccatc ctgcacggct gcaccatccg cgaccgtgtg ctcgtcggga
      601 tgggcgcaat cgtcctgaac ggtgctgtgg tgggggaggg atccatcatc ggcgccggcg
      661 cggtggtgac cgaaggaaaa gagatcccgg ccgcctccct cgttctcggc gtgcccggaa
      721 aggttgttcg ggggacaacc gccgaagagg aggccgggat cgttcagaac gcggacacat
      781 acgtccggct tgcaggaggt taccgccgtg gctgatgccg tcgtcatcgg cgccggggtt
      841 gcgggtatcc aggcagcgct cgacctcgca ggccacaaca tccatgtcca cctcatagaa
      901 cgtgaaccca gcatcggcgg gcacatggca cagctcgata agacgttccc cacaaacgac
      961 tgctccatgt gcatcctctc tccaaagatg gtggacgttg cccggaaccc gaatatcacc
     1021 atccacacct gttccgaggt cgagtcggtc gagggcgagg tcgggaactt cagggtcagg
     1081 gtcaggaagc accctcgcta catcctggag aacgagtgca acggctgcgg ggactgcacc
     1141 ctgatctgtc ctgtggaggt ctacaaccgg tttgacgccg gtgtcggtgt tcgaaaagcc
     1201 atctacaaac cgcataccca ggccgtcccc gatatcgtcg tgaaggaccc tgagcactgc
     1261 atcgagtgcg gtctctgtta cgacgtctgc ggcagggacg caatcctcca cgaggacgag
     1321 gagcgcacga tcgaccttga ggcggcaagc atcatcgttg caaccggtta cgcgacgttt
     1381 gatgccagaa acaagaccca gctgcgctac ctcctgatcc cggacgtcat cacgagcctt
     1441 gagttcgagc ggctgatcaa cgcaagcggc cccacaggcg ggaagatccg gcggctctca
     1501 aacggcagga ctcctagaag cgtggcgttt gtccagtgcg tcggttcacg cgacctctcc
     1561 gtcgaccgcc cctactgttc gggcgtctgc tgcatgtatg ccatgaagaa cgcgatgctc
     1621 atccgggaga agagcccgga gatcgaggtt actgtcttct acaacgatat cagggcatac
     1681 ggcaagggct acgaggagta cttcgagcgg gcaaagagtc ttggcgtcag gtttgtccgg
     1741 gggttcccgg gggaggtgct cgaggaaaac gatcggctga cgctgatcgc ggagaacacc
     1801 gagaccggtg aggtggagac cgtccacccc gaccttgtgg tgctctccgt cgggcttgag
     1861 cccgccggcg gtgcggagga gatcgcccgg atgctcggga tcccgcgtga cgagtcgggg
     1921 ttcttcgggg ttgccgacca gaagctcggc cccgtgctca cggtgaagcc cgggatctat
     1981 gttgcgggaa ccgccaccgc tccgagggac atccccgact cggtcgcgat gggcggggcg
     2041 gcggcgatgc gggcctacct ggacgtgatc cgcgcggggt gaagatgaaa ggagagaccc
     2101 cctacaccat cgcctgggat gcggagaaca actgcatcgt ctacatcgac cagacccttc
     2161 tcccagggcg gtatgaggtt gcgcgctgca gaaccgtcga cgaacttgcc ggtgcgatcc
     2221 ggaggcttgc gatccgcggc gccccggcgc tcgggatcgc aggggcgatg ggtgtggcgc
     2281 ttgcggccgt ccggagcggc gagagggatc ctgtgcggtt ccgccaggat ctccaccggg
     2341 cagccgagat tctccggagc acccggccta ccgcggtcaa cctgggatgg gggatcgacc
     2401 gcgtcctcgg cgcggccgat gtggcagggt cggtcgaggc ggcgaaggag gccgcggtcc
     2461 gcgaggccag cgccgttgcc ggggaggacg aggtgacctg ccggaggatc ggcgcgttcg
     2521 gagccgaact cctgccggag aggtgcacgg tgctcaccca ctgcaacgcg ggggcgcttg
     2581 cctgccgctg ctgggggacg gcgctcgggg tcgtccggtc ggcggttgag gcgggaaagg
     2641 atgtgcgggt gatcgcctgc gagacccggc cgctgaacca gggttcacgc ctgacctgct
     2701 gggaactcgc acgggacggg atcgatgtga ccctcatcac cgactcggca gcggcctatc
     2761 ttatgcggaa aggggtgatc gacgccgtca tcgtcggtgc agacaggatc acgcgggacg
     2821 cggtcttcaa caagatcggg acatacatgc acgccatctc cgcccggcac cacggtatcc
     2881 cgttctatgt ggcggcaccg gtctcgacgt tcgatcctgc gaggagcgag gtggatgtcg
     2941 ttgtcgagga gcgggaccgc ggcgaactcg cccgctgcgg tgaccggatg ctcgcgcccg
     3001 atggtgcgtc ggtcttaaac tacgccttcg acgccactcc gctcgacctg gtcgatgcta
     3061 tcgtcactga ggtcggggtg ctgcggccgc cctatgcgga gtcgttccgg ctgatagagg
     3121 ggaaacgatg agtctccttg caaccggcgc ctgggggcag gtgatgaccc tgattggaga
     3181 ggtgacgttc tttgtaatcc tggggatgct gctcttcgcg gtggcgctcg cattcatcac
     3241 cgttgcttcg atccggaggg ggaagtttta cctgccgcag atcctgatac cgggcatggt
     3301 cgccctggaa gggcttgtga aggcgttctg caaacttctc gggctggatg acagggatct
     3361 catcacgttc ttcataacgc ttcgcaacag tatgaaccag aagtccttct cggagatccc
     3421 ggtggaggag cgtgcgatct tcctgccgca gtgcctgcgt tcggcacagt gtccggcgaa
     3481 cctgacgccg gaaggcctga agtgccggaa ctgcggccgc tgccaggtgg gggagaacat
     3541 tctgtggctt gaaaaactgg gctacagggt attcattgtg ccgggctcga cgttcataaa
     3601 gaggatggcc aggaagtatc gtccgcgggc gatcatcggt gttggctgtc ttgtggaggt
     3661 aaaggacggg atagatatgg ccgaccggat ggggatcacc gccatcggtg ttgtgaacct
     3721 gaaagacggt tgtgtggaga ctatcgccaa ctgggcggcg ttgagggatg ccgccctgct
     3781 cggcctcgac gcggcgtcag ggggcgtgga ccttcacggc ccggcccagt aactgttcgg
     3841 tgctgacttt tccatagacg tagatcgtct cttcgctggt tacgaccccg gccttcaccc
     3901 cgggccgcag tatgacgtct ccgccggcct ggatagagtg gaggcaggcg ttgtcgcaga
     3961 tggtggctct ctcgatcacc cggagcggcc ctttgatcct ggcgtcgtgg ccgatgatgg
     4021 cgctctcggc ctcgacaaac ccgccgacgg tggatccggg gccgagttca agccggccgc
     4081 tgacaaccat gttcccccag atatgcgtcc aggggggagt gataaagttt ccttctatct
     4141 tcacgttccc gtcaaagaag gaacctttag gtgctatgta cgtgtccccg tgccggtaaa
     4201 ccttcatgga gatctcccgg ctaacaactc tcgtccataa ccagataaaa ttatcagcga
     4261 ttgtcagcgc aacaggacgg taacctgccg gaaggagagc accgccggag acccagaaag
     4321 cctccgtcgc cggcgccgtg agagggtgac ggagaggctg tggcgattgc acctggaaag
     4381 ccgtgttacg gggg
//
LOCUS       DAOSZX010000113         4352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_16467_length_4352_cov_18.981150, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000113 DAOSZX010000000
VERSION     DAOSZX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16467_length_4352_cov_18.981150"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(683..1492)
                     /locus_tag="PK196_07575"
     CDS             complement(683..1492)
                     /locus_tag="PK196_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41313.1"
                     /translation="MTFGMSPDYGSSLVEAPGGGYVVATYSGFLTRVDEDGNVLWSTS
                     LGSGSDWWKVVGAPGGGYAVAGDTGIVKVGEDGEIAWTAAFETGLNVGTIAAIPSGGF
                     IAGGITGADVWVARLDAEGNTVWDETLGSRSRDDLYVVRISPAGTYDLVYGTVLDARN
                     ETADGRFTETIEASLAADGRLIGERPLNASRVIVATEDGGYAYAGFIVPEFIGLQPQG
                     YPGSPLHVVRLDDEGMVAWDASYDIGDDRSVVSIIRTSDDGVAVLGSVYDF"
     gene            complement(1873..2820)
                     /locus_tag="PK196_07580"
     CDS             complement(1873..2820)
                     /locus_tag="PK196_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41314.1"
                     /translation="MGKRIGMRALSILLAVMLVSVVVPAVSAWTENDPICIEDRAKVQ
                     GLFEPVDEDIRVIAAENLVVSDRASSVRCSELLSEIMEKYDQNLEDIISILEKATDVQ
                     PDEKDRTVLKEVIVGEHFRRVCDEILMEQRDLREEDAVIVQPQILGVADCQEDMDKML
                     AGTDRWDTFILAQCSPDVYGGIGLDGANLPYLVNGGNNLYEVGVWPGSPTTYQLRYYD
                     EDHPSPVTDIEYDAFRLLYYGTLEDRALFDVTGSQIYFRYCWNDGYTYAWLYGIHGYQ
                     YRPKTSVIYTSNIWNHDIDTYNSNPNIGQLVMSVPYIQQ"
     gene            complement(3058..3789)
                     /locus_tag="PK196_07585"
     CDS             complement(3058..3789)
                     /locus_tag="PK196_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41315.1"
                     /translation="MPRKIGALVCLALAIIARCAWVLGPGQTPANPLPAVAGAGEGTY
                     SLATEFPEVQDSYTLYRVVPVTITEDRVREIAEWFGLSGESKAVSEVTGEFLLVDVSK
                     EPEEQVSVYAHSGAVAYHILDLELPTEVTQQPDLPTNAEAEKIALAFLEKNGMKSPDA
                     RVVKTEVNQKQEVWKSGGSEPEVSYDITKAVRFGRSLDGLPVYGDEFAAILGDGGEVV
                     GLAKTWREVAPDGNVSLRSPEEGKQ"
     gene            complement(4012..>4352)
                     /locus_tag="PK196_07590"
     CDS             complement(4012..>4352)
                     /locus_tag="PK196_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41316.1"
                     /translation="REVYDLYKSRSLVEEHFAVFKSLIQADKLYLRDATAVFGHVFVG
                     FLCLYLYCRILNRIKKAGMTAHLSPHGLLLKLSKVYAVRSGEERAITEVPKQVRKIAE
                     RLELNIFPNV"
BASE COUNT          914 a         1328 c         1139 g          971 t
ORIGIN      
        1 ggttctcgca tcccgccgtc cgcgctcacg caggaaagag ggttccgggg tgagatgagt
       61 cccggcacgt tttttgcctc cgccgggaca acacatcata tatgaccata gacgtcctcg
      121 cgttttcggc ctcgccccgc cgccacgtcc gccggtatcc ttcctggggg cgctggaggt
      181 cgggaagggt gctctcccgc gcatgccgag cacatccccg atggcggcct cgaggaggca
      241 gcctttgaac tgatcgcgca tcttgggtgg taactctcgt acggttgctc ttaagcgttg
      301 ttgttccgcg cgcgcgttac cacgcactgc atgggcaggc gagggagaat cactctcgcc
      361 ggagatctct ttccccggac gcctttttca gcgaccgcaa ggccggggca ctcgggttac
      421 aggcattcag gttgaccggg tgcggttaag ataccctacc cgatcctgca tggtccccgg
      481 catcgtccag tttcagcacc cagacgtccc cctccagcgt gcgatggccc ggggtcagcg
      541 gccgatcgaa gcatccggcg gcgacggtta gcaggatgag cgcggcaacc acttccaagg
      601 tccggatttt cccctgagct gttttcatcg aatcatgctc cttgatgggt tgtaaaaagg
      661 tgtagtatgg aatataaagc gctcagaaat catatacact cccgaggact gcaaccccat
      721 cgtccgacgt ccggatgatc gagacaaccg atctatcatc tccgatgtcg tacgatgcat
      781 cccatgcgac cattccctcg tcatcgagcc tgacaacgtg aaggggggat cccggatagc
      841 cctgcggctg gagtccgata aactcgggaa cgataaaacc ggcatatgca tatccgccgt
      901 cctccgtcgc gacgatgacc cgggaggcat tcaatggtct ttccccgatc agcctgccgt
      961 ccgcggcaag agaggcttcg atcgtctccg taaatcgccc gtcagcagtt tcatttcttg
     1021 catcaaggac ggtgccatag acgagatcgt atgtcccggc aggggagatg cggacaacgt
     1081 agaggtcatc gcgcgaccgg ctcccgagcg tctcatccca gacggtattt ccctctgcat
     1141 cgagtcttgc gacccataca tccgctccgg tgatccctcc tgcgatgaaa cctcccgagg
     1201 gtatcgcggc gatcgtacca acgtttaggc cggtttcaaa cgctgcagtc catgcgatct
     1261 ccccgtcctc gcctaccttg acgatcccgg tatccccggc tacagcatac ccgccgcccg
     1321 gcgccccgac gaccttccac cagtcgctcc cgctgccgag cgacgtactc cagaggacgt
     1381 tcccatcttc atccacccgt gttagaaacc cagaatacgt ggcaacgacg tatccgccgc
     1441 ccggtgcctc gaccagcgag ctgccgtaat ccggcgacat accgaaggtc atggttgata
     1501 tgacattccc gtctctgtcg agagtaacga tgcgggggac cgggccgttc acatcggcgt
     1561 tcgttcccgt gcccgtgatt gcgtacccgc cgtcgcgggt ctgaatcatc gcctgccctc
     1621 tgccgttcgg gtcgccgtcg acgaacgcag tccactggag ggatccgtcc gaattggtct
     1681 tgaggatcca tgtgtccaga gtgaggtctc tatgccccct atcagtgcat ccagcaaaga
     1741 acggtatggt taacgctaaa agaagaatga gtattacata ttttgcactt cttcgggggt
     1801 agtgagaggt tgtggtctcc atgattacag cctgttttcc caatttctca tttactcgaa
     1861 ttaaaaaaga ggttactgtt gaatgtaggg caccgacatg acaagctgcc cgatattcgg
     1921 gttgctgttg taagtgtcga tatcatggtt ccagatattg ctggtgtaga tcacggaggt
     1981 cttgggcctg tactgatacc catgaatccc gtagagccag gcatacgtgt aaccatcatt
     2041 ccaacaatac ctgaagtaga tctgggagcc cgtgacatca aacagggcac ggtcttcaag
     2101 ggtcccgtag tataaaagcc taaaagcgtc atactcgata tccgtcaccg ggctcgggtg
     2161 atcctcgtca taatagcgca gctgatatgt ggtcgggctg ccaggccaga cacccacttc
     2221 atagaggtta ttgcctccat ttaccaagta aggtaggttt gcaccgtcaa gtccgattcc
     2281 gccgtacaca tccggcgagc actgggccag gatgaaggta tcccaccggt ctgttcctgc
     2341 tagcatctta tccatatctt cctgacaatc cgcaacacca aggatctgcg gctggacgat
     2401 cacagcgtcc tcctcccgaa gatcacgctg ttccatcagt atttcgtcgc acactcgtct
     2461 gaaatgctca ccgacaatca cttccttcag aacggttcgg tccttctcgt caggttgaac
     2521 atctgtggcc ttctccagaa tggagataat gtcctcaagg ttttggtcat atttctccat
     2581 gatttccgat aacaattcag aacatctcac tgaactcgca cgatcggata ccaccaaatt
     2641 ctctgcggca atcacacgga tgtcttcgtc gacaggttcg aataatccct ggactttggc
     2701 tcgatcttcg atacagatcg gatcgttttc cgtccaggca ctcactgcag gcaccaccac
     2761 gctcaccagc attactgcca agagtataga gagagctcgc attccgattc tttttcccat
     2821 gttttctacc cctctgctcg ttttcgttac gctaccggcc aggcgtacca gagaaagaaa
     2881 acaacggcaa atgtggcgat aaggaagaga tagaccggac ccagggaaac tacggtctcc
     2941 agggtcacac gaaccgcctg agtaaagccg aattcggtca tgcatgtgct ccggatggcg
     3001 catccgcggg cagatttctc ctatgggcct ttaaaaaccg gggaagaagg gcggatctca
     3061 ctgcttcccc tcctcgggag accggaggct gacattccca tccggtgcca cctcccgcca
     3121 ggtcttcgcg agtccgacga cctcaccgcc gtcgcccagg atcgccgcga attcatcgcc
     3181 gtagaccggc agcccgtcga gcgaccggcc gaacctgact gccttggtga tgtcatacga
     3241 cacctccggt tcgctgccgc cggatttcca gacctcctgc ttctggttca cctcggtctt
     3301 cacgacccgg gcgtccggtg acttcatgcc gtttttctcc aggaatgcaa gagcaatctt
     3361 ctctgcctcc gcgttcgttg ggagatcggg ctgctgggtc acttcggtcg gcagttccag
     3421 gtcaaggatg tggtaggcca cggcgccgga atgtgcatac actgatacct gctcctccgg
     3481 ctccttcgag acgtcgacaa ggaggaactc cccggtcacc tcgctgaccg ctttcgattc
     3541 tcccgagagc ccgaaccact ctgcaatctc ccggacccga tcctccgtga tggtgaccgg
     3601 aaccacccgg taaagcgtat acgaatcctg cacttccggg aactctgtgg cgagagagta
     3661 tgtaccttca cctgcgcccg ccacggcggg aagaggattc gccggggtct ggcccggacc
     3721 aagaacccat gcgcacctgg ctatgatcgc gagagcgaga cagacgagtg caccgatctt
     3781 tcgtggcatt ttaagacctc ctatgtacta tcccgggaga tttcgctgcc cgggacggga
     3841 aatgcatatc ctgaggtatg ggagaaacat acgtttcctt tgcctccggg catgcggggc
     3901 gcgtcatccg ggcgtcagac cagggggtgg gcagcccccg cacacgagcg ggcaccgcac
     3961 gcggcaccca atccaattct gggtcgttag aacatataaa acccaatctc ctcatacatt
     4021 agggaatata ttcaattcaa gcctctctgc gatcttccgt acctgctttg gcacctcggt
     4081 gatagctctc tcctccccgc tcctcactgc atacaccttt gaaagtttca ggaggagacc
     4141 atgcggtgag agatgagcgg tcattcccgc tttcttgatc ctattcagga tcctgcagta
     4201 gaggtagaga cacaggaacc ccacaaagac gtggccaaag accgccgttg catcgcggag
     4261 gtatagttta tccgcctgga ttaaactctt gaacacagca aaatgttctt ccaccaggct
     4321 tctggacttg tagaggtcgt agacctcccg gg
//
LOCUS       DAOSZX010000114         4277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_16802_length_4277_cov_17.630744, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000114 DAOSZX010000000
VERSION     DAOSZX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16802_length_4277_cov_17.630744"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="cofC"
                     /locus_tag="PK196_07595"
     CDS             <1..615
                     /gene="cofC"
                     /locus_tag="PK196_07595"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HON41317.1"
                     /translation="VNSKTRLSCILNQEEREAFARAMLEDVIAAVQKSGCSATLLCTH
                     PFKHDNVLIAVRTEPLNDAINWALRQFHCPALIIMADIPLVTAGDIQRLIRTEKDMAI
                     VPGRGGGTNVIFLKKPQCFHADFYGASFLDHLRVAEECGLSVDVIDSFRMSTDIDEKE
                     DLVEILVHGKGRKSREFLENSGFSLAIDEKGRVGVKRNPHEEAL"
     gene            complement(541..1347)
                     /gene="cofE"
                     /locus_tag="PK196_07600"
     CDS             complement(541..1347)
                     /gene="cofE"
                     /locus_tag="PK196_07600"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HON41318.1"
                     /translation="MTPPSFSVYGIATPLIHTGDDVAERLLESVGRSGAGGFKTGDVL
                     AVAESALATAEGRVVSLASVEPSEEALRLARDYVMDARLAEVVLRESDRVVGGIPGFL
                     LCMKNGTLLPNAGIDASNAPAGSVVLLPLDPDASAARIRSAIAERTGVDLGVIIVDSR
                     THAMRLGCSGVAIGCSGIPSVIDERGRKDLFGRELQVTKRAVADCIASAAELVMGEAD
                     ECVPAAVIRGIGLPIGDYNGVATIDASECLFMGVALHADPSLLVDGKREP"
     gene            complement(1344..2162)
                     /gene="folP"
                     /locus_tag="PK196_07605"
     CDS             complement(1344..2162)
                     /gene="folP"
                     /locus_tag="PK196_07605"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HON41319.1"
                     /translation="MQQHNCLVNGLRIGAGAPVRLMGVINCSPESFYRGSYTPAESVH
                     GRALAMVESGADMVDIGARSTAPGSRPITLAEEAARMDEALRELDGTGVTVSVDVQQP
                     EVLEVCLRHDIHAVNDITGLAVEQFGRAVADSGLPVFVMASLRRPGDAVGLAATLQAL
                     ETVLERCWRFGIEDYVLDPGIGRWVPERTSEDDWELCRNFQSFLDFGRPVLAAVSRKT
                     FIGELLGKPPEDRLAGTLALTTMLVAAGAAVVRGHDVPETADLLRVCAKMRQRG"
     gene            complement(2138..2989)
                     /gene="folD"
                     /locus_tag="PK196_07610"
     CDS             complement(2138..2989)
                     /gene="folD"
                     /locus_tag="PK196_07610"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HON41320.1"
                     /translation="MILDGKAVSEKRLELLKEMIEESGLYPRLATVIVGDDPASQMYV
                     RMKHRACEQVGIGSIGIDLPADASTERVLEAVVKLNNDPDINGILVQLPLPAGVDTGR
                     VINAIAPNKDVDGFHPCNIGRLFSGDPLFAPCTPQGIMTILEEYRIPIAGKHAVVIGR
                     SIDVGRPMAALLLNADATVTICHSKTGDLKEVARRADILVSAVGKARFVGPDMVKEGA
                     AVIDVGINHDEDGKLCGDVDFEAVKDVAGAITPVPGGVGPMTIATLMENTFKAARLET
                     CNSITAW"
     gene            complement(2986..4257)
                     /gene="glyA"
                     /locus_tag="PK196_07615"
     CDS             complement(2986..4257)
                     /gene="glyA"
                     /locus_tag="PK196_07615"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HON41321.1"
                     /translation="MSSLANFDPEIAGVIEKERLRQINGLELIASENVVSKAVLEAMG
                     SIMTNKYAEGYPGKRYYGGCEFHDVAENLARDRMCQVFGAEHANVQPHSGSQANQAVY
                     FAYLGYKDRILSQSLTQGGHLSHGSPVNITGRWYSIFHYGVDPETETLDYAVIEDLAR
                     MVRPQMIVCGASAYPREIDFRAFQEIADTVGARCMADIAHIAGLCATGYHNSPVGVVD
                     VVTTTTHKTLRGPRGGAIMCSKEDAPTIDKSVFPGMQGGPLMHIIAAKAVCFKEAMTP
                     AYKEYCGQIVRNSRAMARVLAEEGLDLVSGGTDNHLILLDLTGVSANHEHLTGLAAET
                     ALGEAGITVNKNTIPREQLSPFVTSGLRIGTPAVTSRGMKEKEMEQIAYWIATVVKDI
                     ARDRTSKKAINEVREEVIALASNYPLYPEVA"
BASE COUNT          786 a         1416 c         1252 g          823 t
ORIGIN      
        1 gtgaactcga agacgcgcct atcctgcatc ttaaaccagg aagagcggga ggcgttcgcc
       61 cgggcgatgc ttgaagacgt gatcgccgcc gtgcagaagt ccgggtgcag cgccaccctg
      121 ctctgcaccc acccattcaa acacgacaac gtgctcatcg ccgtccggac ggaacccctc
      181 aacgatgcca tcaactgggc gctccgccag ttccactgcc ccgcgctcat catcatggcc
      241 gacatacctc tggtcacggc cggggatatc cagcgtttga tccggacgga gaaggatatg
      301 gccatcgtcc ccggccgcgg cggaggaacg aacgtgatct tcctcaagaa accgcagtgc
      361 ttccacgccg acttctacgg tgcgagtttc cttgaccacc tgcgggttgc agaagagtgc
      421 gggttatccg tcgatgtcat cgactcgttc cggatgtcca ccgacatcga cgagaaagag
      481 gacctcgtag agatcctcgt ccacggaaaa gggagaaaga gcagagagtt cctggagaac
      541 tcagggttct ctcttgccat cgacgagaag ggacgggtcg gcgtgaagcg caacccccat
      601 gaagaggcac tctgaagcgt ctatggtggc aaccccgttg tagtcgccga tggggaggcc
      661 gatcccccgt ataaccgccg ccggaacaca ctcatcagcc tcccccataa ccagttcagc
      721 ggccgaggct atacagtccg caaccgccct ttttgtgacc tggagctcgc ggccgaatag
      781 atcctttctc ccgcgctcat ctatcaccga tgggatcccc gaacacccga ttgcaacccc
      841 gctgcagcca agccgcatcg cgtgcgtccg ggaatcgaca atgatgacac ccagatcgac
      901 ccccgtccgt tcagcgatcg cggatcgtat ccgcgccgcc gaggcgtcag ggtcgagggg
      961 caggagaaca accgaaccgg cgggagcgtt tgaggcgtcg atccccgcgt tcggtaggag
     1021 cgtcccgttc ttcatgcaga gcaggaaccc gggtatcccg cccacgaccc ggtcgctctc
     1081 ccggagcacc acctcggcga gacgggcgtc catcacgtaa tcacgggcga gccggagcgc
     1141 ctcttctgaa ggctcgacgc tggcgagact cacgacccgc ccctccgcgg ttgccagcgc
     1201 cgattccgca accgccagaa catcaccggt cttaaacccc cccgcgcccg accgccccac
     1261 ggactcaagg agcctctccg ctacatcatc ccccgtgtgg atcagcgggg tcgcgatccc
     1321 gtagaccgag aaagacggag gcgtcatcct ctctgcctca tctttgcgca gacccgcagg
     1381 aggtcagcgg tctccgggac atcgtggccc cggacgacgg ccgcacccgc ggcgaccagc
     1441 atcgtcgtga gcgcaagggt gcccgcaagc cggtcctccg gcggtttccc gaggagctcc
     1501 ccgatgaacg tcttacggga gaccgcggca agaaccggcc ggccgaagtc caggaacgac
     1561 tgaaaattcc ggcagagttc ccagtcatcc tcgctcgtcc gctccggcac ccatcgccct
     1621 atacccgggt caagcacgta atcctcgatc ccgaaccgcc agcaccgttc gagaaccgtt
     1681 tcaagcgcct gaagggttgc agcaagcccc acggcatcgc ccggccggcg gagacttgcc
     1741 atcacaaaga ccggcagccc cgagtcggcg acggcccgtc caaactgctc tacggcaagc
     1801 cccgtgatat cgttcacggc atggatatcg tggcggaggc agacctcgag cacctcgggc
     1861 tgctggacat ccaccgagac ggtgaccccc gtcccgtcca gttcccggag cgcttcgtcc
     1921 atacgggcag cctcctctgc gagggtgatc ggacgagacc ccggcgccgt gctccgcgcc
     1981 ccgatgtcga ccatatcggc gccggattcg accattgcaa gggcacggcc atgaacactc
     2041 tccgccgggg tgtaggagcc gcggtagaac gattccgggc tgcagtttat gacgcccatc
     2101 aaccgaaccg gggcacccgc gccgatccgc aggccgttca ccaggcagtt atgctgttgc
     2161 atgtttccag cctggcggcc ttgaacgtgt tctccatcag cgttgcgatg gtcatggggc
     2221 cgacgccgcc cggaacaggg gttatagcac ccgccacgtc cttcaccgcc tcaaagtcga
     2281 catccccgca gagttttcca tcttcgtcgt ggtttatgcc gacatcgatg accgccgccc
     2341 cctccttcac catatcgggc ccgacgaacc ttgccttccc caccgcgctg acaaggatat
     2401 cggctctccg cgccacctcc ttgaggtctc ccgtctttga gtggcagatg gtgacggttg
     2461 catcggcgtt gaggagcagg gcggccatgg gtcttcccac atcgatgctc cggccgatga
     2521 cgaccgcgtg ctttcccgcg atagggatgc ggtactcctc caggatcgtc atgatcccct
     2581 gcggcgtgca gggcgcaaag agcgggtctc cagagaatag cctgccaatg ttgcaggggt
     2641 ggaacccgtc gacatcctta ttgggtgcga tcgcgtttat cacgcggccg gtgtccacac
     2701 cggccgggag cggcagctgg acaaggatgc cgttgatgtc cgggtcgttg ttcagtttca
     2761 cgaccgcctc gagcacacgc tcggtggagg cgtccgccgg gaggtcgatc ccgatcgatc
     2821 cgatccccac ctgttcgcac gcccggtgct tcatccggac gtacatctgt gatgccgggt
     2881 catcgcccac gatgaccgtt gccaggcgcg ggtagagccc ggactcctct atcatctcct
     2941 taaggagttc aagcctcttc tcagagactg ctttgccgtc gagtatcatg ctacttcagg
     3001 gtacagcggg tagttgctcg cgagagcgat gacctcttct ctcacctcat tgattgcctt
     3061 ctttgaggtc ctgtctcttg cgatgtcctt taccaccgtc gcgatccagt aggcgatctg
     3121 ttccatctcc ttctctttca tgccgcggga ggtgacggcc ggtgtgccta tacgcagccc
     3181 gctcgtcaca aaggggctga gttgttcgcg tgggatggtg ttcttgttca cggtgatccc
     3241 ggcctcgccg agcgcggtct cggctgcaag cccggtgagg tgttcatggt ttgccgagac
     3301 cccggtaagg tcaaggagta tgagatggtt gtcggtgccg ccggagacca gatcgagccc
     3361 ctcctctgca aggaccctgg ccatcgcgcg cgaattcctg acgatctggc cgcagtactc
     3421 tttgtatgcc ggggtcatgg cctccttgaa acagaccgcc ttcgcggcga tgatgtgcat
     3481 cagggggccg ccctgcatcc ccgggaagac ggacttgtcg atggtcggcg catcctcttt
     3541 gctgcacatg attgcaccgc cccggggacc gcgcagcgtc ttatgagtag tcgtcgtcac
     3601 tacatcgacg acacctaccg gggagttgtg gtatccggtt gcgcagagtc cggcaatgtg
     3661 ggctatatcg gccatgcacc gcgcaccaac ggtatcggcg atctcctgga acgccctgaa
     3721 atctatctcg cgcgggtagg cgctcgcgcc gcagacgatc atctgcggcc tgaccatccg
     3781 cgccaggtcc tcgatgacag cgtagtcaag cgtctccgtc tccgggtcca cgccgtagtg
     3841 aaagatggag taccagcggc cggtgatatt taccggcgac ccgtgggaga ggtggccgcc
     3901 ctgggtgagg ctctggctca atatcctgtc cttgtagccc aggtaggcaa agtaaaccgc
     3961 ctggtttgcc tggctgcccg agtgaggctg gacgtttgcg tgctcggccc cgaagacctg
     4021 gcacatccgg tcgcgcgcca ggttctctgc gacgtcgtga aactcgcagc ctccgtagta
     4081 tcgtttgccg gggtaaccct cggcgtattt attggtcatt atagaaccca tcgcctccag
     4141 gactgccttg cttacgacgt tctcggaggc gatcaattcc agcccattga tctgacgcag
     4201 gcgctctttc tcgatgacac ctgcgatctc tggatcaaag tttgccagac tagacatgca
     4261 gaaaaatggt tgctccg
//
LOCUS       DAOSZX010000115         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_17410_length_4154_cov_20.142718, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000115 DAOSZX010000000
VERSION     DAOSZX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17410_length_4154_cov_20.142718"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..249
                     /locus_tag="PK196_07620"
     CDS             73..249
                     /locus_tag="PK196_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON41322.1"
                     /translation="MLRINRDKCGYCGTCVAVCPEDALELIDAYLSLEGECIACGICA
                     RACPLGALEVSNEE"
     gene            239..1435
                     /locus_tag="PK196_07625"
     CDS             239..1435
                     /locus_tag="PK196_07625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON41323.1"
                     /translation="MKSKYDILVIGGGPAGALAAKTAAKAGNSVCLIEKRPAIGTPVR
                     CAEGIGKDLLKEYVKPDPRWISADIERARIIAPNGTTISLEQDKAGNEVGYVLDRKIF
                     DRELVWQAAEAGADVFVKTRATAPIMENGAVRGANVISIGTPAEIRAEVVIAADGVEA
                     RFARRAGLETTVPLREMMSCAQYLMTDIDIDAGSTDFYLGNAIAPEGYLWIFPKGERT
                     ANVGIGISGRRSRDGSRAKDYLDRFVAKNFPDGKMIEAIVGGVPVCRPLECTVADGLM
                     VVGDAARVVDPITGGGIGNALFTGRLAGQVASDCIAAGDCSKEALMAYDTGWRTSKMG
                     ASIERNYKIKEYFVTLDDAKLNTLADSIASASLKEFSVRAIIKELIKRNPKMILELKA
                     LRDALS"
     gene            complement(1427..2092)
                     /locus_tag="PK196_07630"
     CDS             complement(1427..2092)
                     /locus_tag="PK196_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HON41324.1"
                     /translation="MYTIIVGLGGIGRSLTAIAVNAGDSVVVIDQNEERASDILEHYD
                     VLAIIGNATDKSVLEDAGIDRADALVATTSDDAVNLMTCWLAKRYNVRNVVSIVNQKE
                     HSDLFKEVGVRISENPDELVATRLYYWTKSPNLQQVASIPGGTIFEIVAEEGAPIVDH
                     EIRELDVRDFVFIAIRRAGGDLIIPSGTVRIRPGDVITVFTKKEAEAETLKTLNKQLT
                     RSG"
     gene            2116..2775
                     /gene="rnhB"
                     /locus_tag="PK196_07635"
     CDS             2116..2775
                     /gene="rnhB"
                     /locus_tag="PK196_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HON41325.1"
                     /translation="MKYLANKSECVICGVDEAGKGSVLGPMVVAAVGCQRVDDLADLP
                     IRDSKALRPRQREALYDLLTRKFSIAIASIDAPGIDEARSRMSMNTCVAELHADVIRR
                     LRPDCAYVDACDVNADRYGRTVAALLDFPCDVVAEHRADARCIIVGAASIVAKVTRDR
                     AIEELEERYGKIGSGYPSDPATVEFLRGYIRDHGTPPPCARRTWKTVANLSQRTILDF
                     S"
     gene            2817..4151
                     /locus_tag="PK196_07640"
     CDS             2817..4151
                     /locus_tag="PK196_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HON41326.1"
                     /translation="MTWIQILLLIVAAYLAIALYIRKRGLFKDHVMFFGPILAIKTER
                     VEFFDWFRKFATPLRAYATLGVVMVVVVSVFMTLALILTVPQYLVHTPEPTGIYDPRN
                     LLAIPGLNQAIPITAAVILGLLLTIVIHEFGHAILARIEDMRVRSMGLLIAVVPIGAF
                     VEPDEEDVERARGMPKIRMFGAGITNNIVFGLLCFAVMLLLIGMATPLPIPLVQGVYQ
                     DYPAAEAGIQGYSVITEINGIPVKTQEEIAAIMEGTRPGDTITLTAVKDGVESTCTLT
                     LEEWPEVLGGDRDSGFMGVYYYSAPAVKERLDRIADLGLLAPLYLTIVPIDAFIQGNT
                     MQLAILLADTPEQMAWEEPFPLFWGLVRLLFWTGWFNLLVGMFNAIPIVPLDGGYMMK
                     EGVERLFERLGWSQQYAQRVVASISGFVTVMLILLITMPLIGAAVRSVLAMG"
BASE COUNT          838 a         1234 c         1268 g          814 t
ORIGIN      
        1 tcgaggcgcc ggtgcagcaa ctgaagcaag gagcataaag gtttttgggg gacaggcgat
       61 attatgagta caatgctcag gataaaccga gacaagtgtg gatactgcgg cacctgcgtc
      121 gcagtctgcc ccgaggatgc gcttgagctg atcgacgcct acctcagcct ggagggtgag
      181 tgcatagcct gcggtatctg cgccagggcg tgcccgctcg gagcactgga ggtcagcaat
      241 gaagagtaaa tacgatatcc tcgtcatagg gggcgggcct gcaggggctc tcgcggcgaa
      301 gacggcagcg aaagccggga actcggtctg cctcatcgag aaacgtcccg caatcggcac
      361 ccctgtccgc tgtgcggagg ggatcggcaa agatctcctg aaagagtatg taaaacccga
      421 cccccgctgg atatccgccg atatagagcg cgccaggata atcgccccga acggaacaac
      481 aatcagcctc gaacaggaca aagccggaaa cgaggttggt tacgtcctcg accgcaagat
      541 attcgacagg gaactcgtct ggcaggcggc cgaggccggt gctgacgtgt tcgtcaagac
      601 acgggcaacc gcgccgatca tggagaacgg cgcagtccgg ggcgcaaatg tcatctcgat
      661 cggcacgccg gcagagatcc gggctgaggt ggtcatcgcc gccgacggcg tcgaggcgcg
      721 gttcgcgcgc agggccggac tcgagaccac cgtacctctc cgggagatga tgagctgcgc
      781 ccagtatctt atgaccgata tagatatcga cgccggttca acggacttct accttggcaa
      841 cgcgatcgcg cccgaaggct acctctggat cttcccgaag ggtgagagaa cggcgaacgt
      901 cgggatcggc atctccggcc gcaggagcag ggatggatcg agggcgaagg actacctcga
      961 ccggttcgtt gcaaagaact tccccgacgg aaagatgatc gaggcgatcg ttggcggcgt
     1021 ccccgtctgc cggccgcttg agtgcacggt cgccgacggg ctcatggttg tcggggacgc
     1081 ggccagggtg gtcgacccca tcaccggcgg cgggatagga aacgccctct tcaccgggag
     1141 gcttgccggc caggtcgcat cagactgcat agccgcgggc gactgttcga aggaagcgct
     1201 gatggcatac gacacgggat ggcgcacctc aaagatgggc gcatcgattg agaggaacta
     1261 caagataaaa gagtattttg tcacccttga cgatgcaaaa ctcaacaccc tggcggactc
     1321 gatcgccagc gcgagtctca aagagttctc tgtacgggcc atcatcaaag aactgatcaa
     1381 gcgcaacccg aagatgatcc ttgagctgaa agcgctcagg gatgccctat cctgagcgcg
     1441 tcagttgttt attgagggtc ttcagggtct ccgcctccgc ctcttttttc gtaaacaccg
     1501 ttatgacatc cccgggacgg atacggacgg tgccgctcgg gatgatgaga tcccctccgg
     1561 cgcgcctgat ggcgatgaag acaaagtccc gaacatcgag ttcacggatc tcgtgatcga
     1621 ctattggcgc cccctcctcg gcgactatct caaagatggt gccgccaggg atcgaggcta
     1681 cctgctggag gttcgggctc tttgtccagt agtataaccg ggttgcaacc agttcgtcag
     1741 ggttctcgct gatcctgacg ccgacctcct tgaagagatc ggaatgctcc ttctggttga
     1801 cgatcgagac gacgttcctg acgttgtagc gttttgcaag ccagcaggtc atcaggttca
     1861 cggcgtcatc gctcgttgtg gcgacaagag catcggcacg gtcgatcccc gcgtcctcga
     1921 gcaccgattt gtctgtcgcg ttcccgatga tcgcaaggac atcgtagtgc tccaggatat
     1981 cgcttgcacg ttcttcgttc tggtcgatca ccaccaccga atcaccggcg ttgacagcga
     2041 tcgcggtcag gctccggccg atgccaccca ggccgacgat gatcgtatac atccatctgg
     2101 attcaaccct cagcattgaa ataccttgcg aataaatcag aatgcgtgat ctgcggggtc
     2161 gatgaagcgg gaaaaggttc ggttctcggg ccgatggtgg tcgcggccgt ggggtgtcag
     2221 cgagtcgacg accttgcaga cctccccatc cgggactcaa aggccctccg gccccggcag
     2281 cgtgaggcgc tgtatgatct cctgacccgg aagttctcga ttgccatcgc cagcatcgat
     2341 gcccccggta tcgatgaggc ccggagccgg atgagcatga acacatgcgt ggccgaactc
     2401 catgccgatg ttatacggag actccgcccg gattgcgcct acgtggatgc ctgtgatgtc
     2461 aacgctgacc gatacggccg gacggtggcc gcactcctgg acttcccctg tgatgtcgtc
     2521 gccgaacacc gggcggatgc ccgttgcatt atagtcggcg cggccagcat agtcgcaaag
     2581 gtcacccgtg accgcgcaat agaggagctt gaggagcggt acgggaaaat cgggagcggc
     2641 tacccctcag accccgcaac cgtcgagttc ctccgcggct acatccgtga tcacgggacc
     2701 ccgccgccct gcgcccgcag gacatggaag acagtcgcaa acctctccca gagaacgatc
     2761 ctggactttt cgtgacctgg aaggatttat gcctcgcgcg ccacaaccct tgagtgatga
     2821 cctggatcca gatactcctg ctcatcgttg cggcatacct ggcgatagcc ctctacatcc
     2881 ggaagcgggg gctattcaag gatcacgtca tgttctttgg cccgatcctg gccatcaaga
     2941 ccgagagggt cgagttcttc gactggttcc ggaagtttgc aacaccgctc agggcctacg
     3001 ccaccctggg tgtcgtgatg gttgtcgtgg tctcggtctt catgacgctt gccctcatcc
     3061 tcaccgtacc ccagtacctg gtgcataccc ctgaacctac agggatatac gacccccgca
     3121 acctccttgc gatacccggt ctcaaccagg cgatcccgat cacagcggcc gtcatcctgg
     3181 gtctcctcct cacgatcgtc atccatgagt tcgggcatgc gatccttgcc cggatcgagg
     3241 atatgcgggt gcggagcatg ggtctcctca tagccgtcgt cccgataggg gcgtttgtcg
     3301 agcccgacga ggaggatgtt gagagggccc gggggatgcc caagatccgg atgttcggcg
     3361 ccgggatcac gaataacata gtcttcggcc ttctctgctt tgcagtgatg ctccttctca
     3421 tcgggatggc gacaccgctc ccgatacccc tggtgcaggg ggtctaccag gactaccctg
     3481 cggcagaggc cgggatccag ggctactcgg tcatcacgga gataaacggc atccccgtga
     3541 agacccagga agagatagcc gcgatcatgg aggggacgcg gccgggggat accattaccc
     3601 tgacggccgt aaaagatggg gttgagagca cctgcaccct caccctggaa gagtggccgg
     3661 aggtgctcgg tggtgaccgg gactccgggt tcatgggggt ctactactac agtgctcccg
     3721 cagtgaagga gcgtcttgac aggatcgcgg atctcgggct gcttgcacct ctctacctga
     3781 ctatagtccc gatcgacgct ttcatccagg gcaacacgat gcagcttgcc atcctgcttg
     3841 cagacacccc cgaacagatg gcctgggagg aaccgttccc cctcttctgg gggctggtcc
     3901 gcctcctctt ctggacaggc tggttcaacc tgcttgtcgg gatgttcaac gccataccga
     3961 tcgtcccgct cgacggcggt tacatgatga aggagggggt tgagcggctc ttcgagcgtc
     4021 tgggctggtc acagcagtac gctcaacgtg ttgtcgcctc gatcagcggg ttcgtcacgg
     4081 tgatgctgat cctcctcatc acgatgccgc tgatcggcgc tgcggtgcgc tcggtgctgg
     4141 cgatggggta aata
//
LOCUS       DAOSZX010000116         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_18556_length_3940_cov_22.072844, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000116 DAOSZX010000000
VERSION     DAOSZX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18556_length_3940_cov_22.072844"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..626
                     /locus_tag="PK196_07645"
     CDS             141..626
                     /locus_tag="PK196_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HON41327.1"
                     /translation="MISIYINYGIWYAILLAICAFLTTFSQNLSDPYYEIARLALLGV
                     PAAVLVYVVVAPPVFYTRYRYRITDDRVEVRRGVLVIRHTLVPIERVHQVEITRGPIK
                     NLLGLADVAITTAGGVATINYLELEEAERVAELLNKLIGEMLRERLQAPASSVTGSAD
                     E"
     gene            623..2212
                     /locus_tag="PK196_07650"
     CDS             623..2212
                     /locus_tag="PK196_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HON41328.1"
                     /translation="MTGRDRETPARSSAAREQSTDDRAAADHPGTASRTGEMEIWGKR
                     VRCHPGIIVEKTLPFAPILFILIITLVGRPAAAAALAVLFIAVVFINYRIWSRTTIQF
                     DDTGVVVERDTVFKRKKTIPYSKVASVNINRGITNRLLGTSSLLININSGSGATVPEA
                     VLTFEQDTAESIRSGILHRLYTHSKDQDEGDEPVEMPALFSPLDVILHGLFSVSTYQT
                     VSGLFFLVYSVFQFYVSAGTDGGSAGAVVSLLIFAALLVGPIISQIFYYYNYRVYRRG
                     DTIYLQHGLIRSYRTSFDVSRINAVRVKSTLAARLMHRSCIEAEVVGLASGKGQSLRP
                     VLCLLKDDATQQTLLRELVPEFVYDRKPEKQPEGAARVLLIEAGAASLALALVMIYPS
                     IYLYRRAMTATGIAGLTMPYMLPVITVSLILAIFYAVYVLYRITDLDLGENLFSFVNG
                     AVDRETVTMNYDRVQMVVTAKGPIARLFDVARSNIYLLSSIGGSSITSGYFHERDLNA
                     IGEIVMERIASGDYDYRKNSL"
     gene            complement(2237..3319)
                     /locus_tag="PK196_07655"
     CDS             complement(2237..3319)
                     /locus_tag="PK196_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HON41329.1"
                     /translation="MPVMKIRGGDLDLIEYEFTSFSPGENIRTCGLQKDYRLTPVSGT
                     VTVRAPARIHLAVLDMNRFAPDRPGGGGIGFAISVYCTAEVECTDSGTEIDYSREPIL
                     RHFTEVFRQVVGYKGGFKIHARDHHYEHVGLGSTSTILIAVANALNAAVGSPLTSDNL
                     RLLLGSNFVEETVAGNVAFGFETGVGPAASTYGGMAVMGDKLTLIYRHAFAEGKRVYI
                     VIPPSEISSSGEKEFNLLMNKARTLDYRDRELKSYLVMMDLIPALECGDLEKIGEVVW
                     EIEFRGSKRAEVEHHGFEIYRYMAALRGAGLEFVGMSSVGPAIAVITGRPEKEVAEVL
                     ADLGLRIAIATTVDNEGLKISVERRA"
     gene            complement(3332..3883)
                     /locus_tag="PK196_07660"
     CDS             complement(3332..3883)
                     /locus_tag="PK196_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HON41330.1"
                     /translation="MNYESDAVEMVARLMALSARTAPKATGTDVIKTMIVTGEEKMRL
                     AAAMRDYGEKHKTGFFIRDAGNVAASDACLLIGSLLQESVGLNCGACGYATCAEMLDA
                     QRERSHTATPFQGPNCAVRMADLGIAVGSAVKTASIHNVDNRVMYSVGVGALALGWLE
                     GCGVAYGIPLRASGKNIFFDRAR"
BASE COUNT          810 a         1180 c         1129 g          821 t
ORIGIN      
        1 tcttaaagtc ataactgact ttttatcagg ggaattaaat ggctttattt accggacagt
       61 gcgggaccga ggatacggca cccgatccat cctcaggtgc acctggcaca gacggttaca
      121 ggagattgaa ccggcggtgc atgatctcga tctacatcaa ttacggcata tggtatgcga
      181 tcctccttgc gatctgcgct tttcttacga ccttttcgca gaacctctct gacccctatt
      241 acgagatcgc ccgacttgcc ctgctggggg ttccggcggc cgtcctggtg tatgtggtgg
      301 tggccccgcc ggtcttttac acacgatacc gctacaggat caccgacgac cgggtcgaag
      361 tgcgtcgcgg cgttctggtg atacgccata ccctggtgcc gatcgagagg gtgcatcagg
      421 tggagatcac caggggcccg atcaaaaacc tgctcggtct tgcagacgtt gccatcacca
      481 ccgcaggagg cgtggccacc atcaattacc tggagcttga ggaggcggag agggttgccg
      541 aacttctcaa caaactgatc ggggagatgc tgcgggagag gctgcaggcg cccgcatcat
      601 cggtgacggg gtcagcagac gaatgaccgg aagagatcgc gagaccccgg ctcgcagcag
      661 cgccgcgaga gagcagtcga ccgatgatcg agctgctgca gaccatcccg gcaccgccag
      721 ccgcacgggt gagatggaga tctgggggaa acgggtccgg tgtcatccag ggatcatcgt
      781 tgaaaaaaca cttccattcg ctccgattct gtttattctc atcatcacgc tggtcggcag
      841 accggcggca gcagctgcac ttgcggttct attcatcgcg gtggtgttca tcaactacag
      901 gatctggagc cggacaacca tccagtttga cgataccggc gtggttgtgg agcgggatac
      961 cgtctttaag aggaagaaga caatccccta ctcgaaggta gcatcggtca acataaaccg
     1021 tggcattaca aacagactgc ttggaacatc gagtctgttg atcaacatca actctgggag
     1081 cggtgccacg gtgccggagg ccgtcctgac gttcgagcag gacacggcgg agagcatacg
     1141 ttcggggata ctgcaccgcc tctacaccca cagcaaggat caggatgagg gggatgaacc
     1201 ggtcgaaatg ccagcattgt tctccccgct ggatgtgatc ctgcacggtc tcttcagcgt
     1261 atcgacctac cagaccgtct ccggtctctt cttcctggtc tactccgtct ttcagttcta
     1321 cgtctctgcg ggaaccgatg gcggtagcgc cggggcagtg gtctcgctcc tgatctttgc
     1381 cgccctgctg gtgggcccca ttatctcaca gatcttttac tactacaact accgggtcta
     1441 ccgcaggggt gatacgatat acctgcagca cggactgatc cgtagttaca ggacgtcgtt
     1501 tgacgtctcc aggatcaacg ctgtccgcgt caagagcacc ctcgcggcca ggctgatgca
     1561 caggtcatgc atcgaggctg aggtggtggg acttgcgtcc gggaaagggc agagcctgcg
     1621 ccccgttctc tgtctcctga aggacgatgc aacgcagcag acccttctcc gggaactggt
     1681 gccagagttt gtctacgacc gcaaaccaga gaaacagcct gaaggcgcag cgagagtgct
     1741 cctgatcgaa gctggtgcgg cttctctcgc cctcgctctg gtgatgatct atccctccat
     1801 ctacctatac cgcagggcta tgaccgcgac cgggatcgcc gggcttacca tgccctatat
     1861 gctgcccgtg atcacggtct cgctcatcct ggcgatattc tatgccgttt acgtcttata
     1921 caggatcaca gacctcgatc tcggagagaa cctcttctca tttgtgaacg gcgcagtcga
     1981 ccgcgagacc gtcacgatga actacgacag ggttcagatg gtggttactg ctaaaggccc
     2041 gatcgccagg ctattcgacg ttgccaggag caacatctat ctgctctctt ccataggggg
     2101 ttcaagcata acctccggtt atttccacga gagagaccta aacgccatag gcgagatcgt
     2161 gatggagagg attgcaagcg gtgactacga ttaccggaag aacagtctct gaggggggac
     2221 tgtgagggta gcctcctcac gccctcctct ccacgctgat ctttaaccct tcgttatcaa
     2281 ccgtggtcgc gatggcgatc cggagaccga gatctgccag gacctccgcc acttccttct
     2341 ccgggcgacc ggtgatgacg gcgattgcag ggccgaccga actcatgccg acgaactcga
     2401 gccccgcgcc ccggagcgct gccatgtagc ggtagatctc aaacccgtgg tgctccacct
     2461 cggcgcgctt ggagccgcgg aactcgatct cccagacgac ctcaccgatc ttctccaggt
     2521 cgccacattc cagcgccggg atgaggtcca tcataaccag gtatgacttc agctcgcggt
     2581 cgcggtagtc gagcgtccgg gccttgttca taaggaggtt gaactccttc tcccccgatg
     2641 aggagatctc agagggtggg atgacaatat agacccgttt tccctccgca aaggcgtgcc
     2701 ggtagatgag cgtcagttta tcgcccataa ccgccatccc gccgtaggtg ctcgcagcgg
     2761 gtccgacccc cgtctcgaac ccgaacgcca cgttcccggc caccgtctcc tcgacgaagt
     2821 tgctgccgag gaggaggcgg aggttgtcgc tcgtcagggg tgaccccacc gccgcgttga
     2881 gggcgtttgc aacagcgatc aggatggtgc tcgtggaccc gagcccgacg tgttcgtagt
     2941 ggtggtcgcg tgcatggatc ttaaatccgc ccttataacc tacgacctgc cggaatacct
     3001 cggtgaagtg ccgcaggatc ggttcgcgtg agtagtcaat ctcagtccca gaatcggtgc
     3061 actcgacctc ggctgtacag tagacgctga tggcaaaccc gatcccgcct ccaccaggac
     3121 ggtcgggggc gaaccggttc atgtcgagca cggcgaggtg aatcctcgcc ggggcgcgta
     3181 cggtgaccgt accggagaca ggggtgagcc ggtaatcttt ctggaggccg cacgtccgga
     3241 tgttttcacc cggggagaag gaggtgaact catactcgat gaggtcgagg tccccgcccc
     3301 ggatcttcat aacaggcatt ctgctaccgg gttagcgtgc gcggtcaaag aagatattct
     3361 ttccagatgc cctgagcggg atgccgtagg ccaccccgca cccctccagc cagccgagcg
     3421 cgagcgcccc cacaccaact gagtacatga cccggttatc gacgttgtgt atgctcgcgg
     3481 tctttaccgc agaaccgacc gcgatcccga ggtcagccat cctgacggca cagttcgggc
     3541 cctgaaatgg agttgccgta tgagatctct cgcgctgcgc atcgagcatc tccgcgcagg
     3601 tggcataccc acacgcaccg cagttcagcc ccacactctc ttgcagcagc gaaccgatga
     3661 gaagacatgc gtcgcttgct gcgacgttcc ccgcatccct tatgaagaac ccggttttgt
     3721 gcttctcgcc gtagtcccgc atcgctgcgg caagccgcat cttctcctca ccggtaacga
     3781 tcatcgtctt gatgacgtcg gtgccggtcg ctttcggggc agtgcgagcg gatagcgcca
     3841 taagacgggc aaccatctct acagcgtctg attcataatt catgagatta cgtggcaccg
     3901 cctcaagata agtgtatgca caagttaacc gaaatacaga
//
LOCUS       DAOSZX010000117         3883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_18903_length_3883_cov_20.166928, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000117 DAOSZX010000000
VERSION     DAOSZX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18903_length_3883_cov_20.166928"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..985)
                     /gene="speB"
                     /locus_tag="PK196_07665"
     CDS             complement(134..985)
                     /gene="speB"
                     /locus_tag="PK196_07665"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HON41331.1"
                     /translation="MQDPVHLHFADADTPYEDARYAIFGVPYDGTTSFRPGTRFGPRA
                     IREFSFNFEAYDPAAGIELFDVPVTDLGDLMVSRLPEAVVDEVADFVRGIVRDGKVPV
                     MLGGEHTVTVGAVRAVEPEVYVVCDAHLDLRDELDGTPYSHGCVTRRVLETVEDVVII
                     GARSGDREQFEIAAEKTHLYTADVVRERGIAAILGEVMGLIEGRRVYLSIDADAIDCC
                     LTPGLGTPEPFGMTPLDLREVVRTLGPHAAGFDYVEVAPFDAGQTAAVAALLVRQFIA
                     RHWTGRC"
     gene            complement(1007..1384)
                     /locus_tag="PK196_07670"
     CDS             complement(1007..1384)
                     /locus_tag="PK196_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HON41332.1"
                     /translation="MKEQTEVGKLKEGRYVVVDDEPCRIVSIAISKPGKHGAAKSRID
                     CIGVFDGVKRSIVQPVSAKIYVPVVERKIAQVISIAGTTVQLMDVKDFEMFELNVTAE
                     EAAGLEPGKEVAYIASLGKKKLE"
     gene            1473..2267
                     /locus_tag="PK196_07675"
     CDS             1473..2267
                     /locus_tag="PK196_07675"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HON41333.1"
                     /translation="MRRFIRACDDLAAAVRDAVADMVGTPGAGDCVGMGADGTPTKKI
                     DQVAEDIVVDYLADHPLCQRLISEELGCAAMGGNRGTIFLDPVDGTYNAVVGIPFYAI
                     SIAYAADGMVTAGYVQNLATGETFHAIRGEGAFRNGRPIHVSNVSRLEESAMSVYGSR
                     MPDPAPIQRIDRTIRRCRLLGASALELCYVGCGRIEGFVDLRGTLRVTDAAAGMLICK
                     EAGGMVSDPGGGEVVFPDDVSAGRRLVATNGILHSRLIEYLKGNRT"
     gene            2264..3076
                     /locus_tag="PK196_07680"
     CDS             2264..3076
                     /locus_tag="PK196_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HON41334.1"
                     /translation="MRITLVSRLDDAEALRYTASLGRELKDLGHDVALEEGTARHLRR
                     HEGTPFEEIDADLVVVVGGDGSVLLTVQRMRKQVPILGINWGEVGFLTDLEPGEAIDF
                     FAGLDAFEVEQRMRVRLSAGGSVLGDALNEGLIVTDRPAKMLRFGVYVDGTLSERFRA
                     DGLLVSTPTGSTAYAMSAGGPIVDPQIEGILLVPLAPYMLSSRPHIISTRRRLEITLE
                     TDKPAHLVIDGQTTLELPKEATLTVKKSDQPALFVNTGKQFFEKVNRKLRNL"
     gene            3152..3868
                     /locus_tag="PK196_07685"
     CDS             3152..3868
                     /locus_tag="PK196_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HON41335.1"
                     /translation="MEDQMRTKIPYAEIAEMLKRSLNLRGSPVAVKLARKPEDIPDGV
                     GPIEETVRHCQMVSRARLKGEIFYATTERHACMGGAWALGLREITESLSTGEFYYKLG
                     KFETWAACMRTIQQVPHLPEPTTYATVYAPLEKTPFDPHVVLIIAEPRAMLKLAQAEL
                     YLLGGRIESSMAGIQSVCADATALPYLSGKVNYSLGCDGSRRFSGIEDNELVMGIPAE
                     LLPEFARALPIVTGAPGSVK"
BASE COUNT          784 a         1260 c         1159 g          680 t
ORIGIN      
        1 gcgggagcca accgcccggc ttgccgcccc cgcgcggcca taatcaaaaa tgacgtggtg
       61 ggcgccggga ctcaattcgg tctggacggg gtggcggcgc ctgatcgaga cctcaggccc
      121 caccaagccc tttctaacac ctgccggtcc agtgccgcgc gataaactgc cgcaccagca
      181 gcgccgccac cgccgccgtc tgcccggcat caaacggcgc aacctccaca taatcaaacc
      241 ccgcggcatg cggcccaagc gtgcggacga cctcccggag atcgagcggc gtcatcccga
      301 acggttccgg cgtcccgaga cccggcgtga ggcaacagtc gatcgcgtcg gcatcgatcg
      361 agaggtagac cctccgcccc tcgatcagcc ccatgacctc gccgaggatt gcagcgatcc
      421 cgcgctcccg caccacgtcg gcggtataca gatgcgtctt ctccgccgca atctcaaact
      481 gctcgcggtc accgctccgc gccccgatga tcacaacatc ctcgaccgtc tcgaggacgc
      541 gccgggtgac gcaaccatgg ctgtagggcg ttccatcaag ttcgtcccgg agatccaggt
      601 gggcatcgca gaccacgtag acctccggct ccaccgccct gaccgccccg accgtcacgg
      661 tatgctcccc cccaagcatc accggcacct ttccgtcgcg gacgataccc cggacgaaat
      721 cggcaacctc atcaacaacg gcctccggca gcctggagac catgagatcg ccgagatccg
      781 tcaccgggac gtcaaagagt tcaatcccgg cagcggggtc gtaggcctcg aagttgaacg
      841 agaactccct gatagcccgc ggcccaaacc gcgtccccgg gcgaaaagac gtcgtcccat
      901 cgtatggaac accgaatatc gcgtagcggg cgtcctcata cggtgtatcc gcatccgcaa
      961 aatggagatg gacagggtcc tgcataaccg ttatatccag aagatctcac tcgagtttct
     1021 tcttgcccag agacgctatg taggcgactt ctttgcccgg ttcaagaccg gcggcctctt
     1081 ccgcggtcac gttgagttca aacatctcga agtccttgac gtccatgagc tggacggtcg
     1141 tgccggcgat cgagatcacc tgcgctatct tccgctcgac taccgggacg tatatctttg
     1201 ccgagaccgg ctgaacgatc gagcgcttga caccatcaaa gacccctata cagtctatac
     1261 gggactttgc cgctccgtgc ttccccggct tggagatggc gatggagaca atcctgcagg
     1321 gttcgtcatc cacgacaacg tagcgcccct ccttaagctt cccaacttct gtctgttcct
     1381 tcatatcgtc tcactgtggt aaacgcgtga ttaatgtatc tggattgcat tacataaata
     1441 caccgcagaa gaatagagaa ggacggggag aattgaggcg gttcatcagg gcgtgtgacg
     1501 atctggcagc ggccgtccgg gatgccgttg ccgatatggt cgggacgccg ggggcggggg
     1561 attgcgtcgg gatgggtgcg gacggcacac ccacaaagaa gatcgaccag gttgcagagg
     1621 atatcgtcgt cgactacctg gccgatcacc ccctctgcca gcggctgatc agcgaagaac
     1681 tcggctgcgc agcgatgggc gggaacaggg gcaccatctt cctcgacccc gttgacggca
     1741 catacaacgc cgtcgtcggg atcccgttct atgccatctc cattgcgtat gccgctgatg
     1801 ggatggttac cgcgggatac gtccagaacc tcgccaccgg cgagacgttc cacgccatcc
     1861 gcggggaggg cgccttccgg aacggccgcc cgatccatgt atcgaacgta tcgagacttg
     1921 aggagagcgc catgagcgtt tacggcagca ggatgcccga ccctgccccc atccagcgga
     1981 tcgaccggac gatcaggcgc tgccgtctcc ttggtgcatc ggcgcttgaa ctatgctacg
     2041 tcggttgcgg gcgcatcgag ggttttgtcg acctccgcgg aacgctccgg gtcacggacg
     2101 ccgcggccgg gatgctgata tgcaaggagg ccggcggtat ggtctccgac cccggaggcg
     2161 gcgaggttgt cttccccgac gacgtctccg ccggtcggcg cctcgtcgcc acgaacggca
     2221 tcctgcacag caggctcatc gaatatctga agggtaaccg cacatgagga taacactggt
     2281 atcgcgtctc gatgacgcgg aggcgctccg ctacaccgcc tcgcttggcc gggaactcaa
     2341 agacctcggg cacgacgtgg ccctggagga gggcacggca cgccatctca ggaggcatga
     2401 gggcaccccg tttgaggaga tcgacgccga cctggtcgtg gtcgtcggcg gcgacggttc
     2461 ggtgctccta accgtccagc gtatgagaaa acaggtcccc atactcggga tcaactgggg
     2521 ggaggtgggg ttcctcactg acctggagcc tggagaggcg attgatttct ttgccggcct
     2581 ggacgcgttt gaggtcgagc agcggatgcg ggtccgcctc tccgccggcg ggagtgtgct
     2641 cggcgatgcc ctgaatgagg ggctcatcgt caccgaccgc ccggcaaaga tgctccggtt
     2701 cggcgtctac gtcgacggaa cgctctcaga acggttccgg gctgatgggc tgctggtatc
     2761 gaccccgacc ggatcgaccg catacgccat gagtgcgggc gggccgatcg tcgacccgca
     2821 gatcgagggg atcctcctcg tcccgctcgc gccgtacatg ctctcgtccc ggccacacat
     2881 catcagcacc aggaggcgcc ttgagataac cctggagacc gataaaccgg cacacctcgt
     2941 catcgacggt cagaccacgc ttgaactccc gaaagaagca accctcacgg tcaagaagtc
     3001 ggatcaaccc gctttattcg tcaacaccgg caaacagttc tttgagaagg tgaaccggaa
     3061 actgcgtaac ctctgatccg gccggtgcct caatgagaga ttttatcggg ggccatgggg
     3121 attatcctca aaaacaggag tgagcacgat tatggaagac cagatgcgca cgaagatccc
     3181 gtatgcggag atcgcagaga tgctgaagag gagcctcaac ctccggggct cccccgtcgc
     3241 ggtgaaactc gccaggaaac ctgaagatat ccccgatggg gtggggccaa tcgaggagac
     3301 cgtccgccac tgccagatgg tcagcagggc acggctgaag ggcgagatct tctacgcgac
     3361 caccgagagg cacgcctgca tgggcggcgc ctgggctctc gggctccgcg aaatcacaga
     3421 gagcctcagc acaggtgagt tctactacaa actcgggaag ttcgagacct gggccgcctg
     3481 catgcgaacc atacagcagg tcccccacct cccggagccc acgacctacg cgaccgtata
     3541 cgcaccgctt gagaagacac cgtttgaccc ccatgtcgtt ctcatcatcg cagaaccgcg
     3601 ggcgatgctg aaacttgcgc aggcggaact ctacctcctc ggcggacgga tcgagtcgtc
     3661 catggccggg atccagtccg tctgcgccga tgcgacagcg ctgccatacc tctccggaaa
     3721 ggtcaactac tccctcggct gcgacggttc gcgccggttc tccgggatcg aggacaacga
     3781 acttgttatg gggatccccg ccgaactcct gccggagttc gcccgggcac tcccaatagt
     3841 caccggtgcg ccgggctcgg tgaagtgacg gggagaaaga ttc
//
LOCUS       DAOSZX010000118         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_19475_length_3789_cov_19.370380, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000118 DAOSZX010000000
VERSION     DAOSZX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19475_length_3789_cov_19.370380"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1350)
                     /locus_tag="PK196_07690"
     CDS             complement(22..1350)
                     /locus_tag="PK196_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HON41336.1"
                     /translation="MDAEEIARRHLADGIPDDEIVRLLSREVISIKRNRVTPEYAEAF
                     ARAVVTEVRNSTGLSGDLFTFEPSGSTMGEFGVGSRGAGDFFAHRQIARIIGRSDAAV
                     GVEEMDDAGAVSAGGDYIVVTVDGMHSRLSDFPFLAGFHVTRATLRDVYVMGAKPVAL
                     LSDIHVADDGDVAKVFDYTAGVTAVGEAMGVPLVTGSTLRIGGDMVLGSRMTGCVGAV
                     GVTSHLTARREIAPGDVLLMTEGAGGGTIATAAIYSGFPEVVEETINLHFLRACEALI
                     GSDALAGVHAMTDVTNGGLRGDAYEMAETAGCRIVVVEDELRSLVRPKVLAMLDALEI
                     DYLGVSLDSLLVVAPPENAAEIRRAVESAGVAMKEIGYVEEGAAESVLMVNGRIRDFS
                     PKFRESAYTPVKKVVDAEKRDFEEMKAGVRRAADAAIAKKERMLARLRSS"
     gene            1431..2231
                     /gene="thiD"
                     /locus_tag="PK196_07695"
     CDS             1431..2231
                     /gene="thiD"
                     /locus_tag="PK196_07695"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HON41337.1"
                     /translation="MTAGRMVAACSIAGSDSGGGAGIQADLKTFVELGVFGLTVVTAV
                     TAQNTREVRAAWPLPPEAVRTQIETVAEGFAIGAWKTGMLGNAATVRAVAESLPEGAI
                     SVIDPVMVSTSGHRLLEEDAISDLVEVLMPRASVVTPNIPEAEVLAGMRIEGVEGMAA
                     AGWRILDLGAGAVVVKGGHLPGGEAVDILVEREGEMKVSGERYPYAVHGSGCCFSAAL
                     TACLARGMALRESFCAAREFIDTAIREAVGDAGGVRVVNPGGRFIHRR"
     gene            2429..2860
                     /locus_tag="PK196_07700"
     CDS             2429..2860
                     /locus_tag="PK196_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HON41338.1"
                     /translation="MDEIDDAILRELQKDGRKSMAELGSMLGIAPSTVFKRIEKLKKA
                     GIIERFTIVINQKCFENTLIVFLNIRVQPEAKSDVEEFLREIPDVLDLYEVLEPGDFF
                     ARVRVQNISELKRDILVPLSSHPGVRDIQTIISVRRIKEQT"
     gene            2857..3144
                     /locus_tag="PK196_07705"
     CDS             2857..3144
                     /locus_tag="PK196_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41339.1"
                     /translation="MNISQVEMVQQMFAIVTLLLGVLLIGIIYNAYRIVKQPTLLLFI
                     YGLFTLVVGITFADLVSIFTLDEFIVAWSMVFSHVVVIIGLCVMAYGILRG"
     gene            3146..3598
                     /locus_tag="PK196_07710"
     CDS             3146..3598
                     /locus_tag="PK196_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41340.1"
                     /translation="MRTGTSFARDWQLIKVARSLREHDVTGSLLRRLLKDAPPGLMER
                     VAAIAERLGEEDGTALLSHAEERFDPPTLMEGLLLTWGVPSESTETAEGGVLLTIDCT
                     AAAVRDTFADARVAGPYLAGYARALQPDAVFERGGHGRVTIRFPPRER"
BASE COUNT          713 a         1067 c         1294 g          715 t
ORIGIN      
        1 gtccaccaga tggtatcctc ctcacgaaga ccggagccgg gcaagcatcc gctccttctt
       61 tgctatcgct gcatccgccg cacgccggac accggccttc atctcctcaa aatccctctt
      121 ctcagcatcc accacttttt tcacaggggt gtaggccgac tccctgaact tcggggagaa
      181 gtcccgtatc ctgccgttga ccatgagaac cgattcagcg gctccttcct cgacgtagcc
      241 gatctctttc atggcaaccc ccgcggactc cacggcacgc cggatctccg ccgcgttctc
      301 cggcggcgcg acaaccagga gggagtcaag ggagacgccc aggtaatcga tctcgagggc
      361 atcgagcatg gcaaggactt ttggccggac aaggctgcgg agttcgtcct cgacgacgac
      421 gatccggcag ccggcggtct cggccatctc gtaggcgtcg ccccggagcc cgccgttcgt
      481 gacgtcggtc atggcatgga caccggcgag ggcgtcgctt ccaatcagag cctcgcatgc
      541 cctgaggaag tggaggttga tcgtctcctc gacgacctcc gggaacccgg agtagatagc
      601 ggcggtggcg atcgtcccgc cgccggcgcc ctcggtcatc aggaggacgt cgccgggggc
      661 gatctccctg cgggcggtga ggtggctggt gacaccgacg gcgccgacgc agccggtcat
      721 ccgggaacca aggaccatgt ccccgccgat acgcaaggtt gaaccggtga ccagggggac
      781 gcccatcgcc tcgcctacag cggtgacgcc ggcggtgtag tcgaatacct tggccacatc
      841 gccgtcatcg gcgacgtgga tgtcggagag gagggcgacg ggttttgcgc ccatgacata
      901 gacgtcacgc agcgtcgccc gggtgacgtg gaacccggca aggaatggga agtctgagag
      961 ccgggagtgc atcccgtcga cggtgacgac gatgtaatcg ccgccggcgc tcaccgcccc
     1021 ggcatcgtcc atctcctcga ccccgacggc ggcatcggat cggccgatga tccgtgcaat
     1081 ctgcctgtgg gcgaagaagt ccccggctcc gcgtgaaccg acaccgaact cgcccatcgt
     1141 cgacccggag ggctcgaagg tgaagaggtc gccggagagg ccggtcgagt tccggacctc
     1201 tgtgacgacc gccctggcga acgcttcggc gtattcgggg gtgacgcggt ttcgcttgat
     1261 cgatatgacc tcccgggaga ggaggcgcac gatctcgtca tcagggatgc cgtcggcgag
     1321 gtgccggcgg gcgatctctt ctgcatccat tgcagatctg gtcggttcgg atacgagata
     1381 aatgagatgg cgggcaaaga ttcaagcgtg taccgggcga caccagaggt atgacggctg
     1441 gaaggatggt cgccgcctgc agcatcgccg gttccgactc ggggggcggg gcgggtatcc
     1501 aggcggactt gaagacgttt gtggagctcg gggtcttcgg gctgacggtc gtcacggccg
     1561 tgaccgccca gaacacgaga gaggtgcggg cggcctggcc gctcccgccg gaggctgttc
     1621 gaacgcagat tgagacggtc gctgaggggt ttgcaatcgg cgcctggaag accgggatgc
     1681 tcgggaacgc cgcaaccgtc cgggcggtcg cggagagcct gccggagggg gcaatctctg
     1741 tgatagaccc ggtgatggtc tcgacatcgg gccacaggct gctcgaggag gatgctatca
     1801 gcgacctggt ggaggtcctg atgccgcgtg caagcgtcgt caccccgaac atcccggagg
     1861 cggaggttct tgccgggatg cggatcgagg gggtggaggg tatggccgcg gcaggctggc
     1921 ggatcctgga tctcggcgcc ggggcggtgg tggtgaaggg ggggcacctc cccggcggtg
     1981 aggcggtcga tatactggtc gagcgtgagg gggagatgaa ggtctcgggt gagcgctacc
     2041 cctacgcggt gcacggctcg gggtgctgtt tctccgcggc gctgacggca tgcctggcga
     2101 gggggatggc tctgcgggag agtttctgcg cggcacggga gttcatagat acggcaatcc
     2161 gggaggcggt gggcgacgcc ggcggggtca gggtggtcaa tcccggtggg aggtttatcc
     2221 accgtcgatg aaaatctttt cccagatttt cgctgaaaca gagaatcgtt tccataatac
     2281 ccacggaaaa ccggtctctt cccggcgggg agaatcggtg ggaaaacgcg gtgaagtctt
     2341 gataatccat caaaaatgcg gtataaactc tttatccctc ccggacaaaa cacaatttta
     2401 atattcaaaa acattcaacc agtctacaat ggatgagatc gacgatgcga ttcttcgcga
     2461 gctccagaaa gacgggagga agtccatggc agaacttggc tcaatgcttg gaatcgcacc
     2521 gtcgacggtc tttaaaagga tcgagaaact taagaaagcc ggcatcatcg agcggttcac
     2581 gatagtcatc aaccagaagt gctttgagaa tacactgata gtctttctaa acatcagggt
     2641 ccagccggaa gcgaagtcgg acgttgagga gttcctgcgg gagatcccgg acgtccttga
     2701 cctctacgag gtgcttgagc ccggcgactt cttcgccagg gtgcgggtgc agaacatatc
     2761 cgagttaaag agggatatcc tggtcccgct ctccagccat ccgggggtgc gggatatcca
     2821 gactataatc tcggtgagaa ggataaagga gcagacatga acatatcgca ggttgagatg
     2881 gttcagcaga tgttcgcgat cgtgacactc cttcttggag tgctgcttat cggcatcatc
     2941 tacaacgcat acaggatcgt gaagcagcct accctgctgc tcttcatcta cgggcttttt
     3001 acgctggtgg tggggatcac cttcgccgat cttgtgagca tatttacgct tgatgagttc
     3061 atcgtggcct ggtcgatggt cttttcccac gtggttgtga tcatcggtct ctgcgtgatg
     3121 gcgtacggca tactgagagg gtaagatgcg gaccggtaca tcgtttgccc gggactggca
     3181 gcttatcaag gtagcgcggt cgctgaggga acacgacgtt accgggtcac tgctgcggcg
     3241 gctgctgaag gacgcccctc ccgggcttat ggagcgggtc gccgccatcg cggagaggct
     3301 gggtgaggag gacgggacgg cgcttctctc tcacgccgag gagcggttcg atccgccgac
     3361 cctgatggag gggctcctcc tcacctgggg cgtaccctct gagtcgacgg agaccgcgga
     3421 ggggggcgtt ctcctcacca tcgactgtac ggcggcggcg gtgagggata cctttgcgga
     3481 cgcacgggtt gccgggccgt acctggccgg gtatgcgagg gcgctccagc ccgacgcggt
     3541 attcgagcgc gggggccacg ggagggtgac gatccggttc ccgccgcggg agaggtgagg
     3601 aggtttatat cgagagaccg gctcttcgag acggctggag tttttttgca taatccgggc
     3661 caaaggcggc ccggagggta tgaccggctg aaataggagt cctgccggta tccagaccgg
     3721 cggcgcgcct ttcggggtgg gcgtgaggat cggccggggt ttcaccggga gagaacattc
     3781 cagaggtgg
//
LOCUS       DAOSZX010000119         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_19827_length_3733_cov_19.707993, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000119 DAOSZX010000000
VERSION     DAOSZX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19827_length_3733_cov_19.707993"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1290
                     /locus_tag="PK196_07715"
     CDS             73..1290
                     /locus_tag="PK196_07715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON41341.1"
                     /translation="MRDQPDYDVIVAGGGPAGSTAAFLLAKAGHRVGLFERVVYPREK
                     VCGGCLTQKSVRFLDRVFSLPLDALQREGLLDFTGKGYALYIGNRRVLAEGLAEPFYF
                     TRRDRYDACLARMAREAGAEVREGTEVTAVDHVQRTVTTADGERCSARVIVGADGIHS
                     SVRRSLPEGVVDRQRWRRNLGLALEIKINRGEVGMHQNREAAIPLDADMGIPHVFLGA
                     CPMGYGWVFPNTDAVIVGIGGLFEANRRGLPGRFREFLGSIGFAAFADRKPAGCLLPF
                     GNYIPSPAHEATVLVGDAGGFTSPILGEGIFYAQRTAELAAHAIDRHLTSGSPLAATY
                     TSLLNRRLIPELEAEMRIRDFLYSHLDGRTPVILLRLLIRATRRRVIDAVQGHRSFQG
                     LFRRDEDLHTAVW"
     gene            complement(1310..1747)
                     /locus_tag="PK196_07720"
     CDS             complement(1310..1747)
                     /locus_tag="PK196_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HON41342.1"
                     /translation="MIVRYTLQVSLDAFDRSGRPLCGDRPDQNGFCRLCLRCPFAVRD
                     DARVYCERFETPIRAREKYALQGWKKTRRRVLERDRERCAICGTGQDLHIHHIDRDPS
                     NDDLANLMTLCAICHARVHAGLRHEDGEERVIRVLQAAMKRNR"
     gene            complement(1843..2931)
                     /gene="dinB"
                     /locus_tag="PK196_07725"
     CDS             complement(1843..2931)
                     /gene="dinB"
                     /locus_tag="PK196_07725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HON41343.1"
                     /translation="MTTTGNRIVLHADMDSFFASIEILRDPSLSGRPVIVGADPRGGA
                     GRGVVSTCSYEARRYGVHSGMPISRAYDLCPHGIYLPVDRPLYLQVSERVMAILSRHS
                     RLFEQVSIDEAYLDLSDIGDFTAAEASAASIKQEIREETGLTCSVGVAPGKTVAKIAS
                     DYRKPDGLTIVRPEEVAEFLAPLPVEKIPGIGRRSGEELQRMGIVTIGDLAHRDIQEI
                     VAHLGRPGIRVRRLAGGIDGAEVRSREGRKSISRERTFDEDTSDPTVIPETLAGLAES
                     VVEMLRSAGLRCRTVTVKVRYRGFQTYTRSKTLERFTADPGVIQRAVSELLQPFLNGT
                     DVRLVGIRLSNLEGGGRTRQASIEEFCA"
BASE COUNT          714 a         1147 c         1154 g          718 t
ORIGIN      
        1 atccaggcag acggtccggc ggggaaacaa tatccggttt caaggcaaac ctttaacaaa
       61 agcgaaagtc gaatgcggga ccagccggat tacgatgtta tagttgccgg aggaggaccc
      121 gccgggagca ccgccgcctt cctgcttgca aaggcggggc accgcgtggg gctctttgag
      181 cgggtggtat acccacgtga gaaggtctgc ggcggttgcc tgacacagaa gtctgtccgg
      241 ttcctggaca gggtcttttc cctcccgctc gatgcactcc agcgggaggg gctgctcgac
      301 ttcaccggga aggggtatgc cctctacatc ggtaatcgcc gcgtcctcgc cgaaggcctg
      361 gcagaaccgt tctacttcac ccggcgggac agatacgatg cctgccttgc ccggatggcg
      421 cgggaggccg gggcggaggt ccgggagggg actgaggtga ccgcggtcga ccacgttcag
      481 cggacagtaa ccaccgcgga cggggagcgt tgctccgcca gggtcatcgt tggggctgac
      541 gggatccaca gcagcgtccg ccgctctctc ccggagggtg ttgttgatcg gcaacggtgg
      601 cgcaggaacc ttggcctggc gctggagata aaaatcaacc gcggggaggt ggggatgcac
      661 cagaacaggg aagcggcgat ccctctggac gctgacatgg ggatacctca cgttttcctc
      721 ggcgcctgcc ccatgggtta cggctgggtc ttccccaaca ctgatgcggt catcgtgggt
      781 atcggtggtt tgtttgaggc aaatcgaagg gggcttcccg gccggttcag ggagttcctg
      841 gggagtatcg ggtttgcggc gtttgcagac cgaaagccag ccggatgcct tctgccgttt
      901 ggaaactaca tcccatcccc ggcccatgag gctacggttc tggtcgggga tgcaggcggt
      961 ttcacaagcc ccatccttgg ggaggggatc ttttatgccc agagaacggc agaactcgcc
     1021 gctcacgcaa tcgaccgcca cctcacctcg gggtcgccgc ttgccgcgac atacacctcc
     1081 ctcctgaacc gtcgtctcat ccctgaactg gaggcggaga tgaggatccg ggacttcctc
     1141 tacagccacc tcgacggcag gacgcctgtc atcctgctcc gtctcttgat cagggcaacg
     1201 cgtcgccgtg tcatcgatgc tgtccagggg caccgttctt ttcaggggtt gttccggcgt
     1261 gatgaggatc tccacacagc agtctggtga gaacacccga cgggttttct cagcggtttc
     1321 gtttcatcgc ggcctgcaga acccttatta ccctctcttc tccatcctcg tgccggagac
     1381 cggcgtgcac cctggcatga cagatcgcgc agagcgtcat gaggtttgcc aggtcatcgt
     1441 ttgaagggtc gcgatcgatg tggtggatgt ggaggtcctg ccctgtgccg cagatggcac
     1501 agcgctctct gtcacgttca agaaccctgc ggcgtgtctt cttccacccc tgcagggcat
     1561 acttctcccg tgcccggata ggggtctcga accgctcgca gtagacccgg gcgtcgtctc
     1621 tcacggcaaa agggcaccgg agacagagcc ggcaaaagcc gttctggtcg gggcggtcgc
     1681 cgcagagagg gcgtcctgaa cggtcaaagg cgtcaaggct cacctgaaga gtatatctca
     1741 cgatcatgag ggtttgtgtg cggcgtgtga taaaacccgg tcccgttccc tcaaccctga
     1801 aaccggccgg ggcgatggtg gaaatcttgc tctttcttag cctcaggcgc agaactcttc
     1861 gatcgaggcc tggcgggtgc gcccgccgcc ctccaggttt gatagccgga tcccgacgag
     1921 ccggacgtca gtgccgttca ggaacggctg gagaagttca gagaccgccc tctggatcac
     1981 gcccggatcc gcggtgaaac gctccagagt ctttgaacgg gtatacgtct gaaaccctct
     2041 gtacctcacc ttgactgtga ccgtacggca ccggagaccc gcggagcgaa gcatctccac
     2101 aacactctcc gcaagcccgg ccagggtctc agggatcacc gtcggatcgg aggtatcctc
     2161 atcaaaagtc ctctcccggg atatggactt tcgcccctcc ctggaccgca cctccgcgcc
     2221 gtcgataccc ccggcgaggc ggcgcacccg gatgcccggc cgtccgaggt gcgctactat
     2281 ctcctggata tcccggtgtg caagatcgcc gatggtcacg atgcccatcc gctggagttc
     2341 ctccccgctc cgtctcccga tccccgggat cttctccacg gggagggggg ccaggaactc
     2401 cgcgacctct tccgggcgga cgatcgtcag gccgtctggt ttacggtaat cggaagctat
     2461 ctttgccacg gtctttcccg gggcgacccc caccgagcag gtaagcccgg tctcttcgcg
     2521 gatctcctgc ttgattgagg cggcactcgc ttctgccgct gtgaaatcgc cgatatcgct
     2581 gagatcgagg taggcctcgt ctatgctcac ctgctcaaac agcctgctgt gccgggagag
     2641 gatcgccatg accctctctg agacctggag atagagcggc cgatccaccg ggaggtagat
     2701 gccgtggggg cagaggtcgt aagccctgga gatgggcatc ccggaatgca ccccgtatcg
     2761 cctggcctca tacgagcacg tgctcacgac cccccggccg gcccctcccc tcgggtcggc
     2821 gcccacgatc accggccgcc cggaaagcga tgggtcgcgg aggatctcga tggaggcaaa
     2881 gaaactgtcc atgtcggcgt gcagcacgat cctgttgccg gtggttgtca ctcgatgaac
     2941 ctcttttccg gtctcttgca ggagtattgg aggctcggtg atataggagg tttggtggtg
     3001 ctgtgggaga gatagtgcaa gactggaaaa ggatttcacg ggtgccggat atgagccagg
     3061 aacggcttcc tacgatcata tcggcaatga ccatgtgaaa gcccgaaaac ggcttcccac
     3121 aacagctgga cacgttagac catgaaaaat gccccctaca gtggaccgga ggggatatcg
     3181 ccacgcactg cccctgcccc tagggggcgg gggaggaggc cggaggccgg gcggggtggg
     3241 ggtcacaggt atagccttac ggggtagaga caggaaggtg gaatactccc gacccgccag
     3301 gcccagatct gctctcgcaa cgggcccata agtgatggag acagggggga cccccctccc
     3361 cgtcagcccc acccccaaag gcgatatccc ctcggaagcc gttttcgaga tttaattttc
     3421 aacccgcatc cccacccacc aactcaggca cactttcgcc gggtatttct gcaacgcaga
     3481 tctgacatat ctacccaccg gaaccacgcg cctcaaccca acccccgcgc gcctcccggt
     3541 gaccggggcg ggcatcccgg cggtcgggga ggcaggaagt atgaataaaa taccgaaaat
     3601 cggcagactt cgtgctatga atttacacca tcataatacg tgggcgagtg agaaccactc
     3661 gctccaccaa ctccgtctga tcggcaggaa tgtttgtgat cgcggggtta tgaggacgtg
     3721 caaataaaaa gtg
//
LOCUS       DAOSZX010000120         3726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_19880_length_3726_cov_17.518932, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000120 DAOSZX010000000
VERSION     DAOSZX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19880_length_3726_cov_17.518932"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK196_07730"
     CDS             complement(<1..545)
                     /locus_tag="PK196_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HON41344.1"
                     /translation="MRSIRLIPPVSFLILLLLLAATAGAVPLTPGVPPVAMETADGGR
                     NVTLTDDLGETVVIEGTPQRIISLAPSNTEILYALGLEDRVVAVTDYCDYPPETVDKP
                     TVGGFSTVNIEKVIAMNPDLILAAPANTEEIIDRLRSLGLTVVTLDPQAIEDVLHEIE
                     LLGRVTGNEAEASALVGELRAR"
     gene            complement(615..1871)
                     /locus_tag="PK196_07735"
     CDS             complement(615..1871)
                     /locus_tag="PK196_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON41345.1"
                     /translation="MKPIEIIGVDVSYGAKKILEEISFHADKGEILGIIGPNGSGKTT
                     LLRAMSRVVARDRGEILLDSHDLDTLGHRELARRVAVVPQDISIGFDYTVRDIVMMGR
                     HPHIGRLASETVRDVEICDRAMRLANVAHLAGASVHDISGGERQRVLIARALAQEPEI
                     LLLDEATSNLDVSYQVEILNIIRGLTGRITVVSVFHDLNLAAYYCDRLLLLKDRKVYA
                     IGAPADVLTCENIREIFGMETLVRPHPLTGRPYVLPVYMRTAGAGVNRRVHLVCGGGT
                     GSDILYLLRAAGFTVTCGVLNVLDTDYGTAMHLGIPAVAEPPFHGITTASLAAVREWL
                     DRADAIVVTAMPIGPGNLDNIRVLLDYPEKTIILYSGSSTARMEDCTGGEADKVLAEI
                     EARGAVRVEGAEDLIALLSQQQIGRE"
     assembly_gap    1889..1988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1989..2653)
                     /locus_tag="PK196_07740"
     CDS             complement(<1989..2653)
                     /locus_tag="PK196_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HON41346.1"
                     /translation="MRRTAPLTILALVCALIISVGCAVTIGPAGIPLKTLLSSPNTWM
                     IFWDVRLPRVFTAALVGCGLAVAGTTMQGLFRNPMADPYIIGTSSGGALGATIAIVLL
                     AGNGRPVLAFLGAIAATFTVYFIARSGGKIPVETLLLSGVALSTLLSALLSFLMYTAG
                     RSLHQIMFWLMGGFWNVSWNDFFISLPILMGCGGIYLFARDLNVLSLNEEDAIHLGVN
                     VEQV"
     gene            2774..>3726
                     /locus_tag="PK196_07745"
     CDS             2774..>3726
                     /locus_tag="PK196_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HON41347.1"
                     /translation="MGNLNVAVLGPAGYAKDLGKKGTESDIIFYNLKKGGDTVTFIEP
                     ARYPERLAPLFYAASMADEALVVVSEITPIFGEWVLMLDEVGVERGSIILKNYLTPPD
                     LAPLLRGTVLEGYEFVDDDPIALRERLIARAHARRPVPPEPGATGTVTIDHHFNIRGI
                     GTVILGGVVRGGIRKHDTMTVYPGERAVTVRSIQKHDDDFDWAAEGDRVGLALKNIEA
                     DDLDRGYVLSTDPAIRVGTGLKARATLVRYWPVPIARGMVLHLGHWMQFIPARVEAVQ
                     DDGDWRRPMLTLALEKDLVCLPGDRAVLHYLEGGKLRIAGH"
BASE COUNT          745 a         1087 c         1112 g          682 t
ORIGIN      
        1 cgcgcccgaa gttcgccgac aagcgccgag gcctccgcct cattgccggt aacccttccg
       61 agaagttcga tctcatgcag aacgtcctcg atcgcctggg gatcgagggt gacaacggtc
      121 agcccaagcg agcgcagccg gtcgatgatc tcctccgtat tggcgggcgc ggcaaggatg
      181 agatccgggt tcatcgcgat caccttctcg atgttgacgg tgctgaaccc gccaaccgtt
      241 ggtttatcga ccgtttccgg aggatagtca cagtagtcgg tgacggcgac cacccggtcc
      301 tcaagcccga gggcatacag tatctcggtg ttggagggcg caagcgatat gatcctctgc
      361 ggggttccct ctatgacaac ggtctccccg aggtcgtcgg tcagcgttac gtttcggccg
      421 ccatcggccg tttccatcgc tactggtggc acgccggggg tcagaggcac agcacccgct
      481 gtagcggcga gaagcaagag cagtatgaga aacgatacag gaggtatcag gcgtatggat
      541 cgcataacca tgaatagacc ccattgagcg taataatttc caggacccgg cgacccaatg
      601 cagcgggtgc ggggttactc ccggccgatc tgctgctgcg agaggagggc gatgagatcc
      661 tccgctccct cgaccctcac cgccccccgc gcctcaatct ccgcaagaac cttatccgcc
      721 tcacccccgg tgcagtcctc cattcgggcg gtgctgctcc ccgagtagag gatgatggtc
      781 ttttcagggt aatcgagcag cacacgaatg ttgtcgaggt tcccgggacc tataggcatt
      841 gccgtcacga cgatggcatc cgcccggtca agccactccc tgacggccgc aagggatgcc
      901 gtggttatgc cgtgaaacgg gggctctgca acggcgggga taccaaggtg catggccgtt
      961 ccgtaatccg tatccaggac gttcaggacg ccgcaggtga ccgtgaaccc cgcagcacgg
     1021 agcaggtaaa gtatatcaga ccccgtcccc ccgccgcaga cgagatgcac ccgccggttc
     1081 accccggcac cagcggttcg catgtagacc ggcaggacat agggtctccc tgtaaggggg
     1141 tgtggtctga caagcgtctc catcccgaag atctcgcgga tgttttcgca tgtgaggaca
     1201 tccgcaggcg ccccgatcgc gtagaccttc cggtctttga ggagcagcag tcgatcacag
     1261 tagtatgcag ccaggttgag gtcgtggaag acgctgacca ccgtgatcct cccggtaagg
     1321 cctctgatga tgttgaggat ctcgacctgg taactgacat ccaggttcga tgtcgcctcg
     1381 tcgagcagga ggatctctgg ctcctgtgcg agcgcccggg cgatgagcac cctctggcgc
     1441 tccccgccgc tgatatcgtg caccgatgcg ccggcaaggt gcgcaacgtt tgcaaggcgc
     1501 atggcgcgat cacagatctc cacgtcccgc accgtctcgg aggcaagcct ccctatgtgg
     1561 gggtgccttc ccatcatgac gatatcacgc accgtgtagt cgaaaccaat cgatatgtcc
     1621 tgtggaacga ccgcaacccg gcgggcaagt tcacggtgcc ccagggtgtc caggtcgtgg
     1681 ctgtcaagaa gaatctcccc acggtccctg gcaacaaccc ggctcatcgc cctgagcagt
     1741 gtcgtcttcc ccgagccgtt cggtccgatg atccccagga tctcgccctt atccgcgtgg
     1801 aacgagatct cctccaggat cttctttgcc ccgtaggata catccacccc gatgatctcg
     1861 ataggtttca tgctttcatc cggctccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnac ctgctcgacg ttcaccccga gatggatcgc atcctcctcg tttaatgaga
     2041 ggacgtttag gtcgcgtgcg aagaggtaga tccctccaca ccccatcagg atggggagag
     2101 atatgaagaa gtcgttccag gatacattcc agaatccgcc catcagccag aacatgatct
     2161 ggtggaggct ccgccctgcg gtatacatca aaaaggagag gagggctgaa agaagcgttg
     2221 agagagcaac gccggagagg aggagcgtct cgaccgggat ctttcccccg ctccgggcga
     2281 tgaagtagac ggtgaaggtt gcggcgatcg cccccaggaa cgcgagtact ggacggccgt
     2341 tgccggcgag gaggacgatt gcaatcgtgg ccccgagcgc cccgccagag gacgtgccga
     2401 tgatgtaagg atctgccatg gggttcctga agagtccctg cattgtcgtg cccgccacgg
     2461 caagcccgca gccgaccagc gccgcggtga agaccctggg aagacggacg tcccagaaga
     2521 tcatccaggt gttcggtgaa gagaggaggg ttttcagggg gatgccggca ggcccgatag
     2581 tgacggcaca gccaacgctg atgataaggg cgcagacgag tgccaggatg gttagcggtg
     2641 ccgtcctccg catagatcag gtataaaatt aagtttactt tgttaaaatt atttggatta
     2701 aggcgcggca gatgcccgtc ccgaccggaa cggagcaaac ctcatacccc gcgagagcga
     2761 agagatctgg catatgggca acctgaatgt tgccgtgctc ggccccgcag gttatgcaaa
     2821 agacctcggg aagaagggca cggagtccga tatcatattt tataacctga agaaaggcgg
     2881 ggataccgtc accttcatcg agcctgcgcg ctacccggag cgactggctc cgctctttta
     2941 tgctgcgtcg atggctgatg aagccctcgt cgttgtgagc gagatcaccc cgatcttcgg
     3001 ggagtgggtg cttatgctcg atgaggttgg ggtggagcgt ggcagcataa tactcaaaaa
     3061 ctacctcacc cctccggatc ttgcgccgct gctccggggg acagtgctcg aaggctacga
     3121 gttcgtcgac gacgacccta tagcgcttcg cgaacggctg atcgccaggg cgcacgcccg
     3181 tagacccgtc ccgccagagc caggcgccac gggaacggta accatagatc accacttcaa
     3241 catccgcggc atagggacgg tcatcctcgg cggcgttgtg cggggcggta tcaggaaaca
     3301 cgataccatg acggtctatc ccggagaacg tgctgtcacg gtgcgctcaa tccagaaaca
     3361 cgatgacgac ttcgattggg ccgccgaggg tgaccgtgtg gggctcgcgc tcaagaacat
     3421 cgaggccgac gacctcgatc gcggctacgt cctatcaact gatcccgcca tccgcgtcgg
     3481 gacgggactc aaagcgcggg caaccctggt caggtactgg ccggtgccta ttgcacgcgg
     3541 catggtgctc catctcggcc actggatgca gttcataccg gcgcgggtcg aggcggtgca
     3601 ggacgacggg gactggcgaa gaccgatgct tacgcttgcg cttgagaaag accttgtctg
     3661 cctcccgggt gacagggcgg tgctccacta tctcgagggc gggaaactca ggatcgccgg
     3721 gcatat
//
LOCUS       DAOSZX010000121         3707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_20012_length_3707_cov_18.697152, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000121 DAOSZX010000000
VERSION     DAOSZX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20012_length_3707_cov_18.697152"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..82)
                     /locus_tag="PK196_07750"
     tRNA            complement(11..82)
                     /locus_tag="PK196_07750"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Ala,seq:ggc)
     gene            complement(141..1268)
                     /locus_tag="PK196_07755"
     CDS             complement(141..1268)
                     /locus_tag="PK196_07755"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HON41348.1"
                     /translation="MGWFEVVGEDAIRNGECTDVYFKRVVEVMERDGVNPQVTMEVTA
                     ASLPDPWAVFCGLDDVIKLLEGLPVDVDAMPEGTIFCPNEPVLRISGRYRDFAVYETA
                     ILGFLCHASGVATAAAHMKVAAAGRPVYSFGSRRQHPAIAAMIERAAWIGGVDGVSNT
                     AAPEGIPLAGTMPHAFVMCYPTPEDAWLAFAHSAGPDVPRVMLADTFSDEKDEAVRAA
                     ACGAEAVRLDTPRSRRGDLRAIVEEVRWELDIHGYPEVKIFLSGGLTRADVAAFHDVA
                     DAFGIGGAIANAPVIDFALDIVELKGRAFAKRGKRSGVKQVYELPGGHRLTLPAQTPA
                     PKDAVPLLVPSVRNGAPVVRPRMEDARERVLSRLRAPAGRG"
     gene            1421..2095
                     /locus_tag="PK196_07760"
     CDS             1421..2095
                     /locus_tag="PK196_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HON41349.1"
                     /translation="MLGIIGGTSLLFADLPALEKRDVATPYGKAEVHTGEEFALLMRH
                     QHNLPPHRINYRACLAALAVLGVDEIVAFGSSGSLKHEIEPGSIAIPTDYISVTEIPS
                     IYDFTIEHIRPELDADLVRTLGDLVPEARVGGVYAQTRGPRIETVAEVRALAGVADLV
                     GMTVASEATLALELGMRFAAICTVDNYANGLGGETLTYEHILAVSRANARRTERILEK
                     IVEQLA"
     gene            2092..3402
                     /locus_tag="PK196_07765"
     CDS             2092..3402
                     /locus_tag="PK196_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HON41350.1"
                     /translation="MNDTFSARGSILIAGVATIDGSTADIAIDETGTIVAIGDDARGA
                     IDADIIIDGSERLAIPGLVNTHTHAAMSLLRGYADDMILQDWLSQKIWPLEAHLSGDD
                     VYAGTRLACLEMIRSGTVAFNDMYFFMDRAAAAVDEMGLKATLAYGFIDLGMEDKREA
                     EIRATEALVRRIASLKNPRIRAAVGPHSVYTVSPEGLSWCAGFAKEQNIGIHVHLSET
                     GKEVTDCLAQYGKRPAHLLDDCGCLTPRTVAAHCCWLDESECRLLGQRGVHASHNPAS
                     NMKLAVNRAMPCHWLKQYGAGLTLGTDGCSSNNNLDLFEEMKFAALLQKFAWNSPTLL
                     PAGEVFTMATAGGARALGTGPGTLTVGAPADIALLDTRAVCNTPLFHAESNLVYACNG
                     GAVRTVLCQGRILMHEREVPGEEEIIREAAAAAHALVERAGDEG"
BASE COUNT          727 a         1195 c         1123 g          662 t
ORIGIN      
        1 caaaacaaga tggaccgagc gggatttgaa cccgcggcct ctaccatgcc aaggtagcga
       61 tctccccctg atctatcggc ccctgttacc ctataacgtt ggccccgatc acataataag
      121 ggttcgggca gattgccgcg ctatcctctc ccggcaggag ccctgagccg ggagagaacc
      181 cgttcccgcg catcctccat cctcgggcgc acgacgggcg caccgttcct gacagacggc
      241 acgaggagcg gcaccgcatc tttcggtgcc ggggtctggg ccgggagcgt caggcgatgg
      301 ccgccgggaa gttcgtagac ctgtttcacg ccgctccgct tcccccgctt tgcaaacgcc
      361 cggcccttga gttccacaat atccagggca aaatctatca ccggagcgtt tgcgatcgcg
      421 ccgccgatac cgaaggcgtc agcaacgtcg tggaaggctg cgacatccgc acgggtcagg
      481 ccgccggaaa ggaagatctt cacctccggg tatccgtgta tatccagttc ccagcgcacc
      541 tcctcaacta tcgccctcag atcaccccgg cgagaccgtg gcgtgtcaag ccggacagcc
      601 tccgccccgc aggccgccgc cctgacagcc tcatccttct catcagagaa ggtgtctgca
      661 agcatcaccc gcggcacatc agggcccgca ctatgtgcaa acgcaagcca agcgtcctcc
      721 ggcgtcgggt aacacatcac aaacgcgtgc ggcatcgtcc cggcaagggg gatcccctcc
      781 ggcgcagcgg tgttgctcac accgtccacc ccgccgatcc aggccgcccg ctcgatcatt
      841 gccgcgatcg ccgggtgctg ccggcgcgag ccaaacgagt ataccggccg ccccgccgca
      901 gccaccttca tgtgggcggc agccgtcgcc acccccgacg cgtggcagag aaaacccagg
      961 attgcggtct cgtagaccgc gaagtccctg tagcgcccgg agatccggag taccggctcg
     1021 ttcgggcaga agatcgtgcc ctccggcatc gcatccacgt ccaccgggag accctcgaga
     1081 agtttgatta catcatccag accgcagaag accgcccagg gatccgggag tgatgcagca
     1141 gtcacctcca tcgtgacctg cgggttgacg ccgtcccgct ccatgacctc gaccactcgc
     1201 ttgaagtaga catctgtgca ctcgccgttc ctgatcgcat cctcgccgac cacctcaaac
     1261 cagcccataa gtattccggt tgacgcaggg ggatgtaaat ctttaccgac cccggggcat
     1321 cgtatggtga gcctttttca gccgggaagt ccacccttgt agagagtatg tctgatggag
     1381 aaacgaatgc tgcaacgccg gccaacctgg agcggttctg atgctcggga taatcggggg
     1441 caccagcctg ctcttcgccg acctgccggc gcttgagaag agagacgtcg ccactcctta
     1501 cgggaaggcg gaggtgcaca ccggagaaga gtttgcgctg ctgatgcgcc accagcacaa
     1561 ccttccgccg caccggatca actaccgcgc ctgcctcgcc gctcttgcgg ttctgggggt
     1621 ggatgagatc gtcgcgttcg gttcatcagg gtcgctgaag cacgagatcg agcccggttc
     1681 gatcgccatc ccgacagact acatcagcgt caccgagatc ccgtccatat acgacttcac
     1741 catcgaacac atccggccgg aactcgacgc agacctcgtc cgcacccttg gcgacctggt
     1801 gccggaggcc agggtcggcg gggtctacgc ccagacccgc gggcccagga tagagaccgt
     1861 cgccgaggtg cgggctctcg caggtgtggc ggatctcgtc gggatgaccg ttgcaagtga
     1921 agcgacgctt gccctggaac tcgggatgcg gtttgccgcc atctgcaccg tggataacta
     1981 cgcaaacggt cttggtggag agaccctgac ctatgagcat atccttgcag tctccagggc
     2041 gaacgcccgg aggacagaga ggatccttga gaagattgtg gagcaacttg catgaacgat
     2101 accttttccg cacgtggttc gatcctgatc gccggggtcg ccaccatcga tggatcgacc
     2161 gctgatatag caatcgacga gaccggcacc atagtcgcga tcggggatga cgcaagaggc
     2221 gcgatcgacg ccgatatcat aatcgacggt tctgagaggc tcgcaatacc cggactggtc
     2281 aacacccaca cccacgccgc catgagtctg ctgcgagggt atgcggacga tatgatactc
     2341 caggactggc tctcgcagaa gatctggccg ctcgaggccc acctctccgg cgacgacgta
     2401 tacgcgggga cgagactcgc ctgcctggag atgatcagga gcggcaccgt cgcgttcaac
     2461 gacatgtact tcttcatgga ccgggccgca gcggcggtcg atgagatggg gctgaaggca
     2521 acgctcgcct acgggttcat cgatctcggt atggaggata aacgcgaggc cgagatcagg
     2581 gcgacggagg cacttgtccg ccgcatcgcc tcgctcaaaa acccccgtat ccgggcggcc
     2641 gtcggcccgc actccgtata caccgtctcc cctgagggtc tctcctggtg tgccgggttt
     2701 gcaaaagagc agaatatcgg tatccacgtc cacctctccg agaccgggaa ggaggtcacc
     2761 gactgcctgg cgcagtatgg aaagcgcccg gcgcatctcc tcgacgactg cggttgtctc
     2821 accccccgca ccgtcgccgc gcactgctgc tggcttgacg agtctgagtg ccgtctcctc
     2881 gggcagcgcg gcgtccacgc ctcccacaac ccggcaagca acatgaaact tgccgtaaac
     2941 cgggcgatgc cgtgccactg gcttaagcag tatggggcgg gtctcaccct ggggacagac
     3001 ggctgctcct caaacaacaa cctggatcta tttgaggaga tgaagtttgc agcactgctg
     3061 cagaagttcg cctggaactc cccgaccctc ctccccgcag gcgaggtctt cacgatggcg
     3121 accgcagggg gcgcccgtgc gcttggcacc ggcccgggga cgcttacagt cggcgctccg
     3181 gccgatatcg ccctcctgga tacacgggca gtctgcaaca caccgctatt ccacgccgaa
     3241 tcaaacctcg tctatgcctg caacggcgga gcggtcagga cagtcctctg ccaggggagg
     3301 atcctgatgc acgagcggga ggtgccggga gaggaggaga tcatccggga ggccgctgct
     3361 gcagcccatg cgcttgttga acgggcgggg gatgaggggt aggtctcagg gttttgcagg
     3421 atctgccgcc atctgctctc aaggttcgag gtgccgacct gtgggaggtg ggagatgagg
     3481 tggaggattc aaattgtgca gagtgagcgg gatctgcccg gccgctgaga gttgtcatca
     3541 aaccagtacg gctatcggtt tgatggcctc gtgtagacgc gtgtgacaga agaggttgaa
     3601 cgttgactta tcatgcgtgg atatcggtat cccgcagtgt gttggtccct ccacctcctg
     3661 gttgacggcc ggatgataga gcccccgcat gccctgaggg cgattaa
//
LOCUS       DAOSZX010000122         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_20788_length_3593_cov_21.453363, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000122 DAOSZX010000000
VERSION     DAOSZX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20788_length_3593_cov_21.453363"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK196_07770"
     rRNA            complement(<1..346)
                     /locus_tag="PK196_07770"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(1338..1568)
                     /locus_tag="PK196_07775"
     CDS             complement(1338..1568)
                     /locus_tag="PK196_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41351.1"
                     /translation="MASLLKEGGRIYYVSDNVRYAGEVVPVDLILSEFAEAAGLQVKT
                     IYKLPNGKVNSSQQMGRHGRCEVQKCVYCWEK"
     gene            complement(1577..1765)
                     /locus_tag="PK196_07780"
     CDS             complement(1577..1765)
                     /locus_tag="PK196_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41352.1"
                     /translation="MDPGDVRTMLTFAAFSILEEISYTRKDGQFLRWDRRPGMVRATF
                     HKGKILAFEDALFTSTPL"
     gene            complement(1795..1932)
                     /locus_tag="PK196_07785"
     CDS             complement(1795..1932)
                     /locus_tag="PK196_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41353.1"
                     /translation="MRDELFLRHILDEIVLLREIGRDLTYEDLLRDPVRQHAIVRAIE
                     N"
     gene            complement(1925..2245)
                     /locus_tag="PK196_07790"
     CDS             complement(1925..2245)
                     /locus_tag="PK196_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HON41354.1"
                     /translation="MAAQVRRNKKSEFVANLEEVLPLLRERFGVAKIGIFGSTARGEE
                     RPDSDVDVLVEFAPGQTTFRNFMELAFYLEELFGRRVDLVTEQGLSRYLRPYVEQDVI
                     WCDA"
     gene            2290..2415
                     /locus_tag="PK196_07795"
     CDS             2290..2415
                     /locus_tag="PK196_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41355.1"
                     /translation="MNVAVHGKGLPVLLLCLGVEANETPVDAPPPSHSAATATRM"
     gene            complement(2755..2937)
                     /locus_tag="PK196_07800"
     CDS             complement(2755..2937)
                     /locus_tag="PK196_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41356.1"
                     /translation="MAHWTITLKREDAAIVVRGEGDDPHAPDRIDSEVRGDPRGVYRA
                     LLDRVAPGPWSFLLHR"
     gene            complement(2969..3202)
                     /locus_tag="PK196_07805"
     CDS             complement(2969..3202)
                     /locus_tag="PK196_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41357.1"
                     /translation="MRKASRKVVEATILRLCEGRYLTLDDLADLLNRSKDSLRNHYIN
                     PMLEDGRIEAKYKNVPTHPLQGYRTVAGTENEE"
BASE COUNT          750 a         1147 c          945 g          751 t
ORIGIN      
        1 aagttttcgc gcctgctgcg ccccgtaggg cctggattcg tgtctcagaa tccaactccg
       61 ggctcttgct cccacaaccc gtaccgatta tcggcttgtt gggccgttac cccaacaaca
      121 acctaatcgg ccgcagatcc atcctaaggc gccggagctt tcggttacag ggcattccag
      181 cgtctgtaac ctatagggta ttatcctcag tttcccgagg ttatcccctt ccttagggca
      241 ggttatccac gtgttactga gtagtacgcc gagggccgaa ccctctcgac tcgcatggct
      301 taatcgaacc ccgatagcag tgacctctgg caggatcaac cagaatttgg aagtatgcac
      361 actactaaac ctgggaggaa ttagcggtta ctgaacaaat ttccgcattg ccaatgccaa
      421 cgtcagaatc aacccgcgtc agagcgcggt ctcgttgatt ctccatcaac agggaaacaa
      481 tgtttgttat ctatctattt aaacatatgt ttcctgtatc aggtccgaaa cccattgctc
      541 gaaccttcat acgattcgtg gttacaggga agactgcacc tgaaccggtg cagaacttca
      601 acccgtgcaa ggacatatgt ttaccacttc taatatataa actttaccct tgcacgggac
      661 cccccggtcc gccgacctgc acccgattca cccggtgggt gcaacctgcg cgcacagacg
      721 gcacatggct gaacctgggg ggttataaac accgtcaacc ctttatcaga cccgaatcaa
      781 ccttcgggcc ctcacaacgg ttgagaggcc agggatcaga ccacccgggg ggctgcacga
      841 tcccttgccc tgacaacaca gccttacaat gttgtcatca atgttatata tacatttccc
      901 tgccccgtcg gatccaggcc gatggcctgg acccccacga aggtcggggc aatccccggc
      961 ttccgtgtcg agaacacaga ttccggggag aaacactccc gacctgacca tcacatgttg
     1021 atgccgcggc tatttataca tttaccccgc cggcggctca aaaacgggga tcaaacccgg
     1081 atgatcgtct cagaccttaa gaagtgcccc aacccgcccg gcgggaagag aggcggatct
     1141 ccatgcgcgc cggtgcacat gacacggatt atgcccgatc aacaccctcc gggccgggca
     1201 caggggcagg ggaccgccgg ggcgctccac ccatccacgc accaggcccc ggcgcgcagg
     1261 gcgccgggag gcgggaaacg atcctctccg gcaaccggca tacgcagacc ctggctggac
     1321 cgcgcagcgg cgcccggcta cttctcccag cagtagacgc acttttgcac ctcgcatcgc
     1381 ccgtgccttc ccatctgctg gctgctgttc acctttccat tgggcagctt gtagatggtc
     1441 ttgacctgta atcccgccgc ttcggcaaac tccgacagta tcaggtccac aggaaccacc
     1501 tccccggcat accgcacgtt gtcgctgacg taatagatgc ggccaccctc tttcaagagg
     1561 ctcgccatct cgaagattac aaaggagtgc tcgtaaaaag cgcatcttcg aaggcaagga
     1621 tcttcccttt atggaaggtc gctctgacca tccccggccg ccggtcccat cgcaggaact
     1681 gtccatcctt gcgcgtgtaa ctgatctctt cgagaatcga gaaggctgca aatgtcagca
     1741 tagtcctgac atccccagga tccacctgcc cgttcacgta tgccaggaac gcattcagtt
     1801 ttcgatcgcc cggacgatcg catgctgccg caccgggtca cgaagcagat cctcgtatgt
     1861 aagatctctg ccgatctcgc ggagaagcac gatctcatca aggatatgac ggaggaagag
     1921 ttcatcacgc atcacaccag atcacatcct gttcgacata gggccgcagg tatcggctga
     1981 gcccctgttc ggtgacgagg tccacccggc gcccgaaaag ttcctccaga tagaaggcaa
     2041 gttccatgaa gttccgaaac gtcgtctgcc ccggcgcaaa ctcgaccagc acatccacgt
     2101 cgctgtcagg ccgctcctct cctcgtgcgg tagacccaaa gatgccgatc ttcgcgacgc
     2161 cgaaccgttc ccggagcagc gggagcactt cttcgaggtt cgcgacgaac tcgctcttct
     2221 tgttccggcg cacctgagca gccatatggg gtctctcctc ttcttgatcg taactggggg
     2281 tgaggaggta tgaatgttgc cgttcacggg aaaggtctgc ccgttctgct cctctgtctc
     2341 ggggtcgaag cgaatgagac cccggtcgac gcacccccgc catctcatag cgccgccacc
     2401 gcaacaagga tgtgattttg aagggcattg gcaacaaacg agtcgtcttc ctctttgttc
     2461 ccacaaattt aagtaaagac ctgggcgagg acgcgtaact cgacgcacgt ctagaatccc
     2521 gggaacatac atcagtcttt tgatttattc ctgcatctcg ccggagcggg atatggattc
     2581 tgctgtcagc cggggggtcg aaggccccgg cctcattccc tcgcgggatc gcatcttccg
     2641 ggagttcctc cggctccggc agcgggggca gatcagtctc caccgagcag ccggggtgca
     2701 gcagcgcctc gatataggcg cagtcggcga tccagtagag gtcgcgctca cccatcatcg
     2761 gtggagcaga aacgaccacg ggccgggcgc gacccggtcc agcaacgccc ggtatacccc
     2821 gcgggggtcg ccgcggacct cggagtcgat cctgtcgggg gcgtggggat cgtcgccctc
     2881 accccggacg acgatcgcgg cgtcctcgcg cttcagggtt atcgtccagt gcgccataga
     2941 tgatcgtggt gccggcggca gaccaccgtc actcctcgtt ctccgtcccg gccaccgtcc
     3001 ggtatccctg gagaggatgg gtggggacgt tcttgtactt cgcctcgatc ctgccatcct
     3061 ccagcatggg attgatgtag tggttccgaa gcgagtcctt gctccggttc agcagatcag
     3121 ccagatcatc aagggtcagg tacctcccct cgcagaggcg gagaattgtt gcttcgacta
     3181 ctttcctcga cgctttccgc acagatccaa ccggtcttgc aatctcgcgc aatgtatcca
     3241 aatgtttgga gcttgcgatc aaatgttcga agctttctgg caaatgttcg gagctccccg
     3301 gcaaatgttc ggagctcggc ttcgaacaga tcccaggagc tccgaacatt tcaccctcca
     3361 tcgggtggcg gccgggacgg taatagaacg tgctccggcc cgacccctcg ctcatcaaaa
     3421 gccctttctc gaccaggccg tgaagagcca cggtgatgtc gtgcgggtgc tctttcgtca
     3481 cctctttgag cctgccgtga ttcacgcatc cttcacaacg agcagtcacc agagcaagcc
     3541 gctcgacctc cccaagttca ctgaaagcat ccccgatctc ggccttaagc tct
//
LOCUS       DAOSZX010000123         3572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_20923_length_3572_cov_21.522320, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000123 DAOSZX010000000
VERSION     DAOSZX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20923_length_3572_cov_21.522320"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..1034
                     /locus_tag="PK196_07810"
     CDS             411..1034
                     /locus_tag="PK196_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41358.1"
                     /translation="MSSKKEVTIGITINLDNYENIRLEVEGDAETREDVENLILFLDG
                     ILARLGRGDPATAERVDAYRRRVLSAGPTAPEGAARKEVSAKYPEPEEREELLIAEAP
                     AKPLAREEPIPDRAEPTTRLQSERQEPLLTRRKIPEKPEEKPQAQPASPPVRAEPPEQ
                     ETVPAKPSPAQEEDVCEVCGAPVPKSQAKLSQLFMSRTLCKKCMERP"
     gene            1115..2260
                     /locus_tag="PK196_07815"
     CDS             1115..2260
                     /locus_tag="PK196_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HON41359.1"
                     /translation="MKKNLLMWDETLFRDPEVFEIDYVPEQFNHREAQIQELAFQIRP
                     GLRGGRPLNTICRGIPGTGKTTSVKKVFSEIEDATRKLVPVYINCQIDNTRFAIFSQI
                     YRRVTGRAPPSSGTSFKRVFEALARFLQKEDLVLLVALDDANYLLYKNEINQVLYTLL
                     RSHEAYPHVRIGVIAIVSDMSVTLQNEVDVRVASVFRPTEIYFPPYSTAEVHDILKER
                     VIQGFYPNVLSQEMLDLVVEQTLKSGDLRVGIDLLKRAALNAERDARRSIEEGDICKA
                     YEVSRYLHLAFSLRALKAEERKVLAGIAEMSGREEADMRTGDVYRFVKERTGVSYTKF
                     YEMVQKFDTMRLLNLHYYRQGRGRTRLISLRYDPERVLEHLGKVQSI"
     gene            2310..3257
                     /gene="mch"
                     /locus_tag="PK196_07820"
     CDS             2310..3257
                     /gene="mch"
                     /locus_tag="PK196_07820"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HON41360.1"
                     /translation="MLSVNELALDIFEELFEYAEEFCAVPHELDNGARVVDCGVSTSG
                     GYLAGRRFTEICMGGLGEVDIAMGRIRDVPIPFIEISTDFPAVACLGAQKAGWTVNVN
                     KYFAMGSGPARALSLKPRHTYEVIDYEDEFDYAVICLESDHLPNAGVMEHIADACNVD
                     VANTCAVVAPTASLVGSIQVAGRCVETAVYKLNELGFDTKKIIAGIGHAPIAPVKKDG
                     TKAMGSTNDATIYHGSIMLTMKAPEIKDYLEKIPSNKSRGYGKPFYEIFKEANFDFYQ
                     IDTSLFSPAEVVINELSEGAVYHVGAVNPEVTLKSFGFL"
BASE COUNT          813 a          973 c         1041 g          745 t
ORIGIN      
        1 taccgggaag aaacggggag tttatatcac agaaccgttg atttgatggc ctcatacgta
       61 gaaaagggag ggaggatcgc tctggaaggg caggcggtgc atcagcccct tgcatcatca
      121 tcactcagtg ggggtaactc tcatcatctg caacctcccc gcccgggata ttaaaactcc
      181 ccggatagac cctccgcgtg ttatggcgaa cctccacgca tccttctttg cggcctgcag
      241 ggtgtgcctg ctgattgccc gcgggggatc gattaatgcc ccgccgaaga ttgccagcac
      301 aggttctgca gcccgcgatg tttttttttg cggtttcatc atgccaatcc gcggccgtct
      361 gagggccacc ggtggaactg tatattctgg aatccaatat ttatttagat atgtccagca
      421 agaaggaagt taccatcggg atcaccatca acctcgataa ctacgagaac atacggctcg
      481 aggtcgaggg tgatgcagag acgcgggagg atgtggagaa cctcatcctc tttctggacg
      541 ggatccttgc cagactcggc cgaggagatc ccgcgaccgc agagcgtgtg gatgcgtacc
      601 ggcggcgggt tctctcggca ggaccgacgg caccggaagg tgcagcccgg aaggaggtct
      661 ccgccaaata cccggaaccc gaagagcgtg aagagctcct gattgcagag gctccggcaa
      721 agccgctggc gagggaggaa ccgatccccg accgggcaga acccaccaca cgcctccaat
      781 cggaacgcca ggaacctctg ctgacccgga gaaagattcc ggaaaaaccg gaagaaaagc
      841 cgcaggctca gcctgcatct cccccggtga gagcagagcc gccggagcag gagacggtgc
      901 cggcaaagcc ctcccccgcg caggaagaag atgtctgtga ggtctgtgga gcgccggtcc
      961 cgaaatcgca ggcgaaactc tcacagctct tcatgagccg gacgctctgc aaaaaatgca
     1021 tggaaaggcc ctgaaccatc ccgggatacc cgtgcaaagg gaggtgcacc tcgacctgga
     1081 gaatataaat actacggtgg acgaggagtg tataatgaag aagaatctgc tcatgtggga
     1141 cgagacgcta tttcgcgatc ccgaggtctt cgagatcgat tacgtccccg agcagttcaa
     1201 ccaccgtgaa gcgcagatcc aggaacttgc cttccagatc aggccggggc tgcgtggcgg
     1261 caggccgctt aacaccatct gccggggcat cccgggaacc ggtaagacta ccagtgtcaa
     1321 gaaggtgttt tctgagatcg aggatgcaac caggaaactc gttccagtat acatcaattg
     1381 tcagatcgac aacacaaggt ttgccatctt ctcccagatc taccgccgcg tcaccggccg
     1441 ggcgccgcca tcctctggaa cctcttttaa acgggtcttc gaagccctgg ctcgtttcct
     1501 gcagaaggag gatcttgtac tgcttgtggc gctcgatgac gcgaactacc ttctctacaa
     1561 gaatgagata aaccaggttc tatacaccct cctgcgctcg cacgaggcct acccgcatgt
     1621 tcggataggt gttattgcca tagtcagcga tatgtcggtc acgctgcaga atgaggtcga
     1681 tgtcagggtt gcgtcagtat tccggccgac tgagatatac ttccccccct actcgacggc
     1741 ggaggtccat gacatcctca aggaacgggt tatacaggga ttttacccga acgtcctcag
     1801 ccaggagatg ctggatctcg ttgtcgagca gacgctgaag agcggcgacc tccgtgtcgg
     1861 gatcgatcta ctcaaacgtg ctgcactgaa cgccgagcgg gatgcgcgca ggtcgatcga
     1921 ggagggggat atctgcaagg catacgaggt ttccaggtat cttcaccttg cattctcgct
     1981 tcgggcgctc aaagccgagg agcgcaaggt tcttgccggg atcgcggaga tgtcgggccg
     2041 cgaggaggca gatatgagaa ccggagacgt ctaccgcttc gtaaaggaga ggactggcgt
     2101 cagttacacc aagttctatg agatggttca gaagttcgat accatgcggc tcctgaacct
     2161 tcactattat cgtcagggaa gggggcggac acggctgatc agcctcagat acgaccccga
     2221 acgcgtgctt gaacacctgg gaaaggtgca atcgatttaa taactcttat ctgtttcacc
     2281 accgtttgta ctctgatgag gttttccaca tgctcagcgt gaacgaactt gcactggata
     2341 tttttgagga acttttcgaa tacgccgaag agttctgtgc cgtcccgcac gaacttgaca
     2401 acggtgcacg ggtagtcgac tgcggtgtca gcacttccgg tggctacctg gcaggacgaa
     2461 ggtttaccga gatctgtatg ggcgggcttg gtgaggtcga cattgcgatg ggccggatca
     2521 gggatgtccc catccccttc atagagatca gcacagactt tccggcggtt gcgtgccttg
     2581 gggcccagaa agctggctgg acggtcaatg tcaacaagta cttcgccatg ggtagcggtc
     2641 ctgcgcgggc gctctctctc aagccgcgcc acacatacga ggttatagac tacgaggatg
     2701 agtttgacta tgcggtcatc tgcctggaga gcgatcatct ccccaacgcc ggcgttatgg
     2761 agcatatcgc cgatgcctgc aacgtggatg tggccaacac ctgtgcggtt gttgccccaa
     2821 cggcatcgct tgtcggttcg atccaggttg cgggccggtg cgtcgagacg gcggtctaca
     2881 agttgaacga actcgggttt gacacaaaga agatcatcgc gggtatcggg catgcgccta
     2941 tcgcgcccgt aaagaaggat ggcacaaagg cgatgggcag caccaacgac gcaacgatct
     3001 accatggcag cataatgctg acgatgaagg cgccggagat caaggattat ctcgagaaga
     3061 tcccgagcaa taaatccagg ggttacggaa aaccgttcta tgagatcttc aaggaggcaa
     3121 acttcgactt ctaccagatc gacacctcgc tcttctcgcc ggcagaggtg gtcataaacg
     3181 aactatccga gggtgctgtc taccatgttg gggctgtcaa ccccgaggtt accctgaagt
     3241 cgtttggctt cctgtgaact accccggcgg caagaccggg gacttcccgc tccgccccat
     3301 tcacccccgc aagttaaaac actttttttt atctccagcg tccttttttg ggctgttgaa
     3361 cctctacttc cagggttgtg ccgggttttg attaaccctt gccggggcgt tcccaggagg
     3421 tctgtgaatt accgctgaca gaaccaccat ctgcgggtag ccctggggtt tttgcgagag
     3481 caggatgtgg ggggctggtc ggcgaaaggc gttcaatgcg agtgatttga gagcaccggt
     3541 gaactggctt gaggatcgac gggcatacta aa
//
LOCUS       DAOSZX010000124         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_21301_length_3515_cov_23.042775, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000124 DAOSZX010000000
VERSION     DAOSZX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21301_length_3515_cov_23.042775"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1122)
                     /gene="wecB"
                     /locus_tag="PK196_07825"
     CDS             complement(43..1122)
                     /gene="wecB"
                     /locus_tag="PK196_07825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HON41361.1"
                     /translation="MKVASIVGARPQFIKCAPVSRELRKEHREVLIHTGQHYDHGMSG
                     VFFEELGIPKPDYNLGIGSGTHGHQTGAMLAAIEDVLDGERPDVVLVYGDTNSTLAGA
                     LAAAKLNIPVAHVEAGLRSFDRRMPEEVNRVLTDHCSEILFCPTETAVSNLAAEGIRN
                     GVFLVGDVMVDAMNYNRGIAEERSRILEDVGVEPGDYLAVTVHRPSNTDSRESMAAIL
                     GALGEAGRPVVFPVHPRTRKFLGEYGLLGKIPGNVILTEPLGYLDMIRLMAHAEKILT
                     DSGGIQKEAYLLGVPCITLRENTEWVETVESGWNVLVGAHRDRILDAVRHFSPAARQK
                     NLFGEGNASLLIRKFLGRWLDTRTG"
     gene            complement(1126..2559)
                     /locus_tag="PK196_07830"
     CDS             complement(1126..2559)
                     /locus_tag="PK196_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HON41362.1"
                     /translation="MSSRLESIINARGPIRKVGVVGMGYVGIPAAVLFADAPEFEFVY
                     GFQRDSPSSGYKIDMLNRGESPLRGEEPGLEELLVKVLDAGKFRCTSDFSRIAECDAV
                     TLAIQTPFKNPKDLIPDFSALSEGLRQVGRHLTKGALVVLESTVTPGTTAGMAREILE
                     EESGLAAGEDFALAHAPERVMVGRLIRNIREHDRIVGGIDDVSTARAAELYRPVLATG
                     RIITMTATAAEVTKTAENAFRDLQIAAVNQLALHCEAMGVNVYDVRRGIDSLKGEGIT
                     RAVLWPGAGVGGHCLTKDTWHLERGAVLLGGDLWYPHGTESIFGAARRINEFMPKHMV
                     HLTIEGLKRAGKSAEGAKVALLGWAFIQNSDDTRNTPAEPYLEAMEEAGADVRVHDPY
                     VDEYPGIEVSHDIDAALAGADVITIFTGHRQYFSLEPARAAELSGSEHPVIVDGRNVI
                     DPDAFIEAGFIYKGIGRGDKNSHPIRR"
     gene            complement(2573..3460)
                     /locus_tag="PK196_07835"
     CDS             complement(2573..3460)
                     /locus_tag="PK196_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HON41363.1"
                     /translation="MMGRNHARVYSELKAVDSLYLYDINEKAARDLADAFGATVSTGV
                     EDLLAKVDAVSICVPTPFHFSVAGQALDAGVPFLIEKPICATAEESRQLIGMIPEDLV
                     VGVGHIERFNPIVPEIKKIVRDPLYVEMKRHNPASSRVSGSSVVEDLMIHDIDIMRNV
                     LLPEGSYRLAGSGNQDVCSALFSFNETPVYLSASRKSSKKIRMVYIEEEEFTIEGDFM
                     AQEVYIHRKPGQYAAEDERYVQENIIEKVLVNKQEPLKLELSTFLECVARGREFPVTP
                     GQALLNMEICEEISRCFAV"
BASE COUNT          655 a         1135 c         1004 g          721 t
ORIGIN      
        1 ataaatataa cctgtttttg gcaaggcgct gaaaagagcc cctcacccgg tccgcgtatc
       61 cagccacctg ccgaggaact tcctgatcag aagactggca ttcccctccc caaagaggtt
      121 cttctgccgc gccgccggtg agaaatgccg aacagcatcg agaatccgat cccggtgcgc
      181 accgacaagc acgttccacc cgctctcgac cgtctcgacc cattcggtat tctcacggag
      241 ggtgatgcag ggcacgccga gcaggtaggc ctccttctgg atgccgccgg agtcggtcag
      301 gatcttctcc gcatgcgcca tcagcctgat catatcaagg taaccgagcg gttcggtcag
      361 gatgacgtta ccgggtatct tcccgagaag accgtattct cccaggaact tccgcgtccg
      421 gggatggacc gggaagacga caggtcgccc ggcctccccc agggcgccca gtatggcggc
      481 catactctca cggctgtcgg tgtttgacgg ccggtggacg gtgaccgcca ggtagtcccc
      541 cggttcgaca cccacgtcct ccaggatccg ggaacgttct tccgcgatac cgcggttata
      601 gttcatagca tcgaccatga cgtcccccac caggaagaca ccattcctga tcccctcggc
      661 cgcaaggtta gagaccgccg tctccgtcgg gcagaagagg atctccgagc agtggtcggt
      721 gagcacccgg ttcacctcct cgggcatccg gcggtcgaaa ctcctgagcc cggcctccac
      781 gtgcgccaca ggtatattga gttttgctgc cgcaagcgct cctgcaagtg tggagtttgt
      841 gtcaccgtag accaggacca cgtccggcct ctctccatcc aggacgtcct cgatcgccgc
      901 aagcatcgca cccgtctggt ggccgtgcgt ccccgatccg atccccaggt tgtagtcggg
      961 tttcgggatg ccgagttcct cgaagaagac ccccgacatc ccgtggtcgt agtgctgccc
     1021 ggtatggatg agaacctccc gatgttcttt ccggagttcc cgggataccg gtgcacactt
     1081 tatgaactgc ggcctggcgc cgacgatcga tgcgaccttc atggactacc gccggatcgg
     1141 atggctgttc ttgtcgcccc ggccgatgcc cttgtagatg aacccggcct cgatgaacgc
     1201 gtcggggtcg atgacgttcc tgccgtccac gatcaccggg tgctcgcttc cggagagttc
     1261 cgctgcccgt gcaggttcga gtgagaaata ctgcctatgg ccggtgaaga tcgtgatcac
     1321 atccgcaccc gccagggctg catcaatgtc gtgcgagacc tcgatccccg ggtactcatc
     1381 cacatacggg tcgtggacgc ggacgtctgc acccgcctct tccatcgcct caaggtatgg
     1441 ctctgccggg gtgtttcggg tatcatcgga gttctggatg aacgcccacc cgaggagcgc
     1501 caccttcgcg ccctccgccg acttaccggc gcgtttgagc ccctcgatcg tcaggtgcac
     1561 catgtgcttt ggcatgaact cgtttatcct gcgcgcggca ccaaagatcg attccgtccc
     1621 gtgcggatac cacaggtcgc cgccgaggag gacggcgcca cgctcaaggt gccaggtatc
     1681 ttttgtcaga cagtggccgc cgacacccgc acccggccag aggaccgcgc gtgtgatacc
     1741 ctcacccttg agcgagtcga tccccctccg aacatcgtag acgttgacac ccatcgcctc
     1801 acagtggagg gcaagctggt tgacggcggc tatctggagg tcgcggaagg cgttctcggc
     1861 ggtctttgtc acctcggccg cggtcgctgt catcgtgatt atcctgccgg ttgcaaggac
     1921 cggccggtag agttcagccg cccgcgctgt gctcacatca tctatcccgc cgacaatccg
     1981 gtcgtgttcg cggatgttcc tgatcagccg tccgaccatc acccgttcag gagcatgagc
     2041 aagcgcgaaa tcctcgccag cggccagacc agactcctcc tccaggatct cgcgggccat
     2101 cccggcggtt gtgccggggg tgacggtcga ctcgagcacc acaagcgctc cttttgtaag
     2161 atgccgccca acctgccgga gcccctcgga gagcgccgaa aagtccggga tcaggtcttt
     2221 cgggttcttg aacggggtct ggatggcgag cgtcacagca tcgcactccg cgatccggga
     2281 gaagtccgat gtgcaccgga actttccagc atcaaggacc ttcacgagca gttcttccag
     2341 acccggctcc tcgccgcgaa gcggggactc gccacggttg agcatgtcga tcttgtagcc
     2401 ggaagacggc gaatcgcgct ggaaaccata gacaaactca aactctggcg cgtcggcaaa
     2461 gagcaccgcg gccggtatgc cgacataccc catcccgacc acgcccacct ttctgatcgg
     2521 gccccgggca ttgatgattg actccaacct gctgctcata taaaaaccat ccttacaccg
     2581 caaagcatcg gctgatctct tcgcagatct ccatgttaag cagcgcctgc cccggggtga
     2641 ccgggaactc ccgtcccctg gcgacacact cgaggaatgt cgagagctcg agtttcaggg
     2701 gttcctgttt gttcacaagc accttctcta tgatgttctc ctggacatag cgctcgtcct
     2761 cggcggcgta ctgcccgggt ttgcggtgga tgtagacctc ctgtgccatg aagtcgccct
     2821 cgatggtaaa ctcttcctcc tcgatgtaga ccatcctgat cttctttgag gacttcctgc
     2881 tcgcggagag gtagaccggt gtttcattaa aggagaagag ggcgctgcat acgtcctggt
     2941 tgccgctccc ggccagccgg tatgagccct ccgggaggag gacgtttcgc atgatatcga
     3001 tatcgtggat catcaggtcc tcaaccacgg atgacccgct cacgcgggag gaggcagggt
     3061 tgtgccgttt catctcgacg tagagcgggt cgcggacaat cttcttgatc tcggggacga
     3121 tcgggttgaa acgctcgata tgcccgacac cgacgacaag gtcttccggt atcatcccga
     3181 tgagctgccg gctctcctct gccgtcgcgc agatgggttt ctcgatcaga aacggcaccc
     3241 ctgcatcgag cgcctgccct gccacagaga aatggaatgg tgtagggacg cagatgctca
     3301 cggcgtcaac ttttgcaagc aggtcttcaa cgccggttga gacggttgcc ccgaatgcgt
     3361 cagcaaggtc gcgtgcagcc ttctcgttga tgtcatagag gtagagcgag tccaccgcct
     3421 tcagttctga gtatacgcgg gcatggttcc tgcccatcat tccaacaccg attacacctg
     3481 cgtccaatct ttcaccgccg gcagcatgag tttgc
//
LOCUS       DAOSZX010000125         3506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_21366_length_3506_cov_18.276731, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000125 DAOSZX010000000
VERSION     DAOSZX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21366_length_3506_cov_18.276731"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>1467
                     /locus_tag="PK196_07840"
     CDS             58..>1467
                     /locus_tag="PK196_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON41364.1"
                     /translation="MSDDRIHALPAVRRLLGNPVSRSMLRFVTQDDDCGDRLTNAIRC
                     YQGSSDGLCWRCRLAGRMVGYTLHRSSQIFGVNEDDIKKGLDEQVFVRGLKNVLEGIA
                     RYGITMPQIVNAPFLVVWDFTHRCNLRCTHCYQDAQKALPRELDTEEAKRLVEELADA
                     GVVVIAFSGGEPLMRKDFFEVAAHARKHDLYVALATNGTLITPEMAGRIRDAGIEYVE
                     ISVDGKDAASHDAFRGMPGAFDRTIAGIRNCVEAGLYTCIATTVTRANYDQVPEIYRL
                     ASELGVKRLMCFNFIPTGRGVEMADQDIPPDDRRDLLHRILAWTRDGSGPEALSTAPQ
                     LASVALDEERGGVPVGHFYAGDAIQGRTALLADFIGGCGAGRIYCSIEPEGDLQPCVF
                     MPIRVGNVREKPFLEIWHSSEVLKDLRDRSHLSGACAACENRYVCGGCRARAWAYFGD
                     LHAPDPGCVNNRRCWEELRG"
     assembly_gap    1468..1567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1568..1908)
                     /locus_tag="PK196_07845"
     CDS             complement(<1568..1908)
                     /locus_tag="PK196_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HON41365.1"
                     /translation="MPNFCPECGNELISKNAEICPNCGVRLRGGSGKSPGIAALCSFI
                     FPGLGQVYNGDVGRGFLILLGTIVGSLFFLIPGLAVFIYGIYDAYATAKRMNAGEIPY
                     REANALHMVLFV"
     gene            2072..2452
                     /locus_tag="PK196_07850"
     CDS             2072..2452
                     /locus_tag="PK196_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HON41366.1"
                     /translation="MPGLDGGDAVSEVLSTILMVVVVILLAAIVGSLVFGIWPPMEPS
                     ITMVTATRSGENVTFMVHGGTDVERVTNITCWIGGPGAGNEEVPLEAKVGYFETRRLP
                     EPTRIVIVGTYPDGNSMVLFDEVV"
     gene            complement(2871..>3506)
                     /locus_tag="PK196_07855"
     CDS             complement(2871..>3506)
                     /locus_tag="PK196_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41367.1"
                     /translation="FQVSPISFFAILLAAAVIYALIRHRPTGIGSRDARLLAFFALAA
                     ALVMLSYARSLPWIQASPGIVPDMRYISPAYLPMLVIGVYALKHAGLDADGVREALRT
                     LFWLVVIDLPLIFVILQVIAGKSPAAQITFITTLTFIFLAGAAILYIAVLARRASPRS
                     IAYTVPVLMLLPLAWELVVDFRFATSCWEGYHFWIPVVEYIWYIQYTIFPL"
BASE COUNT          681 a          901 c         1143 g          681 t
ORIGIN      
        1 tgtcaaaagc gtctcccaga actataacgt cctgagggca ctgcaggagt gatacttatg
       61 tcagatgatc gtattcatgc acttcctgct gtccggcggc tgctcgggaa cccggtttcc
      121 cggagcatgc tccgcttcgt tacgcaggat gatgattgcg gagatcgtct taccaacgcc
      181 atccgctgct accagggttc gagtgatgga ttgtgctgga ggtgcaggct ggcgggccgg
      241 atggtggggt atacactcca ccggagcagc cagatcttcg gggtgaacga ggacgatata
      301 aagaaggggc ttgatgaaca ggtcttcgtg cggggactca agaacgtgct cgaggggatc
      361 gcgcgttacg gcatcacgat gccccagatc gtgaacgctc ccttcctggt ggtctgggac
      421 ttcacccacc gctgcaacct gaggtgcacc cactgttacc aggatgcgca gaaggcgcta
      481 ccccgtgagc tcgataccga ggaggcaaaa cgtctggtgg aggaacttgc ggatgccggt
      541 gtggttgtga ttgccttctc cggcggggag ccgctgatgc gaaaggactt cttcgaggtg
      601 gccgcccacg cccgcaaaca cgatctgtat gttgcgcttg ccaccaacgg gacgctcatc
      661 accccggaga tggccgggcg gatacgtgat gccggcatcg agtacgtgga gatctcggtt
      721 gacgggaagg acgcggcgag tcacgatgcc ttccgcggga tgccgggggc gtttgaccgc
      781 accatcgccg gtatacggaa ctgtgtggag gcggggctct acacctgtat tgcaaccacg
      841 gtgacccggg caaactacga tcaggtcccg gagatctacc gacttgcatc agaactcggt
      901 gtgaaacggt tgatgtgctt taacttcatc ccgacgggga ggggggtgga gatggccgac
      961 caggacatac ctcccgatga tcggagggac ctgctgcacc gcatcctcgc gtggacgcgt
     1021 gatggcagcg gccccgaggc tctctccaca gcgccccagc ttgcttctgt ggcgctagat
     1081 gaggagaggg gcggggttcc cgtcggccac ttctacgccg gggatgcgat ccagggccgg
     1141 acggccctcc tcgccgactt catcggcggc tgcggtgcgg ggaggatcta ctgcagcatc
     1201 gaacctgagg gggatctcca gccctgtgtg ttcatgccga tcagggtcgg gaacgtgcgg
     1261 gagaagccgt ttcttgagat ctggcactcc tcggaggtgc tgaaggatct gcgggacagg
     1321 agtcatctct ctggcgcctg cgccgcctgc gagaaccgct acgtctgcgg tggctgccgc
     1381 gcccgtgcat gggcatactt cggtgacctc catgcaccag acccggggtg cgtcaacaac
     1441 cgccgctgct gggaggaact cagggggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnacg aagagaacca tatgcagggc gtttgcctcg cggtacggga tctccccggc
     1621 gttcatccgt tttgctgttg cataggcatc gtagatgccg tagatgaaga cggccaggcc
     1681 ggggatgagg aagaagaggg aacctacaat cgtcccgagg aggatgagaa atccccggcc
     1741 gacatccccg ttgtagacct ggccgagacc cggaaagatg aaggagcaga gcgccgcgat
     1801 acccgggctc ttcccggagc ctccccggag tctgaccccg cagttcgggc agatctccgc
     1861 gttcttgctg atcagttcgt ttccgcattc ggggcagaag tttggcacca acacaccatc
     1921 ctgtgattac gaaggattat accgttaacg ccgacgatat acgttccggt ttcgtgcggc
     1981 agacgcggga gcggatcttt tgccggcggc atcccgccgc ccgactcgat acaggcagtc
     2041 ttttatggga ttggctggta tattcatcat aatgcctggc ctggatggcg gggatgcggt
     2101 ctcggaggta ttgagcacaa tcctgatggt ggtggtcgtg atcctgcttg cggcgatcgt
     2161 ggggtcgctg gtcttcggga tatggccgcc gatggagccg tcgataacga tggtgacggc
     2221 gacccggtcg ggggagaacg taacgttcat ggttcacggc gggacggacg ttgagcgggt
     2281 cacaaacatc acgtgctgga taggcggccc cggggcaggg aacgaggagg ttccgctgga
     2341 ggcgaaggtg gggtatttcg agacccggcg tctgccggaa ccgacccgga tcgtgatcgt
     2401 ggggacgtat cctgacggca actcgatggt cctctttgat gaggtggtct gatggaatct
     2461 ccgggttccg cgtccctgaa ccccggggcg ggtgctctgg ctttcagcag ttcgcttcaa
     2521 aagacagcat atttcagcac ttatacagca gttgctgtca tcaatcaggg cgtgaattaa
     2581 ggttttttac cgattccgcg gctgttttgt gggttttcat ccctgaatga cagcagtgcg
     2641 gagaacaccc agctgtatgc aaccgtccga aagtgtcaca gaaacagcca tcgggtgttt
     2701 tcaattttgc tgaaaacatc tacctgtacg taatatatct caatatatat gatagcattt
     2761 ccagtacagc ctccttattt cacgttttct cactgacagc aactgctgtc attccgcttg
     2821 aaaactgctg aaaatgctgt cagcgtcatg ccccccggca gccccgcccg ctatagcggg
     2881 aagatggtgt actggatgta ccatatgtac tccaccaccg gtatccagaa gtggtagccc
     2941 tcccagcagg atgtggcgaa ccggaagtcg acgacgagtt cccaggcgag ggggaggagc
     3001 atcagcaccg ggacggtgta ggcgatggag cgtggggagg cgcgtctggc gagaacggcg
     3061 atgtagagta tggcagcgcc ggcgaggaag ataaatgtga gggtcgtgat gaacgtgatc
     3121 tgggctgccg ggcttttgcc ggcgatgacc tggaggatga cgaatatgag ggggaggtcg
     3181 atgaccacga gccagaagag ggttctgagc gcttcccgga cgccgtcagc atcgaggccg
     3241 gcatgcttca gggcgtagac gcctatgacg agcatgggga ggtaggccgg ggagatgtaa
     3301 cgcatgtcgg ggacgatccc ggggcttgcc tggatccagg ggagggatct ggcgtaggag
     3361 agcatcacaa gggcggcggc gagggcgaag aacgcaagga gccttgcgtc gcgtgagccg
     3421 atcccggtcg gtctgtggcg gatgagggcg tagatgaccg cagcggcaag gagtatggcg
     3481 aagaacgaga tgggggagac ctggaa
//
LOCUS       DAOSZX010000126         3494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_21442_length_3494_cov_20.461471, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000126 DAOSZX010000000
VERSION     DAOSZX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21442_length_3494_cov_20.461471"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PK196_07860"
     CDS             <1..1161
                     /locus_tag="PK196_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HON41368.1"
                     /translation="VYNNVTEQRQREEQIRELMERAKKESERLAESAEALGNALSAMS
                     KGDLTTFVEAQNGNDPLHKVKMDYNTSLTAIRAVLADVAKAVEQVDMTTKEVSKSTDE
                     IIRATEQVAVSTQQSSEDARRQLEKIEEIGKDVNDLSASIEEIASTSQTVMEQATKSA
                     REGSEAASLGDVAAKKMQLVEGISKQTVDEINTLNNQMREINNIVKLIADIANQTNLL
                     ALNAAIEAARAGEHGRGFAVVAGEIRNLAGESKRASQDIEDLIRTIQASTERTTGSMQ
                     ASHQEIQAGIESVNKVIGSLNRIVDSVEVVAHGISEITKATEDQATSTNSVMQKMDEF
                     THMTKESLDRTEDMAALAEEVSASTEEVGSASHELASMAEQLRKAMTRFKLN"
     gene            1172..1654
                     /locus_tag="PK196_07865"
     CDS             1172..1654
                     /locus_tag="PK196_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HON41369.1"
                     /translation="MAASVDVVEFQLGKEHYALDIKIAREIVEMMPITPLPRAPPYLA
                     GIINLRGEITNIIDLRDLLGLPDSGESENRKIVVLMPEVAGGSNVGIIVDDVHSVISV
                     REDQIERINDSITSSISSYIKAIIKMGGAESAKTESLIIWLDVQKVIKDLGGNYHGAQ
                     "
     gene            1690..2484
                     /locus_tag="PK196_07870"
     CDS             1690..2484
                     /locus_tag="PK196_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HON41370.1"
                     /translation="MDEFASLQKTIERLLGIKCSNYKEDYIKRRLLSRMRLTNTADFE
                     AYHRYLLANPQEHELLRNALTINVTKFFRDPEVFEVLRREILPDLARRRDSIRIWCAG
                     CATGEEPYSIAILVHELLALRKDISVTIYATDIDTVVLQKAIAGVYDSKTLENVDEKR
                     IRKHFISRDDGTFEVRPHIKSLVRFREHDLMSGEAVARYLDIVTCRNVTIYFTEKQKN
                     DLTYLFYSALASGGYYVMGKTEYLGREVEHLFVPYNSAQKIFRKAA"
     gene            complement(2487..2744)
                     /locus_tag="PK196_07875"
     CDS             complement(2487..2744)
                     /locus_tag="PK196_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41371.1"
                     /translation="MDVTEVRTWVIFYLLSFVVLLVATLITSQGSQLWISLMLIIIVV
                     GVNFFLLVTELKRYQAKEAFLKRISKIVKDSKTEGDSISPK"
     gene            2911..>3494
                     /locus_tag="PK196_07880"
     CDS             2911..>3494
                     /locus_tag="PK196_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41372.1"
                     /translation="MDKGDLLSIIAALIIVSAVALLLRPPAAEPGSGVPETPVTTPTP
                     PITTPTLIPTPTIALPEPFRITYTTSVWDYPVCYLPENLTRYGGSDPPWKGAHRAVAF
                     AWIEESRGGITEAFYVPYSVWRLNCSVSATVWPEAARFRMALVELESGTIVEGAELYY
                     PGSIIKNVEMGGKEFYLIVSVDNIDSYRITLETLP"
BASE COUNT          860 a          939 c         1016 g          679 t
ORIGIN      
        1 gtttacaaca acgtcaccga gcagcggcag agggaggagc agatcagaga actgatggag
       61 agggcaaaga aagagagcga gcggctagcc gagagtgccg aagccctcgg aaacgccctt
      121 tccgccatgt caaaaggcga cctgacgacg tttgtggaag cgcaaaacgg gaacgatccg
      181 ctccataagg tcaagatgga ttacaacacc tcgctgacgg ccatccgtgc ggtccttgcc
      241 gacgtggcaa aggcggtcga acaggtggac atgacgacta aagaggtcag caagagcacc
      301 gatgagatca tccgcgccac ggagcaggtc gctgtctcga cgcagcagtc ttcagaggat
      361 gcacgccgcc agctcgagaa gatcgaggag ataggaaagg atgtcaacga cctctcggca
      421 tccatcgagg agattgcaag cacatcgcag acggtcatgg agcaggccac aaaatctgcc
      481 agagagggga gcgaagccgc ctctctgggt gatgttgcgg caaagaagat gcagctggtc
      541 gaggggatct ccaagcagac tgtcgacgag atcaacaccc tcaacaacca gatgcgcgag
      601 atcaacaaca tcgtcaagtt gatcgctgac atcgccaacc agaccaacct gctggcgctg
      661 aacgccgcaa tcgaggccgc acgtgcaggg gagcacggcc gcgggtttgc tgtggttgcc
      721 ggggagatca ggaaccttgc gggggagtcc aagcgggcaa gccaggatat cgaagacctt
      781 atccggacga tccaggcgag caccgagagg acgacggggt cgatgcaggc atcccaccag
      841 gagatccagg caggcatcga gagcgtcaac aaggttatcg ggagcctgaa ccggatcgtc
      901 gacagcgtgg aggttgtcgc ccacggcatc agcgagatca caaaggccac ggaggaccag
      961 gccacatcca caaacagtgt catgcagaag atggatgagt tcactcatat gaccaaagag
     1021 agcctggacc gcaccgagga tatggcagcg ctcgccgaag aagtcagcgc ctcgacggag
     1081 gaggtcggga gcgcctccca cgaactggca agcatggccg aacagctcag aaaagcgatg
     1141 acgcggttca agttgaactg aggaggagac gatggcagca tccgtggatg tggtggagtt
     1201 ccagctcggg aaggagcatt acgcacttga tattaagatt gcgcgggaga tcgtggagat
     1261 gatgccaatc accccccttc cacgtgcacc cccatacctt gcgggcatca tcaacctcag
     1321 gggagagatc acaaacataa tcgacctgcg cgaccttctt ggcctccctg attcagggga
     1381 atcggagaac cggaagattg tggtgctgat gccggaggtg gcgggcgggt cgaacgtcgg
     1441 gatcatagtt gatgacgttc acagcgtcat atccgtccgc gaggatcaga tcgagcgaat
     1501 caatgacagc atcacctcat ccatcagctc ctacatcaag gcgatcatca agatgggggg
     1561 tgcagaaagc gcaaagacgg agagtctcat aatctggctt gatgtccaga aagtgatcaa
     1621 agatcttggt ggaaactatc atggcgctca gtaggccggg ggttgccggc acgagtgtga
     1681 aactgtccaa tggatgagtt tgcctcgctc cagaagacca tcgagcggct gctcggcata
     1741 aagtgctcga actacaaaga ggattatata aagcggcgcc tcctctccag gatgaggctt
     1801 acaaacaccg ctgattttga ggcttaccat cgctacctgc tggcaaaccc gcaggagcat
     1861 gaactcctcc gcaacgctct caccataaac gtcaccaaat tcttccgcga ccctgaggtc
     1921 ttcgaggtgc tgcgccggga gattctgccg gatcttgcac gccgcaggga ctcgatcagg
     1981 atctggtgcg cgggctgcgc cacgggtgag gagccctact cgatcgccat cctggttcac
     2041 gaactgctcg cgctccgtaa agatatcagc gtaacaatat atgcaaccga tatcgatacc
     2101 gttgtgctgc agaaagccat tgcgggggtt tatgattcaa aaacgcttga gaacgtggat
     2161 gaaaagagga ttcgcaaaca tttcatcagt cgcgacgatg gtacgtttga agtccgccct
     2221 catatcaaga gcctcgtgcg gtttcgtgag cacgatctga tgtctgggga ggccgtcgcg
     2281 aggtacctcg atatcgtgac ctgccgaaac gtcaccatat actttaccga gaagcagaag
     2341 aacgatctta cgtatctgtt ttactcggcg cttgcttctg gcggctacta cgtcatgggt
     2401 aagaccgagt acctgggaag agaggttgaa cacctttttg tgccgtataa cagtgcacag
     2461 aagatcttca ggaaggcagc ctgaagttat tttggtgaga ttgagtcccc ttccgtctta
     2521 ctatccttga cgatctttga gattcgctta aggaacgctt ccttcgcctg gtagcgcttt
     2581 agttcggtga caagcaggaa gaagtttaca ccgacgacga tgatgatgag catgaggctg
     2641 atccagagct ggctgccctg gcttgtgatg agtgttgcca caaggagcac cacgaacgat
     2701 agcaggtaaa agatcaccca ggtgcgcact tctgtgacgt ccatgcttcc tccatcaaga
     2761 cgctggtgcg gccaggtatt aacgcttatg ttttacgtgt tctatcttct gagatgaggc
     2821 tgatcctgcc ccgaatgcag gacgcttgat ctgctgcttt cgtggcggta aacccgaagg
     2881 tctttttaat gtccacacca ccttaaatct gtggataaag gagatctcct cagtatcatt
     2941 gctgccctga tcatcgtctc cgcggtagca ctgctccttc gaccgccggc agcggagccg
     3001 ggatcagggg ttccggagac ccctgttacg acgcccaccc ctcccataac cacccctacc
     3061 cttatcccga caccgaccat tgccctgcct gaaccgttca ggatcaccta cacgaccagc
     3121 gtctgggact accccgtctg ttacctcccg gagaacctta ccaggtatgg tggctccgac
     3181 ccgccctgga agggagccca ccgggcagta gcgttcgcct ggatagagga gagcaggggc
     3241 gggatcacgg aggccttcta cgttccatat tcggtgtgga ggctaaactg cagcgtctca
     3301 gcaacggtgt ggcccgaggc ggcccgcttc cggatggctc tcgttgaact tgagagcggg
     3361 acgatagttg aaggggctga actctactat ccgggcagca tcatcaaaaa cgtcgagatg
     3421 ggagggaagg agttttacct catcgtgagc gtggataata tcgattcata ccgcatcacc
     3481 ctggagacgc tccc
//
LOCUS       DAOSZX010000127         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_23074_length_3279_cov_13.001861, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000127 DAOSZX010000000
VERSION     DAOSZX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23074_length_3279_cov_13.001861"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK196_07885"
     CDS             complement(<1..185)
                     /locus_tag="PK196_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HON41373.1"
                     /translation="MTIDVLAFSASPRRHGNSETLLDWVLEGMEAEGCSIEKIVIPEI
                     DIRPCRGCNACERLNRCV"
     gene            890..1069
                     /locus_tag="PK196_07890"
     CDS             890..1069
                     /locus_tag="PK196_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41374.1"
                     /translation="MLVFGESARSMPVEERIQKVSVVFQDPDDQLFMPTLYDDVAFGP
                     INMGLSQDEVDRRVG"
     gene            1088..1549
                     /locus_tag="PK196_07895"
                     /pseudo
     CDS             1088..1549
                     /locus_tag="PK196_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1615..1851)
                     /locus_tag="PK196_07900"
     CDS             complement(1615..1851)
                     /locus_tag="PK196_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41375.1"
                     /translation="MKSAVYALSPLILFSWAILPGVPFAGLLLLGWFCLSTYFGVWIF
                     HEKSKDRAVFVALATGVVLAIYLHRWIVDPWLAL"
     gene            1871..2038
                     /locus_tag="PK196_07905"
     CDS             1871..2038
                     /locus_tag="PK196_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41376.1"
                     /translation="MDVDEEQEEVLDEADREEGDGPADQTLHQGDKGACCDKPDPVQS
                     VDLRRLSRKEG"
     gene            complement(2341..>3279)
                     /locus_tag="PK196_07910"
     CDS             complement(2341..>3279)
                     /locus_tag="PK196_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HON41377.1"
                     /translation="KEFADQITGWRFLVILALFLTIALVGTYSGVGSYERDLDRYAQQ
                     LATMDDRFDEPGRMMMPAKPPVEGVYSSMFLTLVSYGGLLALALGFDLVSKEKESRSL
                     KSLLSHPVYRDEIINGKALGGVALLALVVGSVLAISTALLLVFSIVPSPGDLWMILTY
                     AGVTHLFLVTFFSIALALSTLCRESGSALLLSVVVFILLVFLVPFATANIGTALMMEE
                     PDLAAYGGDTQSEGYQAEITAYYGQWDLIESAANLLSPQMAINALIQGIANAPSSGAT
                     LEDTLGEIWSSIAALTIYPVAFFAVAYTRFMRMDIR"
BASE COUNT          697 a          963 c         1021 g          598 t
ORIGIN      
        1 acgcagcggt tgagtctctc gcaggcgttg cagccgcggc acggcctgat atcgatctcg
       61 gggatgacga tcttctcgat ggaacagcct tccgcctcca ttccctcaag cacccagtca
      121 agcagggtct cggagttgcc gtggcggcgg ggcgaggccg aaaacgcgag gacgtctatg
      181 gtcatatatg atgtgttgtc ccggcggagg caaaaaacgt gccgggactg gtttcactgc
      241 accttctcct gcgacagccc gatcgcgagc gtgtagctga ggccgatcac cacgaacatg
      301 aacgggaaag ctgcggtgat cgccatcacc tggagcggat caagcccccg gtgaggggga
      361 ggacgcccat gatgccgtcg aggagccacc cggagacggt tctcccagta cccacccaga
      421 tcacggatgc aacgactgca acgagcgggg gacgagcggc gagaaccggc tccggggaag
      481 agaggcgatg atcttacctg acacactgca ccgtccggga cgtgaaccgg ggacgcggtt
      541 cggctgtcgg atcctgtaac tcttcctgag aagttacctc cttactatat acgatttctg
      601 tcacgatcct ctacacgtcg gggtgagtta acccattctg ctcatggggc gggctgcaaa
      661 cggccgggac atacggtccc gcggatcccc gtcatgcagg gtgatacccc tccctctgaa
      721 catgcaggta ttatcgccga aagaaacggt ttcgccggtt cattgagggg taatgtacag
      781 gttaaccgtg tttttttgct cgaaggagcc tttatgacaa ataacaaatt cttttaagtt
      841 tacgcaaatc ctccacctga acggctccat caagagtccc ggggacaacg tgctcgtctt
      901 cggcgagagc gcccggtcca tgccggtcga ggagcgcata cagaaggtca gcgtcgtctt
      961 ccaggacccc gacgaccagc tcttcatgcc caccctctac gacgacgttg cgttcggccc
     1021 gatcaatatg ggactttcgc aggacgaggt cgaccggcgt gtcgggtagg cccttgcgac
     1081 cgtcggccta tctgtgtttg atgaccgcgt tccccaccat atgagttccg gcgagaagaa
     1141 acgcgccgcg ctggcggccg ttctctccat ggagcccagg gtcctcgtcc tcgacgaacc
     1201 gaccgcaaac ctcgatccaa agacccgggc ccttctcatc gatctcatca acaggctgaa
     1261 ccgggagaag gggatcacca ccattgtcgc ggacggggac gtccacagca tcttctcgga
     1321 cagcaaactg ctgaaggaga acaacctcga agcgccggac gcctacaaac tcttccgcat
     1381 catgggttgt tacggctata ccaactgcaa cgctcttcct ctctcggtcg cggacgctcc
     1441 cggcgctctg gatgcgtcgc tcgaggagaa cgggggcgcg atccacctga ggcgggacgg
     1501 ccggactgcg gaagaactcc gcgaggtgtt cgagcggttc gagtactgag cggctcaaac
     1561 agcttttttc tccgggtaaa cgaccgtatc cccgttccgg ccggttcggg gccgttagag
     1621 agccagccag gggtcgacga tccaccggtg aagatagatc gcgagcacca cgcctgtcgc
     1681 gagagcaacg aagacggccc ggtcctttga cttctcgtgg aatatccata cgccgaagta
     1741 ggtcgagagg cagaaccagc cgaggaggag caggccggca aacggaaccc ccgggaggat
     1801 cgcccaggag aagaggatga gcggcgagag ggcatagacc gccgacttca tcgtctgctc
     1861 gaactcccgg gtggacgtcg acgaagagca ggaagaagtg ctcgacgagg ctgaccgcga
     1921 ggagggcgac ggccccgccg atcaaaccct gcaccagggt gataagggtg cctgctgcga
     1981 taaaccagat ccggtgcagt ccgtcgatct ccggcgcctc tcccgtaagg aggggtagca
     2041 tcgtgaatag aaccgatgcg actgcgacga tcgcgaagta gaatatgagg tcgtcgcgca
     2101 gcggtttttc attgagtttc tggaagaccc gcggggggcc ggtgatgatt gttcctgctg
     2161 acatgctcct cctcccggaa tgactgcagt catccgtccg gttccgatat cgatgaagga
     2221 gaagtcgatg ttctcctata aacgttctct gtccgaaccg tctacacgtc ggggtttcga
     2281 tctattttct ctcggtatta cgaaacgacc ggggcgcacg gttccctctc tcacgagcgg
     2341 tcaccggata tccatccgca tgaacctcgt gtacgcgacg gcgaagaagg cgacgggata
     2401 gatggtcagg gccgcaatac tagaccatat ctcgccgagt gtatcctcaa gggtcgcccc
     2461 cgatgacggg gcgttggcga tcccctggat gagggcgttg atggccatct gcggtgagag
     2521 gaggtttgca gcgctctcga tcaaatccca ctgcccgtaa tacgccgtaa tctccgcctg
     2581 gtagccttcc gattgggtat ccccgccgta tgcagcgagg tcgggttcct ccatcatgag
     2641 ggccgtgccg atattcgcgg tggcgaaggg gaccaggaag acgaggagga tgaagacaac
     2701 caccgaaagg aggagggcgc tgccgctctc ccggcagagg gtggagaggg cgagcgcgat
     2761 ggagaagaac gtcacgagga agaggtgggt gacgccggca taggtcagga tcatccagag
     2821 atcgccgggg gagggcacga tcgagaatac gaggagcagc gcggtggata tagcgagcac
     2881 gctcccgacg acgagggcaa gcagcgcaac gcccccgagc gccttgccgt tgatgatctc
     2941 gtcgcggtag accgggtggg agagaaggct tttgagcgac cgggactcct tctccttcga
     3001 gaccaggtcg aacccgagcg caagcgcaag gaggcccccg taggagacca gcgtgagaaa
     3061 catcgaggag taaacgccct cgaccggcgg ttttgcgggc atcatcatcc gccccggttc
     3121 atcgaaccgg tcatccatcg tcgccagctg ctgggcatac ctatccaggt ctctctcgta
     3181 gctcccgacc ccgctgtagg ttcccaccag ggcgatggtg aggaacaggg cgaggatcac
     3241 cagaaaccgc cagcccgtga tctggtcggc aaactcttt
//
LOCUS       DAOSZX010000128         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_24691_length_3105_cov_19.184590, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000128 DAOSZX010000000
VERSION     DAOSZX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24691_length_3105_cov_19.184590"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..151
                     /locus_tag="PK196_07915"
     tRNA            67..151
                     /locus_tag="PK196_07915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Leu,seq:gag)
     gene            289..1020
                     /locus_tag="PK196_07920"
     CDS             289..1020
                     /locus_tag="PK196_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HON41378.1"
                     /translation="MLTDRERERYSRQIALLGEEAQERLSKARLAIVGAGGLGCPVAL
                     YLAAAGVGEIRLIDGDLVDLTNLNRQVLHTESDIGRPKVESAAAKLRAQNPGIRVAAI
                     QAALDEGNAAGYLAGVDLVIDAADNFAVRYTLNRAAIRMGVPLIHGAVSGFDGQAMTI
                     IPGRTACLECIFPAAPPGEEMIPVIGTTPGIIGLIQANEAIKYITGSGELLENRLLIW
                     DGRAGTMTTLAVERQEDCSVCGGGR"
     gene            1031..1447
                     /locus_tag="PK196_07925"
     CDS             1031..1447
                     /locus_tag="PK196_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HON41379.1"
                     /translation="MISVTEEDFDINAVIEQAKRPDLGALVTFLGTVRADEGVEVMEV
                     EAYREVAVRELETIRDEAFQRFPVAAVWIIHRVGRLRVGENILLIVVGAGHRHEAFAA
                     CEYVLERIKQSVPIWKKEIGDGGERWVPGETSGGGA"
     gene            1444..2658
                     /locus_tag="PK196_07930"
     CDS             1444..2658
                     /locus_tag="PK196_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HON41380.1"
                     /translation="MIRLQPCRKAYSKETQRAVPPEETLRLARERLPAAGITRVADIT
                     NLDRIGIPVFSSIRPTAEAGAISVYNGKGATPIEAEVSAMMEGIERYSAEIGDREVIM
                     GRCSEVSAEYNALDPADLILPDHVPADLVIPWVRGYDIVQQEEILIPAHAVFHPLPVK
                     MGGLFRTNTSGLASGNTLEEAVFHALMEVVERDAWSLAEVTRDTGPRIEGIEDGLAAD
                     LLARFEAAGVEVTLKDITSDTGIPTVAAVADDLVLRDPTLLTIGMGSHTSTGIAVLRA
                     LTEVAQSRLTQIHGAREDTDTADVRKRIGYERTKRLNRHWFADSESVSFADLPSFDSD
                     DFLTDIRHVIDRLRAVGLSRVIVVDLTRPEIGIPVVRVVVPGLEIYAMDQDRMGRRCR
                     DARRRRISGSKL"
     gene            2621..>3105
                     /locus_tag="PK196_07935"
     CDS             2621..>3105
                     /locus_tag="PK196_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HON41381.1"
                     /translation="MRGVVVFLGPSCDPAAARQILDADYRPPAKRGDVIRAAEEGARV
                     IGLIDGVFFQDSAVAHREILAALRAGVRVVGASSMGALRAAELDSLGMEGVGEIYRAY
                     REGRLVADDEVALLFDPETFAAISEPLVNIRATVQAAIECGVIGADTARALVEAAKEI
                     Y"
BASE COUNT          577 a          895 c         1095 g          538 t
ORIGIN      
        1 cggcagagcc tcgtgagggg cctaatgttt tattatattg cagtgcgttg tatatgggta
       61 gataaagcga gggttgccaa gccaggtcaa aggcgccagg ttgagggcct ggtctcgtag
      121 gagttcgtga gttcgaatct cacccctcgc atcggcactt tttctcttct ccgccagcgg
      181 caaatcgatg ttgggtgcgg ctttttgcaa acgcaatctt caaatcagcc gggagaccac
      241 ataaattgcg tattcaaagg gttgcaggat caaggaggat atccagcgat gcttaccgac
      301 cgggaacggg agcgctacag ccgccagata gcgctcctcg gggaagaggc gcaggagcgg
      361 ctttcaaaag ccaggctcgc catagtcggc gccggcgggc tcggctgccc ggtcgcgcta
      421 tacctggccg ccgccggtgt gggggagatc cggctgatcg atggtgatct cgtggacctg
      481 accaacctga acaggcaggt gctccacacc gagagcgata tcggccggcc gaaggtcgag
      541 tcggcggcgg cgaaactccg ggcgcagaac ccgggcatca gggtcgccgc gatccaggcc
      601 gccctggacg aggggaacgc cgccggctat cttgccgggg tcgatctcgt catcgatgcc
      661 gccgacaact tcgctgtgcg ctacaccctg aaccgggcgg cgatcaggat gggtgtcccg
      721 ctcatccacg gcgcggtcag tgggtttgac ggccaggcaa tgactatcat cccgggaaga
      781 acggcctgcc tggagtgcat attcccggcg gctccacccg gggaggagat gatcccggtc
      841 atcgggacga cgccggggat catcggtctt atccaggcaa acgaggccat aaagtacatc
      901 accggcagcg gcgaactcct ggagaacagg ctcctgatct gggacggccg ggcggggacg
      961 atgacgaccc tggccgtgga gcggcaggag gactgcagcg tctgcggggg cggcaggtga
     1021 ggagaagagc atgatcagtg ttacagaaga ggatttcgat ataaacgccg tgatcgagca
     1081 ggcgaagaga ccggacctcg gtgctctcgt caccttcctt ggcaccgtca gggccgatga
     1141 aggtgtggag gtgatggagg tcgaggccta ccgggaggtc gcggtgagag aacttgagac
     1201 catcagggat gaagcgttcc agagattccc ggtagcggcc gtctggatca tccaccgggt
     1261 tgggcgactc cgggtcggtg agaacatcct cctcatcgtc gtcggcgccg gccaccggca
     1321 tgaggctttt gcggcctgcg agtatgtcct cgagaggatc aagcagagcg tgccgatctg
     1381 gaagaaggag atcggcgatg gcggtgaacg ctgggtgccc ggcgaaacct ctggaggcgg
     1441 ggcatgatcc ggctccagcc ctgcaggaag gcctactcaa aggagaccca gcgtgcggtt
     1501 ccgcccgaag agacgctgcg cctggcgcgg gaaaggctgc ctgcggccgg gataaccagg
     1561 gttgcggaca taacaaacct ggatcggatc ggtatcccgg tcttctcgag catccggccg
     1621 accgcggagg ccggggcgat atcggtctac aacgggaagg gtgccacccc gatcgaggcc
     1681 gaggtctcgg cgatgatgga ggggatcgag cgctactctg cagagatagg cgaccgcgag
     1741 gtgatcatgg gccgctgcag tgaggtctcg gcggagtata acgccctcga tcccgccgac
     1801 ctgatcctac ccgatcacgt cccggccgat ctggtgatcc cctgggtccg gggttatgat
     1861 atcgtgcagc aggaggagat ccttatcccg gcccacgcgg tcttccaccc gctgccggtg
     1921 aagatgggcg gtctcttccg gacgaacacc agcgggcttg cctcgggcaa caccctcgag
     1981 gaggcggtct tccacgccct gatggaggtt gttgagcgcg acgcctggtc gctcgcggag
     2041 gtgacacggg ataccgggcc gcggatcgag gggatcgagg acgggcttgc cgccgacctg
     2101 cttgcaaggt tcgaggctgc gggggtggag gtcacgctca aggatatcac cagcgatacg
     2161 gggatcccca cggttgcagc ggtcgccgat gacctcgtcc tcagggaccc gacgctgctc
     2221 acgatcggga tggggtcgca taccagcacc gggatcgcgg ttctccgggc gctcacggag
     2281 gtcgcacaga gccggttgac ccagatccac ggcgccaggg aggataccga tacagcggat
     2341 gtgaggaaac ggatcggcta cgaacggacg aaacggctga accgccactg gtttgcggat
     2401 tcggagagtg tttcgtttgc tgacctgccg tcgtttgaca gcgacgactt cctcaccgac
     2461 atccggcacg tcatcgaccg gctcagggcg gtggggctgt cgcgggtgat cgtggtcgac
     2521 ctcaccagac ccgagatcgg gatcccggtc gtccgggttg tcgtgcccgg cctggagatc
     2581 tacgcgatgg accaggaccg gatgggcagg aggtgccgtg atgcgaggcg tcgtcgtatt
     2641 tctgggtcca agctgtgacc cggcggcagc ccgccagatc ctggacgccg attaccggcc
     2701 acccgcaaaa cggggcgacg tcatccgggc agcggaggag ggggcacggg tcatcgggct
     2761 gatcgatggc gtcttcttcc aggattctgc cgtcgcccac cgcgagatcc ttgccgcgct
     2821 gagggcgggg gtgcgggtcg tcggcgcctc aagcatgggg gcgctccgtg cggcggaact
     2881 cgacagcctg ggtatggagg gtgtcggtga gatctaccgc gcctaccgcg aggggagact
     2941 tgttgctgat gacgaggtgg cgctcctctt cgatcccgag acattcgcgg cgatctcaga
     3001 gccgcttgta aacatccggg cgaccgttca ggcggcgatc gagtgcggtg tgatcggcgc
     3061 cgataccgcc cgggcgctgg tagaggccgc aaaggagatc tactt
//
LOCUS       DAOSZX010000129         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_25597_length_3017_cov_18.777178, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000129 DAOSZX010000000
VERSION     DAOSZX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25597_length_3017_cov_18.777178"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1832)
                     /locus_tag="PK196_07940"
     CDS             complement(288..1832)
                     /locus_tag="PK196_07940"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HON41382.1"
                     /translation="MKRTAFFTDSHAKKNVTVFDTTLRDGEQTPGISFTLEEKLAIAE
                     QLSKIGVHAIEAGFPASSETERQSVKAVVGLGLSSQVCGLARSLTSDVDACIDCGVDM
                     VHVFIPTSDVQREYTIRKTREQVLDTVNEIITYARDHVDQCMFSPMDATRTDWDYLIQ
                     VCRTAMDAGATIINVPDTVGVITPGAMKRLIERIGSEVNCPLDVHCHNDFGLAVANTL
                     AAVEAGASQVQVTVNGLGERAGNADLAQTVMALTSIYGIDTGIRTTGLVETSRLVSRY
                     SGISIPPTQPIVGENAFAHESGIHSHGVITRSDTFEPGIMTPEMVGHRRRLKLGKHAG
                     KHAIRQMLTEVRIEPTDAQLDEIVQRVKAISGKGKRMTDADLYEIAESVMHLAPDEKT
                     LELQDVAIMTGNHVIPTASVRALVDGKEHIFSNVGNGPVDAAVKAILGIMPAPIQLKE
                     FNIEAISGGTDAIGHVTIAVEDEKGRVFDASAANDDIVLASVEAVVNAINLVCRMRKH
                     DRNQKA"
     gene            complement(2471..2839)
                     /locus_tag="PK196_07945"
     CDS             complement(2471..2839)
                     /locus_tag="PK196_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HON41383.1"
                     /translation="MTYTKNGKTFNCPIEAALDVIGGKWKPLILWRLCGGIKRFSELQ
                     RELPGVNAKMLTRQLRELEGDGLVMRTVYPEVPPRVEYAITGFGRTLVPVMEALCAWG
                     LEYLGIDDEGTCRCTKEGRD"
BASE COUNT          548 a          945 c          896 g          628 t
ORIGIN      
        1 ccccctctgg agggggcggg aggaggccgc ggtagcggcc gggcggtggg ggtgtttcca
       61 gcgacacgcg aagacagggg aaggggcgtt ccgccccctc cccgtcagcc cctcccccac
      121 gggggcgata tcccatagac gcactgccac ggggagtgca tcccctcatc aggtttcagg
      181 cgcgacacct cgaagattcg gttcagtctc cggacacggc catcgtgacc ctccaacggt
      241 atctggaaaa aagggtttgt ggaatgaggg aaggtgagaa agttctctca cgctttctgg
      301 ttccggtcgt gtttgcgcat cctgcagacc aggttgatcg cattgaccac cgcctctacg
      361 gaggccagga cgatgtcgtc gtttgcggcg ctggcgtcaa agacccggcc cttctcgtcc
      421 tcgacggcga tcgtgacgtg cccgattgca tcggtgcccc cggagatcgc ctcgatgttg
      481 aactccttca actggatggg ggccggcatg atcccgagaa tcgccttcac ggccgcgtcc
      541 acaggaccgt tgccgacgtt cgagaagatg tgctctttcc cgtcaacgag cgccctgacg
      601 cttgcagtcg ggatcacatg gttcccggtc atgatcgcga cgtcctggag ttccagggtc
      661 ttctcgtcgg gtgcaaggtg catgacgctc tccgcgatct cgtagaggtc agcgtccgtc
      721 atccgtttgc ccttcccgga aatcgccttc acccgctgga cgatctcgtc gagctgcgca
      781 tcggttggct cgatccgcac ctcagtcagc atctgcctga tggcatgttt cccggcgtgt
      841 ttgccgagtt tgagcctgcg ccggtggccg accatctccg gtgtcatgat cccgggctca
      901 aacgtgtctg agcgcgtgat aacgccgtgg gagtggatcc cgctctcgtg ggcgaaggcg
      961 ttctcgccga cgatcggctg cgttggcggg atgctgatcc cggaatagcg cgatacaagc
     1021 cttgaggtct cgaccaggcc cgtcgtccgg atgccggtat cgatcccgta gatggacgtg
     1081 agggccatca ccgtctgtgc aaggtcggcg ttccccgccc gttctccgag accgttcacc
     1141 gtcacctgga cctgcgatgc ccccgcctcg accgccgcca gggtgtttgc cacagcaagc
     1201 ccgaaatcgt tgtggcagtg gacgtcgagc gggcagttca cctcgcttcc gatcctctcg
     1261 atgaggcgtt tcatcgcgcc cggcgtgatc accccgacgg tgtccgggac gttgatgatc
     1321 gtggctcctg catccatcgc ggtgcggcag acctggatca ggtagtccca gtcggtcctg
     1381 gtggcatcca tgggggagaa catgcactgg tcgacgtggt cacgtgcata cgttatgatc
     1441 tcgttgaccg tatcgagcac ctgctcgcgt gtcttgcgga tggtgtactc cctctggacg
     1501 tcggatgtcg gtatgaagac atgaaccata tcgacaccgc agtcgatgca cgcatccaca
     1561 tcggatgtga gcgaccgcgc cagtccgcag acctgtgacg agaggcccag acccacgacg
     1621 gctttgacgc tctggcgttc ggtctccgag gacgcgggga accctgcctc gatggcgtgg
     1681 actcctattt ttgagagctg ttcggcaata gcgagcttct cttcgagcgt gaacgagatg
     1741 cccggtgttt gttcaccgtc gcgcagcgtg gtgtcgaaaa cagttacatt ctttttcgcg
     1801 tggctatcgg tgaagaaggc agtacgcttc agcgtttcca ggtgcttcag cgtttgtagg
     1861 tttcataact actcgttatc agcgaaggta tataaagaat ttgggcaacc cgccggcggt
     1921 ctccggggtg gccatcaaag attagaaaga ttgggccggt gaatctccat cgccacagac
     1981 cgcgctctac ccccgggata tttttgcaaa gacaacattc cggggagaga tgggggggcg
     2041 tttccaccat cgatacactg caacggccag gatgagcaga tccgcgggct cgatgtgaac
     2101 atcctgctca tccccctgtg cacggccatc cgggtcaatc gccacgcact gcccgccccc
     2161 tctggagggg gcgggaggag gccgcggagg cggccgggcg gtgggggtgg acccagaggt
     2221 taaaacaccg ccacaggaga cgccgagaca ggggcggggg cttttccacc cccctccccc
     2281 acggggcgat tctccatcga tacactgcca gtgaagagca tcccctcacc ccgttcccgg
     2341 cgagaaaatt ccctgaaaga cctgtctccg tcccccggac acgactatcc ggactatcgc
     2401 caaacacttg ccctccccct cgaggtgccg gggcgcagcg gaacctcaag atcgctggtt
     2461 tgatggctcc tcagtctctc ccttcttttg tgcaccggca cgtgccctca tcgtcgatac
     2521 cgagatactc aaggccccag gcacagagtg cttccatcac cggtaccagc gtccttccaa
     2581 acccggtgat ggcatactcc acccgtggcg gcacctccgg gtagaccgtc cgcataacaa
     2641 gaccgtcccc ctcgagttcc cggagctgcc ttgtgagcat ctttgcgttg acccccggca
     2701 gttcgcgctg cagctcggag aaccgtttga taccgccgca gaggcgccat agtatcagcg
     2761 gcttccactt ccccccgatt acatcgagcg ccgcctcgat ggggcagttg aaggtcttgc
     2821 cgttctttgt gtaggtcatg gttacaccga tggaagtatc ttaccaaaat atgtgtatat
     2881 cctatatatc cccaaaggat ctatttctac cataacacag ggtccgtcag cggccccatc
     2941 gcggctggcg gaccgccgtg cgaggtgata accatggaga agagagagat caacggaaac
     3001 tttgccctcc cgatgcc
//
LOCUS       DAOSZX010000130         2993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_25827_length_2993_cov_22.261402, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000130 DAOSZX010000000
VERSION     DAOSZX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25827_length_2993_cov_22.261402"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..2656)
                     /locus_tag="PK196_07950"
     CDS             complement(5..2656)
                     /locus_tag="PK196_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HON41384.1"
                     /translation="MPEDANIFSVEHEPAVKKVLYWCDRCNVPLIGRTCGCRASSREI
                     ALLPPHDVRPALAGDITLIKRLLADRFGDIPLPHVVLLNKTGGIDRADLVIVNGERFG
                     WLTFDPIARQYSLDITPEAISWILPHATSGVVDLDTEPAVRAHRGRIGGKRFPLSTPV
                     TDGTVIITYKNRFGTGVVRDGQVRVKELLPVEPGIRPDPGWETVIERNRYHLKNLERN
                     AVRTIKKHMNDRPCVNVSFSGGKDSTAVLHLARKAGVEKAFFIDTGLELPETVEFAES
                     QGVEIIRKGGDFFEAVKKAGPPAKDRRWCCKLLKLRPLKIYLAGTGPCVTIQGNRWYE
                     SWNRADLDETSQNPANPLQVNVSPIRNWRALEVFLYLWWQGVPINPLYEKGLERIGCY
                     LCPAVLESEYEMLRGLHPELTGPWDEFLARWAEKNGFPEAYCRWGLWRWRALPPKMRE
                     VCRDHGIPLNDDFTLKAAAPEDGAEMTETKSPTTREIEFNPDEIRRDFPILDDDIIYL
                     DNAATTFSPEPVVEALVEFEHHYRANVGRGVHRLTQIATQRYWHAHEKVARFIGGGDG
                     VTVFTKNTTEAINMVAQGLSWRPGDRVATTILEHHSNLLPWRALAKQGVEVDLIGIDA
                     NYALDLNALEEALSGGSVRLVAVTHASNVLGVTTPVPEIARLCREHGALLLVDAAQSL
                     PHMPVDVSSLDCDFLCFSGHKIFGPTGTGVLWMREALIEPPILGGGMVASVTSDGYVP
                     AEGYLRYEAGTPNISGGIALGAAVDYLSAIGMDRIHRHEERLTARLIEGLSATEGVRV
                     YAGKRPDARIGVVSFIIDGVHPQEAAQMLDEEADIMVRSGHHCCQPLMDYLGLPEGTV
                     RASLAAFTTEQEIDLLIAAVDEISRGR"
     gene            2767..>2993
                     /locus_tag="PK196_07955"
     CDS             2767..>2993
                     /locus_tag="PK196_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HON41385.1"
                     /translation="MPSCCVNSLGGKVLLMDATPKEVNDYIKRHCREYYEISPGFVFK
                     DIRVLLKEPMLVGLQVKRGKILLPFTKRCPNL"
BASE COUNT          582 a          941 c          853 g          617 t
ORIGIN      
        1 ggctctaccg tccccggctg atctcatcga cggccgcgat caggagatcg atctcctgct
       61 ccgtcgtgaa tgccgccagg cttgccctga ccgtcccctc cgggagaccc aggtaatcca
      121 tcagcggctg gcagcagtgg tgccccgaac gcaccatgat gtccgcctcc tcatcaagca
      181 tctgcgcggc ctcctgcggg tgaacaccat cgatgatgaa cgagaccacc cctatccggg
      241 catctggcct ctttccggca tataccctga cgccctcagt tgcagagaga ccctcgatca
      301 gccgcgccgt caggcgctcc tcgtggcggt ggattcgatc catcccgatc gccgagaggt
      361 agtccacggc ggcgccgagc gcaatacccc cgctgatgtt tggcgttccc gcctcgtagc
      421 ggaggtaacc ctccgccggg acgtagccat ccgatgtcac gctcgcgacc atcccgccgc
      481 cgagaatcgg cggctcgatg agcgcctccc tcatccagag gacaccggtc cccgtcggcc
      541 caaagatctt gtgccccgag aaacatagaa agtcacaatc gaggcttgag acgtcgaccg
      601 gcatatgtgg gagcgactgc gccgcatcca ccaggagcag cgccccatgc tcccggcaga
      661 gccgcgcgat ctccggtaca ggtgtagtca ccccgaggac gtttgaggcg tgtgtcacgg
      721 caacgagcct gacactgccg ccggagaggg cctcctcgag cgcgttaagg tcgagcgcgt
      781 agttggcatc gatcccgatc agatcgacct caaccccctg ctttgcaagc gccctccatg
      841 gaaggaggtt tgagtggtgc tcaaggatgg tggtcgccac acggtcgcca ggcctccagg
      901 agagcccctg cgcgaccatg ttgattgctt ccgttgtgtt cttcgtaaat accgttaccc
      961 catccccgcc gccgataaac cgggccacct tctcgtgggc atgccagtag cgctgtgtcg
     1021 ctatctgtgt cagccggtgg acgccccggc cgacgtttgc cctgtaatgg tgctcaaact
     1081 cgacgagcgc ctccaccacc ggttccggtg agaacgtagt cgccgcgtta tcgaggtaga
     1141 tgatgtcgtc atccaggatc gggaagtctc tgcggatctc atccgggtta aactcaattt
     1201 cacgagttgt tggactcttc gtttctgtca tctctgctcc atcctccggc gctgccgcct
     1261 tcagggtaaa atcgtcgttg agggggatgc cgtgatccct gcagacctcg cgcatcttcg
     1321 ggggcagagc ccgccaccgc cacagccccc accggcaata ggcctccgga aacccgttct
     1381 tctccgccca gcgggccagg aactcatccc atggccctgt aagttccgga tgcaaccccc
     1441 gcagcatctc gtactcgctc tccagcaccg ccggacagag gtaacatcca atccgctcaa
     1501 gccccttctc gtaaagcggg tttatgggga caccctgcca ccagaggtat aggaagacct
     1561 caagcgcccg ccagttccgg atcggcgaga cgttcacctg cagcgggttg gccgggttct
     1621 ggctggtctc gtcgagatca gcgcggttcc aggactcata ccagcggttc ccctgtatgg
     1681 tgacgcacgg cccagtcccg gccaggtaga tcttcaaagg ccggagtttc aggagtttgc
     1741 agcaccagcg gcggtccttc gccggcggcc cggctttctt gaccgcctcg aagaagtcac
     1801 cgcctttgcg gatgatctcg acaccctgag actccgcaaa ctccaccgtc tcggggagtt
     1861 caagccctgt atctatgaag aacgccttct ccaccccggc cttccgggcg aggtggagga
     1921 cggcggtgct gtccttgcct ccggagaacg agacgttcac gcacggccgg tcgttcatgt
     1981 gttttttgat ggtccgaacg gcgttgcgct ccaggttctt caagtggtag cggtttctct
     2041 ctatcaccgt ctcccagccc ggatcaggcc gaatgccggg ctcaacgggg aggagttctt
     2101 ttacccggac ctggccgtcc ctgacgaccc ccgtgccgaa ccgattttta taggtgatta
     2161 tgaccgtccc atctgttacg ggggtcgaaa gaggaaaccg tttcccgccg atacggcccc
     2221 tgtgagcgcg caccgcgggc tcggtatcga ggtcgacgac gccgctggtt gcgtgcggga
     2281 ggatccagga gatcgcttct ggcgtgatat ccaggctgta ttgtcgcgcg attgggtcaa
     2341 acgtgagcca gccgaatcgt tcgccgttca cgatcacaag atcggccctg tcgattccac
     2401 cggtcttgtt gagcagcaca acgtgcggga ggggtatatc cccaaatcga tcggcaagaa
     2461 gacgtttgat gagagttata tctcccgcaa gcgcgggccg gacgtcatgc ggcggcagca
     2521 gcgctatctc tcttgaactt gccctgcagc cgcatgtgcg cccgatgaga ggaacattgc
     2581 accggtcgca ccaatagagg acctttttta ctgccggttc gtgctccacg ctaaatatat
     2641 tagcatcttc aggcattaag tcgaccggca ggtcatttgc gaaactgtct gaaaacaact
     2701 atcgcgcgcg gtgagggtaa ctgaagatgg caaggtattt attcgtacaa agacgaacaa
     2761 ggtaagatgc catcctgttg cgtaaacagc ctcggtggaa aggtgctcct catggacgcg
     2821 acaccgaagg aggtaaacga ctacatcaag cgacactgca gagagtacta cgagatatct
     2881 ccaggttttg tctttaagga tattcgggta ctccttaagg agccgatgct tgtagggctg
     2941 caggttaaaa gaggcaagat actgctgcct tttacaaagc gttgtccaaa tct
//
LOCUS       DAOSZX010000131         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_25872_length_2987_cov_24.638131, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000131 DAOSZX010000000
VERSION     DAOSZX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25872_length_2987_cov_24.638131"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK196_07960"
     CDS             <1..950
                     /locus_tag="PK196_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON41386.1"
                     /translation="RRLMKTIEVDGLSKSFGHIKAVDNISFDVEEGECFGLVGPNGAG
                     KTTTLKLLATVLNPDSGSARIYGYSILDQKDEVRANLGMVFEEPSLDVQLTGRDNLDF
                     HACMYHMPREERRKRIAEVLMVVGLEERADIPVKDYSGGMQRRLEIARSMLTHPRVLL
                     LDEPTVGVDVQTRRFMWDHVRELNHNHGVTVLLATHYIEEAEYLCDRIAIMNMGRIMA
                     IGTPEELKDSVGKSVVSLSLSEGSSRDFIRMLSGLSWVENVRENNGQIEIGLKRENIN
                     LAEIVDLAQGHDLIITAIKMRKPRLEDVFFQYAGSLVEE"
     gene            950..1939
                     /locus_tag="PK196_07965"
     CDS             950..1939
                     /locus_tag="PK196_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON41387.1"
                     /translation="MNVLEVEGLTKTFGEFVAVDQIGFTVSEGEIFGFLGPNGAGKST
                     TIKILTTILNPTSGEARVCGYDVVRQMDEVRRSIGIVFQDPSIDRFLTGRENLRYHAV
                     MYHMPSKMIEERIDEVLDLLDLRGREDIPIKDCSAGIQRRFEVARGFMTHPRLLFLDE
                     PTVGLDMQARKRLWSYINKLREEEEITIFLTTHYIEEADHLCDRVGIIDHGKIVAIDT
                     PQNLKDVVGGETILVEAANRRNGRFVDALRELEWVTAIEDGGQELRLSVIGGESRIPQ
                     ILTVASQTGTLITSVELVRPSLEDVFLYYTGKTMREEEGSLQDLRKARMRRRR"
     gene            1944..2765
                     /locus_tag="PK196_07970"
     CDS             1944..2765
                     /locus_tag="PK196_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41388.1"
                     /translation="MEKITVSGGSRTSRLIDPLPIYIMWLREMKRIFRVKARLVSNLL
                     MPVLFLAFLGLPLSMVSANGSEMFGLPPGVDFLDFLAPGIIGMTILFSSLMGGASVVW
                     DKEFGFLKEVMVAPVSRFSILLGRQAGAMTISIGQALIILLIATLMGVNLQNSPIVLL
                     SIVFMVLTSATFMGLGLILATKLGEMEGFMAILSLFQMPLFFMSVALFPLDLMPKWLG
                     YIICLNPFTYGVDGLRGALVGISTFPLALDLFVIFACAAVLAGLGTHFFSTMEAD"
BASE COUNT          688 a          705 c          907 g          687 t
ORIGIN      
        1 cacgacgttt gatgaaaaca attgaagttg atggattgag caagagtttt ggccatatca
       61 aagccgtgga taacatatcc tttgatgtgg aggaggggga gtgttttggt ctggtcggcc
      121 ccaacggtgc ggggaagacg acgacactca aacttctggc cacggtgctc aatcccgact
      181 caggttcagc caggatatac ggatacagta ttctcgacca gaaggatgag gtcagggcaa
      241 accttggcat ggtcttcgag gagccgagtc tggatgtcca gcttacgggg agggacaatc
      301 tggattttca tgcatgcatg tatcacatgc cccgggagga gaggagaaag aggatcgccg
      361 aggtcttaat ggtggtgggt cttgaggaga gggcggacat accggtcaag gattactcgg
      421 gagggatgca gcgtcgactc gagatagcaa ggagcatgct cacgcatccc cgcgtcctcc
      481 tgctggacga accgacggtg ggcgtggacg tccagacgag gaggttcatg tgggaccatg
      541 tcagggagtt gaatcacaac catggggtga ccgtcctgct tgccacccat tacatcgagg
      601 aggcggagta cctctgcgac aggatagcga tcatgaacat gggaaggatc atggccattg
      661 gcacaccaga ggaactcaaa gactctgtcg gaaagagcgt tgtttcgcta tccctcagcg
      721 agggctcttc ccgagatttc atcaggatgc tttcaggact ctcctgggtt gagaacgtcc
      781 gtgaaaataa cggtcagatt gagatcgggc ttaagcgcga gaatatcaat ctggctgaga
      841 tcgttgatct tgcccagggg catgacctca tcattacagc cataaagatg cgtaaacccc
      901 gtcttgagga cgtcttcttt cagtatgcgg gatctctggt ggaggagtga tgaacgttct
      961 tgaagtggag ggtctgacaa aaacgtttgg tgagtttgtt gccgtcgatc agatcggttt
     1021 cacagtttcg gaaggtgaga tattcggttt cctcggtccc aacggcgccg ggaaatccac
     1081 caccataaag attctcacca ctatcctcaa cccgacctcc ggagaggcca gggtgtgcgg
     1141 atatgatgtg gttcgccaga tggatgaggt gagaagaagc atcgggattg tgtttcagga
     1201 cccctcgatc gacaggttcc tcacaggcag agagaacctt agataccacg ctgtcatgta
     1261 ccatatgccc tcaaaaatga ttgaagagag gatcgacgag gttctggacc tgctagacct
     1321 ccggggcagg gaggacattc caatcaaaga ttgttctgcc gggatccaga ggcgattcga
     1381 ggtggcccgg ggtttcatga cccatcctcg gttgttattc ctggacgagc ccacggtggg
     1441 tctggatatg caggccagga agaggctctg gagttatata aacaaactca gggaagaaga
     1501 ggagataacc atcttcctaa ccacacacta catcgaggag gctgaccacc tctgcgaccg
     1561 tgtgggcata atcgatcacg ggaagatcgt ggccatcgat accccgcaga acctgaagga
     1621 tgtcgtcggg ggtgagacga tcctggtaga ggcggcaaac cgccggaacg ggaggtttgt
     1681 tgatgcactc cgggagttgg aatgggtaac ggccatcgaa gacggcgggc aagaactcag
     1741 gttgagtgtg attggcgggg agagccggat accgcagatc ctgactgttg caagccaaac
     1801 cggcacactc ataacgtcgg ttgagctggt caggccaagc ctggaggatg tgttcctgta
     1861 ctacacgggt aagacgatgc gtgaggaaga agggagcttg caggatctaa gaaaagcaag
     1921 gatgcgaaga aggagatgaa ggtatggaga aaataacggt ttctgggggg tccaggacaa
     1981 gccgcctgat agatcccctg ccgatataca tcatgtggct gcgggagatg aagaggatct
     2041 tcagggtgaa ggcaagactt gtttcaaatc tacttatgcc agttctcttc cttgcattcc
     2101 tgggcctgcc tctgagcatg gtatcggcga acgggagcga gatgtttggg cttccgccgg
     2161 gagtggattt cctcgatttt ctggcgccgg ggattattgg gatgaccatc ctcttcagtt
     2221 ctctcatggg cggcgcctcg gttgtatggg ataaggagtt tggtttcctg aaagaggtga
     2281 tggtcgctcc tgtcagtcgg ttctcaattc tgctcggccg ccaggcgggg gcgatgacca
     2341 tatccatagg acaggctctc atcatcctcc tgatcgcgac tctgatgggg gtcaatctcc
     2401 agaactcgcc cattgtctta ctctcgatcg tgttcatggt tctgaccagc gccacattta
     2461 tggggctggg tctgatactg gccacaaaac tgggtgagat ggagggtttc atggccatac
     2521 tgtcgctgtt tcagatgcca ctcttcttca tgtctgtcgc cctcttccca ctggatctca
     2581 tgccgaagtg gcttggatac atcatatgcc tcaacccctt cacgtatggc gtggacggac
     2641 tgagaggagc gctggttggg atctcgacat tcccgcttgc ccttgatctc tttgtcatct
     2701 ttgcatgtgc ggcagtgctt gcagggcttg gtacgcactt cttctccacc atggaggctg
     2761 actgagagat tcttcaaaga gatgttctat cggggtctgg atctgctgcg agagatccgc
     2821 ggtgaccctt gcctgacaga gtcggtcaag gatcctgcag taggggtaga gagttcggaa
     2881 ttgcagaaaa acgcggccca gaacctctgc tgcatcccgg agttagagtc cattggccgc
     2941 gatctgcttc cagcccacaa aacggaggta ggtgttttca cggagtc
//
LOCUS       DAOSZX010000132         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_27565_length_2835_cov_20.094245, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000132 DAOSZX010000000
VERSION     DAOSZX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27565_length_2835_cov_20.094245"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2708)
                     /locus_tag="PK196_07975"
     CDS             complement(<1..2708)
                     /locus_tag="PK196_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HON41389.1"
                     /translation="MYKNLSPDECRTTAWHALQVDEVERLLESPTDGLTEKEVARRRE
                     VFGENALPKKRPPTVFEVFLRQFKSPLIYVLLAAGIISIIFVDLTDAIFIFAVLLLNA
                     VVGTSQEVKAEKGATALQEMLKIHARVIRGGRERVVPAEDLVPGDRVVLESGDRVPAD
                     IRIIRARSLTVDESLLTGESHAVSKTPDPVDASLPTSDRRSMLYAGTTVMTGRCTGVV
                     VATGQNTEIGQIAKSVTESEGGKPPLIIRMEDFSRKIAIAILAATGVLAIIVLGQGMP
                     VLDVFFLAVALAVSAIPEGLPVALTVALSIAASRMAARNVIVRVLAAVESLGSCTLIA
                     SDKTGTLTVNQQTARVIALPTGERFSITGAGYAGEGEVLEEDGEPVAGPNRSRLEQLA
                     RAAAICNEAALERTDGGNWTHHGDAIDVAFLALAYKLGLDPAAVREGAPSVAEIPFES
                     ERRYAAVWYRDGEEIRVAVKGAVETVLPFCRSMAAGGDRSLPLDPGGVQEELELLTSQ
                     GYRVLAVAHGREERDISGENPQVPPLELLGLVGFIDPIRPDVPDAVRRCREAGVDVVM
                     VTGDHPATALAIARELEIADSPDQVITGAEMGDTGDSITPEFIERVKRARVFARVTPL
                     QKLRIVEAYRESGAFVAVTGDGVNDAPALRSANIGIAMGSGTDVAKDTASLIITDDNF
                     ASIVAGIEEGRYAYDNVRKVVYLLISTGFAEVLLFMLALALGLPLPLFAVQLLWLNLV
                     TNGIQDVALAFEGGEPDVMKRPPRKPDEGIFNRLMIEETLLSGTLIAVVALCAWHWML
                     GNGWDEFAARNMLVLLMVLFENIHVFNCRSEYISAFRVPISRNYFLIAGVIAAQGIHI
                     LALHIPFFQNVLQVQPVSLGEWVFLLALASTVVIVMEIFKAVRKHRP"
BASE COUNT          544 a          891 c          869 g          531 t
ORIGIN      
        1 ggcctgtgtt ttctgacggc cttgaagatc tccatcacga tcaccaccgt tgaggcaagg
       61 gcaagcagga agacccactc tcccagggat accggctgca cctggagcac gttctggaag
      121 aagggaatgt ggagtgcgag gatgtgtatg ccctgcgcag cgatgacgcc ggcaatcaag
      181 aagtagttgc ggctgatagg aacgcggaac gcggagatgt actccgaccg gcagttgaag
      241 acgtggatgt tctcgaatag caccatcagc aggacaagca tgttcctggc cgcgaactcg
      301 tcccagccgt tgccaagcat ccagtgccag gcgcagagcg ccaccaccgc gatgagggtg
      361 cctgagagga gcgtctcctc tatcatcagg cggttgaaga tcccctcgtc gggctttcgc
      421 ggcggccgtt tcatgacgtc gggctccccg ccctcgaatg cgagcgccac atcctggatc
      481 ccgtttgtca ccaggttcag ccagaggagc tggactgcaa aaagcggcag cggcagaccc
      541 agggccaggg cgagcatgaa gagaagaacc tccgcaaacc cggtcgagat caggaggtag
      601 acaactttgc ggacgttgtc gtaggcgtag cgcccctcct cgatacctgc aacgatcgag
      661 gcgaagttat cgtcggtgat gatcagcgat gccgtgtcct tggcaacatc agtccccgag
      721 cccatcgcga tcccgatatt agcgctccgg agcgccggag cgtcgttgac gccgtccccc
      781 gtgaccgcaa caaacgcccc gctctcccgg tatgcctcga cgatccggag tttctgtaac
      841 ggtgtcaccc ttgcaaagac ccgggcgcgc ttcacccgct cgataaactc aggtgtgatc
      901 gagtcgccgg tatcgcccat ttccgcgccg gtgatcacct gatccggtga atcggcgatc
      961 tccagttccc gtgcaatggc aagggctgtt gcggggtggt cgccagtcac catcaccaca
     1021 tctacccctg cttcacggca ccgtcggacg gcatccggga cgtcaggccg gatcgggtcg
     1081 atgaacccga ccaggccgag gagttcaagg ggaggaacct gcgggttctc tccagagata
     1141 tctctctcct ccctaccatg ggccacagca agcacccggt agccctgtga ggtcaggagc
     1201 tcgagttcct cctgcacgcc gccgggatcg aggggaagac tcctgtctcc cccggcagcc
     1261 atactccggc agaacgggag gacggtctcg acggccccct tgaccgctac ccggatttcc
     1321 tccccatcac ggtaccagac agcagcgtag cgccgttcgg actcaaacgg gatctcggca
     1381 acggatggcg caccctcccg gacggcagcg gggtcaagcc cgagtttgta ggcgagtgcg
     1441 agaaacgcca cgtcgattgc atcgccatgg tgtgtccagt tgcctccgtc ggtccgctcc
     1501 agcgccgcct cgttacagat cgccgccgcc cgggcaagtt gttccagtct ggagcggttg
     1561 gggccggcca ctggctcgcc gtcttcctcg aggacctcac cctcacctgc gtaaccggcc
     1621 ccggttatgc tgaacctctc accggtgggg agggcgatga ccctggcggt ctgctggttc
     1681 acggtgagcg tccctgtctt gtcgcttgcg atcagagtgc agcttccaag actctccacc
     1741 gctgcgagga cccggacgat gacgttgcgt gccgccatcc gcgacgccgc gatcgagagg
     1801 gcaacggtca gtgcgaccgg cagcccctcg gggatcgccg agaccgccag ggcgacggca
     1861 aggaagaaga catcaaggac aggcatcccc tggccgagca cgatgatagc aagcacccct
     1921 gtggcagcaa ggatggcgat ggcaatcttg cgtgagaagt cttccatgcg gatgatgagc
     1981 ggtggtttgc cgccctcaga ctccgtgacg ctctttgcga tctggccaat ctctgtattc
     2041 tggccagtcg cgaccacaac tcccgtgcac cgccccgtca tgacagtggt gccggcatac
     2101 agcatgctcc gccgatcgct ggtgggcagc gacgcgtcca cggggtcggg tgtctttgat
     2161 accgcgtggg actcgcctgt caggagcgac tcgtcgacgg tgagtgaccg tgctctgatg
     2221 attcggatat cggcggggac gcggtcgccg gactccagga cgacacggtc gcctggaaca
     2281 aggtcttcgg caggcacaac cctctcccgg ccgccccgta tcacgcgtgc atggatcttg
     2341 agcatctcct gcagcgctgt tgcgcccttc tctgccttca cctcctggga cgtcccgacg
     2401 acggcgttga gcagcagaac ggcaaagatg aagatggcat cggtgagatc gacgaagatg
     2461 atagagatga tcccggctgc aaggagaacg tagatgagcg ggctcttgaa ctggcggagg
     2521 aagacctcaa atacagtcgg tgggcgcttc ttagggaggg cgttctcgcc gaagacctct
     2581 cttcgccggg caacctcctt ctccgtgagg ccatctgtcg gtgattcgag cagtcgctct
     2641 acctcatcca cctggagggc gtgccaggcg gtcgtccggc actcatcggg agagaggttt
     2701 ttatacattc tactacctct ttggtatgcg ttggagattc aatgactgcg aggatgcccg
     2761 gccgtcctac cgtccctgaa ggtatgatct ctctcaagac tacataaaaa ctccagtctg
     2821 cgctcaccgg gggag
//
LOCUS       DAOSZX010000133         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_27711_length_2825_cov_19.424910, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000133 DAOSZX010000000
VERSION     DAOSZX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27711_length_2825_cov_19.424910"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1739
                     /locus_tag="PK196_07980"
     CDS             204..1739
                     /locus_tag="PK196_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HON41390.1"
                     /translation="MSKKQGKKGKKTVSPAHGRLDDKGDLDAGITSPAPSGASPLDKV
                     VTAIRTSRYLQILIGLTFVGFILRFYNLGGNSLWLDEATTLTFARQSAGEIWASVAAG
                     EFNPPLFYWLEHGMILFGESEFVLRFLPALLGVLTIPAIYLIGKEFRDKNVGLLAAAL
                     LTFSPFHIFYSQEARAYAPMLFFFSLALLFYIKAYRSNETRSWVLFGIFSAIAFWMHF
                     YAFVPVAVLVLHALISRVGEIRRDLRNVRDIALSIIVFVLATLPLLVVTANLFLVRTS
                     SAPTYGIQGASVMYQTLLQISGFNDLIMVLFIFLFLVGVISTWREDRKGSLLLVFMMI
                     LPLAASLLLSSRMPMIPRYLIYLLPVYFIGIASSYPALYALVRDKRAIYLAIIGVFLI
                     STPFLATYYTTPQKNDWRGFSEELAGMTVEGDLIVVLPPYMSQPLDYYYSNATDGTIE
                     LGANTGEDLDAIREQYSDRRAFYVVTADILAVDPTGEALVWLDKNAEFVGQRMGIYLF
                     RSV"
     gene            1750..>2825
                     /locus_tag="PK196_07985"
     CDS             1750..>2825
                     /locus_tag="PK196_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON41391.1"
                     /translation="MSERYDLSVIIPTFNEEENIAAIIGAVNDVCSKSGIRAEILVVD
                     DNSNDRTIPIVQEITRRCENVRLIVRMNDHGLSQSVVDGFRAARSDILLVIDADFSHP
                     PELIPRFYEAIRGGADIAIGSRYTKGGDIEEWPLKRRIISLGATAFGRILFPEITDPV
                     SGFFAVRREVVDGAPLAPRGYKILMEVLGKGRWRSFVEIPFVFRDRAEGESKLRLETM
                     LDYLRQCCGIIWFSVTYRTGPVWTEWTKILRFGIVGLSGIFVNMGLLYVLTEVAGLYY
                     LVSAAIAIETSIVNNFIWNDLWTFNSGGRLRFTRRLHRFVSFQVVSMGGLAINMLILY
                     LLVEVGGIYYLIANLAGIFAAFAW"
BASE COUNT          590 a          771 c          787 g          677 t
ORIGIN      
        1 cacccggcag aggcgaggat gatgaccagt agaaccgcag ctgctataaa aggtatatgg
       61 ccccagctca tcgcaaaggg gttgcgcagc agggtatata tgtctttatc aatcgtcccg
      121 cggatgagag ggcgctctta ttcccctggc gaacgcaatt agcaatgcat atctgcgccc
      181 ggtgtcaagt tttgttgata aagatgagca aaaagcaggg gaagaaagga aagaaaaccg
      241 taagcccggc acacgggaga ctggacgata aaggtgatct cgatgccgga atcacctcac
      301 ctgcaccctc aggcgcctct ccgcttgata aggtcgtgac ggcgatcagg acgtccaggt
      361 atctccagat cctcatcggg ctgacgtttg tagggtttat actcaggttc tataaccttg
      421 gtgggaactc cctctggctc gacgaagcca caaccctcac gttcgcccgg cagtcggcgg
      481 gtgagatctg ggcaagcgtc gctgctggag agttcaaccc gccactattc tactggctgg
      541 agcacggcat gatcctcttt ggagagagcg agtttgtgct caggtttcta ccagcgctcc
      601 tcggcgtcct gacgatcccc gccatctacc taatcgggaa ggagttccgc gataaaaacg
      661 tcggactcct tgcagcagcg ctcctcacgt tctcaccctt ccatatcttc tactcgcagg
      721 aggcccgggc atacgcgccg atgctcttct tcttctccct cgcgctgctt ttctacataa
      781 aggcataccg gtcgaacgag acacgttcct gggttctctt cgggatcttc tcagcgatcg
      841 ctttctggat gcacttctac gcttttgtgc cagtcgcagt acttgtcctg cacgcgctga
      901 tcagccgggt gggagagatc aggagagatc tccggaacgt acgagatata gcactctcga
      961 tcattgtgtt cgtcttagct acgcttcctc tgctcgtcgt tacggccaac ctattcctgg
     1021 tgagaacatc atcagccccg acctacggca ttcagggtgc cagcgttatg tatcaaaccc
     1081 tgctccagat atcgggattc aacgacctga tcatggtgct gttcatcttt ctcttcctgg
     1141 tcggtgtcat ctcaacatgg cgcgaggaca gaaagggatc gctcctgctc gttttcatga
     1201 tgatcctccc gctggcagca agcctcctcc tctcctcaag gatgccgatg atcccgcgtt
     1261 acctcatcta ccttctgccg gtctacttca tcggcatagc atcatcgtat ccagcgctct
     1321 acgctcttgt tcgggataag cgagcaatat accttgcaat cattggtgtg ttcctgatca
     1381 gcacgccgtt ccttgcaacc tactacacga caccgcagaa gaacgactgg cgtgggtttt
     1441 ccgaggaact cgcgggtatg acggtagagg gcgatctcat agtagtgctg ccgccttaca
     1501 tgtcgcagcc gctcgactac tactacagca acgccaccga tgggacgatc gaactcggag
     1561 cgaataccgg ggaagacctc gatgcaatcc gggaacaata ttccgaccgg cgtgcgttct
     1621 acgtggtaac ggctgacata ctcgccgtcg accccacggg ggaggctctg gtctggcttg
     1681 ataagaatgc agagttcgtc gggcagcgca tggggatata cctcttcagg tcagtctaac
     1741 gaggtcatca tgagcgaacg ctatgacctt tcggtgatca tcccaacctt taacgaggag
     1801 gagaatatcg ccgccattat cggtgctgtc aacgatgtct gttcgaagag cggaatacgg
     1861 gccgagatcc tggtggtgga tgacaactca aatgaccgca ccatccctat cgtgcaggag
     1921 atcaccaggc ggtgcgagaa tgtgcgcctg atagtgcgga tgaacgacca tggcctatcc
     1981 cagtcggtcg tagatgggtt cagggcagct cggtcggata tcctcctggt catcgacgct
     2041 gacttttccc acccgccaga acttatccca aggttttacg aggctatacg ggggggtgcg
     2101 gatatagcca tcgggagcag gtacacgaag ggtggtgata tcgaggaatg gccactcaaa
     2161 cgccggatta tatctctggg agccacggca tttgggcgta tactttttcc ggagatcacc
     2221 gatcccgtga gcggtttttt tgccgtgcgc cgagaggtcg tggatggagc ccctctcgca
     2281 ccgaggggtt acaagatcct gatggaggtc ctcggcaagg gccggtggcg ctcatttgtc
     2341 gagatcccat ttgtcttcag agaccgcgcg gagggggaga gtaaactgcg gctggagacg
     2401 atgcttgatt acctccggca gtgctgcggc atcatctggt tttcggtcac ataccgcacc
     2461 ggtccagtct ggacggagtg gacgaagatt ctcaggttcg ggattgtggg gctttcgggt
     2521 atctttgtca acatgggact tctttacgtt ctgacggagg ttgctggcct ctattacctc
     2581 gtctcagcgg cgatcgcgat cgagacctcg atcgtgaaca actttatatg gaacgatctc
     2641 tggacgttta attctggagg gaggctcagg tttacgagga ggctccaccg gtttgtctcg
     2701 ttccaggtgg tctcgatggg cgggcttgcc atcaatatgc tgatactcta cctccttgta
     2761 gaggttggag ggatttacta cctcatcgcg aaccttgccg ggatctttgc agcgtttgcc
     2821 tggaa
//
LOCUS       DAOSZX010000134         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_27856_length_2814_cov_36.355926, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000134 DAOSZX010000000
VERSION     DAOSZX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27856_length_2814_cov_36.355926"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>2814
                     /locus_tag="PK196_07990"
     CDS             24..>2814
                     /locus_tag="PK196_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HON41392.1"
                     /translation="MEVRAIGNGAVHSGYFNDIDALAAAVEVMETVPDVNGIYITLNT
                     INPALLARRANRIKRTGGKDATTADGDIIRRRWLPVDIDPRRPSGVSSTDAEHQAALD
                     MRDRIADWLAEQGFPEPVRADSGNGAHLLYPIDLPNDDDATSLVKDVLSTLDALFSNG
                     DVNIDAANFNAARIWKIYGTLTRKGDNTPERPHRRARIDSIPAGLVEVPVERLRHIAD
                     ILPREDPAATTTGTARRATGPGIDLGAWLADHGIAVRSTKPWRGGTLYVLDECPFSGA
                     HRDGAYAIQFASGAIYAGCHHAGCGGGSQRWPELRAMYEPETDREKGTHATTTTGRPT
                     PTAGASIPTSSQTTTTAGLPAADYHLTDAGNSERLIRQFGDDIRYCAPLKQWFVWDGR
                     RWAPDEAHMMLDLATRTAKAIFLEAADADTIDQQREIARWAIRSESLAGRHAMIDGAV
                     FMVPVQPDELDAHPNLFNCVNGTLDLDTMEFREHRREDLLTKIAGVRYDPTAKCPLWI
                     EHLHMIFGGDEDVIAAFQQVVGYSLLQRNPEQLAFILWGSGKNGKSETLKPLAKIMGD
                     YAVNIEAETLMASRHGDGGRARPDVLRLRGARFVTVTEPGQNDVLSEPLIKSVTGDHA
                     VTARPLYGNPVEFRPGAKIFIGTNHLPKIRGMDEGIWRRIWLFPFTVTIPPERRIRDY
                     GDILFEREGSGILNWMIEGLRQYLARGELVQPEAVKRATQEYRISSNAIGRYLVEWCV
                     VDPREQVGKNDLYEGYREWCETVGRKPLSKTAFGKIMKTMFEDTREGEKGLRYWVGIR
                     RKTKSEIENDAQTFEVQEQLQPPEDSELGETDTTDTSYQTFSCTSSRGKVWENVSVVS
                     VPSSPPSDEPQGGVSPFLPTDEPAKSQMAYSPDPCDYVYLGRTEYNQQCAACGVRGVT
                     YRERPQAFNVKRGTAG"
BASE COUNT          654 a          808 c          880 g          472 t
ORIGIN      
        1 tcacccgtgg cagcggcggg gtagtggagg tccgggcgat aggcaacggc gcggtccatt
       61 cggggtactt caacgacatc gacgccctcg cggcagcggt cgaggtgatg gaaacggtcc
      121 cggacgttaa cgggatctat atcaccctga acaccatcaa cccggcacta ctcgcccggc
      181 gggcaaacag gatcaaaaga actggtggaa aagacgctac caccgcggac ggcgacatca
      241 tcaggcgtcg atggctcccg gtcgacatcg acccgagacg gccgagcggg gtatcatcta
      301 ccgatgcaga gcaccaggcg gcgttagata tgcgagaccg gatcgcggac tggctcgcag
      361 aacaggggtt tcccgaaccc gtgcgggcgg actcagggaa cggggcacac ctcctctatc
      421 ctatcgacct gccgaacgac gacgacgcta cgtcgttggt gaaagacgtt ctatcgacac
      481 tcgacgccct cttctccaac ggcgatgtga atatcgatgc cgcaaacttc aacgccgccc
      541 ggatctggaa aatttacggc acactgacac gaaaaggcga caacacacca gaacgaccac
      601 atcgacgggc gaggatcgac tcgatacctg cgggacttgt ggaggttccg gtggaacgac
      661 tacggcacat cgcggacatc ctcccgcgcg aagacccggc agcgacgacg accgggacgg
      721 cgcgacgagc taccggtccc gggatcgacc tgggggcgtg gctcgctgac cacgggatcg
      781 cggtccggag cacaaaaccg tggcgtggag gcacactata cgtgctcgac gagtgcccgt
      841 tttctggtgc acacagggac ggcgcgtatg cgatccagtt cgcaagtggc gctatctatg
      901 cagggtgtca tcatgccggg tgcggcggtg gatcgcaacg atggcccgaa ctgcgggcga
      961 tgtatgagcc cgagacggac cgcgagaaag gcacacacgc caccactacc accgggcggc
     1021 ctacgccgac ggccggggca tcgataccga cctcctcaca gacgacaacg actgccggtc
     1081 tgcctgccgc cgactatcac ctgacagacg ccggaaactc tgaacgtctg atccggcaat
     1141 tcggcgacga catccggtat tgtgccccgt tgaaacagtg gtttgtgtgg gacggccggc
     1201 ggtgggcacc cgacgaggca catatgatgt tagacctcgc gaccaggacg gcgaaggcga
     1261 tcttccttga ggccgccgat gccgacacaa tagaccaaca gagagagatc gcgcggtggg
     1321 ctatccggtc ggagtcgctc gccggccggc atgctatgat cgatggcgcg gtcttcatgg
     1381 tcccggtcca gccggacgaa ctggatgcac acccgaacct gtttaactgc gtgaacggga
     1441 cgctcgacct cgacacgatg gagtttcgag aacatcggcg cgaggatctc ctgacaaaga
     1501 tcgcgggcgt gcgatacgat ccaactgcga agtgtccgct atggatcgaa cacctgcaca
     1561 tgatcttcgg tggtgatgag gatgttatcg cggcgtttca acaggtggtt ggatactcgc
     1621 tccttcagag gaaccccgaa caactggcat ttatcctctg gggatccgga aagaacggga
     1681 aaagtgagac acttaaaccg cttgccaaga tcatgggcga ctacgccgtg aacattgagg
     1741 cggagaccct gatggcatca cggcacggcg acggggggcg ggcgcgaccc gatgtactca
     1801 ggttacgcgg tgcccgtttt gtgaccgtta ccgaaccggg acaaaacgat gtgctctccg
     1861 agccgctgat aaagtcggtg accggcgatc acgcggtgac tgcccggccg ttgtatggta
     1921 acccggtgga gtttaggccg ggcgcaaaga tcttcatcgg cacaaaccac ctgccgaaga
     1981 tacgcgggat ggacgagggt atctggcgac ggatctggtt gtttccattc acggttacga
     2041 tcccgccgga gaggcggatc cgcgactacg gcgacatcct ctttgaacgg gagggatccg
     2101 ggatcctgaa ctggatgatc gaaggccttc ggcaatatct ggcacggggc gaactcgtcc
     2161 agcccgaggc ggtgaaacga gcaacacagg agtaccggat ctcgtcgaac gcgatcggcc
     2221 gttacctggt ggagtggtgt gtcgttgatc cgcgggaaca ggtggggaaa aatgatctct
     2281 acgagggata ccgcgaatgg tgcgagaccg tcggccggaa accgctatcg aaaactgcct
     2341 tcggcaagat catgaaaact atgtttgaag acaccagaga gggggaaaag ggattaagat
     2401 attgggtagg aattcggcga aagacaaaat ccgaaattga gaacgacgca caaacctttg
     2461 aagttcaaga acagctccaa ccgcctgagg acagcgaact gggagagact gacacgactg
     2521 acacgtcata ccaaactttt agctgtacgt cctcgcgtgg gaaagtttgg gaaaacgtgt
     2581 cagtcgtgtc agtcccctca tcccccccca gtgacgaacc ccaaggcggc gtgtcgccct
     2641 tcctgccgac cgacgaaccg gcaaaatcac agatggcgta ctcaccagac ccctgcgact
     2701 acgtctacct gggacgcaca gagtataacc aacagtgtgc ggcatgcggc gtgcgtggcg
     2761 tgacctatcg cgaaaggccg caggccttca acgtaaaacg ggggacggcg ggtc
//
LOCUS       DAOSZX010000135         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_28384_length_2770_cov_13.029834, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000135 DAOSZX010000000
VERSION     DAOSZX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28384_length_2770_cov_13.029834"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..334
                     /locus_tag="PK196_07995"
     CDS             134..334
                     /locus_tag="PK196_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41393.1"
                     /translation="MLVKMPEKTTCIPARKRASAQTAFAIWKGGVDARPHEAASGHQQ
                     DPGEGVRDLEEVAGVIRVPMTI"
     gene            520..2250
                     /locus_tag="PK196_08000"
     CDS             520..2250
                     /locus_tag="PK196_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HON41394.1"
                     /translation="MRESLILRVAAVLLIVLTGIAPAAALDNLTADPVRIEEMTHDLC
                     TFERSSESKDRTAAATYIAAAMEERGLSVSTGRFAYTNCYFDPPLALSSNIIGVREGE
                     TDRIVIISAHYDTVHGTPGADDNAAGVATMLEVARILNATPLNQTVYFIAFGGEETGL
                     DGSRRWLADNQDIHGRTVVAINLDCIASGDRLLITALPQHRGILDALPPSACVEETPA
                     QLLDAARGDEISFRAAGIPALRIYERDSHAIIHTPDDRPERLNYTLAAECAGIVAGTV
                     VGLDAGGDEPEIDLSIENGTVFFTTPNGAPVEVIVDGTSLGAVPSGSVTLPGGVHTVE
                     AVTYGPTGAKAVATATGEGVGIVPPAVSGSAVTIPWGDEIEPGEDIPTQLALSAAVPL
                     SYRIDRPEEVLRVDGYFDGILIRDLENGLAVIPAPGQHSFTVAAYGADSSVLGVDRAD
                     FLLLQYGKVDIDGGLLEVDETGGIACSASARTHTRTYTPGERYWVVVGCDYRDTAEVF
                     LQMAEFRVDGPGGRKYECRFFDMPVLNDTRQSEIVLLLDPEGPGTYHWTISCSEGGHR
                     AAETGSLVLR"
     gene            2395..>2770
                     /locus_tag="PK196_08005"
     CDS             2395..>2770
                     /locus_tag="PK196_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41395.1"
                     /translation="MPVLSQPTVSPPASVTAVTTLQSSILSHAAGSVSAPTCRGGGKA
                     DEFYRFSCLVEHAGLEDLASLEQIQDFAVVSHYPGHPVIPGDPAVCGFVIIGIAHAHQ
                     KKKRRWPQRARRQLPIIILFSYL"
BASE COUNT          528 a          889 c          851 g          502 t
ORIGIN      
        1 cgccggatgt tcataccctc aaaccccccg cagatggtgt tgtttgtgag gggccggctc
       61 ccgggcaacg gggagaggtg gcagaagccc cggaaacagt tattcctgcc cctcccccct
      121 gttcaggagc accatgctcg tgaagatgcc ggagaagaca acctgcatcc cggcaaggaa
      181 gagggccagc gcccagacgg cgtttgcgat ctggaagggt ggagtcgatg ccaggcccca
      241 cgaggccgcc agcggccatc agcaggatcc cggcgagggg gtccgggatc tcgaggaagt
      301 tgcaggtgtc atccgcgtcc cgatgacgat atagcgcccc ccgggcacca gcacgcggag
      361 aggtgcgtcc ggcgctgtac cgaacctcta tcataaaggc atccgttacc gcctgcatcc
      421 ccggcggcca gaagagttga ctcttgacac agaaattagt tatagcgcga tgacgacctt
      481 ttcaatcggg aggccgggca cctaaaacag gatagaacca tgagagaatc attaatactg
      541 agagtcgccg ccgtgctcct gatcgtgctc accgggatcg caccggcggc cgccctcgac
      601 aacctcacgg ccgatcctgt acggatcgag gagatgaccc acgacctctg cacgtttgag
      661 cggtcttcgg agtcaaaaga tcggacggcg gcggcgacct atatcgccgc ggcgatggag
      721 gagcgcgggc tttcggtcag caccgggagg ttcgcataca ccaactgtta ctttgatccg
      781 ccgcttgccc tctcctcaaa catcatcggc gtcagggagg gggagaccga ccggatcgtt
      841 atcatctccg cgcactacga caccgttcac gggacgcccg gcgccgacga caacgccgcg
      901 ggcgtcgcga cgatgcttga ggtcgcccgg atcctcaatg ccacccccct gaaccagacg
      961 gtctacttca tcgccttcgg cggcgaggag accgggcttg acgggagcag gcggtggctc
     1021 gccgacaacc aggacatcca tggccggacc gtcgtcgcga tcaacctcga ctgtatcgcc
     1081 tccggggacc ggctgctgat aacagcgctc ccccagcacc gggggatcct cgatgcactc
     1141 cccccatcgg cctgcgtcga ggagacgccg gcacagctgc ttgacgctgc tcggggggat
     1201 gaaatctcct tccgtgccgc cggtatcccg gcgctccgca tctacgagcg cgacagtcac
     1261 gcgatcatcc atacccccga cgaccggccg gagaggctca actacacact cgccgctgag
     1321 tgtgcaggaa tcgtcgccgg gacggttgtg ggacttgacg cgggcggtga cgagcctgag
     1381 atcgatcttt cgatcgagaa cggaacggtt ttctttacaa ctccaaatgg cgcaccggtc
     1441 gaggtgatag tcgacggcac cagcctcggg gcagtcccgt ccgggtcggt caccctgccg
     1501 ggaggtgtgc acaccgtaga agcagtcacc tacggcccca ccggggcgaa agcggtcgcg
     1561 acggccacgg gagagggggt agggatcgtg ccgccagcgg tatcgggaag cgccgtcacc
     1621 attccctggg gggacgaaat cgaaccgggt gaagacattc caacccaact tgccctttca
     1681 gccgccgtcc cgctctccta ccgcatcgac cggcctgaag aggttctccg ggtcgatggt
     1741 tactttgacg ggatcctgat ccgggatctt gagaacggcc tggcggtgat ccctgccccc
     1801 gggcagcact ccttcacggt cgcggcgtac ggtgccgaca gctcggttct cggggttgac
     1861 cgggctgatt tcctcctcct gcagtacggg aaggttgaca tcgacggagg tctcctcgag
     1921 gtcgacgaga ccggcgggat agcctgctcc gcatcggccc gcacccacac ccgcacatac
     1981 actcccggtg agcgctactg ggtcgtggtg gggtgcgact accgggacac cgcggaggtg
     2041 ttcctccaga tggcggagtt ccgggtcgac ggccccggcg gccggaagta tgaatgccgc
     2101 ttctttgaca tgccagtcct caacgacacc cggcaatctg agatcgtgct cttgctggac
     2161 ccggaggggc cgggcaccta ccactggacg atctcctgca gcgagggcgg ccacagggcc
     2221 gcggagaccg ggagcctcgt cctccggtga agcccttggt tatcaggccc gttgtgagtg
     2281 ttgcaatccg gtttccggca acgctccggg ttatagtcgc ggaaactgcg gtgacatcaa
     2341 ccagttttcg cccattcagg cagttggtct atctttctct cccagtcggt ggtattgccg
     2401 gtgctctccc aaccgacggt ctcgccgccg gcatcggtga cggcggtaac cactctacag
     2461 tcatcgatac tctcccatgc tgcaggatcg gtctccgccc ccacatgccg gggaggagga
     2521 aaggcagatg aattttaccg attctcctgt ctcgttgaac acgccggcct tgaagatctg
     2581 gcctccctcg aacagatcca ggacttcgct gtcgtttcgc attatccggg tcatccggtc
     2641 atacccggag acccggccgt gtgcggcttc gtcatcatcg gcatagccca tgctcaccag
     2701 aagaaaaaac ggagatggcc gcagagagca cgacggcaat tgccaataat aatcctgttt
     2761 tcttatttga
//
LOCUS       DAOSZX010000136         2767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_28417_length_2767_cov_18.939897, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000136 DAOSZX010000000
VERSION     DAOSZX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28417_length_2767_cov_18.939897"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK196_08010"
     CDS             complement(<1..890)
                     /locus_tag="PK196_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON41396.1"
                     /translation="MNQHIFVDRAVEQQFLTDRYRSPIPEFLILYGRRRVGKTTLLAR
                     FLQTVPGFYFLASEEGTARNVQDFSRYAGEYLNDPDFERSRYPDWEAVIKALASHRNF
                     SPPPGQKVVIVIDEFPYLIARDRATPSVFQKVWDTVLSREPVMLVISGSSVSTMETEV
                     LGYTSPLYGRRTGQWHLEPLSYPYLRQFLPYDEEDLVMTWCVIGGIPAYLRLFRPDRT
                     FWENVEEQILQKGAYLYSEAEILMQYEFREAGNYMAILRALAAGYTTLGRLCQETGLD
                     KGLVSKYLAVLSRMHLIDDEV"
     gene            1174..1569
                     /locus_tag="PK196_08015"
     CDS             1174..1569
                     /locus_tag="PK196_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41397.1"
                     /translation="MGGNRFVCGNGAGRTTAYAELLLEHGGFPAAVVETEGGLYWIPR
                     LRVRDGRWVSAMAFTGRCEAFSSCGCIALLGALVFWRAWEKRCWKGGVVESGDLFLAG
                     FRREKSGVQRQGGDSSTDAAAGEWVTASR"
     gene            1606..2652
                     /locus_tag="PK196_08020"
     CDS             1606..2652
                     /locus_tag="PK196_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41398.1"
                     /translation="MILEEVVAFSEEVDAVEFVSFLKSKGCRARIEVRGNANAEELLE
                     GALDDLIAWFEEAAAQSGEEPPELSDDTRWFLDAAEKYRRQRDQIAALLEGHKPGDIL
                     YTNDDLKRSQEELFHKMAEKVREWIEGNKDAGEELDESALFEALFGDHDLLPEDAPLF
                     VLHTVLMRNGILEETPEGCRLTREIPTGQVVLEISTDNLHDLDVDLPEDAGLKKKAVY
                     WIDPVYQVVIDPAIHLVCDTDEVLTALEGTTVDIESATTLEENLRVKEFIIATLLESV
                     KRKPGITVDELSGPIGVEGVNEDGESIRIEIAIDRRMTSIIVAELRKLGMLTGSNQKI
                     KIGRTPGGKKRQNQ"
BASE COUNT          575 a          760 c          905 g          527 t
ORIGIN      
        1 acctcgtcgt cgatcaggtg catacgcgag aggacggcaa ggtacttgga gaccagcccc
       61 ttatcaagcc cggtctcctg acagaggcgt ccaagggtgg tgtagcccgc agcgagagcg
      121 cggaggatgg ccatgtagtt ccctgcctcg cggaactcat actgcatcag gatctcggct
      181 tcggagtaga ggtatgcccc cttctggagg atctgctcct ccacgttctc ccagaaagtc
      241 cgatcggggc gaaacagacg gagataggca gggatcccac cgatcacgca ccatgtcatg
      301 acgagatcct cctcatcgta ggggagaaat tggcgaaggt agggatagga aagaggctcg
      361 aggtgccact gccccgtcct tctgccgtag agcgggcttg tgtaccccag cacctcagtc
      421 tccatcgtgc tgactgatga acctgatatg acgagcatca ccggctctct ggagagcacc
      481 gtatcccaca ccttctgaaa gacggaaggc gtggcccggt cgcgtgcgat gaggtagggg
      541 aactcatcga tgacgatcac cactttctgc cccgggggag gggagaaatt ccggtgactg
      601 gccagcgcct ttatgacggc ctcccagtcg ggatacctgc tccgctcgaa gtccggatcg
      661 ttcaggtact cccctgcata gcgggagaaa tcctggacat tccttgctgt cccttcttct
      721 gacgccagga agtaaaagcc cgggacagtc tgcaggaacc gggcaagaag cgtcgtcttt
      781 cccacgcgcc gcctcccgta caggatcagg aactccggaa tcggggaacg gtatcgatcg
      841 gtgaggaact gctgttcaac tgcccggtct acgaagatat gttgattcat aagtatacta
      901 ctttgtagtc aacaaacact tacggtgatc ggtaagcgcc ggtggtccgg ccggtcatgg
      961 cgccgctgca aaggcaggca gccccttgcc ggggttgatg gctgtaaccg ggcggccttc
     1021 gcggcgatgt cgcttcgctg gaggaggcag agcggctttc cctccaggcg cctggtagaa
     1081 cccggggcag ccgatgcccg cggggctgag cggatggtag tgccctctgg tcttgaccta
     1141 ctccctgccc acggccgaga ggtgcgctgc gtggtgggtg gcaatcgctt tgtttgcggc
     1201 aatggcgcgg ggcgcaccac cgcatacgcc gaactcctcc tcgaacacgg cggtttcccg
     1261 gcagcagtgg tcgaaactga ggggggattg tactggatcc cccgtcttcg agtgagagat
     1321 gggcgatggg tctctgcaat ggcattcacg ggaagatgcg aggcattctc cagctgcggt
     1381 tgtatagcac tgctcggcgc tctggtgttc tggcgggcat gggagaagag atgctggaag
     1441 gggggggtgg ttgagagcgg tgatctcttt ctggccgggt tccgccggga gaagagcggc
     1501 gtgcagagac aagggggtga ttcctccacc gatgcagcgg ccggggagtg ggtcaccgcc
     1561 tccaggtgag atccttttta tctctaccag cgcccaccct atggtatgat cctcgaagag
     1621 gtcgtcgcct tcagtgaaga ggttgacgca gtggagtttg tcagttttct caagagcaaa
     1681 ggctgccgcg ccagaataga ggtgcggggg aacgccaacg ccgaggagtt gctggaggga
     1741 gcgctcgatg acctgatagc ctggttcgag gaggctgccg cgcagagcgg agaagagccg
     1801 ccagagttat cggatgacac ccggtggttc ctggatgccg cggagaagta ccggcgccag
     1861 cgcgaccaga tcgcggcgct gctggagggg cataagccgg gggatatcct ctacaccaac
     1921 gatgacctca agcggtcgca ggaggagctg ttccacaaga tggcggagaa ggtccgggaa
     1981 tggattgagg gaaacaaaga tgccggtgaa gaactggatg agtcggcgct gtttgaggcc
     2041 ctcttcggtg accacgatct cctcccggag gacgctcccc tcttcgtgct ccacacggtg
     2101 ttgatgagaa acggtatctt agaggagacg cctgaaggct gccggctgac ccgtgagatc
     2161 cccaccgggc aggttgtgtt ggagatctcc accgataacc tgcatgatct cgatgtcgac
     2221 ctcccggagg acgccgggct gaagaaaaag gccgtctact ggatcgatcc cgtctaccag
     2281 gttgttatcg accccgcgat ccatcttgtc tgcgatacag acgaggtgct caccgccctg
     2341 gagggcacca ccgtggatat cgagtctgcg accactctgg aggaaaacct ccgggtgaag
     2401 gagttcatca tcgcgacact ccttgagtcg gtgaagcgga agccgggcat cacggtggac
     2461 gaactttcag gccctattgg agttgaaggg gtcaatgagg acggagagtc gataaggatt
     2521 gagattgcca ttgaccggcg catgacctcg attatcgtcg ccgaactgcg caaactgggc
     2581 atgctcacag ggagcaatca gaagatcaag atcgggagga cgccaggcgg caagaagaga
     2641 cagaatcagt gaggcggggg ggatgtgcgc gagagggcgg cgggctcccc catcccatct
     2701 ctccagcacc tcacccggaa acgtccgctg tctcagaaga cccgtggcac cgtgtagatg
     2761 gataatc
//
LOCUS       DAOSZX010000137         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_28568_length_2755_cov_17.728519, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000137 DAOSZX010000000
VERSION     DAOSZX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28568_length_2755_cov_17.728519"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK196_08025"
     CDS             <1..180
                     /locus_tag="PK196_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HON41399.1"
                     /translation="VEFDRITEIRGRVYRGVLFGRPVSLIPTLHPAAALYNPGYREAL
                     EEDFRVIERELAAAD"
     gene            204..635
                     /locus_tag="PK196_08030"
     CDS             204..635
                     /locus_tag="PK196_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON41400.1"
                     /translation="MVKERSCGAVVVRRDTEPQYLILRYGAGHWDLVKGHGVGDETEE
                     ETVLRELEEETGITQTRFIPGFRQEVHYFFQRRGRTVYKEVVYYLIETPEKEVRLSDE
                     HIAYLWLPYDRVMRMITFANSRGVVEAAHDHLTRLWNLNDG"
     gene            complement(707..>2755)
                     /locus_tag="PK196_08035"
     CDS             complement(707..>2755)
                     /locus_tag="PK196_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HON41401.1"
                     /translation="RMLTEFEGYQLLESWGVPVPPHTLVSSAMEAREAAGRIGYPVVM
                     KVISPQIVHKSDVGGVITNIETPEAAEEAFRTIMRNSAANAPEATITGIIVERQMPGG
                     VEVLIGGKTDPTFGKVITFGLGGKLVELLKDVSIRVLPVGEDEIRSMIREIRGYSLIH
                     GYRGEPPKDEDALVNLIAAMARKFVEIPEIREFDLNPVILYERGASVVDARIIVGEAT
                     GEEAPHRPGTDLETPSDIFYPDSIAVIGASASPNKVGYSVLRNLLSFPGKLYPVNPSR
                     TEIFGRKAYPSVKEIPGPVDWAVIAVPARIIPEVMEECGEKGVRLAIIVAAGFREIGD
                     EGAALEERVTAVARRHGIRIVGPNCLGVMMPHMGINATFDPVSPRPGDVAFISQSGAI
                     ITTIVDWSLPADFGFSTVISVGNQADLGFEHYLRFAEQDDMTRSITLYIEEILDGRRF
                     MEIVREITPRKPVVAVKSGSSRKGQATASSHTGSLAGSYEVYIAAFRQAGVIPARSLR
                     EAFNLAELLASEGYPRGNRAIAVTSAGGFAVLASDYAEAYGVDMVEIPDDVLHELNAF
                     LPPYWNHTNPMDILGDADATRFAALFDVLIRHQEFWDIAFAIAVPTTTVDPTHVANEI
                     VRFSRNTSKMVVGCMLGGDSIKSGLRILRGARIPNFEELEDAFKAVGNILDGRMVRER
                     "
BASE COUNT          544 a          834 c          817 g          560 t
ORIGIN      
        1 gtcgagttcg accggatcac agagatccgg ggaagggtat accgtggcgt tctcttcggc
       61 cgcccggtct ccctcatccc caccctccac cccgccgccg cgctatacaa ccccggttac
      121 cgggaggcgc tggaagagga tttcagggtc atcgaacgcg aacttgcagc ggcagattga
      181 ccacgacgaa ggattgaata ataatggtaa aagagcgctc atgcggtgcg gtcgtcgtcc
      241 gccgggatac agaaccccag tatctcatac tgcgctacgg cgcgggtcac tgggacctgg
      301 tgaagggtca tggcgtaggc gatgagaccg aggaggagac ggtgctccgc gaactggagg
      361 aggagaccgg gatcacccag accaggttca taccggggtt ccgccaggag gtacactact
      421 tcttccagcg ccgtgggcgg acggtctaca aggaggtagt ctactacctt atcgagacac
      481 ctgagaagga ggtgaggctc tcggatgagc atatcgccta tttgtggctc ccgtatgacc
      541 gggttatgcg gatgatcacg tttgcaaact caaggggtgt tgtggaggcc gcacacgatc
      601 acctgaccag gctctggaat ctaaacgacg gttgaccctt gcctgcagat ccaaaaaccc
      661 cggtctaaaa aaaagcggaa ccgggatgat ggtgggcggc agcccatcac ctctctctga
      721 ccatcctgcc gtcgagtatg ttcccgaccg ccttgaacgc gtcctcaagt tcttcgaagt
      781 tcggaatgcg ggcacccctg aggatgcgca gtccactctt gatactgtcg ccgccgagca
      841 tgcagccgac caccatcttg ctggtgtttc gggaaaacct gacgatctcg tttgcgacat
      901 gtgtcggatc gacagtcgtg gtcgggacgg cgatcgcaaa cgcgatgtcc cagaactcct
      961 gatggcgtat cagcacatcg aagagagcgg caaaccgtgt ggcgtcggcg tctccaagga
     1021 tatccatggg gtttgtgtgg ttccagtacg gcggcaggaa ggcgttgagt tcatgtagca
     1081 cgtcgtccgg gatctcgacc atgtccaccc cgtaggcctc cgcgtaatcg gaggcgagaa
     1141 cggcgaaccc cccggcactg gtgacagcga tggcacggtt cccccgcggg taaccctctg
     1201 atgccagtag ttcggccagg ttgaaggctt cccgcaggct tctggcgggg atcacgcccg
     1261 cctggcgaaa cgccgcgatg tagacctcat aactccctgc aagcgacccc gtgtgggacg
     1321 acgcagtggc ctgccccttt ctggatgacc cggacttcac cgccaccacc ggctttctcg
     1381 gggtgatctc gcgaacgatc tccatgaatc tgcgcccatc caggatctcc tcaatgtaga
     1441 gggtgatcga gcgtgtcatg tcgtcctgct cggcaaaccg gaggtagtgt tcgaacccaa
     1501 gatccgcctg gttaccgacg ctgatgacag tcgagaaccc gaaatccgcc ggcaggctcc
     1561 agtcaacgat ggtggttatg atcgcgccgc tctgggagat gaaggccaca tcgccgggtc
     1621 gaggcgagac gggatcgaac gtggcgttga tccccatgtg tggcatcatg acaccgagac
     1681 agttcgggcc gacgatcctg ataccgtgcc tccgtgcaac ggctgtcacc ctctcttcga
     1741 gagcggcacc ctcatcaccg atctcgcgaa accccgcggc gacgatgatc gcgagccgga
     1801 cgcccttctc accacattct tccataacct ccgggatgat gcgggcggga acggcgatga
     1861 ccgcccagtc caccggcccc gggatctctt tcacggaggg gtaggccttt cgcccgaata
     1921 tctctgttct ggatgggttt acggggtaga gtttccccgg aaacgagagc aggtttcgaa
     1981 ggacggaata gccgaccttg ttagggcttg cggaagcccc gatcacggct atggaatccg
     2041 ggtagaagat atctgatggt gtctcgagat cggtgccagg gcggtggggg gcttcctctc
     2101 cggtcgcctc cccgacgatg atccgcgcgt ccacaacgct tgctcctctc tcatagagta
     2161 tgacggggtt gaggtcaaac tcccggatct cgggtatctc gacgaacttc cgggccattg
     2221 cagcgatgag gttgacaagc gcgtcctcgt cttttggggg ttcgccgcgg taaccgtgga
     2281 tgaggctgta gcccctgatc tcgcggatca tgctgcggat ctcgtcttcg ccgacaggaa
     2341 gcacccggat ggagacatct ttcagcagtt ccaccagttt cccgccgagg ccaaacgtga
     2401 tgaccttccc gaacgtcggg tcagtcttcc cgccgatcag cacctccacc ccgcctggca
     2461 tctgtctctc cacaatgatg ccggtgatcg tagcctcagg agcatttgcg gcgctgtttc
     2521 tcatgatcgt ccggaacgcc tcttcagcag cctcaggcgt ctcgatattg gtgataactc
     2581 ctcctacatc gctcttatgg acgatctgcg gggatatgac cttcattaca accgggtagc
     2641 ctatccgccc agcggcttcc cgtgcctcca tggcgctgct gaccagggtg tggggcggca
     2701 cgggtacccc ccatgattca agcaactggt aaccctcaaa ctcggttaac atcct
//
LOCUS       DAOSZX010000138         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_28657_length_2748_cov_21.921649, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000138 DAOSZX010000000
VERSION     DAOSZX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28657_length_2748_cov_21.921649"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..207)
                     /locus_tag="PK196_08040"
     tRNA            complement(133..207)
                     /locus_tag="PK196_08040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Arg,seq:cct)
     gene            278..1393
                     /locus_tag="PK196_08045"
     CDS             278..1393
                     /locus_tag="PK196_08045"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HON41402.1"
                     /translation="MDLVEAAEGRTRFFVPRQDPGLQFPPGNAPVFYNPRMELNRDTT
                     VLLLTLLRPRDYLDAMGASGVRGLRVAGEAGIPVTINDRSERAADLIRHNVKTSGLEI
                     EVTCQDANVLMSGRRFDAVDLDPFGTPAPFTDSAARSAGNYLFVTATDTAPLCGAHLP
                     AGMRRYFSRPRNTEYHAEVGLRTLLGFVVREVIRYDRGVEPLFCYAHEHFHRLHLRLR
                     SGAAAADRTLARIGYVMQCPGCLYRTEEAGILPEPADCPLCGARLVAVGPLWTGRIND
                     DQTLAAMQEALPSVTAGTAPRISRLLSTCRQELDTSSHYDHHVLAGSLRVSPAGIESV
                     IERLRSLGYSASRAHYSGTALKTDAPLPLLQEVISGG"
     gene            complement(1380..2495)
                     /locus_tag="PK196_08050"
     CDS             complement(1380..2495)
                     /locus_tag="PK196_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HON41403.1"
                     /translation="MNSLEEQIREIEDEIRKTPYNKATSKHIGRLKAKVAKIRDEAVT
                     RAMASAGGGEGYSVRKSGDATVVLVGFPSVGKSTLLNSLTGENLSETASYAFTTVSVI
                     PGLMEHRGAKIQILDIPGLIAGAAMGKGRGKEVIAVVRSADLILILVDVFNQQHTDVL
                     LRELHDAGIRINRERPDITIKKTGSGGIRLNTVGAVDLDIEEIRSILAENKIMNAEVL
                     IREGVTQDDFIDAMIGNRVYVPAFIAVNKVDLVDERMRQEIEKNLTERFGEPPIMVSA
                     QSGYHIEELRDAIFDNLRFMRVYLKPVGGPADLDEPLIIRSPATVEDVCNRLHRDFAD
                     KFRYAKVWGRSAKHDAQRVGLNHRLADGDILTIVTRR"
     gene            2673..2747
                     /locus_tag="PK196_08055"
     tRNA            2673..2747
                     /locus_tag="PK196_08055"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2709..2711,aa:Glu,seq:ctc)
BASE COUNT          505 a          886 c          819 g          538 t
ORIGIN      
        1 ccccgcccct gtcttgacgt gtcactgaaa acctctctcg ctggaaacac ccccacctct
       61 ctcctcttgc cgggacacca tctccccggg tgaggcaaaa tccataattc atgcagtatt
      121 ggagaaaagc agcgggcttg gggggatttg aacccccgac attcagctta ggaggctgac
      181 gccatatcct ggctaggcca caagcccacg cactggacta agagtgttgc ccatgagaag
      241 gtataagggt atcgagcatc cacgtttccc ggaaaatatg gatctcgttg aggctgccga
      301 agggagaacc cggttttttg tgccacggca ggacccgggc ctccagttcc cgccgggaaa
      361 cgccccggtc ttttacaacc cgcgcatgga gttgaaccgt gacaccaccg tcctgctgct
      421 cacactcctc cggccgcggg attacctcga cgccatgggg gcttcaggcg tccgcgggct
      481 ccgggtggcg ggcgaggccg ggataccggt gacgatcaac gaccggagcg agagagcggc
      541 cgacctgatc cggcataacg tcaagacctc cggcctggag atcgaggtca cctgccagga
      601 cgcaaacgtg cttatgagcg ggaggaggtt tgatgccgtc gacctcgacc ccttcggcac
      661 accggcgccc tttaccgact ccgccgcacg gagcgccggg aactacctct tcgtcaccgc
      721 caccgacacc gcaccgctct gcggggccca cctcccggcg gggatgcgcc gctacttctc
      781 ccggcccagg aacaccgagt accacgccga ggtaggtctc cggacgctgc tcgggtttgt
      841 cgtgcgcgag gtgatccgct acgaccgcgg cgtggaaccg ctcttctgtt acgcccacga
      901 gcacttccac cggctgcacc tccggctccg cagcggtgct gcagcggccg accggacgct
      961 ggcacggata ggctacgtga tgcagtgccc cggctgcctc taccggacgg aagaggccgg
     1021 gattctcccc gaacctgcag actgcccgct ctgcggcgcc cgcctcgtcg cggtcggccc
     1081 cctctggacg ggcaggatca acgacgacca gaccctcgcc gcgatgcagg aggccctgcc
     1141 gtcggtcaca gcggggacgg ccccccggat atcgaggctg ctctcaacct gccggcagga
     1201 actcgacaca tccagccact acgaccacca cgttctggca ggatcgctac gtgtctcccc
     1261 ggccgggatc gagagcgtga tcgagcggct caggtctctt ggctacagcg caagccgcgc
     1321 ccactactcg ggcaccgccc tgaaaacaga tgccccgctg cccctcctcc aggaggtcat
     1381 cagcggcggg tgactatggt caggatgtcg ccgtcagcga gccggtggtt gagcccgaca
     1441 cgctgggcgt cgtgtttggc ggatctgccc caaacctttg cgtaacggaa cttatccgca
     1501 aagtcgcggt ggagacggtt gcagacgtcc tcgaccgtcg ccgggctccg gatgatcagc
     1561 ggctcgtcca ggtcggcggg gccgccgacc ggtttcaggt agacgcgcat gaaccgcagg
     1621 ttatcaaaga tggcgtccct gagctcctcg atgtggtagc cgctctgcgc agagaccatg
     1681 atcgggggct caccgaacct ctcggtaagg ttcttctcga tctcctgccg catcctctcg
     1741 tcgaccaggt cgaccttgtt gaccgcgatg aacgccggga cgtagacgcg gtttccaatc
     1801 atagcatcga tgaaatcatc ctgggtgact ccttcacgga tcaggacctc ggcgttcatg
     1861 atcttgttct cggccaggat cgagcggatc tcctctatat cgaggtcaac ggcaccgacg
     1921 gtgttcagcc ttatcccgcc gctgccggtc ttcttgatgg ttatatcggg cctctcccgg
     1981 ttgatccgga tacctgcatc gtggagttcg cggagtagga cgtctgtgtg ctgctggtta
     2041 aagacgtcga ccagtatcag gatcaggtca gcgctccgca cgacagcgat gacctccttc
     2101 ccgcggcctt tccccatggc tgcgccggct atcagccccg ggatatccag gatctggatc
     2161 tttgcgcccc ggtgctccat caagcccggt atgacggaga ctgtcgtgaa ggcgtaggag
     2221 gccgtctcgc tcaggttctc accggtgaga ctgttgagca gcgtgctctt accgacagac
     2281 gggaaaccga ccaggacaac ggtggcgtct cccgatttcc tgacggagta gccttccccc
     2341 cctccggcgg aggccatagc ccgggtcaca gcctcgtcac ggatcttcgc gactttcgcc
     2401 ttgaggcggc cgatgtgttt ggaggtcgcc ttgttgtagg gagttttcct gatttcgtcc
     2461 tcgatctccc gtatctgctc ttcgagactg ttcattgctg ccggtatagg ttagatggga
     2521 agctacttat gggtgtctgt gccagaaatc gcgaagaaaa aagcccggat caggctcaga
     2581 tcgagtggtt gccgtcagaa atcgggacga gaatggcggg acaggtttaa ataccataac
     2641 agacaacgtt ttaaggcaca ttggtgcact gcgctgctat agtgtagacc ggccaatcat
     2701 gcaggactct cactcctgcg actggggttc gaatccccat agcagcat
//
LOCUS       DAOSZX010000139         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_28918_length_2728_cov_19.941264, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000139 DAOSZX010000000
VERSION     DAOSZX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28918_length_2728_cov_19.941264"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1587
                     /locus_tag="PK196_08060"
                     /pseudo
     CDS             268..1587
                     /locus_tag="PK196_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            1599..2213
                     /locus_tag="PK196_08065"
     CDS             1599..2213
                     /locus_tag="PK196_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HON41404.1"
                     /translation="MELDPFWKDALTEFGNIGAAHAATSLSQMLMSPVEMTVPEVMAI
                     DIADLHKYISNEISAMVVFQIQGEIADGGYIVVNMPRQTIIQLTNQMLGMTDTDREIN
                     EMDQSAAIEIGNIMISAFLDATAELLGIIMLPSPPALAIDMAHAAFESIVAQVATDVN
                     DVLIFNTELTSEAPPVYGSIYMLPNVELAGQLFRMLERLMEPLS"
     gene            2232..2723
                     /locus_tag="PK196_08070"
     CDS             2232..2723
                     /locus_tag="PK196_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HON41405.1"
                     /translation="MENNFFGEETAVILGLGEHRVGNYPMATIGLGSCIALILHDRRR
                     SVAGLAHIMLPESRGIADRPGKFADTAITVLLDEMESQGCTRNTISAFVVGGASMFVF
                     SANSLNIGERNTAAVKENLQKQRIRIEYEETGGKVGRSVYFWPEGKGCLIIKRADGTC
                     SRV"
BASE COUNT          595 a          724 c          890 g          519 t
ORIGIN      
        1 cgtcattttt gattatggcc gcgcgggggc ggcaagccgg gcggttggct cccgccctgg
       61 atagggggag cgtctccgct ccccctcccc gtcagcccct cccctacggg ggcgattctc
      121 cattgataca gtgtcggggg agtgcatcac ctcacccggt tcccgctgag aaaccgccga
      181 agatcctgtt tcctctctgt gcactgtatc cggggctatc gccagacact gcccgccccc
      241 tctggagggg gcgggaggag gccgcggagc gatgccgggg aggaggcgct tcggacgatt
      301 gcctccggca cagagatctc tctacttgca ctatctccgc tggaatcgta ttcggcggca
      361 gagagcctgg aagcgcggga gcccgcagac gtcccggaga tcggtgcggc gccggcgccg
      421 cagatgcaga aggcggcaag acccgataag gcccgggaga tcaagaacat ccgtgtcgat
      481 atcgagcggc ttgatcaaat gatgaacctg gtggaggatc tggtcatcaa ccgcggcagg
      541 ctcgagcaga tcgcgaggaa gcatggaatc aaggagtttg atgaagcgct gggcatgatc
      601 gggcgttcgg tctccgacct ccagaacctt atgatgggga tcaggatgat gccgcttgag
      661 cgcatcttca accgttttcc cagggttgtg cgggacgtag cgaaccatga cgggaaggag
      721 gttgagttca tagttgaggg cggcgagacc gaacttgaca ggagcatgat ggacggcctc
      781 tccgacccgc tgctgcacat cataaggaac gctgtgaacc acggcatcga gacgccggag
      841 gcccggcgag ccgcgggcaa acccgcaaag gggctggtaa agcttacggc ccgcagggac
      901 aaggacaacg tcatcataga cgtggaggac gatggtgcag gcatcgacca cgagaaactc
      961 aggaagaagg ccgttgaaaa agggtttctc gcacccgaga cggcggcgaa cgcgacgaag
     1021 gacgaactta ttgctctcct cttcgaacca gggttcagca cctccgaggt catcgacgac
     1081 atcagcggga ggggggtcgg gcttgacgtg gtcaagaaga cgatcgagtc gctcaagggc
     1141 acgatcaagg tcgagagcga gcctggcagg ggcacaaaat tcgagctggt tctgccgccg
     1201 acgatggcga tcatcgatgt catgatggtg cgcatcaacg gaaggcggtg tgccatcccg
     1261 atcagcaacg ttgtcgaggt ggcgcatgca agaccggagg cgatccaccg gatcggcaac
     1321 tccgatgcga tccttctgcg cgatcagacc ctccccctct accgcctgga ggatatgttc
     1381 ggccgggctg agaagggcga tacgctgatc gtgctgcaga atgatggcaa aaggtgctgt
     1441 atcgtcgtcg attatgttga cggtcagcag gaggtggtgg tgaaaccgct atcgaggatt
     1501 gcggggaact gccgcgggat cagcggtatt accataccgg gggacggtga tgttgtgccg
     1561 gtacttgatg taaatacaat gatttaggag aggttttcat ggaacttgat cccttttgga
     1621 aggacgcgct gacggagttc gggaacatcg gagcggcgca cgcggcgacg agtctgtcgc
     1681 agatgttgat gagcccggtt gagatgacgg tgccggaggt catggccatc gatatcgccg
     1741 accttcacaa gtatatcagc aacgagatat ctgcgatggt ggtctttcag attcagggag
     1801 agatcgcgga cggcgggtat atcgttgtca acatgccccg gcagacgatc atccagctta
     1861 caaaccagat gctcgggatg accgataccg atcgcgagat caacgagatg gaccagagcg
     1921 ccgcgatcga gatcgggaac atcatgatat cggcgttcct ggatgcgacc gcagaactgc
     1981 tcgggatcat catgctgccc tcgccaccgg cgctggccat cgatatggcg catgccgctt
     2041 ttgagtccat cgttgcccag gtggcaaccg atgtgaacga cgtcctgatc ttcaacaccg
     2101 agctcacgag cgaggccccc ccggtctacg ggagcatcta catgctcccg aacgtagaac
     2161 ttgccgggca gttgttccgg atgcttgaac ggctgatgga accactctcg tagacgccct
     2221 gcggaatata catggagaac aatttttttg gagaggagac ggcagtcatc ctggggctcg
     2281 gcgaacaccg tgttggaaac tatccgatgg cgacgatcgg gcttggctcc tgtatcgcgc
     2341 tgatactcca cgaccggcgg cgctcggttg cgggtctggc tcatatcatg cttcctgaaa
     2401 gccgcggaat cgccgatcgt ccaggcaagt ttgcggacac cgccatcact gtccttctcg
     2461 atgagatgga gagccagggg tgcaccagaa acacgatctc tgcgtttgtt gtcggcgggg
     2521 cgagcatgtt tgtattctcg gcaaactcgc tgaatatcgg ggagcgaaac actgctgcgg
     2581 ttaaggagaa cctgcagaaa cagaggataa ggattgagta cgaggagacg gggggaaagg
     2641 tcggtcggtc tgtctacttc tggccggaag ggaaaggctg cctcatcatc aagagggcgg
     2701 atgggacatg cagccgggta tgaaattc
//
LOCUS       DAOSZX010000140         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_29470_length_2685_cov_21.856274, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000140 DAOSZX010000000
VERSION     DAOSZX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29470_length_2685_cov_21.856274"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(501..573)
                     /locus_tag="PK196_08075"
     tRNA            complement(501..573)
                     /locus_tag="PK196_08075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(538..540),aa:Thr,seq:ggt)
     gene            complement(654..1379)
                     /locus_tag="PK196_08080"
     CDS             complement(654..1379)
                     /locus_tag="PK196_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HON41406.1"
                     /translation="MFGVSTYCLQHQPLDAALDTLAPITGCVEVMDGGLHSLETAEPL
                     ESHSFRYFIHAPFRGVNIASLLEPIRQASVDVLVQAFAVAAEVGADVVIHPGYYAWRE
                     EREESTRQFQKSLSELSAGADDLSVTFFVENMGGPDYFFLRSCDDLPLIDGIGLALDV
                     GHANLNGCLDCFLDHPAAHFHLHDNDGMRDTHSPVGTGTIDFAAVMEAVHRSGVIPIV
                     EVETLEGVLASIAALEAIDAGRR"
     gene            1501..2679
                     /locus_tag="PK196_08085"
     CDS             1501..2679
                     /locus_tag="PK196_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41407.1"
                     /translation="MADGFVPEVVVSNHLEKVKAEIFSYINSNARAIDDNFAAFFGNI
                     VTILDNAGIPIPDQVYDEFIDSIAYRVFDASKRAGDLKFLTRACEIACGMKRKKERKA
                     GVHLAAAVKLMKIGMPLAATAFLQPYRKFDAAVGCWCAYCYYTLYKEGSSEPGYSASE
                     RWNYLKTARQQMEELGQAQPGLNRLVKGELADDRWLIEPFWVMIFLATEWVPENRWLL
                     EIGITRAKQDKNEPALVKLLQLGFIRFPNDMLFYREAYHLKFEQGDLADALGLIRDLL
                     QRFPEDPEPVYYGLRTGLYLPQKEVFEEFRRRADAIKMPGHVLHLIDYLYAYLRGRMD
                     QANLSLDEFHRKYPSLNYYSEILRFVTTEIPPTEKSVKRVVFLSVDNFCKKMLRIGET
                     "
BASE COUNT          622 a          839 c          735 g          489 t
ORIGIN      
        1 actcccaata gtcaccggtg cgccgggctc ggtgaagtga cggggagaaa gattctccca
       61 cccccttttg caaacggaga acagaggaag ggctggtggc cccctgacgg cggagcggca
      121 gggggatcag aagttctcag gtggagtaac cagatcgagc ggagtgtctc gatttatcac
      181 catgggaacc gtgctcctgg acgggaatga gcatccacgg gcactgtatc tatggagaat
      241 caccgccgtg agggggatgg aacatcccca gcccatctcc tggcatgcca tcctgatact
      301 ccatttgcga gaacacccca aactcccgga acctttcgcc gaaaacgaaa ggggggtttc
      361 ggactggcca ccgtgagtgc tggcgccaat ttgaaccgat gcgcacggct accgccgcct
      421 tgctccagga tcaaaacccg aaccctcaat tcgcacggtt tcgggtaaaa tcgcataaaa
      481 aacccggata cgggcaaaag agcctcaggc gagattcgaa ctcgcgacct tgtccttacc
      541 aaggacacgc tctaccggct gagccactga ggcgcaacgc accacctatg ttggaggttt
      601 accctataaa aggttacggc aggagcggtg cagaaacagc cgatgagcgc gcttcaacgc
      661 ctcccggcat cgattgcctc cagcgccgcg atgctcgcga gcaccccctc cagcgtctcc
      721 acctcgacta tgggtatgac gccgctcctg tgcaccgcct ccatcacggc cgcaaaatcg
      781 atcgtccccg tcccgaccgg cgaatgcgtg tcccgcatac cgtcgttgtc gtgcaggtgg
      841 aaatgcgccg caggatgatc caggaaacag tccaggcagc cgttcaggtt cgcatggcca
      901 acgtcgagcg caagcccgat cccatcgatg agcggcagat cgtcacagga acgaaggaag
      961 aagtagtccg gaccccccat gttctccaca aagaacgtga ccgagaggtc atcagcacca
     1021 gcactcagtt cagagagcga cttctgaaac tgcctcgttg actcctcccg ctcctcgcgc
     1081 catgcatagt agcccgggtg aatcaccacg tccgccccca cctcagcagc gacagcgaag
     1141 gcctgcacca ggacatcgac gcttgcctgc cggatcggtt caaggaggct cgcaatgttc
     1201 acgccacgga atggcgcgtg aatgaagtag cgaaacgagt ggctctcgag cggttctgcg
     1261 gtctccagag aatgcagccc cccatccatc acctccacac accccgttat gggagcgagt
     1321 gtgtcaagcg ccgcatcgag cggctggtgc tgaaggcagt atgtcgagac gccaaacata
     1381 cagagtgttt cggcaggatc gataaatagg ttaggtacaa acccgcacgg ggagagcatt
     1441 cccgcgcaga gaactccagg tacgcatggt ttatgctatc cttgccgcat acacttacgt
     1501 atggctgacg gctttgtccc ggaggttgtc gtctcaaacc accttgagaa ggtaaaagcc
     1561 gagatcttct catacataaa ctccaacgcc agagccatag acgataactt cgccgctttc
     1621 ttcgggaaca tcgtcacgat cctcgacaat gccggcatcc ccatccctga ccaggtttac
     1681 gacgagttca tagactcgat cgcctacagg gtctttgacg ccagcaagcg ggccggagac
     1741 ctgaagttcc tcacccgcgc ctgcgagatc gcctgcggga tgaaacgaaa gaaggagcgc
     1801 aaggccggag tccaccttgc agccgcggtc aaactgatga agatcgggat gccgcttgca
     1861 gcaaccgcct ttctccagcc ctaccgcaaa ttcgatgccg ccgtcgggtg ctggtgcgcc
     1921 tactgctact acacactcta caaagagggg tcgtccgaac ccggctactc cgcgtccgaa
     1981 cgctggaact acctcaagac cgcacgccag cagatggagg aactcgggca ggcgcagccc
     2041 ggactgaacc gcctggtcaa aggagaactt gcagatgacc gctggctcat cgaaccgttc
     2101 tgggtgatga tcttcctcgc gaccgagtgg gtccccgaga accggtggct ccttgagatc
     2161 gggatcacac gcgcaaagca ggataagaac gaaccggcgc tggtgaaact gctccagctc
     2221 gggtttatcc ggttcccaaa cgacatgctc ttctaccgcg aggcctacca cctcaagttc
     2281 gagcagggtg acctcgcaga tgccctgggt ctcatccgcg accttctcca gaggtttccg
     2341 gaagaccccg aacctgtcta ctacggtctc cgtacgggtc tctacctccc ccagaaggag
     2401 gtctttgaag agttccggag acgtgccgat gcgataaaga tgcccgggca cgtcctccac
     2461 ctcatcgact acctgtatgc ctacctcagg gggaggatgg atcaggcgaa cctctccctg
     2521 gacgagttcc acaggaagta cccctccctt aactactact ccgagatcct gcggtttgtc
     2581 accaccgaga tccctccaac agagaagagc gtcaagagag tcgtattcct ctcggtcgac
     2641 aacttctgca agaagatgct caggatcgga gagacctgag ggtgc
//
LOCUS       DAOSZX010000141         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_30568_length_2609_cov_22.713782, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000141 DAOSZX010000000
VERSION     DAOSZX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30568_length_2609_cov_22.713782"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK196_08090"
     CDS             <1..405
                     /locus_tag="PK196_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HON41408.1"
                     /translation="TGEDTICERYNVGPGDIHGMVESVVWLVHASRRLAALFAPHLVQ
                     PIEELELRVRHGVRKELISLISLRGIGRVRARRLFNNGIDSIEALRAAGPEKIAKILG
                     KGITAQIFEQLEGLRDEIEEPTRTQSTLSWFG"
     gene            413..937
                     /gene="cgi121"
                     /locus_tag="PK196_08095"
     CDS             413..937
                     /gene="cgi121"
                     /locus_tag="PK196_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HON41409.1"
                     /translation="MTGMRYQIYRAVFTVEDKTRFLNDLRRIADDYMTHIILLDANHV
                     AGREHVAAALRHAWRSWESGEPIANSFEMEVLLYAAGTRQCHLAGSFGIHPGENQAYV
                     VICPPIPGAQERLADLVEFVDSDREEEVEIKPAKRKVLAEFFGITPEEVAVVGEDRFR
                     ELVLERVALLDVYR"
     gene            complement(928..1428)
                     /locus_tag="PK196_08100"
     CDS             complement(928..1428)
                     /locus_tag="PK196_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HON41410.1"
                     /translation="MDDSHHKPLTVIGAVITVSSTRNPETDTSGKSLLDLLASAGIEV
                     AYYTVIPDDQERIRAEVCKAVSCANCVIVTGGTGLTPSDVTIEAVVPLLEKIIDGFGE
                     LFRLKSYEEIGTAAILSRAVAGVVNGRAVFCIPGSTKAVTLAAREIILPEIQHILTHA
                     SAGTQR"
     gene            2012..>2609
                     /locus_tag="PK196_08105"
     CDS             2012..>2609
                     /locus_tag="PK196_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON41411.1"
                     /translation="MSDDKTGSFGLFKKYLANKRIFKNREVLRHNYRPQFLPHRKPQI
                     DELASILAPALTNETPSNILIYGKTGTGKTASVRYVGAELENASALAGTKCRVVHLNC
                     EVIDTQYRVLAQIANSLDDLDAHPSDSARNLIPMTGWPTDQVYTELKKQLESGGGVVV
                     IVLDEIDKLVKKSGDDTLYNLTRINSDLRLAKVSIIGIS"
BASE COUNT          621 a          711 c          702 g          575 t
ORIGIN      
        1 acgggggagg atacgatctg cgaacgctat aacgtcggcc ccggcgacat ccacgggatg
       61 gtggagagcg tggtctggct agtccacgca agccgacgcc ttgcagcgct ctttgccccg
      121 caccttgtgc agccgatcga ggagctggaa ctccgggtca ggcacggcgt cagaaaggaa
      181 ctcatctcgc tcatcagcct ccgcggcatc ggccgggtcc gtgcccgccg actcttcaac
      241 aacggtatcg actcgatcga ggcgctccgc gcggccgggc cggagaagat tgcaaagatc
      301 ctgggtaaag ggatcaccgc ccagatattc gagcaacttg aaggtctgcg tgacgagatt
      361 gaggagccca cccggacaca atcgaccctt tcctggtttg ggtgaccaat agatgacagg
      421 gatgagatac cagatctacc gcgcagtctt cacggtcgaa gacaaaacca gattcttaaa
      481 cgatctccgg aggatcgccg acgactacat gacacatatc atcctcctcg atgccaacca
      541 cgttgcaggc cgggaacatg tggcggcggc gctccgacac gcctggaggt catgggagag
      601 cggtgagcca atagcaaact cgtttgagat ggaggtgcta ctttacgccg cggggacacg
      661 gcagtgccat ctggccggat cctttggcat acatccgggg gagaaccagg cctacgtcgt
      721 catctgcccc ccaatcccag gcgcccagga acggcttgca gatcttgtag aattcgttga
      781 ctccgatcgg gaggaggagg tagagatcaa acccgcgaag cggaaggttc ttgcagagtt
      841 ttttgggatc acccccgagg aggttgcggt ggtaggcgag gatcggttcc gtgagctcgt
      901 gctcgagcgg gtggccctcc tcgatgtcta ccgctgagtg ccagccgagg catgggtgag
      961 gatatgctgg atctcgggga ggatgatctc acgtgcggcg agcgtcacgg ctttcgtcga
     1021 cccggggatg cagaagaccg cacggccgtt gacaaccccg gcaactgcac gcgagaggat
     1081 cgcggccgtc ccgatctcct cgtaactttt cagcctgaaa agttccccga aaccgtcgat
     1141 tatcttctca agcagcggca cgaccgcctc gatggtcaca tcgctgggtg tgagccctgt
     1201 tccgccggtg acgattacac agtttgcaca ggaaactgcc ttgcagacct ctgcgcggat
     1261 ccgttcctgg tcatccggta tcacggtgta ataggccacc tctatgcccg cactggcaag
     1321 gagatcaagg aggctttttc cactggtatc ggtctcggga ttccgggtcg aagagacggt
     1381 tataacagcg ccgattacgg tgagtggttt atggtgacta tcatccatgg ggactccccc
     1441 atatgatgta gcgcggattt tacttctttc tttctccaga agttaagaat aattgagagc
     1501 atacgacccc tgaccccttc gcttcgactg gaacgggggt aaccaaccag cagtcagaca
     1561 ggagaacagt gtgctccagg atctgtgatc atggtgaagg aggatctcaa ctctgtgatt
     1621 agcaggacgc gaacatagtg gggatctacc ttttaccatg tgagcgtata aaaaaacttc
     1681 tattatccgc acagttttat tctatgtcct cctttttacc tcttttcaaa tggatatttt
     1741 tatatagttt cagtttcata gatgaaaatg actatcgtca tcatgtttta tgaacgatac
     1801 aataggttgt atcgtcatca cgctccactg gaaacaaagg ggtgtgcccc cccgatcttt
     1861 tcgcctgctt ctttcaagtt tgcctgcagc cagattttat tagaccctcc atggttccac
     1921 tggaaacgaa ggggttatct atccacagaa cgatcgtatt aagagttctc aacacgaaaa
     1981 ctccctccct tgcaaacctc ccggtgacag aatgtcggac gataaaacag gttcttttgg
     2041 cttatttaag aaatacctgg ccaataaacg tatatttaag aatagggagg tcctgcgtca
     2101 taactaccgc ccacagtttc tacctcaccg gaaaccccag atcgatgaac ttgcgtctat
     2161 cctcgcacca gctctcacaa atgagacccc ttcaaatatc ctcatctacg ggaagactgg
     2221 aacagggaag acagcttctg tccggtatgt gggagcagaa ctcgagaatg ccagcgcgct
     2281 tgccgggacg aagtgcaggg tcgttcacct caattgcgag gtgatcgaca cccagtaccg
     2341 ggtccttgca cagattgcaa atagccttga tgaccttgat gcacacccga gcgacagcgc
     2401 cagaaacctc atccccatga cggggtggcc gaccgaccag gtctacaccg aactcaaaaa
     2461 gcaacttgag agcggcggcg gggttgtggt gatagtcctt gacgagatcg ataaactcgt
     2521 caaaaagagt ggtgatgaca ccctgtacaa tcttacccgg atcaactctg acctccgact
     2581 tgcaaaagtc agcatcatcg ggatctcaa
//
LOCUS       DAOSZX010000142         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_30607_length_2607_cov_17.800157, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000142 DAOSZX010000000
VERSION     DAOSZX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30607_length_2607_cov_17.800157"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..192
                     /locus_tag="PK196_08110"
     CDS             37..192
                     /locus_tag="PK196_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41412.1"
                     /translation="MNRIAIRFSSCIPRTLHPAAWDAVKPALKVLTNALQPVFLVVCR
                     LQESGVR"
     gene            complement(215..1156)
                     /locus_tag="PK196_08115"
     CDS             complement(215..1156)
                     /locus_tag="PK196_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HON41413.1"
                     /translation="MFTIEEIRTKRGILQYRRERLTGLQCRISPARMEREIDTAPIMP
                     PSRDDCPFCPGALETSTPTFEDGSRIRRGESVTFPNLYPFAGRHVVTVITPEHAPDRF
                     DRKNLTDAISGTFEALIGFPGYASINWNYLPSAGASIFHPHLQGIADEKPTHLAEIYL
                     TAGRRYLAEHGRPYWDDFAEHERTSERFLFEDEIFWSANPVPLGEREVRGILPISTLE
                     EFGPYIEPLADGILRVIDFYRGLGTYAFNAAIHFDRDGNSGSGHRAFCSIIARLNPNT
                     LSMCDSAFMERLHLEPVILTLPEDVGALFRIKRSTSH"
     gene            complement(1284..2276)
                     /locus_tag="PK196_08120"
     CDS             complement(1284..2276)
                     /locus_tag="PK196_08120"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HON41414.1"
                     /translation="MTTYGIEFVPGAINVKQVVKYTKLAESKDIDYAWITNHYNNRHA
                     YPTLAMIAANTDTIKLGPGIMNTFTDTPAAIASFAATLYEISDGRAVLGIGPGDLSTL
                     PKIAIQPVKPIARLREGVEQIRKLLTGEEVKKTGKMEFFDYDGAKLTGVTLPGKKGIP
                     IYIGAQGPKMLELAGEIGDGALINASNPKDFEVAIPIIKKAMEKVGKKSFDVGAYTAM
                     SIDMDEKKARNAAKIVAAFIAAGSPPDLLKRHNLDLNNVAKIKEALGRFDFKTVGGLV
                     GDAEIDAFTIAGTPDMVKQKCEDLAKAGVTQIIFGSPLGPDMTTSIRLLGKYIV"
BASE COUNT          490 a          679 c          835 g          603 t
ORIGIN      
        1 catggataat taggtctcgg gtgcggttca tggcagatga accggatcgc catacgcttc
       61 tcaagttgca taccgcgaac ccttcacccc gccgcatggg atgccgtcaa gccggccctg
      121 aaggttctta caaacgcctt gcagcccgta ttcctggtcg tttgccgcct ccaggagagc
      181 ggcgtccggt gagaccgatc ccgagagagc gggatcaatg gctggtggat ctctttatcc
      241 tgaagagtgc gccaacgtcc tcagggagcg tcaggatgac gggttcgagg tgcagcctct
      301 ccatgaacgc cgaatcgcac atggagaggg tgttcgggtt taaacgggcg attatggagc
      361 agaacgcccg atgcccactc ccggagtttc cgtctcgatc aaagtggatt gcggcattga
      421 aagcgtatgt gccaagaccc cggtagaagt cgatgacccg gaggatgccg tctgccagcg
      481 gttcgatgta tggcccgaac tcctcaagcg tcgatatagg gaggatcccc cggacctccc
      541 tctcgccgag ggggacgggg tttgcagacc agaagatctc gtcctcgaag agaaaccgtt
      601 ccgaggtgcg ttcgtgctcc gcgaaatcgt cccagtaggg gcgtccgtgt tcggcaaggt
      661 agcggcggcc ggcggtgagg tagatctcag ccagatgggt gggtttctca tcggcgatcc
      721 cttggaggtg gggatgaaag atgcttgcac cggctgaagg gaggtagttc cagttgatgc
      781 ttgcatatcc aggaaatccg atcagcgctt caaatgtgcc ggatatggca tcggtgaggt
      841 ttttcctatc aaaccgatcg ggtgcatgct caggtgtgat caccgtgacg acgtggcggc
      901 cggcaaaagg gtagaggttc gggaacgtga cgctctcccc gcgccggatt cggctgccgt
      961 cttcaaacgt gggtgtcgag gtctccaggg cgccggggca gaaggggcag tcatcacgtg
     1021 agggtggcat tataggggcg gtgtcgatct ctcgctccat acgggcgggg ctgatcctgc
     1081 actggagccc ggtgagtctc tccctgcggt actgcaggat cccccgtttc gtccggatct
     1141 cttctatggt gaacatggcc ctccgtaccc cgtttggttt gccgggtgta taaacctgtc
     1201 agaacagcgg atgaggagga tagagacagc ggcgtaaact tcagcggtgg tattataaaa
     1261 agggaagggt gttttttccc gcctcagaca atgtacttgc ccaggaggcg gatggaggtg
     1321 gtcatgtcgg ggccgagcgg cgagccgaag atgatctggg taactccagc cttcgcgaga
     1381 tcctcgcact tctgcttgac catgtcgggg gttccggcga tggtgaaggc gtcgatctcg
     1441 gcgtcgccga cgagtccgcc gacggtcttg aagtcgaagc ggccgagcgc ctccttgatc
     1501 tttgcgacgt tgttcaggtc aaggttgtgg cgcttgagga gatcgggcgg tgaaccggcg
     1561 gcgatgaacg cggcaacgat cttcgcggcg ttccgggctt tcttctcgtc catgtcgatg
     1621 gacatggccg tgtaggcgcc gacgtcgaaa ctcttcttgc cgaccttctc catcgccttc
     1681 ttgatgatcg ggatggcgac ctcgaagtcc ttcgggttgg aggcgttgat cagggcaccg
     1741 tcaccgatct caccggcgag ttcgagcatc ttgggtccct gggctccgat atagatcggg
     1801 atccccttct tgccgggcag ggttacacct gtcagcttgg cgccgtcgta gtcgaagaac
     1861 tccatcttgc cggtcttctt gacctcttcg ccggtcagga gtttccggat ctgctcgaca
     1921 ccttcgcgca ggcgggcgat gggcttaacg ggctggatcg cgatcttggg aagcgtcgag
     1981 aggtcgccgg gcccgatacc aaggacggcg cgtccgtccg agatctcgta taaggtggcc
     2041 gcgaatgacg ctatcgctgc cggggtgtcg gtgaaggtgt tcatgatacc cggtcctagc
     2101 ttgatcgtat cggtgtttgc cgcgatcatg gcaagggtcg ggtatgcgtg gcggttgttg
     2161 tagtggttgg tgatccaggc gtagtcgatg tccttcgact ctgcaagctt ggtgtacttc
     2221 accacctgct ttacgttgat tgctccgggc acaaattcaa ttccatatgt agtcaaggct
     2281 attcacctca ttgagtagtt acgtgcaggg agttatataa attatcattt tgtgctcggc
     2341 cggactgcac ccaatcgcgc aggcagctcg aaaagtggat gggtggaggg ttttacaggg
     2401 tatcggctgg tctaacaacc ccgtcttgcg ggcaatccgg aatggttaag ggtgcccatc
     2461 ggcagcgtta aatgccgcag ggaaatatat atcttcgtcc ggccatataa tcatcaatag
     2521 ttctcgataa gttccatgct tctttcgcgg cggaatctct tcggctgtac atggaaggtg
     2581 ccaggtaagg ggtataatcg gtatgcg
//
LOCUS       DAOSZX010000143         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_30742_length_2599_cov_16.486635, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000143 DAOSZX010000000
VERSION     DAOSZX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30742_length_2599_cov_16.486635"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK196_08125"
     CDS             <1..639
                     /locus_tag="PK196_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41415.1"
                     /translation="MHFFILPEAPDVTRIENITHLESPAYHPAILDLWEIAVESTGVE
                     NESARLLQLEVDLAEDGAIRVIWLFFYGDRDGEQHGYEAYVGPGGTVSAKSQKFNFSV
                     QGVHPLELLREVDAIALEDIPFRDRGMRLLVSVNAYGDHYNETRGRLYKVSKGAFMPV
                     KEATFGPDASWYTITIIPNSGAGSPPSGEELPDCVIAFTRQDLAAADSVIYG"
     gene            complement(733..1479)
                     /locus_tag="PK196_08130"
     CDS             complement(733..1479)
                     /locus_tag="PK196_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HON41416.1"
                     /translation="MVNQPALARFALFLIVFTLLITPGSATSTILSEGEFPEMVPDDP
                     TLEYVWNIPLKLILLDFVFMTAPLLFLPVQLLISVSVWLCLGQKRISRKNALEHDTRR
                     AAYLCIRENPGINHATLSRRLGVSVGTLRYHIEILCETGQIVSENDHGFLRYYTNSRA
                     AREGKRGAYPLNETRKRMLDLLGRNPGMMRKEVASALGITGASVTWHMGLLIQSGAVR
                     SEKDGRMVRYFPRHDVVRHSRADRSVDATG"
     gene            complement(1520..1711)
                     /locus_tag="PK196_08135"
     CDS             complement(1520..1711)
                     /locus_tag="PK196_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41417.1"
                     /translation="MTLKIISPSGSTYAYYHDTDLGGGKDAQIRTRISSPEAGMWRFQ
                     VYGEEVQDTQSYFFEAATE"
     gene            complement(1708..1956)
                     /locus_tag="PK196_08140"
     CDS             complement(1708..1956)
                     /locus_tag="PK196_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41418.1"
                     /translation="MMTYNHFFALMLALVLMVPSAFAGSSECSVNTAPGEEAPTGDAG
                     ICAFYSIQQGETNHHSANVPPGSSTLFVNLDRFIPSSR"
BASE COUNT          564 a          745 c          730 g          560 t
ORIGIN      
        1 atgcacttct tcatcttacc ggaggccccg gacgtcaccc gtatcgagaa tatcacccat
       61 cttgagtccc cggcatacca tccggccatc ctggacctct gggagatcgc cgtcgagagc
      121 accggcgtcg agaacgagtc ggcgagactg ctgcaacttg aggtcgatct cgccgaagac
      181 ggagcgatcc gggtaatatg gctcttcttc tacggcgata gggacggcga acagcacggc
      241 tacgaggcgt atgtggggcc gggcggaacg gtatcggcta aatcgcagaa gttcaatttt
      301 tccgtgcagg gggtgcaccc cctcgaactc ttgcgggagg tcgacgctat cgccctggag
      361 gatattccct tccgggaccg gggtatgagg ctgcttgtga gcgtgaacgc gtacggggac
      421 cactacaacg agacccgggg aaggctctac aaggtctcga agggggcgtt catgccggtg
      481 aaagaggcga cgtttggtcc ggacgcatcc tggtacacca tcacgatcat cccgaactcg
      541 ggtgcggggt ccccgccgtc cggagaagaa cttccggact gtgttattgc gttcacccgg
      601 caagacctcg cggcggccga ctctgtgatc tatggatagg tgggcgtgcg aaaccgacag
      661 agccaggact cgttcaccgt ctccagcccg ggcgcccccg gattgttcag cccacccctg
      721 ggtgacgaag gtctagccgg ttgcgtccac gctcctgtcc gccctggaat gccgcacaac
      781 atcatgcctg gggaagtatc gcaccatcct tccgtccttc tcgctccgga cggcaccact
      841 ctggatgagc agccccatat gccaggtcac cgatgcaccg gtgataccga gcgcggatgc
      901 gacttctttt cgcatcatcc ccggattccg gccgagaaga tcgagcattc tttttctcgt
      961 ctcgttgagg ggataggcgc ctctcttgcc ttcgcgggcc gccctgctgt ttgtatagta
     1021 ccgcaaaaac ccgtggtcgt tctccgatac tatctgcccc gtctcgcaga ggatctcaat
     1081 gtggtaccgg agcgttccaa cgctaacccc cagcctgcgg gagagcgtag catggttgat
     1141 tccggggttc tcccggatgc agaggtacgc cgcacggcga gtgtcgtgtt cgagggcatt
     1201 cttccgggag atcctctttt ggccgagaca gagccatacg gacaccgata tgaggagttg
     1261 caccgggagg aagagcaggg gtgcagtcat gaagacgaag tcgagcagga tcagtttcaa
     1321 cggtatattc cagacatact ccagggtcgg atcgtccggt accatctctg gaaactcacc
     1381 ctcgctcagt atggtgctgg tggcgctccc cggggtgatg agcaacgtga agacgatgag
     1441 gaacagtgcg aacctcgcga gggcgggttg gttcaccatg aagagaatta gacggccgta
     1501 aatataaaaa agatttgatt tattccgtcg cagcttcaaa gaaataactc tgcgtatcct
     1561 ggacttcttc cccataaacc tggaaccgcc acatcccggc ctccggagag gagatccgtg
     1621 tgcggatctg tgcgtcttta ccaccaccca gatcggtgtc gtgatagtag gcgtaggtgc
     1681 ttccgctcgg cgagatgatc ttcagcgtca acgactgctg ggaatgaacc ggtccaggtt
     1741 cacaaacagc gttgaactcc cgggaggcac attcgcgctg tgatggttcg tctccccctg
     1801 ctgtatagaa tagaaggcac aaataccggc gtcgcctgtc ggggcctctt ctcccggggc
     1861 ggtgttcacg ctacattcgc tgctcccggc aaaagccgac ggcaccatca ggaccagtgc
     1921 aagcatcagt gcaaagaagt ggttatatgt catcatacta cgggagaact tctcaaatgc
     1981 cttcacttaa gttttctgtc gatcctctct acacgttccc gggtccggcg cagaccggtt
     2041 tcccggtagt cggacccgcg cgatatttga gaggagtgtg cggggcgggt accggtgccg
     2101 cccctccctc cactgcatgg gctccacgtc gctcgataca gcacaaatcc ggcacgcttc
     2161 tgggaccaaa agagatcaag ccgtcccaac gtgtagagag gttcgacaga aatcgtttat
     2221 agaggaacgt tgaactctca atcgtgggat accggaaccg gtatcccgaa gcgcgggggt
     2281 ccctccatcc acggttgacg cccggatgat agagccccgc atgcccggag gcaaagggaa
     2341 tgtactcatt ccgtgtactt aaaatatgtg cctcttctgt cctccgggaa ggaatcgagg
     2401 agaacaatca tgaatgaact acgcaggatt ggaagaatat ccatcctctt gggagtgctg
     2461 ctttgtgtag cacttatatc gaccccaaac accgcatgat ttagggatta tactctccaa
     2521 atcatcctaa tttttccaat cccacctctc aaaccgcaag atctatatac ttataatccc
     2581 taatattata tcatatgga
//
LOCUS       DAOSZX010000144         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_31111_length_2572_cov_19.608264, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000144 DAOSZX010000000
VERSION     DAOSZX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31111_length_2572_cov_19.608264"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1366
                     /locus_tag="PK196_08145"
     CDS             176..1366
                     /locus_tag="PK196_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HON41419.1"
                     /translation="MAAREIVPGVFAVGAIDWDRRLFDELIPLPDGTTYNSYLVRGSE
                     KTALIDTVDPTKTSELIANLESLGVDKIDYLIASHAEQDHSGSIPAVLERFADAKVVT
                     NQKCRDLLIALLHLPEERFIVIDDGDTLDLGGKTLEFIIAPWVHWPETMLTYLAEDRV
                     LFPCDLFGSHYATSSLYVPDECAVYEAAKRYYAEIMMPFRASIRGHLEKLATREIEII
                     APSHGPVHRRPAFIIDAYRDWTGDAVKNIVVLPYVSMHGSTQAMVDHLLDALINRGIP
                     AQPFNLTKTDTGDLAKALVDAATIVIGAPTVIFGPHPQVVYAAYLANLLRPKTRYATV
                     IGSYGWGGKTVDTIKGMLDRLKVEVLEPVYIRGYPTAEDFAALDRLADEIERKHREAG
                     IIST"
     gene            1450..2478
                     /locus_tag="PK196_08150"
     CDS             1450..2478
                     /locus_tag="PK196_08150"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HON41420.1"
                     /translation="MMPPSDFKRVIAESPYVFIIGVAGDSGSGKTTFTHAIREIFGED
                     LVSTITTDDYHKYDRQQRRELGITPLAPEANQLDLLEEHLAELKAGRAILKPVYNHNT
                     GRFDPPVPFSPTKILIVEGLHPFLTPRLRALIDFKLYVDPDPGVKRTWKIRRDVEQRG
                     YALEEVEREMEERKPDFERYVAPQCQYADAVIRVAFSKYGREASEKHNIYCVTITQSK
                     LERSIGDVDLSIDLFPLLSLSERNFMVEFTLEEILGRKMGALTFDGELNDAVVRKLER
                     NIEIQTQVEPIDLVEAGGYLTAGEVAQLILAWRIINRRIFIESGLKAVETEDGAASGN
                     GACRSGRD"
BASE COUNT          604 a          722 c          746 g          500 t
ORIGIN      
        1 gttagagcga aactgttatc agcattcgcg ccggagaaag gtttgccgac cggtgcggca
       61 cgaagtgtac tcgccggcac ggtcaatcaa gaaccaatgg agctgaaaaa ggcatgtcga
      121 atacgctgat acaccggata cccggtgggt tggaggatac ggaggtgatg ggataatggc
      181 cgcacgtgag attgtgccgg gcgtctttgc cgtcggcgca atcgactggg accgccgact
      241 cttcgacgaa cttatcccgc tccctgatgg caccacctac aacagttacc tggtgcgggg
      301 cagcgagaag acggcgctga tcgataccgt cgacccgaca aagaccagtg aactcatcgc
      361 gaacctggag agtcttggtg tcgataagat agactatctc atcgccagcc acgccgaaca
      421 ggaccactca ggatctatac cggcagtgct tgagaggttc gctgacgcga aggttgtgac
      481 aaaccagaag tgccgcgacc tcctgatcgc tcttctgcat ctcccggagg agagattcat
      541 cgtaatcgac gacggcgaca ccctcgacct gggtggtaag acactggagt tcatcattgc
      601 cccatgggtc cactggccgg agacgatgct gacctatctc gcggaggatc gcgtcctatt
      661 cccctgtgac ctcttcggtt cgcactacgc gacgagttcg ctctacgtcc ctgacgagtg
      721 cgcggtctac gaggctgcaa aacgctatta cgccgagatc atgatgccgt tccgcgcgag
      781 catcaggggg caccttgaga aactcgcgac gcgcgagata gagatcattg caccgagcca
      841 cggccccgtt cacagaagac cggcgttcat aatcgatgct tacagggact ggacgggcga
      901 tgccgtgaag aatatcgtgg ttctccccta cgtctccatg cacgggagca cgcaggccat
      961 ggtcgaccat cttcttgacg ccctgataaa ccggggtatc ccggctcagc cgttcaacct
     1021 cacaaagacc gatacaggcg atctcgcaaa agcactcgta gacgccgcaa cgatcgtgat
     1081 aggggcgccg acggtcatct tcggtccaca cccgcaggtg gtgtatgcgg cataccttgc
     1141 aaacctgctc cgcccgaaga cgcgctacgc cacggtgatc ggctcctatg gctggggtgg
     1201 aaagacggtc gataccataa aagggatgct tgaccgactc aaggttgagg ttctggaacc
     1261 ggtctatatc aggggttacc cgaccgcgga ggattttgcc gcgctcgacc ggctcgccga
     1321 tgagatcgag aggaaacaca gggaagcagg gataatctcc acctgaaccc cgccattttt
     1381 ctgctggacg tcctctctca ccgaaatatt aaccgtcccg gataccgatc agtttatctg
     1441 agttgtgtta tgatgccacc atccgatttc aagagagtca tcgcggagtc gccatacgtc
     1501 ttcatcatcg gtgttgctgg agacagtgga agcggaaaga caacgttcac ccacgccatc
     1561 cgggagatct ttggcgaaga cctggtctcc acaataacaa ctgacgatta ccacaaatac
     1621 gaccgccagc agcgcagaga actcgggatc acgccgcttg cgccggaagc aaaccagctt
     1681 gacctgctgg aggagcacct tgccgaactg aaggctggac gcgcgatcct gaaaccggtc
     1741 tacaaccata acaccggcag gttcgatcct cctgtgcctt tcagcccgac aaagatcctt
     1801 atagtggagg ggctgcaccc cttcctcacc ccgcgactcc gggcgcttat cgacttcaaa
     1861 ctctacgtcg accctgaccc cggcgtcaag cgcacctgga agatcaggcg cgacgttgag
     1921 cagcggggct atgcccttga agaggtcgag agagagatgg aggagcgtaa accagacttt
     1981 gagcgctacg ttgcgccgca gtgccagtat gcggacgctg ttataagggt cgccttctcg
     2041 aagtacggcc gtgaggcaag cgagaagcat aacatctact gtgtcaccat cacccagagc
     2101 aagttagaga gaagtatcgg cgatgtcgac ctctccatag acctcttccc tctcctctca
     2161 ctctcagaga ggaacttcat ggttgagttc acgctcgagg agattttggg gagaaagatg
     2221 ggggcgctca cgttcgatgg agaattaaac gacgctgtgg tgcggaaact ggagaggaac
     2281 attgagatcc agacacaggt ggagcccata gacctggtcg aggcgggggg ttacctgacg
     2341 gccggggagg tggcccagct catccttgcc tggcggatca tcaatcggcg catcttcatc
     2401 gaaagtggcc ttaaagcggt cgaaactgag gatggggccg caagcggaaa cggtgcctgc
     2461 aggtccggcc gggactgaat ccggaaattt tttccggcgc atgatggcaa aatggccgtc
     2521 ctggagaaga tagatatgag cgcccggaga atctgtatcg atggatagga tc
//
LOCUS       DAOSZX010000145         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_31712_length_2534_cov_12.845099, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000145 DAOSZX010000000
VERSION     DAOSZX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31712_length_2534_cov_12.845099"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(648..938)
                     /locus_tag="PK196_08155"
     CDS             complement(648..938)
                     /locus_tag="PK196_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HON41421.1"
                     /translation="MVSDRILREISRDDYFSSAVRYFWGKRVSQAQEQNSRGVKDQGF
                     RSEATGGKHMDGFFLLIRDLLVDIGVPEDDICTQKRSPLIFRDTSVQRSSGI"
     gene            complement(952..1866)
                     /locus_tag="PK196_08160"
     CDS             complement(952..1866)
                     /locus_tag="PK196_08160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON41422.1"
                     /translation="MPVEIREVAESEGQRAIPMIAKNLSLMKEIEAISGNLPTQHRFV
                     NADSRNLAFLPDESVHLVVTSPPYWTLKKYPEKDEQLGQIEDYDLFLEELDKVWRHIH
                     RILVPGGRLVVVVGDVCLPRRGAGRHVVMPLHASIQEHCRKIGFDNLAPIIWYKIANA
                     CFEADSPGSFLGKPYEPNGIVKNDIEYILFQRKPGGYRKPTLETRLLSVISEENQKTW
                     FRQIWDLKGASTREHPAPYPLELAERLVRMFSFVGDTVLDPFGGTGTTALAAARWGRN
                     SISVEIEEKYFAIEIERFTHQAKQTRLF"
BASE COUNT          534 a          739 c          653 g          608 t
ORIGIN      
        1 cacgatgccg ctgatcggcg ctgcggtgcg ctcggtgctg gcgatggggt aaataccccc
       61 ttttctcatc ggggcggcat ctcaaggttt tcgcaggcct ttcccatcat cagattccag
      121 cagcggcatc attgtggtta ctccgccgcc tgatggagga tggagttatg ctatttcgta
      181 aaaattgttc agatgggaac acaaagaccg caggaaccgg taccctgata agatctcgtc
      241 cggctcggca tagttccgcg gccgcatctt atcctcttcc ttcgcaagca ggtagcagac
      301 ctcggaatag actctttccc gtaccaactt gaggcaggtc tggtgatagc gttcgatata
      361 ggaagcgtcc ttaaactccg gaaatacctc aaaatgtggt tcgttattcc tgacaccctt
      421 ccgtgaccgt tcgcagtctt ccagcaggag catatacccc agccaaggag agggggatgt
      481 cttgaacgcc ccttcccggt aggcggtcca gagatcatac gcgctcccca gaacctcctc
      541 cgtccggttg ttgatattgt tcccgaacga tggtcccttc tgggacttca gttcaaggac
      601 ggcgaccagt tccttcttgc cgtcaggaat gaggtgcttg atgatgatta gatcccactg
      661 cttctctgca cggaagtatc ccggaagatc aagggagatc tcttttgtgt gcagatgtca
      721 tcttcaggca ccccgatatc cactagcaga tcacggatga gcaggaagaa tccatccata
      781 tgttttccgc ctgtcgcctc cgaacgaaag ccctgatctt tcacaccccg gctgttctgc
      841 tcctgtgcct gcgatactct cttcccccag aaatatcgta ccgcgctgga aaaatagtcg
      901 tcccgggata tttcccggag tattctatcg ctcaccatgg atctacctca atcagaacaa
      961 tcgggtctgt tttgcctgat gagtaaaccg ttcaatctct atggcgaagt atttttcctc
     1021 tatctcgacg ctgatgctgt tccggcccca ccgtgctgca gcgagggcag ttgtccctgt
     1081 accaccgaag ggatccagga cggtatcccc cacaaaagag aacatccgca caagcctctc
     1141 ggccagttcc agagggtagg gtgcaggatg ctctctggtt gatgctccct taagatccca
     1201 gatctggcgg aaccatgtct tctggttctc ctccgatatg accgagagca atcgtgtttc
     1261 aagtgtgggc ttccggtaac cgccaggttt cctctggaag aggatgtact ctatatcgtt
     1321 cttgacgatc ccgttgggtt cgtaaggttt gcccaagaac gatcccggtg aatctgcctc
     1381 aaaacaggcg ttggctatct tataccagat gatcggcgcc aggttgtcaa atccaatctt
     1441 tcggcagtgt tcctgaatgc tggcgtggag aggcatgacg acatgacgcc cggcccctcg
     1501 ccggggtaag cagacatcac cgacaacgac gaccaggcgg cccccgggaa cgaggatccg
     1561 atggatgtgt ctccaaacct tgtcgagttc ctccaggaag aggtcatagt cctcaatctg
     1621 tccgagttgt tcgtccttct cgggatattt cttcagcgtc cagtagggag gcgatgtcac
     1681 gaccagatga acgctctcat cggggagaaa agcaaggtta cggctatctg cgtttacgaa
     1741 acggtgttgt gtcggcagat tgccggatat ggcttcaatc tctttcatca gcgagaggtt
     1801 tttggctatc atcggaatgg cacgctgtcc ctcgctctcc gccacctcac ggatctcaac
     1861 cggcacatac tctcccaggg gttgatcctg cccggtttta ttgatgagaa acgatctctc
     1921 ggcaaagatg ggagccgtac ccggtatctg aaacggtctg gaactcaagc ttctggttct
     1981 atgattgata tcccttctta cataactgca ccggagtatg ccctctcaac ggttgtccac
     2041 cccgattcgt tctgcgagcc gaccctcaga tgcggagacc ccgtgtattg ctgctcctca
     2101 tcagagaacc caggcccgac cgggcggcgg agcaggccca caggttcgac ggcgtgcccc
     2161 atgcctatcc tgtaatatca cccgcttata gcatcaaggt ggcgccgtgc ggggtgaaag
     2221 taaatggacc agggggtgca attcccccca agagctaaag ccctgggcat tcttgctccc
     2281 cttgaacccc gttatttgtc agaaaggccg gaagaacaca acgggtaacc ggcaccgcca
     2341 gtgaggatgg acagccagga ttcggcaaaa aggtggtggg cacccccggc gaagtcttac
     2401 ccatcctcct tcccgagcat gtcccgtgac ttcttctgca tctccgcatc gatatcggtc
     2461 tcgatgctct ttaccgcctc ctccgcggtc tgaaccacgt cccggcccgg gatctccccg
     2521 aggatatcct ccgg
//
LOCUS       DAOSZX010000146         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_34128_length_2388_cov_16.734676, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000146 DAOSZX010000000
VERSION     DAOSZX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34128_length_2388_cov_16.734676"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK196_08165"
     CDS             complement(<1..382)
                     /locus_tag="PK196_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41423.1"
                     /translation="MESKFNRRLIIMGVLAFIGAALLVATVALTCTTLVALITGVDGL
                     PQDLDVREVWGEHLENASIIHLTEQDLEQFPALNSVIRGDNRDPHPWKLEGSPNDDPD
                     ERKIGRAPVTGTEHLVLIESFGIDA"
     gene            complement(554..1042)
                     /locus_tag="PK196_08170"
     CDS             complement(554..1042)
                     /locus_tag="PK196_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41424.1"
                     /translation="MNRKIVCVIVLMVGLAGALAYVMSYAREDVVVNTTTLGGRIVFL
                     DNISHGTYYLGDASADPERNMSFSNPVPFVEAGIGGSTFTATWRDIRNDEPNDEKIAV
                     FTLTVWDPAGRPYSTSLESGQMGSGLMSVSLSSPESGEGQFSLTSTILERKLNISAPA
                     GL"
     gene            complement(1097..1438)
                     /locus_tag="PK196_08175"
     CDS             complement(1097..1438)
                     /locus_tag="PK196_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41425.1"
                     /translation="MSTDGDGNTASVAGVGCVTAPAGQNTFTATYSNDNDRDNDGQGA
                     IYRLTVWDALGVAHTTEKRVDWATSGTISVTFNSQGSGDAQYELWCETHDWFDTTMAV
                     GGPYIGDLDFA"
     gene            2171..>2388
                     /locus_tag="PK196_08180"
     CDS             2171..>2388
                     /locus_tag="PK196_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HON41426.1"
                     /translation="MGSAKRKYGAFVLLLVVAFLAVLPRVGKGGCRVHDSSFTSDDPM
                     TTLGLSDTVDEGETNRHQFYVGSEVAYL"
BASE COUNT          538 a          705 c          600 g          545 t
ORIGIN      
        1 ttgcatcgat accgaacgac tcgatgagta caagatgttc tgtacccgtt accggagctc
       61 tcccaatctt gcgttcgtcg ggatcgtcat tggggctgcc ctccagtttc catggatggg
      121 gatctctgtt atctccccgg atcaccgaat tgagcgccgg aaactgttct aaatcctgtt
      181 cggtcaggtg aatgatggag gcgttttcca gatgctctcc ccagacttcc cgcacatcca
      241 gatcctgcgg aaggccatcg actcccgtga tcagggcaac gagggtggta catgtgagcg
      301 cgaccgtcgc gacaaggagg gcagcgccga tgaacgcaag gactcccatt ataatcagac
      361 gtctgttgaa tttcgattcc atataaccgc cctcatctcc ttcatcggat tctgccggga
      421 ggggatcgtc ctatttccat accagtggtt cccattaatt tttctaacac acgttttgct
      481 tcagatttcc gtatccaaac ctacagagcc aggataatat cttttcaacg ggaagggcgg
      541 cgcgttcacg ccctcacaag cccgctggag cagagatatt caactttctt tcgagtatag
      601 tcgatgtgag cgaaaactgc ccttctccag attcgggtga ggaaagcgat acgctcatca
      661 atcccgaacc catctgcccg gattctagag aggtgctata tggtctccca gcaggatccc
      721 agaccgtcag ggtgaagacc gctattttct cgtcgttggg ctcatcgttc cggatgtccc
      781 tccacgtagc tgtgaaggtg ctccctccga tcccggcctc aacgaaagga accgggttag
      841 aaaacgacat gtttctctcg gggtcggcag aagcatcgcc gagataatag gtaccatggg
      901 agatattgtc cagaaagaca attctgccgc ccaaagtggt agtattcacc acaacatcct
      961 ccctggcata cgacatcaca tacgccagag caccggcaag acctaccatc aagacgatga
     1021 cacacactat tttcctgttc ataatgacca ccactgcggt tcaaaaatag tgcatccatg
     1081 aagatgcacc cccttttcag gcaaagtcaa ggtcgccgat gtagggaccc ccaactgcca
     1141 tcgtagtatc gaaccagtcg tgggtctcac accacagttc atactgagcg tcgccgctgc
     1201 cctgtgaatt gaaggtcacg ctgattgtac cactggtcgc ccagtcaacc cttttttcgg
     1261 tagtgtgcgc cacgcccagg gcgtcccata ccgtcagacg gtagattgcc ccctggccgt
     1321 cattgtcccg gtcgttatca ttgctgtacg tcgccgtgaa ggtgttctgc cctgccggag
     1381 cagtgacgca ccccacaccg gccacgcttg ccgtattgcc gtcgccatcg gtagacatgc
     1441 tccccagatg tatggtggcc agaatcccga tgtcgcccgt attctcctct actgacattt
     1501 cagaatcggc ttgagccgca gaaactgccc cggcggatac cgatacaaag agcatcagta
     1561 ctgccagcat tgacagaagc ccgtttcctt tcttcatcat caggtacctc cgttttcggt
     1621 tcccggaggc acacgcacac gtttaagtct gcagagtgtg tacactctca ttgcctccag
     1681 gcacgcggga cgttcacccg ccgtttggca tatcagccgg gtggacctcc gcactggtgc
     1741 aagcaccgcc tgcggcgccc acaagtgttc acttgtcagt tttgagataa accgtttatg
     1801 ggacaaacac caattcgaca cgcgactttt ctggaaaata gcagggataa cgccccggga
     1861 tcgccataga caactccacc tgcatgagac cctcacagga gaattccctc ccgatggaaa
     1921 atgcagggct ggtatgagca gcattgttcc gggagattcg gtactggggt tccctccccg
     1981 tgcgttgcgc gcaggccgtc ccgctacgcc cgcaccgcac caatctcttc agatatagtg
     2041 gctccaggca cggtacggcc gtgttgctcc tcaaacacgt tcgcctccag gaaaggaaga
     2101 gggcagagac gagaatagag ttggtgtttg tcccataaaa ctcataaaac gcggataacg
     2161 atcctccttc atgggatcag caaagagaaa atacggggct tttgtcctgc tgctcgtcgt
     2221 cgccttcctt gccgtgctcc cccgtgtcgg caaaggagga tgtcgggtac acgattcgtc
     2281 cttcacgtcg gacgatccga tgacgacgct agggctctcc gacaccgtcg acgagggcga
     2341 gaccaacagg caccaatttt atgtcgggag tgaggtcgca taccttga
//
LOCUS       DAOSZX010000147         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_34805_length_2351_cov_16.755662, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000147 DAOSZX010000000
VERSION     DAOSZX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34805_length_2351_cov_16.755662"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..545
                     /locus_tag="PK196_08185"
     CDS             300..545
                     /locus_tag="PK196_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41427.1"
                     /translation="MTAEKTTRELVYETNRDVKWICRTLQQMEARDEDFEARVRALEG
                     WQAEREGAERRLSTVSAGAGGIVGGVVAVILRVIGGG"
     repeat_region   1556..2035
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1633..1662
                     /rpt_unit_seq="gtttccatacccttctgctcgcgggtctca"
BASE COUNT          508 a          624 c          676 g          543 t
ORIGIN      
        1 cgaaggggtt gtcggtgatg accgactgga cgaggttgtt aaacgccgtt acgctggcga
       61 tcgggaccga aagttcccgg accgccgttc tgtttacagt ggtcggcaca aagtctgcca
      121 tgggttctgt ctcctgcctg ccagggttcc cgggaatgtg acagacatat acagggttgg
      181 cgggagaagg tatcagcctg actgtatcaa aaacgctgga tctggcccgg attcaccaat
      241 ataaccttta ccgtatggct tttcgactca gccgacctat actcccacag gaggatacca
      301 tgaccgccga aaagaccacc cgggagcttg tctacgagac caaccgggac gtgaagtgga
      361 tatgccggac gctccagcag atggaggccc gggacgagga ttttgaggcc cgggtgaggg
      421 cgctggaggg ctggcaggcc gagagagagg gcgcggagcg gcggctctcg acggtgagcg
      481 ccggcgccgg tgggattgtc ggcggggtgg tggcggtgat tttgcgggtg atcggtgggg
      541 gatgaacgaa ggaatggcta tggaatgatg gcttcacaat ctgcgcacat tttggatcag
      601 ggtatgtgac aatcacattc gggggtcgca ttcgattcac ctcgacatac ctgcttcccc
      661 ttccttcccc ctcaagactg atctgcggtt cttcctcctg gagctgctgc atcaaccgga
      721 ctacctgata cctgtccgga tgagtgatct gcctgttctc agcgttagac aggcctatct
      781 ctccacggtc gctgtcgcag cacgctgagc acccgggtct tcgccgcctc ccggtcgatc
      841 cgccgatctc ggtcggggtg gcccggtgcc attagccgcc ggtggatgtc cgggcgaagg
      901 acccagtaag tgccacgccc tgtccagtcc tgatccaggt agccacgcac agtctccatc
      961 tcgtgtgagg cggtcccgcc cctcacgttc ggaacgctgg atcatatgag cgtcgtttag
     1021 gctgtgtgtg tatctattcg tttttgacca gagtgtataa agaaaggctc attccttttt
     1081 gaccacccat gaccaacccc cgcacgcaga tggtggttgg gggggtgcat caagaggtgt
     1141 attccatgac acgattcaaa acgataccca aagtcgtata gtacttgcca gaaccacata
     1201 gcacaacctt acgggggcga gcaagaagag ggtcatccgt ccccccttga ccagtagggg
     1261 taggagacgg tgggaaacag aggtagatcc tggaggcact catgcagatg atccccgcag
     1321 gttgggtcaa accgctccag atggaggaag ggtgtggcat ccggtttcgc ccggttgagc
     1381 aaccggattc tgatggatgg tcaccacaaa cctcctctcg ctcttctcct cctctctccg
     1441 gtcaggaaac agatgatgtc gcggggggcc agagacacac cggataaaac aggtctcacg
     1501 ggcgtggcta tcctttcagg cagaaagagg ggaaagtgac ccgggaaggg attgggtttt
     1561 cacacccttc tgttcgcggg tctcaatgca acaccctagt aagcctatta tattgatcgg
     1621 ctacagtatg gggtttccat acccttctgc tcgcgggtct caatgcaaca cgggccaatt
     1681 acgcccgccg agggggcggt gctggcgttt ccataccctt ctgctcgcgg gtctcaatgc
     1741 aacttgccct tatcggcatt cttatggagg tggcggtcgt ttccataccc ttctgctcgc
     1801 gggtctcaat gcaacgcctt aacctttgag gagcttcctc aggttgagga gagtttccat
     1861 acccttctgc tcgcgggtct cagtgcaaca aaagtccacg ggagaaggga cccgttgggt
     1921 tattcttgtt tccataccct tctgctcgcg ggtctcagtg caacgagaag atgttgatgt
     1981 tgatattagt tggtattaca cttttgtttc catacccttc tgctcgcggg tctcagtgca
     2041 acagtagcac tttcctcgat aaaatccttt ctattggttc ctgcgtgccc caattccagc
     2101 taaaactgcg ggtgtcttcc gcatcacccg gagtataaga agacacccgc aaatcgatct
     2161 caggaaatcg aaaatagggc ccgaaatgac cttcgtttga tcaaaaattg cgggtgtctc
     2221 atgtagaatt gaagtatata aagacacccg caaatcgatc tcaggaaatt gaaaatagag
     2281 gctggaatga accccatttg atcaaaaatt gcgggtgtct ccacagggag aaagcggggt
     2341 gtgatgcata g
//
LOCUS       DAOSZX010000148         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_36243_length_2275_cov_22.068919, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000148 DAOSZX010000000
VERSION     DAOSZX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36243_length_2275_cov_22.068919"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..896)
                     /locus_tag="PK196_08190"
     CDS             complement(387..896)
                     /locus_tag="PK196_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HON41428.1"
                     /translation="MADRILVAYATRYGSTAGVAEAIGDELSKAGEQVDVLPVTEIRD
                     PSPYRAAVIGSPIYMGKWLPEARVFVERYQHYLRTIPVACFAVGLTVAGGSAESLRRA
                     EASMDEVRMLINPVEASVFPGRLDTGRLSDPDRAIIKLIRVKSGDFRNLDAVRSWARA
                     ILPNLHRRR"
     gene            complement(1067..2266)
                     /locus_tag="PK196_08195"
     CDS             complement(1067..2266)
                     /locus_tag="PK196_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON41429.1"
                     /translation="MILTLAARNVRLHFMRSVLAALGIVIGVVAIASIGMVGTNMTLS
                     VTEELSDMANKLTVTAYRGGGGMMGGGPDSGGDEDDEDVITDDEFRDIERIVANYGTA
                     YALMSKSDQIEAGSDTGRATIYGLDPDIIHKTLVVETGKFPESTSSVVVGPTLAERLD
                     LKVGSKIKIGDPDEGPTATVRVVGILEERGMSMDLSTDMAVVGSQKLFTGIYGGEGEY
                     DQVNIVVDDINNVDAVRTAIEDELNRKEDTVTVQDSSRMMESVTSTLSTMTTFVMAIA
                     GISLLVAAVSIFNVMMMSVNERVREIGILRAIGTQRGEVLRMFVYEAGILGIVGAVIG
                     AIASLVIGYLVVFGMVGTADYFFAMDSLIYVPMAMAIGAGICLITGAYPAWKASKLDP
                     IEALRAE"
BASE COUNT          432 a          747 c          625 g          471 t
ORIGIN      
        1 ccccccaggt ggcggctggt cagccgcaaa agacgcttca ccgccgtggt tatggggggg
       61 catcacacca tgagcaaact ggttttatag tccccgtcgc gtaataccgg gaagaaacgg
      121 ggagtttata tcacagaacc gttgatttga tggcctccta tttttcacta cccgccgcct
      181 ccccgattct gtaacccccg tccgtcctcg cgctgcatcc ggctccgcct cccctccttc
      241 cgggggcggg cggccggggc accgtctgga aagtctcccg tttcagatgc tggcctcaca
      301 agaaaagtct caggttcgca ccatctatgg gcccagcgtg cacctggaaa atatgcgcaa
      361 cccaccgcat caggagttcc cgggggtcat ctccgccggt gcaggttcgg gagtatcgcc
      421 cgcgcccagg aacggacagc atccaggttg cggaagtctc cagactttac ccggatcagt
      481 ttgatgatgg cacgatcagg gtcagataga cgcccggtat ccagtcttcc cgggaagacg
      541 ctggcctcca ccgggtttat gagcatcctg acctcgtcca tcgaggcttc cgctcttctc
      601 aggctctcgg cgctcccgcc tgccaccgtg agccctacag cgaaacaggc gacagggatg
      661 gttcgcaggt aatgctggta gcgctcgaca aagacccggg cctccgggag ccatttcccc
      721 atgtagatcg ggctccctat gaccgccgcc cggtaagggg aagggtcgcg gatctcggtc
      781 accggcagga cgtcgacctg ctcaccggcc ttgctgagtt catcaccgat cgcctccgcc
      841 acccccgcgg tcgacccgta tcgggtggcg taggccacaa gaatcctgtc tgccataata
      901 accaggtacc tcttcttccc gtcggactta ttaattgcgg gacgctgccg ggaattatcc
      961 ctgagagcgc cctctgccgc ggagagccca cccatggcgc aacggttcag gggttttccc
     1021 cggcgggaaa accagccctg gactctctcg ccgctgatga tgggctttat tcagcccgca
     1081 gcgcctcgat cgggtcgagt ttcgaggcct tccatgccgg gtaggccccg gtgatcaggc
     1141 agatccctgc acctatggcc atcgccatcg ggacgtagat gaggctatcc attgcgaaga
     1201 agtaatcggc ggtgccgacc atcccgaaga cgacgaggta gccgatgaca aggcttgcga
     1261 ttgcaccgat gaccgcgccg acgataccaa ggatcccggc ctcgtagaca aacatccgca
     1321 ggacctcgcc ccgctgcgtt ccaatcgccc tcaggatacc gatctcacgc acccgttcgt
     1381 tcaccgacat catcatgacg ttgaagatcg agaccgctgc cacaaggagc gagatccccg
     1441 cgatcgccat cacgaacgtt gtcatcgtgg agagtgtcga ggttacgctc tccatcatgc
     1501 ggctgctgtc ctggacggtg accgtatcct ccttgcggtt gagttcatct tcgatcgcgg
     1561 tccttacagc atcgacgttg ttgatatcat caaccactat gttgacctga tcatactcgc
     1621 cctcaccgcc gtagatcccg gtgaagagtt tctgcgaacc gacaacagcc atatccgtgg
     1681 agagatccat tgacatcccc cgctcttcca ggatgccgac gaccctgacg gtcgcggtcg
     1741 gcccctcgtc agggtcgcct atcttaatct tgctccctac cttcaggtca agccgctcgg
     1801 caagcgtcgg gcccacgacc accgaggacg tgctctctgg aaactttccc gtctcgacaa
     1861 ccagcgtctt gtggatgata tccgggtcga gcccgtagat ggttgcccgt ccggtatccg
     1921 accccgcctc gatctgatcg gactttgaca tcagggcata agcggtcccg tagttcgcga
     1981 cgatgcgctc gatatcacgg aactcgtcat cggtgatcac gtcctcatcg tcctcgtcgc
     2041 ctccgctgtc gggccctcct cccatcatac cgccgccgcc gcggtaggcg gtgacggtga
     2101 gtttgtttgc catatcggag agttcttccg tgaccgagag cgtcatgttc gtccccacca
     2161 tcccgatcga ggcgatagca acgacaccga tgactatgcc aagagcggca agcaccgacc
     2221 gcataaagtg aagccggacg ttccttgctg cgagcgtgag gatgatgtct ttttt
//
LOCUS       DAOSZX010000149         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_41268_length_2050_cov_14.448622, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000149 DAOSZX010000000
VERSION     DAOSZX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41268_length_2050_cov_14.448622"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..1667
                     /locus_tag="PK196_08200"
     CDS             558..1667
                     /locus_tag="PK196_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HON41430.1"
                     /translation="MTVAPYVPEPLPPDVIDWEAHIPQIASANRALARYDGILQAIPN
                     PGLLLSPLLTQEAVLSSRIEGTQASLEDVLRFEANPRERINDRTLADIQEIINYREAL
                     NVAVDILKTRPLDTALVCDLHRILLTDSRGMDREPGCVRTIQNFIGRDARVEHAIFIP
                     PAPERVPGALADWEAYLQREEKDVLVQLSVLKAQFELIHPFCDGNGRIGRMLVPLIMY
                     EKGLIGSPMFYISAYLERHRPIYYERLLAVSRERDWNGWITFFLHAIEEQAGANGRKA
                     KAILDLYDEMKRTVPEVTRSQYAVAAIDAIFGTPIFIPSEFYEQTGIPKKTANRILQQ
                     LREHDIIAVLEEGGGRRAATYIFPRLIAITEGDRL"
BASE COUNT          419 a          613 c          634 g          384 t
ORIGIN      
        1 ggggtggcgt ggtctatctg aggccatcga tccaatacgc accccgtttg acaaacctct
       61 tccagaatgg attccaaagg gctgacgtca ccgtgcaccc ctccccatct gggtcaggtg
      121 tggggcgcgg ggttttgtgc ggagatgctc ccggttttca tgcggcatgc ctgcaaagag
      181 acgagcatca tgtggtttcc tggagcacgt caggacaggg gagggagcgt ctccccatca
      241 taccgtgtcc tggatggcgc cctcctcacc cggttcccgg tgaaaactcc ccgaagatcc
      301 tgattccgtc cttgacacgg ccatccgacc aattgcccct cgctgcccgc cccgggaggg
      361 ggggcgggcg ttaaagcatc ctctggtgct caaaagcgcc atcccgggaa caacctggtc
      421 gccggagcaa ccgttgcaat cgcaaacaat caatgttaat actctttcac actaccggat
      481 agttatatga aggatctccg gcacatcgcc agtcttccta ttctcgatgg gttcacggcg
      541 gtccgtgagg aggcggcatg acggttgcgc cctacgtgcc ggaaccgctg ccccctgacg
      601 tgatcgactg ggaagcccat atcccacaga tagcgtcggc gaaccgtgcc ctcgccaggt
      661 acgacggtat tctccaggcg atcccgaacc ccggactcct gctttccccg ctcctgacgc
      721 aggaagcggt gctgtcgtcc cggatcgagg ggacgcaggc gtcgcttgag gatgtcctgc
      781 ggttcgaggc gaacccgagg gagcggatca acgacaggac tcttgccgat atccaggaga
      841 ttatcaacta ccgggaggca ctcaacgttg cggtggacat cctcaagacc cggccgctgg
      901 atacggccct cgtctgcgac ctgcaccgga ttctcctgac cgacagccgg ggcatggacc
      961 gggagccggg gtgcgtccgc acgatccaga acttcatcgg acgggatgcg cgggtagagc
     1021 acgcgatctt catcccgccg gcaccggaaa gggttcctgg ggcgctcgcg gactgggagg
     1081 cctacctgca gcgcgaggag aaggacgtcc tcgtccagct ctccgtcctg aaggcgcagt
     1141 tcgaactcat ccacccgttc tgtgacggca acgggcgtat cgggaggatg ctcgtcccgc
     1201 tgatcatgta cgagaagggg ctgattggaa gcccgatgtt ctacataagc gcctacctcg
     1261 agcggcaccg gccgatctat tacgagcgcc tgctcgcggt ctcccgtgag cgggactgga
     1321 acggctggat aacgttcttc ctccatgcca tcgaggagca ggcgggagca aacggccgga
     1381 aagcaaaggc aatcctcgat ctctacgacg agatgaaacg gacggtgccg gaggtgaccc
     1441 ggtcgcagta cgccgtcgcc gcgatcgatg cgatcttcgg gacgccgatc ttcattccgt
     1501 ccgaatttta cgagcaaacg gggatcccga agaagacggc gaacaggatt ctccagcaac
     1561 tccgcgaaca tgatatcatc gccgtccttg aggagggcgg ggggcggcgg gccgcgacct
     1621 acatcttccc gcgcctgatc gccatcaccg agggagaccg gttgtgaata ttctcgtatc
     1681 ggcaagcctg tttgcggata tccagacccc gctccggtat acacaacggg ccttgcacgt
     1741 atcaggatat ctggaaacct tgacctgggg ccggttgcgg gtcatctgcg atccacaaca
     1801 atatttttgg gtcacaaaat gtattttcat gtcccacaaa acccattgtg ggacaacggt
     1861 gggggacaac ggccggaccc gggtggcggc atgagcacgg gaacagggct gactgaaagg
     1921 gaaccgtatt caacccctgg aaaacagaga tgtatcatgg acgaagagac gggcaggatg
     1981 tttgcgtatg tcagggcggc ggttccggga tcgggagcgc acgggttcga ccataccggg
     2041 agggtcgtgc
//
LOCUS       DAOSZX010000150         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_41375_length_2046_cov_26.625816, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000150 DAOSZX010000000
VERSION     DAOSZX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41375_length_2046_cov_26.625816"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK196_08205"
     CDS             <1..389
                     /locus_tag="PK196_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41431.1"
                     /translation="ASLAIALSCGPDGATFDPPISLTYTLSEDEWARIGEGLRPTVMW
                     YNPESGEWQEIAATVDPATRTITAEVSHFSIFALAWRTPEATPAPTTEATPVPEGEPA
                     AFPVWVIGIVIVLVIAVAAYVLMRRR"
     gene            427..1851
                     /locus_tag="PK196_08210"
     CDS             427..1851
                     /locus_tag="PK196_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HON41432.1"
                     /translation="MIPEHSIMHRAIIALCLLLALAAPAAMAATTELHVVRYAADGVT
                     ILNETTVDYRWLESNLPVQGDGVTHYYHQGPVFEGDKWNPEEDTNVLEKDMGAVKGTD
                     LKDICDLVGGMKEGETVRIKASDGLSRVFPYRNVYEPEPRQGPMVITWYHDEDGYVPD
                     YYSGMRLVFFADTSTNPYGVHAFGVWDMHECFDEEYWYFYGGEYPTTTGLSVQYISEV
                     FIYSNEEPTGSVYVSSIPAGAAIYLDGIDTGLQTNATLEDVPVGEHTIELRLSGYEAA
                     TMNVTVEMDECSRPDPVVLTPLPPEPDATISLKDGWNFISTPKRLMDGSNTFAAIYGS
                     VDTAGHSILLYDGLAQEWKAAASTDAFKPLDGVWVYANETCTVPLTFAPAGPETPPTK
                     NLGEGWNAIGFTDTVPESAANTLLSLGDHWTTLIGFDAGSQEYEVSIVRGATGRHGEE
                     RTMEPMQGYWVYMTGARTLAAIGA"
BASE COUNT          461 a          615 c          586 g          384 t
ORIGIN      
        1 gcgcgagtct cgccatagcc ctttcatgcg gccctgacgg cgcaaccttc gacccgccga
       61 tcagcctcac ctatacactc tcagaggatg agtgggcgag gatcggcgaa gggctgaggc
      121 cgacggtcat gtggtacaac cccgagagcg gggaatggca ggagatcgcg gcgacggttg
      181 atcccgcgac cagaaccatc accgccgagg tctcgcattt cagcatattc gcgcttgcgt
      241 ggagaacccc cgaggccacc ccggcaccga caacggaggc tacacctgta cctgaaggag
      301 agccggctgc gttcccggtc tgggtgatcg gaatcgttat cgtcctcgtc atcgcggttg
      361 cagcatacgt ccttatgagg aggagatagt cagaggattt taattttagt ccaggagagg
      421 agaattgtga tacccgaaca ttcaatcatg caccgggcga tcattgcgct ctgcctgctg
      481 cttgcgctgg cagcacctgc tgccatggca gcgacaaccg aactccacgt cgtcaggtat
      541 gcagcagacg gagtgaccat cttaaacgag acgacggtcg actaccgctg gcttgagtca
      601 aacctccctg tccaggggga cggtgtgaca cactactacc accagggccc cgtattcgag
      661 ggggataagt ggaaccccga agaggataca aacgtccttg aaaaggatat gggcgcggtg
      721 aaggggaccg acctcaagga tatctgcgac cttgtcggcg ggatgaagga gggcgagaca
      781 gtcaggatca aggcatccga cggactctca agggtttttc cctaccgcaa cgtctacgaa
      841 cccgaacccc gccaggggcc gatggtcata acgtggtacc acgacgaaga cggatacgtc
      901 cccgactact acagtgggat gaggcttgtc ttctttgcgg atacctccac aaacccctac
      961 ggtgtccacg ctttcggcgt atgggacatg cacgagtgct ttgacgagga atactggtac
     1021 ttctacggcg gtgagtatcc aacaacaacc gggctctccg tccagtacat aagcgaggtc
     1081 ttcatctaca gcaatgagga gcctaccggg agtgtctatg tatcctcgat cccggccggc
     1141 gcggcgatct accttgacgg tatcgatacg gggcttcaga ccaacgcgac cctggaagat
     1201 gttcctgtgg gtgaacacac gatcgaactc cggttgtcag gatacgaggc tgccaccatg
     1261 aacgtcaccg ttgagatgga tgaatgcagt cgtcccgatc cggttgtgct gacaccgctt
     1321 cctccagaac cagacgccac gatcagcctt aaggatggct ggaacttcat ctccacgccg
     1381 aaacggctca tggatggctc aaacacattc gcggccatct acggcagcgt cgataccgcc
     1441 ggccactcca tcctgctcta tgacgggctc gcacaggagt ggaaagcagc ggcttcgacc
     1501 gatgccttca aaccgctcga tggcgtctgg gtctatgcaa acgagacctg tacagtcccc
     1561 ctgacatttg ctccggccgg acctgaaacc ccgccaacaa agaacctggg cgagggctgg
     1621 aacgcgatcg ggttcaccga caccgttccc gagtccgctg caaacaccct gctctctctg
     1681 ggcgatcact ggacaaccct gatcgggttt gatgcaggaa gccaggaata cgaggtctcg
     1741 atcgtcaggg gcgccaccgg ccggcacggt gaggaacgca cgatggagcc tatgcagggc
     1801 tactgggtct acatgacagg agccaggacg ctagctgcca tcggcgcatg atgctggaaa
     1861 accgggatgc atgcccagaa caccgcccaa ccttttccat gagggggtta gccggcaccc
     1921 ccccttttca tcgagacgct ggagaaagaa catgtcatct gaccactcta tcatcagaca
     1981 ccctgcaatc cttgcgctct gcctgctgct tgcgctggca gcacctgctg ccatggcagc
     2041 gacaac
//
LOCUS       DAOSZX010000151         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_42414_length_2007_cov_16.503074, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000151 DAOSZX010000000
VERSION     DAOSZX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42414_length_2007_cov_16.503074"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK196_08215"
     CDS             complement(<1..674)
                     /locus_tag="PK196_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HON41433.1"
                     /translation="MQRETRVVLLVLLLAGGALYLHTTTTTEEFSRHNIGWNGTSSLP
                     AEEVRDLARLDPGSTLLLLAPDRPFTPEEVGYLRAFLDGGGRVIIADEEGAANPLLAD
                     LGSEIRVRPGNLSSLERDYAHPGLFRGYVVGNDTLFAGVETVLLNRPAEVEGGEPLIE
                     TSVFTWDDVDGDGRVGEGETFGRSVVCAREGNLIVIGDASLFINAMLPENPKFIENLQ
                     PVQIDAV"
     gene            complement(674..>2007)
                     /locus_tag="PK196_08220"
     CDS             complement(674..>2007)
                     /locus_tag="PK196_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HON41434.1"
                     /translation="QFDRLVVTLDLSETDIAEFQKNNRQNQEAMAALLNDSQRLDELQ
                     SLEIEVLDGDQRMAVVYEGEALRQKMKETYTAYAKREPETTRIATHYGANATPYQESV
                     EVLAEVVGAAEEWQVEWQDGDGTSAGGSGGVAPRSPLNITVTPREGRYGENLTIGGRY
                     VDGVPGTPLTIYVDSRIAGNTTLDENAAYAYPYRIGRILAGPHLVYATAGAIFSEVAT
                     FDVLPAETNISLAIAEVNRTAVACTGNLTTEDGRAVLNAPVLLRLDGETLISTQTDGN
                     GTYARTLILTGGEHTVRAEFNAAGYPLNSSRSGVVSIQVRGEGLSPFPFIAAIAAALG
                     AGWYIRRRGLPPEPPVPEEPDIEPPPEIAETGPSVEIEGLPPRDAATILFRALRERLG
                     IPATKTPRECARLAPDHAAFFERYERIRYAGETPTDEELRAMEREALGRDD"
BASE COUNT          336 a          623 c          663 g          385 t
ORIGIN      
        1 acggcgtcga tctggaccgg ctggagattc tctataaact tcgggttctc ggggagcatg
       61 gcattgataa acaagcttgc atcgccgatg actatgaggt taccctccct ggcgcataca
      121 acactccgcc cgaaggtctc gccctcacca acgcggccat caccatcgac gtcatcccag
      181 gtgaagacag atgtctctat gagtggttcg ccaccctcga cctccgcagg acggttgagg
      241 aggacggtct cgacaccggc aaagagggtg tcgttcccga cgacataccc cctgaacaga
      301 ccgggatggg catagtcgcg ctcaaggcta gatagattcc cgggccggac gcggatctcg
      361 cttcccaggt cggcaagtag agggtttgca gccccttcct cgtcggcgat gatgacgcgg
      421 ccaccgccat ccaggaacgc ccgcaggtaa ccgacctctt caggtgtgaa gggccggtcg
      481 ggcgccagga ggaggagggt tgaacccggg tcgaggcgcg cgaggtcgcg gacctcctcc
      541 gcggggagac tggatgtgcc gttccagccg atgttgtgcc ggctgaactc ctcggttgtg
      601 gtggttgtgt gaaggtagag tgcgccgccg gccaggagca ggaccaggag gaccacccgc
      661 gtctcacgct gcatcaatca tccctcccga gcgcctcccg ctccatcgca cggagttctt
      721 catctgtggg tgtctccccg gcgtaacgga tccgttcgta gcgttcgaag aacgcggcat
      781 gatcgggggc gagcctggcg cactcccgcg gcgtctttgt ggcagggatg ccgagcctct
      841 cacggagcgc ccgaaagagg atggttgcgg catcccgcgg cgggagacct tcgatctcca
      901 ccgacggacc cgtttctgcg atttccggcg gcggttcgat atcgggctct tccgggacag
      961 gaggctccgg aggcaggccc cttcgccgga tgtaccaccc ggcaccgagt gcggcggcga
     1021 tggcggcgat gaagggaaac ggcgagagcc cctcgccccg aacctggatg gacacaaccc
     1081 cgctccttga ggagttcaaa ggatagcctg cggcgttgaa ctccgcccgg acggtgtgct
     1141 ccccgccggt aaggatgagc gttcttgcgt aggtgccgtt gccgtcagtc tgtgtgctga
     1201 tgagcgtctc gccgtccagc cggaggagga cgggggcatt caggacagcc cggccatcct
     1261 ccgttgtgag gtttccggtg caggcaacgg ccgtccggtt cacctccgcg atggcgaggc
     1321 tgatattcgt ctctgcaggc aggacatcga atgtcgcgac ctcggagaag atggcgccgg
     1381 cggtggcgta gaccaggtgc ggccctgcca ggatacgacc gatcctgtag ggataggcgt
     1441 aggcggcgtt ctcatcgagg gtggtgtttc cggcgatcct gctgtcgacg tagatcgtga
     1501 gaggagttcc gggaacaccg tccacgtatc tcccgccgat ggtgaggttc tcgccgtaac
     1561 gcccctctcg gggtgtgacg gtgatgttga ggggagaacg gggggcaacc ccgccggaac
     1621 cgcctgcaga ggtcccgtcc ccatcctgcc actccacctg ccactcttcc gcggcgccga
     1681 caacctctgc aaggacctca acgctctcct ggtagggggt ggcgttggcg ccgtaatgcg
     1741 tggcgatcct ggtcgtctcg ggctcacgct tggcgtaggc ggtgtaggtc tctttcatct
     1801 tctgccgcag ggcctcgcct tcgtagacga ccgccatcct ctggtcgccg tccaggacct
     1861 cgatctcgag gctttgaagt tcgtcaagac gctggctgtc gttcaggagc gccgccatgg
     1921 cctcctggtt ctgccggttg ttcttctgga actcggcgat atcggtctct gagaggtcga
     1981 gcgtgacgac aagccggtca aactggc
//
LOCUS       DAOSZX010000152         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_44978_length_1918_cov_14.683306, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000152 DAOSZX010000000
VERSION     DAOSZX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44978_length_1918_cov_14.683306"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..329
                     /locus_tag="PK196_08225"
     CDS             171..329
                     /locus_tag="PK196_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41435.1"
                     /translation="MLQIVNMQQQCLEAEVLKGIIDESGGASLDYFSSKKNVVQSDIK
                     GLAKRSGR"
     gene            complement(710..964)
                     /locus_tag="PK196_08230"
     CDS             complement(710..964)
                     /locus_tag="PK196_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HON41436.1"
                     /translation="MTSLKVEVIDKMAALITAAFGLIAALAWNGAIQELFSIVFGEQS
                     TLVAMFVYAVVVTIIAVIAVVLIGRAAAKAKKADEAGSGH"
     gene            complement(1478..>1918)
                     /locus_tag="PK196_08235"
     CDS             complement(1478..>1918)
                     /locus_tag="PK196_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41437.1"
                     /translation="SVTAFNNLVQSVITDNPFDCVGYTNRDGEYIEPIVLNRERYTAK
                     VNFINDDGKRVGTVSLNSPTIAAFEANAAEVLANTAIATAMGGEAVRDLPNETYYAQL
                     RCHHTSGEDYYLTFTRKTLRLSSYQDEAIRITVEAWADTVGTLD"
BASE COUNT          385 a          534 c          616 g          383 t
ORIGIN      
        1 gcaaaggaga gcccctcttt cggcgtcgct gtaccggaag gggaaggtaa gtcttgagaa
       61 agccgcgttt cttgccggga tgaatataga gtcatacctt gattacctga gaggatgccc
      121 tgatcgggta tgaggagagc ctttcagcga ctgaatttcg gcaaaagagg ttgctgcaga
      181 tagtcaacat gcagcagcag tgcctggagg ctgaagtcct gaagggcatt atcgatgaat
      241 cagggggtgc gtcgctggat tacttttcat cgaagaagaa cgtcgtgcag agcgacatca
      301 agggtcttgc aaaacggagc ggcaggtgac ctatccagat agggatgcag gttctccttc
      361 cttcctggat cgtcatcacc gttcaacccg gggtttatcc tgcggggggg tgaaggggat
      421 gaagcaggaa gccccgtcct ttagggcggg gtagttcacg gagggaagac cgttgtacgt
      481 gaaggacgct ctggcgctcc agcggctcaa ggctgatacg ctccggtcat acctggattt
      541 ccagccggtg atcgataggc acatctccag gatattcggc gattagaatc tacgagcggg
      601 aacgatgagc gattatagag gtggtgatct gcgggcgcaa ggtcaccatc tccggccgag
      661 cgcgggtctt tcaaaaagtg gggccggggg atggacgacg cgccgtcttt tagtgcccgc
      721 tcccggcctc atccgccttc tttgccttcg ccgcagcgcg gccaatcagg acgaccgcaa
      781 tcacagcgat gatagtcaca acgacagcgt agacaaacat cgcaacaagc gtgctctgct
      841 ccccaaagac gatactgaag agttcctgaa tggcgccgtt ccaggcaaga gcggcgatca
      901 gaccgaacgc cgccgtgata agggcggcca tcttatcgat gacctcaacc ttcagagacg
      961 taataaacca ccgacgagag ggtgcacttt ttgcctgata accctttctg tgtatgctcg
     1021 gggggttacg agcgggacaa cccggagtga cacccccggc acggtatcgg cgtgtcagcg
     1081 ggaacggtgt tcgccgagcc acccccgccg cccggccgct ttgcggcctc ctcccgcccc
     1141 cttcccgagg ggcgggcagt atgtggcgat agcaccggat agccgtgcac ggggagagga
     1201 ttcttccggg gattctcacc tgaaaccggg gtgacgcgat gcacaccccc ggcactgtat
     1261 cggtgggata tcgcccccgc ggggcggggg cggaacgccc cttcccctgt cgctcgcgtc
     1321 tcacccgttg agcggtttac gtagagcacg aacgggatct cgctgagggt gtttacatcg
     1381 acggtagagg gacgatcact ccgccccgcc agaatagagt aacgataaag gagggtggag
     1441 cgtggggggc caaaaacccc gttccttttc cctcttctca gtccagcgtc ccgacggtgt
     1501 cggcccaggc ctcgaccgtg atcctgatgg cctcatcctg gtaggacgag agcctgaggg
     1561 tcttccgggt gaaggtgagg tagtagtcct cgcccgaggt gtggtggcac ctgagctggg
     1621 cgtagtaggt ctcgttcggg aggtcgcgga cagcctcgcc gcccatcgcg gttgcaatgg
     1681 cggtgttcgc aagcacctcg gcggcgttcg cctcgaaggc tgcgatcgtc ggcgagttga
     1741 gagagaccgt cccgacacgc ttcccatcat cgttgatgaa gttcaccttc gcggtgtagc
     1801 gctcgcggtt caggacgatc ggctcgatat actccccgtc gcggttggtg tagccgacac
     1861 agtcgaaggg gttgtcggtg atgaccgact ggacgaggtt gttaaacgcc gttacgct
//
LOCUS       DAOSZX010000153         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_45668_length_1895_cov_21.120109, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000153 DAOSZX010000000
VERSION     DAOSZX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45668_length_1895_cov_21.120109"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1895
                     /locus_tag="PK196_08240"
     CDS             136..>1895
                     /locus_tag="PK196_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON41438.1"
                     /translation="MVRYSVTMDDDLVQEIDSVREYHGMSRSEWLNNLCRKYLSSTPA
                     NGVKTDVPTGVPSGNTREPNMTDYEAACANFRIDVPEYFNFGYDVIDRWAEIDRNKLA
                     MIWVNHAGEEKKYTFRDLKHLSNGAANILLKYGIKKGDRVMLMLPRIPEWWIFVLALI
                     KLGVVFCPCPTMLTPKDIKYRVQAGKFRMIITDSENADKVDQVADACHMLDTLFLVDG
                     EREGWASYPHELEYPAPVSHHTVSMPVKKKTRSTDPMMIYFTSGTTGEPKMVLHNHAY
                     PLGHIVTASLWHDVTANDLHFTFSDTGWAKCAWGKIFGQWIAGACIFVYDARGKFSAT
                     ELLPMIEKYEVTTFCAPPTVYRMLILADLDRFDFRELRHCCSAGEPLNPEVIRVWKDG
                     TDRLIYEGYGQTETVCCIATYPCMEHRPGSMGKPAPGWHIELHDDDGNPVGVREEGRI
                     AVRLDPRPVGLFVEYLDNPEANRESFKNGFYYTGDKAYMDEDGYFWFVGRDDDVIKSS
                     GYRIGPFEVESALMEHPAVQEAAVVGSPDLIRGMVVKAFVILKPGYQPSESLVRELQA
                     HVRKTTAPYKYPRLIEFVDSL"
BASE COUNT          431 a          586 c          548 g          330 t
ORIGIN      
        1 ggggatgggg acgggacgcc ggtgcgtcct ttgaccatac ccgcaccgtg ctgcaccatg
       61 gaatgcccgg atttgcaaac agaggtgcac cagagatcaa aaaggtgata tgccgcacca
      121 gaaaacctgt atggtatggt gcgatactcg gtcacgatgg atgacgacct cgtgcaggaa
      181 atcgacagcg tacgcgaata ccatggcatg tccagatctg aatggctcaa caacctctgc
      241 aggaagtacc tcagcagcac cccggcaaac ggcgtgaaaa ccgacgtccc cacaggtgtc
      301 ccgtccggga acacccggga accgaacatg accgattacg aggcggcctg cgccaacttc
      361 aggatcgatg tccccgagta ctttaacttc ggctacgacg tgatcgaccg gtgggccgag
      421 atcgaccgga acaaactcgc catgatctgg gtgaaccatg caggagaaga gaagaagtac
      481 accttccgcg acctcaaaca cctctccaac ggcgccgcaa acatcctgct gaagtacggg
      541 atcaagaagg gagacagggt catgctgatg ctcccccgga tccccgagtg gtggatcttc
      601 gttctcgcgc tcatcaaact cggcgtcgtc ttctgcccct gccccacgat gctgacgcca
      661 aaagacatca agtacagggt gcaggccggg aagttcagga tgatcatcac cgacagcgag
      721 aatgccgata aggtcgacca ggtcgccgac gcatgccaca tgctcgacac cctcttcctt
      781 gtggacggcg aacgcgaagg ctgggcaagt tacccccacg aactcgagta tcctgcaccg
      841 gtatcgcacc acaccgtcag catgccggtc aagaagaaaa cgagatcgac cgacccgatg
      901 atgatctact tcacctccgg caccaccggc gagcccaaga tggtgcttca caaccacgcc
      961 taccccctcg ggcacatcgt caccgcctcg ctctggcatg atgtcacggc aaacgatctc
     1021 cacttcacat tctccgatac cgggtgggca aagtgcgcat ggggcaagat cttcgggcag
     1081 tggatcgccg gcgcctgtat atttgtttat gatgcccggg ggaagttctc ggcgacagaa
     1141 ctcctcccga tgatcgagaa gtacgaggtg acaaccttct gcgcaccgcc gacggtctac
     1201 cggatgctga tcctggcaga ccttgacagg tttgacttcc gcgaactgcg gcactgctgc
     1261 agcgccggag aacccctcaa ccccgaggtt atccgggtct ggaaagacgg cacagaccgg
     1321 ttaatctacg agggttacgg ccagacggag accgtctgct gcatcgccac atatccctgc
     1381 atggagcaca ggcccggttc tatggggaaa ccggcgccgg gctggcatat cgaactccac
     1441 gacgacgacg gcaaccctgt cggagtcagg gaggaggggc ggatcgcggt caggctcgac
     1501 ccgcggccgg tagggctctt cgtcgagtac ctcgacaacc cggaggcaaa ccgtgagtcg
     1561 ttcaagaacg ggttctacta caccggcgac aaggcctaca tggacgaaga cggctacttc
     1621 tggttcgtcg ggcgtgacga cgacgtcatc aagtcgtccg gctacaggat cgggccgttc
     1681 gaggtcgaga gcgcccttat ggagcacccc gccgtccagg aggcggcggt tgtcgggtcg
     1741 cccgacctca tccgcgggat ggtggtaaag gcgttcgtca tactgaaacc cggttatcag
     1801 ccatcagagt cgctggtccg ggaactccag gcccatgtcc ggaagaccac cgccccctac
     1861 aagtaccccc ggttgatcga gttcgttgac tcgct
//
LOCUS       DAOSZX010000154         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_45877_length_1888_cov_15.179487, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000154 DAOSZX010000000
VERSION     DAOSZX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45877_length_1888_cov_15.179487"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK196_08245"
     CDS             complement(<1..214)
                     /locus_tag="PK196_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41439.1"
                     /translation="MTKQPSKRTLTALLGLAVFLTLSVPCSLLIVLGTGGPGALAAAL
                     TLLLGGILILLILQRYRKYRYRPGPAL"
     gene            complement(587..1432)
                     /locus_tag="PK196_08250"
     CDS             complement(587..1432)
                     /locus_tag="PK196_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HON41440.1"
                     /translation="MFTARRYHQRDHTPSTSTSTRSGRLLRLLILSILFSVLLVSVAD
                     ATDYTVRPSRNVGQEPGMSVSGEKVQEIDPIPFWLFLLVSVFPQLTAAPIETLLPLKT
                     VGYLGYKRICKENALESPQRRKILRFVKRNPGLHFREILRRVPLTRGTLDHHMKTMES
                     VGLLKTVSGRGRVHYFVADSSYSAEEEILITVLRNESLRRIITEIYLNQGACNEEIAE
                     DVGLSGATVYEHLRYLKDLGVVIAERDGRCVRYTLAESYSRILAGMRIIIPNRPGMRL
                     IPPDR"
     gene            complement(1371..>1888)
                     /locus_tag="PK196_08255"
     CDS             complement(1371..>1888)
                     /locus_tag="PK196_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41441.1"
                     /translation="DENRIGVCHIKLIKRGTQPSFMVSAKRKCMAFALLLAVAFLAVP
                     PVSANEGVGYKVRPSVSDDPVTTLGLSDTVGQGETNRHQFYVGSEVAYLEMYLNWGST
                     SDSLALTIYTPSGSTIGTFRDSSDGMTDGKIHIDIDPDGNYVDQETWTLDVYGEKVSS
                     ERSYTLNLYQH"
BASE COUNT          406 a          523 c          529 g          430 t
ORIGIN      
        1 gcagagcggg gcccggccga tacctgtact tccggtatct ctggaggatc aggagtatga
       61 gaatccctcc caggaggagg gtgagcgctg ctgccagggc accaggaccc ccggttccga
      121 gaacgatgag gagactgcac gggaccgaga gggtaaggaa gacggccagg cccaggaggg
      181 cggtaagggt tcttttgctg ggttgttttg tcatggttag ttatcactta cccggcagcc
      241 ctatcgccct ccgggcacgc ggtgggagtc cgggatccga cgccaatcga gacacccggc
      301 cccctcggga taccgcgtgc ggcttccccg atggagactt cgctgtcctt ctttataccg
      361 tttctcggac aaacgccgat tcatccgggc gctcagagac ctcaagttca gggaaaaaga
      421 gcaggagtgc aggtatcgga acctgatact ccggtgcttc ccccaaaaga ctggtgtgca
      481 gcagggtgcg cactcggggc cgggcgtcac gaggaatgat cgccaacgtg ggagtccggg
      541 tcaaacctca gttgagagat gaggccgctg atatcactcc ccggggctat ctatccggcg
      601 ggatcagccg cattccgggc cggttcggta taatgatccg catacccgcc agtatacgag
      661 aataactctc ggcgagggta taccgcacac accgcccgtc cctctcagcg atcacaacac
      721 ccagatcttt caggtatcgt agatgttcat aaactgtagc tccggatagt ccgacgtctt
      781 ctgcaatttc ttcgttgcat gcaccttgat tgagatagat ctctgttata attctccgca
      841 gactctcatt cctcagtacg gtgataagga tctcttcctc ggccgaatat gatgaatctg
      901 cgacgaaata atgaaccctc cctcttcccg ataccgtctt taagaggccc acggattcca
      961 ttgttttcat atggtgatca agcgtccccc gcgtcagagg tacacgcctc aatatctccc
     1021 tgaagtgcaa tccagggttc ctcttgacaa atctcaagat cttgcgtctc tgcggactct
     1081 caagagcgtt ttctttgcag atccgcttgt acccgagata tccgacggtc ttcaggggaa
     1141 gaagggtctc gatcggtgct gccgtcagct gggggaatac ggataccagc aggaagagcc
     1201 agaacggtat tggatctatc tcctgcacct tctcgcccga tacggacatg ccgggctctt
     1261 gcccgacgtt tcttgagggc cggaccgtgt aatcggtagc atcggctacc gataccagca
     1321 gcaccgaaaa gaggatcgag aggatgagca gccggaggag tctgcccgat ctagtgctgg
     1381 tagaggttga gggtgtatga tctctctgat gataccttct cgccgtaaac atcgagcgtc
     1441 caggtttcct ggtccacata attgccatct gggtcaatgt cgatatgaat cttgccatcc
     1501 gtcattccgt cgctactgtc gcggaacgtg ccgatcgtgc ttcccgaagg ggtgtagatg
     1561 gtcaacgcca gagaatcgga ggttgaaccc caattcaggt acatttcaag gtatgcgacc
     1621 tcactcccga cataaaattg gtgcctgttg gtctcgccct ggccgacggt gtcggagagt
     1681 cctagcgtcg tcaccgggtc gtccgacacc gaagggcgga ccttgtatcc gacgccttcg
     1741 ttcgccgata caggaggcac ggcaaggaag gcaacggcga gcagcagggc aaaagccatg
     1801 cattttctct ttgctgatac catgaaggag ggttgtgttc cgcgttttat gagttttatg
     1861 tggcaaacac cgattctatt ctcatctc
//
LOCUS       DAOSZX010000155         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_46888_length_1856_cov_18.573570, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000155 DAOSZX010000000
VERSION     DAOSZX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46888_length_1856_cov_18.573570"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="PK196_08260"
     CDS             <1..1719
                     /locus_tag="PK196_08260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844956.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HON41442.1"
                     /translation="PVEPIEKGILRALQNLYVFKDGTVRYDMIDLPLTHFRPGEIGVP
                     IGRLHELGYTRDIHGQELASDDQVLELRHQDILVSEDCGEWLVRVSKFIDELLVKFYG
                     LEPFYNAEKPSDLVGHLLIGLAPHTSAGVLARLIGFSKAPVGYGHPFFHAAKRRNCFA
                     GDTEITVSDGRRWLTMPIRQFVVENFDISRPGLDHLGTYYSDPKQPFYVRSIDTQGKV
                     SLKRVTSVSVHRAPAHMIRFETRRGKFITVTPDHAMLVWDTDYLRKVRALEAAPGDRV
                     PVEEGGVVVSDEVLARETVQALDDRVYCLTVADNHTLAANGIFCGQCDGDEDCVMLLL
                     DGLINFSRSYLPETRGGTMDAPLVLTTRIDPAEVDKECHNVDVCDHYPLEVYNGCLAY
                     THPKDLEDHVDRVERRIGTPAQFEGFSFTHPTSNISAGPLESTYTKLGSMLEKLEAEL
                     ELAKKIRAVDEDDVAERVLNTHFIRDLQGNLNAFSKQKVRCMKCNAKYRRMPLAGKCT
                     RCGGNLIPTVHEGSVKKYLEMSRNICANYRVSDYTKQRVEVLAMQIESTFGEPPERQL
                     GLADFM"
BASE COUNT          373 a          560 c          581 g          342 t
ORIGIN      
        1 ccggtggaac cgatcgagaa ggggatcctc cgggcgttgc aaaaccttta tgtattcaag
       61 gacggcactg tccgctacga tatgatcgac ctccccctca cccacttccg gccgggtgag
      121 atcggtgtcc ccatcgggcg gctccacgaa ctcggctaca cccgggacat ccacggccag
      181 gaacttgcct cggacgatca ggtgctggaa ctccgtcacc aggatatcct ggtctcggag
      241 gactgtggcg agtggcttgt ccgggtgtca aagttcatcg acgaactcct ggtgaagttt
      301 tacgggcttg aaccgtttta caatgcggag aaaccctctg acctggtcgg ccacctgctg
      361 atagggcttg ccccgcacac gagcgccggg gttctcgcca ggctgatcgg gttctcgaag
      421 gcgccggtgg gctacggcca cccgttcttc cacgcggcga agaggaggaa ctgttttgcc
      481 ggcgataccg agattaccgt ctcagacggg cggcggtggc tgacgatgcc catacggcag
      541 ttcgtcgtgg agaacttcga tatctcaagg ccggggcttg accatctcgg gacctactac
      601 tccgacccga aacaaccgtt ctatgtgaga agcatagaca ctcaggggaa agtcagcctg
      661 aaacgggtga cctcggtctc ggtccaccgc gcccctgcgc acatgatccg gtttgagacc
      721 cggagaggga agtttatcac ggtcacacca gaccacgcga tgctcgtctg ggacacggat
      781 tatctccgga aggtccgggc gctggaggcc gcacccggcg accgcgtccc ggtggaggag
      841 gggggggttg tcgtcagcga tgaggttctg gcccgcgaga cggtgcaggc gctcgatgat
      901 agggtatact gtctcacggt agccgataac catacccttg ccgcaaacgg gatcttctgc
      961 gggcagtgcg acggtgacga ggactgcgtg atgctcctcc tcgacgggtt gatcaacttc
     1021 tcgcgctcct acctcccgga gacccggggc gggacgatgg acgccccgct cgtcctgacg
     1081 acacggatcg acccggcaga ggtcgataag gagtgccaca acgtcgatgt ctgcgaccat
     1141 taccccctgg aggtctacaa cggctgcctc gcatacaccc acccaaaaga ccttgaagac
     1201 cacgtcgacc gggtggaacg gcggatcggc accccggccc agtttgaggg gttctccttc
     1261 acccacccga cctcgaacat ctcggcgggg cctctcgagt ccacctacac taaactgggg
     1321 tcgatgcttg agaaactgga ggcggaactc gaactcgcga agaagatccg ggcggtggat
     1381 gaggacgatg tcgctgaacg tgtcctgaac actcatttca tccgcgacct ccaggggaac
     1441 ctcaacgctt tttcaaagca gaaggtgcgg tgcatgaagt gcaacgcgaa gtacaggcgg
     1501 atgccgcttg ccgggaagtg cacccgttgc ggcggaaacc tcatccccac cgttcacgag
     1561 ggttcggtga agaagtacct ggagatgtcg cgaaacatct gtgcaaacta ccgggtgtct
     1621 gattacacaa aacagcgggt ggaggtgctc gcgatgcaga tcgagtccac cttcggcgag
     1681 ccccccgagc ggcagctggg gcttgcggac tttatgtgag ggtgaggctg cgtgacaacg
     1741 ctcccgcccg cggccgtcct ggctgctctg agatccggga cgcccatccc gacggaggaa
     1801 tccagggtgc cctggattga gcggctggga tgcggcacca cggcataccg tttaac
//
LOCUS       DAOSZX010000156         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_47681_length_1832_cov_17.651097, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000156 DAOSZX010000000
VERSION     DAOSZX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47681_length_1832_cov_17.651097"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PK196_08265"
     CDS             <1..1497
                     /locus_tag="PK196_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HON41443.1"
                     /translation="LRRCATIDAIALRACRAEGLIDRSPDQIIVPGVKRAIPVYQEFM
                     QHHGIPYSPVARLADDGNPVARAGLPAGNQILEIGTYLTATMRPPDAWREAASALGLS
                     FNGSAWGITDLLSAWQEFKAAKGVFEHPDYVRLAVDSNVEPPAPVIFVDEYQDVSPLQ
                     DALIRQWIEKVDRVYVAGDPDQAIYGFRGCSPDLFLSLPAADRDNGSRPVSHRCPIRV
                     METAENVLGHPANVAPNPRPGISLRCTFPDREALVRQVEEALRTYTDTVTPVYVLCRF
                     QRHVSKIMRDLSSAGLPCTGIRDVDGGPWGMVYIGRHDKRLERDRVNVWSLVCALRRL
                     QGNPLPIEPLPLDEAEALVAGAVRSSRRRSQAISALKTKATQHPPIRVGDITEWVETP
                     IKVDALNLRPAIVEQIRNCLAREQRRGVVIHPQAVRVDTIHSAKGLEAPCVLLHTGYL
                     HGLCDALVAPDRMAEERRVFFVGVTRASRALILFDYISPVWPGFGRPV"
     gene            complement(1618..>1832)
                     /locus_tag="PK196_08270"
     CDS             complement(1618..>1832)
                     /locus_tag="PK196_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41444.1"
                     /translation="RRRPEKSRLHLFSFRHVVYYHHFHNNSTVPTKYLLEVRGLRVSF
                     SSSRREEEGHADHSHRRHRHHRRVTG"
BASE COUNT          353 a          553 c          560 g          366 t
ORIGIN      
        1 ctccgccggt gtgcgacgat cgatgccatt gctctccgtg cctgccgggc ggagggttta
       61 atcgaccggt cacccgacca gatcattgtg ccgggggtga aacgcgcgat cccggtatac
      121 caggagttca tgcagcatca tggaattccg tattcaccgg ttgcacgtct cgccgatgac
      181 ggcaacccgg tagcgcgtgc cggtctcccg gccggcaacc agatcctgga gatcgggaca
      241 tacttaaccg caaccatgcg cccgccggat gcctggcggg aggcggcttc ggcactgggg
      301 ttatcattca acgggtcggc atggggtatc accgacctcc tctccgcgtg gcaggagttt
      361 aaggcggcga aaggtgtttt cgaacatcca gactacgtac ggcttgccgt tgattcaaat
      421 gtggaacctc ccgccccggt tatcttcgtt gacgagtatc aggatgtcag cccgttacag
      481 gacgctctga tccggcagtg gatagagaag gttgaccggg tctatgtcgc gggcgacccg
      541 gaccaggcga tatacgggtt ccgcggatgc agccccgacc tgttcctctc cctgccggca
      601 gcggaccgtg ataacgggtc ccggccggta tctcaccggt gccctatcag agtcatggaa
      661 acagcggaaa acgttctggg tcacccggca aacgtggctc caaacccccg accaggcatc
      721 tctctgcggt gcacgttccc ggaccgcgag gcgcttgtgc gacaggtcga agaagcgctg
      781 agaacataca ccgataccgt tactcctgtt tatgtgctct gtaggttcca gcggcacgtt
      841 tcgaaaataa tgcgcgatct atcctccgcc ggtttaccgt gcaccgggat ccgagacgtt
      901 gacggcggac cgtgggggat ggtctacatc ggccggcacg ataaacggct tgaacgtgac
      961 cgcgtgaacg tctggtcttt ggtgtgtgca cttcgccggt tgcaaggcaa cccccttccg
     1021 atagaaccgc tcccgttaga cgaggcggag gcgcttgtgg ctggtgccgt gcggtcatcc
     1081 aggagacgct cacaggcaat atccgccctg aaaaccaaag caacccaaca ccccccgatc
     1141 cgggtgggtg acatcaccga gtgggtcgag acacccatca aggtcgatgc cctgaatctc
     1201 cggccggcga tagtggagca gatacggaac tgtctggccc gcgaacaacg gcgaggggtt
     1261 gtgatccatc cccaggcggt ccgggtggat accatccatt cggcaaaagg ccttgaggcc
     1321 ccgtgtgtgc tcctacacac cggttatctt cacggtctat gtgacgctct ggtcgccccg
     1381 gaccgtatgg ccgaagagcg gcgggtcttt ttcgtgggtg tgacgcgagc atctcgtgca
     1441 ttgatcctct ttgactacat atcaccggtc tggcccgggt tcggccggcc ggtatgacct
     1501 ccctcctcgc cggcggcaac ataataagag gcacacctca cagacaaaac cccgttcgcc
     1561 gggtgagaaa cggttttgtg ctcataaatg ttgttccacg tataccccac atgcccctca
     1621 gccggtgacc cggcggtggt ggcggtgccg gcggtgcgag tggtccgcgt gaccctcctc
     1681 ctcccgccgc gacgaactga aggagacacg aaggcccctc acctctaata ggtacttggt
     1741 ggggacagta gagttgttgt ggaagtggtg gtagtagaca acgtgccgaa agctgaagag
     1801 gtggagacgt gacttctccg gcctccgccg cg
//
LOCUS       DAOSZX010000157         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_48567_length_1808_cov_26.636623, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000157 DAOSZX010000000
VERSION     DAOSZX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48567_length_1808_cov_26.636623"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..>1808
                     /locus_tag="PK196_08275"
     CDS             364..>1808
                     /locus_tag="PK196_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HON41445.1"
                     /translation="MPDEAVWEMQRSGATGRVEGHGSIVDTAAGEADHPQRRILNRIL
                     RPGEEKRRTGEYDPAKSGPLTEALIPPGYREIDRYWVTEGIALVVIARNLDTKQTEYL
                     LFEPVLSEFEYELLERLYADLRDVLILEDRDLVTDRRVVLAKRVENLLAEYGITLPDD
                     SFYRLRYYLERNFIGWSRIDALMKDPRIEDISCNGANIPIFLYHRKHQNIKTHISFDE
                     PALNSLAIKLAQRSGKHVSIGNPLVDATLPDGSRLQVALGREVTSRGTSFTIRKFRES
                     PFTPVELMETNTFDIDQLVYFWLAIENNKSLLFVGGTASGKTTSLNAVALFIPPLSKI
                     VSIEDTREITLYHENWVATVTRETTAGAPGATPIGMFELLKAAMRQRPEYILVGEVRG
                     SEAQTLFQAMNTGHTTFSTMHASDVDAAIHRLESAPLNVPRNMLQALDIISVQALTHR
                     GGDRVRRCREIVEIAGVDPNTGNVQVNTVFD"
BASE COUNT          418 a          545 c          498 g          347 t
ORIGIN      
        1 agatcgtgga gaggcttgaa ctgaacatat tccctaatat atgaggagat tgggtatcag
       61 gtgagaccgc gaagtagtgg tctcctcccg tggcactgtg tctatgggag aatcgccccc
      121 gtgggggagg ggctgacggg gaggggggag gcggatacgg ctcccccctc ccatgtctcc
      181 tgcgtctctg gttaacggta ttcatagact acccccacct cccggccacc ccccggaatc
      241 tgtccgtgga ctggacacgg agaggtttat caaaccagtg tggctgttgg tttgatggcc
      301 tccaccaatt ctctgctgga aaaaccgcaa atttatatgt ctgctccacc acatccccta
      361 ctgatgccgg atgaggcggt ctgggagatg cagagatctg gtgcaacagg gcgtgtagag
      421 gggcacggtt ctatcgtgga cacagcagct ggggaagcgg accaccctca acgccgcatc
      481 ctcaaccgca ttctccggcc aggggaagaa aaacggagaa cgggtgagta tgatccggca
      541 aaatccggcc ccctcactga agcgttgatc ccgccgggct acagagagat agaccgctac
      601 tgggtcacag aaggtatagc actcgtcgtg atagcccgca acctcgatac gaaacagact
      661 gagtacctct tattcgaacc ggtgctctcg gaattcgagt acgaactcct ggaacgcctc
      721 tacgcagacc ttagagatgt cctgatcctg gaagaccgcg acctcgtcac cgaccgtcgg
      781 gtcgttctgg caaagagggt ggagaacctg ctcgctgagt acggcatcac cctcccggac
      841 gactcattct accggctccg ctactacctt gaacgaaact tcattggctg gtcgcgcata
      901 gacgcgctga tgaaggatcc aagaatcgag gatatatcct gcaacggcgc aaacataccc
      961 atattcctct atcaccgcaa acaccagaac atcaagaccc atatcagttt cgacgaaccc
     1021 gccctgaact cccttgcgat caaacttgca cagcgttctg gaaagcacgt ctccataggt
     1081 aacccgcttg tggacgcaac cctgccggac ggctcacggc tgcaggttgc actcggccgt
     1141 gaggtcacca gtcgcgggac atcgtttacc atccgcaagt tccgcgagtc gccgttcaca
     1201 cccgtggaac tcatggagac aaacaccttc gacatagacc agctcgtcta cttctggctg
     1261 gcgatcgaga acaacaaaag ccttctcttc gtcggcggga cggcgtcggg taagaccacc
     1321 tcgttaaacg cagtcgccct ctttatcccc ccgctctcaa agatcgtctc catcgaggac
     1381 accagggaga tcacactata tcacgagaac tgggtcgcga ccgtcacgcg cgagacgacc
     1441 gcgggggcgc ctggcgccac tcctatcgga atgtttgaac tcctgaaagc cgcgatgcgc
     1501 cagcgtcccg agtacatcct ggtcggggag gtccgcggga gcgaggccca gacactcttt
     1561 caggcgatga acacggggca cacgacgttt tccacgatgc acgcaagcga cgtcgatgca
     1621 gcaatccacc gcctggagag cgcgccgctc aatgtaccga gaaacatgct ccaggctctg
     1681 gacatcatat cagttcaggc tctcacccac cgtggaggcg atcgcgtgag aaggtgccgc
     1741 gagatcgtcg agatcgccgg ggttgacccg aacacgggga acgttcaggt aaacaccgtc
     1801 tttgatta
//
LOCUS       DAOSZX010000158         1805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_48670_length_1805_cov_16.022857, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000158 DAOSZX010000000
VERSION     DAOSZX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48670_length_1805_cov_16.022857"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            549..800
                     /locus_tag="PK196_08280"
     CDS             549..800
                     /locus_tag="PK196_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41446.1"
                     /translation="MNDAFLANAAEVLANTAIAAAMGGDVKRDLPHETYYAQLRRHHT
                     SGEDYYLTFTRKTLRLSSYQDEAIRSTVEDWADAVGTLD"
BASE COUNT          381 a          582 c          529 g          313 t
ORIGIN      
        1 tccagtcagg ggaggtctat gccggatccc gaaccgggaa gagattctta agtgtagatg
       61 aaaaaagccc gcggccagat ggttcgagga gagagagcag tcaccctgtg ctcctgcagg
      121 cgtcttaatc ccggacaccg catgattcat ggaccatgct cttcatcccc tcctcctttt
      181 cacatcccat ctctaaaccg caagatccgt atacctgcaa tcccttgcag catctcgtat
      241 agaagacagg gtttctgcct ggctgccaat cgatagcagc gttttctctt tcaagacggt
      301 cgatctcctc agcctgtgtg accccatcac tgtctgtctc ctgccgggca gccgcgaccc
      361 ctgccgcccg gcggaatccg ggaccggatc gagccgcaga tcaaacacct cagagaaggc
      421 cgggagcggc ctcacgaaat gtgggtttga cccggtagaa aggccggcag gagaggcttc
      481 ccccctttgt ccggagacat tctcacagat gcgtcccaca agaggtctgg agccgcgagc
      541 agcccgggat gaacgatgcg ttccttgcga acgccgccga ggtgcttgca aacaccgcca
      601 ttgcggccgc gatgggcggc gatgtcaagc gcgacctccc gcacgagact tactacgccc
      661 agctcaggcg ccaccacacc tcgggcgagg actactacct caccttcacc cggaagaccc
      721 tcaggctctc gtcctaccag gatgaggcca tcaggagcac ggtcgaggac tgggccgacg
      781 ccgtcgggac gctggactga gaagatggaa aaggaacggg gtttttaccc caccttccac
      841 cctattttat tgcaactcta ttctggaggg gcggggtgat cgtccataga tacggttcca
      901 ggggagagca tcaacccccc tgccggtgga tccccccgcg tcaagatccc gctcctgctc
      961 cccggacacg gctatcgggt gctatcgcca aacactcgcc cgcggcagcg gccgggaggt
     1021 ggggggtgga cccagaggtt aaaacaccgc cacaggagac gcaagcgaca ggggaagggg
     1081 cgttccgccc cctccccggc gagccccacc cccacggggg cgatatccca tcgatacagt
     1141 gtcccggcca ggatgagcag atccgcggct cgatgtgaac acccttgact gaaatcctgt
     1201 tcctgctcac catgcacggt atccggtgct atcgccactc actgcccgcc cccctggagg
     1261 gggcgggagg aggccgcgga gcggccggga ggcgggggtg tttcacgaat accgttcaac
     1321 cggagacata ggagacaggg gcgggggaaa gtttctttcc cctaccccgg cgggggcgat
     1381 tctcccttga tacactgcta gggaggagac cactcctctg cagtcaacgt gaacaccctt
     1441 gactgaaatc ctgcttccgt ccccggacac ggtatccggg ccaatcgcca tacacttgcc
     1501 cgcggcagcg gccgggaggt gggggttgtt caacgaacac tcatcagcca gagatgtaag
     1561 gaagagacag agacctgggt gttcaacgaa cacccctcaa caagacacaa gaccggcccg
     1621 ccgccggccg ggggggtggg cgcgcccaag aggatctttc cccggcgtct tggaaacaat
     1681 gcagttgcaa cgttgcgtgg aacagtgagc gcccaaggat ctgcgtccgc ccagaaccct
     1741 cctccgggtt tcacacaggc gggaaaaaag attgggggaa aaatcccctc tctcactccc
     1801 tctcg
//
LOCUS       DAOSZX010000159         1791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_49144_length_1791_cov_18.500000, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000159 DAOSZX010000000
VERSION     DAOSZX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49144_length_1791_cov_18.500000"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1613
                     /gene="serA"
                     /locus_tag="PK196_08285"
     CDS             30..1613
                     /gene="serA"
                     /locus_tag="PK196_08285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HON41447.1"
                     /translation="MRYRVLVSDPLAEEGLDILKEFCDLDIKTGLTEDQLVDIIGDYD
                     ALLVRSGTEVTARVIEAGKRLKFIGRAGAGVDNIDTEAATRKGIIVANAPEGNTLAAT
                     EHTMAMMLALARNIPQANASVKKGEWKRSKFMGVELNEKVLGIVGFGRIGREVAKRAQ
                     AMEMKCIAYDPFISSERAASLGVEMVTLEDLFRRADVITVHTPLIKETHHMINAGTIA
                     TMKDGVRLINCARGGIIDEKALADGIASGKVAGAALDVFESEPPTDSPVIGLEQVIVT
                     PHLGASTVEAQKNVAISIANQCISVLSGGSAKYVVNAPVVPMEQQALIEPYARLAQKM
                     GRLLIQLIEGRLEAIDVTYGGEAANLPNTKFITRVILKGMLDPILQVPVNLVNAEFVA
                     KERGIRLSETTTEEAQGFRNIITITARTDRMTETVSGSVSSPNRARIVSIGGYMTDLT
                     PTGHVVISRHTDKPGVIGKVATILGRVNVNIAGMQVGRHRPGEEALMVLTVDSPVPPE
                     AMEEIKRVDGIHTAKYAEI"
     gene            1718..1791
                     /locus_tag="PK196_08290"
     tRNA            1718..1791
                     /locus_tag="PK196_08290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Val,seq:gac)
BASE COUNT          378 a          447 c          632 g          334 t
ORIGIN      
        1 tatgatgttg aattagtatg ggttgaaccg tgagatatag ggtactggtc agcgatccgc
       61 tggcagagga agggcttgat atcctgaaag agttttgtga tctggatatc aaaacggggc
      121 tgactgagga tcagctcgtc gatatcatcg gtgattacga tgccctgctg gtgcgctcgg
      181 gaaccgaggt tacggcacgg gttatcgagg cggggaagag gctcaagttt atcggtcgcg
      241 ccggtgccgg ggtggacaat attgataccg aggcggcgac ccggaagggc atcatagtcg
      301 cgaacgctcc tgaagggaac accctggcgg ccaccgagca cacgatggcg atgatgcttg
      361 ctctcgcgcg caatataccg caggctaacg cttcggtcaa gaagggcgag tggaagcgct
      421 cgaagttcat gggcgtcgag ctgaacgaga aggtcctggg tatcgtgggg ttcgggcgca
      481 taggccgtga ggtggcgaaa cgtgcgcagg cgatggagat gaagtgcatc gcttacgatc
      541 ctttcatcag cagcgagcgg gctgcaagcc ttggtgtgga gatggtgacg cttgaggacc
      601 tcttcaggag agcggacgtc atcaccgtcc ataccccgct tataaaggag acgcaccaca
      661 tgatcaacgc cgggacgatc gcgacgatga aggacggtgt tcggctgatc aactgcgccc
      721 gcgggggcat catcgatgag aaggctctcg cggacgggat tgcaagcgga aaggttgccg
      781 gtgctgcgct ggacgtcttc gagagcgagc cgccgacgga ttcgccggtg atcggtcttg
      841 agcaggtgat cgtcacgccg catcttgggg caagcacggt cgaggcgcag aagaatgtcg
      901 ccatctcgat cgcaaaccag tgtatcagcg tcctctcggg cgggtctgcg aagtacgtgg
      961 tgaacgctcc ggttgtgccg atggagcagc aggcgctgat agagccctac gcgaggcttg
     1021 cgcagaagat gggcaggctt ctcatccagc taatcgaggg caggcttgag gcgatcgatg
     1081 tcacctacgg cggggaggct gcaaacctgc cgaacacgaa gttcatcacg cgggtgatcc
     1141 tgaaggggat gcttgacccg atcctgcagg tgccggtaaa cctggtgaac gcggagtttg
     1201 tggcgaagga gcggggtatc cggttgagcg agaccaccac ggaggaggcg caggggttca
     1261 ggaacatcat caccataacg gcgaggactg acaggatgac ggagacggtt agcgggagcg
     1321 tctcgagccc gaaccgggcg cggatcgtga gcatcggggg ctacatgacc gatctgacgc
     1381 cgacggggca cgtggtcatc tcgcgccaca cggataagcc gggcgtcatc gggaaggttg
     1441 cgacgatcct tggccgggtg aacgtgaaca tcgcggggat gcaggtcggc cgccacaggc
     1501 cgggtgaaga ggcgctgatg gttctgaccg tcgattcgcc ggtgccgcct gaggcgatgg
     1561 aggagatcaa gcgggtcgat gggatccata cggcaaagta cgcggagatc tgagcgcgcc
     1621 gggatccccc tcggccctct cttctaaggg gcgattttga gccgggcgaa tggttttata
     1681 ccaaaagaac caaccttgtt gatcgtcagg ctgatgcggg ctcgtggtct aggtggttat
     1741 gacgtcgcct tgacatggcg gaggtcctga gttcgaatct cagcgagccc a
//
LOCUS       DAOSZX010000160         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_49698_length_1776_cov_15.679837, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000160 DAOSZX010000000
VERSION     DAOSZX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49698_length_1776_cov_15.679837"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="corA"
                     /locus_tag="PK196_08295"
     CDS             complement(<1..992)
                     /gene="corA"
                     /locus_tag="PK196_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HON41448.1"
                     /translation="MSPPEDASPGSGEPDLRPVTVTVIDYDAAHFSEWRCTRPEEIRL
                     PPSRRTVTWIIVDGVHDPAIVEAIGNALGIHPLTQEDIMNTRQRPKIEEYEDYLYVAV
                     RAVNLDGDEGFRSRQVSLLLGNGYIVSFQEQPDETFNRILKRLRAGGGRLRSVGPDYL
                     FYALLDAVVDGYFGVIEDFGERIETIEEEVMAEPDRETLQAIYAVKRSLIALRRSAWP
                     LRDVVAQLERGESPLISDALHIYFRDVYDHAIGVADTVETYRDMMSGILDIYLSSQSS
                     RMNEIMKVLTIIATIFIPLTFIAGIYGMNFEYMPELSHPFGYPAAIISMTAVAAA"
BASE COUNT          364 a          541 c          520 g          351 t
ORIGIN      
        1 gcggctgcaa cggccgtcat cgagataatg gctgccgggt atccaaacgg gtgagagagt
       61 tcgggcatgt actcaaagtt catgccgtag atcccggcga taaacgtgag cgggataaag
      121 attgtggcga tgatggtgag gaccttcatt atctcgttca tccggctgct ctggcttgag
      181 aggtagatat ccaggatccc tgacatcata tcccgataag tctccacggt atcggcgact
      241 cctatggcgt ggtcgtagac gtcgcggaag tagatgtgga gggcgtcgct gatcagcggt
      301 gactcccctc gttcaagttg tgcaacaacg tcacggagcg gccaggcaga acggcggagc
      361 gcgatcagtg aacgtttcac ggcgtatatc gcctggagag tctcgcggtc gggttccgcc
      421 atcacctcct cctcgatagt ctctatacgc tctccaaaat cctcgatcac accgaagtag
      481 ccatcgacga ccgcatccag aagggcgtag aagaggtaat caggaccaac gctccggagc
      541 cgccctccgc cagcacgcaa ccgcttgagg attcgattga aggtctcgtc gggctgctcc
      601 tggaaggata cgatatagcc attcccgaga agaaggctga cctgtctgct ccgaaaccct
      661 tcatcaccat cgaggttgac cgcacggaca gcgacataaa ggtaatcctc gtactcctcg
      721 atcttcggtc gctggcgggt gttcattatg tcttcctggg tgagggggtg tatcccgaga
      781 gcgtttccga tggcctcaac aatcgccggg tcgtgaacgc cgtcgacgat gatccaggtg
      841 accgtcctgc gagacggagg aagccggatc tcctcgggcc gagtgcatct ccactcggag
      901 aagtgtgctg catcgtagtc gatgacggtg accgtgacgg ggcgtagatc gggctcgccc
      961 gatcccgggc tcgcatcctc cggcgggctc attaatggta agatctatcc cggttatgat
     1021 gaatctgtcg gaaaaaatgc atgttgcgcg ggggggggcg aaggcatgcc cggaggtggg
     1081 ggtgttccca gcgagagagg ttttcagtga cacgcataga caggggcggg ggatgttccg
     1141 tcccccgccc cggcgagccc ctccccacgg gggcgattcc ccatagatac agtgtccggg
     1201 gaggagacca ctacttcgcg gtctcacctg acaccccctg agatccgttc ccgctccccg
     1261 tggacggcta tccggtcaat cgccacagac tgcccgcccc ctcggaaggg ggcgggagaa
     1321 ggccgcgaag cggccgggag atgggggtgt tctgcctcac acggaaaaga gcgcctggtt
     1381 gagacgcaga gacagcggcg gatcttccac cccagggcgt ggcgattctt catcgataca
     1441 ttgccatgga agagcatatc cagtgtcact ctgatcgctc ccccctctct cgttggaagg
     1501 cgttctgccg ctgtttttcg taaaattctt ctgaagaatc ccgggtccgc tccgccccgg
     1561 atacggttcc ctcccgcaga tcccccccac cacccagcca agccacgccc gggcgtcctc
     1621 acacctgaat ttcactttca tcccgcgcac ccaaactaca ttcatgcaga cgacaaaccc
     1681 ctaatgcaat ggactctctc gacgactggt tcccctaccg cgaataccgc ccctaccagc
     1741 gggagatgct cgaggtggcc gcatcaaccg cacgca
//
LOCUS       DAOSZX010000161         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_50686_length_1749_cov_21.240850, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000161 DAOSZX010000000
VERSION     DAOSZX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50686_length_1749_cov_21.240850"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1705)
                     /locus_tag="PK196_08300"
     CDS             complement(158..1705)
                     /locus_tag="PK196_08300"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HON41449.1"
                     /translation="MEPLTSEGIVEKIRKIEEGVGRLSPMQKVIIGTDGSVTGLLEMA
                     TGHPVSITTLVQDVVSADAGVAAELDIEPGEEINYRIVELRDSVTGEVLIYAVSVTPL
                     RRLAPEFRHDLMRADIPIGRILRHHRIESRREITDARVIQAGADLARSFKVNRCEPMV
                     SRRYRIIHQEEPLIVIEEIFPCTAFTDEIRVVVDAPSRIHITLIDMNGASGRVDGGVG
                     VTLDEPGCVLDARKATGIEVRGGSDEARHRVADAARATKEAFGLPGGAEIALHSTARQ
                     HAGLGSGTQIALATAAAICRLYERDVSVADLARVVGRGGTSGIGTAAFEMGGFILDGG
                     HRFGSPGGKEDFRPSSASRGIAPPPVLARHRFPEDWEILLVTPDVSAGAHGTEEVDLF
                     RTHCPVPLEEVREVCHEVLMRMIPGIVEHDLDLFGSAVNRTQQLGFKKVEVGLQHPVV
                     PALLEAMVQAGAAGAGLSSFGPTVYAIGDSGMADLARAAEEVLAGHGGSVTLTRARNC
                     GANMRAG"
BASE COUNT          331 a          603 c          495 g          320 t
ORIGIN      
        1 actggacacg gagaggttta tcaaaccagt gaggcattgg tttgatggcc tccgtatcac
       61 cctgtttcct ctcaacgcct caggaaagat cctgcgcatc cccgcgctcc agtccagacg
      121 cacgggtatg gcctgcagca ggagagaagg acgcctctta acctgcccgc atgttcgccc
      181 cgcagttcct ggccctggtc agggtcaccg aaccgccgtg gccggcgagc acctcttctg
      241 ccgcccgggc aagatcagcc ataccggaat cgcctatggc atagaccgtc ggcccgaaag
      301 aactcaaccc ggctccggcg gcaccggcct gaaccatcgc ctccagcagc gccggaacaa
      361 ccgggtgctg caggcccacc tccaccttct tgaatcccag ttgctgcgtc cggtttaccg
      421 ccgagccaaa gaggtcgaga tcatgctcaa cgatccccgg gatcatccgc atgagcacct
      481 cgtggcagac ctctcgaacc tcctcaagcg gcacagggca gtgggtgcgg aataggtcga
      541 cctcctctgt cccatgcgcc cctgcactga catccggcgt caccagcagg atctcccagt
      601 cctcggggaa acggtgccgg gcgagaactg gaggcggtgc gatccctctt gatgccgacg
      661 acgggcgaaa atcctctttc ccgcccgggg aaccgaacct gtgcccacca tcaagtatga
      721 aaccccccat ctcaaacgcc gccgtgccga tccctgatgt cccgccgcgg ccgacaaccc
      781 ttgcaaggtc agcgactgaa acatcccgct cgtacaggcg gcatatggcg gcggccgtgg
      841 caagcgcgat ctgtgtcccg ctgccgagcc cggcgtgctg ccttgcggtg gagtggaggg
      901 ctatctccgc accccccgga agaccaaacg cctccttcgt tgcccgggcc gcatcggcaa
      961 ccctgtgccg cgcctcatcg ctgccgccgc gaacctctat cccggtggcc ttgcgcgcgt
     1021 ccaggacaca cccgggctca tcgagagtga cgccaacccc gccatccacc ctgccggagg
     1081 ccccgttcat gtcgatcagg gtgatatgga tccgtgacgg tgcgtcgacc accacccgga
     1141 tctcgtccgt gaaagcggtg catgggaata tctcctcgat tactatcagc ggctcctcct
     1201 gatggatgat ccggtatctc cgcgagacca tcggctcgca gcggttgacc ttgaacgatc
     1261 tcgcaaggtc tgcaccggcc tggatgaccc tggcatcggt gatctcccga cgcgactcaa
     1321 tcctgtggtg acgaaggatc ctgcctatag ggatatccgc ccgcatcagg tcgtgccgga
     1381 actcgggggc gagacgccgg agcggggtga cagagaccgc gtatatgagc acttctccgg
     1441 ttacgctgtc tctcaactcc acaatgcggt agttgatctc ttctccgggc tcgatatcga
     1501 gctcagccgc caccccggca tcggcgctga caacgtcctg gacaagggtg gtgatgctga
     1561 ccgggtgacc ggtcgccatc tcaagcagcc cggtcaccga accgtcggtc ccgatgatga
     1621 ccttctgcat cggcgagagc ctgcccacac cctcctcaat cttgcggatc ttctcaacga
     1681 tcccttctga cgtgaggggt tccatatcag tgtaggcatt taatatggag agcacaaaaa
     1741 ggtggcggc
//
LOCUS       DAOSZX010000162         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_50690_length_1749_cov_12.667060, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000162 DAOSZX010000000
VERSION     DAOSZX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50690_length_1749_cov_12.667060"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1320)
                     /locus_tag="PK196_08305"
     CDS             complement(316..1320)
                     /locus_tag="PK196_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41450.1"
                     /translation="MDFEQIDDGIDSYEGVIVGVGGSDVLLTTGENALVGSVTFGNET
                     FWITPVESRVRAEGEASPLHVIYSSRDIENPEKWSLIDYGTLTPPEGYTPTEVPENLE
                     SSMRELQDQYATVNLLVVTDNQFYQDQDNWKTVAQDIVAEANRQFDRDDIRVALCVMA
                     YTDSRRVQLSNQPDILSKPVAAFERVYPNTDLDLWASDLALYVGGYDADGSAQGATYG
                     YYPDHHRHAWAQMVPDDTWYLGTTHGRRCVSIHELGHLFDTGHQDLDPNRTIPSYRRA
                     YQWYQPFSPPLKNTVTYSYFNELMSTTEFSSDDYHGDADHDNARRIRETKWTVANYHS
                     "
     gene            complement(1272..1670)
                     /locus_tag="PK196_08310"
     CDS             complement(1272..1670)
                     /locus_tag="PK196_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41451.1"
                     /translation="MKRKTGLRALSTLLAVMLVSVVVVSAVSAQDATGTPAVGGGEPA
                     LVVGDSMNVLIAGATSVSDIRASGIPEPEELNIPKGITRYDLVTFDHAALNKQVRGVL
                     PSRSTARSTRPNCTGWILSRSTTASTRTRG"
BASE COUNT          384 a          504 c          454 g          407 t
ORIGIN      
        1 tgaccagctc ctgcgtggga ccgtcgatcc ccgtgatcag ggcaacagcg gcggtatacg
       61 tgagcacgac cgtcgcgaca aggaggatga cgccgatgaa cgccggaacc ccgataacga
      121 gcagtcgttt gatgtttttc gcttccatgt gatttcctct gtgatttcac ccaatcccgc
      181 tgaaatagga tcgccctatt tccatacaga tggaccccca ttcatctcct ctgacgcgtc
      241 cgattcccgt accacccaaa gatctcaggt ggtgctttaa aaagatggat aggggtagac
      301 ttgcatctcc tcccatcagg agtggtaatt tgcaaccgtc cattttgtct cccggattct
      361 ccttgcgttg tcgtgatcgg catccccgtg gtagtcatcc gatgaaaatt cggtggtact
      421 catcaactca ttgaagtaac tatatgtcac ggtattcttg aggggaggag aaaacggttg
      481 ataccactgg taggccctac ggtaagacgg gatcgtccga ttaggatcca aatcctgatg
      541 accggtatca aagagatgcc ccagttcatg gatactcacg cacctgcggc cgtgtgtggt
      601 acccaggtac caggtgtcgt cagggaccat ctgggcccac gcatgccgat gatgatctgg
      661 gtagtagccg taagtagccc cctgtgcaga accatctgca tcgtaaccgc caacatacag
      721 tgcgagatcc gaggcccaga gatcaaggtc ggtgttcgga tagaccctct caaatgcagc
      781 aaccggcttg ctcaggatat cgggctgatt tgagagttga accctcctgg agtcagtata
      841 cgccattaca cagagcgcca ccctaatatc gtcccggtcg aactgccggt tcgcttcggc
      901 tacgatatcc tgagcgactg ttttccagtt gtcctgatcc tggtagaact ggttatcggt
      961 aaccaccagc agattgaccg tggcatactg gtcctggagt tctctcatgg aagattccag
     1021 attctcggga acttcggtcg gagtgtatcc ttccgggggc gtgagcgtcc cgtaatcgat
     1081 aagactccat ttttcaggat tttcaatatc ccgagagctg tagatgacat gcagaggcga
     1141 cgcttctccc tctgcgcgca ctcgcgactc gaccggggtg atccagaacg tctcgttacc
     1201 gaaggttacg ctcccgacaa gggcgttttc cccggtcgtc aggagaacat cgctcccgcc
     1261 aacgccgacg atcaccccct cgtacgagtc gatgccgtcg tcgatctgct caaaatccat
     1321 cctgtgcagt tcggcctggt actccttgct gtggacctgg aggggagtac accccgtacc
     1381 tgcttgttca gtgcagcatg atcgaacgtc acgaggtcat accgggtaat gcccttcggg
     1441 atgttcagtt cttccggctc cgggatcccg gatgcccgta tatcagatac cgatgtagca
     1501 cccgcgatga gtacattcat agagtcaccg acgaccaggg ccggctcgcc gccacctaca
     1561 gcaggagtcc ctgttgcatc ctgagcgctc accgcagaca caacaaccac gctcaccagc
     1621 attaccgcca ggagcgtgga gagtgcccgc aggccagttt ttcttttcat gtgtttcaac
     1681 tccgttttca tttaccggag gcaggagaag cacacgttta agtacgcaga acgcgtacac
     1741 ctcaatagc
//
LOCUS       DAOSZX010000163         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_52115_length_1712_cov_19.327097, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000163 DAOSZX010000000
VERSION     DAOSZX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52115_length_1712_cov_19.327097"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..893
                     /gene="cfbC"
                     /locus_tag="PK196_08315"
     CDS             75..893
                     /gene="cfbC"
                     /locus_tag="PK196_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HON41452.1"
                     /translation="MKQIALYGKGGIGKSTTAANLSAALAEEGLDILQIGCDPKHDST
                     RMLVHGTWIPTVLDLIRERGDANITVDDVVYRGFAGVRCVEAGGPEPGIGCAGRGIIA
                     TFQLLERLDALKGDVIVYDVLGDVVCGGFAMPMREGYAREIYLVTSGELMSIYAANNI
                     ARAIARLSRRARSRCSLGGVICNAKNIEGEAELVEEFARRINSRMIAYIPRSRIVQVA
                     ELQKQTVIEYAPESEQAGVYRDLARLVYGNDTTSIPTPMEMDEIESFALEFVQV"
     gene            853..>1712
                     /locus_tag="PK196_08320"
     CDS             853..>1712
                     /locus_tag="PK196_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HON41453.1"
                     /translation="MRSNPSPSSSSRFEGCTLTGALSVATEVRDAVCVIHGPAGCTHH
                     NFSLLHATSLSNDRPALPRLLSTSLVESDIIFGGEDALETALERALALSPASIFVLST
                     CIVETIGDDIAAVCAKDRGVPVIPIPTAGFLGGVFETGVKNALAAVASLAPPSSERTL
                     SANLIGEKNLEYGVDENAAEVARLLKRLGLPVNLRFVRGIRTHDLGRLGAAAVNILRE
                     PALGPLGEELKRRYGTPYIESFPTGLGGTCRFLEEVGRACGIDALAAVEDERAYQAAM
                     LERFDDIAGSR"
BASE COUNT          327 a          554 c          537 g          294 t
ORIGIN      
        1 gtatggcgat ccggttcatc tgccatgaac cgcacccgag acctaattat ccatgcgggc
       61 tcaatactga tccaatgaaa cagattgccc tctacgggaa agggggaatc gggaaatcca
      121 ccactgcagc aaatctctcg gcagcactgg cagaagaggg gctcgatatc ctgcagatcg
      181 gctgcgaccc caaacacgac agcacccgta tgctggtcca cggcacatgg atcccgacag
      241 tcctcgacct catccgggag cgcggagacg caaacatcac cgtcgacgac gtagtctaca
      301 ggggcttcgc gggcgtgcgc tgcgtggagg ccggcggccc ggagcccggc ataggctgcg
      361 ccggccgcgg gatcatagca acgttccagc tccttgagcg cctcgacgcc ctgaagggcg
      421 acgttatagt ctacgatgtc ctgggggatg tcgtatgcgg ggggtttgcg atgcctatgc
      481 gcgagggtta tgcccgggag atctacctgg tcacctccgg tgaactcatg tccatctacg
      541 ccgcaaacaa catagccaga gccatcgccc gtctatcccg ccgggcgcgg agcaggtgct
      601 ccctcggcgg cgtcatctgc aacgcaaaga acatcgaggg cgaggcggaa ctggtggagg
      661 agttcgcccg ccggatcaac tcccgcatga tcgcctacat cccgcggtcg aggatcgtgc
      721 aggttgcaga actgcagaaa cagaccgtca tcgagtatgc ccccgagtcc gagcaggccg
      781 gggtttaccg ggatctcgca cggctggtat acggtaacga tacaacgagc atccccacgc
      841 ccatggagat ggatgagatc gaatccttcg ccctcgagtt cgtccaggtt tgagggctgc
      901 accctgaccg gcgccctatc tgtggcaact gaggtgaggg acgccgtctg cgtcatccac
      961 ggcccggcgg gctgcaccca ccacaacttc tctctcctgc acgccacgag cctctcgaac
     1021 gaccgccctg ctctcccccg gctgctctcc accagcctgg tggagagcga tatcatcttc
     1081 gggggtgagg acgccctcga gaccgccctg gaacgtgcgc tcgccctctc gccggcgtcc
     1141 atcttcgtcc tctcgacctg catcgtcgag acgatagggg acgacatcgc tgcggtctgc
     1201 gcaaaggatc ggggcgtccc ggtgatcccg atccctaccg ccgggttcct tggcggcgta
     1261 tttgagaccg gggtaaaaaa cgccctggcc gccgtcgcat cgcttgcccc gccatcgagc
     1321 gagaggactc tctcggcaaa cctgatcggg gagaagaacc tggagtacgg cgtcgacgag
     1381 aacgccgccg aggtcgcccg gctcttaaag aggctcgggc tcccggtcaa cctgcggttc
     1441 gtgcggggga tcaggacgca tgacctcggc cggctcggtg ccgccgccgt gaacatcctc
     1501 cgggaacccg cgctcgggcc tctcggcgag gaactcaaac ggcggtatgg cacaccatac
     1561 atcgagtcgt tcccgaccgg actcgggggg acatgccggt tccttgagga ggtcggccgg
     1621 gcgtgcggca tcgatgctct ggccgccgtc gaggatgagc gggcatacca ggcggcaatg
     1681 cttgaaagat tcgatgatat agccgggagc cg
//
LOCUS       DAOSZX010000164         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_52401_length_1704_cov_23.522135, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000164 DAOSZX010000000
VERSION     DAOSZX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52401_length_1704_cov_23.522135"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..907
                     /locus_tag="PK196_08325"
     CDS             407..907
                     /locus_tag="PK196_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41454.1"
                     /translation="MRPAILAGCLLVMMLIAAPAGAFTADTLEVGVNEDGSANITFEY
                     TLSWLERIGVFFRIAEPEQELKAALEGAAGVPVTVTAAESDRAVFSALKFAKVTPGND
                     GVIYSTPEIDLTGAQKILDDYWFAPLVRADFSPDLTVVRFPDGYEERFNNQSTIPALS
                     HAVPKI"
     gene            complement(1088..>1704)
                     /locus_tag="PK196_08330"
     CDS             complement(1088..>1704)
                     /locus_tag="PK196_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HON41455.1"
                     /translation="WGVIGDKAGKATFVEVCSEKGANLLDAAAKAGAVVTEPANPKGI
                     EIRGKVENSMLKLGDKWRKRYFEDLGEGKERLQKIREETGRCIKCYACIENCPICYCV
                     ECSTKKSYLVEPGKVPPPFMFHLIRYAHIADSCINCGQCEEHCAMDIPNSLYMHALQV
                     EMEKMFGHTPGVNMELPVLALVEEHAERERLAATGSDQIFDIFK"
BASE COUNT          345 a          482 c          481 g          396 t
ORIGIN      
        1 ccctggagac agggtgggtg cctcttcagt tcaattttcc ccatagaatc gttctttgcg
       61 tgctgtgcac gaactttaag gggattgcaa ctcactgcct gccaggggcg gtggtttttt
      121 cctcgtcttt cgtctcaggt ggtctctgga gtcacatgac cgcacggttt ccgaggagtg
      181 gccagaggcg gaagtccccc gtagcaataa tctctggaat aaaagcaaag gctggccccc
      241 ttccattgcg atagatatat aatcacgggt atcgtatatc caggttagtc acgaaatggc
      301 cctttaagcc cagggaggga gaaatctaca tcaatgtgat gcaggatgcc ctttactttc
      361 aggttcgggt tagttcgcgt tgcaaaaggt atatagaggt ataaggatga gacccgcgat
      421 tctggctggc tgccttctgg tcatgatgct tatcgcggcc ccggcagggg ccttcaccgc
      481 agacactctc gaggtcggtg ttaacgagga tgggagtgcg aatatcacgt ttgagtatac
      541 cctttcctgg ctcgagcgga tcggcgtctt tttcaggatc gctgagccag agcaggaatt
      601 gaaggccgcg ctcgagggtg cggcgggcgt gcccgtaaca gtgaccgctg ctgagagcga
      661 tcgtgccgtc ttctcggctc tcaagttcgc aaaggtcaca cccggcaacg atggagtgat
      721 ctactccaca cccgagatcg atctcaccgg ggcgcagaag atccttgacg attactggtt
      781 tgcgccgctg gtcagggccg acttctctcc cgatctgacg gttgtcaggt tcccggacgg
      841 ctacgaggag aggtttaaca accagagcac catcccggca ctatcgcacg cggttcctaa
      901 aatataaata cccctttttc tgctcgagaa gcccgcgacc gaacccactt ctaagaccgg
      961 cctgcctgcg ctggattgta ctgaccaata atttcatatc cggcggggcg agaaccctcc
     1021 cccggggatg gagaccctgg aacgctgccg gggcttgagg actaaaaaaa gggtgggaag
     1081 gggatcctta cttgaatata tcaaagatct ggtcgcttcc ggttgcagca agccgttccc
     1141 gctcggcatg ttcttcaaca agcgcaagca cagggagttc catgttcacg ccgggggtgt
     1201 ggccgaacat cttctccatc tcgacctgca gagcgtgcat gtagagcgag ttcgggatgt
     1261 ccatagcgca gtgctcctca cactggccgc agttgatgca cgagtcagcg atgtgagcgt
     1321 atcggatcag gtggaacatg aacggcggcg ggaccttgcc gggctcgacc aggtaggact
     1381 tcttcgtgct gcactcgacg cagtagcaga tcgggcagtt ctcgatgcag gcgtagcact
     1441 tgatgcacct gcccgtctcc tccctgatct tctggaggcg ttccttgccc tcgcccagat
     1501 cctcgaagta gcgtttccgc cacttgtcgc cgagtttcag catcgagttc tcgacctttc
     1561 cgcggatctc gatacccttc gggtttgcag gctccgttac aaccgctccg gcctttgcag
     1621 ccgcatcgag aaggtttgcc cccttctcag agcagacctc aacgaaggtc gcctttccgg
     1681 ccttatcgcc tatgacaccc cagt
//
LOCUS       DAOSZX010000165         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_53049_length_1688_cov_14.649112, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000165 DAOSZX010000000
VERSION     DAOSZX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53049_length_1688_cov_14.649112"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..383
                     /locus_tag="PK196_08335"
     CDS             102..383
                     /locus_tag="PK196_08335"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HON41456.1"
                     /translation="MNIEEIKRKILPILKKYGVTRAGIFGSVVRGEAREESDIDILVE
                     IESRMSLLDFVGLKLELEEALGKPVDLGEYSEIKPIIKEQILEEEVPIL"
     gene            662..>1688
                     /locus_tag="PK196_08340"
     CDS             662..>1688
                     /locus_tag="PK196_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON41457.1"
                     /translation="MRDHALLHYDQTLFRDREIFEFGYTPERLHHRDAQTTELAWLVR
                     PALEGAAAGNAILRGPPGTGKTTTVRRLFAEIEDATNRIIPVFINCQQHRTQFSVFAR
                     IFEAVVGHAPPSAGKHVDEIVRQMAGRLEERDAALLVCLDDANYLHDARQLNDLLYRI
                     LRLYETRDVRRPGVFVVTSDRCLDINAAVDERVRSVFHPIEVNFPAYTKPEILEILGD
                     RVRQGLFPGVMPPSSLDLAAGITADEQDIRVGIDLIRRAVETVERTGRRRVTRKDVME
                     VSRSIIAPALQKRVEALPEAERGLLLRIAEMALEDADMTSGSVYEELRGYIAACPTVY
                     RARLKHLE"
BASE COUNT          405 a          497 c          474 g          312 t
ORIGIN      
        1 ggcgctggat aggttgtgat ctctctaaat ttgccatttc aggttacgag aaagaggctt
       61 ttgaatattc ataattcaaa ggatttgatg gaggatgaac gatgaatatt gaggagatta
      121 aaagaaagat attgcccata ttgaaaaaat atggggtaac aagagcaggt atttttggct
      181 ctgtggtaag aggggaagcg agggaagaga gcgacataga tatcttagtg gagattgaaa
      241 gcagaatgag tttacttgat tttgtaggat tgaagttgga actcgaagag gctcttggta
      301 aaccagttga tttgggagaa tactcagaaa tcaagccgat tattaaggaa caaatcctgg
      361 aggaagaggt gcctatccta tgaaaaggaa catgaaactt tacaggatat ctgggaaagt
      421 atgaagaata tgcccaggac ctcatcccgc ataagggttg cgttatattt caaactgccc
      481 cagttgcagg agcggtataa cgcaatcgat gaccgcatgc ccagcatcca gatccgcgtc
      541 ctgcaaaccc atccccgcaa agaaccgcgc cggtagaacg ctacaaaccc gcaccacccc
      601 ctttcacttt cacaccgcac ggtttgccat taataacaga atccagcaat agagattatc
      661 catgagagat cacgcactcc tccactacga ccagacactc tttcgggacc gggagatctt
      721 cgagtttgga tacacccccg agaggctcca ccaccgcgac gcccagacca cagaacttgc
      781 ctggctcgtc aggccggcgc tggagggcgc ggccgcgggg aacgccatcc tccgcggccc
      841 ccccggcacg gggaagacca ccaccgtccg ccgtctcttt gccgagatcg aggatgccac
      901 aaaccggatc atacccgtct tcatcaactg ccagcagcac cggacgcagt tctccgtctt
      961 tgcccggata ttcgaggcgg tcgtcggcca cgccccgccc tccgccggga aacacgtcga
     1021 cgagatcgtg cgccagatgg caggacgcct cgaggagcgg gacgccgcgc tgctcgtctg
     1081 cctggacgat gccaactacc tccacgacgc ccgccagtta aacgacctcc tctaccggat
     1141 cctccggctc tacgagaccc gggacgtcag gaggcccggc gtctttgtgg tcacaagcga
     1201 ccgctgcctg gatatcaacg ccgccgtaga cgaacgcgtc cgctcggtct tccacccgat
     1261 cgaggtcaac ttcccggcct acacaaagcc ggagatcctg gagatcctcg gcgaccgcgt
     1321 ccgccagggg ctcttccccg gcgtcatgcc gccctcaagc ctcgacctcg ccgccgggat
     1381 cacagccgat gagcaggaca taagggtcgg gatcgacctc atccggcggg cggtcgagac
     1441 cgttgagagg acgggccgca ggagggtcac ccggaaggac gttatggagg tctcccgctc
     1501 gatcatcgca ccggcgctcc agaagagggt cgaagccctc ccggaggccg aacgcggcct
     1561 cctcctccgg atcgcagaga tggcgcttga ggacgcggat atgacatcgg gctcggtcta
     1621 cgaggagttg cgcggctaca tcgccgcctg cccgaccgtc taccgggcaa ggctgaaaca
     1681 cctggagg
//
LOCUS       DAOSZX010000166         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_53569_length_1676_cov_21.267736, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000166 DAOSZX010000000
VERSION     DAOSZX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53569_length_1676_cov_21.267736"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK196_08345"
     CDS             complement(<1..216)
                     /locus_tag="PK196_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41458.1"
                     /translation="MHDPEIPIIAREHLEILQPHQGIDSLPDILMHELRIAELPDPTD
                     QGEKRDPTLRRNQRKKIVRRKSRRDPRQ"
     gene            286..>1676
                     /locus_tag="PK196_08350"
     CDS             286..>1676
                     /locus_tag="PK196_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41459.1"
                     /translation="MRQITYPGKGKKRRDTCGTIKKDENGREMWRACSGCGEAEPVSH
                     NCNSWECPECYTYRQGEWVKRTARRVFAYYECVKEHNAKVAREDRLPEYPPRHAGLNP
                     PMTYGGNVSRSPPVNRNSAIGWLDALLYSDDESPISWDLRRELCEDLLDGLNLLDDGV
                     CLSVEEMIEEAASVARPSYNGVLMPDDLQRAFREILIRESYKALEKSGLVGAAVIVHD
                     ERLTDGSREFAQCLADKLNRDLEAAFYEKAALRHEKNPGRYPEPSDLTYEGRRYNRYT
                     AAFTLDDYSDLLRFFPHIHIVCYGKFMDAREFQKVTGWNYENYSGNSKKDRALRSRGA
                     VEKTLRYLSSHMPILKGVQSVRYWGCLSYHRLKCVKGEKARLELPCRHCGKVMKEAVN
                     NGTAEAPDLEIDQGAPVTVAYVPRVYFLAGGPDPPGKGLGDLAHLVDALGDVPDRPRS
                     RIQMMREEFDKLRG"
BASE COUNT          340 a          404 c          527 g          405 t
ORIGIN      
        1 ctgccggggg tctcttctgc tttttctcct tacgatcttc ttcctctgat tcctgcggag
       61 ggtgggatct cttttttctc cctggtcggt cgggtctggg agctctgcga tcctcagctc
      121 atgcatgagg atgtccggca ggctgtctat gccctgatgc ggctggagga tctccaagtg
      181 ctccctggct atgatgggga tctccggatc atgcatagtg atcgttccct ctgtgcctgg
      241 caatactgcg caagtaaaaa aaagggtgga gttagggaat accagttgcg gcagatcacc
      301 tatccgggga agggtaaaaa acgccgggat acctgcggaa caataaagaa agacgagaac
      361 gggcgcgaaa tgtggcgggc gtgttctggg tgcggtgaag ctgaaccggt gtcccataac
      421 tgcaattctt gggagtgtcc ggagtgctac acgtaccggc aaggggagtg ggtgaagaga
      481 accgcccgga gagtgtttgc atactacgag tgcgtgaagg agcataatgc aaaggtggca
      541 cgagaagatc gccttcctga gtatcctcct cgtcatgctg gtctgaatcc cccgatgaca
      601 tatgggggta atgtttcccg gtcgccgccg gtgaaccgga acagtgcgat cggctggctt
      661 gatgctctcc tgtattctga tgacgagagt ccgatctcct gggatctccg gcgtgagctc
      721 tgcgaggatc tcctggacgg tctgaacttg ctcgatgatg gggtgtgcct ctcggtcgag
      781 gagatgatcg aggaggccgc tagcgttgct cggccttctt ataacggggt tctgatgcct
      841 gacgatctcc agagggcgtt tagggagatc cttatccggg agtcttacaa agccttggag
      901 aagtccggct tggtgggtgc tgcggttatc gttcacgatg agcggctgac agatggttct
      961 cgtgagtttg ctcagtgcct ggctgacaaa ctgaaccgtg acttagaggc cgcgttctat
     1021 gagaaggccg ccttgcggca cgagaagaat cctggtaggt atccggaacc ctcggatctc
     1081 acatatgaag gccggcggta taaccgctat acagctgcgt tcaccctgga cgactattcc
     1141 gatctgctgc ggttcttccc tcatatccat atcgtttgtt acggtaaatt catggatgcg
     1201 cgggaatttc agaaagtaac cggctggaac tatgagaact attctggcaa ttcaaagaag
     1261 gatcgcgctc tccggtcgcg gggagcggtc gagaaaactc tccggtatct gagttcccat
     1321 atgccgattc tcaaaggggt gcagtccgtc cgatattggg gatgcctatc ctatcatcgg
     1381 ctgaaatgcg tgaagggaga gaaggcacgg ttagaacttc cgtgccggca ctgtgggaaa
     1441 gtgatgaagg aggccgttaa taacggtact gctgaagctc cggatctcga gatcgatcag
     1501 ggagctccgg ttacggttgc ctatgtccct cgggtgtact tcttggcggg gggtcctgat
     1561 cctcctggga aaggcctcgg tgatcttgct cacctggtgg atgccctggg cgatgttccg
     1621 gatcggcctc ggtcgcggat acagatgatg cgagaagagt tcgataagct ccgggg
//
LOCUS       DAOSZX010000167         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_53740_length_1672_cov_23.641929, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000167 DAOSZX010000000
VERSION     DAOSZX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53740_length_1672_cov_23.641929"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="lysS"
                     /locus_tag="PK196_08355"
     CDS             complement(<1..1146)
                     /gene="lysS"
                     /locus_tag="PK196_08355"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HON41460.1"
                     /translation="MSDREHLCFEDTKIEKIQRLRERGVTIYPYTFDRRDSIAGIRER
                     FSEVGHEKSEEEVRTAGRVYVIRQHGKTIFADIGDAGGRIQIYIRKNDLGEEEFDLFR
                     QYIDTGDIIGVVGHPFRTKMGELTIWVDRFELLAKSLCPLPEKFHGLKNVEARYRQRY
                     LDLIMNEETCRVFRTRSRIISLLRQYLFERDYLEFETPTLQPIYGGANARPFTTYHNY
                     LEQKFYLRIAPELYLKRLVVGGFDKVFEIAKNFRNEDIDTNHNPEFTMVEIYEAYRDY
                     NDMMNLTEEIITGLVEQLHGRPACTLAGHDLDFSRPWRRISMEDAVREYAGVDVYAHS
                     VDELRSFARDHGVEGYEAAATRGEFLALLFEHFAEEKLVQPTFVHDFP"
     gene            complement(1412..1648)
                     /locus_tag="PK196_08360"
     CDS             complement(1412..1648)
                     /locus_tag="PK196_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41461.1"
                     /translation="MKQIPFLLFALLLLASGAGCIVSWDTDTGKRHPRPVLHCERGNV
                     SIPVNISEIPVKRFDVNATEVIEILLTNPRRDPA"
BASE COUNT          314 a          460 c          509 g          389 t
ORIGIN      
        1 ggggaagtcg tggacaaaag tcggctggac aagtttttcc tcggcgaaat gctcaaagag
       61 gagtgcaagg aactcgccac gggttgcggc cgcctcgtag ccttcgactc cgtgatcccg
      121 ggcgaaactg cggagttcgt cgacgctgtg ggcgtagacg tcgacccccg cgtattcgcg
      181 gaccgcgtcc tccatcgaga tgcggcgcca tggcctggag aagtccaggt cgtgccctgc
      241 aagggtgcat gcaggtctgc catgcaactg ctcaacgaga ccggtgatga tctcctctgt
      301 gaggttcatc atatcgttgt aatcacggta agcctcgtag atctccacca ttgtgaactc
      361 cgggttgtgg ttggtgtcga tatcctcgtt cctgaagttc ttggcgatct caaaaacctt
      421 atcaaacccg ccgacgacca ggcgcttgag gtagagttcc ggtgcgatac ggaggtagaa
      481 cttctgctcg aggtagttgt ggtaggtggt gaacggtcgg gcgttggcac cgccgtagat
      541 cggctgcagg gtgggggtct caaactccag gtaatcgcgc tcgaagaggt actggcgcag
      601 gagtgatatg atcctgctgc gtgtccggaa taccctgcag gtctcctcgt tcatgatcag
      661 gtcgaggtaa cgctgccggt aacgggcctc gacgttcttg agcccgtgga acttctccgg
      721 cagagggcag agtgatttgg cgaggagttc gaaacggtcc acccagatcg taagttcgcc
      781 catcttcgtc cggaagggat ggccgacgac gcctatgata tcccctgtat cgatatactg
      841 gcggaagagg tcgaactctt cttccccaag gtcgttcttc cggatgtaga tctgtatcct
      901 gccgccagcg tctcctatat ctgcaaaaat cgtttttcca tgctggcgta tgacgtagac
      961 gcgaccggcg gttctgacct cttcttcgct tttttcgtgt ccgacttcgc tgaatcgctc
     1021 cctgatcccc gcgatgctgt ctctgcggtc gaaggtgtag gggtagatcg ttaccccgcg
     1081 ttcgcgcagg cgctgtatct tctcgatctt tgtatcctca aagcagaggt gttctctatc
     1141 gctcataatg taattcgctc cattcgtccc gcagccctga acctggctgg aaatcctggt
     1201 tggacgccgt tccggcgaga ccgggccgca cgtttgccgg gaaattctat atactgttca
     1261 cccgccgggt attaattttg aacggcgggc cgtaggccca ggccggggca ggagagaccg
     1321 cgcgccctgg agcagcaggg gctcaggtcc accagaccct ccgcggcttc gagccattcc
     1381 cgtccggatc gaaatgttct gccagccgcc ctcaagcagg atcccggcgc gggttggtga
     1441 ggaggatctc gatcacttct gttgcgttga catcaaacct tttcaccggg atctcggaga
     1501 tgttgacggg gatcgagacg tttccccgct cgcagtggag gaccggccgg gggtgacgtt
     1561 ttccggtgtc cgtgtcccag gagacgatgc accccgcccc ggaggccagg aggagcagtg
     1621 caaaaaggag aaagggaatt tgtttcatag ttcatctgtt gtaggtatgg ag
//
LOCUS       DAOSZX010000168         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_54501_length_1655_cov_20.403125, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000168 DAOSZX010000000
VERSION     DAOSZX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54501_length_1655_cov_20.403125"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1655)
                     /gene="carB"
                     /locus_tag="PK196_08365"
     CDS             complement(<1..>1655)
                     /gene="carB"
                     /locus_tag="PK196_08365"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HON41462.1"
                     /translation="SGVLTYKMVDTCAAEFPATTPYFYSTWEDGCELTRNDTRKVLIL
                     GSGPIRIGQGIEFDYCTVHAVMALREEEGIEVHIINNNPETVSTDADTSDRLFFEPMH
                     LEDVMNILKKDDYYGVMVQFGGQNSVNLAVPLEAEIRRLGLETKILGTSPDAMDVAED
                     RDRFSRLLKRLGIPSPANSSAYSEKEALEIARKIGYPVLVRPSYVLGGRAMELVHDDA
                     ELEGYIKEAVRVSRKHPVLIDSFLQGAVEIDVDAVCDGTDVLIGGIMEHIEWAGVHSG
                     DSACVIPPQSLSPSVIERVREYTKKIALGLGVIGLINIQYAVQNDVVYVLEANPRASR
                     TVPFVAKATGIPLAKIAAKVMVGRKLADMGVAEREIRHVAVKEVLLPFNKLPGVDTVL
                     GPEMKSTGEVMGIDYDFGRAYYKASTAAENTLPTEGNVFISVRDEQKEELLPIARKLR
                     ELGLTLYGTSGTVEFLAQNGIEANLVRKVQEGSPNVIDAMRSGSIRLIINTPADKASR
                     QDHIQIMRAAVDYAIPYITTLQAARAAAMAIEAIKHAEITLEPL"
BASE COUNT          307 a          531 c          461 g          356 t
ORIGIN      
        1 agcggctcga gcgtgatctc tgcatgcttt atggcctcga tcgccatcgc ggccgccctg
       61 gcagcctgga gcgtcgtgat gtaggggata gcgtaatcca cagcagccct catgatctgg
      121 atgtggtcct gccgggaagc cttgtccgcc ggggtgttga tgatcagccg gatgctcccc
      181 gacctcatag cgtcgatgac gtttggcgac ccctcctgca ccttccgcac aaggttcgcc
      241 tcgatgccgt tctgggccag gaattcaacc gtcccgctcg ttccgtagag ggtgaggccg
      301 agttccctga gttttcgtgc gatcggcagg agttcctcct tctgctcgtc cctcaccgat
      361 ataaagacgt ttccctccgt cggcagggtg ttctccgcgg cggtagatgc tttgtaatag
      421 gcgcggccga aatcgtagtc gatccccatc acctcacccg tactcttcat ctccgggccg
      481 agcacggtgt caacgccggg gagtttgttg aagggcagga gaacctcctt caccgcaacg
      541 tgccggatct cgcgttcagc aacacccatg tcggcaagtt tcctgccgac catcaccttt
      601 gccgctatct ttgcaagcgg tatccccgtt gcctttgcaa cgaacggcac cgtccggctc
      661 gcgcgggggt tagcctccag cacgtagacc acgtcgttct ggacggcgta ctggatgttt
      721 ataagcccta taaccccgag acccagggca atcttttttg tgtattcgcg cacccgctcg
      781 atcaccgatg gcgagagcga ctgcggcggg atgacacagg ccgaatcccc cgaatggact
      841 cccgcccact cgatgtgctc catgataccg ccgatcagaa catccgtccc gtcgcagaca
      901 gcatccacat cgatctcgac agcgccctgg aggaacgagt cgatcagcac cgggtgcttc
      961 cggctcaccc ggacggcctc cttgatgtaa ccctcaagtt cggcatcgtc atggacgagt
     1021 tccatcgccc gtcccccgag gacgtaagac ggtctgacaa gcaccgggta gccgatcttc
     1081 ctggcaatct caagcgcctc cttctccgag taggcggaac tgttcgccgg gctcgggatc
     1141 ccgagacgct tcaggaggcg gctgaaccgg tcacggtcct ccgccacgtc catggcgtcg
     1201 ggagatgtcc ccaagatctt tgtctcaaga ccaagacgcc tgatctccgc ctcgagcggc
     1261 acagcaaggt tcacagagtt ctggccgcca aactggacca tcacaccgta atagtcgtct
     1321 ttcttgagga tgttcatgac gtcctcaagg tgcatcggct caaagaagag cctgtcagat
     1381 gtgtcggcat cggttgagac cgtctcaggg ttgttgttga tgatatgaac ctcgatcccc
     1441 tcttcctcgc gcagcgccat aaccgcgtgg acggtgcagt agtcaaactc gatcccctgc
     1501 ccgatcctga tcggcccgga accaaggatc agcaccttcc ttgtgtcgtt ccgggtgagt
     1561 tcgcagccgt cctcccaggt ggagtagaaa tacggtgtgg tcgccgggaa ctcggcggcg
     1621 catgtatcaa ccatcttgta ggtcagcacc ccgga
//
LOCUS       DAOSZX010000169         1589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_57471_length_1589_cov_23.820730, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000169 DAOSZX010000000
VERSION     DAOSZX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57471_length_1589_cov_23.820730"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..>1589
                     /locus_tag="PK196_08370"
     CDS             7..>1589
                     /locus_tag="PK196_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HON41463.1"
                     /translation="MKSTIRIKTINGKEYWYEDIPYYDKEKKQIRHRSKYLGKNIDGK
                     PVRVRSEPMIPASTRVADAPVGAYTHGNLLPLQKITQELSIRSTLEEFLNPEETDIIL
                     ALVYNRVLRPLALQNVQTWFEGSSLSLQHPHLPLASQRLSEILAALGKSNVPSLFVDR
                     FASQTKPGATLLYDITSLSSSSKLLELLEYGYNRDSDGLPQVNVSLVMDKERGIPIRY
                     DLYPGSIADIGTLANTLHWLKTMEIDQFRLILDRGFFSQTALAELLDKEIPFIIPPTL
                     HLTSVKELMSTSQKKVGRAEFLHKYEKEAIFAMPVTLSHLYELEEEPRVLTVKGYCYY
                     DPQREQSERSAFYQHLHDVVEKVQHAQPRAWKRPDVVVGEIAKHFARYLSWKFVDGRF
                     EVSIKQKAVTQRVNRMGRFILLYSGEMDWLTCLTLYRQRDAIEKCYEVMKNDLKALPL
                     HLRTEETLRGHLFITFLALVLRMRLQMMMKETGLIKQYSVQKLLLELEKIKMVELSSG
                     EKMVSEVSKKNRMILEALG"
BASE COUNT          393 a          426 c          427 g          343 t
ORIGIN      
        1 gcagcaatga agtccaccat ccgcatcaag acgatcaatg gcaaggaata ctggtatgag
       61 gacatcccct actacgacaa agagaaaaag cagatccggc atcggtccaa gtacctcgga
      121 aaaaatatcg acgggaaacc ggtccgggtc cggagtgagc cgatgatccc tgccagtacg
      181 agggtcgccg acgccccggt cggtgcctac acccatggaa accttctgcc gctccagaag
      241 atcacccagg aactgtcgat ccggtcaacg cttgaggagt tcctgaaccc ggaggaaacc
      301 gacatcatat tggctctcgt ctataaccgt gttctccggc cacttgcgct ccagaacgtg
      361 caaacctggt ttgaagggtc atctctttcc ctccagcatc ctcacctccc gcttgcgagt
      421 cagcgtctca gcgagatact tgcagcgctc ggaaagagca atgttccctc cctgtttgtg
      481 gaccgatttg cttcccagac caaacccggt gcaacgcttc tctacgacat taccagtctc
      541 tccagttcct ccaaactcct ggaactcctg gaatacgggt ataaccgcga cagtgatggt
      601 cttccgcagg tcaacgtctc ccttgtcatg gataaggagc gtgggatccc gattcggtac
      661 gatctctatc ccgggagcat cgccgatatc ggaacgttag caaacacgct gcactggctg
      721 aaaaccatgg aaatcgacca gttcaggctg atcctcgacc gggggttctt ttcgcagacg
      781 gctcttgccg aactgctgga taaggagatc cccttcatca ttcccccgac gctgcacctc
      841 acctcggtga aagaactgat gagtacttcg caaaagaagg ttggtagagc ggagtttctg
      901 cacaaatacg aaaaagaagc gatctttgcg atgccggtca ctctttccca cctttacgaa
      961 ctcgaagagg agccccgggt gttgaccgtc aaggggtact gctactacga tcctcaacgc
     1021 gagcagagcg agcggagtgc gttctaccag cacctgcatg atgtcgttga gaaggtgcag
     1081 catgcccagc caagggcatg gaaacggccc gatgtggtgg taggagagat tgccaagcat
     1141 tttgcccgct atttatcctg gaaatttgtg gatgggcgat ttgaggtctc gatcaagcag
     1201 aaggcagtaa cccagcgggt taaccggatg ggacgattca ttctcttgta ttcaggggag
     1261 atggactggc tcacgtgcct taccctgtat cggcaacgag acgccatcga gaaatgttac
     1321 gaggtgatga agaacgacct caaggccttg ccgttgcacc tgaggactga ggagaccctg
     1381 cggggacatt tgttcatcac gttcctggca ctggtcctgc gaatgaggtt gcagatgatg
     1441 atgaaagaga cggggctcat caagcagtac tcggtccaaa aactgctcct cgaactcgaa
     1501 aagatcaaga tggttgagct atcttccggg gagaagatgg tttctgaggt cagtaagaag
     1561 aacaggatga ttttagaggc actcggatt
//
LOCUS       DAOSZX010000170         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_61075_length_1519_cov_21.372951, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000170 DAOSZX010000000
VERSION     DAOSZX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61075_length_1519_cov_21.372951"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..518
                     /locus_tag="PK196_08375"
     CDS             60..518
                     /locus_tag="PK196_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HON41464.1"
                     /translation="MLLEGMTLGWIMIVLGAVLLLIEVCQPGFFLAVPATVLFILGVL
                     LLLGVDILTSPWGLVIGIIAAILAAIVTIWLYGRITPAEKPPTTLSRDSLVGLEGVVV
                     REVDDETLAGKVQIGSMEWSARSESGRIPAGRRVVVLRAEGVHIVVKEVA"
     gene            520..>1519
                     /locus_tag="PK196_08380"
     CDS             520..>1519
                     /locus_tag="PK196_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HON41465.1"
                     /translation="MALIESAPWTGLITIFLIIVIVIIFASGVVIVQPYEQGLQIRLG
                     QYIGRLNPGFRWVVPLITTVRKLDLRTEVMDVPRQEVITKDNSPTNVDAIVYVRVIDP
                     EKAFFEVMNYRSATVALAQTSLRGIIGDMELDEILYNRDIINARLRDILDRETDAWGV
                     KVERVEIKEVDPVGPVKQAMTEQTAAERERRAAILRADGEKRAAILRAEGNRQSIILE
                     AEGERQSKILRAEGERLSRILQAQGEAQGLRILSVGARPLDKRAITVLSLDALKQMAD
                     GQATKIIFPFELSSVVKQAADYLGATAEAPDVPEGAEVPPEDILGEIPGRDVVQTAE"
BASE COUNT          282 a          426 c          515 g          296 t
ORIGIN      
        1 tccacgtccc gtaaggtttt ttcagatcct ttttatcccc tgcacaccat ctatcatcca
       61 tgcttcttga ggggatgaca ctgggctgga tcatgatcgt gctcggggcg gtgctgctcc
      121 tgattgaggt ctgccagccg gggttcttcc tcgccgtccc tgcaactgtc ctcttcatcc
      181 tgggggttct tctcctccta ggggtggata tcctgacctc tccctggggg ctggtcatcg
      241 ggataattgc ggcgatcctt gcggctattg tcaccatctg gctttatggc cgtatcaccc
      301 cggccgagaa acctccgaca acgctctccc gcgactcgct tgtcggtctt gaaggggtgg
      361 tggtccggga ggtcgatgac gagacgctcg ccgggaaggt gcagatagga agcatggagt
      421 ggagtgcgcg ttcagaatcc ggtcgtatcc cggcggggcg gagggttgtt gttctccggg
      481 ccgagggcgt gcatatcgtt gtgaaggagg tggcctgagg tggcattgat agagagcgcc
      541 ccctggacgg ggctgatcac catattcctg atcatcgtca tcgtcatcat ttttgccagc
      601 ggtgtggtga tagtgcagcc ttacgagcag ggtctccaga ttcgtctggg gcagtacata
      661 gggagactga acccggggtt caggtgggtt gtgccgctga tcacgacggt caggaaactc
      721 gacctccgaa cagaggtgat ggacgtcccg cgccaggagg tgatcacgaa ggacaactcc
      781 ccgacaaacg tggacgcgat cgtctatgtc cgggtgatcg accctgaaaa ggccttcttc
      841 gaggtgatga actaccgctc tgcgacggtg gcgctggcgc agacgagtct gcgcgggatc
      901 ataggcgaca tggagctcga cgagatcctc tacaaccgtg atatcatcaa cgcacgtctc
      961 cgggacatcc ttgaccggga gacggatgcc tggggtgtca aggtcgagcg ggtggagatc
     1021 aaggaggtcg acccggtcgg accggtcaag caggcgatga ccgagcagac cgcggccgag
     1081 cgtgagcgcc gggcggcgat cctccgtgct gacggagaga agcgggcggc gatccttcgg
     1141 gcggaaggca atcggcagag catcatcctc gaggccgagg gcgagcggca gagcaagatc
     1201 ctgcgggccg agggcgaacg cctctccagg atcctgcagg cacagggcga ggcgcagggg
     1261 ctgcgcatcc tatcggtagg cgccaggccg cttgacaaac gggcgatcac ggtgctctcg
     1321 ctcgatgccc tcaaacagat ggccgacggt caggcgacaa agataatctt cccattcgag
     1381 ttgtcgagcg tcgtcaaaca ggccgcggat taccttggcg caacggccga ggccccggat
     1441 gtccccgaag gcgcggaggt gccgccggag gatatcctcg gggagatccc gggccgggac
     1501 gtggttcaga ccgcggagg
//
LOCUS       DAOSZX010000171         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_62138_length_1500_cov_12.574394, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000171 DAOSZX010000000
VERSION     DAOSZX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62138_length_1500_cov_12.574394"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..140
                     /locus_tag="PK196_08385"
     CDS             15..140
                     /locus_tag="PK196_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41466.1"
                     /translation="MHLYCRLNLDEVYGVLQNDLPVVNQYRDLVRAMLRDGLLPE"
     gene            complement(880..1161)
                     /locus_tag="PK196_08390"
     CDS             complement(880..1161)
                     /locus_tag="PK196_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HON41467.1"
                     /translation="MFAILIERRAREFLKGLPPKTRRIVVEKIQELAENPFPGGDKEK
                     LEYPHPPAVYRLHISRSFTVFYIIEPEENVVKIEKIMTIEKAHKEYSRR"
     gene            complement(1183..1413)
                     /locus_tag="PK196_08395"
     CDS             complement(1183..1413)
                     /locus_tag="PK196_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41468.1"
                     /translation="MSSATRDTGSQIKRIPVKEPTWRDLHGLKEAGESYDDLLSRMIR
                     RERDYRDWKMIVEIEESGEFVAFDPDEILQDD"
BASE COUNT          293 a          449 c          401 g          357 t
ORIGIN      
        1 ttcggaatgt tctggtgcac ctctactgcc ggctgaacct ggacgaggtc tacggcgtgc
       61 tccagaacga cctcccggtc gtgaaccagt accgcgacct ggtccgggcg atgctccggg
      121 acggcctcct cccggagtga accgggggat gcaccccggc ttttttgacc tcaggcagtg
      181 agacaacgaa tgtatagacg ttcccgtctg caacccccta gcccgtgaag atttttccga
      241 ccaggtcgat aaaggcggtg gcaagttcca gagctttctt ccccttcctc cgggtggtga
      301 tataatagac gccaggcgtc cctgcccgcg agacggtatt ggaaggtgat attgagtgag
      361 gaactctccg cgacgaaacg ggccgagcgc tcgttcctca ccccgagagg tacagaaacg
      421 ccttgatggc tttctcttcc gcctgccggg caggaaagca ggttaggtta taacctctcg
      481 tcgccagcaa aggcatactg cgcatcatca gggtcgcgtt ctgcttgttt taaccattct
      541 gttcccttct tgttccggat tccgcatcgt agaccacaac accatcctgt aacgcattct
      601 taagaaggaa tcaactttga caaatcatcg cgcatcatca cgcatttatc gcgcatcgcc
      661 tccctgttcc cctccctcgc ccggagcgag aatatagtgg gtgtcgcgcc gcgatcggcc
      721 tctccgttca gcgatgcgcc tcctgacagg gtcgtgtgaa ctgcaccgca cctgttagcc
      781 ggagagttcc cgctctgccc cccgcaagat aaactacgga ctgcagcccc gaatcccccg
      841 gtgaaaggaa gggtgaaaca cctgtgtccg gggaggaggc tacctgcgcg aatactcctt
      901 atgggctttc tcgatcgtca tgatcttctc gatcttaacg acgttctcct caggctcgat
      961 gatgtaaaag acagtgaagg atctgctgat atggaggcgg tagacggcag ggggatgggg
     1021 atactcaagt ttttccttgt ctcctcccgg aaacgggttc tccgcgagtt cctggatctt
     1081 ttcaacgacg atacgccggg ttttgggggg gagccccttt aagaactccc gtgcccttct
     1141 ctcgatcaga atggcaaaca cgctttcccg ctttccggta cctcagtcgt cctgcaggat
     1201 ctcgtccggg tcgaaggcta cgaactcgcc gctctcctcg atctcgacga tcatcttcca
     1261 gtcgcgatag tcccgctctc gccggatcat ccgggacagc agatcgtcgt aactctcccc
     1321 tgcctctttg agaccgtgca ggtccctcca ggtgggttct ttcacaggaa tgcgcttgat
     1381 ctggctgccg gtatcgcgtg tggcggaaga catgcatatc tgtagatggg gatggtattt
     1441 atcgatttgc atatttgcca caatgaataa aagtgccact cgtgctgtgc tcctgccccg
//
LOCUS       DAOSZX010000172         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_62481_length_1494_cov_18.193885, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000172 DAOSZX010000000
VERSION     DAOSZX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62481_length_1494_cov_18.193885"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1360
                     /locus_tag="PK196_08400"
     CDS             170..1360
                     /locus_tag="PK196_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HON41469.1"
                     /translation="MAAREIVPGVFAVGAIDWDRRLFDELIPLPDGTTYNSYLVRGSE
                     KTVLIDTVDPTKTSELITNLESLGVDKIDYLVASHAEQDHSGSIPAVLERFAGVKVVT
                     NQKCRDLLIALLHVPEEKFIVIDDGDTLDLGGKTLEFIIAPWVHWPETMLTYLAEDRV
                     LFPCDLFGSHYATSSLYVPDECAVYEAAKRYYAEIMMPFRASIRGHLEKLATREIEII
                     APSHGPVHRRPAFIIDAYRDWTGDAVKNIVVLPYVSMHGSTQAMVDHLLDALINRGIP
                     AQPFNLTKTDTGDLAKALVDAATIVIGAPTVIFGPHPQVVYAAYLANLLRPKTRYATV
                     IGSYGWGGKTVDTIKGMLDRLKVEVLEPVYIRGYPTAEDFAALDRLADEIERKHREAG
                     IIST"
BASE COUNT          342 a          435 c          431 g          286 t
ORIGIN      
        1 gttagagcga aactgttatc agcattcgcg ccggagaaag gtttgccgac cggtggggca
       61 cgaacgccct cgccggcacg gtcaagaacc aatggagctg aaaaggcatg tcgaatacgc
      121 tgataaaccg gataccctcc gggttagagg atacggaggt gatgggataa tggccgcgcg
      181 tgagattgtg ccgggagtct ttgccgtagg cgccatcgac tgggaccgcc gactcttcga
      241 cgaacttatc ccgctccctg atggcaccac ctacaacagt tacctggtgc ggggcagcga
      301 gaagacggtg ctgatcgata ccgtcgaccc tacaaagacc agtgaactca tcacgaacct
      361 ggagagtttg ggggtcgaca agatcgatta cctcgtcgcc agccacgccg aacaggacca
      421 ctcggggtcc atcccggcag tgcttgagag gttcgctggg gtgaaggttg tgacaaacca
      481 gaagtgccgc gacctcctga tcgctcttct gcatgtcccg gaggagaaat tcatcgtaat
      541 cgacgacggc gacaccctcg acctgggcgg taagacactg gagttcatca ttgccccatg
      601 ggtccactgg ccggagacga tgctgaccta tctcgcggag gatcgcgtcc tattcccctg
      661 tgacctcttc ggttcgcact acgcgacgag ttcgctctac gtccctgacg agtgcgcggt
      721 ctacgaggct gcaaaacgct attacgccga gatcatgatg ccgttccgcg cgagcatcag
      781 ggggcacctt gagaaactcg cgacgcgcga gatagagatc attgcaccga gccacggccc
      841 cgttcacaga agaccggcgt tcataatcga tgcttacagg gactggacgg gcgatgccgt
      901 gaagaatatc gtggttctcc cctacgtctc catgcacggg agcacgcagg ccatggtcga
      961 ccatcttctt gacgccctga taaaccgggg tatcccggct cagccgttca acctcacaaa
     1021 gaccgataca ggcgatctcg caaaagcact cgtagacgcc gcaacgatcg tgataggggc
     1081 gccgacggtc atcttcggtc cacacccgca ggtggtgtat gcggcatacc ttgcaaacct
     1141 gctccgcccg aagacgcgct acgccacggt gatcggctcc tatggctggg gtggaaagac
     1201 ggtcgatacc ataaaaggga tgcttgaccg actcaaggtt gaggttctgg aaccggtcta
     1261 tatcaggggt tacccgaccg cggaggattt tgccgcgctc gaccggctcg ccgatgagat
     1321 cgagaggaaa cacagggaag cagggataat ctccacctga acctcaccat ttttctgctg
     1381 gacgctcctg agcccatcaa atcagcggtc ttgaatattc aatcgagagc cagcccgctc
     1441 tcaatcgaac cgcttgagta gacctgtaaa gagcatgatc accgggatga cctc
//
LOCUS       DAOSZX010000173         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_64006_length_1468_cov_14.237084, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000173 DAOSZX010000000
VERSION     DAOSZX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64006_length_1468_cov_14.237084"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK196_08405"
     CDS             <1..672
                     /locus_tag="PK196_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HON41470.1"
                     /translation="YDIAEGLAARGFIAIRQGNPRVYVPADPAVVIRRLSGDLDAAAT
                     AALQGLEALSLDARSKNSPIRYVQGEWGIGRHLESLLAGLSRDLAVVCLDYREIGEFA
                     RQIADASRDHPVSILLPNGRRGIDRPLGNASLYIPRPFCPFFQEKIFERIYCGLIPVD
                     GSAFQLDYIFIADDRACMIVYRQDGLRNAVVVTLPFITCVQREFVNRMIANADCIGGQ
                     KPSGG"
     gene            complement(681..1376)
                     /locus_tag="PK196_08410"
     CDS             complement(681..1376)
                     /locus_tag="PK196_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON41471.1"
                     /translation="MENQPRGAILQRDGATYAIVPRTPAGIVSPEDLENIIKVVRRYS
                     IPIIKMTSGQRMVLVGIKEEDVEKIWNELGMTVGQATAPCVHYVQACPGTETCRYGKQ
                     DSLGLGLKIEEIYQDLNMPAKVKIGVSGCPRSCGESYLRDIGLLGTAKGWRVIFGGNA
                     GARPRIGDLVAKDLTTDEALDLVRRLLEYYRSNAERGERTARFVERVGFDAIRKDVLA
                     LTPYIPLESTRPD"
BASE COUNT          229 a          474 c          452 g          313 t
ORIGIN      
        1 tacgacatcg ccgaagggct tgccgccagg ggtttcatcg cgatccggca ggggaacccc
       61 cgcgtatacg ttccggccga cccggccgtt gtcatccgcc gcctctcggg cgatctcgat
      121 gcggcggcaa cggccgcgct gcaggggctc gaggccctct cgcttgacgc ccggtcgaag
      181 aactctccta tccggtacgt tcagggggag tgggggatcg gccgtcacct ggagtcgctc
      241 cttgccggcc tctcccgtga ccttgcggtc gtctgcctgg actaccgtga gatcggggag
      301 ttcgcccgcc agatcgccga tgcctcgcgc gaccacccgg tgagcatcct cctcccgaac
      361 gggaggcgcg ggatagatag accgctcggg aacgcttctc tctacatccc gaggcctttc
      421 tgccccttct tccaggagaa gatcttcgag cggatctact gcgggctgat cccggtggac
      481 ggttcggcat tccagctcga ctacatcttc atcgctgatg accgcgcctg catgatcgtc
      541 taccgccagg acggtctccg aaacgccgtg gtggtcaccc tgccgttcat aacctgcgtc
      601 cagagagagt ttgtgaaccg gatgatcgca aacgccgact gtatcggcgg ccagaagccg
      661 tccggtggat gaaggcggtt tcaatccggc cgggtggact ccagcggtat gtagggggtt
      721 aaggccagga cgtcctttct gatggcatca aacccgaccc gttcgacgaa ccgtgcagtc
      781 cgttctcccc gttctgcgtt gctgcggtag tactccagga gccggcggac caggtcgagc
      841 gcctcatccg ttgtgaggtc ttttgcgacg agatccccga tccgggggcg ggcgccggcg
      901 ttgccgccga agatgaccct ccagcctttt gccgtcccga gaagcccgat gtcccggagg
      961 taactctcgc cgcaggagcg ggggcagcct gagacaccga tcttgacctt tgccggcatg
     1021 ttcaggtcct ggtagatctc ctcgatcttg agcccgagac ccagcgagtc ctgtttgccg
     1081 tacctgcagg tctcggtccc ggggcatgcc tggacgtagt gaacgcaggg ggctgttgcc
     1141 tggcccacgg tcatcccgag ttcgttccag atcttctcga catcctcttc ctttatgccc
     1201 acgagcacca tccgctgccc ggaggtcatc ttgatgatcg ggatgctgta gcgccggacg
     1261 accttgatga tattctcaag gtcctcgggg ctgacgatcc cggcgggggt tctcggcacg
     1321 atcgcgtatg tggcgccgtc tctctggagg attgctcctc tgggttgatt ctccatcttt
     1381 gtcccatcct ttccggcggc atgcggggcc ttgcgccgga tacgatccag ccattgttcc
     1441 tggagaagga aaaacctgct gtcacgct
//
LOCUS       DAOSZX010000174         1396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_68573_length_1396_cov_22.236391, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000174 DAOSZX010000000
VERSION     DAOSZX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68573_length_1396_cov_22.236391"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..>1396)
                     /locus_tag="PK196_08415"
     CDS             complement(370..>1396)
                     /locus_tag="PK196_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41472.1"
                     /translation="ASAFNIKTETINPATGPLEPGQQVTARYVLTYDMATTDDLSDET
                     FELSTGLQNPSWDITVYRDGIAVYTTKKTGYYPVLTEFEISYGEGDTELDLQLRGTVP
                     SSTTGQVLIIKIEHLRGSDVKDSHSVTRDVVSAADIEGALAAQQQRLKDLKTDMDARA
                     AEGVDISAVQAKYNAASSALTSAASAGPAQAKSYIDTAKSNIDEAIKLLDKAWAEKAV
                     ADTGAAIESLDGMINYFVENRSMGSDPQVVAIMTKRESAVQFYTQAQDYLNAGNYAMA
                     RSKATDGLNKANEALADATALREKIGEGFSLSGNLLLFIGIGVAVVLIVVGVVLLRKR
                     KRWDELG"
BASE COUNT          270 a          409 c          384 g          333 t
ORIGIN      
        1 cccccaccgt gtctgtcgat ggagaatcgc catccctgcc ttctgcatat cctggtaaac
       61 gtctgccatc gaacactact cccgccaccc gatcgctctc gcggacacac ctcccacctc
      121 tgaaaaatct ttctcgactc aaacattgcg catccgttaa caccacccag cctcgcaaaa
      181 tgtttgcagt ctattttagc cccggggggc aaaggcccgt ttcagggcaa aaacgtcact
      241 tagaaacaaa gagatgcggc gaaagagggc aggtttgtgt tgtggttggt gaaccagccc
      301 tttttgcaaa ggtgtgagaa gggagtgtgt ggaaaaagat gtgggtcaat gatctgaggg
      361 tcaatgatct tatccaagtt cgtcccagcg ttttcttttc cggagcagca caacacccac
      421 aacgataaga actacagcaa ccccgatccc gatgaagagg aggaggttgc cgctgaggct
      481 gaacccctca ccgatcttct cccggagcgc ggttgcgtct gccagtgcct cgtttgcctt
      541 gttcaggccg tccgtagcct ttgagcgcgc cattgcgtag ttgccggcgt tcaggtagtc
      601 ctgtgcctgg gtgtagaact ggacggcgct ctcgcgcttg gtcatgatgg caacaacctg
      661 cgggtcgctc cccatggacc tgttctcgac aaagtagttt atcatcccgt caagcgactc
      721 gattgccgca ccggtatcgg caaccgcctt ctccgcccat gctttgtcca ggagtttgat
      781 cgcttcatcg atattcgact tcgcggtgtc gatgtaagat tttgcctgtg ccggccctgc
      841 cgatgcggcg ctggtgagcg cactgcttgc agcattatac tttgcctgga ccgcagatat
      901 gtccacgccc tctgccgccc ttgcatccat atcggtcttc aggtctttga ggcgctgctg
      961 ctgggcggca agcgcaccct cgatatcggc agcgctcacg acgtcgcgtg tcaccgaatg
     1021 ggagtctttc acatcactgc cgcgcaggtg ctcgatcttg attatcagaa cctgcccggt
     1081 cgtgctcgag gggacggtgc cgcgcaactg tagatcaagt tcggtgtcgc cctcaccgta
     1141 cgatatctca aactccgtca gaacgggata gtaaccggtt tttttggtgg tgtagacggc
     1201 aatcccatcg cgatagacgg tgatgtccca ggaggggttc tgcagccccg tgctcaactc
     1261 gaacgtctca tcgctcaggt cgtccgtggt ggccatatca taggtgagca cgtagcgtgc
     1321 ggtcacctgc tgccccggct cgagtgggcc ggtggcaggg ttgatggtct cggttttaat
     1381 gttaaacgcc gatgcc
//
LOCUS       DAOSZX010000175         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_70043_length_1375_cov_14.560606, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000175 DAOSZX010000000
VERSION     DAOSZX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70043_length_1375_cov_14.560606"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..435)
                     /locus_tag="PK196_08420"
     CDS             complement(166..435)
                     /locus_tag="PK196_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41473.1"
                     /translation="MSNSRIYYDVDREEAARFRFAALSEPADDRYDIQIFSHLPLYVR
                     TEVLRGKVIYCPDERFLYDVACQTIREFDDFKHRLYDYIGKEAMV"
     gene            complement(428..553)
                     /locus_tag="PK196_08425"
     CDS             complement(428..553)
                     /locus_tag="PK196_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41474.1"
                     /translation="MNHFVRHALERLKIVEGFEKVRITILYGSVAEGRARAGSDV"
     gene            complement(937..1098)
                     /locus_tag="PK196_08430"
     CDS             complement(937..1098)
                     /locus_tag="PK196_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41475.1"
                     /translation="MPGLPRVFAAGRTLEECRRNLAGVIGERFIIRLRAAPWVGCRSI
                     GPDPHRATS"
BASE COUNT          268 a          412 c          400 g          295 t
ORIGIN      
        1 caattccttg atctaatcga ctgccgactc gatgaagcgg ggttgattgc agtatataag
       61 gagatgcacc tattatgacg ctggacgtat cgactgaatg gggaggcgct ctttgaccaa
      121 tctcctggag tttgatacgg attgccgcat ccaggacctt gcgcgtcaca ccatcgcctc
      181 cttaccgatg tagtcgtaca atcggtgctt gaaatcgtcg aattcccgga tcgtctgaca
      241 agcgacgtcg tagaggaacc gctcgtcggg acagtagatt acctttccgc ggaggacttc
      301 cgtccggaca tagagcggca ggtgagagaa gatctggatg tcgtagcggt cgtcggcggg
      361 ttctgagagc gccgcaaacc tgaaccgggc tgcctcctcg cggtccacgt cgtagtagat
      421 gcgagagtta gacatcagac cctgccctcg cccgcccttc ggcaacggag ccgtaaagga
      481 tggtgatgcg gaccttctcg aaaccctcga cgatcttgag ccgctcaagt gcgtgccgga
      541 caaaatgatt cactcgctct caccccccgg gcgcgcccgg aagttctgta ggttctagat
      601 ggagatcgct ctcacattaa cgttttccgc ggcggggggg cggatcggac cggccgtctg
      661 tcccattctc ccgcaagacc aatacaccat ccgggatccc cgggcaatcg ctcagtttca
      721 caaccacaaa ataaaatgaa ataaaccgct acctggcgag ccgattccat tcttcacggg
      781 aaatgcctgc ctgccggagg atacgcacga gcaggtcgac gctgatctct ggcctatgtg
      841 gatttgggat ggtcagaacg aggtcggcct ctcaccatga ggggggtgtt tgcctcccct
      901 ggtatggccg ctcaaaaccc ggagtctgaa ggttcttcac gaggtggctc tatgtgggtc
      961 tggtcctatg ctccggcacc ctacccacgg tgcggcccgg agccgtatga tgaaccgttc
     1021 gccgatgacg ccggcaaggt ttctcctgca ctcttccagc gtcctgccgg ctgcaaagac
     1081 ccggggaagt cccggcacct ctccgtaata cggtttggcg tcctcgatga tcccgtatct
     1141 cgcgtgttcg agtgccgcgc ggatatacta ctcagggatc acggcgcagt tcagcaggat
     1201 tgcccccgca tatagtgttt cccgggtcga gcgggatcac gaccctgcta tcgggcgcat
     1261 tcggttggtg ccggtcatcc ccgatctgct gcgggtgcga ttcactcccc ggcacggtat
     1321 tcttcccgga gccgggcaaa gacccgcacc tggctgaggt agaccgccgg gtcgc
//
LOCUS       DAOSZX010000176         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_71727_length_1352_cov_21.646877, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000176 DAOSZX010000000
VERSION     DAOSZX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71727_length_1352_cov_21.646877"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1342)
                     /gene="purB"
                     /locus_tag="PK196_08435"
     CDS             complement(2..1342)
                     /gene="purB"
                     /locus_tag="PK196_08435"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HON41476.1"
                     /translation="MAIHPIDYRYGTPEMRAVWSEENRFRAIVTVEVALARAEAAHGM
                     IPPADAETIAACAGRARPERAKEIEAEINHDMMAIVKTIAEVCGDAGRWIHYGATSND
                     ILDTATALQIRESLSLIEEKLQRLLSVLLDRSEETKTLVCAGRTHGQIGVPTTYGLRF
                     AIWAAEVARHIERLRQMRPRVVVGQLTGAVGTQAALGDAGIEIQETMMEILGIGSVDV
                     SNQVVSRDRYAEYFMLLANVATTLDKIGLEIRLMQRSEIGELAEPFGKRQVGSSTMPH
                     KRNPIRSEQVCGLARIVRSAVEPALLNNVLWDERDLTNSAPERVIFPEASVLTDHILK
                     VMIEVLKGLEINHERIRRNLMLLRGVNLAESVMIELTKRGMNRQEAHEVMRTASMEAL
                     ARDRDLGQVLRERPEVTRFISPGELDALLDPKAYTGTAVRQVDRLIEKLRPLCS"
BASE COUNT          222 a          461 c          398 g          271 t
ORIGIN      
        1 ctcacgagca gagcggccgg agtttctcga tcaaccggtc cacctgccgt accgccgtcc
       61 cggtataggc tttcgggtcg aggagagcgt ccagttcacc ggggctgatg aacctcgtca
      121 cctccggccg ctcgcggagc acctggccaa ggtcgcggtc acgggcaagg gcctccatgc
      181 tcgccgtcct cataacctca tgagcctcct gccggttcat gccgcgttta gtcagttcga
      241 tcatcaccga ctcggcaagg ttcacgccgc ggagcagcat caggttcctc ctgatcctct
      301 catggttgat ctcgagccct ttcagcacct cgatcatcac tttaagtatg tggtcggtca
      361 gcaccgacgc ctcggggaag atcacccgtt cgggagcgga gttcgtcagg tcgcgttcgt
      421 cccagaggac gttgtttaac agcgccggtt cgaccgcgga tcgaacgatc ctggcaagcc
      481 cgcagacctg ctcgctcctg atagggtttc gcttgtgcgg catcgtgctt gaacccacct
      541 gcctcttccc gaacggctcc gcgagttcac cgatctcgga gcgctgcatc agccggatct
      601 caagcccgat cttgtcaagc gttgtcgcga cgtttgcaag gagcatgaag tactccgcgt
      661 agcgatcgcg cgagacaacc tggttcgaga cgtccaccga tccgatcccc aggatctcca
      721 tcatcgtctc ctggatctct ataccggcat cacccagcgc cgcctgcgtc cccaccgctc
      781 ccgtgagctg cccgacgaca acccgggggc gcatctggcg gagccgctcg atgtggcgcg
      841 ccacctcggc agcccagatc gcgaaccgga gaccgtatgt cgtcgggaca ccgatctggc
      901 cgtgggtgcg tccggcgcag acaagggtct tcgtctcctc gctccggtca aggaggactg
      961 agaggagccg ctgaagtttc tcctcgatca gtgataggct ctcgcggatc tggagcgcgg
     1021 ttgcggtatc caggatatcg tttgaggtcg ctccgtagtg gatccagcgg ccggcgtcac
     1081 cgcagacctc ggcaatcgtc ttgacgatcg ccatcatatc gtggttgatc tcggcctcga
     1141 tctcctttgc ccgctccggc cgcgcccgcc ccgcgcaggc cgcgatcgtc tctgcatccg
     1201 cgggcgggat catcccgtgc gcagcctcgg cacgggccag cgccacctcg accgtgacga
     1261 tcgcccgaaa acggttctcc tcgctccaga cggcacgcat ctccggcgtg ccgtagcggt
     1321 aatcgatggg atggattgcc atggttggta cc
//
LOCUS       DAOSZX010000177         1345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_72263_length_1345_cov_21.389147, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000177 DAOSZX010000000
VERSION     DAOSZX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72263_length_1345_cov_21.389147"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..>1345)
                     /locus_tag="PK196_08440"
     CDS             complement(234..>1345)
                     /locus_tag="PK196_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HON41477.1"
                     /translation="LAVKKGVDIYDAVPTLITDPADMAETAGSLHCGTLLLSKLLKRY
                     LDGAENMRIGITVKGCDAMGLYELAKRNQVNLDNVLMIGVNCGGSVSPVTARKMIREK
                     FGVDPNDVVKEEIDKGQFIIQTKDGQHKGISMDELEDEGYGRRPNCRRCKMKVPRQAD
                     LACGNWGVIGDKAGKATFVEVCSDKGADLLNRAVKAGAIATEPANPKGVEIRGKVENS
                     MLKLGDKWRKRYFEGLGEGKERLQKIMEESSRCTKCYACIEHCPICYCVECSTKKSYL
                     VPPGVLPVPFMFHLIRYAHVADSCINCGQCEEDCPMEIPNSLYMHSLQVEMEKMFGHT
                     PGVNLELPVLALVEEHAERERLASTGSDQIFDIFK"
BASE COUNT          259 a          417 c          379 g          290 t
ORIGIN      
        1 aacatgattt gcccaccctg tgtacgccgt gcgcctgatc gatcctttcg gaagatcatg
       61 cgcccggaaa gaccaatatc ataccggtat acacagcaga gaaaagacct ttacaacatc
      121 acaacggccg gaggcagaaa cgatcttcgg ttctcgtgaa ccgggggtcg gatgggggcg
      181 accgaccctt aagccagccg cccattcgaa accgccggac gacccacccg tagttacttg
      241 aagatatcga agatctggtc gcttccggtt gaggcgagcc gttcccgctc ggcatgctcc
      301 tcaacaagcg caagcaccgg gagttcgagg ttcacgccgg gtgtgtgacc aaacatcttc
      361 tccatctcga cctgcaggga atgcatgtag agcgagttcg ggatctccat cgggcagtct
      421 tcctcgcatt ggccgcagtt gatgcacgag tccgccacgt gggcgtagcg gatcaggtgg
      481 aacataaacg gcacggggag gactcccggc gggaccaggt aggacttctt cgtgctgcat
      541 tcgacgcagt agcagatcgg acagtgctcg atgcaggcgt agcacttggt gcaccgtgac
      601 gactcttcca tgatcttctg gaggcgctcc ttgccctcgc ccagaccctc gaagtaacgc
      661 ttccgccact tgtcgccgag tttcagcatc gagttctcga ccttgccgcg gatctcaacg
      721 cccttagggt ttgcaggctc ggttgcaatg gctccggctt tcacggccct gttcaggagg
      781 tcggcgccct tatcggagca gacctcaacg aaggtcgcct ttccggcctt atcgcctatg
      841 acaccccagt tgccgcaggc aaggtcagcc tggcgcggga ccttcatctt gcagcggcgg
      901 cagttggggc gacggccgta tccctcgtcc tcgagttcgt ccatcgagat gcccttgtgc
      961 tggccatctt tcgtctggat gatgaactga cccttgtcga tctcttcctt gacaacatca
     1021 ttcgggtcga ccccgaactt ctcgcggatc atcttacggg cggtcaccgg gctgacagac
     1081 ccgccgcagt tgacaccgat catcaggacg ttgtccaggt tgacctggtt gcgctttgcc
     1141 agttcgtaga gccccatcgc gtcgcagccc ttcacagtga tcccgatgcg catattctct
     1201 gcgccgtcaa ggtacctctt gagaagtttc gagagcagaa gcgtcccgca gtggagcgag
     1261 cctgccgtct cagccatatc tgcagggtca gtgatcaggg tcgggacggc gtcgtagatg
     1321 tccacacctt tcttcaccgc cagca
//
LOCUS       DAOSZX010000178         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_75989_length_1297_cov_12.231079, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000178 DAOSZX010000000
VERSION     DAOSZX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75989_length_1297_cov_12.231079"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PK196_08445"
     CDS             <1..1202
                     /locus_tag="PK196_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41478.1"
                     /translation="IVLSLLCCLVPGASAVSISISPSQIQEGDTITVSYSGLSNGSAF
                     ALRMESLVDVAGETSFTYQANQVSMPFGLNSPQVVLSASPVTEAGIEASEGETIKRIT
                     QISRTGSVSISQSLGNLPAGTMELIKAFGTPEEGVDAVNVVLEFSGVKMGPDSGAITF
                     SLTGITDGSAHVIALVDGNIVTDQTILVGNPTITTPTTSPTTPPPSGGSGGSSGGGSS
                     GGGATETPTAAPETVTVSSLDGIVTLETVDDAIDGAAPADLRIIRSTPSGVPDEWQVL
                     AGSYIISPSAATFDPAAALSFNLSGVNATTPFIAVYRDGAWTVVPSRYEGETRLAATV
                     SSGGQYALMAYAAVGAGDDTTPVETETAPMTTTEAAATTTPATAWGTAGVIGALAVAV
                     ALRRQRP"
BASE COUNT          247 a          438 c          377 g          235 t
ORIGIN      
        1 tcatagttct ctctctcctc tgttgcctgg ttccgggcgc ctctgccgtc tccatctcca
       61 tcagcccgtc ccagatccag gagggggata ccatcaccgt ctcctacagc ggtctctcca
      121 acgggagcgc ctttgccctc cggatggagt cgctggtgga tgtcgcaggg gagacctcgt
      181 ttacctacca ggcaaaccag gtcagcatgc ccttcggcct gaacagtcct caggttgttc
      241 tcagtgcttc cccggtcacg gaagccggga tcgaggcctc cgagggggag accatcaaac
      301 gaataaccca gatcagcagg acaggcagcg tatcgatcag ccagagcctt ggcaacctcc
      361 ccgccggcac gatggaactc atcaaggcgt tcgggacacc ggaagaaggt gtcgatgccg
      421 tgaacgttgt tcttgagttc tccggggtca agatgggccc ggattcgggg gcgatcacct
      481 tcagcctcac cggtatcacc gacggctccg cacatgtcat tgcccttgtg gatggaaaca
      541 tcgtgaccga ccagacgatc cttgtcggca accccacaat caccacaccg acaacatctc
      601 ccaccacccc tccgcccagc ggtggttctg gcggcagttc cggcggcggc agttccggtg
      661 gcggcgcaac ggagacaccg accgccgcgc ctgaaaccgt gacggtctcc tcgcttgatg
      721 ggattgtcac ccttgagacc gtggatgatg cgatagatgg tgcggcgcct gctgacctgc
      781 ggatcatcag gagcacaccc tcaggggttc cagatgagtg gcaggttctt gccggatcat
      841 acatcatctc cccgtcagcc gccacatttg acccggccgc cgccctatcg ttcaacctct
      901 cgggcgtgaa cgcaaccacg ccgttcatcg cggtataccg ggacggggcg tggacggtgg
      961 tgccctcccg gtatgagggg gagacgcgcc tcgccgccac ggtctcaagc ggcggtcagt
     1021 atgcccttat ggcctacgcg gcagtcgggg caggggatga taccacgccc gtggagacag
     1081 aaaccgctcc aatgaccaca actgaggctg cggccaccac caccccggca acggcctggg
     1141 gaaccgccgg cgtcataggc gcccttgcgg tcgcggtcgc cctacgcagg caaagaccct
     1201 gaaccgggca gcacgcccgg tcacactaat tttacggcgg gttcgcaatc tccagaaccc
     1261 gggttttgtg gagcaacaag cctctcaacc cctcacc
//
LOCUS       DAOSZX010000179         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_77819_length_1275_cov_8.937705, whole genome shotgun sequence.
ACCESSION   DAOSZX010000179 DAOSZX010000000
VERSION     DAOSZX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77819_length_1275_cov_8.937705"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1063
                     /locus_tag="PK196_08450"
     CDS             47..1063
                     /locus_tag="PK196_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HON41479.1"
                     /translation="MKFLTPLLLASLLVIPSSSGIYIASPDYVSSIASADVKDVINGR
                     YGDVIFATSSGLSIYTADGRWYSVNARNPRQTDYGFLEPLDNMVIALALDPDGHLWLG
                     YSCGLQIGNGTGYHAIQDQDHLKNLNINCIRRWDNEVWVAAGSAGLHRWHDGNWTWYK
                     PGGPSGAGFYTVVSMVVDAESDALVIDSENEGVWVLKGHARDRRFEQVFFGDDPLLTV
                     SGVRVDPFGGVYLFNATTILHYSPDEGVTPVLNATDLSTFPVTINDVAATRDGTLLVA
                     SDKGIYGLNSSGIVLHLTSRDGIRENFVKELFVDASGRCWFVVPGHVGYIPLIPQYPT
                     IPVA"
BASE COUNT          246 a          363 c          384 g          282 t
ORIGIN      
        1 aaggctgcct catcatcaag agggcggatg ggacatgcag ccgggtatga aattcctcac
       61 ccctcttctt ctggcatctc tcctggtcat tccctcctct tctggtatat atatcgcgtc
      121 tcctgactac gtctcctcga tcgcctctgc ggacgtaaag gacgtgatca atgggcggta
      181 cggggatgtc atctttgcga cctccagcgg tctctcaatc tacacggcag acgggaggtg
      241 gtactcggtc aacgccagga acccacgcca gacggactac ggttttctcg aaccgctcga
      301 taacatggtg attgctcttg ccctcgatcc agacggtcac ctctggctcg ggtattcctg
      361 cgggctgcag atcgggaacg ggacggggta tcatgcaatc caggaccagg atcacctcaa
      421 gaacctgaac ataaactgca tcaggcggtg ggataatgag gtctgggtcg ccgcagggag
      481 cgccggtctc caccgctggc atgacgggaa ctggacgtgg tataaaccgg gcgggccatc
      541 gggtgcagga ttttatacag ttgtgagcat ggtggtcgat gccgagagcg atgcgcttgt
      601 catcgactcc gagaacgagg gggtgtgggt gctgaaaggc catgcgcggg atcgccggtt
      661 tgaacaggtc ttcttcggcg acgatccgct gctcacagtc tccggggtgc gtgtcgaccc
      721 ctttggcggg gtctacctgt tcaacgctac aaccatcctc cattactctc ctgatgaagg
      781 tgtcacccct gttctcaacg ccacggacct gagcaccttc ccggtcacca tcaacgacgt
      841 tgcggcaaca cgcgacggga cgcttcttgt tgcgtccgat aaaggtatat acgggctgaa
      901 ctcttccggc atcgtgctgc atctcacgtc tcgggacggt atccgggaaa actttgtcaa
      961 ggaactcttc gtcgacgctt ctgggcggtg ctggtttgtt gttccagggc acgttggcta
     1021 catcccgctg atcccgcaat acccgacaat tccggtggcc tgacggccgg aggggggaga
     1081 gcggaggggg tgttctgcag gagaaaggga tgccgtggca tggatgaccc cctcctttgc
     1141 gctcacttcc ccttacggat gaatcttcca acgcggaatg gagttgtacc cggcacagaa
     1201 gccgggaaag tgccttcggt cgcccgccgg acgtcctcga tcgagtagaa cgccctgggg
     1261 ttgaactgat ggatg
//
LOCUS       DAOSZX010000180         1234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_81437_length_1234_cov_12.943172, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000180 DAOSZX010000000
VERSION     DAOSZX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81437_length_1234_cov_12.943172"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PK196_08455"
     CDS             <1..701
                     /locus_tag="PK196_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HON41480.1"
                     /translation="YYLIYRRRGARFIDDQQARLLFVLIAIGIAVITWDLVMLSELDL
                     PTALHQALFMTAAAVSSTGFQVVSPNAWASVTVLFLAMLVLIGGSSGSTAGGIKLSRV
                     VLGFQSLVWWFRRMFVSGKVLVPFRHEGRVIPKNVADLEISRNMLVIMLYLLIIFVST
                     ILVMHLQPTSFDSSNVIFDVTSAMCNNGISTGFVSPDMDSAAKALFIFVMWIGRLEII
                     PVIMLFTGLFKRFD"
BASE COUNT          222 a          338 c          365 g          309 t
ORIGIN      
        1 tctactacct catctaccgg aggagaggtg cacggttcat cgatgatcag caggcgcgcc
       61 tcctcttcgt cttaatcgcc atcgggatcg ctgtgatcac ctgggacctt gtcatgctca
      121 gtgaactcga tctccccacg gccctccatc aggcgctctt catgaccgcc gcagctgtat
      181 ccagtaccgg gtttcaagta gtctccccga acgcgtgggc aagcgtgact gttcttttcc
      241 ttgcgatgct ggtgctgatc ggcgggtctt cggggagcac cgcgggaggt atcaaactct
      301 ccagggtggt tctggggttc cagagcctgg tctggtggtt ccggcggatg ttcgtttcgg
      361 ggaaggtgct tgtgccgttc aggcatgagg ggcgcgtgat cccgaagaac gttgccgatc
      421 ttgagatctc ccggaacatg ctggtcataa tgctctacct cctgatcatc tttgtatcaa
      481 ccatactcgt gatgcacctc cagccaacgt cttttgattc gagtaacgtg atcttcgatg
      541 ttacctctgc aatgtgcaac aacggtatct ctactggttt tgtctcgccg gatatggact
      601 ctgcagcaaa ggccctcttc atctttgtca tgtggatagg gcgtcttgag atcatcccgg
      661 tgataatgct ctttacgggt ctcttcaagc ggttcgattg agagcgggct ggctctcgat
      721 tgaatgatca gggttgtatc cctcctctca ccccggatgc agtcagaact ggctgagcgg
      781 ccgggctttc gtgcaacccg gtcaggcggg aaaatctctc agagaccctc aaaccaactc
      841 ccctcggact cctgccgggt ttgatggagc ctcaccatgg acggagatcc ccctttgcga
      901 tcccgtctcc gccccccggc acgggttccg tgggccaatc gtcagacact tgcccgctcc
      961 tttgaggagg ggtggggctg gtcgacgaga gacgtccaac gagagtgact cggttgcatc
     1021 gctgaaccgg tttgatcgtc cattgcgcat gctgccgggg aaaggtggag tgtttgtagt
     1081 ccggatcgtt aattcgatga gttactctcg gaggtgcggt tagagagagg gggattttcg
     1141 tggctcactg atgtatgggg ctggccccgg atgcgaagta ggaaaagagt cagcaccctc
     1201 ctgacgattg gccggtgtca gggtcggccg gaaa
//
LOCUS       DAOSZX010000181         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_82473_length_1223_cov_11.715753, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000181 DAOSZX010000000
VERSION     DAOSZX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82473_length_1223_cov_11.715753"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..554
                     /locus_tag="PK196_08460"
                     /pseudo
     CDS             228..554
                     /locus_tag="PK196_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038640.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
     gene            557..892
                     /locus_tag="PK196_08465"
     CDS             557..892
                     /locus_tag="PK196_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON41481.1"
                     /translation="MIFDDIRLLISPIELQIPYKLYLSSDKDIEDAVYLWVLFREMLD
                     GDLLQVQGEPYGIEFIYSDEDVGFIAVIPELPGCPAFGVTGEEGRVIPEPAGRERLPD
                     IPAGKGTTS"
BASE COUNT          231 a          357 c          392 g          243 t
ORIGIN      
        1 ttgtgacgaa ggaatacctg ccgacaaggc tgtcggagat gggggttatc tagagagggg
       61 tgcccgtggt tattgcccgg gcccaccctt ccccacccac gggcacctct gttttcccaa
      121 gttcctcccc ggagccgctc ccggatgcgg atttccccgg cgtatcgtca tgcgctgccc
      181 cccggagcgc gggcggagtg gggctcgctg ccaggatctt caaccgggat gagatggtcg
      241 actacgttct caggtgcggg cgaactcggt gtgaaggccc ttgccctcct cgataccgcg
      301 aacaccacag cctacgggac gccccggatc acgacggtca gcaccgcggc atgcacacgc
      361 ccgggtatcc tcgtcaccgg ccacgacctc aaagacctcg ccgacctcct tgagagcaga
      421 ccgcgggggc gggggtggac gcctacaccc acggcgagat gctgccggcg cacgcctacc
      481 ccggtcttgc gaagtatgat caccttgtcg ggaattatgg tggctcctgg cccttccagc
      541 gggaggagtc cgagcggtga tcttcgacga tatccggctt cttatatcgc ccatcgagct
      601 gcagatcccc tacaaactct atctttcgag cgacaaggat attgaagatg ccgtctacct
      661 ctgggtgttg ttccgggaga tgctggacgg ggatctcctg caggtccagg gggagccgta
      721 tggaattgag ttcatctaca gcgatgagga tgtggggttc atcgccgtga tcccggaact
      781 tccggggtgc cctgccttcg gcgtgaccgg ggaggagggg agagttatcc cggaacctgc
      841 cggcagggag cgtctcccgg atatccctgc cgggaaaggc accacgtcat gaccgtgagc
      901 agccccttat aatacgcagg cgatctccag aaatcgggtg attgcagggc cagcgcacgg
      961 tatttcaacc ttcggtaggg gcgctgtact atacctggct ctggccaggg aaagggttaa
     1021 tgaggtggga ggaagcaatc ctcgttgctg gcagatctga ttatgcgtgg aaacgaaccc
     1081 ttgtggaggc cgggaggtgg aaagagagat gggtgatatc gagagagagc ggatcatcga
     1141 ccagctccgc cgatgcctcg ccgacaagga ggatgtattg ctcgggtacc tctacggctc
     1201 ttttctctgc cgcgatgagt tcc
//
LOCUS       DAOSZX010000182         1209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_83836_length_1209_cov_19.975737, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000182 DAOSZX010000000
VERSION     DAOSZX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83836_length_1209_cov_19.975737"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..779)
                     /locus_tag="PK196_08470"
     CDS             complement(162..779)
                     /locus_tag="PK196_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HON41482.1"
                     /translation="MTQSYEDLLKKAYTNITEPTEFEDRFTVPAARVFIEGKTTVLEN
                     FAEITSTLRRDQDHLMKHLLGELGTAGKIDGTRAIFSGKFEQEQINSIIKGYVDDYVI
                     CSECGKPDTRLVKTERVLMLQCDACGGHRPVRKRKATVESSEGAKPTEGAIMDVTPQF
                     LSKRGDGVVKLGRYTMYVANAKPGQTVKVKITRIAGTIIFTERLD"
     gene            872..>1209
                     /locus_tag="PK196_08475"
     CDS             872..>1209
                     /locus_tag="PK196_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HON41483.1"
                     /translation="MLPAQDLALLRKTLKRELTDVELACFENLWSEHCSYRSTRHLLR
                     TLPTEGPGVLLGPGDDAAIVQFSDTCALAVGMESHNHPSYVDPYDGAATGVGGIVRDV
                     LSMGARPIAL"
BASE COUNT          226 a          369 c          348 g          266 t
ORIGIN      
        1 cggcgcaacc ccaactggga tcgcgacgtt gcacggagga gcgggcaggc gacccctgat
       61 cagctccgcc gggaacttgc ggcaagcgct gttgcctgct cccggatacg tgaagaggaa
      121 gcagggttct gggtggagat ccgaaaggga tccggctgct tttaatctaa acgctcggtg
      181 aagatgatcg tcccggcgat cctggtgatc ttcaccttca ctgtctgacc cggttttgcg
      241 tttgcaacat acatggtgta gcggccgagt tttaccaccc catcgccgcg cttcgagagg
      301 aactgcgggg tgacatccat gatagcgcct tccgtcggtt ttgccccttc ggaggattct
      361 accgttgcct tccgcttccg gacgggtcgg tgcccgccgc aggcgtcgca ctggagcatc
      421 agaacccgtt cggtctttac gaggcgggta tcgggcttcc cgcactccga gcagatgaca
      481 tagtcatcta cgtaaccttt tatgatggag ttgatctgct cctgctcgaa ctttccggag
      541 aatatggcac gcgtcccatc gatcttaccg gcggtgccga gttcgccgag gaggtgtttc
      601 atcaggtggt cctggtcgcg ccgcagtgta ctcgtgatct cggcgaaatt ctcaagaacc
      661 gtggtcttcc cctcgataaa gaccctggcg gccggaacgg tgaaccggtc ctcaaactcc
      721 gttggctccg tgatgttggt atacgccttc ttcaaaaggt cctcatacga ctgggtcata
      781 cctcactatg gatcgcgcaa actcaaaaag tggttccccg gcgtgacctt gcgcatgctt
      841 atctaggcag gcaacccacc agtcttgcag tatgttgccg gcacaggacc tcgcacttct
      901 tcgaaagacc ctcaaacgcg aattaaccga tgtcgaactt gcctgctttg agaacctctg
      961 gagtgagcac tgcagttatc ggtccaccag acacctcctg cggacgctgc ccacagaggg
     1021 gcccggcgtg ctgcttggcc cgggggacga cgctgcaatc gttcagttca gcgacacctg
     1081 tgccctcgcc gtcgggatgg agagccacaa ccatccaagt tacgtcgacc cctacgacgg
     1141 cgccgcgacg ggtgtgggcg ggatcgtccg cgacgttctc tcgatgggtg cccgccccat
     1201 agcccttat
//
LOCUS       DAOSZX010000183         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_84719_length_1200_cov_20.434061, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000183 DAOSZX010000000
VERSION     DAOSZX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84719_length_1200_cov_20.434061"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK196_08480"
     CDS             complement(<1..1143)
                     /locus_tag="PK196_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HON41484.1"
                     /translation="MPLVDGFDDTTILSIGHLGIVAGAYDSLQIADVIDTALPKTRHH
                     HLSHSQVLKAMILNGLGFIERRLYLFPDFFDDIAVERLLGEGITRQHLNDDVLGRTLD
                     AIAAYGPTELFNEIVAKCLLQTEFGSHCVHIDTTNFSVTGAYESDLNMGEIEITYGHP
                     KDGRWDLKRFVLGIASNQYGVPLFLQTFSGNESDKETILTIIKQLKENLKSAEKVYHV
                     ADSAFYTAANLQSLGQHTFWISRVPATIKEVRDLVRTGEPFLSCTDDRYTYQEHFSEY
                     AGVRQKWVLYRSVPMYEREEKTFEKNLAKDLDRAQTSLRKLCSREFACVPDARMAAEK
                     WLEQHLRYRFRDLEFVTITRKQEKKRGRPKNGEPVLLSYKITAGIEH"
BASE COUNT          246 a          303 c          362 g          289 t
ORIGIN      
        1 gtgctcgatc cctgccgtga tcttgtagga gagaaggacc ggttccccgt tcttcggcct
       61 cccccgcttc ttctcctgtt tccgggtaat cgtgacgaac tcgaggtcac gaaaccggta
      121 tcgcagatgt tgttccagcc atttctctgc agccatccgc gcatccggga cacaggcaaa
      181 ctcccgggaa cagagtttcc gaagagacgt ctgcgcccgg tccaggtcct ttgccaggtt
      241 cttctcgaac gtcttctcct cacgttcata catcggaact gaccggtaca gaacccattt
      301 ctgccggacc ccggcatact cactgaaatg ctcctggtag gtgtagcggt cgtcggtgca
      361 ggacaggaat ggttctcctg tccggaccag atcccgcact tccttgatcg tggccgggac
      421 ccggctgatc cagaaggtgt gttgccccag gctttgcagg ttggctgcag tgtaaaacgc
      481 agaatcggcc acatggtaga ctttttcggc agatttcagg ttttctttga gttgcttgat
      541 gatcgtgagg atggtctctt tgtcggattc gttgccggag aatgtctgga ggaagagcgg
      601 aaccccatac tggtttgagg ctattccgag gacaaaacgc ttgaggtccc accggccatc
      661 cttcggatgg ccgtaggtga tctcgatctc gcccatgttg aggtcggatt catacgcccc
      721 tgtgacgctg aagttggtgg tatcgatatg gacgcagtgt gatccgaatt ctgtttggag
      781 tagacatttt gccacgatct cgttgaataa ctcggttggg ccataggcag cgatcgcgtc
      841 cagggttcgg ccgagcacgt cgtcgttgag gtgttggcgc gtgatgccct ccccgaggag
      901 acgttcaacg gcgatgtcgt caaagaagtc ggggaagagg tagagacgac gttcaatgaa
      961 cccgagaccg ttgagaatca tggccttgag aacctgggag tggctgagat ggtgatgacg
     1021 ggtcttcggg agagcggtat cgatcacgtc ggcgatctgc agggagtcgt aggcaccggc
     1081 gactatgccg agatgaccga tggagaggat tgtggtgtcg tcaaaaccgt caacgagagg
     1141 catactgaag cgtattcgct gaaagtacaa agatcttgcg atcttgaata ttcagagtgg
//
LOCUS       DAOSZX010000184         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_84813_length_1199_cov_10.692308, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000184 DAOSZX010000000
VERSION     DAOSZX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84813_length_1199_cov_10.692308"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            491..>1199
                     /locus_tag="PK196_08485"
     CDS             491..>1199
                     /locus_tag="PK196_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON41485.1"
                     /translation="MAYQQENYLLVDGKQRAAYTVILLFGVVSLFGDIIYEGARSVAG
                     PYLYLLGAGALAVGVVAGLGEFLGYALRLASGYLADATRHYWAITFIGYGMLAAVPLL
                     ALAGSWEIAALLLILERVGKGIRVPARDAILSNATAAVGRGWGFAVHEALDQIGAIIG
                     PLLFAAIFLLDGDYRGGFALLAVPFVLMILALLFARRRVPEPVSLEEGGAVLREERSL
                     PRTLLPYGVFTALTMAGF"
BASE COUNT          199 a          366 c          359 g          275 t
ORIGIN      
        1 cgctggtgga tataattgtt cctcatgagg gttataggtt ttcggtgagc gagggccatc
       61 tggattttca tctgaaatgc tcccgccatc ttcatgcggc gtgcgtcctg gacacacggg
      121 cgtcatgtgg tcttcctccg tcaggcccct accccgcggt ggcgaccctc catcgatacg
      181 gtgtccgggg cagcatcact accgtcctgg tggatccctg atcaagatcc tgcttccgcc
      241 cctgcaacgg ccagcgtgac aatcgtccca cagcgccccg ccccctcgag ggggcggctg
      301 atcagccgca aaagacgttt caccgcggtg gttacggggg aggcgtcaag ccatgagcga
      361 actggtttgg tcccagcgcg taataatggg aaaaagaggg ggagtttata tcacagaacc
      421 gttgatttga tggcctctgg aaaaggtctc tcaaaaagac tccgggcagg tgtgcccatc
      481 aataaggctt atggcctatc aacaagagaa ttacctcctt gtggacggca aacagcgcgc
      541 agcctataca gtgatacttc tcttcggtgt tgtcagcctc tttggcgata taatctatga
      601 aggggcgcgg agcgttgccg ggccatatct ctatctcctg ggcgccggcg cccttgccgt
      661 cggggttgtg gcgggactcg gtgagtttct cggctacgcc ctccgtctgg cctcaggtta
      721 cctcgccgac gccacccggc actactgggc tatcaccttc atcggctacg ggatgctcgc
      781 cgcggttccg ctcctcgccc ttgccggttc atgggagatt gctgcgctcc tcctcatcct
      841 tgagcgggtg gggaaaggca tccgggtgcc tgcacgcgat gccatactct ctaacgccac
      901 cgcggcggtc ggcaggggct gggggtttgc cgtgcacgag gctctcgacc agattggggc
      961 tatcatcggg ccgcttttat tcgccgccat cttcctgctg gatggagatt accggggcgg
     1021 attcgcactc ctcgccgttc cgttcgtgct gatgattctt gctctcctct ttgccaggcg
     1081 cagggtgccg gaaccggtgt cacttgagga gggtggggct gtcctcagag aagagagatc
     1141 cctcccccgc accctgctac cctacggtgt cttcaccgcg ctcaccatgg ccgggttcg
//
LOCUS       DAOSZX010000185         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_87248_length_1175_cov_18.695536, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000185 DAOSZX010000000
VERSION     DAOSZX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87248_length_1175_cov_18.695536"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..>1175)
                     /locus_tag="PK196_08490"
     CDS             complement(371..>1175)
                     /locus_tag="PK196_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HON41486.1"
                     /translation="FSLIETVIILAIGSGAGVMVGIMGGSGVMVVVPMLTLLLSFPVH
                     TAIGTSLLIDVVATVVTATIYHRHQNVYIRPGLWIALGSVGGALAGSRVADMIPAVGM
                     SGLFALMLIPTGVYLWLKGLGRPGLAAAGGEIDSPRLPVQTRRQQFTALGLGVFVGMM
                     CGLFGAGGGGMILLILIFVLRYPVHLAVGTSSFIMMVTAASGTAGYIIHSNIDIQAAL
                     IASVPTVLAAGIGAGIANRVSERTLGRIIGVVLAALGVAMIATRVVAGG"
BASE COUNT          231 a          371 c          360 g          213 t
ORIGIN      
        1 agtctgcctc gtctccgatc cgctcgatgc agttcccgat gagaaaaagt tctttctgga
       61 atatgacatc cccccgaacg gcagagttgc ctcctgcatg taggaggtgg aggatcgcca
      121 gatccaggtc ggagaggctc tcagttatct catcgagggt gaaatccacg gtcatcttca
      181 acctcgctca tcggagtcgg gctgcccgcc ccgcatgtgc tgtgtaatag ttatggcata
      241 tagttgttat gcgtatgctt gtgtccatca gaactcacgg cgatgccatc cgggcggggt
      301 atcgatctga tggcctcatg ttacaccact aaataagccg ccggcatctt tttgacgggt
      361 gggtcttccc tcaccccccg gccacgaccc gcgttgcgat catggcaacc ccgagggccg
      421 ccaggacaac ccctatgatc ctgccaagcg tcctctcgct gaccctgttt gctatcccgg
      481 caccgatgcc ggcggcaagc acggtgggga cgcttgcaat cagcgctgcc tgtatatcga
      541 tgttggagtg gatgatgtag cccgccgtgc ccgatgcggc ggttaccatc attatgaacg
      601 aggatgtccc gaccgccagg tggacggggt agcggagcac aaatatcagg atcagcagga
      661 tcatcccccc gccaccggcg ccaaagagcc cgcacatcat gccgacgaag acaccgaggc
      721 caagcgcggt gaactgctgg cgccgggtct gcaccgggag ccggggcgag tcgatctcgc
      781 cgccggcggc ggcgaggccg ggacggccaa gccccttcag ccagaggtag accccggtcg
      841 ggatgagcat cagcgcgaag agcccgctca tcccgaccgc cggtatcata tcggcaaccc
      901 tgctccccgc cagggcgccg ccgaccgaac caagcgctat ccagagccct ggccggatgt
      961 agacgttctg gtggcggtgg tagatggtgg cggtcacgac cgttgctacg acgtcgatga
     1021 ggaggctggt gccgatcgcg gtgtggacgg ggaaagatag caggagcgtc agcatgggca
     1081 ccaccaccat caccccgctg ccccccatga tcccgaccat caccccggcg ccgctgccga
     1141 tggcaagtat gatgacggtc tcgatgaggg agaat
//
LOCUS       DAOSZX010000186         1173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_87437_length_1173_cov_16.345259, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000186 DAOSZX010000000
VERSION     DAOSZX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87437_length_1173_cov_16.345259"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..477
                     /locus_tag="PK196_08495"
     CDS             154..477
                     /locus_tag="PK196_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HON41487.1"
                     /translation="MAPVRIGGGGERKVRPVVVIRGGERGSLLVCPVSSTPSSDGVSI
                     PISLEDFASGGLDLFAESYVLAAHSATIQPADVAGKKGSLLPGTLAAIREVMTCLQRQ
                     AGHPP"
     gene            560..1144
                     /locus_tag="PK196_08500"
     CDS             560..1144
                     /locus_tag="PK196_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON41488.1"
                     /translation="MQTDKESTVISVIRDRRSIRNYTERDVPDDLLQTIIAAGIEAPT
                     AMGLQPWRFVVVKDRDLMRRVSDYCRPILAEIVGREGGPGSEEFLAALRSPGFSLFYN
                     APVLVLVLGSREAQSGVIDCALCAENMMLAAWALGIGSCWIGSAAIVEKNPALLAELK
                     VPDGYRIVAPLVFGYPAAIPPKPARREPLITWIR"
BASE COUNT          206 a          349 c          377 g          241 t
ORIGIN      
        1 ctgtatctcg attgggcaga cgcctcaccc ggagaccctc ttttccgcgt gctgcattcg
       61 ccgccgtcca atcgcctgcc gggatcgatt tatttattgt ttctgtcacg atagattact
      121 cgcatggggc gctacgccag cggggatctc gttttggcac cggtgcgcat aggaggcggc
      181 ggtgagcgga aggtgcgccc ggtggtggtc ataagaggtg gagagagggg gagcctgctg
      241 gtctgcccgg tatcgagcac cccgtccagc gacggcgtat cgatcccgat ctcgcttgaa
      301 gactttgcca gcggtggact cgacctcttt gcggagagtt acgtcctggc cgcacattct
      361 gcaaccatcc agccggcgga tgtcgccggt aagaaaggct cgctcctgcc cggcacgctt
      421 gcagccatcc gggaggtcat gacatgtctg cagaggcagg ccgggcaccc tccttgagtt
      481 gcaaatcagc ggcaaagtcc gctcacagcc ggcagaccgg gtgcagggta taaataccgc
      541 tggcggctac ccgtgtagca tgcagacaga taaagagtca acagtcatct ccgtgatccg
      601 ggatcgccgg agtattcgca actacaccga gagggacgtc ccggatgatc tgttgcagac
      661 tatcatcgct gcgggtattg aggcccccac cgccatgggg cttcagccct ggcggtttgt
      721 ggtggtgaaa gaccgcgacc tgatgcggcg cgtctcagat tactgcagac cgatcctggc
      781 agagattgtc ggcagagagg gcggccccgg gtctgaggag ttcctggcag cgctcaggag
      841 ccccgggttc agcctctttt acaacgcccc ggtgctggtt cttgtcctcg gttcaaggga
      901 ggcgcagtca ggtgtcatag actgcgccct ctgtgcggag aacatgatgc ttgccgcctg
      961 ggcgcttgga atcgggagtt gctggatcgg ttctgcggcc atagttgaga agaaccccgc
     1021 cctccttgca gagttgaagg ttccggacgg ctaccgcata gtggcgccgc tggtcttcgg
     1081 ctaccccgcc gccatcccgc cgaaaccggc gcggcgcgag cctctgataa cctggatccg
     1141 ctgatgcccg tgtcgtctgg aagccgcgtc tga
//
LOCUS       DAOSZX010000187         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_94418_length_1111_cov_19.285985, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000187 DAOSZX010000000
VERSION     DAOSZX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94418_length_1111_cov_19.285985"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1111)
                     /locus_tag="PK196_08505"
     CDS             complement(102..>1111)
                     /locus_tag="PK196_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON41489.1"
                     /translation="DKTQVFYLVENDHITHRVLHVQLVSKIGRTIGRALGLNEDLIEA
                     IALGHDIGHTPYGHLGEGYLDELCREYGIGGFHHNVQGVRFLESIEDCDLTLQVLDGV
                     LCHNGETHDRCLAPEGEADWDSFAAKFHRIEAGGDALPLTAEGCVVRCADSISYLGRD
                     LADALEVEVMDESELAAFPESCMAVFGIKRADKKEFSDINRVVLDVLIKDVITNSYDT
                     DLIAFSEAASESIVAFKEFNRHKIYENERLVRNREKIRFMFRYLFEQIIEDLNDERKD
                     SPVYVDFFDAPWVSSAYTATAKPAEAARDFIAGMTDRYFEWVFRHYILPERVRSRYRG
                     W"
BASE COUNT          213 a          336 c          310 g          252 t
ORIGIN      
        1 ggaaggttca ccccgtcgcg gcacaatctg acgggacatg tttgatggct ccacagcctc
       61 cagcgatcag cggtccggca ctctcgttgc ccgcgttttt cttaccatcc ccggtacctc
      121 gatctgaccc gctccgggag gatgtagtgc cggaagaccc actcaaagta gcggtcggtc
      181 atccctgcaa tgaaatcccg tgcagcctcc gctggttttg cggtggcggt gtaggcgctg
      241 gatacccatg gcgcatcaaa aaagtctaca taaactggtg aatctttgcg ttcatcattg
      301 aggtcctcga tgatctgctc gaagaggtag cggaacatga accggatctt ctcccggttc
      361 ctgacaagcc tctcgttctc gtagatctta tgcctgttga actccttgaa ggccacaata
      421 gactctgatg ctgcctccga gaatgcgatg aggtctgtat cataactgtt cgtgatgacg
      481 tcctttatta gaacgtcaag cactacccgg ttgatatcag agaactcctt cttatccgct
      541 ctctttatcc cgaaaactgc catgcaactc tcagggaagg cagcgagctc gctctcgtcc
      601 atcacctcaa cctcaagggc gtctgcaaga tcgcggccca ggtacgatat gctgtcggcg
      661 cacctgacga cgcacccttc ggcggtaaga gggagtgcgt cgcctccggc ctcgattcgg
      721 tggaattttg ccgcaaacga gtcccaatcg gcctcaccct cgggcgcaag gcaccggtcg
      781 tgggtctccc cgttgtggca gaggacaccg tccagcacct gcagcgtgag gtcgcagtcc
      841 tcgatgctct ccaggaaccg gacgccctgg acgttgtggt ggaacccccc gatcccgtat
      901 tcccggcaga gttcgtcgag gtacccctcc ccgaggtggc cgtatggtgt gtgaccgatg
      961 tcgtgaccga gagcgatcgc ctcgatcagg tcttcgttca ggccgagagc cctgccgatc
     1021 gtccgcccga tctttgatac cagctggacg tgcaggacgc ggtgggtgat gtggtcgttc
     1081 tcgaccaggt agaagacctg ggtcttgtcg a
//
LOCUS       DAOSZX010000188         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_97053_length_1090_cov_18.683092, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000188 DAOSZX010000000
VERSION     DAOSZX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97053_length_1090_cov_18.683092"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK196_08510"
     CDS             <1..268
                     /locus_tag="PK196_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HON41490.1"
                     /translation="KHFPDGSITSSIFAAMSATVLASAEAVCATVGIDRPSLVTISSK
                     GAAILIVGIDEVALITAVIDKPADLPGLERELLDIAARIREEVA"
     gene            268..912
                     /locus_tag="PK196_08515"
     CDS             268..912
                     /locus_tag="PK196_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HON41491.1"
                     /translation="MYTILIVDDSPMIVDVFATMLQRGGYNPITALSGEECLEVLKTK
                     NPPDLILLDIMMEPMDGWETLEKIKANPATRNIPVLMLTAKPLTPEEANEYGPYIEDY
                     ILKPTTHHQLYEAIERVLTRRHSIAADIERAREGGVDSRLIDEYERLSKSVDINRRLL
                     KILETTYNIKDARAGVGEDITRAIKSMATSIKIQEERLNQIRAQIKSLSGEQEA"
BASE COUNT          295 a          309 c          269 g          217 t
ORIGIN      
        1 caagcacttc cccgatggga gcattacgtc atccattttt gcagcaatga gtgcaactgt
       61 ccttgcatcg gccgaagcgg tctgcgcgac tgtaggtata gatcgaccat cgctggttac
      121 catctcctca aaaggcgctg caatccttat cgtgggtatc gacgaggtcg ctctgataac
      181 agcggtgatc gataagcctg cagatcttcc cggattagag agagaactgc ttgatatagc
      241 agccaggatc agagaggagg tggcatgatg tatacgatac tgatagtcga cgacagtcca
      301 atgatcgtcg atgtcttcgc gaccatgctg cagcgtgggg gttataaccc gataaccgcc
      361 ttaagtggag aagagtgcct tgaggttctg aagacaaaaa accctccgga cctgatcctt
      421 ctagacatca tgatggaacc tatggacgga tgggagacac tcgagaagat caaagccaac
      481 cccgcgacac gtaacatccc ggttctcatg ctgaccgcaa aacccctcac tcccgaagaa
      541 gcaaacgaat acggtcccta catcgaggac tacatactca agccaaccac ccaccatcag
      601 ctctacgagg ccatcgagcg cgtgctgaca cgccggcact ccatcgccgc cgatatcgaa
      661 cgagcacgcg aaggcggtgt tgattccagg cttattgatg agtacgaacg cctctcaaag
      721 agcgtcgata tcaaccgccg cctcttaaag atccttgaga ctacctacaa cattaaggat
      781 gcacgtgcag gcgttggtga ggacatcaca agagccataa agagcatggc tacaagcatc
      841 aagatccagg aagagcgact gaatcagata cgcgcccaga tcaagtcact atcgggggaa
      901 caggaagcct gaaccacggc acgccttata gaaccctcat ctccccgcgt ccagaattaa
      961 cccacatcca ggtcggtttc ctggcgttcc tgcttcccga tatagacgat tgcgatcgtc
     1021 agcaccaaaa tcgccgcgag tgtcatgcca atctcggcgg gttcgtggac ttctaccccg
     1081 aagatcaaca
//
LOCUS       DAOSZX010000189         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_97191_length_1089_cov_19.762089, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000189 DAOSZX010000000
VERSION     DAOSZX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97191_length_1089_cov_19.762089"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..970)
                     /locus_tag="PK196_08520"
     CDS             complement(38..970)
                     /locus_tag="PK196_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HON41492.1"
                     /translation="MSGWVTDFLVVADQIFILVLLIAAGYVAVSTKIVDPRATRGLSG
                     LLINITLPALIIASMQVPITSERLAGTETLFLATWVFFAFSFALAWAVTRVMPMTSSE
                     RGVFQFAIVFGNVGFMGFPVAQTLFGADSLFYVAIFNLVFNLLVFSVGIAMLTGEREG
                     GFDPRLLANPGIAASIAGFLLFLGSVEIPAPFIDAIDLLGGVTTPLAMIIVGAMLATF
                     PAREMVGDWRIWAASAVLLLAVPAAYCYLFSPVFADPLINGVMITMAAMPAAANTVIF
                     AEQYGANARLASQIVFVSTIGSLVTIPLVTTLLL"
BASE COUNT          246 a          315 c          362 g          166 t
ORIGIN      
        1 ccggattcac cggagccggg ccgtgtattc tcccgcccta gagcagcagc gtcgtcacca
       61 gcggaatcgt aacaagcgac ccgatcgtcg agacgaagac tatctgcgac gcaagcctgg
      121 cgttggcgcc gtactgctcc gcgaagatga cggtgtttgc agcggccggc atagcggcca
      181 tcgtgatcat gacaccgttg atgagggggt cggcaaagac cggggagaat aggtagcagt
      241 aggccgcagg gacggcgagg aggaggacgg cgctcgccgc ccagatccgc cagtcgccga
      301 ccatctcccg cgccgggaac gtcgcgagca tcgccccgac gatgatcatc gcaagcggcg
      361 tggtcacccc gccgaggagg tcgatcgcgt ctatgaacgg ggccggtatc tccaccgacc
      421 cgaggaagag gaggaacccg gcgatggaag ccgcgatacc ggggtttgca agcagtctcg
      481 ggtcaaatcc tccctcccgc tccccggtga gcatcgctat cccgaccgag aagacgagaa
      541 ggttgaagac gaggttgaag atggcgacgt agaagaggga gtcggcgcca aagagcgtct
      601 gggcgaccgg gaaccccatg aacccgacgt tcccgaagac gatcgcgaac tgaaagactc
      661 ccctctcgga agatgtcatc ggcatcaccc tcgtgacggc ccaggcgagg gcgaacgaga
      721 aggcaaagaa gacccaggtc gcgaggaaga gggtctcggt accggcgagg cgctcggagg
      781 tgatcggaac ctgcatggat gcgatgatca gcgccgggag ggtgatgttg atgaggagac
      841 ctgagagtcc ccgggtggcc cgggggtcca cgatctttgt tgagacggcg acgtagccgg
      901 cggcgatcag cagcaccagg atgaagatct ggtcagcgac aaccaggaaa tctgtaaccc
      961 agccactcat aaacggagac gctccctcct gacctagcag tgtggattct gataactatt
     1021 tacgattctg ccgcggggtg gcgtggtcta tctgaggcca tcgatccaat acgcaccccg
     1081 tttgacaaa
//
LOCUS       DAOSZX010000190         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_100274_length_1066_cov_19.293769, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000190 DAOSZX010000000
VERSION     DAOSZX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100274_length_1066_cov_19.293769"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..>1066)
                     /locus_tag="PK196_08525"
     CDS             complement(171..>1066)
                     /locus_tag="PK196_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON41493.1"
                     /translation="VDRVFRKVRNLTIRYISGETLFPLEVSQYDPWVIREALHNCIAH
                     QDYQQSARITVTERPGSLTFTNRGEFIPGTVLSVIERDVPPDRYRNPFLANAMVELKM
                     IDTIGSGIKRMFRIQRDRNFPMPDYDLDEEGVVRVTIPGRVIDENYTRMLVKRKELTL
                     TDVIALDKVQKGHPITPEERTILRKKKLIEGRYPNLYVSESVAAEIDTKADYIRKRPF
                     DRAHFKSMVVAYLRTYHEARLAEIEDLLLDKVSDALNEDQRRHFIKDLLQEMRKEGTI
                     KTIGKTKGAKWVLTPDPANGV"
BASE COUNT          219 a          319 c          264 g          264 t
ORIGIN      
        1 ggtgaagggg tgtcctgacc tgttacaaag tgaggtgtct attttgttca cactctccaa
       61 tcattcccca aattcgggtt tcaaaacacc ccacaaaaat tgttcatttt acaattcagt
      121 cagaaatatc tgaattcgtt tcgccaatct tcgctaatct ctcccgccaa tcatacccca
      181 ttggcaggat caggggtgag cacccacttc gcaccctttg tcttcccgat ggtcttgatc
      241 gtcccctcct tacgcatctc ctggagcaga tccttgatga aatggcgcct ctggtcctcg
      301 ttcagagcat cggaaacctt gtccagcagc aggtcttcaa tctccgccag cctcgcctca
      361 tggtacgtcc ggaggtacgc caccaccatg ctcttgaaat gcgccctgtc aaacggtctc
      421 ttccggatat aatcggcctt tgtatcgatc tcggccgcga cactctccga aacatacagg
      481 ttcggatatc gtccctcgat gagtttcttc ttccgcagaa tagtccgttc ctcaggagtg
      541 atgggatggc ccttctgcac cttgtcgagg gcgatcacat ccgtcagcgt cagttccttc
      601 cgtttgacga gcatccgggt gtagttctca tcaatcaccc gaccgggaat cgtgacccgc
      661 accacgccct cttcatcgag gtcataatca ggcatcggaa aattgcggtc ccgctgtatc
      721 cggaacatcc ttttgatccc gctgccgatg gtgtcgatca tcttcaactc gaccatcgca
      781 ttggcaagga atgggtttcg ataccgatca ggcggcacat cgcgctcgat cacgctcagc
      841 accgtcccgg gaataaactc gccgcggttg gtgaacgtca gggagcccgg cctctcggtc
      901 accgtgatgc gggccgactg ctggtagtcc tggtgggcga tgcagttgtg caacgcctca
      961 cggatcaccc aggggtcata ttgcgacact tccaggggga agagcgtttc ccctgagata
     1021 tagcggatgg tgaggtttcg gaccttgcga aaaacgcggt cgacgg
//
LOCUS       DAOSZX010000191         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_105710_length_1028_cov_23.199383, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000191 DAOSZX010000000
VERSION     DAOSZX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105710_length_1028_cov_23.199383"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..951)
                     /locus_tag="PK196_08530"
     CDS             complement(94..951)
                     /locus_tag="PK196_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON41494.1"
                     /translation="MLDITVIAFAHHKGGTGKTTSCLNVAGYLQKDGKRVLVVDCDPQ
                     ANATSGLGVDPERVEQSMYDVFMSVFEGFPDARITDIIVPTASGIYLAPATLDLVGVE
                     PYLYGIDDRAGILREALSPVRRTYDFILIDTPPSMGQFVINGLVAADHTVVTLDAGTF
                     ALNGMEALSMIFNDIREMIGEEVNADFAILTRWKGSDDSGGGLALFLKRIFSPASFAE
                     EERETERLKAFESEVRKKFRQVFVVHYSPLIYEAQQKGLPISHYAPESKPGRDYRAVA
                     TALESLGNK"
BASE COUNT          223 a          310 c          253 g          242 t
ORIGIN      
        1 ccctgctcct gtcctgtcat acccggcgca accggtccgg taaatagtgc ttttcttcca
       61 ggtttctcga tcatcggtca cacatcctca atcttattta tttccaaggc tctccagcgc
      121 agttgctact gcccgatagt ctctgcctgg tttgctctcc ggcgcatagt gggagatcgg
      181 gagccccttc tgctgcgcct catagattag aggcgagtaa tgaactacaa acacctgcct
      241 gaacttcttt ctcacctcag actcaaacgc cttcagacgc tccgtctcac gctcctcctc
      301 ggcaaaggat gcgggtgaaa agatccgttt cagaaagagc gccagaccgc ccccggagtc
      361 atcagatcct ttccaccgcg tcaggatggc aaaatctgcg ttgacctcct ctccgatcat
      421 ctcccggatg tcgttaaaga tcatcgacag cgcctccata ccgttgaggg caaaggtgcc
      481 ggcatcgagg gtgacgacgg tatggtcggc cgcgacaagg ccgttgatga caaactgacc
      541 catgctcggg ggtgtgtcaa tcaggatgaa gtcgtatgtg cgtcgtaccg ggctgagcgc
      601 ctccctgagt atccccgccc ggtcgtctat gccgtagaga tagggttcga ccccaaccag
      661 gtcaagggtc gcgggggcga gataaatccc agatgccgtg gggactatga tatcagtgat
      721 cctggcatct ggaaaaccct caaagacgct catgaagacg tcatacatgc tctgctcaac
      781 tctttccggg tcgacgccga gcccgctggt ggcgttggcc tgcggatcac agtccaccac
      841 cagaacacgt tttccatcct tctggaggta acctgcaacg ttaaggcagg aggtagtctt
      901 tcctgtacca cccttgtggt gggcgaacgc tataacagta atatccagca cccctcagta
      961 cctcttttga gacgtctgct cttaaatcta tttttcgtaa tcacatacct attccaaaca
     1021 ccagagcc
//
LOCUS       DAOSZX010000192         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_107235_length_1018_cov_36.332295, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000192 DAOSZX010000000
VERSION     DAOSZX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107235_length_1018_cov_36.332295"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..>1018)
                     /locus_tag="PK196_08535"
     CDS             complement(260..>1018)
                     /locus_tag="PK196_08535"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HON41495.1"
                     /translation="IARVMLQQIGSDRFCLANLEGKLFNIYDDLDDVPLQNSSVLKAI
                     TGDDMHYVEKKGRDAYEARIFCVHIFTCNRPPSTPERVQNDAAFWGRWEFITFPNYFE
                     VNPKWYDQVLTPATCSAYFNLVLEMALSIYQAGELPVKSSAYEVRDAWQTNSDPLYRF
                     ITENMDRSEAGYVLKDDAYSGFLNFARTENVPPSKIPPTPETFAKAVFKYGFAPARVR
                     VDGARVQVFRGYAWKSTSQYKPGGATNATLQGGA"
BASE COUNT          187 a          304 c          293 g          234 t
ORIGIN      
        1 gtcggccctc gaggatcgcc cgggcaacct ccggcgggaa ggtctcgaaa aactgctgcg
       61 aggcggcgga ggtcatgggc gcacctcttc acggcttgag gtgtccaggg tggtcagggt
      121 atttcgccac aactccttat tattctgatc tctctctttt tctcccatac gcgtagagta
      181 ggggtgttta ctctgaccac cctggacaaa atttgagaat atcggtgggc tgactgcccg
      241 ggctgcacgt gtgaccatct catgcacctc cttgaagggt cgcgttggtc gctccacccg
      301 gcttgtactg gctggtgctc ttccaggcat atcctctaaa cacctgcacc cgtgccccgt
      361 ccacccgaac ccgtgccgga gcaaaaccgt atttgaatac cgcctttgcg aaggtctcgg
      421 gagtcggtgg gatcttcgag ggcgggacgt tctccgtccg ggcgaagttc aggaacccgc
      481 tatatgcgtc gtccttcagg acgtacccgg cctcgctccg gtccatgttc tccgtgatga
      541 acctgtagag cggatcgctg ttcgtctgcc aggcgtcccg gacttcgtag gcgctcgatt
      601 taaccggcag ttctcccgcc tgatagatag atagggccat ctccaggacc aggttaaaat
      661 acgcgctgca cgtcgccggg gtaagcacct ggtcgtacca cttggggtta acctcaaagt
      721 agttcggaaa cgtgatgaac tcccaccggc cccaaaacgc cgcgtcgttc tgcacccgtt
      781 cgggggtcga tgggggcctg ttgcacgtga agatatgcac gcagaagatc cgggcttcgt
      841 atgcgtcgcg gcctttcttc tctacgtaat gcatatcatc gccggtgatc gccttcagga
      901 ccgacgagtt ttgaaggggc acgtcgtcga ggtcgtcata gatgttgaag agcttaccct
      961 ccaggttcgc caggcagaac cgatcgcttc cgatctgctg cagcatcacc cgggcgat
//
LOCUS       DAOSZX010000193         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_2048
            NODE_109983_length_1001_cov_14.757928, whole genome shotgun
            sequence.
ACCESSION   DAOSZX010000193 DAOSZX010000000
VERSION     DAOSZX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864115
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:28:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,708
            CDSs (total)                      :: 1,667
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 41
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 5 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109983_length_1001_cov_14.757928"
                     /isolate="AMR_MDS_2048"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..>1001)
                     /locus_tag="PK196_08540"
     CDS             complement(8..>1001)
                     /locus_tag="PK196_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HON41496.1"
                     /translation="EDSDVNALFETIKIYDELTERGEEVAIAVICGNHMNLIEGDRRV
                     ASDLDTVLKVTDATSCIVVADGAEDEYVMPVILSRVPVSSVRRVVVNQMPNLEGTYYI
                     LRRFLDDPKVSRIVLVPIGLAMLLYALGYLLGYPEVAIIVVVGVVGIYLLFKGMGIDE
                     FFEYLVHSLKASLHGGRFTFVSYIAAILLCIVGVIMGLVSLLEYYAPFGIVIQVLSFI
                     YGAVAWFTAAGLVASGGKIIDIFLNERETIQRVVALPFFILAIGAIAYGASVYILSIS
                     SDLPGFPISSSDGVVYIVVSIIGGLFCAFFGVYLQSFLGRLMRESEPVALKGDA"
BASE COUNT          268 a          293 c          254 g          186 t
ORIGIN      
        1 aactgcttca ggcatcccct ttcagcgcaa ccggttcaga ttctctcatc aaccgaccca
       61 ggaacgactg gaggtaaacg ccaaaaaaag cgcagaagag tccgcctatg atcgagacaa
      121 ctatgtagac caccccatcc gaactcgata tggggaaacc gggcagatcg ctgctgatcg
      181 agaggatgta gacgcttgca ccgtaagcga tggctccgat tgccaggatg aagaacggca
      241 gggcaaccac ccgctggatg gtctcacgct catttaagaa gatatcgatg atcttgcccc
      301 ccgatgcaac caggccggca gcggtgaacc atgctaccgc cccatatatg aatgacagaa
      361 cctggatgac aataccaaac ggcgcataat actcaagaag gctgacaagc cccattatga
      421 cgccgacgat gcatagcagg atggcagcga tgtaggagac aaaagtaaac ctgccgccgt
      481 ggagcgaggc tttaagcgag tgcaccaggt actcgaagaa ctcgtcaatc cccatgccct
      541 tgaagagcag gtagatcccg acgaccccga caacgacgat gattgcaacc tcggggtatc
      601 caagcagata acccagcgcg taaaggagca tggcaagccc gatcggaaca agcacaatcc
      661 ttgagacctt cggatcgtcc aggaaacgtc tcaggatgta gtatgtcccc tccaggtttg
      721 gcatctggtt taccacaacc ctgcgcacgc tgctgaccgg caccctggac agaattaccg
      781 gcatgacata ctcatcctca gcaccgtccg ccaccacgat acaggatgtt gcatcggtca
      841 ccttgaggac tgtatcgagg tctgaagcca cccgccggtc gccctcgata aggttcatgt
      901 ggttgccgca gatgacagcg attgcaacct cctctccacg ctccgtgagt tcgtcataga
      961 tctttatggt ctcaaagagc gcgttgacat cggaatcctc g
//
